From rachael_emerson <@t> urmc.rochester.edu Mon May 1 06:47:12 2006 From: rachael_emerson <@t> urmc.rochester.edu (Rachael Emerson) Date: Mon May 1 06:47:43 2006 Subject: [Histonet] Liver sections Message-ID: Hello. I need some advice about cutting adult mouse livers. The livers are fixed in paraformaldehyde overnight, rinsed, stored in 70% EtOH, and then processed into paraffin. I usually cut at about 4um, but my sections look like they are thicker...maybe 10um. Everything else I cut looks fine, it?s just the liver. Usually I face the block and let it sit on ice for 1-2 hours before cutting. Sometimes if I am having trouble with shredding, I will let it sit overnight on ice at 4?C. Any advice or suggestions on my thickness problem would be greatly appreciated. Thank you Rachael -- Rachael L. Emerson Center for Pediatric Biomedical Research University of Rochester Medical Center 575 Elmwood Avenue MRBX 1.11301 Rochester, NY 14642 From gcallis <@t> montana.edu Mon May 1 09:22:52 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon May 1 09:22:55 2006 Subject: [Histonet] microglial marker In-Reply-To: References: Message-ID: <6.0.0.22.1.20060501081921.01b1b210@gemini.msu.montana.edu> F4/80 from Serotec a previous message from Barb Wright Histonet using FFPE murine tissue is: We successfully stain with F4/80 (Serotec #MCAP497) on paraffin embedded mouse tissues. We use spleen as a positive control. 0.4% pepsin at 37C for 10 minutes for antigen retrieval and an overnight at 4C incubation in a 10ug/ml dilution of F4/80. If you want the procedure just let me know. Barb Wright At 05:09 AM 4/29/2006, you wrote: > I am looking for a microglial marker to use on 4% PFA perfused, frozen > &/or vibratome mouse brain sections. I have tried MAC-1 from Serotec > with pretty disappointing results. Any suggestions would be greatly > appreciated. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From gcallis <@t> montana.edu Mon May 1 09:24:56 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon May 1 09:26:15 2006 Subject: [Histonet] Histo Question In-Reply-To: References: Message-ID: <6.0.0.22.1.20060501082337.01b0be40@gemini.msu.montana.edu> What is the purpose of your question? More specifics would help. At 01:00 PM 4/30/2006, you wrote: >What is the most difficult stain to work with and what tissue is it most >often used? Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From gcallis <@t> montana.edu Mon May 1 09:42:08 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon May 1 09:42:11 2006 Subject: [Histonet] Liver sections In-Reply-To: References: Message-ID: <6.0.0.22.1.20060501082930.01b72778@gemini.msu.montana.edu> Rachael, If the animal has not been perfused with the PFA fixative, or the liver reduced in size, I doubt a WHOLE liver will be fixed after overnight PFA immersion. We find adult murine liver dropped into NBF for a day and even a few days is NOT fixed, it still looks bloody inside indicating not fixed. If you "breadslice" the liver, fixation will improve since it surrounds all sides of the sliced tissue. After trimming ,oversoaking is not a good idea with this very homogenous tissue - we trim, soak on block of ice with water on top of ice, (can be 45 min to 1 hour if needed) but recently improved sectioning and morphology by removing the block from ice water soak but putting the block into room temperature or even warm water JUST before sectioning, generally improves sectioning. Check the block face and if the tissue has swelled out of the block, then you can get thicker sections. Make sure you back the block away from blade, then reapproach carefully, you do not want to cut off what you have soaked, but go into the tissue just a bit away from the tissue - you do not want to lose the advantage of the soak. Also, readdress your processing, you may be exposing the liver to solvents i.e alcohols way too long aka overprocessing. This removes not only the free water from tissue spaces (that is what you want) but also the bound water on the protein (you do NOT want this to happen) which turns liver (also spleen) into hard little nuggets that shred during sectioning. Soaking helps, but it is still an annoying problem. Poor infiltration of paraffin can also contribute to shredding. At 05:47 AM 5/1/2006, you wrote: >Hello. I need some advice about cutting adult mouse livers. The livers are >fixed in paraformaldehyde overnight, rinsed, stored in 70% EtOH, and then >processed into paraffin. I usually cut at about 4um, but my sections look >like they are thicker...maybe 10um. Everything else I cut looks fine, it?s >just the liver. Usually I face the block and let it sit on ice for 1-2 >hours before cutting. Sometimes if I am having trouble with shredding, I >will let it sit overnight on ice at 4?C. Any advice or suggestions on my >thickness problem would be greatly appreciated. > >Thank you >Rachael > >-- >Rachael L. Emerson >Center for Pediatric Biomedical Research >University of Rochester Medical Center >575 Elmwood Avenue MRBX 1.11301 >Rochester, NY 14642 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From meligroc <@t> zgi.com Mon May 1 10:34:43 2006 From: meligroc <@t> zgi.com (CRME (Criss Meligro)) Date: Mon May 1 10:34:58 2006 Subject: [Histonet] (no subject) Message-ID: <746E68D5CBB205409B12EC32A61EE401D91A2F@ned.zgi.com> We are in the market for a self decontaminating cryostat, have product information on the Ultra Pro 7500 made by myNeuroLab... Has anyone use this machine? Are there other self decontaminating models available? How is the "radial cutting" feature a plus? Any information will be appreciated! Criss Meligro From jkiernan <@t> uwo.ca Mon May 1 10:49:46 2006 From: jkiernan <@t> uwo.ca (John A. Kiernan) Date: Mon May 1 10:49:40 2006 Subject: [Histonet] Evan's Blue, then H&E? References: Message-ID: <44562E1A.366B4B94@uwo.ca> Fixation and sectioning have been done, since ancient times, with Evans blue and the closely similar dye trypan blue, in nervous tissue. Here are three moderately recent references. Sasaki, S. & Schneider, H. (1976). Supravital diffusion of fluorescent Evans blue in brain and spinal cord tissue. Acta Neuropathologica 36: 363-368. (Fixed by perfusion with Bouin) McDonald, D.M. & Blewett, R.W. (1981). Location and size of carotid body-like organs (paraganglia) revealed in rats by the permeability of blood vessels to Evans blue dye. Journal of Neurocytology 10: 607-643. (Fixed by perfusion with glutaraldehyde) Lees, G.J. (1989). In vivo and in vitro staining of acidopilic neurons as indicative of cell death following kainic acid-induced lesions in rat brain. Acta Neuropathologica 77: 519-524. I'd be surprised if these dyes stayed in place through dehydration, paraffin embedding and H&E. Both are fluorescent when bound to protein (green excitation, red emission), and the fluorescence can be seen when the blue colour is too pale to be visible with ordinary microscopy. Evans blue was one of the first fluorochromes to be used as a retrograde neuroanatomical tracer in the early 1970s but was abandoned when better, brighter dyes were found for the same job. If you can get Evans blue to stay put in paraffin sections, H&E is probably not the best stain to use, because the strong fluorescence of eosin could well overpower the rather feeble fluorescence of Evans blue, and haemalum suppresses any fluorescence at sites that it stains. A delicate contrasting fluorescent nuclear stain such as DAPI (UV excitation, blue emission, when bound by DNA) would give you a better chance of seeing any residual Evans blue. Just a few thoughts; no substitute for trying it out! -- ------------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan[AT]uwo.ca http://publish.uwo.ca/~jkiernan/ http://instruct.uwo.ca/anatomy/530/index.htm _______________________________ Bret Jessee wrote: > > I'm using in vivo perfusion of Evan's Blue to assess damage of vascular endothelium by endovascular devices. Once I have done a gross exam of tissues explanted en bloc, can standard H&E procedures (10% NBF fixation, paraffin embedding, H&E staining) be used without interference from the Evan's Blue? > > Thanks, > > BRET > > C. Bret Jessee, Ph.D. > Director Preclinical Affairs > OmniSonics Medical Technologies, Inc. > 66 Concord Street, Suite A > Wilmington, MA 01887 > TEL: 978-657-9980 x332 > CELL: 978-394-3175 > Notice: This e-mail message and any attachments to it may contain legally privileged confidential information. > If you are not the intended recipient, you must not review, retransmit, convert to hard copy, copy, use or disseminate > this e-mail or any attachments. If you have received this e-mail in error, please immediately notify us by return > e-mail or by telephone at 978-657-9980 and delete this message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brett_connolly <@t> merck.com Mon May 1 10:50:30 2006 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Mon May 1 10:50:54 2006 Subject: [Histonet] human-on-human IHC Message-ID: <355C35514FEAC9458F75947F5270974D67CE6E@usctmx1103.merck.com> H'netter's I need advise on using human antibodies on human tissue. It will be an unlabeled human primary. i.e. what blocking protocol do you recommend...any other specific caveats you can point out? Thanks, Brett Brett M. Connolly, Ph.D. Merck & Co., Inc. MRL, Imaging Research WP-44K PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------------ From Sarah_Mack <@t> urmc.rochester.edu Mon May 1 11:04:13 2006 From: Sarah_Mack <@t> urmc.rochester.edu (Sarah Mack) Date: Mon May 1 11:04:35 2006 Subject: [Histonet] (no subject) In-Reply-To: <746E68D5CBB205409B12EC32A61EE401D91A2F@ned.zgi.com> Message-ID: Check into the Leica CM1850UV. We are currently looking into purchasing this model. Hope this is useful to you. Sarah Mack > From: "CRME \(Criss Meligro\)" > Date: Mon, 1 May 2006 08:34:43 -0700 > To: > Subject: [Histonet] (no subject) > > We are in the market for a self decontaminating cryostat, have product > information on the Ultra Pro 7500 made by myNeuroLab... Has anyone use this > machine? Are there other self decontaminating models available? How is the > "radial cutting" feature a plus? Any information will be appreciated! > Criss Meligro > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From gpbnas <@t> yahoo.es Mon May 1 11:44:35 2006 From: gpbnas <@t> yahoo.es (Guillermo Palao) Date: Mon May 1 11:44:40 2006 Subject: [Histonet] Collagen immunohistochemistry. Enzymatic or heat treatment of frozen sections. Message-ID: <20060501164435.10007.qmail@web26215.mail.ukl.yahoo.com> Hello all, I am working with frozen sections to quantify collagen staining. I am using Anti-Collagen-I Ab from Calbiochem, and they recommend fixing sections after heating or enzymatic treatment with hylaronidase. I have a stock of already acetone-fixed frozen sections where I have tried staining with Anticollagen without success. Before staining I heated fixed sections for 10 min at 42 ?C. I now plan to try hyaluronidase treatment, but I would like to know if anyone has experience staining sections with anti-Collagen once they are already fixed with acetone without previous enzymatic or heat treatment. What is the best way to do it? Is it necessary to cut new tissue and treat it with heat or hyaluronidase before fixation? As always thank to all you in advance, Guillermo --------------------------------- LLama Gratis a cualquier PC del Mundo. Llamadas a fijos y m?viles desde 1 c?ntimo por minuto. http://es.voice.yahoo.com From tpmorken <@t> labvision.com Mon May 1 11:55:49 2006 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Mon May 1 11:55:55 2006 Subject: [Histonet] Expired antibodies...for immunos.. Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA20328D89B@usca0082k08.labvision.apogent.com> Here is some info on this from a vendor standpoint. A vendor supplies antibodies and guarantees a certain lifetime. That lifetime is limited by the expiration date. The vendor has to do stability tests to support the expiry date (required by FDA and CE (European Union)). Various tests are used for stability testing - Some may use accelerated tests ( such as a certain time in an oven at a certain temp is considered one year or two years), or may do real time tests - leave it in the fridge for two years. In any case it is mainly to satisfy the regulatory agencies that the reagent is indeed stable for a given period of time. The time period is up to the vendor. The main concern is liability over time. Since a vendor guarantees a reagent for a given time it means if they are wrong about the stability they have to replace the reagent. If the vendor gave something too far out they may end up replacing a lot of reagent. So it is in the vendors interest to be conservative on these expiry dates. That means the antibody most likely will be good well after the expiry date. So the expiry date is a limit on the liability of the vendor, not an absolute drop-dead date. But it is certainly not arbitrary either. Vendors aren't going to offer unlimited time to get your money back if it does not work. Also vendors would rather offer one set of expiry dates than customizing it for every antibody. Most vendors are too small to support complicated stability testing for every antibody they offer. I'm not sure why CAP and CLIA and CLMA have insisted on absolute recognition of the expiry dates. But my personal opinion is that it is probably because they don't want to be liable for accrediting labs using self-labeled extended expiry dates - a definite lawyer magnent if things go wrong. While I have done this very thing for years, and have seen many labs do it, essentially they don't trust the labs to do the job well. What do do about it? Most vendors probably won't want to extend their liability for the reagents. The alternative is to order lower quantities. If the vendor does not supply in small quantities, ask them to do so. If enough people ask, they will get the message. Vendors want people to be happy with the product, but aren't necessarily going to offer something no one asks for. Tim Morken Lab Vision/Neomarkers Re: [Histonet] Expired antibodies...for immunos.. From: Rene J Buesa ---------------------------------------------------------------------------- ---- Hi all: I want to jump into this thread that has already been a subject of discussion on November of last year. 1- I had never this expriration problem because our IHC volume was large, but is is something a great concern to small laboratories, some of which have opted not to do IHC and send it to a reference lab. 2- The experiration date is set by the manufacturer, but no manufacturer says which studies are behing their dating system. 3- CAP has now stablished a rigid rule about not allowing "expired" Abs to be used BUT the same CAP in thei Laboratory Improvement Programs published in 1998 the following: Tubbs et al. (1998): "Extension of Useful Reagent Shelf Life Beyond Manufacturer's Recommendations" Arch Pathol Lab Med. 122: 1051-52 and concluded: "The data suggest review of the Health Care Financing Administration's ruling on extending the useful reagent shelf life beyond manufacturers recommendations. Similar studies using more inherently quantitative methodology are suggested" (sic.) 4- The next year Balaton et al. published a paper under the title of: "Satisfactory performance of primary antibodies beyond manufacturers' recommended expiration dates" Applied Immunohistochemistry & Molecular Morphology 7(3):221-25 They tested 65 antibodies that had expired 6 to 134 months previously (average of 33 months) and concluded that"....These results indicate that primary antibodies have a shelf life significantly longer than the one specified by the manufacturers...."(sic.). 5- It is worth noting that both papers stress the fact that those expiration dates are manufacturers' recommendations, they are not written on stone. The one now doing the writing on stone is CAP after sponsoring a contradictory study. So, when you decide to flame Joe Nocito, please leave a space for me. Joe's suggestion seems to be very reasonable. Another thing, please use cajun sauce for my roasting. Best wishes Ren? J. Dana Settembre wrote: Hello Kat, JCAHO does not allow anything expired to be used for patients Antibodies cannot be used either, if they are expired. Dana Settembre University Hospital-UMDNJ Newark, NJ >>> 04/27/06 3:23 PM >>> How does JCAHO handle expired antibodies if you have them...and are using them... Is it ok ....or not?? Kat _______________________________________________ ---------------------------------------------------------------------------- ---- << Previous Message | Next Message >> Tim Morken Product Development Lab Vision - Neomarkers Free webhosting for US State Histotechnology Societies: http://www.labvisioncorp.com/demowebsite/index.cfm From JEllin <@t> yumaregional.org Mon May 1 11:59:00 2006 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Mon May 1 11:58:12 2006 Subject: [Histonet] Breast processing and or fatty tissue Message-ID: <29BE166A2CF48D459853F8EC57CD37E8073B32@EXCHANGECLUSTER.yumaregional.local> Hello all, what I am wondering is how people are handleing their breast tisse? We are wanting a protocal for either breast or fatty tissue for a traditional cycle. Also is anyone using a microwave to speed up fixation before they go into the traditional processor. Are there solutions avaiable that can help with the traditional processing. Jesus Ellin Yuma Regional Medical Center From pruegg <@t> ihctech.net Mon May 1 12:39:10 2006 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon May 1 12:39:12 2006 Subject: [Histonet] Collagen immunohistochemistry. Enzymatic or heattreatment of frozen sections. In-Reply-To: <20060501164435.10007.qmail@web26215.mail.ukl.yahoo.com> Message-ID: <200605011739.k41Hd3og007545@chip.viawest.net> The hylaronidase is to unmask the target site for the collagen antibody, the hyalaronic acid region of the collagen epitope needs to be digested away so you can get at the collagen 1 expression. It is not a fixative, it is more like enzyme induced epitope retrieval (eier). I did find it necessary to get good staining for some collagens. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Guillermo Palao Sent: Monday, May 01, 2006 9:45 AM To: Histonet Subject: [Histonet] Collagen immunohistochemistry. Enzymatic or heattreatment of frozen sections. Hello all, I am working with frozen sections to quantify collagen staining. I am using Anti-Collagen-I Ab from Calbiochem, and they recommend fixing sections after heating or enzymatic treatment with hylaronidase. I have a stock of already acetone-fixed frozen sections where I have tried staining with Anticollagen without success. Before staining I heated fixed sections for 10 min at 42 ?C. I now plan to try hyaluronidase treatment, but I would like to know if anyone has experience staining sections with anti-Collagen once they are already fixed with acetone without previous enzymatic or heat treatment. What is the best way to do it? Is it necessary to cut new tissue and treat it with heat or hyaluronidase before fixation? As always thank to all you in advance, Guillermo --------------------------------- LLama Gratis a cualquier PC del Mundo. Llamadas a fijos y m?viles desde 1 c?ntimo por minuto. http://es.voice.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jamie.erickson <@t> abbott.com Mon May 1 12:42:29 2006 From: jamie.erickson <@t> abbott.com (Jamie E Erickson) Date: Mon May 1 12:42:37 2006 Subject: [Histonet] Re:human-on-human IHC In-Reply-To: <200605011710.k41HAWGl026696@vette.abbott.com> Message-ID: Hi Brett, I haven't done exactly what your talking about but this may help. I'm sure your secondary anti-human will be a problem. When I did some crossreactivity studies in various species with a humanized antibody I used a protocol that was a Modification of the method of Tuson, Fung, Hierck and their colleagues (Tuson et al. 1990; Fung et al. 1992; Hierck et al. 1994) that involved mixing the primary and secondary together overnight at 4 degrees C in a tube. the next day I added gamma globulin (human) to bind unbound secondary then this mixture was applied to the section like a primary antibody. I had good results in various species with little background but did not have human tissue to try it out on. It just one way to get around the secondary nonspecific binding. If you would like more information contact me I think I have the protocol. _______________________________ Jamie Erickson Sr. Research Associate Department: DSMP Abbott Bioresearch Center 100 Research Drive Worcester, MA 01605-4341 508-688-3134 FAX: 508-793-4895 e-mail: jamie.erickson@abbott.com From pruegg <@t> ihctech.net Mon May 1 12:44:09 2006 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon May 1 12:44:12 2006 Subject: [Histonet] Expired antibodies...for immunos.. In-Reply-To: <004601c66be1$774d3500$1bb30b43@yourxhtr8hvc4p> Message-ID: <200605011744.k41Hi1og008730@chip.viawest.net> I agree that it is idiotic to not allow the use of expired (perfectly validated) antibodies, but please don't throw your expired antibodies down the drain just because CAP tells you they can not be used any more. We can use those in research and teaching. I will provide a fedx shipping number to anyone willing to send me their expired antibodies. Best regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Saturday, April 29, 2006 4:06 PM To: Bryan Hewlett; KMB1904@aol.com; histonet@pathology.swmed.edu Subject: Re: [Histonet] Expired antibodies...for immunos.. only when it's convenient for the other person. Joe ----- Original Message ----- From: "Bryan Hewlett" To: "Joe Nocito" ; ; Sent: Thursday, April 27, 2006 8:15 PM Subject: Re: [Histonet] Expired antibodies...for immunos.. > Hey Joe, > > I'm with you! > The disposal of a expensive, validated, working reagent, just because > of some mindless arbitrary date rule makes absolutely no sense! > I thought that we were in the era of evidence-based medicine, whatever > happened? > > Bryan Hewlett > Consultant technologist > Anatomic Pathology > Quality Management Program - Laboratory Services Ontario, Canada. > > ----- Original Message ----- > From: "Joe Nocito" > To: ; > Sent: Thursday, April 27, 2006 6:27 PM > Subject: Re: [Histonet] Expired antibodies...for immunos.. > > >> Oh boy, >> I get to get flamed again. >> JCAHO and CAP do not let you use expired reagents, including antibodies. >> therefore, everyone gets to through money down the drain time and >> time again. >> I called CAP and talked to a person who got very defensive when I >> asked him to give the rule that says we can not use expired antibodies. >> He referred me to the U.S. National Register. I'm sorry, I don't have >> that information with right now but I'm sure someone will let me know. >> My beef is, if you run a known positive control with every run, >> you are, in essence, revalidating that antibody. Once that antibody >> doesn't work, replace it with one that has not expired. I know this >> causes a repeat and maybe a delay in reporting the results, but ask >> your pathologists if they would want a one day delay, or throw >> thousands of dollars down the sink. >> When I was supervising the immuno lab at the AFIP, we froze >> concentrated antibodies at -70 C and they still worked up to 10 years >> later. Now, you can't even do that. A big waste, that what it is, a >> big waste. >> Let Flaming Friday begin. >> >> Joe Nocito BS, HT(ASCP)QIHC >> Histology Manager >> Pathology Reference Lab >> San Antonio, TX >> >> ----- Original Message ----- >> From: >> To: >> Sent: Thursday, April 27, 2006 2:23 PM >> Subject: [Histonet] Expired antibodies...for immunos.. >> >> >>> How does JCAHO handle expired antibodies if you have them...and are >>> using them... >>> Is it ok ....or not?? >>> Kat >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >>> -- >>> Internal Virus Database is out-of-date. >>> Checked by AVG Free Edition. >>> Version: 7.1.385 / Virus Database: 268.4.3/317 - Release Date: 4/18/2006 >>> >>> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > Internal Virus Database is out-of-date. > Checked by AVG Free Edition. > Version: 7.1.385 / Virus Database: 268.4.3/317 - Release Date: 4/18/2006 > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpmorken <@t> labvision.com Mon May 1 13:25:19 2006 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Mon May 1 13:25:28 2006 Subject: [Histonet] Freezer sample tracking software Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA20328D8A1@usca0082k08.labvision.apogent.com> Someone was asking about software to track samples in freezers. Here are two: Freezerworks www.dwdev.com Cyrotrax www.llamawerx.com/cryotrax Tim Morken From anh2006 <@t> med.cornell.edu Mon May 1 13:45:33 2006 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Mon May 1 13:44:58 2006 Subject: [Histonet] Re:human-on-human IHC In-Reply-To: References: Message-ID: Hi Jamie and Brett, I can confirm that this is a very good approach and works well for human on human staining, however it is only useful for those antigens which are fairly robustly expressed as some sensitivity of the protocol gets lost when precomplexing. If possible, a much more sensitive way to go is to buy a labeling kit from Molecular Probes and label the antibody - with biotin, FITC etc. You can be as creative as you imagine ... It only takes about 1.5-2 hours (much of which is an incubation step) and makes life infinitely easier. The large number of kits they sell are suitable for all different amounts of starting protein and a wide variety of conjugates. Good luck! Andrea At 1:42 PM -0400 5/1/06, Jamie E Erickson wrote: >Hi Brett, > I haven't done exactly what your talking about but >this may help. I'm sure your secondary anti-human