From ian.montgomery <@t> bio.gla.ac.uk Wed Nov 1 03:47:41 2006 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Wed Nov 1 03:47:51 2006 Subject: [Histonet] Leica TP1020 Message-ID: <002001c6fd9a$c5e2bdc0$4724d182@ibls.gla.ac.uk> After years hand processing I've finally got myself a Leica TP1020. But, I'm having a few teething problems and with the work piling up the problems are weighing heavily on my shoulders. Processing schedules, any hints and tips? I've Googled and searched the Histonet archive but without much success. The two major projects I have at the moment are salmon muscle and mouse brain. I've tried several schedules but the results are, **###*@@**, less than satisfactory. Any rapid fixes or is it back to the trusted hand processing and work out the TP1020 processing by trial and error. Ian. Dr. Ian Montgomery, Histotechnology, IBLS Support Services, Thomson Building, University of Glasgow, Tel:01413398855 Extn: 8511. From lpwenk <@t> sbcglobal.net Wed Nov 1 05:11:07 2006 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Wed Nov 1 05:11:16 2006 Subject: Fibrin stain Re: [Histonet] Serum Fiber in Blood In-Reply-To: <6.0.0.22.1.20061031093334.01b0cd10@gemini.msu.montana.edu> Message-ID: <001e01c6fda6$6e6ea020$9ce92d4b@HPPav2> If you like the PTAH stain, instead of the mercuric post-fixative, place it in Bouins in a 60 degree C. oven for 45-60 minutes, wash in running water 1-10 minutes until all the yellow is gone, and then stain as usual in the PTAH. This PTAH stain was on our HTL exam when I was a student, and the sat. mercuric chloride post-mordant was giving us splotchy stains. Same with using Zenker as the post-mordant. One of my classmates (Liz) found the Bouins post-mordant PTAH procedure in Ann Preace's book. We tried it, it worked better than the mercuric chloride, and we've been using this method at our hospital for the last 25 years. Works every time, no splotchiness, no mercury, and, if you're already doing trichromes, you already have the Bouins so there's nothing special to make up. If, on the other hand, you would like our lab's version of MSB (again, no mercury, basically a trichrome with martius yellow add to stain RBCs (quadrachrome?) - email me at my WORK email = pwenk@beaumont.edu Do NOT reply back to Histonet. That comes to my home, and the procedures are at work. MSB = collagen blue; RBC yellow; fibrin red (if young, yellow; if old, bluish); nuclei black to red; all other cells red (muscle, cytoplasm, etc). In other words, identical to a Masson Trichrome, but with yellow RBCs instead of red. Very good method for seeing the RBCs (yellow) inside the fibrin mesh (red). As compared with PTAH, where everything is blue except the collagen. Or Masson trichrome where RBCs and fibrin are both red. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Tuesday, October 31, 2006 11:36 AM To: Henry, Charlene; Histonet@lists.utsouthwestern.edu Subject: Fibrin stain Re: [Histonet] Serum Fiber in Blood Martius Yellow also known as MSB stain for fibrin works on FFPE. I will attach the method to you privately. You may also find this on a staining website. Peggy Wenk kindly sent this method to me so a thank you goes out to her. At 08:51 AM 10/31/2006, you wrote: >Our mouse pathologist has previously ordered the PTAH stain for blood >serum fiber in mouse tissues; however we plan to retire this stain >because of the mercury used in the stain. This is the last place in the >lab that requires mercury. My question is: do any of you know of >another special stain or antibody that will detect blood serum fiber in >FFPE mouse tissue? >Thanks, >Charlene > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jacqueline.Malam <@t> rli.mbht.nhs.uk Wed Nov 1 05:32:58 2006 From: Jacqueline.Malam <@t> rli.mbht.nhs.uk (Malam Jacqueline) Date: Wed Nov 1 05:36:54 2006 Subject: [Histonet] Melanin bleach Message-ID: We use 10% hydrogen peroxide for 24 hours at room temp and that works well. Although it is slower than Mallory bleach, it does allows a fuller range of antibodies to be used. See : British Journal of Biomedical Science 1999; 56: 188-193 Jacqui lancaster DISCLAIMER: This e-mail is confidential and privileged. If you are not the intended recipient please accept our apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform postmaster@rli.mbht.nhs.uk that this message has gone astray before deleting it. Comments or opinions expressed in this email are those of their respective contributors only. The views expressed do not represent the views of the Trust, its management or employees. University Hospitals of Morecambe Bay NHS Trust is not responsible and disclaims any and all liability for the content of comments written within.Thank you for your co-operation. From mparker <@t> epl-inc.com Wed Nov 1 06:07:43 2006 From: mparker <@t> epl-inc.com (Mary Parker) Date: Wed Nov 1 06:08:28 2006 Subject: [Histonet] Ink, slides, cassettes Message-ID: If you are having trouble with markers fading, try Mercedes Platinum Line Markers, catalog # MER308W. We haven't had any problems with them, and they have a very fine tip. Mary D. Parker Histology Lab Manager EPL, Inc. PO Box 12766 RTP, N.C. 27709 919 998-9407 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WWmn916@aol.com Sent: Tuesday, October 31, 2006 10:37 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Ink, slides, cassettes Hello everyone, Two questions: I know this question comes up every now and then, but has anyone else been having problems with cassettes not holding the print from a Surgipath cassette printer? Cassettes, and not all colors, are fading drastically after coming off the processor and in some cases looks like runny, blobs of ink. We also seem to be having some problems with handwritten slides fading drastically after staining. We've used Securline pens for a long time and now they aren't doing so well on slides, especially the Superfrost Plus slides. The Marketlab pens seem to do a little better with legibility but smear badly if your fingers are slightly wet....and they don't last long. Thanks, Deb King Sacramento, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nancy.troiano <@t> yale.edu Wed Nov 1 06:28:02 2006 From: nancy.troiano <@t> yale.edu (Nancy W. Troiano) Date: Wed Nov 1 06:28:13 2006 Subject: [Histonet] workshop on unusual beasties Message-ID: <5.2.1.1.2.20061101072559.00c5ce88@email.med.yale.edu> We've submitted an abstract for a workshop at the NSH convention in Denver next year on GMA and MMA embedding techniques which can be applies to a whole array of unusual beasties from horseshoe crabs to whales to mice to paintchips! If it gets accepted this may provide you with some information on processing a wide variety of beasties for histological purposes. From barry_m <@t> ozemail.com.au Wed Nov 1 06:41:59 2006 From: barry_m <@t> ozemail.com.au (Barry Madigan) Date: Wed Nov 1 06:42:14 2006 Subject: [Histonet] HER2 CISH Message-ID: <001201c6fdb3$2045daa0$0201010a@WORKSTATION1> Is anyone outside of Australia doing HER2 CISH on Breast tissue at all? Regards Barry Madigan Immunohistochemistry QHPS- Central Royal Brisbane Hospital Australia From sbreeden <@t> nmda.nmsu.edu Wed Nov 1 07:01:50 2006 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Nov 1 07:02:08 2006 Subject: [Histonet] Paraffin Dispenser Help Message-ID: <4D14F0FC9316DD41972D5F03C070908B0DB2FC@nmdamailsvr.nmda.ad.nmsu.edu> We just purchased an XH-4002, 2.5 gallon paraffin dispenser. If anyone has used one of these units, could you please contact me to give your help with temperature adjustment? The book that accompanied the unit has absolutely no correlation with the unit itself and I'm having trouble getting the temperature adjusted. Thanks in advance. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 From RBARNHART <@t> summithealth.org Wed Nov 1 08:15:29 2006 From: RBARNHART <@t> summithealth.org (Rebecca Barnhart) Date: Wed Nov 1 08:16:06 2006 Subject: [Histonet] Pathologist/Medical Director job description Message-ID: We are in the process of getting a new pathologist/medical director and have discovered that we do not have a job description. If anyone would be willing to share their facility pathologist/medical director job description I would so grateful. Thanks in advance. Becky From benoit.delatour <@t> ibaic.u-psud.fr Wed Nov 1 08:19:13 2006 From: benoit.delatour <@t> ibaic.u-psud.fr (Delatour =?iso-8859-1?Q?Beno=EEt?=) Date: Wed Nov 1 08:19:38 2006 Subject: [Histonet] Re: Timms stain in fresh frozen cryosections In-Reply-To: <200610311810.k9VI9e39024400@mail2.mail.u-psud.fr> References: <200610311810.k9VI9e39024400@mail2.mail.u-psud.fr> Message-ID: <6.0.1.1.2.20061101145556.02a2dcd8@mailhost.pop.u-psud.fr> Hi Anna, You can simply incubate mounted slices in a 1.2% NaS.9H2O solution (in buffer w/v : 0.6 g / 50 cc of PB 0.1M) for 30 min. and then, after rinsings, proceed with TIMM staining. It works well with the limit that cutting fresh unfixed brains and doing repeated incubations and baths is risky (tissue goes away!). To minimize this, cut thin (<10?m) and slowly. Let the sections dry. Other alternatives 1) mice are alive : perfuse them with Na2S followed by fixative ; gives excellent results. 2) brains have not yet been cut : the simplest method for lazy people is to do "immersion autometallography" by incubating tissue in a solution of Na2S, then fixation in formadehyde. Once slices are obtained, proceed to TIMM staining. Beno?t --------"If you think research is expensive, try disease"-------- B. Delatour Laboratoire de Neurobiologie de l'Apprentissage, de la M?moire & de la Communication, NAMC, CNRS UMR 8620, B?t 446 Universit? Paris-Sud 91405 Orsay Cedex, FRANCE Tel:33 (0)1 69 15 49 88 Fax:33 (0)1 69 15 77 26 Mobile:0682991092 Email benoit.delatour@ibaic.u-psud.fr Web http://www.namc.u-psud.fr From denise.woodward <@t> uconn.edu Wed Nov 1 09:06:37 2006 From: denise.woodward <@t> uconn.edu (Woodward, Denise) Date: Wed Nov 1 09:06:53 2006 Subject: [Histonet] Connecticut Society Fall Meeting Message-ID: The Connecticut Society Fall Meeting will be held Saturday, November 11th at Yale University School of Medicine in New Haven Connecticut. The 2 presentations are: Implementing the Sakura Rapid Tissue Processor and Osteoarthritis Research. For more information, please go to this website www.nshRegion1.org and click on "Connecticut" for a complete brochure including registration forms and directions to the Lecture center. Walk-Ins are welcome! From jkiernan <@t> uwo.ca Wed Nov 1 09:23:32 2006 From: jkiernan <@t> uwo.ca (John A. Kiernan) Date: Wed Nov 1 09:23:38 2006 Subject: [Histonet] TRAP staining on undecalcified bone References: <000f01c6fd3f$5c67c7b0$4b00a8c0@gucancer.local> Message-ID: <4548BBF4.AB4DCFD1@uwo.ca> Is TRAP is tartrate-resistant acid phosphatase (an enzyme in osteoclasts; also in osteoblasts near resorbing bone surfaces)? If so, try following up this internet link: http://www.histosearch.com/histonet/Feb01/Re.TRAPstainA.html Unfortunately items from the histosearch.com archives (Histonet questions and answers) are undated, but none can be more than about 10 years old! The author of the above link is a bone expert; she edits the "Hard Times" NSH newsletter. John Kiernan Anatomy, UWO London, Canada ______________________________ Tiffany Pitts wrote: > > Hello all, > I am trying to do TRAP staining on undecalcified mouse bones cut from > Methylmethacrylate embedded blocks. My problem is that I cannot seem to find > a protocol that works well and/or is on the market any more. Does anyone > know of a protocol I can use or (even better) a kit? Our in-house protocol > is light on details and I'm not sure if I'm doing it right. > Thank you > > Tiffany Pitts > Department of Urology > University of Washington > Seattle, WA > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Wed Nov 1 09:40:22 2006 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Nov 1 09:40:41 2006 Subject: [Histonet] TRAP staining on undecalcified bone In-Reply-To: <4548BBF4.AB4DCFD1@uwo.ca> Message-ID: <003a01c6fdcc$0b997580$6501a8c0@Patsy> Tiffany, Sigma still sells a kit for TRAP the enzyme histochemical reaction cat. No. 387-A Leukocyte Acid Phosphatase this is a kit. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John A. Kiernan Sent: Wednesday, November 01, 2006 8:24 AM To: Tiffany Pitts Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] TRAP staining on undecalcified bone Is TRAP is tartrate-resistant acid phosphatase (an enzyme in osteoclasts; also in osteoblasts near resorbing bone surfaces)? If so, try following up this internet link: http://www.histosearch.com/histonet/Feb01/Re.TRAPstainA.html Unfortunately items from the histosearch.com archives (Histonet questions and answers) are undated, but none can be more than about 10 years old! The author of the above link is a bone expert; she edits the "Hard Times" NSH newsletter. John Kiernan Anatomy, UWO London, Canada ______________________________ Tiffany Pitts wrote: > > Hello all, > I am trying to do TRAP staining on undecalcified mouse bones cut from > Methylmethacrylate embedded blocks. My problem is that I cannot seem to find > a protocol that works well and/or is on the market any more. Does anyone > know of a protocol I can use or (even better) a kit? Our in-house protocol > is light on details and I'm not sure if I'm doing it right. > Thank you > > Tiffany Pitts > Department of Urology > University of Washington > Seattle, WA > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Wed Nov 1 09:56:40 2006 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Wed Nov 1 09:56:54 2006 Subject: [Histonet] Autofluorescence Message-ID: All, When marking tiny biopsies to be cut for Immunofluorescence, what is everyone's preference for dyes that do not exhibit autofluorescence? Thanx In Advance, Glen Dawson Milwaukee, WI From Tamara.Franz-Odendaal <@t> msvu.ca Wed Nov 1 09:55:33 2006 From: Tamara.Franz-Odendaal <@t> msvu.ca (Tamara Franz-Odendaal) Date: Wed Nov 1 10:18:25 2006 Subject: [Histonet] TRAP staining on undecalcified bone Message-ID: Hi We have done TRAP staining in our lab on undecalcified bone. Refer to this paper for a great protocol. Witten, PE., Hansen A., Hall, BK. Journal of Morphology 250: 197-207. Tamara Tamara Franz-Odendaal (PhD) Biology Dept, Mount Saint Vincent University 166 Bedford Highway Halifax, NS, B3M 2J6 Canada Tel: 902 - 457 6140 Fax: 902 - 457 6455 >>> "Tiffany Pitts" 10/31/2006 6:53 PM >>> Hello all, I am trying to do TRAP staining on undecalcified mouse bones cut from Methylmethacrylate embedded blocks. My problem is that I cannot seem to find a protocol that works well and/or is on the market any more. Does anyone know of a protocol I can use or (even better) a kit? Our in-house protocol is light on details and I'm not sure if I'm doing it right. Thank you Tiffany Pitts Department of Urology University of Washington Seattle, WA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LRaff <@t> lab.uropartners.com Wed Nov 1 10:28:14 2006 From: LRaff <@t> lab.uropartners.com (Lester Raff) Date: Wed Nov 1 10:28:30 2006 Subject: [Histonet] Formalin Substitutes Message-ID: <5DA1CA5D0B98A84985B545A24423B822019A4D@UPLAB01.uplab.local> Hello 'netters: Anyone with experience using Surgipath IBF in docs offices for small biopsies? How are the results? Immunos ok? Do you use it in your processor as well? If you have IBF in the processor, can you accept specimens fixed in regular formalin? We have found one paper in Archives of Path that is very positive, but would like more feedback. Thanks, Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 From Wanda.Smith <@t> HCAhealthcare.com Wed Nov 1 10:30:35 2006 From: Wanda.Smith <@t> HCAhealthcare.com (Smith Wanda) Date: Wed Nov 1 10:31:02 2006 Subject: [Histonet] Ink, slides, cassettes In-Reply-To: Message-ID: Dear Deb, Our experience has been that the colored Histoscreen cassettes do not stamp as darkly as the Tissue Tek white cassettes. I sometimes stamp the Histoscreens twice to get a darker stamp. The instructions state that the printhead can be set to print the number 3 times, but to not exceed 3X each print. If you have changed the ribbon recently, there may be a problem with the pins in the stamp head. Our Biomed department works on our cassette printer. Surgipath and StatLab carry a fine tipped pen that seems to work well and does not dry out or smear (if your hands are not wet). Hope that helps!! Wanda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WWmn916@aol.com Sent: Tuesday, October 31, 2006 10:37 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Ink, slides, cassettes Hello everyone, Two questions: I know this question comes up every now and then, but has anyone else been having problems with cassettes not holding the print from a Surgipath cassette printer? Cassettes, and not all colors, are fading drastically after coming off the processor and in some cases looks like runny, blobs of ink. We also seem to be having some problems with handwritten slides fading drastically after staining. We've used Securline pens for a long time and now they aren't doing so well on slides, especially the Superfrost Plus slides. The Marketlab pens seem to do a little better with legibility but smear badly if your fingers are slightly wet....and they don't last long. Thanks, Deb King Sacramento, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Nov 1 10:30:50 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Nov 1 10:31:04 2006 Subject: [Histonet] Autofluorescence In-Reply-To: Message-ID: <20061101163050.91949.qmail@web61225.mail.yahoo.com> India ink. Ren? J. "Dawson, Glen" wrote: All, When marking tiny biopsies to be cut for Immunofluorescence, what is everyone's preference for dyes that do not exhibit autofluorescence? Thanx In Advance, Glen Dawson Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Want to start your own business? Learn how on Yahoo! Small Business. From hej01 <@t> health.state.ny.us Wed Nov 1 10:38:23 2006 From: hej01 <@t> health.state.ny.us (Helen E Johnson) Date: Wed Nov 1 10:38:56 2006 Subject: [Histonet] Formalin Fixation & IHC Message-ID: Hi Histonetters, In order to avoid overfixation of specimens in Formalin when performing IHC , specimens are usually transferred to 70% EtOH at 24 hrs. Is there any adverse effects of antigen damage in long term storage in EtOH? Will specimens get hard if kept long in EtOH? Helen Johnson (hej01@health.state.ny.us) From srishan <@t> mail.holyname.org Wed Nov 1 10:49:20 2006 From: srishan <@t> mail.holyname.org (srishan@mail.holyname.org) Date: Wed Nov 1 10:49:49 2006 Subject: [Histonet] scalpel removal system Message-ID: Hi, Can some one give me input on scalpel removal similar to mopec product. We do have the one produced by mopec (qlicksmart) which only takes care of the # 22 blades. I am looking for one which can take care of the # 60 blades. Thanks Nirmala From gcallis <@t> montana.edu Wed Nov 1 11:33:30 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Nov 1 11:33:45 2006 Subject: [Histonet] TRAP staining on undecalcified bone _publication In-Reply-To: References: Message-ID: <6.0.0.22.1.20061101103211.01b02c50@gemini.msu.montana.edu> Tamara, Thank you, the article is superb with excellent photographs. At 09:28 AM 11/1/2006, you wrote: >The year for the TRAP staining protocol is 2001. > >Witten, PE., Hansen A., Hall, BK. 2001. Journal of Morphology 250: >197-207. > >Tamara Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Nov 1 02:12:17 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Nov 1 11:41:42 2006 Subject: [Histonet] steamed about steamer-resolution Message-ID: <86ADE4EB583CE64799A9924684A0FBBFAFC8A8@wahtntex2.waht.swest.nhs.uk> So I was correct, the steam wasn't hot enough!!!!! Eat your words Terry, I am the man!!!! Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Prayer is a radical response to the mysteries of life. --Matthew Fox This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From cdemarinis <@t> SARATOGACARE.ORG Wed Nov 1 13:03:29 2006 From: cdemarinis <@t> SARATOGACARE.ORG (Demarinis, Carolyn) Date: Wed Nov 1 13:03:48 2006 Subject: [Histonet] bone marrow clot Message-ID: We receive bone marrow specimens collected by oncologists in their office. The bone marrow CLOT is immediately put in Bouin's fixative, fixed overnight and processed in a paraffin block. We are having a very difficult time cutting sections from the clot specimen. We have no problem cutting the bone biopsy. I have been told the difficulty might be caused by the oncologist using heparanized syringes. Does anyone have any suggestions that might help us prepare acceptable slides from the clot? Thanks. From jengirl1014 <@t> yahoo.com Wed Nov 1 13:05:36 2006 From: jengirl1014 <@t> yahoo.com (Jennifer Sipes) Date: Wed Nov 1 13:05:53 2006 Subject: [Histonet] Need some help Message-ID: <20061101190536.44369.qmail@web62404.mail.re1.yahoo.com> You guys have always been there for me before....here's another quick question! I'm self taught when it comes to histology and with your help have managed to become really good at my job. There have been some suggestions made and one of them was to take a histology course to have an actual class under my belt. The only problem is I can't seem to find any at Johns Hopkins University(where I work). I can't really afford to take one at another University. Does anyone have any suggestions or ideas? I'd greatly appreciate. Thanks a bunch, Jennifer Sipes From karenadams <@t> comcast.net Wed Nov 1 13:49:32 2006 From: karenadams <@t> comcast.net (karenadams@comcast.net) Date: Wed Nov 1 13:49:49 2006 Subject: [Histonet] initiating immunostaing Message-ID: <110120061949.10310.4548FA4C0001BFDF0000284622070210539C030E0B0E020A9D0E05@comcast.net> Anyone willing to provide any advice/resource links for a small private path lab wishing to advance into this century and begin to perform our own immunohistochemistry stains. We are presently sending these (mostly tumor markers, hematopoietics and intermediate filaments) to a competitor. Any and all input would be greatly appreciated! Thanks, Karen From gcallis <@t> montana.edu Wed Nov 1 13:56:09 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Nov 1 13:56:22 2006 Subject: Histotechnology schools on the web Re: [Histonet] Need some help In-Reply-To: <20061101190536.44369.qmail@web62404.mail.re1.yahoo.com> References: <20061101190536.44369.qmail@web62404.mail.re1.yahoo.com> Message-ID: <6.0.0.22.1.20061101125301.01b44e58@gemini.msu.montana.edu> Jennifer Go to the NSH website, then schools of histotechnology. There are some which are website based so you can still work and take classes towards a registry. Good luck At 12:05 PM 11/1/2006, you wrote: >You guys have always been there for me before....here's another quick >question! > >I'm self taught when it comes to histology and with your help have managed >to become really good at my job. There have been some suggestions made >and one of them was to take a histology course to have an actual class >under my belt. The only problem is I can't seem to find any at Johns >Hopkins University(where I work). I can't really afford to take one at >another University. Does anyone have any suggestions or ideas? I'd >greatly appreciate. > >Thanks a bunch, > >Jennifer Sipes > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From hymclab <@t> hyhc.com Wed Nov 1 14:18:59 2006 From: hymclab <@t> hyhc.com (hymclab) Date: Wed Nov 1 14:16:17 2006 Subject: [Histonet] scalpel removal system Message-ID: I've been bothering Mopec to get a 60 blade Click Smart just like the 20 blade for years!!!!! No luck so far!!!! Please post if you have found a similar device for 60 blades. Thanks, Dawn D. Schneider, HT(ASCP) Howard Young Medical Center Woodruff, WI 54568 (715)356-8174 schneiderd@hyhc.com -----Original Message----- From: srishan@mail.holyname.org [mailto:srishan@mail.holyname.org] Sent: Wednesday, November 01, 2006 10:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] scalpel removal system Hi, Can some one give me input on scalpel removal similar to mopec product. We do have the one produced by mopec (qlicksmart) which only takes care of the # 22 blades. I am looking for one which can take care of the # 60 blades. Thanks Nirmala _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From bhewlett <@t> cogeco.ca Wed Nov 1 14:55:03 2006 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Wed Nov 1 14:55:14 2006 Subject: [Histonet] Formalin Fixation & IHC References: Message-ID: <007401c6fdf8$013adf30$6500a8c0@mainbox> Hi Helen, "Overfixation" was a term used by early histologists to describe the unexpected and excessive hardening and shrinkage of tissues which occurred following lengthy exposure to certain fixatives. It gave rise to the concept of tolerant and intolerant fixatives. Heidenhain's SUSA and Zenker's fluid are examples of intolerant fixatives and textbooks usually suggest that fixation times should not exceed 24 hours. On the other hand, an aqueous solution of formaldehyde is the classic example of a tolerant fixative, tissues may be left in it for extended periods of time, with no excessive hardening or shrinkage. So-called 'overfixation', as applied to fixation of tissues in aqueous solutions of formaldehyde for IHC, is a total misnomer. Simply put, it never occurs in any clinically relevant time frame (months!!!). Formaldehyde fixes by formation of hydroxymethyl adducts on reactive side chains of proteins. Once sufficient adducts are formed, they slowly cross-link to stabilize the proteins in a gel-like formation. At room temperature, it takes approximately 24 hours for maximal binding of formaldehyde to occur and hence all the adducts to form. These initial adducts, and any initial cross-links formed, are unstable and easily reversed. For tissues fixed for 24 hours, the cross-links are largely reversed by washing ( in water or 70% EtOH) after a few hours. It probably takes at least 5-7 days for most of the cross-links to form, and a small amount of cross-linking still continues over time. Even after fixation for 7 days or more, the cross-links can still be reversed by prolonged washing. That's great for IHC, since cross-links can be reversed by HIER (which is just washing at elevated temperatures!). We have tested over 300 antibodies, using human tissues fixed in NBF for times ranging from 2 hours to 3 months ( some out to 1 year). In no case were we unable to demonstrate the antigen by IHC in tissues fixed for 24 hours or longer. In contrast, many antigens (particularly surface proteins) failed to be demonstrated in the same tissues fixed for less than 24 hours!!! Don't worry about so-called 'overfixation', there is NO SUCH THING. Do worry about underfixation, it can be devastating to IHC. My advice, leave the tissues in formaldehyde for up to 90 days and then if you must, transfer to 70% EtOH for further storage. Regards, Bryan ----- Original Message ----- From: "Helen E Johnson" To: Sent: Wednesday, November 01, 2006 11:38 AM Subject: [Histonet] Formalin Fixation & IHC > > Hi Histonetters, > In order to avoid overfixation of specimens in Formalin when > performing IHC , specimens are usually transferred to 70% EtOH at 24 hrs. > Is there any adverse effects of antigen damage in long term storage in > EtOH? Will specimens get hard if kept long in EtOH? > Helen > Johnson (hej01@health.state.ny.us) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mtitford <@t> aol.com Wed Nov 1 15:04:02 2006 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Wed Nov 1 15:04:12 2006 Subject: [Histonet] Learning histology Message-ID: <8C8CC106454F2BF-F48-F408@MBLK-M33.sysops.aol.com> Jennifer Sipes asks about learning histology. Its not like attending a formal course but you can always sit down with a set of slides and a copy of Ham's Histology and Sheehan (second edition) and just work your way through the book. It really works! Your Anatomy Department (or whatever they call themselves now) can probably lend you a set of slides the freshman medical students have used in the past. Medical schools are getting away from slide sets now in favor of Internet based images but for histotechnologists it is invaluable to spend the time to look at the tissues under the microscope, and, having studied the structure, Sheehans book will tell you the special stains available to demonstrate the different structures. Good luck! Mike Titford USA Pathology Mobile AL USA ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From AnthonyH <@t> chw.edu.au Wed Nov 1 15:55:32 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Nov 1 15:57:14 2006 Subject: [Histonet] Serum Fiber in Blood Message-ID: Have you tried this PTAH that does not require mercury: Phosphotungstic Acid Haematoxylin Principle: Cherukian's modification employs an eosin solution that stains the erythrocytes red and differentiates them from the blue fibrin. Fixation: 10% buffered formalin. Microtomy: paraffin sections at 5?m. Controls: Use brain sections and section of muscle. A good stain will demonstrate the dendrites as blue where as in a bad stain they appear light grey to salmon in colour. Nuclei, fibrin, platelets and muscle will be blue, red cells and collagen appear red. Muscle striations should be well defined. Reagents: 1. Stock Eosin: - Warning: Flammable - see MSDS Eosin Y, water soluble (CI 45380) 0.5g Distilled Water 10ml 80% Ethanol 190ml 2. Working Eosin Solution: Stock Eosin 10ml Before use add 50ul glacial acetic acid. 3. 1% Periodic Acid 4. PTAH solution Haematoxylin (CI 75290) 0.5g Phosphotungstic Acid 10g Distilled water 500ml Dissolve solid ingredients in separate portions of the water. Use gentle heat for Haematoxylin. Combine solutions when cool. Add 0.088g potassium permanganate to ripen. The stain is ready to use. Procedure: 1. Dewax and hydrate sections to 80% alcohol. 2. Place slides in working eosin solution for 30 seconds. 3. Wash slides in distilled water for a few seconds. 4. Place slides in 1% periodic acid for 20 minutes. 5. Wash slides in water for 3 minutes. 6. Place slides in PTAH solution for 30-90 minutes in 60oC oven. Check from 30 minutes on. 7. Dehydrate, clear and mount. Results: Dendrites, nuclei, fibrin, platelets and muscle - blue Red blood cells and collagen - red. Reference: 1. Cherukian, C.J., Histologic. 8(4); 105, (1977). 2. Luna, L., Histologic. 5(2); 66, (1975). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Henry, Charlene Sent: Wednesday, 1 November 2006 2:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Serum Fiber in Blood Our mouse pathologist has previously ordered the PTAH stain for blood serum fiber in mouse tissues; however we plan to retire this stain because of the mercury used in the stain. This is the last place in the lab that requires mercury. My question is: do any of you know of another special stain or antibody that will detect blood serum fiber in FFPE mouse tissue? Thanks, Charlene _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From histology.bc <@t> shaw.ca Wed Nov 1 16:31:15 2006 From: histology.bc <@t> shaw.ca (Paul Bradbury) Date: Wed Nov 1 16:32:21 2006 Subject: [Histonet] Bone marrow clot In-Reply-To: References: Message-ID: <45492033.6010401@shaw.ca> Hi Carolyn, A few comments regarding your bone marrow problems. Bouin's fixative is a poor choice for fixing any bone marrow specimen. The bone marrow clot reacts like any other clot and when fixed in Bouin will become very brittle after processing. A second (and major) effect of Bouin is to lyse most of the cytoplasmic granules present in the bone marrow cells. As any hematologist will confirm, these granules are a vital element in the recognition of the different cell lines in the bone marrow. A much better choice for both the core biopsy and the aspirate would be B-5 or one of the zinc-based substitutes. Any coagulant fixative (such as Bouin, B-5 or a zinc-based solution) should not the left to fix overnight or the tissues will become brittle. For a 4 mm bone core, around 2 hours at room temperature should be adequate; for a bone marrow clot, the time will vary according to the size, but for a clot of up to 4 mm thick, 3 hours should be ample. The core should be decalcified as usual (30 - 40 minutes in Cal-Ex is usually enough), then processed on a routine cycle. We routinely cut bone marrow cores and aspirates at 1.5 microns without any problems. This thickness (or thin-ness) gives beautiful clarity to the bone marrow cells. B-5 will leave the usual mercury pigment. but this is easily removed with an iodine-thiosulphate treatment. The use of heparinized syringes will not have any adverse effects on the quality of the tissue sample. The only effect they have is to prevent the aspirate material from clotting in the needle or the syringe. Paul Bradbury Kamloops, BC Canada Demarinis, Carolyn wrote: >We receive bone marrow specimens collected by oncologists in their >office. > >The bone marrow CLOT is immediately put in Bouin's fixative, fixed >overnight > >and processed in a paraffin block. > >We are having a very difficult time cutting sections from the clot >specimen. > >We have no problem cutting the bone biopsy. > >I have been told the difficulty might be caused by the oncologist using >heparanized syringes. > >Does anyone have any suggestions that might help us prepare acceptable >slides from the clot? > >Thanks. > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > From liz <@t> premierlab.com Wed Nov 1 17:10:16 2006 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Nov 1 17:01:53 2006 Subject: [Histonet] intra cellular calcium Message-ID: <000a01c6fe0a$e4c631c0$0300a8c0@domain.Premier> I know this might be a bit of a silly question, but we have been running a lot of von kossa on m. tuberculosis infected guinea pig lungs. The pathologist is telling us that he sees deposistion of calcium that is extra cellular but not intra cellular and was wondering if the von kossa does stain intra cellular calcium. My general answer to that would be yes, but I decided to post this question to the Histonet to see if anyone had any info they were willing to share. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 From AnthonyH <@t> chw.edu.au Wed Nov 1 17:04:17 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Nov 1 17:06:00 2006 Subject: [Histonet] Leica TP1020 Message-ID: Ian, Here are our programs. The Brain blocks have been fixed for several weeks prior to processing, hence the short processor fixation time. You will probably need to vary the dehydration and clearing times for your tissues: The Leica TP1020 is a carousel type tissue processor with agitation, vacuum and fume extraction. Routine Processing Programs Program 1 2 3 4 5 Station Solution Routine-Overnight,Two-Basket-Routine, Urgent, Routine-Weekend,Brain 1 Formalin 1:00 1:15 0:10 1:30 (No Vacuum) 10 min 2 Formalin 1:00 1:15 0:20 1:30 10 min 3 70% Ethanol 0:30 1:15 0:05 1:00 1:00 4 Absolute Ethanol 1:30 1:15 0:10 2:00 4:00 5 Absolute Ethanol 1:30 1:15 0:10 2:00 4:00 6 Absolute Ethanol 2:15 1:15 0:10 2:00 4:00 7 Xylene 0:30 1:15 0:10 1:30 2:00 8 Xylene 1:00 1:15 0:10 1:30 2:00 9 Xylene 1:30 1:15 0:10 1:30 2:00 10 Wax 1:00 1:15 0:05 1:30 2:00 11 Wax 1:00 1:15 0:10 1:30 3:00 12 Wax 2:00 1:15 0:10 2:00 3:00 Total Time 14:57 15:12 2:17 19:42 27:20 Immediate Program Start 1. Press (Program displayed always last one used) 2. Choose Program using <+> or <-> Keys (see above) 3. Press to begin program Urgent Program Start 1. For programs less than 8 hours duration a warning code will appear 2. Check that the wax in the paraffin stations is molten, and if so: 3. Press and simultaneously to override warning code and commence processing Delayed Start (Weekend) This program does not use vacuum until Step 2. To finish at 7am Monday Morning: 1. Press 2. Select Program 4 (using <+> or <-> Keys) 3. Press Key 4. Enter 2 as days of delay (<+> or <-> Keys) 5. Press Key for hours 6. Enter "11" (<+> or <-> Keys) - start at 11am Sunday Morning 7. Press to commence program To check if programmed starting time leads to an acceptable end of run time press Security To protect program settings from accidental changes, the key functions of the control panel can be locked. 1. Press and hold for 5 seconds 2. "LOCKED" will be displayed To unlock, Press and hold for 5 seconds. Pausing a Program to add more cassettes 1. Press 2. Press to raise basket (If vacuum is on, this might take some time) 3. Add cassettes 4. Press to return basket to station 5. To resume process press Stop/Abort a Program 1. Press 2. When "STOP?" message appears, to abort program, press again End of Process 1. "DONE" is displayed. 2. Alarm sound is heard every 30 seconds 3. Press any key to stop alarm Maintenance Please refer to Maintenance Manual and Instrument Instruction Manual. The solutions need to be changed after every five runs by the person on call. Daily Maintenance 1. Check liquid level in each station 2. Check quality of each fluid 3. Clean rims of all stations and seal of each lid 4. Wipe control panel clean After Five Runs 1. Discard first formalin, rotate and add fresh formalin to last formalin station (Station 2) 2. Discard 70% ethanol and add fresh 70% ethanol 3. Discard first absolute ethanol, rotate and add fresh absolute ethanol to last absolute ethanol station (Station 6) 4. Discard first xylene, rotate and add fresh xylene to last xylene station (Station 9) After Ten Runs 1. As per above maintenance plus 2. Discard first wax 3. Rotate wax stations 4. Add fresh molten wax to last wax station (station 12) Monthly 1. Lift carousel cover to upper end position 2. Clean carousel axle with a clean cloth 3. Apply a thin coat of machine oil If the machines are turned off for a period long enough to solidify the wax, it is important to place a rod, or something similar, to break the vacuum seals of the wax lids, otherwise the container can warp. Recycling * Xylenes can be recycled. * Alcohols to be collected and recycled, unless they contain xylene, then discard (ie alcohols after xylene in dewaxing and alcohol wash) When Processing Goes Wrong Tissue can appear so poorly processed, that the blocks smell of xylene or the clearing agent used in processing. In these cases, blocks can be melted and returned to fresh molten wax overnight to improve infiltration before embedding. If tissue appears soft at the embedding stage, then dehydration is often the problem. This is common if tissues are too thick, resulting in poor movement of processing fluids around and into the tissue. Another sign is the presence of indentations from the cassette and lid on the tissue. These tissues require reprocessing. While the tissue is still hot (ie the wax is still molten) blot the tissue dry, place it back in its cassette and place the cassette in 10% formalin for reprocessing. This procedure dramatically improve the results in at least 90% of cases. The excellent results are probably due to the protective nature of the wax present in the adequately processed portions of the block. This insulates the tissue from the harmful effects of ethanol on the adequately processed portions of the tissue preventing the tissue from becoming hard and brittle (Johnson 2003). Johnson (2003) Histologic 36(1):21-22. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian Montgomery Sent: Wednesday, 1 November 2006 8:48 PM To: Histonet Subject: [Histonet] Leica TP1020 After years hand processing I've finally got myself a Leica TP1020. But, I'm having a few teething problems and with the work piling up the problems are weighing heavily on my shoulders. Processing schedules, any hints and tips? I've Googled and searched the Histonet archive but without much success. The two major projects I have at the moment are salmon muscle and mouse brain. I've tried several schedules but the results are, **###*@@**, less than satisfactory. Any rapid fixes or is it back to the trusted hand processing and work out the TP1020 processing by trial and error. Ian. Dr. Ian Montgomery, Histotechnology, IBLS Support Services, Thomson Building, University of Glasgow, Tel:01413398855 Extn: 8511. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From djmr55 <@t> hotmail.com Thu Nov 2 04:50:45 2006 From: djmr55 <@t> hotmail.com (donna rossi) Date: Thu Nov 2 04:50:54 2006 Subject: [Histonet] pH of EDTA Message-ID: We are having a problem with EDTA pH 8.0 from LabVision. When we take the pH of the concentrate it is 8.O. When we dilute it as recommended it changes to 6.0. Lab Vision replaced our bottles but the new bottles also come out to 6.0 when diluted. We took the pH of the Distilled water here and got 7.1. What could be the problem.? Do we adjust the distilled water to a higher pH? How do we do that? Do we then need to validate all of our antibodies that use EDTA using the" new" EDTA? We have also had this problem with Zymed EDTA but not as radical a difference as the Labvision. It was suggested that we switch to ready-made EDTA but we have a quantity of this and do not have the room to store multiple big bottles of this retrieval buffer. Can someone give me a suggestion? Thanks, Donna, SRHS _________________________________________________________________ [1]Use your PC to make calls at very low rates References 1. http://g.msn.com/8HMAENUS/2749??PS=47575 From ctsblack <@t> capeheart.uct.ac.za Thu Nov 2 04:58:58 2006 From: ctsblack <@t> capeheart.uct.ac.za (Melanie Black) Date: Thu Nov 2 05:03:37 2006 Subject: [Histonet] Picro Sirius Supra Blue Message-ID: Dear All Does anybody know who I can order Picro Sirius Supra Blue from??? It is used in a connective tissue stain. Many Thanks Melanie Black -- Cardiovascular Research Unit Div. of Cardiothoracic Surgery Chris Barnard Building University of Cape Town Anzio Road Observatory 7925 Republic of South Africa Tel +27 21 406-6589 Cel +27 82 469-3352 Fax +27 21 448-5935 From bayoubelle311 <@t> gmail.com Thu Nov 2 10:37:46 2006 From: bayoubelle311 <@t> gmail.com (Missy) Date: Thu Nov 2 10:38:01 2006 Subject: [Histonet] rat myelofibrosis questions Message-ID: <398f02c20611020837m95c7738m875101c605050f4@mail.gmail.com> Hi all, Sorry to bother again, I just have a quick question. I am looking for myelofibrosis in rat, we had intended to look at the femur, however we got to talking and brainstorming and were wondering if the ilium would be a better place to look. Since the iliac crest is the location of bone marrow biopsy in human, and that way we can "double dip" on the animals and use the femur for another assay. Do you think it will work or has any one gotten positive results using the ilium? Also, regardless of wehter we use femur or ilium, which concentration of EDTA is optimum for decal? Missy From kimtournear <@t> yahoo.com Thu Nov 2 10:58:09 2006 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Thu Nov 2 10:58:24 2006 Subject: [Histonet] P16-melanoma...HELP Message-ID: <20061102165809.11421.qmail@web37701.mail.mud.yahoo.com> Hi, I work for a dermatology clinic. We also do our own pathology/histology....I have a question regarding the P16-Melaris test (blood draw) for Hereditary Cancer Genetic Testing....we have received the kit to draw the blood (purple tube), paper work, etc from the Vendor (Myriad)....We are CLIA regulated (for the histo lab as well), but I need to know if there are any specific rules (CLIA) to follow (other than a send out procedure) for the nurses to be able draw the blood from the patient? If I could get some help on this ASAP, I would greatly appreciate it. The vendor will be here for lunch today.... Thanks so much.... Kim Tournear, HT (ASCP), QIHC (ASCP) Specialists in Dermatology Tucson, AZ --------------------------------- We have the perfect Group for you. Check out the handy changes to Yahoo! Groups. From gcallis <@t> montana.edu Thu Nov 2 11:50:17 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Nov 2 11:50:41 2006 Subject: [Histonet] rat myelofibrosis questions In-Reply-To: <398f02c20611020837m95c7738m875101c605050f4@mail.gmail.com> References: <398f02c20611020837m95c7738m875101c605050f4@mail.gmail.com> Message-ID: <6.0.0.22.1.20061102103919.01b71188@gemini.msu.montana.edu> Missy, Concentration of EDTA will depend on which EDTA you use. EDTA, disodium or EDTA without any sodiums attached only goes into solution at around 10% or 10g/100 ml, pH 7.0 to 7.6 and people often have to use sodium hydroxide to make it go into solution which should also adjust the pH up to 7.0. If you use EDTA tetrasodium you can get the concentration very high at 14% or more, but you must adjust the pH down. We use glacial acetic acid for this purpose (bone expert Webb Jee used this EDTA decalcification method for bone). EDTA tetrasodium dissolved in water or buffer has a pH of approximately 9 to 11, so the pH should be adjusted down to pH 7.4 or so. If the pH remains very alkaline, then the alkaline sensitive protein linkages can be damaged. You may want to look at the femur too, since you could use the contralateral side (femur) for your other test. Have you done a literature search on mouse ilium just to see what is out there. Google Scholar is a good place to search if PUBMED becomes cumbersome. Sternum is another bone marrow biopsy site in humans - just a thought here. We used to do fibrin staining on acid decalcified bone marrow biopsies without problems rather than use the slower EDTA decalcification. If you useAt 09:37 AM 11/2/2006, you wrote: >Hi all, > >Sorry to bother again, I just have a quick question. I am looking for >myelofibrosis in rat, we had intended to look at the femur, however we got >to talking and brainstorming and were wondering if the ilium would be a >better place to look. Since the iliac crest is the location of bone marrow >biopsy in human, and that way we can "double dip" on the animals and use the >femur for another assay. Do you think it will work or has any one gotten >positive results using the ilium? >Also, regardless of wehter we use femur or ilium, which concentration of >EDTA is optimum for decal? > >Missy >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From TJJ <@t> Stowers-Institute.org Thu Nov 2 12:17:46 2006 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Thu Nov 2 12:19:52 2006 Subject: [Histonet] RE: intra cellular calcium References: Message-ID: Liz, Indeed the von Kossa stain will stain intracellular calcium. We have done these on cultured cells and demonstrated calcium using this technique. Teri Johnson Managing Director Histology Facility Stowers Institute for Medical Research Kansas City, MO 64110 From PMonfils <@t> Lifespan.org Thu Nov 2 12:37:09 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Nov 2 12:37:30 2006 Subject: [Histonet] intra cellular calcium Message-ID: <4EBFF65383B74D49995298C4976D1D5E273BA7@LSRIEXCH1.lsmaster.lifespan.org> The Von Kossa technique stains solid deposits of calcium salts. While hypercalcaemia is common in patients undergoing treatment for tuberculosis, resulting in elevated serum and cytoplasmic calcium levels, this is calcium in solution, which would not stain with Von Kossa. I' m not aware of any kind of intracellular calcium deposits that could be demonstrated with Von Kossa? > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Liz > Chlipala > Sent: Wednesday, November 1, 2006 3:10 PM > To: 'Histonet' > Subject: [Histonet] intra cellular calcium > > I know this might be a bit of a silly question, but we have been running a > lot of von kossa on m. tuberculosis infected guinea pig lungs. The > pathologist is telling us that he sees deposistion of calcium that is > extra > cellular but not intra cellular and was wondering if the von kossa does > stain intra cellular calcium. My general answer to that would be yes, but > I > decided to post this question to the Histonet to see if anyone had any > info > they were willing to share. > > > > Thanks in advance > > > > Liz > > > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > > Manager > > Premier Laboratory, LLC > > P.O. Box 18592 > > Boulder, CO 80308 > > phone (303) 735-5001 > > fax (303) 735-3540 > > liz@premierlab.com > > www.premierlab.com > > > > Ship to Address: > > > > Premier Laboratory, LLC > > University of Colorado at Boulder > > MCDB, Room A3B40 > > Boulder, CO 80309 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From amywsmd <@t> gmail.com Thu Nov 2 12:38:04 2006 From: amywsmd <@t> gmail.com (Amy Wagelie) Date: Thu Nov 2 12:38:19 2006 Subject: [Histonet] use of blocking peptide to test antibody specificity Message-ID: <60525eb10611021038y3a42100y2472032aed08147f@mail.gmail.com> Hi list- Does anyone have experience with testing for antibody specificity with a blocking peptide in the context of a rabbit anti-serum?? This antibody is one we generated in our lab by immunizing w/ a human GST fusion protein; the resultant anti-serum works beautifully for both Western and IHC. I now want to test for specificity (in IHC) by blocking with the immunizing protein; i.e., incubating the primary antibody w/ the protein for an hour (?) before staining. I have read that a 5 to 10-fold excess of peptide should be sufficient; I have about 400mcg of the immunizing protein left, but my problem is that I don't know how much specific anitbody is present in the anti-serum. I'm guessing I'll have to just guess--but the protein was a challenge to prepare and it's a pretty valuable commodity. Any suggestions? Thanks-Amy -- Amy Wagelie-Steffen, M.D. UCSD Allergy / Immunology Fellow amywsmd@gmail.com From Barry.R.Rittman <@t> uth.tmc.edu Thu Nov 2 13:04:38 2006 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Thu Nov 2 13:04:42 2006 Subject: [Histonet] rat myelofibrosis questions References: <398f02c20611020837m95c7738m875101c605050f4@mail.gmail.com> <6.0.0.22.1.20061102103919.01b71188@gemini.msu.montana.edu> Message-ID: I am a little confused so should put my thoughts down. EDTA has four possible chelation sites for chelating calcium and other ions. If you start with EDTA powder and adjust the pH upwards you will first get mono, then di, then tri and finally tetrasodium EDTA. EDTA powder by itself if not very soluble until the pH is adjusted. Most recipes start with the disodium salt. I forget the actual pHs but memory is that they are around 4.6, 6.5, 8.5 and 11 respectively (forgive me if incorrect but I am not at my office computer). Whenever you adjust the pH you alter the EDTA salts that are produced. So that at pH 7.0 or so you have a micture of disodium and trisodium. Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Gayle Callis Sent: Thu 11/2/2006 11:50 AM To: Missy; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] rat myelofibrosis questions Missy, Concentration of EDTA will depend on which EDTA you use. EDTA, disodium or EDTA without any sodiums attached only goes into solution at around 10% or 10g/100 ml, pH 7.0 to 7.6 and people often have to use sodium hydroxide to make it go into solution which should also adjust the pH up to 7.0. If you use EDTA tetrasodium you can get the concentration very high at 14% or more, but you must adjust the pH down. We use glacial acetic acid for this purpose (bone expert Webb Jee used this EDTA decalcification method for bone). EDTA tetrasodium dissolved in water or buffer has a pH of approximately 9 to 11, so the pH should be adjusted down to pH 7.4 or so. If the pH remains very alkaline, then the alkaline sensitive protein linkages can be damaged. You may want to look at the femur too, since you could use the contralateral side (femur) for your other test. Have you done a literature search on mouse ilium just to see what is out there. Google Scholar is a good place to search if PUBMED becomes cumbersome. Sternum is another bone marrow biopsy site in humans - just a thought here. We used to do fibrin staining on acid decalcified bone marrow biopsies without problems rather than use the slower EDTA decalcification. If you useAt 09:37 AM 11/2/2006, you wrote: >Hi all, > >Sorry to bother again, I just have a quick question. I am looking for >myelofibrosis in rat, we had intended to look at the femur, however we got >to talking and brainstorming and were wondering if the ilium would be a >better place to look. Since the iliac crest is the location of bone marrow >biopsy in human, and that way we can "double dip" on the animals and use the >femur for another assay. Do you think it will work or has any one gotten >positive results using the ilium? >Also, regardless of wehter we use femur or ilium, which concentration of >EDTA is optimum for decal? > >Missy >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Thu Nov 2 13:57:42 2006 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Thu Nov 2 13:57:51 2006 Subject: [Histonet] Re:Formalin Fixation & IHC Message-ID: <000501c6feb9$28b31e30$0201a8c0@carlba65530bda> For paraffin wax sections requiring Heat-induced antigen retrieval/unmasking, it is not often that one reads such pragmatically accurate, apposite and accessible statements/advice such as Brian's............ My only problem with it is....... I wish I could have stated it so well! The Researchers who carry out Immuno on "briefly" Formalin-fixed cryo/vibratome tissues/sections and do not need any Ag retrieval/unmasking to get good results should also gain knowledge from Brian's words of wisdom. For them, I would add that when you get a poor result in such tissue prep. sytems, try pwax! An example of many such situations: In my Research Centre, a PhD student has struggled to get good results for astrocyte subset localisation, using "PFA"-fixed, perfused cryo/vibratome sections with anti NG2. This went on for a year and then they came to me, for advice. Once we fixed her specimens in good ol' "10% Formalin" for a week, pwax-processed them and carried out Immuno-DAB/Fluorochrome on the AR sections..beautiful results! A year wasted!! "Sure, "horses for courses".. Great respect, Brian. If I may, I will add the guts of your post to my SOPs. Best wishes Carl From dharclerode <@t> cytoritx.com Thu Nov 2 14:16:07 2006 From: dharclerode <@t> cytoritx.com (Donna Harclerode) Date: Thu Nov 2 14:14:36 2006 Subject: [Histonet] San Diego Chapter of Histotechnology is hosting 2 workshops Nov 11 Message-ID: <3DE0F644E093DF4BAE80C254176696A519B2F1@mp-mailserver.macropore.com> Nov 11 2006 the San Diego Chapter of Histotechnology welcomes Dr David Tacha (http://www.biocare.net/) to present Rodent Detection IHC on Mouse and Rat Tissues and Double Stains and Clinical and Research Applications Location: Cytori Therapeutics, 3020 Callan Rd, San Diego Time 11 AM to 4:30PM Lunch will be provided. Cytori has soda and other cold drinks at no cost. There will be no coffee available on Sat. For those that are interested we will be taking Dr. Tacha out to dinner at Rock Bottom in UTC. We have a class limit of 25. The cost of the classes is $15 if you postmark a check before Tuesday Nov 7th. ** If you pay at the door the cost will be $20. This fee includes lunch, but dinner will be separate at the restaurant (cash will be very appreciated for the restaurant) ** To pay for classes please make the checks out and send them to Dusko Trajkovic, Pfizer, 10646 Science Center Drive, San Diego, CA 92121 If anyone not on the San Diego Histo yahoo group would like to attend or more information please contact me at dharclerode@cytoritx.com. Thanks Donna Donna Harclerode, HT, (ASCP), HTL, QIHC Scientist / Immunohistochemistry Cytori Therapeutics 3020 Callan Rd. San Diego, CA 92121 858-458-0900 ext 5416 fax 858 200-0945 dharclerode@cytoritx.com From katri <@t> cogeco.ca Thu Nov 2 16:16:18 2006 From: katri <@t> cogeco.ca (Katri Tuomala) Date: Thu Nov 2 16:16:22 2006 Subject: [Histonet] pH of EDTA References: Message-ID: <000a01c6fecc$854a9980$6a9a9618@Katri> Hi Donna, We use in-house EDTA buffer for retrieving some antigens. We have noticed, that the pH decreases as the buffer gets older. We make a litre at a time and use it within the week. It seems this buffer is not stable and maybe pH needs to be checked and adjusted each time you use it. Maybe you can adjust the pH after diluting it for use, with very dilute sodium hydroxide. I find EDTA buffer bothersome, if your detection system is avidin based, since it brings out the endogenenous biotin in many tissues. We end up doing a biotin block on all the EDTA retrieved tissues. Good luck! Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "donna rossi" To: Sent: Thursday, November 02, 2006 5:50 AM Subject: [Histonet] pH of EDTA > > We are having a problem with EDTA pH 8.0 from LabVision. When we take > the pH of the concentrate it is 8.O. When we dilute it as recommended > it changes to 6.0. Lab Vision replaced our bottles but the new > bottles also come out to 6.0 when diluted. We took the pH of the > Distilled water here and got 7.1. What could be the problem.? Do we > adjust the distilled water to a higher pH? How do we do that? Do we > then need to validate all of our antibodies that use EDTA using the" > new" EDTA? We have also had this problem with Zymed EDTA but not as > radical a difference as the Labvision. It was suggested that we > switch to ready-made EDTA but we have a quantity of this and do not > have the room to store multiple big bottles of this retrieval buffer. > Can someone give me a suggestion? Thanks, Donna, SRHS > _________________________________________________________________ > > [1]Use your PC to make calls at very low rates > References > > 1. http://g.msn.com/8HMAENUS/2749??PS=47575 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From peptolab <@t> hamptons.com Thu Nov 2 17:24:38 2006 From: peptolab <@t> hamptons.com (Jeff Silverman) Date: Thu Nov 2 17:24:43 2006 Subject: [Histonet] Job Opening Message-ID: <000001c6fed6$10ad5810$6401a8c0@jeffrey028c8d9> We have an immediate full time opening for an experienced histology technician working Tuesday-Saturday starting at 6 AM Tues-Friday and whenever you want on Saturday. The job is primarily embedding, cutting and staining and coverslipping- all specials and immuno's are automated on the Artisan and we have a Leica stainer. Formalin free lab using Histochoice and limonene in the stainers. Anywhere from 25 up to 125 bocks per day. No cytology- , no autopsies, just cell blocks. The two pathologists are wonderful and we are a fun-loving easy going group. It is a union position (1199) in a 495 bed hospital in Bay Shore, New York. Fax resumes to 631 968 3689, reply this email, or phone 631 968 3292. Jeff Silverman HT HTL QIHC (ASCP) Pathologists' Assistant Southside Hospital North Shore Long Island Jewish Health System 301 East Main Street Bay Shore, New York 11706 From dfinkelstein <@t> mhri.edu.au Thu Nov 2 17:35:54 2006 From: dfinkelstein <@t> mhri.edu.au (David Finkelstein) Date: Thu Nov 2 17:37:01 2006 Subject: [Histonet] Formalin Fixation & IHC Message-ID: <001301c6fed7$a449edd0$0200a8c0@davidfink> Hi Brian and Helen, I agree with Brian to a limited extent. The range of antibodies that are used in clinical diagnosis have been selected to work used on well fixed antigens and tissues. I used them when available. However if you are developing an IH reaction with a new antibody or using frozen sections the antigens in the tissue can be very sensitive to the amount of fixation. For example: I have one very tricky antigen the Dopamine transporter in the brain. If you do a simple test with 4 sections: 1) no fixation; 2) 10 min fixation, 3) 4 hour fixation or 4) overnight fixation. You will be able to visualize the reaction only on the non fixed and lightly fixed tissue. I do no clinical work only research work and use different types and levels of fixation for each antibody! The answer is; 1) the amount of fixation depends on how the antigen reacts to fixation and then if the antibody will recognize the fixed form of the epitope on the antigen or not. 2) copy an established method for that antibody. Usually get them from a friend, the methods in specification sheets that come with the AB only work sometime. Best of Luck Regards David Assoc. Professor David Finkelstein, The Mental Health Research Institute of Victoria, 155 Oak Street, Parkville, Victoria 3052 AUSTRALIA dfinkelstein@mhri.edu.au ------------------------------ Message: 6 Date: Wed, 1 Nov 2006 15:55:03 -0500 From: "Bryan Hewlett" Subject: Re: [Histonet] Formalin Fixation & IHC To: , "Helen E Johnson" Message-ID: <007401c6fdf8$013adf30$6500a8c0@mainbox> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Hi Helen, "Overfixation" was a term used by early histologists to describe the unexpected and excessive hardening and shrinkage of tissues which occurred following lengthy exposure to certain fixatives. It gave rise to the concept of tolerant and intolerant fixatives. Heidenhain's SUSA and Zenker's fluid are examples of intolerant fixatives and textbooks usually suggest that fixation times should not exceed 24 hours. On the other hand, an aqueous solution of formaldehyde is the classic example of a tolerant fixative, tissues may be left in it for extended periods of time, with no excessive hardening or shrinkage. So-called 'overfixation', as applied to fixation of tissues in aqueous solutions of formaldehyde for IHC, is a total misnomer. Simply put, it never occurs in any clinically relevant time frame (months!!!). Formaldehyde fixes by formation of hydroxymethyl adducts on reactive side chains of proteins. Once sufficient adducts are formed, they slowly cross-link to stabilize the proteins in a gel-like formation. At room temperature, it takes approximately 24 hours for maximal binding of formaldehyde to occur and hence all the adducts to form. These initial adducts, and any initial cross-links formed, are unstable and easily reversed. For tissues fixed for 24 hours, the cross-links are largely reversed by washing ( in water or 70% EtOH) after a few hours. It probably takes at least 5-7 days for most of the cross-links to form, and a small amount of cross-linking still continues over time. Even after fixation for 7 days or more, the cross-links can still be reversed by prolonged washing. That's great for IHC, since cross-links can be reversed by HIER (which is just washing at elevated temperatures!). We have tested over 300 antibodies, using human tissues fixed in NBF for times ranging from 2 hours to 3 months ( some out to 1 year). In no case were we unable to demonstrate the antigen by IHC in tissues fixed for 24 hours or longer. In contrast, many antigens (particularly surface proteins) failed to be demonstrated in the same tissues fixed for less than 24 hours!!! Don't worry about so-called 'overfixation', there is NO SUCH THING. Do worry about underfixation, it can be devastating to IHC. My advice, leave the tissues in formaldehyde for up to 90 days and then if you must, transfer to 70% EtOH for further storage. Regards, Bryan ----- Original Message ----- From: "Helen E Johnson" To: Sent: Wednesday, November 01, 2006 11:38 AM Subject: [Histonet] Formalin Fixation & IHC > > Hi Histonetters, > In order to avoid overfixation of specimens in Formalin when > performing IHC , specimens are usually transferred to 70% EtOH at 24 hrs. > Is there any adverse effects of antigen damage in long term storage in > EtOH? Will specimens get hard if kept long in EtOH? > Helen > Johnson (hej01@health.state.ny.us) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lisab <@t> hoho.org Fri Nov 3 07:27:19 2006 From: lisab <@t> hoho.org (Lisa Brenner) Date: Fri Nov 3 07:27:46 2006 Subject: [Histonet] Leica coverslipper again and does anyone like the Sakura stainer? Message-ID: Hello Everyone, So far I've been asking about the Leica stainer and coverslipper. Does anyone have the Sakura stainer with the attached coverslipper? What are your likes/dislikes with this system? Any advantages file vs. glass coverslips? Back to Leica....does anyone run both H&E and cytology slides, either non-gyn or paps at the same time on the Leica ST5020? Does it delay any of the slides coming out? There has been concern by our Cytotechs that with the Leica glass coverslipper slides have to be dried prior to running on the cytec imager. Does anyone know about this? How long do you dry them? Any problems with cornflaking? Thanks for all your help. It so nice to hear from people who actually use all this equipment. Lisa Brenner HTL (ASCP) Histology Technical Consultant Holland Hospital phone: (616)394-3184 lisab@hoho.org Confidentiality Notice: The information contained in this e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information, or Protected Health Information as such term is defined under the Health Insurance Portability and Accountability Act of 1996 (HIPAA). Any unauthorized review, use, disclosure, copying or distribution is prohibited and may be unlawful. If you believe you have received this e-mail in error, please contact the sender by reply e-mail and delete all copies of the original message, including attachments. From SDrew <@t> uwhealth.org Fri Nov 3 07:28:00 2006 From: SDrew <@t> uwhealth.org (Drew Sally A.) Date: Fri Nov 3 07:28:19 2006 Subject: [Histonet] BAF 47 antibody staining Message-ID: Good afternoon! We have a neuropathologist wanting to know where we can send some slides to get BAF 47 (INI-1 gene product) immunostaining done. I guess this is for an atypical teratoid/rhabdoid tumor? If someone out there does this immunostain, please contact me with the information on how to send the slides and how much the charge will be. Thank you! Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 (608)265-6596 From Nathaniel.Nauss <@t> anmedhealth.org Fri Nov 3 08:59:15 2006 From: Nathaniel.Nauss <@t> anmedhealth.org (Nathaniel Nauss) Date: Fri Nov 3 08:59:50 2006 Subject: [Histonet] Job opening Message-ID: Good Morning, We have a job opening for a full time histotech. AnMed Health System is located in the upstate of South Carolina in Anderson, SC. Any interested techs can submit an application on www.anmed.com, it is listed as a histotechnologist 1. Thank you Nathaniel Nauss From Zsuzsa.Penke-Verdier <@t> ibaic.u-psud.fr Fri Nov 3 09:18:42 2006 From: Zsuzsa.Penke-Verdier <@t> ibaic.u-psud.fr (Zsuzsa Penke-Verdier) Date: Fri Nov 3 09:22:10 2006 Subject: [Histonet] embedding multiple snap frozen brains for cryosectioning Message-ID: Hello Netters, For an in situ hybridation experiment, I need to cut snap frozen mouse brain tissue with a cryostat and put several sections (minimum 7, each from a different brain) on the same slide. Because mRNA is rapidly degraded if cutting takes too long, I cannot afford to cut each of the 7 brains individually. This is why I need to make a composite block out of several brains. However, making such a block is problematic for 2 reasons?: 1. It is important that each brain section should be in the same relative plane (the same anteroposterior coordinate). Does anyone have an idea how I could achieve this?? 2. How could I avoid thawing of brains occasioned by adding freezing medium?? Any suggestion would be welcome. Thanks in advance for your help?! Zsuzsa Penke-Verdier Post doc researcher Laboratory of the Neurobiology of Learning, Memory and Communication NAMC, CNRS UMR 8620, B?t 446 University Paris-Sud 91405 Orsay Cedex, FRANCE Tel:? 33 (0)1 69 15 49 96 Fax: 33 (0)1 69 15 77 26 Zsuzsa.Penke-Verdier@ibaic.u-psud.fr http://h0.web.u-psud.fr/namc/ From petepath <@t> yahoo.com Fri Nov 3 12:05:49 2006 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Fri Nov 3 12:06:01 2006 Subject: [Histonet] embedding multiple snap frozen brains for cryosectioning Message-ID: <20061103180549.6051.qmail@web30402.mail.mud.yahoo.com> Dear Zsuzsa, You can do embed multilpe pieced in a single plane with ease using my embedding well bars. You can wet the snap frozen tissue with a touch of embedding medium and freeze the pieces into place flat on the well floor. If you use cold embedding medium to complete the block you will not thaw your snap frozen tissue. Please visit http://pathologyinnovations.com/index.html to learn more about my Precision Cryoembedding S`ystem. Stephen Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com From PIXLEYSK <@t> UCMAIL.UC.EDU Fri Nov 3 12:49:10 2006 From: PIXLEYSK <@t> UCMAIL.UC.EDU (Pixley, Sarah (pixleysk)) Date: Fri Nov 3 12:48:27 2006 Subject: [Histonet] RE: embedding multiple brains Message-ID: Dear Zsuzsa: We cryosection brains also and this way might work for your needs. We cut the unfrozen brains (after sucrose) down into sections that include the area we are interested. We make the most posterior slice as close as possible to a true vertical plane, so that when we set the brains down on that, they will be flat and we will be cutting as close as possible to true coronal sections. Then we lay the brain down flat in a plastic weigh boat (Fisher Sci. has medium and large ones that might hold several brains at once). Then we fill the weigh boat with OCT compound and mark the outside edges of the weigh boat with the specimen number, mark out the dimensions of the brain and put D and V for dorsal/ventral. Then we sit that weigh boat flat onto a flat piece of dry ice. It takes about 15 mins for the whole thing to freeze. As it freezes, it moves down into the dry ice block. When it is done, it is transferred to the cryostat and we let it equilibrate for at least 15 mins. Sometimes, we have to wait, so we put the frozen block in the weigh boat into a Ziploc bag to keep humidity constant and put in the freezer. After freezing, we also mark the OCT with important numbers and dimensions. When ready to cut, we pop it out of the weigh boat and use a razor blade to cut down the outside OCT to a manageable size rectangular block. We use a small amount of Oct to attach the block to the cryostat chuck and start sectioning. If the top remained flat during freezing, you can use it to get the specimen cutting angle correct and you should be set to go once you get to the tissue. Sometimes we have to fine tune once getting to the tissue. I could envision that you could put several mouse brains in one of the medium or large size weigh boats, or even make home-made boats out of aluminum foil. However, you will be limited by the size of the cryostat chuck and by how well you are able to cut such a large block. Good luck, hope this helps. Sarah Pixley Ohio From gcallis <@t> montana.edu Fri Nov 3 14:05:05 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Nov 3 14:05:16 2006 Subject: Very long reply about EDTA RE: [Histonet] rat myelofibrosis questions In-Reply-To: References: <398f02c20611020837m95c7738m875101c605050f4@mail.gmail.com> <6.0.0.22.1.20061102103919.01b71188@gemini.msu.montana.edu> Message-ID: <6.0.0.22.1.20061103110206.01b2a738@gemini.msu.montana.edu> Barry and fellow Histonetters, This will be a long reply on EDTA decalcification - delete if not interested. Barry, you were correct in your thinking, and not confused. I feel much of the confusion that arises about EDTA decalcification comes from several things. One is not only adjusting the pH upwards but actually changing the EDTA molecule in chemical terms to make decalcification with this molecule work. 1. It helps to understand how EDTA chelation functions to decalcify bone or chelate calcium. EDTA binds to calcium as a function of pH (Harris DE, Quantitative Chemical Analysis, Ch: EDTA Titrations, p271-289, 1982 and the molecule is made up of tetracarboxylic acid (COOH) and 2 amine (NH) groups that are fully protonated at an acidic, LOW pH so that decalcification cannot occur. As one progressively raises the pH (using sodium hydroxide) the protons dissociate from these groups, known as unprotonation, and decalcification become possible. Above pH 10, these groups are completely unprotonated and this form of EDTA binds calcium the strongest. This also makes decalcification proceed at a faster rate. Unfortunately, but this high pH can be damaging to alkaline sensitive protein linkages. Consequently, if you try to use EDTA at pH 3, decalcification is not occuring in fact, it only begins to decalcify very slowly around pH 4. As you continue to raise the pH to 7, decalcification is faster than at pH 4, and at pH 8, the EDTA is even more efficient. However pH 8 is approaching too alkaline for those sensitive protein linkages. This was discussed in JOH, March 1998 Review of Decalcification, Callis and Sterchi. EDTA formulations and EDTA with sodium salts ARE more soluble as the number of sodiums increase. Disodium salt of EDTA is less soluble than tetrasodium salt of EDTA but it is important to note that their pH's are different too. 2. EDTA powders (please note plural here) used for decalcification can be several EDTA formulations a. EDTA (edetic acid) has formula weight of 292, is soluble in water at approx 10%, a concentration that is useful for decalcification. To get this EDTA into solution at this high concentration, heat and adding sodium hydroxide is used which also contributes to helps "unprotonating" the molecule. Dissolved in water, this EDTA probably has acidic pH around 4 or so before adding the sodium hydroxide (your memory served you well! ) b. EDTA disodium has a formula weight 372. A 5% solution has pH 4 to 5 at RT. Once again is soluble in water up to 10% concentration, but people can experience difficulty making up solution until sodium hydroxide is added which adjusts the pH to 7 or so. c. EDTA tetrasodium salt has a formula weight of 380 and is highly soluble in water (14% of more) with a pH of 9.5 - 12. This molecule of EDTA is fully unprotonated, but the high pH is not good for the sensitive protein linkages. It also will decalcify faster at this high pH, but adjusting the pH DOWN with acetic acid to pH 7.4 or a pH needed for enzyme histochemical work is advisable. We have actually decalcified at pH 7.6, which is the pH of our TRIS buffered saline, and people have good immunostaining results. I have seen pH 7.4 as being ideal rather than pushing it to 7.6. When doing enzyme histochemical staining, we stay within a specific, narrow pH range needed for the enzyme. That pH could be even lower than 7. In 1997, Diane Sterchi and I embarked on a decalcification publication. She did all the bone decalcification testing including using 10% EDTA to see the effects of these different pH's (3.2, 7, 10.3) for speed and success with EDTA decalcification. I guess you could call it testing the function of pH of EDTA for calcium binding, and her results were consistent with that dependence on pH. All bones were the same size, from the same animal and she used FAXITRON X-ray to determine endpoint. The results were: EDTA pH 3.2 - the bone never decalcified! EDTA, pH 7.0 - bone decalcified in 40 days EDTA, pH 10.3 - bone decalcified in 16 days This was an eye opener and set standards here for using EDTA decalcification. Now we pay attention to which EDTA is used, it's solubility, original pH. Alll this is found in a Merck Index or some chemical catalog (Aldrich). We then proceed with either using sodium hydroxide to unprotonate the molecule and adjust to a higher pH (7 - 7.4) for faster decalcification without damaging alkaline sensitive protein linkages. OR our favorite, highly soluble tetrasodium EDTA at 14% to put more molecules into solution and available for chelating the calcium with simple pH adjustment with glacial acetic acid. We never used hydrochloric acid for this purpose and followed a Webb Jee publication method using the acetic acid, a decision to follow a bone expert's good results. I have never tried to put EDTA disodium into solution at 14% concentration, but it may work by adding the sodium hydroxide pellets. It sometimes is a guessing game when publications do not tell you the exact formula weight nor nomenclature of EDTA, but I guess one could assume it is usually disodium or the Edetic acid (EDTA alone). So much for exact scientific reporting! I have to thank my physical chemist spouse for lectures on unprotonating the EDTA molecule, complete with charts from a textbook, and no tuition - his fee was h'ors deours (mispelled that one!) dinner, and clean clothes and house for a lifetime. Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 12:04 PM 11/2/2006, you wrote: >I am a little confused so should put my thoughts down. > >EDTA has four possible chelation sites for chelating calcium and other ions. >If you start with EDTA powder and adjust the pH upwards you will first get >mono, then di, then tri and finally tetrasodium EDTA. >EDTA powder by itself if not very soluble until the pH is adjusted. Most >recipes start with the disodium salt. >I forget the actual pHs but memory is that they are around >4.6, 6.5, 8.5 and 11 respectively (forgive me if incorrect but I am not >at my office computer). >Whenever you adjust the pH you alter the EDTA salts that are produced. >So that at pH 7.0 or so you have a micture of disodium and trisodium. >Barry From safety.trainorlab <@t> mts.net Fri Nov 3 15:27:35 2006 From: safety.trainorlab <@t> mts.net (SAFETY TRAINORLAB) Date: Fri Nov 3 15:43:56 2006 Subject: [Histonet] Help with Fix-all Message-ID: <001301c6ff8e$e160e360$19c809c0@tlabs> Hi, Has anyone had any experience with Fix-All Fixative from Surgipath? We are experiencing some fixation problems, mainly with cervical biopsies. From nienhuis <@t> ucla.edu Fri Nov 3 16:09:23 2006 From: nienhuis <@t> ucla.edu (nienhuis@ucla.edu) Date: Fri Nov 3 16:09:30 2006 Subject: [Histonet] Old Vibratome parts? In-Reply-To: <001301c6ff8e$e160e360$19c809c0@tlabs> References: <001301c6ff8e$e160e360$19c809c0@tlabs> Message-ID: <20061103140923.32y9xsmnqlwcscwg@mail.ucla.edu> Does anyone have a manual or know of a source of parts/accessories for an old Oxford Model G Vibratome? Our old one is missing a few bits and the company seems to have been bought out by another outfit. Bob Nienhuis nienhuis@ucla.edu From sohail_e <@t> yahoo.com Sat Nov 4 12:15:00 2006 From: sohail_e <@t> yahoo.com (sohail ejaz) Date: Sat Nov 4 12:15:07 2006 Subject: [Histonet] Toluene and Flouresence Staining Message-ID: <20061104181501.1298.qmail@web39502.mail.mud.yahoo.com> I am studying tracheal vasculature by using vWF as blood vessel marker. After staining i am using toluene to clear the tissue, but after using that flouresence goes down..................?? Can anyone guide me for to slove this issue. If some one have a better protocol for tracheal whole mounnt staining, I would love to try for that. Regard Dr. Rudiger Tom University of Connecticut, Storrs Connecticut --------------------------------- Low, Low, Low Rates! Check out Yahoo! Messenger's cheap PC-to-Phone call rates. From dfinkelstein <@t> mhri.edu.au Sat Nov 4 19:10:30 2006 From: dfinkelstein <@t> mhri.edu.au (David Finkelstein) Date: Sat Nov 4 19:11:01 2006 Subject: [Histonet] embedding multiple snap frozen brains Message-ID: <001701c70077$30654580$0300a8c0@davidfink> Dear Stephen Peters and Sarah Pixley, I am very interested in your methods on freezing brains. My question is I have never been able to cut brains that have been covered with OCT. How do you do it.??? Suggestions please? Most histologists I know leave the bit of brain that they want sticking out of the OCT. The OCT is a different hardness to the brain. If cutting nonfixed brain at -14oc the OCT is too soft and fixed brains at -25o C the OCT is to hard. It is even harder to get the hardness\temperature right for foetal tissue. The solution has been leave it sticking out of the OCT. David Assoc. Professor David Finkelstein, The Mental Health Research Institute of Victoria, 155 Oak Street, Parkville, Victoria 3052 AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 36, Issue 4 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. embedding multiple snap frozen brains for cryosectioning (Stephen Peters M.D.) 2. RE: embedding multiple brains (Pixley, Sarah (pixleysk)) 3. Very long reply about EDTA RE: [Histonet] rat myelofibrosis questions (Gayle Callis) 4. Help with Fix-all (SAFETY TRAINORLAB) 5. Old Vibratome parts? (nienhuis@ucla.edu) ---------------------------------------------------------------------- Message: 1 Date: Fri, 3 Nov 2006 10:05:49 -0800 (PST) From: "Stephen Peters M.D." Subject: [Histonet] embedding multiple snap frozen brains for cryosectioning To: Histonet@lists.utsouthwestern.edu Message-ID: <20061103180549.6051.qmail@web30402.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Dear Zsuzsa, You can do embed multilpe pieced in a single plane with ease using my embedding well bars. You can wet the snap frozen tissue with a touch of embedding medium and freeze the pieces into place flat on the well floor. If you use cold embedding medium t o complete the block you will not thaw your snap frozen tissue. Please visit http://pathologyinnovations.com/index.html to learn more about my Precision Cryoembedding S`ystem. Stephen Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com ------------------------------ Message: 2 Date: Fri, 3 Nov 2006 13:49:10 -0500 From: "Pixley, Sarah \(pixleysk\)" Subject: [Histonet] RE: embedding multiple brains To: Message-ID: Content-Type: text/plain; charset="us-ascii" Dear Zsuzsa: We cryosection brains also and this way might work for your needs. We cut the unfrozen brains (after sucrose) down into sections that include the area we are interested. We make the most posterior slice as close as possible to a true vertical plane, so that when we set the brains down on that, they will be flat and we will be cutting as close as possible to true coronal sections. Then we lay the brain down flat in a plastic weigh boat (Fisher Sci. has medium and large ones that might hold several brains at once). Then we fill the weigh boat with OCT compound and mark the outside edges of the weigh boat with the specimen number, mark out the dimensions of the brain and put D and V for dorsal/ventral. Then we sit that weigh boat flat onto a flat piece of dry ice. It takes about 15 mins for the whole thing to freeze. As it freezes, it moves down into the dry ice block. When it is done, it is transferred to the cryostat and we let it equilibrate for at least 15 mins. Sometimes, we have to wait, so we put the frozen block in the weigh boat into a Ziploc bag to keep humidity constant and put in the freezer. After freezing, we also mark the OCT with important numbers and dimensions. When ready to cut, we pop it out of the weigh boat and use a razor blade to cut down the outside OCT to a manageable size rectangular block. We use a small amount of Oct to attach the block to the cryostat chuck and start sectioning. If the top remained flat during freezing, you can use it to get the specimen cutting angle correct and you should be set to go once you get to the tissue. Sometimes we have to fine tune once getting to the tissue. I could envision that you could put several mouse brains in one of the medium or large size weigh boats, or even make home-made boats out of aluminum foil. However, you will be limited by the size of the cryostat chuck and by how well you are able to cut such a large block. Good luck, hope this helps. Sarah Pixley Ohio From jkiernan <@t> uwo.ca Sat Nov 4 22:52:51 2006 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Sat Nov 4 22:53:18 2006 Subject: [Histonet] Toluene and Flouresence Staining References: <20061104181501.1298.qmail@web39502.mail.mud.yahoo.com> Message-ID: <454D6E23.E11CA1C1@uwo.ca> Send a detailed account of your technique. You will many very helpful replies. __________________ sohail ejaz wrote: > > I am studying tracheal vasculature by using vWF as blood vessel marker. > After staining i am using toluene to clear the tissue, but after using that flouresence goes down..................?? > > Can anyone guide me for to slove this issue. > > If some one have a better protocol for tracheal whole mounnt staining, I would love to try for that. > > Regard > > Dr. Rudiger Tom > University of Connecticut, Storrs > Connecticut > > > --------------------------------- > Low, Low, Low Rates! Check out Yahoo! Messenger's cheap PC-to-Phone call rates. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Pathrm35 <@t> adelphia.net Sun Nov 5 16:25:26 2006 From: Pathrm35 <@t> adelphia.net (Ron Martin) Date: Sun Nov 5 16:25:21 2006 Subject: [Histonet] unsubscribe Message-ID: <000001c70129$4af733b0$2ea2ac45@D6WRV2B1> From jkiernan <@t> uwo.ca Sun Nov 5 22:46:57 2006 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Sun Nov 5 22:48:08 2006 Subject: [Histonet] marker for brain inflammation References: <3665A19D-43E0-4583-A345-93EE8AE4C30D@bidmc.harvard.edu> Message-ID: <454EBE41.893DE1FA@uwo.ca> Inflammation is defined as the response of living tissue to injury, characterized by vasodilation, swelling (increased vascular permeability) and pain. The second of these cardinal signs is demonstrable by immunostaining for plasma proteins. In the normal central nervous system (except in the choroid plexuses, the 5 circumventricular organs, and at the lamina cribrosa of the optic nerve head), plasma proteins occur only within blood vessels. If you see them outside vessels there has been a failure of the blood-brain barrier, which could well be due to inflammation. There is an artifact to avoid. Postmortem delay can allow plasma proteins to diffuse out of the vasculature and into some neurons and neuroglial cells. I've seen this in human brain, and the artifact is well documented also for animals. See: Mori S, Sternberger N h, Herman NM, Sternberger l A (1991) Leakage and neuronal uptake of serum protein in aged and Alzheimer brains: a postmortem phenomenon with antemortem etiology. Laboratory Investigation 64: 345-351. Fabian RH (1992) Poor reliability of immunocytochemical localization of IgG in immersion-fixed tissue from the central nervous system. Journal of Histochemistry and Cytochemistry 40: 987-991. Loberg EM, Torvik A (1992) Plasma proteins in normal neurons: immunohistochemical studies on autopsy material and experimental animals. Acta pathologica et microbiologica scandinavica 100: 431-436. A similar artifact is seen if immunohistochemistry is attempted on unfixed cryostat sections of the rat's brain. Sparrow JR (1980) Immunohistochemical study of the blood-brain barrier. Production of an artifact. Journal of Histochemistry and Cytochemistry 26: 570-572. John Kiernan Anatomy Dept, U.W.O. London, Canada. ______________________ Caroline Bass wrote: > > Hello, > > I'm wondering if someone could suggest a marker or stain that could > help visualize inflammation. I am working on a project where I > inject a virus in the brain to introduce genes in neurons. However, > I want to make sure that the injection itself, or the neuronal > infection is not causing a large degree of inflammation. Could > someone suggest a good way to visualize this? I imagine some sort of > immune marker will do, perhaps mF4/80? > > thanks, > > Caroline > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cwscouten <@t> myneurolab.com Mon Nov 6 09:01:41 2006 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Mon Nov 6 09:01:12 2006 Subject: [Histonet] embedding multiple snap frozen brains Message-ID: <5784D843593D874C93E9BADCB87342AB01307808@tpiserver03.Coretech-holdings.com> Why embed in OCT? Freeze by immersion of the brain, perhaps after cutting a plane through. Mount the frozen brain to a pedestal with a small amount of OCT used as glue around the base, perhaps after pre-refrigeration to cool it. Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Finkelstein Sent: Saturday, November 04, 2006 7:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] embedding multiple snap frozen brains Dear Stephen Peters and Sarah Pixley, I am very interested in your methods on freezing brains. My question is I have never been able to cut brains that have been covered with OCT. How do you do it.??? Suggestions please? Most histologists I know leave the bit of brain that they want sticking out of the OCT. The OCT is a different hardness to the brain. If cutting nonfixed brain at -14oc the OCT is too soft and fixed brains at -25o C the OCT is to hard. It is even harder to get the hardness\temperature right for foetal tissue. The solution has been leave it sticking out of the OCT. David Assoc. Professor David Finkelstein, The Mental Health Research Institute of Victoria, 155 Oak Street, Parkville, Victoria 3052 AUSTRALIA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 36, Issue 4 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. embedding multiple snap frozen brains for cryosectioning (Stephen Peters M.D.) 2. RE: embedding multiple brains (Pixley, Sarah (pixleysk)) 3. Very long reply about EDTA RE: [Histonet] rat myelofibrosis questions (Gayle Callis) 4. Help with Fix-all (SAFETY TRAINORLAB) 5. Old Vibratome parts? (nienhuis@ucla.edu) ---------------------------------------------------------------------- Message: 1 Date: Fri, 3 Nov 2006 10:05:49 -0800 (PST) From: "Stephen Peters M.D." Subject: [Histonet] embedding multiple snap frozen brains for cryosectioning To: Histonet@lists.utsouthwestern.edu Message-ID: <20061103180549.6051.qmail@web30402.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Dear Zsuzsa, You can do embed multilpe pieced in a single plane with ease using my embedding well bars. You can wet the snap frozen tissue with a touch of embedding medium and freeze the pieces into place flat on the well floor. If you use cold embedding medium t o complete the block you will not thaw your snap frozen tissue. Please visit http://pathologyinnovations.com/index.html to learn more about my Precision Cryoembedding S`ystem. Stephen Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com ------------------------------ Message: 2 Date: Fri, 3 Nov 2006 13:49:10 -0500 From: "Pixley, Sarah \(pixleysk\)" Subject: [Histonet] RE: embedding multiple brains To: Message-ID: Content-Type: text/plain; charset="us-ascii" Dear Zsuzsa: We cryosection brains also and this way might work for your needs. We cut the unfrozen brains (after sucrose) down into sections that include the area we are interested. We make the most posterior slice as close as possible to a true vertical plane, so that when we set the brains down on that, they will be flat and we will be cutting as close as possible to true coronal sections. Then we lay the brain down flat in a plastic weigh boat (Fisher Sci. has medium and large ones that might hold several brains at once). Then we fill the weigh boat with OCT compound and mark the outside edges of the weigh boat with the specimen number, mark out the dimensions of the brain and put D and V for dorsal/ventral. Then we sit that weigh boat flat onto a flat piece of dry ice. It takes about 15 mins for the whole thing to freeze. As it freezes, it moves down into the dry ice block. When it is done, it is transferred to the cryostat and we let it equilibrate for at least 15 mins. Sometimes, we have to wait, so we put the frozen block in the weigh boat into a Ziploc bag to keep humidity constant and put in the freezer. After freezing, we also mark the OCT with important numbers and dimensions. When ready to cut, we pop it out of the weigh boat and use a razor blade to cut down the outside OCT to a manageable size rectangular block. We use a small amount of Oct to attach the block to the cryostat chuck and start sectioning. If the top remained flat during freezing, you can use it to get the specimen cutting angle correct and you should be set to go once you get to the tissue. Sometimes we have to fine tune once getting to the tissue. I could envision that you could put several mouse brains in one of the medium or large size weigh boats, or even make home-made boats out of aluminum foil. However, you will be limited by the size of the cryostat chuck and by how well you are able to cut such a large block. Good luck, hope this helps. Sarah Pixley Ohio _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cwscouten <@t> myneurolab.com Mon Nov 6 09:08:28 2006 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Mon Nov 6 09:07:56 2006 Subject: [Histonet] Old Vibratome parts? Message-ID: <5784D843593D874C93E9BADCB87342AB01307809@tpiserver03.Coretech-holdings.com> This instrument has always been manufactured in St. Louis by Technical Products International, now called Vibratome Company. It was bought out, but there is still involvement of the original founders. More to the point, parts for the original model, now called the classic, are still available. I have copied Christina at Vibratome company. Contact her for what you need. Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of nienhuis@ucla.edu Sent: Friday, November 03, 2006 4:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Old Vibratome parts? Does anyone have a manual or know of a source of parts/accessories for an old Oxford Model G Vibratome? Our old one is missing a few bits and the company seems to have been bought out by another outfit. Bob Nienhuis nienhuis@ucla.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Mon Nov 6 09:16:24 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Nov 6 09:16:30 2006 Subject: [Histonet] Toluene and Flouresence Staining In-Reply-To: <20061104181501.1298.qmail@web39502.mail.mud.yahoo.com> References: <20061104181501.1298.qmail@web39502.mail.mud.yahoo.com> Message-ID: <6.0.0.22.1.20061106081354.01b582e8@gemini.msu.montana.edu> Depending on the fluorphore you are using, you should mount the coverslip with an antifade mounting media immediately after staining is completed. Do not dehydrate or clear through the same solvents as you would use for an H&E stain. A good mounting media is Prolong gold antifade from Molecular Probes, but there are others on the market from Vector and others. For a good discussion on mounting medias used for this purpose, go to IHC world, and into the fluorescence heading.s. At 11:15 AM 11/4/2006, you wrote: >I am studying tracheal vasculature by using vWF as blood vessel marker. > After staining i am using toluene to clear the tissue, but after using > that flouresence goes down..................?? > > Can anyone guide me for to slove this issue. > > If some one have a better protocol for tracheal whole mounnt staining, > I would love to try for that. > > Regard > > Dr. Rudiger Tom > University of Connecticut, Storrs > Connecticut > > >--------------------------------- >Low, Low, Low Rates! Check out Yahoo! Messenger's cheap PC-to-Phone call >rates. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From LBUSTAMANTE <@t> cvm.tamu.edu Mon Nov 6 11:18:47 2006 From: LBUSTAMANTE <@t> cvm.tamu.edu (Lin Bustamante) Date: Mon Nov 6 11:19:16 2006 Subject: [Histonet] Thin Paraffin Sections Message-ID: <454F1A17020000B90000973D@vetmed1.CVM.TAMU.EDU> Hi Histologists. Someone came by our lab and asked about very thin sections in paraffin, 0.5 um to be exact. Has anyone any experience or information on this. Thank you very much. Lin S. Bustamante, B.Sc.; HT(ASCP) Histology Lab Dept. of Veterinary Anatomy and Public Health Texas A&M University College Station, TX 77843-4458 From ian.montgomery <@t> bio.gla.ac.uk Mon Nov 6 11:49:30 2006 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Mon Nov 6 11:49:52 2006 Subject: Fw: [Histonet] Thin Paraffin Sections Message-ID: <008f01c701cb$e97200e0$4724d182@ibls.gla.ac.uk> Lin, If it has to be wax then ester wax would be my choice. 