will be a problem. > >When I did some crossreactivity studies in various species with a >humanized antibody I used a protocol that was a Modification of the method >of Tuson, Fung, Hierck and their colleagues (Tuson et al. 1990; Fung et >al. 1992; Hierck et al. 1994) that involved mixing the primary and >secondary together overnight at 4 degrees C in a tube. the next day I >added gamma globulin (human) to bind unbound secondary then this mixture >was applied to the section like a primary antibody. I had good results in >various species with little background but did not have human tissue to >try it out on. It just one way to get around the secondary nonspecific >binding. > >If you would like more information contact me I think I have the protocol. > >_______________________________ >Jamie Erickson >Sr. Research Associate >Department: DSMP >Abbott Bioresearch Center >100 Research Drive >Worcester, MA 01605-4341 >508-688-3134 >FAX: 508-793-4895 >e-mail: jamie.erickson@abbott.com -- From ploykasek <@t> phenopath.com Mon May 1 14:24:37 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Mon May 1 14:24:59 2006 Subject: [Histonet] Job opening Message-ID: See below for a job announcement for PhenoPath Laboratories in Seattle, WA (no, it doesn't rain ALL the time). I look forward to the responses. Patti Loykasek IMMUNOHISTOCHEMISTRY TECHNOLOGIST PhenoPath Laboratories, a pathology reference laboratory, has an opportunity in the clinical immunohistochemistry division for a full-time technologist, day shift. We are in a state-of-the-art facility located on the scenic Ship Canal in the eclectic Fremont neighborhood of Seattle, WA. Job Description: Responsibilities may include performing immunohistochemistry, immunofluorescence, molecular testing on patient samples and tissue sectioning (paraffin and frozen). Participation in the development of new tests or technologies is included. Quality control, safety, and preventive maintenance procedures and documentation are required elements of this position as well. Required Skills/Experience: Strong preference given to ASCP certified (or certification-eligible) laboratory techs. Trained laboratory techs of any discipline are encouraged to apply (histotech, med tech, cytotech?..). PhenoPath Laboratories is committed to hiring the best person for the job. PhenoPath Laboratories is a national specialty pathology laboratory committed to providing outstanding clinical and research services. We offer an excellent compensation and benefits package, including 401k. To apply, please e-mail or fax completed application for employment and resume to: Paul Moore, Assistant to the Director of Human Resources PhenoPath Laboratories 551 N. 34th St., Suite 100 Seattle, WA 98103 Phone: 206 374-9000 Fax: 206 374-9009 E-mail: pmoore@phenopath.com ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From gpbnas <@t> yahoo.es Mon May 1 14:49:45 2006 From: gpbnas <@t> yahoo.es (Guillermo Palao) Date: Mon May 1 14:49:53 2006 Subject: [Histonet] Collagen immunohistochemistry. Enzymatic or heattreatment of frozen sections. In-Reply-To: <200605011739.k41Hd3og007545@chip.viawest.net> Message-ID: <20060501194945.33755.qmail@web26205.mail.ukl.yahoo.com> Thanks, but what I would like to know is if you have to use the enzyme in unfixed sections as recommended by manufacturer or in samples already fixed in e.g. acetone. Usually we cut frozen tissue and fix sections in acetone. We then store them at -20 ?C util needed. In fixed samples, is it possible to expose the antigenic site in collagen molecule that is recognized by the Ab? If you have to treat samples with hyaluronidase before fixing the tissue, then I would not be able to use the stored sections, and we would have to change our procedures so to enzymatically treat samples right after cutting tissue blocks, before fixing and storing them. Best regards, Guillermo Patsy Ruegg escribi?: The hylaronidase is to unmask the target site for the collagen antibody, the hyalaronic acid region of the collagen epitope needs to be digested away so you can get at the collagen 1 expression. It is not a fixative, it is more like enzyme induced epitope retrieval (eier). I did find it necessary to get good staining for some collagens. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Guillermo Palao Sent: Monday, May 01, 2006 9:45 AM To: Histonet Subject: [Histonet] Collagen immunohistochemistry. Enzymatic or heattreatment of frozen sections. Hello all, I am working with frozen sections to quantify collagen staining. I am using Anti-Collagen-I Ab from Calbiochem, and they recommend fixing sections after heating or enzymatic treatment with hylaronidase. I have a stock of already acetone-fixed frozen sections where I have tried staining with Anticollagen without success. Before staining I heated fixed sections for 10 min at 42 ?C. I now plan to try hyaluronidase treatment, but I would like to know if anyone has experience staining sections with anti-Collagen once they are already fixed with acetone without previous enzymatic or heat treatment. What is the best way to do it? Is it necessary to cut new tissue and treat it with heat or hyaluronidase before fixation? As always thank to all you in advance, Guillermo --------------------------------- LLama Gratis a cualquier PC del Mundo. Llamadas a fijos y m?viles desde 1 c?ntimo por minuto. http://es.voice.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- LLama Gratis a cualquier PC del Mundo. Llamadas a fijos y m?viles desde 1 c?ntimo por minuto. http://es.voice.yahoo.com From omnivore98 <@t> yahoo.com Mon May 1 15:34:45 2006 From: omnivore98 <@t> yahoo.com (Heather Renko) Date: Mon May 1 15:34:49 2006 Subject: [Histonet] cyrostat Message-ID: <20060501203445.15142.qmail@web31310.mail.mud.yahoo.com> Leica has a really nice cryostat with an UV light that seems to be really nice. Its a good company with a good reputation so servicing would be reliable too. Heather Renko --------------------------------- Blab-away for as little as 1?/min. Make PC-to-Phone Calls using Yahoo! Messenger with Voice. From LRaff <@t> lab.uropartners.com Mon May 1 16:46:35 2006 From: LRaff <@t> lab.uropartners.com (Lester Raff) Date: Mon May 1 16:46:41 2006 Subject: [Histonet] Part time Position in Chicago suburbs Message-ID: <5DA1CA5D0B98A84985B545A24423B822A845@UPLAB01.uplab.local> Hello All: Our private lab in west suburban Chicago is looking for a part-time histotech. If there is anyone in our vicinity who is looking for a few hours a week, please give me a call. Thanks, Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory ph: 708-486-0076 fax: 708-486-0080 From tpmorken <@t> labvision.com Mon May 1 17:09:49 2006 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Mon May 1 17:10:13 2006 Subject: [Histonet] California Society for Histotechnolgy meeting May 18-21 Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA20328D8AA@usca0082k08.labvision.apogent.com> California Society for Histotechnology Thirtieth Annual Meeting MAY 18-21, 2006 COSTA MESA HILTON HOTEL COSTA MESA, CALIFORNIA Information about program and registration at: http://www.californiahistology.org/index.cfm Tim Morken From c.m.vanderloos <@t> amc.uva.nl Tue May 2 02:54:01 2006 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Tue May 2 02:54:19 2006 Subject: [Histonet] RE: human-on-human IHC Message-ID: <250e0124ece3.24ece3250e01@amc.uva.nl> Dear Brett, A couple of days ago I wrote here about the Zenon rabbit-on-rabbit kit. Well, there is also a human-on-human kit available from this vendor (Molecular Probes/Invitrogen). It is based on a labeled (some Alexa-fluorochromes, biotin, HRP) anti-human IgG Fab-fragment used for the in vitro labeling of the humanized primary. Basically, the technology behind is identical with the Dako ARKit. Good luck, Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Mon, 1 May 2006 11:50:30 -0400 From: "Connolly, Brett M" Subject: [Histonet] human-on-human IHC To: histonet@lists.utsouthwestern.edu H'netter's I need advise on using human antibodies on human tissue. It will be an unlabeled human primary. i.e. what blocking protocol do you recommend...any other specific caveats you can point out? Thanks, Brett Brett M. Connolly, Ph.D. Merck & Co., Inc. MRL, Imaging Research WP-44K PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com From sohail_e <@t> yahoo.com Tue May 2 03:35:09 2006 From: sohail_e <@t> yahoo.com (sohail ejaz) Date: Tue May 2 03:35:15 2006 Subject: [Histonet] Protocol for whole mount staining of retina Message-ID: <20060502083509.25141.qmail@web30614.mail.mud.yahoo.com> Dear Friends I am looking for a good protocol rejarding "Whole mount staining of mouse retina". I am interested in screening the effects of different chemicals on retinal vascuature using whole mount staining with Vwf and SMA. Please help me in finding a good protocol in this regard. Thanks Dr. Ejaz --------------------------------- How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. From louise.renton <@t> gmail.com Tue May 2 04:53:23 2006 From: louise.renton <@t> gmail.com (louise renton) Date: Tue May 2 05:00:25 2006 Subject: [Histonet] small metal containers - slightly OT Message-ID: Hi all, First, thanks to all who helped with my immuno question last week. The neurons are slightly less fuzzy now, and I think I have a better idea of what I'm doing. Secondly, my husband has asked me if I know of any thing that might be suitable to store sampling filters in ( these are 3cm diameter filters like millipore ones that are used to collect dust samples by vacuum from dusty environments so that the sample can be ultimately weighed and the duct conc. measured) The ideal container would be non-static, the right size and of a relatively cheap material (he suggested brass, but I think thats OTT) Any ideas - esp from vendors ????? -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "Does a conceited cowboy, only ride on pompous grass?. From mrcraigwbarlow <@t> googlemail.com Tue May 2 05:22:24 2006 From: mrcraigwbarlow <@t> googlemail.com (CRAIG BARLOW) Date: Tue May 2 05:22:29 2006 Subject: [Histonet] Melanin / melanocytes staining method tintorial or IHC Message-ID: <22482b560605020322x259aae90v93914a1e38ac24b7@mail.gmail.com> Dear Histoneters, I have been asked to find out as much as possible about melanin and melanocytes in human skin samples. I would like to detect and possibly quantify racial differences (i.e. skin colour) between skin samples. I know about a masson fontana, schmorl's and a DOPA Oxidase. Does anybody know of any antibodies or other stains that will allow me to do this. Kind Regards Craig Barlow Senior Research Associate From DDDeltour <@t> mar.med.navy.mil Tue May 2 06:25:45 2006 From: DDDeltour <@t> mar.med.navy.mil (Deltour, Douglas D. (HM2)) Date: Tue May 2 06:26:25 2006 Subject: [Histonet] Need Feedback: Milestone Rapid Tissue Processor Message-ID: <3F500F8B416C554EBB21FF16642F72E959CE6D@marxchg03.mar.med.navy.mil> Is anyone currently using or used the Milestone RHS-1 or PATHOS rapid processor? Any feedback would be appreciated. Thanks! DOUGLAS D. DELTOUR HT(ASCP) HISTOLOGY TECHNICIAN NMC PORTSMOUTH VA (757)953-1525 From Bauer.Karen <@t> mayo.edu Tue May 2 09:50:37 2006 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen) Date: Tue May 2 09:51:03 2006 Subject: [Histonet] LEAN in the Histology Lab Message-ID: Hi to all, I'm interested in attending a class or seminar on designing a Histology lab using LEAN principles. I realize that there is a workshop at the NSH conference in Phoenix, but I was wondering if there was one a little closer to home. I'm also interested in visiting any labs that are using the LEAN principles and are within driving distance from Eau Claire, WI. Does anyone have any suggestions? Thanks, Karen L. Bauer HT(ASCP) Histology Supervisor Department of Pathology Luther Hospital Eau Claire, WI 715-838-3205 ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. From ploykasek <@t> phenopath.com Tue May 2 11:23:51 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Tue May 2 11:24:16 2006 Subject: [Histonet] Autostainer tubes Message-ID: HI all. Just wondering if anyone knows of a source for the 12ml plastic reagent tubes that are used on the Dako autostainer in the reagent rack. Thanks for any help. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From Heather.Nymeyer <@t> interiorhealth.ca Tue May 2 12:03:23 2006 From: Heather.Nymeyer <@t> interiorhealth.ca (Nymeyer, Heather) Date: Tue May 2 12:03:29 2006 Subject: [Histonet] CONTROL BLOCKS NEEDED Message-ID: <463911DE3F9F8D4AA3EC67632386BC62D1509E@dc1serv78.interiorhealth.ca> We are in need of the following control blocks - Leprosy bacilli - Spirochete Your assistance is appreciated and thank you in advance. Heather D. Nymeyer, RT, CEBT Charge Technologist, Anatomic Pathology Royal Inland Hospital, Kamloops, BC. 250-314-2664 From pruegg <@t> ihctech.net Tue May 2 11:52:50 2006 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue May 2 12:30:51 2006 Subject: [Histonet] Large sections Message-ID: <200605021652.k42Gqoog014137@chip.viawest.net> Can anyone suggest a resource for getting frozen sections of Elk Larynx? One of my clients asks: "I study elk sound production. As one question we investigate the anatomical structure of the larynx. For this purpose I need cross sections of several larynges (approx. 15 larynges). Cross sections at three positions are necessary for each larynx,. The larynges are most likely calcified at varying degrees. I have stored the larynges frozen in saline solution." Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From ploykasek <@t> phenopath.com Tue May 2 12:31:31 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Tue May 2 12:31:57 2006 Subject: [Histonet] Autostainer tubes Message-ID: Opps - didn't put in there a supplier other than Dako. We had a backorder problem with Dako on these. Actually turned out to be a glitch in their system, so all is well. Thanks for all of the good info, though. Histotechs are a nice bunch of people! Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From carrie <@t> nsh.org Tue May 2 12:37:13 2006 From: carrie <@t> nsh.org (Carrie Diamond) Date: Tue May 2 12:37:19 2006 Subject: [Histonet] NSH Active Members Message-ID: NSH Active Members: Earlier this week you received a ballot and candidate information for the NSH 2006 Election. Unfortunately, an error occurred at the mail house. The mailing lists were incorrectly matched to the wrong region director information. It lists the incorrect region for which you are eligible to vote. We apologize for this mistake and have mailed a corrected ballot indicating the region director who is running for the position within your region. The ballot has been printed on cream colored paper and will be the only ballot counted from your region. We do hope you will return your ballot at your earliest convenience to the Nominations-Election Committee. Carrie Diamond Executive Director National Society for Histotechnology 4201 Northview Drive, Suite 502 Bowie, MD 20716 P: 301-262-6221 E: carrie@nsh.org Join us for the 32nd Annual Symposium Convention Phoenix, AZ September 8-13, 2006 Registration Details will be available in March From NSEARCY <@t> swmail.sw.org Tue May 2 12:49:01 2006 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Tue May 2 12:49:21 2006 Subject: [Histonet] Question Message-ID: Anyone know of an inexpensive tool for cutting "fresh" 1 mm sections? Researcher wants to know. Thanks From vbaker60 <@t> yahoo.com Tue May 2 13:03:59 2006 From: vbaker60 <@t> yahoo.com (Victoria Baker) Date: Tue May 2 13:04:07 2006 Subject: [Histonet] Question In-Reply-To: Message-ID: <20060502180359.42479.qmail@web52513.mail.yahoo.com> The only thing I can think of is what literally looks like an "egg slicer" I think we had ordered it from MOPEC or one of the other surgical/autopsy companies some 6 years ago. It's a metal egg shaped base, and using hi-profile microtome blades you could slice fresh soft tissue (like brain)in thin even pieces. I haven't seen one in sometime, but maybe someone who reads my e-mail will know where they can be purchased these days. If I remember correctly back then it cost about $100. Good luck Vikki --- Nita Searcy wrote: > Anyone know of an inexpensive tool for cutting > "fresh" 1 mm sections? > Researcher wants to know. > > Thanks > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From gcallis <@t> montana.edu Tue May 2 13:31:46 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue May 2 13:32:01 2006 Subject: [Histonet] Large sections In-Reply-To: <200605021652.k42Gqoog014137@chip.viawest.net> References: <200605021652.k42Gqoog014137@chip.viawest.net> Message-ID: <6.0.0.22.1.20060502122207.01b50218@gemini.msu.montana.edu> I don't think the larynx will be calcified, but it will contain both hyaline and elastic cartilage which could be pretty tough to cryosection. The Instrumedics should be able to do the job with a heavy duty knife, maybe even the TC used for undecalcified bone sections but if the samples are huge, it would require the macro system. I may be much easier to do paraffin sections and NOT cryosections. The samples would have to be thawed, simply drop into formalin, fix and process with a longer schedule to ensure good dehydration, clearing, but most of all infiltration of paraffin into the cartilage. At 10:52 AM 5/2/2006, you wrote: >Can anyone suggest a resource for getting frozen sections of Elk Larynx? > >One of my clients asks: >"I study elk sound production. As one question we investigate the anatomical >structure of the larynx. For this purpose I need cross sections of several >larynges (approx. 15 larynges). Cross sections at three positions are >necessary for each larynx,. The larynges are most likely calcified at >varying degrees. I have stored the larynges frozen in saline solution." > >Patsy Ruegg, HT(ASCP)QIHC >IHCtech, LLC >Fitzsimmons BioScience Park >12635 Montview Blvd. Suite 215 >Aurora, CO 80010 >P-720-859-4060 >F-720-859-4110 >wk email pruegg@ihctech.net >web site www.ihctech.net > > >This email is confidential and intended solely for the use of the Person(s) >('the intended recipient') to whom it was addressed. Any views or opinions >presented are solely those of the author. It may contain information that is >privileged & confidential within the meaning of applicable law. Accordingly >any dissemination, distribution, copying, or other use of this message, or >any of its contents, by any person other than the intended recipient may >constitute a breach of civil or criminal law and is strictly prohibited. If >you are NOT the intended recipient please contact the sender and dispose of >this e-mail as soon as possible. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From RRA <@t> Stowers-Institute.org Tue May 2 13:32:41 2006 From: RRA <@t> Stowers-Institute.org (Allen, Rhonda) Date: Tue May 2 13:33:11 2006 Subject: [Histonet] Question Message-ID: There is a brain mold available from EMS. Look under Matrices and they have different kinds of matrices. Rhonda Allen BA HT(ASCP)HTL, QIHC Histotechnology Specialist II Stowers Institute 1000 E. 50th Street Kansas City, MO 64110 816-926-4305 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Tuesday, May 02, 2006 1:04 PM To: Nita Searcy; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Question The only thing I can think of is what literally looks like an "egg slicer" I think we had ordered it from MOPEC or one of the other surgical/autopsy companies some 6 years ago. It's a metal egg shaped base, and using hi-profile microtome blades you could slice fresh soft tissue (like brain)in thin even pieces. I haven't seen one in sometime, but maybe someone who reads my e-mail will know where they can be purchased these days. If I remember correctly back then it cost about $100. Good luck Vikki --- Nita Searcy wrote: > Anyone know of an inexpensive tool for cutting > "fresh" 1 mm sections? > Researcher wants to know. > > Thanks > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Tue May 2 13:24:20 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/NCID/VR)) Date: Tue May 2 13:34:46 2006 Subject: [Histonet] Question Message-ID: Check with Sakura regarding their grossing tools. Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Tuesday, May 02, 2006 1:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question Anyone know of an inexpensive tool for cutting "fresh" 1 mm sections? Researcher wants to know. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mauger <@t> email.chop.edu Tue May 2 13:42:30 2006 From: mauger <@t> email.chop.edu (Joanne Mauger) Date: Tue May 2 13:43:09 2006 Subject: [Histonet] Autostainer tubes Message-ID: Patti, Labvision sells the identical vials, in fact I was told they make the autostainer for Dako- www.labvision.com Jo >>> Patti Loykasek 05/02/06 1:31 PM >>> Opps - didn't put in there a supplier other than Dako. We had a backorder problem with Dako on these. Actually turned out to be a glitch in their system, so all is well. Thanks for all of the good info, though. Histotechs are a nice bunch of people! Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histology <@t> gradymem.org Tue May 2 14:03:38 2006 From: histology <@t> gradymem.org (histology@gradymem.org) Date: Tue May 2 14:04:06 2006 Subject: [Histonet] POC for Genetic Studies Message-ID: <52c3d553056d.53056d52c3d5@onenet.net> The POC goes through Pathology here and we send it out. Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org ----- Original Message ----- From: Laurie Colbert Date: Thursday, April 27, 2006 1:34 pm Subject: [Histonet] POC for Genetic Studies > This question is for those of you who work in hospitals and send > out POC tissue for genetic studies/chromosome analysis. > > Does the tissue get sent out directly from Labor and Delivery, or > does the tissue go through Pathology and Pathology sends it out? > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From lhotaks <@t> mcmaster.ca Tue May 2 15:17:32 2006 From: lhotaks <@t> mcmaster.ca (Sarka Lhotak) Date: Tue May 2 15:17:36 2006 Subject: [Histonet] antibodies to eIF2 alpha and PPAR gamma Message-ID: Hello Netters, I am looking for antibodies to phosphorylated eIF2-alpha and PPAR-gamma that work on mouse tissue, FFPE preferentially. I have tried some with all kinds of conditions and retrievals with no success. Is anybody doing this? Thanks a lot, desperately, Sarka Lhotak McMaster University Hamilton, Ontario From lhotaks <@t> mcmaster.ca Tue May 2 15:28:32 2006 From: lhotaks <@t> mcmaster.ca (Sarka Lhotak) Date: Tue May 2 15:28:35 2006 Subject: [Histonet] IHC on frozens Message-ID: Hello Netters, I am trying to get some antibodies work on mouse tissue (see my previous posting). Since I have not been successful on paraffin sections I now turned to frozens where I have no experience. I am getting the same staining (or background of course) on positive and negative slides (antibody omitted). Even antibodies that I know work very well on paraffin (my positive control), give me no specific staining and a background mainly on connective tissue. I've searched for a proper fixing, drying, dipping in water etc. procedures prior to staining. I found it very confusing, people swear by different, even opposing protocols. So I have a few questions: What is the best way to prepare/store your sections prior to staining? Is quenching peroxidase and blocking the same as on paraffin? Generally, are the primary and secondary antibody concentrations the same as on paraffin? Is the MOM problem worse on frozens than on paraffin? All suggestions are welcome. Desperately, Sarka Lhotak McMaster University Hamilton, Ontario From Linresearch <@t> aol.com Tue May 2 17:14:17 2006 From: Linresearch <@t> aol.com (Linresearch@aol.com) Date: Tue May 2 17:14:31 2006 Subject: Fwd: [Histonet] CD Abs Message-ID: <241.a7d80e3.318933b9@aol.com> From liz <@t> premierlab.com Tue May 2 17:31:31 2006 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue May 2 17:31:55 2006 Subject: [Histonet] gallocyanin stain for nissel Message-ID: <000a01c66e38$29664530$0300a8c0@Chlipala> I need to perform a gallocyanin stain for nissel on mouse lumbar spinal cord. I have a protocol from bancroft and it says to stain for 18-48 hours. Can anyone out there give me any pointers on this stain. I also need to order the gallocyanin and and the only reagent I see is gallocyanine is that the same thing? thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 From jhabecke <@t> fhcrc.org Tue May 2 17:42:35 2006 From: jhabecke <@t> fhcrc.org (Randolph-Habecker, Julie) Date: Tue May 2 17:42:42 2006 Subject: [Histonet] RANK, ER and PR in mouse Message-ID: <21F1955FA284134E981948D6D3FFD0420433EF07@harpo.fhcrc.org> Histonetters, Can anyone suggest antibodies to detect Rank, ER, and PR in mouse? If you have a protocol that you can share, that would be great too! Thanks, Julie Julie Randolph-Habecker, Ph.D. Staff Scientist - Manager Experimental Histopathology Shared Resource Fred Hutchinson Cancer Research Center 1100 Fairview Ave, N. M5-A803 Seattle WA 98109-1024 206-667-6119 jhabecke@fhcrc.org From ynwang <@t> u.washington.edu Tue May 2 19:02:20 2006 From: ynwang <@t> u.washington.edu (Yak-Nam Wang) Date: Tue May 2 19:01:11 2006 Subject: [Histonet] NADH staining Message-ID: Everyone, I want to do some NADH staining to determine cell viability in treated porcine tissue (liver to start with). However, I do not know which reduced NADH I should be using to make up my solution: alpha or beta. I have seen papers using both types. Beta-NADH seems to be a lot cheaper but will it make a difference which one I use? Thank you for your help Yak-Nam -- Research Associate CIMU Applied Physics Laboratory University of Washington Box 355640 Seattle WA 98195 Tel.: 206-616-6673 From jkiernan <@t> uwo.ca Wed May 3 00:20:48 2006 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed May 3 00:19:41 2006 Subject: [Histonet] gallocyanine stain for Nissl Message-ID: <44583DB0.9060804@uwo.ca> The procedure in Bancroft & Gamble is OK. You can shorten the staining time to 2 hours by doing it in a 60C oven instead of at room temperature. (A higher temperature may shorten the shelf-life of the staining solution. Has anyone tested this?) The correct spelling is gallocyanine (because the dye is an amine). The CI number is 51030 (CI Mordant blue 10). The chemistry of the cationic chromium complex of the dye and its binding to nucleic acids is well understood - supported by various studies more recent than the two cited in B & G. This is a truly excellent staining method for anyone who's not in a hurry. Its big advantages over most other Nissl stains (such as cresyl violet, toluidine blue and neutral red) are that there is no overstaining and the colour resists most subsequently applied chemicals such as alcohol-water mixtures and acidic solutions of anionic dyes. Simple basic dyes, intelligently applied, can provide fast Nissl staining for small batches of slides. With chrome-gallocyanine there is no need for speed or intelligence; that's why I really like the method, even though the quality of the colour (hue? saturation?) is a rather bland greyish-blue. The only shortcoming of the gallocyanine-chrome alum method is that the staining solution begins to deteriorate after 1-2 weeks. Because maximum coloration is confined to the stainable substances (in the CNS this means nuclear DNA and ribosomal RNA) it's possible to do mass-production staining without having to worry about shelf-life. A 400 ml batch of gallocyanine-chrome alum solution can be used repeatedly to stain several racks of slides for a few days. Throw the staining solution out when you've finished the batch, because if you try to use it again two weeks later it will be no good. (Guess how I found that out.) The intensity of cytoplasmic staining with gallocyanine-chrome alum has been shown by scanning microspectrophotometry to be proportional to the concentration of what we now call rRNA. Einarson's 1951 paper, which is cited in Bancroft & Gamble, is a good read, and it shows that there was molecular biology 13 years before anyone had seen a ribosome. By the late 1950s it was known that cells contained more RNA when they were making more protein. Einarson's method, dating from 1932, did its bit and is still useful. John Kiernan Anatomy, UWO London, Canada. -------------------------- Elizabeth Chlipala wrote: > > I need to perform a gallocyanin stain for nissel on mouse lumbar spinal > cord. I have a protocol from bancroft and it says to stain for 18-48 > hours. Can anyone out there give me any pointers on this stain. I also > need to order the gallocyanin and and the only reagent I see is > gallocyanine is that the same thing? > > thanks in advance > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Manager > Premier Laboratory, LLC > P.O. Box 18592 > Boulder, Colorado 80308 > Office: (303) 735-5001 > Fax: (303) 735-3540 > liz@premierlab.com > www.premierlab.com > --- From mrcraigwbarlow <@t> googlemail.com Wed May 3 05:50:42 2006 From: mrcraigwbarlow <@t> googlemail.com (CRAIG BARLOW) Date: Wed May 3 05:50:48 2006 Subject: [Histonet] Inflamatory markers Message-ID: <22482b560605030350v72081d43la6d8f14476551fc5@mail.gmail.com> Hello All, I am looking for inflammatory markers in skin that I can perform quantitative IHC on. I have a few in mind i.e CD68 just thought I'd throw the question out? All replies are welcome. Regards Craig Barlow From Jacqueline.Malam <@t> rli.mbht.nhs.uk Wed May 3 06:14:12 2006 From: Jacqueline.Malam <@t> rli.mbht.nhs.uk (Malam Jacqueline) Date: Wed May 3 06:15:19 2006 Subject: [Histonet] Re- processing fatty tissues Message-ID: We have used a modified routine method for a few years now and it works fine with our Shandon Pathcentre processor as long as you keep an eye on the number of blocks processed and change reagents accordingly, and the blocks don't exceed recommended size and thickness. Schedule - Neutral buffered 10% formalin - 11/2 hrs at room temp (unless you have a downdraft extractor then use heat) 70% alc, 90% alc then 3 changes of 100% alc (alcohols) - 1 hr all heated to 40 C 1 change of 100% alcohol/xylene mixed ratio of 50:50 - 1 hr heated to 40 C 3 changes of xylene - 1 hr 15 mins all heated to 40 C 4 changes of wax - 1 hr 15 mins all heated to 60 C Jacqui Malam Lancaster UK DISCLAIMER: This e-mail is confidential and privileged. If you are not the intended recipient please accept our apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform postmaster@rli.mbht.nhs.uk that this message has gone astray before deleting it. Comments or opinions expressed in this email are those of their respective contributors only. The views expressed do not represent the views of the Trust, its management or employees. University Hospitals of Morecambe Bay NHS Trust is not responsible and disclaims any and all liability for the content of comments written within.Thank you for your co-operation. From KevinMcGovern <@t> catholichealth.net Wed May 3 08:12:12 2006 From: KevinMcGovern <@t> catholichealth.net (McGovern, Kevin) Date: Wed May 3 08:12:29 2006 Subject: [Histonet] The end of an era Message-ID: Kindly remove me from your mailing list. Thank you. From mward <@t> wfubmc.edu Wed May 3 09:47:40 2006 From: mward <@t> wfubmc.edu (Martha Ward) Date: Wed May 3 09:47:48 2006 Subject: [Histonet] Mycobacterium bovis (BCG) antibody Message-ID: <61135F0455D33347B5AAE209B903A30413CCCE11@EXCHVS2.medctr.ad.wfubmc.edu> My dermatopathologist asked me to look into this antibody to add to our menu to ccreen for microorganisms. I would appreciate any advice concerning vendors, conditions, etc. Thanks in advance for your help. Martha Ward Molecular Diagnostics Lab Wake Forest University Baptist Medical Center From kab35 <@t> cornell.edu Wed May 3 10:10:18 2006 From: kab35 <@t> cornell.edu (Kathie A Berghorn) Date: Wed May 3 10:10:24 2006 Subject: [Histonet] Glycerol cryoprotected tissue and cryostat sectioning Message-ID: Dear Histo-netters, I need your help. We have precious tissue that has been processed for beta-galactosidase. This was the first time we did this immunohistochemistry. The protocol said to clear in glycerol which we did and the tissue is in 80% glycerol. I have spent the morning trying to cut this tissue on a cryostat without success. When I put the mouse placenta in OCT using isopentane and liquid nitrogen, the OCT freezes but the tissue does not. Cutting it on the cryostat is then not possible. When I used to do thick sections we sunk tissue in sucrose and then froze it with dry ice prior to cutting it on a freezing sliding microtome. Would the dry ice freeze the placenta in the cryostat so I could cut it? Any other suggestions? Of course I am on a grant deadline and am stressing. I would appreciate any suggestions that would yield 8-10 ?m sections so I can see if the beta galactosidase worked in the area of the placenta we study. Thank you very much, Kathie From gpbnas <@t> yahoo.es Wed May 3 10:46:18 2006 From: gpbnas <@t> yahoo.es (Guillermo Palao) Date: Wed May 3 10:46:23 2006 Subject: [Histonet] IHC on frozens In-Reply-To: Message-ID: <20060503154618.25177.qmail@web26211.mail.ukl.yahoo.com> Hi Sarka, I have recently had similar problems with parafin vs frozen sections, and these are the conclusions I have come to: - We routinely store at -20 ?C fridge the frozen sections already fixed for 5 min in acetone at -20 ?C, I do not know if this is the best way to do it, and I would appreciate any more informed input in this sense. - For quenching endogenous peroxidase you may use H2O2 0.3% (instead of 3%) for 5 min RT (instead of 30 min). - In my (little) experience, Ab concentrations do not matter that much if the antibody is good. For convenience purposes I stain frozen sections for 1 hour RT as compared with O/N at 4 ?C for FFPE sections. - We noticed similar (if not worse) background problems due to endogenous mouse IgG in frozen sections, although I did not do a side by side comparison. I found a really good improvement in terms of background after blocking endogenous biotin with Vector Avidin/Biotin blocking kit. Try staining your slides only with ABC reagent (no Abs at all), and if you see color developing (as was my case) then you definitely need to block endogenous biotin. Good luck, Guillermo Sarka Lhotak escribi?: Hello Netters, I am trying to get some antibodies work on mouse tissue (see my previous posting). Since I have not been successful on paraffin sections I now turned to frozens where I have no experience. I am getting the same staining (or background of course) on positive and negative slides (antibody omitted). Even antibodies that I know work very well on paraffin (my positive control), give me no specific staining and a background mainly on connective tissue. I've searched for a proper fixing, drying, dipping in water etc. procedures prior to staining. I found it very confusing, people swear by different, even opposing protocols. So I have a few questions: What is the best way to prepare/store your sections prior to staining? Is quenching peroxidase and blocking the same as on paraffin? Generally, are the primary and secondary antibody concentrations the same as on paraffin? Is the MOM problem worse on frozens than on paraffin? All suggestions are welcome. Desperately, Sarka Lhotak McMaster University Hamilton, Ontario _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- LLama Gratis a cualquier PC del Mundo. Llamadas a fijos y m?viles desde 1 c?ntimo por minuto. http://es.voice.yahoo.com From pmarcum <@t> vet.upenn.edu Wed May 3 10:49:24 2006 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Wed May 3 10:49:33 2006 Subject: [Histonet] Save These Dates For The Pennsylvania State Histology Meeting Message-ID: <6.1.1.1.2.20060503113716.01a00340@mail.vet.upenn.edu> Good Morning to All, We are having the PSH fall meeting in Pittsburgh this year with a special seminars series on Thursday, October 26th in the afternoon for Histology Supervisors and Managers to start the meeting. We will offer a CPT Coding Seminar, a CAP Inspection Seminar and Smart Shopping for Histology For Contracts Negotiation and Purchasing. Friday, October 27th we will have a full schedule of speakers for general and special histology/cytology topics be announced later. Saturday October 28th we will offer HT Readiness for ASCP Testing (All Day) and QIHC Readiness Testing (All Day) along with special speakers from the forensics CSI area throughout the day. We will have topics for routine and research histology all day. Join us October 26 to 28, 2006 in Pittsburgh PA. We are contacting everyone we can in nine states to join us. We are still early enough in the process to accept suggestions for topics and speakers for 3 time slots. If you have a suggestion please let me know and we will consider it. Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From liz <@t> premierlab.com Wed May 3 10:49:43 2006 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed May 3 10:50:07 2006 Subject: [Histonet] Mycobacterium bovis (BCG) antibody In-Reply-To: <61135F0455D33347B5AAE209B903A30413CCCE11@EXCHVS2.medctr.ad.wfubmc.edu> Message-ID: <001901c66ec9$32794cd0$0300a8c0@Chlipala> Martha We haved used this antibody in research quite a bit on human, mouse and guinea pig specimens of tuberculosis. We used the antibody from Dako. It works quite well on FFPE tissue, but there is one thing that you need to be aware of and that it is not entirely specific to MTB it will also stain other gram positive bacillia. I believe that is stated on the spec sheet. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward Sent: Wednesday, May 03, 2006 8:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mycobacterium bovis (BCG) antibody My dermatopathologist asked me to look into this antibody to add to our menu to ccreen for microorganisms. I would appreciate any advice concerning vendors, conditions, etc. Thanks in advance for your help. Martha Ward Molecular Diagnostics Lab Wake Forest University Baptist Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1.1517 (20060502) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From dlcowie <@t> prodigy.net Wed May 3 12:06:21 2006 From: dlcowie <@t> prodigy.net (Dawn Cowie) Date: Wed May 3 12:06:26 2006 Subject: [Histonet] supv. position open Message-ID: <20060503170621.60546.qmail@web81007.mail.mud.yahoo.com> hello histonetters, Histology supervisor position available. Mid size private lab located in the Florida panhandle. Must be ASCP certified at the HT or HTL level and qualify for at least Florida HTL license, preferably Florida Supervisor license. Approx. 40,000 surgical cases per year, 100,000 blocks, 11,000 IHC, 8,500 special stains per year. For more details, please call me at 850-416-7251 or e-mail me at dlcowie@prodigy.net Dawn Cowie HT (ASCP), HTL, S Histology Supervisor Pensacola Pathologists, PA From NBerghoff <@t> cvm.tamu.edu Wed May 3 12:23:01 2006 From: NBerghoff <@t> cvm.tamu.edu (Nora Berghoff) Date: Wed May 3 12:23:35 2006 Subject: [Histonet] Accidentally frozen NBF fixed tissue Message-ID: Hello all, Someone in our lab accidentally froze 10% NBF fixed pancreas at -80 degree C. They now want to get slides for H&E etc off that. Does anyone know whether this tissue is still usable after being frozen? Obviously it is not good to freeze fixed tissue, but has anyone ever done that and still gotten usable results? I would appreciate any input! Thanks so much, Nora Berghoff ************************************** Nora Berghoff, med. vet. Research Assistant Gastrointestinal Laboratory College of Veterinary Medicine Texas A&M University Phone: (979) 458 2293 (w) ************************************* From 1dpeterson <@t> meriter.com Wed May 3 12:42:52 2006 From: 1dpeterson <@t> meriter.com (Peterson, Dan) Date: Wed May 3 12:42:56 2006 Subject: [Histonet] Storage question Message-ID: <328CBAE62F31C642B422970E879DFADC01A80089@pcwex01> We've recently been cited by our on-site safety committee for not having ALL of our specimen containers labeled as containing formalin. Anything we send out from the lab to outside accounts, all have a warning label. However, the specimens we collect in-house are placed into plastic containers and filled appropriately with formalin. We're talking 40-50 a day. Our staff are the only people to handle the containers from fill to disposal. I'm just curious as to how other labs are handling this. I would like to place the HCS label on the outside of the cabinets where the tissue is stored. We have formaldehyde warning placards on every door entering the lab. It just seems like a waste of time and money (for labels) for an unwarranted worry that someone can get into somewhere they don't belong and mess with something they have no idea of what they are messing with. Daniel R Peterson HT(ASCP) Histopathology Section Head General Medical Laboratories (608)-267-6557 1dpeterson@meriter.com CONFIDENTIALITY NOTICE: This message (including any attachments) is intended for the sole use of the individual and entity to whom it is addressed. This message may contain information that is confidential and is protected by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply email and then delete the message. Thank you. From stephanie.brusig <@t> weyerhaeuser.com Wed May 3 12:43:22 2006 From: stephanie.brusig <@t> weyerhaeuser.com (Brusig, Stephanie) Date: Wed May 3 12:43:32 2006 Subject: [Histonet] RE: Anyone use glass knifes anymore? Message-ID: <16E971922EDF9A4B9E8D3216374EDD49012B7B62@wafedixm12.corp.weyer.pri> I make and use glass knifes for plastic sectioning at 3 microns. I usually have to go through a whole glass strip to get one good knife. Does anyone make them anymore and have the same problem? ~Stephanie Brusig Weyerhaeuser Company Propagation of High Value Trees 32901 Weyerhaeuser Way S WTC-1B10 Federal Way, WA 98001 253.924.6518 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, May 03, 2006 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 30, Issue 3 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. CONTROL BLOCKS NEEDED (Nymeyer, Heather) 2. Large sections (Patsy Ruegg) 3. Autostainer tubes (Patti Loykasek) 4. NSH Active Members (Carrie Diamond) 5. Question (Nita Searcy) 6. Re: Question (Victoria Baker) 7. Re: Large sections (Gayle Callis) 8. RE: Question (Allen, Rhonda) 9. RE: Question (Bartlett, Jeanine (CDC/NCID/VR)) 10. Re: Autostainer tubes (Joanne Mauger) 11. Re: POC for Genetic Studies (histology@gradymem.org) 12. antibodies to eIF2 alpha and PPAR gamma (Sarka Lhotak) 13. IHC on frozens (Sarka Lhotak) 14. Fwd: [Histonet] CD Abs (Linresearch@aol.com) 15. gallocyanin stain for nissel (Elizabeth Chlipala) 16. RANK, ER and PR in mouse (Randolph-Habecker, Julie) 17. NADH staining (Yak-Nam Wang) 18. Re: gallocyanine stain for Nissl (John Kiernan) 19. Inflamatory markers (CRAIG BARLOW) 20. Re- processing fatty tissues (Malam Jacqueline) 21. The end of an era (McGovern, Kevin) 22. Mycobacterium bovis (BCG) antibody (Martha Ward) 23. Glycerol cryoprotected tissue and cryostat sectioning (Kathie A Berghorn) 24. RE: IHC on frozens (Guillermo Palao) 25. Save These Dates For The Pennsylvania State Histology Meeting (Pamela Marcum) 26. RE: Mycobacterium bovis (BCG) antibody (Elizabeth Chlipala) ---------------------------------------------------------------------- Message: 1 Date: Tue, 2 May 2006 10:03:23 -0700 From: "Nymeyer, Heather" Subject: [Histonet] CONTROL BLOCKS NEEDED To: Message-ID: <463911DE3F9F8D4AA3EC67632386BC62D1509E@dc1serv78.interiorhealth.ca> Content-Type: text/plain; charset="us-ascii" We are in need of the following control blocks - Leprosy bacilli - Spirochete Your assistance is appreciated and thank you in advance. Heather D. Nymeyer, RT, CEBT Charge Technologist, Anatomic Pathology Royal Inland Hospital, Kamloops, BC. 250-314-2664 ------------------------------ Message: 2 Date: Tue, 2 May 2006 10:52:50 -0600 From: "Patsy Ruegg" Subject: [Histonet] Large sections To: Message-ID: <200605021652.k42Gqoog014137@chip.viawest.net> Content-Type: text/plain; charset="US-ASCII" Can anyone suggest a resource for getting frozen sections of Elk Larynx? One of my clients asks: "I study elk sound production. As one question we investigate the anatomical structure of the larynx. For this purpose I need cross sections of several larynges (approx. 15 larynges). Cross sections at three positions are necessary for each larynx,. The larynges are most likely calcified at varying degrees. I have stored the larynges frozen in saline solution." Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. ------------------------------ Message: 3 Date: Tue, 02 May 2006 10:31:31 -0700 From: Patti Loykasek Subject: [Histonet] Autostainer tubes To: histonet Message-ID: Content-Type: text/plain; charset="US-ASCII" Opps - didn't put in there a supplier other than Dako. We had a backorder problem with Dako on these. Actually turned out to be a glitch in their system, so all is well. Thanks for all of the good info, though. Histotechs are a nice bunch of people! Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. ------------------------------ Message: 4 Date: Tue, 2 May 2006 13:37:13 -0400 From: "Carrie Diamond" Subject: [Histonet] NSH Active Members To: Message-ID: Content-Type: text/plain; charset="us-ascii" NSH Active Members: Earlier this week you received a ballot and candidate information for the NSH 2006 Election. Unfortunately, an error occurred at the mail house. The mailing lists were incorrectly matched to the wrong region director information. It lists the incorrect region for which you are eligible to vote. We apologize for this mistake and have mailed a corrected ballot indicating the region director who is running for the position within your region. The ballot has been printed on cream colored paper and will be the only ballot counted from your region. We do hope you will return your ballot at your earliest convenience to the Nominations-Election Committee. Carrie Diamond Executive Director National Society for Histotechnology 4201 Northview Drive, Suite 502 Bowie, MD 20716 P: 301-262-6221 E: carrie@nsh.org Join us for the 32nd Annual Symposium Convention Phoenix, AZ September 8-13, 2006 Registration Details will be available in March ------------------------------ Message: 5 Date: Tue, 02 May 2006 12:49:01 -0500 From: "Nita Searcy" Subject: [Histonet] Question To: Message-ID: Content-Type: text/plain; charset=US-ASCII Anyone know of an inexpensive tool for cutting "fresh" 1 mm sections? Researcher wants to know. Thanks ------------------------------ Message: 6 Date: Tue, 2 May 2006 11:03:59 -0700 (PDT) From: Victoria Baker Subject: Re: [Histonet] Question To: Nita Searcy , histonet@lists.utsouthwestern.edu Message-ID: <20060502180359.42479.qmail@web52513.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 The only thing I can think of is what literally looks like an "egg slicer" I think we had ordered it from MOPEC or one of the other surgical/autopsy companies some 6 years ago. It's a metal egg shaped base, and using hi-profile microtome blades you could slice fresh soft tissue (like brain)in thin even pieces. I haven't seen one in sometime, but maybe someone who reads my e-mail will know where they can be purchased these days. If I remember correctly back then it cost about $100. Good luck Vikki --- Nita Searcy wrote: > Anyone know of an inexpensive tool for cutting "fresh" 1 mm sections? > Researcher wants to know. > > Thanks > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com ------------------------------ Message: 7 Date: Tue, 02 May 2006 12:31:46 -0600 From: Gayle Callis Subject: Re: [Histonet] Large sections To: "Patsy Ruegg" , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20060502122207.01b50218@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed I don't think the larynx will be calcified, but it will contain both hyaline and elastic cartilage which could be pretty tough to cryosection. The Instrumedics should be able to do the job with a heavy duty knife, maybe even the TC used for undecalcified bone sections but if the samples are huge, it would require the macro system. I may be much easier to do paraffin sections and NOT cryosections. The samples would have to be thawed, simply drop into formalin, fix and process with a longer schedule to ensure good dehydration, clearing, but most of all infiltration of paraffin into the cartilage. At 10:52 AM 5/2/2006, you wrote: >Can anyone suggest a resource for getting frozen sections of Elk Larynx? > >One of my clients asks: >"I study elk sound production. As one question we investigate the anatomical >structure of the larynx. For this purpose I need cross sections of several >larynges (approx. 15 larynges). Cross sections at three positions are >necessary for each larynx,. The larynges are most likely calcified at >varying degrees. I have stored the larynges frozen in saline solution." > >Patsy Ruegg, HT(ASCP)QIHC >IHCtech, LLC >Fitzsimmons BioScience Park >12635 Montview Blvd. Suite 215 >Aurora, CO 80010 >P-720-859-4060 >F-720-859-4110 >wk email pruegg@ihctech.net >web site www.ihctech.net > > >This email is confidential and intended solely for the use of the Person(s) >('the intended recipient') to whom it was addressed. Any views or opinions >presented are solely those of the author. It may contain information that is >privileged & confidential within the meaning of applicable law. Accordingly >any dissemination, distribution, copying, or other use of this message, or >any of its contents, by any person other than the intended recipient may >constitute a breach of civil or criminal law and is strictly prohibited. If >you are NOT the intended recipient please contact the sender and dispose of >this e-mail as soon as possible. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) ------------------------------ Message: 8 Date: Tue, 2 May 2006 13:32:41 -0500 From: "Allen, Rhonda" Subject: RE: [Histonet] Question To: "Victoria Baker" , "Nita Searcy" , Message-ID: Content-Type: text/plain; charset="us-ascii" There is a brain mold available from EMS. Look under Matrices and they have different kinds of matrices. Rhonda Allen BA HT(ASCP)HTL, QIHC Histotechnology Specialist II Stowers Institute 1000 E. 50th Street Kansas City, MO 64110 816-926-4305 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Tuesday, May 02, 2006 1:04 PM To: Nita Searcy; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Question The only thing I can think of is what literally looks like an "egg slicer" I think we had ordered it from MOPEC or one of the other surgical/autopsy companies some 6 years ago. It's a metal egg shaped base, and using hi-profile microtome blades you could slice fresh soft tissue (like brain)in thin even pieces. I haven't seen one in sometime, but maybe someone who reads my e-mail will know where they can be purchased these days. If I remember correctly back then it cost about $100. Good luck Vikki --- Nita Searcy wrote: > Anyone know of an inexpensive tool for cutting > "fresh" 1 mm sections? > Researcher wants to know. > > Thanks > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Tue, 2 May 2006 14:24:20 -0400 From: "Bartlett, Jeanine \(CDC/NCID/VR\)" Subject: RE: [Histonet] Question To: "Nita Searcy" , Message-ID: Content-Type: text/plain; charset="us-ascii" Check with Sakura regarding their grossing tools. Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Tuesday, May 02, 2006 1:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question Anyone know of an inexpensive tool for cutting "fresh" 1 mm sections? Researcher wants to know. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 02 May 2006 14:42:30 -0400 From: "Joanne Mauger" Subject: Re: [Histonet] Autostainer tubes To: , Message-ID: Content-Type: text/plain; charset=US-ASCII Patti, Labvision sells the identical vials, in fact I was told they make the autostainer for Dako- www.labvision.com Jo >>> Patti Loykasek 05/02/06 1:31 PM >>> Opps - didn't put in there a supplier other than Dako. We had a backorder problem with Dako on these. Actually turned out to be a glitch in their system, so all is well. Thanks for all of the good info, though. Histotechs are a nice bunch of people! Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Tue, 02 May 2006 14:03:38 -0500 From: histology@gradymem.org Subject: Re: [Histonet] POC for Genetic Studies To: Laurie Colbert Cc: histonet@lists.utsouthwestern.edu Message-ID: <52c3d553056d.53056d52c3d5@onenet.net> Content-Type: text/plain; charset=us-ascii The POC goes through Pathology here and we send it out. Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org ----- Original Message ----- From: Laurie Colbert Date: Thursday, April 27, 2006 1:34 pm Subject: [Histonet] POC for Genetic Studies > This question is for those of you who work in hospitals and send > out POC tissue for genetic studies/chromosome analysis. > > Does the tissue get sent out directly from Labor and Delivery, or > does the tissue go through Pathology and Pathology sends it out? > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 12 Date: Tue, 02 May 2006 16:17:32 -0400 From: "Sarka Lhotak" Subject: [Histonet] antibodies to eIF2 alpha and PPAR gamma To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="ISO-8859-1" Hello Netters, I am looking for antibodies to phosphorylated eIF2-alpha and PPAR-gamma that work on mouse tissue, FFPE preferentially. I have tried some with all kinds of conditions and retrievals with no success. Is anybody doing this? Thanks a lot, desperately, Sarka Lhotak McMaster University Hamilton, Ontario ------------------------------ Message: 13 Date: Tue, 02 May 2006 16:28:32 -0400 From: "Sarka Lhotak" Subject: [Histonet] IHC on frozens To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="ISO-8859-1" Hello Netters, I am trying to get some antibodies work on mouse tissue (see my previous posting). Since I have not been successful on paraffin sections I now turned to frozens where I have no experience. I am getting the same staining (or background of course) on positive and negative slides (antibody omitted). Even antibodies that I know work very well on paraffin (my positive control), give me no specific staining and a background mainly on connective tissue. I've searched for a proper fixing, drying, dipping in water etc. procedures prior to staining. I found it very confusing, people swear by different, even opposing protocols. So I have a few questions: What is the best way to prepare/store your sections prior to staining? Is quenching peroxidase and blocking the same as on paraffin? Generally, are the primary and secondary antibody concentrations the same as on paraffin? Is the MOM problem worse on frozens than on paraffin? All suggestions are welcome. Desperately, Sarka Lhotak McMaster University Hamilton, Ontario ------------------------------ Message: 14 Date: Tue, 2 May 2006 18:14:17 EDT From: Linresearch@aol.com Subject: Fwd: [Histonet] CD Abs To: histonet@pathology.swmed.edu Message-ID: <241.a7d80e3.318933b9@aol.com> Content-Type: text/plain; charset="us-ascii" ------------------------------ Message: 15 Date: Tue, 2 May 2006 16:31:31 -0600 From: "Elizabeth Chlipala" Subject: [Histonet] gallocyanin stain for nissel To: Message-ID: <000a01c66e38$29664530$0300a8c0@Chlipala> Content-Type: text/plain; charset="US-ASCII" I need to perform a gallocyanin stain for nissel on mouse lumbar spinal cord. I have a protocol from bancroft and it says to stain for 18-48 hours. Can anyone out there give me any pointers on this stain. I also need to order the gallocyanin and and the only reagent I see is gallocyanine is that the same thing? thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 ------------------------------ Message: 16 Date: Tue, 2 May 2006 15:42:35 -0700 From: "Randolph-Habecker, Julie" Subject: [Histonet] RANK, ER and PR in mouse To: Message-ID: <21F1955FA284134E981948D6D3FFD0420433EF07@harpo.fhcrc.org> Content-Type: text/plain; charset="us-ascii" Histonetters, Can anyone suggest antibodies to detect Rank, ER, and PR in mouse? If you have a protocol that you can share, that would be great too! Thanks, Julie Julie Randolph-Habecker, Ph.D. Staff Scientist - Manager Experimental Histopathology Shared Resource Fred Hutchinson Cancer Research Center 1100 Fairview Ave, N. M5-A803 Seattle WA 98109-1024 206-667-6119 jhabecke@fhcrc.org ------------------------------ Message: 17 Date: Tue, 02 May 2006 17:02:20 -0700 From: Yak-Nam Wang Subject: [Histonet] NADH staining To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Everyone, I want to do some NADH staining to determine cell viability in treated porcine tissue (liver to start with). However, I do not know which reduced NADH I should be using to make up my solution: alpha or beta. I have seen papers using both types. Beta-NADH seems to be a lot cheaper but will it make a difference which one I use? Thank you for your help Yak-Nam -- Research Associate CIMU Applied Physics Laboratory University of Washington Box 355640 Seattle WA 98195 Tel.: 206-616-6673 ------------------------------ Message: 18 Date: Wed, 03 May 2006 01:20:48 -0400 From: John Kiernan Subject: Re: [Histonet] gallocyanine stain for Nissl To: Elizabeth Chlipala , Histonet listserver Message-ID: <44583DB0.9060804@uwo.ca> Content-Type: text/plain; charset=ISO-8859-1; format=flowed The procedure in Bancroft & Gamble is OK. You can shorten the staining time to 2 hours by doing it in a 60C oven instead of at room temperature. (A higher temperature may shorten the shelf-life of the staining solution. Has anyone tested this?) The correct spelling is gallocyanine (because the dye is an amine). The CI number is 51030 (CI Mordant blue 10). The chemistry of the cationic chromium complex of the dye and its binding to nucleic acids is well understood - supported by various studies more recent than the two cited in B & G. This is a truly excellent staining method for anyone who's not in a hurry. Its big advantages over most other Nissl stains (such as cresyl violet, toluidine blue and neutral red) are that there is no overstaining and the colour resists most subsequently applied chemicals such as alcohol-water mixtures and acidic solutions of anionic dyes. Simple basic dyes, intelligently applied, can provide fast Nissl staining for small batches of slides. With chrome-gallocyanine there is no need for speed or intelligence; that's why I really like the method, even though the quality of the colour (hue? saturation?) is a rather bland greyish-blue. The only shortcoming of the gallocyanine-chrome alum method is that the staining solution begins to deteriorate after 1-2 weeks. Because maximum coloration is confined to the stainable substances (in the CNS this means nuclear DNA and ribosomal RNA) it's possible to do mass-production staining without having to worry about shelf-life. A 400 ml batch of gallocyanine-chrome alum solution can be used repeatedly to stain several racks of slides for a few days. Throw the staining solution out when you've finished the batch, because if you try to use it again two weeks later it will be no good. (Guess how I found that out.) The intensity of cytoplasmic staining with gallocyanine-chrome alum has been shown by scanning microspectrophotometry to be proportional to the concentration of what we now call rRNA. Einarson's 1951 paper, which is cited in Bancroft & Gamble, is a good read, and it shows that there was molecular biology 13 years before anyone had seen a ribosome. By the late 1950s it was known that cells contained more RNA when they were making more protein. Einarson's method, dating from 1932, did its bit and is still useful. John Kiernan Anatomy, UWO London, Canada. -------------------------- Elizabeth Chlipala wrote: > > I need to perform a gallocyanin stain for nissel on mouse lumbar spinal > cord. I have a protocol from bancroft and it says to stain for 18-48 > hours. Can anyone out there give me any pointers on this stain. I also > need to order the gallocyanin and and the only reagent I see is > gallocyanine is that the same thing? > > thanks in advance > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Manager > Premier Laboratory, LLC > P.O. Box 18592 > Boulder, Colorado 80308 > Office: (303) 735-5001 > Fax: (303) 735-3540 > liz@premierlab.com > www.premierlab.com > --- ------------------------------ Message: 19 Date: Wed, 3 May 2006 11:50:42 +0100 From: "CRAIG BARLOW" Subject: [Histonet] Inflamatory markers To: histonet Message-ID: <22482b560605030350v72081d43la6d8f14476551fc5@mail.gmail.com> Content-Type: text/plain; charset=WINDOWS-1252; format=flowed Hello All, I am looking for inflammatory markers in skin that I can perform quantitative IHC on. I have a few in mind i.e CD68 just thought I'd throw the question out... All replies are welcome. Regards Craig Barlow ------------------------------ Message: 20 Date: Wed, 3 May 2006 12:14:12 +0100 From: Malam Jacqueline Subject: [Histonet] Re- processing fatty tissues To: "Histonet submissions (histonet@lists.utsouthwestern.edu)" Message-ID: Content-Type: text/plain We have used a modified routine method for a few years now and it works fine with our Shandon Pathcentre processor as long as you keep an eye on the number of blocks processed and change reagents accordingly, and the blocks don't exceed recommended size and thickness. Schedule - Neutral buffered 10% formalin - 11/2 hrs at room temp (unless you have a downdraft extractor then use heat) 70% alc, 90% alc then 3 changes of 100% alc (alcohols) - 1 hr all heated to 40 C 1 change of 100% alcohol/xylene mixed ratio of 50:50 - 1 hr heated to 40 C 3 changes of xylene - 1 hr 15 mins all heated to 40 C 4 changes of wax - 1 hr 15 mins all heated to 60 C Jacqui Malam Lancaster UK DISCLAIMER: This e-mail is confidential and privileged. If you are not the intended recipient please accept our apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform postmaster@rli.mbht.nhs.uk that this message has gone astray before deleting it. Comments or opinions expressed in this email are those of their respective contributors only. The views expressed do not represent the views of the Trust, its management or employees. University Hospitals of Morecambe Bay NHS Trust is not responsible and disclaims any and all liability for the content of comments written within.Thank you for your co-operation. ------------------------------ Message: 21 Date: Wed, 3 May 2006 08:12:12 -0500 From: "McGovern, Kevin" Subject: [Histonet] The end of an era To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=iso-8859-1 Kindly remove me from your mailing list. Thank you. ------------------------------ Message: 22 Date: Wed, 3 May 2006 10:47:40 -0400 From: "Martha Ward" Subject: [Histonet] Mycobacterium bovis (BCG) antibody To: Message-ID: <61135F0455D33347B5AAE209B903A30413CCCE11@EXCHVS2.medctr.ad.wfubmc.edu> Content-Type: text/plain; charset="us-ascii" My dermatopathologist asked me to look into this antibody to add to our menu to ccreen for microorganisms. I would appreciate any advice concerning vendors, conditions, etc. Thanks in advance for your help. Martha Ward Molecular Diagnostics Lab Wake Forest University Baptist Medical Center ------------------------------ Message: 23 Date: Wed, 3 May 2006 11:10:18 -0400 From: Kathie A Berghorn Subject: [Histonet] Glycerol cryoprotected tissue and cryostat sectioning To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" ; format="flowed" Dear Histo-netters, I need your help. We have precious tissue that has been processed for beta-galactosidase. This was the first time we did this immunohistochemistry. The protocol said to clear in glycerol which we did and the tissue is in 80% glycerol. I have spent the morning trying to cut this tissue on a cryostat without success. When I put the mouse placenta in OCT using isopentane and liquid nitrogen, the OCT freezes but the tissue does not. Cutting it on the cryostat is then not possible. When I used to do thick sections we sunk tissue in sucrose and then froze it with dry ice prior to cutting it on a freezing sliding microtome. Would the dry ice freeze the placenta in the cryostat so I could cut it? Any other suggestions? Of course I am on a grant deadline and am stressing. I would appreciate any suggestions that would yield 8-10 ?m sections so I can see if the beta galactosidase worked in the area of the placenta we study. Thank you very much, Kathie ------------------------------ Message: 24 Date: Wed, 3 May 2006 17:46:18 +0200 (CEST) From: Guillermo Palao Subject: RE: [Histonet] IHC on frozens To: Histonet Message-ID: <20060503154618.25177.qmail@web26211.mail.ukl.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hi Sarka, I have recently had similar problems with parafin vs frozen sections, and these are the conclusions I have come to: - We routinely store at -20 ?C fridge the frozen sections already fixed for 5 min in acetone at -20 ?C, I do not know if this is the best way to do it, and I would appreciate any more informed input in this sense. - For quenching endogenous peroxidase you may use H2O2 0.3% (instead of 3%) for 5 min RT (instead of 30 min). - In my (little) experience, Ab concentrations do not matter that much if the antibody is good. For convenience purposes I stain frozen sections for 1 hour RT as compared with O/N at 4 ?C for FFPE sections. - We noticed similar (if not worse) background problems due to endogenous mouse IgG in frozen sections, although I did not do a side by side comparison. I found a really good improvement in terms of background after blocking endogenous biotin with Vector Avidin/Biotin blocking kit. Try staining your slides only with ABC reagent (no Abs at all), and if you see color developing (as was my case) then you definitely need to block endogenous biotin. Good luck, Guillermo Sarka Lhotak escribi?: Hello Netters, I am trying to get some antibodies work on mouse tissue (see my previous posting). Since I have not been successful on paraffin sections I now turned to frozens where I have no experience. I am getting the same staining (or background of course) on positive and negative slides (antibody omitted). Even antibodies that I know work very well on paraffin (my positive control), give me no specific staining and a background mainly on connective tissue. I've searched for a proper fixing, drying, dipping in water etc. procedures prior to staining. I found it very confusing, people swear by different, even opposing protocols. So I have a few questions: What is the best way to prepare/store your sections prior to staining? Is quenching peroxidase and blocking the same as on paraffin? Generally, are the primary and secondary antibody concentrations the same as on paraffin? Is the MOM problem worse on frozens than on paraffin? All suggestions are welcome. Desperately, Sarka Lhotak McMaster University Hamilton, Ontario _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- LLama Gratis a cualquier PC del Mundo. Llamadas a fijos y m?viles desde 1 c?ntimo por minuto. http://es.voice.yahoo.com ------------------------------ Message: 25 Date: Wed, 03 May 2006 11:49:24 -0400 From: Pamela Marcum Subject: [Histonet] Save These Dates For The Pennsylvania State Histology Meeting To: histonet@lists.utsouthwestern.edu Message-ID: <6.1.1.1.2.20060503113716.01a00340@mail.vet.upenn.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Good Morning to All, We are having the PSH fall meeting in Pittsburgh this year with a special seminars series on Thursday, October 26th in the afternoon for Histology Supervisors and Managers to start the meeting. We will offer a CPT Coding Seminar, a CAP Inspection Seminar and Smart Shopping for Histology For Contracts Negotiation and Purchasing. Friday, October 27th we will have a full schedule of speakers for general and special histology/cytology topics be announced later. Saturday October 28th we will offer HT Readiness for ASCP Testing (All Day) and QIHC Readiness Testing (All Day) along with special speakers from the forensics CSI area throughout the day. We will have topics for routine and research histology all day. Join us October 26 to 28, 2006 in Pittsburgh PA. We are contacting everyone we can in nine states to join us. We are still early enough in the process to accept suggestions for topics and speakers for 3 time slots. If you have a suggestion please let me know and we will consider it. Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu ------------------------------ Message: 26 Date: Wed, 3 May 2006 09:49:43 -0600 From: "Elizabeth Chlipala" Subject: RE: [Histonet] Mycobacterium bovis (BCG) antibody To: "'Martha Ward'" , Message-ID: <001901c66ec9$32794cd0$0300a8c0@Chlipala> Content-Type: text/plain; charset="US-ASCII" Martha We haved used this antibody in research quite a bit on human, mouse and guinea pig specimens of tuberculosis. We used the antibody from Dako. It works quite well on FFPE tissue, but there is one thing that you need to be aware of and that it is not entirely specific to MTB it will also stain other gram positive bacillia. I believe that is stated on the spec sheet. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward Sent: Wednesday, May 03, 2006 8:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mycobacterium bovis (BCG) antibody My dermatopathologist asked me to look into this antibody to add to our menu to ccreen for microorganisms. I would appreciate any advice concerning vendors, conditions, etc. Thanks in advance for your help. Martha Ward Molecular Diagnostics Lab Wake Forest University Baptist Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1.1517 (20060502) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 30, Issue 3 *************************************** From kzhong888 <@t> yahoo.com Wed May 3 12:46:51 2006 From: kzhong888 <@t> yahoo.com (dfs dsaf) Date: Wed May 3 12:46:56 2006 Subject: [Histonet] Wanted cryosta, model IEC CTD harris Message-ID: <20060503174651.67287.qmail@web52901.mail.yahoo.com> Hello histonetters: I am looking for a Model CTD harris cryostat, it has been out of production since the 70's. And it is a small box looking machine often compared to a ice cream cart. if you have one for sale please email me at kzhong888@yahoo.com or call 714 675 8709. thnak you all kirk --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1¢/min. From laurie.colbert <@t> huntingtonhospital.com Wed May 3 13:03:49 2006 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed May 3 13:04:00 2006 Subject: [Histonet] Storage question Message-ID: <0BE6ADFAE4E7E04496BF21ABD346628005653B2D@EXCHANGE1.huntingtonhospital.com> We are required to label all containers. I'm sure OSHA and CAP would also require this. We order pre-filled formalin containers, so those are already appropriately labeled. We do not fill very large containers for colons, placentas, etc. Surgery and L&D have empty containers for those specimens and send them to us fresh. Then we add formalin in Pathology and put a label on the container. Laurie Colbert -----Original Message----- From: Peterson, Dan [mailto:1dpeterson@meriter.com] Sent: Wednesday, May 03, 2006 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Storage question We've recently been cited by our on-site safety committee for not having ALL of our specimen containers labeled as containing formalin. Anything we send out from the lab to outside accounts, all have a warning label. However, the specimens we collect in-house are placed into plastic containers and filled appropriately with formalin. We're talking 40-50 a day. Our staff are the only people to handle the containers from fill to disposal. I'm just curious as to how other labs are handling this. I would like to place the HCS label on the outside of the cabinets where the tissue is stored. We have formaldehyde warning placards on every door entering the lab. It just seems like a waste of time and money (for labels) for an unwarranted worry that someone can get into somewhere they don't belong and mess with something they have no idea of what they are messing with. Daniel R Peterson HT(ASCP) Histopathology Section Head General Medical Laboratories (608)-267-6557 1dpeterson@meriter.com CONFIDENTIALITY NOTICE: This message (including any attachments) is intended for the sole use of the individual and entity to whom it is addressed. This message may contain information that is confidential and is protected by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply email and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Wed May 3 13:22:06 2006 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed May 3 13:22:40 2006 Subject: [Histonet] Storage question Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C63038BE066@EMAIL.archildrens.org> It may seem like a waste of time or money but we were cited by CAP for not having our Autopsy containers labeled with warning labels. Our surgical specimens all have labels when they come from surgery. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Peterson, Dan Sent: Wednesday, May 03, 2006 12:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Storage question We've recently been cited by our on-site safety committee for not having ALL of our specimen containers labeled as containing formalin. Anything we send out from the lab to outside accounts, all have a warning label. However, the specimens we collect in-house are placed into plastic containers and filled appropriately with formalin. We're talking 40-50 a day. Our staff are the only people to handle the containers from fill to disposal. I'm just curious as to how other labs are handling this. I would like to place the HCS label on the outside of the cabinets where the tissue is stored. We have formaldehyde warning placards on every door entering the lab. It just seems like a waste of time and money (for labels) for an unwarranted worry that someone can get into somewhere they don't belong and mess with something they have no idea of what they are messing with. Daniel R Peterson HT(ASCP) Histopathology Section Head General Medical Laboratories (608)-267-6557 1dpeterson@meriter.com CONFIDENTIALITY NOTICE: This message (including any attachments) is intended for the sole use of the individual and entity to whom it is addressed. This message may contain information that is confidential and is protected by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply email and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From cwscouten <@t> myneurolab.com Wed May 3 13:28:35 2006 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Wed May 3 13:29:03 2006 Subject: [Histonet] Question Message-ID: <5784D843593D874C93E9BADCB87342AB01306EB1@tpiserver03.Coretech-holdings.com> Depends on your definition of inexpensive. Try the Equal Z at the link below: http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=4 42801&catdesc=Histology+Equipment&CatThreeID=856&CatOneID=4&subcatdesc=W hole+Organ+Sectioning+Equipment&idsubcategory=189 Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine (CDC/NCID/VR) Sent: Tuesday, May 02, 2006 1:24 PM To: Nita Searcy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Question Check with Sakura regarding their grossing tools. Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Tuesday, May 02, 2006 1:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question Anyone know of an inexpensive tool for cutting "fresh" 1 mm sections? Researcher wants to know. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cwscouten <@t> myneurolab.com Wed May 3 13:34:17 2006 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Wed May 3 13:34:45 2006 Subject: [Histonet] Question Message-ID: <5784D843593D874C93E9BADCB87342AB01306EB3@tpiserver03.Coretech-holdings.com> We have these for rodent brain. http://www.myneurolab.com/myneurolab/mnl_products_subcat.asp?idsubcatego ry=186&CatOneID=4&subcatdesc=Brain+Matrices&catdesc=Histology+Equipment Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Allen, Rhonda Sent: Tuesday, May 02, 2006 1:33 PM To: Victoria Baker; Nita Searcy; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Question There is a brain mold available from EMS. Look under Matrices and they have different kinds of matrices. Rhonda Allen BA HT(ASCP)HTL, QIHC Histotechnology Specialist II Stowers Institute 1000 E. 50th Street Kansas City, MO 64110 816-926-4305 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Tuesday, May 02, 2006 1:04 PM To: Nita Searcy; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Question The only thing I can think of is what literally looks like an "egg slicer" I think we had ordered it from MOPEC or one of the other surgical/autopsy companies some 6 years ago. It's a metal egg shaped base, and using hi-profile microtome blades you could slice fresh soft tissue (like brain)in thin even pieces. I haven't seen one in sometime, but maybe someone who reads my e-mail will know where they can be purchased these days. If I remember correctly back then it cost about $100. Good luck Vikki --- Nita Searcy wrote: > Anyone know of an inexpensive tool for cutting > "fresh" 1 mm sections? > Researcher wants to know. > > Thanks > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From stamptrain <@t> yahoo.com Wed May 3 13:54:37 2006 From: stamptrain <@t> yahoo.com (Roger Moretz) Date: Wed May 3 13:54:43 2006 Subject: [Histonet] RE: Anyone use glass knifes anymore? In-Reply-To: <16E971922EDF9A4B9E8D3216374EDD49012B7B62@wafedixm12.corp.weyer.pri> Message-ID: <20060503185437.49185.qmail@web50307.mail.yahoo.com> Are you making the standard triangular (45 degree) knives for small sections of the Ralph knives for large sections? A lot of the answer depends on what you are actually making. Roger Moretz, Ph.D. Dept of Toxiclogy Boehringer Ingelheim Pharmaceuticals Ridgefield, CT --- "Brusig, Stephanie" wrote: > I make and use glass knifes for plastic sectioning > at 3 microns. I usually have to go through a whole > glass strip to get one good knife. Does anyone make > them anymore and have the same problem? > > > ~Stephanie Brusig > > Weyerhaeuser Company > Propagation of High Value Trees > 32901 Weyerhaeuser Way S > WTC-1B10 > Federal Way, WA 98001 > 253.924.6518 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of > histonet-request@lists.utsouthwestern.edu > Sent: Wednesday, May 03, 2006 10:10 AM > To: histonet@lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 30, Issue 3 > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, > visit > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body > 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it > is more specific than "Re: Contents of Histonet > digest..." > > > Today's Topics: > > 1. CONTROL BLOCKS NEEDED (Nymeyer, Heather) > 2. Large sections (Patsy Ruegg) > 3. Autostainer tubes (Patti Loykasek) > 4. NSH Active Members (Carrie Diamond) > 5. Question (Nita Searcy) > 6. Re: Question (Victoria Baker) > 7. Re: Large sections (Gayle Callis) > 8. RE: Question (Allen, Rhonda) > 9. RE: Question (Bartlett, Jeanine (CDC/NCID/VR)) > 10. Re: Autostainer tubes (Joanne Mauger) > 11. Re: POC for Genetic Studies > (histology@gradymem.org) > 12. antibodies to eIF2 alpha and PPAR gamma (Sarka > Lhotak) > 13. IHC on frozens (Sarka Lhotak) > 14. Fwd: [Histonet] CD Abs (Linresearch@aol.com) > 15. gallocyanin stain for nissel (Elizabeth > Chlipala) > 16. RANK, ER and PR in mouse (Randolph-Habecker, > Julie) > 17. NADH staining (Yak-Nam Wang) > 18. Re: gallocyanine stain for Nissl (John > Kiernan) > 19. Inflamatory markers (CRAIG BARLOW) > 20. Re- processing fatty tissues (Malam > Jacqueline) > 21. The end of an era (McGovern, Kevin) > 22. Mycobacterium bovis (BCG) antibody (Martha > Ward) > 23. Glycerol cryoprotected tissue and cryostat > sectioning > (Kathie A Berghorn) > 24. RE: IHC on frozens (Guillermo Palao) > 25. Save These Dates For The Pennsylvania State > Histology Meeting > (Pamela Marcum) > 26. RE: Mycobacterium bovis (BCG) antibody > (Elizabeth Chlipala) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 2 May 2006 10:03:23 -0700 > From: "Nymeyer, Heather" > > Subject: [Histonet] CONTROL BLOCKS NEEDED > To: > Message-ID: > > <463911DE3F9F8D4AA3EC67632386BC62D1509E@dc1serv78.interiorhealth.ca> > Content-Type: text/plain; charset="us-ascii" > > We are in need of the following control blocks > > - Leprosy bacilli > > - Spirochete > > > > Your assistance is appreciated and thank you in > advance. > > > > Heather D. Nymeyer, RT, CEBT > > Charge Technologist, Anatomic Pathology > > Royal Inland Hospital, > > Kamloops, BC. > > 250-314-2664 > > > > > > ------------------------------ > > Message: 2 > Date: Tue, 2 May 2006 10:52:50 -0600 > From: "Patsy Ruegg" > Subject: [Histonet] Large sections > To: > Message-ID: > <200605021652.k42Gqoog014137@chip.viawest.net> > Content-Type: text/plain; charset="US-ASCII" > > Can anyone suggest a resource for getting frozen > sections of Elk Larynx? > > One of my clients asks: > "I study elk sound production. As one question we > investigate the anatomical structure of the larynx. > For this purpose I need cross sections of several > larynges (approx. 15 larynges). Cross sections at > three positions are necessary for each larynx,. The > larynges are most likely calcified at varying > degrees. I have stored the larynges frozen in saline > solution." > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech, LLC > Fitzsimmons BioScience Park > 12635 Montview Blvd. Suite 215 > Aurora, CO 80010 > P-720-859-4060 > F-720-859-4110 > wk email pruegg@ihctech.net > web site www.ihctech.net > > > This email is confidential and intended solely for > the use of the Person(s) ('the intended recipient') > to whom it was addressed. Any views or opinions > presented are solely those of the author. It may > contain information that is privileged & > confidential within the meaning of applicable law. > Accordingly any dissemination, distribution, > copying, or other use of this message, or any of its > contents, by any person other than the intended > recipient may constitute a breach of civil or > criminal law and is strictly prohibited. If you are > NOT the intended recipient please contact the sender > and dispose of this e-mail as soon as possible. > > > > > ------------------------------ > > Message: 3 > Date: Tue, 02 May 2006 10:31:31 -0700 > From: Patti Loykasek > Subject: [Histonet] Autostainer tubes > To: histonet > Message-ID: > Content-Type: text/plain; charset="US-ASCII" > > Opps - didn't put in there a supplier other than > Dako. We had a backorder problem with Dako on these. > Actually turned out to be a glitch in their system, > so all is well. Thanks for all of the good info, > though. Histotechs are a nice bunch of people! > > > Patti Loykasek BS, HTL, QIHC > PhenoPath Laboratories > Seattle, WA > > > > === message truncated === __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From benoit.delatour <@t> ibaic.u-psud.fr Wed May 3 14:01:44 2006 From: benoit.delatour <@t> ibaic.u-psud.fr (Delatour =?iso-8859-1?Q?Beno=EEt?=) Date: Wed May 3 14:02:10 2006 Subject: [Histonet] Re: Glycerol cryoprotected tissue and cryostat In-Reply-To: <200605031705.k43H5VFC008486@mail2.mail.u-psud.fr> References: <200605031705.k43H5VFC008486@mail2.mail.u-psud.fr> Message-ID: <6.0.1.1.2.20060503200429.02c69fc8@mailhost.pop.u-psud.fr> >The protocol said to clear >in glycerol which we did and the tissue is in 80% >glycerol. I have spent the morning trying to cut >this tissue on a cryostat without success. When >I put the mouse placenta in OCT using isopentane >and liquid nitrogen, the OCT freezes but the >tissue does not. Cutting it on the cryostat is >then not possible. How long does the tissue remains in glycerol? Actually glycerol is a very efficient cryoprotectant. Usually you use it at lower concentrations (10-20%) associated with dimethylsulfoxide (DMSO)to potentiate tissue penetration. 80% is very high! In anycase placing overnight tissue in a 2%DMSO - 20% glycerol solution prevents cutting the specimen with a cryostat because the temperature is too high (around -20?C) and prevents freezing of the tissue. On the other side this cryoprection method is perfect for sliding microtomy (because you can reach lowest temperature with such apparatus). What I would recommend (if compatible with your IHC protocol): -remove all glycerol by repeated baths in PBS -re-infiltrate tissue with a less potent cryoprotectant (eg sucrose) till equilibrium -cut with cryostat Benoit --------"If you think research is expensive, try disease"-------- B. Delatour Laboratoire de Neurobiologie de l'Apprentissage, de la M?moire & de la Communication, NAMC, CNRS UMR 8620, B?t 446 Universit? Paris-Sud 91405 Orsay Cedex, FRANCE Email benoit.delatour@ibaic.u-psud.fr Web http://www.namc.u-psud.fr From Amanda.Garcia <@t> TriadHospitals.com Wed May 3 14:15:29 2006 From: Amanda.Garcia <@t> TriadHospitals.com (Garcia, Amanda) Date: Wed May 3 14:15:34 2006 Subject: [Histonet] CAP inspections Message-ID: <8B63039C9DF4554C8FDBF31235F0E1480150D49D@CPRTEVS02.triadhospitals.net> Has anyone recently gone through their CAP "unannounced" inspection and if so, how did it differ from the way inspections were previously conducted. ( if there were any differences other than being unannounced) Thanks for anyone willing to share their experience. > Amanda (Amy) Garcia > Histology/Pathology > College Station Medical Center > (979) 680-5372 office > (979) 696-5446 fax > *Mailto:amanda.garcia@triadhospitals.com > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the e-mail or any > of its attachments, please be advised that you have received this > e-mail in error and that any use, dissemination, distribution, > forwarding, printing, or copying of this e-mail or any attached files > is strictly prohibited. If you have received this e-mail in error, > please immediately purge it and all attachments and notify the sender > by reply e-mail or contact the sender at the number listed. > > > From sluhisto <@t> yahoo.com Wed May 3 14:31:11 2006 From: sluhisto <@t> yahoo.com (Histology SLU) Date: Wed May 3 14:31:16 2006 Subject: [Histonet] CAP inspections In-Reply-To: <8B63039C9DF4554C8FDBF31235F0E1480150D49D@CPRTEVS02.triadhospitals.net> Message-ID: <20060503193111.58193.qmail@web51007.mail.yahoo.com> I would be very interested in this information as well. Please post to the group. Thanks to all and hope, if you have been through this new process, it went well. Susan "Garcia, Amanda" wrote: Has anyone recently gone through their CAP "unannounced" inspection and if so, how did it differ from the way inspections were previously conducted. ( if there were any differences other than being unannounced) Thanks for anyone willing to share their experience. > Amanda (Amy) Garcia > Histology/Pathology > College Station Medical Center > (979) 680-5372 office > (979) 696-5446 fax > *Mailto:amanda.garcia@triadhospitals.com > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the e-mail or any > of its attachments, please be advised that you have received this > e-mail in error and that any use, dissemination, distribution, > forwarding, printing, or copying of this e-mail or any attached files > is strictly prohibited. If you have received this e-mail in error, > please immediately purge it and all attachments and notify the sender > by reply e-mail or contact the sender at the number listed. > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- New Yahoo! Messenger with Voice. Call regular phones from your PC and save big. From gayle.brosnanwatters <@t> sru.edu Wed May 3 14:39:19 2006 From: gayle.brosnanwatters <@t> sru.edu (gayle brosnanwatters) Date: Wed May 3 14:39:26 2006 Subject: [Histonet] RE: Anyone use glass knifes anymore? Message-ID: <8172F14C39FCAF4E99E266F6756A3683074B115E@msfexch01.srunet.sruad.edu> I use glass knives (triangles) that I make from 1/2 inch glass on an ancient knife maker that I play heck keeping operating. I, too, am frustrated by how hard it is to make decent knives. I section plastic embedded mouse brains at between 1 and 3 microns and do light microscopy. Even when my knives are working well, I can expect to use two or three per mouse brain, in order to get the sections I want. My glass pieces are not the long strips of 1/4 inch pieces (although I have used them), but shorter rectangles. I think if you can get your knife maker working well, it can make a difference. I just had mine sent off to Energy Beam Sciences, and they got it working, but then they shipped it back to me and I really think that the shipper dropped it on the way, because it has not been working well since. I understand your frustration, although I don't have an answer. I wondered if the fact that my glass is old would make a difference. I know just enough chemistry to remember that glass is a liquid that does change form as it ages, and this glass was given to me and was old then. Does anyone know if that would affect it? Gayle L. Brosnan-Watters, PhD Assistant Professor Dept of Psychology Treasurer, Faculty for Undergraduate Neuroscience 226 Vincent Science Hall Slippery Rock University Slippery Rock, PA 16057 gayle.brosnanwatters@sru.edu 724-738-2529 - office 724-738-4807 - fax > I make and use glass knifes for plastic sectioning > at 3 microns. I usually have to go through a whole > glass strip to get one good knife. Does anyone make > them anymore and have the same problem? > > > ~Stephanie Brusig > > Weyerhaeuser Company > Propagation of High Value Trees > 32901 Weyerhaeuser Way S > WTC-1B10 > Federal Way, WA 98001 > 253.924.6518 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of > histonet-request@lists.utsouthwestern.edu > Sent: Wednesday, May 03, 2006 10:10 AM > To: histonet@lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 30, Issue 3 > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, > visit > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body > 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it > is more specific than "Re: Contents of Histonet > digest..." > > > Today's Topics: > > 1. CONTROL BLOCKS NEEDED (Nymeyer, Heather) > 2. Large sections (Patsy Ruegg) > 3. Autostainer tubes (Patti Loykasek) > 4. NSH Active Members (Carrie Diamond) > 5. Question (Nita Searcy) > 6. Re: Question (Victoria Baker) > 7. Re: Large sections (Gayle Callis) > 8. RE: Question (Allen, Rhonda) > 9. RE: Question (Bartlett, Jeanine (CDC/NCID/VR)) > 10. Re: Autostainer tubes (Joanne Mauger) > 11. Re: POC for Genetic Studies > (histology@gradymem.org) > 12. antibodies to eIF2 alpha and PPAR gamma (Sarka > Lhotak) > 13. IHC on frozens (Sarka Lhotak) > 14. Fwd: [Histonet] CD Abs (Linresearch@aol.com) > 15. gallocyanin stain for nissel (Elizabeth > Chlipala) > 16. RANK, ER and PR in mouse (Randolph-Habecker, > Julie) > 17. NADH staining (Yak-Nam Wang) > 18. Re: gallocyanine stain for Nissl (John > Kiernan) > 19. Inflamatory markers (CRAIG BARLOW) > 20. Re- processing fatty tissues (Malam > Jacqueline) > 21. The end of an era (McGovern, Kevin) > 22. Mycobacterium bovis (BCG) antibody (Martha > Ward) > 23. Glycerol cryoprotected tissue and cryostat > sectioning > (Kathie A Berghorn) > 24. RE: IHC on frozens (Guillermo Palao) > 25. Save These Dates For The Pennsylvania State > Histology Meeting > (Pamela Marcum) > 26. RE: Mycobacterium bovis (BCG) antibody > (Elizabeth Chlipala) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 2 May 2006 10:03:23 -0700 > From: "Nymeyer, Heather" > > Subject: [Histonet] CONTROL BLOCKS NEEDED > To: > Message-ID: > > <463911DE3F9F8D4AA3EC67632386BC62D1509E@dc1serv78.interiorhealth.ca> > Content-Type: text/plain; charset="us-ascii" > > We are in need of the following control blocks > > - Leprosy bacilli > > - Spirochete > > > > Your assistance is appreciated and thank you in > advance. > > > > Heather D. Nymeyer, RT, CEBT > > Charge Technologist, Anatomic Pathology > > Royal Inland Hospital, > > Kamloops, BC. > > 250-314-2664 > > > > > > ------------------------------ > > Message: 2 > Date: Tue, 2 May 2006 10:52:50 -0600 > From: "Patsy Ruegg" > Subject: [Histonet] Large sections > To: > Message-ID: > <200605021652.k42Gqoog014137@chip.viawest.net> > Content-Type: text/plain; charset="US-ASCII" > > Can anyone suggest a resource for getting frozen > sections of Elk Larynx? > > One of my clients asks: > "I study elk sound production. As one question we > investigate the anatomical structure of the larynx. > For this purpose I need cross sections of several > larynges (approx. 15 larynges). Cross sections at > three positions are necessary for each larynx,. The > larynges are most likely calcified at varying > degrees. I have stored the larynges frozen in saline > solution." > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech, LLC > Fitzsimmons BioScience Park > 12635 Montview Blvd. Suite 215 > Aurora, CO 80010 > P-720-859-4060 > F-720-859-4110 > wk email pruegg@ihctech.net > web site www.ihctech.net > > > This email is confidential and intended solely for > the use of the Person(s) ('the intended recipient') > to whom it was addressed. Any views or opinions > presented are solely those of the author. It may > contain information that is privileged & > confidential within the meaning of applicable law. > Accordingly any dissemination, distribution, > copying, or other use of this message, or any of its > contents, by any person other than the intended > recipient may constitute a breach of civil or > criminal law and is strictly prohibited. If you are > NOT the intended recipient please contact the sender > and dispose of this e-mail as soon as possible. > > > > > ------------------------------ > > Message: 3 > Date: Tue, 02 May 2006 10:31:31 -0700 > From: Patti Loykasek > Subject: [Histonet] Autostainer tubes > To: histonet > Message-ID: > Content-Type: text/plain; charset="US-ASCII" > > Opps - didn't put in there a supplier other than > Dako. We had a backorder problem with Dako on these. > Actually turned out to be a glitch in their system, > so all is well. Thanks for all of the good info, > though. Histotechs are a nice bunch of people! > > > Patti Loykasek BS, HTL, QIHC > PhenoPath Laboratories > Seattle, WA > > > > === message truncated === __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Wed May 3 14:53:35 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed May 3 14:53:49 2006 Subject: [Histonet] Accidentally frozen NBF fixed tissue In-Reply-To: References: Message-ID: <6.0.0.22.1.20060503134655.01b8f4a0@gemini.msu.montana.edu> Nora, Only when the NBF tissues were cryoprotected with sucrose before snap freezeing and never at -80C, always much colder. There is a wonderful article in Histologic by Rena Fail and Vinnie Della Speranza on recovering poorly snap frozen biopsies where the ice crystal formation is obvious. It was written last year or so. Go to Sakura Finetek website,, click on Histologic and see how they did this. It was easy and with good results on getting a decent section. At 11:23 AM 5/3/2006, you wrote: >Hello all, > >Someone in our lab accidentally froze 10% NBF fixed pancreas at -80 >degree C. >They now want to get slides for H&E etc off that. Does anyone know >whether this tissue is still usable after being frozen? >Obviously it is not good to freeze fixed tissue, but has anyone ever >done that and still gotten usable results? I would appreciate any input! Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From Jackie.O'Connor <@t> abbott.com Wed May 3 14:56:05 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed May 3 14:56:31 2006 Subject: [Histonet] Storage question In-Reply-To: <328CBAE62F31C642B422970E879DFADC01A80089@pcwex01> Message-ID: Let's pretend ONE container is being transported from 'here to there' and it gets dropped off a cart in the hallway - but no one notices. Joe Janitor comes along and finds the leaking container and tries to clean up the spill - of course he has no idea what it is because the container isn't labeled and he doesn't know anything about pathology. The liquid (formalin) splashes in his eye - he runs to the ER in pain, but has no idea what splashed in his eye. That's the type of scenario your safety people are trying to prevent. I know there are guidelines in place with one or more federal agencies dictating that all hazardous containers must be labeled with appropriate hazard warnings. "Peterson, Dan" <1dpeterson@meriter.com> Sent by: histonet-bounces@lists.utsouthwestern.edu 05/03/2006 12:42 PM To cc Subject [Histonet] Storage question We've recently been cited by our on-site safety committee for not having ALL of our specimen containers labeled as containing formalin. Anything we send out from the lab to outside accounts, all have a warning label. However, the specimens we collect in-house are placed into plastic containers and filled appropriately with formalin. We're talking 40-50 a day. Our staff are the only people to handle the containers from fill to disposal. I'm just curious as to how other labs are handling this. I would like to place the HCS label on the outside of the cabinets where the tissue is stored. We have formaldehyde warning placards on every door entering the lab. It just seems like a waste of time and money (for labels) for an unwarranted worry that someone can get into somewhere they don't belong and mess with something they have no idea of what they are messing with. Daniel R Peterson HT(ASCP) Histopathology Section Head General Medical Laboratories (608)-267-6557 1dpeterson@meriter.com CONFIDENTIALITY NOTICE: This message (including any attachments) is intended for the sole use of the individual and entity to whom it is addressed. This message may contain information that is confidential and is protected by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply email and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lyonm <@t> upstate.edu Wed May 3 15:02:02 2006 From: lyonm <@t> upstate.edu (Michael J. Lyon, Ph.D.) Date: Wed May 3 15:01:47 2006 Subject: [Histonet] Large sections In-Reply-To: <200605021652.k42Gqoog014137@chip.viawest.net> Message-ID: <004501c66eec$71eb2190$31ae7f8b@lyonoffice> Patsy: We have in the past done serial sections of human larynges, both cross and longitudinal. They were formalin fixed, decalcified in formic acid and paraffin embedded. I would say the there would be some structural damage due to the freezing in saline and it would be tough to get good frozen sections since the thyroid cartilage may be calcified as it is in humans. What is your client truly after??? Mike Michael J. Lyon, Ph.D. Associate Prof Otolaryngology SUNY Upstate Med. Univ. > -----Original Message----- > From: Patsy Ruegg [mailto:pruegg@ihctech.net] > Sent: Tuesday, May 02, 2006 12:53 PM > To: histonet@pathology.swmed.edu > Subject: [Histonet] Large sections > > Can anyone suggest a resource for getting frozen sections of Elk Larynx? > > One of my clients asks: > "I study elk sound production. As one question we investigate the > anatomical > structure of the larynx. For this purpose I need cross sections of several > larynges (approx. 15 larynges). Cross sections at three positions are > necessary for each larynx,. The larynges are most likely calcified at > varying degrees. I have stored the larynges frozen in saline solution." > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech, LLC > Fitzsimmons BioScience Park > 12635 Montview Blvd. Suite 215 > Aurora, CO 80010 > P-720-859-4060 > F-720-859-4110 > wk email pruegg@ihctech.net > web site www.ihctech.net > > > This email is confidential and intended solely for the use of the > Person(s) > ('the intended recipient') to whom it was addressed. Any views or opinions > presented are solely those of the author. It may contain information that > is > privileged & confidential within the meaning of applicable law. > Accordingly > any dissemination, distribution, copying, or other use of this message, or > any of its contents, by any person other than the intended recipient may > constitute a breach of civil or criminal law and is strictly prohibited. > If > you are NOT the intended recipient please contact the sender and dispose > of > this e-mail as soon as possible. > > From stamptrain <@t> yahoo.com Wed May 3 18:37:41 2006 From: stamptrain <@t> yahoo.com (Roger Moretz) Date: Wed May 3 18:37:44 2006 Subject: [Histonet] RE: Anyone use glass knifes anymore? In-Reply-To: <8172F14C39FCAF4E99E266F6756A3683074B115E@msfexch01.srunet.sruad.edu> Message-ID: <20060503233741.62619.qmail@web50304.mail.yahoo.com> Stephanie U Gayle: The old triangle glass knife makers (LKB, EBS, DuPont) have a number of mechanical issues that need to be addressed by the user--especially if you are having a low percentage of good knives. First, when was the last time you changed the scoring wheel? That would be my first step. Second, would be the damping pads and it probably wouldn't hurt to replace them. Next, go through a check of the procedure following the manual to evaluate the scoring pressure (follow the user's manual as you do this). Next, check the length of the score--is it as described by the manual? Finally, (and this is a very personal thing) I use a slow break method (i.e. turn the pressure lever/wheel) just until you see the glass begin to fracture, then let it fracture on its own. I have had several LKB, Leica and Dupont knifemakers over the years. These procedures have always results in a high percentage of good knives. Oh yes. Be very careful when you pick up the knives--if they touch at that point, then they're probably no good! Roger Moretz, Ph.D. Dept of Toxicology Boehringer Ingelheim Pharmaceuticals Ridgefield CT --- gayle brosnanwatters wrote: > I use glass knives (triangles) that I make from 1/2 > inch glass on an > ancient knife maker that I play heck keeping > operating. I, too, am > frustrated by how hard it is to make decent knives. > I section plastic > embedded mouse brains at between 1 and 3 microns and > do light > microscopy. Even when my knives are working well, I > can expect to use > two or three per mouse brain, in order to get the > sections I want. My > glass pieces are not the long strips of 1/4 inch > pieces (although I have > used them), but shorter rectangles. I think if you > can get your knife > maker working well, it can make a difference. I > just had mine sent off > to Energy Beam Sciences, and they got it working, > but then they shipped > it back to me and I really think that the shipper > dropped it on the way, > because it has not been working well since. I > understand your > frustration, although I don't have an answer. I > wondered if the fact > that my glass is old would make a difference. I > know just enough > chemistry to remember that glass is a liquid that > does change form as it > ages, and this glass was given to me and was old > then. Does anyone know > if that would affect it? > Gayle L. Brosnan-Watters, PhD > Assistant Professor > Dept of Psychology > Treasurer, Faculty for Undergraduate Neuroscience > 226 Vincent Science Hall > Slippery Rock University > Slippery Rock, PA 16057 > gayle.brosnanwatters@sru.edu > 724-738-2529 - office > 724-738-4807 - fax > > > I make and use glass