0.5um is really pushing it although I've cut ester wax at 1um in the past. Depending on the application I think resin would be a better choice. Ian. Dr. Ian Montgomery, Histotechnology, IBLS Support Services, Thomson Building, University of Glasgow, Tel:01413398855 Extn: 8511. ----- Original Message ----- From: "Lin Bustamante" To: Sent: Monday, November 06, 2006 5:18 PM Subject: [Histonet] Thin Paraffin Sections > Hi Histologists. > Someone came by our lab and asked about very thin sections in paraffin, > 0.5 um to be exact. > Has anyone any experience or information on this. > Thank you very much. > > Lin S. Bustamante, B.Sc.; HT(ASCP) > Histology Lab > Dept. of Veterinary Anatomy and Public Health > Texas A&M University > College Station, TX 77843-4458 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From PIXLEYSK <@t> UCMAIL.UC.EDU Mon Nov 6 12:12:34 2006 From: PIXLEYSK <@t> UCMAIL.UC.EDU (Pixley, Sarah (pixleysk)) Date: Mon Nov 6 12:11:48 2006 Subject: [Histonet] RE: Histonet Digest, Vol 36, Issue 5 Message-ID: Dear David Finkelstein: I apologize for not thinking harder about your problem before I wrote. You have snap frozen brains, while I am working with paraformaldehyde fixed brains. Big difference. I am able to take the room temperature (or fridge temp) brains and coat them with OCT and then freeze both OCT and brains together. This works fine and cuts fine. We don't really have too many problems with the brains coming out of the OCT. Occasionally they do come out, but not often. So this may perhaps explain the differences. Otherwise, other details are that we cut at either 14 um or 20 um, and we thaw mount onto slides. We cut at -20 degrees C. Another suggestion is to use another embedding matrix. I like the Shandon Thermo M1 embedding matrix because it doesn't curl as badly as OCT. But right now we are using OCT because when you make a big block of it, OCT is easier to cut than the M1. Sarah Pixley Message: 2 Date: Sun, 5 Nov 2006 12:10:30 +1100 From: "David Finkelstein" Subject: [Histonet] embedding multiple snap frozen brains To: Message-ID: <001701c70077$30654580$0300a8c0@davidfink> Content-Type: text/plain; charset="us-ascii" Dear Stephen Peters and Sarah Pixley, I am very interested in your methods on freezing brains. My question is I have never been able to cut brains that have been covered with OCT. How do you do it.??? Suggestions please? Most histologists I know leave the bit of brain that they want sticking out of the OCT. The OCT is a different hardness to the brain. If cutting nonfixed brain at -14oc the OCT is too soft and fixed brains at -25o C the OCT is to hard. It is even harder to get the hardness\temperature right for foetal tissue. The solution has been leave it sticking out of the OCT. David Assoc. Professor David Finkelstein, The Mental Health Research Institute of Victoria, 155 Oak Street, Parkville, Victoria 3052 AUSTRALIA From aneeshdhiman <@t> gmail.com Mon Nov 6 12:15:52 2006 From: aneeshdhiman <@t> gmail.com (aneesh Dhiman) Date: Mon Nov 6 12:16:06 2006 Subject: [Histonet] TMA Message-ID: <69ac8f280611061015y75bbd65eu14c9345925b503d4@mail.gmail.com> Hi Everyone Can someone be kind and describe me in short the TMA procedure or recommend a text the book which details the Tissue microarray procedure. Thank you Aneesh From campisi <@t> ipmc.cnrs.fr Mon Nov 6 12:23:09 2006 From: campisi <@t> ipmc.cnrs.fr (campisi@ipmc.cnrs.fr) Date: Mon Nov 6 12:23:50 2006 Subject: [Histonet] help: holes in the spleen... Message-ID: <1162837389.454f7d8d3a1d4@mail.ipmc.cnrs.fr> Hello, I am trying to get confocal images of spleens of Listeria infected mice. Sadly, I have ran into a problem. I get some large holes in the tissue right after cutting the block. These holes appear in areas where the spleen appears almost black on the face of the block.The protocol I am following is the next one:Isolate the spleen, incubate 4 to 6 hrs in Paraformaldehyde 1%, and leave overnight in sucrose 30%. Spleens are included in OCT and frozen on dry ice. All suggestions are welcomed. ---------------------------------------------------------------- This message was sent using IMP, the Internet Messaging Program. From ancillarypath <@t> mac.com Mon Nov 6 12:36:32 2006 From: ancillarypath <@t> mac.com (ancillarypath@mac.com) Date: Mon Nov 6 12:36:52 2006 Subject: [Histonet] Job posting for experienced IHC/FISH techs In-Reply-To: <200611061812.kA6IBnw7023429@mac.com> References: <200611061812.kA6IBnw7023429@mac.com> Message-ID: Dear colleagues, This posting is about the need for full-time experienced techologists (histotechs or medtechs) in IHC +/- FISH. We have a state-of-the-art facility in Miami and we perform high volume and complexity testing including validation of novel antibodies/probes, new automation solutions. We look forward to interviewing senior-level technologists with documented extensive experience in the field. Please respond privately to this email address and submit your CV along with 3 possible references to contact. Please let me know if you have any questions. Best regards, Hadi Yaziji, M.D. President, Ancillary Pathways, LLC. 7000 SW 62nd Ave, Suite PH-C Miami, FL 33143 Phone: 305.740.4440 Cell: 786.266.5725 Fax: 786-513-0175 www.ancillarypath.com From gcallis <@t> montana.edu Mon Nov 6 13:01:50 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Nov 6 13:02:08 2006 Subject: [Histonet] Thin Paraffin Sections In-Reply-To: <454F1A17020000B90000973D@vetmed1.CVM.TAMU.EDU> References: <454F1A17020000B90000973D@vetmed1.CVM.TAMU.EDU> Message-ID: <6.0.0.22.1.20061106115756.01b3ab50@gemini.msu.montana.edu> Lin, 0.5 um is very thin for paraffin medium. The thinnest paraffin section we have cut is 1 um using Accuedge disposable blades, either high or low profile. When we need the thinner 0.5 um sections, we use glycol methacrylate plastic embedding and section with glass knives. If you decide to cut thin paraffin, a harder paraffin often is better - there are several on the market. At 10:18 AM 11/6/2006, you wrote: >Hi Histologists. >Someone came by our lab and asked about very thin sections in paraffin, >0.5 um to be exact. >Has anyone any experience or information on this. >Thank you very much. > >Lin S. Bustamante, B.Sc.; HT(ASCP) >Histology Lab >Dept. of Veterinary Anatomy and Public Health >Texas A&M University >College Station, TX 77843-4458 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From hfedor <@t> jhmi.edu Mon Nov 6 13:04:43 2006 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Mon Nov 6 13:05:23 2006 Subject: [Histonet] TMA In-Reply-To: <69ac8f280611061015y75bbd65eu14c9345925b503d4@mail.gmail.com> References: <69ac8f280611061015y75bbd65eu14c9345925b503d4@mail.gmail.com> Message-ID: <454F40FA.61A1.0088.3@jhmi.edu> Dear Aneesh, here are three websites with everytning there is to know about TMA. http://tmalab.jhmi.edu/?SMSESSION=NO http://www.beecherinstruments.com/ http://www.ihcworld.com/tissuearray.htm best regards, Helen >>> aneesh Dhiman 11/6/2006 1:15 PM >>> Hi Everyone Can someone be kind and describe me in short the TMA procedure or recommend a text the book which details the Tissue microarray procedure. Thank you Aneesh _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Helen L. Fedor B.S. Johns Hopkins University Pathology Department 600 N Wolfe St Marburg Room 406 Baltimore MD 21287 email: hfedor@jhmi.edu Phone: 410 614-1660 Pager: 410 283-3419 WARNING: E-mail sent over the Internet is not secure. Information sent by e-mail may not remain confidential. DISCLAIMER: This e-mail is intended only for the individual to whom it is addressed. It may be used only in accordance with applicable laws. If you received this e-mail by mistake, notify the sender and destroy the e-mail. From amgomez <@t> mail.unomaha.edu Mon Nov 6 13:14:13 2006 From: amgomez <@t> mail.unomaha.edu (Adam M Gomez) Date: Mon Nov 6 13:14:10 2006 Subject: [Histonet] Frozen section problems!! Message-ID: We are wanting to section peripheral gang cryostat. Our current procedure is as follows: Fixation: 1)perfused with PBS/8% Para, ganglia tissue removed < color 10 min. 3)placed in 30% sucrose overnight (or until 4)rinsed in PBS/dH20 5)embedded in OCT 6)frozen rapidly in liquid nitrogen 7)tissue allowed to equilibrate to cryostat temp & sectio cryostat (-18 C) 8)sections hour. 9)Slides are dipped in clearing agent (xylenes) alcohol (95%, 70%, 50%), dH2O, cresyl violet stain, dH2 95%, & xylenes...and coverslipped immediately. The problem we are having is that the tissue looks outstanding after sectioning as long as it remains wet (i.e., when we take a look at tissue d we're losing ce seems that the dehydr tissue is the culprit. We have tr procedure but have yet to find a successf the tissue. If you have any suggestions p problem has been detrimental to our work for the pa Thanks, Adam Gomez Research&nb University of Nebraska at Om Department of Psychology (402) 554-6028 References 1. 3D"mailto:amgomez@mail.unomaha.edu" From mcauliff <@t> umdnj.edu Mon Nov 6 13:19:34 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Mon Nov 6 13:43:52 2006 Subject: [Histonet] Thin Paraffin Sections In-Reply-To: <454F1A17020000B90000973D@vetmed1.CVM.TAMU.EDU> References: <454F1A17020000B90000973D@vetmed1.CVM.TAMU.EDU> Message-ID: <454F8AC6.1060809@umdnj.edu> My advice; before investing a lot of time on this find out if the person really understands what they want. It may be a case of "if 5 micron sections are good then 0.5 micron sections must be 10X as good." It could also be a typo. Years ago a colleague (with a Ph.D.) who should have known better came to me looking for some "NaH", not understanding that the protocol called for NaOH. Geoff Lin Bustamante wrote: >Hi Histologists. >Someone came by our lab and asked about very thin sections in paraffin, >0.5 um to be exact. >Has anyone any experience or information on this. >Thank you very much. > >Lin S. Bustamante, B.Sc.; HT(ASCP) >Histology Lab >Dept. of Veterinary Anatomy and Public Health >Texas A&M University >College Station, TX 77843-4458 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From tissuearray <@t> hotmail.com Mon Nov 6 13:48:26 2006 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Mon Nov 6 13:48:40 2006 Subject: [Histonet] TMA In-Reply-To: <69ac8f280611061015y75bbd65eu14c9345925b503d4@mail.gmail.com> Message-ID: If you go to [1]www.arrayworkshop.com you will find sever aritcles on array construction and other helpful information. Even advanced techniques and new helpful equipment. Cheers, Thom Jensen ______________________________________________________________ From: "aneesh Dhiman" To: histonet@lists.utsouthwestern.edu Subject: [Histonet] TMA Date: Mon, 6 Nov 2006 13:15:52 -0500 MIME-Version: 1.0 Received: from swlx162.swmed.edu ([199.165.152.162]) by bay0-mc2-f2.bay0.hotmail.com with Microsoft SMTPSVC(6.0.3790.2444); Mon, 6 Nov 2006 10:16:33 -0800 Received: from localhost ([127.0.0.1] helo=swlx162.swmed.edu)by swlx162.swmed.edu with esmtp (Exim 4.34)id 1Gh919-0002xH-DN; Mon, 06 Nov 2006 12:16:20 -0600 Received: from [199.242.236.161] (helo=swlx161.swmed.edu)by swlx162.swmed.edu with esmtp (Exim 4.34) id 1Gh90p-0002x1-2Mfor histonet@lists.utsouthwestern.edu; Mon, 06 Nov 2006 12:16:04 -0600 Received: from ug-out-1314.google.com ([66.249.92.170])by swlx161.swmed.edu with esmtp (Exim 4.62)(envelope-from ) id 1Gh90k-0000kN-7pfor histonet@lists.utsouthwestern.edu; Mon, 06 Nov 2006 12:15:59 -0600 Received: by ug-out-1314.google.com with SMTP id k3so816701ugffor ;Mon, 06 Nov 2006 10:15:53 -0800 (PST) Received: by 10.78.117.10 with SMTP id p10mr7018811huc.1162836952398;Mon, 06 Nov 2006 10:15:52 -0800 (PST) Received: by 10.78.194.15 with HTTP; Mon, 6 Nov 2006 10:15:52 -0800 (PST) >Hi Everyone >Can someone be kind and describe me in short the TMA procedure or >recommend >a text the book which details the Tissue microarray procedure. >Thank you >Aneesh >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ [2]All-in-one security and maintenance for your PC. Get a free 90-day trial! References 1. http://www.arrayworkshop.com/ 2. http://g.msn.com/8HMBENUS/2752??PS=47575 From lu_ze <@t> sbcglobal.net Mon Nov 6 13:48:46 2006 From: lu_ze <@t> sbcglobal.net (Ze Lu) Date: Mon Nov 6 13:48:53 2006 Subject: [Histonet] Help on pathology service on mice tissue Message-ID: <065701c701dc$9284d440$0902a8c0@OPTIMUM2> Histonet friends, In one of our current projects, we need pathology report on the spleen, intestine, liver, lymph node from drug treated mice. Dose any one can refer some companies or groups that can provide such service? We will appreciate. Ze ============================ Ze Lu Optimum Therapeutics, LLC From nfournier <@t> sasktel.net Mon Nov 6 23:08:07 2006 From: nfournier <@t> sasktel.net (Neil Fournier) Date: Mon Nov 6 23:08:18 2006 Subject: [Histonet] brain embedding Message-ID: <000601c7022a$b67d85b0$37e02fcf@NEIL> I was wondering if anyone has familiarity with embedding fixed rat brain tissue using agar or agarose for sectioning on a vibrating microtome (Vibratome)? My supervisor feels that it might be worthwhile to consider embedding our tissue using these procedures since it might facilitate sectioning on a vibratome. (We often want to collect sections that could potentially range from 20 to 40 microns depending on experimental procedure). Would someone be able to provide me with a detail procedure and/or protocol for embedding rat brain tissue? Also is there a reason for why one should use agar versus agarose for embedding? And could this embedding procedure potentially interfere with immunostaining? I appreciate your help and any suggestions, Neil From Johan.Kreuger <@t> genpat.uu.se Tue Nov 7 02:09:41 2006 From: Johan.Kreuger <@t> genpat.uu.se (Johan Kreuger) Date: Tue Nov 7 02:09:58 2006 Subject: [Histonet] detection of neomycin resistant cells Message-ID: <6.2.3.4.0.20061107085714.01ef2d70@pop.uu.se> Dear colleagues, We are looking for an antibody to detect murine knockout stem cells that are neomycin resistant. I guess the antibody might be called anti-neo, and be directed against Phosphotransferase 2, but it has been surprisingly difficult to get good information about this. Could you please recommend an antibody that works well for this type of detection. Kind regards, Johan From Susan.Ferrigon <@t> sanofi-aventis.com Tue Nov 7 08:42:31 2006 From: Susan.Ferrigon <@t> sanofi-aventis.com (Susan.Ferrigon@sanofi-aventis.com) Date: Tue Nov 7 08:43:21 2006 Subject: [Histonet] F4/80 Message-ID: <90B6684A9D6DAF468F7A5DC148754E1D1E1869@ALPW31.f2.enterprise> Hi Histonetters I have been able to stain some mouse liver for Kuppfer cells using Serotec's F4/80 and copious amounts of help from their tech support, however I would like to use some immunofluorescence instead of the DAB. At present I am using a Goat anti Rat IgG( Mouse adsorbed) :HRP secondary. Does anyone know of another secondary I could use with a fluorescent marker. I am using FFPE or cryostat sections. Thanks Susan From jessgrocki <@t> yahoo.com Tue Nov 7 09:39:56 2006 From: jessgrocki <@t> yahoo.com (Jessica Piche) Date: Tue Nov 7 09:40:02 2006 Subject: [Histonet] B5 fixative alternatives, and xylene alternatives Message-ID: <20061107153956.15478.qmail@web82007.mail.mud.yahoo.com> Hello, I was just wondering if anyone new of any mercury (B5 fixative) alternatives, and if they will keep the pathologist happy? Also any xylene alternatives that work really well? Thank you, Jessica Piche-Grocki, HT (ASCP) Waterbury Hospital From jessgrocki <@t> yahoo.com Tue Nov 7 09:39:56 2006 From: jessgrocki <@t> yahoo.com (Jessica Piche) Date: Tue Nov 7 09:40:05 2006 Subject: [Histonet] B5 fixative alternatives, and xylene alternatives Message-ID: <20061107153956.15478.qmail@web82007.mail.mud.yahoo.com> Hello, I was just wondering if anyone new of any mercury (B5 fixative) alternatives, and if they will keep the pathologist happy? Also any xylene alternatives that work really well? Thank you, Jessica Piche-Grocki, HT (ASCP) Waterbury Hospital From LINDA.MARGRAF <@t> childrens.com Tue Nov 7 10:21:38 2006 From: LINDA.MARGRAF <@t> childrens.com (LINDA MARGRAF) Date: Tue Nov 7 10:21:58 2006 Subject: [Histonet] Fwd: Bubble artifact for Histonet Message-ID: Histonetters: We are looking for help in identifying a particular artifact that we have seen off and on for the last few years. We call it "bubbling" artifact. The artifact seems to be somewhat random as shown in the pictures provided by the link http://www.histonet.org/site_images_frame.asp (Called bubbles and no bubbles posted by Linda Margraf) (If link doesn't work go to www.Histonet.org and click on "view list serv images"...these pictures are at the top) In one picture, the artifact is clearly visible; in another picture, it is not present at all. These pictures are of a particular GI biopsy and the sections are right next to each other on the slide. Same case, same ribbon, same biopsy, same staining, same processing, same coverslipping...everything is exactly the same except that one has the artifact and the other does not. Has anybody else ever encountered something like this? And if so, were you able to narrow down the cause to something specific? Any help you can offer us would be greatly appreciated Thanks! Michelle L McNeese, HT (ASCP) Lead Tech, Histology Department of Pathology Childrens Medical Center (214) 456-6146 Email: michelle.mcneese@childrens.com From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue Nov 7 10:42:50 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Nov 7 10:42:02 2006 Subject: [Histonet] Fwd: Bubble artifact for Histonet Message-ID: <86ADE4EB583CE64799A9924684A0FBBFAFC8FB@wahtntex2.waht.swest.nhs.uk> I had a fixation over bubbles, once. Get it. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Habits of thinking need not be forever. One of the most significant findings in psychology in the last twenty years is that individuals can choose the way they think. --Martin Seligman This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From tbraud <@t> holyredeemer.com Tue Nov 7 11:28:06 2006 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Tue Nov 7 11:29:50 2006 Subject: [Histonet] cut resistant gloves Message-ID: Hi Histonetters - We recently had a serious cut in the grossing room. Does anyone require their personnel to wear cut-resistant liners? If so, for what duties? Do the pathologists routinely wear them? Any other thoughts or comments on the topic are welcome. Thanks, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supv. Laboratory, Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3689 CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. From jcline <@t> wchsys.org Tue Nov 7 11:38:25 2006 From: jcline <@t> wchsys.org (Joyce Cline) Date: Tue Nov 7 11:38:37 2006 Subject: [Histonet] B5 fixative alternatives, and xylene alternatives In-Reply-To: <20061107153956.15478.qmail@web82007.mail.mud.yahoo.com> Message-ID: <000001c70293$8822d260$1d2a14ac@wchsys.org> Hi, I have used Clear rite 3 from Richard Allen for 15 years or more, I now use Formula 83 from CBG (recycling) and it is almost like Xylene. I use B+ as a B5 replacement, the path's are satisfied with it. B+ comes from BBC Biochemical. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica Piche Sent: Tuesday, November 07, 2006 10:40 AM To: histonet@pathology.swmed.edu; histonet Subject: [Histonet] B5 fixative alternatives, and xylene alternatives Hello, I was just wondering if anyone new of any mercury (B5 fixative) alternatives, and if they will keep the pathologist happy? Also any xylene alternatives that work really well? Thank you, Jessica Piche-Grocki, HT (ASCP) Waterbury Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From Charles.Embrey <@t> carle.com Tue Nov 7 11:56:07 2006 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Tue Nov 7 11:56:14 2006 Subject: [Histonet] cut resistant gloves Message-ID: <44780C571F28624DBB446DE55C4D733A1FE46D@EXCHANGEBE1.carle.com> Cuts in the grossing room are a fairly common occurrence especially with less experienced personnel and sometime with veterans who become overconfident. I nick myself at least once every other year or so when I get in a hurry. Infection risk is low as long as you're cutting fixed tissues. I have, at times, worn cut resistant gloves when doing autopsy but I couldn't gross in them. A lot of my grossing involving large specimens requires for me to feel the lesions or tissue structures and the tactile quality of cut resistant gloves is non-existent. The danger of missing a lesion outweighs the risk of me cutting myself. Charles Embrey, Jr. PA(ASCP) Carle Clinic Illinois -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Tuesday, November 07, 2006 11:28 AM To: Histonet (E-mail) Subject: [Histonet] cut resistant gloves Hi Histonetters - We recently had a serious cut in the grossing room. Does anyone require their personnel to wear cut-resistant liners? If so, for what duties? Do the pathologists routinely wear them? Any other thoughts or comments on the topic are welcome. Thanks, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supv. Laboratory, Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3689 CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Nov 7 12:56:06 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Nov 7 12:56:13 2006 Subject: Fwd: Re: [Histonet] cut resistant gloves Message-ID: <20061107185606.12127.qmail@web61218.mail.yahoo.com> Rene J Buesa wrote: Date: Tue, 7 Nov 2006 09:47:04 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] cut resistant gloves To: Terri Braud Our diener and residents when doing autopsies or when grossing large specimens all were cut-resistant liners always. It is not just a case of being careful, it is a dangerous and cut injury prone task. Ren? J. Terri Braud wrote: Hi Histonetters - We recently had a serious cut in the grossing room. Does anyone require their personnel to wear cut-resistant liners? If so, for what duties? Do the pathologists routinely wear them? Any other thoughts or comments on the topic are welcome. Thanks, Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supv. Laboratory, Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3689 CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Sponsored Link Free Uniden 5.8GHz Phone System with Packet8 Internet Phone Service --------------------------------- Access over 1 million songs - Yahoo! Music Unlimited. From RSRICHMOND <@t> aol.com Tue Nov 7 13:09:35 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Tue Nov 7 13:09:53 2006 Subject: [Histonet] Re: cut resistant gloves Message-ID: <327.f964b0b.328233ef@aol.com> Terri L. Braud, HT(ASCP), Anatomic Pathology Supervisor, Holy Redeemer Hospital, in Meadowbrook, Pennsylvania asks: >>We recently had a serious cut in the grossing room. Does anyone require their personnel to wear cut-resistant liners? If so, for what duties? Do the pathologists routinely wear them?<< In my travels I've rarely seen any special precautions taken. One hospital did have the chain-mail metal glove liners for everybody, but only one pathologist ever used them, a very fastidious man who left nearly all of the autopsy dissection to the (uncertified) autopsy technician. I never got around to trying them, though I meant to. I like a good pair of surgical gloves for the autopsy, and nitrile-rubber gloves for handling tissue in formalin, double-gloving my left hand in deference to the very poor quality of present-day nitrile-rubber gloves. Latex gloves should not be used for handling formaldehyde or xylene. Bob Richmond Samurai Pathologist Knoxville, Tennessee From Melissa.Gonzalez <@t> cellgenesys.com Tue Nov 7 13:13:04 2006 From: Melissa.Gonzalez <@t> cellgenesys.com (Melissa Gonzalez) Date: Tue Nov 7 13:13:17 2006 Subject: [Histonet] RE: F4/80 Message-ID: Hi Susan, I am working on the same thing right now. F4/80 from Serotec used to work fantastically, and for some reason the last few batches have been giving me trouble (from this year). The DOL is just not anywhere what is used to be, and this was a solid marker. I have worked around it by changing up antigen retrievals, but it still is finicky for each different study now. If you wish to use it for fluorescence, use either Molecular Probes AF 488 (green like FITC) or AF594 (red like Texas Red). The Goat anti Rat for 30 minutes at 1:200 RT works like a charm. (for either frozen or paraffin sections). Mount in a good antifade, and you are all set. I'm actually switching back and forth between CalTag's F4/80 and Serotec's now, depending on whichever one gives me the best result. Neither is as good as the last "working" lot of Serotec's. I was curious if others are also are experiencing this problem... Thanks and good luck Susan, Melissa Message: 13 Date: Tue, 7 Nov 2006 14:42:31 -0000 From: Subject: [Histonet] F4/80 To: Message-ID: <90B6684A9D6DAF468F7A5DC148754E1D1E1869@ALPW31.f2.enterprise> Content-Type: text/plain; charset="US-ASCII" Hi Histonetters I have been able to stain some mouse liver for Kuppfer cells using Serotec's F4/80 and copious amounts of help from their tech support, however I would like to use some immunofluorescence instead of the DAB. At present I am using a Goat anti Rat IgG( Mouse adsorbed) :HRP secondary. Does anyone know of another secondary I could use with a fluorescent marker. I am using FFPE or cryostat sections. Thanks Susan From DDittus787 <@t> aol.com Tue Nov 7 13:19:55 2006 From: DDittus787 <@t> aol.com (DDittus787@aol.com) Date: Tue Nov 7 13:20:10 2006 Subject: [Histonet] Fwd: Bubble artifact for Histonet Message-ID: <597.8dcfb45.3282365b@aol.com> Linda In the past in my old lab we would see this off and on also. We found watching the heat of water baths helped and changing the deparaffining xylenes seemed to help a lot with this. I am not sure what mechanism causes this but we (tech's and pathologists) thought it had something to do with heat. Dana From sohail_e <@t> yahoo.com Tue Nov 7 13:42:39 2006 From: sohail_e <@t> yahoo.com (sohail ejaz) Date: Tue Nov 7 13:42:46 2006 Subject: [Histonet] Information regarding tracheal whole mount staining Message-ID: <20061107194239.54969.qmail@web39505.mail.mud.yahoo.com> Hello everyone, Is there anyone who is doing whole mount staining of tracheal vasculature?. I would love if some one is willing to share his porotocol with me. Thanks Sohail --------------------------------- Everyone is raving about the all-new Yahoo! Mail. From tmmrosla <@t> healtheast.org Tue Nov 7 14:23:53 2006 From: tmmrosla <@t> healtheast.org (Mrosla, Tina M) Date: Tue Nov 7 14:31:27 2006 Subject: [Histonet] RE: Bubbling artifact Message-ID: <63FDBA3ACC67464B8F667258229958060F4B7F@EXCHCLUS.healtheast.loc> Michelle, We have had something similar happen in our lab in the past. Our pathologists noticed the bubbling on biopsy slides on occasion, on random slides. We turned down the heat on our slide dryers to around 60 C, and we haven't had any problems since. So, were your slides dried together? Over-heating in the dryers can cause bubbling in tissue (I think I've seen that in Frieda Carson's book somewhere). Also, over-fixation can cause nuclear bubbling, but it sounds like that's not the cause in your case. Tina Mrosla (HTL) St. Joseph's Hospital St. Paul MN 55102 The information included in this e-mail message, including any attachments, is intended only for the person or organization to which it is addressed. This e-mail message may contain information that is privileged or confidential. If you receive this e-mail message and are not the intended recipient or responsible for delivering the message to the intended recipient, you may not use, disseminate, distribute or copy the information included in this e-mail and any attachments. If you received this e-mail message by mistake, please reply by e-mail and destroy all copies of this message and any attachments. Thank you. From bjdewe <@t> aol.com Tue Nov 7 15:15:27 2006 From: bjdewe <@t> aol.com (bjdewe@aol.com) Date: Tue Nov 7 15:15:43 2006 Subject: [Histonet] Tissue processor Message-ID: <8C8D0C8FB282932-980-91EC@mblk-d16.sysops.aol.com> Hi All, We're looking for a very good condition tissue processor. Even refurbished is fine. I don't want to pay 80K that Richard Allen quoted me... Cheers, Lorie Live as if you were to die tomorrow. Learn as if you were to live forever. - Gandhi - ******************************************************************* Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s)and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ******************************************************************* ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From Margaret.Perry <@t> sdstate.edu Tue Nov 7 15:22:44 2006 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Tue Nov 7 15:24:02 2006 Subject: [Histonet] processing poyester filters Message-ID: We have a researcher who is growing porcine intestinal epithelial cells on snapwell polyester filters. He wants us to stain with the PAS and alcian blue. Our first attempt did not work. The filters were fixed in 4% paraformeldehyde for 10 minutes and processed in our regular overnight processing schedule. Our first thought is that we processed way too long. What type of processing schedule should we be using and how long should we be fixing these filters? Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 From candy.a.bales <@t> uth.tmc.edu Tue Nov 7 16:07:51 2006 From: candy.a.bales <@t> uth.tmc.edu (Bales, Candy A) Date: Tue Nov 7 16:07:56 2006 Subject: [Histonet] IF antibody for Sjogren's Message-ID: I am looking for an IF antibody specific for Sjogren's disease. Any and all assistance would be greatly appreciated. Thank you Candy Bales, HT University of Texas Dental Branch Houston, TX From algranth <@t> u.arizona.edu Tue Nov 7 17:31:36 2006 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Tue Nov 7 17:31:48 2006 Subject: [Histonet] processing poyester filters In-Reply-To: Message-ID: <4.3.2.7.2.20061107161953.028d7b08@algranth.inbox.email.arizona.edu> One of my past projects involved processing and sectioning cells grown on corning transwell inserts. Could they be similar to the filters that you are talking about? For these inserts, I processed using our old processor - an old Tissue Tek dip n'dunker for one hour in each station including 2 paraffins (our regular overnite processing schedule). They cut and stained great. I didn't fix them myself but the inserts were flooded with formalin - 10% (as recommended by corning) and allowed to fix for one hour at RT. After fixation they replaced the formalin with 70% ETOH and brought them here to the lab for processing. Andi Grantham At 03:22 PM 11/7/2006 -0600, you wrote: >We have a researcher who is growing porcine intestinal epithelial cells >on snapwell polyester filters. He wants us to stain with the PAS and >alcian blue. Our first attempt did not work. The filters were fixed in >4% paraformeldehyde for 10 minutes and processed in our regular >overnight processing schedule. Our first thought is that we processed >way too long. What type of processing schedule should we be using and >how long should we be fixing these filters? > > > >Margaret Perry HT (ASCP) > >IHC Lab Manager Veterinary Science > >Animal Disease Research and Diagnostic Lab > >South Dakota State University > >Box 2175 North Campus Drive > >Brookings SD 57007 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From rod.coombe <@t> imvs.sa.gov.au Tue Nov 7 18:29:23 2006 From: rod.coombe <@t> imvs.sa.gov.au (Rod Coombe) Date: Tue Nov 7 18:40:04 2006 Subject: [Histonet] Cut Resistant gloves Message-ID: <001701c702cc$f0929270$d86d140a@ITP36079> It is protocol at our institution for all pathologists, scientific / technical staff to wear Kevlar gloves underneath their disposables when grossing. Kevlar gloves aren't failsafe. We have still had injuries with staff wearing Kevlars, but it is assumed the injury would be much worse without them. Rod Coombe Manager Tissue Pathology IMVS Frome Road, Adelaide Phone (08) 8222 3201 / 0401120808 Fax (08) 8222 3204 From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Nov 8 02:49:52 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Nov 8 02:49:01 2006 Subject: [Histonet] cut resistant gloves Message-ID: <86ADE4EB583CE64799A9924684A0FBBFAFC900@wahtntex2.waht.swest.nhs.uk> It's all about reducing risk I expect. I take your point that it is not worth missing lesions because you are wearing 'armoured gloves' but it is not correct that there is no infection risk. With the larger specimens if cut 'unfixed' then there is a risk albeit slight. I guess the interim position is to use these gloves when cutting unfixed material such as lung etc., or when you are trying to cut 'hard' tissue and there's a risk of the knife slipping. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Habits of thinking need not be forever. One of the most significant findings in psychology in the last twenty years is that individuals can choose the way they think. --Martin Seligman This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From c.weaver <@t> vla.defra.gsi.gov.uk Wed Nov 8 04:02:43 2006 From: c.weaver <@t> vla.defra.gsi.gov.uk (Weaver, Colin) Date: Wed Nov 8 04:16:13 2006 Subject: [Histonet] Microwave processing Message-ID: <1172D362B084D311986F0008C79F42C478D2FC@vla22.cvlnt.vla.gov.uk> Hi - I wonder if anyone can tell me what the "special ingredient" long chain hydrocarbon that Surgipath use in their JFC microwave processing solution? Anyone that uses the Surgipath RHS 1 system do you use JFC solution or home prepared Ethanol/Isoprpanol mix? If you use home prepared instead of JFC does it process as satisfactorily as JFC? Colin Weaver Veterinary Laboratories Agency (VLA) This email and any attachments is intended for the named recipient only. If you have received it in error you have no authority to use, disclose, store or copy any of its contents and you should destroy it and inform the sender. Whilst this email and associated attachments will have been checked for known viruses whilst within VLA systems we can accept no responsibility once it has left our systems. Communications on VLA's computer systems may be monitored and/or recorded to secure the effective operation of the system and for other lawful purposes. From mlevin <@t> forsyth.org Wed Nov 8 06:19:23 2006 From: mlevin <@t> forsyth.org (Levin, Michael) Date: Wed Nov 8 06:19:35 2006 Subject: [Histonet] Need recommendation for embedding medium Message-ID: Hello all - I am a developmental biologisy and need a recommendation, to help me choose the right embedding medium. First and simplest, does anyone have a replacement for JB4 that is less toxic? I need something that is transparent (so that I can orient my embryos during embedding), and that I will cut with a vibratome later to make 20 micron sections. I currently use JB4 to section embryos after wholemount immunohistochemistry or in situ hybridization but I need something that is less toxic. Second, I'm looking for a medium that meets the following criteria. I need to be able to embed soft tissues (frog and chick embryos) for sectioning via vibratome (10 microns to 60 microns thick). Unlike with the JB4, I want to do immunohistochemistry on these sections after cutting (but do not need electron microscopy). The embedding medium also needs to be 1) non-toxic (so that a fume hood is not needed during embedding) 2) solidifies into a block that can be cut with vibratome, but not so hard that I can't trim it later with a razor blade 3) is transparent, so that I can orient embryos while embedding Can anyone suggest any kind of embedding mix that matches this description? Please email me at mlevin@forsyth.org. Thank you in advance! Mike From d.gregg <@t> juno.com Wed Nov 8 06:52:15 2006 From: d.gregg <@t> juno.com (Douglas A Gregg) Date: Wed Nov 8 06:53:59 2006 Subject: [Histonet] Fellowship in histotechnology-great experience Message-ID: <20061108.075216.3724.3.d.gregg@juno.com> RESEARCH FELLOWSHIP OPPORTUNITY - HISTOPATHOLOGY ON FOREIGN ANIMAL DISEASES Do you want a research fellowship on an Island with a free scenic ferryboat ride twice a day and work in a BL-3 lab researching exotic diseases of animals? Would you like to live in a resort community surrounded by the Bay or Long Island Sound with great boating, swimming, and fishing? Do you enjoy living in the country in a New England community with easy access to New York City or Boston. We have an immediate opening for a 1-2 year fellowship at the Agricultural Research Service, USDA Foreign Animal Disease Research Unit located at the Plum Island Animal Disease Center on the East end of Long Island, NY . The Plum Island Animal Disease Center Research Participation Program (administered by the Oak Ridge Institute for Science and Education - ORISE) aims to provide educational and research opportunities in pathogenesis of foreign animal diseases such as foot-and-mouth disease and classical swine fever. This fellowship is open to people with at least a bachelors degree and experience in histotechnology. It will provide great opportunities to get a wide variety of experience, including histotech work, cryosectioning, immunohistochemistry, in-situ hybridization, confocal microscopy, and animal necropsy assistance for sample collection in a research atmosphere. You would work in a small lab (one other histotech) with two veterinary pathologists and other microbiologists , molecular biologists and research fellows. The research includes viral pathogenesis and immunopathology of foreign animal diseases. Stipends (ranging from $35,000 to $45,000 a year) will be determined according to education ands experience in the field. In order to reach the Plum Island facility, you will travel daily from Orient Point, NY, or Old Saybrook, CT, aboard a government-provided marine vessel. For more information, you can contact me or read about it at the fellowship website and get an application. www.orau.gov/piadc Douglas Gregg DVM, PhD Veterinary pathologist ARS, USDA, Plum Island Animal Disease Center Greenport, NY 11944 douglas.gregg@ars.usda.gov or phone 631-323-3379 From rjbuesa <@t> yahoo.com Wed Nov 8 07:20:22 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Nov 8 07:20:31 2006 Subject: [Histonet] Microwave processing In-Reply-To: <1172D362B084D311986F0008C79F42C478D2FC@vla22.cvlnt.vla.gov.uk> Message-ID: <20061108132022.79332.qmail@web61223.mail.yahoo.com> It is essentially paraffin oil = mineral oil. If you are interested I can send you a "pdf" with the formula I used to substitute xylene with. Ren? J. "Weaver, Colin" wrote: Hi - I wonder if anyone can tell me what the "special ingredient" long chain hydrocarbon that Surgipath use in their JFC microwave processing solution? Anyone that uses the Surgipath RHS 1 system do you use JFC solution or home prepared Ethanol/Isoprpanol mix? If you use home prepared instead of JFC does it process as satisfactorily as JFC? Colin Weaver Veterinary Laboratories Agency (VLA) This email and any attachments is intended for the named recipient only. If you have received it in error you have no authority to use, disclose, store or copy any of its contents and you should destroy it and inform the sender. Whilst this email and associated attachments will have been checked for known viruses whilst within VLA systems we can accept no responsibility once it has left our systems. Communications on VLA's computer systems may be monitored and/or recorded to secure the effective operation of the system and for other lawful purposes. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Everyone is raving about the all-new Yahoo! Mail. From Barry.R.Rittman <@t> uth.tmc.edu Wed Nov 8 09:49:40 2006 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Wed Nov 8 09:49:51 2006 Subject: [Histonet] TSH Symposium calll for abstracts Message-ID: TEXAS SOCIETY FOR HISTOTECHNOLOGY CALL FOR ABSTRACTS 2007 SYMPOSIUM/CONVENTION APRIL 19-22, 2007 The 2007 TSH Symposium/Convention is scheduled for April 19-22, 2007 at the Renaissance Houston Hotel Greenway Plaza, 6 Greenway Plaza East, Houston, Texas 77046. The convention committee is in the process of developing the program that must be completed by December 2006. If you or your institution have either a workshop or symposium presentation you would like to share with other professionals in our field, please submit an abstract for review. The abstract must include: 1. Title of proposed presentation 2. 150 word abstract of presentation (mandatory for selection) 3. Length of workshop ( 3 or more hours) 4. Length of symposium lecture (1/2 to 2 hours) 5. Equipment List 6. Name, address, and telephone number of presenter(s) 7. Curriculum vita, or backgrounds experience for each presenter, include degrees, certification and/or specialty. SEMINAR AND LECTURE PRESENTATION may include medical, industrial, veterinary, research, plant, marine or other histology techniques or applications and management/personal development. WORKSHOPS may be Basic, Intermediate or advanced on the same topics as listed under lectures. More thane one workshop proposal may be submitted. Limited workshops should have a minimum of 25 registrants. DEADLINE FOR SUBMISSION WILL BE December 1, 2006 PRESENTERS WILL BE SELECTED AND NOTIFIED BY MAIL Handouts are required by February 15, 2007 for symposium lectures and workshops. Materials will be submitted for contact hour approval. Workshop handouts should be limited to 50 pages. All abstracts are acknowledged upon receipt. Contact hour forms will be sent following receipt of the abstract. Notification of acceptance will be mailed by January 2007. Mail completed forms to: Sandra Christiansen Judy Webb Program Chair Vice President,TSH 844 Davis Road OR 3819 Birkdale Drive League City, Texas 77573 Fort Worth, Texas 76116 sandra.christiansen@christushealth.org jwebb01@jpshealth.org TEXAS SOCIETY FOR HISTOTECHNOLOGY SYMPOSIUM/WORKSHOP PROPOSAL NAME: ___________________________________DATE: ________________________ ADDRESS: ______________________________________________________________ ________________________________________________________________________ _ ________________________________________________________________________ _ TELEPHONE: ( ) __________________________FAX: ( )_______________________ TITLE OF WORKSHOP OR SYMPOSIUM: ____________________________________ ________________________________________________________________________ _ ________________________________________________________________________ _ CHECK ONE: * Workshop Half Day * Symposium * Workshop Full Day * Wet Workshop ALL WORKSHOPS MUST BE CONTACT HOUR APPROVED CONTACT HOUR APPROVED: _____YES #______________ _____NO (TSH WILL SUBMIT APPLICATION) Please send abstract, description or outline of the material you wish to present. DEADLINE DATE: December 1, 2007 I have received and agree with the TSH speaker reimbursement policy (Initial)__________ REIMBURSEMENT POLICY FOR SPEAKERS WORKSHOPS Out of town Speakers: Coach airfare (roundtrip) One night lodging for each half-day workshop presented Lunch or voucher for the day of workshop for up to two speakers In town Speakers: Fifty dollar Honorarium Lunch for the day for workshop for up to two speakers Vendor Speaker: Twenty five dollar Honorarium Lunch for the day of workshop for up to two speakers SYMPOSIUMS Out of town Speakers: Seventy-five dollar Honorarium for one speaker only Lunch for the day of symposium for up to two speakers In Town Speakers: Twenty-five dollar Honorarium Lunch for the day of Symposium for up to two speakers From pruegg <@t> ihctech.net Wed Nov 8 10:06:38 2006 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Nov 8 10:06:53 2006 Subject: [Histonet] Information regarding tracheal whole mount staining In-Reply-To: <20061107194239.54969.qmail@web39505.mail.mud.yahoo.com> Message-ID: <003c01c7034f$e0b53460$6501a8c0@Patsy> Sohail, Do you mean dye staining or IHC? I am doing some whole mount IHC on vessels. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sohail ejaz Sent: Tuesday, November 07, 2006 12:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Information regarding tracheal whole mount staining Hello everyone, Is there anyone who is doing whole mount staining of tracheal vasculature?. I would love if some one is willing to share his porotocol with me. Thanks Sohail --------------------------------- Everyone is raving about the all-new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Wed Nov 8 10:27:36 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Nov 8 10:28:21 2006 Subject: [Histonet] F4/80 In-Reply-To: <90B6684A9D6DAF468F7A5DC148754E1D1E1869@ALPW31.f2.enterpris e> References: <90B6684A9D6DAF468F7A5DC148754E1D1E1869@ALPW31.f2.enterprise> Message-ID: <6.0.0.22.1.20061108091145.01b82df8@gemini.msu.montana.edu> Susan, You may need to retiter the primary for immunofluorescence (IFA) staining, generally we find an increase in primary antibody concentration. You will have less formalin induced autofluorescence if you use cryostat sections which also eliminates retrieval. On a fresh tissue cryostat section, fixation with either 75% acetone/25% absolute ethanol for 5 min at RT on overnight dried frozen sections then go directly to pure buffer 3 changes. OR 4C acetone fixation for 10 minutes but air dry after pure acetone fixation. Both will work nicely. If you do FFPE tissues, retrieval and have problems with aldehyde induced autofluorescence, just us a red fluorophore which allows the autofluorescence to become a "counterfluorescence" . You can use Goat antiRat conjugated to FITC, RRX (rhodamine extra) or even a donkey anti Rat conjugated to a fluorophore of choice from Jackson. We prefer F(ab')2 frag of IgG antibodies, adsorbed to mouse to prevent binding to fc receptors on mouse tissues to give cleaner staining. Another source of a secondary is Molecular Probes, and find one adsorbed to mouse conjugated to Alexa 488 (FITC substitute) or Alexa 594 (Texas Red substitute). The reason for 594 is if you ever do double IFA with FITC, you have better separation these two fluorophores. The Alexa dyes are very bright and do not photobleach excessively. Be sure to use an antifade mounting media, Prolong Gold antifade reagent, ready to use hard set from Molecular Probes is excellent and if you want DNA stained, you can buy this with DAPI already in the mounting media. Good luck At 07:42 AM 11/7/2006, you wrote: >Hi Histonetters > >I have been able to stain some mouse liver for Kuppfer cells using >Serotec's F4/80 and copious amounts of help from their tech support, >however I would like to use some immunofluorescence instead of the DAB. >At present I am using a Goat anti Rat IgG( Mouse adsorbed) :HRP >secondary. Does anyone know of another secondary I could use with a >fluorescent marker. I am using FFPE or cryostat sections. > >Thanks > >Susan >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From Margaret.Perry <@t> sdstate.edu Wed Nov 8 10:37:47 2006 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Wed Nov 8 10:37:58 2006 Subject: [Histonet] processing polyester filters Message-ID: I am resending this message because we did not receive Vol 36 Issue 6 of the digest. We have a researcher who is growing porcine intestinal epithelial cells on snapwell polyester filters. He wants us to stain with the PAS and alcian blue. Our first attempt did not work. The filters were fixed in 4% paraformeldehyde for 10 minutes and processed in our regular overnight processing schedule. Our first thought is that we processed way too long. What type of processing schedule should we be using and how long should we be fixing these filters? Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 From SDrew <@t> uwhealth.org Wed Nov 8 10:56:18 2006 From: SDrew <@t> uwhealth.org (Drew Sally A.) Date: Wed Nov 8 10:57:00 2006 Subject: [Histonet] RE: [IHCRG] Tau, Amyloid and Alpha Synuclein for alzheimers In-Reply-To: <4F9A1711C1095543B652EC7EEEC157D00CE06A28@BLUEMAX.VENTANA.VENTANAMED.COM> Message-ID: Debra, we purchase all three of those antibodies Zymed and run them on our Ventana instruments: They work nicely, and If you would like further protocol information, please feel free to contact me. Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 (608)265-6596 -----Original Message----- From: ihcrg@googlegroups.com [mailto:ihcrg@googlegroups.com] On Behalf Of Debra Siena Sent: Monday, November 06, 2006 6:44 PM To: ihcrg@googlegroups.com Subject: [IHCRG] Tau, Amyloid and Alpha Synuclein for alzheimers Hi All, I would like to know if any of you have suggestions as to protocols for these antibodies and also companies that sell Tau, Amyloid and Alpha Synuclein for Alzheimer's disease. Thanks a lot, for all your help. Debbie Siena, HT(ASCP)QIHC Tissue Techniques Pathology Labs 972-241-6277 tissuetech@juno.com --~--~---------~--~----~------------~-------~--~----~ You received this message because you are subscribed to the Google Groups "ihcrg" group. To post to this group, send email to ihcrg@googlegroups.com To unsubscribe from this group, send email to ihcrg-unsubscribe@googlegroups.com For more options, visit this group at http://groups.google.com/group/ihcrg?hl=en -~----------~----~----~----~------~----~------~--~--- From liz <@t> premierlab.com Wed Nov 8 11:12:58 2006 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Nov 8 11:04:38 2006 Subject: [Histonet] processing polyester filters In-Reply-To: Message-ID: <000501c70359$239cf4d0$0300a8c0@domain.Premier> Margaret We have stained mattek cell cultures, we fixed our for 24 hours and then processed routinely. We wrote a Sakura Tissue tek article in Histologic you should be able to see it on line in the archive issues. Our material and methods are included. We have also stained porcine epithial cells that were seeded into plastic tissue scaffolds and we processed those routinely after fixation and those also worked just fine. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Perry, Margaret Sent: Wednesday, November 08, 2006 9:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] processing polyester filters I am resending this message because we did not receive Vol 36 Issue 6 of the digest. We have a researcher who is growing porcine intestinal epithelial cells on snapwell polyester filters. He wants us to stain with the PAS and alcian blue. Our first attempt did not work. The filters were fixed in 4% paraformeldehyde for 10 minutes and processed in our regular overnight processing schedule. Our first thought is that we processed way too long. What type of processing schedule should we be using and how long should we be fixing these filters? Margaret Perry HT (ASCP) IHC Lab Manager Veterinary Science Animal Disease Research and Diagnostic Lab South Dakota State University Box 2175 North Campus Drive Brookings SD 57007 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1.1859 (20061108) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From RSRICHMOND <@t> aol.com Wed Nov 8 12:10:53 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Wed Nov 8 12:11:07 2006 Subject: [Histonet] Re: Cut resistant gloves Message-ID: <3f6.3536b649.328377ad@aol.com> Rod Coombe in Adelaide, Down Under notes: >>It is protocol at our institution for all pathologists, scientific, and technical staff to wear Kevlar gloves underneath their disposables when grossing.< < Where do you get Kevlar gloves? What do they cost? How do you maintain them? How long do they last if you don't puncture them? Bob Richmond Samurai Pathologist Knoxville, Tennessee From RSRICHMOND <@t> aol.com Wed Nov 8 12:14:25 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Wed Nov 8 12:14:42 2006 Subject: [Histonet] Re: nuclear bubble artifact Message-ID: <490.d45412a.32837881@aol.com> I know this subject has come up before, and I may have missed it on Histonet, but... How do you trouble shoot nuclear bubble artifact? Nuclear bubble artifact really degrades pathologic diagnosis. I've never been clear how to advise a histotechnologist on how to look for the cause and fix it. Can some of the senior histotechnologists on HIstonet address this issue for all of us? Bob Richmond Samurai Pathologist Knoxville TN From Vickroy.Jim <@t> mhsil.com Wed Nov 8 12:35:21 2006 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Wed Nov 8 12:36:04 2006 Subject: [Histonet] processing possible TB infected tissue Message-ID: We are reviewing our procedures and have a procedure that we really haven't been following. This is a procedure that calls for processing possible TB tissue by hand. This was based on an old report that possible TB bacteria could survive formalin fixation. Right now we are processing all of our tissue by automated tissue processors, and we really only handle CJD tissue differently than the others. Obviously we don't have many cases suspected of TB but we do occasionally process granulomas that are suspicious for AFB positive organisms. The final diagnosis of these organisms is usually not known until long after the processing. I am interested hearing how other people handle this tissue. Thanks. Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From gcallis <@t> montana.edu Wed Nov 8 13:04:51 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Nov 8 13:05:11 2006 Subject: [Histonet] Re: nuclear bubble artifact In-Reply-To: <490.d45412a.32837881@aol.com> References: <490.d45412a.32837881@aol.com> Message-ID: <6.0.0.22.1.20061108115949.01b4cd70@gemini.msu.montana.edu> We created nuclear bubble artifact by flattening a just picked up section (from waterbath) on a really hot surface or hot plate. One person just addressed this today by saying too hot a waterbath contributes to this also. We quit flattening the section on a hot plate, and simply drained the slide, then went to a lower heat incubator - 60C is probably adequate for a short time or until paraffin is melted. At 11:14 AM 11/8/2006, you wrote: >I know this subject has come up before, and I may have missed it on Histonet, >but... > >How do you trouble shoot nuclear bubble artifact? > >Nuclear bubble artifact really degrades pathologic diagnosis. I've never been >clear how to advise a histotechnologist on how to look for the cause and fix >it. Can some of the senior histotechnologists on HIstonet address this issue >for all of us? > >Bob Richmond >Samurai Pathologist >Knoxville TN >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From gcallis <@t> montana.edu Wed Nov 8 13:29:19 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Nov 8 13:29:47 2006 Subject: [Histonet] vasculature of trachea Message-ID: <6.0.0.22.1.20061108122449.01b22520@gemini.msu.montana.edu> Vector laboratories just sent a flyer on using a tomato lectin conjugated to rhodamine for staining blood vessels in tissues. Go to www.vectorlabs.com and read their latest VECTAGRAM. For further and specific methods, go to American Journal of Pathology, 166(3), 2005 for a publication on using this method. It can be done by intravascular perfusion or on tissue sections. The publication used a FITC labeled tomato lectin and was quite elegant. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From mcauliff <@t> umdnj.edu Wed Nov 8 13:52:50 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Wed Nov 8 13:52:25 2006 Subject: [Histonet] vasculature of trachea In-Reply-To: <6.0.0.22.1.20061108122449.01b22520@gemini.msu.montana.edu> References: <6.0.0.22.1.20061108122449.01b22520@gemini.msu.montana.edu> Message-ID: <45523592.4010404@umdnj.edu> I have forgotten who the original poster was but I just had a flashback ..... Years ago (and I do mean early 1970's or so) there was a paper in Stain Technology (now Biotechnic and Histochemistry) about demonstrating vasculature in kidney (or ?) by doing a benzidine (or diaminobenzidine) reaction for blood in vessels. If one was to clamp the vein draining the organ first the vasculature would fill, then clamp the artery. Remove and fix in something that won't lyse RBC, cut sections as thick or thin as you like, and do the reaction. Geoff Gayle Callis wrote: > Vector laboratories just sent a flyer on using a tomato lectin > conjugated to rhodamine for staining blood vessels in tissues. Go to > www.vectorlabs.com and read their latest VECTAGRAM. > > For further and specific methods, go to American Journal of Pathology, > 166(3), 2005 for a publication on using this method. It can be done > by intravascular perfusion or on tissue sections. The publication > used a FITC labeled tomato lectin and was quite elegant. > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 > 406 994-4303 (FAX) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From cytologer <@t> msn.com Thu Nov 9 01:19:43 2006 From: cytologer <@t> msn.com (ChoiUl Soo) Date: Thu Nov 9 01:19:50 2006 Subject: [Histonet] about methyl green pyronin stain for plasmacytoma... Message-ID: Hi histonetters, I amwondering if anyone would tell me the limitations of methyl green pyronin for plasmacytoma. Though I am looking foolish, I think RNAs would increase in the cytoplasms of most actively proliferating tumor cells, so I am curious is it possible to use this stain to differentiate plasmacytoma cells from other tumor cells based on the pyroninophilia of the cytoplasm. I remember reading somewhere this stain is non specific, but I could not find it....please help me, clarify me....on this subject Thanks in advance, Ul Soo Choi DVM, PhD VMTH 208, CVM, Seoul National University, Shilim9 dong, Gwanakgu, Seoul, Korea 151-742 Tel. 82-02-880-8688 Mobile. 82-016-9228-8634 Fax. 82-02-880-8662 _________________________________________________________________ ??? ???? ?????? ?????. ??? Global passport, Windows Live Spaces! http://spaces.live.com/signup.aspx From yjones2 <@t> csmlab.com Thu Nov 9 02:18:04 2006 From: yjones2 <@t> csmlab.com (Yvonne Jones) Date: Thu Nov 9 02:19:22 2006 Subject: [Histonet] We are having a lot of difficulty finding an inexpensive yet reliable source for helicobactor pylori Message-ID: <45529DEC0200004F000002F1@GWGATE1.ahm.com> We are having a lot of difficulty finding an inexpensive yet reliable source for helicobactor pylori control tissue. We are currently purchasing them, but often they don't work consistently, and we've gotten mixed results using slides even from the same box. In short, we need a new source. This is my question: We have had success culturing all of our other bacteria; then after we grow a sufficient amount, we inject a liquid suspension into the lumen of appendix, fix and process the appendix and use the resulting tissue sections as positive controls (works for pcp, gram, spirochete, etc.). However, we can't seem to get h.pylori to grow the same way. Is anyone else out there using this technique to make controls, and if so have you had the same problems growing helico? And would it be acceptable to use camphy bacter instead (which I've heard is more hearty and easy to culture)?----------------------------------------- From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Nov 9 02:55:06 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Nov 9 02:54:14 2006 Subject: [Histonet] Re: nuclear bubble artifact Message-ID: <86ADE4EB583CE64799A9924684A0FBBFAFC910@wahtntex2.waht.swest.nhs.uk> As I have already said, poor fixation followed by elevated heat in floating out or drying. To fix, fix better, or post fix and don't use elevated temperatures. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Habits of thinking need not be forever. One of the most significant findings in psychology in the last twenty years is that individuals can choose the way they think. --Martin Seligman This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Nov 9 02:55:41 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Nov 9 02:54:45 2006 Subject: [Histonet] Re: Cut resistant gloves Message-ID: <86ADE4EB583CE64799A9924684A0FBBFAFC911@wahtntex2.waht.swest.nhs.uk> Motorbike shops; I've got some. Really kewl looking. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Habits of thinking need not be forever. One of the most significant findings in psychology in the last twenty years is that individuals can choose the way they think. --Martin Seligman This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Nov 9 04:11:56 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Nov 9 04:11:03 2006 Subject: [Histonet] about methyl green pyronin stain for plasmacytoma... Message-ID: <86ADE4EB583CE64799A9924684A0FBBFAFC918@wahtntex2.waht.swest.nhs.uk> I amwondering if anyone would tell me the limitations of methyl green pyronin for plasmacytoma. Though I am looking foolish, I think RNAs would increase in the cytoplasms of most actively proliferating tumor cells, so I am curious is it possible to use this stain to differentiate plasmacytoma cells from other tumor cells based on the pyroninophilia of the cytoplasm. I remember reading somewhere this stain is non specific, but I could not find it....please help me, clarify me....on this subject Methyl Green Pyronin, if used correctly will differentiate between DNA (green) and RNA (red). It is best after neutral and acid alcoholic fixatives (Carnoy) as fixation affects staining. I regard to your question I guess (and I'm not a Histopathologist) that you would have to have plasmacytoma as a differential in your diagnosis and the stain used as an aid. I don't think you can use the stain 'litmus' for this type of tumour as that would make too easy. I guess there are many conditions that result in an increase in the amount of RNA in a nuclease other than just malignancy. It is differentiates between DNA and RNA but is not specific (bacteria stain red too) and is therefore an aid. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Habits of thinking need not be forever. One of the most significant findings in psychology in the last twenty years is that individuals can choose the way they think. --Martin Seligman This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From lpwenk <@t> sbcglobal.net Thu Nov 9 04:37:04 2006 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Nov 9 04:37:23 2006 Subject: [Histonet] We are having a lot of difficulty finding an inexpensiveyet reliable source for helicobactor pylori In-Reply-To: <45529DEC0200004F000002F1@GWGATE1.ahm.com> Message-ID: <002801c703eb$0123f4c0$baec2d4b@HPPav2> It's very difficult to culture Helicobacter. It takes a special media (with iron ions and mucin and horse serum) and at near anaerobic conditions. When you find positive biopsies, cut additional 10-20 slides, 1 section each. There will still be enough tissue left in the block, and you will have your control slides. Also, many (unstained) micro-organims control slides, over time, will show decrease to no staining. So cut enough for 3-6 months. Sometimes, with some commercial companies, they cut their slides and store them until someone orders them, which could be a year to two later. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Yvonne Jones Sent: Thursday, November 09, 2006 3:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] We are having a lot of difficulty finding an inexpensiveyet reliable source for helicobactor pylori We are having a lot of difficulty finding an inexpensive yet reliable source for helicobactor pylori control tissue. We are currently purchasing them, but often they don't work consistently, and we've gotten mixed results using slides even from the same box. In short, we need a new source. This is my question: We have had success culturing all of our other bacteria; then after we grow a sufficient amount, we inject a liquid suspension into the lumen of appendix, fix and process the appendix and use the resulting tissue sections as positive controls (works for pcp, gram, spirochete, etc.). However, we can't seem to get h.pylori to grow the same way. Is anyone else out there using this technique to make controls, and if so have you had the same problems growing helico? And would it be acceptable to use camphy bacter instead (which I've heard is more hearty and easy to culture)?----------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Thu Nov 9 04:39:29 2006 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Nov 9 04:39:46 2006 Subject: [Histonet] Autopsy training videos Message-ID: <002901c703eb$5670cf20$baec2d4b@HPPav2> Does anyone know where there are training videos/CD/DVD for how to do an autopsy. Looking for something to supplement teaching first year residents and new dieners (people who assist with the autopsies). Looking on the internet gets me into sites I don't want to get into. There are some sick people out there. Thanks. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 From Terry.Marshall <@t> rothgen.nhs.uk Thu Nov 9 05:41:01 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Thu Nov 9 05:41:02 2006 Subject: [Histonet] Re: Cut resistant gloves Message-ID: <63984BC3A63FF542AF6EF0A237F38F4D7E4C8E@LIL.xRothGen.nhs.uk> Would you cut up in them? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk] Sent: 09 November 2006 08:56 To: RSRICHMOND@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Cut resistant gloves Motorbike shops; I've got some. Really kewl looking. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Habits of thinking need not be forever. One of the most significant findings in psychology in the last twenty years is that individuals can choose the way they think. --Martin Seligman This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Nov 9 06:06:40 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Nov 9 06:05:49 2006 Subject: [Histonet] Re: Cut resistant gloves Message-ID: <86ADE4EB583CE64799A9924684A0FBBFAFC91B@wahtntex2.waht.swest.nhs.uk> I've cut many a Car Driver up in them!! Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Habits of thinking need not be forever. One of the most significant findings in psychology in the last twenty years is that individuals can choose the way they think. --Martin Seligman This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Terry.Marshall <@t> rothgen.nhs.uk Thu Nov 9 06:16:20 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Thu Nov 9 06:16:03 2006 Subject: [Histonet] Re: Cut resistant gloves Message-ID: <63984BC3A63FF542AF6EF0A237F38F4D7E4C8F@LIL.xRothGen.nhs.uk> I remember your driving, but would you do the sort of cut up of which we are talking in them? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk] Sent: 09 November 2006 12:07 To: Marshall Terry Dr, Consultant Histopathologist; RSRICHMOND@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Cut resistant gloves I've cut many a Car Driver up in them!! Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Habits of thinking need not be forever. One of the most significant findings in psychology in the last twenty years is that individuals can choose the way they think. --Martin Seligman This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From sbreeden <@t> nmda.nmsu.edu Thu Nov 9 09:00:10 2006 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Nov 9 09:00:18 2006 Subject: [Histonet] Special Stain "Storage" Message-ID: <4D14F0FC9316DD41972D5F03C070908B0DB315@nmdamailsvr.nmda.ad.nmsu.edu> Someone out there has a clever idea and I'd like to tap that ingenuity. I have an overhead cabinet where I keep my special stains that don't require refrigeration. I'd like to keep each stain set together on the shelf - like in some kind of box. What do you use? One thing that comes to mind is sturdy plastic boxes that Lenscrafters, etc., use - roughly 9x6x3" (I've offered to take any extras but they are very fond of them and won't give 'em up!). Anyway, what do you use that keeps all the bottles together on the shelf? I'd love to have your input and I'll put you in my memoirs. Friday Hour of Fun is comin' up... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 From JWEEMS <@t> sjha.org Thu Nov 9 09:03:38 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Thu Nov 9 09:03:59 2006 Subject: [Histonet] Special Stain "Storage" Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEF124@sjhaexc02.sjha.org> I used to use plastic baskets that you can find at discount stores - usually very cheap. Looking forward to any fun that might come this way! j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Breeden, Sara Sent: Thursday, November 09, 2006 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special Stain "Storage" Someone out there has a clever idea and I'd like to tap that ingenuity. I have an overhead cabinet where I keep my special stains that don't require refrigeration. I'd like to keep each stain set together on the shelf - like in some kind of box. What do you use? One thing that comes to mind is sturdy plastic boxes that Lenscrafters, etc., use - roughly 9x6x3" (I've offered to take any extras but they are very fond of them and won't give 'em up!). Anyway, what do you use that keeps all the bottles together on the shelf? I'd love to have your input and I'll put you in my memoirs. Friday Hour of Fun is comin' up... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From lblazek <@t> digestivespecialists.com Thu Nov 9 09:15:41 2006 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Nov 9 09:16:01 2006 Subject: [Histonet] Special Stain "Storage" Message-ID: <6CBA6DC98A079D408C87250591D9DFB802684BB7@bruexchange.digestivespecialists.com> Hi Sally, I use the plastic container and basket that you can find at Walmart and such. Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, November 09, 2006 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special Stain "Storage" Someone out there has a clever idea and I'd like to tap that ingenuity. I have an overhead cabinet where I keep my special stains that don't require refrigeration. I'd like to keep each stain set together on the shelf - like in some kind of box. What do you use? One thing that comes to mind is sturdy plastic boxes that Lenscrafters, etc., use - roughly 9x6x3" (I've offered to take any extras but they are very fond of them and won't give 'em up!). Anyway, what do you use that keeps all the bottles together on the shelf? I'd love to have your input and I'll put you in my memoirs. Friday Hour of Fun is comin' up... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From funderwood <@t> mcohio.org Thu Nov 9 09:39:46 2006 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Thu Nov 9 09:40:27 2006 Subject: [Histonet] Special Stain "Storage" Message-ID: Hi Sally, I use rectangular organizer bins. Lowes and Home Depot carry them. Go to Market Lab's web site and search organizer bin to get a look see. They are pretty sweet for a variety of lab uses. Fred >>> "Breeden, Sara" 11/9/2006 10:00 AM >>> Someone out there has a clever idea and I'd like to tap that ingenuity. I have an overhead cabinet where I keep my special stains that don't require refrigeration. I'd like to keep each stain set together on the shelf - like in some kind of box. What do you use? One thing that comes to mind is sturdy plastic boxes that Lenscrafters, etc., use - roughly 9x6x3" (I've offered to take any extras but they are very fond of them and won't give 'em up!). Anyway, what do you use that keeps all the bottles together on the shelf? I'd love to have your input and I'll put you in my memoirs. Friday Hour of Fun is comin' up... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Thu Nov 9 09:37:08 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Thu Nov 9 09:41:10 2006 Subject: [Histonet] Re: nuclear bubble artifact Message-ID: <63984BC3A63FF542AF6EF0A237F38F4D7E4C92@LIL.xRothGen.nhs.uk> Firstly, they are not nuclear, but often more conspicuous in nuclei because of contrast. You do what I have spent countless hours doing - changing times reagents and anything else that you can think of. Heat is invariably mentioned as a cause, and it certainly may be a causative factor, but although when Kemlo and I originally tackled this years ago, we eliminated it by cooling everything down, this did not work when I was in Oman, and spent countless hours fiddling. The crux of the matter is to find out what the bubbles are due to. For sure, it's something that is or has been beneath the section. For sure it's not a stray Calorie or two. I say section, but I have seen them in cytology preparations as well as frozen sections, trashing the sometimes suggested cause of residual wax. I have a theory on which I may work, but I'm keeping it close to my chest. How much is a Nobel prize worth? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: RSRICHMOND@aol.com [mailto:RSRICHMOND@aol.com] Sent: 08 November 2006 18:14 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: nuclear bubble artifact I know this subject has come up before, and I may have missed it on Histonet, but... How do you trouble shoot nuclear bubble artifact? Nuclear bubble artifact really degrades pathologic diagnosis. I've never been clear how to advise a histotechnologist on how to look for the cause and fix it. Can some of the senior histotechnologists on HIstonet address this issue for all of us? Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sclark59 <@t> hotmail.com Thu Nov 9 09:43:14 2006 From: sclark59 <@t> hotmail.com (Stephen Clark) Date: Thu Nov 9 09:42:42 2006 Subject: [Histonet] Autofluorescence Message-ID: My name is Stephen Clark and i work in the neuro lab at Eastern Illinois University. We use fluorescent immunohistochemistry to stain the Olfactory Epithelium of mice and have recently had a problem with autofluorescence of our tissues. They are fixed in 4% paraformaldehyde in PBS and i have already tried sodium borohydrate with little success. I have read about photobleaching using UV, neon, and lights of specific wavelengths (488 and 633nm). I am just wondering if anyone utilizes photobleaching via a light source and where it would be possible to purchase them. I am also wondering if the 18um sections might be a little too thick and whether that would have an increased affect on autofluorescence. Any tips would be appreciated. From Lynne.Bell <@t> hitchcock.org Thu Nov 9 09:56:34 2006 From: Lynne.Bell <@t> hitchcock.org (Bell, Lynne) Date: Thu Nov 9 09:56:43 2006 Subject: [Histonet] Special Stain "Storage" In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B0DB315@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: Cheapskate that I am.........I purchased plastic shoe boxes without the lids at the Dollar Tree dollar store. I labeled the front of them with the name of the stain. I'll take a large glass of white Zinfandel for my Friday Hour of Fun, please. From pmarcum <@t> vet.upenn.edu Thu Nov 9 09:58:14 2006 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Thu Nov 9 09:58:24 2006 Subject: [Histonet] Special Stain "Storage" In-Reply-To: <6CBA6DC98A079D408C87250591D9DFB802684BB7@bruexchange.diges tivespecialists.com> References: <6CBA6DC98A079D408C87250591D9DFB802684BB7@bruexchange.digestivespecialists.com> Message-ID: <6.2.5.6.2.20061109105742.01c21948@vet.upenn.edu> That's where we found ours and even had a choice of sizes. Pam Marcum At 10:15 AM 11/9/2006, Blazek, Linda wrote: >Hi Sally, >I use the plastic container and basket that you can find at Walmart and >such. Linda > >Linda Blazek HT (ASCP) >Manager/Supervisor >GI Pathology of Dayton >7415 Brandt Pike >Huber Heights, OH 45424 >Phone: (937) 293-4424 ext 7118 >Email: lblazek@digestivespecialists.com > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, >Sara >Sent: Thursday, November 09, 2006 10:00 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Special Stain "Storage" > >Someone out there has a clever idea and I'd like to tap that ingenuity. >I have an overhead cabinet where I keep my special stains that don't >require refrigeration. I'd like to keep each stain set together on the >shelf - like in some kind of box. What do you use? One thing that comes >to mind is sturdy plastic boxes that Lenscrafters, etc., use - roughly >9x6x3" (I've offered to take any extras but they are very fond of them >and won't give 'em up!). Anyway, what do you use that keeps all the >bottles together on the shelf? I'd love to have your input and I'll put >you in my memoirs. > > > >Friday Hour of Fun is comin' up... > > > >Sally Breeden, HT(ASCP) > >NM Dept. of Agriculture > >Veterinary Diagnostic Services > >PO Box 700 > >Albuquerque, NM 87106 > >505-841-2576 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From Terry.Marshall <@t> rothgen.nhs.uk Thu Nov 9 10:12:20 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Thu Nov 9 10:12:20 2006 Subject: [Histonet] Off topic - Friday love story Message-ID: <63984BC3A63FF542AF6EF0A237F38F4D7E4C93@LIL.xRothGen.nhs.uk> I just had to send this - it is so moving. %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% An elderly man lay dying in his bed. While suffering the agonies of impending death, he suddenly smelled the aroma of his favorite biscuits wafting up the stairs. He gathered his remaining strength, and lifted himself from the bed. Leaning on the wall, he slowly made his way out of the bedroom, and with even greater effort, gripping the railing with both hands, he crawled downstairs. With laboured breath, he leaned against the door-frame, gazing into the kitchen. Were it not for death's agony, he would have thought himself already in heaven, for there, spread out upon waxed paper on the kitchen table were literally hundreds of his favourite biscuits. Was it heaven? Or was it one final act of love from his devoted wife of sixty years, seeing to it that he left this world a happy man? Mustering one great final effort, he threw himself towards the table, landing on his knees in crumpled posture. His aged and withered hand trembled towards a biscuit at the edge of the table, when it was suddenly smacked by his wife with a spatula............. "F#ck off" she said, "They're for the funeral." Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk From JWEEMS <@t> sjha.org Thu Nov 9 10:14:49 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Thu Nov 9 10:15:11 2006 Subject: [Histonet] Off topic - Friday love story Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEF12D@sjhaexc02.sjha.org> the fun has begun!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Thursday, November 09, 2006 11:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Off topic - Friday love story I just had to send this - it is so moving. %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% An elderly man lay dying in his bed. While suffering the agonies of impending death, he suddenly smelled the aroma of his favorite biscuits wafting up the stairs. He gathered his remaining strength, and lifted himself from the bed. Leaning on the wall, he slowly made his way out of the bedroom, and with even greater effort, gripping the railing with both hands, he crawled downstairs. With laboured breath, he leaned against the door-frame, gazing into the kitchen. Were it not for death's agony, he would have thought himself already in heaven, for there, spread out upon waxed paper on the kitchen table were literally hundreds of his favourite biscuits. Was it heaven? Or was it one final act of love from his devoted wife of sixty years, seeing to it that he left this world a happy man? Mustering one great final effort, he threw himself towards the table, landing on his knees in crumpled posture. His aged and withered hand trembled towards a biscuit at the edge of the table, when it was suddenly smacked by his wife with a spatula............. "F#ck off" she said, "They're for the funeral." Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From ree3 <@t> leicester.ac.uk Thu Nov 9 10:22:32 2006 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Thu Nov 9 10:22:50 2006 Subject: [Histonet] Off topic - Friday love story In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEF12D@sjhaexc02.sjha.org> Message-ID: And it is not Friday yet!!!. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: 09 November 2006 16:15 To: Marshall Terry Dr,Consultant Histopathologist; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Off topic - Friday love story the fun has begun!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Thursday, November 09, 2006 11:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Off topic - Friday love story I just had to send this - it is so moving. %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% %%%%%%%% An elderly man lay dying in his bed. While suffering the agonies of impending death, he suddenly smelled the aroma of his favorite biscuits wafting up the stairs. He gathered his remaining strength, and lifted himself from the bed. Leaning on the wall, he slowly made his way out of the bedroom, and with even greater effort, gripping the railing with both hands, he crawled downstairs. With laboured breath, he leaned against the door-frame, gazing into the kitchen. Were it not for death's agony, he would have thought himself already in heaven, for there, spread out upon waxed paper on the kitchen table were literally hundreds of his favourite biscuits. Was it heaven? Or was it one final act of love from his devoted wife of sixty years, seeing to it that he left this world a happy man? Mustering one great final effort, he threw himself towards the table, landing on his knees in crumpled posture. His aged and withered hand trembled towards a biscuit at the edge of the table, when it was suddenly smacked by his wife with a spatula............. "F#ck off" she said, "They're for the funeral." Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Nov 9 10:25:59 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Nov 9 10:25:09 2006 Subject: [Histonet] Off topic - Friday love story Message-ID: <86ADE4EB583CE64799A9924684A0FBBFAFC923@wahtntex2.waht.swest.nhs.uk> The biscuits were for her lover as she had been cheating on him for the last 10 years cos of his impotence? So moving, so moving, I can feel the tears welling. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Habits of thinking need not be forever. One of the most significant findings in psychology in the last twenty years is that individuals can choose the way they think. --Martin Seligman This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From POWELL_SA <@t> Mercer.edu Thu Nov 9 10:29:30 2006 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Thu Nov 9 10:30:16 2006 Subject: [Histonet] Special Stain "Storage" In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B0DB315@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <01M9CUOHJ9848X3HZZ@Macon2.Mercer.edu> Wal-Mart shoe boxes work, also the Dollar Stores, Dollar Tree, etc have very inexpensive basket configurations that can be used. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Thursday, November 09, 2006 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special Stain "Storage" Someone out there has a clever idea and I'd like to tap that ingenuity. I have an overhead cabinet where I keep my special stains that don't require refrigeration. I'd like to keep each stain set together on the shelf - like in some kind of box. What do you use? One thing that comes to mind is sturdy plastic boxes that Lenscrafters, etc., use - roughly 9x6x3" (I've offered to take any extras but they are very fond of them and won't give 'em up!). Anyway, what do you use that keeps all the bottles together on the shelf? I'd love to have your input and I'll put you in my memoirs. Friday Hour of Fun is comin' up... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Arlene.Lim <@t> umassmed.edu Thu Nov 9 10:31:40 2006 From: Arlene.Lim <@t> umassmed.edu (Lim, Arlene) Date: Thu Nov 9 10:32:00 2006 Subject: [Histonet] H&E staining protocol for mouse liver cryosections Message-ID: Hi, I am doing H&E staining on fixed mouse liver cryosections and I can't seem to get a decent section. After staining with H&E, there seems to be air pockets between cells and the cells appear overdried. Someone suggested that I immerse the slides in formalin immediately after sectioning and when I did that, my sections floated away (I am using Superfrost plus slides). Also tried it in 95% ethanol and I still lost my samples. Air drying for about 10 minutes prevented the sections from floating but then my sections get "cracked". It seems that I get small pockets or cracks as soon as the sections get transferred onto the slides and they become bigger during processing. I appreciate any help you can throw my way. Thanks! Arlene University of Massachusetts Worcester, MA From Janet.Bonner <@t> FLHOSP.ORG Thu Nov 9 10:31:07 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Thu Nov 9 10:32:04 2006 Subject: [Histonet] Off topic - Friday love story References: Message-ID: <5F31F38C96781A4FBE3196EBC22D478004A696@fhosxchmb006.ADVENTISTCORP.NET> Close enough! ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Edwards, R.E. Sent: Thu 11/9/2006 11:22 AM To: Weems, Joyce; Marshall Terry Dr,Consultant Histopathologist; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Off topic - Friday love story And it is not Friday yet!!!. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: 09 November 2006 16:15 To: Marshall Terry Dr,Consultant Histopathologist; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Off topic - Friday love story the fun has begun!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Thursday, November 09, 2006 11:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Off topic - Friday love story I just had to send this - it is so moving. %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% %%%%%%%% An elderly man lay dying in his bed. While suffering the agonies of impending death, he suddenly smelled the aroma of his favorite biscuits wafting up the stairs. He gathered his remaining strength, and lifted himself from the bed. Leaning on the wall, he slowly made his way out of the bedroom, and with even greater effort, gripping the railing with both hands, he crawled downstairs. With laboured breath, he leaned against the door-frame, gazing into the kitchen. Were it not for death's agony, he would have thought himself already in heaven, for there, spread out upon waxed paper on the kitchen table were literally hundreds of his favourite biscuits. Was it heaven? Or was it one final act of love from his devoted wife of sixty years, seeing to it that he left this world a happy man? Mustering one great final effort, he threw himself towards the table, landing on his knees in crumpled posture. His aged and withered hand trembled towards a biscuit at the edge of the table, when it was suddenly smacked by his wife with a spatula............. "F#ck off" she said, "They're for the funeral." Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From Janet.Bonner <@t> FLHOSP.ORG Thu Nov 9 10:32:17 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Thu Nov 9 10:34:29 2006 Subject: [Histonet] Off topic - Friday love story References: <86ADE4EB583CE64799A9924684A0FBBFAFC923@wahtntex2.waht.swest.nhs.uk> Message-ID: <5F31F38C96781A4FBE3196EBC22D478004A697@fhosxchmb006.ADVENTISTCORP.NET> OK - Let's everyone Google: Biscuits/Impotence and see whose name comes up with this subject!!!! ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kemlo Rogerson Sent: Thu 11/9/2006 11:25 AM To: Marshall Terry Dr,Consultant Histopathologist; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Off topic - Friday love story The biscuits were for her lover as she had been cheating on him for the last 10 years cos of his impotence? So moving, so moving, I can feel the tears welling. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Habits of thinking need not be forever. One of the most significant findings in psychology in the last twenty years is that individuals can choose the way they think. --Martin Seligman This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From godsgalnow <@t> aol.com Thu Nov 9 10:34:13 2006 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Thu Nov 9 10:34:31 2006 Subject: [Histonet] TABLE OF CONTENTS Message-ID: <8C8D23406BA318F-76C-797B@WEBMAIL-MA17.sysops.aol.com> Does anyone out there have a table of contents to their procedure manual that they are willing to share? I would greatly appreciate it......... Roxanne Soto HT(ASCP)QIHC Lab Manager PhysiciansRightPath Tamap, FL 813-549-1050 ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From tbraud <@t> holyredeemer.com Thu Nov 9 10:41:38 2006 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Thu Nov 9 10:43:24 2006 Subject: [Histonet] Special Stain "Storage" In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B0DB315@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: In the past, I've used the cheap plastic shoe boxes available at almost any dollar store. They're a nice standard size, (throw the lids away). In one lab where I was mgr, the special stains supervisor took the idea a step further, and had a box for each stain offered. It had all the room temp reagents, PLUS an index card with a shortened "quick" procedure, including a list of the frozen or refrigerated reagents needed, PLUS the control slides for that stain. She is now long retired, but she had some awsome ideas! Is it Friday yet? Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supv. Laboratory, Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3689 -----Original Message----- Subject: [Histonet] Special Stain "Storage" Someone out there has a clever idea and I'd like to tap that ingenuity. I have an overhead cabinet where I keep my special stains that don't require refrigeration. I'd like to keep each stain set together on the shelf - like in some kind of box. What do you use? One thing that comes to mind is sturdy plastic boxes that Lenscrafters, etc., use - roughly 9x6x3" (I've offered to take any extras but they are very fond of them and won't give 'em up!). Anyway, what do you use that keeps all the bottles together on the shelf? I'd love to have your input and I'll put you in my memoirs. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. From ree3 <@t> leicester.ac.uk Thu Nov 9 10:46:55 2006 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Thu Nov 9 10:47:08 2006 Subject: [Histonet] Off topic - Friday love story In-Reply-To: <5F31F38C96781A4FBE3196EBC22D478004A697@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: I tried that and got a recipe for gingerbread men?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonner, Janet Sent: 09 November 2006 16:32 To: Kemlo Rogerson; Marshall Terry Dr,Consultant Histopathologist; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Off topic - Friday love story OK - Let's everyone Google: Biscuits/Impotence and see whose name comes up with this subject!!!! ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kemlo Rogerson Sent: Thu 11/9/2006 11:25 AM To: Marshall Terry Dr,Consultant Histopathologist; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Off topic - Friday love story The biscuits were for her lover as she had been cheating on him for the last 10 years cos of his impotence? So moving, so moving, I can feel the tears welling. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Habits of thinking need not be forever. One of the most significant findings in psychology in the last twenty years is that individuals can choose the way they think. --Martin Seligman This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> Mercer.edu Thu Nov 9 10:48:04 2006 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Thu Nov 9 10:48:51 2006 Subject: [Histonet] Special Stain "Storage" In-Reply-To: Message-ID: <01M9CVBILKFU8X3LFR@Macon2.Mercer.edu> No don't throw the lids away, save them to serve the wine on Friday. :) Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Thursday, November 09, 2006 11:42 AM To: Histonet (E-mail) Subject: RE: [Histonet] Special Stain "Storage" In the past, I've used the cheap plastic shoe boxes available at almost any dollar store. They're a nice standard size, (throw the lids away). In one lab where I was mgr, the special stains supervisor took the idea a step further, and had a box for each stain offered. It had all the room temp reagents, PLUS an index card with a shortened "quick" procedure, including a list of the frozen or refrigerated reagents needed, PLUS the control slides for that stain. She is now long retired, but she had some awsome ideas! Is it Friday yet? Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supv. Laboratory, Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3689 -----Original Message----- Subject: [Histonet] Special Stain "Storage" Someone out there has a clever idea and I'd like to tap that ingenuity. I have an overhead cabinet where I keep my special stains that don't require refrigeration. I'd like to keep each stain set together on the shelf - like in some kind of box. What do you use? One thing that comes to mind is sturdy plastic boxes that Lenscrafters, etc., use - roughly 9x6x3" (I've offered to take any extras but they are very fond of them and won't give 'em up!). Anyway, what do you use that keeps all the bottles together on the shelf? I'd love to have your input and I'll put you in my memoirs. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From debbiekeith <@t> cox.net Thu Nov 9 10:53:25 2006 From: debbiekeith <@t> cox.net (Debbie Keith) Date: Thu Nov 9 10:53:37 2006 Subject: [Histonet] i need used histo equipment... Message-ID: <5.2.0.9.0.20061109094908.02ebef28@pop.central.cox.net> Hi! I am trying to set up a (CHEAP) lab with used equipment. If anyone here has recently upgraded.. i would love to hear about your cast-offs!! i need: Shandon GLX Linistain Tissue Processor (enclosed) embedding center I appreciate any help! :) debbie -- No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.1.409 / Virus Database: 268.13.32/523 - Release Date: 11/7/2006 From froyer <@t> bitstream.net Thu Nov 9 10:55:58 2006 From: froyer <@t> bitstream.net (Ford Royer) Date: Thu Nov 9 10:56:35 2006 Subject: [Histonet] Off topic - Friday love story In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEF12D@sjhaexc02.sjha.org> Message-ID: <002801c7041f$ee1d66b0$7701a80a@Ford> Don't want to start a trend here, but here's a related tale regarding spousal death... Lena comes home from her twelve-hour shift at the Land O'Lakes Butter and Spreads plant only to find Ole at the kitchen table with tears in his eyes. "Min Gud Ole! Vat's ta mather vich ya?" asks Lena. "I vent ta da docktr ta day, unt he says I's gots only the 12 hours ta live." replies Ole. Lena says "Oh Ole, min love. Dat's da terrible nues. Vat vould I do ta make ta last hours spcial for ya, eh?" "Vell, I'd like ya to fex me min favort dinner of Lutefisk & Lefse" "Oh Ole, I'd been happy ta do tat." "Den I'd vant us ta go up ta bed and make love ALL ta nite long unt'll I passed avay." says Ole. "YA!" says Lena, "Tat's easy fo you'd ta say... you'd donna hav to get up and go ta vork in da mornin'!" ;-) Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. Golden Valley, MN ...where all the women are strong, all the men are good loking, and all the children are above average. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Thursday, November 09, 2006 10:15 AM To: Marshall Terry Dr,Consultant Histopathologist; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Off topic - Friday love story the fun has begun!!!! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Thursday, November 09, 2006 11:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Off topic - Friday love story I just had to send this - it is so moving. %%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% %%%% An elderly man lay dying in his bed. While suffering the agonies of impending death, he suddenly smelled the aroma of his favorite biscuits wafting up the stairs. He gathered his remaining strength, and lifted himself from the bed. Leaning on the wall, he slowly made his way out of the bedroom, and with even greater effort, gripping the railing with both hands, he crawled downstairs. With laboured breath, he leaned against the door-frame, gazing into the kitchen. Were it not for death's agony, he would have thought himself already in heaven, for there, spread out upon waxed paper on the kitchen table were literally hundreds of his favourite biscuits. Was it heaven? Or was it one final act of love from his devoted wife of sixty years, seeing to it that he left this world a happy man? Mustering one great final effort, he threw himself towards the table, landing on his knees in crumpled posture. His aged and withered hand trembled towards a biscuit at the edge of the table, when it was suddenly smacked by his wife with a spatula............. "F#ck off" she said, "They're for the funeral." Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From RSRICHMOND <@t> aol.com Thu Nov 9 10:59:54 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Thu Nov 9 11:00:10 2006 Subject: [Histonet] Re: processing possible TB infected tissue Message-ID: Jim Vickroy at Memorial Medical Center [where?] asks: >>We are reviewing our procedures and have a procedure that we really haven't been following. This is a procedure that calls for processing possible TB tissue by hand. This was based on an old report that possible TB bacteria could survive formalin fixation.?Right now we are processing all of our tissue by automated tissue processors, and we really only handle CJD tissue differently than the others. Obviously we don't have many cases suspected of TB but we do occasionally process granulomas that are suspicious for AFB positive organisms. The final diagnosis of these organisms is usually not known until long after the processing.<< Acid-fast bacilli are killed by formaldehyde fairly quickly, though as always cutting thin and fixing overnight provides an extra margin of safety. Hand processing of infected tissue is unnecessary. In at least half of cases a pathologist sees, the diagnosis of acid-fast disease wasn't suspected before the slides arrived. I think you should change your procedure. A question I've never gotten an answer to: is human tissue from AIDS patients with Mycobacterium avium-intracellulare (MAI) infection a satisfactory control for Mycobacterium tuberculosis stains? Prions in Creutzfeldt-Jakob disease and other prion diseases, as is well known, survive both formaldehyde and paraffin. Ordinary hospital pathology labs probably shouldn't process prion-infected tissue at all. I think that specialty labs do process it by hand. What are the veterinary labs' policies for bovine spongiform encephalopathy (mad cow disease) and chronic wasting disease of cervids? Bob Richmond Samurai Pathologist Knoxville TN From debbiekeith <@t> cox.net Thu Nov 9 11:10:09 2006 From: debbiekeith <@t> cox.net (Debbie Keith) Date: Thu Nov 9 11:10:21 2006 Subject: [Histonet] Special Stain "Storage" Message-ID: <5.2.0.9.0.20061109100945.00c1efc8@pop.central.cox.net> Terri & Shirley! I've been away from histonet for a while... i sort of "retired" a few years ago to be a stay-at-home mom. WOW! histology is so much easier than that job!!! :) i'm back a few days a week, now... and i guess i didn't realize how much i missed it. the stay-at-home gig made me a little crazy(er). i truly believe my histo experience sort of bled into my stay-at-home life... and made it much more organized. i have all sorts of histo-inspired organization in my house. (my kids have boxes that would rival your best special stain storage) and anyone old enough has a daily maintenance log. :) people that have seen it... think it's bizarre but my house is always about 15 minutes from being "mother-in-law-is-making-a-surprise-visit" ready!) one wonders... are most histotechs obsessive-compulsive as a RESULT of histo... OR did the OCD DRAW you TO histo. it's the age-old question... chicken or egg.... histo or OCD. :) -- No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.1.409 / Virus Database: 268.14.0/525 - Release Date: 11/9/2006 From gcallis <@t> montana.edu Thu Nov 9 11:28:01 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Nov 9 11:28:18 2006 Subject: [Histonet] Autofluorescence In-Reply-To: References: Message-ID: <6.0.0.22.1.20061109100129.01b3d8a8@gemini.msu.montana.edu> Stephen, I presume you are referring to the publication in J of Histochemistry Cytochemistry on this UV light exposure method to get rid of autofluorescence? I have seen a message on Histonet where they tried this and did not have success. I will be sending you a pdf on review of autofluorescence privately if you want it. Also, go to IHC World website, find discussions on fluorescence and autofluorescence - there are also other ways to attempt elimination of the problem. I did a copy/paste document from Histonet archives of a a superb discussion on ways to get rid of autofluorescence, and am forever grateful to the nice gentleman who put this together. Please excuse repetitive statements, it may have been composite of two messages(?). We use 100 mM glycine, in Dulbeccos PBS at 7.4 on rehydrated tissue sections, NOT frozen sections. With frozen sections we never prefix the tissue, they are cryosectioned in fresh state, then solvent fixed for immunofluorescence staining including anti GFP work (our fixative ruins the GFP) - this way we avoid any possiblity of aldehyde induced autofluorescence. Narrow band filters help as do spectral imaging instruments which can remove autofluorescence, as one can do on a confocal laser scanning microscope i.e. "gate it out" so to speak. Glycine method can also be used on a tissue coming out of fixative (soak in 100 mM glycine 1 hour, then rinse well with buffer, proceed to processing and maybe even before cryoprotection/snap freezing. We have not tried the latter. Your assessment on too thick sections is correct. ************************************************************************************************************************************* 1. After dehydrating your paraffin embedded slides and before blocking for protein incubate them in 100 mM glycine for 20 minutes. This will quench autofluorescence caused by free aldehydes. Works like a charm, I use it. 2. I've pretty much moved away from doing IF on paraffin embedded stuff(ICC yes) but I have had some experience trying to knock back autofluorescence (endogenous and fix related) in tissue sections. I've had some luck pre-treating fixed sections w/ one of the following: 1. 50mM Ammonium chloride in PBS for 10 min. 2. 0.1M Glycine in PBS, pH 7.4 for 5-10 min. 3. 1% Sodium borohydride in PBS for 10-20 min. It varies from sample to sample which method works the best but I've had the most success w/ the borohydride and NH4Cl methods. Autofluorescence can be brought on by certain endogenous tissue constituents, ie. fibronectin, lipofuscin and elastin, as well as by fixation in aldehydes. You don't say if your sections are fixed or not. If so, you should look at using sodium borohydride (0.5mg/ml in PBS) for 5 minutes (glutaraldehyde)or PBS plus a few drops of 1M glycine(formaldehyde) to block any reactive groups. ***Sodium borohydride is flammable on contact with water, and harmful by ingestion, inhalation etc. Take adequate precautions*** Another thing to consider is reducing the section thickness, if possible, as the intensity of autofluorescence is related to this. You also don't mention what fluorochromes you are using. It may be worthwhile trying a fluorochrome of a longer wavelength as there is less likelihood of any spectral overlap with the endogenous material. As I mentioned in an earlier posting today, we have had good results switching to the Alexa dyes (Molecular Probes). There are a couple of simple things you can do to help reduce autofluorescence. Some of the chemical reactions causing autofluorescence occur most rapidly with higher temperatures and on exposure to light. Therefore, performing the labeling at 4 C in the dark can help reduce this problem. Autofluorescence intensity is related to section thickness. You may want to try thinner sections if at all possible. Sometimes using fluorophores excited at longer wavelengths can help diminish autofluorescence. If autofluorescence is still an issue, there are a few preincubation steps you could try. A Tris-glycine mixture (adjust 0.1M glycine to pH 7.2-7.4 with 1M Tris base)will saturate free aldehyde groups. (15-30 minutes at room temp in Tris-glycine. Wash well in PBS The use of 1% sodium borohydride in PBS helps reduce any free aldehyde groups in the tissue, making them non-reactive. Incubate sections for 30 minutes in borohydride and then wash well(minimum 15 minutes) in several changes of PBS. Proceed with labeling. These techniques can be used alone or sequentially. If the tissue is fragile though, only use the Tris-glycine method. Please note that sodium borohydride is very reactive and is flammable on contact with water. Another technique to block unreacted groups is to incubate sections for 5 minutes in 50mM NH4Cl, and rinse in PBS before labeling. At 08:43 AM 11/9/2006, you wrote: >My name is Stephen Clark and i work in the neuro lab at Eastern Illinois >University. We use fluorescent immunohistochemistry to stain the >Olfactory Epithelium of mice and have recently had a problem with >autofluorescence of our tissues. They are fixed in 4% paraformaldehyde in >PBS and i have already tried sodium borohydrate with little success. I >have read about photobleaching using UV, neon, and lights of specific >wavelengths (488 and 633nm). I am just wondering if anyone utilizes >photobleaching via a light source and where it would be possible to >purchase them. I am also wondering if the 18um sections might be a little >too thick and whether that would have an increased affect on >autofluorescence. Any tips would be appreciated. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From oshel1pe <@t> cmich.edu Thu Nov 9 11:43:14 2006 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Thu Nov 9 11:43:31 2006 Subject: [Histonet] Special Stain "Storage" In-Reply-To: <5.2.0.9.0.20061109100945.00c1efc8@pop.central.cox.net> References: <5.2.0.9.0.20061109100945.00c1efc8@pop.central.cox.net> Message-ID: Hm. Are histotech anal-retentive? Is anal-retentive spelled with a hyphen or not? >Terri & Shirley! > >I've been away from histonet for a while... i sort of "retired" a >few years ago to be a stay-at-home mom. WOW! histology is so much >easier than that job!!! :) > >i'm back a few days a week, now... and i guess i didn't realize how >much i missed it. the stay-at-home gig made me a little crazy(er). > >i truly believe my histo experience sort of bled into my >stay-at-home life... and made it much more organized. i have all >sorts of histo-inspired organization in my house. (my kids have >boxes that would rival your best special stain storage) and anyone >old enough has a daily maintenance log. :) people that have seen >it... think it's bizarre but my house is always about 15 minutes >from being "mother-in-law-is-making-a-surprise-visit" ready!) > >one wonders... are most histotechs obsessive-compulsive as a RESULT >of histo... OR did the OCD DRAW you TO histo. it's the age-old >question... chicken or egg.... histo or OCD. > >:) -- Philip Oshel Microscopy Facility Supervisor Biology Department Central Michigan University 024C Brooks Hall Mt. Pleasant, MI 48859 voice: (989) 774-3576 dept. fax: (989) 774-3462 From rcharles <@t> state.pa.us Thu Nov 9 11:56:13 2006 From: rcharles <@t> state.pa.us (Charles, Roger) Date: Thu Nov 9 11:56:47 2006 Subject: [Histonet] Re: processing possible TB infected tissue Message-ID: <12E4E17FEF6EBE4BAE95BEB3CDCB57000943B9A6@enhbgpri04.backup> In the veterinary world we are using all dedicated equipment for all prion work. From forceps used to trim tissue to stainers doing the IHC work its all dedicated for prion work only. Twice the equipment, twice the cost. Roger Charles Microbiologist Pennsylvania Veterinary Laboratory 2305 N Cameron St Harrisburg, PA 17110 717-787-8808 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of RSRICHMOND@aol.com Sent: Thursday, November 09, 2006 12:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: processing possible TB infected tissue Jim Vickroy at Memorial Medical Center [where?] asks: >>We are reviewing our procedures and have a procedure that we really haven't been following. This is a procedure that calls for processing possible TB tissue by hand. This was based on an old report that possible TB bacteria could survive formalin fixation.?Right now we are processing all of our tissue by automated tissue processors, and we really only handle CJD tissue differently than the others. Obviously we don't have many cases suspected of TB but we do occasionally process granulomas that are suspicious for AFB positive organisms. The final diagnosis of these organisms is usually not known until long after the processing.<< Acid-fast bacilli are killed by formaldehyde fairly quickly, though as always cutting thin and fixing overnight provides an extra margin of safety. Hand processing of infected tissue is unnecessary. In at least half of cases a pathologist sees, the diagnosis of acid-fast disease wasn't suspected before the slides arrived. I think you should change your procedure. A question I've never gotten an answer to: is human tissue from AIDS patients with Mycobacterium avium-intracellulare (MAI) infection a satisfactory control for Mycobacterium tuberculosis stains? Prions in Creutzfeldt-Jakob disease and other prion diseases, as is well known, survive both formaldehyde and paraffin. Ordinary hospital pathology labs probably shouldn't process prion-infected tissue at all. I think that specialty labs do process it by hand. What are the veterinary labs' policies for bovine spongiform encephalopathy (mad cow disease) and chronic wasting disease of cervids? Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histology <@t> gradymem.org Thu Nov 9 12:02:28 2006 From: histology <@t> gradymem.org (histology@gradymem.org) Date: Thu Nov 9 12:02:43 2006 Subject: [Histonet] Special Stain "Storage" In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B0DB315@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B0DB315@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: I went to the dollar store and bought some clear plastic "shoe" boxes for about $1 apiece. I threw away the lid and put a sign on the end of the box and placed my bottles in them on the shelf. Works great. Make sure the boxes are not too long for your cabinet. Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org ----- Original Message ----- From: "Breeden, Sara" Date: Thursday, November 9, 2006 9:26 am Subject: [Histonet] Special Stain "Storage" To: histonet@lists.utsouthwestern.edu > Someone out there has a clever idea and I'd like to tap that > ingenuity.I have an overhead cabinet where I keep my special > stains that don't > require refrigeration. I'd like to keep each stain set together on the > shelf - like in some kind of box. What do you use? One thing that > comesto mind is sturdy plastic boxes that Lenscrafters, etc., use - > roughly > 9x6x3" (I've offered to take any extras but they are very fond of them > and won't give 'em up!). Anyway, what do you use that keeps all the > bottles together on the shelf? I'd love to have your input and > I'll put > you in my memoirs. > > > > Friday Hour of Fun is comin' up... > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gu.lang <@t> gmx.at Thu Nov 9 12:03:52 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Nov 9 12:03:12 2006 Subject: [Histonet] Special Stain "Storage" another question In-Reply-To: <01M9CVBILKFU8X3LFR@Macon2.Mercer.edu> Message-ID: <001e01c70429$6a841600$c812a8c0@dielangs.at> Hi all, Is there any regulation or good cause, that the special dye powders have to be stored in a safety-chemical-cabinet (I don't remember the right English term.)? We store our dyes in such a cabinet together with other chemicals. And the bottlecaps render ugly since then and get a white "beard". I would like to store them in a usual cupboard, nicely labelled and sortet alphabetically (like in the former years without the new safety-cabinet, where there were no such changes.). Gudrun Lang From Shirley_PHUA <@t> hsa.gov.sg Thu Nov 9 12:11:49 2006 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Thu Nov 9 12:12:10 2006 Subject: [Histonet] Shirley is away 10-17 November 2006 Message-ID: I will be out of the office from 10-11-2006 to 17-11-2006. I will return on 20 November 2006 Pathologists : I will process your requests when I return. Otherwise, if urgent, please forward your mail to henry_kyaw@hsa.gov.sg Regrets for the inconveniences caused. From tbraud <@t> holyredeemer.com Thu Nov 9 12:11:05 2006 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Thu Nov 9 12:12:47 2006 Subject: [Histonet] Special Stain "Storage" In-Reply-To: Message-ID: Just remember, you can't spell "analysis" without "anal" !!!! Terri -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Philip Oshel Sent: Thursday, November 09, 2006 12:43 PM To: Histonet@Pathology.swmed.edu Subject: RE: [Histonet] Special Stain "Storage" Hm. Are histotech anal-retentive? Is anal-retentive spelled with a hyphen or not? >Terri & Shirley! > >I've been away from histonet for a while... i sort of "retired" a >few years ago to be a stay-at-home mom. WOW! histology is so much >easier than that job!!! :) > >i'm back a few days a week, now... and i guess i didn't realize how >much i missed it. the stay-at-home gig made me a little crazy(er). > >i truly believe my histo experience sort of bled into my >stay-at-home life... and made it much more organized. i have all >sorts of histo-inspired organization in my house. (my kids have >boxes that would rival your best special stain storage) and anyone >old enough has a daily maintenance log. :) people that have seen >it... think it's bizarre but my house is always about 15 minutes >from being "mother-in-law-is-making-a-surprise-visit" ready!) > >one wonders... are most histotechs obsessive-compulsive as a RESULT >of histo... OR did the OCD DRAW you TO histo. it's the age-old >question... chicken or egg.... histo or OCD. > >:) -- Philip Oshel Microscopy Facility Supervisor Biology Department Central Michigan University 024C Brooks Hall Mt. Pleasant, MI 48859 voice: (989) 774-3576 dept. fax: (989) 774-3462 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. From algranth <@t> u.arizona.edu Thu Nov 9 12:43:04 2006 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Thu Nov 9 12:43:19 2006 Subject: [Histonet] Special Stain "Storage" In-Reply-To: References: Message-ID: <4.3.2.7.2.20061109113442.00cefe30@algranth.inbox.email.arizona.edu> I must have OCD - I put the date of purchase on my spices and other things like coffee and tea that are in the pantry and on baggies of fruit and veggies and meat in the freezer. I also have boxes of like things in the refrigerator and pantry. My grandmother used to do this and she wasn't even a histotech! Andi At 01:11 PM 11/9/2006 -0500, Terri Braud wrote: >Just remember, you can't spell "analysis" without "anal" !!!! >Terri > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Philip >Oshel >Sent: Thursday, November 09, 2006 12:43 PM >To: Histonet@Pathology.swmed.edu >Subject: RE: [Histonet] Special Stain "Storage" > > >Hm. Are histotech anal-retentive? >Is anal-retentive spelled with a hyphen or not? > > >Terri & Shirley! > > > >I've been away from histonet for a while... i sort of "retired" a > >few years ago to be a stay-at-home mom. WOW! histology is so much > >easier than that job!!! :) > > > >i'm back a few days a week, now... and i guess i didn't realize how > >much i missed it. the stay-at-home gig made me a little crazy(er). > > > >i truly believe my histo experience sort of bled into my > >stay-at-home life... and made it much more organized. i have all > >sorts of histo-inspired organization in my house. (my kids have > >boxes that would rival your best special stain storage) and anyone > >old enough has a daily maintenance log. :) people that have seen > >it... think it's bizarre but my house is always about 15 minutes > >from being "mother-in-law-is-making-a-surprise-visit" ready!) > > > >one wonders... are most histotechs obsessive-compulsive as a RESULT > >of histo... OR did the OCD DRAW you TO histo. it's the age-old > >question... chicken or egg.... histo or OCD. > > > >:) >-- >Philip Oshel >Microscopy Facility Supervisor >Biology Department >Central Michigan University >024C Brooks Hall >Mt. Pleasant, MI 48859 >voice: (989) 774-3576 >dept. fax: (989) 774-3462 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >CONFIDENTIALITY NOTICE: > >This E-Mail is intended only for the use of the individual or entity to which >it is addressed. It may contain information that is privileged and/or >confidential, >and the use or disclosure of such information may also be restricted under >applicable >federal and state law. If you received this communication in error, please >do not >distribute any part of it or retain any copies, and delete the original >E-Mail. >Please notify the sender of any error by E-Mail at the electronic address >shown. > >Thank you for your cooperation. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From petepath <@t> yahoo.com Thu Nov 9 12:57:13 2006 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Thu Nov 9 12:57:23 2006 Subject: [Histonet] Off topic - Friday hate story Message-ID: <922298.84642.qm@web30414.mail.mud.yahoo.com> I loved Dr Terry's story. Here is one to match. True story. A number of years back I was asked by one of our oncologists to review a case of an elderly woman who had been treated for high stage esophogeal carcinoma 9 years earlier. Given the theraputic responses of the day with her stage disease she was expected to have a very poor outcome. Nine years later she was still free of disease. The oncologist suspecting something might be wrong with the original interpretation wanted me to see if there was a mistake which could explain why she was still alive. There was no error. He mentioned to the patient that he was very surprised at her unusually good outcome and asked her if she had any explanation. She responded " I turned the hatred for my husband against the tumor" It is amazing the effect we can have on women! Stephen Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com From DBotsfor <@t> wrh.on.ca Thu Nov 9 12:59:03 2006 From: DBotsfor <@t> wrh.on.ca (Botsford, Daniel) Date: Thu Nov 9 13:03:45 2006 Subject: [Histonet] Table of contents Message-ID: <331EE6D48DDFD51189E500508BBD360806D98519@mail.wrh.on.ca> Hello Roxanne I hope the format "translates' okay. SECTION I- SPECIMEN HANDLING Cytology Specimen Handling Flow Chart his-i-01 1-1 Receipt of Specimens his-i-02 1-4 Referring Out Specimens his-i-03 1-3 Set-up for Gross Examination his-i-04 1-4 Shipment of Specimens his-i-05 1-3 IRREPLACABLE SPECIMEN Form HIS-I-06 1-1 CASSETTE ASSIGNMENT Chart HIS-I-07 1-4 section ii- tissue processing Processing Instruments his-ii-01 1-18 fixation his-ii-02 1-2 dehydration his-ii-03 1-3 clearing his-ii-04 1-2 wax impregnation his-ii-05 1-2 embedding his-ii-06 1-3 sectioning his-ii-07 1-3 coverslipping his-ii-08 1-3 labelling his-ii-09 1-2 Troubleshooting in processing his-ii-10 section iii- special procedures alcohol precurement his-iii-01 1-2 bile samples his-iii-02 1-2 chromosome studies his-iii-03 1-4 cjd- laboratory handling of tissue and csf his-iii-04 1-6 being reviewed crystal disease his-iii-05 1-3 decalcification his-iii-06 1-4 disposal of specimens his-iii-07 1-2 e.m. referral his-iii-08 1-2 estrogen receptors his-iii-09 1-3 expired antibodies his-iii-10 1-3 formalin Handling his-iii-11 1-2 frozen sections his-iii-12 1-4 her2/neu reporting his-iii-13 1-3 IHC AND SPECIAL STAINS CONTROLS HIS-III-14 1-2 koehler illumination his-iii-15 1-2 morgue procedures/fetal demise his-iii-16 1-2 muscle/kidney/nerve biopsy his-iii-17 1-5 reprocessing his-iii-18 1-2 sentinel lymph node his-iii-19 1-1 sick call tissue rescue his-iii-20 1-2 synovial & seminal fluids his-iii-21 1-4 DEPOSITION OF FETAL MATERIAL Form HIS-iii-22 1-1 specials stains controls Chart HIS-III-23 1-1 ihc controls Chart his-iii-24 1-2 MICROSCOPE MAINTENACE FORM(WRH-QAC-D-1) HIS-III-25 1-1 SECTION V -AUTOMATED IMMUNOPEROXIDASE STAINING HIS-V-01 1-15 IMMUNOFLUORESCENT STAINING HIS-V-02 1-3 RECYCLER HIS-V-03 1-4 ANTIBODIES/PROTOCOLS CHART HIS-V-04 1-2 RECYCLE FORM- XYLENE HIS-V-05 1-1 RECYCLE FORM- ALCOHOL HIS-V-06 1-1 SECTION VI- OPERATIONS CHEMICAL SPILLS HIS-OPS-01 1-2 CLEANING AND DECONTAMINATION OF STANDARD HIS-OPS-02 1-5 CLEAINING AND DISINFECTING EQUIPMENT BEFORE REPAIR OR MAINTENANCE HIS-OPS-03 1-5 CLEANING SCHEDULE HIS-OPS-04 1-2 HISTOLOGY UNITS HIS-OPS-05 1-3 INVENTORY AND ORDERING HIS-OPS-06 1-2 JOB SPECIFIC TRAINING HIS-OPS-07 1-1 MICROSCOPE MAINTENANCE HIS-OPS-08 1-2 MLA JOB TRAINING FORM HIS-OPS-09 1-3 MLT JOB TRAINING FORM HIS-OPS-10 1-9 PATHOLOGY SPECIMEN COLLECTION HIS-OPS-11 1-4 PATHOLOGY WORK DISTRIBUTION HIS-OPS-12 1-2 PROBLEM INVESTIGATION AND CORRECTIVE ACTION (See page 212 of QMS MANUAL) HIS-OPS-13 1-1 STORAGE OF SPECIMENS AND RECORD RETENTION (See pages 4-13 of OPERATION MANUAL) HIS-OPS-14 1-9 WORK ASSIGNMENTS HIS-OPS-15 1-1 QMP-LS PROCESS (See pages 187-189 of QMS MANUAL) HIS-OPS-16 1-3 WORK ASSIGNMENTS CHART HIS-OPS-17 1-1 PATHOLOGY WORK DISTRIBUTION FORM HIS-OPS-18 1-1 MICROSCOPE MAINTENANCE FORM HIS-OPS-19 1-1 DOCTORS UNITS CHART HIS-OPS-20 1-1 SECTION VII- MISCELLANOUS CYTOLOGY REGISTRY HIS-VII-01 1-1 DISINFECTION-GROSSROOM HIS-VII-02 1-1 DISINFECTION-HISTOLOGY HIS-VII-03 1-1 EMBEDDING FORM HIS-VII-04 1-1 EYE WASH STATION FORM HIS-VII-05 1-1 H&E STAINER FORM HIS-VII-06 1-1 INVENTORY-GENERAL FORM HIS-VII-07 1-1 INVENTORY- VENTANA FORM HIS-VII-08 1-1 REPROCESS/DELAY FORM HIS-VII-09 1-1 REPROCESS/FROZEN FORM HIS-VII-10 1-1 SPECIAL STAINS CHART HIS-VII-11 1-1 SURGICAL REGISTRY HIS-VII-12 1-1 TEMPERATURE FORM-GENERAL HIS-VII-13 1-1 TEMPERATURE FORM-VIP 200 HIS-VII-14 1-1 TEMPERATURE FORM-VIP 300 HIS-VII-15 1-1 TEMPERATURE FORM-VIP 5 HIGH HIS-VII-16 1-1 TEMPERATURE FORM-VIP 5 LOW HIS-VII-17 1-1 BULK REAGENT DECONTAMINATION HIS-VII-18 1-1 COVERSLIPPER FORM HIS-VII-19 1-1 SEND OUTS HIS-VII-20 1-1 Sincerely Daniel Botsford Windsor Regional Hospital 1995 Lens Avenue Windsor, Ontario N8W 1L9 519-254-5577 ext 52373 519-254-6861 fax dbotsfor@wrh.on.ca Message: 6 Date: Thu, 09 Nov 2006 11:34:13 -0500 From: godsgalnow@aol.com Subject: [Histonet] TABLE OF CONTENTS To: histonet@lists.utsouthwestern.edu Message-ID: <8C8D23406BA318F-76C-797B@WEBMAIL-MA17.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Does anyone out there have a table of contents to their procedure manual that they are willing to share? I would greatly appreciate it......... Roxanne Soto HT(ASCP)QIHC Lab Manager PhysiciansRightPath Tamap, FL 813-549-1050 From TJJ <@t> Stowers-Institute.org Thu Nov 9 13:10:09 2006 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Thu Nov 9 13:10:32 2006 Subject: [Histonet] How efficient is formalin in killing HIV and Hepatitis? Message-ID: Does anybody have published guidelines on how much time it takes for formalin (10% NBF) to effectively render HIV and Hepatitis non-infective? Thanks for your help! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From dmccaig <@t> ckha.on.ca Thu Nov 9 13:19:09 2006 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Thu Nov 9 13:19:22 2006 Subject: [Histonet] (no subject) Message-ID: -Does anyone know where I can acquire an Anglia Scientific Microtome Belt---Model number AS? Mine just broke. Thanks Diana McCaig Chatham Kent Health Alliance 519-352-6401 ext 6604 From Jackie.O'Connor <@t> abbott.com Thu Nov 9 13:22:48 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Nov 9 13:23:24 2006 Subject: [Histonet] Special Stain "Storage" In-Reply-To: <4.3.2.7.2.20061109113442.00cefe30@algranth.inbox.email.arizona.edu> Message-ID: Hmmmm . . . .I have the opposite problem. Every once in awhile, one of my kids will call me from the kitchen and ask, "Mom - are these green pork chops in the refrigerator still good?" Andrea Grantham Sent by: histonet-bounces@lists.utsouthwestern.edu 11/09/2006 12:43 PM To "Histonet (E-mail)" cc Subject RE: [Histonet] Special Stain "Storage" I must have OCD - I put the date of purchase on my spices and other things like coffee and tea that are in the pantry and on baggies of fruit and veggies and meat in the freezer. I also have boxes of like things in the refrigerator and pantry. My grandmother used to do this and she wasn't even a histotech! Andi At 01:11 PM 11/9/2006 -0500, Terri Braud wrote: >Just remember, you can't spell "analysis" without "anal" !!!! >Terri > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Philip >Oshel >Sent: Thursday, November 09, 2006 12:43 PM >To: Histonet@Pathology.swmed.edu >Subject: RE: [Histonet] Special Stain "Storage" > > >Hm. Are histotech anal-retentive? >Is anal-retentive spelled with a hyphen or not? > > >Terri & Shirley! > > > >I've been away from histonet for a while... i sort of "retired" a > >few years ago to be a stay-at-home mom. WOW! histology is so much > >easier than that job!!! :) > > > >i'm back a few days a week, now... and i guess i didn't realize how > >much i missed it. the stay-at-home gig made me a little crazy(er). > > > >i truly believe my histo experience sort of bled into my > >stay-at-home life... and made it much more organized. i have all > >sorts of histo-inspired organization in my house. (my kids have > >boxes that would rival your best special stain storage) and anyone > >old enough has a daily maintenance log. :) people that have seen > >it... think it's bizarre but my house is always about 15 minutes > >from being "mother-in-law-is-making-a-surprise-visit" ready!) > > > >one wonders... are most histotechs obsessive-compulsive as a RESULT > >of histo... OR did the OCD DRAW you TO histo. it's the age-old > >question... chicken or egg.... histo or OCD. > > > >:) >-- >Philip Oshel >Microscopy Facility Supervisor >Biology Department >Central Michigan University >024C Brooks Hall >Mt. Pleasant, MI 48859 >voice: (989) 774-3576 >dept. fax: (989) 774-3462 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >CONFIDENTIALITY NOTICE: > >This E-Mail is intended only for the use of the individual or entity to which >it is addressed. It may contain information that is privileged and/or >confidential, >and the use or disclosure of such information may also be restricted under >applicable >federal and state law. If you received this communication in error, please >do not >distribute any part of it or retain any copies, and delete the original >E-Mail. >Please notify the sender of any error by E-Mail at the electronic address >shown. > >Thank you for your cooperation. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From debbiekeith <@t> cox.net Thu Nov 9 13:29:37 2006 From: debbiekeith <@t> cox.net (Debbie Keith) Date: Thu Nov 9 13:29:49 2006 Subject: [Histonet] Special Stain "Storage" In-Reply-To: References: <4.3.2.7.2.20061109113442.00cefe30@algranth.inbox.email.arizona.edu> Message-ID: <5.2.0.9.0.20061109122906.05146fb8@pop.central.cox.net> are you trying to make your own control tissue? ;) At 01:22 PM 11/9/2006 -0600, you wrote: >Hmmmm . . . .I have the opposite problem. Every once in awhile, one of >my kids will call me from the kitchen and ask, "Mom - are these green pork >chops in the refrigerator still good?" > > > > > >Andrea Grantham >Sent by: histonet-bounces@lists.utsouthwestern.edu >11/09/2006 12:43 PM -- No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.1.409 / Virus Database: 268.14.0/525 - Release Date: 11/9/2006 From sbreeden <@t> nmda.nmsu.edu Thu Nov 9 13:31:54 2006 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Nov 9 13:32:02 2006 Subject: [Histonet] Storage Solutions Thanks! Message-ID: <4D14F0FC9316DD41972D5F03C070908B0DB321@nmdamailsvr.nmda.ad.nmsu.edu> Thank you to EVERYONE that responded with those priceless-but-little-known tips on how to store special stain components all in one place! I will be haunting the Dollar Stores, Home Depot, Lowe's, Wally World, etc. for days (Beat me! Kick me! Make me go shopping!!). It's good to liven up a Thursday-before-a-Friday like this. I know I don't usually get real serious with my postings, but I watch Histonet like a hawk and I glean valuable information. However, we all need to have a little chuckle once in a while. I have a Project for the Friday Hour of Fun - it's not so much a "joke" as it is a chance to get creative... Joe, if you're out there, we miss you. Put up a smoke signal or something... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 From gentras <@t> vetmed.auburn.edu Thu Nov 9 14:05:42 2006 From: gentras <@t> vetmed.auburn.edu (Atoska Gentry) Date: Thu Nov 9 14:06:03 2006 Subject: [Histonet] x-gal protocol Message-ID: <45538A16.2070101@vetmed.auburn.edu> Hello Rob, I'm wondering if you'd be so kind as to share with me your x-gal staining procedure ASAP? I have a colleague who's complaining about losing the majority of her x-gal staining on frozen brain sections. She said the staining looks great until she reaches xylene, then a fair amount disappears. She said that she didn't use a counterstain and her supervisor feels a counterstain will aide in better viewing of the staining results. I recently came across a reply you made to a Beth Roche in a similar situation by way of histonet archives. However, your protocol was not shown. Your prompt reply will be much appreciated. Atoska -- Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu From NMargaryan <@t> childrensmemorial.org Thu Nov 9 14:24:41 2006 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Thu Nov 9 14:19:55 2006 Subject: [Histonet] FW: Joke Message-ID: <63B8B599DE283148B92E83C78B32C15D03680F6A@cmhexbe2.childrensmemorial.org> Here is a joke to laugh at!! Subject: FW: Joke TWO OLD MEN DECIDE THEY ARE CLOSE TO THEIR LAST DAYS AND DECIDE TO HAVE A LAST NIGHT ON THE TOWN. AFTER A FEW DRINKS, THEY END UP AT THE LOCAL BROTHEL. THE MADAM TAKES ONE LOOK AT THE TWO OLD GEEZERS AND WHISPERS TO HER MANAGER, "GO UP TO THE FIRST TWO BEDROOMS AND PUT AN INFLATED DOLL IN EACH BED. THESE TWO ARE SO OLD AND DRUNK, I'M NOT WASTING TWO OF MY GIRLS ON THEM. THEY WON'T KNOW THE DIFFERENCE." THE MANAGER DOES AS HE IS TOLD AND THE TWO OLD MEN GO UPSTAIRS AND TAKE CARE OF THEIR BUSINESS. AS THEY ARE WALKING HOME THE FIRST MAN SAYS, "YOU KNOW, I THINK MY GIRL WAS DEAD!" "DEAD?" SAYS HIS FRIEND, "WHY DO YOU SAY THAT?" "WELL, SHE NEVER MOVED OR MADE A SOUND ALL THE TIME I WAS LOVING HER." HIS FRIEND SAYS, "COULD BE WORSE I THINK MINE WAS A WITCH." "A WITCH, WHY THE HELL WOULD YOU SAY THAT?" "WELL, I WAS MAKING LOVE TO HER, KISSING HER ON THE NECK AND I GAVE HER A LITTLE BITE, THEN SHE FARTED AND FLEW OUT THE WINDOW. HAHAHAHAHA!!!! From Janet.Bonner <@t> FLHosp.org Thu Nov 9 14:24:57 2006 From: Janet.Bonner <@t> FLHosp.org (Bonner, Janet) Date: Thu Nov 9 14:25:39 2006 Subject: [Histonet] Special Stain "Storage" References: <4.3.2.7.2.20061109113442.00cefe30@algranth.inbox.email.arizona.edu> Message-ID: <5F31F38C96781A4FBE3196EBC22D478004A69B@fhosxchmb006.ADVENTISTCORP.NET> But she very well could have been! @:) ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Andrea Grantham Sent: Thu 11/9/2006 1:43 PM To: Histonet (E-mail) Subject: RE: [Histonet] Special Stain "Storage" I must have OCD - I put the date of purchase on my spices and other things like coffee and tea that are in the pantry and on baggies of fruit and veggies and meat in the freezer. I also have boxes of like things in the refrigerator and pantry. My grandmother used to do this and she wasn't even a histotech! Andi At 01:11 PM 11/9/2006 -0500, Terri Braud wrote: >Just remember, you can't spell "analysis" without "anal" !!!! >Terri > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Philip >Oshel >Sent: Thursday, November 09, 2006 12:43 PM >To: Histonet@Pathology.swmed.edu >Subject: RE: [Histonet] Special Stain "Storage" > > >Hm. Are histotech anal-retentive? >Is anal-retentive spelled with a hyphen or not? > > >Terri & Shirley! > > > >I've been away from histonet for a while... i sort of "retired" a > >few years ago to be a stay-at-home mom. WOW! histology is so much > >easier than that job!!! :) > > > >i'm back a few days a week, now... and i guess i didn't realize how > >much i missed it. the stay-at-home gig made me a little crazy(er). > > > >i truly believe my histo experience sort of bled into my > >stay-at-home life... and made it much more organized. i have all > >sorts of histo-inspired organization in my house. (my kids have > >boxes that would rival your best special stain storage) and anyone > >old enough has a daily maintenance log. :) people that have seen > >it... think it's bizarre but my house is always about 15 minutes > >from being "mother-in-law-is-making-a-surprise-visit" ready!) > > > >one wonders... are most histotechs obsessive-compulsive as a RESULT > >of histo... OR did the OCD DRAW you TO histo. it's the age-old > >question... chicken or egg.... histo or OCD. > > > >:) >-- >Philip Oshel >Microscopy Facility Supervisor >Biology Department >Central Michigan University >024C Brooks Hall >Mt. Pleasant, MI 48859 >voice: (989) 774-3576 >dept. fax: (989) 774-3462 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >CONFIDENTIALITY NOTICE: > >This E-Mail is intended only for the use of the individual or entity to which >it is addressed. It may contain information that is privileged and/or >confidential, >and the use or disclosure of such information may also be restricted under >applicable >federal and state law. If you received this communication in error, please >do not >distribute any part of it or retain any copies, and delete the original >E-Mail. >Please notify the sender of any error by E-Mail at the electronic address >shown. > >Thank you for your cooperation. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From funderwood <@t> mcohio.org Thu Nov 9 14:31:04 2006 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Thu Nov 9 14:31:35 2006 Subject: [Histonet] Special Stain "Storage" Message-ID: That's not just a meal, but gram control material as well. >>> "Jackie M O'Connor" 11/9/2006 2:22 PM >>> Hmmmm . . . .I have the opposite problem. Every once in awhile, one of my kids will call me from the kitchen and ask, "Mom - are these green pork chops in the refrigerator still good?" Andrea Grantham Sent by: histonet-bounces@lists.utsouthwestern.edu 11/09/2006 12:43 PM To "Histonet (E-mail)" cc Subject RE: [Histonet] Special Stain "Storage" I must have OCD - I put the date of purchase on my spices and other things like coffee and tea that are in the pantry and on baggies of fruit and veggies and meat in the freezer. I also have boxes of like things in the refrigerator and pantry. My grandmother used to do this and she wasn't even a histotech! Andi At 01:11 PM 11/9/2006 -0500, Terri Braud wrote: >Just remember, you can't spell "analysis" without "anal" !!!! >Terri > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Philip >Oshel >Sent: Thursday, November 09, 2006 12:43 PM >To: Histonet@Pathology.swmed.edu >Subject: RE: [Histonet] Special Stain "Storage" > > >Hm. Are histotech anal-retentive? >Is anal-retentive spelled with a hyphen or not? > > >Terri & Shirley! > > > >I've been away from histonet for a while... i sort of "retired" a > >few years ago to be a stay-at-home mom. WOW! histology is so much > >easier than that job!!! :) > > > >i'm back a few days a week, now... and i guess i didn't realize how > >much i missed it. the stay-at-home gig made me a little crazy(er). > > > >i truly believe my histo experience sort of bled into my > >stay-at-home life... and made it much more organized. i have all > >sorts of histo-inspired organization in my house. (my kids have > >boxes that would rival your best special stain storage) and anyone > >old enough has a daily maintenance log. :) people that have seen > >it... think it's bizarre but my house is always about 15 minutes > >from being "mother-in-law-is-making-a-surprise-visit" ready!) > > > >one wonders... are most histotechs obsessive-compulsive as a RESULT > >of histo... OR did the OCD DRAW you TO histo. it's the age-old > >question... chicken or egg.... histo or OCD. > > > >:) >-- >Philip Oshel >Microscopy Facility Supervisor >Biology Department >Central Michigan University >024C Brooks Hall >Mt. Pleasant, MI 48859 >voice: (989) 774-3576 >dept. fax: (989) 774-3462 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >CONFIDENTIALITY NOTICE: > >This E-Mail is intended only for the use of the individual or entity to which >it is addressed. It may contain information that is privileged and/or >confidential, >and the use or disclosure of such information may also be restricted under >applicable >federal and state law. If you received this communication in error, please >do not >distribute any part of it or retain any copies, and delete the original >E-Mail. >Please notify the sender of any error by E-Mail at the electronic address >shown. > >Thank you for your cooperation. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tthinnes <@t> scripps.edu Thu Nov 9 17:11:08 2006 From: tthinnes <@t> scripps.edu (terri thinnes) Date: Thu Nov 9 17:11:12 2006 Subject: [Histonet] microtome purchase Message-ID: <000001c70454$57462fd0$21d48389@innateimmunweb> Dear Histonetters, any ideas on whether or not this is a good purchase? Microm microtome 2002 (used) HM 335E? Thanks for any suggestions. From SHARON.OSBORN <@t> SPCORP.COM Thu Nov 9 19:20:45 2006 From: SHARON.OSBORN <@t> SPCORP.COM (Osborn, Sharon) Date: Thu Nov 9 19:20:57 2006 Subject: [Histonet] Train To End Stroke Message-ID: <9A919A5D70313A4D9C56A02571087408C5452D@kenmsg40.us.schp.com> Histonetters! I request your help in reaching my goal to raise $3,000 by December 1st. I am participating in the Orange County CA Marathon and Half Marathon in January 2007. I am walking the half marathon. This is a benefit for the American Stroke Association. Have you experienced stroke or know someone who has? One in 5 people will experience stroke. Most occur before age 65. Every 45 seconds someone suffers a stroke while every 3.1 minutes some dies of a stroke. It is the most debilitating disease we have. My mother died Oct 23rd of her second stroke in 45 days. She is the 4th sibling to die from stroke complications. Have you lost someone to stroke? This money goes for research into prevention, causes and treatment of stroke as well as education. I appreciate any donations you make. You can do it in one of two ways. You can ask me back channel for an address to send your checks or money orders to...or you can go directly online to make credit card donations. My website is http://bayarea.kintera.org/oc2006/sosborn I am excited about doing my first (half) marathon! And, I am excited to be doing it for Train To End Stroke through the American Stroke Association! If you have someone you wish to donate in memory of, please fill that in online or let me know. I am happy to add them to my list of heroes/memories I am walking for. Sharon Osborn SP BioPharma Palo Alto, CA 650.496.6539 ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From RSRICHMOND <@t> aol.com Thu Nov 9 22:23:44 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Thu Nov 9 22:23:51 2006 Subject: [Histonet] Re: Cut resistant gloves Message-ID: Kemlo Rogerson advises us to get Kevlar (aramid) gloves at >>Motorbike shops; I've got some. Really kewl looking.<< (Kemlo's in the UK.) I went to the nearest Harley Davidson shop, looked at some really remarkable Hawg donorcycles, and was referred to a remarkably seamy military supply shop (see greenmilitary.com) where I found numerous styles of Kevlar gloves to protect the dorsal surfaces of the hands (don't ask). I then looked up >>kevlar gloves<< in Google and found that you can indeed get all-Kevlar gloves, even from Fisher Scientific. It's odd there's been so little interest in them. Bob Richmond Samurai Pathologist Knoxville TN From muddymoo <@t> gmail.com Thu Nov 9 23:29:18 2006 From: muddymoo <@t> gmail.com (Alan Bishop) Date: Thu Nov 9 23:29:27 2006 Subject: [Histonet] Eridan news? Message-ID: Anyone heard any further news on the Dako Eridan? Have heard today that the machines that were in labs have been withdrawn but not been able to get confirmation of this? Looks like it might be the end of the orad for the mythical new machine. Anyone heard anything?? Cheers A From patrick.howorth <@t> bristol.ac.uk Fri Nov 10 03:13:05 2006 From: patrick.howorth <@t> bristol.ac.uk (PW Howorth, Physiology) Date: Fri Nov 10 03:13:35 2006 Subject: [Histonet] Re: Antibody problems Message-ID: Hi folks, Recently, I have been enduring a problem which is undoubtedly familiar to some and I was wondering how have you have overcome the dilemma that I'm facing. The problem is that I need to do some double labeling, however, the current antibodies that I use are derived in rabbit and in both methods, I use a biotinylated secondary step, followed by strept-avidin Cy3. The obvious step is to use different species with conjugated fluorophore secondary, but having spent 2 months in frustration, I am willing to consider other options, namely; FAB fragments to change 1 antibody species from rabbit to say, goat; and avidin-D kit to block endogenous biotin (but in this case, it would be spare biotin sites remaining from first antibody detection). Do any of you good folks, use either of these methods or have tried them and would be willing to share your thoughts ? Many thanks Patrick ---------------------- Patrick Howorth, Dept of Physiology, University of Bristol, University Walk, Bristol, Avon. BS8 1TD. +44 (0)117 33 17112 (office) +44 (0)117 33 17579 (lab) patrick.howorth@bristol.ac.uk From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Nov 10 04:02:09 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Nov 10 04:01:17 2006 Subject: [Histonet] Re: Cut resistant gloves Message-ID: <86ADE4EB583CE64799A9924684A0FBBFAFC92C@wahtntex2.waht.swest.nhs.uk> Harley Davidson? Oh I see those big old bikes that are VERY VERY SLOW and don't go round corners. No I meant real bikes, Yamaha, Suzuki, Honda, good british bikes!!!! Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Habits of thinking need not be forever. One of the most significant findings in psychology in the last twenty years is that individuals can choose the way they think. --Martin Seligman This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From BMolinari <@t> heart.thi.tmc.edu Fri Nov 10 05:31:23 2006 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Nov 10 05:31:28 2006 Subject: [Histonet] FW: Joke In-Reply-To: <63B8B599DE283148B92E83C78B32C15D03680F6A@cmhexbe2.childrensmemorial.org> Message-ID: Oh, it is good to laugh to start off a Friday! Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Margaryan, Naira Sent: Thursday, November 09, 2006 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Joke Here is a joke to laugh at!! Subject: FW: Joke TWO OLD MEN DECIDE THEY ARE CLOSE TO THEIR LAST DAYS AND DECIDE TO HAVE A LAST NIGHT ON THE TOWN. AFTER A FEW DRINKS, THEY END UP AT THE LOCAL BROTHEL. THE MADAM TAKES ONE LOOK AT THE TWO OLD GEEZERS AND WHISPERS TO HER MANAGER, "GO UP TO THE FIRST TWO BEDROOMS AND PUT AN INFLATED DOLL IN EACH BED. THESE TWO ARE SO OLD AND DRUNK, I'M NOT WASTING TWO OF MY GIRLS ON THEM. THEY WON'T KNOW THE DIFFERENCE." THE MANAGER DOES AS HE IS TOLD AND THE TWO OLD MEN GO UPSTAIRS AND TAKE CARE OF THEIR BUSINESS. AS THEY ARE WALKING HOME THE FIRST MAN SAYS, "YOU KNOW, I THINK MY GIRL WAS DEAD!" "DEAD?" SAYS HIS FRIEND, "WHY DO YOU SAY THAT?" "WELL, SHE NEVER MOVED OR MADE A SOUND ALL THE TIME I WAS LOVING HER." HIS FRIEND SAYS, "COULD BE WORSE I THINK MINE WAS A WITCH." "A WITCH, WHY THE HELL WOULD YOU SAY THAT?" "WELL, I WAS MAKING LOVE TO HER, KISSING HER ON THE NECK AND I GAVE HER A LITTLE BITE, THEN SHE FARTED AND FLEW OUT THE WINDOW. HAHAHAHAHA!!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Fri Nov 10 07:05:49 2006 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Nov 10 07:05:56 2006 Subject: [Histonet] Friday Fun Message-ID: <4D14F0FC9316DD41972D5F03C070908B0DB329@nmdamailsvr.nmda.ad.nmsu.edu> Okay, here's the deal. Tell me, concisely, what would be the most unlikely thing a histologist would hear from a pathologist? For example, "No, please! Don't recut that broken slide - I can see just fine through the cellophane tape". Send your innovative, imaginative, and funny responses directly to me at sbreeden@nmda.nmsu.edu. This could lead to a Very Funny Friday! "Start your engines!!" Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 From haldana <@t> unimoron.edu.ar Thu Nov 9 09:45:39 2006 From: haldana <@t> unimoron.edu.ar (Hernan Aldana) Date: Fri Nov 10 07:24:12 2006 Subject: [Histonet] Help with immunohistochemistry in human embryos In-Reply-To: <200610011403398.SM01224@swlx162.swmed.edu> Message-ID: <004d01c70416$1b5b7a40$5063e7c9@ARIEL> Dear Histoneters I need some advices. I'm working with human embryos between 7 and 14 weeks. we used Sonic Hedgehog Shh (C-18) and sc-1195 antibodies of Santa Cruz Biotechnology in paraffin sections of embryos fixed in paraformadehyde. I don't have good results. Could anybody help me with some advice, concerning to every step of my technique? Thanks in advance Dr. Hern?n Javier Aldana Marcos Prof. Titular Reg. Histolog?a, Embriolog?a y Biolog?a Celular Facultad de Medicina Universidad de Mor?n Argentina email altenrativo: hernanjavier@yahoo.com http://www.ht.org.ar/educacion.htm -----Mensaje original----- De: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] En nombre de histonet-request@lists.utsouthwestern.edu Enviado el: Domingo, 01 de Octubre de 2006 02:04 p.m. Para: histonet@lists.utsouthwestern.edu Asunto: Histonet Digest, Vol 35, Issue 1 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Job Opening (Luck, Greg D.) 2. RE: Problem of Double immunoflouresence staining .. (Melissa Gonzalez) 3. The procedure that substitues xylene with mineral oil. (PhiHo Hoang) 4. Re: The procedure that substitues xylene with mineral oil. (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Sat, 30 Sep 2006 12:01:02 -0700 From: "Luck, Greg D." Subject: [Histonet] Job Opening To: Cc: "Ringwood, James F." , "Wakabayashi, Marlene T." , "Browning, Rachel M." Message-ID: <6BB8BC4519AAB844B174FC739A679BBC50900C@IRMEXCH01.irm.inhs.org> Content-Type: text/plain; charset="us-ascii" Hello All, Deaconess is a 300 bed acute care community medical center. We have a full time, M-F, day time position open. Must be HT(ASCP) certified or eligible. Duties include all routine histology functions in a clinical setting (i.e. embedding , cutting, special stains and frozen sections). IHC experience preferred. Due to a recent market adjustment by our organization the salary range for this position has just increased 25-33% depending on years of experience. This is a golden opportunity for someone to improve on their current situation or to begin anew. Anyone new to this field is encouraged to apply as well, for the door is wide open. You can apply on-line by going to our corporate website www.empirehealth.org. The position is at Deaconess Medical Center in Spokane, WA www.deaconess-spokane.org. Please contact me directly for more details. Following is an additional web link to provide you with more info on Spokane and the greater Inland Northwest region www.visitspokane.com. ("Near Perfect-Near Nature"). Thank you and best wishes, Greg Greg Luck, BS, HT(ASCP) Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 Phone 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconess-spokane.org ------------------------------ Message: 2 Date: Sun, 1 Oct 2006 08:40:44 -0700 From: "Melissa Gonzalez" Subject: [Histonet] RE: Problem of Double immunoflouresence staining .. To: Message-ID: Content-Type: text/plain; charset="us-ascii" Sohail, First, Perhaps you should try incubating each primary with respective secondary only, to determine how they look separately, and also make sure each is working on its own. For example, you may not have your aSMA dilution correct. Also, do you have a positive control for TRITC to make sure you can detect any TRITC signal adequately? Once you have appropriate signal from both, you can then make your cocktails. Also, in the future, I would recommend switching to Alexa Fluors from Molecular Probes, they really are great. In your instance you would use AF488 (FITC) and AF 546 or 555 (TRITC). But that is just my 2 cents. Good luck, Melissa Message: 4 Date: Fri, 29 Sep 2006 13:06:27 -0700 (PDT) From: sohail ejaz Subject: [Histonet] Problem of Double immunoflouresence staining of retinal vasculature To: histonet@lists.utsouthwestern.edu Message-ID: <20060929200627.949.qmail@web39506.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello everybody I have a big problem of staining retina vasculature using a cocktail of two different antibodies. The problem is that i can only see staining with FITC but not with TRITC, hence i cant make a merger of both FITC and TRITC. Cocktail of primary antibodies include (1) Monoclonal Rabbit Anti human vWF (2) Monoclonal mouse Anti alpha SMA and cocktail of secondary antibodies include (1) Anti rabbit FITC (2) Anti mouse TRITC Please let me know your commentc to slove this issue. Dr.Sohail ------------------------------ Message: 3 Date: Sun, 1 Oct 2006 11:47:56 -0400 From: "PhiHo Hoang" Subject: [Histonet] The procedure that substitues xylene with mineral oil. To: Message-ID: <005b01c6e570$f725b820$f300a8c0@ttth> Content-Type: text/plain; charset="iso-8859-1" Greetings, I am a new subscriber to this list and I am reading the list archive. I came across a posting from Reni J. responding to a request from Sharon: Sharon: I am sending privately a procedure I have that substitutes xylene with mineral oil. It is 100 times less toxic than xylene and 3 times cheaper. Reni J. Sharon Allen exchange.hsc.mb.ca> wrote: Does anyone use xylol substitutes, if so how good are they? We do CJD testing & "the powers that be" don't like to pay for getting rid of contaminated xylol. I don't want to have to start testing different substitutes, so any help would be greatly appreciated. Thanks Sharon sallen <@t> hsc.mb.ca I am interested in this procedure. It is very much appreciated if I can get a copy of that procedure. Best regards, PhiHo ------------------------------ Message: 4 Date: Sun, 1 Oct 2006 09:55:53 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] The procedure that substitues xylene with mineral oil. To: PhiHo Hoang , histonet@lists.utsouthwestern.edu Message-ID: <20061001165553.32345.qmail@web61219.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 PhiHo: I am attaching the procedure under separate cover. Reni J. PhiHo Hoang wrote: Greetings, I am a new subscriber to this list and I am reading the list archive. I came across a posting from Reni J. responding to a request from Sharon: Sharon: I am sending privately a procedure I have that substitutes xylene with mineral oil. It is 100 times less toxic than xylene and 3 times cheaper. Reni J. Sharon Allen exchange.hsc.mb.ca> wrote: Does anyone use xylol substitutes, if so how good are they? We do CJD testing & "the powers that be" don't like to pay for getting rid of contaminated xylol. I don't want to have to start testing different substitutes, so any help would be greatly appreciated. Thanks Sharon sallen <@t> hsc.mb.ca I am interested in this procedure. It is very much appreciated if I can get a copy of that procedure. Best regards, PhiHo _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1"/min. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 35, Issue 1 *************************************** From JMahoney <@t> alegent.org Fri Nov 10 07:51:21 2006 From: JMahoney <@t> alegent.org (Janice Mahoney) Date: Fri Nov 10 07:51:42 2006 Subject: [Histonet] Friday Fun Message-ID: How about "I don't want my slides until noon". Jan, Omaha NE (go Huskers!) >>> "Breeden, Sara" 11/10/2006 7:05 AM >>> Okay, here's the deal. Tell me, concisely, what would be the most unlikely thing a histologist would hear from a pathologist? For example, "No, please! Don't recut that broken slide - I can see just fine through the cellophane tape". Send your innovative, imaginative, and funny responses directly to me at sbreeden@nmda.nmsu.edu. This could lead to a Very Funny Friday! "Start your engines!!" Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Fri Nov 10 08:03:43 2006 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri Nov 10 08:03:53 2006 Subject: [Histonet] Friday Fun In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B0DB329@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: No problem, I'll wait until the mounting media has set before I look at the slide under oil immersion. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: 10 November 2006 13:06 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Friday Fun Okay, here's the deal. Tell me, concisely, what would be the most unlikely thing a histologist would hear from a pathologist? For example, "No, please! Don't recut that broken slide - I can see just fine through the cellophane tape". Send your innovative, imaginative, and funny responses directly to me at sbreeden@nmda.nmsu.edu. This could lead to a Very Funny Friday! "Start your engines!!" Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Fri Nov 10 08:22:56 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Nov 10 08:26:46 2006 Subject: [Histonet] Friday Fun References: <4D14F0FC9316DD41972D5F03C070908B0DB329@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <5F31F38C96781A4FBE3196EBC22D478004A6A0@fhosxchmb006.ADVENTISTCORP.NET> "I want one patient case per folder (20 slide spaces per folder) even if there's only one slide on the case." -We process at least 300 cases, 1500 slides, per day. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Breeden, Sara Sent: Fri 11/10/2006 8:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Friday Fun Okay, here's the deal. Tell me, concisely, what would be the most unlikely thing a histologist would hear from a pathologist? For example, "No, please! Don't recut that broken slide - I can see just fine through the cellophane tape". Send your innovative, imaginative, and funny responses directly to me at sbreeden@nmda.nmsu.edu. This could lead to a Very Funny Friday! "Start your engines!!" Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From JWEEMS <@t> sjha.org Fri Nov 10 08:38:19 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Nov 10 08:38:39 2006 Subject: [Histonet] Friday Fun Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEF14F@sjhaexc02.sjha.org> "There is really no need for a tech to work Saturdays any more. We'll just have someone on call." -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Breeden, Sara Sent: Friday, November 10, 2006 8:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Friday Fun Okay, here's the deal. Tell me, concisely, what would be the most unlikely thing a histologist would hear from a pathologist? For example, "No, please! Don't recut that broken slide - I can see just fine through the cellophane tape". Send your innovative, imaginative, and funny responses directly to me at sbreeden@nmda.nmsu.edu. This could lead to a Very Funny Friday! "Start your engines!!" Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From ASenn <@t> mercy.pmhs.org Fri Nov 10 08:44:21 2006 From: ASenn <@t> mercy.pmhs.org (Senn, Amy) Date: Fri Nov 10 08:45:18 2006 Subject: [Histonet] Autopsy videos Message-ID: <81C95EFFB67F284B9FC080B91954F81DD61A6D@pmhs2kxch03> Peggy, I agree..there are some sick people out there---I've looked for sites too, and geez...the stuff I found :( I'd also be interested in training videos for dieners--as I've been hired to assist ours and become a diener myself. Thanks all!! Amy Message: 13 Date: Thu, 9 Nov 2006 05:39:29 -0500 From: "Lee & Peggy Wenk" Subject: [Histonet] Autopsy training videos To: Message-ID: <002901c703eb$5670cf20$baec2d4b@HPPav2> Content-Type: text/plain; charset="us-ascii" Does anyone know where there are training videos/CD/DVD for how to do an autopsy. Looking for something to supplement teaching first year residents and new dieners (people who assist with the autopsies). Looking on the internet gets me into sites I don't want to get into. There are some sick people out there. Thanks. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 From lchung <@t> ppmh.org Fri Nov 10 08:46:05 2006 From: lchung <@t> ppmh.org (Chung, Luong) Date: Fri Nov 10 08:46:32 2006 Subject: [Histonet] Friday Fun Message-ID: <86691924ECCDBE4F82CCAB5534246071019428@exchange2.phoebe.com> Bubbles make slide look pretty and fun to look at. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Weems, Joyce Sent: Friday, November 10, 2006 9:38 AM To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Friday Fun "There is really no need for a tech to work Saturdays any more. We'll just have someone on call." -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Breeden, Sara Sent: Friday, November 10, 2006 8:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Friday Fun Okay, here's the deal. Tell me, concisely, what would be the most unlikely thing a histologist would hear from a pathologist? For example, "No, please! Don't recut that broken slide - I can see just fine through the cellophane tape". Send your innovative, imaginative, and funny responses directly to me at sbreeden@nmda.nmsu.edu. This could lead to a Very Funny Friday! "Start your engines!!" Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. ----------------------------------------- Disclaimer: The HIPAA Final Privacy Rule requires covered entities to safeguard certain Protected Health Information (PHI) related to a person's healthcare. Information being faxed to you may include PHI after appropriate authorization from the patient or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain PHI in a safe and secure manner. You may not re-disclose without additional patient consent or as required by law. Unauthorized re-disclosure or failure to safeguard PHI could subject you to penalties described in federal (HIPAA) and state law. If you the reader of this message are not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, please notify us immediately and destroy the related message. From stamptrain <@t> yahoo.com Fri Nov 10 08:47:17 2006 From: stamptrain <@t> yahoo.com (Roger Moretz) Date: Fri Nov 10 08:47:23 2006 Subject: [Histonet] Re: Cut resistant gloves In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBFAFC92C@wahtntex2.waht.swest.nhs.uk> Message-ID: <634538.13265.qm@web50308.mail.yahoo.com> No. Harleys are the real bikes. All the rest are just toys (unless you mean Indians or BMWs) Roger Moretz --- Kemlo Rogerson wrote: > Harley Davidson? Oh I see those big old bikes that > are VERY VERY SLOW > and don't go round corners. No I meant real bikes, > Yamaha, Suzuki, > Honda, good british bikes!!!! > > > Kemlo Rogerson > Pathology Manager > Ext 3311 > DD 01934 647057 > Mob 07749 754194 > Pager 07659 597107 > E-Mail: kemlo.rogerson@nhs.net > Habits of thinking need not be forever. One of the > most significant > findings in psychology in the last twenty years is > that individuals can > choose the way they think. --Martin Seligman > > > > This e-mail is confidential and privileged. If you > are not the intended > recipient please accept my apologies; please do not > disclose, copy or > distribute information in this e-mail or take any > action in reliance on > its contents: to do so is strictly prohibited and > may be unlawful. > Please inform me that this message has gone astray > before deleting it. > Thank you for your co-operation > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ____________________________________________________________________________________ Want to start your own business? Learn how on Yahoo! Small Business. http://smallbusiness.yahoo.com/r-index From Lynne.Bell <@t> hitchcock.org Fri Nov 10 08:53:15 2006 From: Lynne.Bell <@t> hitchcock.org (Bell, Lynne) Date: Fri Nov 10 08:53:30 2006 Subject: [Histonet] Friday Fun In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B0DB329@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: "Don't worry about it - I'll clean the grossing area myself." Lynne Bell, HT(ASCP) Department of Pathology Central VT Medical Center P. O. Box 547 Barre, VT 05641 802-371-4923 From ree3 <@t> leicester.ac.uk Fri Nov 10 08:59:37 2006 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri Nov 10 08:59:44 2006 Subject: [Histonet] Special Stain "Storage" In-Reply-To: Message-ID: Or analysandum!. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: 09 November 2006 18:11 To: Histonet (E-mail) Subject: RE: [Histonet] Special Stain "Storage" Just remember, you can't spell "analysis" without "anal" !!!! Terri -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Philip Oshel Sent: Thursday, November 09, 2006 12:43 PM To: Histonet@Pathology.swmed.edu Subject: RE: [Histonet] Special Stain "Storage" Hm. Are histotech anal-retentive? Is anal-retentive spelled with a hyphen or not? >Terri & Shirley! > >I've been away from histonet for a while... i sort of "retired" a few >years ago to be a stay-at-home mom. WOW! histology is so much easier >than that job!!! :) > >i'm back a few days a week, now... and i guess i didn't realize how >much i missed it. the stay-at-home gig made me a little crazy(er). > >i truly believe my histo experience sort of bled into my stay-at-home >life... and made it much more organized. i have all sorts of >histo-inspired organization in my house. (my kids have boxes that would >rival your best special stain storage) and anyone old enough has a >daily maintenance log. :) people that have seen it... think it's >bizarre but my house is always about 15 minutes from being >"mother-in-law-is-making-a-surprise-visit" ready!) > >one wonders... are most histotechs obsessive-compulsive as a RESULT of >histo... OR did the OCD DRAW you TO histo. it's the age-old >question... chicken or egg.... histo or OCD. > >:) -- Philip Oshel Microscopy Facility Supervisor Biology Department Central Michigan University 024C Brooks Hall Mt. Pleasant, MI 48859 voice: (989) 774-3576 dept. fax: (989) 774-3462 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Fri Nov 10 09:00:40 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Nov 10 09:00:50 2006 Subject: [Histonet] Friday Fun Message-ID: <63984BC3A63FF542AF6EF0A237F38F4D7E4C98@LIL.xRothGen.nhs.uk> Huh? I do that. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Bell, Lynne [mailto:Lynne.Bell@hitchcock.org] Sent: 10 November 2006 14:53 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Friday Fun "Don't worry about it - I'll clean the grossing area myself." Lynne Bell, HT(ASCP) Department of Pathology Central VT Medical Center P. O. Box 547 Barre, VT 05641 802-371-4923 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From froyer <@t> bitstream.net Fri Nov 10 09:07:10 2006 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Nov 10 09:07:52 2006 Subject: [Histonet] Friday Fun In-Reply-To: <86691924ECCDBE4F82CCAB5534246071019428@exchange2.phoebe.com> Message-ID: <003001c704d9$e8359af0$7701a80a@Ford> "Here, let me close that lid to the cryostat. You know... if you leave it open all the time, it really frosts up!" Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. http://www.minnesotamedical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chung, Luong Sent: Friday, November 10, 2006 8:46 AM To: Weems, Joyce; Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Friday Fun Bubbles make slide look pretty and fun to look at. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Weems, Joyce Sent: Friday, November 10, 2006 9:38 AM To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Friday Fun "There is really no need for a tech to work Saturdays any more. We'll just have someone on call." -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Breeden, Sara Sent: Friday, November 10, 2006 8:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Friday Fun Okay, here's the deal. Tell me, concisely, what would be the most unlikely thing a histologist would hear from a pathologist? For example, "No, please! Don't recut that broken slide - I can see just fine through the cellophane tape". Send your innovative, imaginative, and funny responses directly to me at sbreeden@nmda.nmsu.edu. This could lead to a Very Funny Friday! "Start your engines!!" Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. ----------------------------------------- Disclaimer: The HIPAA Final Privacy Rule requires covered entities to safeguard certain Protected Health Information (PHI) related to a person's healthcare. Information being faxed to you may include PHI after appropriate authorization from the patient or under circumstances that do not require patient authorization. You, the recipient, are obligated to maintain PHI in a safe and secure manner. You may not re-disclose without additional patient consent or as required by law. Unauthorized re-disclosure or failure to safeguard PHI could subject you to penalties described in federal (HIPAA) and state law. If you the reader of this message are not the intended recipient, or the employee or agent responsible to deliver it to the intended recipient, please notify us immediately and destroy the related message. From BMolinari <@t> heart.thi.tmc.edu Fri Nov 10 09:15:53 2006 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Nov 10 09:16:01 2006 Subject: [Histonet] Friday Fun In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEF14F@sjhaexc02.sjha.org> Message-ID: "Would you please show me how you would like breast tissue grossed in..to make your life easier? Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, November 10, 2006 8:38 AM To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Friday Fun "There is really no need for a tech to work Saturdays any more. We'll just have someone on call." -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Breeden, Sara Sent: Friday, November 10, 2006 8:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Friday Fun Okay, here's the deal. Tell me, concisely, what would be the most unlikely thing a histologist would hear from a pathologist? For example, "No, please! Don't recut that broken slide - I can see just fine through the cellophane tape". Send your innovative, imaginative, and funny responses directly to me at sbreeden@nmda.nmsu.edu. This could lead to a Very Funny Friday! "Start your engines!!" Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From BMolinari <@t> heart.thi.tmc.edu Fri Nov 10 09:17:29 2006 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Nov 10 09:17:35 2006 Subject: [Histonet] Re: Cut resistant gloves In-Reply-To: <634538.13265.qm@web50308.mail.yahoo.com> Message-ID: BMW's? You are kidding right????? Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roger Moretz Sent: Friday, November 10, 2006 8:47 AM To: Kemlo Rogerson; RSRICHMOND@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Cut resistant gloves No. Harleys are the real bikes. All the rest are just toys (unless you mean Indians or BMWs) Roger Moretz --- Kemlo Rogerson wrote: > Harley Davidson? Oh I see those big old bikes that > are VERY VERY SLOW > and don't go round corners. No I meant real bikes, > Yamaha, Suzuki, > Honda, good british bikes!!!! > > > Kemlo Rogerson > Pathology Manager > Ext 3311 > DD 01934 647057 > Mob 07749 754194 > Pager 07659 597107 > E-Mail: kemlo.rogerson@nhs.net > Habits of thinking need not be forever. One of the > most significant > findings in psychology in the last twenty years is > that individuals can > choose the way they think. --Martin Seligman > > > > This e-mail is confidential and privileged. If you > are not the intended > recipient please accept my apologies; please do not > disclose, copy or > distribute information in this e-mail or take any > action in reliance on > its contents: to do so is strictly prohibited and > may be unlawful. > Please inform me that this message has gone astray > before deleting it. > Thank you for your co-operation > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ________________________________________________________________________ ____________ Want to start your own business? Learn how on Yahoo! Small Business. http://smallbusiness.yahoo.com/r-index _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmarcum <@t> vet.upenn.edu Fri Nov 10 09:19:06 2006 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Fri Nov 10 09:19:14 2006 Subject: [Histonet] Friday Fun In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B0DB329@nmdamailsvr.nmda.ad .nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B0DB329@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <6.2.5.6.2.20061110101322.01c2a7b8@vet.upenn.edu> At 08:05 AM 11/10/2006, Breeden, Sara wrote: >Okay, here's the deal. Tell me, concisely, what would be the most >unlikely thing a histologist would hear from a pathologist? For >example, "No, please! Don't recut that broken slide - I can see just >fine through the cellophane tape". Send your innovative, imaginative, >and funny responses directly to me at sbreeden@nmda.nmsu.edu. This >could lead to a Very Funny Friday! "Start your engines!!" > > > >Sally Breeden, HT(ASCP) > >NM Dept. of Agriculture > >Veterinary Diagnostic Services > >PO Box 700 > >Albuquerque, NM 87106 > >505-841-2576 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet " I will always gross at 3mm or less just to help you out from now on, especially fatty specimens. I will even teach the residents to gross smaller!" Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From portera <@t> msu.edu Fri Nov 10 09:24:11 2006 From: portera <@t> msu.edu (Amy Porter) Date: Fri Nov 10 09:22:20 2006 Subject: [Histonet] Help with immunohistochemistry in human embryos References: <004d01c70416$1b5b7a40$5063e7c9@ARIEL> Message-ID: <000901c704dc$467e5870$8e7a0923@histolab> You might want to try using a Heat Epitope Induced Retrieval protocol. Most of Santa Cruz's antibodies will work with heat retrieval. Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 355-6475 ext 1480 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu ----- Original Message ----- From: "Hernan Aldana" To: Sent: Thursday, November 09, 2006 10:45 AM Subject: [Histonet] Help with immunohistochemistry in human embryos Dear Histoneters I need some advices. I'm working with human embryos between 7 and 14 weeks. we used Sonic Hedgehog Shh (C-18) and sc-1195 antibodies of Santa Cruz Biotechnology in paraffin sections of embryos fixed in paraformadehyde. I don't have good results. Could anybody help me with some advice, concerning to every step of my technique? Thanks in advance Dr. Hern?n Javier Aldana Marcos Prof. Titular Reg. Histolog?a, Embriolog?a y Biolog?a Celular Facultad de Medicina Universidad de Mor?n Argentina email altenrativo: hernanjavier@yahoo.com http://www.ht.org.ar/educacion.htm -----Mensaje original----- De: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] En nombre de histonet-request@lists.utsouthwestern.edu Enviado el: Domingo, 01 de Octubre de 2006 02:04 p.m. Para: histonet@lists.utsouthwestern.edu Asunto: Histonet Digest, Vol 35, Issue 1 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Job Opening (Luck, Greg D.) 2. RE: Problem of Double immunoflouresence staining .. (Melissa Gonzalez) 3. The procedure that substitues xylene with mineral oil. (PhiHo Hoang) 4. Re: The procedure that substitues xylene with mineral oil. (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Sat, 30 Sep 2006 12:01:02 -0700 From: "Luck, Greg D." Subject: [Histonet] Job Opening To: Cc: "Ringwood, James F." , "Wakabayashi, Marlene T." , "Browning, Rachel M." Message-ID: <6BB8BC4519AAB844B174FC739A679BBC50900C@IRMEXCH01.irm.inhs.org> Content-Type: text/plain; charset="us-ascii" Hello All, Deaconess is a 300 bed acute care community medical center. We have a full time, M-F, day time position open. Must be HT(ASCP) certified or eligible. Duties include all routine histology functions in a clinical setting (i.e. embedding , cutting, special stains and frozen sections). IHC experience preferred. Due to a recent market adjustment by our organization the salary range for this position has just increased 25-33% depending on years of experience. This is a golden opportunity for someone to improve on their current situation or to begin anew. Anyone new to this field is encouraged to apply as well, for the door is wide open. You can apply on-line by going to our corporate website www.empirehealth.org. The position is at Deaconess Medical Center in Spokane, WA www.deaconess-spokane.org. Please contact me directly for more details. Following is an additional web link to provide you with more info on Spokane and the greater Inland Northwest region www.visitspokane.com. ("Near Perfect-Near Nature"). Thank you and best wishes, Greg Greg Luck, BS, HT(ASCP) Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 Phone 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconess-spokane.org ------------------------------ Message: 2 Date: Sun, 1 Oct 2006 08:40:44 -0700 From: "Melissa Gonzalez" Subject: [Histonet] RE: Problem of Double immunoflouresence staining .. To: Message-ID: Content-Type: text/plain; charset="us-ascii" Sohail, First, Perhaps you should try incubating each primary with respective secondary only, to determine how they look separately, and also make sure each is working on its own. For example, you may not have your aSMA dilution correct. Also, do you have a positive control for TRITC to make sure you can detect any TRITC signal adequately? Once you have appropriate signal from both, you can then make your cocktails. Also, in the future, I would recommend switching to Alexa Fluors from Molecular Probes, they really are great. In your instance you would use AF488 (FITC) and AF 546 or 555 (TRITC). But that is just my 2 cents. Good luck, Melissa Message: 4 Date: Fri, 29 Sep 2006 13:06:27 -0700 (PDT) From: sohail ejaz Subject: [Histonet] Problem of Double immunoflouresence staining of retinal vasculature To: histonet@lists.utsouthwestern.edu Message-ID: <20060929200627.949.qmail@web39506.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello everybody I have a big problem of staining retina vasculature using a cocktail of two different antibodies. The problem is that i can only see staining with FITC but not with TRITC, hence i cant make a merger of both FITC and TRITC. Cocktail of primary antibodies include (1) Monoclonal Rabbit Anti human vWF (2) Monoclonal mouse Anti alpha SMA and cocktail of secondary antibodies include (1) Anti rabbit FITC (2) Anti mouse TRITC Please let me know your commentc to slove this issue. Dr.Sohail ------------------------------ Message: 3 Date: Sun, 1 Oct 2006 11:47:56 -0400 From: "PhiHo Hoang" Subject: [Histonet] The procedure that substitues xylene with mineral oil. To: Message-ID: <005b01c6e570$f725b820$f300a8c0@ttth> Content-Type: text/plain; charset="iso-8859-1" Greetings, I am a new subscriber to this list and I am reading the list archive. I came across a posting from Reni J. responding to a request from Sharon: Sharon: I am sending privately a procedure I have that substitutes xylene with mineral oil. It is 100 times less toxic than xylene and 3 times cheaper. Reni J. Sharon Allen exchange.hsc.mb.ca> wrote: Does anyone use xylol substitutes, if so how good are they? We do CJD testing & "the powers that be" don't like to pay for getting rid of contaminated xylol. I don't want to have to start testing different substitutes, so any help would be greatly appreciated. Thanks Sharon sallen <@t> hsc.mb.ca I am interested in this procedure. It is very much appreciated if I can get a copy of that procedure. Best regards, PhiHo ------------------------------ Message: 4 Date: Sun, 1 Oct 2006 09:55:53 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] The procedure that substitues xylene with mineral oil. To: PhiHo Hoang , histonet@lists.utsouthwestern.edu Message-ID: <20061001165553.32345.qmail@web61219.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 PhiHo: I am attaching the procedure under separate cover. Reni J. PhiHo Hoang wrote: Greetings, I am a new subscriber to this list and I am reading the list archive. I came across a posting from Reni J. responding to a request from Sharon: Sharon: I am sending privately a procedure I have that substitutes xylene with mineral oil. It is 100 times less toxic than xylene and 3 times cheaper. Reni J. Sharon Allen exchange.hsc.mb.ca> wrote: Does anyone use xylol substitutes, if so how good are they? We do CJD testing & "the powers that be" don't like to pay for getting rid of contaminated xylol. I don't want to have to start testing different substitutes, so any help would be greatly appreciated. Thanks Sharon sallen <@t> hsc.mb.ca I am interested in this procedure. It is very much appreciated if I can get a copy of that procedure. Best regards, PhiHo _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1"/min. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 35, Issue 1 *************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Fri Nov 10 09:25:09 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Nov 10 09:29:26 2006 Subject: [Histonet] Re: Cut resistant gloves Message-ID: <63984BC3A63FF542AF6EF0A237F38F4D7E4C9A@LIL.xRothGen.nhs.uk> Agree (for a change). Harley's are big, ugly, sound like tractors, and invariably ridden by apes. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk] Sent: 10 November 2006 10:02 To: RSRICHMOND@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Cut resistant gloves Harley Davidson? Oh I see those big old bikes that are VERY VERY SLOW and don't go round corners. No I meant real bikes, Yamaha, Suzuki, Honda, good british bikes!!!! Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Habits of thinking need not be forever. One of the most significant findings in psychology in the last twenty years is that individuals can choose the way they think. --Martin Seligman This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bernaweston <@t> hotmail.com Fri Nov 10 09:29:38 2006 From: bernaweston <@t> hotmail.com (Bernadette Weston) Date: Fri Nov 10 09:29:52 2006 Subject: [Histonet] Mohs surgeries Message-ID: Does anyone out there do Mohs surgeries in their hospital as a separate entity not part of the Pathology department? Bernadette Weston HT Barberton Citizens Hospital Barberton, OH _________________________________________________________________ All-in-one security and maintenance for your PC. Get a free 90-day trial! http://clk.atdmt.com/MSN/go/msnnkwlo0050000002msn/direct/01/?href=http://www.windowsonecare.com/?sc_cid=msn_hotmail From debbiekeith <@t> cox.net Fri Nov 10 09:31:43 2006 From: debbiekeith <@t> cox.net (Debbie Keith) Date: Fri Nov 10 09:31:51 2006 Subject: [Histonet] Re: Cut resistant gloves In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBFAFC92C@wahtntex2.waht.swes t.nhs.uk> Message-ID: <5.2.0.9.0.20061110083002.00c14238@pop.central.cox.net> At 10:02 AM 11/10/2006 +0000, you wrote: >Harley Davidson? Oh I see those big old bikes that are VERY VERY SLOW >and don't go round corners. No I meant real bikes, Yamaha, Suzuki, >Honda, good british bikes!!!! bah! i like to SEE the harley TRY to corner like a real bike...(the sparks from the pegs and pipes amuse me). ;) i ride a triumph.... THAT is a good british bike! deb -- No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.1.409 / Virus Database: 268.14.0/525 - Release Date: 11/9/2006 From froyer <@t> bitstream.net Fri Nov 10 09:35:46 2006 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Nov 10 09:36:23 2006 Subject: [Histonet] Re: Cut resistant gloves In-Reply-To: Message-ID: <003b01c704dd$e4851940$7701a80a@Ford> Next to Harley, one of the finest bikes ever made. (You did know that BMW made motorcycles didn't you?) ;-) Ford -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Friday, November 10, 2006 9:17 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Cut resistant gloves BMW's? You are kidding right????? Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) From Terry.Marshall <@t> rothgen.nhs.uk Fri Nov 10 09:40:24 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Nov 10 09:40:33 2006 Subject: [Histonet] Friday Fun Message-ID: <63984BC3A63FF542AF6EF0A237F38F4D7E4C9B@LIL.xRothGen.nhs.uk> >From my colleagues: "The slides were poor today". Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Breeden, Sara [mailto:sbreeden@nmda.nmsu.edu] Sent: 10 November 2006 13:06 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Friday Fun Okay, here's the deal. Tell me, concisely, what would be the most unlikely thing a histologist would hear from a pathologist? For example, "No, please! Don't recut that broken slide - I can see just fine through the cellophane tape". Send your innovative, imaginative, and funny responses directly to me at sbreeden@nmda.nmsu.edu. This could lead to a Very Funny Friday! "Start your engines!!" Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sheila_adey <@t> hotmail.com Fri Nov 10 09:45:49 2006 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Fri Nov 10 09:46:02 2006 Subject: [Histonet] Friday Fun In-Reply-To: Message-ID: "I'm sorry, I didn't realize you can't embed a 1cm thick piece of tissue" Sheila Adey HT MLT Port Huron Hospital Michigan >From: "Molinari, Betsy" >To: >Subject: RE: [Histonet] Friday Fun >Date: Fri, 10 Nov 2006 09:15:53 -0600 > >"Would you please show me how you would like breast tissue grossed >in..to make your life easier? > >Betsy Molinari HT (ASCP) >Texas Heart Institute >Cardiovascular Pathology >6770 Bertner Ave. >Houston,TX 77030 >832-355-6524 >832-355-6812 (fax) > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, >Joyce >Sent: Friday, November 10, 2006 8:38 AM >To: Breeden, Sara; histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] Friday Fun > >"There is really no need for a tech to work Saturdays any more. We'll >just have someone on call." > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Breeden, >Sara >Sent: Friday, November 10, 2006 8:06 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Friday Fun > > >Okay, here's the deal. Tell me, concisely, what would be the most >unlikely thing a histologist would hear from a pathologist? For >example, "No, please! Don't recut that broken slide - I can see just >fine through the cellophane tape". Send your innovative, imaginative, >and funny responses directly to me at sbreeden@nmda.nmsu.edu. This >could lead to a Very Funny Friday! "Start your engines!!" > > > >Sally Breeden, HT(ASCP) > >NM Dept. of Agriculture > >Veterinary Diagnostic Services > >PO Box 700 > >Albuquerque, NM 87106 > >505-841-2576 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >Confidentiality Notice ** The information contained in this message may >be privileged and is confidential information intended for the use of >the addressee listed above. If you are neither the intended recipient >nor the employee or agent responsible for delivering this message to the >intended recipient, you are hereby notified that any disclosure, >copying, distribution or the taking of any action in reliance on the >contents of this information is strictly prohibited. If you have >received this communication in error, please notify us immediately by >replying to the message and deleting it from your computer. Thank you. >Saint Joseph's Health System, Inc. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Not only does Windows Live™ OneCare™ provide all-in-one PC care to keep your computer protected and well-maintained, but it also makes creating backup files a breeze. Try it today! http://ideas.live.com/programpage.aspx?versionid=b2456790-90e6-4d28-9219-5d7207d94d45&mkt=en-ca From Jackie.O'Connor <@t> abbott.com Fri Nov 10 09:49:23 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Nov 10 09:50:00 2006 Subject: [Histonet] Something new In-Reply-To: <003001c704d9$e8359af0$7701a80a@Ford> Message-ID: Can someone advise me the best way to preserve (Liguid nitrogen, OCT, fixed?) and visualize tissues from animals which have been administered (in vivo) a fluorescent probe? Thanks, Jackie O' on a crummy Friday morning fighting fires again as usual. From godsgalnow <@t> aol.com Fri Nov 10 09:51:40 2006 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Fri Nov 10 09:51:56 2006 Subject: [Histonet] specimen rejection Message-ID: <8C8D2F73F1AC313-ED0-BDAA@WEBMAIL-MA14.sysops.aol.com> Ayone have a specimen rejection policy they would be willing to share? Thanks is advance. Roxanne ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From bwhitaker <@t> brownpathology.com Fri Nov 10 09:55:53 2006 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Fri Nov 10 09:52:22 2006 Subject: [Histonet] Friday Fun In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B0DB329@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: <002001c704e0$b3c2ec30$3601a8c0@brownpathology.net> How about: "You work so hard, and I appreciate it so much! Why don't you sleep in on Monday? I'll cut my own slides." Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, November 10, 2006 7:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Friday Fun Okay, here's the deal. Tell me, concisely, what would be the most unlikely thing a histologist would hear from a pathologist? For example, "No, please! Don't recut that broken slide - I can see just fine through the cellophane tape". Send your innovative, imaginative, and funny responses directly to me at sbreeden@nmda.nmsu.edu. This could lead to a Very Funny Friday! "Start your engines!!" Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Fri Nov 10 10:03:45 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Nov 10 10:04:17 2006 Subject: [Histonet] Re: How efficient is formalin in killing HIV and Hepatitis? Message-ID: <3bb.6cc8b8b3.3285fce1@aol.com> Teri Johnson, HT(ASCP)QIHC at Stowers Institute for Medical Research in Kansas City, Missouri asks: >>Does anybody have published guidelines on how much time it takes for formalin (10% NBF) to effectively render HIV and Hepatitis non-infective?<< Obviously it would depend on the thickness of the specimen. HIV would be inactivated very rapidly. The hepatitis B virus is hardier, but it would not withstand more than very brief fixation. I think less is known about the hepatitis C virus, but it's more fragile than B. Certainly any tissue processing cycle for a properly cut specimen would suffice. Remember that alcohol is also a very potent viricidal agent. Bob Richmond Samurai Pathologist Knoxville TN From ree3 <@t> leicester.ac.uk Fri Nov 10 10:04:44 2006 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri Nov 10 10:04:54 2006 Subject: [Histonet] Friday Fun In-Reply-To: <6.2.5.6.2.20061110101322.01c2a7b8@vet.upenn.edu> Message-ID: I did immunostaining once, worked for me every time!. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: 10 November 2006 15:19 To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Friday Fun At 08:05 AM 11/10/2006, Breeden, Sara wrote: >Okay, here's the deal. Tell me, concisely, what would be the most >unlikely thing a histologist would hear from a pathologist? For >example, "No, please! Don't recut that broken slide - I can see just >fine through the cellophane tape". Send your innovative, imaginative, >and funny responses directly to me at sbreeden@nmda.nmsu.edu. This >could lead to a Very Funny Friday! "Start your engines!!" > > > >Sally Breeden, HT(ASCP) > >NM Dept. of Agriculture > >Veterinary Diagnostic Services > >PO Box 700 > >Albuquerque, NM 87106 > >505-841-2576 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet " I will always gross at 3mm or less just to help you out from now on, especially fatty specimens. I will even teach the residents to gross smaller!" Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MMargiotta <@t> bmhmc.org Fri Nov 10 10:08:11 2006 From: MMargiotta <@t> bmhmc.org (Margiotta, Michele) Date: Fri Nov 10 10:08:25 2006 Subject: [Histonet] paraffin time Message-ID: <922CE5B88F398948B4076A9A4340E7AF036AF5E4@bmh_exchange.bmhmc.org> Hi All, Wondering if anyone has an idea how long small bxs. can remain in the last wax station after the processing is completed. We had a problem with that today and a lot of our bxs look "cooked". Thanks! Michele This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. From llewllew <@t> shaw.ca Fri Nov 10 10:14:17 2006 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Fri Nov 10 10:15:05 2006 Subject: [Histonet] Friday Fun Message-ID: <005401c704e3$45bdc360$130e4246@yourlk4rlmsu> "Please recut the slide thicker, It's too thin" > ----- Original Message ----- > From: "Breeden, Sara" > To: > Sent: Friday, November 10, 2006 5:05 AM > Subject: [Histonet] Friday Fun > > > Okay, here's the deal. Tell me, concisely, what would be the most > unlikely thing a histologist would hear from a pathologist? For > example, "No, please! Don't recut that broken slide - I can see just > fine through the cellophane tape". Send your innovative, imaginative, > and funny responses directly to me at sbreeden@nmda.nmsu.edu. This > could lead to a Very Funny Friday! "Start your engines!!" > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From PMonfils <@t> Lifespan.org Fri Nov 10 10:15:40 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Nov 10 10:15:57 2006 Subject: [Histonet] Friday Fun Message-ID: <4EBFF65383B74D49995298C4976D1D5E273BAC@LSRIEXCH1.lsmaster.lifespan.org> I don't need to see the slide, I'll take your word for it. I know it's STAT, but you still need your lunch. From RSRICHMOND <@t> aol.com Fri Nov 10 10:15:58 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Nov 10 10:16:14 2006 Subject: [Histonet] Re: Cut resistant gloves Message-ID: I went to a Harley Davidson shop for no better reason than that I saw it on the side of the road. I don't know of any published studies, but I think that all makes of donorcycles are equally efficient in producing brain-dead organ donors. Bob Richmond From Terry.Marshall <@t> rothgen.nhs.uk Fri Nov 10 10:22:20 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Nov 10 10:22:35 2006 Subject: [Histonet] paraffin time Message-ID: <63984BC3A63FF542AF6EF0A237F38F4D7E4C9C@LIL.xRothGen.nhs.uk> This "cooked" look crops up with monotonous regularity, but I have yet to see it defined in a comprehensible fashion, or seen a photograph upon which we can all agree is a "cooked look". Since heat is a satisfactory form of fixation, how can something look cooked? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Margiotta, Michele [mailto:MMargiotta@bmhmc.org] Sent: 10 November 2006 16:08 To: histonet@pathology.swmed.edu Subject: [Histonet] paraffin time Hi All, Wondering if anyone has an idea how long small bxs. can remain in the last wax station after the processing is completed. We had a problem with that today and a lot of our bxs look "cooked". Thanks! Michele This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Fri Nov 10 10:23:24 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Nov 10 10:23:31 2006 Subject: [Histonet] Friday Fun Message-ID: <63984BC3A63FF542AF6EF0A237F38F4D7E4C9D@LIL.xRothGen.nhs.uk> I say that. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Bryan Llewellyn [mailto:llewllew@shaw.ca] Sent: 10 November 2006 16:14 To: Histonet Subject: Re: [Histonet] Friday Fun "Please recut the slide thicker, It's too thin" > ----- Original Message ----- > From: "Breeden, Sara" > To: > Sent: Friday, November 10, 2006 5:05 AM > Subject: [Histonet] Friday Fun > > > Okay, here's the deal. Tell me, concisely, what would be the most > unlikely thing a histologist would hear from a pathologist? For > example, "No, please! Don't recut that broken slide - I can see just > fine through the cellophane tape". Send your innovative, imaginative, > and funny responses directly to me at sbreeden@nmda.nmsu.edu. This > could lead to a Very Funny Friday! "Start your engines!!" > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Fri Nov 10 10:25:02 2006 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Nov 10 10:25:32 2006 Subject: [Histonet] Friday Fun Message-ID: <6CBA6DC98A079D408C87250591D9DFB802684BBF@bruexchange.digestivespecialists.com> Ok Sally! You're going to have to compile all of these! Linda :) Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937)293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Friday, November 10, 2006 8:06 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Friday Fun Okay, here's the deal. Tell me, concisely, what would be the most unlikely thing a histologist would hear from a pathologist? For example, "No, please! Don't recut that broken slide - I can see just fine through the cellophane tape". Send your innovative, imaginative, and funny responses directly to me at sbreeden@nmda.nmsu.edu. This could lead to a Very Funny Friday! "Start your engines!!" Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Fri Nov 10 10:24:39 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Nov 10 10:29:43 2006 Subject: [Histonet] Friday Fun Message-ID: <63984BC3A63FF542AF6EF0A237F38F4D7E4C9E@LIL.xRothGen.nhs.uk> I say that. (e.g. Crystals in joint fluid. Acellular specimen.) Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Monfils, Paul [mailto:PMonfils@Lifespan.org] Sent: 10 November 2006 16:16 To: 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Friday Fun I don't need to see the slide, I'll take your word for it. I know it's STAT, but you still need your lunch. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Nov 10 10:33:16 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Nov 10 10:33:32 2006 Subject: [Histonet] paraffin time In-Reply-To: <922CE5B88F398948B4076A9A4340E7AF036AF5E4@bmh_exchange.bmhmc.org> Message-ID: <20061110163316.56787.qmail@web61214.mail.yahoo.com> The shortest time the better = the time prescribed in your proven protocol. Ren? J. "Margiotta, Michele" wrote: Hi All, Wondering if anyone has an idea how long small bxs. can remain in the last wax station after the processing is completed. We had a problem with that today and a lot of our bxs look "cooked". Thanks! Michele This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Check out the all-new Yahoo! Mail beta - Fire up a more powerful email and get things done faster. From ree3 <@t> leicester.ac.uk Fri Nov 10 10:35:58 2006 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri Nov 10 10:36:14 2006 Subject: [Histonet] Friday Fun In-Reply-To: Message-ID: and heard in the good old days...."Can you hold the cigarette for me I need two hands for this" -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: 10 November 2006 15:19 To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Friday Fun At 08:05 AM 11/10/2006, Breeden, Sara wrote: >Okay, here's the deal. Tell me, concisely, what would be the most >unlikely thing a histologist would hear from a pathologist? For >example, "No, please! Don't recut that broken slide - I can see just >fine through the cellophane tape". Send your innovative, imaginative, >and funny responses directly to me at sbreeden@nmda.nmsu.edu. This >could lead to a Very Funny Friday! "Start your engines!!" > > > >Sally Breeden, HT(ASCP) > >NM Dept. of Agriculture > >Veterinary Diagnostic Services > >PO Box 700 > >Albuquerque, NM 87106 > >505-841-2576 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet " I will always gross at 3mm or less just to help you out from now on, especially fatty specimens. I will even teach the residents to gross smaller!" Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Fri Nov 10 10:38:20 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Nov 10 10:38:47 2006 Subject: [Histonet] Friday Fun Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEF153@sjhaexc02.sjha.org> as ashes were flicked into the trash can full of paraffin scraps and xylene soaked gauze..... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Edwards, R.E. Sent: Friday, November 10, 2006 11:36 AM To: Edwards, R.E.; Pamela Marcum; Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Friday Fun and heard in the good old days...."Can you hold the cigarette for me I need two hands for this" -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: 10 November 2006 15:19 To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Friday Fun At 08:05 AM 11/10/2006, Breeden, Sara wrote: >Okay, here's the deal. Tell me, concisely, what would be the most >unlikely thing a histologist would hear from a pathologist? For >example, "No, please! Don't recut that broken slide - I can see just >fine through the cellophane tape". Send your innovative, imaginative, >and funny responses directly to me at sbreeden@nmda.nmsu.edu. This >could lead to a Very Funny Friday! "Start your engines!!" > > > >Sally Breeden, HT(ASCP) > >NM Dept. of Agriculture > >Veterinary Diagnostic Services > >PO Box 700 > >Albuquerque, NM 87106 > >505-841-2576 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet " I will always gross at 3mm or less just to help you out from now on, especially fatty specimens. I will even teach the residents to gross smaller!" Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From pjfnefro <@t> duke.edu Fri Nov 10 10:39:00 2006 From: pjfnefro <@t> duke.edu (Pat Flannery) Date: Fri Nov 10 10:39:09 2006 Subject: [Histonet] Re: Cut resistant gloves In-Reply-To: References: Message-ID: <05F5B44B-B8EF-4252-917B-290493088E3D@duke.edu> OUCH! I suppose having "organ donor" on my motorcycle license is redundant, then. :-) I used to think that crotch-rockets were more efficient in producing donor organs, but, in my experience, the speed with which they're usually employed (by the young folks who usually ride them) actually leaves very little to donate. I now believe that cruisers and touring bikes (and the older, wiser, folks who usually ride them) produce more intact (if slightly used) donor organs. Thanks for reminding me, though. :-) -Patrick J. (Pat) Flannery Division of Nephrology (that's kidneys to you) Box 3014 (that's NOT "PO" just "Box") Duke University Medical Center (although I went to UNC-CH) Durham, NC 27710 E-mail: pjfnefro@duke.edu (preferred) FLANN002@MC.DUKE.EDU (also works) Voice: (919)660-6863 Fax: (919)684-2929 Cell: (919)606-0163 On Nov 10, 2006, at 11:15 AM, RSRICHMOND@aol.com wrote: > I went to a Harley Davidson shop for no better reason than that I > saw it on > the side of the road. > > I don't know of any published studies, but I think that all makes of > donorcycles are equally efficient in producing brain-dead organ > donors. > > Bob Richmond > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tmhpath <@t> amigo.net Fri Nov 10 10:49:07 2006 From: tmhpath <@t> amigo.net (Michelle D. Moore) Date: Fri Nov 10 10:49:17 2006 Subject: [Histonet] Fw: Funny Friday Message-ID: <004301c704e8$24295200$ef00a8c0@tmhpath1> This may not be unlikely to hear but we thought it was kind of funny at the time Our pathologist left us a note that asked us to do a "Helicopter stain" on a gastric specimen, so my tech at the time asked him if he wanted us to do that at the airport. Michelle D. Moore TMH Craig, CO From lblazek <@t> digestivespecialists.com Fri Nov 10 10:51:25 2006 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Nov 10 10:51:06 2006 Subject: [Histonet] Friday Fun Message-ID: <6CBA6DC98A079D408C87250591D9DFB802684BC0@bruexchange.digestivespecialists.com> And the coffee cup was on top of the microtome Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, November 10, 2006 11:38 AM To: Edwards, R.E.; Pamela Marcum; Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Friday Fun as ashes were flicked into the trash can full of paraffin scraps and xylene soaked gauze..... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Edwards, R.E. Sent: Friday, November 10, 2006 11:36 AM To: Edwards, R.E.; Pamela Marcum; Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Friday Fun and heard in the good old days...."Can you hold the cigarette for me I need two hands for this" -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: 10 November 2006 15:19 To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Friday Fun At 08:05 AM 11/10/2006, Breeden, Sara wrote: >Okay, here's the deal. Tell me, concisely, what would be the most >unlikely thing a histologist would hear from a pathologist? For >example, "No, please! Don't recut that broken slide - I can see just >fine through the cellophane tape". Send your innovative, imaginative, >and funny responses directly to me at sbreeden@nmda.nmsu.edu. This >could lead to a Very Funny Friday! "Start your engines!!" > > > >Sally Breeden, HT(ASCP) > >NM Dept. of Agriculture > >Veterinary Diagnostic Services > >PO Box 700 > >Albuquerque, NM 87106 > >505-841-2576 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet " I will always gross at 3mm or less just to help you out from now on, especially fatty specimens. I will even teach the residents to gross smaller!" Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From BMolinari <@t> heart.thi.tmc.edu Fri Nov 10 10:52:44 2006 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Nov 10 10:52:50 2006 Subject: [Histonet] Re: Cut resistant gloves In-Reply-To: <63984BC3A63FF542AF6EF0A237F38F4D7E4C9A@LIL.xRothGen.nhs.uk> Message-ID: Ouch! Besides our 2 Harleys I ride a Triumph Bonneville (antique restored) and we have a BSA we are rebuilding so am I exempt? Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: Friday, November 10, 2006 9:25 AM To: Kemlo Rogerson; RSRICHMOND@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Cut resistant gloves Agree (for a change). Harley's are big, ugly, sound like tractors, and invariably ridden by apes. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk] Sent: 10 November 2006 10:02 To: RSRICHMOND@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Cut resistant gloves Harley Davidson? Oh I see those big old bikes that are VERY VERY SLOW and don't go round corners. No I meant real bikes, Yamaha, Suzuki, Honda, good british bikes!!!! Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Habits of thinking need not be forever. One of the most significant findings in psychology in the last twenty years is that individuals can choose the way they think. --Martin Seligman This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From megantwomey <@t> hotmail.com Fri Nov 10 10:56:35 2006 From: megantwomey <@t> hotmail.com (megan twomey) Date: Fri Nov 10 10:56:48 2006 Subject: [Histonet] Help with immunohistochemistry in human embryos Message-ID: How do I remove myself from this list? I have e-mailed histonet.com but it hasn't worked? Any help is appreciated. Thank you ______________________________________________________________ From: "Amy Porter" To: "Hernan Aldana" , Subject: Re: [Histonet] Help with immunohistochemistry in human embryos Date: Fri, 10 Nov 2006 10:24:11 -0500 >You might want to try using a Heat Epitope Induced Retrieval >protocol. Most of Santa Cruz's antibodies will work with heat >retrieval. >Amy S. Porter, HT (ASCP) QIHC >Investigative HistoPathology Laboratory - Supervisor >2201 Biomedical Physical Sciences Bldg. Rm #2133 >East Lansing, MI 48824-3320 >Phone: (517) 355-6475 ext 1480 >Fax: (517) 432-1368 >Email: portera@msu.edu >Web: www.humanpathology.msu.edu >----- Original Message ----- From: "Hernan Aldana" > >To: >Sent: Thursday, November 09, 2006 10:45 AM >Subject: [Histonet] Help with immunohistochemistry in human embryos > > >Dear Histoneters >I need some advices. I'm working with human embryos between 7 and 14 >weeks. >we used Sonic Hedgehog Shh (C-18) and sc-1195 antibodies of Santa >Cruz >Biotechnology in paraffin sections of embryos fixed in >paraformadehyde. I >don't have good results. >Could anybody help me with some advice, concerning to every step of >my >technique? > >Thanks in advance > >Dr. Hernán Javier Aldana Marcos > >Prof. Titular Reg. Histología, Embriología y Biología Celular > > >Facultad de Medicina >Universidad de Morón >Argentina > >email altenrativo: hernanjavier@yahoo.com >http://www.ht.org.ar/educacion.htm > > > > > >-----Mensaje original----- >De: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] En nombre de >histonet-request@lists.utsouthwestern.edu >Enviado el: Domingo, 01 de Octubre de 2006 02:04 p.m. >Para: histonet@lists.utsouthwestern.edu >Asunto: Histonet Digest, Vol 35, Issue 1 > >Send Histonet mailing list submissions to >histonet@lists.utsouthwestern.edu > >To subscribe or unsubscribe via the World Wide Web, visit >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >or, via email, send a message with subject or body 'help' to >histonet-request@lists.utsouthwestern.edu > >You can reach the person managing the list at >histonet-owner@lists.utsouthwestern.edu > >When replying, please edit your Subject line so it is more specific >than "Re: Contents of Histonet digest..." > > >Today's Topics: > > 1. Job Opening (Luck, Greg D.) > 2. RE: Problem of Double immunoflouresence staining .. > (Melissa Gonzalez) > 3. The procedure that substitues xylene with mineral oil. > (PhiHo Hoang) > 4. Re: The procedure that substitues xylene with mineral oil. > (Rene J Buesa) > > >------------------------------------------------------------------ ---- > >Message: 1 >Date: Sat, 30 Sep 2006 12:01:02 -0700 >From: "Luck, Greg D." >Subject: [Histonet] Job Opening >To: >Cc: "Ringwood, James F." , "Wakabayashi, >Marlene T." , "Browning, Rachel M." > >Message-ID: ><6BB8BC4519AAB844B174FC739A679BBC50900C@IRMEXCH01.irm.inhs.org> >Content-Type: text/plain; charset="us-ascii" > >Hello All, > >Deaconess is a 300 bed acute care community medical center. We have >a >full time, M-F, day time position open. Must be HT(ASCP) certified >or >eligible. Duties include all routine histology functions in a >clinical >setting (i.e. embedding , cutting, special stains and frozen >sections). >IHC experience preferred. Due to a recent market adjustment by our >organization the salary range for this position has just increased >25-33% depending on years of experience. This is a golden >opportunity >for someone to improve on their current situation or to begin anew. >Anyone new to this field is encouraged to apply as well, for the >door is >wide open. You can apply on-line by going to our corporate website >www.empirehealth.org. The position is at Deaconess Medical Center >in >Spokane, WA www.deaconess-spokane.org. Please contact me directly >for >more details. Following is an additional web link to provide you >with >more info on Spokane and the greater Inland Northwest region >www.visitspokane.com. ("Near Perfect-Near Nature"). Thank you and >best >wishes, Greg > >Greg Luck, BS, HT(ASCP) >Anatomic Pathology Supervisor >Deaconess Medical Center >800 W. 5th Ave >Spokane, WA 99204 >Phone 509.473.7077 >Fax 509.473.7133 >luckg@empirehealth.org >www.deaconess-spokane.org > > > >------------------------------ > >Message: 2 >Date: Sun, 1 Oct 2006 08:40:44 -0700 >From: "Melissa Gonzalez" >Subject: [Histonet] RE: Problem of Double immunoflouresence staining >.. >To: >Message-ID: > >Content-Type: text/plain; charset="us-ascii" > >Sohail, >First, Perhaps you should try incubating each primary with >respective >secondary only, to determine how they look separately, and also make >sure each is working on its own. For example, you may not have your >aSMA >dilution correct. Also, do you have a positive control for TRITC to >make >sure you can detect any TRITC signal adequately? Once you have >appropriate signal from both, you can then make your cocktails. >Also, in >the future, I would recommend switching to Alexa Fluors from >Molecular >Probes, they really are great. In your instance you would use AF488 >(FITC) and AF 546 or 555 (TRITC). But that is just my 2 cents. > >Good luck, >Melissa > > > >Message: 4 >Date: Fri, 29 Sep 2006 13:06:27 -0700 (PDT) >From: sohail ejaz >Subject: [Histonet] Problem of Double immunoflouresence staining of >retinal vasculature >To: histonet@lists.utsouthwestern.edu >Message-ID: <20060929200627.949.qmail@web39506.mail.mud.yahoo.com> >Content-Type: text/plain; charset=iso-8859-1 > >Hello everybody > > I have a big problem of staining retina vasculature using a >cocktail >of two different antibodies. The problem is that i can only see >staining >with FITC but not with TRITC, hence i cant make a merger of both >FITC >and TRITC. > > Cocktail of primary antibodies include (1) Monoclonal Rabbit Anti >human vWF (2) Monoclonal mouse Anti alpha SMA and cocktail of >secondary >antibodies include (1) Anti rabbit FITC (2) Anti mouse TRITC > > Please let me know your commentc to slove this issue. > > Dr.Sohail > > > > > >------------------------------ > >Message: 3 >Date: Sun, 1 Oct 2006 11:47:56 -0400 >From: "PhiHo Hoang" >Subject: [Histonet] The procedure that substitues xylene with >mineral >oil. >To: >Message-ID: <005b01c6e570$f725b820$f300a8c0@ttth> >Content-Type: text/plain; charset="iso-8859-1" > > >Greetings, > >I am a new subscriber to this list and I am reading >the list archive. > >I came across a posting from Reni J. responding to >a request from Sharon: > > > >Sharon: > I am sending privately a procedure I have that substitutes xylene >with >mineral oil. It is 100 times less toxic than xylene and 3 times >cheaper. > Reni J. > >Sharon Allen exchange.hsc.mb.ca> wrote: > >Does anyone use xylol substitutes, if so how good are they? >We do CJD testing & "the powers that be" don't like to pay for >getting rid >of contaminated xylol. I don't want to have to start testing >different >substitutes, so any help would be greatly appreciated. >Thanks >Sharon >sallen <@t> hsc.mb.ca > > > >I am interested in this procedure. > >It is very much appreciated if I can get a copy >of that procedure. > >Best regards, > >PhiHo > > > > > >------------------------------ > >Message: 4 >Date: Sun, 1 Oct 2006 09:55:53 -0700 (PDT) >From: Rene J Buesa >Subject: Re: [Histonet] The procedure that substitues xylene with >mineral oil. >To: PhiHo Hoang , >histonet@lists.utsouthwestern.edu >Message-ID: <20061001165553.32345.qmail@web61219.mail.yahoo.com> >Content-Type: text/plain; charset=iso-8859-1 > >PhiHo: > I am attaching the procedure under separate cover. > Reni J. > >PhiHo Hoang wrote: > >Greetings, > >I am a new subscriber to this list and I am reading >the list archive. > >I came across a posting from Reni J. responding to >a request from Sharon: > > > >Sharon: >I am sending privately a procedure I have that substitutes xylene >with >mineral oil. It is 100 times less toxic than xylene and 3 times >cheaper. >Reni J. > >Sharon Allen exchange.hsc.mb.ca> wrote: > >Does anyone use xylol substitutes, if so how good are they? >We do CJD testing & "the powers that be" don't like to pay for >getting rid >of contaminated xylol. I don't want to have to start testing >different >substitutes, so any help would be greatly appreciated. >Thanks >Sharon >sallen <@t> hsc.mb.ca > > > >I am interested in this procedure. > >It is very much appreciated if I can get a copy >of that procedure. > >Best regards, > >PhiHo > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. >Great rates >starting at 1"/min. > >------------------------------ > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >End of Histonet Digest, Vol 35, Issue 1 >*************************************** > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ [1]Use your PC to make calls at very low rates References 1. http://g.msn.com/8HMBENUS/2749??PS=47575 From Terry.Marshall <@t> rothgen.nhs.uk Fri Nov 10 10:57:33 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Nov 10 10:59:25 2006 Subject: [Histonet] Fw: Funny Friday Message-ID: <63984BC3A63FF542AF6EF0A237F38F4D7E4CA0@LIL.xRothGen.nhs.uk> Perhaps he was thinking of the stain for rotor virus:-) Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Michelle D. Moore [mailto:tmhpath@amigo.net] Sent: 10 November 2006 16:49 To: Histonet Subject: [Histonet] Fw: Funny Friday This may not be unlikely to hear but we thought it was kind of funny at the time Our pathologist left us a note that asked us to do a "Helicopter stain" on a gastric specimen, so my tech at the time asked him if he wanted us to do that at the airport. Michelle D. Moore TMH Craig, CO _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pjfnefro <@t> duke.edu Fri Nov 10 11:44:21 2006 From: pjfnefro <@t> duke.edu (Pat Flannery) Date: Fri Nov 10 11:44:37 2006 Subject: [Histonet] Bikes and Organ Donors In-Reply-To: References: Message-ID: Thought I'd change the subject line since we've gone way past gloves (does that mean the gloves are off?). Betsy, you've got me curious now and I'm going to show my ignorance here, but what the heck's a BSA? BMW I know, but BSA sounds like lyophilized cow serum, not a motorcycle. -Pat Flannery On Nov 10, 2006, at 11:52 AM, Molinari, Betsy wrote: > Ouch! Besides our 2 Harleys I ride a Triumph Bonneville (antique > restored) and we have a BSA we are rebuilding so am I exempt? > > Betsy Molinari HT (ASCP) > Texas Heart Institute > Cardiovascular Pathology > 6770 Bertner Ave. > Houston,TX 77030 > 832-355-6524 > 832-355-6812 (fax) > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Marshall > Terry Dr,Consultant Histopathologist > Sent: Friday, November 10, 2006 9:25 AM > To: Kemlo Rogerson; RSRICHMOND@aol.com; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Cut resistant gloves > > Agree (for a change). > Harley's are big, ugly, sound like tractors, and invariably ridden by > apes. > > Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path > Consultant Pathologist > Rotherham General Hospital > South Yorkshire > England > terry.marshall@rothgen.nhs.uk > > -----Original Message----- > From: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk] > Sent: 10 November 2006 10:02 > To: RSRICHMOND@aol.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Cut resistant gloves > > > Harley Davidson? Oh I see those big old bikes that are VERY VERY SLOW > and don't go round corners. No I meant real bikes, Yamaha, Suzuki, > Honda, good british bikes!!!! > > > Kemlo Rogerson > Pathology Manager > Ext 3311 > DD 01934 647057 > Mob 07749 754194 > Pager 07659 597107 > E-Mail: kemlo.rogerson@nhs.net From tkngflght <@t> yahoo.com Fri Nov 10 10:07:18 2006 From: tkngflght <@t> yahoo.com (Cheryl) Date: Fri Nov 10 11:54:10 2006 Subject: [Histonet] Seeking techs with specific skills for temp and regular job openings. In-Reply-To: Message-ID: <415244.78294.qm@web50906.mail.yahoo.com> Hi All-- Happy Friday! You probably know who I am and that I work as a tech as well as employing temporary techs for labs as a recruiter.... I'm looking for a couple of special skill sets--please call if you want more information than I post below. My favorite part of this kind of work is meeting great new people! I pick up the phones seven days a week until about 10PM CST. If you know someone not on the histonet, I appreciate referrals and the bonus program applies. 1. We need a Histotech with grossing skills. If you aren't sure if you are qualified but can gross even small tissue, give me a call. 2. A Diener--someone interested in learning histology if they don't already. This is an amazing opportunity for expanding your skills and learning a LOT while being able to teach a little, as well. 3. A Med Tech (not necessarily registered), including new grads, with full micro experience. 4. And of course--always open to helping a good tech find a permanent job that fits and helping folks try on temp/travel. We're a good first company to temp for as I temp frequently and know the ropes first-hand (the good bits AND the icky ones). I'd like to talk with you if you're even a little curious or aren't ready for months---call and ask the questions you need to make the decision for yourself-- information is GOOD! Thank you for sharing the wealth of knowledge I find on here daily--the jokes have been a hoot! Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one Tech at a time. 281.883.7704 c 281.852.9457 o admin@fullstaff.org Sign up for the FREE monthly newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. From Jackie.O'Connor <@t> abbott.com Fri Nov 10 11:54:23 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Nov 10 11:54:55 2006 Subject: [Histonet] Something new - -I'm waiting In-Reply-To: Message-ID: OK - so I read my own question and it doesn't make enough sense. Maybe I can elaborate a bit - Is it possible to administer a fluorescent probe in vivo (Like GFP) visualize that probe in vivo, then harvest tissues and be able to see that probe in frozen sections using a fluorescent scope? "Jackie M O'Connor" Sent by: histonet-bounces@lists.utsouthwestern.edu 11/10/2006 09:49 AM To histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu cc Subject [Histonet] Something new Can someone advise me the best way to preserve (Liguid nitrogen, OCT, fixed?) and visualize tissues from animals which have been administered (in vivo) a fluorescent probe? Thanks, Jackie O' on a crummy Friday morning fighting fires again as usual. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pjfnefro <@t> duke.edu Fri Nov 10 11:54:48 2006 From: pjfnefro <@t> duke.edu (Pat Flannery) Date: Fri Nov 10 11:55:37 2006 Subject: [Histonet] Bikes and Organ Donors In-Reply-To: References: Message-ID: <79462740-8EB6-42D9-949A-F74458B1F3C2@duke.edu> Answering my own question here, but I think I just figured out what a BSA is: Birmingham Small Arms merged with Norton International and the BSA motorcycle works was spun off from them. I guess I just never saw a BSA over here. Did they go OOB or are they still around (all the pics on the net seem to be old/classic bikes)? -Pat Flannery On Nov 10, 2006, at 12:44 PM, Pat Flannery wrote: > Thought I'd change the subject line since we've gone way past > gloves (does that mean the gloves are off?). > > Betsy, you've got me curious now and I'm going to show my ignorance > here, but what the heck's a BSA? BMW I know, but BSA sounds like > lyophilized cow serum, not a motorcycle. > > -Pat Flannery > > > On Nov 10, 2006, at 11:52 AM, Molinari, Betsy wrote: > >> Ouch! Besides our 2 Harleys I ride a Triumph Bonneville (antique >> restored) and we have a BSA we are rebuilding so am I exempt? >> >> Betsy Molinari HT (ASCP) >> Texas Heart Institute >> Cardiovascular Pathology >> 6770 Bertner Ave. >> Houston,TX 77030 >> 832-355-6524 >> 832-355-6812 (fax) From timothy.macatee <@t> med.nyu.edu Fri Nov 10 11:55:19 2006 From: timothy.macatee <@t> med.nyu.edu (Timothy Macatee) Date: Fri Nov 10 11:59:07 2006 Subject: [Histonet] Fw: Funny Friday In-Reply-To: <63984BC3A63FF542AF6EF0A237F38F4D7E4CA0@LIL.xRothGen.nhs.uk> Message-ID: "I just learned grossing from watching CSI, Miami" -- Tim Macatee Research Histology Core New York University School of Medicine 550 First Ave. Department of Pathology. Medical Science Building - Room 521 New York, N.Y. 10016 (212) 263-3888 From mcauliff <@t> umdnj.edu Fri Nov 10 11:59:47 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Nov 10 11:59:09 2006 Subject: [Histonet] Bikes and Organ Donors In-Reply-To: References: Message-ID: <4554BE13.8060409@umdnj.edu> BSA = Birmingham Small Arms, I believe. Geoff Pat Flannery wrote: > Thought I'd change the subject line since we've gone way past gloves > (does that mean the gloves are off?). > > Betsy, you've got me curious now and I'm going to show my ignorance > here, but what the heck's a BSA? BMW I know, but BSA sounds like > lyophilized cow serum, not a motorcycle. > > -Pat Flannery > > > On Nov 10, 2006, at 11:52 AM, Molinari, Betsy wrote: > >> Ouch! Besides our 2 Harleys I ride a Triumph Bonneville (antique >> restored) and we have a BSA we are rebuilding so am I exempt? >> >> Betsy Molinari HT (ASCP) >> Texas Heart Institute >> Cardiovascular Pathology >> 6770 Bertner Ave. >> Houston,TX 77030 >> 832-355-6524 >> 832-355-6812 (fax) >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >> Marshall >> Terry Dr,Consultant Histopathologist >> Sent: Friday, November 10, 2006 9:25 AM >> To: Kemlo Rogerson; RSRICHMOND@aol.com; >> histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] Re: Cut resistant gloves >> >> Agree (for a change). >> Harley's are big, ugly, sound like tractors, and invariably ridden by >> apes. >> >> Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path >> Consultant Pathologist >> Rotherham General Hospital >> South Yorkshire >> England >> terry.marshall@rothgen.nhs.uk >> >> -----Original Message----- >> From: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk] >> Sent: 10 November 2006 10:02 >> To: RSRICHMOND@aol.com; histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] Re: Cut resistant gloves >> >> >> Harley Davidson? Oh I see those big old bikes that are VERY VERY SLOW >> and don't go round corners. No I meant real bikes, Yamaha, Suzuki, >> Honda, good british bikes!!!! >> >> >> Kemlo Rogerson >> Pathology Manager >> Ext 3311 >> DD 01934 647057 >> Mob 07749 754194 >> Pager 07659 597107 >> E-Mail: kemlo.rogerson@nhs.net > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From hymclab <@t> hyhc.com Fri Nov 10 12:02:46 2006 From: hymclab <@t> hyhc.com (hymclab) Date: Fri Nov 10 11:59:43 2006 Subject: [Histonet] Re: Cut resistant gloves Message-ID: I don't know, I sure like the deep roar of my Harley and the feel of my powerful horsepower compared to the whine of the foreign bikes!!!! And I don't think I look like an ape!!!!! By the way, I'm a 43 year old female and proud to have my own Harley!!!! Dawn -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: Friday, November 10, 2006 9:25 AM To: Kemlo Rogerson; RSRICHMOND@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Cut resistant gloves Agree (for a change). Harley's are big, ugly, sound like tractors, and invariably ridden by apes. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk] Sent: 10 November 2006 10:02 To: RSRICHMOND@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Cut resistant gloves Harley Davidson? Oh I see those big old bikes that are VERY VERY SLOW and don't go round corners. No I meant real bikes, Yamaha, Suzuki, Honda, good british bikes!!!! Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Habits of thinking need not be forever. One of the most significant findings in psychology in the last twenty years is that individuals can choose the way they think. --Martin Seligman This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From BMolinari <@t> heart.thi.tmc.edu Fri Nov 10 12:06:27 2006 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Nov 10 12:06:35 2006 Subject: [Histonet] Bikes and Organ Donors In-Reply-To: Message-ID: Birmingham Small Arms Company.Founded in 1861 to supply arms to the British government during the Crimean War. They branched out and started building bicycles, automobiles and then motorcycles.They were eventually bought out by Triumph Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pat Flannery Sent: Friday, November 10, 2006 11:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bikes and Organ Donors Thought I'd change the subject line since we've gone way past gloves (does that mean the gloves are off?). Betsy, you've got me curious now and I'm going to show my ignorance here, but what the heck's a BSA? BMW I know, but BSA sounds like lyophilized cow serum, not a motorcycle. -Pat Flannery On Nov 10, 2006, at 11:52 AM, Molinari, Betsy wrote: > Ouch! Besides our 2 Harleys I ride a Triumph Bonneville (antique > restored) and we have a BSA we are rebuilding so am I exempt? > > Betsy Molinari HT (ASCP) > Texas Heart Institute > Cardiovascular Pathology > 6770 Bertner Ave. > Houston,TX 77030 > 832-355-6524 > 832-355-6812 (fax) > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Marshall > Terry Dr,Consultant Histopathologist > Sent: Friday, November 10, 2006 9:25 AM > To: Kemlo Rogerson; RSRICHMOND@aol.com; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Cut resistant gloves > > Agree (for a change). > Harley's are big, ugly, sound like tractors, and invariably ridden by > apes. > > Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path > Consultant Pathologist > Rotherham General Hospital > South Yorkshire > England > terry.marshall@rothgen.nhs.uk > > -----Original Message----- > From: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk] > Sent: 10 November 2006 10:02 > To: RSRICHMOND@aol.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Cut resistant gloves > > > Harley Davidson? Oh I see those big old bikes that are VERY VERY SLOW > and don't go round corners. No I meant real bikes, Yamaha, Suzuki, > Honda, good british bikes!!!! > > > Kemlo Rogerson > Pathology Manager > Ext 3311 > DD 01934 647057 > Mob 07749 754194 > Pager 07659 597107 > E-Mail: kemlo.rogerson@nhs.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BJDewe <@t> aol.com Fri Nov 10 12:08:16 2006 From: BJDewe <@t> aol.com (BJDewe@aol.com) Date: Fri Nov 10 12:08:42 2006 Subject: [Histonet] Animal tissue control slides Message-ID: Hi All, Our company is now producing high quality FFPE animal slides for use as control slides for research and veterinary pathology. All species available. No request too outrageous! See http://www.innvx.com/ for further information...any questions don't hesitate to ask! From BMolinari <@t> heart.thi.tmc.edu Fri Nov 10 12:11:57 2006 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Nov 10 12:12:03 2006 Subject: [Histonet] Bikes and Organ Donors In-Reply-To: Message-ID: Correction..BSA bought out Triumph. There were plans to combine Norton,Triumph and BSA but that failed. It is now owned by the Regal BSA Group and they turn out some retro bikes on a limited basis. Our is '71 650CC Lightening. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Friday, November 10, 2006 12:06 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Bikes and Organ Donors Birmingham Small Arms Company.Founded in 1861 to supply arms to the British government during the Crimean War. They branched out and started building bicycles, automobiles and then motorcycles.They were eventually bought out by Triumph Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pat Flannery Sent: Friday, November 10, 2006 11:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bikes and Organ Donors Thought I'd change the subject line since we've gone way past gloves (does that mean the gloves are off?). Betsy, you've got me curious now and I'm going to show my ignorance here, but what the heck's a BSA? BMW I know, but BSA sounds like lyophilized cow serum, not a motorcycle. -Pat Flannery On Nov 10, 2006, at 11:52 AM, Molinari, Betsy wrote: > Ouch! Besides our 2 Harleys I ride a Triumph Bonneville (antique > restored) and we have a BSA we are rebuilding so am I exempt? > > Betsy Molinari HT (ASCP) > Texas Heart Institute > Cardiovascular Pathology > 6770 Bertner Ave. > Houston,TX 77030 > 832-355-6524 > 832-355-6812 (fax) > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Marshall > Terry Dr,Consultant Histopathologist > Sent: Friday, November 10, 2006 9:25 AM > To: Kemlo Rogerson; RSRICHMOND@aol.com; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Cut resistant gloves > > Agree (for a change). > Harley's are big, ugly, sound like tractors, and invariably ridden by > apes. > > Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path > Consultant Pathologist > Rotherham General Hospital > South Yorkshire > England > terry.marshall@rothgen.nhs.uk > > -----Original Message----- > From: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk] > Sent: 10 November 2006 10:02 > To: RSRICHMOND@aol.com; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Cut resistant gloves > > > Harley Davidson? Oh I see those big old bikes that are VERY VERY SLOW > and don't go round corners. No I meant real bikes, Yamaha, Suzuki, > Honda, good british bikes!!!! > > > Kemlo Rogerson > Pathology Manager > Ext 3311 > DD 01934 647057 > Mob 07749 754194 > Pager 07659 597107 > E-Mail: kemlo.rogerson@nhs.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pjfnefro <@t> duke.edu Fri Nov 10 12:12:26 2006 From: pjfnefro <@t> duke.edu (Pat Flannery) Date: Fri Nov 10 12:12:35 2006 Subject: [Histonet] Re:Bikes and Organ Donors In-Reply-To: <79462740-8EB6-42D9-949A-F74458B1F3C2@duke.edu> References: <79462740-8EB6-42D9-949A-F74458B1F3C2@duke.edu> Message-ID: While I'm asking, what's the difference between a BSA and a Norton or Triumph, since they seem to be made by the same folks (according to Wikipedia anyway)? Is it a Ford/Mercury/Lincoln type of thing or did one "become" the other at some point? My apologies to the rest of the Histonetters for whom this is pretty off-topic, but we've entered into an area near and dear to my heart (and wallet). I'll return you to your regularly-scheduled discussions of Histology after this post. Anyone willing to take this off-list is welcome to continue, though. -Pat Flannery pjfnefro@duke.edu On Nov 10, 2006, at 12:54 PM, Pat Flannery wrote: > Answering my own question here, but I think I just figured out what > a BSA is: Birmingham Small Arms merged with Norton International > and the BSA motorcycle works was spun off from them. I guess I > just never saw a BSA over here. Did they go OOB or are they still > around (all the pics on the net seem to be old/classic bikes)? > > -Pat Flannery > > On Nov 10, 2006, at 12:44 PM, Pat Flannery wrote: > >> Thought I'd change the subject line since we've gone way past >> gloves (does that mean the gloves are off?). >> >> Betsy, you've got me curious now and I'm going to show my >> ignorance here, but what the heck's a BSA? BMW I know, but BSA >> sounds like lyophilized cow serum, not a motorcycle. >> >> -Pat Flannery >> >> >> On Nov 10, 2006, at 11:52 AM, Molinari, Betsy wrote: >> >>> Ouch! Besides our 2 Harleys I ride a Triumph Bonneville (antique >>> restored) and we have a BSA we are rebuilding so am I exempt? >>> >>> Betsy Molinari HT (ASCP) >>> Texas Heart Institute >>> Cardiovascular Pathology >>> 6770 Bertner Ave. >>> Houston,TX 77030 >>> 832-355-6524 >>> 832-355-6812 (fax) From SHARON.OSBORN <@t> SPCORP.COM Fri Nov 10 12:17:31 2006 From: SHARON.OSBORN <@t> SPCORP.COM (Osborn, Sharon) Date: Fri Nov 10 12:17:42 2006 Subject: [Histonet] Bikes Message-ID: <9A919A5D70313A4D9C56A02571087408C54532@kenmsg40.us.schp.com> Kemlo, Suzuki, Yamaha and Honda are all Japanese companies. How are the British involved? sharon osborn SP BioPharma Palo Alto, CA --- Kemlo Rogerson wrote: > Harley Davidson? Oh I see those big old bikes that > are VERY VERY SLOW > and don't go round corners. No I meant real bikes, > Yamaha, Suzuki, > Honda, good british bikes!!!! ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From pjfnefro <@t> duke.edu Fri Nov 10 12:25:25 2006 From: pjfnefro <@t> duke.edu (Pat Flannery) Date: Fri Nov 10 12:25:35 2006 Subject: [Histonet] Re: Cut resistant gloves In-Reply-To: References: Message-ID: <1365E1E9-C621-4641-B2B0-F1ACE5CC2D6E@duke.edu> Watch it, Dawn. Not all foreign bikes are whiners (or wieners, for that matter). My Vulcan doesn't sound like any mosquito I've ever come across (and I never cared all that much for potatoes anyway). All seriousness aside, the main thing is not *what* you ride, but *that* you ride, and that you keep the rubber side down as much as possible (to avoid the whole Organ Donor thing). -Pat On Nov 10, 2006, at 1:02 PM, hymclab wrote: > I don't know, I sure like the deep roar of my Harley and the feel > of my > powerful horsepower compared to the whine of the foreign bikes!!!! > And I > don't think I look like an ape!!!!! > By the way, I'm a 43 year old female and proud to have my own > Harley!!!! > > Dawn > > -----Original Message----- > From: Marshall Terry Dr, Consultant Histopathologist > [mailto:Terry.Marshall@rothgen.nhs.uk] > Sent: Friday, November 10, 2006 9:25 AM > To: Kemlo Rogerson; RSRICHMOND@aol.com; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Cut resistant gloves > > Agree (for a change). > Harley's are big, ugly, sound like tractors, and invariably ridden > by apes. > > Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant > Pathologist Rotherham General Hospital South Yorkshire England > terry.marshall@rothgen.nhs.uk From GDawson <@t> dynacaremilwaukee.com Fri Nov 10 12:30:12 2006 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Fri Nov 10 12:30:22 2006 Subject: [Histonet] Mystery Fixative Message-ID: All, Our AP supervisor is looking for an older fixative that she used to use to revitalize staining on tissue that had been sitting in formalin for months. She seems to think it is called "Yori's, Youri's or Jori's" fixative and they used to make it on-site back in the day but the recipe has long since been lost. Any Help Would be Greatly Appreciated, Glen Dawson IHC Manager Milwaukee, WI From debbiekeith <@t> cox.net Fri Nov 10 12:31:52 2006 From: debbiekeith <@t> cox.net (Debbie Keith) Date: Fri Nov 10 12:32:03 2006 Subject: [Histonet] Re: Cut resistant gloves In-Reply-To: <1365E1E9-C621-4641-B2B0-F1ACE5CC2D6E@duke.edu> References: Message-ID: <5.2.0.9.0.20061110112731.02ecf1c8@pop.central.cox.net> At 01:25 PM 11/10/2006 -0500, you wrote: >Watch it, Dawn. Not all foreign bikes are whiners (or wieners, for >that matter). My Vulcan doesn't sound like any mosquito I've ever >come across (and I never cared all that much for potatoes anyway). >All seriousness aside, the main thing is not *what* you ride, but >*that* you ride, and that you keep the rubber side down as much as >possible (to avoid the whole Organ Donor thing). > >-Pat my daytona sounds different than all of 'em. the sweet growl of a triple will ruin you for anything else. :) that being said... i love my gsxr 750 that i ride on the track. i love the gixxer so much i penned haiku to express that love in the form of metered art.... gixxer i love thee sweet is your seat and styling sweet suzuki love and sweet seven-fitty engine so predictable sweetly flick-able -- No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.1.409 / Virus Database: 268.14.2/528 - Release Date: 11/10/2006 From Janet.Bonner <@t> FLHOSP.ORG Fri Nov 10 12:51:01 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Nov 10 12:51:52 2006 Subject: [Histonet] Fw: Funny Friday References: Message-ID: <5F31F38C96781A4FBE3196EBC22D478004A6A2@fhosxchmb006.ADVENTISTCORP.NET> ...And exactly what did you learn? ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Timothy Macatee Sent: Fri 11/10/2006 12:55 PM To: Marshall Terry Dr, Consultant Histopathologist; Michelle D. Moore; Histonet Subject: Re: [Histonet] Fw: Funny Friday "I just learned grossing from watching CSI, Miami" -- Tim Macatee Research Histology Core New York University School of Medicine 550 First Ave. Department of Pathology. Medical Science Building - Room 521 New York, N.Y. 10016 (212) 263-3888 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From llewllew <@t> shaw.ca Fri Nov 10 12:52:36 2006 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Fri Nov 10 12:53:05 2006 Subject: [Histonet] Bikes and Organ Donors References: Message-ID: <000e01c704f9$63534d80$130e4246@yourlk4rlmsu> BSA was Birmingham Small Arm originally a gun manufacturer and made the most powerful air rifles and pistols available (and still do). They branched out to make motor bikes and motor scooters, including the Golden Flash 500cc twin, of which I had one hooked up to a sidecar. Try taking a corner fast on that combination if you want a "thrill", but wear plastic underwear! They did own Triumph motorcycles at one time. Bryan Llewellyn ----- Original Message ----- From: "Pat Flannery" To: Sent: Friday, November 10, 2006 9:44 AM Subject: [Histonet] Bikes and Organ Donors > Thought I'd change the subject line since we've gone way past gloves > (does that mean the gloves are off?). > > Betsy, you've got me curious now and I'm going to show my ignorance here, > but what the heck's a BSA? BMW I know, but BSA sounds like lyophilized > cow serum, not a motorcycle. > > -Pat Flannery > > > On Nov 10, 2006, at 11:52 AM, Molinari, Betsy wrote: > >> Ouch! Besides our 2 Harleys I ride a Triumph Bonneville (antique >> restored) and we have a BSA we are rebuilding so am I exempt? >> >> Betsy Molinari HT (ASCP) >> Texas Heart Institute >> Cardiovascular Pathology >> 6770 Bertner Ave. >> Houston,TX 77030 >> 832-355-6524 >> 832-355-6812 (fax) >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall >> Terry Dr,Consultant Histopathologist >> Sent: Friday, November 10, 2006 9:25 AM >> To: Kemlo Rogerson; RSRICHMOND@aol.com; >> histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] Re: Cut resistant gloves >> >> Agree (for a change). >> Harley's are big, ugly, sound like tractors, and invariably ridden by >> apes. >> >> Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path >> Consultant Pathologist >> Rotherham General Hospital >> South Yorkshire >> England >> terry.marshall@rothgen.nhs.uk >> >> -----Original Message----- >> From: Kemlo Rogerson [mailto:Kemlo.Rogerson@waht.swest.nhs.uk] >> Sent: 10 November 2006 10:02 >> To: RSRICHMOND@aol.com; histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] Re: Cut resistant gloves >> >> >> Harley Davidson? Oh I see those big old bikes that are VERY VERY SLOW >> and don't go round corners. No I meant real bikes, Yamaha, Suzuki, >> Honda, good british bikes!!!! >> >> >> Kemlo Rogerson >> Pathology Manager >> Ext 3311 >> DD 01934 647057 >> Mob 07749 754194 >> Pager 07659 597107 >> E-Mail: kemlo.rogerson@nhs.net > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Janet.Bonner <@t> FLHOSP.ORG Fri Nov 10 12:52:03 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Nov 10 12:54:03 2006 Subject: [Histonet] paraffin time References: <63984BC3A63FF542AF6EF0A237F38F4D7E4C9C@LIL.xRothGen.nhs.uk> Message-ID: <5F31F38C96781A4FBE3196EBC22D478004A6A3@fhosxchmb006.ADVENTISTCORP.NET> I believe you are looking in the wrong place for the definition. My cookbook says, " When meat fibers are blackened and do not retain the sauce". Julia Childs @:) ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Marshall Terry Dr,Consultant Histopathologist Sent: Fri 11/10/2006 11:22 AM To: Margiotta, Michele; histonet@pathology.swmed.edu Subject: RE: [Histonet] paraffin time This "cooked" look crops up with monotonous regularity, but I have yet to see it defined in a comprehensible fashion, or seen a photograph upon which we can all agree is a "cooked look". Since heat is a satisfactory form of fixation, how can something look cooked? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Margiotta, Michele [mailto:MMargiotta@bmhmc.org] Sent: 10 November 2006 16:08 To: histonet@pathology.swmed.edu Subject: [Histonet] paraffin time Hi All, Wondering if anyone has an idea how long small bxs. can remain in the last wax station after the processing is completed. We had a problem with that today and a lot of our bxs look "cooked". Thanks! Michele This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to which they are addressed. This communication may contain material protected by the attorney-client privilege. If you are not the intended recipient or the person responsible for delivering the e-mail to the intended recipient, be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the sender via return e-mail or call Brookhaven Memorial Hospital Medical Center at (631) 654-7282. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From Rcartun <@t> harthosp.org Fri Nov 10 13:01:15 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Nov 10 13:02:00 2006 Subject: [Histonet] Animal tissue control slides In-Reply-To: References: Message-ID: <4554862B0200007700002C77@hcnwgwds01.hh.chs> I hope you obtained the animals' permission to sell their tissue! Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> 11/10/06 1:08 PM >>> Hi All, Our company is now producing high quality FFPE animal slides for use as control slides for research and veterinary pathology. All species available. No request too outrageous! See http://www.innvx.com/ for further information...any questions don't hesitate to ask! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hymclab <@t> hyhc.com Fri Nov 10 13:11:35 2006 From: hymclab <@t> hyhc.com (hymclab) Date: Fri Nov 10 13:08:26 2006 Subject: [Histonet] Re: Cut resistant gloves Message-ID: OK, I should have (but didn't because I didn't want to offend anyone) say crotch rockets are the whiners. We do ride with quite a few other people that have Hondas, Yamahas and other makes and models that are not whiners. I agree with you whole heartedly----it doesn't matter what you ride!!!! Dawn -----Original Message----- From: Pat Flannery [mailto:pjfnefro@duke.edu] Sent: Friday, November 10, 2006 12:25 PM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Cut resistant gloves Watch it, Dawn. Not all foreign bikes are whiners (or wieners, for that matter). My Vulcan doesn't sound like any mosquito I've ever come across (and I never cared all that much for potatoes anyway). All seriousness aside, the main thing is not *what* you ride, but *that* you ride, and that you keep the rubber side down as much as possible (to avoid the whole Organ Donor thing). -Pat On Nov 10, 2006, at 1:02 PM, hymclab wrote: > I don't know, I sure like the deep roar of my Harley and the feel of > my powerful horsepower compared to the whine of the foreign bikes!!!! > And I > don't think I look like an ape!!!!! > By the way, I'm a 43 year old female and proud to have my own > Harley!!!! > > Dawn > > -----Original Message----- > From: Marshall Terry Dr, Consultant Histopathologist > [mailto:Terry.Marshall@rothgen.nhs.uk] > Sent: Friday, November 10, 2006 9:25 AM > To: Kemlo Rogerson; RSRICHMOND@aol.com; > histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Cut resistant gloves > > Agree (for a change). > Harley's are big, ugly, sound like tractors, and invariably ridden by > apes. > > Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant > Pathologist Rotherham General Hospital South Yorkshire England > terry.marshall@rothgen.nhs.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From Adam.T.Anthony <@t> kp.org Fri Nov 10 13:09:19 2006 From: Adam.T.Anthony <@t> kp.org (Adam.T.Anthony@kp.org) Date: Fri Nov 10 13:11:33 2006 Subject: [Histonet] Histotech position available Message-ID: Kaiser Permanente San Francisco is currently seeking a full time histotechnologist experienced in immunohistochemistry. This individual must have 2-3 years recent experience in the performance of routine histology tasks including, microtomy, special stains and immunohistochemistry. We are seeking someone with leadership skills, first-rate communication and interpersonal skills, and the ability to work as a team member. Stable work and attendance record is required. Experience preparing and cutting tissue arrays is welcomed. Come be part of a great organization settled in the midst of one of America's greatest cities. Kaiser Permanente San Francisco offers an exceptional working environment with opportunities for professional growth and development. We're a growing, high volume laboratory at the forefront of innovation. Competitive salary and benefit package that includes medical, dental and vision benefits, paid time off and 401(k). Up to $35.00/hr, depending on experience and certification. Apply for histotech I or histotech II position (histotech II requires ASCP certification as HT or HTL). The lab is surrounded by limitless cultural attractions, restaurants, and museums! E-mail resume to adam.t.anthony@kp.org or fax your resume with a coversheet to 415-833-3871, attention Adam Anthony Adam Anthony Manager, Pathology & Regional Immunohistochemistry Kaiser Permanente Medical Center San Francisco From timothy.macatee <@t> med.nyu.edu Fri Nov 10 13:09:28 2006 From: timothy.macatee <@t> med.nyu.edu (Timothy Macatee) Date: Fri Nov 10 13:13:11 2006 Subject: [Histonet] Fw: Funny Friday In-Reply-To: <5F31F38C96781A4FBE3196EBC22D478004A6A2@fhosxchmb006.ADVENTISTCORP.NET> Message-ID: I learned that getting a H&E stained slide takes less than 10 minutes. On 11/10/06 1:51 PM, "Bonner, Janet" wrote: > ...And exactly what did you learn? > > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Timothy Macatee > Sent: Fri 11/10/2006 12:55 PM > To: Marshall Terry Dr, Consultant Histopathologist; Michelle D. Moore; > Histonet > Subject: Re: [Histonet] Fw: Funny Friday > > "I just learned grossing from watching CSI, Miami" > > -- > Tim Macatee > Research Histology Core > New York University School of Medicine > 550 First Ave. > Department of Pathology. > Medical Science Building - Room 521 > New York, N.Y. 10016 > (212) 263-3888 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ======================================================= > The information contained in this message may be privileged and/or > confidential > and protected from disclosure. If the reader of this message is not the > intended > recipient or an employee or agent responsible for delivering this message to > the > intended recipient, you are hereby notified that any dissemination, > distribution > or copying of this communication is strictly prohibited. If you have received > this > communication in error, please notify the sender immediately by replying to > this > message and deleting the material from any computer. > ======================================================= > -- Tim Macatee Research Histology Core New York University School of Medicine 550 First Ave. Department of Pathology. Medical Science Building - Room 521 New York, N.Y. 10016 (212) 263-3888 From j.potas <@t> gmail.com Fri Nov 10 13:13:59 2006 From: j.potas <@t> gmail.com (Jason Potas) Date: Fri Nov 10 13:14:24 2006 Subject: [Histonet] remove me from the list Message-ID: Please remove me from the histonet list cheers jason -- ______________________________________ Jason Potas (PhD) Laboratorio de Neurobiologia Celular e Molecular Instituto de Biofisica Carlos Chagas Filho - IBCCF Universidade Federal do Rio de Janeiro CCS bloco J sala J1-029 Ilha do Fundao 21941-590 Rio de Janeiro, RJ - Brasil Tel: +5521 2280 4694 +5521 2562 6554 Email: jason@biof.ufrj.br From froyer <@t> bitstream.net Fri Nov 10 13:21:12 2006 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Nov 10 13:21:52 2006 Subject: [Histonet] Friday Fun In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEF153@sjhaexc02.sjha.org> Message-ID: <000601c704fd$62a9a330$7701a80a@Ford> OH MY! Now you are really bringing back the memories of the old days. Our actual "End of Day" duty list in the late 60's, posted on the bulletin board, read something like this... 1. Remove and store microtome knifes. 2. Clean all areas of paraffin shavings. 3. Turn off water baths and embedding center. 4. Replace lids on stain and reagent containers. 5. Empty all ASH TRAYS. (!) 6. etc. 7. etc. Etc... Each bench had its own ash tray. Can you believe it?? The only place we were NOT allowed to smoke was in the room with the Technicon Duo processor. Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, November 10, 2006 10:38 AM To: Edwards, R.E.; Pamela Marcum; Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Friday Fun as ashes were flicked into the trash can full of paraffin scraps and xylene soaked gauze..... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Edwards, R.E. Sent: Friday, November 10, 2006 11:36 AM To: Edwards, R.E.; Pamela Marcum; Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Friday Fun and heard in the good old days...."Can you hold the cigarette for me I need two hands for this" -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: 10 November 2006 15:19 To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Friday Fun At 08:05 AM 11/10/2006, Breeden, Sara wrote: >Okay, here's the deal. Tell me, concisely, what would be the most >unlikely thing a histologist would hear from a pathologist? For >example, "No, please! Don't recut that broken slide - I can see just >fine through the cellophane tape". Send your innovative, imaginative, >and funny responses directly to me at sbreeden@nmda.nmsu.edu. This >could lead to a Very Funny Friday! "Start your engines!!" > > > >Sally Breeden, HT(ASCP) > >NM Dept. of Agriculture > >Veterinary Diagnostic Services > >PO Box 700 > >Albuquerque, NM 87106 > >505-841-2576 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet " I will always gross at 3mm or less just to help you out from now on, especially fatty specimens. I will even teach the residents to gross smaller!" Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From Jessica <@t> medstaffservices.com Fri Nov 10 13:21:57 2006 From: Jessica <@t> medstaffservices.com (Jessica Hirsch) Date: Fri Nov 10 13:22:08 2006 Subject: [Histonet] Please post message Message-ID: ____________Please Post the Positions Below to your Histonet Digest_________________ CURRENT EMPLOYMENT OPPROTUNITIES (HISTOLOGY, IMMUNOHISTOCHEMISTRY & PATHOLOGY) For immediate consideration for any position: Send resumes to Jessica@medstaffservices.com OR fax to 732.238.2152 For questions call: Jessica Hirsch at 732.238.6050 ext.58 I. Clarksburg, Maryland (Large Nationwide Clinical Labratory (division of BioReference)) 1. Histology Supervisor: Hours: Monday - Friday 12 midnight - 8:30am Pay: $25/hr + 10% night differential = $27.50/hr Benefits: Medical, Dental, 401k 2. Histology Technologist Hours: Monday - Friday 12 midnight - 8:30am Pay: $25/hr + 10% night differential = $27.50/hr Benefits: Medical, Dental, 401k 3. Grossers Hours: Monday - Friday 7pm-11:30pm Pay: $30/hr II. Elmwood Park, New Jersey ( Nationwide Clinical Laboratory) 1. Histology Technologist ( 2 positions available, immediate start ) Hours: Monday - Friday, 9:00pm - 5:30am Pay: Negotiable Benefits: Medical, Dental, 401k 2. ImmunoHistoChemistry Supervisor Hours: Monday - Friday, 7am or 8am - 4pm or 5pm Pay: Negotiable Benefits: Medical, Dental, 401k III. New York City, New York ( large world renowned Cancer Hospital ) 1. Pathologist Assistant Hours: Monday - Friday, Day shift, Full-Time Pay: $60k - $80k based on experience Benefits: Major Medical, Dental, Vision, 401k, etc. 2. Histology Technologist Hours: Monday - Friday (shift to be announced) Pay: negotiable Benefits: Medical, Dental, 401k For immediate consideration for any position: Send resumes to Jessica@medstaffservices.com OR fax to 732.238.2152 For questions call: Jessica Hirsch at 732.238.6050 ext.58 From sbreeden <@t> nmda.nmsu.edu Fri Nov 10 13:23:08 2006 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Nov 10 13:23:16 2006 Subject: [Histonet] FUNNY SUMMARY Message-ID: <4D14F0FC9316DD41972D5F03C070908B0DB33D@nmdamailsvr.nmda.ad.nmsu.edu> Because SO many have contributed and since I'm having trouble working and laughing at the same time, here's a compiled list so EVERYONE can laugh! Keep 'em coming - the more the better. I'll keep collecting into next week, when I'll have another subject on which we can all expound. And then we'll get serious again. *"I know it's been a long week. Why don't all of you go home at noon and I'll take care of whatever comes in." *"I just learned grossing from watching CSI Miami." *"I don't need to see the slide; I'll take your word for it." *"I know it's STAT, but you still need your lunch." *"I don't want my slides until noon." *"No problem, I'll wait until the mounting medium has set before I look at the slide under oil immersion." *"I did immunostaining once, worked for me every time!" *"There is really no need for a tech to work Saturdays any more. We'll just have someone on call." *"Yes, I think it is a great idea that we close the lab for 2 weeks in December." *"That's OK, go ahead and leave. I'll clean up the gross area and start the VIP." *"Bubbles make slide look pretty and fun to look at." *"I just need one recut." *"I will always gross at 3mm or less just to help you out from now on, especially fatty specimens. I'll even teach the residents to gross smaller." *"I would really like to understand what you do, it looks complex. Perhaps I can watch and learn something." *"You work so hard, and I appreciate it so much! Why don't you sleep in on Monday? I'll cut my own slides." *"Don't worry about it - I'll clean the grossing area myself." *"I want one patient case per folder (20 slides/folder) even if there's only one slide on the case." *"Here, let me close that lid to the cryostat. You know...if you leave it open all the time, it really frosts up." *"Would you please show me how you would like breast tissue grossed in... to make your life easier?" *"I'm sorry, I didn't realize you can't embed a 1 cm thick piece of tissue." *"Please recut the slide thicker, it's too thin." *"Our pathologist left a note that asked us to do a 'Helicopter stain' on a gastric specimen, so my tech at the time asked him if he wanted us to do that at the airport." .... And my personal favorite so far........... **"Can you hold this cigarette for me? I need two hands for this." Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 From JMcCormick <@t> schosp.org Fri Nov 10 13:29:30 2006 From: JMcCormick <@t> schosp.org (McCormick, James) Date: Fri Nov 10 13:29:35 2006 Subject: [Histonet] Friday Fun In-Reply-To: <000601c704fd$62a9a330$7701a80a@Ford> Message-ID: <913FAC2B773C19488E26AE6572180FA5082DBDA4@exch01.schosp.org> Ford, Our list was the same but we had a "mono" processor and for special tissues, dip and dunk by hand. Regards, J.B.McCormick, M.D. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ford Royer Sent: Friday, November 10, 2006 1:21 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Friday Fun OH MY! Now you are really bringing back the memories of the old days. Our actual "End of Day" duty list in the late 60's, posted on the bulletin board, read something like this... 1. Remove and store microtome knifes. 2. Clean all areas of paraffin shavings. 3. Turn off water baths and embedding center. 4. Replace lids on stain and reagent containers. 5. Empty all ASH TRAYS. (!) 6. etc. 7. etc. Etc... Each bench had its own ash tray. Can you believe it?? The only place we were NOT allowed to smoke was in the room with the Technicon Duo processor. Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Friday, November 10, 2006 10:38 AM To: Edwards, R.E.; Pamela Marcum; Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Friday Fun as ashes were flicked into the trash can full of paraffin scraps and xylene soaked gauze..... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Edwards, R.E. Sent: Friday, November 10, 2006 11:36 AM To: Edwards, R.E.; Pamela Marcum; Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Friday Fun and heard in the good old days...."Can you hold the cigarette for me I need two hands for this" -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Pamela Marcum Sent: 10 November 2006 15:19 To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Friday Fun At 08:05 AM 11/10/2006, Breeden, Sara wrote: >Okay, here's the deal. Tell me, concisely, what would be the most >unlikely thing a histologist would hear from a pathologist? For >example, "No, please! Don't recut that broken slide - I can see just >fine through the cellophane tape". Send your innovative, imaginative, >and funny responses directly to me at sbreeden@nmda.nmsu.edu. This >could lead to a Very Funny Friday! "Start your engines!!" > > > >Sally Breeden, HT(ASCP) > >NM Dept. of Agriculture > >Veterinary Diagnostic Services > >PO Box 700 > >Albuquerque, NM 87106 > >505-841-2576 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet " I will always gross at 3mm or less just to help you out from now on, especially fatty specimens. I will even teach the residents to gross smaller!" Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From llewllew <@t> shaw.ca Fri Nov 10 13:33:35 2006 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Fri Nov 10 13:35:06 2006 Subject: [Histonet] Re:Bikes and Organ Donors Message-ID: <001f01c704ff$1db3c240$130e4246@yourlk4rlmsu> Originally BSA, Triumph and Norton were all independent companies producing competitive death trap speed bikes for 19 year teenagers. All were used for the Ton Up club, doing 100 mph on a British road. They were basically high efficiency engines with two wheels and inferior brakes with the person lying along the gas tank holding onto to sticks for steering (just a little exaggerated). All three bikes were inferior to the Velocette Venom Thruxton, which left all three standing. BMW was a middle aged rich guy's bike, and the envy of everyone else. The all went under because the Japanese bikes were faster. We called them whiz bangs because of the sound they made as they sped by. The calling of Japanese bikes being British was a sarcastic reference to them killing a revered industry. Oh to be young again. Bryan Lllewellyn ----- Original Message ----- From: "Pat Flannery" To: Sent: Friday, November 10, 2006 10:12 AM Subject: [Histonet] Re:Bikes and Organ Donors > While I'm asking, what's the difference between a BSA and a Norton or > Triumph, since they seem to be made by the same folks (according to > Wikipedia anyway)? Is it a Ford/Mercury/Lincoln type of thing or did one > "become" the other at some point? > > My apologies to the rest of the Histonetters for whom this is pretty > off-topic, but we've entered into an area near and dear to my heart (and > wallet). I'll return you to your regularly-scheduled discussions of > Histology after this post. Anyone willing to take this off-list is > welcome to continue, though. > > > -Pat Flannery > pjfnefro@duke.edu > > On Nov 10, 2006, at 12:54 PM, Pat Flannery wrote: > >> Answering my own question here, but I think I just figured out what a >> BSA is: Birmingham Small Arms merged with Norton International and the >> BSA motorcycle works was spun off from them. I guess I just never saw a >> BSA over here. Did they go OOB or are they still around (all the pics >> on the net seem to be old/classic bikes)? >> >> -Pat Flannery >> >> On Nov 10, 2006, at 12:44 PM, Pat Flannery wrote: >> >>> Thought I'd change the subject line since we've gone way past gloves >>> (does that mean the gloves are off?). >>> >>> Betsy, you've got me curious now and I'm going to show my ignorance >>> here, but what the heck's a BSA? BMW I know, but BSA sounds like >>> lyophilized cow serum, not a motorcycle. >>> >>> -Pat Flannery >>> >>> >>> On Nov 10, 2006, at 11:52 AM, Molinari, Betsy wrote: >>> >>>> Ouch! Besides our 2 Harleys I ride a Triumph Bonneville (antique >>>> restored) and we have a BSA we are rebuilding so am I exempt? >>>> >>>> Betsy Molinari HT (ASCP) >>>> Texas Heart Institute >>>> Cardiovascular Pathology >>>> 6770 Bertner Ave. >>>> Houston,TX 77030 >>>> 832-355-6524 >>>> 832-355-6812 (fax) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From froyer <@t> bitstream.net Fri Nov 10 13:51:54 2006 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Nov 10 13:52:32 2006 Subject: [Histonet] Re:Bikes and Organ Donors In-Reply-To: <001f01c704ff$1db3c240$130e4246@yourlk4rlmsu> Message-ID: <000b01c70501$ac817560$7701a80a@Ford> "Crotch Rockets" ... 0-60 mph in less than 5 seconds. "Oak Trees & Concrete Bridge Abutments" ... 60-0 in zero seconds. A statistic that they did not advertise to the macho teenagers that they were selling to. Ford -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan Llewellyn Sent: Friday, November 10, 2006 1:34 PM To: Histonet Subject: Re: [Histonet] Re:Bikes and Organ Donors Originally BSA, Triumph and Norton were all independent companies producing competitive death trap speed bikes for 19 year teenagers. All were used for the Ton Up club, doing 100 mph on a British road. They were basically high efficiency engines with two wheels and inferior brakes with the person lying along the gas tank holding onto to sticks for steering (just a little exaggerated). All three bikes were inferior to the Velocette Venom Thruxton, which left all three standing. BMW was a middle aged rich guy's bike, and the envy of everyone else. The all went under because the Japanese bikes were faster. We called them whiz bangs because of the sound they made as they sped by. The calling of Japanese bikes being British was a sarcastic reference to them killing a revered industry. Oh to be young again. Bryan Lllewellyn ----- Original Message ----- From: "Pat Flannery" To: Sent: Friday, November 10, 2006 10:12 AM Subject: [Histonet] Re:Bikes and Organ Donors > While I'm asking, what's the difference between a BSA and a Norton or > Triumph, since they seem to be made by the same folks (according to > Wikipedia anyway)? Is it a Ford/Mercury/Lincoln type of thing or did one > "become" the other at some point? > > My apologies to the rest of the Histonetters for whom this is pretty > off-topic, but we've entered into an area near and dear to my heart (and > wallet). I'll return you to your regularly-scheduled discussions of > Histology after this post. Anyone willing to take this off-list is > welcome to continue, though. > > > -Pat Flannery > pjfnefro@duke.edu > > On Nov 10, 2006, at 12:54 PM, Pat Flannery wrote: > >> Answering my own question here, but I think I just figured out what a >> BSA is: Birmingham Small Arms merged with Norton International and the >> BSA motorcycle works was spun off from them. I guess I just never saw a >> BSA over here. Did they go OOB or are they still around (all the pics >> on the net seem to be old/classic bikes)? >> >> -Pat Flannery >> >> On Nov 10, 2006, at 12:44 PM, Pat Flannery wrote: >> >>> Thought I'd change the subject line since we've gone way past gloves >>> (does that mean the gloves are off?). >>> >>> Betsy, you've got me curious now and I'm going to show my ignorance >>> here, but what the heck's a BSA? BMW I know, but BSA sounds like >>> lyophilized cow serum, not a motorcycle. >>> >>> -Pat Flannery >>> >>> >>> On Nov 10, 2006, at 11:52 AM, Molinari, Betsy wrote: >>> >>>> Ouch! Besides our 2 Harleys I ride a Triumph Bonneville (antique >>>> restored) and we have a BSA we are rebuilding so am I exempt? >>>> >>>> Betsy Molinari HT (ASCP) >>>> Texas Heart Institute >>>> Cardiovascular Pathology >>>> 6770 Bertner Ave. >>>> Houston,TX 77030 >>>> 832-355-6524 >>>> 832-355-6812 (fax) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DavidH <@t> marketlabinc.com Fri Nov 10 14:09:52 2006 From: DavidH <@t> marketlabinc.com (David Haagsma) Date: Fri Nov 10 14:10:03 2006 Subject: [Histonet] Re:Bikes and Organ Donors Message-ID: <19E3602A16438E48B51A4250CA04B5F695A669@exchange.marketlab.com> Ford, A little slow on the speed, my almost stock Honda 750cc does the 1/4 mile in just over 11 seconds: Results : Quarter Mile Acceleration: 11.402 seconds. Terminal speed: 129.6290 mph. - not when I crash - just the Quartermile mark. Dave Haagsma MT(ASCP) MarketLab Inc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ford Royer Sent: Friday, November 10, 2006 2:52 PM To: 'Histonet' Subject: RE: [Histonet] Re:Bikes and Organ Donors "Crotch Rockets" ... 0-60 mph in less than 5 seconds. "Oak Trees & Concrete Bridge Abutments" ... 60-0 in zero seconds. A statistic that they did not advertise to the macho teenagers that they were selling to. Ford -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan Llewellyn Sent: Friday, November 10, 2006 1:34 PM To: Histonet Subject: Re: [Histonet] Re:Bikes and Organ Donors Originally BSA, Triumph and Norton were all independent companies producing competitive death trap speed bikes for 19 year teenagers. All were used for the Ton Up club, doing 100 mph on a British road. They were basically high efficiency engines with two wheels and inferior brakes with the person lying along the gas tank holding onto to sticks for steering (just a little exaggerated). All three bikes were inferior to the Velocette Venom Thruxton, which left all three standing. BMW was a middle aged rich guy's bike, and the envy of everyone else. The all went under because the Japanese bikes were faster. We called them whiz bangs because of the sound they made as they sped by. The calling of Japanese bikes being British was a sarcastic reference to them killing a revered industry. Oh to be young again. Bryan Lllewellyn ----- Original Message ----- From: "Pat Flannery" To: Sent: Friday, November 10, 2006 10:12 AM Subject: [Histonet] Re:Bikes and Organ Donors > While I'm asking, what's the difference between a BSA and a Norton or > Triumph, since they seem to be made by the same folks (according to > Wikipedia anyway)? Is it a Ford/Mercury/Lincoln type of thing or did one > "become" the other at some point? > > My apologies to the rest of the Histonetters for whom this is pretty > off-topic, but we've entered into an area near and dear to my heart (and > wallet). I'll return you to your regularly-scheduled discussions of > Histology after this post. Anyone willing to take this off-list is > welcome to continue, though. > > > -Pat Flannery > pjfnefro@duke.edu > > On Nov 10, 2006, at 12:54 PM, Pat Flannery wrote: > >> Answering my own question here, but I think I just figured out what a >> BSA is: Birmingham Small Arms merged with Norton International and the >> BSA motorcycle works was spun off from them. I guess I just never saw a >> BSA over here. Did they go OOB or are they still around (all the pics >> on the net seem to be old/classic bikes)? >> >> -Pat Flannery >> >> On Nov 10, 2006, at 12:44 PM, Pat Flannery wrote: >> >>> Thought I'd change the subject line since we've gone way past gloves >>> (does that mean the gloves are off?). >>> >>> Betsy, you've got me curious now and I'm going to show my ignorance >>> here, but what the heck's a BSA? BMW I know, but BSA sounds like >>> lyophilized cow serum, not a motorcycle. >>> >>> -Pat Flannery >>> >>> >>> On Nov 10, 2006, at 11:52 AM, Molinari, Betsy wrote: >>> >>>> Ouch! Besides our 2 Harleys I ride a Triumph Bonneville (antique >>>> restored) and we have a BSA we are rebuilding so am I exempt? >>>> >>>> Betsy Molinari HT (ASCP) >>>> Texas Heart Institute >>>> Cardiovascular Pathology >>>> 6770 Bertner Ave. >>>> Houston,TX 77030 >>>> 832-355-6524 >>>> 832-355-6812 (fax) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMcCormick <@t> schosp.org Fri Nov 10 14:20:31 2006 From: JMcCormick <@t> schosp.org (McCormick, James) Date: Fri Nov 10 14:20:37 2006 Subject: [Histonet] Re:Bikes and Organ Donors In-Reply-To: <19E3602A16438E48B51A4250CA04B5F695A669@exchange.marketlab.com> Message-ID: <913FAC2B773C19488E26AE6572180FA5082DBDAB@exch01.schosp.org> Dave, Never done that, never been there....... BUT, I've seen a lot of Organs Donated that were surplus to the owners in their final RUSH of excitement! Good Luck ! J.B.McCormick, M.D. Pathologist -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Haagsma Sent: Friday, November 10, 2006 2:10 PM To: Ford Royer; Histonet Subject: RE: [Histonet] Re:Bikes and Organ Donors Ford, A little slow on the speed, my almost stock Honda 750cc does the 1/4 mile in just over 11 seconds: Results : Quarter Mile Acceleration: 11.402 seconds. Terminal speed: 129.6290 mph. - not when I crash - just the Quartermile mark. Dave Haagsma MT(ASCP) MarketLab Inc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ford Royer Sent: Friday, November 10, 2006 2:52 PM To: 'Histonet' Subject: RE: [Histonet] Re:Bikes and Organ Donors "Crotch Rockets" ... 0-60 mph in less than 5 seconds. "Oak Trees & Concrete Bridge Abutments" ... 60-0 in zero seconds. A statistic that they did not advertise to the macho teenagers that they were selling to. Ford -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan Llewellyn Sent: Friday, November 10, 2006 1:34 PM To: Histonet Subject: Re: [Histonet] Re:Bikes and Organ Donors Originally BSA, Triumph and Norton were all independent companies producing competitive death trap speed bikes for 19 year teenagers. All were used for the Ton Up club, doing 100 mph on a British road. They were basically high efficiency engines with two wheels and inferior brakes with the person lying along the gas tank holding onto to sticks for steering (just a little exaggerated). All three bikes were inferior to the Velocette Venom Thruxton, which left all three standing. BMW was a middle aged rich guy's bike, and the envy of everyone else. The all went under because the Japanese bikes were faster. We called them whiz bangs because of the sound they made as they sped by. The calling of Japanese bikes being British was a sarcastic reference to them killing a revered industry. Oh to be young again. Bryan Lllewellyn ----- Original Message ----- From: "Pat Flannery" To: Sent: Friday, November 10, 2006 10:12 AM Subject: [Histonet] Re:Bikes and Organ Donors > While I'm asking, what's the difference between a BSA and a Norton or > Triumph, since they seem to be made by the same folks (according to > Wikipedia anyway)? Is it a Ford/Mercury/Lincoln type of thing or did one > "become" the other at some point? > > My apologies to the rest of the Histonetters for whom this is pretty > off-topic, but we've entered into an area near and dear to my heart (and > wallet). I'll return you to your regularly-scheduled discussions of > Histology after this post. Anyone willing to take this off-list is > welcome to continue, though. > > > -Pat Flannery > pjfnefro@duke.edu > > On Nov 10, 2006, at 12:54 PM, Pat Flannery wrote: > >> Answering my own question here, but I think I just figured out what a >> BSA is: Birmingham Small Arms merged with Norton International and the >> BSA motorcycle works was spun off from them. I guess I just never saw a >> BSA over here. Did they go OOB or are they still around (all the pics >> on the net seem to be old/classic bikes)? >> >> -Pat Flannery >> >> On Nov 10, 2006, at 12:44 PM, Pat Flannery wrote: >> >>> Thought I'd change the subject line since we've gone way past gloves >>> (does that mean the gloves are off?). >>> >>> Betsy, you've got me curious now and I'm going to show my ignorance >>> here, but what the heck's a BSA? BMW I know, but BSA sounds like >>> lyophilized cow serum, not a motorcycle. >>> >>> -Pat Flannery >>> >>> >>> On Nov 10, 2006, at 11:52 AM, Molinari, Betsy wrote: >>> >>>> Ouch! Besides our 2 Harleys I ride a Triumph Bonneville (antique >>>> restored) and we have a BSA we are rebuilding so am I exempt? >>>> >>>> Betsy Molinari HT (ASCP) >>>> Texas Heart Institute >>>> Cardiovascular Pathology >>>> 6770 Bertner Ave. >>>> Houston,TX 77030 >>>> 832-355-6524 >>>> 832-355-6812 (fax) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From micro <@t> superlink.net Fri Nov 10 14:25:50 2006 From: micro <@t> superlink.net (Markus F. Meyenhofer) Date: Fri Nov 10 14:26:03 2006 Subject: [Histonet] Re:Bikes and Organ Donors References: <19E3602A16438E48B51A4250CA04B5F695A669@exchange.marketlab.com> Message-ID: <0cb501c70506$6a479fd0$93893cd1@DJ4VDH31> Bikes are nice but they all should come with a free coffin or wheelchair! ----- Original Message ----- From: "David Haagsma" To: "Ford Royer" ; "Histonet" Sent: Friday, November 10, 2006 3:09 PM Subject: RE: [Histonet] Re:Bikes and Organ Donors Ford, A little slow on the speed, my almost stock Honda 750cc does the 1/4 mile in just over 11 seconds: Results : Quarter Mile Acceleration: 11.402 seconds. Terminal speed: 129.6290 mph. - not when I crash - just the Quartermile mark. Dave Haagsma MT(ASCP) MarketLab Inc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ford Royer Sent: Friday, November 10, 2006 2:52 PM To: 'Histonet' Subject: RE: [Histonet] Re:Bikes and Organ Donors "Crotch Rockets" ... 0-60 mph in less than 5 seconds. "Oak Trees & Concrete Bridge Abutments" ... 60-0 in zero seconds. A statistic that they did not advertise to the macho teenagers that they were selling to. Ford -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan Llewellyn Sent: Friday, November 10, 2006 1:34 PM To: Histonet Subject: Re: [Histonet] Re:Bikes and Organ Donors Originally BSA, Triumph and Norton were all independent companies producing competitive death trap speed bikes for 19 year teenagers. All were used for the Ton Up club, doing 100 mph on a British road. They were basically high efficiency engines with two wheels and inferior brakes with the person lying along the gas tank holding onto to sticks for steering (just a little exaggerated). All three bikes were inferior to the Velocette Venom Thruxton, which left all three standing. BMW was a middle aged rich guy's bike, and the envy of everyone else. The all went under because the Japanese bikes were faster. We called them whiz bangs because of the sound they made as they sped by. The calling of Japanese bikes being British was a sarcastic reference to them killing a revered industry. Oh to be young again. Bryan Lllewellyn ----- Original Message ----- From: "Pat Flannery" To: Sent: Friday, November 10, 2006 10:12 AM Subject: [Histonet] Re:Bikes and Organ Donors > While I'm asking, what's the difference between a BSA and a Norton or > Triumph, since they seem to be made by the same folks (according to > Wikipedia anyway)? Is it a Ford/Mercury/Lincoln type of thing or did one > "become" the other at some point? > > My apologies to the rest of the Histonetters for whom this is pretty > off-topic, but we've entered into an area near and dear to my heart (and > wallet). I'll return you to your regularly-scheduled discussions of > Histology after this post. Anyone willing to take this off-list is > welcome to continue, though. > > > -Pat Flannery > pjfnefro@duke.edu > > On Nov 10, 2006, at 12:54 PM, Pat Flannery wrote: > >> Answering my own question here, but I think I just figured out what a >> BSA is: Birmingham Small Arms merged with Norton International and the >> BSA motorcycle works was spun off from them. I guess I just never saw a >> BSA over here. Did they go OOB or are they still around (all the pics >> on the net seem to be old/classic bikes)? >> >> -Pat Flannery >> >> On Nov 10, 2006, at 12:44 PM, Pat Flannery wrote: >> >>> Thought I'd change the subject line since we've gone way past gloves >>> (does that mean the gloves are off?). >>> >>> Betsy, you've got me curious now and I'm going to show my ignorance >>> here, but what the heck's a BSA? BMW I know, but BSA sounds like >>> lyophilized cow serum, not a motorcycle. >>> >>> -Pat Flannery >>> >>> >>> On Nov 10, 2006, at 11:52 AM, Molinari, Betsy wrote: >>> >>>> Ouch! Besides our 2 Harleys I ride a Triumph Bonneville (antique >>>> restored) and we have a BSA we are rebuilding so am I exempt? >>>> >>>> Betsy Molinari HT (ASCP) >>>> Texas Heart Institute >>>> Cardiovascular Pathology >>>> 6770 Bertner Ave. >>>> Houston,TX 77030 >>>> 832-355-6524 >>>> 832-355-6812 (fax) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Fri Nov 10 14:28:25 2006 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Fri Nov 10 14:28:56 2006 Subject: [Histonet] Zen and the art of Histo excellence maintainance.... Message-ID: <006a01c70506$c66fd930$0201a8c0@carlba65530bda> By Pirsig....to paraphrase ;-) Reminds me of all things Histo: just having read all the Motorbikin' stuff. Loads of continual fine-tuning; some machines work better than others ;-) Let's just make sure we have petrol in our tanks...... Carl NB: every time I use BSA in my immunobuffers, I fondly remember the OIL-Leaking BSA/Triumphs/ From tp2 <@t> medicine.wisc.edu Fri Nov 10 14:39:38 2006 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Fri Nov 10 14:40:23 2006 Subject: [Histonet] Re:Bikes and Organ Donors Message-ID: <45548F2A020000DF00002B05@gwmail.medicine.wisc.edu> Well... I ride a motorcycle and I'm an organ donor. Every close call that I've ever had has been due to some idiot in a car, truck, suv, or bus not being away of the traffic around them. I suspect that these are the same people that complain about motorcycles just coming out of nowhere. Riding a bike makes you a better driver in your car too. I see things that I never used see and can anticipate who's about to do something stupid on the road like never before. As for the idiot who likes to race his crotchrocket up my two block long street at 60 mph; I'd like to stretch a piece of cable across the road at about 4 feet high so that somebody with half a brain can have his organs. Tom >>> "Markus F. Meyenhofer" 11/10/06 2:25 PM >>> Bikes are nice but they all should come with a free coffin or wheelchair! ----- Original Message ----- From: "David Haagsma" To: "Ford Royer" ; "Histonet" Sent: Friday, November 10, 2006 3:09 PM Subject: RE: [Histonet] Re:Bikes and Organ Donors Ford, A little slow on the speed, my almost stock Honda 750cc does the 1/4 mile in just over 11 seconds: Results : Quarter Mile Acceleration: 11.402 seconds. Terminal speed: 129.6290 mph. - not when I crash - just the Quartermile mark. Dave Haagsma MT(ASCP) MarketLab Inc. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ford Royer Sent: Friday, November 10, 2006 2:52 PM To: 'Histonet' Subject: RE: [Histonet] Re:Bikes and Organ Donors "Crotch Rockets" ... 0-60 mph in less than 5 seconds. "Oak Trees & Concrete Bridge Abutments" ... 60-0 in zero seconds. A statistic that they did not advertise to the macho teenagers that they were selling to. Ford -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bryan Llewellyn Sent: Friday, November 10, 2006 1:34 PM To: Histonet Subject: Re: [Histonet] Re:Bikes and Organ Donors Originally BSA, Triumph and Norton were all independent companies producing competitive death trap speed bikes for 19 year teenagers. All were used for the Ton Up club, doing 100 mph on a British road. They were basically high efficiency engines with two wheels and inferior brakes with the person lying along the gas tank holding onto to sticks for steering (just a little exaggerated). All three bikes were inferior to the Velocette Venom Thruxton, which left all three standing. BMW was a middle aged rich guy's bike, and the envy of everyone else. The all went under because the Japanese bikes were faster. We called them whiz bangs because of the sound they made as they sped by. The calling of Japanese bikes being British was a sarcastic reference to them killing a revered industry. Oh to be young again. Bryan Lllewellyn ----- Original Message ----- From: "Pat Flannery" To: Sent: Friday, November 10, 2006 10:12 AM Subject: [Histonet] Re:Bikes and Organ Donors > While I'm asking, what's the difference between a BSA and a Norton or > Triumph, since they seem to be made by the same folks (according to > Wikipedia anyway)? Is it a Ford/Mercury/Lincoln type of thing or did one > "become" the other at some point? > > My apologies to the rest of the Histonetters for whom this is pretty > off-topic, but we've entered into an area near and dear to my heart (and > wallet). I'll return you to your regularly-scheduled discussions of > Histology after this post. Anyone willing to take this off-list is > welcome to continue, though. > > > -Pat Flannery > pjfnefro@duke.edu > > On Nov 10, 2006, at 12:54 PM, Pat Flannery wrote: > >> Answering my own question here, but I think I just figured out what a >> BSA is: Birmingham Small Arms merged with Norton International and the >> BSA motorcycle works was spun off from them. I guess I just never saw a >> BSA over here. Did they go OOB or are they still around (all the pics >> on the net seem to be old/classic bikes)? >> >> -Pat Flannery >> >> On Nov 10, 2006, at 12:44 PM, Pat Flannery wrote: >> >>> Thought I'd change the subject line since we've gone way past gloves >>> (does that mean the gloves are off?). >>> >>> Betsy, you've got me curious now and I'm going to show my ignorance >>> here, but what the heck's a BSA? BMW I know, but BSA sounds like >>> lyophilized cow serum, not a motorcycle. >>> >>> -Pat Flannery >>> >>> >>> On Nov 10, 2006, at 11:52 AM, Molinari, Betsy wrote: >>> >>>> Ouch! Besides our 2 Harleys I ride a Triumph Bonneville (antique >>>> restored) and we have a BSA we are rebuilding so am I exempt? >>>> >>>> Betsy Molinari HT (ASCP) >>>> Texas Heart Institute >>>> Cardiovascular Pathology >>>> 6770 Bertner Ave. >>>> Houston,TX 77030 >>>> 832-355-6524 >>>> 832-355-6812 (fax) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Nov 10 14:41:09 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Nov 10 14:41:16 2006 Subject: [Histonet] Mystery Fixative In-Reply-To: Message-ID: <20061110204109.56915.qmail@web61219.mail.yahoo.com> Glen: Perhaps she is referring to "Jores's fixative". There are 2 formulas, one from 1896 and the other from 1913 The original one (1896) is as follows: Water -- 100 mL + 40% formaldehyde--10 mL + spdium sulfate -- 2g + magnesium sulfate -- 2g + sodium chloride -- 1 g Hope this will help you! Ren? J. "Dawson, Glen" wrote: All, Our AP supervisor is looking for an older fixative that she used to use to revitalize staining on tissue that had been sitting in formalin for months. She seems to think it is called "Yori's, Youri's or Jori's" fixative and they used to make it on-site back in the day but the recipe has long since been lost. Any Help Would be Greatly Appreciated, Glen Dawson IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Cheap Talk? Check out Yahoo! Messenger's low PC-to-Phone call rates. From SecrestK <@t> wvuh.com Fri Nov 10 15:35:08 2006 From: SecrestK <@t> wvuh.com (Secrest, Kimberly) Date: Fri Nov 10 15:35:31 2006 Subject: [Histonet] Formalin rotation on a Thermo Excelsior Message-ID: <7708E0A8E46BDC40B23113F97FB0FB2301B9C3E3@nt-exchange1.wvuh.wvuhs.com> I have two Thermo electron processors that are set up to have the 10% formalin changed every 4th run. We run about 100-125 blocks on each processor every night with one hour in Fix1 and another hour in Fix2. I was just putting my feelers out there to get input from other labs that may have the same equipment and what their rotation schedule is. Thanks Kimberly Secrest HTL, QIHC Histology Technical Specialist West Virginia University Hospital From pjfnefro <@t> duke.edu Fri Nov 10 15:35:47 2006 From: pjfnefro <@t> duke.edu (Pat Flannery) Date: Fri Nov 10 15:35:58 2006 Subject: [Histonet] Re:Bikes and Organ Donors In-Reply-To: <0cb501c70506$6a479fd0$93893cd1@DJ4VDH31> References: <19E3602A16438E48B51A4250CA04B5F695A669@exchange.marketlab.com> <0cb501c70506$6a479fd0$93893cd1@DJ4VDH31> Message-ID: <4554F0B3.1020909@duke.edu> Markus F. Meyenhofer wrote: > Bikes are nice but they all should come with a free coffin or wheelchair! Kind of harsh, don't you think, Markus? All those negative waves directed at us poor bikers? -Pat From debbiekeith <@t> cox.net Fri Nov 10 15:39:41 2006 From: debbiekeith <@t> cox.net (Debbie Keith) Date: Fri Nov 10 15:39:50 2006 Subject: [Histonet] Zen and the art of Histo excellence maintainance.... In-Reply-To: <006a01c70506$c66fd930$0201a8c0@carlba65530bda> Message-ID: <5.2.0.9.0.20061110143821.02f0be98@pop.central.cox.net> At 08:28 PM 11/10/2006 +0000, you wrote: >By Pirsig....to paraphrase ;-) >Reminds me of all things Histo: just having read all the Motorbikin' stuff. >Loads of continual fine-tuning; some machines work better than others ;-) >Let's just make sure we have petrol in our tanks...... >Carl >NB: every time I use BSA in my immunobuffers, I fondly remember the >OIL-Leaking BSA/Triumphs/ > > >_ triumphs no longer leak oil.... (how offensive)... but they do install faulty bits in the fuel line, so occasionally they will spew gas on the garage floor (before the 1000 mile tune-up). don't ask me how i know. ;) deb -- No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.1.409 / Virus Database: 268.14.2/528 - Release Date: 11/10/2006 From jimr0712 <@t> comcast.net Fri Nov 10 15:45:12 2006 From: jimr0712 <@t> comcast.net (jimr0712@comcast.net) Date: Fri Nov 10 15:49:15 2006 Subject: [Histonet] Re: Histonet Digest, Vol 36, Issue 15 Message-ID: <111020062145.27343.4554F2E800054CE900006ACF2200737478CDCEC9CF9D030706@comcast.net> This message has been processed by Symantec's AntiVirus Technology. Unknown00000000.data was not scanned for viruses because too many nested levels of files were found. For more information on antivirus tips and technology, visit http://ses.symantec.com/ From liz <@t> premierlab.com Fri Nov 10 16:52:44 2006 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Nov 10 16:44:33 2006 Subject: [Histonet] alpha gal or gsib4 lectin staining in porcine tissue Message-ID: <000a01c7051a$ef465140$0300a8c0@domain.Premier> Hello everyone Does anyone have any experience in staining for alpha gal in FFPE porcine tissues. There is a protocol on IHC world and we have run some initial tests on this, and we seem to be getting staining in the endothelial cells, serum and a few other positive cells here and there. Initially the client was thinking that we should have more staining. We are using Axxora's antibody and have ran dilutions from 1:100 to 1:800 (pH 9 retreival) and they all look about the same and we are really not getting any background staining so we were concerned if our sensitivity was o.k. I've done numerous internet searches and have not come up with much and also a bit a searching on the gsib4 staining which you can get from vector labs catalog FT1201, the description for this antibody tells me that it stains endothelial cells, so I believe that I'm on the right tract and that my staining pattern is correct, but any help would be appreciated. Have a great weekend Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 From dfinkelstein <@t> mhri.edu.au Fri Nov 10 17:58:44 2006 From: dfinkelstein <@t> mhri.edu.au (David Finkelstein) Date: Fri Nov 10 17:59:42 2006 Subject: [Histonet] FW: Something new - -Something Old. Message-ID: <001801c70524$28000540$0200a8c0@davidfink> From: David Finkelstein To: 'Jackie.O'Connor@abbott.com' Subject: Something new - -Something Old. Hi Jackie "Can someone advise me the best way to preserve (Liguid nitrogen, OCT, fixed?) and visualize tissues from animals which have been administered (in vivo) a fluorescent probe" Strange question. Your tissue does not appear to be fixed. For animal experiments the best is to paraformaldhye perfuse, then cryoprotect with sucrose, snap freeze the blocks, nooct, Cut and store the sections at -80oc. Please find attached a references: 1. Kha, H.T., D.I. Finkelstein, D.V. Pow, A.J. Lawrence, and M.K. Horne, Study of projections from the entopeduncular nucleus to the thalamus of the rat. Journal of Comparative Neurology, 2000. 426(3): p. 366-77. 2. Kha, H.T., D.I. Finkelstein, D. Tomas, J. Drago, D.V. Pow, and M.K. Horne, Projections from the substantia nigra pars reticulata to the motor thalamus of the rat: single axon reconstructions and immunohistochemical study. Journal of Comparative Neurology, 2001. 440(1): p. 20-30. Yours, David , Assoc. Professor David Finkelstein, The Mental Health Research Institute of Victoria, 155 Oak Street, Parkville, Victoria 3052 AUSTRALIA Mobile: 0409171227 Tel: +61 (03) 9388 1633 Fax: +61 (03) 9387 5061 dfinkelstein@mhri.edu.au Message: 1 Date: Fri, 10 Nov 2006 11:54:23 -0600 From: "Jackie M O'Connor" < > Subject: Re: [Histonet] Something new - -I'm waiting To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" OK - so I read my own question and it doesn't make enough sense. Maybe I can elaborate a bit - Is it possible to administer a fluorescent probe in vivo (Like GFP) visualize that probe in vivo, then harvest tissues and be able to see that probe in frozen sections using a fluorescent scope? "Jackie M O'Connor" Sent by: histonet-bounces@lists.utsouthwestern.edu 11/10/2006 09:49 AM To histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu cc Subject [Histonet] Something new Can someone advise me the best way to preserve (Liguid nitrogen, OCT, fixed?) and visualize tissues from animals which have been administered (in vivo) a fluorescent probe? Thanks, Jackie O' on a crummy Friday morning fighting fires again as usual. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From debbiekeith <@t> cox.net Fri Nov 10 19:06:30 2006 From: debbiekeith <@t> cox.net (Debbie Keith) Date: Fri Nov 10 19:06:39 2006 Subject: [Histonet] Mohs tech salaries... based on productivity.... Message-ID: <5.2.0.9.0.20061110180444.02f06308@pop.central.cox.net> hi all! i have heard about some Mohs techs (in private practices) that are setting their salaries on case count rather than by hour. Is anyone doing that here? thanks in advance... deb -- No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.1.409 / Virus Database: 268.14.2/528 - Release Date: 11/10/2006 From RSRICHMOND <@t> aol.com Sat Nov 11 12:39:28 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Sat Nov 11 12:39:38 2006 Subject: [Histonet] Re: Helicobacter controls Message-ID: Lee and Peggy Wenk asked about Helicobacter stain controls. Every lab I've ever worked in just used its positive slides as controls, and I've found that to be satisfactory. The toluidine blue (e.g. Diff-Quik) stains don't really need controls. In labs where the histotechs never look at a slide, it's common to find that the control isn't positive - I sign these out as "with a suitable control" for regulatory purposes. - The IHC of course requires a control. Rather rarely these days, you get a gastrectomy specimen that's positive, and one of these would give you controls for the rest of your life - look for them. Helicobacter heilmanii (AKA Gastrospirillum hominis) has the same IHC reactivity as H. pylori - there's literature about this - it's more common in Japan than in the USA. Bob Richmond Samurai Pathologist Knoxville TN From rjbuesa <@t> yahoo.com Sat Nov 11 13:42:52 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Nov 11 13:42:58 2006 Subject: [Histonet] Re: Helicobacter controls In-Reply-To: Message-ID: <20061111194252.26896.qmail@web61219.mail.yahoo.com> I used modified Steiner for H. pyloris and this is an unspecific silver stain, it will stain bacteria so, after much arguments with the pathologists, I was allowed to use the organisms in appendix sections as + controls, because if those bacteria were stained, so would H. pyloris. That made my work much easy! Ren? J. RSRICHMOND@aol.com wrote: Lee and Peggy Wenk asked about Helicobacter stain controls. Every lab I've ever worked in just used its positive slides as controls, and I've found that to be satisfactory. The toluidine blue (e.g. Diff-Quik) stains don't really need controls. In labs where the histotechs never look at a slide, it's common to find that the control isn't positive - I sign these out as "with a suitable control" for regulatory purposes. - The IHC of course requires a control. Rather rarely these days, you get a gastrectomy specimen that's positive, and one of these would give you controls for the rest of your life - look for them. Helicobacter heilmanii (AKA Gastrospirillum hominis) has the same IHC reactivity as H. pylori - there's literature about this - it's more common in Japan than in the USA. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Access over 1 million songs - Yahoo! Music Unlimited. From gu.lang <@t> gmx.at Sat Nov 11 14:17:30 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Nov 11 14:16:43 2006 Subject: [Histonet] fixation-density Message-ID: <000301c705ce$6a63b670$c812a8c0@dielangs.at> Hi all, I'm still dealing with trichrome staining. There is the theory of diffusion-ratio of smaller and bigger dyes. I have a problem of understanding: Why is the fixed cytoplasma, formerly filled with more or less soluble proteins beside the cytokeratins, more dense than the fibrillar and twisted collagenfibers. Are the spaces between the helices bigger than the mesh of the crosslinked proteins? There is much water in a cell (yes?) and the proteins are sticked together through fixation. Can someone bring light into my darkness? Gudrun Lang Histo-BMA Linz, Austria From llewllew <@t> shaw.ca Sat Nov 11 16:13:03 2006 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Sat Nov 11 16:15:40 2006 Subject: [Histonet] fixation-density References: <000301c705ce$6a63b670$c812a8c0@dielangs.at> Message-ID: <000c01c705de$8f035f70$130e4246@yourlk4rlmsu> First, this is a thoery that explains the observable results, but I am not sure it has ever been proven formally. The word "dense" in this context can heve two different meanings, and it is possible that your difficulty is related to that. Meaning 1: (Chemical) More foci at which the dye can attach in an amount of stainable material, i.e. more strongly acidophil. Meaning 2: (Physical) More stainable material contained in a given space. In addition there is the question of "permeability", i.e. whether there is a barrier of some kind (lipid, cell membrane). With meaning 1, the dye will take longer to be replaced because there is more of it in an equal amount of material. In meaning 2, the dye has less accessiblity but when it does stain the polyacids will have the same accessibility problem or may be too large to gain access to remove it. Sometimes it is so physically dense it does not stain at all. A given structure could also be "dense" in both meanings. Fibrin is generally considered dense in the chemical meaning and tendon in the physical. Cytoplasm is thought of as less chemically dense than fibrin. It is covered in some detail on StainsFile at the URLs below. Follow all the links in the second one, about trichrome staining. It may help to use the BabelFish translator below the adverts on the right hand side. The translation is not perfect, but reading the translation and the English may help. http://stainsfile.info/StainsFile/theory/massact.htm http://stainsfile.info/StainsFile/theory/tri_gen.htm Bryan Llewellyn ----- Original Message ----- From: "Gudrun Lang" To: "Histonetliste (Histonetliste)" Sent: Saturday, November 11, 2006 12:17 PM Subject: [Histonet] fixation-density > Hi all, > > I'm still dealing with trichrome staining. There is the theory of > diffusion-ratio of smaller and bigger dyes. I have a problem of > understanding: > > Why is the fixed cytoplasma, formerly filled with more or less soluble > proteins beside the cytokeratins, more dense than the fibrillar and > twisted > collagenfibers. Are the spaces between the helices bigger than the mesh of > the crosslinked proteins? There is much water in a cell (yes?) and the > proteins are sticked together through fixation. > > Can someone bring light into my darkness? > > > > Gudrun Lang > > > > Histo-BMA > > Linz, Austria > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From beingmary53 <@t> sbcglobal.net Sun Nov 12 08:35:10 2006 From: beingmary53 <@t> sbcglobal.net (MARY JOHNSON) Date: Sun Nov 12 08:35:20 2006 Subject: [Histonet] special stains Message-ID: <20061112143510.25192.qmail@web81607.mail.mud.yahoo.com> Hi Breeden, We use the boxes that our Coverslips come, It same space and you can change them often. hope this help Mary johnson HT ( ASCP) From mikael.niku <@t> helsinki.fi Mon Nov 13 01:21:10 2006 From: mikael.niku <@t> helsinki.fi (Mikael Niku) Date: Mon Nov 13 01:21:24 2006 Subject: [Histonet] Autofluorescence In-Reply-To: References: Message-ID: <45581CE6.7050802@helsinki.fi> Hello! I tried the photobleaching method after reading the paper about it, but couldn't make it work. Briefly, I purchased fluorescent tubes with specific wavelengths and exposed paraffin sections to them up to 2-3 days or so, with absolutely no observable effect at all. Tried several wavelengths, no success. Perhaps I was doing something wrong, but I suspect the energy should be much higher (I think the authors actually recommended using an epifluorescence microscope with a low-power objective for best results; for large numbers of slides, this is obviously not feasible). I have got great results with 0.1% Sudan Black B (CI 26150) in 70% ethanol (for PFA-fixed, paraffin-embedded 4 um sections of various bovine tissues), applied for 15-30 min after the fluorescent secondary antibody. Note that the stuff takes time to dissolve. With best regards, Mikael Stephen Clark wrote: > My name is Stephen Clark and i work in the neuro lab at Eastern Illinois University. We use fluorescent immunohistochemistry to stain the Olfactory Epithelium of mice and have recently had a problem with autofluorescence of our tissues. They are fixed in 4% paraformaldehyde in PBS and i have already tried sodium borohydrate with little success. I have read about photobleaching using UV, neon, and lights of specific wavelengths (488 and 633nm). I am just wondering if anyone utilizes photobleaching via a light source and where it would be possible to purchase them. I am also wondering if the 18um sections might be a little too thick and whether that would have an increased affect on autofluorescence. Any tips would be appreciated. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- //////////////////////////////////////////////////////////// Mikael Niku URL: www.helsinki.fi/~mniku/ University of Helsinki Dept. Basic Veterinary Sciences - Mit?k? mielt? olen l?nsimaisesta sivistyksest?? Minusta se olisi erinomainen ajatus! - Gandhi //////////////////////////////////////////////////////////// From Kemlo.Rogerson <@t> waht.swest.nhs.uk Mon Nov 13 02:23:31 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Nov 13 02:22:40 2006 Subject: [Histonet] Bikes Message-ID: <86ADE4EB583CE64799A9924684A0FBBFAFC93E@wahtntex2.waht.swest.nhs.uk> Duh... That's my point. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Habits of thinking need not be forever. One of the most significant findings in psychology in the last twenty years is that individuals can choose the way they think. --Martin Seligman This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Mon Nov 13 02:28:00 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Nov 13 02:27:04 2006 Subject: [Histonet] Re: Cut resistant gloves Message-ID: <86ADE4EB583CE64799A9924684A0FBBFAFC93F@wahtntex2.waht.swest.nhs.uk> Agree (for a change). Harley's are big, ugly, sound like tractors, and invariably ridden by apes. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk Knew a girl like that. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Habits of thinking need not be forever. One of the most significant findings in psychology in the last twenty years is that individuals can choose the way they think. --Martin Seligman This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From jengirl1014 <@t> yahoo.com Mon Nov 13 06:56:31 2006 From: jengirl1014 <@t> yahoo.com (Jennifer Sipes) Date: Mon Nov 13 06:56:42 2006 Subject: [Histonet] Filipin staining Message-ID: <20061113125631.76854.qmail@web62414.mail.re1.yahoo.com> Does anyone have a protocol for this? It's to stain for cholesterol. Thanks for the help! Jen From pjfnefro <@t> duke.edu Mon Nov 13 07:34:45 2006 From: pjfnefro <@t> duke.edu (Pat Flannery) Date: Mon Nov 13 07:34:55 2006 Subject: [Histonet] Re: Cut resistant gloves In-Reply-To: <86ADE4EB583CE64799A9924684A0FBBFAFC93F@wahtntex2.waht.swest.nhs.uk> References: <86ADE4EB583CE64799A9924684A0FBBFAFC93F@wahtntex2.waht.swest.nhs.uk> Message-ID: <88D800F3-EE35-443E-9122-05BDC12DA478@duke.edu> Kemlo- Thanks for starting my week with a huge laugh! Didn't see that one coming. -Pat Flannery On Nov 13, 2006, at 3:28 AM, Kemlo Rogerson wrote: > Agree (for a change). > > Harley's are big, ugly, sound like tractors, and invariably ridden by > apes. > > > > Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant > Pathologist Rotherham General Hospital South Yorkshire England > > terry.marshall@rothgen.nhs.uk > > > > Knew a girl like that. > > > > Kemlo Rogerson > From liz <@t> premierlab.com Mon Nov 13 10:26:27 2006 From: liz <@t> premierlab.com (Liz Chlipala) Date: Mon Nov 13 10:18:12 2006 Subject: [Histonet] Filipin staining In-Reply-To: <20061113125631.76854.qmail@web62414.mail.re1.yahoo.com> Message-ID: <001c01c70740$783e5200$0300a8c0@domain.Premier> Jennifer I have a couple references that I'll e-mail you separately. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Sipes Sent: Monday, November 13, 2006 5:57 AM To: Histonet Subject: [Histonet] Filipin staining Does anyone have a protocol for this? It's to stain for cholesterol. Thanks for the help! Jen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1863 (20061113) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From NMargaryan <@t> childrensmemorial.org Mon Nov 13 11:37:18 2006 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Mon Nov 13 11:32:42 2006 Subject: [Histonet] Friday Fun Message-ID: <63B8B599DE283148B92E83C78B32C15D036810B8@cmhexbe2.childrensmemorial.org> Hi everybody, I am not a judge but I think this one is best one :) Naira:-) Message: 13 Date: Fri, 10 Nov 2006 16:35:58 -0000 From: "Edwards, R.E." Subject: RE: [Histonet] Friday Fun To: "Edwards, R.E." , "Pamela Marcum" , "Breeden, Sara" , Message-ID: Content-Type: text/plain; charset="us-ascii" and heard in the good old days...."Can you hold the cigarette for me I need two hands for this" From mwstarbu <@t> mdanderson.org Mon Nov 13 11:59:00 2006 From: mwstarbu <@t> mdanderson.org (mwstarbu@mdanderson.org) Date: Mon Nov 13 11:59:33 2006 Subject: [Histonet] New Bone by Von Kossa Van Giesen and UV light? Message-ID: Hello, I am hoping someone can help me with this. I'm trying to determine If I'm looking at new bone. I have performed a Von Kossa/Van Giesen stain on undecalcified calvaria sections. By using UV light with a TRITC filter, mineralized (black) areas become translucent or fluoresce. I'm not quite sure how to describe it. I have posted 3 pictures to demonstrate. no-uv.tif : Shows the calvaria under bright field with TRITC filter. You can see all the mineralized bone stained black. uv.tif: Shows the same image above but with UV light and the TRITC filter. I have drawn a green linr on the image to show where the mineralized bone extends when you are not using UV and the filter. halfshutter.tif: Shows the same image above but with the UV ligh at half shutter. the left half of the image shows the translucent area at the edge while the right side without UV shows where the mineralized region extends. If somone could please help me determine what I'm really looking at I would greatly appreciate it. Thank you in advance, Mike Starbuck From cmiller <@t> physlab.com Mon Nov 13 12:07:47 2006 From: cmiller <@t> physlab.com (Cheri Miller) Date: Mon Nov 13 12:08:03 2006 Subject: [Histonet] (no subject) Message-ID: <000001c7074e$a1e01810$db01a8c0@plab.local> Hi everyone! I am looking for a manufacturer or even a name of the small staining racks. Most use them for staining frozens or specials. The are metal with a long attached handle and they will hold only 5 or 6 slides? I cant seem to find them in any of our catalogs...Thanks, Cheri Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne From atouvr <@t> yahoo.com Mon Nov 13 05:26:55 2006 From: atouvr <@t> yahoo.com (annamaria touvra) Date: Mon Nov 13 12:48:23 2006 Subject: [Histonet] Help In-Reply-To: <86509.40529.qm@web30411.mail.mud.yahoo.com> Message-ID: <20061113112655.26512.qmail@web60723.mail.yahoo.com> Hello! I would appreciate if you could give me some advise on the Von Kossa silver method protocol on frozen sections of aorta. Could you please tell me how could I do the last part of dehydration (use of ethanol) and clearing (use of xylene) of the sections? I use for the dehydration 90% ethanol for 1 minute and 100% ethanol for 1 minute, and for the clearing 1 minute Xylene. I think I do not get good and acceptable results, while control samples stain with the same pattern. Thank you in advance, Anna-Maria ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Anna-Maria Touvra MSc Democritus University of Thrace Department of Physical Education and Sports Sciences Address: Kouloglou 4, Komotini 69100, GREECE Tel: 00306938511677, 00302531030343 Contact information in Finland Address: Haperontie 1B 31, 40640 JYV?SKYL?, FINLAND Tel: 00358509324688 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ --------------------------------- Access over 1 million songs - Yahoo! Music Unlimited. From lhotaks <@t> mcmaster.ca Mon Nov 13 12:48:52 2006 From: lhotaks <@t> mcmaster.ca (Sarka Lhotak) Date: Mon Nov 13 12:49:42 2006 Subject: [Histonet] Filipin staining Message-ID: Hi Jennifer, I've done some Filipin staining on cells and tissues. Filipin stains free cholesterol which can be found in membranes, i.e. plasma membrane and intracellular membranes, such as endoplasmic reticulum, membranes of vesicles et., and you can find free cholestrol also in extracellular crystals in the necrotic cores of lesions. Staining is done on frozen sections fixed with formalin or paraformaldehyde and rinsed with PBS (or cells treated similarly). You have to prepare the staining solution FRESH. I buy Filipin from Sigma, dissolve a little crystal of it in 5 microlitres of DMSO, then add 500 microlitres of PBS and use it for staining immediately. Stain for 1-2 hrs, rinse with PBS, then in distilled water and mount in Permafluor (Shandon). Observe in fluorescence microscope under UV light (same configuration as for DAPI or Hoechst). It bleaches quickly, you have to photograph it fast. Hope it helps. Let me know if you have more questions. Sarka Lhotak, PhD McMaster University, Hamilton, ON From orichman <@t> gmail.com Mon Nov 13 13:19:00 2006 From: orichman <@t> gmail.com (Omer Richman) Date: Mon Nov 13 13:19:10 2006 Subject: [Histonet] Cells Embedded in Collagen for Frozen Tissue Sectioning Message-ID: <5942d2e00611131119r68e9f7bcya110c88b4d5fbb5f@mail.gmail.com> Dear Histonet, My PI has been embedding cancer cells in collagen over the past several weeks. My job is to take the collagen bead (approx 1-1.5 cm in diameter), embed it in OCT and get frozen sections for H&E staining. Unfortunately, I have been having a very hard time finding the cells in the collagen in the OCT block because there is little to no contrast between the two. On top of the that, the collagen detaches itself from the OCT as it hits the blade leaving me with a square section of OCT that is missing a circle in the middle (presumably where the collagen and cells would be if I could see them). I have gone through several full specimens and have come away with only a slide or two where I managed to catch some cells. This sort of inconsistency is obviously not acceptable and my resources in the pathology department have come up dry. Does anyone have any experience with this sort of experiment? Is there any way to stain the collagen/cells in such a way that I could at least tell where the cells are in the OCT block so I'm not just blindly cutting away? Any help would be greatly appreciated. Thanks to everyone in advance. Sincerely, Omer Richman Research Technician State University of New York at Stony Brook Life Sciences Building Rm 004 Stony Brook, NY 11794 From ZHANGLI <@t> pathology.ufl.edu Mon Nov 13 13:30:32 2006 From: ZHANGLI <@t> pathology.ufl.edu (Li Zhang) Date: Mon Nov 13 13:31:20 2006 Subject: [Histonet] Please remove me from the histonet list. Message-ID: Please remove me from the histonet list. Thank you Li Zhang From gcallis <@t> montana.edu Mon Nov 13 13:45:12 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Nov 13 13:45:35 2006 Subject: [Histonet] Cells Embedded in Collagen for Frozen Tissue Sectioning In-Reply-To: <5942d2e00611131119r68e9f7bcya110c88b4d5fbb5f@mail.gmail.co m> References: <5942d2e00611131119r68e9f7bcya110c88b4d5fbb5f@mail.gmail.com> Message-ID: <6.0.0.22.1.20061113123703.01b3b698@gemini.msu.montana.edu> You do not need a collagen matrix to make a frozen cell block. Take 3 x 10 to the 7th ( higher power 7) cells suspended in PBS without any protein carrier. Spin down and wash 3 times with PBS, after the last spin, add approx 1 ml or less of OCT to the cell pellet, resuspend the cells. Snap freeze end of tube in liquid nitrogen and using a sharp rap on bottom of tube, dislodge the block, mount on a chuck and do frozen sections. If you end up with too many cells packed together, cut a thinner frozen section. If the end of the block seems a bit bare or you need to build up block, merely add more OCT around the block. The end of the block is where the cells are going to be as long as you don't add an enormous amount of OCT before snap freezing. Chris van der Loos also does this and can cryosection the most amazingly tiny block!! We have had zero luck using a collagen matrix to capture cells and went to the above method with far greater success. This is also a nice way to make positive controls for immunostaining or Beta Gal methods. Good luck Take At 12:19 PM 11/13/2006, you wrote: >Dear Histonet, > >My PI has been embedding cancer cells in collagen over the past several >weeks. My job is to take the collagen bead (approx 1-1.5 cm in diameter), >embed it in OCT and get frozen sections for H&E staining. Unfortunately, I >have been having a very hard time finding the cells in the collagen in the >OCT block because there is little to no contrast between the two. On top of >the that, the collagen detaches itself from the OCT as it hits the blade >leaving me with a square section of OCT that is missing a circle in the >middle (presumably where the collagen and cells would be if I could see >them). > >I have gone through several full specimens and have come away with only a >slide or two where I managed to catch some cells. This sort of inconsistency >is obviously not acceptable and my resources in the pathology department >have come up dry. Does anyone have any experience with this sort of >experiment? Is there any way to stain the collagen/cells in such a way that >I could at least tell where the cells are in the OCT block so I'm not just >blindly cutting away? Any help would be greatly appreciated. Thanks to >everyone in advance. > >Sincerely, > >Omer Richman >Research Technician >State University of New York at Stony Brook >Life Sciences Building Rm 004 >Stony Brook, NY 11794 >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From Nicholas_Gidmark <@t> brown.edu Mon Nov 13 13:54:48 2006 From: Nicholas_Gidmark <@t> brown.edu (Gidmark, Nicholas) Date: Mon Nov 13 13:56:34 2006 Subject: [Histonet] Nitrocellulose or other vertebrate cranial-mount medium? Message-ID: <6179894FC62F50438F1862775766F86904E257E7@MAIL2.AD.Brown.Edu> Hello all, I just started my doctoral degree at Brown University. At my last institution (U of MN), I used Low Viscosity Nitrocellulose (LVN) to embed whole (though small) fish heads. The biggest heads that I embedded were 4 cm X 3 cm X 2 cm. I am wondering if anyone knows of a good method to get a hold of nitrocellulose itself. We got our last shipment at the U of MN from G.J. Nikolas about three years ago, but they will no longer supply it for biological purposes. Because we've already bought EVERYTHING else here at Brown for the procedure, I'd rather not switch to a different embedding medium. But, if there's no other way to get nitrocellulose, I'm open to other methods of making whole mounts of vertebrate specimens of that size. Does anyone know other good methods of embedding vertebrate heads of that size, or how to get a hold of some nitrocellulose? As a side note, I've looked into the material that fisher sells, but the concentration that they have is, as I recall, about 5%. We were using final concentrations of close to 30% (100 grams dissolved in about 300 ml of ethanol/ether mix) , which would make that solution difficult to use, not to mention prohibitively expensive on that scale. Any ideas? Thanks for your help. --Nick Nicholas J. Gidmark, graduate student Brainerd lab of biomechanics and functional morphology Dept. of Ecology & Evolutionary Biology Brown University 80 Waterman Street, box G-W Providence, RI 02912 nicholas_gidmark@brown.edu Cell: 612.386.0042 Lab: 401.863.1032 From jane.moose <@t> newberryhospital.org Mon Nov 13 17:58:50 2006 From: jane.moose <@t> newberryhospital.org (Jane Moose) Date: Mon Nov 13 14:51:48 2006 Subject: [Histonet] CHRISTMAS TREE STAIN Message-ID: <010a01c7077f$aace8250$18041f0a@LAB2> Does anyone know of a supplier for a kit for staining sperm with the "Christmas Tree Stain." Does anyone have a procedure for making the stain? Thanks Jane Jane Moose LIS Coordinator Newberry County Memorial Hospital Newberry, SC 803-405-7129 ************************************************************************************ This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. From larfied <@t> yahoo.com Mon Nov 13 15:17:01 2006 From: larfied <@t> yahoo.com (Lawrence Fiedler) Date: Mon Nov 13 15:17:09 2006 Subject: [Histonet] urgent need for histo tech in South Florida Message-ID: <20061113211701.68948.qmail@web38203.mail.mud.yahoo.com> We are a group of 51 (and growing) gastroenterologists with our own new pathology lab. We are in need of a full time histo tech to work in our Western Broward County location. We are well aware of the national shortage of histo techs and will pay a premium! PLEASE CALL! Correspond to Dr. Lawrence Fiedler at Larfied@yahoo.com From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue Nov 14 02:21:21 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Nov 14 02:20:34 2006 Subject: [Histonet] Help Message-ID: <86ADE4EB583CE64799A9924684A0FBBFAFC95A@wahtntex2.waht.swest.nhs.uk> I think you are dehydrating your Von Kossa's correctly. After 20 to 30 sec in aqueous safranin O differentiate and dehydrate in 95% and 100% alcohol. Then clear with 100% alcohol and xylene, then two changes of xylene. As you know Von Kossa's demonstrates the presence of phosphates, soaps and amorphous but not crystalline carbonates, rather than calcium itself. Alcohol fixation is recommended as is neutral 10% formalin; any fixative containing calcium must obviously be avoided. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net There is no way to peace. Peace is the way. --A.J. Muste This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From LRaff <@t> lab.uropartners.com Tue Nov 14 08:02:22 2006 From: LRaff <@t> lab.uropartners.com (Lester Raff) Date: Tue Nov 14 08:02:33 2006 Subject: [Histonet] Paperless Message-ID: <5DA1CA5D0B98A84985B545A24423B822019A85@UPLAB01.uplab.local> Good morning. Here is a lab policy question. How many of your labs keep a paper copy on file of surgical path reports, and how many only keep an electronic copy? Thanks, Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue Nov 14 08:13:16 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Nov 14 08:12:26 2006 Subject: [Histonet] Paperless Message-ID: <86ADE4EB583CE64799A9924684A0FBBFAFC96B@wahtntex2.waht.swest.nhs.uk> Both...... But we are British and that is what you would expect. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net There is no way to peace. Peace is the way. --A.J. Muste This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From JWEEMS <@t> sjha.org Tue Nov 14 08:31:20 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Nov 14 08:31:36 2006 Subject: [Histonet] Paperless Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEF1E1@sjhaexc02.sjha.org> We have only electronic copies, and now only those are kept 10 years. Gives me heart failure! Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Lester Raff Sent: Tuesday, November 14, 2006 9:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paperless Good morning. Here is a lab policy question. How many of your labs keep a paper copy on file of surgical path reports, and how many only keep an electronic copy? Thanks, Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From Lynne.Bell <@t> hitchcock.org Tue Nov 14 08:31:41 2006 From: Lynne.Bell <@t> hitchcock.org (Bell, Lynne) Date: Tue Nov 14 08:34:12 2006 Subject: [Histonet] Paperless In-Reply-To: <5DA1CA5D0B98A84985B545A24423B822019A85@UPLAB01.uplab.local> Message-ID: We keep only an electronic copy. We do keep the original requisition now - but will be starting to scan those into the patient record soon. Lynne Bell, HT (ASCP) Central Vermont Medical Center Barre, VT 05641 From wsimms <@t> mcintoshclinic.com Tue Nov 14 08:34:44 2006 From: wsimms <@t> mcintoshclinic.com (Wesley Simms, MD) Date: Tue Nov 14 08:35:04 2006 Subject: [Histonet] Paperless References: <5DA1CA5D0B98A84985B545A24423B822019A85@UPLAB01.uplab.local> Message-ID: <001a01c707fa$0840ee80$530114ac@w3256domain.com> We only keep electronic copies. Wesley W. Simms, M.D. ----- Original Message ----- From: "Lester Raff" To: Sent: Tuesday, November 14, 2006 9:02 AM Subject: [Histonet] Paperless Good morning. Here is a lab policy question. How many of your labs keep a paper copy on file of surgical path reports, and how many only keep an electronic copy? Thanks, Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmcardle <@t> ebsciences.com Tue Nov 14 08:39:23 2006 From: pmcardle <@t> ebsciences.com (Phil McArdle) Date: Tue Nov 14 08:39:35 2006 Subject: [Histonet] CHRISTMAS TREE STAIN In-Reply-To: <010a01c7077f$aace8250$18041f0a@LAB2> References: <010a01c7077f$aace8250$18041f0a@LAB2> Message-ID: <4559D51B.8070707@ebsciences.com> Hello: Check out the following link; this isn't a kit, but there is a procedure: http://www.dfs.virginia.gov/services/forensicBiology/manuals/procedures/02%20-%20II-Presumptive%20and%20Confirmatory%20Tests/07%20-%203%20SEMEN%20IDENTIFICATION.pdf Best regards, Phil Jane Moose wrote: > Does anyone know of a supplier for a kit for staining sperm with the "Christmas Tree Stain." > > > Does anyone have a procedure for making the stain? > Thanks Jane > > > > > > > Jane Moose > LIS Coordinator > Newberry County Memorial Hospital > Newberry, SC > 803-405-7129 > > ************************************************************************************ > This message is intended only for the use of the individual or entity to > which it is addressed and may contain information that is privileged, > confidential and exempt from disclosure under applicable law. If the reader > of this message is not the intended recipient, you are hereby notified that > any dissemination, distribution, or copying of this communication is > strictly prohibited by law. If you have received this communication in > error, please notify me immediately. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Microwave Product Manager Energy Beam Sciences, Inc. 29-B Kripes Rd. East Granby, CT 06026 Tel: 800.992.9037 x 341 Fax: 860.653.0422 pmcardle@ebsciences.com www.ebsciences.com You must be the change you want to see in the world. - Mahatma Gandhi NOTE: This message, together with any attachments, is intended only for the use of the individual or entity to which it is addressed and may contain information that is legally privileged, confidential and exempt from disclosure. If you are not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this message, or any attachment, is strictly prohibited. If you have received this message in error, please notify the original sender immediately by telephone or by return E-mail and delete this message, along with any attachments, from your computer. Thank you. - Not Mahatma Gandhi From hymclab <@t> hyhc.com Tue Nov 14 08:51:00 2006 From: hymclab <@t> hyhc.com (hymclab) Date: Tue Nov 14 08:47:46 2006 Subject: [Histonet] Paperless Message-ID: The only hard paper copies we keep anymore are those that have attachments: -Frozen section diagnosis form (even though we enter the frozen section diagnosis in the computer in its own data sectin to become part of the permanent report, we keep a copy of the original document that was signed at the actual time of frozen section diagnosis). -Stone analysis reports that get attached to the final copy of the report. -Special studies sent out as part of the case (Disaccharidase studies, HER-2/neu Immunos that get sent out, Consultations, etc...) This has saved us a lot of space not saving hard copies of every path report. Hope this helps, Dawn -----Original Message----- From: Lester Raff [mailto:LRaff@lab.uropartners.com] Sent: Tuesday, November 14, 2006 8:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paperless Good morning. Here is a lab policy question. How many of your labs keep a paper copy on file of surgical path reports, and how many only keep an electronic copy? Thanks, Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From olek.michalski <@t> nencki.gov.pl Tue Nov 14 09:12:11 2006 From: olek.michalski <@t> nencki.gov.pl (Olek Michalski) Date: Tue Nov 14 09:10:32 2006 Subject: [Histonet] aquaporin 4 Message-ID: Hello Histonetters, I'm starting immuno for aquaporin 4 with Chemicon anti synthetic peptide (AB3068). After reading some papers describing use of this antibody it seems to me that it's fairly termolabile. Has anybody working protocol for this? Any advice is wellcome. Best regards Olek Michalski PS. Since the only secondary ab that fits my needs is produced in donkey and I don't have donkey serum I'm going to use horse serum. My coleague told me this works for some other stainings. Do you consider it really bad idea? From lblazek <@t> digestivespecialists.com Tue Nov 14 09:27:17 2006 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Nov 14 09:27:39 2006 Subject: [Histonet] Paperless Message-ID: <6CBA6DC98A079D408C87250591D9DFB802684BCA@bruexchange.digestivespecialists.com> We only keep an electronic copy. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lester Raff Sent: Tuesday, November 14, 2006 9:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paperless Good morning. Here is a lab policy question. How many of your labs keep a paper copy on file of surgical path reports, and how many only keep an electronic copy? Thanks, Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Tue Nov 14 09:35:48 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Nov 14 09:35:06 2006 Subject: AW: [Histonet] Paperless In-Reply-To: <5DA1CA5D0B98A84985B545A24423B822019A85@UPLAB01.uplab.local> Message-ID: <001601c70802$8f836be0$c812a8c0@dielangs.at> Both, the original print with the doc's signature is stored with the patients-history. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Lester Raff Gesendet: Dienstag, 14. November 2006 15:02 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Paperless Good morning. Here is a lab policy question. How many of your labs keep a paper copy on file of surgical path reports, and how many only keep an electronic copy? Thanks, Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Tue Nov 14 09:45:31 2006 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Tue Nov 14 09:45:40 2006 Subject: [Histonet] QIHC Message-ID: All, I have a tech that will be taking the QIHC exam soon and she is looking for feedback on what people that have taken it thought of the test. She recently took the HTL and was taken off gaurd by what was on that test (she still passed it though). Any comments would be very welcome. Thank-you, Glen Dawson IHC Manager Milwaukee, WI From Wanda.Smith <@t> HCAhealthcare.com Tue Nov 14 10:03:53 2006 From: Wanda.Smith <@t> HCAhealthcare.com (Smith Wanda) Date: Tue Nov 14 10:04:17 2006 Subject: [Histonet] Paperless In-Reply-To: <5DA1CA5D0B98A84985B545A24423B822019A85@UPLAB01.uplab.local> Message-ID: We keep the original requisition with all attachments in the department, and keep an electronic version of the final reports. Wanda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lester Raff Sent: Tuesday, November 14, 2006 9:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paperless Good morning. Here is a lab policy question. How many of your labs keep a paper copy on file of surgical path reports, and how many only keep an electronic copy? Thanks, Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgalnow <@t> aol.com Tue Nov 14 10:45:29 2006 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Tue Nov 14 10:45:44 2006 Subject: [Histonet] QIHC In-Reply-To: References: Message-ID: <8C8D6236D6C8943-9CC-11E4@WEBMAIL-MA11.sysops.aol.com> Glen, I took it about 3 months ago and it was a lot tougher than I thought it was going to be. I passed, but I didn't think I did at the time. Have her study the IHC quiz book from NSH, and to read the chapters on IHC in Sheehan and Carson books........ Roxanne Soto HT(ASCP)QIHC Lab Manager Physicians RightPath Tampa, Fl -----Original Message----- From: GDawson@dynacaremilwaukee.com To: histonet@lists.utsouthwestern.edu Sent: Tue, 14 Nov 2006 10:45 AM Subject: [Histonet] QIHC All, I have a tech that will be taking the QIHC exam soon and she is looking for feedback on what people that have taken it thought of the test. She recently took the HTL and was taken off gaurd by what was on that test (she still passed it though). Any comments would be very welcome. Thank-you, Glen Dawson IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From Heather.A.Harper <@t> pcola.med.navy.mil Tue Nov 14 10:49:42 2006 From: Heather.A.Harper <@t> pcola.med.navy.mil (Harper, Heather A., CIV) Date: Tue Nov 14 10:51:34 2006 Subject: [Histonet] Autopsy supply Message-ID: Hi Everybody, My histology dept. is in the morgue. I need some info. Does anyone know where I can get the disposable aprons that are also pants and boots? It's all just one piece and you slip it on and your shoes, pants are covered. Sort of looks like a jumpsuit. I have seen them and do not know the company who makes this and if anyone has any information or a link, please let me know. Thanks. Heather Naval Hospital Pensacola, FL From Dorothy.L.Webb <@t> HealthPartners.Com Tue Nov 14 10:52:22 2006 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Tue Nov 14 10:52:34 2006 Subject: [Histonet] controls Message-ID: <0E394B648E5284478A6CCB78E5AFDA2703640768@hpes1.HealthPartners.int> Does anyone know of a possible source to purchase ALK-1 controls? We cannot seem to come up with a good tissue control and have used CellMarque as our source, but, they are not available through them at this time. Appreciate any help in this area and thanks ahead of time!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From JEllin <@t> yumaregional.org Tue Nov 14 11:37:52 2006 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Tue Nov 14 11:38:40 2006 Subject: [Histonet] coding question Message-ID: <29BE166A2CF48D459853F8EC57CD37E8625988@EXCHANGECLUSTER.yumaregional.local> Hello everyone I have a question for the day, when recieveing in semen to document if sperm are present, how is everyone coding for this action. It seems to me that it would be a pathologist proffessional code, but we are creating the wet mount for the pathologist to see and is being reported on a pathology report. How are people handling this situation. Jesus A. Ellin HT ASCP Yuma Regional Medical Center Histology Systems Technologist Pathology Information Systems 928-336-7444 or 928-336-1144 Fax: 928-336-7319 This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. From Kim.Shields <@t> viha.ca Tue Nov 14 15:29:08 2006 From: Kim.Shields <@t> viha.ca (Shields, Kim) Date: Tue Nov 14 15:29:16 2006 Subject: [Histonet] IHC turn around times Message-ID: Hello, we currently do 150-200 IHC stains/day on two automated (Ventana) systems. Our bench also supports any deeper H&E requests, as well as manual & automated special stains. We have 1 tech working a 7.5 hr day. We have in the past been able to provide a 1 day TAT, but are finding it very difficult to keep up with the workload etc. So, we were wondering what other hospitals TAT for their IHC stains are???? Thanks in advance for any info you might provide. From LChen <@t> mednet.ucla.edu Tue Nov 14 15:55:05 2006 From: LChen <@t> mednet.ucla.edu (Chen, Leslie) Date: Tue Nov 14 15:55:39 2006 Subject: [Histonet] Removing hematoxylin from clothing Message-ID: <9F8AE7E7B303F44DB0CAB587F1E96C3F0A58CFAD@admedmail3.ad.medctr.ucla.edu> Hi, Does anybody know the best way to remove hematoxylin from clothing? Thanks. Leslie ---------------------------------------------------------- IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. ---------------------------------------------------------- From PMonfils <@t> Lifespan.org Tue Nov 14 16:32:29 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Tue Nov 14 16:32:40 2006 Subject: [Histonet] Removing hematoxylin from clothing Message-ID: <4EBFF65383B74D49995298C4976D1D5E273BAF@LSRIEXCH1.lsmaster.lifespan.org> I keep a little bottle of acid alcohol on the shelf above the washer. Stronger than you would usually use on slides, 4% to 5% HCl in 70% ethanol. I pour a litttle on the hematoxylin spot, rub it in a bit, let it sit a minute or so, then drop it into the already running washer. Works well on other basic dyes too, like safranin, basic fuchsin, etc. Of course on white clothing, bleach will remove many such dyes. But I use the acid alcohol on colored clothing that I don't want to bleach, and I usually use it on whites too, just as added insurance. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Chen, > Leslie > Sent: Tuesday, November 14, 2006 1:55 PM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] Removing hematoxylin from clothing > > Hi, > Does anybody know the best way to remove hematoxylin from clothing? > Thanks. > > > Leslie > > ---------------------------------------------------------- > IMPORTANT WARNING: This email (and any attachments) is only intended for > the use of the person or entity to which it is addressed, and may contain > information that is privileged and confidential. You, the recipient, are > obligated to maintain it in a safe, secure and confidential manner. > Unauthorized redisclosure or failure to maintain confidentiality may > subject you to federal and state penalties. If you are not the intended > recipient, please immediately notify us by return email, and delete this > message from your computer. > ---------------------------------------------------------- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From sjchtascp <@t> yahoo.com Tue Nov 14 17:28:58 2006 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Tue Nov 14 17:29:05 2006 Subject: [Histonet] 2 opening's in Milwaukee WI Message-ID: <20061114232858.7068.qmail@web38213.mail.mud.yahoo.com> 2 full time histo positions, 1 supervisor at Columbia St.Mary's in Milwaukee, WI. 1-414-961-3949 or 3948 --------------------------------- Cheap Talk? Check out Yahoo! Messenger's low PC-to-Phone call rates. From MVaughan4 <@t> ucok.edu Tue Nov 14 18:21:31 2006 From: MVaughan4 <@t> ucok.edu (MVaughan4@ucok.edu) Date: Tue Nov 14 18:22:02 2006 Subject: [Histonet] Cells Embedded in Collagen for Frozen Tissue Sectioning (Omer Richman) In-Reply-To: Message-ID: Omer, You don't mention whether you fix the cells in the collagen ahead of embedding. Also, do you infiltrate the collagen gel in 30% sucrose in PBS until the collagen sinks? That has always been our protocol and we had no problem sectioning collagen gels. I guess the bigger problem is, where are the cells in the collagen? And it looks like Gayle has a better answer than blindly sectioning collagen. Mel Melville B. Vaughan, Ph. D. Assistant Professor Department of Biology University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm Message: 6 Date: Mon, 13 Nov 2006 12:45:12 -0700 From: Gayle Callis Subject: Re: [Histonet] Cells Embedded in Collagen for Frozen Tissue Sectioning To: "Omer Richman" , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20061113123703.01b3b698@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed You do not need a collagen matrix to make a frozen cell block. Take 3 x 10 to the 7th ( higher power 7) cells suspended in PBS without any protein carrier. Spin down and wash 3 times with PBS, after the last spin, add approx 1 ml or less of OCT to the cell pellet, resuspend the cells. Snap freeze end of tube in liquid nitrogen and using a sharp rap on bottom of tube, dislodge the block, mount on a chuck and do frozen sections. If you end up with too many cells packed together, cut a thinner frozen section. If the end of the block seems a bit bare or you need to build up block, merely add more OCT around the block. The end of the block is where the cells are going to be as long as you don't add an enormous amount of OCT before snap freezing. Chris van der Loos also does this and can cryosection the most amazingly tiny block!! We have had zero luck using a collagen matrix to capture cells and went to the above method with far greater success. This is also a nice way to make positive controls for immunostaining or Beta Gal methods. Good luck Take At 12:19 PM 11/13/2006, you wrote: >Dear Histonet, > >My PI has been embedding cancer cells in collagen over the past several >weeks. My job is to take the collagen bead (approx 1-1.5 cm in diameter), >embed it in OCT and get frozen sections for H&E staining. Unfortunately, I >have been having a very hard time finding the cells in the collagen in the >OCT block because there is little to no contrast between the two. On top of >the that, the collagen detaches itself from the OCT as it hits the blade >leaving me with a square section of OCT that is missing a circle in the >middle (presumably where the collagen and cells would be if I could see >them). > >I have gone through several full specimens and have come away with only a >slide or two where I managed to catch some cells. This sort of inconsistency >is obviously not acceptable and my resources in the pathology department >have come up dry. Does anyone have any experience with this sort of >experiment? Is there any way to stain the collagen/cells in such a way that >I could at least tell where the cells are in the OCT block so I'm not just >blindly cutting away? Any help would be greatly appreciated. Thanks to >everyone in advance. > >Sincerely, > >Omer Richman >Research Technician >State University of New York at Stony Brook >Life Sciences Building Rm 004 >Stony Brook, NY 11794 >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From conniegrubaugh <@t> hotmail.com Tue Nov 14 18:26:02 2006 From: conniegrubaugh <@t> hotmail.com (connie grubaugh) Date: Tue Nov 14 18:26:14 2006 Subject: [Histonet] Paperless In-Reply-To: <001a01c707fa$0840ee80$530114ac@w3256domain.com> Message-ID: We keep both!!!!!!!!!!! >From: "Wesley Simms, MD" >To: "Lester Raff" >, >Subject: Re: [Histonet] Paperless >Date: Tue, 14 Nov 2006 09:34:44 -0500 > >We only keep electronic copies. >Wesley W. Simms, M.D. >----- Original Message ----- From: "Lester Raff" > >To: >Sent: Tuesday, November 14, 2006 9:02 AM >Subject: [Histonet] Paperless > > >Good morning. > > > >Here is a lab policy question. How many of your labs keep a paper copy >on file of surgical path reports, and how many only keep an electronic >copy? > > > >Thanks, > > > > > >Lester J. Raff, MD >Medical Director >UroPartners, LLC Laboratory > >2225 Enterprise Dr. Suite 2511 > >Westchester, IL 60154 > > >ph: 708-486-0076 >fax: 708-486-0080 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ All-in-one security and maintenance for your PC. Get a free 90-day trial! http://clk.atdmt.com/MSN/go/msnnkwlo0050000002msn/direct/01/?href=http://www.windowsonecare.com/?sc_cid=msn_hotmail From lpwenk <@t> sbcglobal.net Tue Nov 14 18:37:59 2006 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Tue Nov 14 18:38:18 2006 Subject: [Histonet] QIHC In-Reply-To: Message-ID: <001a01c7084e$4e603e30$beb92e4b@HPPav2> The Bancroft/Gamble book has several chapters on immuno. Another resource is the IHC handbook from Dako. You can get it from their webpage: http://www.dako.com/prod_productrelatedinformation?url=support_ihc_handbook. htm Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Tuesday, November 14, 2006 10:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] QIHC All, I have a tech that will be taking the QIHC exam soon and she is looking for feedback on what people that have taken it thought of the test. She recently took the HTL and was taken off gaurd by what was on that test (she still passed it though). Any comments would be very welcome. Thank-you, Glen Dawson IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From orichman <@t> gmail.com Tue Nov 14 20:37:54 2006 From: orichman <@t> gmail.com (Omer Richman) Date: Tue Nov 14 20:38:03 2006 Subject: [Histonet] Cells Embedded in Collagen for Frozen Tissue Sectioning (Omer Richman) In-Reply-To: References: Message-ID: <5942d2e00611141837r78f1109eo53ace7ed8a9d0115@mail.gmail.com> I apologize for leaving out some critical elements, I had only a few moments to type that up. The cells are pre-fixed in the collagen prior to embedding and we do wait until the collagen sinks (generally overnight). The cells are in the center of the collagen in a ball. Prior to freezing in OCT, it's easy to see them but once it's in the OCT block I find it difficult to distinguish them from the rest of the collagen. Also, the collagen separates from the rest of the OCT during sectioning so that, even if I could find the cells, I feel I may lose them during the act of cutting. That leads me to believe that perhaps I'm not using the right temperature. At what temperature do you normally cut collagen? Gayle's answer actually helped in a related experiment so I extend my thanks to her for that. However, my PI reminded me we need to use collagen because we want to see how far the cells migrate then follow that up with IHC. Thank you all again for your assistance. Omer Richman Research Technician State University of New York at Stony Brook Life Sciences Building Rm 004 Stony Brook, NY 11794 On 11/14/06, MVaughan4@ucok.edu wrote: > > Omer, > You don't mention whether you fix the cells in the collagen ahead of > embedding. Also, do you infiltrate the collagen gel in 30% sucrose in PBS > until the collagen sinks? That has always been our protocol and we had no > problem sectioning collagen gels. I guess the bigger problem is, where > are the cells in the collagen? And it looks like Gayle has a better answer > than blindly sectioning collagen. > Mel > > Melville B. Vaughan, Ph. D. > Assistant Professor > Department of Biology > University of Central Oklahoma > 100 N. University Drive > Edmond, OK 73034 > http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm > > > From hodges420 <@t> msn.com Tue Nov 14 21:19:19 2006 From: hodges420 <@t> msn.com (MARY T HODGES) Date: Tue Nov 14 21:19:30 2006 Subject: FW: [Histonet] IHC turn around times Message-ID: Does any one use potassium ferrocinide (miss spell ) sorry with trihydrate in it or jsut PF Olease let me know TereHodges ______________________________________________________________ From: "Shields, Kim" To: Subject: [Histonet] IHC turn around times Date: Tue, 14 Nov 2006 13:29:08 -0800 >Hello, we currently do 150-200 IHC stains/day on two automated (Ventana) systems. Our bench also supports any deeper H&E requests, as well as manual & automated special stains. >We have 1 tech working a 7.5 hr day. We have in the past been able to provide a 1 day TAT, but are finding it very difficult to keep up with the workload etc. >So, we were wondering what other hospitals TAT for their IHC stains are???? > >Thanks in advance for any info you might provide. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dassog <@t> evergreen.edu Tue Nov 14 22:42:46 2006 From: dassog <@t> evergreen.edu (Dasso, Greg (staff)) Date: Tue Nov 14 22:46:18 2006 Subject: [Histonet] IHC turn around times References: Message-ID: <3872E8431D06E545AC955BED177CB6F601604B64@oak.evergreen.edu> Potassium Ferrocyanide, in its non-anhydrous form, is as a trihydrate. The formula for your salt should be :K4Fe(CN)6.3H2O. The dot between the (CN)6 and the 3H2O simply means that there are 3 "waters of hydration" in the stable salt. Greg -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of MARY T HODGES Sent: Tue 11/14/2006 7:19 PM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] IHC turn around times Does any one use potassium ferrocinide (miss spell ) sorry with trihydrate in it or jsut PF Olease let me know TereHodges ______________________________________________________________ From: "Shields, Kim" To: Subject: [Histonet] IHC turn around times Date: Tue, 14 Nov 2006 13:29:08 -0800 >Hello, we currently do 150-200 IHC stains/day on two automated (Ventana) systems. Our bench also supports any deeper H&E requests, as well as manual & automated special stains. >We have 1 tech working a 7.5 hr day. We have in the past been able to provide a 1 day TAT, but are finding it very difficult to keep up with the workload etc. >So, we were wondering what other hospitals TAT for their IHC stains are???? > >Thanks in advance for any info you might provide. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Nov 15 02:12:25 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Nov 15 03:11:12 2006 Subject: [Histonet] Removing hematoxylin from clothing Message-ID: <86ADE4EB583CE64799A9924684A0FBBFAFC976@wahtntex2.waht.swest.nhs.uk> Interestingly wasn't that the original job of mordanted stains; to stain textiles. Why don't you tie the garment in places and fully immerse in the stain, wash, dry and voila, tie/ die garments make a return. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net There is no way to peace. Peace is the way. --A.J. Muste This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From sahibzan <@t> georgetown.edu Wed Nov 15 07:49:43 2006 From: sahibzan <@t> georgetown.edu (Niaz Sahibzada) Date: Wed Nov 15 07:49:52 2006 Subject: [Histonet] Will Xylene destroy BDA Message-ID: Hi All! I have injected 10% Biotinylated Dextran Amine (BDA, 10k M.W.) into a CNS nucleus hoping to see anterograde label in the periphery (i.e., smooth muscle). Since I need 2?m sections, paraffin embeding is the only way to go. Does anybody know if the procedure for deparaffinization will adversely effect the BDA label (i.e. Destroy the biotin)? Please advice. ********************************* Niaz Sahibzada, Ph. D. Associate Professor Department of Pharmacology Rm. #410 NW?Med-Dent Bldg. Georgetown University Medical Center 3900 Reservoir Road, NW Washington, D. C. 20057 Tel: (202) 687-1500 e-mail: sahibzan@georgetown.edu ********************************* CONFIDENTIALITY NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately. From Kathy.Johnston <@t> CLS.ab.ca Wed Nov 15 07:58:22 2006 From: Kathy.Johnston <@t> CLS.ab.ca (Kathy.Johnston@CLS.ab.ca) Date: Wed Nov 15 07:58:58 2006 Subject: [Histonet] Removing hematoxylin from clothing In-Reply-To: <9F8AE7E7B303F44DB0CAB587F1E96C3F0A58CFAD@admedmail3.ad.medctr.ucla.edu> Message-ID: <4BC300747AF87A48BCDF8E48BC2885CE0136546E@mail1.calgary.com> If you can get your hands on it, Erado-sol works wonderful on clothing, and won't bleach out colored fabrics. Kathy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Chen, Leslie Sent: November 14, 2006 2:55 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Removing hematoxylin from clothing Hi, Does anybody know the best way to remove hematoxylin from clothing? Thanks. Leslie ---------------------------------------------------------- IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. ---------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. From naje1972 <@t> yahoo.com Wed Nov 15 08:06:35 2006 From: naje1972 <@t> yahoo.com (cynthia haynes) Date: Wed Nov 15 08:06:47 2006 Subject: [Histonet] Full time or part time positions Message-ID: <963686.79361.qm@web33001.mail.mud.yahoo.com> This message is for laboratories in the Chicago area only. I am looking a for full or part-time in Histology. I have 26+ years of experience in Histology, 10+ years of Immunohistochemistry experience, my specialities are eyes, brain, bones and teeth. I prefer early morning hours but will consider other times. I can be reached at this e-mail or you may call me at 312-919-9887. Thank you Cynthia Haynes ____________________________________________________________________________________ Sponsored Link $420k for $1,399/mo. Think You Pay Too Much For Your Mortgage? Find Out! www.LowerMyBills.com/lre From GDawson <@t> dynacaremilwaukee.com Wed Nov 15 08:23:08 2006 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Wed Nov 15 08:23:18 2006 Subject: [Histonet] IHC turn around times Message-ID: Kim, My department performs IHC, IF & In-situ testing. Our IHC workload is the same as yours & our turn around time is: Same day results for requests received by 11:00am but I have 2 techs during the day so more than one 8 hour shift is covered. All orders received after 11:00 am are done the following morning. Hope this helps, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Shields, Kim Sent: Tuesday, November 14, 2006 3:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC turn around times Hello, we currently do 150-200 IHC stains/day on two automated (Ventana) systems. Our bench also supports any deeper H&E requests, as well as manual & automated special stains. We have 1 tech working a 7.5 hr day. We have in the past been able to provide a 1 day TAT, but are finding it very difficult to keep up with the workload etc. So, we were wondering what other hospitals TAT for their IHC stains are???? Thanks in advance for any info you might provide. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wia2005 <@t> med.cornell.edu Wed Nov 15 08:35:50 2006 From: wia2005 <@t> med.cornell.edu (William Ares) Date: Wed Nov 15 08:36:23 2006 Subject: [Histonet] EAAT-4 and nGLAST immuno Message-ID: <4ff1d786503.455adf76@med.cornell.edu> I am attempting EAAT-4 and nGLAST immuno on paraffin embedded slices of p7 mouse brain without any success whatsoever. Literature searches have given me primary concentrations of 1:200 to 1:2000 (didn't work) and I have tried concentrations as high as 1:25 (still didn't work). Does anyone have any tips or tricks for me that may end with good results? Thanks. William Ares Research Technician Labratory of Molecular and Developmental Neuroscience Weill Medical College of Cornell University Tel: (212) 746 5056 Email: wia2005@med.cornell.edu From SDrew <@t> uwhealth.org Wed Nov 15 08:47:19 2006 From: SDrew <@t> uwhealth.org (Drew Sally A.) Date: Wed Nov 15 08:47:52 2006 Subject: [Histonet] IHC-TAT & Pathologists' hours Message-ID: As I was reading about other peoples' TAT policies, what kind of hours do your pathologists keep? Are their hours staggered also so someone is available to read the immunos as they come off? Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 (608)265-6596 From naje1972 <@t> yahoo.com Wed Nov 15 08:06:40 2006 From: naje1972 <@t> yahoo.com (cynthia haynes) Date: Wed Nov 15 09:06:59 2006 Subject: [Histonet] Full time or part time positions Message-ID: <322883.34600.qm@web33008.mail.mud.yahoo.com> This message is for laboratories in the Chicago area only. I am looking a for full or part-time in Histology. I have 26+ years of experience in Histology, 10+ years of Immunohistochemistry experience, my specialities are eyes, brain, bones and teeth. I prefer early morning hours but will consider other times. I can be reached at this e-mail or you may call me at 312-919-9887. Thank you Cynthia Haynes ____________________________________________________________________________________ Sponsored Link Mortgage rates near 39yr lows. $510k for $1,698/mo. Calculate new payment! www.LowerMyBills.com/lre From gcallis <@t> montana.edu Wed Nov 15 10:19:04 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Nov 15 10:19:34 2006 Subject: [Histonet] Cells Embedded in Collagen for Frozen Tissue Sectioning (Omer Richman) In-Reply-To: <5942d2e00611141837r78f1109eo53ace7ed8a9d0115@mail.gmail.co m> References: <5942d2e00611141837r78f1109eo53ace7ed8a9d0115@mail.gmail.com> Message-ID: <6.0.0.22.1.20061115090748.01b79af8@gemini.msu.montana.edu> Omer, You did not say what temperature you were cryosectioning at? But if the temperature is too cold and the collagen is separating from the little ball, then warm up the temperature with the same temperature for both block and blade. A perfectly round ball i.e your collagen balls have always been more difficult to handle so they do not curl at sectioning. We found this out in our lab too. Whya that little ball curls must be some mechanical problem due to the round shape versus a shape with some type of point on it. We did agar type balls both as frozen sections and paraffin sections and both curled up and out of the both types of embedding medias. You may want to try a different cryoembedding media, Thermo Electron has one which is supposed to work nicely for colder temperatures. Andy Grantham and Teri Johnson have used this one - it may help, and it was recently mentioned by Andy in a Histonet messAGe. Also when embedding, make sure you blot the excess sucrose off and let the balls sit in OCT for a short time before snap freezing - you may get a better interface of collagen to embedding media. Another alternative to keep the balls from curling is using the Instrumedics tape transfer Cryojane - but not everyone has this instrument, but the tape would keep the balls from curling up. Good luck on solving your frustrations Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 At 07:37 PM 11/14/2006, you wrote: >I apologize for leaving out some critical elements, I had only a few moments >to type that up. The cells are pre-fixed in the collagen prior to embedding >and we do wait until the collagen sinks (generally overnight). The cells are >in the center of the collagen in a ball. Prior to freezing in OCT, it's easy >to see them but once it's in the OCT block I find it difficult to >distinguish them from the rest of the collagen. Also, the collagen separates >from the rest of the OCT during sectioning so that, even if I could find the >cells, I feel I may lose them during the act of cutting. That leads me to >believe that perhaps I'm not using the right temperature. At what >temperature do you normally cut collagen? > >Gayle's answer actually helped in a related experiment so I extend my thanks >to her for that. However, my PI reminded me we need to use collagen because >we want to see how far the cells migrate then follow that up with IHC. Thank >you all again for your assistance. > >Omer Richman >Research Technician >State University of New York at Stony Brook >Life Sciences Building Rm 004 >Stony Brook, NY 11794 > >On 11/14/06, MVaughan4@ucok.edu wrote: >> >>Omer, >>You don't mention whether you fix the cells in the collagen ahead of >>embedding. Also, do you infiltrate the collagen gel in 30% sucrose in PBS >>until the collagen sinks? That has always been our protocol and we had no >>problem sectioning collagen gels. I guess the bigger problem is, where >>are the cells in the collagen? And it looks like Gayle has a better answer >>than blindly sectioning collagen. >>Mel >> >>Melville B. Vaughan, Ph. D. >>Assistant Professor >>Department of Biology >>University of Central Oklahoma >>100 N. University Drive >>Edmond, OK 73034 >>http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm >> From gcallis <@t> montana.edu Wed Nov 15 10:53:08 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Nov 15 10:53:24 2006 Subject: [Histonet] ATTN: Veterinary Diagnostic Laboratories, Revisiting Bovine viral diarrhea antibody, BVD Message-ID: <6.0.0.22.1.20061115093239.01b53bf8@gemini.msu.montana.edu> Dear All, Our veterinary diagnostic laboratory is looking for more clones (monoclonal) or a polyclonal BVD antibody which is consistent and reliable. In doing a Histonet Archive search, it seems some labs have experienced staining problems and they were looking for the antibody also. They need both unconjugated and a FITC conjugate. It has been a long time since I had to work with antiBVD but did have success with mouse anti BVD, clone 15c.5 from Cornell. It has been a long time since we purchased this and I am not sure you buy it from Cornell anymore?? One polyclonal they are using now for cell cultures is a swine antiBVD-FITC. Any suggestions for other antibodies out there will be welcomed. I would like to know what kits laboratories prefer to use for enzyme immunohistochemistry along with a favored enzyme digestion on FFPE tissues with this antibody. For research purposes, my lab did not use kits and never had any staining problems but the diagnostic lab wants the convenience of using kits and AEC chromogen. Thanks in advance Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From pruegg <@t> ihctech.net Wed Nov 15 11:54:16 2006 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Nov 15 11:55:00 2006 Subject: [Histonet] Removing hematoxylin from clothing In-Reply-To: <4BC300747AF87A48BCDF8E48BC2885CE0136546E@mail1.calgary.com> Message-ID: <001d01c708df$12a745f0$6501a8c0@Patsy> In my laundry room at home I keep a bottle of dilute acid alcohol to remove hematoxylin stain. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy.Johnston@CLS.ab.ca Sent: Wednesday, November 15, 2006 6:58 AM To: LChen@mednet.ucla.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Removing hematoxylin from clothing If you can get your hands on it, Erado-sol works wonderful on clothing, and won't bleach out colored fabrics. Kathy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Chen, Leslie Sent: November 14, 2006 2:55 PM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Removing hematoxylin from clothing Hi, Does anybody know the best way to remove hematoxylin from clothing? Thanks. Leslie ---------------------------------------------------------- IMPORTANT WARNING: This email (and any attachments) is only intended for the use of the person or entity to which it is addressed, and may contain information that is privileged and confidential. You, the recipient, are obligated to maintain it in a safe, secure and confidential manner. Unauthorized redisclosure or failure to maintain confidentiality may subject you to federal and state penalties. If you are not the intended recipient, please immediately notify us by return email, and delete this message from your computer. ---------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Wed Nov 15 11:56:17 2006 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Nov 15 11:57:09 2006 Subject: [Histonet] QIHC In-Reply-To: <001a01c7084e$4e603e30$beb92e4b@HPPav2> Message-ID: <001e01c708df$5ac18670$6501a8c0@Patsy> Glen, You might want to suggest that she joins the NSH IHC Resource Group at www.ihcrg.org we have lots of resources for those taking the QIHC exam. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Tuesday, November 14, 2006 5:38 PM To: 'Dawson, Glen'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] QIHC The Bancroft/Gamble book has several chapters on immuno. Another resource is the IHC handbook from Dako. You can get it from their webpage: http://www.dako.com/prod_productrelatedinformation?url=support_ihc_handbook. htm Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Tuesday, November 14, 2006 10:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] QIHC All, I have a tech that will be taking the QIHC exam soon and she is looking for feedback on what people that have taken it thought of the test. She recently took the HTL and was taken off gaurd by what was on that test (she still passed it though). Any comments would be very welcome. Thank-you, Glen Dawson IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Wed Nov 15 12:05:09 2006 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Nov 15 12:05:53 2006 Subject: [Histonet] Cells Embedded in Collagen for Frozen Tissue Sectioning(Omer Richman) In-Reply-To: Message-ID: <001f01c708e0$97bb4ec0$6501a8c0@Patsy> Another option is to wash the cells in PBS, then suspend in liquefied Histogel (warm the histogel til it is liquid), let the Histogel set up (usually 5-10 min) at RT, you can then put the tube in a beaker of warm water and turn it upside down and tap the tube and the cell block will fall out as a solid plug. You can either snap freeze the cell block or wrap in paper and process into paraffin. I do this all the time, but if I am processing into paraffin, I suspend the cells in formalin to fix, spin them down and take off the formalin then was a few times with PBS by resuspending and spinning before suspending in the liquid Histogel. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MVaughan4@ucok.edu Sent: Tuesday, November 14, 2006 5:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cells Embedded in Collagen for Frozen Tissue Sectioning(Omer Richman) Omer, You don't mention whether you fix the cells in the collagen ahead of embedding. Also, do you infiltrate the collagen gel in 30% sucrose in PBS until the collagen sinks? That has always been our protocol and we had no problem sectioning collagen gels. I guess the bigger problem is, where are the cells in the collagen? And it looks like Gayle has a better answer than blindly sectioning collagen. Mel Melville B. Vaughan, Ph. D. Assistant Professor Department of Biology University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm Message: 6 Date: Mon, 13 Nov 2006 12:45:12 -0700 From: Gayle Callis Subject: Re: [Histonet] Cells Embedded in Collagen for Frozen Tissue Sectioning To: "Omer Richman" , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20061113123703.01b3b698@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed You do not need a collagen matrix to make a frozen cell block. Take 3 x 10 to the 7th ( higher power 7) cells suspended in PBS without any protein carrier. Spin down and wash 3 times with PBS, after the last spin, add approx 1 ml or less of OCT to the cell pellet, resuspend the cells. Snap freeze end of tube in liquid nitrogen and using a sharp rap on bottom of tube, dislodge the block, mount on a chuck and do frozen sections. If you end up with too many cells packed together, cut a thinner frozen section. If the end of the block seems a bit bare or you need to build up block, merely add more OCT around the block. The end of the block is where the cells are going to be as long as you don't add an enormous amount of OCT before snap freezing. Chris van der Loos also does this and can cryosection the most amazingly tiny block!! We have had zero luck using a collagen matrix to capture cells and went to the above method with far greater success. This is also a nice way to make positive controls for immunostaining or Beta Gal methods. Good luck Take At 12:19 PM 11/13/2006, you wrote: >Dear Histonet, > >My PI has been embedding cancer cells in collagen over the past several >weeks. My job is to take the collagen bead (approx 1-1.5 cm in diameter), >embed it in OCT and get frozen sections for H&E staining. Unfortunately, I >have been having a very hard time finding the cells in the collagen in the >OCT block because there is little to no contrast between the two. On top of >the that, the collagen detaches itself from the OCT as it hits the blade >leaving me with a square section of OCT that is missing a circle in the >middle (presumably where the collagen and cells would be if I could see >them). > >I have gone through several full specimens and have come away with only a >slide or two where I managed to catch some cells. This sort of inconsistency >is obviously not acceptable and my resources in the pathology department >have come up dry. Does anyone have any experience with this sort of >experiment? Is there any way to stain the collagen/cells in such a way that >I could at least tell where the cells are in the OCT block so I'm not just >blindly cutting away? Any help would be greatly appreciated. Thanks to >everyone in advance. > >Sincerely, > >Omer Richman >Research Technician >State University of New York at Stony Brook >Life Sciences Building Rm 004 >Stony Brook, NY 11794 >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtarango <@t> nvcancer.org Wed Nov 15 14:00:40 2006 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Wed Nov 15 14:01:43 2006 Subject: [Histonet] Seminoma, Embryonic Carcinoma Message-ID: <5AEC610C1CE02945BD63A395BA763EDEC9A91C@NVCIEXCH02.NVCI.org> Hi everyone, Does anyone have seminoma, embryonic carcinoma, or any other germ cell tumor that they can share for controls? Thanks Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 "MMS " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From carl.hobbs <@t> kcl.ac.uk Wed Nov 15 13:37:58 2006 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Wed Nov 15 14:05:45 2006 Subject: [Histonet] re: EAAT4 Message-ID: <001601c708ed$8de2a260$0201a8c0@carlba65530bda> Hi William. which AB are you using and..what area/s of the brain are you looking at? EAAT4 has a extremely localised distribution ;-) Best wishes carl From carolb <@t> phys.mcw.edu Wed Nov 15 14:10:23 2006 From: carolb <@t> phys.mcw.edu (Bobrowitz, Carol) Date: Wed Nov 15 14:12:23 2006 Subject: [Histonet] tissue-tek mega cassettes Message-ID: <8F78639AC56F4143B267FE5F5A1B92C80E43AA@guyton.phys.mcw.edu> Hello, I've been purchasing from Sakura the tissue-tek mega cassettes. I use the HM355S Microm microtome. These cassettes were the only ones I could find to purchase that fit the microtome chuck. I tested several other makes all were loose fitting and caused sectioning problems. Sakura has changed the design of their tissue-tek mega cassettes. They no longer fit snuggly in the chuck. If you put the block in horizontally or vertically it does not fit correctly. Has anyone else had this problem? If I use a piece of small cardboard, like the old days, it wouldn't work. The vertical measurement is too short for a snug fit. I need a mega cassette that fits snuggly in the chuck. Does anyone purchase these without this problem? May I please have the name of your source? Any vendor who sell mega cassettes 1 ? " by 1" and ?" deep please send me some samples to test. Any help would be appreciated. Thank you, Carol Ann Bobrowitz Medical College of Wisconsin Department of Physiology MEB RM #4940 8701 Watertown Plank Road Milwaukee, Wisconsin 53226 cbobrowi@mcw.edu From gu.lang <@t> gmx.at Wed Nov 15 14:19:41 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Nov 15 14:19:24 2006 Subject: [Histonet] PTEN and Mammaglobin Message-ID: <001501c708f3$62172e70$c812a8c0@dielangs.at> Hi, has anyone success with the PTEN and Mammaglobin from Dako on the Ventana Benchmark XT? Could you share your protocol with me? Regards Gudrun Lang From gcallis <@t> montana.edu Wed Nov 15 15:06:58 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Nov 15 15:41:43 2006 Subject: [Histonet] tissue-tek mega cassettes In-Reply-To: <8F78639AC56F4143B267FE5F5A1B92C80E43AA@guyton.phys.mcw.edu > References: <8F78639AC56F4143B267FE5F5A1B92C80E43AA@guyton.phys.mcw.edu> Message-ID: <6.0.0.22.1.20061115133516.01b2cee8@gemini.msu.montana.edu> Carol, My long standing frustrations will be showing up in this message with what I consider an engineering problem. I have never found a mega cassette that fits in any of my microtome block holders. I have tried so many of them and they were ALL painfully useless. I listened to various vendors and tried their sample so generously supplied to the lab, but their mega cassettes failed to fit in the microtome holder. I have actually spent time comparing a regular cassette design to a mega cassette design, how they fit in the holder - it was an interesting exercise since they do NOT fit the same way although they LOOK the same. I've groused about this over the years one one with vendors. But nothing has changed since the mega cassettes are still a poor, loose fit in the holders. I have had them fall OUT onto my blade !@#%!@$@#$ with vertical orientation and slide side to side with horizonal orientatio My mega cassettes were made by Sakura many years ago but these have never been a good fit in my holders but then no other vendors have worked either. I don't know how much vendors test this product, but many histotechs have experienced the problem. Histonet has some extensive discussion on this so it is not a new problem that has been around for years. Our lab gave up using them except for processing large whole rat knees, whole mouse heads, or oversized tissues. They are tossed in the garbage at embedding to use another setup with large Peelaway molds and regular cassette backs which produces a firmly held, stable block at sectioning. I am ever hopeful one will be manufactured that truly fits in a holder (ALL HOLDERS) and does the job! I wish you luck in finding the product, and if you do, let us know. Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 At 01:10 PM 11/15/2006, you wrote: >Hello, > > > >I've been purchasing from Sakura the tissue-tek mega cassettes. > >I use the HM355S Microm microtome. > >These cassettes were the only ones I could find to purchase that fit the >microtome chuck. > >I tested several other makes all were loose fitting and caused sectioning >problems. > > > >Sakura has changed the design of their tissue-tek mega cassettes. > >They no longer fit snuggly in the chuck. If you put the block in >horizontally or vertically > >it does not fit correctly. > > > >Has anyone else had this problem? > > > >If I use a piece of small cardboard, like the old days, it wouldn't work. > >The vertical measurement is too short for a snug fit. > > > >I need a mega cassette that fits snuggly in the chuck. Does anyone >purchase these > >without this problem? > > > >May I please have the name of your source? > > > >Any vendor who sell mega cassettes 1 ? " by 1" and ?" deep please send me >some > >samples to test. > > > >Any help would be appreciated. > > > >Thank you, > > > >Carol Ann Bobrowitz > >Medical College of Wisconsin > >Department of Physiology > >MEB RM #4940 > >8701 Watertown Plank Road > >Milwaukee, Wisconsin 53226 > > > >cbobrowi@mcw.edu > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From contact <@t> excaliburpathology.com Wed Nov 15 16:40:33 2006 From: contact <@t> excaliburpathology.com (P Pierce) Date: Wed Nov 15 16:40:43 2006 Subject: [Histonet] tissue tek mega cassettes Message-ID: <20061115224033.42320.qmail@web50109.mail.yahoo.com> I was so excited when they finally made the mega cassettes. Before them, I used teabags to process and L-blocks to embed rabbit, dog, and smaller human eyes. I still use an old AO 820 with a Tissue-Tek quick release block holder. The block holder can be adjusted by holding up the bottom and turning the knob at the top to make it fit. I have even noticed differences in regular cassettes from different companies and just adjust as needed. Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-570-6679 405-759-3953 contact@excaliburpathology.com ____________________________________________________________________________________ Sponsored Link Mortgage rates near 39yr lows. $510k for $1,698/mo. Calculate new payment! www.LowerMyBills.com/lre From billions <@t> public1.sz.js.cn Thu Nov 16 00:22:47 2006 From: billions <@t> public1.sz.js.cn (Sinoera Tech) Date: Thu Nov 16 00:23:16 2006 Subject: [Histonet] Re: Alcian Yellow & Alcian Blue 8GX Message-ID: <004401c70947$a45b3fc0$7501a8c0@sinoera63d3596> Dear Histonetters, We are manufacturer of Alcian Yellow, Alcian Blue 8GX, Alcian Green, o-Cresolphthalein monophosphate disodium salt o-Cresolphthalein monophosphate N-methylglucammonium salt 1-Naphtholphthalein monophosphate 1-Naphtholphthalein monophosphate disodium salt Phenolphthalein disulfate tripotassium salt CAS NO.62625-16-5 3',3'',5',5''-Tetrachlorophenol-3,4,5,6-tetrabromosulfophthalein Indocyanine green IR-820 Please contact us for requirement. Kind Regards. - SUZHOU SINOERA CHEM CO., LTD. 125 Binhe Road Suzhou New & Hi-Tech District 215011 China Fax: +86 512 68258994 Tel: +86 512 68246939 http://www.sinoeratech.com http://www.sinoerachem.com From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Nov 16 02:19:35 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Nov 16 02:18:42 2006 Subject: [Histonet] Re: Alcian Yellow & Alcian Blue 8GX Message-ID: <86ADE4EB583CE64799A9924684A0FBBFAFC98B@wahtntex2.waht.swest.nhs.uk> I've got a bike for sale, 6 back gears, 3 front with a padded seat, ?200 plus packaging and postage. Please contact me on the below number if you want a test drive. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net A belief is purely an individual matter, and you cannot and must not organize it. --J. Krishnamurti This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Nov 16 02:20:47 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Nov 16 02:19:51 2006 Subject: [Histonet] Seminoma, Embryonic Carcinoma Message-ID: <86ADE4EB583CE64799A9924684A0FBBFAFC98C@wahtntex2.waht.swest.nhs.uk> Hi everyone, Does anyone have seminoma, embryonic carcinoma, or any other germ cell tumor that they can share for controls? Sorry I had them removed. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net A belief is purely an individual matter, and you cannot and must not organize it. --J. Krishnamurti This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Thu Nov 16 02:29:35 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Nov 16 02:28:41 2006 Subject: [Histonet] Re: Cut resistant gloves Message-ID: <86ADE4EB583CE64799A9924684A0FBBFAFC98E@wahtntex2.waht.swest.nhs.uk> Had an Aprilia (silver race standard one) for a while; son had to learn on a 125cc to get his tests so I downsized from a BMW 750. The little Aprilia was 'manic' and did over 100 mph, but needed an engine rebuild after about 15,000 miles. In the UK Aprilia's are 18 years old bikes and rarely last 12 months; my son sold his at a good price when it was 4 years old. He now owns a Yamaha R6; I know, I know, they are dangerous but he doesn't ride ii in the Winter and has all the armour. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net A belief is purely an individual matter, and you cannot and must not organize it. --J. Krishnamurti This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From RSRICHMOND <@t> aol.com Thu Nov 16 12:43:01 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Thu Nov 16 12:49:20 2006 Subject: [Histonet] Re: Alcian Yellow & Alcian Blue 8GX Message-ID: We have a solicitation from Suzhou Sinoera Chem Co. in Suzhou New & Hi-Tech District in China about various Alcian dyes. (Actually they spam me every month or so with similar advertisements.) Many of you have been following Dick Dapson's efforts at Anatech to develop environmentally safe processes for making these dyes, after the original German manufacturer stopped making them because they found the synthetic intermediates too hazardous to handle. Anatech now offers environmentally safely synthesized Alcian blue, but not the other dyes (unless I'm behind in my reading). Does Suzhou Sinoera use synthetic methods that are safe for workers and for the environment? (I have no connection with Anatech or with Suzhou Sinoera.) Bob Richmond Knoxville, Tennessee From GDawson <@t> dynacaremilwaukee.com Thu Nov 16 14:44:58 2006 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Thu Nov 16 14:45:06 2006 Subject: [Histonet] HTL Comments Message-ID: All, I have 2 techs that took the HTL in January of this year & many have asked for info/comments on the test so I'm turning over this email posting to them: Katie Wertz & Melissa Cowman: We studied Sheehan, Carson and the BOR study guide and they were fairly useless. The vast majority of the test included IHC for Melissa and Plastics for Katie, as well as many management questions about training. Out of the thousand questions from the HT and HTL portion of the BOR study guide, only a few actually applied to the questions found on the written. The management questions were about safety situations in which a technician would need further training. The pictures were small, grainy and in some cases unrecognizable. The pictures (with the exception of one) did not come from either Sheehan or Carson. Experience in the lab was a big help in answering many questions. There were also hand drawn pictures of anatomical structures where you needed to identify specific parts. We both had a drawing of the eye. We both felt like crying at the end of the test, but we passed! Hope this helps, Melissa and Katie From barrickstacey <@t> yahoo.com Thu Nov 16 13:03:48 2006 From: barrickstacey <@t> yahoo.com (Stacey Barrick) Date: Thu Nov 16 14:50:44 2006 Subject: [Histonet] freezing brain slices Message-ID: <20061116190348.30064.qmail@web54310.mail.yahoo.com> Hello.. I'm trying to freeze brain slices for immuno. The slices are 250uM cut with a vibratome. I have been trying to embed these slices in OCT so that I may section them using the cryostat. It seems as though the tissue is not freezing flat. Does anyone have any suggestions? I cannot get the slices to freeze flat no matter what i try. Thank you! Stacey barrickstacey@yahoo.com --------------------------------- Sponsored Link $420,000 Mortgage for $1,399/month - Think You Pay Too Much For Your Mortgage? Find Out! From barrickstacey <@t> yahoo.com Thu Nov 16 13:15:23 2006 From: barrickstacey <@t> yahoo.com (Stacey Barrick) Date: Thu Nov 16 15:02:11 2006 Subject: [Histonet] freezing brain slices Message-ID: <20061116191523.81406.qmail@web54312.mail.yahoo.com> Hello.. I'm trying to freeze brain slices for immuno. The slices are 250uM cut with a vibratome. I have been trying to embed these slices in OCT so that I may section them using the cryostat. It seems as though the tissue is not freezing flat. Does anyone have any suggestions? I cannot get the slices to freeze flat no matter what i try. Thank you! Stacey barrickstacey@yahoo.com --------------------------------- Sponsored Link Mortgage rates near 39yr lows. $510,000 Mortgage for $1,698/mo - Calculate new house payment From AnthonyH <@t> chw.edu.au Thu Nov 16 15:15:34 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Nov 16 15:17:24 2006 Subject: [Histonet] Re: Alcian Yellow & Alcian Blue 8GX Message-ID: My wife has a used husband for sale. Cheap, some mileage, tends to be unreliable at times. A renovators challenge!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: Thursday, 16 November 2006 7:20 PM To: Sinoera Tech; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Alcian Yellow & Alcian Blue 8GX I've got a bike for sale, 6 back gears, 3 front with a padded seat, ?200 plus packaging and postage. Please contact me on the below number if you want a test drive. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net A belief is purely an individual matter, and you cannot and must not organize it. --J. Krishnamurti This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From Chariza_Reyes-Espidol <@t> hchd.tmc.edu Thu Nov 16 16:03:44 2006 From: Chariza_Reyes-Espidol <@t> hchd.tmc.edu (Reyes-Espidol, Chariza M) Date: Thu Nov 16 16:03:55 2006 Subject: [Histonet] Remove the paraffin from clothing! Message-ID: <1872B4A455B7974391609AD8034C79FC0A56F4@LBEXCH01.hchd.local> Hello everyone, Does anybody know the best way to remove paraffin from clothing? Thank in advance, Chariza CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From naje1972 <@t> yahoo.com Thu Nov 16 16:22:39 2006 From: naje1972 <@t> yahoo.com (cynthia haynes) Date: Thu Nov 16 16:22:45 2006 Subject: [Histonet] 40 microns Message-ID: <115624.66838.qm@web33009.mail.mud.yahoo.com> One of the researchers that works in the Immunology dept is wanting to stain 40 micron paraffin embedded formalin fixed brain tissue with alpha- syn nuclein and gfap. Her question is how long should she deparaffinize her slides. If you have a protocol that you would be willing to share that would be greatly appreciated. Thank you in advance. Cynthia Haynes H.T. ____________________________________________________________________________________ Sponsored Link Online degrees - find the right program to advance your career. Www.nextag.com From stamptrain <@t> yahoo.com Thu Nov 16 15:34:11 2006 From: stamptrain <@t> yahoo.com (Roger Moretz) Date: Thu Nov 16 16:34:22 2006 Subject: [Histonet] Mineral deposits and von Kossa & alizarin S Message-ID: <667328.73215.qm@web50311.mail.yahoo.com> A colleague has asked me to post this for him: We recently found what appears to be mineral within mouse heart tissue. This mineral stains with von Kossa, but was negative with Alizarin Red S, with the exception of a single mineral deposit in one animal. The hearts were fixed in neutral buffered formalin (NBF). Is it possible that the calcium leached out in the NBF and that is the reason for the negative result with Alizarin Red? Some of the older literature suggests using alcohol fixatives, but more recent literature recommends using NBF. If the calcium leached out would you still expect to see non-staining mineral deposits, or should the entire deposit be dissolved away and absent from the tissue section? Also, some people have suggested that to increase the confidence (of the von Kossa stain) that the blackened material is calcium a duplicate tissue section can be decalcified with citrate buffer (pH 4.5), 10 % formic acid, or 0.5% aqueous hydrochloric acid. Has anybody had any luck with one of these procedures to help characterize mineral? Is there a reference available on this modified method? Regards, Mike Mike Thibodeau, DVM, PhD, DACVP Senior Scientist, Pathologist Department of Toxicology and Safety Assessment Boehringer Ingelheim Pharmaceuticals Inc. 900 Ridgebury Rd, P.O. Box 368 Ridgefield CT, 06877 Phone: (203) 791-6124 Mobile: (203) 546-0260 Email: mthibode@rdg.boehringer-ingelheim.com Roger Moretz, Ph.D. Senior Scientist, Electron Microscopist Dept of Toxicology & Safety Assessment Boehringer Ingelheim Pharmaceuticals, Inc. 900 Ridgebury Rd Ridgefield, CT 06877 ____________________________________________________________________________________ Sponsored Link Mortgage rates near 39yr lows. $420k for $1,399/mo. Calculate new payment! www.LowerMyBills.com/lre From cwscouten <@t> myneurolab.com Thu Nov 16 17:01:38 2006 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Thu Nov 16 17:01:04 2006 Subject: [Histonet] freezing brain slices Message-ID: <5784D843593D874C93E9BADCB87342AB0130788E@tpiserver03.Coretech-holdings.com> Laying them on a cold (but not below freezing) flat pedestal and snap freezing them under powdered dry ice should make them lie flat. How fast are you getting them frozen? The OCT may be absorbing cold and slowing the rate of freezing. You don't need it. Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stacey Barrick Sent: Thursday, November 16, 2006 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] freezing brain slices Hello.. I'm trying to freeze brain slices for immuno. The slices are 250uM cut with a vibratome. I have been trying to embed these slices in OCT so that I may section them using the cryostat. It seems as though the tissue is not freezing flat. Does anyone have any suggestions? I cannot get the slices to freeze flat no matter what i try. Thank you! Stacey barrickstacey@yahoo.com --------------------------------- Sponsored Link $420,000 Mortgage for $1,399/month - Think You Pay Too Much For Your Mortgage? Find Out! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Thu Nov 16 17:05:08 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Nov 16 17:06:51 2006 Subject: [Histonet] Remove the paraffin from clothing! Message-ID: Take some tissue paper or other such absorbent paper. Place it on the wax stain. Use a hot iron and place it on the paper. The wax will absorb to the paper. I usually do this two-three times with fresh paper. You can then dab with some xylene or laundry spray and wash as usual. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Reyes-Espidol, Chariza M Sent: Friday, 17 November 2006 9:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Remove the paraffin from clothing! Hello everyone, Does anybody know the best way to remove paraffin from clothing? Thank in advance, Chariza CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From JWEEMS <@t> sjha.org Thu Nov 16 17:10:24 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Thu Nov 16 17:10:44 2006 Subject: [Histonet] Remove the paraffin from clothing! Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEF287@sjhaexc02.sjha.org> A brown paper grocery bag is the best for this little chore. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Tony Henwood Sent: Thursday, November 16, 2006 6:05 PM To: Reyes-Espidol, Chariza M; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Remove the paraffin from clothing! Take some tissue paper or other such absorbent paper. Place it on the wax stain. Use a hot iron and place it on the paper. The wax will absorb to the paper. I usually do this two-three times with fresh paper. You can then dab with some xylene or laundry spray and wash as usual. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Reyes-Espidol, Chariza M Sent: Friday, 17 November 2006 9:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Remove the paraffin from clothing! Hello everyone, Does anybody know the best way to remove paraffin from clothing? Thank in advance, Chariza CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Nov 17 02:43:01 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Nov 17 02:42:04 2006 Subject: [Histonet] Mineral deposits and von Kossa & alizarin S Message-ID: <86ADE4EB583CE64799A9924684A0FBBFAFC9A4@wahtntex2.waht.swest.nhs.uk> We recently found what appears to be mineral within mouse heart tissue. This mineral stains with von Kossa, but was negative with Alizarin Red S, with the exception of a single mineral deposit in one animal. The hearts were fixed in neutral buffered formalin (NBF). Is it possible that the calcium leached out in the NBF and that is the reason for the negative result with Alizarin Red? Some of the older literature suggests using alcohol fixatives, but more recent literature recommends using NBF. If the calcium leached out would you still expect to see non-staining mineral deposits, or should the entire deposit be dissolved away and absent from the tissue section? Roger I am not familiar with the Alizarin Red stain for bone and thought it was used to demonstrate sites undergoing mineralization. For example madder (a herb containing Alizarin) fed to cattle coloured their bones. There is a technique for using Alizarin Red S to colour the mineralised bones of fetal or newborn rats involving maceration with KOH and Alizarin Red over many days. So I thought you used it specifically to demonstrate developing bone. Von Kossa's, on the other hand, like Lillie's method of silver impregnation, demonstrates phosphates rather than calcium salts, but as soluble phosphates are washed out it is essentially calcium phosphate that is demonstrated. There are a variety of techniques available to demonstrate calcium (phosphate) and Lillie proposes a block technique after formol fixation; 1) Immerse blocks in 6 changes of dist water for 20 min each. 2) Place blocks in 1.7% (0.1M) silver nitrate and keep in dark. 3) Remove blocks between 5 days and 10 days (experiment). 4) Wash in 6 changes of dist water for 20 min each. 5) Transfer to 2g sodium bromide in 94 ml dist water for 3 hours. 6) Add 6 ml glacial acetic acid. 7) This mixture (2% sodium bromide- 6 % glacial acetic acid decalcifying fluid is changed daily. 1 ml discarded fluid is tested daily with 1 ml 2% sodium oxalate until there is no turbidity. 8) You can the process the block, cut sections, stain with collagen or elastin stains. Calcified areas are black. Brought to you from the disciple of that great man Prof R.D. Lillie MD. PS Alcohol fixation is 'said' to be better. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net A belief is purely an individual matter, and you cannot and must not organize it. --J. Krishnamurti This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Nov 17 02:47:22 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Nov 17 02:46:24 2006 Subject: [Histonet] 40 microns Message-ID: <86ADE4EB583CE64799A9924684A0FBBFAFC9A6@wahtntex2.waht.swest.nhs.uk> Its trite answer but until the paraffin has gone. Used to cut thick brain sections on a Tetrander and I used to deparafinise sections for maybe 10 min. I don't think you can over deparafinise. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net A belief is purely an individual matter, and you cannot and must not organize it. --J. Krishnamurti This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Nov 17 02:48:11 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Nov 17 02:47:14 2006 Subject: [Histonet] Remove the paraffin from clothing! Message-ID: <86ADE4EB583CE64799A9924684A0FBBFAFC9A7@wahtntex2.waht.swest.nhs.uk> Hello everyone, Does anybody know the best way to remove paraffin from clothing? Thank in advance, Chariza Set fire to it? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net A belief is purely an individual matter, and you cannot and must not organize it. --J. Krishnamurti This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Nov 17 02:51:57 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Nov 17 02:51:00 2006 Subject: [Histonet] freezing brain slices Message-ID: <86ADE4EB583CE64799A9924684A0FBBFAFC9A8@wahtntex2.waht.swest.nhs.uk> Buy a tongue press? (A press for pressing ox tongue after cooking) Then press the brain lightly and freeze the whole thing (may need to put paper between brain and press). I bet you could make frozen flat anything if you did that. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net A belief is purely an individual matter, and you cannot and must not organize it. --J. Krishnamurti This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From David.Muskett <@t> RLC.NHS.UK Fri Nov 17 03:07:05 2006 From: David.Muskett <@t> RLC.NHS.UK (Muskett David) Date: Fri Nov 17 03:07:08 2006 Subject: [Histonet] TUNEL methods Message-ID: Dear All We are starting a project using tunel and the kit insert leaflet we have from Roche is not very clear. Does anyone have a tunel method (with buffers required) that they would be willing to share. Thanks David David Muskett Chief Biomedical Scientist, Histology Royal Liverpool Children's NHS Trust Eaton Road Liverpool L12 2AP Internal telephone x3615 Telephone (0151) 293 3656 Fax (0151) 293 3617 http://rlcnet/Histopathology/ http://www.alderhey.com/RLCH/Laboratory_Medicine.asp From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Nov 17 04:03:47 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Nov 17 04:02:55 2006 Subject: [Histonet] Re: Alcian Yellow & Alcian Blue 8GX Message-ID: <86ADE4EB583CE64799A9924684A0FBBFAFC9A9@wahtntex2.waht.swest.nhs.uk> My wife has a used husband for sale. Cheap, some mileage, tends to be unreliable at times. A renovators challenge!! Regards Got someone interested in your wife's used husband. But she needs to know some details; 1) Has he got his own teeth? 2) Is he continent? 3) Are there any special washing instructions? 4) Is there any manufacturers guarantee? 5) Is he house trained? 6) Has he had all his shots? I'm sure if you have reasonable answers then we can broker a deal; how much were you thinking of? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net A belief is purely an individual matter, and you cannot and must not organize it. --J. Krishnamurti This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From petepath <@t> yahoo.com Fri Nov 17 07:00:35 2006 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Fri Nov 17 07:00:45 2006 Subject: [Histonet] freezing brain slices Message-ID: <20061117130035.6598.qmail@web30404.mail.mud.yahoo.com> Dear Stacy, I believe you can accomplish this task using my paper embedding technique. If you finish the block with cold embedding medium you will minimize freeze artifact. The technique is desribed at: http://pathologyinnovations.com/Paper%20Embedding.htm. Stephen Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com From oshel1pe <@t> cmich.edu Fri Nov 17 07:08:24 2006 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Fri Nov 17 07:08:43 2006 Subject: [Histonet] Mineral deposits and von Kossa & alizarin S In-Reply-To: <667328.73215.qm@web50311.mail.yahoo.com> References: <667328.73215.qm@web50311.mail.yahoo.com> Message-ID: Do you have access to a SEM with an energy-dispersive x-ray spectrometer (EDS)? If yes, this could give a positive ID of the element(s) and would be relatively quick. If you only care about the mineral and not morphology, at least initially, you can air-dry unfixed tissue, mount, and carbon-coat to do the spectra. Phil >A colleague has asked me to post this for him: > >We recently found what appears to be mineral within >mouse heart tissue. This mineral stains with von >Kossa, but was negative with Alizarin Red S, with the >exception of a single mineral deposit in one animal. >The hearts were fixed in neutral buffered formalin >(NBF). Is it possible that the calcium leached out in >the NBF and that is the reason for the negative result >with Alizarin Red? Some of the older literature >suggests using alcohol fixatives, but more recent >literature recommends using NBF. If the calcium >leached out would you still expect to see non-staining >mineral deposits, or should the entire deposit be >dissolved away and absent from the tissue section? > >Also, some people have suggested that to increase the >confidence (of the von Kossa stain) that the blackened >material is calcium a duplicate tissue section can be >decalcified with citrate buffer (pH 4.5), 10 % formic >acid, or 0.5% aqueous hydrochloric acid. Has anybody >had any luck with one of these procedures to help >characterize mineral? Is there a reference available >on this modified method? > > >Regards, >Mike > >Mike Thibodeau, DVM, PhD, DACVP >Senior Scientist, Pathologist >Department of Toxicology and Safety Assessment > >Boehringer Ingelheim Pharmaceuticals Inc. >900 Ridgebury Rd, P.O. Box 368 >Ridgefield CT, 06877 >Phone: (203) 791-6124 >Mobile: (203) 546-0260 >Email: mthibode@rdg.boehringer-ingelheim.com > >Roger Moretz, Ph.D. >Senior Scientist, Electron Microscopist >Dept of Toxicology & Safety Assessment >Boehringer Ingelheim Pharmaceuticals, Inc. >900 Ridgebury Rd >Ridgefield, CT 06877 > > > > > >____________________________________________________________________________________ >Sponsored Link > >Mortgage rates near 39yr lows. >$420k for $1,399/mo. Calculate new payment! >www.LowerMyBills.com/lre > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Philip Oshel Microscopy Facility Supervisor Biology Department Central Michigan University 024C Brooks Hall Mt. Pleasant, MI 48859 voice: (989) 774-3576 dept. fax: (989) 774-3462 From jmyers1 <@t> aol.com Fri Nov 17 07:26:36 2006 From: jmyers1 <@t> aol.com (jmyers1@aol.com) Date: Fri Nov 17 07:26:49 2006 Subject: [Histonet] Eridan news? Message-ID: <8C8D86324156D7C-2B8-4A00@webmail-da12.sysops.aol.com> Unless I've somehow missed seeing it, I was just wondering why we haven't seen (any) feedback on this inquiry from a week ago today? I'm very interested in learning about the status of this system... ------------------------------ Message: 20 Date: Fri, 10 Nov 2006 18:29:18 +1300 From: "Alan Bishop" Subject: [Histonet] Eridan news? To: histonet@lists.utsouthwestern.edu Anyone heard any further news on the Dako Eridan? Have heard today that the machines that were in labs have been withdrawn but not been able to get confirmation of this? Looks like it might be the end of the orad for the mythical new machine. Anyone heard anything?? Cheers A ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From DDittus787 <@t> aol.com Fri Nov 17 07:47:12 2006 From: DDittus787 <@t> aol.com (DDittus787@aol.com) Date: Fri Nov 17 07:47:37 2006 Subject: [Histonet] Remove the paraffin from clothing! Message-ID: <576.9f3c105.328f1760@aol.com> I was told, yet have not had to do this,but place the clothing between two brown paper bags(do they still have these?) and use a hot iron-supposedly it melts the paraffin and the bags absorbs the grease. Good luck. dana From dfvilleg <@t> mtu.edu Fri Nov 17 07:50:52 2006 From: dfvilleg <@t> mtu.edu (Diego Villegas) Date: Fri Nov 17 07:51:03 2006 Subject: [Histonet] calcium in the enthesis ligament Message-ID: <455DBE3C.8020307@mtu.edu> Hi all, I am working with bovine ligaments, and I have to identify the calcium present on the knee ligament enthesis. I would like to know if Alizarin red S is the best to this and, the protocol suggest a pH solution of 4.1 - 4.3. The stain solution of our supplier has a pH ranged between 4.5 to 5. Could I work with this stain solution?. Thanks, Diego Villegas Biomechanics Lab Michigan Tech Uninversity From Barry.R.Rittman <@t> uth.tmc.edu Fri Nov 17 08:25:54 2006 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Fri Nov 17 08:26:01 2006 Subject: [Histonet] TSH Convention Hotel Message-ID: Texas Society for Histotechnology "DIAMOND DAYS OF HISTOLOGY" TSH 2007 ANNUAL SYMPOSIUM APRIL 19-22, 2007 RENAISSANCE HOUSTON HOTEL GREENWAY PLAZA 6 GREENWAY PLAZA EAST HOUSTON, TEXAS 77046 713-629-1200 From ree3 <@t> leicester.ac.uk Fri Nov 17 08:36:05 2006 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri Nov 17 08:36:17 2006 Subject: [Histonet] Eridan news? In-Reply-To: <8C8D86324156D7C-2B8-4A00@webmail-da12.sysops.aol.com> Message-ID: Well the green dragon was defeated and the elves and dwarfs divided its treasure hoard and lived happily ever after in the smokey hollow beneath the snowy mountain.. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jmyers1@aol.com Sent: 17 November 2006 13:27 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eridan news? Unless I've somehow missed seeing it, I was just wondering why we haven't seen (any) feedback on this inquiry from a week ago today? I'm very interested in learning about the status of this system... ------------------------------ Message: 20 Date: Fri, 10 Nov 2006 18:29:18 +1300 From: "Alan Bishop" Subject: [Histonet] Eridan news? To: histonet@lists.utsouthwestern.edu Anyone heard any further news on the Dako Eridan? Have heard today that the machines that were in labs have been withdrawn but not been able to get confirmation of this? Looks like it might be the end of the orad for the mythical new machine. Anyone heard anything?? Cheers A ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Fri Nov 17 08:48:43 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Fri Nov 17 09:01:24 2006 Subject: [Histonet] Eridan news? Message-ID: Well thank goodness for that! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: Friday, November 17, 2006 9:36 AM To: jmyers1@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Eridan news? Well the green dragon was defeated and the elves and dwarfs divided its treasure hoard and lived happily ever after in the smokey hollow beneath the snowy mountain.. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jmyers1@aol.com Sent: 17 November 2006 13:27 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eridan news? Unless I've somehow missed seeing it, I was just wondering why we haven't seen (any) feedback on this inquiry from a week ago today? I'm very interested in learning about the status of this system... ------------------------------ Message: 20 Date: Fri, 10 Nov 2006 18:29:18 +1300 From: "Alan Bishop" Subject: [Histonet] Eridan news? To: histonet@lists.utsouthwestern.edu Anyone heard any further news on the Dako Eridan? Have heard today that the machines that were in labs have been withdrawn but not been able to get confirmation of this? Looks like it might be the end of the orad for the mythical new machine. Anyone heard anything?? Cheers A ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tes4 <@t> u.washington.edu Fri Nov 17 09:22:24 2006 From: tes4 <@t> u.washington.edu (Tiffany Pitts) Date: Fri Nov 17 09:17:04 2006 Subject: [Histonet] Remove the paraffin from clothing! References: <576.9f3c105.328f1760@aol.com> Message-ID: <003a01c70a5c$2f55f560$4b00a8c0@gucancer.local> This actually does work, I've tried it. I have heard that you can put a piece of gauze (or an old, thinning kitchen towl) between the paper and the cloth to help to wick up the hot parrafin as well, but I haven't tried this yet. Good luck Tiffany ----- Original Message ----- From: To: ; ; Sent: Friday, November 17, 2006 5:47 AM Subject: Re: [Histonet] Remove the paraffin from clothing! >I was told, yet have not had to do this,but place the clothing between two > brown paper bags(do they still have these?) and use a hot iron-supposedly > it > melts the paraffin and the bags absorbs the grease. Good luck. > > > dana > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JWEEMS <@t> sjha.org Fri Nov 17 09:20:10 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Nov 17 09:20:29 2006 Subject: [Histonet] Eridan news? Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEF2A1@sjhaexc02.sjha.org> I am so glad to know there is a happily ever after! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Edwards, R.E. Sent: Friday, November 17, 2006 9:36 AM To: jmyers1@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Eridan news? Well the green dragon was defeated and the elves and dwarfs divided its treasure hoard and lived happily ever after in the smokey hollow beneath the snowy mountain.. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jmyers1@aol.com Sent: 17 November 2006 13:27 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eridan news? Unless I've somehow missed seeing it, I was just wondering why we haven't seen (any) feedback on this inquiry from a week ago today? I'm very interested in learning about the status of this system... ------------------------------ Message: 20 Date: Fri, 10 Nov 2006 18:29:18 +1300 From: "Alan Bishop" Subject: [Histonet] Eridan news? To: histonet@lists.utsouthwestern.edu Anyone heard any further news on the Dako Eridan? Have heard today that the machines that were in labs have been withdrawn but not been able to get confirmation of this? Looks like it might be the end of the orad for the mythical new machine. Anyone heard anything?? Cheers A ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From Jessica <@t> medstaffservices.com Fri Nov 17 09:34:02 2006 From: Jessica <@t> medstaffservices.com (Jessica Hirsch) Date: Fri Nov 17 09:34:17 2006 Subject: [Histonet] Please post message Message-ID: CURRENT EMPLOYMENT OPPROTUNITIES (HISTOLOGY, IMMUNOHISTOCHEMISTRY & PATHOLOGY) For immediate consideration for any position: Send resumes to Jessica@medstaffservices.com OR fax to 732.238.2152 For questions call: Jessica Hirsch at 732.238.6050 ext.58 I. Clarksburg, Maryland (Large Nationwide Clinical Labratory (division of BioReference)) 1. Histology Supervisor: Hours: Monday - Friday 12 midnight - 8:30am Pay: ~ $30/hr Benefits: Medical, Dental, 401k 2. Histology Technologist Hours: Monday - Friday 12 midnight - 8:30am Pay: $22/hr + 10% night differential = $24.20/hr Benefits: Medical, Dental, 401k 3. Grossers Hours: Monday - Friday 7pm-11:30pm Pay: $30/hr II. Elmwood Park, New Jersey ( Nationwide Clinical Laboratory) 1. Histology Technologist ( 2 positions available, immediate start ) Hours: Monday - Friday, 9:00pm - 5:30am Pay: Negotiable Benefits: Medical, Dental, 401k 2. Cytogenetic Supervisor Hours: Monday - Friday, 8am - 5pm Pay: Negotiable Benefits: Medical, Dental, 401k III. New York City, New York ( large world renowned Cancer Hospital ) 1. Pathologist Assistant Hours: Monday - Friday, Day shift, Full-Time Pay: $60k - $80k based on experience Benefits: Major Medical, Dental, Vision, 401k, etc. 2. Histology Technologist Hours: Monday - Friday (shift to be announced) Pay: negotiable Benefits: Medical, Dental, 401k For immediate consideration for any position: Send resumes to Jessica@medstaffservices.com OR fax to 732.238.2152 For questions call: Jessica Hirsch at 732.238.6050 ext.58 Jessica D. Hirsch Staffing Specialist Medical Staffing Services, Inc. 557 Cranbury Road East Brunswick, NJ 08816 P: (732) 238-6050 x58 F: (732) 238-2152 Jessica@medstaffservices.com http://www.medstaffservices.com From MSHERWOOD <@t> PARTNERS.ORG Fri Nov 17 09:39:12 2006 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Fri Nov 17 09:39:22 2006 Subject: [Histonet] TUNEL methods In-Reply-To: Message-ID: <1AF23D0AD12E7444A5DB083CA978B73407A30D8E@PHSXMB1.partners.org> I would be interested in the methods also. Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Muskett David Sent: Friday, November 17, 2006 4:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] TUNEL methods Dear All We are starting a project using tunel and the kit insert leaflet we have from Roche is not very clear. Does anyone have a tunel method (with buffers required) that they would be willing to share. Thanks David David Muskett Chief Biomedical Scientist, Histology Royal Liverpool Children's NHS Trust Eaton Road Liverpool L12 2AP Internal telephone x3615 Telephone (0151) 293 3656 Fax (0151) 293 3617 http://rlcnet/Histopathology/ http://www.alderhey.com/RLCH/Laboratory_Medicine.asp From MadaryJ <@t> MedImmune.com Fri Nov 17 09:47:39 2006 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Fri Nov 17 09:48:15 2006 Subject: [Histonet] HTL comments Message-ID: <7CAB706201F11843BD26AD516326F0C801DDB724@MD1MS007.medimmune.com> I took the HTL about 5 yrs ago, and a friend took it last year and we both had the same recommendations for the HTL which is the Bancroft and Stevens. I read it twice, and I passed with a much higher score than I imagined, in the 900's(go ahead Nocito say something). Admittedly I took it after 23 yrs in the field, and that time in the lab is really what got me there. The reading material was more of a refresher on what to expect. That is a great book, so is Kiernans. I have always thought that HTL exam should be reserved for folks with at least 10 yrs in the field, just so they can have a decent chance at passing via their experience and given an amount of respect for time served. We have already beaten to tdeath the HT.HTL.QIHC folks who cannot perform. I knew a guy with an HTL who said he only cut for his exam, he wanted the HTL for his business cards because he was a sales rep. He was good at IHC though. Anyhistow, I am sorry the girls had a bad experience. I hope they go back and take it again. Nick Madary, HT/HTL(ASCP)QIHC Histology Mgr, Medimmune One Medimmune Way Gaithersburg, MD 20878 301.398.6113/4745/(Fax9745) From gcallis <@t> montana.edu Fri Nov 17 10:20:10 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Nov 17 10:20:27 2006 Subject: [Histonet] HTL comments In-Reply-To: <7CAB706201F11843BD26AD516326F0C801DDB724@MD1MS007.medimmun e.com> References: <7CAB706201F11843BD26AD516326F0C801DDB724@MD1MS007.medimmune.com> Message-ID: <6.0.0.22.1.20061117091207.01ba7e80@gemini.msu.montana.edu> The Bancroft and Stevens book has been updated with a newer 5th edition in 2001 by Gamble and Bancroft, under the same title, Theory and Practice of Histological Techniques. There is an extensive immunohistochemistry chapter and also a immunofluorescence chapter. The freebie manual from DAKO in pdf form on their website is a good learning book too. Sheehan and Hrapchaks book may have some good BASIC information, but it is really outdated (26 years old) considering what we do today for immunostaining procedures. As for other routine stains for paraffin sections, it is still one of the best! Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 At 08:47 AM 11/17/2006, you wrote: >I took the HTL about 5 yrs ago, and a friend took it last year and we >both had the same recommendations for the HTL which is the Bancroft and >Stevens. I read it twice, and I passed with a much higher score than I >imagined, in the 900's(go ahead Nocito say something). Admittedly I >took it after 23 yrs in the field, and that time in the lab is really >what got me there. The reading material was more of a refresher on what >to expect. That is a great book, so is Kiernans. I have always thought >that HTL exam should be reserved for folks with at least 10 yrs in the >field, just so they can have a decent chance at passing via their >experience and given an amount of respect for time served. We have >already beaten to tdeath the HT.HTL.QIHC folks who cannot perform. I >knew a guy with an HTL who said he only cut for his exam, he wanted the >HTL for his business cards because he was a sales rep. He was good at >IHC though. Anyhistow, I am sorry the girls had a bad experience. I >hope they go back and take it again. > >Nick Madary, HT/HTL(ASCP)QIHC >Histology Mgr, Medimmune >One Medimmune Way >Gaithersburg, MD 20878 > >301.398.6113/4745/(Fax9745) >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Fri Nov 17 10:43:31 2006 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Fri Nov 17 10:43:57 2006 Subject: [Histonet] Remove the paraffin from clothing! In-Reply-To: <003a01c70a5c$2f55f560$4b00a8c0@gucancer.local> Message-ID: <200611171643.kAHGhfpn070460@pro12.abac.com> I put the clothing between two paper towels, iron and the towels absorbs the paraffin, works on carpet two even with one towel on top, for when candle wax is spilled. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tiffany Pitts Sent: Friday, November 17, 2006 8:22 AM To: Chariza_Reyes-Espidol@hchd.tmc.edu; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Remove the paraffin from clothing! This actually does work, I've tried it. I have heard that you can put a piece of gauze (or an old, thinning kitchen towl) between the paper and the cloth to help to wick up the hot parrafin as well, but I haven't tried this yet. Good luck Tiffany ----- Original Message ----- From: To: ; ; Sent: Friday, November 17, 2006 5:47 AM Subject: Re: [Histonet] Remove the paraffin from clothing! >I was told, yet have not had to do this,but place the clothing between two > brown paper bags(do they still have these?) and use a hot iron-supposedly > it > melts the paraffin and the bags absorbs the grease. Good luck. > > > dana > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Fri Nov 17 10:57:50 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Nov 17 10:58:01 2006 Subject: [Histonet] freezing brain slices Message-ID: <4EBFF65383B74D49995298C4976D1D5E273BB1@LSRIEXCH1.lsmaster.lifespan.org> I have worked out a method for freezing thin tissues flat, that works well for me. I keep two stainless steel plates in the -80 freezer. These are actually stainless steel covers for staining dishes. I take a thin piece of polyethylene, about 3x3", cut from a plastic bag. Not a sandwich bag, a good quality heavy duty plastic bag like you might use for storing specimens. I put a bit of OCT on the plastic, place the specimen on it, add another drop of OCT on top, then place a second plastic square on top. With gentle pressure from a tongue depressor or similar flat object, I flatten and smooth the OCT and tissue between the two pieces of plastic. Then I take it to the freezer. I drop the plastic/OCT/tissue "sandwich" onto one of the pre-chilled steel plates, then quickly - I emphasise quickly! - place the other steel plate on top. The tissue is flattened, and freezes in a few seconds. If the tissue is soft, and I think the weight of the steel plate might crush or compress it too much, I place a microscope slide or similar spacer on each end of the bottom plate, place the tissue between them and quickly put the second plate on top. That way the OCT and tissue can't be compressed any more than the thickness of the spacer. I then remove the OCT/tissue wafer from between the layers of plastic, keeping it in the freezer. To mount it for sectioning without thawing, I lay the wafer on one of the steel plates, then take a frozen blank OCT block (frozen in the standard freezer, not the -80), soften the surface a bit with my finger, apply a bit of additional OCT to the face of the block, then before it can freeze, press it down onto the wafer on the cold plate. From pruegg <@t> ihctech.net Fri Nov 17 11:35:22 2006 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Fri Nov 17 11:35:56 2006 Subject: [Histonet] freezing brain slices In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E273BB1@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <200611171735.kAHHZXZk000642@pro12.abac.com> For Mohs we always froze the tissue on a glass slide so that it would be flat, just lay the tissue on a slide that is in the cryostat, add OCT and allow to freeze quickly. Once frozen, a touch with the thumb on the slide will allow the tissue to be released and it can then be attached to a cryostat chuck with the flat slide side up. The surface comes out really flat. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Friday, November 17, 2006 9:58 AM To: 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] freezing brain slices I have worked out a method for freezing thin tissues flat, that works well for me. I keep two stainless steel plates in the -80 freezer. These are actually stainless steel covers for staining dishes. I take a thin piece of polyethylene, about 3x3", cut from a plastic bag. Not a sandwich bag, a good quality heavy duty plastic bag like you might use for storing specimens. I put a bit of OCT on the plastic, place the specimen on it, add another drop of OCT on top, then place a second plastic square on top. With gentle pressure from a tongue depressor or similar flat object, I flatten and smooth the OCT and tissue between the two pieces of plastic. Then I take it to the freezer. I drop the plastic/OCT/tissue "sandwich" onto one of the pre-chilled steel plates, then quickly - I emphasise quickly! - place the other steel plate on top. The tissue is flattened, and freezes in a few seconds. If the tissue is soft, and I think the weight of the steel plate might crush or compress it too much, I place a microscope slide or similar spacer on each end of the bottom plate, place the tissue between them and quickly put the second plate on top. That way the OCT and tissue can't be compressed any more than the thickness of the spacer. I then remove the OCT/tissue wafer from between the layers of plastic, keeping it in the freezer. To mount it for sectioning without thawing, I lay the wafer on one of the steel plates, then take a frozen blank OCT block (frozen in the standard freezer, not the -80), soften the surface a bit with my finger, apply a bit of additional OCT to the face of the block, then before it can freeze, press it down onto the wafer on the cold plate. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carolb <@t> phys.mcw.edu Fri Nov 17 12:18:01 2006 From: carolb <@t> phys.mcw.edu (Bobrowitz, Carol) Date: Fri Nov 17 12:18:06 2006 Subject: [Histonet] EnVision Kits Message-ID: <8F78639AC56F4143B267FE5F5A1B92C80E43B1@guyton.phys.mcw.edu> Hi, I have a researcher who wants to do ER staining using the EnVision Plus HRP system. She will be using a monoclonal and a polyclonal primary antibody. I have read the EnVision specification sheet and understand its made in goat. I have been told that staining on human breast tissue is very good. My question: Is the EnVision system compatible with RAT Tissue, breast tumors? Years ago I had difficulty getting good IHC staining on rat tissue using EnVision. I switched to using the Rat Kit and had much better results. Any comments or help will be greatly appreciated. Thank you in advance. Carol Ann Bobrowitz Medical College of Wisconsin Physiology Department cbobrowi@mcw.edu From mcauliff <@t> umdnj.edu Fri Nov 17 13:42:29 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Nov 17 13:41:54 2006 Subject: [Histonet] disposable knife holder needed Message-ID: <455E10A5.6060306@umdnj.edu> Dear List: I need a disposable knife holder for a Reichert-Jung Histocut rotary microtome. Leica no longer carries this part and standard dispo. knife holders do not fit in the factory knife holder. Any help would be appreciated. Geoff -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From liz <@t> premierlab.com Fri Nov 17 14:01:53 2006 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Nov 17 13:53:44 2006 Subject: [Histonet] black wax in dissecting pans Message-ID: <000001c70a83$3a15a8c0$0300a8c0@domain.Premier> Hello All Does anyone know were I can get the black wax that is in used in the metal dissecting pans. I know that I can order the pans with the wax, but I was wondering if anyone out there knows if I can just purchase the wax alone. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 From Eric.C.Kellar <@t> questdiagnostics.com Fri Nov 17 14:14:17 2006 From: Eric.C.Kellar <@t> questdiagnostics.com (Kellar, Eric C) Date: Fri Nov 17 14:14:38 2006 Subject: [Histonet] black wax in dissecting pans Message-ID: <6843061CE6B98E4B96590D4F299618F8015666E3@qdcws0117.us.qdx.com> Liz, I've ordered mine from http://www.enasco.com . About $5.40 per lb TGIF! Eric C. Kellar Quest Diagnostics, Inc Miami -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Liz Chlipala Sent: Friday, November 17, 2006 3:02 PM To: 'Histonet' Subject: [Histonet] black wax in dissecting pans Hello All Does anyone know were I can get the black wax that is in used in the metal dissecting pans. I know that I can order the pans with the wax, but I was wondering if anyone out there knows if I can just purchase the wax alone. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ============================================================================== The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. ============================================================================== From josephnerk <@t> hotmail.com Fri Nov 17 14:16:57 2006 From: josephnerk <@t> hotmail.com (Joseph Nerk) Date: Fri Nov 17 14:17:12 2006 Subject: [Histonet] 10 % Formalin Message-ID: Can anyone in histo-land provide in formation on 10 % formalin GRG grade. I have been having problems with it, checked pH and it was acid. Seen some pigments in the small biopsies. _________________________________________________________________ Express yourself instantly with MSN Messenger! [1]MSN Messenger Download today it's FREE! References 1. http://g.msn.com/8HMBEN/2728??PS=47575 From bwhitaker <@t> brownpathology.com Fri Nov 17 14:46:39 2006 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Fri Nov 17 14:42:36 2006 Subject: [Histonet] black wax in dissecting pans In-Reply-To: <000001c70a83$3a15a8c0$0300a8c0@domain.Premier> Message-ID: <000901c70a89$7b2ad370$3601a8c0@brownpathology.net> Bennie & Smith perhaps : ) No, seriously, get some of the coloring agent from a candle-making supply/craft store. I'd bet that would work to color regular paraffin. Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Friday, November 17, 2006 2:02 PM To: 'Histonet' Subject: [Histonet] black wax in dissecting pans Hello All Does anyone know were I can get the black wax that is in used in the metal dissecting pans. I know that I can order the pans with the wax, but I was wondering if anyone out there knows if I can just purchase the wax alone. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Fri Nov 17 15:01:55 2006 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Nov 17 14:53:56 2006 Subject: [Histonet] black wax in dissecting pans In-Reply-To: <000901c70a89$7b2ad370$3601a8c0@brownpathology.net> Message-ID: <000001c70a8b$9d0b3dc0$0300a8c0@domain.Premier> We thought about that but we are using the wax for pinning "en face" analysis of mouse aorta's and we thought that candle wax would be to brittle and we were uncertain if we could get the black candle wax dark enough for imaging. We are using special pins that we get through fine since tools they are 0.1mm in diameter. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: Bonnie Whitaker [mailto:bwhitaker@brownpathology.com] Sent: Friday, November 17, 2006 1:47 PM To: 'Liz Chlipala'; 'Histonet' Subject: RE: [Histonet] black wax in dissecting pans Bennie & Smith perhaps : ) No, seriously, get some of the coloring agent from a candle-making supply/craft store. I'd bet that would work to color regular paraffin. Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Friday, November 17, 2006 2:02 PM To: 'Histonet' Subject: [Histonet] black wax in dissecting pans Hello All Does anyone know were I can get the black wax that is in used in the metal dissecting pans. I know that I can order the pans with the wax, but I was wondering if anyone out there knows if I can just purchase the wax alone. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1870 (20061117) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From PMonfils <@t> Lifespan.org Fri Nov 17 14:57:57 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Nov 17 14:58:10 2006 Subject: [Histonet] black wax in dissecting pans Message-ID: <4EBFF65383B74D49995298C4976D1D5E273BB2@LSRIEXCH1.lsmaster.lifespan.org> It can be purchased here: http://www.ctvalleybio.com Cheap! $12.50 for 5 pounds! I don't recommend adding colorant to the embedding wax for use in a dissecting pan. The dissecting wax is a different consistency, softer and more flexible. It doesn't chip or crack when you push sharp objects into it. Paul M. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Liz > Chlipala > Sent: Friday, November 17, 2006 12:01 PM > To: 'Histonet' > Subject: [Histonet] black wax in dissecting pans > > Hello All > > > > Does anyone know were I can get the black wax that is in used in the metal > dissecting pans. I know that I can order the pans with the wax, but I was > wondering if anyone out there knows if I can just purchase the wax alone. > > > > Thanks in advance > > > > Liz > > > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > > Manager > > Premier Laboratory, LLC > > P.O. Box 18592 > > Boulder, CO 80308 > > phone (303) 735-5001 > > fax (303) 735-3540 > > liz@premierlab.com > > www.premierlab.com > > > > Ship to Address: > > > > Premier Laboratory, LLC > > University of Colorado at Boulder > > MCDB, Room A3B40 > > Boulder, CO 80309 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From jfish <@t> gladstone.ucsf.edu Fri Nov 17 15:20:36 2006 From: jfish <@t> gladstone.ucsf.edu (Jo Dee Fish) Date: Fri Nov 17 15:20:49 2006 Subject: [Histonet] black wax in dissecting pans In-Reply-To: <000001c70a8b$9d0b3dc0$0300a8c0@domain.Premier> Message-ID: <000001c70a8e$39128fa0$8903010a@JFISH> Liz, I make mine "homemade" starting with 1 lb of pink dental wax (a bit softer than paraffin so it won't split or crack when a pin is stuck in it) and black wax dye, I use about 1/4 oz. per lb. of wax. I use it for exactly what you are doing, pinning out aortas. Good luck, Jo Dee Jo Dee Fish Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Friday, November 17, 2006 1:02 PM To: 'Bonnie Whitaker'; 'Histonet' Subject: RE: [Histonet] black wax in dissecting pans We thought about that but we are using the wax for pinning "en face" analysis of mouse aorta's and we thought that candle wax would be to brittle and we were uncertain if we could get the black candle wax dark enough for imaging. We are using special pins that we get through fine since tools they are 0.1mm in diameter. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: Bonnie Whitaker [mailto:bwhitaker@brownpathology.com] Sent: Friday, November 17, 2006 1:47 PM To: 'Liz Chlipala'; 'Histonet' Subject: RE: [Histonet] black wax in dissecting pans Bennie & Smith perhaps : ) No, seriously, get some of the coloring agent from a candle-making supply/craft store. I'd bet that would work to color regular paraffin. Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Friday, November 17, 2006 2:02 PM To: 'Histonet' Subject: [Histonet] black wax in dissecting pans Hello All Does anyone know were I can get the black wax that is in used in the metal dissecting pans. I know that I can order the pans with the wax, but I was wondering if anyone out there knows if I can just purchase the wax alone. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1870 (20061117) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lorimroberts2 <@t> yahoo.com Fri Nov 17 15:43:41 2006 From: lorimroberts2 <@t> yahoo.com (Lori Roberts) Date: Fri Nov 17 15:43:51 2006 Subject: [Histonet] Do you have a Microm HM 335E user manual? Message-ID: <274656.29589.qm@web34313.mail.mud.yahoo.com> Hi there, I just purchased a used Microm HM 335E microtome, which unfortunately did not include a manual. Microm has not responded to my request for a replacement manual. I would be very grateful if someone could send me a xerox of their manual. In return, I will send you a Starbucks or Barnes & Noble gift card (your choice). Thanks, Lori --------------------------------- Check out the all-new Yahoo! Mail beta - Fire up a more powerful email and get things done faster. From PatPatterson <@t> mhd.com Fri Nov 17 17:13:49 2006 From: PatPatterson <@t> mhd.com (Patterson, Patricia) Date: Fri Nov 17 17:13:58 2006 Subject: [Histonet] Low Yield FNA samples Message-ID: Greetings Histonetters! We have some very low yield FNA's here and we're trying to find a better way to handle them to optimize what our Histology lab needs to deal with. Currently we do a simple spin down with 95% and create a cell block. But sometimes the amount we pull from tube is very small. We've tried a Fibrin clot method using plasma or thrombin - but our method seems time consuming. Someone mentioned an agar cell method or a colloidal bag method but we don't know anyone who is doing them. Any suggestions would be greatly appreciated. Thanks Pat Patterson Methodist Dallas Medical Center patpatterson@mhd.com P - 214-947-3538 F - 214-947-3524 *********************************************************************** This electronic transmission contains information from Methodist Health System and should be considered confidential and privileged. The information contained in the above messages is intended only for the use of the individual(s) and entity(ies) named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution, or use of this information is prohibited. If you receive this transmission in error, please notify the sender immediately by return e-mail. Methodist Health System, its subsidiaries and affiliates hereby claim all applicable privileges related to the transmission of this communication. From mhasu <@t> ottawaheart.ca Fri Nov 17 17:45:49 2006 From: mhasu <@t> ottawaheart.ca (Mirela Hasu) Date: Fri Nov 17 17:46:26 2006 Subject: [Histonet] immunostaining after aquatex mounting Message-ID: Hello, I have few slides with frozen sections that have been already mounted with Aquatex (water based mounting media). I left the slides in water and I was able to remove the cover slip, but the immunostaing procedure that usually works on similar type of frozen sections, not mounted, does not work on these sections. Is there a way to treat these previously mounted frozen sections to work for IHC? Thanks, Mary From dw18 <@t> uchicago.edu Fri Nov 17 18:07:10 2006 From: dw18 <@t> uchicago.edu (David A. Wright) Date: Fri Nov 17 18:07:23 2006 Subject: [Histonet] Re: [freezing rat brain flat on slides] HiDi V36, #23-8 Message-ID: <20061117180710.AGI17508@m4500-03.uchicago.edu> Hi Histonet folks I just wanted to add that, like Patsy, I freeze brain pieces onto glass slides and get surfaces so flat that I hardly lose any tissue when I start cutting - which is nice for stereological calculations. I cut frozen rat brains (divided up in a brain mold) on a sliding microtome. That means I don't have access to the freezing unit of a cryostat, and I put the glass slide (or a square from one) + brain piece on a piece of dry ice in an ice bucket or styrofoam box. When frozen (they are sucrose equilibrated), I put an oval collar of parafilm round the chunk, fill it with OCT and store all the pieces from 1 brain on their slide in a 50ml conical in the freezer till I can cut them. In addition to the flatness, one of the things I like is that I can see through the slide (before I freeze it) that I haven't got any air bubbles in the lateral ventricles (they are hydrocephalic). [Any bubbles shrink so much on freezing it distorts the brain shape.] -David >------------------------------ >Message: 8 >Date: Fri, 17 Nov 2006 10:35:22 -0700 >From: pruegg@ihctech.net >Subject: RE: [Histonet] freezing brain slices For Mohs we always froze the tissue on a glass slide so that it would be flat, just lay the tissue on a slide that is in the cryostat, add OCT and allow to freeze quickly. Once frozen, a touch with the thumb on the slide will allow the tissue to be released and it can then be attached to a cryostat chuck with the flat slide side up. The surface comes out really flat. >Patsy > -------------------------------------- David A. Wright. PhD Section of Neurosurgery University of Chicago From muddymoo <@t> gmail.com Fri Nov 17 21:56:06 2006 From: muddymoo <@t> gmail.com (Alan Bishop) Date: Fri Nov 17 21:56:15 2006 Subject: [Histonet] Eridan news? In-Reply-To: <8C8D86324156D7C-2B8-4A00@webmail-da12.sysops.aol.com> References: <8C8D86324156D7C-2B8-4A00@webmail-da12.sysops.aol.com> Message-ID: I had confirmation from the local agent and offline from a histonetter that the Eridan has been withdrawn from sale due to design fault(s). Apparently it's going to go back to R&D to sort out the problems but not expected to be for sale until at least 2008. From the news floating around on the net I would be surprised if it ever sees light of day again. So all I now need to decide is which of the very tempting offers from Ventana and Vision Biosystems to go for! Automation here I come :-) Cheers Alan On 18/11/06, jmyers1@aol.com wrote: > > Unless I've somehow missed seeing it, I was just wondering why we haven't > seen (any) feedback on this inquiry from a week ago today? I'm very > interested in learning about the status of this system... > > ------------------------------ > > Message: 20 > Date: Fri, 10 Nov 2006 18:29:18 +1300 > From: "Alan Bishop" > > Subject: [Histonet] Eridan news? > To: histonet@lists.utsouthwestern.edu > > Anyone heard any further news on the Dako Eridan? Have heard today that > the > machines that were in labs have been withdrawn but not been able to get > confirmation of this? Looks like it might be the end of the orad for the > mythical new machine. > > Anyone heard anything?? > > Cheers > > A > ------------------------------ > *Check out the new AOL*. > Most comprehensive set of free safety and security tools, free access to > millions of high-quality videos from across the web, free AOL Mail and more. > From ales.kladnik <@t> bf.uni-lj.si Sat Nov 18 02:36:01 2006 From: ales.kladnik <@t> bf.uni-lj.si (Ales Kladnik) Date: Sat Nov 18 02:36:14 2006 Subject: [Histonet] Re: TUNEL methods In-Reply-To: <20061117151752.C4B1536B90@mail.bf.uni-lj.si> References: <20061117151752.C4B1536B90@mail.bf.uni-lj.si> Message-ID: <455EC5F1.4020700@bf.uni-lj.si> we used the Roche kit for TUNEL assay on paraffin embedded plant tissue sections. for the actual tunel reaction you don't need additional buffers, just mix the Labeling and Enzyme solutions. for washing steps we used PBS. i have a pdf-version of our protocol, so please email me if you're interested. -- Ale? Kladnik University of Ljubljana, Biotechnical Faculty, Department of Biology Ve?na pot 111, SI-1000 Ljubljana, Slovenia tel: +386 1 4233388, fax: +386 1 2573390 url: http://botanika.biologija.org/ skype: fridjo, msn: aleskladnik@hotmail.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Muskett David Sent: Friday, November 17, 2006 4:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] TUNEL methods Dear All We are starting a project using tunel and the kit insert leaflet we have from Roche is not very clear. Does anyone have a tunel method (with buffers required) that they would be willing to share. Thanks David From sheila_adey <@t> hotmail.com Sat Nov 18 08:28:45 2006 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Sat Nov 18 08:28:59 2006 Subject: [Histonet] Low Yield FNA samples In-Reply-To: Message-ID: Hi We also were experiencing the same situation. We use to use the Thermo Shandon cysto spin chamber but we found that some of the material would still be in the funnel. So... actually by mistake one day I noticed that their 2 reagents sent with the pack, one is a formalin fixative and the other is a Calcium Chloride; form a nice clot. Now we just add 4 drops of the Blue reagent to the cell button and resuspend. Then add at least 5 drops of the Clear reagent to form a great clot. Then pull it out and wrap it in tissue paper.We have really increased the retrieval of cells Hope this helps >From: "Patterson, Patricia" >To: >Subject: [Histonet] Low Yield FNA samples >Date: Fri, 17 Nov 2006 17:13:49 -0600 > > >Greetings Histonetters! > >We have some very low yield FNA's here and we're trying to find a better >way to handle them to optimize what our Histology lab needs to deal >with. > > > >Currently we do a simple spin down with 95% and create a cell block. >But sometimes the amount we pull from tube is very small. > >We've tried a Fibrin clot method using plasma or thrombin - but our >method seems time consuming. > > > >Someone mentioned an agar cell method or a colloidal bag method but we >don't know anyone who is doing them. > >Any suggestions would be greatly appreciated. > > > >Thanks > >Pat Patterson > >Methodist Dallas Medical Center > >patpatterson@mhd.com > >P - 214-947-3538 > >F - 214-947-3524 > > > > > >*********************************************************************** > >This electronic transmission contains information from Methodist Health >System and should be considered confidential and privileged. The >information contained in the above messages is intended only for the >use of the individual(s) and entity(ies) named above. If you are not >the intended recipient, be aware that any disclosure, copying, >distribution, or use of this information is prohibited. If you receive >this transmission in error, please notify the sender immediately by >return e-mail. Methodist Health System, its subsidiaries and >affiliates hereby claim all applicable privileges related to the >transmission of this communication. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Ready for the world's first international mobile film festival celebrating the creative potential of today's youth? Check out Mobile Jam Fest for your a chance to WIN $10,000! www.mobilejamfest.com From anh2006 <@t> med.cornell.edu Sat Nov 18 10:37:11 2006 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Sat Nov 18 10:37:33 2006 Subject: [Histonet] Beta-Gal substrates Message-ID: Has anyone ever tried to stain for beta-galactosidase activity using magenta-gal and/or salmon-gal? If so, I am curious to know your experience and how they compare to X-Gal. Thanks, Andrea -- From AnthonyH <@t> chw.edu.au Sun Nov 19 23:15:40 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Nov 19 23:17:36 2006 Subject: [Histonet] Normal Controls for Cerb-B2 Message-ID: Hi all, Apart from Breast and Ovarian tumours (which are rare in pediatric pathology), does anyone know what normal tissue elements contain this protein that can be demonstrated using immunohistochemistry? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From Kemlo.Rogerson <@t> waht.swest.nhs.uk Mon Nov 20 02:32:50 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Nov 20 02:31:56 2006 Subject: [Histonet] 10 % Formalin Message-ID: <86ADE4EB583CE64799A9924684A0FBBFAFC9B9@wahtntex2.waht.swest.nhs.uk> Then buffer it to neutrality. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net A belief is purely an individual matter, and you cannot and must not organize it. --J. Krishnamurti This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From abright <@t> brightinstruments.com Mon Nov 20 04:28:32 2006 From: abright <@t> brightinstruments.com (Alan Bright) Date: Mon Nov 20 04:28:32 2006 Subject: [Histonet] freezing brain slices Message-ID: I go along with this method. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype User ID: dazzle0 -----Original Message----- From: Monfils, Paul [mailto:PMonfils@Lifespan.org] Sent: 17 November 2006 16:58 To: 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] freezing brain slices I have worked out a method for freezing thin tissues flat, that works well for me. I keep two stainless steel plates in the -80 freezer. These are actually stainless steel covers for staining dishes. I take a thin piece of polyethylene, about 3x3", cut from a plastic bag. Not a sandwich bag, a good quality heavy duty plastic bag like you might use for storing specimens. I put a bit of OCT on the plastic, place the specimen on it, add another drop of OCT on top, then place a second plastic square on top. With gentle pressure from a tongue depressor or similar flat object, I flatten and smooth the OCT and tissue between the two pieces of plastic. Then I take it to the freezer. I drop the plastic/OCT/tissue "sandwich" onto one of the pre-chilled steel plates, then quickly - I emphasise quickly! - place the other steel plate on top. The tissue is flattened, and freezes in a few seconds. If the tissue is soft, and I think the weight of the steel plate might crush or compress it too much, I place a microscope slide or similar spacer on each end of the bottom plate, place the tissue between them and quickly put the second plate on top. That way the OCT and tissue can't be compressed any more than the thickness of the spacer. I then remove the OCT/tissue wafer from between the layers of plastic, keeping it in the freezer. To mount it for sectioning without thawing, I lay the wafer on one of the steel plates, then take a frozen blank OCT block (frozen in the standard freezer, not the -80), soften the surface a bit with my finger, apply a bit of additional OCT to the face of the block, then before it can freeze, press it down onto the wafer on the cold plate. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bhewlett <@t> cogeco.ca Mon Nov 20 08:10:07 2006 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Mon Nov 20 08:10:13 2006 Subject: [Histonet] Normal Controls for Cerb-B2 References: Message-ID: <000601c70cad$95cacd40$6500a8c0@mainbox> Tony, The vast majority of normal epithelial cells express HER2 protein on their surface. However, you would not want to use these as a procedural control, since the objective is to demonstrate OVER-EXPRESSION of this normal protein. Detection of normal levels of HER2 would invalidate the test. Bryan ----- Original Message ----- From: "Tony Henwood" To: Sent: Monday, November 20, 2006 12:15 AM Subject: [Histonet] Normal Controls for Cerb-B2 Hi all, Apart from Breast and Ovarian tumours (which are rare in pediatric pathology), does anyone know what normal tissue elements contain this protein that can be demonstrated using immunohistochemistry? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From barrickstacey <@t> yahoo.com Mon Nov 20 08:16:23 2006 From: barrickstacey <@t> yahoo.com (Stacey Barrick) Date: Mon Nov 20 08:23:14 2006 Subject: [Histonet] freezing brain slices Message-ID: <20061120141623.2658.qmail@web54314.mail.yahoo.com> Thank you all for your advice. I will try some of the methods this week and see what works best for our tissue.. Thanks again! Have a good week! Stacey --------------------------------- Sponsored Link Mortgage rates near 39yr lows. $420,000 Mortgage for $1,399/mo - Calculate new house payment From dfvilleg <@t> mtu.edu Mon Nov 20 09:14:31 2006 From: dfvilleg <@t> mtu.edu (Diego Villegas) Date: Mon Nov 20 09:14:43 2006 Subject: [Histonet] Alizarin red S - Calcium Message-ID: <4561C657.1030005@mtu.edu> Hi all, The protocol for Alizarin red S to identify calcium suggest a solution pH 4.1 to 4.3. The Alizarin solution of our provider has a 4.5 to 5 pH. Could I use it, or is critical the pH? Diego Biomechanics Lab Michigan Technological University. From Dawn.Olszewski <@t> SGMC.ORG Mon Nov 20 09:42:37 2006 From: Dawn.Olszewski <@t> SGMC.ORG (Olszewski, Dawn) Date: Mon Nov 20 09:48:12 2006 Subject: [Histonet] labvision ihc stainer....problems Message-ID: <42A2A5ED3C4F2C4C8ECC956EB32040BD02A043AA@exchange.sgmc.org> Dear histonetters, I have been having IHC issues sporatically for awhile now and would appreciate any suggestions that might keep me from pulling out my hair. I am only giving out the name of the products we use to give you the complete picture. I am not trying to bash any particular company. I just need HELP!!!!! I have used Dako and Ventanna stainers in the past and know that every stainer has some issues. I am now using 2 Labvision stainers and was wondering if I am the only one having problems. I have experienced complete run failures as well as only 1-2 antibodies per run not working with no obvious reason for failure. Sometimes the control will be negative and the patient will work or neither will work or the control will be positive but the patient will be negative (that should have been pos.) so, you never really know if the results are acurate. But to complicate matters or frustrate me .... the whole process is sporatic with no rhyme or reason and it has happened on BOTH instruments. I can repeat the stains and all work fine or do 3 different slides with controls and patient side by side and 1 work and 2 fail . Crazy stuff like that. I do one step depar and AR in the pressure cooker and also use buffer with tween 20. I have had the service reps out here several times adjusting drop zones, etc. because the problem looks to be a flow issue. I am almost ready to give up.....Maybe I am overlooking something obvious I have just forgotten about . Thanks in advance for any suggestions. Dawn Olszewski HTL (ASCP) QIHC South Georgia Medical Center Valdosta, GA. From lorimroberts2 <@t> yahoo.com Mon Nov 20 12:43:19 2006 From: lorimroberts2 <@t> yahoo.com (Lori Roberts) Date: Mon Nov 20 12:43:28 2006 Subject: [Histonet] HM 335E user manual--thanks! Message-ID: <997160.24194.qm@web34311.mail.mud.yahoo.com> Hi there, Thanks to all who responded to my request for the Microm user manual. As many of you suggested, Richard-Allen is the place to contact for help with Microm instruments. A Richard-Allen tech consultant (thanks Curtis!) sent me a PDF within a few minutes of my Histonet posting. Lori --------------------------------- Sponsored Link Mortgage rates near 39yr lows. $510,000 Mortgage for $1,698/mo - Calculate new house payment From DBotsfor <@t> wrh.on.ca Mon Nov 20 13:22:30 2006 From: DBotsfor <@t> wrh.on.ca (Botsford, Daniel) Date: Mon Nov 20 13:27:10 2006 Subject: [Histonet] labvision ihc stainer....problems Message-ID: <331EE6D48DDFD51189E500508BBD360806D9855D@mail.wrh.on.ca> Hello Dawn Who manufactures your positive charged slide? We had ER cases where the positive control on the top of the slide stained and the a known positive patient case on the bottom half did not.The patient internal control did not stain. (One slide with the control on the top half and the patient on the bottom half) Traced the problem to slide manufactor. Very random. Manufacturer failed to have an even distribution of charge on the entire slide which interfered with staining. When we switched to Erie Manufactured Slides the problem disappeared. Sincerely Daniel Botsford Windsor Regional Hospital 1995 Lens Avenue Windsor, Ontario N8W 1L9 519-254-5577 ext 52373 519-254-6861 fax dbotsfor@wrh.on.ca Message: 7 Date: Mon, 20 Nov 2006 10:42:37 -0500 From: "Olszewski, Dawn" Subject: [Histonet] labvision ihc stainer....problems To: histonet@lists.utsouthwestern.edu Message-ID: <42A2A5ED3C4F2C4C8ECC956EB32040BD02A043AA@exchange.sgmc.org> Content-Type: text/plain; charset=utf-8 Dear histonetters, I have been having IHC issues sporatically for awhile now and would appreciate any suggestions that might keep me from pulling out my hair. I am only giving out the name of the products we use to give you the complete picture. I am not trying to bash any particular company. I just need HELP!!!!! I have used Dako and Ventanna stainers in the past and know that every stainer has some issues. I am now using 2 Labvision stainers and was wondering if I am the only one having problems. I have experienced complete run failures as well as only 1-2 antibodies per run not working with no obvious reason for failure. Sometimes the control will be negative and the patient will work or neither will work or the control will be positive but the patient will be negative (that should have been pos.) so, you never really know if the results are acurate. But to complicate matters or frustrate me .... the whole process is sporatic with no rhyme or reason and it has happened on BOTH instruments. I can repeat the stains and all work fine or do 3 different slides with controls and patient side by side and 1 work and 2 fail . Crazy stuff like that. I do one step depar and AR in the pressure cooker and also use buffer with tween 20. I have had the service reps out here several times adjusting drop zones, etc. because the problem looks to be a flow issue. I am almost ready to give up.....Maybe I am overlooking something obvious I have just forgotten about . Thanks in advance for any suggestions. Dawn Olszewski HTL (ASCP) QIHC South Georgia Medical Center Valdosta, GA. From johanna.jackson <@t> csc.mrc.ac.uk Mon Nov 20 13:23:48 2006 From: johanna.jackson <@t> csc.mrc.ac.uk (Jackson, Johanna) Date: Mon Nov 20 13:28:18 2006 Subject: [Histonet] RE: Alizarin red S - Calcium References: Message-ID: <2A1F3E27491CBF46BC14ACB3E5C7D89A01CEA596@icex3.ic.ac.uk> The pH or Alizarin Red is very critical so I would try and keep it as close to the recommended pH as possible. Also, be careful when you wash the section/cells with PBS or something as this really mucks it up... may be obvious but I found out the hard way!! Good luck! Jo Stem Cell Imaging MRC Clinical Sciences Centre Imperial College London Hammersmith Hospital Campus Du Cane Road London W12 0NN ------------------------------ Message: 6 Date: Mon, 20 Nov 2006 10:14:31 -0500 From: Diego Villegas Subject: [Histonet] Alizarin red S - Calcium To: histonet@lists.utsouthwestern.edu Message-ID: <4561C657.1030005@mtu.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hi all, The protocol for Alizarin red S to identify calcium suggest a solution pH 4.1 to 4.3. The Alizarin solution of our provider has a 4.5 to 5 pH. Could I use it, or is critical the pH? Diego Biomechanics Lab Michigan Technological University. From Jessica.Vacca <@t> HCAhealthcare.com Mon Nov 20 13:37:58 2006 From: Jessica.Vacca <@t> HCAhealthcare.com (Vacca Jessica) Date: Mon Nov 20 13:38:35 2006 Subject: [Histonet] Trade in that snow plow for a bucket and shovel to make your own sand castle Message-ID: <41E16A15CE78374EA45B57E0F94339B801C74BDA@ORLEV01.hca.corpad.net> Tired of the cold and looking for the sun..........Welcome to Florida! Brandon Regional Hospital (HCA facility) is looking for a histologist Full time, no weekends or Holidays. (those can be spent at the beach or local theme park) Brandon is a growing town, just between Orlando and Tampa. If you take the position, you have the great opportunity to work with a great staff- 2 lab aides, 3 Techs, 2 transcriptionists, and 3 great Doctors Job duties would consist of Routine histology, Immunohistochemistry, send outs Please contact me for information or send your resume'...... I look forward to hearing from you Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr. Brandon Fl 33511 (813) 571-5193 or (813) 681-5551 ext 2454 Jessica.Vacca@hcahealthcare.com From TJasper <@t> smdc.org Mon Nov 20 14:41:05 2006 From: TJasper <@t> smdc.org (Jasper, Thomas G.) Date: Mon Nov 20 14:41:26 2006 Subject: [Histonet] Licensing Message-ID: <1A9F2A6C5762524799A816F1F09744CF0143A493@SCREECH.ntcampus.smdc.org> Would anyone be willing to share their opinion about licensing to do Histology? Up here in Minnesota they are kicking around something called the "Medical Laboratory Science Practice Act". Still in the very preliminary stages but the goal is to have it enacted by the legislature. My understanding in a nutshell - Histotechnologists and Histotechnicians would be issued licenses based on degree held (Bachelors vs. Associates) and successful certification by the ASCP-BOR. There is no stipulation about HT/HTL in the draft I've seen, I'm assuming one or the other would suffice. License renewal would then occur every 3 years. The conditions for renewal are 12 hours of CE annually and a fee (of course) yet to be determined (and I'm sure subject to change/increase). This act would apply to all Laboratorians, I've just mentioned the Histology stuff. I must admit I am somewhat leery about this whole thing mainly because the world these days is already buried in bureaucracy. I am however open to views on this topic. If I understand correctly Florida already has such legislation in place (for years). And New York was thinking about it or may have done it? Anyway, opinions appreciated. Thanks, Thomas Jasper HT (ASCP) BAS Anatomic Pathology Supervisor SMDC Clinical Laboratory Duluth, MN tjasper@smdc.org This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From rjbuesa <@t> yahoo.com Mon Nov 20 15:09:50 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Nov 20 15:10:02 2006 Subject: [Histonet] Licensing In-Reply-To: <1A9F2A6C5762524799A816F1F09744CF0143A493@SCREECH.ntcampus.smdc.org> Message-ID: <20061120210950.17426.qmail@web61221.mail.yahoo.com> Thomas: Getting a licensure backed by studies is the single most effective way of receiving the respect we all deserve. It is not just being able to work in a consistent way, it is the background required to do an effective work. In the UK all lab workers have to study at the bachelors level to be certified as a BMS = Bio Medical Scientist, divided into 3 categories according with experience. It would be just wonderful if all working in histology would be able to understand many of the things routinely done every day. The sad thing is that this is not the case. It is not the same to play "by ear" than being able to read or write music. This does not make you a better player, but it surely helps. That is my honest opinion! Ren? J. "Jasper, Thomas G." wrote: Would anyone be willing to share their opinion about licensing to do Histology? Up here in Minnesota they are kicking around something called the "Medical Laboratory Science Practice Act". Still in the very preliminary stages but the goal is to have it enacted by the legislature. My understanding in a nutshell - Histotechnologists and Histotechnicians would be issued licenses based on degree held (Bachelors vs. Associates) and successful certification by the ASCP-BOR. There is no stipulation about HT/HTL in the draft I've seen, I'm assuming one or the other would suffice. License renewal would then occur every 3 years. The conditions for renewal are 12 hours of CE annually and a fee (of course) yet to be determined (and I'm sure subject to change/increase). This act would apply to all Laboratorians, I've just mentioned the Histology stuff. I must admit I am somewhat leery about this whole thing mainly because the world these days is already buried in bureaucracy. I am however open to views on this topic. If I understand correctly Florida already has such legislation in place (for years). And New York was thinking about it or may have done it? Anyway, opinions appreciated. Thanks, Thomas Jasper HT (ASCP) BAS Anatomic Pathology Supervisor SMDC Clinical Laboratory Duluth, MN tjasper@smdc.org This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Check out the all-new Yahoo! Mail beta - Fire up a more powerful email and get things done faster. From godsgalnow <@t> aol.com Mon Nov 20 15:22:25 2006 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Mon Nov 20 15:22:40 2006 Subject: [Histonet] Licensing In-Reply-To: <1A9F2A6C5762524799A816F1F09744CF0143A493@SCREECH.ntcampus.smdc.org> References: <1A9F2A6C5762524799A816F1F09744CF0143A493@SCREECH.ntcampus.smdc.org> Message-ID: <8C8DB011BFE0AA1-F70-190A@FWM-D30.sysops.aol.com> We've been doing it that way for years in Florida. Every professional position in the lab from technician to supervisor to cytotech, all have different levels of education and certification. We renew every 2 years and must maintain continuing education. Roxanne Soto HT(ASCP)QIHC Lab Manager Physicians RightPath Tampa, Florida -----Original Message----- From: TJasper@smdc.org To: histonet@lists.utsouthwestern.edu Sent: Mon, 20 Nov 2006 3:41 PM Subject: [Histonet] Licensing Would anyone be willing to share their opinion about licensing to do Histology? Up here in Minnesota they are kicking around something called the "Medical Laboratory Science Practice Act". Still in the very preliminary stages but the goal is to have it enacted by the legislature. My understanding in a nutshell - Histotechnologists and Histotechnicians would be issued licenses based on degree held (Bachelors vs. Associates) and successful certification by the ASCP-BOR. There is no stipulation about HT/HTL in the draft I've seen, I'm assuming one or the other would suffice. License renewal would then occur every 3 years. The conditions for renewal are 12 hours of CE annually and a fee (of course) yet to be determined (and I'm sure subject to change/increase). This act would apply to all Laboratorians, I've just mentioned the Histology stuff. I must admit I am somewhat leery about this whole thing mainly because the world these days is already buried in bureaucracy. I am however open to views on this topic. If I understand correctly Florida already has such legislation in place (for years). And New York was thinking about it or may have done it? Anyway, opinions appreciated. Thanks, Thomas Jasper HT (ASCP) BAS Anatomic Pathology Supervisor SMDC Clinical Laboratory Duluth, MN tjasper@smdc.org This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From Charles.Nicholson <@t> carolinashealthcare.org Mon Nov 20 15:12:22 2006 From: Charles.Nicholson <@t> carolinashealthcare.org (Nicholson, Charles) Date: Mon Nov 20 15:30:24 2006 Subject: [Histonet] Klatskin trichrome Message-ID: <27683EDE37CAAB4A8A4C047C1040E9280333F4EA@dcr-xchg-05.Carolinas.org> Hi, I'm looking for a prodcedure for a Klatskin trichrome stain. Does anyone have one that they could share? Charles Nicholson, M.D. Dept. of Pathology Cleveland Regional Medical Center Shelby, NC 28150 704-487-3155 ----------------------------------------- This electronic message may contain information that is confidential and/or legally privileged. It is intended only for the use of the individual(s) and entity named as recipients in the message. If you are not an intended recipient of this message, please notify the sender immediately and delete the material from any computer. Do not deliver, distribute or copy this message, and do not disclose its contents or take any action in reliance on the information it contains. Thank you. From pathrm35 <@t> charter.net Mon Nov 20 16:06:01 2006 From: pathrm35 <@t> charter.net (pathrm35@charter.net) Date: Mon Nov 20 16:06:10 2006 Subject: [Histonet] Licensing Message-ID: <107502245.1164060361972.JavaMail.root@fepweb12> I have mixed feelings about state licensing. In Florida the only thing it does is limit how many techs there are so the techs can dictate their salaries. This is good for them. This is frustrating if you are a supervisor trying to hire a quality tech. You basically have a small work force to choose from and you have a good chance of paying good money for a poor tech. I personally think we should have mandatory set national standards (education and experience) across the board that each state needs to recognize. ASCP is great but it is not mandatory, esp. in research or veterinary histology. The only way state licensing will turn histology into a recognized profession is if each state requires very high educational and experience levels prior to licensing. As Joe Nocito use to say -- let the flaming begin. Ron Martin, BS, HT (ASCP) HTL, QIHC Florida licensed Histology Supervisor ---- "Jasper wrote: > Would anyone be willing to share their opinion about licensing to do Histology? Up here in Minnesota they are kicking around something called the "Medical Laboratory Science Practice Act". Still in the very preliminary stages but the goal is to have it enacted by the legislature. > My understanding in a nutshell - Histotechnologists and Histotechnicians would be issued licenses based on degree held (Bachelors vs. Associates) and successful certification by the ASCP-BOR. There is no stipulation about HT/HTL in the draft I've seen, I'm assuming one or the other would suffice. License renewal would then occur every 3 years. The conditions for renewal are 12 hours of CE annually and a fee (of course) yet to be determined (and I'm sure subject to change/increase). > This act would apply to all Laboratorians, I've just mentioned the Histology stuff. I must admit I am somewhat leery about this whole thing mainly because the world these days is already buried in bureaucracy. I am however open to views on this topic. If I understand correctly Florida already has such legislation in place (for years). And New York was thinking about it or may have done it? Anyway, opinions appreciated. > Thanks, > > Thomas Jasper HT (ASCP) BAS > Anatomic Pathology Supervisor > SMDC Clinical Laboratory > Duluth, MN > tjasper@smdc.org > > > > This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Mon Nov 20 16:14:03 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Nov 20 16:15:46 2006 Subject: [Histonet] Normal Controls for Cerb-B2 Message-ID: Bryan, You are absolutely right. This raises the question of thresholds. At what dilution of antibody should staining reflect over-expression? How many labs would call a borderline over-expression positive? This would depend on the dilution of the Cerb-B2 antibody as well as the sensitivity of the detection system. I think I will need to peruse the external QAP figures to see if this is an issue. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: Bryan Hewlett [mailto:bhewlett@cogeco.ca] Sent: Tuesday, 21 November 2006 1:10 AM To: Tony Henwood; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Normal Controls for Cerb-B2 Tony, The vast majority of normal epithelial cells express HER2 protein on their surface. However, you would not want to use these as a procedural control, since the objective is to demonstrate OVER-EXPRESSION of this normal protein. Detection of normal levels of HER2 would invalidate the test. Bryan ----- Original Message ----- From: "Tony Henwood" To: Sent: Monday, November 20, 2006 12:15 AM Subject: [Histonet] Normal Controls for Cerb-B2 Hi all, Apart from Breast and Ovarian tumours (which are rare in pediatric pathology), does anyone know what normal tissue elements contain this protein that can be demonstrated using immunohistochemistry? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From katherine-walters <@t> uiowa.edu Mon Nov 20 16:50:29 2006 From: katherine-walters <@t> uiowa.edu (Walters, Katherine S) Date: Mon Nov 20 16:50:43 2006 Subject: [Histonet] NSH CD Message-ID: I just got my CD from the NSH meeting in Phoenix. The cover letter says that it contains (among other useful information) seminar handouts. I was glad, as one of my classes didn't have 1/2 of their handouts ready, and I have not gotten it. However, I can't find the handouts on the CD, only the workshop abstracts. Did anyone else have this problem? Kathy From gcallis <@t> montana.edu Mon Nov 20 18:13:57 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Nov 20 18:14:12 2006 Subject: [Histonet] NSH CD ATTN: Workshop #40 attendees In-Reply-To: References: Message-ID: <6.0.0.22.1.20061120162553.01b3c7f8@gemini.msu.montana.edu> The CD is what you would have gotten in the older style 3 ring binder. Handouts to half day or full day workshops have never been included in that NSH Symposium/Convention binder. Scientific seminar handouts have always been in the binder. That is what I understood was going on with this new CD. So if your class was a WORKSHOP instead of a scientific session, then the handout is not part of that CD. Oh dear, I repeating myself - late in the day. Workshop handouts remain exclusive for those paying for the workshops even though the workshop abstracts are always accessible, as found on the NSH website and in the convention brochure, binder or CD for selection purposes. If you have not received a workshop handout or a proper one that is readable, contact the NSH office to get the handout you have paid for. If you are desparate, you could also ask the workshop director for a copy. Unfortunately, this also happened in my portion of workshop #40 when there was failure to have a printed handout for whatever reason, lost? Shipping error? What attendees ended up receiving was a 6 slide per page powerpoint handout that no one could read because of a last minute copying effort 3 hours before the workshop. It was a tough day, but NSH graciously promised attendees would receive the REAL handout, although after the fact. A word to the wise for those giving workshops in the future - provide a CD of your handout!!!! This is allowed and if the presentation is jam packed with information, a nice way for attendees to see better color photos and study at their leisure. If anyone is looking in and never received part 2 of Workshop #40, let me know, I will send the CD to them. It will be better than a black and white handout anyway. At 03:50 PM 11/20/2006, you wrote: >I just got my CD from the NSH meeting in Phoenix. The cover letter says >that it contains (among other useful information) seminar handouts. I >was glad, as one of my classes didn't have 1/2 of their handouts ready, >and I have not gotten it. However, I can't find the handouts on the CD, >only the workshop abstracts. Did anyone else have this problem? > >Kathy > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From bhewlett <@t> cogeco.ca Mon Nov 20 19:44:39 2006 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Mon Nov 20 19:45:10 2006 Subject: [Histonet] Normal Controls for Cerb-B2 References: Message-ID: <001301c70d0e$a547fc50$6500a8c0@mainbox> Tony, It does indeed raise the question of detection threshold!! If one is performing a titre to judge working antibody dilution for breast tumour HER2 overexpression, the procedural control slides should contain several different samples of; 1) normal breast epithelium, 2) breast tumours known to have normal levels of expression (i.e. non-amplified - confirmed by FISH), 3) breast tumours known to have levels of overexpression (5-20x amplified - confirmed by FISH). The working dilution should be adjusted to the HIGHEST dilution at which; samples 1&2 are negative and sample 3 shows complete circumferential staining of at least 50% of tumour cells. All of these samples should have been optimally fixed in NBF (minimum of 24 hours at RT). An alternative is to use optimally fixed blocks of cell lines with known levels of HER2 expression (these are available from couple of suppliers). Once you have determined a working dilution, confirm with validation studies according to CLSI guidelines. Locally, all 2+ positives are considered equivocal and reflexed to FISH for confirmation. Check the UKNEQAS web site for insight about EQA for HER2. Regards, Bryan ----- Original Message ----- From: "Tony Henwood" To: "Bryan Hewlett" ; Sent: Monday, November 20, 2006 5:14 PM Subject: RE: [Histonet] Normal Controls for Cerb-B2 Bryan, You are absolutely right. This raises the question of thresholds. At what dilution of antibody should staining reflect over-expression? How many labs would call a borderline over-expression positive? This would depend on the dilution of the Cerb-B2 antibody as well as the sensitivity of the detection system. I think I will need to peruse the external QAP figures to see if this is an issue. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: Bryan Hewlett [mailto:bhewlett@cogeco.ca] Sent: Tuesday, 21 November 2006 1:10 AM To: Tony Henwood; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Normal Controls for Cerb-B2 Tony, The vast majority of normal epithelial cells express HER2 protein on their surface. However, you would not want to use these as a procedural control, since the objective is to demonstrate OVER-EXPRESSION of this normal protein. Detection of normal levels of HER2 would invalidate the test. Bryan ----- Original Message ----- From: "Tony Henwood" To: Sent: Monday, November 20, 2006 12:15 AM Subject: [Histonet] Normal Controls for Cerb-B2 Hi all, Apart from Breast and Ovarian tumours (which are rare in pediatric pathology), does anyone know what normal tissue elements contain this protein that can be demonstrated using immunohistochemistry? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Tue Nov 21 04:53:44 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCZVED)) Date: Tue Nov 21 04:59:19 2006 Subject: [Histonet] NSH CD Message-ID: I don't think they meant workshop handouts....you can only get those if you paid to attend that particular workshop. However, the seminars are included on the CD. For example, under Monday you will see a list of the Clinical and VIR Seminars. Click on the one you are interested in and there is an overview. Click again on Materials for the actual hand-out. Just go through the program for all of the seminars (Monday through Wednesday) and you should find everything there. Also, you can print out the info. regarding the Sunday morning Culling Memorial and International Lectures as well. Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Walters, Katherine S Sent: Monday, November 20, 2006 5:50 PM To: histonet@pathology.swmed.edu Subject: [Histonet] NSH CD I just got my CD from the NSH meeting in Phoenix. The cover letter says that it contains (among other useful information) seminar handouts. I was glad, as one of my classes didn't have 1/2 of their handouts ready, and I have not gotten it. However, I can't find the handouts on the CD, only the workshop abstracts. Did anyone else have this problem? Kathy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jmahoney <@t> alegent.org Tue Nov 21 07:47:42 2006 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Tue Nov 21 07:48:11 2006 Subject: [Histonet] NSH CD Message-ID: Kathy, the CD includes seminar handouts, not workshop handouts. The seminars are the "free" classes that are shorter than the half day or full day workshops. Jan Omaha >>> "Walters, Katherine S" 11/20/2006 4:50 PM >>> I just got my CD from the NSH meeting in Phoenix. The cover letter says that it contains (among other useful information) seminar handouts. I was glad, as one of my classes didn't have 1/2 of their handouts ready, and I have not gotten it. However, I can't find the handouts on the CD, only the workshop abstracts. Did anyone else have this problem? Kathy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From katherine-walters <@t> uiowa.edu Tue Nov 21 07:51:06 2006 From: katherine-walters <@t> uiowa.edu (Walters, Katherine S) Date: Tue Nov 21 07:51:18 2006 Subject: [Histonet] NSH CD ATTN: Workshop #40 attendees Message-ID: Thanks Gayle, you are always so helpful, but the one I needed was workshop #39. Did anyone get these? It was only Keith's half that didn't come out, I emailed him a couple of months ago, I may have to do it again.. Kathy -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: Monday, November 20, 2006 6:14 PM To: Walters, Katherine S; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] NSH CD ATTN: Workshop #40 attendees The CD is what you would have gotten in the older style 3 ring binder. Handouts to half day or full day workshops have never been included in that NSH Symposium/Convention binder. Scientific seminar handouts have always been in the binder. That is what I understood was going on with this new CD. So if your class was a WORKSHOP instead of a scientific session, then the handout is not part of that CD. Oh dear, I repeating myself - late in the day. Workshop handouts remain exclusive for those paying for the workshops even though the workshop abstracts are always accessible, as found on the NSH website and in the convention brochure, binder or CD for selection purposes. If you have not received a workshop handout or a proper one that is readable, contact the NSH office to get the handout you have paid for. If you are desparate, you could also ask the workshop director for a copy. Unfortunately, this also happened in my portion of workshop #40 when there was failure to have a printed handout for whatever reason, lost? Shipping error? What attendees ended up receiving was a 6 slide per page powerpoint handout that no one could read because of a last minute copying effort 3 hours before the workshop. It was a tough day, but NSH graciously promised attendees would receive the REAL handout, although after the fact. A word to the wise for those giving workshops in the future - provide a CD of your handout!!!! This is allowed and if the presentation is jam packed with information, a nice way for attendees to see better color photos and study at their leisure. If anyone is looking in and never received part 2 of Workshop #40, let me know, I will send the CD to them. It will be better than a black and white handout anyway. At 03:50 PM 11/20/2006, you wrote: >I just got my CD from the NSH meeting in Phoenix. The cover letter says >that it contains (among other useful information) seminar handouts. I >was glad, as one of my classes didn't have 1/2 of their handouts ready, >and I have not gotten it. However, I can't find the handouts on the CD, >only the workshop abstracts. Did anyone else have this problem? > >Kathy > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From aubrey <@t> nsh.org Tue Nov 21 08:25:38 2006 From: aubrey <@t> nsh.org (Aubrey Wanner) Date: Tue Nov 21 08:25:46 2006 Subject: [Histonet] NSH Convention CD Message-ID: I recently read a post that asked "where to find the seminar handouts on the NSH Convention CD" . To find the seminar handouts click on the day of the seminar on the main menu page (Monday, Tuesday or Wednesday). Then click on the link at the top of the page that says "Clinical and VIR Seminars" from there it will take you to a list of the seminars for that day. Click on the seminar you want and their will be a "materials" link for the handouts. If you have any other questions please feel free to contact the NSH Office, 301-262-6221. Mrs. Aubrey M.J. Wanner Meeting Manager, Annual Symposium/Convention Managing Editor, Journal of Histotechnology National Society for Histotechnology 4201 Northview Drive | Suite 502 Bowie, MD 20716 P | 301.262.6221 x.18 F | 301.262.9188 http://www.nsh.org/ From c.weaver <@t> vla.defra.gsi.gov.uk Tue Nov 21 08:26:58 2006 From: c.weaver <@t> vla.defra.gsi.gov.uk (Weaver, Colin) Date: Tue Nov 21 08:27:30 2006 Subject: [Histonet] Staining technique for Lawsonia Message-ID: <7A885E8FE1C71C488D974EC601FAA6900D0A80@vla-exchn1.cvlnt.vla.gov.uk> Hi everyone - I wonder if anyone can help. One of my colleagues has asked about using Costers(not sure about spelling) staining technique for the demonastration of Lawsonia in pig intestines. I have never heard of it , can find no hits in Histonet archive and nothing on google. Any help would be most useful. Colin Weaver Veterinary Laboratories Agency (VLA) This email and any attachments is intended for the named recipient only. If you have received it in error you have no authority to use, disclose, store or copy any of its contents and you should destroy it and inform the sender. Whilst this email and associated attachments will have been checked for known viruses whilst within VLA systems we can accept no responsibility once it has left our systems. Communications on VLA's computer systems may be monitored and/or recorded to secure the effective operation of the system and for other lawful purposes. From petepath <@t> yahoo.com Tue Nov 21 08:31:52 2006 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Tue Nov 21 08:31:59 2006 Subject: [Histonet] Pathologist Assistant opening Message-ID: <34723.20529.qm@web30413.mail.mud.yahoo.com> We have an opening for a pathologist's assistant at Hackensack University Medical Center located in Bergan county New Jersey. Competative salary and benifits. Great experience in a very busy academic setting. Interested candidates can contact Jackie Brown our histology lab manager at: jbrown@humed.com Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com From bhewlett <@t> cogeco.ca Tue Nov 21 09:53:13 2006 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Tue Nov 21 09:53:28 2006 Subject: Fw: [Histonet] Staining technique for Lawsonia Message-ID: <000301c70d85$27643ac0$6500a8c0@mainbox> ----- Original Message ----- From: "Bryan Hewlett" To: "Weaver, Colin" Sent: Tuesday, November 21, 2006 10:01 AM Subject: Re: [Histonet] Staining technique for Lawsonia > Hi Colin, > > Koster's stain is a modification of Kinyoun's stain for acid-fast > organisms. > I would think that any of the many available modifications for > acid/alcohol fast organisms would work. > > Bryan > > > ----- Original Message ----- > From: "Weaver, Colin" > To: > Sent: Tuesday, November 21, 2006 9:26 AM > Subject: [Histonet] Staining technique for Lawsonia > > > Hi everyone - I wonder if anyone can help. One of my colleagues has > asked about using Costers(not sure about spelling) staining technique > for the demonastration of Lawsonia in pig intestines. I have never heard > of it , can find no hits in Histonet archive and nothing on google. Any > help would be most useful. > > Colin Weaver > > > Veterinary Laboratories Agency (VLA) > > This email and any attachments is intended for the named recipient > only. > If you have received it in error you have no authority to use, disclose, > store or copy any of its contents and you should destroy it and inform > the sender. > Whilst this email and associated attachments will have been checked > for known viruses whilst within VLA systems we can accept no > responsibility once it has left our systems. > Communications on VLA's computer systems may be monitored and/or > recorded to secure the effective operation of the system and for other > lawful purposes. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Rcartun <@t> harthosp.org Tue Nov 21 10:15:43 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Nov 21 10:15:59 2006 Subject: [Histonet] Coxiella burnettii Message-ID: <4562DFDF0200007700002EF1@hcnwgwds01.hh.chs> Anyone doing IHC or PCR for Coxiella burnettii on formalin-fixed, paraffin-embedded human tissue for diagnosis? Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From GDawson <@t> dynacaremilwaukee.com Tue Nov 21 10:25:54 2006 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Tue Nov 21 10:26:05 2006 Subject: [Histonet] NSH CD Message-ID: All, I attended WS#018 "Flourescence In-Situ Hybridization in Anatomic Pathology" & no handouts were available. The presenter got all of our addresses & e-mail addresses & stated that he would either email us the workshop handout or burn it to CD & mail it to us. Did anyone receive this one? I am still waiting. Thanx, Glen Dawson Milwaukee, WI From thoward <@t> unm.edu Tue Nov 21 10:41:10 2006 From: thoward <@t> unm.edu (Tamara A Howard) Date: Tue Nov 21 10:41:20 2006 Subject: [Histonet] oops - samples directly to paraffin Message-ID: Help! I need the collective wisdom of the HistoNet this morning. Some students (who should know better) loaded their samples on to the processor yesterday afternoon but didn't have it set up correctly; the basket went directly in to one of the paraffin beakers and they didn't notice the error. Their PI went a few hours later to check up on the run and caught it; she put the cassettes directly in a beaker of buffer. Now we are wondering about the best way to rescue the samples: Take them to xylene-substitute to deparaffinize *OR* warm them up to melt the paraffin and fish out the pieces? Or some other brilliant solution? The tissue pieces are too small to chip out of the solid paraffin without risking a lot of damage. These were mouse bits destined for IHC - are they totally screwed? Advice, experience, horror stories all welcome. Thanks! Tamara *************************** Tamara Howard Cell Biology & Physiology UNM-HSC Albuquerque, NM *************************** From PMonfils <@t> Lifespan.org Tue Nov 21 12:27:36 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Tue Nov 21 12:28:00 2006 Subject: [Histonet] Re: oops - samples directly to paraffin Message-ID: <4EBFF65383B74D49995298C4976D1D5E273BB4@LSRIEXCH1.lsmaster.lifespan.org> Well, that's pretty bad. Not as bad as the time the heating unit on our processing chamber malfunctioned, and melted all the cassettes into one bubbling stew of liquified plastic and tissue bits. But still, bad enough. HOPEFULLY the tissue samples were well fixed before they were put on the processor? If they were unfixed tissues that were intended to go through fixative on the processor, but went directly into paraffin instead, then I don't have much hope for them, either in terms of IHC or morphology. But well fixed tissue can withstand a lot of abuse and still come out decent. I would not melt the paraffin if possible. There is too much danger of dessicating the small tissue samples with heat (some of this may already have occurred while they were in liquid paraffin). I would try to remove as much paraffin as possible manually. The paraffin has not infiltrated into the tissue, since the tissue samples were in aqueous medium immediately before going into the paraffin. You may find that the cassettes come loose from the paraffin fairly easily once you break some of the paraffin away. Actually, refrigerating the beaker for a while may facilitate this process. Also, if you open a cassette you will probably find that you don't have to "chip" the tissue out of the paraffin. There should be no bonding of the wax to the tissue, and any piece of tissue that is exposed can probably be lifted out of the paraffin easily with forceps, and placed into a new cassette. If it appears that you have gotten rid of almost all the paraffin manually, then I would just put the samples, in new cassettes, back on the processor, and process as usual. If you are not able to pick off most of the paraffin, then it will be necessary to dissolve the remaining paraffin with your xylene substitute. After that, I would put them on the processor directly from the xylene substitute, and process as usual. The alcohols will remove both the water and the residual xylene substitute from the tissues, and hopefully they will then infiltrate properly. Good luck! Let us know how you make out. From ROrr <@t> enh.org Tue Nov 21 13:05:22 2006 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Tue Nov 21 13:05:36 2006 Subject: [Histonet] RE: Histonet Digest, Vol 36, Issue 26 Message-ID: HI Dawn, Let's eliminate some variables here. Here are some suggestions....one variable at a time. The staining racks themselves can be warped. Lay them on the counter and scrutinize them for any areas that don't look level. Load the racks with slides and place the racks in the stainer, as if starting a run. Place the leveling device on each and every slide to see if you have a problem with the racks. Some of the Lab Vision models allow for a fine tuning adjustment in the level of the racks while they are in the holders in the stainer. There may be little screws on either end of the rack holder on your stainer...but you might need a field service repair person to help out on this. Sometimes you can run into issues with the leveling once the sink has been removed (the bottom part) and then put back into place. Also, Are you switching your slide racks between the stainers? I am not able to supply any validated evidence to the following, but in my experience only, I have found that some paraffins don't give the best result when using the one step method in a pressure cooker...go back to the xylene to depar for awhile...see if that might be causing some of the patchy staining. You might be fighting a few problems all at the same time. When you start your run, are you loading the slides out of a buffer solution or water? Use a buffer solution...with the tween....I use Biocare's Automatic wash buffer solution...it works great...Are you adding tween 20 to your rinse buffer? Or does is come already in the rinse buffer? I use two drop zones, 100 microliters each...one at the top of the slide and the other at the bottom...it gets the center covered very nicely...I am speaking for my lab... I know you might not have all day to do this, but if it was me having these problems, I would stand there and watch each and every step occur on each and every slide...run as full a run as you dare so you can detect any trends...since some slides work and others don't... I'll bet you'll be able to see if you are having any problems with the slides or the buffers giving you any leveling problems or any reagent spread problems if you can observe the entire process. I have some experience with the stainer being level and the racks being level, but the racks IN the stainer are not... Please feel free to give me a call, in case you need any clarification from my scream of consciousness. Hope this helps. Becky Becky Orr CLA,HT(ASCP)QIHC Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 Message: 7 Date: Mon, 20 Nov 2006 10:42:37 -0500 From: "Olszewski, Dawn" Subject: [Histonet] labvision ihc stainer....problems To: histonet@lists.utsouthwestern.edu Message-ID: <42A2A5ED3C4F2C4C8ECC956EB32040BD02A043AA@exchange.sgmc.org> Content-Type: text/plain; charset=utf-8 Dear histonetters, I have been having IHC issues sporatically for awhile now and would appreciate any suggestions that might keep me from pulling out my hair. I am only giving out the name of the products we use to give you the complete picture. I am not trying to bash any particular company. I just need HELP!!!!! I have used Dako and Ventanna stainers in the past and know that every stainer has some issues. I am now using 2 Labvision stainers and was wondering if I am the only one having problems. I have experienced complete run failures as well as only 1-2 antibodies per run not working with no obvious reason for failure. Sometimes the control will be negative and the patient will work or neither will work or the control will be positive but the patient will be negative (that should have been pos.) so, you never really know if the results are acurate. But to complicate matters or frustrate me .... the whole process is sporatic with no rhyme or reason and it has happened on BOTH instruments. I can repeat the stains and all work fine or do 3 different slides with controls and patient side by side and 1 work and 2 fail . Crazy stuff like that. I do one step depar and AR in the pressure cooker and also use buffer with tween 20. I have had the service reps out here several times adjusting drop zones, etc. because the problem looks to be a flow issue. I am almost ready to give up.....Maybe I am overlooking something obvious I have just forgotten about . Thanks in advance for any suggestions. Dawn Olszewski HTL (ASCP) QIHC South Georgia Medical Center Valdosta, GA. From akbitting <@t> geisinger.edu Tue Nov 21 13:33:59 2006 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Tue Nov 21 13:34:16 2006 Subject: [Histonet] Colored marking beads Message-ID: <45630E57020000C9000021B9@GHSGWIANW5V.GEISINGER.EDU> Does anyone know what vendors sell small colored beads to put into your tissue cassettes before processing? Our P.A. saw them in a flyer somewhere but he can't remember where. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From Anna.Yates <@t> se.amedd.army.mil Tue Nov 21 13:46:44 2006 From: Anna.Yates <@t> se.amedd.army.mil (Yates, Anna M Ms BACH) Date: Tue Nov 21 13:48:02 2006 Subject: [Histonet] SUBSCRIBE Message-ID: <17B8554B178BCC42BABB83121934086C01197919@amedmlsermc133> FOR A COLLEGUE: timothy.neal@se.amedd.army.mil From Kristopher.Kalleberg <@t> unilever.com Tue Nov 21 13:50:38 2006 From: Kristopher.Kalleberg <@t> unilever.com (Kalleberg, Kristopher) Date: Tue Nov 21 13:50:48 2006 Subject: [Histonet] temp position opening Message-ID: <0E6BC087F70F9C47ACFF2C203D6E329C018F9D5C@NTRSEVS30002.s3.ms.unilever.com> We are looking to fill a temporary position in histology in our lab in Trumbull, CT. We are looking for someone who can independently carry out routine sample preparation, sectioning and microscopy of skin tissue samples. Primarily interested in routine paraffin embedding and occasional sectioning of frozen skin tissue samples may be needed. Experience with immuno histochemistry would be desirable so would be experience in ultramicrotomy for transmission electron microscopy. The position is initially for 6 months and possibly renewable for another six months. Associates degree and/or Bachelor's degree (Biology/Science related degree) required. Please contact me if interested. Thanks, Manoj Manoj Misra, PhD Head Microstructure Imaging Unilever R&D 40 Merritt Boulevard Trumbull, CT 06611 (203) 381-5743 (Phone) (203) 381-5497 (Fax) From Anna.Yates <@t> se.amedd.army.mil Tue Nov 21 13:46:44 2006 From: Anna.Yates <@t> se.amedd.army.mil (Yates, Anna M Ms BACH) Date: Tue Nov 21 13:58:29 2006 Subject: [Histonet] SUBSCRIBE Message-ID: <17B8554B178BCC42BABB83121934086C01197925@amedmlsermc133> FOR A COLLEGUE: timothy.neal@se.amedd.army.mil From ancillarypath <@t> mac.com Tue Nov 21 14:14:18 2006 From: ancillarypath <@t> mac.com (ancillarypath@mac.com) Date: Tue Nov 21 14:14:25 2006 Subject: [Histonet] Re: FISH course CD Message-ID: <3996EDB4-DB02-4609-8A06-6B190A4171A2@mac.com> Dear Colleagues, This is Hadi Yaziji. I am the 'presenter' who gave the FISH course at the NSH meeting and I apologize for all of you who waited patiently to receive the CD. I think there was a major misunderstanding on my part: I thought that people who are interested in the file will email me to give me instructions how to send the CD. Those of you who are interested in the CD (like Glen), can you please email me back (privately) so I can actually send you login and password information to download the pdf file from our ftp server? This way you will save my office people a lot of time burning and mailing the CDs. Those of you who still want the CD will get it, but please indicate your preference and include a physical address in your reply email. Thank you for understanding and my sincere apologies to those who've been patiently waiting. Best regards, Hadi Yaziji, M.D. President, Ancillary Pathways, LLC. 7000 SW 62nd Ave, Suite PH-C Miami, FL 33143 Phone: 305.740.4440 Cell: 786.266.5725 Fax: 786-513-0175 www.ancillarypath.com From SHARON.OSBORN <@t> SPCORP.COM Tue Nov 21 14:52:28 2006 From: SHARON.OSBORN <@t> SPCORP.COM (Osborn, Sharon) Date: Tue Nov 21 14:52:38 2006 Subject: [Histonet] rescue tissues Message-ID: <9A919A5D70313A4D9C56A02571087408C54543@kenmsg40.us.schp.com> Tamara, 1. Wash the tissues as is in cassettes with the paraffin under cool running water for 5-10 min to remove the buffer. Using paper toweling, pat off the excess water. 2. Allow to air dry or, place into oven to evaporate the water and to melt off excess paraffin. 3. Place cassettes with melted outside paraffin into xylene or xylene substitute for 30-45 min X3 changes. 4. Place into 100% alcohol x2 changes for 30 min each. Continue until you have the 70% alcohol then you can properly process on the correct program for the machine. Essentially, you are running the tissues backwards down to the 70% alcohol then processing properly. I assume the tissues were already fixed a correct amount of time. If not, then you need to start in the 10% NBF on the processor. Sharon Osborn SPBioPharma Palo Alto, CA Date: Tue, 21 Nov 2006 09:41:10 -0700 From: "Tamara A Howard" Subject: [Histonet] oops - samples directly to paraffin To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="ISO-8859-1"; format="flowed" Help! I need the collective wisdom of the HistoNet this morning. Some students (who should know better) loaded their samples on to the processor yesterday afternoon but didn't have it set up correctly; the basket went directly in to one of the paraffin beakers and they didn't notice the error. Their PI went a few hours later to check up on the run and caught it; she put the cassettes directly in a beaker of buffer. Now we are wondering about the best way to rescue the samples: Take them to xylene-substitute to deparaffinize *OR* warm them up to melt the paraffin and fish out the pieces? Or some other brilliant solution? The tissue pieces are too small to chip out of the solid paraffin without risking a lot of damage. These were mouse bits destined for IHC - are they totally screwed? Advice, experience, horror stories all welcome. Thanks! Tamara *************************** Tamara Howard Cell Biology & Physiology UNM-HSC Albuquerque, NM ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From tbraud <@t> holyredeemer.com Tue Nov 21 14:55:00 2006 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Tue Nov 21 14:56:50 2006 Subject: [Histonet] Colored marking beads In-Reply-To: <45630E57020000C9000021B9@GHSGWIANW5V.GEISINGER.EDU> Message-ID: Nooooooo!!!! Don't go to a scientific vendor!!!! No offense, you vendors listening in, but just go to your local craft store (Michael's is one of my favorites) go to the bead section and buy bulk glass beads, size "E" in any color you desire! We use at embedding as markers to clue techs in for special instructions. I've also seen them used so that labs can permanently track which tech is embedding which cassette. We just drop them into the molten paraffin in the back of the cassette after embedding. I'm sure there are 1000 uses for all the different colors that are available! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Angela Bitting Sent: Tuesday, November 21, 2006 2:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Colored marking beads Does anyone know what vendors sell small colored beads to put into your tissue cassettes before processing? Our P.A. saw them in a flyer somewhere but he can't remember where. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. From nfournier <@t> sasktel.net Tue Nov 21 17:16:01 2006 From: nfournier <@t> sasktel.net (N Fournier) Date: Tue Nov 21 17:16:09 2006 Subject: [Histonet] need advice badly Message-ID: <3f9423b8ed18.45632641@sasktel.net> Dear Colleagues, We are having some problems with very large air bubbles being deposited along entire rat brain sections that were mounted onto you slides and would like some suggestions to fix the problem. The slides appear in good order for several weeks and then the problem emerges. On average we mount three sections or so to a slide. The bubble tends to surround the entire section for the most part sometimes, but the periphery of the bubbles do not seems to merge or ?flow together? for the most part. Nor does it seems that it flows towards the periphery of the slide. Although originally the coverslipping was performed by a junior lab member; we have also noticed this occurring with more senior members. We thought at first that it might be the mounting medium (used Entellan mounting medium originally but now switch to Cytoseal 60 since this mounting medium does not make as much of a mess); however, we have notice this occur with cytoseal mounting medium. (I thought the bottle might have been old and have subsequently replaced it. I personally used the new bottle and have had no problems?yet). I do not believe it due to an tissue thickness since the tissue was sectioned at approximately 40 to 50 microns on a vibrating microtome. Another possibility that I thought is small microcracks causing air to slowly enter the slide. This is becoming extremely annoying since it takes an extremely long time to generate the tissue for immunohistochemistry. It is also problematic for quantification. I have thought of re-coverslipping these slides by placing them in xylene for a few hours. The sections do not really look dried out and the stain still remains at least from what I can discern. Is this the best procedure to do or is this precious tissue lost for forever. (We really do not want to re-do the immunos because of all the time and money spent thus far). Is there a better technique to use? Any help is appreciated, N Fournier From llewllew <@t> shaw.ca Tue Nov 21 17:55:52 2006 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Tue Nov 21 17:56:04 2006 Subject: [Histonet] need advice badly References: <3f9423b8ed18.45632641@sasktel.net> Message-ID: <000501c70dc8$94206600$130e4246@yourlk4rlmsu> A common practice with hand mounted slides is to dip them in xylene, wipe the back, wick an edge, but leave a considerable amount of xylene over the tissue and slide. This is to facilitate the flow of mounting medium and reduce bubbles being formed as the medium flows over the slide. However, if there is too much xylene it dilutes the mounting medium, and while it makes for a nicely coverslipped section, as the xylene evaporates over time the medium contracts back, often from the tissue edge, forming a bubble. The solution is to drain almost all of the xylene from the slide before coverslipping. The tissue should be close to dry, but just moist. This involves using a fair bit more mounting medium and requires some practice as the medium flows more slowly. It is not a difficult skill to develop, however. You could also use DPX and apply a fair amount of it so that as the xylene evaporates it draws from the excess as a reservoir. After a month you can strip the excess DPX off. I prefer the first way, just be careful not to let the tissue get so devoid of xylene that it actually does dry and starts cracking. Bryan Llewellyn ----- Original Message ----- From: "N Fournier" To: Sent: Tuesday, November 21, 2006 3:16 PM Subject: [Histonet] need advice badly Dear Colleagues, We are having some problems with very large air bubbles being deposited along entire rat brain sections that were mounted onto you slides and would like some suggestions to fix the problem. The slides appear in good order for several weeks and then the problem emerges. On average we mount three sections or so to a slide. The bubble tends to surround the entire section for the most part sometimes, but the periphery of the bubbles do not seems to merge or ?flow together? for the most part. Nor does it seems that it flows towards the periphery of the slide. Although originally the coverslipping was performed by a junior lab member; we have also noticed this occurring with more senior members. We thought at first that it might be the mounting medium (used Entellan mounting medium originally but now switch to Cytoseal 60 since this mounting medium does not make as much of a mess); however, we have notice this occur with cytoseal mounting medium. (I thought the bottle might have been old and have subsequently replaced it. I personally used the new bottle and have had no problems?yet). I do not believe it due to an tissue thickness since the tissue was sectioned at approximately 40 to 50 microns on a vibrating microtome. Another possibility that I thought is small microcracks causing air to slowly enter the slide. This is becoming extremely annoying since it takes an extremely long time to generate the tissue for immunohistochemistry. It is also problematic for quantification. I have thought of re-coverslipping these slides by placing them in xylene for a few hours. The sections do not really look dried out and the stain still remains at least from what I can discern. Is this the best procedure to do or is this precious tissue lost for forever. (We really do not want to re-do the immunos because of all the time and money spent thus far). Is there a better technique to use? Any help is appreciated, N Fournier _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pex0220 <@t> yahoo.com.cn Wed Nov 22 09:36:36 2006 From: pex0220 <@t> yahoo.com.cn (pex) Date: Wed Nov 22 09:36:47 2006 Subject: [Histonet] How to distinguish the different zones in primary ossification center of newborn mouse? Message-ID: <893979.31347.qm@web15209.mail.cnb.yahoo.com> Dear all, I would like to perform LCM from legs of newborn mice. However, it is difficult to distinguish the different zones in primary ossification center of newborn mice. Does someone have some nice HE staining pictures about legs of newborn mice? It can clearly show the proliferative zone, hypertrophic zone, and calcified zone. In addition, How can i distinguish them from cell morphology? Thanks a lot. Guofeng Qian Department of anatomy and cell biology Justus-Liebig-Univeristy of giessen Aulweg 123 35392 Giessen, Germany --------------------------------- ÇÀ×¢ÑÅ»¢Ãâ·ÑÓÊÏä-3.5GÈÝÁ¿£¬20M¸½¼þ£¡ From sbanwait <@t> buckinstitute.org Wed Nov 22 12:10:15 2006 From: sbanwait <@t> buckinstitute.org (Surita Banwait) Date: Wed Nov 22 12:10:28 2006 Subject: [Histonet] (no subject) Message-ID: Hi There, Would anyone happen to know if there is a way to attach frozen tissue to cryostat chucks without using OCT media or super glue? Also, what are the 86% non-reactive ingredients in Tissue Tek OCT media? Thanks, Surita From sbanwait <@t> buckinstitute.org Wed Nov 22 12:13:45 2006 From: sbanwait <@t> buckinstitute.org (Surita Banwait) Date: Wed Nov 22 12:13:50 2006 Subject: [Histonet] OCT questions In-Reply-To: Message-ID: Hi There, Would anyone happen to know if there is a way to attach frozen tissue to cryostat chucks without using OCT media or super glue? Also, what are the 86% non-reactive ingredients in Tissue Tek OCT media? Thanks, Surita From LSebree <@t> uwhealth.org Wed Nov 22 12:23:28 2006 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Wed Nov 22 12:23:37 2006 Subject: [Histonet] OCT questions In-Reply-To: Message-ID: In a previous job, I used 5% gum tragacanth to freeze rabbit myocardium in. It cut well and orientation was quite easy as it is stiffer than OCT. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Surita Banwait Sent: Wednesday, November 22, 2006 12:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] OCT questions Hi There, Would anyone happen to know if there is a way to attach frozen tissue to cryostat chucks without using OCT media or super glue? Also, what are the 86% non-reactive ingredients in Tissue Tek OCT media? Thanks, Surita _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rennie11505 <@t> yahoo.com Wed Nov 22 13:14:02 2006 From: rennie11505 <@t> yahoo.com (Adrienne Anderson) Date: Wed Nov 22 13:14:11 2006 Subject: [Histonet] Question on calcified samples Message-ID: <20061122191402.43090.qmail@web37012.mail.mud.yahoo.com> Hello all, I am a histotechnology student, and my classmates and I will be working with some coral samples this year. I was just wondering if there was a way to prepare frozen sections on calcified samples, and if so, what equipment is needed? Thank you, Adrienne Mazzante Histotechnology Program Medical University of South Carolina __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From Kim.Shields <@t> viha.ca Wed Nov 22 13:26:52 2006 From: Kim.Shields <@t> viha.ca (Shields, Kim) Date: Wed Nov 22 13:27:18 2006 Subject: [Histonet] Used Cold Plates Message-ID: Hello, does anyone have a source for used cold plates? Thanks in advance !!! From PMonfils <@t> Lifespan.org Wed Nov 22 13:38:17 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Nov 22 13:38:31 2006 Subject: [Histonet] Question on calcified samples Message-ID: <4EBFF65383B74D49995298C4976D1D5E273BB5@LSRIEXCH1.lsmaster.lifespan.org> Hello Adrienne, When dealing with vertebrate tissues, it is possible to cut usable frozen sections of lightly to moderately calcified tissue such as cancellous bone. But it is very difficult to cut frozen sections of heavily calcified materials like cortical bone, without decalcifying it first. It usually requires special equiment that is not available too many places, like a heavy duty freezing sledge microtome. Frozen sections and paraffin sections of hard bone can be made by decalcifying the bone first (so that it isn't hard anymore). Unfortunately, with coral you don't have that option. Bone has a fibrous/cellular matrix that is infiltrated with calcium salts, so you can remove the calcium salts and still have the basic structure of the tissue. But the coral skeleton is virtually 100% calcium salts, with no organic matrix, so decalcifying it would mean completely dissolving it. The coral polyps of course would be no problem. They should section easily. There is a method by which thin sections of such hard, calcareous materials can be produced, but it is quite difficult, expensive, and requires additional special equipment. Briefly, it involves embedding the material in a hard plastic like methyl methacrylate, cutting a thin slab off the block with a motorized diamond saw, cementing the slab to a slide, and then grinding the slab down to the desired thickness with a special motorized grinding wheel and special abrasives. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > Adrienne Anderson > Sent: Wednesday, November 22, 2006 11:14 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Question on calcified samples > > Hello all, > I am a histotechnology student, and my classmates and I will be working > with some coral samples this year. I was just wondering if there was a way > to prepare frozen sections on calcified samples, and if so, what equipment > is needed? > > Thank you, > > Adrienne Mazzante > Histotechnology Program > Medical University of South Carolina > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Jessica <@t> medstaffservices.com Wed Nov 22 13:51:02 2006 From: Jessica <@t> medstaffservices.com (Jessica Hirsch) Date: Wed Nov 22 13:51:14 2006 Subject: [Histonet] Please post message in digest Message-ID: CURRENT EMPLOYMENT OPPROTUNITIES (HISTOLOGY, IMMUNOHISTOCHEMISTRY & PATHOLOGY) For immediate consideration for any position: Send resumes to Jessica@medstaffservices.com OR fax to 732.238.2152 For questions call: Jessica Hirsch at 732.238.6050 ext.58 I. Clarksburg, Maryland (Large Nationwide Clinical Labratory (division of BioReference)) 1. Histology Supervisor: Hours: Monday - Friday 12 midnight - 8:30am Pay: $25/hr + 10% night differential = $27.50/hr Benefits: Medical, Dental, 401k 2. Histology Technologist Hours: Monday - Friday 12 midnight - 8:30am Pay: $25/hr + 10% night differential = $27.50/hr Benefits: Medical, Dental, 401k 3. Grossers Hours: Monday - Friday 7pm-11:30pm Pay: $30/hr II. Elmwood Park, New Jersey ( Nationwide Clinical Laboratory) 1. Histology Technologist ( 2 positions available, immediate start ) Hours: Monday - Friday, 9:00pm - 5:30am Pay: Negotiable Benefits: Medical, Dental, 401k 2. ImmunoHistoChemistry Supervisor Hours: Monday - Friday, 7am or 8am - 4pm or 5pm Pay: Negotiable Benefits: Medical, Dental, 401k III. New York City, New York ( large world renowned Cancer Hospital ) 1. Pathologist Assistant Hours: Monday - Friday, Day shift, Full-Time Pay: $60k - $80k based on experience Benefits: Major Medical, Dental, Vision, 401k, etc. 2. Histology Technologist Hours: Monday - Friday (shift to be announced) Pay: negotiable Benefits: Medical, Dental, 401k For immediate consideration for any position: Send resumes to Jessica@medstaffservices.com OR fax to 732.238.2152 For questions call: Jessica Hirsch at 732.238.6050 ext.58 From Jessica <@t> medstaffservices.com Wed Nov 22 14:08:38 2006 From: Jessica <@t> medstaffservices.com (Jessica Hirsch) Date: Wed Nov 22 14:08:48 2006 Subject: [Histonet] Please post message Message-ID: CURRENT EMPLOYMENT OPPROTUNITIES (HISTOLOGY, IMMUNOHISTOCHEMISTRY & PATHOLOGY) For immediate consideration for any position: Send resumes to Jessica@medstaffservices.com OR fax to 732.238.2152 For questions call: Jessica Hirsch at 732.238.6050 ext.58 I. Clarksburg, Maryland (Large Nationwide Clinical Labratory (division of BioReference)) 1. Histology Supervisor: Hours: Monday - Friday 12 midnight - 8:30am Pay: $27.50 - $30 /hr Benefits: Medical, Dental, 401k 2. Histology Technologist Hours: Monday - Friday 12 midnight - 8:30am Pay: $ 21-$23 hr + 10% night differential Benefits: Medical, Dental, 401k 3. Grossers Hours: Monday - Friday 7pm-11:30pm Pay: $30/hr II. Elmwood Park, New Jersey ( Nationwide Clinical Laboratory) 1. Histology Technologist ( 2 positions available, immediate start ) Hours: Monday - Friday, 9:00pm - 5:30am Pay: Negotiable Benefits: Medical, Dental, 401k 2. ImmunoHistoChemistry Supervisor Hours: Monday - Friday, 7am or 8am - 4pm or 5pm Pay: Negotiable Benefits: Medical, Dental, 401k III. New York City, New York ( large world renowned Cancer Hospital ) 1. Pathologist Assistant Hours: Monday - Friday, Day shift, Full-Time Pay: $60k - $80k based on experience Benefits: Major Medical, Dental, Vision, 401k, etc. 2. Histology Technologist Hours: Monday - Friday (shift to be announced) Pay: negotiable Benefits: Medical, Dental, 401k For immediate consideration for any position: Send resumes to Jessica@medstaffservices.com OR fax to 732.238.2152 For questions call: Jessica Hirsch at 732.238.6050 ext.58 From abright <@t> brightinstruments.com Thu Nov 23 08:26:36 2006 From: abright <@t> brightinstruments.com (Alan Bright) Date: Thu Nov 23 08:26:36 2006 Subject: [Histonet] OCT questions Message-ID: Dear Surita, Yes use water to attach your tissue, this was the main method used when we first started manufacturing cryostats in the 50's. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype User ID: dazzle0 -----Original Message----- From: Surita Banwait [mailto:sbanwait@buckinstitute.org] Sent: 22 November 2006 18:14 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] OCT questions Hi There, Would anyone happen to know if there is a way to attach frozen tissue to cryostat chucks without using OCT media or super glue? Also, what are the 86% non-reactive ingredients in Tissue Tek OCT media? Thanks, Surita _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Nov 23 08:53:38 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Nov 23 08:53:50 2006 Subject: [Histonet] OCT questions In-Reply-To: Message-ID: <20061123145338.21618.qmail@web61220.mail.yahoo.com> Dear Surita: As standard procedure for direct immunofluorescence I always used distilled water to do skin frozen sections. The cryostat has to be at least at -20?C and you addd a large drop, place the specimen inside it and keep building more ice around it as it freezes. Nothing to wash afterwards! Ren? J. Alan Bright wrote: Dear Surita, Yes use water to attach your tissue, this was the main method used when we first started manufacturing cryostats in the 50's. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype User ID: dazzle0 -----Original Message----- From: Surita Banwait [mailto:sbanwait@buckinstitute.org] Sent: 22 November 2006 18:14 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] OCT questions Hi There, Would anyone happen to know if there is a way to attach frozen tissue to cryostat chucks without using OCT media or super glue? Also, what are the 86% non-reactive ingredients in Tissue Tek OCT media? Thanks, Surita _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Want to start your own business? Learn how on Yahoo! Small Business. From pex0220 <@t> yahoo.com.cn Thu Nov 23 11:36:07 2006 From: pex0220 <@t> yahoo.com.cn (docqian) Date: Thu Nov 23 11:36:20 2006 Subject: [Histonet] Cut frozen bone sections Message-ID: <20061123173607.84861.qmail@web15208.mail.cnb.yahoo.com> Dear all,=0A=0AI would like to cut frozen bone sections without decalcifica= tion, samples are from knees of 4w mouse. Today I try to cut them, however,= the sections are broken. So I can not see the complete bone structure unde= r microscopy.=0AIf someone has some experience about it. please do me a fav= or. Thanks a lot=0A=0A=0AGuofeng Qian=0A=0ADepartment of anatomy and cell b= iology=0AJustus-Liebig-University of Giessen=0AAulweg 123=0A35392 Giessen, = Germany=0A=0A=0A=09=09=0A__________________________________________________= _________ =0A=D1=C5=BB=A2=C3=E2=B7=D1=D3=CA=CF=E4-3.5G=C8=DD=C1=BF=A3=AC20M= =B8=BD=BC=FE =0Ahttp://cn.mail.yahoo.com/From jpastor1 <@t> nycap.rr.com Thu Nov 23 13:23:02 2006 From: jpastor1 <@t> nycap.rr.com (joseph pastore) Date: Thu Nov 23 13:16:06 2006 Subject: [Histonet] Cut frozen bone sections (docqian) Message-ID: <002a01c70f34$cbb5bdf0$7864a8c0@JGPONLINE> Hi, A number of years ago I used a technique for frozen sections of undecalcified bone. It involved using a special tape that is affixed to the face of the block.. As the section is cut it adheres to the tape. The tape is then affixed to a slide coated with a UV activated adhesive and the slide with section attached is placed under a UV lamp. It did require practice but the sections were excelent. All the materials including the lamp came in a kit. Unfotunately I don't remember the source. You might try Electron Microscopy Supply or a specilty histology company. From David.Muskett <@t> RLC.NHS.UK Fri Nov 24 02:15:57 2006 From: David.Muskett <@t> RLC.NHS.UK (Muskett David) Date: Fri Nov 24 02:15:57 2006 Subject: [Histonet] Unsubscribe Message-ID: Unsubscribe Thank you David Muskett Chief Biomedical Scientist, Histology Royal Liverpool Children's NHS Trust Eaton Road Liverpool, UK L12 2AP Telephone (0151) 293 3656 Fax (0151) 293 3617 http://www.alderhey.com/RLCH/Laboratory_Medicine.asp From pmarcum <@t> vet.upenn.edu Fri Nov 24 12:48:46 2006 From: pmarcum <@t> vet.upenn.edu (pmarcum@vet.upenn.edu) Date: Fri Nov 24 12:48:55 2006 Subject: [Histonet] Hexane freezing Method for Cryosectioning Message-ID: <1164394126.45673e8eda828@imp.vet.upenn.edu> Good Afternoon, I have been asked to look into using hexane in rapid freezing of tissues (primarily non-decalcified bone). I am looking it up with several other searches and wondered if anyone is currently using this technique. Is it better or safer than isopentane with liquid nitrogen? I know the issues with isopentane and explosions so this partly a safety questions also. Thanks for the help in advance. Pam Marcum UPENN Vet School New Bolton Center From splaza <@t> tylerpathology.com Fri Nov 24 13:01:19 2006 From: splaza <@t> tylerpathology.com (Susan Plaza) Date: Fri Nov 24 13:09:30 2006 Subject: [Histonet] Re: Tape for cutting undecalcified bone frozens Message-ID: <000001c70ffa$efac0fe0$800aa8c0@domain.local> -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 36, Issue 29 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu Hi, Andy Mena with Stat Lab (800-442-3573 x224)recently showed us a tape that would do what you described except on fatty or unfixed paraffin sections. It uses an ultra violet light also. Try giving him a call. He may be able to help you. You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Cut frozen bone sections (docqian) (joseph pastore) 2. Unsubscribe (Muskett David) ---------------------------------------------------------------------- Message: 1 Date: Thu, 23 Nov 2006 14:23:02 -0500 From: "joseph pastore" Subject: [Histonet] Cut frozen bone sections (docqian) To: Message-ID: <002a01c70f34$cbb5bdf0$7864a8c0@JGPONLINE> Content-Type: text/plain; charset="iso-8859-1" Hi, A number of years ago I used a technique for frozen sections of undecalcified bone. It involved using a special tape that is affixed to the face of the block.. As the section is cut it adheres to the tape. The tape is then affixed to a slide coated with a UV activated adhesive and the slide with section attached is placed under a UV lamp. It did require practice but the sections were excelent. All the materials including the lamp came in a kit. Unfotunately I don't remember the source. You might try Electron Microscopy Supply or a specilty histology company. ------------------------------ Message: 2 Date: Fri, 24 Nov 2006 08:15:57 -0000 From: "Muskett David" Subject: [Histonet] Unsubscribe To: Message-ID: Content-Type: text/plain; charset="us-ascii" Unsubscribe Thank you David Muskett Chief Biomedical Scientist, Histology Royal Liverpool Children's NHS Trust Eaton Road Liverpool, UK L12 2AP Telephone (0151) 293 3656 Fax (0151) 293 3617 http://www.alderhey.com/RLCH/Laboratory_Medicine.asp ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 36, Issue 29 **************************************** From pruegg <@t> ihctech.net Fri Nov 24 15:19:36 2006 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Fri Nov 24 15:19:54 2006 Subject: [Histonet] Re: Tape for cutting undecalcified bone frozens In-Reply-To: <000001c70ffa$efac0fe0$800aa8c0@domain.local> Message-ID: <200611242119.kAOLJfOi011462@pro12.abac.com> The tape transfer system comes from Instrumedics which has been bought by McCormick but I believe they still operate under the Instrumedics name. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Susan Plaza Sent: Friday, November 24, 2006 12:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Tape for cutting undecalcified bone frozens -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 36, Issue 29 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu Hi, Andy Mena with Stat Lab (800-442-3573 x224)recently showed us a tape that would do what you described except on fatty or unfixed paraffin sections. It uses an ultra violet light also. Try giving him a call. He may be able to help you. You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Cut frozen bone sections (docqian) (joseph pastore) 2. Unsubscribe (Muskett David) ---------------------------------------------------------------------- Message: 1 Date: Thu, 23 Nov 2006 14:23:02 -0500 From: "joseph pastore" Subject: [Histonet] Cut frozen bone sections (docqian) To: Message-ID: <002a01c70f34$cbb5bdf0$7864a8c0@JGPONLINE> Content-Type: text/plain; charset="iso-8859-1" Hi, A number of years ago I used a technique for frozen sections of undecalcified bone. It involved using a special tape that is affixed to the face of the block.. As the section is cut it adheres to the tape. The tape is then affixed to a slide coated with a UV activated adhesive and the slide with section attached is placed under a UV lamp. It did require practice but the sections were excelent. All the materials including the lamp came in a kit. Unfotunately I don't remember the source. You might try Electron Microscopy Supply or a specilty histology company. ------------------------------ Message: 2 Date: Fri, 24 Nov 2006 08:15:57 -0000 From: "Muskett David" Subject: [Histonet] Unsubscribe To: Message-ID: Content-Type: text/plain; charset="us-ascii" Unsubscribe Thank you David Muskett Chief Biomedical Scientist, Histology Royal Liverpool Children's NHS Trust Eaton Road Liverpool, UK L12 2AP Telephone (0151) 293 3656 Fax (0151) 293 3617 http://www.alderhey.com/RLCH/Laboratory_Medicine.asp ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 36, Issue 29 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Fri Nov 24 15:25:46 2006 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Fri Nov 24 15:26:01 2006 Subject: [Histonet] Hexane freezing Method for Cryosectioning In-Reply-To: <1164394126.45673e8eda828@imp.vet.upenn.edu> Message-ID: <200611242125.kAOLPpeA013674@pro12.abac.com> Pam, Hexane is still very flammable, not sure if it is any less explosive, both would probably be considered pretty hazardous. If you are using liquid nitrogen why do you also need the solvent? I snap freeze by putting oct around the sample in a cryo mold, then slowly lower into liquid nitrogen with good results for frozen sections on undecalcified bone, better yet for tape transfer sections, I fix, wash and infiltrate over night at 4dc in 30% sucrose, then do the snap freezing with OCT and liquid nitrogen. John Trapley wrote an article for JOH on this several years ago. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pmarcum@vet.upenn.edu Sent: Friday, November 24, 2006 11:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hexane freezing Method for Cryosectioning Good Afternoon, I have been asked to look into using hexane in rapid freezing of tissues (primarily non-decalcified bone). I am looking it up with several other searches and wondered if anyone is currently using this technique. Is it better or safer than isopentane with liquid nitrogen? I know the issues with isopentane and explosions so this partly a safety questions also. Thanks for the help in advance. Pam Marcum UPENN Vet School New Bolton Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From micro <@t> superlink.net Fri Nov 24 20:41:42 2006 From: micro <@t> superlink.net (Markus F. Meyenhofer) Date: Fri Nov 24 20:41:58 2006 Subject: [Histonet] Hexane freezing Method for Cryosectioning References: <200611242125.kAOLPpeA013674@pro12.abac.com> Message-ID: <02e801c7103b$3e4829a0$9b893cd1@DJ4VDH31> Why solvents? To counter the "Leidenfrost Effect" . Google or wikipedia for the explanation> Markus ----- Original Message ----- From: "patsy ruegg" To: ; Sent: Friday, November 24, 2006 4:25 PM Subject: RE: [Histonet] Hexane freezing Method for Cryosectioning > Pam, > Hexane is still very flammable, not sure if it is any less explosive, both > would probably be considered pretty hazardous. If you are using liquid > nitrogen why do you also need the solvent? I snap freeze by putting oct > around the sample in a cryo mold, then slowly lower into liquid nitrogen > with good results for frozen sections on undecalcified bone, better yet > for > tape transfer sections, I fix, wash and infiltrate over night at 4dc in > 30% > sucrose, then do the snap freezing with OCT and liquid nitrogen. John > Trapley wrote an article for JOH on this several years ago. > Patsy > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > pmarcum@vet.upenn.edu > Sent: Friday, November 24, 2006 11:49 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Hexane freezing Method for Cryosectioning > > Good Afternoon, > > I have been asked to look into using hexane in rapid freezing of tissues > (primarily non-decalcified bone). I am looking it up with several other > searches and wondered if anyone is currently using this technique. Is it > better or safer than isopentane with liquid nitrogen? I know the issues > with > isopentane and explosions so this partly a safety questions also. > > Thanks for the help in advance. > > Pam Marcum > UPENN Vet School > New Bolton Center > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tkngflght <@t> yahoo.com Fri Nov 24 23:05:53 2006 From: tkngflght <@t> yahoo.com (Cheryl) Date: Sat Nov 25 00:06:07 2006 Subject: [Histonet] Help--still needing a grossing histotech?! In-Reply-To: <3996EDB4-DB02-4609-8A06-6B190A4171A2@mac.com> Message-ID: <574784.91226.qm@web50905.mail.yahoo.com> Hi All~ I think I've exhausted all of my resources! I'm in need of a grossing Histotech and can't find one for a temp assignment--I'm going to probably work at this lab for a week or two so we have more time to find someone who is a good fit and might want the remaining 6 to 10 weeks....do you or does anyone you know want a job that pays well, has decent hours and will facilitate as many weeks off as you want off before you head back to work? If you are interested, please call my cell 281.882.7704, or ring the 800 that transfers to my cell: 800.756.3309. The techs who work with us will tell you--we're fair and keep our promises--and then some. Referral bonuses apply--I hope to hear from you!! Cheryl Kerry, HT(ASCP) Full Staff Inc. 281.852.9457 ancillarypath@mac.com wrote: Dear Colleagues, This is Hadi Yaziji. I am the 'presenter' who gave the FISH course at the NSH meeting and I apologize for all of you who waited patiently to receive the CD. I think there was a major misunderstanding on my part: I thought that people who are interested in the file will email me to give me instructions how to send the CD. Those of you who are interested in the CD (like Glen), can you please email me back (privately) so I can actually send you login and password information to download the pdf file from our ftp server? This way you will save my office people a lot of time burning and mailing the CDs. Those of you who still want the CD will get it, but please indicate your preference and include a physical address in your reply email. Thank you for understanding and my sincere apologies to those who've been patiently waiting. Best regards, Hadi Yaziji, M.D. President, Ancillary Pathways, LLC. 7000 SW 62nd Ave, Suite PH-C Miami, FL 33143 Phone: 305.740.4440 Cell: 786.266.5725 Fax: 786-513-0175 www.ancillarypath.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sat Nov 25 12:40:08 2006 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Sat Nov 25 12:40:27 2006 Subject: [Histonet] Hexane freezing Method for Cryosectioning In-Reply-To: <02e801c7103b$3e4829a0$9b893cd1@DJ4VDH31> Message-ID: <200611251840.kAPIeDvd058519@pro12.abac.com> I did look up Leidenfrost Effect which to me says that the droplet dancing effect is less with solvents than with water media (OCT), but my experience tells me that if I slowly lower a mold of OCT surrounded tissue into liquid nitrogen I do not experience any detritus effects on the tissue, I think the idea is to freeze the tissue fast enough to minimize crystal forming freezing artifact (this is never completely absent, but with care the crystals can be so small as not to affect the cell morphology)but not to just plop the mold into LN so that it would dance around so violently to disturb the tissue morphology. Just my antidotal experience here. I have a lot of experience with freezing undecalcified bone for tape transfer sectioning and this method has served me well. Patsy -----Original Message----- From: Markus F. Meyenhofer [mailto:micro@superlink.net] Sent: Friday, November 24, 2006 7:42 PM To: patsy ruegg; pmarcum@vet.upenn.edu; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Hexane freezing Method for Cryosectioning Why solvents? To counter the "Leidenfrost Effect" . Google or wikipedia for the explanation> Markus ----- Original Message ----- From: "patsy ruegg" To: ; Sent: Friday, November 24, 2006 4:25 PM Subject: RE: [Histonet] Hexane freezing Method for Cryosectioning > Pam, > Hexane is still very flammable, not sure if it is any less explosive, both > would probably be considered pretty hazardous. If you are using liquid > nitrogen why do you also need the solvent? I snap freeze by putting oct > around the sample in a cryo mold, then slowly lower into liquid nitrogen > with good results for frozen sections on undecalcified bone, better yet > for > tape transfer sections, I fix, wash and infiltrate over night at 4dc in > 30% > sucrose, then do the snap freezing with OCT and liquid nitrogen. John > Trapley wrote an article for JOH on this several years ago. > Patsy > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > pmarcum@vet.upenn.edu > Sent: Friday, November 24, 2006 11:49 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Hexane freezing Method for Cryosectioning > > Good Afternoon, > > I have been asked to look into using hexane in rapid freezing of tissues > (primarily non-decalcified bone). I am looking it up with several other > searches and wondered if anyone is currently using this technique. Is it > better or safer than isopentane with liquid nitrogen? I know the issues > with > isopentane and explosions so this partly a safety questions also. > > Thanks for the help in advance. > > Pam Marcum > UPENN Vet School > New Bolton Center > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jysho <@t> hkusua.hku.hk Sun Nov 26 01:38:42 2006 From: jysho <@t> hkusua.hku.hk (Janice Ho) Date: Sun Nov 26 01:39:07 2006 Subject: [Histonet] need a protocol for whole rat brain tissue processing Message-ID: <1164526722.45694482cc1f9@imp.webmail.hku.hk> Hello, I have some sample of rat brain which is not perfused with any fixitive before. The tissue is profixed in 4% paraformaldehyde for a week and then processed for paraffin section. But when the section is cut, it expands and breaks when float in water. Does anyone know why this happen and can give suggestion for the protocol? I also have rat brain when received 4% PFA perfucion before dissected out. These whole rat brain is profixed in fixitive overnight and then dehydrated with alcohol, again, after xylene and wex embedding, it breaks up when cut into 8 micron section. Does anyone can give me some suggestions on the protocol? Thanks a lot. Janice Ho University of Hong Kong From adisabag <@t> techunix.technion.ac.il Mon Nov 27 02:25:01 2006 From: adisabag <@t> techunix.technion.ac.il (Adi Sabag) Date: Mon Nov 27 02:25:13 2006 Subject: [Histonet] (no subject) Message-ID: <1164615901.456aa0dd87e41@webmail.technion.ac.il> Hello, I'm a PhD student and recently started working with cryo sections. I'm working with s.c tumors that are approximately 8x8 mm, they were fixed with 4% PFA and then either frozen in isopentene over liquid N2 or I used a protocol of embedding in sucrose and then freezing. In both cases I found that the optimal temperature for getting nice sections is around 20C. I'm using 3 different sizes of sections- 60, 30 and 10um. The problem is that when I'm trying to pick up the sample from the stage it adheres to the slide from the margins toward the center of the tumor and at the very center it does not adhere. This region remains unstuck to the slides and after a few minutes it starts breaking and a hole is created in the middle of the tissue which gets bigger with time. After a while the section is almost useless. If anyone have an idea on how to deal with this problem it will be a great help! Thanks- Adi -- Adi Sabag Tumor Progression and Angiogenesis laboratory Medicine Faculty, Technion Haifa, Israel. 972-4-8295425 From stern <@t> ipmc.cnrs.fr Mon Nov 27 05:00:41 2006 From: stern <@t> ipmc.cnrs.fr (Alejandro Ortiz Stern) Date: Mon Nov 27 04:55:31 2006 Subject: [Histonet] A good anti mouse anti smooth muscle mAb Message-ID: <456AC559.6030102@ipmc.cnrs.fr> Hello histoneters, I am looking for a good anti mouse anti smooth muscle mAb that is suitable for IHC or Fluorescent staining for confocal imaging. If anybody has experience with one such antibody please let me know, or if you know of antibodies that do NOT work, that is also valuable information. Thank you very much in advance. Alejandro -- Alejandro Ortiz Stern M.D. Ph.D., Postdoctoral Fellow Institut National de la Sant? et de la Recherche M?dicale, E0344 Universit? de Nice-Sophia-Antipolis Institut de Pharmacologie Mol?culaire et Cellulaire 660 Route des Lucioles - 06560 Valbonne - FRANCE TEL: 33 (4) 93 95 77 80 - FAX: 33 (4) 93 95 77 08 From j.lydon <@t> neurotechusa.com Mon Nov 27 06:40:55 2006 From: j.lydon <@t> neurotechusa.com (John Lydon) Date: Mon Nov 27 06:41:04 2006 Subject: [Histonet] FS: Stainless slide boxes Message-ID: Good morning Histonetters- My lab has recently changed the way we store slides, so.... I have approximately 50 Shandon Stainless slide boxes, part#1210 to get rid of. They are in good shape, with some scratches, dings, etc. $20 each, or make us an offer. (Cost is $60 normally) If you've never seen these boxes before, each box holds 240 slides. We also have 5 of the Shandon slide cabinets. Each cabinet holds 10 of the slide boxes mentioned above. Part #12003. Make us an offer, retails for $310. We need to get rid of them to free up lab space. Please email me directly off line, as I don't want to clog up everyone's mailboxes with unnecessary messages. j.lydon@neurotechusa.com Thanks. John From anh2006 <@t> med.cornell.edu Mon Nov 27 07:15:02 2006 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Mon Nov 27 07:15:16 2006 Subject: [Histonet] A good anti mouse anti smooth muscle mAb In-Reply-To: <456AC559.6030102@ipmc.cnrs.fr> References: <456AC559.6030102@ipmc.cnrs.fr> Message-ID: Clone 1A4 mouse anti-human SMA works beautifully on mouse tissue. It's best to buy a conjugated version though so you don't have to deal with mouse on mouse issues. At 12:00 PM +0100 11/27/06, Alejandro Ortiz Stern wrote: >Hello histoneters, > >I am looking for a good anti mouse anti smooth >muscle mAb that is suitable for IHC or >Fluorescent staining for confocal imaging. > >If anybody has experience with one such antibody >please let me know, or if you know of antibodies >that do NOT work, that is also valuable >information. > >Thank you very much in advance. > >Alejandro > >-- >Alejandro Ortiz Stern M.D. Ph.D., >Postdoctoral Fellow >Institut National de la Sant? et de la Recherche M?dicale, E0344 >Universit? de Nice-Sophia-Antipolis >Institut de Pharmacologie Mol?culaire et Cellulaire >660 Route des Lucioles - 06560 Valbonne - FRANCE >TEL: 33 (4) 93 95 77 80 - FAX: 33 (4) 93 95 77 08 -- From slappycraw <@t> yahoo.com Mon Nov 27 09:13:43 2006 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Mon Nov 27 09:13:55 2006 Subject: [Histonet] Phospho-Histone-3 and Caspace 3 on decalcified bone Message-ID: <173699.42755.qm@web53601.mail.yahoo.com> Has anyone out there done Phospho-Histone 3 and/or Caspace 3 on decalcified bone fixed in formalin and had positive success? What were your AR methods ect. Thanks. --------------------------------- Cheap Talk? Check out Yahoo! Messenger's low PC-to-Phone call rates. From mcauliff <@t> umdnj.edu Mon Nov 27 09:25:44 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Mon Nov 27 09:25:08 2006 Subject: [Histonet] need a protocol for whole rat brain tissue processing In-Reply-To: <1164526722.45694482cc1f9@imp.webmail.hku.hk> References: <1164526722.45694482cc1f9@imp.webmail.hku.hk> Message-ID: <456B0378.7010308@umdnj.edu> Hi Janice: You see what happens when you try to process an entire rat brain for paraffin embedding, the tissue is too thick to get good results. Reagents cannot penetrate in the time given. 1. Forget about immersion fixation of the whole rat brain if you want good morphology in the deeper parts of the brain, you will never get good results because the fixative can't penetrate and preserve morphology before autolysis starts. 2. Even a brain well fixed by perfusion followed by immersion in fixative for a minimum of 48 hours (one week is much better for formalin fixation) can't be processed whole as I mentioned above. Cut the brain into smaller pieces, 5-8 mm cross sections. 3. If you insist on processing the brain whole you might look into embedding with nitrocellulose but this is very time-consuming and you will probably need a sliding microtome to cut the brain. Geoff Janice Ho wrote: >Hello, > >I have some sample of rat brain which is not perfused with any fixitive before. >The tissue is profixed in 4% paraformaldehyde for a week and then processed for >paraffin section. But when the section is cut, it expands and breaks when float >in water. Does anyone know why this happen and can give suggestion for the >protocol? I also have rat brain when received 4% PFA perfucion before dissected >out. These whole rat brain is profixed in fixitive overnight and then >dehydrated with alcohol, again, after xylene and wex embedding, it breaks up >when cut into 8 micron section. Does anyone can give me some suggestions on the >protocol? Thanks a lot. > >Janice Ho >University of Hong Kong > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From slappycraw <@t> yahoo.com Mon Nov 27 09:49:52 2006 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Mon Nov 27 09:50:02 2006 Subject: [Histonet] Phospho-Histone 3 and Caspace 3 Message-ID: <20061127154953.22370.qmail@web53607.mail.yahoo.com> Has anyone out there done Phospho-Histone 3 and/or Caspace 3 on decalcified formalin fixed Mouse Tissue and had positive staining results? What were your AR methods, ect. Thanks --------------------------------- Everyone is raving about the all-new Yahoo! Mail beta. From amh-histolab <@t> amh.org Mon Nov 27 09:55:20 2006 From: amh-histolab <@t> amh.org (AMH-HistoLab) Date: Mon Nov 27 09:50:05 2006 Subject: [Histonet] trying to reconfirm, but not letting me. Message-ID: I received a message to reconfirm the message I sent about listing on histonet. the message came back to me to confirm but I guess because of the weekend it expired in 3 days. Can you please resubmit my request. the subject number I was given is: 5a7d2df747ce67b4f51e4f3154a904e57300c226 Thank you, Christina at Abington Hospital ****************** CONFIDENTIALITY NOTICE ********************** This e-mail contains LEGALLY PRIVILEGED AND CONFIDENTIAL INFORMATION intended only for the use of the recipient named above. If you are not the intended recipient, you are hereby notified that any dissemination or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please notify the transmitting hospital by telephone or e-mail and delete the original e-mail received in error. THIS INFORMATION HAS BEEN DISCLOSED TO YOU FROM RECORDS WHOSE CONFIDENTIALITY IS PROTECTED BY STATE AND FEDERAL LAW. ANY FURTHER DISCLOSURE, COPYING, DISTRIBUTION OR ACTION TAKEN IN RELIANCE ON THE CONTENTS OF THESE DOCUMENTS WITHOUT THE PRIOR WRITTEN CONSENT OF THE PERSON TO WHOM IT PERTAINS IS PROHIBITED. YOU ARE REQUIRED TO DESTROY THE INFORMATION AFTER THE STATED NEED HAS BEEN FULFILLED. From immrstambo <@t> hotmail.com Mon Nov 27 11:01:35 2006 From: immrstambo <@t> hotmail.com (Chistine Tambasco) Date: Mon Nov 27 11:01:58 2006 Subject: [Histonet] automated immuno stainer Message-ID: Hello all! I hope everyone enjoyed their breif repreive from stress. I was wondering if anyone could give me some input on automated stainers for immunohistochemistry. Currently we hand stain and our volumes are steadily increasing. We are looking at automation as we probably do up to 12-15 stains per day and plan on starting ER and PR. I heard Vantana is very good. How about Leica? (Theirs does both immunos and ordianry special stains.) Anyone have any experience they would like to share? We are an Ascension Health affiliated hospital as that may matter in the negotiations. Thanks for any and all opinions. Respectfullly, Christine Tambasco, HT (ASCP) St. Mary's Hospital Amsterdam, New York 12010 518-841-7287 From godsgalnow <@t> aol.com Mon Nov 27 11:20:58 2006 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Mon Nov 27 11:21:35 2006 Subject: [Histonet] automated immuno stainer In-Reply-To: References: Message-ID: <8C8E05F89EF3B5A-8F8-1F06@FWM-D34.sysops.aol.com> Christine, Stainers are a matter of preference and what you want to be able to do with them. The Ventanna is great if you just want to cut the slide and go bevcause it will do everything for you on-line, but it is a closed system. Dako is an open system which gives you more control, but it only can do 48 slides at a time and by the time you get your positives, negatives and patients on you may have to do 2 runs (or get 2 machines). Biocare has a IHC stainer, the Nemesis, that is just like the DAKO but can handle 84 slides at a time, this is the one I currently am using. I have used all 3 and they all work fine, but for volume, consistancy, and the ability to do triple stains and cocktails and such, the Nemesis was my chouice and have been extrememly pleased. Please no hate mail....these views are only my opinion and I represent no individual vendor, as I said I have used all of them and they all work. Roxanne Soto HT(QIHC) Lab Manager Physicians RightPath Tampa, Florida -----Original Message----- From: immrstambo@hotmail.com To: Histonet@lists.utsouthwestern.edu Sent: Mon, 27 Nov 2006 12:01 PM Subject: [Histonet] automated immuno stainer Hello all! I hope everyone enjoyed their breif repreive from stress. I was wondering if anyone could give me some input on automated stainers for immunohistochemistry. Currently we hand stain and our volumes are steadily increasing. We are looking at automation as we probably do up to 12-15 stains per day and plan on starting ER and PR. I heard Vantana is very good. How about Leica? (Theirs does both immunos and ordianry special stains.) Anyone have any experience they would like to share? We are an Ascension Health affiliated hospital as that may matter in the negotiations. Thanks for any and all opinions. Respectfullly, Christine Tambasco, HT (ASCP) St. Mary's Hospital Amsterdam, New York 12010 518-841-7287 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From rjbuesa <@t> yahoo.com Mon Nov 27 11:22:29 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Nov 27 11:22:40 2006 Subject: [Histonet] automated immuno stainer In-Reply-To: Message-ID: <20061127172229.87458.qmail@web61212.mail.yahoo.com> Christine: I have used Ventana BenchMark and Dako. In spite of the fact that Ventane is a "walk away" instrument, I prefer Dako. It is much more versatil, accepts any detection system and is simpler. Ren? J. Chistine Tambasco wrote: Hello all! I hope everyone enjoyed their breif repreive from stress. I was wondering if anyone could give me some input on automated stainers for immunohistochemistry. Currently we hand stain and our volumes are steadily increasing. We are looking at automation as we probably do up to 12-15 stains per day and plan on starting ER and PR. I heard Vantana is very good. How about Leica? (Theirs does both immunos and ordianry special stains.) Anyone have any experience they would like to share? We are an Ascension Health affiliated hospital as that may matter in the negotiations. Thanks for any and all opinions. Respectfullly, Christine Tambasco, HT (ASCP) St. Mary's Hospital Amsterdam, New York 12010 518-841-7287 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Want to start your own business? Learn how on Yahoo! Small Business. From slappycraw <@t> yahoo.com Mon Nov 27 11:57:25 2006 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Mon Nov 27 11:57:38 2006 Subject: [Histonet] automated immuno stainer Message-ID: <14610.68531.qm@web53613.mail.yahoo.com> The Dako and the Nemisis are both made by Lab Vision which makes a 36,48, and 84 slide capacity machine. The Nemisis is distributed by biocare but not for too much longer because they no longer have the rights to it because of Thermo-Shandon buying out Fisher who bought out Lab Vision. Now if you can follow that then it comes down to a closed system or an open one but biocre does not make an IHC stainer and Dako does but it's called the Eridan and it has a lot of bugs in it at the present time but has the potential to be a great instrument when it gets worked out. The Lab Vision platform which is distributed by Dako(48) and Lab Vision (36) and (84) are the open systems. Biocare claims that when they run out of the platform that they have they will replace it with a machine that they are going to build. Ventana is the closed system. There are a few others but it does really come down to open and closed and service. I personally like the open system but the closed system has it's niche as well. __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From gcallis <@t> montana.edu Mon Nov 27 12:04:30 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Nov 27 12:04:45 2006 Subject: [Histonet] Snap freezing undecalcified bone using hexane, OCT ingredients Message-ID: <6.0.0.22.1.20061127103418.01b0cbe8@gemini.msu.montana.edu> We do this all the time, and it can be done two ways. RA Dodds was one of the first who used this along with van Noorden. Dodds published in J of Histotechnology. Check out the following references for more on snap freezing bone, particularly the van Noorden paper. Hexane is one solvent used and it seems to be a gentler but thorough snap freezing. There is extensive discussion about snap freezing bone and the various solvents in Histonet archives. JR Connor, RA Dodds, IE James, and M Gowen Human osteoclast and giant cell differentiation: the apparent switch from nonspecific esterase to tartrate resistant acid phosphatase activity coincides with the in situ expression of osteopontin mRNA J. Histochem. Cytochem., Dec 1995; 43: 1193 - 1201. RA Dodds, K Merry, A Littlewood, and M Gowen Expression of mRNA for IL1 beta, IL6 and TGF beta 1 in developing human bone and cartilage J. Histochem. Cytochem., Jun 1994; 42: 733 - 744. CJ Van Noorden, IM Vogels, and RE Smith Localization and cytophotometric analysis of cathepsin B activity in unfixed and undecalcified cryostat sections of whole rat knee joints J. Histochem. Cytochem., May 1989; 37: 617 - 624. Dodds dipped undecalcified bone in a water soluble 30,000 - 70,000 MW polyvinyl alcohol to coat the bone, and then snap froze in a mixture of dry ice and hexane (pieces of dry ice in the hexane itself). We have done this but coated the bone with diluted OCT (1:1 OCT 1:1 with water since the OCT contains polyvinyl alcohol. Dodds never explained why coating was important unless to protect the surface of the bone from some of the effects of the solvent. The frozen bone could then be attached to the metal disk with either water, OCT, or some other cryoembedding media (2% methyl cellulose is a super hard hold for bone). OCT contains polyvinyl alcohol, polyethylene glycol and water. The molecular weights of these chemical are given, a proprietary issue, but we use it for undecalcified bone cryotomy along with the Instrumedics Cryojane tape transfer system and have good results. We prefer to embed the bone in OCT, then snap freeze with the hexane/dry ice mixture. Hexane is explosive, and not a good thing to breathe in. Fume hoods should be used and some use respirators to protect from hexane fumes. One thing that can happen with liquid nitrogen snap freezing is bone shatters or splits. We experienced this with immature bovine bone and also delicate nasal bones on a mouse head which will split apart. The liquid nitrogen temperature may be too cold for this calcified matrix but hexane/dry ice freezing prevents this shattering. Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 From MElliott <@t> mrl.ubc.ca Mon Nov 27 14:01:35 2006 From: MElliott <@t> mrl.ubc.ca (Mark Elliott) Date: Mon Nov 27 14:03:08 2006 Subject: [Histonet] Endothelium, laminin and collagen on rabbit tissue Message-ID: <456AD39F020000D60002BE11@mail.mrl.ubc.ca> Good day to you all I have been asked to stain some formalin fixed paraffin embedded rabbit lungs with antibodies to the above 3 molecules/cell types. I was wondering what people are using for these. Since these are rabbit tissue I want to avoid polyclonals raised in rabbits obviously. The researcher wants to demonstrate all endothelial cells-is vWF the best, or is there some other marker which works better? Any infomation greatly appreciated. Thanks for your input. Mark in very snowy Vancouver, Canada ***CONFIDENTIALITY NOTICE*** This electronic message is intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From FLabrador <@t> lifecell.com Mon Nov 27 14:11:48 2006 From: FLabrador <@t> lifecell.com (Frances Labrador) Date: Mon Nov 27 14:12:00 2006 Subject: [Histonet] please unsubscribe me from list Message-ID: *********************************************************************************************************************************************** This e-mail message and any attachments are confidential. Dissemination, distribution or copying of this e-mail or any attachments by anyone other than the intended recipient is prohibited. If you are not the intended recipient, please notify LifeCell Corporation immediately by replying to this e-mail, and destroy all copies of this e-mail and any attachments. Thank you! *********************************************************************************************************************************************** From gcallis <@t> montana.edu Mon Nov 27 14:45:48 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Nov 27 14:46:08 2006 Subject: [Histonet] Snap freezing undecalcified bone using hexane, OCT ingredients In-Reply-To: <112720061843.19942.456B31E80008867900004DE62160375964050E9 60C9D9A0606@att.net> References: <112720061843.19942.456B31E80008867900004DE62160375964050E960C9D9A0606@att.net> Message-ID: <6.0.0.22.1.20061127134148.01b36518@gemini.msu.montana.edu> At last, an answer! Thank you. But on the other hand, when freezing whole rat femurs, skulls, or where the bone marrow is not exposed and totally surrounded by cortical bone, I guess one could not bother with coating the bones. Wet spongy centers are something I have not experienced when I haven't coated bone but then these were whole samples dissimilar to some of his samples (ostephytes cut off from larger bones). At 11:43 AM 11/27/2006, you wrote: >To answer one of your questions in your e-mail, Dodds told me that he >coats the bone with PVA so that the hexane is not wicked into the bone >marrow. If this happens, you wind up with a wet spongy center. > >Jeff >-------------- Original message from Gayle Callis : >-------------- > > > > We do this all the time, and it can be done two ways. > > > > RA Dodds was one of the first who used this along with van Noorden. Dodds > > published in J of Histotechnology. Check out the following references for > > more on snap freezing bone, particularly the van Noorden paper. Hexane is > > one solvent used and it seems to be a gentler but thorough snap > > freezing. There is extensive discussion about snap freezing bone and the > > various solvents in Histonet archives. > > > > JR Connor, RA Dodds, IE James, and M Gowen > > Human osteoclast and giant cell differentiation: the apparent switch from > > nonspecific esterase to tartrate resistant acid phosphatase activity > > coincides with the in situ expressi! on of osteoponti n mRNA > > J. Histochem. Cytochem., Dec 1995; 43: 1193 - 1201. > > > > RA Dodds, K Merry, A Littlewood, and M Gowen > > Expression of mRNA for IL1 beta, IL6 and TGF beta 1 in developing human > > bone and cartilage > > J. Histochem. Cytochem., Jun 1994; 42: 733 - 744. > > > > CJ Van Noorden, IM Vogels, and RE Smith > > Localization and cytophotometric analysis of cathepsin B activity in > > unfixed and undecalcified cryostat sections of whole rat knee joints > > J. Histochem. Cytochem., May 1989; 37: 617 - 624. > > > > Dodds dipped undecalcified bone in a water soluble 30,000 - 70,000 MW > > polyvinyl alcohol to coat the bone, and then snap froze in a mixture of > dry > > ice and hexane (pieces of dry ice in the hexane itself). We have done this > > but coated the bone with diluted OCT (1:1 OCT 1:1 with water since the OCT > > contains polyvinyl alcohol. Dodds never explained why coating was > > important unless to protect the surface of the bone from some of the > > effects of the solvent. The frozen bone could then be attached to the > > metal disk with either water, OCT, or some other cryoembedding media (2% > > methyl cellulose is a super hard hold for bone). > > > > OCT contains polyvinyl alcohol, polyethylene glycol and water. The > > molecular weights of these chemical are given, a proprietary issue, but we > > use it for undecalcified bone cryotomy along with the Instrumedics > Cryojane > > tape transfer system and have good results. > > > > We prefer to embed the bone in OCT, then snap freeze with the hexane/dry > > ice mixture. Hexane is explosive, and not a good thing to breathe > > in. Fume hoods should be used and some use respirators to protect from > > hexane fumes. > > > > One thing that can happen with liquid nitrogen snap freezing is bone > > shatters or splits. We exper! ienced this with immature bovine bone and > also > > delicate nasal bones on a mouse head which will split apart. The liquid > > nitrogen temperature may be too cold for this calcified matrix but > > hexane/dry ice freezing prevents this shattering. > > > > > > Gayle Callis HTL, HT, MT(ASCP) > > Research Histopathology Supervisor > > Veterinary Molecular Biology > > Montana State University > > Bozeman MT 59717 > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ploykasek <@t> phenopath.com Mon Nov 27 14:49:39 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Mon Nov 27 14:49:55 2006 Subject: [Histonet] automated immuno stainer In-Reply-To: Message-ID: Hi Christine. I believe that a good, well trained tech can achieve good immunos on any type of platform. I think the main issues are your preferences: do you want an open or closed system - any detection system or only the instrument company, concentrated or prediluted antibodies,etc? Antigen retrieval on instrument or ok off instrument? Cost per slide? Ease of waste disposal? Can you use the clones of antibodies you have currently validated on the instrument? User friendly? Those are the main issues I can think of at the moment - my brain is still tired from all the turkey tryptophan! Most companies are more than happy to provide you with a demo & they may even help work up some things for you. As for my preference - it is Dako. We have several of the 48 slide configured Autostainers. I'd be happy to respond to any questions you might have about the Dako. Good luck in your stainer hunting. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Hello all! I hope everyone enjoyed their breif repreive from stress. I was > wondering if anyone could give me some input on automated stainers for > immunohistochemistry. Currently we hand stain and our volumes are steadily > increasing. We are looking at automation as we probably do up to 12-15 stains > per day and plan on starting ER and PR. I heard Vantana is very good. How > about Leica? (Theirs does both immunos and ordianry special stains.) Anyone > have any experience they would like to share? We are an Ascension Health > affiliated hospital as that may matter in the negotiations. Thanks for any and > all opinions. > Respectfullly, > Christine Tambasco, HT (ASCP) > St. Mary's Hospital > Amsterdam, New York 12010 > 518-841-7287 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From zacharyweil <@t> yahoo.com Mon Nov 27 15:43:26 2006 From: zacharyweil <@t> yahoo.com (Zach Weil) Date: Mon Nov 27 15:43:37 2006 Subject: [Histonet] macrophages/microglia in FFPE tissue Message-ID: <993771.375.qm@web50614.mail.yahoo.com> Hello, I am having a very difficult time labelling microglia/macrophages in FFPE tissue. I have tried a couple of OX-42 (Serotec and Chemicon), MHC-II, several lectins and ED-1 (also serotec) and I am consistently getting no positive staining. This tissue works well for other antigens and I have tried a variety of proteolytic antigen retrieval steps to try and get at these antigens. I know that cell surface markers don't survive paraffin embedding to well but I didnt think the lectins or the ED-1 should be that affected by that. If anyone can suggest anything I would really appreciate it. There are only a couple published papers on hamster tissue (not my hamster species) and I've tried their protocols with no success. Thanks, Zach Zachary Weil Departments of Psychology and Neuroscience The Ohio State University --------------------------------- Everyone is raving about the all-new Yahoo! Mail beta. From Heather.Nymeyer <@t> interiorhealth.ca Mon Nov 27 16:34:10 2006 From: Heather.Nymeyer <@t> interiorhealth.ca (Nymeyer, Heather) Date: Mon Nov 27 16:34:20 2006 Subject: [Histonet] automated immuno stainer In-Reply-To: Message-ID: I really do not like to recommend one vendor over another but I must share my experience with you. We were performing manual stains up until three years ago and we purchased an automatic IHC stainer. There was a drastic improvement in the quality of staining and reproducibility. Unfortunately, we did not estimate that our volume of stains would increase as it did and we can not keep up to the number of requests. Although our stainer does produce excellent quality, it is a system that once it starts (regardless if it is full or not) the run can not be interrupted or added to. This has caused us some problems with keeping up with the workload. There are systems that are continuous feed and this allows the user to have three different runs started at separate times. I feel this would be a definite benefit to any lab. Please feel free to contact me if you need any further information. Heather D. Nymeyer, RT, CEBT Charge Technologist, Anatomic Pathology Royal Inland Hospital, Kamloops, BC. 250-314-2664 heather.nymeyer@interiorhealth.ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Monday, November 27, 2006 12:50 PM To: Chistine Tambasco; histonet Subject: Re: [Histonet] automated immuno stainer Hi Christine. I believe that a good, well trained tech can achieve good immunos on any type of platform. I think the main issues are your preferences: do you want an open or closed system - any detection system or only the instrument company, concentrated or prediluted antibodies,etc? Antigen retrieval on instrument or ok off instrument? Cost per slide? Ease of waste disposal? Can you use the clones of antibodies you have currently validated on the instrument? User friendly? Those are the main issues I can think of at the moment - my brain is still tired from all the turkey tryptophan! Most companies are more than happy to provide you with a demo & they may even help work up some things for you. As for my preference - it is Dako. We have several of the 48 slide configured Autostainers. I'd be happy to respond to any questions you might have about the Dako. Good luck in your stainer hunting. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Hello all! I hope everyone enjoyed their breif repreive from stress. I was > wondering if anyone could give me some input on automated stainers for > immunohistochemistry. Currently we hand stain and our volumes are steadily > increasing. We are looking at automation as we probably do up to 12-15 stains > per day and plan on starting ER and PR. I heard Vantana is very good. How > about Leica? (Theirs does both immunos and ordianry special stains.) Anyone > have any experience they would like to share? We are an Ascension Health > affiliated hospital as that may matter in the negotiations. Thanks for any and > all opinions. > Respectfullly, > Christine Tambasco, HT (ASCP) > St. Mary's Hospital > Amsterdam, New York 12010 > 518-841-7287 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ - This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Mon Nov 27 16:49:07 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Nov 27 16:49:23 2006 Subject: [Histonet] endothelial cell specific lectin staining Message-ID: <6.0.0.22.1.20061127154049.01b35e10@gemini.msu.montana.edu> Dear Mark in very snowy Vancouver Canada, There is a lectin specific for endothelial cells. Go to Vector and check out one of their brochure on latest products with info about the tomato lectin. You can perfuse the animal OR use it as a direct conjugate on tissue sections for fluorescent work. Vector may even have an anti tomato lectin antibody available or lectin conjugated to biotin for IHC work. This would eliminate rabbit on rabbit work entirely or you could detect lectin-FITC with an antiFITC. I just ran across a coverphoto of this on BioTechniques, a freebie magazine 39(4)October 2005 and the lectin was applied to rat retinal tissue and it also works in murine tissue. If you can't locate the info on Vector website, I have a citation for this in Am J of Pathology. Good luck Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From gcallis <@t> montana.edu Mon Nov 27 16:59:32 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Nov 27 16:59:53 2006 Subject: [Histonet] Tomato lectin versus UEA1 lectin for endothelial cell question Message-ID: <6.0.0.22.1.20061127155522.01b0a560@gemini.msu.montana.edu> Need to qualify which lectin does what UEA1 can stain vascular endothelial cells and tomato lectin is (as Vector states) "an effective marker of blood vessels and microglial cells in rodents" and is used to examine rodent tumor angiogenesis. Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 From omnivore98 <@t> yahoo.com Mon Nov 27 18:41:14 2006 From: omnivore98 <@t> yahoo.com (Heather Renko) Date: Mon Nov 27 18:41:27 2006 Subject: [Histonet] Richard Allan Message-ID: <49455.65092.qm@web31315.mail.mud.yahoo.com> Hello Fellow Histonetters: I want to hear the good the bad and the ugly on your experience with Richard Allan??? Concerned Customer --------------------------------- Everyone is raving about the all-new Yahoo! Mail beta. From RSRICHMOND <@t> aol.com Mon Nov 27 20:57:28 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Mon Nov 27 20:57:38 2006 Subject: [Histonet] Re: automated immuno stainer Message-ID: I think we had this discussion about automated immunostainers a few days ago. I've been researching this topic for one of my locum tenens clients who wants to get into it. I've found that Ventana has about 70% of the clinical lab market share in the USA. From what I've found out so far, it's probably the better choice for the ordinary medical pathology lab, where overwork is the norm and scientific expertise is usually rather minimal (I'm talking about pathologists when I say that). Bob Richmond Samurai Pathologist Knoxville TN From kellerc2 <@t> uthscsa.edu Mon Nov 27 21:11:58 2006 From: kellerc2 <@t> uthscsa.edu (Keller, Charles) Date: Mon Nov 27 21:12:04 2006 Subject: [Histonet] Re: automated immuno stainer References: Message-ID: I could add that we've liked our Biogenix instrument, but the tech support was unsatisfying and the staff turnover has been worrisome. Charles Keller, MD Assistant Professor, Department of Cellular & Structural Biology Adjunct Assistant Professor, Department of Pediatrics Investigator, Developmental Cancer Genetics and Therapeutics Children's Cancer Research Institute The University of Texas Health Science Center 7703 Floyd Curl Drive, Mail Code 7784 San Antonio, TX 78229-3900 210-562-9062 [office] 210-562-9014 [fax] www.sarcomalab.org kellerc2@uthscsa.edu ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of RSRICHMOND@aol.com Sent: Mon 11/27/2006 8:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: automated immuno stainer I think we had this discussion about automated immunostainers a few days ago. I've been researching this topic for one of my locum tenens clients who wants to get into it. I've found that Ventana has about 70% of the clinical lab market share in the USA. From what I've found out so far, it's probably the better choice for the ordinary medical pathology lab, where overwork is the norm and scientific expertise is usually rather minimal (I'm talking about pathologists when I say that). Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pathrm35 <@t> charter.net Mon Nov 27 21:49:28 2006 From: pathrm35 <@t> charter.net (pathrm35@charter.net) Date: Mon Nov 27 21:49:41 2006 Subject: [Histonet] automated immuno stainer Message-ID: <41660869.1164685768538.JavaMail.root@fepweb09> I've used the Ventana, Biogenex and Vision Biosytem Bond IHC stainers. The Vision and Ventana are more user friendly for online dewaxing and antigen retrieval but I really did like the RTU antibodies from Biogenex.They were very consistent and clean staining. Vision Biosytem (Steve Westra in Florida) did have the best technical support though. At one place I worked, the Dako rep wanted to charge us $1,700.00 to ship a demo IHC stainer for us to try. My MD didn't even consider Dako after that. There is no perfect system. You just need to find the one that will fit your needs and budget. I would suggest getting a solid price quote on the equipment, antibodies and other disposable products prior to purchasing. Ron Martin, BS, HT (ASCP) HTL, QIHC ---- Patti Loykasek wrote: > Hi Christine. I believe that a good, well trained tech can achieve good > immunos on any type of platform. I think the main issues are your > preferences: do you want an open or closed system - any detection system or > only the instrument company, concentrated or prediluted antibodies,etc? > Antigen retrieval on instrument or ok off instrument? Cost per slide? Ease > of waste disposal? Can you use the clones of antibodies you have currently > validated on the instrument? User friendly? Those are the main issues I can > think of at the moment - my brain is still tired from all the turkey > tryptophan! > Most companies are more than happy to provide you with a demo & they may > even help work up some things for you. > As for my preference - it is Dako. We have several of the 48 slide > configured Autostainers. I'd be happy to respond to any questions you might > have about the Dako. > Good luck in your stainer hunting. > > > Patti Loykasek BS, HTL, QIHC > PhenoPath Laboratories > Seattle, WA > > > > > > > > > Hello all! I hope everyone enjoyed their breif repreive from stress. I was > > wondering if anyone could give me some input on automated stainers for > > immunohistochemistry. Currently we hand stain and our volumes are steadily > > increasing. We are looking at automation as we probably do up to 12-15 stains > > per day and plan on starting ER and PR. I heard Vantana is very good. How > > about Leica? (Theirs does both immunos and ordianry special stains.) Anyone > > have any experience they would like to share? We are an Ascension Health > > affiliated hospital as that may matter in the negotiations. Thanks for any and > > all opinions. > > Respectfullly, > > Christine Tambasco, HT (ASCP) > > St. Mary's Hospital > > Amsterdam, New York 12010 > > 518-841-7287 > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------------------------------------------------- > This e-mail message, including any attachments, is for the sole use of > the intended recipients and may contain privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If > you are not the intended recipient, please contact the sender by e-mail > and destroy all copies of the original message, or you may call PhenoPath > Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From adisabag <@t> techunix.technion.ac.il Tue Nov 28 03:11:04 2006 From: adisabag <@t> techunix.technion.ac.il (Adi Sabag) Date: Tue Nov 28 03:11:17 2006 Subject: [Histonet] endothelial cell specific lectin staining- perfusion In-Reply-To: <6.0.0.22.1.20061127154049.01b35e10@gemini.msu.montana.edu> References: <6.0.0.22.1.20061127154049.01b35e10@gemini.msu.montana.edu> Message-ID: <1164705064.456bfd280f703@webmail.technion.ac.il> Dear Gayle, Do you have a good protocol for lactin pefusion? What about Dextran? Thanks- Adi Quoting Gayle Callis : > Dear Mark in very snowy Vancouver Canada, > > There is a lectin specific for endothelial cells. Go to Vector and check > out one of their brochure on latest products with info about the tomato > lectin. You can perfuse the animal OR use it as a direct conjugate on > tissue sections for fluorescent work. Vector may even have an anti > tomato lectin antibody available or lectin conjugated to biotin for IHC > work. This would eliminate rabbit on rabbit work entirely or you could > detect lectin-FITC with an antiFITC. > > I just ran across a coverphoto of this on BioTechniques, a freebie magazine > 39(4)October 2005 and the lectin was applied to rat retinal tissue and it > also works in murine tissue. If you can't locate the info on Vector > website, I have a citation for this in Am J of Pathology. > > Good luck > > > > > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 > 406 994-4303 (FAX) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Adi Sabag Gera Neufeld's laboratory Medicine Faculty, Technion 04-8295425 From rennie11505 <@t> yahoo.com Tue Nov 28 07:38:16 2006 From: rennie11505 <@t> yahoo.com (Adrienne Anderson) Date: Tue Nov 28 07:38:25 2006 Subject: [Histonet] Repost: Question on calcified samples Message-ID: <20061128133816.20602.qmail@web37007.mail.mud.yahoo.com> I wanted to repost this just in case people did not see my message over the holiday break. If anyone else has any advice on this topic, it would be appreciated. Thanks! Hello all, I am a histotechnology student, and my classmates and I will be working with some coral samples this year. I was just wondering if there was a way to prepare frozen sections on calcified samples, and if so, what equipment is needed? Thank you, Adrienne Mazzante Histotechnology Program Medical University of South Carolina --------------------------------- Access over 1 million songs - Yahoo! Music Unlimited. From gcallis <@t> montana.edu Tue Nov 28 09:56:19 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Nov 28 09:56:33 2006 Subject: rabbit on rabbit Re: [Histonet] endothelial cell specific lectin staining In-Reply-To: <012001c7127d$226561f0$a1065486@auxs.umn.edu> References: <6.0.0.22.1.20061127154049.01b35e10@gemini.msu.montana.edu> <012001c7127d$226561f0$a1065486@auxs.umn.edu> Message-ID: <6.0.0.22.1.20061128082851.01b1d6c0@gemini.msu.montana.edu> Mark and Jan, There is an excellent, extensive review of Lectins in J Histochemistry Cytochemistry if you need a place to start on lectin learning. Check out this publication in the same journal, Glycoconjugate expression defines the origin and differentiatian pathway of intestinal M cells. Geber A, Posselt W. 45(10):1341-1350, 1997. This work was done on rabbit tissue. Doing rabbit on rabbit IHC is very much like doing doing mouse on mouse IHC. If one biotinylates the primary and then does the proper normal serum block success may be possible, similar to the mouse on mouse kits using biotinylation. There is a publication from a defunct journal, Cell Vision by Gu J et al, titled a Primary secondary antibody complex method of immunocytochemistry using rabbit polyclonal antibodies to detect antigens in rabbit tissue, 2(1):59, 1995. This journal became Applied Immunohistochemistry and Molecular Morphology later on, and they may be a source of the article since Gu is one of the editors for AIMM. You can also contact me personally if interlibrary loan fails or you can't locate the article. The method is also in a book titled Analytivcal Morphology, Theory, Applications and Protocols, Ed. Jiang Gu, Chapter: Elimination of Background Staining, using the primary secondary antibody complex method. ISBN#1-881299-03-1, soft cover Eaton Publishing 1997 unless out of print. Topics included FISH, immunogold, antigen retrieval, MW, etc). but At 04:38 PM 11/27/2006, you wrote: >Mark, > >Just my two cents worth... from my experience with lectins a couple >decades ago, I found that what worked in one species did not always work >in another... sometimes there was similarity between species, but not >always. So, if you choose to go the lectin route, do a good literature >search first or be prepared to run many trials. > >The cellular glycoconjugates (to which the lectins bind) may not be >exactly the same from species to species. That was my guess at the time, >anyway. > >But, I agree... avoid rabbit antibodies on rabbit tissues, if at all >possible. I've never been able to get decent staining without background >when using unconjugated polyclonal antibodies on rabbit tissues. > >Jan Shivers >Univ. of Minnesota Vet Diag Lab Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 From haldana <@t> unimoron.edu.ar Tue Nov 28 10:03:40 2006 From: haldana <@t> unimoron.edu.ar (Hernan Aldana Marcos) Date: Tue Nov 28 10:02:53 2006 Subject: [Histonet] Re: need a protocol for whole rat brain tissue Message-ID: <033001c71306$c5989070$ec04a8c0@sistemas> Dear Janice Ho I wrote paper dealing with the standardization of Fixation, Processing and Staining Method for whole brains, published in the Journal: Biocell (1996. 20(3): 265-272). I send it to you by mail. Please confirm the reception Hern?n Aldana Dr. Hern?n J. Aldana Marcos Secretario Acad?mico Facultad de Medicina Universidad de Mor?n From bs8asg <@t> bath.ac.uk Sat Nov 18 05:34:35 2006 From: bs8asg <@t> bath.ac.uk (al garfield) Date: Tue Nov 28 10:16:35 2006 Subject: [Histonet] X-gal staining of adult mouse spinal cord Message-ID: <39BACC84F7AA78411FF8F40A@[172.21.6.32]> Dear People, Could anyone provide me with a protocol for the sectioning and x-gal staining of adult spinal cord? I presume cryosections are my best bet....does the tissue require decalcification and if so how is that achieved? Can i get away without fixation? It would ensure i don't compromise B-gal activity. Apologies for the barrage of questions. Any help is appreciated. cheers al garfield ---------------- University of Bath Centre for Regenerative Medicine UK From jkiernan <@t> uwo.ca Tue Nov 21 10:34:53 2006 From: jkiernan <@t> uwo.ca (John A. Kiernan) Date: Tue Nov 28 10:16:38 2006 Subject: [Histonet] marker for brain inflammation References: <3665A19D-43E0-4583-A345-93EE8AE4C30D@bidmc.harvard.edu> <454EBE41.893DE1FA@uwo.ca> Message-ID: <45632AAD.B4515A95@uwo.ca> Checking old emails, I came across this unacknowledged item. Did you receive my reply to your query? If so, was it in any way helpful? John Kiernan Anatomy Dept, U.W.O. London, Canada. ______________________ On 5th November 2006 John Kiernan wrote: > > Inflammation is defined as the response of living > tissue to injury, characterized by vasodilation, > swelling (increased vascular permeability) and > pain. The second of these cardinal signs is > demonstrable by immunostaining for plasma > proteins. In the normal central nervous system > (except in the choroid plexuses, the 5 > circumventricular organs, and at the lamina > cribrosa of the optic nerve head), plasma proteins > occur only within blood vessels. If you see them > outside vessels there has been a failure of the > blood-brain barrier, which could well be due to > inflammation. > > There is an artifact to avoid. Postmortem delay > can allow plasma proteins to diffuse out of the > vasculature and into some neurons and neuroglial > cells. I've seen this in human brain, and the > artifact is well documented also for animals. See: > Mori S, Sternberger N h, Herman NM, Sternberger > l A (1991) Leakage and neuronal uptake of serum > protein in aged and Alzheimer brains: a postmortem > phenomenon with antemortem etiology. Laboratory > Investigation 64: 345-351. > Fabian RH (1992) Poor reliability of > immunocytochemical localization of IgG in > immersion-fixed tissue from the central nervous > system. Journal of Histochemistry and > Cytochemistry 40: 987-991. > Loberg EM, Torvik A (1992) Plasma proteins in > normal neurons: immunohistochemical studies on > autopsy material and experimental animals. Acta > pathologica et microbiologica scandinavica 100: > 431-436. > > A similar artifact is seen if immunohistochemistry > is attempted on unfixed cryostat sections of the > rat's brain. > Sparrow JR (1980) Immunohistochemical study of > the blood-brain barrier. Production of an > artifact. Journal of Histochemistry and > Cytochemistry 26: 570-572. > > Caroline Bass wrote: > > > > Hello, > > > > I'm wondering if someone could suggest a marker or stain that could > > help visualize inflammation. I am working on a project where I > > inject a virus in the brain to introduce genes in neurons. However, > > I want to make sure that the injection itself, or the neuronal > > infection is not causing a large degree of inflammation. Could > > someone suggest a good way to visualize this? I imagine some sort of > > immune marker will do, perhaps mF4/80? > > > > thanks, > > > > Caroline > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louanne.shay.cheever <@t> medtronic.com Tue Nov 21 16:10:49 2006 From: louanne.shay.cheever <@t> medtronic.com (Cheever, Louanne) Date: Tue Nov 28 10:16:42 2006 Subject: [Histonet] Whole brain mounts Message-ID: We are looking for a contract lab who does whole mount large animal brain slides. Routine paraffin, to include H&E staining with possible LFB and GFAP staining. Does any one know a lab who does this? Thanks. Louanne Cheever Histology Supervisor PRL, Medtronic, Inc. 11520 Yellow Pine St. N.W. Minneapolis, MN 55448 763-514-6885 From gcallis <@t> montana.edu Tue Nov 28 10:22:12 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Nov 28 10:22:25 2006 Subject: [Histonet] Repost: Question on calcified samples In-Reply-To: <20061128133816.20602.qmail@web37007.mail.mud.yahoo.com> References: <20061128133816.20602.qmail@web37007.mail.mud.yahoo.com> Message-ID: <6.0.0.22.1.20061128091726.01b6dba8@gemini.msu.montana.edu> Instrumedics Cryojane Tape Transfer, it will work in most cryostats that have a temperature range down to -35C, and D profile tungsten carbide cryostat knives (there require a universal knife holder in the cryostat) Delaware Diamond Knives sell these knives, they cost around $1200 per knife and then ~$250 to recondition. The Cryojane runs ~$7000 or more (the price may have gone up). Coral will be very hard to work with, and doing frozen sections may not be ideal. It is not like bone as pointed out in a previous email. At 06:38 AM 11/28/2006, you wrote: > > I am a histotechnology student, and my classmates and I will be working > with some coral samples this year. I was just wondering if there was a > way to prepare frozen sections on calcified samples, and if so, what > equipment is needed? > > Thank you, > > Adrienne Mazzante > Histotechnology Program > Medical University of South Carolina > > >--------------------------------- >Access over 1 million songs - Yahoo! Music Unlimited. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From olek.michalski <@t> nencki.gov.pl Tue Nov 28 10:26:42 2006 From: olek.michalski <@t> nencki.gov.pl (Olek Michalski) Date: Tue Nov 28 10:24:57 2006 Subject: [Histonet] tumor cryosection In-Reply-To: <1164726307.456c5023304b6@webmail.technion.ac.il> References: <1164615901.456aa0dd87e41@webmail.technion.ac.il> <1164726307.456c5023304b6@webmail.technion.ac.il> Message-ID: Dnia 28-11-2006 o 16:05:07 Adi Sabag napisa?: Indeed, I take the slide out of the cryostat. I don't need the tissue to be frozen after cut. If you do, you could try sucrose solution but I have no idea weather it would help. I use a separate brush to transfer liquid onto section. Just wet the brush and cover the tissue with small volume of buffer. When tissue soaks it detaches from the glass slide, so be aware. I consider it an advantage since I can adjust the section (it sometimes folds or sticks incorrectly). On the other hand I remember the notion about removing air bubbles with dry brush just after collection on the slide. Try searching the list archive (couple of months ago there was an extensive discussion on cryosectioning technique). Last thought: I do not flash freeze my tissue after soaking with sucrose solution. I work with brains, also fixed w/ 4% PFA. I soak tissue with sucrose in two step procedure: first 15% sucrose until the tissue sinks and then in 30% sucrose again until sinks. Then I embed it in OCT and freeze in -20 or on dry ice (large specimens). I think it's posible that your section thaw inequally (ie the part which doesn't come into contact w/ glass keeps more rigid) and that's your problem. Best regards Olek Michalski > Dear Olek, > Thank you for your reply. > I have some questions; > After I pick up the section I keep the slide inside the cryostat. Do you > do that > also or do you leave it outside? Because insidee the cryostat the PBS > will > freeze and than I don't see how it can help... > Do you put the drop of PBS on the tissue or on a region in the slide > without > tissue that is adjacent and then the PBS soaks toward the tissue? > > Thank you! > > Adi Sabag. > > > Quoting Olek Michalski : > >> Dnia 27-11-2006 o 09:25:01 Adi Sabag >> napisa?: >> >> > I'm working with s.c tumors that are approximately 8x8 mm, they were >> > fixed with >> > 4% PFA and then either frozen in isopentene over liquid N2 or I used a >> > protocol >> > of embedding in sucrose and then freezing. In both cases I found that >> the >> > optimal temperature for getting nice sections is around 20C. I'm >> using 3 >> > different sizes of sections- 60, 30 and 10um. The problem is that when >> >> Well, after picking a section I put a drop of PBS onto the slide and let >> the tissue soak. Then it tends to spread on the glass and it's even >> possible to remove an air bubble from underneath with fine brush (while >> working w/ 40um - thinner e. g. 20um tend to tear too much). Of course I >> have to wait till tisue dries to stick to the glass again. >> >> Hope this helps >> Olek Michalski >> > > From kellerc2 <@t> uthscsa.edu Tue Nov 28 10:51:58 2006 From: kellerc2 <@t> uthscsa.edu (Keller, Charles) Date: Tue Nov 28 10:52:12 2006 Subject: [Histonet] X-gal staining of adult mouse spinal cord In-Reply-To: <39BACC84F7AA78411FF8F40A@[172.21.6.32]> Message-ID: The last few pages are the adult x-gal staining method. Hope it works for you. Sincerely, Charles Charles Keller, MD Assistant Professor, Department of Cellular & Structural Biology Adjunct Assistant Professor, Department of Pediatrics Children's Cancer Research Institute The University of Texas Health Science Center 7703 Floyd Curl Drive, Mail Code 7784 San Antonio, TX 78229-3900 210-562-9062 [office] 210-562-9014 [fax] http://ccri.uthscsa.edu or www.sarcomalab.org kellerc2@uthscsa.edu Postdoctoral Opportunities and Undergraduate & Summer Internships: http://ccri.uthscsa.edu/keller CCRI and UTHSCSA wish to promote open communication while protecting confidential and/or privileged information. If you have received this message in error, please inform the sender and delete all copies. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of al garfield Sent: Saturday, November 18, 2006 5:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] X-gal staining of adult mouse spinal cord Dear People, Could anyone provide me with a protocol for the sectioning and x-gal staining of adult spinal cord? I presume cryosections are my best bet....does the tissue require decalcification and if so how is that achieved? Can i get away without fixation? It would ensure i don't compromise B-gal activity. Apologies for the barrage of questions. Any help is appreciated. cheers al garfield ---------------- University of Bath Centre for Regenerative Medicine UK _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Tue Nov 28 11:58:25 2006 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Nov 28 11:57:46 2006 Subject: [Histonet] Richard Allan Message-ID: <6CBA6DC98A079D408C87250591D9DFB802684BFB@bruexchange.digestivespecialists.com> Dear Concerned Customer, I have had nothing but very good experiences with Richard Allan. Service on equipment has been superior. Supplies and equipment has always been delivered when expected. Customer support is excellent. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heather Renko Sent: Monday, November 27, 2006 7:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Richard Allan Hello Fellow Histonetters: I want to hear the good the bad and the ugly on your experience with Richard Allan??? Concerned Customer --------------------------------- Everyone is raving about the all-new Yahoo! Mail beta. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmilne <@t> bccancer.bc.ca Tue Nov 28 12:14:49 2006 From: kmilne <@t> bccancer.bc.ca (Milne, Katy) Date: Tue Nov 28 12:14:57 2006 Subject: [Histonet] Lectins and epithelial cross-reactivity Message-ID: <07979E76B0869D4E8C9FE4AA9FC065780240D180@srvex03.phsabc.ehcnet.ca> Hi all, I was wondering, speaking of lectins, if anyone has experience using a biotinylated lectin in FFPE mouse tumor tissue (not following perfusion, just routine sectioning) to look at angiogenesis within the tumor. I understand that there could be some cross-reactivity to the epithelial cells which for us would be a bit of a problem as our tumors are pretty solid. Has anyone seen the problem and how bad is it? I'm getting tired of trying to get CD31 and CD34 to behave nicely in our FFPE tumors but I need to be able to see the tumor vasculature. Thanks, Katy Milne Deeley Reseach Centre, BC Cancer Agency, Victoria BC From LGaliotto <@t> nch.org Tue Nov 28 12:37:54 2006 From: LGaliotto <@t> nch.org (Galiotto, Laura) Date: Tue Nov 28 12:38:06 2006 Subject: [Histonet] FULL- TIME OPENING FOR HT Message-ID: <270614B321ACB44D8C1D91F4F921FDC304B1618F@NCH01EX02.nch.org> Hello Everyone I have a full-time opening for a Histology Technician, must be ASCP certified. Tues-Sat 3rd shift some flexibility in start time. The hospital is wonderful place to work and excellent benefits. Those that are interested please apply on-line at www.NCH.org Thank you Laura Histology facilitator Northwest Community Hospital Arlington Heights, IL ******************* PLEASE NOTE ******************* This E-Mail/telefax message and any documents accompanying this transmission may contain information that is privileged, confidential, and/or exempt from disclosure under applicable law and is intended solely for the addressee(s) named above. If you are not the intended addressee/recipient, you are hereby notified that any use of, disclosure, copying, distribution, or reliance on the contents of this E-Mail/telefax information is strictly prohibited and may result in legal action against you. Please reply to the sender advising of the error in transmission and immediately delete/destroy the message and any accompanying documents. Thank you. From LGaliotto <@t> nch.org Tue Nov 28 12:42:01 2006 From: LGaliotto <@t> nch.org (Galiotto, Laura) Date: Tue Nov 28 12:42:14 2006 Subject: [Histonet] FW: FULL- TIME OPENING FOR HT Message-ID: <270614B321ACB44D8C1D91F4F921FDC304B16191@NCH01EX02.nch.org> > -----Original Message----- > From: Galiotto, Laura > Sent: Tuesday, November 28, 2006 12:38 PM > To: 'HISTONET@LISTS.UTSOUTHWESTERN.EDU' > Subject: FULL- TIME OPENING FOR HT > > Hello Everyone > I have a full-time opening for a Histology Technician, must be ASCP certified. Tues-Sat 3rd shift some flexibility in start time. The hospital is wonderful place to work and excellent benefits. > Those that are interested please apply on-line at www.NCH.org > > Thank you > Laura > Histology facilitator > Northwest Community Hospital > Arlington Heights, IL > ******************* PLEASE NOTE ******************* This E-Mail/telefax message and any documents accompanying this transmission may contain information that is privileged, confidential, and/or exempt from disclosure under applicable law and is intended solely for the addressee(s) named above. If you are not the intended addressee/recipient, you are hereby notified that any use of, disclosure, copying, distribution, or reliance on the contents of this E-Mail/telefax information is strictly prohibited and may result in legal action against you. Please reply to the sender advising of the error in transmission and immediately delete/destroy the message and any accompanying documents. Thank you. From Jonathan.Arzt <@t> ARS.USDA.GOV Tue Nov 28 13:08:11 2006 From: Jonathan.Arzt <@t> ARS.USDA.GOV (Arzt, Jonathan) Date: Tue Nov 28 13:10:18 2006 Subject: [Histonet] Histotech Fellowship Opportunity Message-ID: <6B4AF69268EFAE4C80A786BA99F7A667B41F84@MD-MAIL-01.ARSNET.ARS.USDA.GOV> HISTOTECHNOLOGIST RESEARCH FELLOWSHIP OPPORTUNITY - HISTOPATHOLOGY ON FOREIGN ANIMAL DISEASES A unique Histotechnologist research fellowship is currently available working within the pathology division of the USDA, Animal Research Service on Plum Island, NY. We have an immediate opening for a 1-2 year fellow with the possibility of extension beyond the initial fellowship contract. The primary mission of the research group is investigation of veterinary diseases of potential threat to US agriculture interests. Currently the greatest emphasis is directed towards characterizing the pathogenesis of foot-and-mouth disease (aphthovirus). The Plum Island Animal Disease Center is located off the East end of Long Island, NY. Transport to and from the island is provided free to employees aboard government-operated ferries servicing Orient, NY and Old Saybrook, CT. Rural and suburban communities are abundant near both the NY and CT sides of the ferries with access to scenic beaches and vineyards. New York City and Boston are both within 3-4hrs drive. Activities will be varied but will include conventional histotech work, cryosectioning, immunohistochemistry, in-situ hybridization, confocal microscopy, and assistance with animal necropsies and sample collection. The pathology group currently consists of two veterinary pathologists and one other histotech; the group works closely with on-site molecular biologists, immunologists, clinicians, and research fellows. Stipends (ranging from $35,000 to $45,000 a year) will be determined according to education and experience in the field. This fellowship is open to applicants with at least a bachelor's degree and experience in histotechnology. General aspects of the fellowship are described and application is available at: www.orau.gov/piadc For more information, email to the address below. If applying, please notify J. Arzt directly in addition to filing the application with ORISE/ORAU. ******************************************************** Jonathan Arzt DVM, MPVM, DACVP Foreign Animal Disease Research Unit USDA/ARS Plum Island Animal Disease Center P.O. Box 848, Greenport NY 11944 631-323-3171(ph) 631-323-3006(fax) Jonathan.Arzt@ARS.USDA.GOV ******************************************************** From ploykasek <@t> phenopath.com Tue Nov 28 13:21:33 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Tue Nov 28 13:21:51 2006 Subject: [Histonet] Job opening at PhenoPath Laboratories Message-ID: Hi all. I would like to announce a job opening at PhenoPath Laboratories in Seattle, WA. The opening is in the clinical IHC division for a part-time technologist (0.75 FTE), day shift. Responsibilities may include performing IHC, IF, molecular testing on patient samples and tissue sectioning (paraffin and frozen). Participation in the development of new tests or technologies is included. Quality control, safety, and preventive maintenance procedures and documentation are required elements of this position as well. Strong preference given to ASCP certified (or eligible) laboratory techs. PhenoPath Laboratories is a national specialty pathology laboratory committed to providing outstanding clinical and research services. We offer an excellent compensation and benefits package, including 401K. Salary will be determined based on the overall skills and background of the chosen applicant. To apply, please e-mail or fax resume & cover letter to: PhenoPath Laboratories 551 North 34th Street, Suite 100 Seattle, WA 98103 Fax: 206.374.9009 Email: ihctech@phenopath.com Web: www.phenopath.com Employment applications available at www.phenopath.com ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From mail2melody <@t> yahoo.com Tue Nov 28 13:27:46 2006 From: mail2melody <@t> yahoo.com (Melody Thiessen) Date: Tue Nov 28 13:27:55 2006 Subject: [Histonet] flourescent microscope Message-ID: <20061128192746.23061.qmail@web36604.mail.mud.yahoo.com> Hi there Histonetters! It's been a while, but I have a friend who is in the market for a flourescent microscope; and needs to start pricing them for an equipment grant. She's looking for an upright (light source from the top) possibly with a monitor/video feed as well as the standard eyepieces. She needs UV, red, and blue filters as well. Any leads are appreciated. Thanks! Melody Thiessen ____________________________________________________________________________________ Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail beta. http://new.mail.yahoo.com From MElliott <@t> mrl.ubc.ca Tue Nov 28 13:36:51 2006 From: MElliott <@t> mrl.ubc.ca (Mark Elliott) Date: Tue Nov 28 13:38:23 2006 Subject: [Histonet] Cytochrome P450 antibody staining Message-ID: <456C1F53020000D60002C069@mail.mrl.ubc.ca> Hi Does anyone have any experience staining for cytochrome p450 in paraffin embedded tissues? We are looking at CYP1A1 specifically. We can't seem to get it to stain and are looking for any hints/ideas. We are using human lung tissue. Thanks Mark ***CONFIDENTIALITY NOTICE*** This electronic message is intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. From conniegrubaugh <@t> hotmail.com Tue Nov 28 14:47:34 2006 From: conniegrubaugh <@t> hotmail.com (connie grubaugh) Date: Tue Nov 28 14:47:47 2006 Subject: [Histonet] Richard Allan In-Reply-To: <49455.65092.qm@web31315.mail.mud.yahoo.com> Message-ID: I have worked at a few different labs in California and Nevada and every experience with Richard Allen has always been good. Connie Grubaugh Las Vegas Nv. >From: Heather Renko >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Richard Allan Date: Mon, 27 Nov 2006 16:41:14 -0800 >(PST) > >Hello Fellow Histonetters: > > I want to hear the good the bad and the ugly on your experience with >Richard Allan??? > > Concerned Customer > > >--------------------------------- >Everyone is raving about the all-new Yahoo! Mail beta. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ MSN Shopping has everything on your holiday list. Get expert picks by style, age, and price. Try it! http://shopping.msn.com/content/shp/?ctId=8000,ptnrid=176,ptnrdata=200601&tcode=wlmtagline From carl.hobbs <@t> kcl.ac.uk Tue Nov 28 15:01:16 2006 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Tue Nov 28 15:01:32 2006 Subject: [Histonet] re: macrophages/microglia in FFPE tissue Message-ID: <000501c71330$589583a0$0201a8c0@carlba65530bda> Hi Zach. Perhaps you need to try heat-induced epitope retrieval/unmasking on you pwax sections? I have tried an OX-42 Ab reagent on FFPW sections after the above with negative results. ( and tried various proteolytic enzmes). The only excellent result , for me, was after HIER on pwax rat brain, using an anti-phosphotyrosine Ab reagent. See here for your evaluation; http://www.immunoportal.com/index.php Go to Image gallery and use search box "phosphotyrosine". No guarantees for your species, of course. Good luck. carl From pruegg <@t> ihctech.net Tue Nov 28 15:41:08 2006 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Nov 28 15:41:26 2006 Subject: [Histonet] automated immuno stainer In-Reply-To: <41660869.1164685768538.JavaMail.root@fepweb09> Message-ID: <004001c71335$eaf38210$6501a8c0@Patsy> I agree with Patti. I have used Ventana, Biogenex and Dako IHC stainers in research and clinical settings. For cost effectiveness, consistent, reliable results I choose the Dako. I have had the same Dako rep for over 10 years and she is great (LuAnne you know who I am talking about). I did not have to pay for the shipping of my instrument and use the instrument based on reagent requisition. We worked together for weeks validating the antibodies I use and they even provided the reagents in some cases. I know Dako has had some troubles of late, but for my money and satisfaction they have and continue to serve me well. Patsy Ruegg -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pathrm35@charter.net Sent: Monday, November 27, 2006 8:49 PM To: histonet; Patti Loykasek; Chistine Tambasco Subject: Re: [Histonet] automated immuno stainer I've used the Ventana, Biogenex and Vision Biosytem Bond IHC stainers. The Vision and Ventana are more user friendly for online dewaxing and antigen retrieval but I really did like the RTU antibodies from Biogenex.They were very consistent and clean staining. Vision Biosytem (Steve Westra in Florida) did have the best technical support though. At one place I worked, the Dako rep wanted to charge us $1,700.00 to ship a demo IHC stainer for us to try. My MD didn't even consider Dako after that. There is no perfect system. You just need to find the one that will fit your needs and budget. I would suggest getting a solid price quote on the equipment, antibodies and other disposable products prior to purchasing. Ron Martin, BS, HT (ASCP) HTL, QIHC ---- Patti Loykasek wrote: > Hi Christine. I believe that a good, well trained tech can achieve good > immunos on any type of platform. I think the main issues are your > preferences: do you want an open or closed system - any detection system or > only the instrument company, concentrated or prediluted antibodies,etc? > Antigen retrieval on instrument or ok off instrument? Cost per slide? Ease > of waste disposal? Can you use the clones of antibodies you have currently > validated on the instrument? User friendly? Those are the main issues I can > think of at the moment - my brain is still tired from all the turkey > tryptophan! > Most companies are more than happy to provide you with a demo & they may > even help work up some things for you. > As for my preference - it is Dako. We have several of the 48 slide > configured Autostainers. I'd be happy to respond to any questions you might > have about the Dako. > Good luck in your stainer hunting. > > > Patti Loykasek BS, HTL, QIHC > PhenoPath Laboratories > Seattle, WA > > > > > > > > > Hello all! I hope everyone enjoyed their breif repreive from stress. I was > > wondering if anyone could give me some input on automated stainers for > > immunohistochemistry. Currently we hand stain and our volumes are steadily > > increasing. We are looking at automation as we probably do up to 12-15 stains > > per day and plan on starting ER and PR. I heard Vantana is very good. How > > about Leica? (Theirs does both immunos and ordianry special stains.) Anyone > > have any experience they would like to share? We are an Ascension Health > > affiliated hospital as that may matter in the negotiations. Thanks for any and > > all opinions. > > Respectfullly, > > Christine Tambasco, HT (ASCP) > > St. Mary's Hospital > > Amsterdam, New York 12010 > > 518-841-7287 > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------------------------------------------------- > This e-mail message, including any attachments, is for the sole use of > the intended recipients and may contain privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If > you are not the intended recipient, please contact the sender by e-mail > and destroy all copies of the original message, or you may call PhenoPath > Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Tue Nov 28 15:48:25 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Nov 28 15:48:46 2006 Subject: [Histonet] automated immuno stainer Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203708566@sjhaexc02.sjha.org> I second! - even with the problems of late, I have always had good service from DAKO - all the way back to the early 80s, when I was very green behind the ears and they walked me through the manual stains holding my hands. They are a very good company. Joyce Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From JPR <@t> Stowers-Institute.org Tue Nov 28 18:23:53 2006 From: JPR <@t> Stowers-Institute.org (Rey, Jean-Philippe) Date: Tue Nov 28 18:24:24 2006 Subject: [Histonet] Missouri Society for Histotechnologists rewards Message-ID: Hello everyone, Does someone know one of these persons? -Bill DeSalvo -Chris Clark Casey -Jerry Fredenbergh -Elvie Taylor -Billie Carney -Ellen Ellis I try to contact them concerning an award their received from the Missouri Society for Histotechnologists between 1991 and 2006. Thanks for you help!! Jean-Philippe Rey Histology Specialist II Histology Core Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 (816) 926-4305 From tinayates <@t> comcast.net Tue Nov 28 18:42:57 2006 From: tinayates <@t> comcast.net (Comcast Mail) Date: Tue Nov 28 18:43:13 2006 Subject: [Histonet] SEEKING PAUL HASSEMER Message-ID: <02af01c7134f$52f04290$71f9c147@DFC4J821> Paul Hassemer, please contact me at your convenience. Thanks. Tina Yates, HT(ASCP) Sr. Lab Specialist Salem Hospital Regional Laboratory P: 503.561.5443 F: 503.561.4781 tina.yates@salemhospital.org From djmr55 <@t> hotmail.com Tue Nov 28 19:11:36 2006 From: djmr55 <@t> hotmail.com (donna rossi) Date: Tue Nov 28 19:11:46 2006 Subject: [Histonet] Room temperature Message-ID: Hi Everyone in Histoland! Can someone please tell me what is the correct room temperature range for a histology lab that has an autoimmunostainer located within it. Also, at what temperature should paraffin blocks be stored at? Thanks, Donna ,SRHS [emwink.gif] donnarossi [emcocktl.gif] _________________________________________________________________ [1]Stay up-to-date with your friends through the Windows Live Spaces friends list. References 1. http://g.msn.com/8HMBENUS/2737??PS=47575 From omnivore98 <@t> yahoo.com Tue Nov 28 19:31:15 2006 From: omnivore98 <@t> yahoo.com (Heather Renko) Date: Tue Nov 28 19:31:25 2006 Subject: [Histonet] Thank you all for your feedback Message-ID: <20061129013115.76267.qmail@web31310.mail.mud.yahoo.com> To all, thank you for your positive feedback regarding RA. It really helped me with my perspective on service for capital equipment. Heather --------------------------------- Access over 1 million songs - Yahoo! Music Unlimited. From stevenk <@t> med.usyd.edu.au Tue Nov 28 22:39:51 2006 From: stevenk <@t> med.usyd.edu.au (stevenk) Date: Tue Nov 28 22:38:53 2006 Subject: [Histonet] weils stain for myelin Message-ID: I am asking for a colleague who was doing a Weil's stain for myelin. One of the ingredients, 2% alcoholic haematoxylin requires a ripening process for at least 6 months. As the student cannot wait that long, I was wondering if anyone could supply a quicker method, or suggest something to hasten the ripening process of the above. Thanks from down under Stephen -- |Stephen Kum Jew |Senior Technical Officer |Discipline of Pathology |School of Medical Sciences |Blackburn Building D06 |University of Sydney NSW 2006 |Australia |Ph: + 61 2 9351 6143 |Fax:+61 2 9351 3429 From llewllew <@t> shaw.ca Tue Nov 28 23:54:35 2006 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Tue Nov 28 23:54:53 2006 Subject: [Histonet] weils stain for myelin References: Message-ID: <000d01c7137a$d936bed0$130e4246@yourlk4rlmsu> Make up a fresh solution and just dust a little sodium iodate into it, but not too much. Leave half an hour and use. Alternatively, use a fresh solution and leave it ten minutes after adding the mordant. It will ripen OK. The method is done by inspection anyway, so just keep an eye on the staining and adjust the times. Bryan Llewellyn ----- Original Message ----- From: "stevenk" To: Sent: Tuesday, November 28, 2006 8:39 PM Subject: [Histonet] weils stain for myelin > > I am asking for a colleague who was doing a Weil's stain for myelin. > > One of the ingredients, 2% alcoholic haematoxylin requires a ripening > process for at least 6 months. > > As the student cannot wait that long, I was wondering if anyone could > supply a quicker method, or suggest something to hasten the ripening > process of the above. > > Thanks from down under > > Stephen > -- > |Stephen Kum Jew > |Senior Technical Officer > |Discipline of Pathology > |School of Medical Sciences > |Blackburn Building D06 > |University of Sydney NSW 2006 > |Australia > |Ph: + 61 2 9351 6143 > |Fax:+61 2 9351 3429 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Megan.Clarke <@t> hnehealth.nsw.gov.au Wed Nov 29 01:23:31 2006 From: Megan.Clarke <@t> hnehealth.nsw.gov.au (Megan Clarke) Date: Wed Nov 29 01:24:08 2006 Subject: [Histonet] Orcein stain for fat in paraffin sections Message-ID: Hi All Hope that someone out there has this old stain procedure, an Orcein stain to stain for fat in paraffin sections. We had tissue erronously placed in formalin and subsequently processed. The pathologist would like to demonstrate fat using the orcein method. Thanks Zenobia Haffajee HAPS, IHC, Anatomical pathology Newcastle Australia. From adisabag <@t> techunix.technion.ac.il Wed Nov 29 04:40:06 2006 From: adisabag <@t> techunix.technion.ac.il (Adi Sabag) Date: Wed Nov 29 04:40:15 2006 Subject: [Histonet] Anti GFP on cryosections Message-ID: <1164796806.456d6386dedc5@webmail.technion.ac.il> Hi All, Does anyone have an experience with anti-GFP antibody of MBL on cryosections? Thanks- Adi -- Adi Sabag Tumor Progression and Angiogenesis laboratory Medicine Faculty, Technion Haifa, Israel. 972-4-8295425 From brunella.spaggiari <@t> nemo.unipr.it Wed Nov 29 05:20:59 2006 From: brunella.spaggiari <@t> nemo.unipr.it (Brunella Spaggiari) Date: Wed Nov 29 05:23:11 2006 Subject: [Histonet] Von Kossa stain Message-ID: <003301c713a8$72cd5040$ba3a4ea0@PC186Gabbi> We work on undelcalcified bone tissue (human, rabbit) fixed in PFA 4% and embedded in methylmethacrilate (MMA). >From our samples we obtain 50 ?m thick sections which can contain titanium implants. We tried to perform Von Kossa stain (see attachment), always on undeplasticized sections, but we met several problems: - calcium deposits after silver nitrate+UV light did not appear black but brown, and, after sodium thiosulphate, brown stain turns more and more light; - we could not find a suitable counterstain for osteoid: nuclear fast red and neutral red do not stain at all, basic or acid fuchsin stain too much and cover the underlying brown.. Some suggestions? Are there other good histological stainings for undeplasticized bone sections in your experience? Thank you! Brunella From Dorothy.L.Webb <@t> HealthPartners.Com Wed Nov 29 06:19:36 2006 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Wed Nov 29 06:19:46 2006 Subject: [Histonet] FW: controls Message-ID: <0E394B648E5284478A6CCB78E5AFDA27036407AC@hpes1.HealthPartners.int> Trying this message again, as we still need to come up with a good control for ALK-1!! > -----Original Message----- > From: Webb, Dorothy L > Sent: Tuesday, November 14, 2006 10:52 AM > To: 'Histonet@lists.utsouthwestern.edu' > Subject: controls > > Does anyone know of a possible source to purchase ALK-1 controls? We > cannot seem to come up with a good tissue control and have used > CellMarque as our source, but, they are not available through them at > this time. Appreciate any help in this area and thanks ahead of > time!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From coleman_manufacturing <@t> yahoo.com Wed Nov 29 07:31:20 2006 From: coleman_manufacturing <@t> yahoo.com (Coleman Manufacturing) Date: Wed Nov 29 07:31:27 2006 Subject: [Histonet] Fwd: Fwd: FW: READ!!!!!!! Message-ID: <20061129133120.96227.qmail@web37606.mail.mud.yahoo.com> Date: Tue, 28 Nov 2006 23:17:46 -0500 From: "Sara L. Browder" To: coleman_manufacturing@yahoo.com Subject: Fwd: Fwd: FW: READ!!!!!!! ----- Forwarded message from steelea3@mailbox.sc.edu ----- Date: Tue, 28 Nov 2006 20:31:49 -0500 From: steelea3@mailbox.sc.edu Reply-To: steelea3@mailbox.sc.edu Subject: Fwd: FW: READ!!!!!!! To: browdesl@mailbox.sc.edu ----- Forwarded message from kkyle lavette ----- Date: Wed, 29 Nov 2006 00:56:44 +0000 From: kkyle lavette Reply-To: kkyle lavette Subject: FW: READ!!!!!!! To: bucablack2@aol.com, MusicalSMJ@aol.com, robtbarton@aol.com, DonnaB210@aol.com, braziel@webmail.sc.edu, xxraiderxx72@hotmail.com, conleydarrick@yahoo.com, daiglep@mailbox.sc.edu, DAVISKE@gwm.sc.edu, johnson@cadetmail.uscga.edu, canesnj8@aol.com, ashkirk09@yahoo.com, G_Knight_21@hotmail.com, jimv@cox.net, jpvasellina@hotmail.com, jvas@bellsouth.net, STEELEA3@mailbox.sc.edu, napole5127@aol.com, rose.shores@pw.utc.com, kels1021@hotmail.com, mike787b@hotmail.com, johnnyv@ntplx.net, Xaviar3@aol.com, mckier@mailbox.sc.edu, idodough@hotmail.com, dlavette@ofalaw.com >From: HANDKL@mailbox.sc.edu >To: HANDKL@mailbox.sc.edu >Subject: READ!!!!!!! >Date: Mon, 27 Nov 2006 22:12:26 -0500 (EST) > >Read carefully... > > >THIS TOOK TWO PAGES OF THE TUESDAY USA TODAY - IT IS FOR REAL > >To all of my friends, I do not usually forward messages, >But this is from my friend Pearlas Sandborn and she really is >an attorney. > >If she says that this will work - It will work. After all,What have >you got to lose? > >SORRY EVERYBODY.. JUST HAD TO TAKE THE CHANCE!!! I'm an >attorney, And I know the law. This thing is for real. Rest assured >AOL and &nbs p; Intel will follow through with their promises for >fear of facing a multimillion-dollar class action suit similar to the one >filed by PepsiCo against General Electric not too long ago. > >Dear Friends: Please do not take this for a junk letter. >Bill Gates sharing his fortune. If you ignore this, You will repent >later. > >Microsoft and AOL are now the largest Internet companies >and in an effort to make sure that Internet Explorer remains the >most widely used program, Microsoft and AOL are running an e-mail >beta test. > >When you forward this e-mail to friends, Microsoft can and will >track it (If you are a Microsoft Windows user) For a two weeks >time period. > >For every person that you forward this e-mail to, Microsoft will pay >you $245.00 For every person that you sent it to that forwards it on, >Microsoft will pay you $243.00 and for every third person that receives >it, You will be paid $241.00. Within two weeks, Microsoft will contact >you for your address and then send you a check. > >Regards. Charles S Bailey General Manager Field Operations >1-800-842-2332 Ext. 1085 or 904-1085 or RNX 292-1085 > > >Thought this was a scam myself, But two weeks after receiving this >e-mail and forwarding it on. Microsoft contacted me for my address and >within days, I received a check for $24, 800.00. You need to respond >before the beta testing is over. If anyone can affoard this, Bill gates is >the >man. > >It's all marketing expense to him. Please forward this to as many >people as possible. You are bound to get at least $10, 000.00 >We're not going to help them out with their e-mail beta test without >getting a little something for our time. My brother's girlfriend got in >on this a few months ago. When I went to visit him for the Baylor/UT >game, she showed me her check. It was for the sum of $4, 324.44 and >was stamped "Paid In Full". > > > > > > > > > > >_____________________ Confidentiality _____________________ > > > >This electronic transmission is strictly confidential and intended > >solely for the addressee. It may contain information which is covered > >by legal, professional or other privilege. If you are not the intended > >addressee, you must not disclose, copy or take any action in reliance > >of this transmission. If you have received this transmission in error, > >please notify us and delete the received data as soon as possible. > > > >This footnote also confirms that this email message has been swept > >for the presence of computer viruses. > >_______________________________________________________ > > > > > > > > Adalgiza "ADA" Storto > >A & D SupportWear Inc. > >5919 Greenville Ave. #380 > > Dallas,TX 75206 > > 214-883-7373 > > office: 214-363-1600 / 972- 414- 3701 > > fax: 214-265-7655 / 972- 414-3718 > >e-mail: adarx2000@yahoo.com > > > > > > > > > >------------------------------------------------------------------------------ - - > > > > > >IMPORTANT: The sender intends that this electronic message is for >the exclusive use of the person to whom it is addressed. This >message may contain information that is confidential. If the reader >of this message is not an intended recipient, be aware that any >disclosure, dissemination, distribution or copying of this >communication, or the use of its contents, is prohibited. If you >have received this message in error, then please immediately notify >the sender of your inadvertent receipt and delete this message from >all data storage systems. Thank you. _________________________________________________________________ Fixing up the home? Live Search can help http://imagine-windowslive.com/search/kits/default.aspx?kit=improve&locale=en- US&source=hmemailtaglinenov06&FORM=WLMTAG ----- End forwarded message ----- ----- End forwarded message ----- Sara L. Browder From LRaff <@t> lab.uropartners.com Wed Nov 29 07:58:33 2006 From: LRaff <@t> lab.uropartners.com (Lester Raff) Date: Wed Nov 29 07:58:43 2006 Subject: [Histonet] Don't fall for the Hoax in a just posted histonet Message-ID: <5DA1CA5D0B98A84985B545A24423B822019ADA@UPLAB01.uplab.local> See below before you expect any of Bill Gates' money. http://www.hoax-slayer.com/ms-money-giveway-hoax.html Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 From GDawson <@t> dynacaremilwaukee.com Wed Nov 29 08:17:36 2006 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Wed Nov 29 08:23:29 2006 Subject: [Histonet] Don't fall for the Hoax in a just posted histonet Message-ID: It never ceases to amaze that people still fall for this garbage. WOW. I just wish they would come up with something more original like an upcoming diamond meteor shower or a new laser that turns used kleenex into gold. Gulliblely Yours, Glen Dawson Milwaukee, WI From anne.lewin <@t> bms.com Wed Nov 29 08:34:43 2006 From: anne.lewin <@t> bms.com (Anne C Lewin) Date: Wed Nov 29 08:35:54 2006 Subject: [Histonet] Fwd: Fwd: FW: READ!!!!!!! In-Reply-To: <20061129133120.96227.qmail@web37606.mail.mud.yahoo.com> References: <20061129133120.96227.qmail@web37606.mail.mud.yahoo.com> Message-ID: <456D9A83.1040207@bms.com> *This is a hoax*, started in 1997 for more info, visit Snopes.com, they are great when it comes to de-mything urban legends...... http://www.snopes.com/inboxer/nothing/microsoft-aol.asp Coleman Manufacturing wrote: > Date: Tue, 28 Nov 2006 23:17:46 -0500 >From: "Sara L. Browder" >To: coleman_manufacturing@yahoo.com >Subject: Fwd: Fwd: FW: READ!!!!!!! > > > >----- Forwarded message from steelea3@mailbox.sc.edu ----- >Date: Tue, 28 Nov 2006 20:31:49 -0500 >From: steelea3@mailbox.sc.edu >Reply-To: steelea3@mailbox.sc.edu >Subject: Fwd: FW: READ!!!!!!! >To: browdesl@mailbox.sc.edu > > > >----- Forwarded message from kkyle lavette ----- >Date: Wed, 29 Nov 2006 00:56:44 +0000 >From: kkyle lavette >Reply-To: kkyle lavette >Subject: FW: READ!!!!!!! >To: bucablack2@aol.com, MusicalSMJ@aol.com, robtbarton@aol.com, >DonnaB210@aol.com, braziel@webmail.sc.edu, xxraiderxx72@hotmail.com, >conleydarrick@yahoo.com, daiglep@mailbox.sc.edu, DAVISKE@gwm.sc.edu, >johnson@cadetmail.uscga.edu, canesnj8@aol.com, ashkirk09@yahoo.com, >G_Knight_21@hotmail.com, jimv@cox.net, jpvasellina@hotmail.com, >jvas@bellsouth.net, STEELEA3@mailbox.sc.edu, napole5127@aol.com, >rose.shores@pw.utc.com, kels1021@hotmail.com, mike787b@hotmail.com, >johnnyv@ntplx.net, Xaviar3@aol.com, mckier@mailbox.sc.edu, >idodough@hotmail.com, dlavette@ofalaw.com > > > > > > >>From: HANDKL@mailbox.sc.edu >>To: HANDKL@mailbox.sc.edu >>Subject: READ!!!!!!! >>Date: Mon, 27 Nov 2006 22:12:26 -0500 (EST) >> >>Read carefully... >> >> >>THIS TOOK TWO PAGES OF THE TUESDAY USA TODAY - IT IS FOR REAL >> >>To all of my friends, I do not usually forward messages, >>But this is from my friend Pearlas Sandborn and she really is >>an attorney. >> >>If she says that this will work - It will work. After all,What have >>you got to lose? >> >>SORRY EVERYBODY.. JUST HAD TO TAKE THE CHANCE!!! I'm an >>attorney, And I know the law. This thing is for real. Rest assured >>AOL and &nbs p; Intel will follow through with their promises for >>fear of facing a multimillion-dollar class action suit similar to the one >>filed by PepsiCo against General Electric not too long ago. >> >>Dear Friends: Please do not take this for a junk letter. >>Bill Gates sharing his fortune. If you ignore this, You will repent >>later. >> >>Microsoft and AOL are now the largest Internet companies >>and in an effort to make sure that Internet Explorer remains the >>most widely used program, Microsoft and AOL are running an e-mail >>beta test. >> >>When you forward this e-mail to friends, Microsoft can and will >>track it (If you are a Microsoft Windows user) For a two weeks >>time period. >> >>For every person that you forward this e-mail to, Microsoft will pay >>you $245.00 For every person that you sent it to that forwards it on, >>Microsoft will pay you $243.00 and for every third person that receives >>it, You will be paid $241.00. Within two weeks, Microsoft will contact >>you for your address and then send you a check. >> >>Regards. Charles S Bailey General Manager Field Operations >>1-800-842-2332 Ext. 1085 or 904-1085 or RNX 292-1085 >> >> >>Thought this was a scam myself, But two weeks after receiving this >>e-mail and forwarding it on. Microsoft contacted me for my address and >>within days, I received a check for $24, 800.00. You need to respond >>before the beta testing is over. If anyone can affoard this, Bill gates is >>the >>man. >> >>It's all marketing expense to him. Please forward this to as many >>people as possible. You are bound to get at least $10, 000.00 >>We're not going to help them out with their e-mail beta test without >>getting a little something for our time. My brother's girlfriend got in >>on this a few months ago. When I went to visit him for the Baylor/UT >>game, she showed me her check. It was for the sum of $4, 324.44 and >>was stamped "Paid In Full". >> >> >> >> >> >> >> >> >> >> >>_____________________ Confidentiality _____________________ >> >> >> >>This electronic transmission is strictly confidential and intended >> >>solely for the addressee. It may contain information which is covered >> >>by legal, professional or other privilege. If you are not the intended >> >>addressee, you must not disclose, copy or take any action in reliance >> >>of this transmission. If you have received this transmission in error, >> >>please notify us and delete the received data as soon as possible. >> >> >> >>This footnote also confirms that this email message has been swept >> >>for the presence of computer viruses. >> >>_______________________________________________________ >> >> >> >> >> >> >> >>Adalgiza "ADA" Storto >> >>A & D SupportWear Inc. >> >>5919 Greenville Ave. #380 >> >>Dallas,TX 75206 >> >>214-883-7373 >> >>office: 214-363-1600 / 972- 414- 3701 >> >>fax: 214-265-7655 / 972- 414-3718 >> >>e-mail: adarx2000@yahoo.com >> >> >> >> >> >> >> >> >> >>------------------------------------------------------------------------------ >> >> >- >- > > >> >> >> >>IMPORTANT: The sender intends that this electronic message is for >>the exclusive use of the person to whom it is addressed. This >>message may contain information that is confidential. If the reader >>of this message is not an intended recipient, be aware that any >>disclosure, dissemination, distribution or copying of this >>communication, or the use of its contents, is prohibited. If you >>have received this message in error, then please immediately notify >>the sender of your inadvertent receipt and delete this message from >>all data storage systems. Thank you. >> >> > >_________________________________________________________________ >Fixing up the home? Live Search can help >http://imagine-windowslive.com/search/kits/default.aspx?kit=improve&locale=en- >US&source=hmemailtaglinenov06&FORM=WLMTAG > > >----- End forwarded message ----- > > > >----- End forwarded message ----- > > >Sara L. Browder > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From petepath <@t> yahoo.com Wed Nov 29 09:02:35 2006 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Wed Nov 29 09:02:46 2006 Subject: [Histonet] PA opening Message-ID: <324544.3281.qm@web30413.mail.mud.yahoo.com> Looking for a Pathologist's Assistant at Hackensack University Medical Center in lovely Bergen County New Jersey. A great experience in one of New Jersey's finest hospitals. Interested candidates please contact Jackie Brown Histology lab supervisor at : jbrown@humed.com. Stephen Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com From Nicholas_Gidmark <@t> brown.edu Wed Nov 29 09:07:07 2006 From: Nicholas_Gidmark <@t> brown.edu (Gidmark, Nicholas) Date: Wed Nov 29 09:07:17 2006 Subject: [Histonet] weils stain for myelin References: Message-ID: <6179894FC62F50438F1862775766F86904E25841@MAIL2.AD.Brown.Edu> I've used Erlich's haematoxylin and my recipe has 0.5 grams of sodium iodate as a good instant-ripener for 600ml of solution, just to give you an idea of scale. Good luck! --Nick Nicholas J. Gidmark, graduate student Brainerd lab of biomechanics and functional morphology Dept. of Ecology & Evolutionary Biology Brown University 80 Waterman Street, box G-W Providence, RI 02912 nicholas_gidmark@brown.edu Cell: 612.386.0042 Lab: 401.863.1032 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of stevenk Sent: Tue 28-Nov-06 11:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] weils stain for myelin I am asking for a colleague who was doing a Weil's stain for myelin. One of the ingredients, 2% alcoholic haematoxylin requires a ripening process for at least 6 months. As the student cannot wait that long, I was wondering if anyone could supply a quicker method, or suggest something to hasten the ripening process of the above. Thanks from down under Stephen -- |Stephen Kum Jew |Senior Technical Officer |Discipline of Pathology |School of Medical Sciences |Blackburn Building D06 |University of Sydney NSW 2006 |Australia |Ph: + 61 2 9351 6143 |Fax:+61 2 9351 3429 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Nov 29 09:10:40 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Nov 29 09:10:48 2006 Subject: [Histonet] Room temperature In-Reply-To: Message-ID: <20061129151040.66502.qmail@web61216.mail.yahoo.com> Donna: We always had 75 ?F at our lab, and that was also the temperature of our blocks room (were we kept 9 years worth of regular blocks + all the "saves for ever"). If the temperature at your lab is maintained constant, it does not matter much which, your IHC procedures will be run at such a temp. and your protocols will be "self corrected" to it, meaning that when you select a dilution rate, the temperature effect will be "implicitly" included. Ren? J. donna rossi wrote: Hi Everyone in Histoland! Can someone please tell me what is the correct room temperature range for a histology lab that has an autoimmunostainer located within it. Also, at what temperature should paraffin blocks be stored at? Thanks, Donna ,SRHS [emwink.gif] donnarossi [emcocktl.gif] _________________________________________________________________ [1]Stay up-to-date with your friends through the Windows Live Spaces friends list. References 1. http://g.msn.com/8HMBENUS/2737??PS=47575 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Want to start your own business? Learn how on Yahoo! Small Business. From rjbuesa <@t> yahoo.com Wed Nov 29 09:15:44 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Nov 29 09:15:53 2006 Subject: [Histonet] FW: controls In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA27036407AC@hpes1.HealthPartners.int> Message-ID: <258248.10061.qm@web61218.mail.yahoo.com> Dorothy: I always used UMBILICAL CORD MEMBRANE as my positive control for Alk-1 (Dako monoclonal at 1:25 HIER at pH8). It is a fantastic (and readily available ) positive control! Ren? J. "Webb, Dorothy L" wrote: Trying this message again, as we still need to come up with a good control for ALK-1!! > -----Original Message----- > From: Webb, Dorothy L > Sent: Tuesday, November 14, 2006 10:52 AM > To: 'Histonet@lists.utsouthwestern.edu' > Subject: controls > > Does anyone know of a possible source to purchase ALK-1 controls? We > cannot seem to come up with a good tissue control and have used > CellMarque as our source, but, they are not available through them at > this time. Appreciate any help in this area and thanks ahead of > time!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Cheap Talk? Check out Yahoo! Messenger's low PC-to-Phone call rates. From superlittlesquirrel <@t> gmail.com Wed Nov 29 09:37:00 2006 From: superlittlesquirrel <@t> gmail.com (Lei Quan) Date: Wed Nov 29 09:37:15 2006 Subject: [Histonet] Casein Question in IHC Message-ID: I had a problem to resolve casein in either PBS or Water when using it for blocking apecific binding. Does anyone have the same problem? Can I use BSA instead or can I heat a bit to help it resolve? Thanks! From Joanne.Malinowski <@t> cambrex.com Wed Nov 29 10:03:26 2006 From: Joanne.Malinowski <@t> cambrex.com (Malinowski, Joanne) Date: Wed Nov 29 10:03:52 2006 Subject: [Histonet] RE: Histonet Digest, Vol 36, Issue 34 Message-ID: Please check references when forwarding information, such as the Microsoft scam below. http://www.snopes.com/inboxer/nothing/billgate.asp Joanne Malinowski Histology Testing Service Cambrex Bio Science Walkersville, Inc. 8830 Biggs Ford Road Walkersville, MD 21793 (301) 898-7025 x 2244 Fax (301) 845-4153 e-mail: joanne.malinowski@cambrex.com -----Original Message----- From: histonet-request@lists.utsouthwestern.edu [mailto:histonet-request@lists.utsouthwestern.edu] Sent: Wednesday, November 29, 2006 9:50 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 36, Issue 34 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Lectins and epithelial cross-reactivity (Milne, Katy) 2. FULL- TIME OPENING FOR HT (Galiotto, Laura) 3. FW: FULL- TIME OPENING FOR HT (Galiotto, Laura) 4. Histotech Fellowship Opportunity (Arzt, Jonathan) 5. Job opening at PhenoPath Laboratories (Patti Loykasek) 6. flourescent microscope (Melody Thiessen) 7. Cytochrome P450 antibody staining (Mark Elliott) 8. RE: Richard Allan (connie grubaugh) 9. re: macrophages/microglia in FFPE tissue (Carl Hobbs) 10. RE: automated immuno stainer (Patsy Ruegg) 11. RE: automated immuno stainer (Weems, Joyce) 12. Missouri Society for Histotechnologists rewards (Rey, Jean-Philippe) 13. SEEKING PAUL HASSEMER (Comcast Mail) 14. Room temperature (donna rossi) 15. Thank you all for your feedback (Heather Renko) 16. weils stain for myelin (stevenk) 17. Re: weils stain for myelin (Bryan Llewellyn) 18. Orcein stain for fat in paraffin sections (Megan Clarke) 19. Anti GFP on cryosections (Adi Sabag) 20. Von Kossa stain (Brunella Spaggiari) 21. FW: controls (Webb, Dorothy L) 22. Fwd: Fwd: FW: READ!!!!!!! (Coleman Manufacturing) 23. Don't fall for the Hoax in a just posted histonet (Lester Raff) 24. RE: Don't fall for the Hoax in a just posted histonet (Dawson, Glen) 25. Re: Fwd: Fwd: FW: READ!!!!!!! (Anne C Lewin) ---------------------------------------------------------------------- Message: 1 Date: Tue, 28 Nov 2006 10:14:49 -0800 From: "Milne, Katy" Subject: [Histonet] Lectins and epithelial cross-reactivity To: Message-ID: <07979E76B0869D4E8C9FE4AA9FC065780240D180@srvex03.phsabc.ehcnet.ca> Content-Type: text/plain; charset="us-ascii" Hi all, I was wondering, speaking of lectins, if anyone has experience using a biotinylated lectin in FFPE mouse tumor tissue (not following perfusion, just routine sectioning) to look at angiogenesis within the tumor. I understand that there could be some cross-reactivity to the epithelial cells which for us would be a bit of a problem as our tumors are pretty solid. Has anyone seen the problem and how bad is it? I'm getting tired of trying to get CD31 and CD34 to behave nicely in our FFPE tumors but I need to be able to see the tumor vasculature. Thanks, Katy Milne Deeley Reseach Centre, BC Cancer Agency, Victoria BC ------------------------------ Message: 2 Date: Tue, 28 Nov 2006 12:37:54 -0600 From: "Galiotto, Laura" Subject: [Histonet] FULL- TIME OPENING FOR HT To: Message-ID: <270614B321ACB44D8C1D91F4F921FDC304B1618F@NCH01EX02.nch.org> Content-Type: text/plain; charset="iso-8859-1" Hello Everyone I have a full-time opening for a Histology Technician, must be ASCP certified. Tues-Sat 3rd shift some flexibility in start time. The hospital is wonderful place to work and excellent benefits. Those that are interested please apply on-line at www.NCH.org Thank you Laura Histology facilitator Northwest Community Hospital Arlington Heights, IL ******************* PLEASE NOTE ******************* This E-Mail/telefax message and any documents accompanying this transmission may contain information that is privileged, confidential, and/or exempt from disclosure under applicable law and is intended solely for the addressee(s) named above. If you are not the intended addressee/recipient, you are hereby notified that any use of, disclosure, copying, distribution, or reliance on the contents of this E-Mail/telefax information is strictly prohibited and may result in legal action against you. Please reply to the sender advising of the error in transmission and immediately delete/destroy the message and any accompanying documents. Thank you. ------------------------------ Message: 3 Date: Tue, 28 Nov 2006 12:42:01 -0600 From: "Galiotto, Laura" Subject: [Histonet] FW: FULL- TIME OPENING FOR HT To: Message-ID: <270614B321ACB44D8C1D91F4F921FDC304B16191@NCH01EX02.nch.org> Content-Type: text/plain; charset="iso-8859-1" > -----Original Message----- > From: Galiotto, Laura > Sent: Tuesday, November 28, 2006 12:38 PM > To: 'HISTONET@LISTS.UTSOUTHWESTERN.EDU' > Subject: FULL- TIME OPENING FOR HT > > Hello Everyone > I have a full-time opening for a Histology Technician, must be ASCP certified. Tues-Sat 3rd shift some flexibility in start time. The hospital is wonderful place to work and excellent benefits. > Those that are interested please apply on-line at www.NCH.org > > Thank you > Laura > Histology facilitator > Northwest Community Hospital > Arlington Heights, IL > ******************* PLEASE NOTE ******************* This E-Mail/telefax message and any documents accompanying this transmission may contain information that is privileged, confidential, and/or exempt from disclosure under applicable law and is intended solely for the addressee(s) named above. If you are not the intended addressee/recipient, you are hereby notified that any use of, disclosure, copying, distribution, or reliance on the contents of this E-Mail/telefax information is strictly prohibited and may result in legal action against you. Please reply to the sender advising of the error in transmission and immediately delete/destroy the message and any accompanying documents. Thank you. ------------------------------ Message: 4 Date: Tue, 28 Nov 2006 14:08:11 -0500 From: "Arzt, Jonathan" Subject: [Histonet] Histotech Fellowship Opportunity To: Message-ID: <6B4AF69268EFAE4C80A786BA99F7A667B41F84@MD-MAIL-01.ARSNET.ARS.USDA.GOV> Content-Type: text/plain; charset="us-ascii" HISTOTECHNOLOGIST RESEARCH FELLOWSHIP OPPORTUNITY - HISTOPATHOLOGY ON FOREIGN ANIMAL DISEASES A unique Histotechnologist research fellowship is currently available working within the pathology division of the USDA, Animal Research Service on Plum Island, NY. We have an immediate opening for a 1-2 year fellow with the possibility of extension beyond the initial fellowship contract. The primary mission of the research group is investigation of veterinary diseases of potential threat to US agriculture interests. Currently the greatest emphasis is directed towards characterizing the pathogenesis of foot-and-mouth disease (aphthovirus). The Plum Island Animal Disease Center is located off the East end of Long Island, NY. Transport to and from the island is provided free to employees aboard government-operated ferries servicing Orient, NY and Old Saybrook, CT. Rural and suburban communities are abundant near both the NY and CT sides of the ferries with access to scenic beaches and vineyards. New York City and Boston are both within 3-4hrs drive. Activities will be varied but will include conventional histotech work, cryosectioning, immunohistochemistry, in-situ hybridization, confocal microscopy, and assistance with animal necropsies and sample collection. The pathology group currently consists of two veterinary pathologists and one other histotech; the group works closely with on-site molecular biologists, immunologists, clinicians, and research fellows. Stipends (ranging from $35,000 to $45,000 a year) will be determined according to education and experience in the field. This fellowship is open to applicants with at least a bachelor's degree and experience in histotechnology. General aspects of the fellowship are described and application is available at: www.orau.gov/piadc For more information, email to the address below. If applying, please notify J. Arzt directly in addition to filing the application with ORISE/ORAU. ******************************************************** Jonathan Arzt DVM, MPVM, DACVP Foreign Animal Disease Research Unit USDA/ARS Plum Island Animal Disease Center P.O. Box 848, Greenport NY 11944 631-323-3171(ph) 631-323-3006(fax) Jonathan.Arzt@ARS.USDA.GOV ******************************************************** ------------------------------ Message: 5 Date: Tue, 28 Nov 2006 11:21:33 -0800 From: Patti Loykasek Subject: [Histonet] Job opening at PhenoPath Laboratories To: histonet Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi all. I would like to announce a job opening at PhenoPath Laboratories in Seattle, WA. The opening is in the clinical IHC division for a part-time technologist (0.75 FTE), day shift. Responsibilities may include performing IHC, IF, molecular testing on patient samples and tissue sectioning (paraffin and frozen). Participation in the development of new tests or technologies is included. Quality control, safety, and preventive maintenance procedures and documentation are required elements of this position as well. Strong preference given to ASCP certified (or eligible) laboratory techs. PhenoPath Laboratories is a national specialty pathology laboratory committed to providing outstanding clinical and research services. We offer an excellent compensation and benefits package, including 401K. Salary will be determined based on the overall skills and background of the chosen applicant. To apply, please e-mail or fax resume & cover letter to: PhenoPath Laboratories 551 North 34th Street, Suite 100 Seattle, WA 98103 Fax: 206.374.9009 Email: ihctech@phenopath.com Web: www.phenopath.com Employment applications available at www.phenopath.com ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. ------------------------------ Message: 6 Date: Tue, 28 Nov 2006 11:27:46 -0800 (PST) From: Melody Thiessen Subject: [Histonet] flourescent microscope To: histonet@lists.utsouthwestern.edu Message-ID: <20061128192746.23061.qmail@web36604.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi there Histonetters! It's been a while, but I have a friend who is in the market for a flourescent microscope; and needs to start pricing them for an equipment grant. She's looking for an upright (light source from the top) possibly with a monitor/video feed as well as the standard eyepieces. She needs UV, red, and blue filters as well. Any leads are appreciated. Thanks! Melody Thiessen ____________________________________________________________________________ ________ Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail beta. http://new.mail.yahoo.com ------------------------------ Message: 7 Date: Tue, 28 Nov 2006 11:36:51 -0800 From: "Mark Elliott" Subject: [Histonet] Cytochrome P450 antibody staining To: Message-ID: <456C1F53020000D60002C069@mail.mrl.ubc.ca> Content-Type: text/plain; charset=US-ASCII Hi Does anyone have any experience staining for cytochrome p450 in paraffin embedded tissues? We are looking at CYP1A1 specifically. We can't seem to get it to stain and are looking for any hints/ideas. We are using human lung tissue. Thanks Mark ***CONFIDENTIALITY NOTICE*** This electronic message is intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication by unauthorized individuals is strictly prohibited. If you have received this communication in error, please notify the sender immediately by reply e-mail and delete the original and all copies from your system. ------------------------------ Message: 8 Date: Tue, 28 Nov 2006 20:47:34 +0000 From: "connie grubaugh" Subject: RE: [Histonet] Richard Allan To: omnivore98@yahoo.com, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; format=flowed I have worked at a few different labs in California and Nevada and every experience with Richard Allen has always been good. Connie Grubaugh Las Vegas Nv. >From: Heather Renko >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Richard Allan Date: Mon, 27 Nov 2006 16:41:14 -0800 >(PST) > >Hello Fellow Histonetters: > > I want to hear the good the bad and the ugly on your experience with >Richard Allan??? > > Concerned Customer > > >--------------------------------- >Everyone is raving about the all-new Yahoo! Mail beta. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ MSN Shopping has everything on your holiday list. Get expert picks by style, age, and price. Try it! http://shopping.msn.com/content/shp/?ctId=8000,ptnrid=176,ptnrdata=200601&tc ode=wlmtagline ------------------------------ Message: 9 Date: Tue, 28 Nov 2006 21:01:16 -0000 From: "Carl Hobbs" Subject: [Histonet] re: macrophages/microglia in FFPE tissue To: "Histonet" Message-ID: <000501c71330$589583a0$0201a8c0@carlba65530bda> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Hi Zach. Perhaps you need to try heat-induced epitope retrieval/unmasking on you pwax sections? I have tried an OX-42 Ab reagent on FFPW sections after the above with negative results. ( and tried various proteolytic enzmes). The only excellent result , for me, was after HIER on pwax rat brain, using an anti-phosphotyrosine Ab reagent. See here for your evaluation; http://www.immunoportal.com/index.php Go to Image gallery and use search box "phosphotyrosine". No guarantees for your species, of course. Good luck. carl ------------------------------ Message: 10 Date: Tue, 28 Nov 2006 14:41:08 -0700 From: "Patsy Ruegg" Subject: RE: [Histonet] automated immuno stainer To: , "'histonet'" , "'Patti Loykasek'" , "'Chistine Tambasco'" Message-ID: <004001c71335$eaf38210$6501a8c0@Patsy> Content-Type: text/plain; charset="us-ascii" I agree with Patti. I have used Ventana, Biogenex and Dako IHC stainers in research and clinical settings. For cost effectiveness, consistent, reliable results I choose the Dako. I have had the same Dako rep for over 10 years and she is great (LuAnne you know who I am talking about). I did not have to pay for the shipping of my instrument and use the instrument based on reagent requisition. We worked together for weeks validating the antibodies I use and they even provided the reagents in some cases. I know Dako has had some troubles of late, but for my money and satisfaction they have and continue to serve me well. Patsy Ruegg -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pathrm35@charter.net Sent: Monday, November 27, 2006 8:49 PM To: histonet; Patti Loykasek; Chistine Tambasco Subject: Re: [Histonet] automated immuno stainer I've used the Ventana, Biogenex and Vision Biosytem Bond IHC stainers. The Vision and Ventana are more user friendly for online dewaxing and antigen retrieval but I really did like the RTU antibodies from Biogenex.They were very consistent and clean staining. Vision Biosytem (Steve Westra in Florida) did have the best technical support though. At one place I worked, the Dako rep wanted to charge us $1,700.00 to ship a demo IHC stainer for us to try. My MD didn't even consider Dako after that. There is no perfect system. You just need to find the one that will fit your needs and budget. I would suggest getting a solid price quote on the equipment, antibodies and other disposable products prior to purchasing. Ron Martin, BS, HT (ASCP) HTL, QIHC ---- Patti Loykasek wrote: > Hi Christine. I believe that a good, well trained tech can achieve good > immunos on any type of platform. I think the main issues are your > preferences: do you want an open or closed system - any detection system or > only the instrument company, concentrated or prediluted antibodies,etc? > Antigen retrieval on instrument or ok off instrument? Cost per slide? Ease > of waste disposal? Can you use the clones of antibodies you have currently > validated on the instrument? User friendly? Those are the main issues I can > think of at the moment - my brain is still tired from all the turkey > tryptophan! > Most companies are more than happy to provide you with a demo & they may > even help work up some things for you. > As for my preference - it is Dako. We have several of the 48 slide > configured Autostainers. I'd be happy to respond to any questions you might > have about the Dako. > Good luck in your stainer hunting. > > > Patti Loykasek BS, HTL, QIHC > PhenoPath Laboratories > Seattle, WA > > > > > > > > > Hello all! I hope everyone enjoyed their breif repreive from stress. I was > > wondering if anyone could give me some input on automated stainers for > > immunohistochemistry. Currently we hand stain and our volumes are steadily > > increasing. We are looking at automation as we probably do up to 12-15 stains > > per day and plan on starting ER and PR. I heard Vantana is very good. How > > about Leica? (Theirs does both immunos and ordianry special stains.) Anyone > > have any experience they would like to share? We are an Ascension Health > > affiliated hospital as that may matter in the negotiations. Thanks for any and > > all opinions. > > Respectfullly, > > Christine Tambasco, HT (ASCP) > > St. Mary's Hospital > > Amsterdam, New York 12010 > > 518-841-7287 > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------------------------------------------------- > This e-mail message, including any attachments, is for the sole use of > the intended recipients and may contain privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If > you are not the intended recipient, please contact the sender by e-mail > and destroy all copies of the original message, or you may call PhenoPath > Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Tue, 28 Nov 2006 16:48:25 -0500 From: "Weems, Joyce" Subject: RE: [Histonet] automated immuno stainer To: "Patsy Ruegg" , , "histonet" , "Patti Loykasek" , "Chistine Tambasco" Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3203708566@sjhaexc02.sjha.org> Content-Type: text/plain; charset="us-ascii" I second! - even with the problems of late, I have always had good service from DAKO - all the way back to the early 80s, when I was very green behind the ears and they walked me through the manual stains holding my hands. They are a very good company. Joyce Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. ------------------------------ Message: 12 Date: Tue, 28 Nov 2006 18:23:53 -0600 From: "Rey, Jean-Philippe" Subject: [Histonet] Missouri Society for Histotechnologists rewards To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello everyone, Does someone know one of these persons? -Bill DeSalvo -Chris Clark Casey -Jerry Fredenbergh -Elvie Taylor -Billie Carney -Ellen Ellis I try to contact them concerning an award their received from the Missouri Society for Histotechnologists between 1991 and 2006. Thanks for you help!! Jean-Philippe Rey Histology Specialist II Histology Core Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 (816) 926-4305 ------------------------------ Message: 13 Date: Tue, 28 Nov 2006 16:42:57 -0800 From: "Comcast Mail" Subject: [Histonet] SEEKING PAUL HASSEMER To: Message-ID: <02af01c7134f$52f04290$71f9c147@DFC4J821> Content-Type: text/plain; charset="iso-8859-1" Paul Hassemer, please contact me at your convenience. Thanks. Tina Yates, HT(ASCP) Sr. Lab Specialist Salem Hospital Regional Laboratory P: 503.561.5443 F: 503.561.4781 tina.yates@salemhospital.org ------------------------------ Message: 14 Date: Tue, 28 Nov 2006 20:11:36 -0500 From: "donna rossi" Subject: [Histonet] Room temperature To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; format="flowed" Hi Everyone in Histoland! Can someone please tell me what is the correct room temperature range for a histology lab that has an autoimmunostainer located within it. Also, at what temperature should paraffin blocks be stored at? Thanks, Donna ,SRHS [emwink.gif] donnarossi [emcocktl.gif] _________________________________________________________________ [1]Stay up-to-date with your friends through the Windows Live Spaces friends list. References 1. http://g.msn.com/8HMBENUS/2737??PS=47575 ------------------------------ Message: 15 Date: Tue, 28 Nov 2006 17:31:15 -0800 (PST) From: Heather Renko Subject: [Histonet] Thank you all for your feedback To: histonet@lists.utsouthwestern.edu Message-ID: <20061129013115.76267.qmail@web31310.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 To all, thank you for your positive feedback regarding RA. It really helped me with my perspective on service for capital equipment. Heather --------------------------------- Access over 1 million songs - Yahoo! Music Unlimited. ------------------------------ Message: 16 Date: Wed, 29 Nov 2006 15:39:51 +1100 From: stevenk Subject: [Histonet] weils stain for myelin To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="us-ascii" ; format="flowed" I am asking for a colleague who was doing a Weil's stain for myelin. One of the ingredients, 2% alcoholic haematoxylin requires a ripening process for at least 6 months. As the student cannot wait that long, I was wondering if anyone could supply a quicker method, or suggest something to hasten the ripening process of the above. Thanks from down under Stephen -- |Stephen Kum Jew |Senior Technical Officer |Discipline of Pathology |School of Medical Sciences |Blackburn Building D06 |University of Sydney NSW 2006 |Australia |Ph: + 61 2 9351 6143 |Fax:+61 2 9351 3429 ------------------------------ Message: 17 Date: Tue, 28 Nov 2006 21:54:35 -0800 From: Bryan Llewellyn Subject: Re: [Histonet] weils stain for myelin To: Histonet Message-ID: <000d01c7137a$d936bed0$130e4246@yourlk4rlmsu> Content-Type: text/plain; format=flowed; charset=iso-8859-1; reply-type=response Make up a fresh solution and just dust a little sodium iodate into it, but not too much. Leave half an hour and use. Alternatively, use a fresh solution and leave it ten minutes after adding the mordant. It will ripen OK. The method is done by inspection anyway, so just keep an eye on the staining and adjust the times. Bryan Llewellyn ----- Original Message ----- From: "stevenk" To: Sent: Tuesday, November 28, 2006 8:39 PM Subject: [Histonet] weils stain for myelin > > I am asking for a colleague who was doing a Weil's stain for myelin. > > One of the ingredients, 2% alcoholic haematoxylin requires a ripening > process for at least 6 months. > > As the student cannot wait that long, I was wondering if anyone could > supply a quicker method, or suggest something to hasten the ripening > process of the above. > > Thanks from down under > > Stephen > -- > |Stephen Kum Jew > |Senior Technical Officer > |Discipline of Pathology > |School of Medical Sciences > |Blackburn Building D06 > |University of Sydney NSW 2006 > |Australia > |Ph: + 61 2 9351 6143 > |Fax:+61 2 9351 3429 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 18 Date: Wed, 29 Nov 2006 18:23:31 +1100 From: "Megan Clarke" Subject: [Histonet] Orcein stain for fat in paraffin sections To: Message-ID: Content-Type: text/plain; charset=US-ASCII Hi All Hope that someone out there has this old stain procedure, an Orcein stain to stain for fat in paraffin sections. We had tissue erronously placed in formalin and subsequently processed. The pathologist would like to demonstrate fat using the orcein method. Thanks Zenobia Haffajee HAPS, IHC, Anatomical pathology Newcastle Australia. ------------------------------ Message: 19 Date: Wed, 29 Nov 2006 12:40:06 +0200 From: Adi Sabag Subject: [Histonet] Anti GFP on cryosections To: Histonet Message-ID: <1164796806.456d6386dedc5@webmail.technion.ac.il> Content-Type: text/plain; charset=Windows-1255 Hi All, Does anyone have an experience with anti-GFP antibody of MBL on cryosections? Thanks- Adi -- Adi Sabag Tumor Progression and Angiogenesis laboratory Medicine Faculty, Technion Haifa, Israel. 972-4-8295425 ------------------------------ Message: 20 Date: Wed, 29 Nov 2006 12:20:59 +0100 From: "Brunella Spaggiari" Subject: [Histonet] Von Kossa stain To: "Histonet" Message-ID: <003301c713a8$72cd5040$ba3a4ea0@PC186Gabbi> Content-Type: text/plain; charset="iso-8859-1" We work on undelcalcified bone tissue (human, rabbit) fixed in PFA 4% and embedded in methylmethacrilate (MMA). >From our samples we obtain 50 m thick sections which can contain titanium implants. We tried to perform Von Kossa stain (see attachment), always on undeplasticized sections, but we met several problems: - calcium deposits after silver nitrate+UV light did not appear black but brown, and, after sodium thiosulphate, brown stain turns more and more light; - we could not find a suitable counterstain for osteoid: nuclear fast red and neutral red do not stain at all, basic or acid fuchsin stain too much and cover the underlying brown.. Some suggestions? Are there other good histological stainings for undeplasticized bone sections in your experience? Thank you! Brunella ------------------------------ Message: 21 Date: Wed, 29 Nov 2006 06:19:36 -0600 From: "Webb, Dorothy L" Subject: [Histonet] FW: controls To: Histonet@lists.utsouthwestern.edu Message-ID: <0E394B648E5284478A6CCB78E5AFDA27036407AC@hpes1.HealthPartners.int> Content-Type: text/plain; charset="us-ascii" Trying this message again, as we still need to come up with a good control for ALK-1!! > -----Original Message----- > From: Webb, Dorothy L > Sent: Tuesday, November 14, 2006 10:52 AM > To: 'Histonet@lists.utsouthwestern.edu' > Subject: controls > > Does anyone know of a possible source to purchase ALK-1 controls? We > cannot seem to come up with a good tissue control and have used > CellMarque as our source, but, they are not available through them at > this time. Appreciate any help in this area and thanks ahead of > time!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. ------------------------------ Message: 22 Date: Wed, 29 Nov 2006 05:31:20 -0800 (PST) From: Coleman Manufacturing Subject: [Histonet] Fwd: Fwd: FW: READ!!!!!!! To: histonet@lists.utsouthwestern.edu Message-ID: <20061129133120.96227.qmail@web37606.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Date: Tue, 28 Nov 2006 23:17:46 -0500 From: "Sara L. Browder" To: coleman_manufacturing@yahoo.com Subject: Fwd: Fwd: FW: READ!!!!!!! ----- Forwarded message from steelea3@mailbox.sc.edu ----- Date: Tue, 28 Nov 2006 20:31:49 -0500 From: steelea3@mailbox.sc.edu Reply-To: steelea3@mailbox.sc.edu Subject: Fwd: FW: READ!!!!!!! To: browdesl@mailbox.sc.edu ----- Forwarded message from kkyle lavette ----- Date: Wed, 29 Nov 2006 00:56:44 +0000 From: kkyle lavette Reply-To: kkyle lavette Subject: FW: READ!!!!!!! To: bucablack2@aol.com, MusicalSMJ@aol.com, robtbarton@aol.com, DonnaB210@aol.com, braziel@webmail.sc.edu, xxraiderxx72@hotmail.com, conleydarrick@yahoo.com, daiglep@mailbox.sc.edu, DAVISKE@gwm.sc.edu, johnson@cadetmail.uscga.edu, canesnj8@aol.com, ashkirk09@yahoo.com, G_Knight_21@hotmail.com, jimv@cox.net, jpvasellina@hotmail.com, jvas@bellsouth.net, STEELEA3@mailbox.sc.edu, napole5127@aol.com, rose.shores@pw.utc.com, kels1021@hotmail.com, mike787b@hotmail.com, johnnyv@ntplx.net, Xaviar3@aol.com, mckier@mailbox.sc.edu, idodough@hotmail.com, dlavette@ofalaw.com >From: HANDKL@mailbox.sc.edu >To: HANDKL@mailbox.sc.edu >Subject: READ!!!!!!! >Date: Mon, 27 Nov 2006 22:12:26 -0500 (EST) > >Read carefully... > > >THIS TOOK TWO PAGES OF THE TUESDAY USA TODAY - IT IS FOR REAL > >To all of my friends, I do not usually forward messages, >But this is from my friend Pearlas Sandborn and she really is >an attorney. > >If she says that this will work - It will work. After all,What have >you got to lose? > >SORRY EVERYBODY.. JUST HAD TO TAKE THE CHANCE!!! I'm an >attorney, And I know the law. This thing is for real. Rest assured >AOL and &nbs p; Intel will follow through with their promises for >fear of facing a multimillion-dollar class action suit similar to the one >filed by PepsiCo against General Electric not too long ago. > >Dear Friends: Please do not take this for a junk letter. >Bill Gates sharing his fortune. If you ignore this, You will repent >later. > >Microsoft and AOL are now the largest Internet companies >and in an effort to make sure that Internet Explorer remains the >most widely used program, Microsoft and AOL are running an e-mail >beta test. > >When you forward this e-mail to friends, Microsoft can and will >track it (If you are a Microsoft Windows user) For a two weeks >time period. > >For every person that you forward this e-mail to, Microsoft will pay >you $245.00 For every person that you sent it to that forwards it on, >Microsoft will pay you $243.00 and for every third person that receives >it, You will be paid $241.00. Within two weeks, Microsoft will contact >you for your address and then send you a check. > >Regards. Charles S Bailey General Manager Field Operations >1-800-842-2332 Ext. 1085 or 904-1085 or RNX 292-1085 > > >Thought this was a scam myself, But two weeks after receiving this >e-mail and forwarding it on. Microsoft contacted me for my address and >within days, I received a check for $24, 800.00. You need to respond >before the beta testing is over. If anyone can affoard this, Bill gates is >the >man. > >It's all marketing expense to him. Please forward this to as many >people as possible. You are bound to get at least $10, 000.00 >We're not going to help them out with their e-mail beta test without >getting a little something for our time. My brother's girlfriend got in >on this a few months ago. When I went to visit him for the Baylor/UT >game, she showed me her check. It was for the sum of $4, 324.44 and >was stamped "Paid In Full". > > > > > > > > > > >_____________________ Confidentiality _____________________ > > > >This electronic transmission is strictly confidential and intended > >solely for the addressee. It may contain information which is covered > >by legal, professional or other privilege. If you are not the intended > >addressee, you must not disclose, copy or take any action in reliance > >of this transmission. If you have received this transmission in error, > >please notify us and delete the received data as soon as possible. > > > >This footnote also confirms that this email message has been swept > >for the presence of computer viruses. > >_______________________________________________________ > > > > > > > > Adalgiza "ADA" Storto > >A & D SupportWear Inc. > >5919 Greenville Ave. #380 > > Dallas,TX 75206 > > 214-883-7373 > > office: 214-363-1600 / 972- 414- 3701 > > fax: 214-265-7655 / 972- 414-3718 > >e-mail: adarx2000@yahoo.com > > > > > > > > > >--------------------------------------------------------------------------- --- - - > > > > > >IMPORTANT: The sender intends that this electronic message is for >the exclusive use of the person to whom it is addressed. This >message may contain information that is confidential. If the reader >of this message is not an intended recipient, be aware that any >disclosure, dissemination, distribution or copying of this >communication, or the use of its contents, is prohibited. If you >have received this message in error, then please immediately notify >the sender of your inadvertent receipt and delete this message from >all data storage systems. Thank you. _________________________________________________________________ Fixing up the home? Live Search can help http://imagine-windowslive.com/search/kits/default.aspx?kit=improve&locale=e n- US&source=hmemailtaglinenov06&FORM=WLMTAG ----- End forwarded message ----- ----- End forwarded message ----- Sara L. Browder ------------------------------ Message: 23 Date: Wed, 29 Nov 2006 07:58:33 -0600 From: "Lester Raff" Subject: [Histonet] Don't fall for the Hoax in a just posted histonet To: Message-ID: <5DA1CA5D0B98A84985B545A24423B822019ADA@UPLAB01.uplab.local> Content-Type: text/plain; charset="us-ascii" See below before you expect any of Bill Gates' money. http://www.hoax-slayer.com/ms-money-giveway-hoax.html Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 ------------------------------ Message: 24 Date: Wed, 29 Nov 2006 08:17:36 -0600 From: "Dawson, Glen" Subject: RE: [Histonet] Don't fall for the Hoax in a just posted histonet To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" It never ceases to amaze that people still fall for this garbage. WOW. I just wish they would come up with something more original like an upcoming diamond meteor shower or a new laser that turns used kleenex into gold. Gulliblely Yours, Glen Dawson Milwaukee, WI ------------------------------ Message: 25 Date: Wed, 29 Nov 2006 09:34:43 -0500 From: Anne C Lewin Subject: Re: [Histonet] Fwd: Fwd: FW: READ!!!!!!! To: Coleman Manufacturing Cc: histonet@lists.utsouthwestern.edu Message-ID: <456D9A83.1040207@bms.com> Content-Type: text/plain; charset="iso-8859-1" *This is a hoax*, started in 1997 for more info, visit Snopes.com, they are great when it comes to de-mything urban legends...... http://www.snopes.com/inboxer/nothing/microsoft-aol.asp Coleman Manufacturing wrote: > Date: Tue, 28 Nov 2006 23:17:46 -0500 >From: "Sara L. Browder" >To: coleman_manufacturing@yahoo.com >Subject: Fwd: Fwd: FW: READ!!!!!!! > > > >----- Forwarded message from steelea3@mailbox.sc.edu ----- >Date: Tue, 28 Nov 2006 20:31:49 -0500 >From: steelea3@mailbox.sc.edu >Reply-To: steelea3@mailbox.sc.edu >Subject: Fwd: FW: READ!!!!!!! >To: browdesl@mailbox.sc.edu > > > >----- Forwarded message from kkyle lavette ----- >Date: Wed, 29 Nov 2006 00:56:44 +0000 >From: kkyle lavette >Reply-To: kkyle lavette >Subject: FW: READ!!!!!!! >To: bucablack2@aol.com, MusicalSMJ@aol.com, robtbarton@aol.com, >DonnaB210@aol.com, braziel@webmail.sc.edu, xxraiderxx72@hotmail.com, >conleydarrick@yahoo.com, daiglep@mailbox.sc.edu, DAVISKE@gwm.sc.edu, >johnson@cadetmail.uscga.edu, canesnj8@aol.com, ashkirk09@yahoo.com, >G_Knight_21@hotmail.com, jimv@cox.net, jpvasellina@hotmail.com, >jvas@bellsouth.net, STEELEA3@mailbox.sc.edu, napole5127@aol.com, >rose.shores@pw.utc.com, kels1021@hotmail.com, mike787b@hotmail.com, >johnnyv@ntplx.net, Xaviar3@aol.com, mckier@mailbox.sc.edu, >idodough@hotmail.com, dlavette@ofalaw.com > > > > > > >>From: HANDKL@mailbox.sc.edu >>To: HANDKL@mailbox.sc.edu >>Subject: READ!!!!!!! >>Date: Mon, 27 Nov 2006 22:12:26 -0500 (EST) >> >>Read carefully... >> >> >>THIS TOOK TWO PAGES OF THE TUESDAY USA TODAY - IT IS FOR REAL >> >>To all of my friends, I do not usually forward messages, >>But this is from my friend Pearlas Sandborn and she really is >>an attorney. >> >>If she says that this will work - It will work. After all,What have >>you got to lose? >> >>SORRY EVERYBODY.. JUST HAD TO TAKE THE CHANCE!!! I'm an >>attorney, And I know the law. This thing is for real. Rest assured >>AOL and &nbs p; Intel will follow through with their promises for >>fear of facing a multimillion-dollar class action suit similar to the one >>filed by PepsiCo against General Electric not too long ago. >> >>Dear Friends: Please do not take this for a junk letter. >>Bill Gates sharing his fortune. If you ignore this, You will repent >>later. >> >>Microsoft and AOL are now the largest Internet companies >>and in an effort to make sure that Internet Explorer remains the >>most widely used program, Microsoft and AOL are running an e-mail >>beta test. >> >>When you forward this e-mail to friends, Microsoft can and will >>track it (If you are a Microsoft Windows user) For a two weeks >>time period. >> >>For every person that you forward this e-mail to, Microsoft will pay >>you $245.00 For every person that you sent it to that forwards it on, >>Microsoft will pay you $243.00 and for every third person that receives >>it, You will be paid $241.00. Within two weeks, Microsoft will contact >>you for your address and then send you a check. >> >>Regards. Charles S Bailey General Manager Field Operations >>1-800-842-2332 Ext. 1085 or 904-1085 or RNX 292-1085 >> >> >>Thought this was a scam myself, But two weeks after receiving this >>e-mail and forwarding it on. Microsoft contacted me for my address and >>within days, I received a check for $24, 800.00. You need to respond >>before the beta testing is over. If anyone can affoard this, Bill gates is >>the >>man. >> >>It's all marketing expense to him. Please forward this to as many >>people as possible. You are bound to get at least $10, 000.00 >>We're not going to help them out with their e-mail beta test without >>getting a little something for our time. My brother's girlfriend got in >>on this a few months ago. When I went to visit him for the Baylor/UT >>game, she showed me her check. It was for the sum of $4, 324.44 and >>was stamped "Paid In Full". >> >> >> >> >> >> >> >> >> >> >>_____________________ Confidentiality _____________________ >> >> >> >>This electronic transmission is strictly confidential and intended >> >>solely for the addressee. It may contain information which is covered >> >>by legal, professional or other privilege. If you are not the intended >> >>addressee, you must not disclose, copy or take any action in reliance >> >>of this transmission. If you have received this transmission in error, >> >>please notify us and delete the received data as soon as possible. >> >> >> >>This footnote also confirms that this email message has been swept >> >>for the presence of computer viruses. >> >>_______________________________________________________ >> >> >> >> >> >> >> >>Adalgiza "ADA" Storto >> >>A & D SupportWear Inc. >> >>5919 Greenville Ave. #380 >> >>Dallas,TX 75206 >> >>214-883-7373 >> >>office: 214-363-1600 / 972- 414- 3701 >> >>fax: 214-265-7655 / 972- 414-3718 >> >>e-mail: adarx2000@yahoo.com >> >> >> >> >> >> >> >> >> >>-------------------------------------------------------------------------- ---- >> >> >- >- > > >> >> >> >>IMPORTANT: The sender intends that this electronic message is for >>the exclusive use of the person to whom it is addressed. This >>message may contain information that is confidential. If the reader >>of this message is not an intended recipient, be aware that any >>disclosure, dissemination, distribution or copying of this >>communication, or the use of its contents, is prohibited. If you >>have received this message in error, then please immediately notify >>the sender of your inadvertent receipt and delete this message from >>all data storage systems. Thank you. >> >> > >_________________________________________________________________ >Fixing up the home? Live Search can help >http://imagine-windowslive.com/search/kits/default.aspx?kit=improve&locale= en- >US&source=hmemailtaglinenov06&FORM=WLMTAG > > >----- End forwarded message ----- > > > >----- End forwarded message ----- > > >Sara L. Browder > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 36, Issue 34 **************************************** From hborgeri <@t> wfubmc.edu Wed Nov 29 10:09:43 2006 From: hborgeri <@t> wfubmc.edu (Hermina Borgerink) Date: Wed Nov 29 10:52:33 2006 Subject: [Histonet] Casein Question in IHC Message-ID: <9AEEF1FB6254224AA355ED285F8491651D854B02@EXCHVS2.medctr.ad.wfubmc.edu> Hi Lei, I routinely use 0.5% casein in Tris buffer for all my antibody diluents and washes. After adding the appropriate number of grams to the buffer, I leave the solution stirring overnight with the aid of a stir plate and stir bar. Although the solution continues to have a cloudy aspect the following morning, all the casein has actually gone into solution. Ref.: Casein Reduces Nonspecific Background Staining in Immunolabeling Techniques by David E. Tacha et al J. Histotechnology, 1992 15: 2, p. 127 Hermina -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lei Quan Sent: Wednesday, November 29, 2006 10:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Casein Question in IHC I had a problem to resolve casein in either PBS or Water when using it for blocking apecific binding. Does anyone have the same problem? Can I use BSA instead or can I heat a bit to help it resolve? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jessica <@t> medstaffservices.com Wed Nov 29 12:43:33 2006 From: Jessica <@t> medstaffservices.com (Jessica Hirsch) Date: Wed Nov 29 12:43:50 2006 Subject: [Histonet] please post to digest Message-ID: CURRENT EMPLOYMENT OPPROTUNITIES ( HISTOLOGY, IMMUNOHISTOCHEMISTRY & PATHOLOGY ) I. Clarksburg, Maryland (Large Nationwide Clinical Labratory (division of BioReference)) 1. Histology Supervisor: Hours: Monday - Friday 12 midnight - 8:30am Pay: $25/hr + 10% night differential = $27.50/hr Benefits: Medical, Dental, 401k 2. Histology Technologist Hours: Monday - Friday 12 midnight - 8:30am Pay: $25/hr + 10% night differential = $27.50/hr Benefits: Medical, Dental, 401k 3. Grossers Hours: Monday - Friday 7pm-11:30pm Pay: $30/hr II. Elmwood Park, New Jersey ( Nationwide Clinical Laboratory) 1. Histology Technologist ( 2 positions available, immediate start ) Hours: Monday - Friday, 9:00pm - 5:30am Pay: Negotiable Benefits: Medical, Dental, 401k 2. ImmunoHistoChemistry Supervisor Hours: Monday - Friday, 7am or 8am - 4pm or 5pm Pay: Negotiable Benefits: Medical, Dental, 401k III. New York City, New York ( large world renowned Cancer Hospital ) 1. Pathologist Assistant Hours: Monday - Friday, Day shift, Full-Time Pay: $60k - $80k based on experience Benefits: Major Medical, Dental, Vision, 401k, etc. 2. Histology Technologist Hours: Monday - Friday (shift to be announced) Pay: negotiable Benefits: Medical, Dental, 401k For immediate consideration for any position: Send resumes to Jessica@medstaffservices.com OR fax to 732.238.2152 For questions call: Jessica Hirsch at 732.238.6050 ext.58 Jessica D. Hirsch Staffing Specialist Medical Staffing Services, Inc. 557 Cranbury Road East Brunswick, NJ 08816 P: (732) 238-6050 x58 F: (732) 238-2152 Jessica@medstaffservices.com http://www.medstaffservices.com From Jason.Wiese <@t> va.gov Wed Nov 29 13:17:43 2006 From: Jason.Wiese <@t> va.gov (Wiese, Jason VHAROS) Date: Wed Nov 29 13:17:55 2006 Subject: [Histonet] non-pathologist grossing procedure Message-ID: <70EEF3D43B3C164C94037D811B2BE193E1265D@VHAV20MSGA3.v20.med.va.gov> Hello Histonetters: I am in the process of rewriting procedure manuals that are so outdated, some procedures were written before I was born! Anyway, an help with the following questions would be greatly appreciated. I am looking for electronic files, preferably in WORD that can be emailed to me. CAP Checklist ANP.11620 Phase II Are the types of specimens examined and the extent of the examination performed by a non-pathologist clearly defined in a documented policy or protocol? Anp.11630 Phase II Is the nature of the pathologist supervision (direct vs. indirect) clearly documented for each type of specimen grossly examined by a non-pathologist? How do we go about this? Do any of you have checklists/protocols/policies? What exactly does CAP want? Please help!! Thank You! Jason E. Wiese VAROS Pathology Histology/Cytology 913 NW Garden Valley Blvd Roseburg, OR 97470 Jason.wiese@med.va.gov 541-440-1000 Ext. 44751 From Jason.Wiese <@t> va.gov Wed Nov 29 13:17:59 2006 From: Jason.Wiese <@t> va.gov (Wiese, Jason VHAROS) Date: Wed Nov 29 13:18:12 2006 Subject: [Histonet] Thermo Electron Message-ID: <70EEF3D43B3C164C94037D811B2BE193E1265E@VHAV20MSGA3.v20.med.va.gov> I will try one more time... My messages always get bounced back to me for some reason, but I thought I would try one more time before I unsubscribe to this network. I have yet to be able to respond or send messages to you guys unless I directly respond to a posting. Anyway.... I just purchased a Thermo Electron Excelsior processor, a Gemini stainer, and a Cryotome FE. I need to update procedure manuals for these pieces of equipment. I am hoping someone in histoland can help me. I can write an entire procedure for the operation of each piece of equipment, but it would be a great deal of help if someone could email me their procedure for any of these products so I have something to work from. Thank You So Much... Jason E. Wiese, BS, HT(ASCP) 913 NW Garden Valley Blvd Roseburg, OR 97470 541-440-1000 Ext. 44751 jason.wiese@med.va.gov From mtarango <@t> nvcancer.org Wed Nov 29 13:46:27 2006 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Wed Nov 29 13:47:06 2006 Subject: [Histonet] Richard Allan Message-ID: <5AEC610C1CE02945BD63A395BA763EDEC9AD13@NVCIEXCH02.NVCI.org> I've never had a problem with them. I've found them to be better than most companies. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of connie grubaugh Sent: Tuesday, November 28, 2006 12:48 PM To: omnivore98@yahoo.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Richard Allan I have worked at a few different labs in California and Nevada and every experience with Richard Allen has always been good. Connie Grubaugh Las Vegas Nv. >From: Heather Renko >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Richard Allan Date: Mon, 27 Nov 2006 16:41:14 -0800 >(PST) > >Hello Fellow Histonetters: > > I want to hear the good the bad and the ugly on your experience with >Richard Allan??? > > Concerned Customer > > >--------------------------------- >Everyone is raving about the all-new Yahoo! Mail beta. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ MSN Shopping has everything on your holiday list. Get expert picks by style, age, and price. Try it! http://shopping.msn.com/content/shp/?ctId=8000,ptnrid=176,ptnrdata=20060 1&tcode=wlmtagline _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "MMS " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From SecrestK <@t> wvuh.com Wed Nov 29 14:21:35 2006 From: SecrestK <@t> wvuh.com (Secrest, Kimberly) Date: Wed Nov 29 14:22:05 2006 Subject: [Histonet] Fixation and Immunos Message-ID: <7708E0A8E46BDC40B23113F97FB0FB23031DCD74@nt-exchange1.wvuh.wvuhs.com> The possibly of using alcohol/formalin combination fixatives, Pen-fix has been brought up by pathologists and PAs, but there is also concern about the effects the fixative may have on immuno staining. We have two XTs and Ventana has only tested with 10% formalin. They did suggest that with alcoholic fixatives the cell conditioning may need to be decreased. Does anyone use these fixatives on tissue where immunos are done, particularly with ventana products? Do you notice any negative effects? What about in situ hybridization? Thanks Kimberly Secrest HTL, QIHC Histology Technical Specialist West Virginia University Hospital From liz <@t> premierlab.com Wed Nov 29 14:54:32 2006 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Nov 29 14:46:27 2006 Subject: [Histonet] control block for Pseudomonas aeruginosa Message-ID: <000a01c713f8$922a95b0$0c00a8c0@domain.Premier> Hello All I need to locate a block of human Cystic Fibrosis lung positive for P.aeruginosa. Does anyone know where I could get something like this. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 From hymclab <@t> hyhc.com Wed Nov 29 14:54:24 2006 From: hymclab <@t> hyhc.com (hymclab) Date: Wed Nov 29 14:50:43 2006 Subject: [Histonet] non-pathologist grossing procedure Message-ID: Please post to list as we are in the process have changing to have our HT's gross in biopsy specs and I will need to write new procedures for this to comply with CAP. Thanks, Dawn D. Schneider, HT(ASCP) Howard Young Medical Center Woodruff, WI 54568 (715)356-8174 schneiderd@hyhc.com -----Original Message----- From: Wiese, Jason VHAROS [mailto:Jason.Wiese@va.gov] Sent: Wednesday, November 29, 2006 1:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] non-pathologist grossing procedure Hello Histonetters: I am in the process of rewriting procedure manuals that are so outdated, some procedures were written before I was born! Anyway, an help with the following questions would be greatly appreciated. I am looking for electronic files, preferably in WORD that can be emailed to me. CAP Checklist ANP.11620 Phase II Are the types of specimens examined and the extent of the examination performed by a non-pathologist clearly defined in a documented policy or protocol? Anp.11630 Phase II Is the nature of the pathologist supervision (direct vs. indirect) clearly documented for each type of specimen grossly examined by a non-pathologist? How do we go about this? Do any of you have checklists/protocols/policies? What exactly does CAP want? Please help!! Thank You! Jason E. Wiese VAROS Pathology Histology/Cytology 913 NW Garden Valley Blvd Roseburg, OR 97470 Jason.wiese@med.va.gov 541-440-1000 Ext. 44751 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From Jason.Wiese <@t> va.gov Wed Nov 29 15:16:44 2006 From: Jason.Wiese <@t> va.gov (Wiese, Jason VHAROS) Date: Wed Nov 29 15:16:53 2006 Subject: [Histonet] non-pathologist grossing procedure In-Reply-To: Message-ID: <70EEF3D43B3C164C94037D811B2BE193E12661@VHAV20MSGA3.v20.med.va.gov> Dawn: As soon as I have something I will post it. I myself have been taking on the gross of more and more specimens, and although I have direct supervision should I need it, I need a clear policy/procedure for any specimens grossed by anyone other then a pathologist. Best Regards, Jason Wiese, BS, HT(ASCP) -----Original Message----- From: Schneider, Dawn [mailto:SchneiderD@hyhc.com] On Behalf Of hymclab Sent: Wednesday, November 29, 2006 12:54 PM To: Wiese, Jason VHAROS; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] non-pathologist grossing procedure Please post to list as we are in the process have changing to have our HT's gross in biopsy specs and I will need to write new procedures for this to comply with CAP. Thanks, Dawn D. Schneider, HT(ASCP) Howard Young Medical Center Woodruff, WI 54568 (715)356-8174 schneiderd@hyhc.com -----Original Message----- From: Wiese, Jason VHAROS [mailto:Jason.Wiese@va.gov] Sent: Wednesday, November 29, 2006 1:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] non-pathologist grossing procedure Hello Histonetters: I am in the process of rewriting procedure manuals that are so outdated, some procedures were written before I was born! Anyway, an help with the following questions would be greatly appreciated. I am looking for electronic files, preferably in WORD that can be emailed to me. CAP Checklist ANP.11620 Phase II Are the types of specimens examined and the extent of the examination performed by a non-pathologist clearly defined in a documented policy or protocol? Anp.11630 Phase II Is the nature of the pathologist supervision (direct vs. indirect) clearly documented for each type of specimen grossly examined by a non-pathologist? How do we go about this? Do any of you have checklists/protocols/policies? What exactly does CAP want? Please help!! Thank You! Jason E. Wiese VAROS Pathology Histology/Cytology 913 NW Garden Valley Blvd Roseburg, OR 97470 Jason.wiese@med.va.gov 541-440-1000 Ext. 44751 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From AnthonyH <@t> chw.edu.au Wed Nov 29 16:26:33 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Nov 29 16:28:26 2006 Subject: [Histonet] weils stain for myelin Message-ID: Weil's Hx is made up similarly to Verhoeff's Elastic Stain Hx. Staining with Verhoeff's is clearer and easier to differentiate if aged alcoholic Hx is used. If you have any alcoholic HX that has been prepared as a stock for another technique, you can use it in the Weil's method. I dilute it with ethanol to the required concentration (we have two stock 10% Hx in ethanol solutions available and as one is used, the other has ripened and is ready for use). Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of stevenk Sent: Wednesday, 29 November 2006 3:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] weils stain for myelin I am asking for a colleague who was doing a Weil's stain for myelin. One of the ingredients, 2% alcoholic haematoxylin requires a ripening process for at least 6 months. As the student cannot wait that long, I was wondering if anyone could supply a quicker method, or suggest something to hasten the ripening process of the above. Thanks from down under Stephen -- |Stephen Kum Jew |Senior Technical Officer |Discipline of Pathology |School of Medical Sciences |Blackburn Building D06 |University of Sydney NSW 2006 |Australia |Ph: + 61 2 9351 6143 |Fax:+61 2 9351 3429 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From lorimroberts2 <@t> yahoo.com Wed Nov 29 16:40:44 2006 From: lorimroberts2 <@t> yahoo.com (Lori Roberts) Date: Wed Nov 29 16:40:50 2006 Subject: [Histonet] hand processing of rat brain revisited Message-ID: <315915.91234.qm@web34312.mail.mud.yahoo.com> Hi there, I need some advice on a hand processing schedule for rat brains. Because I am the only person at my company with histology experience (limited to some paraffin embedding and sectioning I did of embryonic tissues in grad school) I have been asked to set up a histology lab from scratch on short notice. We are trying to replicate an experiment that was done at a contract lab, and we need results before the end of the year. The contract lab provided us with all of their protocols, but of course they use automated processing, with vacuum at every step. I am processing cassettes like I did in school--in a polypropylene tri-pour beaker that I burned holes into inside another beaker with a stirbar in the outer beaker (no vacuum). The paraffin steps are carried out in a vacuum oven at 58C/-15 mm Hg vacuum. When I tried to process some practice brains, the sectioning was terrible--brittle tissue and jammed up sections. One problem is that I used too small a volume of fix--only 20 mL before reading in Frieda Carson's Histotechnology text that I should use at least 20X volume (like I said, my only experience was with tiny tissues where volume was never an issue). I am fixing some new brains in 60 mL NBF per brain. But before processing these I thought I'd get some professional opinions about whether the schedules I have tried are appropriate for rat brain. Here are the two schedules I tried: Contract lab's schedule (they use this with vacuum at every step, I have vacuum only for paraffin): Whole rat brain fixed 10% NBF (20 mL) 36 hours Trimmed into 3 mm slices of forebrain and midbrain, placed in cassettes and stored O/N in formalin 30 min 70% EtOH 30 min 80% EtOH 30 min 95% EtOH times two 30 min 100% EtOH times three 45 min xylenes times two 40 min ParaPlast Xtra 58C/-15 mm Hg times two 80 min ParaPlast Xtra 58C/-15 mm Hg After these didn't section well, I took some remaining pieces of the above fixed brains to try the schedule I used to use in graduate school for embryonic tissues: Leftover brain pieces from first try stored in same 20 mL NBF vial as above 7 days Trimmed into 3 mm slices, placed in cassettes and immediately processed 45 min 70% EtOH 45 min 95% EtOH 60 min 100% EtOH 45 min xylenes times three 60 min ParaPlast Xtra 58C/-15 mm Hg times two I have ordered an inexpensive Nalgene vacuum chamber so I will soon be able to apply vacuum at the earlier dehydration steps. My next question concerns the vacuum pressure. I have seen values in the Histonet archives ranging from -15 mm Hg to 40-200 mm Hg to 15 inches Hg. The gauge on my vacuum oven only goes down to -30 mm Hg. How much vacuum should I be using? Sorry for the long message, but I know the devil's in the details. Thank you, Lori Roberts --------------------------------- Access over 1 million songs - Yahoo! Music Unlimited. From stevenk <@t> med.usyd.edu.au Wed Nov 29 19:22:29 2006 From: stevenk <@t> med.usyd.edu.au (stevenk) Date: Wed Nov 29 19:21:31 2006 Subject: [Histonet] Re:Weils Message-ID: Many thanks for the advice on the above. Stephen -- |Stephen Kum Jew |Senior Technical Officer |Discipline of Pathology |School of Medical Sciences |Blackburn Building D06 |University of Sydney NSW 2006 |Australia |Ph: + 61 2 9351 6143 |Fax:+61 2 9351 3429 From graham.brown <@t> ttuhsc.edu Wed Nov 29 19:54:44 2006 From: graham.brown <@t> ttuhsc.edu (Brown, Graham W) Date: Wed Nov 29 19:54:56 2006 Subject: [Histonet] Thrombin staining Message-ID: <7F7DE44DEE269C4CB34BB8EADE0525EC61E46E@BOWIE.ttuhsc.edu> Has anyone succesfully stained for Thrombin in formalin fixed, paraffin embedded mouse tissue? If so, would anyone mind sharing any advice and/or protocols? any help would be greatly appreciated. Graham Brown Garrison Institute on Aging Dept. of Neurology Texas Tech University Health Sciences Center Lubbock, TX From Tony_Reilly <@t> health.qld.gov.au Thu Nov 30 00:56:12 2006 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Thu Nov 30 00:57:37 2006 Subject: [Histonet] Alcian Yellow Message-ID: Hi All For Australian subscribers, does anybody know of a supplier of Alcian Yellow? I have been through a number of catelogues without any success. Thanks in advance for any assistance. regards Tony Reilly Chief Scientist Anatomical Pathology QHPS-Prince Charles Hospital Rode Rd Chermside Q 4032 Australia Ph: 07 3139 4543 Fax: 07 3193 4546 tony_reilly@health.qld.gov.au ***************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/ received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. **************************************************************** From karenadams <@t> comcast.net Thu Nov 30 02:50:15 2006 From: karenadams <@t> comcast.net (karenadams@comcast.net) Date: Thu Nov 30 02:50:25 2006 Subject: [Histonet] help w/ rapid pap stain Message-ID: <113020060850.14540.456E9B47000DF1D0000038CC22007636929C030E0B0E020A9D0E05@comcast.net> can anyone send protocol on rapid pas or ultrafast rapid pap...or is there a particular kit that are favorites out there.....we are taking over all non gyn's for the hospital....also, or Pathologists wants us to stain small batches together w/ blank slides in between to prevent cross contamination....is this protocol, and will this suffice w/ CAP? Any input would be appreciated! Karen -- Karen Adams Pathology Laboratories West 9303 Park West Blvd Knoxville, TN 37923 (865) 690-2111 From sonya.martin <@t> soton.ac.uk Thu Nov 30 04:27:57 2006 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Thu Nov 30 04:28:10 2006 Subject: [Histonet] Cell permeabilization and cell surface staining Message-ID: <71437982F5B13A4D9A5B2669BDB89EE4023E3497@ISS-CL-EX-V1.soton.ac.uk> Hi all, I have been staining BMDC's to look at proteins which are present on the cell surface and in the cytoplasm. I fix the cells with 4% paraformaldehyde in PBS, 20min on ice and permeabilize with 0.2% Triton X100 in PBS for 20min at room temp. My two problems are: 1) When I dont permeabilize the cells I can still detect internal antigen with my primary antibody (cells are fixed 4% PFA, washed PBS and blocked with 2% BSA before incubation with antibody) 2) When I permeabilize the cells I get a decrease in the cell surface staining Any suggestions greatly appreciated. BW Sonya From osullivan <@t> mpih-frankfurt.mpg.de Thu Nov 30 04:51:05 2006 From: osullivan <@t> mpih-frankfurt.mpg.de (Gregory A. O'Sullivan) Date: Thu Nov 30 04:51:18 2006 Subject: [Histonet] Anti GFP on cryosections References: <1164796806.456d6386dedc5@webmail.technion.ac.il> Message-ID: <007b01c7146d$70688fc0$d205058d@MPIHFrankfurt.mpg.de> Dear Adi It is not clear if you are using transgenic mice expressing GFP and if you are able to see GFP fluorescence from these frozen cryosections. However, in our experience with Transgenic mouse lines expressing GFP that have been frozen prior to crosection preparation, is that we loose GFP fluorescence and are unable to detect GFP with a primary antibody. However, if we fix the tissue in 4% PFA and subsequently cryoprotect with 30% Sucrose prior to the preparation of crysections, we can see nice GFP fluorescence. So if you just want see whether GFP is being expressed, then I would try this first. The primary antibody we used is the one from Clontech. Regards, Greg ----- Original Message ----- From: "Adi Sabag" To: "Histonet" Sent: Wednesday, November 29, 2006 11:40 AM Subject: [Histonet] Anti GFP on cryosections Hi All, Does anyone have an experience with anti-GFP antibody of MBL on cryosections? Thanks- Adi -- Adi Sabag Tumor Progression and Angiogenesis laboratory Medicine Faculty, Technion Haifa, Israel. 972-4-8295425 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jonathan.Arzt <@t> ARS.USDA.GOV Thu Nov 30 07:06:04 2006 From: Jonathan.Arzt <@t> ARS.USDA.GOV (Arzt, Jonathan) Date: Thu Nov 30 07:06:45 2006 Subject: [Histonet] In Situ Probe Design Message-ID: <6B4AF69268EFAE4C80A786BA99F7A667B7E074@MD-MAIL-01.ARSNET.ARS.USDA.GOV> Does anyone have experience designing DNA oligos to probe tissues for RNA viruses? I am considering the choices of terminal versus internal nucleotide labeling and biotin versus digoxigenin. (Detection will be via HRP) Also, any thoughts on optimal probe length? Thanks, Jon ******************************************************** J.Arzt DVM, MPVM, DACVP Foreign Animal Disease Research Unit USDA/ARS Plum Island Animal Disease Center Jonathan.Arzt@ARS.USDA.GOV ******************************************************** From jysho <@t> hkusua.hku.hk Thu Nov 30 08:22:48 2006 From: jysho <@t> hkusua.hku.hk (Janice Ho) Date: Thu Nov 30 08:23:11 2006 Subject: [Histonet] Thank you for all your help in rat brain processing Message-ID: <1164896568.456ee93800e43@imp.webmail.hku.hk> Dear all, Thanks for giving me advice for the whole rat brain tissue sectioning. I will modified my protocol and hope that I can have a successful brain tissue sectioning. Thanks again. Janice Ho The University of Hong Kong From debbiekeith <@t> cox.net Thu Nov 30 10:32:42 2006 From: debbiekeith <@t> cox.net (Debbie Keith) Date: Thu Nov 30 10:32:51 2006 Subject: [Histonet] Leica cm1510 problem/question Message-ID: <5.2.0.9.0.20061130090808.0276aa30@pop.central.cox.net> hey! i use a Leica 1510 in a busy Mohs practice... and have been experiencing a problem with advancement/retraction. apparently, this model has something of a design flaw. (the wiring to the advance/retract button is "too long" and is subject to shorts) you can't turn the wheel faster than a really old man walks. if you DO, the chuck doesn't advance at the right micron. in order to return the stupid thing to "normal" you have to completely advance/retract the chuck. it's like re-setting the thing. i'm pretty sure big tissue also causes some advancement-integrity issues. (again, re-set by advance/retraction). in the past i THOUGHT i had fixed it by tightening "this or that"... but after much thought/reflection/self-flagellation... i have realized that the tightening of "this or that" CAUSED me to adjust the chuck advance/retraction... therefore, re-setting the stupid thing. so, the tightening wasn't the fix it was WHAT the tightening CAUSED that fixed it. (can you feel my eyes rolling??) i don't know WHY wheel rotation speed would effect advancement. if it were electrical (the recently replaced wire) then the mechanical bits shouldn't effect it. i posed the conundrum to the local aerospace engineer (my hubby)... and he said that it SOUNDS like there are "gears" that interconnect that are "wearing". he said gears have a built-in "lash". (meaning gears don't fit perfectly). over time the lash gets greater and greater... and something like increased speed would make the lash more pronounced so, where two gears USED be slightly and meeting on one side... they are now meeting at a totally unlikely spot and it throws off performance and requires a re-tare of sorts. if you didn't flippin' glaze over during that last paragraph... it sorta makes sense. :) so i have TWO questions... one, is anyone else having this problem or have had it successfully diagnosed? and TWO... what cryostat would you recommend for heavy use? i've used the leica 1800 and 1850 and they seem to be the lab work-horse. the 1510... is more like the lab miniature pony (with which you can pull a small cart for fun... but not far). :) thanks in advance! :) -- No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.1.409 / Virus Database: 268.15.0/557 - Release Date: 11/29/2006 From Janet.Bonner <@t> FLHOSP.ORG Thu Nov 30 10:57:33 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Thu Nov 30 11:00:14 2006 Subject: [Histonet] Leica cm1510 problem/question References: <5.2.0.9.0.20061130090808.0276aa30@pop.central.cox.net> Message-ID: <5F31F38C96781A4FBE3196EBC22D478004A6C9@fhosxchmb006.ADVENTISTCORP.NET> We are using the Leica CM1900. We're a big Hospital and (literally) have frozen sections all day long! The only service is really the PM and we have had these instruments eight years - at least! Maybe you could trade yours in? @:) Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Debbie Keith Sent: Thu 11/30/2006 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica cm1510 problem/question hey! i use a Leica 1510 in a busy Mohs practice... and have been experiencing a problem with advancement/retraction. apparently, this model has something of a design flaw. (the wiring to the advance/retract button is "too long" and is subject to shorts) you can't turn the wheel faster than a really old man walks. if you DO, the chuck doesn't advance at the right micron. in order to return the stupid thing to "normal" you have to completely advance/retract the chuck. it's like re-setting the thing. i'm pretty sure big tissue also causes some advancement-integrity issues. (again, re-set by advance/retraction). in the past i THOUGHT i had fixed it by tightening "this or that"... but after much thought/reflection/self-flagellation... i have realized that the tightening of "this or that" CAUSED me to adjust the chuck advance/retraction... therefore, re-setting the stupid thing. so, the tightening wasn't the fix it was WHAT the tightening CAUSED that fixed it. (can you feel my eyes rolling??) i don't know WHY wheel rotation speed would effect advancement. if it were electrical (the recently replaced wire) then the mechanical bits shouldn't effect it. i posed the conundrum to the local aerospace engineer (my hubby)... and he said that it SOUNDS like there are "gears" that interconnect that are "wearing". he said gears have a built-in "lash". (meaning gears don't fit perfectly). over time the lash gets greater and greater... and something like increased speed would make the lash more pronounced so, where two gears USED be slightly and meeting on one side... they are now meeting at a totally unlikely spot and it throws off performance and requires a re-tare of sorts. if you didn't flippin' glaze over during that last paragraph... it sorta makes sense. :) so i have TWO questions... one, is anyone else having this problem or have had it successfully diagnosed? and TWO... what cryostat would you recommend for heavy use? i've used the leica 1800 and 1850 and they seem to be the lab work-horse. the 1510... is more like the lab miniature pony (with which you can pull a small cart for fun... but not far). :) thanks in advance! :) -- No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.1.409 / Virus Database: 268.15.0/557 - Release Date: 11/29/2006 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From Jessica <@t> medstaffservices.com Thu Nov 30 13:53:04 2006 From: Jessica <@t> medstaffservices.com (Jessica Hirsch) Date: Thu Nov 30 13:53:18 2006 Subject: [Histonet] Histology Employment Opportunities Message-ID: CURRENT EMPLOYMENT OPPROTUNITIES ( HISTOLOGY, IMMUNOHISTOCHEMISTRY & PATHOLOGY ) I. Clarksburg, Maryland (Large Nationwide Clinical Labratory (division of BioReference)) 1. Histology Supervisor: Hours: Monday - Friday 12 midnight - 8:30am Pay: $28-30/hr Benefits: Medical, Dental, 401k 2. Histology Technologist Hours: Monday - Friday 12 midnight - 8:30am Pay: $25/hr Benefits: Medical, Dental, 401k 3. Grossers Hours: Monday - Friday 7pm-11:30pm Pay: $30/hr II. Elmwood Park, New Jersey ( Nationwide Clinical Laboratory) 1. Histology Technologist ( 2 positions available, immediate start ) Hours: Monday - Friday, 9:00pm - 5:30am Pay: Negotiable Benefits: Medical, Dental, 401k 2. ImmunoHistoChemistry Supervisor Hours: Monday - Friday, 7am or 8am - 4pm or 5pm Pay: Negotiable ~$70,000+ Benefits: Medical, Dental, 401k III. New York City, New York ( large world renowned Cancer Hospital ) 1. Pathologist Assistant Hours: Monday - Friday, Day shift, Full-Time Pay: $60k - $80k based on experience Benefits: Major Medical, Dental, Vision, 401k, etc. 2. Histology Technologist Hours: Monday - Friday (shift to be announced) Pay: negotiable Benefits: Medical, Dental, 401k For immediate consideration for any position: Send resumes to Jessica@medstaffservices.com OR fax to 732.238.2152 For questions call: Jessica Hirsch at 732.238.6050 ext.58 Jessica D. Hirsch Staffing Specialist Medical Staffing Services, Inc. 557 Cranbury Road East Brunswick, NJ 08816 P: (732) 238-6050 x58 F: (732) 238-2152 Jessica@medstaffservices.com http://www.medstaffservices.com From Charles.Embrey <@t> carle.com Thu Nov 30 14:08:03 2006 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Thu Nov 30 14:08:21 2006 Subject: [Histonet] Histology Employment Opportunities Message-ID: <44780C571F28624DBB446DE55C4D733A1FE484@EXCHANGEBE1.carle.com> Must we be subjected to this same e-mail everyday? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica Hirsch Sent: Thursday, November 30, 2006 1:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology Employment Opportunities CURRENT EMPLOYMENT OPPROTUNITIES ( HISTOLOGY, IMMUNOHISTOCHEMISTRY & PATHOLOGY ) I. Clarksburg, Maryland (Large Nationwide Clinical Labratory (division of BioReference)) 1. Histology Supervisor: Hours: Monday - Friday 12 midnight - 8:30am Pay: $28-30/hr Benefits: Medical, Dental, 401k 2. Histology Technologist Hours: Monday - Friday 12 midnight - 8:30am Pay: $25/hr Benefits: Medical, Dental, 401k 3. Grossers Hours: Monday - Friday 7pm-11:30pm Pay: $30/hr II. Elmwood Park, New Jersey ( Nationwide Clinical Laboratory) 1. Histology Technologist ( 2 positions available, immediate start ) Hours: Monday - Friday, 9:00pm - 5:30am Pay: Negotiable Benefits: Medical, Dental, 401k 2. ImmunoHistoChemistry Supervisor Hours: Monday - Friday, 7am or 8am - 4pm or 5pm Pay: Negotiable ~$70,000+ Benefits: Medical, Dental, 401k III. New York City, New York ( large world renowned Cancer Hospital ) 1. Pathologist Assistant Hours: Monday - Friday, Day shift, Full-Time Pay: $60k - $80k based on experience Benefits: Major Medical, Dental, Vision, 401k, etc. 2. Histology Technologist Hours: Monday - Friday (shift to be announced) Pay: negotiable Benefits: Medical, Dental, 401k For immediate consideration for any position: Send resumes to Jessica@medstaffservices.com OR fax to 732.238.2152 For questions call: Jessica Hirsch at 732.238.6050 ext.58 Jessica D. Hirsch Staffing Specialist Medical Staffing Services, Inc. 557 Cranbury Road East Brunswick, NJ 08816 P: (732) 238-6050 x58 F: (732) 238-2152 Jessica@medstaffservices.com http://www.medstaffservices.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From debbiekeith <@t> cox.net Thu Nov 30 14:48:53 2006 From: debbiekeith <@t> cox.net (Debbie Keith) Date: Thu Nov 30 14:49:04 2006 Subject: [Histonet] non-pathologist grossing procedure In-Reply-To: <70EEF3D43B3C164C94037D811B2BE193E12661@VHAV20MSGA3.v20.med .va.gov> References: Message-ID: <5.2.0.9.0.20061130134614.04bc8ea0@pop.central.cox.net> Clia 88 stipulates one must have been trained before 1995 if they do not have any college credit. After 1995, you must have at least 60 semester hours, with courses in chemistry, biology, anatomy. You do not have to be certified, but your procedures must include the types of specimens one may gross under the direct supervision of a pathologist as well as documentation of at least annual performance. i KNOW this... because i tracked down the pathologist i worked for in the early 90's to get documentation. THAT was like finding a missing FUGITIVE! ;) Deb -- No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.1.409 / Virus Database: 268.15.2/560 - Release Date: 11/30/2006 From SDrew <@t> uwhealth.org Thu Nov 30 14:54:28 2006 From: SDrew <@t> uwhealth.org (Drew Sally A.) Date: Thu Nov 30 14:54:38 2006 Subject: [Histonet] Info on lab microwaves Message-ID: Our lab is using an older EMS Lab Microwave to do histochemical special stains and it needs to be replaced. What brand/model lab microwave ( not household) are others using for histochemical staining? Any positives or negatives to pass along? Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 (608)265-6596 From Charles.Embrey <@t> carle.com Thu Nov 30 15:51:56 2006 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Thu Nov 30 15:52:07 2006 Subject: [Histonet] Histology Employment Opportunities Message-ID: <44780C571F28624DBB446DE55C4D733A1FE485@EXCHANGEBE1.carle.com> I have been with Histonet for about 8 years now and the number of messages I get each day has grown by leaps and bounds. Early on, the delete key would take care of threads and items I wasn't interested in, but now as things have grown I can understand why the founders of the histonet asked in the very beginning that vendors not use the histonet to advertise. The problem is that there are other lab headhunters on histonet and are courteous enough to keep their postings to once every other week or so. If all the others felt as you do the messages would increase greatly. Sorry, I just can't believe that your business is more important, and thus exempt from histonet policies, than the other hundred or so businesses that monitor histonet. Charles Embrey, PA(ASCP) -----Original Message----- From: Jessica Hirsch [mailto:Jessica@medstaffservices.com] Sent: Thursday, November 30, 2006 2:11 PM To: Charles.Embrey Subject: RE: [Histonet] Histology Employment Opportunities Charles- Sorry I keep updating it, there has been changes made to pay rates, shifts, etc. I want to keep you updated as best as I can. Plus, I have had many responses from people looking in these areas. So, the answer is yes, as long as people are responding and the positions are open, I will continue to update the list. Please disregard it if you are not interested, but there are plenty of others who are. I am sorry if this is an annoyance to you. Jessica D. Hirsch Staffing Specialist Medical Staffing Services, Inc. 557 Cranbury Road East Brunswick, NJ 08816 P: (732) 238-6050 x58 F: (732) 238-2152 Jessica@medstaffservices.com http://www.medstaffservices.com -----Original Message----- From: Charles.Embrey [mailto:Charles.Embrey@carle.com] Sent: Thursday, November 30, 2006 3:08 PM To: Jessica Hirsch; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Histology Employment Opportunities Must we be subjected to this same e-mail everyday? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica Hirsch Sent: Thursday, November 30, 2006 1:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology Employment Opportunities CURRENT EMPLOYMENT OPPROTUNITIES ( HISTOLOGY, IMMUNOHISTOCHEMISTRY & PATHOLOGY ) I. Clarksburg, Maryland (Large Nationwide Clinical Labratory (division of BioReference)) 1. Histology Supervisor: Hours: Monday - Friday 12 midnight - 8:30am Pay: $28-30/hr Benefits: Medical, Dental, 401k 2. Histology Technologist Hours: Monday - Friday 12 midnight - 8:30am Pay: $25/hr Benefits: Medical, Dental, 401k 3. Grossers Hours: Monday - Friday 7pm-11:30pm Pay: $30/hr II. Elmwood Park, New Jersey ( Nationwide Clinical Laboratory) 1. Histology Technologist ( 2 positions available, immediate start ) Hours: Monday - Friday, 9:00pm - 5:30am Pay: Negotiable Benefits: Medical, Dental, 401k 2. ImmunoHistoChemistry Supervisor Hours: Monday - Friday, 7am or 8am - 4pm or 5pm Pay: Negotiable ~$70,000+ Benefits: Medical, Dental, 401k III. New York City, New York ( large world renowned Cancer Hospital ) 1. Pathologist Assistant Hours: Monday - Friday, Day shift, Full-Time Pay: $60k - $80k based on experience Benefits: Major Medical, Dental, Vision, 401k, etc. 2. Histology Technologist Hours: Monday - Friday (shift to be announced) Pay: negotiable Benefits: Medical, Dental, 401k For immediate consideration for any position: Send resumes to Jessica@medstaffservices.com OR fax to 732.238.2152 For questions call: Jessica Hirsch at 732.238.6050 ext.58 Jessica D. Hirsch Staffing Specialist Medical Staffing Services, Inc. 557 Cranbury Road East Brunswick, NJ 08816 P: (732) 238-6050 x58 F: (732) 238-2152 Jessica@medstaffservices.com http://www.medstaffservices.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Thu Nov 30 16:15:57 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Nov 30 16:16:11 2006 Subject: [Histonet] FrozFix for frozen sections Message-ID: <6.0.0.22.1.20061130150835.01b4c228@gemini.msu.montana.edu> Has anyone tried FrozFix from Newcomers? On what species, tissues and for what markers? Always interested when something new comes on the market that could improve morphology along with immunostaining on frozen sections. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From RSRICHMOND <@t> aol.com Thu Nov 30 19:49:29 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Thu Nov 30 19:49:43 2006 Subject: [Histonet] Re: Fixation and Immunos Message-ID: Kimberly Secrest HTL, QIHC at West Virginia University Hospital asks about >> using alcohol/formalin combination fixatives [such as] Penn-fix has been brought up by pathologists and [pathologist's assistants], but there is also concern about the effects the fixative may have on immunostaining.<< You'd be using a fixative like that mostly to find small lymph nodes in colon resection specimens and some other cancer resection specimens (not including breast, at least for my personal preference). You wouldn't being doing immunostains on such material anyway. If you need to do them in the future, you'll just have to try and see. I prefer making up my own fixative (such as Davidson's fixative, which is three parts water, three parts alcohol, two parts strong [37%] formalin, one part glacial acetic acid) or using a commercial product called O-Fix. Bob Richmond Samurai Pathologist Knoxville TN From Iris.Yeung <@t> vch.ca Thu Nov 30 15:04:11 2006 From: Iris.Yeung <@t> vch.ca (Yeung, Iris [VA]) Date: Tue Dec 5 10:51:56 2006 Subject: [Histonet] H&E slides fading in a few days Message-ID: <00F6A285218A0643975FE0E4F22930A40152015C@vchexmb5.vch.ca> Hi, We have problem with the slides fading in a relatively short time. Our water supply is high in chlorine. We use Gill's III and alcoholic eosin. Coverslip in Entellin(?spelling) and glass coverslip. Seems to fade in small bx like prostatic needle bx than larger blocks. Any suggestion why it happens?? Yeung From mobine <@t> MIT.EDU Thu Nov 30 16:19:23 2006 From: mobine <@t> MIT.EDU (Hector Mobine) Date: Tue Dec 5 10:51:58 2006 Subject: [Histonet] Dilated Cardiomyopathy Message-ID: <456F58EB.4050009@mit.edu> I am in the process of collecting and imaging heart sections from rats in order to visualize the markers of dilated cardiomyopathy. Unfortunately I have been unable to find a suitable outline of what those markers would be and what to look for. Does anyone know of a good guide/atlas/outline that would be of help. Thanks in advance, Hector