knifes for plastic > sectioning > > at 3 microns. I usually have to go through a whole > > glass strip to get one good knife. Does anyone > make > > them anymore and have the same problem? > > > > > > ~Stephanie Brusig > > > > Weyerhaeuser Company > > Propagation of High Value Trees > > 32901 Weyerhaeuser Way S > > WTC-1B10 > > Federal Way, WA 98001 > > 253.924.6518 > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] > > On Behalf Of > > histonet-request@lists.utsouthwestern.edu > > Sent: Wednesday, May 03, 2006 10:10 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: Histonet Digest, Vol 30, Issue 3 > > > > Send Histonet mailing list submissions to > > histonet@lists.utsouthwestern.edu > > > > To subscribe or unsubscribe via the World Wide > Web, > > visit > > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > or, via email, send a message with subject or body > > 'help' to > > histonet-request@lists.utsouthwestern.edu > > > > You can reach the person managing the list at > > histonet-owner@lists.utsouthwestern.edu > > > > When replying, please edit your Subject line so it > > is more specific than "Re: Contents of Histonet > > digest..." > > > > > > Today's Topics: > > > > 1. CONTROL BLOCKS NEEDED (Nymeyer, Heather) > > 2. Large sections (Patsy Ruegg) > > 3. Autostainer tubes (Patti Loykasek) > > 4. NSH Active Members (Carrie Diamond) > > 5. Question (Nita Searcy) > > 6. Re: Question (Victoria Baker) > > 7. Re: Large sections (Gayle Callis) > > 8. RE: Question (Allen, Rhonda) > > 9. RE: Question (Bartlett, Jeanine > (CDC/NCID/VR)) > > 10. Re: Autostainer tubes (Joanne Mauger) > > 11. Re: POC for Genetic Studies > > (histology@gradymem.org) > > 12. antibodies to eIF2 alpha and PPAR gamma > (Sarka > > Lhotak) > > 13. IHC on frozens (Sarka Lhotak) > > 14. Fwd: [Histonet] CD Abs (Linresearch@aol.com) > > 15. gallocyanin stain for nissel (Elizabeth > > Chlipala) > > 16. RANK, ER and PR in mouse (Randolph-Habecker, > > Julie) > > 17. NADH staining (Yak-Nam Wang) > > 18. Re: gallocyanine stain for Nissl (John > > Kiernan) > > 19. Inflamatory markers (CRAIG BARLOW) > > 20. Re- processing fatty tissues (Malam > > Jacqueline) > > 21. The end of an era (McGovern, Kevin) > > 22. Mycobacterium bovis (BCG) antibody (Martha > > Ward) > > 23. Glycerol cryoprotected tissue and cryostat > > sectioning > > (Kathie A Berghorn) > > 24. RE: IHC on frozens (Guillermo Palao) > > 25. Save These Dates For The Pennsylvania State > > Histology Meeting > > (Pamela Marcum) > > 26. RE: Mycobacterium bovis (BCG) antibody > > (Elizabeth Chlipala) > > > > > > > ---------------------------------------------------------------------- > > > > Message: 1 > > Date: Tue, 2 May 2006 10:03:23 -0700 > > From: "Nymeyer, Heather" > > > > Subject: [Histonet] CONTROL BLOCKS NEEDED > > To: > > Message-ID: > > > > > <463911DE3F9F8D4AA3EC67632386BC62D1509E@dc1serv78.interiorhealth.ca> > > Content-Type: text/plain; charset="us-ascii" > > > > We are in need of the following control blocks > > > > - Leprosy bacilli > > > > - Spirochete > > > > > > > > Your assistance is appreciated and thank you in > > advance. > > > > > > > > Heather D. Nymeyer, RT, CEBT > > > > Charge Technologist, Anatomic Pathology > > > > Royal Inland Hospital, > > > > Kamloops, BC. > > > > 250-314-2664 > > > > > > > > > > > > ------------------------------ > > > > Message: 2 > > Date: Tue, 2 May 2006 10:52:50 -0600 > > From: "Patsy Ruegg" > > Subject: [Histonet] Large sections > > To: > > Message-ID: > > <200605021652.k42Gqoog014137@chip.viawest.net> > > Content-Type: text/plain; charset="US-ASCII" > > > > Can anyone suggest a resource for getting frozen > > sections of Elk Larynx? > > > > One of my clients asks: > > "I study elk sound production. As one question we > > investigate the anatomical structure of the > larynx. > > For this purpose I need cross sections of several > > larynges (approx. 15 larynges). Cross sections at > > three positions are necessary for each larynx,. > The > > larynges are most likely calcified at varying > === message truncated === __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From eboyden3 <@t> gmail.com Wed May 3 18:38:20 2006 From: eboyden3 <@t> gmail.com (Ed Boyden) Date: Wed May 3 18:38:25 2006 Subject: [Histonet] Protocols for estimating the swelling of tissue? Message-ID: Many procedures (fixation, immersion in sucrose, etc.) cause swelling of tissue, versus the intact, original tissue. Does anyone know of either: 1. ways to predict the amount of swelling that occurs with a specific protocol, or 2. a protcol that would allow swelling of the tissue by a predictable amount? I would like to be able to compare samples done at different times, and stored in different ways. If not possible, I would like to be able to prepare new samples so that their swelling will match my old estimates, as much as possible, or at least that I can calculate the swelling of the new samples. Thanks so much for your help! Best, Ed From louise.renton <@t> gmail.com Thu May 4 01:51:49 2006 From: louise.renton <@t> gmail.com (louise renton) Date: Thu May 4 01:59:10 2006 Subject: [Histonet] RE: Anyone use glass knifes anymore? In-Reply-To: <8172F14C39FCAF4E99E266F6756A3683074B115E@msfexch01.srunet.sruad.edu> References: <8172F14C39FCAF4E99E266F6756A3683074B115E@msfexch01.srunet.sruad.edu> Message-ID: In BIll Bryson's "A brief history of nearly everything" he states that although glass is in fact a supercooled liquid, the amount of change in sheets of glass, even after hundreds of years is so small as to be unmeasurable by normal means. I'd lay my bets on the knife breaker On 5/3/06, gayle brosnanwatters wrote: > I use glass knives (triangles) that I make from 1/2 inch glass on an > ancient knife maker that I play heck keeping operating. I, too, am > frustrated by how hard it is to make decent knives. I section plastic > embedded mouse brains at between 1 and 3 microns and do light > microscopy. Even when my knives are working well, I can expect to use > two or three per mouse brain, in order to get the sections I want. My > glass pieces are not the long strips of 1/4 inch pieces (although I have > used them), but shorter rectangles. I think if you can get your knife > maker working well, it can make a difference. I just had mine sent off > to Energy Beam Sciences, and they got it working, but then they shipped > it back to me and I really think that the shipper dropped it on the way, > because it has not been working well since. I understand your > frustration, although I don't have an answer. I wondered if the fact > that my glass is old would make a difference. I know just enough > chemistry to remember that glass is a liquid that does change form as it > ages, and this glass was given to me and was old then. Does anyone know > if that would affect it? > Gayle L. Brosnan-Watters, PhD > Assistant Professor > Dept of Psychology > Treasurer, Faculty for Undergraduate Neuroscience > 226 Vincent Science Hall > Slippery Rock University > Slippery Rock, PA 16057 > gayle.brosnanwatters@sru.edu > 724-738-2529 - office > 724-738-4807 - fax > > > I make and use glass knifes for plastic sectioning > > at 3 microns. I usually have to go through a whole > > glass strip to get one good knife. Does anyone make > > them anymore and have the same problem? > > > > > > ~Stephanie Brusig > > > > Weyerhaeuser Company > > Propagation of High Value Trees > > 32901 Weyerhaeuser Way S > > WTC-1B10 > > Federal Way, WA 98001 > > 253.924.6518 > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] > > On Behalf Of > > histonet-request@lists.utsouthwestern.edu > > Sent: Wednesday, May 03, 2006 10:10 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: Histonet Digest, Vol 30, Issue 3 > > > > Send Histonet mailing list submissions to > > histonet@lists.utsouthwestern.edu > > > > To subscribe or unsubscribe via the World Wide Web, > > visit > > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > or, via email, send a message with subject or body > > 'help' to > > histonet-request@lists.utsouthwestern.edu > > > > You can reach the person managing the list at > > histonet-owner@lists.utsouthwestern.edu > > > > When replying, please edit your Subject line so it > > is more specific than "Re: Contents of Histonet > > digest..." > > > > > > Today's Topics: > > > > 1. CONTROL BLOCKS NEEDED (Nymeyer, Heather) > > 2. Large sections (Patsy Ruegg) > > 3. Autostainer tubes (Patti Loykasek) > > 4. NSH Active Members (Carrie Diamond) > > 5. Question (Nita Searcy) > > 6. Re: Question (Victoria Baker) > > 7. Re: Large sections (Gayle Callis) > > 8. RE: Question (Allen, Rhonda) > > 9. RE: Question (Bartlett, Jeanine (CDC/NCID/VR)) > > 10. Re: Autostainer tubes (Joanne Mauger) > > 11. Re: POC for Genetic Studies > > (histology@gradymem.org) > > 12. antibodies to eIF2 alpha and PPAR gamma (Sarka > > Lhotak) > > 13. IHC on frozens (Sarka Lhotak) > > 14. Fwd: [Histonet] CD Abs (Linresearch@aol.com) > > 15. gallocyanin stain for nissel (Elizabeth > > Chlipala) > > 16. RANK, ER and PR in mouse (Randolph-Habecker, > > Julie) > > 17. NADH staining (Yak-Nam Wang) > > 18. Re: gallocyanine stain for Nissl (John > > Kiernan) > > 19. Inflamatory markers (CRAIG BARLOW) > > 20. Re- processing fatty tissues (Malam > > Jacqueline) > > 21. The end of an era (McGovern, Kevin) > > 22. Mycobacterium bovis (BCG) antibody (Martha > > Ward) > > 23. Glycerol cryoprotected tissue and cryostat > > sectioning > > (Kathie A Berghorn) > > 24. RE: IHC on frozens (Guillermo Palao) > > 25. Save These Dates For The Pennsylvania State > > Histology Meeting > > (Pamela Marcum) > > 26. RE: Mycobacterium bovis (BCG) antibody > > (Elizabeth Chlipala) > > > > > > > ---------------------------------------------------------------------- > > > > Message: 1 > > Date: Tue, 2 May 2006 10:03:23 -0700 > > From: "Nymeyer, Heather" > > > > Subject: [Histonet] CONTROL BLOCKS NEEDED > > To: > > Message-ID: > > > > > <463911DE3F9F8D4AA3EC67632386BC62D1509E@dc1serv78.interiorhealth.ca> > > Content-Type: text/plain; charset="us-ascii" > > > > We are in need of the following control blocks > > > > - Leprosy bacilli > > > > - Spirochete > > > > > > > > Your assistance is appreciated and thank you in > > advance. > > > > > > > > Heather D. Nymeyer, RT, CEBT > > > > Charge Technologist, Anatomic Pathology > > > > Royal Inland Hospital, > > > > Kamloops, BC. > > > > 250-314-2664 > > > > > > > > > > > > ------------------------------ > > > > Message: 2 > > Date: Tue, 2 May 2006 10:52:50 -0600 > > From: "Patsy Ruegg" > > Subject: [Histonet] Large sections > > To: > > Message-ID: > > <200605021652.k42Gqoog014137@chip.viawest.net> > > Content-Type: text/plain; charset="US-ASCII" > > > > Can anyone suggest a resource for getting frozen > > sections of Elk Larynx? > > > > One of my clients asks: > > "I study elk sound production. As one question we > > investigate the anatomical structure of the larynx. > > For this purpose I need cross sections of several > > larynges (approx. 15 larynges). Cross sections at > > three positions are necessary for each larynx,. The > > larynges are most likely calcified at varying > > degrees. I have stored the larynges frozen in saline > > solution." > > > > Patsy Ruegg, HT(ASCP)QIHC > > IHCtech, LLC > > Fitzsimmons BioScience Park > > 12635 Montview Blvd. Suite 215 > > Aurora, CO 80010 > > P-720-859-4060 > > F-720-859-4110 > > wk email pruegg@ihctech.net > > web site www.ihctech.net > > > > > > This email is confidential and intended solely for > > the use of the Person(s) ('the intended recipient') > > to whom it was addressed. Any views or opinions > > presented are solely those of the author. It may > > contain information that is privileged & > > confidential within the meaning of applicable law. > > Accordingly any dissemination, distribution, > > copying, or other use of this message, or any of its > > contents, by any person other than the intended > > recipient may constitute a breach of civil or > > criminal law and is strictly prohibited. If you are > > NOT the intended recipient please contact the sender > > and dispose of this e-mail as soon as possible. > > > > > > > > > > ------------------------------ > > > > Message: 3 > > Date: Tue, 02 May 2006 10:31:31 -0700 > > From: Patti Loykasek > > Subject: [Histonet] Autostainer tubes > > To: histonet > > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > > > Opps - didn't put in there a supplier other than > > Dako. We had a backorder problem with Dako on these. > > Actually turned out to be a glitch in their system, > > so all is well. Thanks for all of the good info, > > though. Histotechs are a nice bunch of people! > > > > > > Patti Loykasek BS, HTL, QIHC > > PhenoPath Laboratories > > Seattle, WA > > > > > > > > > === message truncated === > > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "Does a conceited cowboy, only ride on pompous grass?. From kemlo.rogerson <@t> waht.swest.nhs.uk Thu May 4 02:03:35 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu May 4 02:02:31 2006 Subject: [Histonet] Protocols for estimating the swelling of tissue? Message-ID: I thought, and I stand to be corrected, that most fixatives caused shrinkage of tissue. I assumed that to be due to the fixation, condensation, cross linking of proteins or the eradication of water molecules. Hypertonic sucrose would also cause shrinkage too as it would exert a negative osmotic pressure on the 'more dilute' tissue, wouldn't it or have I got my pressures all wrong. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 One's action ought to come out of an achieved stillness: not to be a mere rushing on. -- D.H. Lawrence This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Ed Boyden [mailto:eboyden3@gmail.com] Sent: Thursday, May 04, 2006 12:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Protocols for estimating the swelling of tissue? Many procedures (fixation, immersion in sucrose, etc.) cause swelling of tissue, versus the intact, original tissue. Does anyone know of either: 1. ways to predict the amount of swelling that occurs with a specific protocol, or 2. a protcol that would allow swelling of the tissue by a predictable amount? I would like to be able to compare samples done at different times, and stored in different ways. If not possible, I would like to be able to prepare new samples so that their swelling will match my old estimates, as much as possible, or at least that I can calculate the swelling of the new samples. Thanks so much for your help! Best, Ed _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo.rogerson <@t> waht.swest.nhs.uk Thu May 4 02:11:32 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu May 4 02:10:20 2006 Subject: [Histonet] Storage question Message-ID: My you Yanks are really years behind with safety. Do you understand COSHH or Health and Safety at work? In the UK each and every specimen container that contains anything must have the identity of what is in it and what hazard is poses. In the case of formalin it cites that it is harmful, that it is formalin and, together with a whole load of other info, what to use in case of it getting into eyes etc. In the land of litigation I amazed you rely on people not messing with something because they don't know what it is. Surely people need to know what do if they are accidentally exposed to something they shouldn't be messing with even if they tried to commit suicide with it then decided they oughtn't to bother. Do you have spillage kits for formalin and SOPs for Porters and Drivers who transport these samples just in case they have an accident? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 One's action ought to come out of an achieved stillness: not to be a mere rushing on. -- D.H. Lawrence This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Peterson, Dan [mailto:1dpeterson@meriter.com] Sent: Wednesday, May 03, 2006 6:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Storage question We've recently been cited by our on-site safety committee for not having ALL of our specimen containers labeled as containing formalin. Anything we send out from the lab to outside accounts, all have a warning label. However, the specimens we collect in-house are placed into plastic containers and filled appropriately with formalin. We're talking 40-50 a day. Our staff are the only people to handle the containers from fill to disposal. I'm just curious as to how other labs are handling this. I would like to place the HCS label on the outside of the cabinets where the tissue is stored. We have formaldehyde warning placards on every door entering the lab. It just seems like a waste of time and money (for labels) for an unwarranted worry that someone can get into somewhere they don't belong and mess with something they have no idea of what they are messing with. Daniel R Peterson HT(ASCP) Histopathology Section Head General Medical Laboratories (608)-267-6557 1dpeterson@meriter.com CONFIDENTIALITY NOTICE: This message (including any attachments) is intended for the sole use of the individual and entity to whom it is addressed. This message may contain information that is confidential and is protected by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply email and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JGREWE <@t> OhioHealth.com Thu May 4 05:07:29 2006 From: JGREWE <@t> OhioHealth.com (JGREWE@OhioHealth.com) Date: Thu May 4 05:07:38 2006 Subject: [Histonet] Milestone Feedback for Douglas Deltour Message-ID: We tested tissue for several weeks using the RHS-1. Our pathologists liked the results so much that we have purchased 3 Pathos units and are expecting delivery in about a month. I can give you better feedback after we are up and running. Please feel free to call. Jackie Grewe, Histology Supervisor Riverside Methodist Hospital 3535 Olentangy River Road Columbus, Ohio 43214 614-566-5679 From MDiCarlo <@t> KaleidaHealth.Org Thu May 4 07:13:33 2006 From: MDiCarlo <@t> KaleidaHealth.Org (DiCarlo, Margaret) Date: Thu May 4 07:13:41 2006 Subject: [Histonet] paraffin scraper Message-ID: <9B4A77DF11463E4FB723D484214AE9BC286C1D@KALEXMB02.KaleidaHealth.org> Hello everyone, I am looking to purchase a very long handled paraffin scraper that you attach a razor blade at the end. This is so I can stand and scrape instead of squatting and using a putty knife. I checked the Fisher and VWR catalogs and was unsucessful. I have called at least two Home Depot stores and they don't sell them either. Can you direct me to some company or place where I can buy this ? I will appreciate your assistance. Peggy DiCarlo HT (ASCP) Orthopedics Bone Lab Buffalo General Hospital 100 High St. Buffalo, NY 14203 716-859-1293 _____ Upgrade Your Email - Click here! CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From jnocito <@t> pathreflab.com Thu May 4 07:16:20 2006 From: jnocito <@t> pathreflab.com (Joe Nocito) Date: Thu May 4 07:13:44 2006 Subject: [Histonet] Storage question In-Reply-To: <328CBAE62F31C642B422970E879DFADC01A80089@pcwex01> Message-ID: Sorry Daniel, We purchase pre-filled formalin containers with the labels already attached. With so much litigation today, why take a chance? Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Peterson, Dan Sent: Wednesday, May 03, 2006 12:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Storage question We've recently been cited by our on-site safety committee for not having ALL of our specimen containers labeled as containing formalin. Anything we send out from the lab to outside accounts, all have a warning label. However, the specimens we collect in-house are placed into plastic containers and filled appropriately with formalin. We're talking 40-50 a day. Our staff are the only people to handle the containers from fill to disposal. I'm just curious as to how other labs are handling this. I would like to place the HCS label on the outside of the cabinets where the tissue is stored. We have formaldehyde warning placards on every door entering the lab. It just seems like a waste of time and money (for labels) for an unwarranted worry that someone can get into somewhere they don't belong and mess with something they have no idea of what they are messing with. Daniel R Peterson HT(ASCP) Histopathology Section Head General Medical Laboratories (608)-267-6557 1dpeterson@meriter.com CONFIDENTIALITY NOTICE: This message (including any attachments) is intended for the sole use of the individual and entity to whom it is addressed. This message may contain information that is confidential and is protected by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply email and then delete the message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kmerriam2003 <@t> yahoo.com Thu May 4 07:20:27 2006 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Thu May 4 07:20:40 2006 Subject: [Histonet] Accidentally frozen NBF fixed tissue In-Reply-To: Message-ID: <20060504122027.54746.qmail@web50307.mail.yahoo.com> Nora, A couple of years ago, one of the researchers sent me some OCT pancreas samples in dry ice and, to save time, also put the NBF-fixed pancreas' in the same container. Needless to say that the formalin (and the tissues) were frozen solid when they got here. I processed them, did H&Es and a bunch of IHC's on them. They were fine, the artifact was minimal. Here's another interesting story. One of the researchers decided that he wanted some histology on some Zucker rat livers that had been snap-frozen in liquid nitrogen and stored at -80 for a year. I thought that we might be able to salvage the samples, so I took livers and placed them into cold formalin and they "puffed-up" within about an hour. I let them fix overnight at room temperature, trimmed them down to size, let them fix another day and then processed as usual. I did H&Es and Trichromes on them. They did not look too bad, the sinusoidal spaces were wider than normal, but since the pathologist was aware of what had happened to the tissue, he was able to get some good data out if the study. Good luck with your samples, Kim Nora Berghoff wrote: Hello all, Someone in our lab accidentally froze 10% NBF fixed pancreas at -80 degree C. They now want to get slides for H&E etc off that. Does anyone know whether this tissue is still usable after being frozen? Obviously it is not good to freeze fixed tissue, but has anyone ever done that and still gotten usable results? I would appreciate any input! Thanks so much, Nora Berghoff ************************************** Nora Berghoff, med. vet. Research Assistant Gastrointestinal Laboratory College of Veterinary Medicine Texas A&M University Phone: (979) 458 2293 (w) ************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Kim Merriam, MA, HT(ASCP) Novartis Cambridge, MA --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. From PMonfils <@t> Lifespan.org Thu May 4 07:20:44 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu May 4 07:20:52 2006 Subject: [Histonet] paraffin scraper Message-ID: <09C945920A6B654199F7A58A1D7D1FDE017176ED@lsexch.lsmaster.lifespan.org> Such scapers are available from a number of different companies online. Here's one example: http://www.instaoffice.com/Long-Handle-Scraper-w-48-Handle.UNGLH12C.0.7.htm From jqb7 <@t> cdc.gov Thu May 4 07:22:59 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/NCID/VR)) Date: Thu May 4 07:23:30 2006 Subject: [Histonet] paraffin scraper Message-ID: We recently bought one from Market Lab. www.marketlabinc.com Paraffin Floor Scraper Remove paraffin to prevent slips and falls Lightweight design and 48" handle prevents back strain Printer-Friendly Version DESCRIPTION $ QTY ML1138 Paraffin Wax Scraper - 49.5" L $29.00/ea ML1139 Replacement Blades for ML1138 - 4"W $6.00/ea Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DiCarlo, Margaret Sent: Thursday, May 04, 2006 8:14 AM To: histonet@pathology.swmed.edu Subject: [Histonet] paraffin scraper Hello everyone, I am looking to purchase a very long handled paraffin scraper that you attach a razor blade at the end. This is so I can stand and scrape instead of squatting and using a putty knife. I checked the Fisher and VWR catalogs and was unsucessful. I have called at least two Home Depot stores and they don't sell them either. Can you direct me to some company or place where I can buy this ? I will appreciate your assistance. Peggy DiCarlo HT (ASCP) Orthopedics Bone Lab Buffalo General Hospital 100 High St. Buffalo, NY 14203 716-859-1293 _____ Upgrade Your Email - Click here! CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From king.laurie <@t> marshfieldclinic.org Thu May 4 07:28:56 2006 From: king.laurie <@t> marshfieldclinic.org (king.laurie@marshfieldclinic.org) Date: Thu May 4 07:28:50 2006 Subject: [Histonet] paraffin scraper Message-ID: <1256701c66f76$502f0280$6905010a@mfldclinframe.org> Try MarketLab 1-800-237-3604 or www.marketlabinc.com item#KL1138 replacement blades item#KL1139 I just ordered one, haven't received as of yet. Laurie King HT ------Original Message------ From: "DiCarlo, Margaret" Date: Thu May 04, 2006 -- 07:15:46 AM To: Subject: [Histonet] paraffin scraper Hello everyone, I am looking to purchase a very long handled paraffin scraper that you attach a razor blade at the end. This is so I can stand and scrape instead of squatting and using a putty knife. I checked the Fisher and VWR catalogs and was unsucessful. I have called at least two Home Depot stores and they don't sell them either. Can you direct me to some company or place where I can buy this ? I will appreciate your assistance. Peggy DiCarlo HT (ASCP) Orthopedics Bone Lab Buffalo General Hospital 100 High St. Buffalo, NY 14203 716-859-1293 _____ Upgrade Your Email - Click here! CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet >From histonet-bounces@lists.utsouthwestern.edu Thu May 4 07:14:58 2006 From JWEEMS <@t> sjha.org Thu May 4 08:20:17 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Thu May 4 08:22:50 2006 Subject: [Histonet] paraffin scraper References: <9B4A77DF11463E4FB723D484214AE9BC286C1D@KALEXMB02.KaleidaHealth.org> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD320263E029@sjhaexc02.sjha.org> There are ice breakers that will serve this purpose - but silver "duck" tape will hold the putty knife on the end of a broom handle (wrapped around several time) - then you can turn it around and sweep up the mess! Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of DiCarlo, Margaret Sent: Thu 5/4/2006 8:13 AM To: histonet@pathology.swmed.edu Subject: [Histonet] paraffin scraper Hello everyone, I am looking to purchase a very long handled paraffin scraper that you attach a razor blade at the end. This is so I can stand and scrape instead of squatting and using a putty knife. I checked the Fisher and VWR catalogs and was unsucessful. I have called at least two Home Depot stores and they don't sell them either. Can you direct me to some company or place where I can buy this ? I will appreciate your assistance. Peggy DiCarlo HT (ASCP) Orthopedics Bone Lab Buffalo General Hospital 100 High St. Buffalo, NY 14203 716-859-1293 _____ Upgrade Your Email - Click here! CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From Malcolm.McCallum <@t> tamut.edu Thu May 4 08:29:12 2006 From: Malcolm.McCallum <@t> tamut.edu (Malcolm McCallum) Date: Thu May 4 08:30:20 2006 Subject: [Histonet] paraffin scraper Message-ID: I don't know about one that is sold for histology, but most aquarium stores sell these. I can imagine that they would work just as well for scrapng paraffin as they do for scraping algae off of the glass. Maybe better! VISIT THE JOURNAL HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-233-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of DiCarlo, Margaret Sent: Thu 5/4/2006 7:13 AM To: histonet@pathology.swmed.edu Subject: [Histonet] paraffin scraper Hello everyone, I am looking to purchase a very long handled paraffin scraper that you attach a razor blade at the end. This is so I can stand and scrape instead of squatting and using a putty knife. I checked the Fisher and VWR catalogs and was unsucessful. I have called at least two Home Depot stores and they don't sell them either. Can you direct me to some company or place where I can buy this ? I will appreciate your assistance. Peggy DiCarlo HT (ASCP) Orthopedics Bone Lab Buffalo General Hospital 100 High St. Buffalo, NY 14203 716-859-1293 _____ Upgrade Your Email - Click here! CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo.rogerson <@t> waht.swest.nhs.uk Thu May 4 09:04:17 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu May 4 09:03:09 2006 Subject: [Histonet] paraffin scraper Message-ID: In the UK we have garden implements called hoes; I realise that does not translate well to American and the connotation but there you are we have them. They have a blade at the end of a handle and you use it to break up the soil around plants to keep weeds down and aerate the soil; you go and do some hoeing. You could use them as we have in some Labs to scrape off wax. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 The greater our awareness of intentions, the greater our freedom to choose. -- Gil Fronsdal This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Thursday, May 04, 2006 2:20 PM To: DiCarlo, Margaret; histonet@pathology.swmed.edu Subject: RE: [Histonet] paraffin scraper There are ice breakers that will serve this purpose - but silver "duck" tape will hold the putty knife on the end of a broom handle (wrapped around several time) - then you can turn it around and sweep up the mess! Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of DiCarlo, Margaret Sent: Thu 5/4/2006 8:13 AM To: histonet@pathology.swmed.edu Subject: [Histonet] paraffin scraper Hello everyone, I am looking to purchase a very long handled paraffin scraper that you attach a razor blade at the end. This is so I can stand and scrape instead of squatting and using a putty knife. I checked the Fisher and VWR catalogs and was unsucessful. I have called at least two Home Depot stores and they don't sell them either. Can you direct me to some company or place where I can buy this ? I will appreciate your assistance. Peggy DiCarlo HT (ASCP) Orthopedics Bone Lab Buffalo General Hospital 100 High St. Buffalo, NY 14203 716-859-1293 _____ Upgrade Your Email - Click here! CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From cwscouten <@t> myneurolab.com Thu May 4 09:03:02 2006 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Thu May 4 09:03:29 2006 Subject: [Histonet] Protocols for estimating the swelling of tissue? Message-ID: <5784D843593D874C93E9BADCB87342AB01306EB9@tpiserver03.Coretech-holdings.com> If you are taking a whole small organ, you can measure its volume with a Plethysmometer. But I think volume of tissue is not what you want, but a percentage of size change from normal. Not easy to get. Fixatives causes cells to swell on first exposure, but then shrink and the whole organ or tissue shrinks. There is an article coming out in the May issue of Microscopy Today that addresses these issues, but does not solve your problem. Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: Thursday, May 04, 2006 2:04 AM To: 'eboyden3@gmail.com'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protocols for estimating the swelling of tissue? I thought, and I stand to be corrected, that most fixatives caused shrinkage of tissue. I assumed that to be due to the fixation, condensation, cross linking of proteins or the eradication of water molecules. Hypertonic sucrose would also cause shrinkage too as it would exert a negative osmotic pressure on the 'more dilute' tissue, wouldn't it or have I got my pressures all wrong. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 One's action ought to come out of an achieved stillness: not to be a mere rushing on. -- D.H. Lawrence This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Ed Boyden [mailto:eboyden3@gmail.com] Sent: Thursday, May 04, 2006 12:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Protocols for estimating the swelling of tissue? Many procedures (fixation, immersion in sucrose, etc.) cause swelling of tissue, versus the intact, original tissue. Does anyone know of either: 1. ways to predict the amount of swelling that occurs with a specific protocol, or 2. a protcol that would allow swelling of the tissue by a predictable amount? I would like to be able to compare samples done at different times, and stored in different ways. If not possible, I would like to be able to prepare new samples so that their swelling will match my old estimates, as much as possible, or at least that I can calculate the swelling of the new samples. Thanks so much for your help! Best, Ed _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo.rogerson <@t> waht.swest.nhs.uk Thu May 4 09:29:47 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu May 4 09:28:40 2006 Subject: [Histonet] Protocols for estimating the swelling of tissue? Message-ID: That's interesting. Is that in all fixatives? I could understand an aqueous fixative initially causing such swelling as the water may reach the proteins before the fixative; I also understand that acids are put into fixatives in part to counteract the shrinking effects, but I would be surprised if an alcoholic fixative caused any swelling initially at all. How could it? As it removed water and caused the condensation of the protein molecules how could that make the tissue swell? If the tissue shrank or swelled then you think you could use water displacement to measure the changes but I suppose that would only measures the contraction due to increased cross linking of proteins as any water removed would enter the vessel and therefore counteract any displacement as the organ shrunk. I don't think I've understood what I've just said. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 The greater our awareness of intentions, the greater our freedom to choose. -- Gil Fronsdal This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Charles Scouten [mailto:cwscouten@myneurolab.com] Sent: Thursday, May 04, 2006 3:03 PM To: Kemlo Rogerson; eboyden3@gmail.com; histonet@lists.utsouthwestern.edu Cc: Microscopy Today Subject: RE: [Histonet] Protocols for estimating the swelling of tissue? If you are taking a whole small organ, you can measure its volume with a Plethysmometer. But I think volume of tissue is not what you want, but a percentage of size change from normal. Not easy to get. Fixatives causes cells to swell on first exposure, but then shrink and the whole organ or tissue shrinks. There is an article coming out in the May issue of Microscopy Today that addresses these issues, but does not solve your problem. Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: Thursday, May 04, 2006 2:04 AM To: 'eboyden3@gmail.com'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Protocols for estimating the swelling of tissue? I thought, and I stand to be corrected, that most fixatives caused shrinkage of tissue. I assumed that to be due to the fixation, condensation, cross linking of proteins or the eradication of water molecules. Hypertonic sucrose would also cause shrinkage too as it would exert a negative osmotic pressure on the 'more dilute' tissue, wouldn't it or have I got my pressures all wrong. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 One's action ought to come out of an achieved stillness: not to be a mere rushing on. -- D.H. Lawrence This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Ed Boyden [mailto:eboyden3@gmail.com] Sent: Thursday, May 04, 2006 12:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Protocols for estimating the swelling of tissue? Many procedures (fixation, immersion in sucrose, etc.) cause swelling of tissue, versus the intact, original tissue. Does anyone know of either: 1. ways to predict the amount of swelling that occurs with a specific protocol, or 2. a protcol that would allow swelling of the tissue by a predictable amount? I would like to be able to compare samples done at different times, and stored in different ways. If not possible, I would like to be able to prepare new samples so that their swelling will match my old estimates, as much as possible, or at least that I can calculate the swelling of the new samples. Thanks so much for your help! Best, Ed _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Thu May 4 09:36:35 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Thu May 4 09:36:44 2006 Subject: [Histonet] paraffin scraper Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202637D8E@sjhaexc02.sjha.org> The ice scraper I mentioned is like a hoe straightened out so that you can push it to scrape. We had them in Alaska. We have hoes here - especially in Tennessee where I used one for years! I am an old farm girl from way back! j Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 404-851-7831 - fax -----Original Message----- From: Kemlo Rogerson [mailto:kemlo.rogerson@waht.swest.nhs.uk] Sent: Thursday, May 04, 2006 10:04 AM To: Weems, Joyce; DiCarlo, Margaret; histonet@pathology.swmed.edu Subject: RE: [Histonet] paraffin scraper In the UK we have garden implements called hoes; I realise that does not translate well to American and the connotation but there you are we have them. They have a blade at the end of a handle and you use it to break up the soil around plants to keep weeds down and aerate the soil; you go and do some hoeing. You could use them as we have in some Labs to scrape off wax. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 The greater our awareness of intentions, the greater our freedom to choose. -- Gil Fronsdal This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Thursday, May 04, 2006 2:20 PM To: DiCarlo, Margaret; histonet@pathology.swmed.edu Subject: RE: [Histonet] paraffin scraper There are ice breakers that will serve this purpose - but silver "duck" tape will hold the putty knife on the end of a broom handle (wrapped around several time) - then you can turn it around and sweep up the mess! Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of DiCarlo, Margaret Sent: Thu 5/4/2006 8:13 AM To: histonet@pathology.swmed.edu Subject: [Histonet] paraffin scraper Hello everyone, I am looking to purchase a very long handled paraffin scraper that you attach a razor blade at the end. This is so I can stand and scrape instead of squatting and using a putty knife. I checked the Fisher and VWR catalogs and was unsucessful. I have called at least two Home Depot stores and they don't sell them either. Can you direct me to some company or place where I can buy this ? I will appreciate your assistance. Peggy DiCarlo HT (ASCP) Orthopedics Bone Lab Buffalo General Hospital 100 High St. Buffalo, NY 14203 716-859-1293 _____ Upgrade Your Email - Click here! CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From japoteete <@t> saintfrancis.com Thu May 4 10:39:22 2006 From: japoteete <@t> saintfrancis.com (Poteete, Jacquie A.) Date: Thu May 4 10:39:31 2006 Subject: [Histonet] paraffin scraper Message-ID: We ordered ours from MarketLab. The catalog number is JL1138, price $29 and the catalog number for the replacement blades is JL1139, price $6. The handle measures 48 inches, and the blade width is 4 inches. The telephone number is 1-800-237-3604, and the eir website is http://www.marketlabinc.com. You might need to hide it because they seem to be very popular with our Housekeeping Department, and the scrapers tend to wander away with the housekeepers. Jacquie Poteete MT(ASCP)QIHC Lead Technologist, IHC Laboratory Saint Francis Hospital, Tulsa, OK japoteete@saintfrancis.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Thursday, May 04, 2006 9:37 AM To: Kemlo Rogerson; DiCarlo, Margaret; histonet@pathology.swmed.edu Subject: RE: [Histonet] paraffin scraper The ice scraper I mentioned is like a hoe straightened out so that you can push it to scrape. We had them in Alaska. We have hoes here - especially in Tennessee where I used one for years! I am an old farm girl from way back! j Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 404-851-7831 - fax -----Original Message----- From: Kemlo Rogerson [mailto:kemlo.rogerson@waht.swest.nhs.uk] Sent: Thursday, May 04, 2006 10:04 AM To: Weems, Joyce; DiCarlo, Margaret; histonet@pathology.swmed.edu Subject: RE: [Histonet] paraffin scraper In the UK we have garden implements called hoes; I realise that does not translate well to American and the connotation but there you are we have them. They have a blade at the end of a handle and you use it to break up the soil around plants to keep weeds down and aerate the soil; you go and do some hoeing. You could use them as we have in some Labs to scrape off wax. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 The greater our awareness of intentions, the greater our freedom to choose. -- Gil Fronsdal This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Thursday, May 04, 2006 2:20 PM To: DiCarlo, Margaret; histonet@pathology.swmed.edu Subject: RE: [Histonet] paraffin scraper There are ice breakers that will serve this purpose - but silver "duck" tape will hold the putty knife on the end of a broom handle (wrapped around several time) - then you can turn it around and sweep up the mess! Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of DiCarlo, Margaret Sent: Thu 5/4/2006 8:13 AM To: histonet@pathology.swmed.edu Subject: [Histonet] paraffin scraper Hello everyone, I am looking to purchase a very long handled paraffin scraper that you attach a razor blade at the end. This is so I can stand and scrape instead of squatting and using a putty knife. I checked the Fisher and VWR catalogs and was unsucessful. I have called at least two Home Depot stores and they don't sell them either. Can you direct me to some company or place where I can buy this ? I will appreciate your assistance. Peggy DiCarlo HT (ASCP) Orthopedics Bone Lab Buffalo General Hospital 100 High St. Buffalo, NY 14203 716-859-1293 _____ Upgrade Your Email - Click here! CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From Terry.Marshall <@t> rothgen.nhs.uk Thu May 4 10:48:07 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Thu May 4 11:03:23 2006 Subject: [Histonet] paraffin scraper Message-ID: Of course they have hoes in America! Hoedown A hoedown is a square dance, or a gathering where a square dance is featured. The word is commonly used to describe any country or hillbilly type gathering, generally one with fiddle and banjo music. Hoedowns were originally harvest festivals of sorts, a gathering held in the fall as the crops were brought in. The farmers who attended would put their hoes down and come to a dance, hence the word "hoedown". Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:kemlo.rogerson@waht.swest.nhs.uk] Sent: 04 May 2006 15:04 To: 'Weems, Joyce'; DiCarlo, Margaret; histonet@pathology.swmed.edu Subject: RE: [Histonet] paraffin scraper In the UK we have garden implements called hoes; I realise that does not translate well to American and the connotation but there you are we have them. They have a blade at the end of a handle and you use it to break up the soil around plants to keep weeds down and aerate the soil; you go and do some hoeing. You could use them as we have in some Labs to scrape off wax. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 The greater our awareness of intentions, the greater our freedom to choose. -- Gil Fronsdal This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Thursday, May 04, 2006 2:20 PM To: DiCarlo, Margaret; histonet@pathology.swmed.edu Subject: RE: [Histonet] paraffin scraper There are ice breakers that will serve this purpose - but silver "duck" tape will hold the putty knife on the end of a broom handle (wrapped around several time) - then you can turn it around and sweep up the mess! Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of DiCarlo, Margaret Sent: Thu 5/4/2006 8:13 AM To: histonet@pathology.swmed.edu Subject: [Histonet] paraffin scraper Hello everyone, I am looking to purchase a very long handled paraffin scraper that you attach a razor blade at the end. This is so I can stand and scrape instead of squatting and using a putty knife. I checked the Fisher and VWR catalogs and was unsucessful. I have called at least two Home Depot stores and they don't sell them either. Can you direct me to some company or place where I can buy this ? I will appreciate your assistance. Peggy DiCarlo HT (ASCP) Orthopedics Bone Lab Buffalo General Hospital 100 High St. Buffalo, NY 14203 716-859-1293 _____ Upgrade Your Email - Click here! CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ploykasek <@t> phenopath.com Thu May 4 11:11:53 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Thu May 4 11:12:18 2006 Subject: [Histonet] Cam5.2 Message-ID: Hi all. I've heard a rumor that cytokeratin 8 clone CAM5.2 from Becton Dickinson is being discontinued. Has anyone else heard this or have any other information on this clone? Thanks. Also, thanks for all the good info on the reagent vials for the Autostainer. I am off to enjoy a bit of rare sunshine in our Emerald City! Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From japoteete <@t> saintfrancis.com Thu May 4 11:17:05 2006 From: japoteete <@t> saintfrancis.com (Poteete, Jacquie A.) Date: Thu May 4 11:17:10 2006 Subject: [Histonet] Cam5.2 Message-ID: Patti, I'm still using it and have heard nothing about it, but it's order time, so I'll let you know if it is still available. I'm keeping my fingers crossed. Jacquie Poteete MT(ASCP)QIHC Lead Technologist, IHC Laboratory Saint Francis Hospital, Tulsa, OK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Thursday, May 04, 2006 11:12 AM To: histonet Cc: phyllis davie Subject: [Histonet] Cam5.2 Hi all. I've heard a rumor that cytokeratin 8 clone CAM5.2 from Becton Dickinson is being discontinued. Has anyone else heard this or have any other information on this clone? Thanks. Also, thanks for all the good info on the reagent vials for the Autostainer. I am off to enjoy a bit of rare sunshine in our Emerald City! Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------ - This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charlene.Henry <@t> STJUDE.ORG Thu May 4 11:20:41 2006 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Thu May 4 11:20:58 2006 Subject: [Histonet] Cam5.2. . Message-ID: <5CB39BCA5724F349BCB748675C6CA1A2099A1C45@SJMEMXMB02.stjude.sjcrh.local> Please reply to the list because I also use this antibody and would like to know if it is being discontinued. Charlene Henry HT (ASCP), QIHC Histology/Immunohistochemistry Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Poteete, Jacquie A. Sent: Thursday, May 04, 2006 11:17 AM To: Patti Loykasek; histonet Cc: phyllis davie Subject: RE: [Histonet] Cam5.2. . Patti, I'm still using it and have heard nothing about it, but it's order time, so I'll let you know if it is still available. I'm keeping my fingers crossed. Jacquie Poteete MT(ASCP)QIHC Lead Technologist, IHC Laboratory Saint Francis Hospital, Tulsa, OK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Thursday, May 04, 2006 11:12 AM To: histonet Cc: phyllis davie Subject: [Histonet] Cam5.2 Hi all. I've heard a rumor that cytokeratin 8 clone CAM5.2 from Becton Dickinson is being discontinued. Has anyone else heard this or have any other information on this clone? Thanks. Also, thanks for all the good info on the reagent vials for the Autostainer. I am off to enjoy a bit of rare sunshine in our Emerald City! Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------ - This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJasper <@t> smdc.org Thu May 4 11:23:41 2006 From: TJasper <@t> smdc.org (Jasper, Thomas G.) Date: Thu May 4 11:23:52 2006 Subject: [Histonet] paraffin scraper Message-ID: <1A9F2A6C5762524799A816F1F09744CF1E0A80@SCREECH.ntcampus.smdc.org> Kemlo and Terry...I love it, you guys could have a sitcom or entertain at "Happy Hour". Now on a more serious note...we use a Thermo (Shandon) Electron, Para-Trimmer. Sure beats an ice scaper, dull scalpel or modified hoe. Tom J. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Marshall Terry Dr, Consultant Histopathologist Sent: Thursday, May 04, 2006 10:48 AM To: Kemlo Rogerson; Weems, Joyce; DiCarlo, Margaret; histonet@pathology.swmed.edu Subject: RE: [Histonet] paraffin scraper Of course they have hoes in America! Hoedown A hoedown is a square dance, or a gathering where a square dance is featured. The word is commonly used to describe any country or hillbilly type gathering, generally one with fiddle and banjo music. Hoedowns were originally harvest festivals of sorts, a gathering held in the fall as the crops were brought in. The farmers who attended would put their hoes down and come to a dance, hence the word "hoedown". Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:kemlo.rogerson@waht.swest.nhs.uk] Sent: 04 May 2006 15:04 To: 'Weems, Joyce'; DiCarlo, Margaret; histonet@pathology.swmed.edu Subject: RE: [Histonet] paraffin scraper In the UK we have garden implements called hoes; I realise that does not translate well to American and the connotation but there you are we have them. They have a blade at the end of a handle and you use it to break up the soil around plants to keep weeds down and aerate the soil; you go and do some hoeing. You could use them as we have in some Labs to scrape off wax. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 The greater our awareness of intentions, the greater our freedom to choose. -- Gil Fronsdal This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Thursday, May 04, 2006 2:20 PM To: DiCarlo, Margaret; histonet@pathology.swmed.edu Subject: RE: [Histonet] paraffin scraper There are ice breakers that will serve this purpose - but silver "duck" tape will hold the putty knife on the end of a broom handle (wrapped around several time) - then you can turn it around and sweep up the mess! Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of DiCarlo, Margaret Sent: Thu 5/4/2006 8:13 AM To: histonet@pathology.swmed.edu Subject: [Histonet] paraffin scraper Hello everyone, I am looking to purchase a very long handled paraffin scraper that you attach a razor blade at the end. This is so I can stand and scrape instead of squatting and using a putty knife. I checked the Fisher and VWR catalogs and was unsucessful. I have called at least two Home Depot stores and they don't sell them either. Can you direct me to some company or place where I can buy this ? I will appreciate your assistance. Peggy DiCarlo HT (ASCP) Orthopedics Bone Lab Buffalo General Hospital 100 High St. Buffalo, NY 14203 716-859-1293 _____ Upgrade Your Email - Click here! CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From MDiCarlo <@t> KaleidaHealth.Org Thu May 4 11:27:19 2006 From: MDiCarlo <@t> KaleidaHealth.Org (DiCarlo, Margaret) Date: Thu May 4 11:27:32 2006 Subject: [Histonet] paraffin scraper Message-ID: <9B4A77DF11463E4FB723D484214AE9BC286C1E@KALEXMB02.KaleidaHealth.org> Thank you everyone for info on the paraffin scraper. I am waiting for a lawson number so I can order it from Market Lab. Peggy DiCarlo HT (ASCP) Orthopedics Bone Lab Buffalo General Hospital 100 High St. Buffalo, NY 14203 716-859-1293 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of king.laurie@marshfieldclinic.org Sent: Thursday, May 04, 2006 08:29 To: histonet@pathology.swmed.edu Subject: Re: [Histonet] paraffin scraper Try MarketLab 1-800-237-3604 or www.marketlabinc.com item#KL1138 replacement blades item#KL1139 I just ordered one, haven't received as of yet. Laurie King HT ------Original Message------ From: "DiCarlo, Margaret" Date: Thu May 04, 2006 -- 07:15:46 AM To: Subject: [Histonet] paraffin scraper Hello everyone, I am looking to purchase a very long handled paraffin scraper that you attach a razor blade at the end. This is so I can stand and scrape instead of squatting and using a putty knife. I checked the Fisher and VWR catalogs and was unsucessful. I have called at least two Home Depot stores and they don't sell them either. Can you direct me to some company or place where I can buy this ? I will appreciate your assistance. Peggy DiCarlo HT (ASCP) Orthopedics Bone Lab Buffalo General Hospital 100 High St. Buffalo, NY 14203 716-859-1293 _____ Upgrade Your Email - Click here! CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet >From histonet-bounces@lists.utsouthwestern.edu Thu May 4 07:14:58 2006 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From cpomajzl <@t> cpllabs.com Thu May 4 11:35:08 2006 From: cpomajzl <@t> cpllabs.com (Chris Pomajzl) Date: Thu May 4 11:32:03 2006 Subject: [Histonet] paraffin scraper References: Message-ID: <006b01c66f98$b5573020$26fca8c0@CSP> On behalf of all Hillbillies and Texans, I am offended! ; ) ----- Original Message ----- From: "Marshall Terry Dr,Consultant Histopathologist" To: "Kemlo Rogerson" ; "Weems, Joyce" ; "DiCarlo, Margaret" ; Sent: Thursday, May 04, 2006 10:48 AM Subject: RE: [Histonet] paraffin scraper Of course they have hoes in America! Hoedown A hoedown is a square dance, or a gathering where a square dance is featured. The word is commonly used to describe any country or hillbilly type gathering, generally one with fiddle and banjo music. Hoedowns were originally harvest festivals of sorts, a gathering held in the fall as the crops were brought in. The farmers who attended would put their hoes down and come to a dance, hence the word "hoedown". Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:kemlo.rogerson@waht.swest.nhs.uk] Sent: 04 May 2006 15:04 To: 'Weems, Joyce'; DiCarlo, Margaret; histonet@pathology.swmed.edu Subject: RE: [Histonet] paraffin scraper In the UK we have garden implements called hoes; I realise that does not translate well to American and the connotation but there you are we have them. They have a blade at the end of a handle and you use it to break up the soil around plants to keep weeds down and aerate the soil; you go and do some hoeing. You could use them as we have in some Labs to scrape off wax. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 The greater our awareness of intentions, the greater our freedom to choose. -- Gil Fronsdal This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Thursday, May 04, 2006 2:20 PM To: DiCarlo, Margaret; histonet@pathology.swmed.edu Subject: RE: [Histonet] paraffin scraper There are ice breakers that will serve this purpose - but silver "duck" tape will hold the putty knife on the end of a broom handle (wrapped around several time) - then you can turn it around and sweep up the mess! Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of DiCarlo, Margaret Sent: Thu 5/4/2006 8:13 AM To: histonet@pathology.swmed.edu Subject: [Histonet] paraffin scraper Hello everyone, I am looking to purchase a very long handled paraffin scraper that you attach a razor blade at the end. This is so I can stand and scrape instead of squatting and using a putty knife. I checked the Fisher and VWR catalogs and was unsucessful. I have called at least two Home Depot stores and they don't sell them either. Can you direct me to some company or place where I can buy this ? I will appreciate your assistance. Peggy DiCarlo HT (ASCP) Orthopedics Bone Lab Buffalo General Hospital 100 High St. Buffalo, NY 14203 716-859-1293 _____ Upgrade Your Email - Click here! CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Thu May 4 11:34:36 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Thu May 4 11:34:45 2006 Subject: [Histonet] paraffin scraper Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202637DA3@sjhaexc02.sjha.org> Not me I love hoedowns! But I are one.... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Chris Pomajzl Sent: Thursday, May 04, 2006 12:35 PM To: HISTONET Subject: Re: [Histonet] paraffin scraper On behalf of all Hillbillies and Texans, I am offended! ; ) ----- Original Message ----- From: "Marshall Terry Dr,Consultant Histopathologist" To: "Kemlo Rogerson" ; "Weems, Joyce" ; "DiCarlo, Margaret" ; Sent: Thursday, May 04, 2006 10:48 AM Subject: RE: [Histonet] paraffin scraper Of course they have hoes in America! Hoedown A hoedown is a square dance, or a gathering where a square dance is featured. The word is commonly used to describe any country or hillbilly type gathering, generally one with fiddle and banjo music. Hoedowns were originally harvest festivals of sorts, a gathering held in the fall as the crops were brought in. The farmers who attended would put their hoes down and come to a dance, hence the word "hoedown". Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:kemlo.rogerson@waht.swest.nhs.uk] Sent: 04 May 2006 15:04 To: 'Weems, Joyce'; DiCarlo, Margaret; histonet@pathology.swmed.edu Subject: RE: [Histonet] paraffin scraper In the UK we have garden implements called hoes; I realise that does not translate well to American and the connotation but there you are we have them. They have a blade at the end of a handle and you use it to break up the soil around plants to keep weeds down and aerate the soil; you go and do some hoeing. You could use them as we have in some Labs to scrape off wax. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 The greater our awareness of intentions, the greater our freedom to choose. -- Gil Fronsdal This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Thursday, May 04, 2006 2:20 PM To: DiCarlo, Margaret; histonet@pathology.swmed.edu Subject: RE: [Histonet] paraffin scraper There are ice breakers that will serve this purpose - but silver "duck" tape will hold the putty knife on the end of a broom handle (wrapped around several time) - then you can turn it around and sweep up the mess! Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of DiCarlo, Margaret Sent: Thu 5/4/2006 8:13 AM To: histonet@pathology.swmed.edu Subject: [Histonet] paraffin scraper Hello everyone, I am looking to purchase a very long handled paraffin scraper that you attach a razor blade at the end. This is so I can stand and scrape instead of squatting and using a putty knife. I checked the Fisher and VWR catalogs and was unsucessful. I have called at least two Home Depot stores and they don't sell them either. Can you direct me to some company or place where I can buy this ? I will appreciate your assistance. Peggy DiCarlo HT (ASCP) Orthopedics Bone Lab Buffalo General Hospital 100 High St. Buffalo, NY 14203 716-859-1293 _____ Upgrade Your Email - Click here! CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From Terry.Marshall <@t> rothgen.nhs.uk Thu May 4 11:35:23 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Thu May 4 11:34:55 2006 Subject: [Histonet] paraffin scraper Message-ID: BTW - after the silliness - I'm sure a hoe is the best implement if it's getting it off the floor to which you aspire. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: DiCarlo, Margaret [mailto:MDiCarlo@KaleidaHealth.Org] Sent: 04 May 2006 17:27 To: king.laurie@marshfieldclinic.org; histonet@pathology.swmed.edu Subject: RE: [Histonet] paraffin scraper Thank you everyone for info on the paraffin scraper. I am waiting for a lawson number so I can order it from Market Lab. Peggy DiCarlo HT (ASCP) Orthopedics Bone Lab Buffalo General Hospital 100 High St. Buffalo, NY 14203 716-859-1293 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of king.laurie@marshfieldclinic.org Sent: Thursday, May 04, 2006 08:29 To: histonet@pathology.swmed.edu Subject: Re: [Histonet] paraffin scraper Try MarketLab 1-800-237-3604 or www.marketlabinc.com item#KL1138 replacement blades item#KL1139 I just ordered one, haven't received as of yet. Laurie King HT ------Original Message------ From: "DiCarlo, Margaret" Date: Thu May 04, 2006 -- 07:15:46 AM To: Subject: [Histonet] paraffin scraper Hello everyone, I am looking to purchase a very long handled paraffin scraper that you attach a razor blade at the end. This is so I can stand and scrape instead of squatting and using a putty knife. I checked the Fisher and VWR catalogs and was unsucessful. I have called at least two Home Depot stores and they don't sell them either. Can you direct me to some company or place where I can buy this ? I will appreciate your assistance. Peggy DiCarlo HT (ASCP) Orthopedics Bone Lab Buffalo General Hospital 100 High St. Buffalo, NY 14203 716-859-1293 _____ Upgrade Your Email - Click here! CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet >From histonet-bounces@lists.utsouthwestern.edu Thu May 4 07:14:58 2006 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From japoteete <@t> saintfrancis.com Thu May 4 11:37:47 2006 From: japoteete <@t> saintfrancis.com (Poteete, Jacquie A.) Date: Thu May 4 11:37:53 2006 Subject: [Histonet] Cam5.2. . Message-ID: We were just told by BD that they have 650 vials of CAM 5.2 in stock. That was about 5 minutes ago. Of course they didn't have any information about it being discontinued in the near future. Jacquie Poteete MT(ASCP)QIHC Lead Technologist, IHC Laboratory Saint Francis Hospital, Tulsa, OK japoteete@saintfrancis.com -----Original Message----- From: Henry, Charlene [mailto:Charlene.Henry@STJUDE.ORG] Sent: Thursday, May 04, 2006 11:21 AM To: Poteete, Jacquie A.; Patti Loykasek; histonet Cc: phyllis davie Subject: RE: [Histonet] Cam5.2. . Please reply to the list because I also use this antibody and would like to know if it is being discontinued. Charlene Henry HT (ASCP), QIHC Histology/Immunohistochemistry Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Poteete, Jacquie A. Sent: Thursday, May 04, 2006 11:17 AM To: Patti Loykasek; histonet Cc: phyllis davie Subject: RE: [Histonet] Cam5.2. . Patti, I'm still using it and have heard nothing about it, but it's order time, so I'll let you know if it is still available. I'm keeping my fingers crossed. Jacquie Poteete MT(ASCP)QIHC Lead Technologist, IHC Laboratory Saint Francis Hospital, Tulsa, OK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Thursday, May 04, 2006 11:12 AM To: histonet Cc: phyllis davie Subject: [Histonet] Cam5.2 Hi all. I've heard a rumor that cytokeratin 8 clone CAM5.2 from Becton Dickinson is being discontinued. Has anyone else heard this or have any other information on this clone? Thanks. Also, thanks for all the good info on the reagent vials for the Autostainer. I am off to enjoy a bit of rare sunshine in our Emerald City! Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------ - This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynne.Bell <@t> hitchcock.org Thu May 4 11:58:21 2006 From: Lynne.Bell <@t> hitchcock.org (Bell, Lynne) Date: Thu May 4 12:00:06 2006 Subject: [Histonet] CAP inspections Message-ID: Our first unannounced inspection is due anytime between July and October. I'll be sure to let everyone know just how much fun it wasn't! Lynne A. Bell, HT (ASCP) Laboratory Central Vermont Hospital P.O. Box 547 Barre, VT 05641 (802)371-4122 ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Garcia, Amanda Sent: Wed 5/3/2006 3:15 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP inspections Has anyone recently gone through their CAP "unannounced" inspection and if so, how did it differ from the way inspections were previously conducted. ( if there were any differences other than being unannounced) Thanks for anyone willing to share their experience. > Amanda (Amy) Garcia > Histology/Pathology > College Station Medical Center > (979) 680-5372 office > (979) 696-5446 fax > *Mailto:amanda.garcia@triadhospitals.com > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the e-mail or any > of its attachments, please be advised that you have received this > e-mail in error and that any use, dissemination, distribution, > forwarding, printing, or copying of this e-mail or any attached files > is strictly prohibited. If you have received this e-mail in error, > please immediately purge it and all attachments and notify the sender > by reply e-mail or contact the sender at the number listed. > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From toshabana <@t> hotmail.com Thu May 4 12:00:12 2006 From: toshabana <@t> hotmail.com (Shabana Shabbeer) Date: Thu May 4 12:00:22 2006 Subject: [Histonet] BrdU staining Message-ID: Does any one know if BrdU can be used to quantitate the proliferation of slow growing tumors? If yes, does any one have a protocol to share and especially if it can be incorporated in drinking water. Which company has the best available antibody to the same? Thanks, Shabana From lrichey <@t> u.washington.edu Thu May 4 12:06:14 2006 From: lrichey <@t> u.washington.edu (Lori Richey) Date: Thu May 4 12:06:33 2006 Subject: [Histonet] paraffin scraper In-Reply-To: <9B4A77DF11463E4FB723D484214AE9BC286C1D@KALEXMB02.KaleidaHealth.org> References: <9B4A77DF11463E4FB723D484214AE9BC286C1D@KALEXMB02.KaleidaHealth.org> Message-ID: <445A3486.8000601@u.washington.edu> Marketlab carries one with replaceable blades. www.marketlabinc.com DiCarlo, Margaret wrote: >Hello everyone, > >I am looking to purchase a very long handled paraffin scraper that you attach a razor blade at the end. This is so I can stand and scrape instead of squatting and using a putty knife. I checked the Fisher and VWR catalogs and was unsucessful. I have called at least two Home Depot stores and they don't sell them either. Can you direct me to some company or place where I can buy this ? > >I will appreciate your assistance. > >Peggy DiCarlo HT (ASCP) >Orthopedics Bone Lab >Buffalo General Hospital >100 High St. >Buffalo, NY 14203 >716-859-1293 > > > > _____ > > Upgrade Your Email - Click here! > > > >CONFIDENTIALITY NOTICE: >This email transmission and any documents, files, >or previous e-mail messages attached to it are >confidential and intended solely for the use of the >individual or entity to whom they are addressed. >If you are not the intended recipient, or a person >responsible for delivering it to the intended recipient, >you are hereby notified that any further review, >disclosure, copying, dissemination, distribution, or >use of any of the information contained in or attached >to this e-mail transmission is strictly prohibited. >If you have received this message in error, please >notify the sender immediately by e-mail, discard >any paper copies, and delete all electronic files >of the message. If you are unable to contact the >sender or you are not sure as to whether you >are the intended recipient, please e-mail >ISTSEC@KaleidaHealth.org or call (716) 859-7777. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From LGaliotto <@t> nch.org Thu May 4 14:14:21 2006 From: LGaliotto <@t> nch.org (Galiotto, Laura) Date: Thu May 4 14:14:32 2006 Subject: [Histonet] Technicians need to be certified Message-ID: <270614B321ACB44D8C1D91F4F921FDC3036CBCEA@NCH01EX02.nch.org> Does anyone know of ASCP or CAP requiring Histology Technicians to be certified ? I sent the director of ASCP an e-mail, I have not got a response yet. Laura Galiotto, HT (ASCP) Histology Facilitator Northwest Community Hospital 800 West Central Road Arlington Heights, Il 60005 847-618-6190 From clcses <@t> gmail.com Thu May 4 14:15:22 2006 From: clcses <@t> gmail.com (Carmen Contreras-Sesvold) Date: Thu May 4 14:22:25 2006 Subject: [Histonet] urgent!!- referance to cite immunofluorescence on cryoprotected lungs Message-ID: <46a3be380605041215l6ddbb603lcd268e4a7c67f121@mail.gmail.com> OK histonetters I need your help... Does anyone immunofluorescently label cryopreserved lung out there? I am looking for a referance or a name anything to cite. I have done a search and am looking for anyone who hang perfuses the lung cryoprotects it followed by immunoflourescence and counter stainging to reduce background. Thanks so much Carmen From Charles.Embrey <@t> carle.com Thu May 4 14:53:18 2006 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Thu May 4 14:53:38 2006 Subject: FW: [Histonet] Technicians need to be certified Message-ID: -----Original Message----- From: Charles.Embrey Sent: Thursday, May 04, 2006 2:32 PM To: 'Galiotto, Laura' Subject: RE: [Histonet] Technicians need to be certified I am not quite sure that I understand your question. Neither CAP nor ASCP require a person to be certified to cut blocks and slides, do stains or embed tissue. On the other hand I don't think that you can honestly refer to yourself as a Histology "Technician" unless you have the certification to back it up and make it official. The state of Florida does require state licensure as an HT or HTL to do these things but that is different from a national standard or licensure. Charles Embrey Jr. PA(ASCP) www.GreyRealm.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Galiotto, Laura Sent: Thursday, May 04, 2006 2:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Technicians need to be certified Does anyone know of ASCP or CAP requiring Histology Technicians to be certified ? I sent the director of ASCP an e-mail, I have not got a response yet. Laura Galiotto, HT (ASCP) Histology Facilitator Northwest Community Hospital 800 West Central Road Arlington Heights, Il 60005 847-618-6190 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gintini <@t> buffalo.edu Thu May 4 14:05:20 2006 From: gintini <@t> buffalo.edu (Beppe Intini) Date: Thu May 4 15:09:07 2006 Subject: [Histonet] antibody anti human BMP2 Message-ID: Hi Histonetters. Can anyone suggest me a good (good reactivity on western blots and paraffin histochemistry) for a primary anti-human BMP2? Thanks -- Giuseppe Intini Clinical Assistant Professor - Periodontics PhD Candidate - Oral Biology University at Buffalo Buffalo, NY 14214 From Tiffany.L.Sheffield <@t> uth.tmc.edu Thu May 4 15:21:08 2006 From: Tiffany.L.Sheffield <@t> uth.tmc.edu (Sheffield, Tiffany L) Date: Thu May 4 15:21:20 2006 Subject: [Histonet] antibody anti human BMP2 Message-ID: Try http://www.researchd.com/index.htm I have seen there Ab used for Western blot in a few journal articles. Hope this helps ************************************************** Tiffany Sheffield-Lopez,BS,HT(ASCP) Supervisor, Bone Histomorphometry & Biomaterials Lab Department of Orthopaedic Surgery The University of Texas Houston Health Science Center 6431 Fannin, Suite 6.144 MSB Houston, TX 77030 713-500-6803 Wk 713-500-0729 Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Beppe Intini Sent: Thursday, May 04, 2006 2:05 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] antibody anti human BMP2 Hi Histonetters. Can anyone suggest me a good (good reactivity on western blots and paraffin histochemistry) for a primary anti-human BMP2? Thanks -- Giuseppe Intini Clinical Assistant Professor - Periodontics PhD Candidate - Oral Biology University at Buffalo Buffalo, NY 14214 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charles.Embrey <@t> carle.com Thu May 4 15:43:04 2006 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Thu May 4 15:43:23 2006 Subject: [Histonet] Technicians need to be certified Message-ID: Please know that I am not trying to be "harsh". My post was in no way meant to belittle anyone or offend. The terms technician technologist and specialist used in the lab represent a level of certification. Individuals work hard and study either in school or on their own to challenge the test to become a Histologic Technician (HT) or Histologic Technologist (HTL). There is no federal mandate that requires either one of these certifications to do the job but the certification allows you to be addressed as such and to place the initials after your name. With your years of experience you can probably work circles around newly certified techs and I wasn't implying you couldn't. I, myself, didn't take my HT exam until I was nearing retirement from the Air Force and had been doing the job since I was eighteen. When I refinished my basement I ran all the wire and put in all the light switches and outlets but I don't think I can refer to myself as an Electrician. It is my own opinion, but I feel to actually call yourself a Histologic Technician requires some sort of validation. Charles Embrey, PA(ASCP),HT(ASCP) -----Original Message----- From: Garcia, Amanda [mailto:Amanda.Garcia@TriadHospitals.com] Sent: Thursday, May 04, 2006 3:08 PM To: Charles.Embrey Subject: RE: [Histonet] Technicians need to be certified I am going to have to disagree with you because I can "honestly" call myself a histology technician since I have been one for 20 years now without a "certificate" to back it up and have become a histology supervisor during these 20 years. All without a "certificate". Not that there is anything wrong with acquiring your certification but I think the statement on not being able to honestly refer to yourself as a technician is rather harsh. So what am I? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles.Embrey Sent: Thursday, May 04, 2006 2:53 PM To: Histonet@pathology.swmed.edu Subject: FW: [Histonet] Technicians need to be certified -----Original Message----- From: Charles.Embrey Sent: Thursday, May 04, 2006 2:32 PM To: 'Galiotto, Laura' Subject: RE: [Histonet] Technicians need to be certified I am not quite sure that I understand your question. Neither CAP nor ASCP require a person to be certified to cut blocks and slides, do stains or embed tissue. On the other hand I don't think that you can honestly refer to yourself as a Histology "Technician" unless you have the certification to back it up and make it official. The state of Florida does require state licensure as an HT or HTL to do these things but that is different from a national standard or licensure. Charles Embrey Jr. PA(ASCP) www.GreyRealm.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Galiotto, Laura Sent: Thursday, May 04, 2006 2:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Technicians need to be certified Does anyone know of ASCP or CAP requiring Histology Technicians to be certified ? I sent the director of ASCP an e-mail, I have not got a response yet. Laura Galiotto, HT (ASCP) Histology Facilitator Northwest Community Hospital 800 West Central Road Arlington Heights, Il 60005 847-618-6190 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bliven.laura <@t> marshfieldclinic.org Thu May 4 15:48:08 2006 From: bliven.laura <@t> marshfieldclinic.org (bliven.laura@marshfieldclinic.org) Date: Thu May 4 15:47:50 2006 Subject: [Histonet] Breast Marker Question Message-ID: <1547801c66fbc$0cabd380$6905010a@mfldclinframe.org> What are the top two best markers for labeling the basement membrane components and myoepithelial cells of breast tissue (or maybe not the myoepithelial cells)? We're looking for antibodies that would assist in differentiating between invasive and non-invasive breast carcinomas. One of our pathologists wants Laminin and Collagen Type IV, but the article I found was real old. Are these two antibodies possibilities or are labs using other more sensitive and/or specific markers. Thanks, Laura Laura Bliven Histology Lab/IHC Marshfield Laboratories 1000 N. Oak Ave. Marshfield, WI 54449 From ploykasek <@t> phenopath.com Thu May 4 16:16:11 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Thu May 4 16:16:51 2006 Subject: [Histonet] Breast Marker Question In-Reply-To: <1547801c66fbc$0cabd380$6905010a@mfldclinframe.org> Message-ID: Laura, hi. We do quite a bit of that type of determination here. We have found that antibodies to smooth muscle myosin heavy chain and p63 are the best markers. SMMHC is cytoplasmic & P63 is nuclear - sometimes on a case one will look better than the other. It's nice as a double stain, too. We recently tested maspin and p-cadherin, but in our testing they were inferior. Antibodies to calponin can be used, but that is much less specific. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > What are the top two best markers for labeling the basement membrane > components and myoepithelial cells of breast tissue (or maybe not the > myoepithelial cells)? We're looking for antibodies that would assist in > differentiating between invasive and non-invasive breast carcinomas. One of > our pathologists wants Laminin and Collagen Type IV, but the article I found > was real old. Are these two antibodies possibilities or are labs using other > more sensitive and/or specific markers. > Thanks, > Laura > > Laura Bliven > Histology Lab/IHC > Marshfield Laboratories > 1000 N. Oak Ave. > Marshfield, WI 54449 > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From liz <@t> premierlab.com Thu May 4 17:53:58 2006 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu May 4 17:54:23 2006 Subject: [Histonet] tubulin and pHH3 Message-ID: <001f01c66fcd$a0b0d4c0$0300a8c0@Chlipala> Is anyone out there familar with staining for tuulin and pHH3 on frozen or paraffin embedded tissues. I know that you can stain for tubulin in cell culture but I was not certain that you could stain in frozen or paraffin sections. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 From DDDeltour <@t> mar.med.navy.mil Fri May 5 06:10:03 2006 From: DDDeltour <@t> mar.med.navy.mil (Deltour, Douglas D. (HM2)) Date: Fri May 5 06:10:42 2006 Subject: [Histonet] Microwave and DNA/Molecular Message-ID: <3F500F8B416C554EBB21FF16642F72E959CE73@marxchg03.mar.med.navy.mil> Hello, I am looking for some information that can confirm or deny that microwave technology would degrade DNA and limit molecular testing. Thanks! DOUGLAS D. DELTOUR HT(ASCP) HISTOLOGY TECHNICIAN NMC PORTSMOUTH VA (757)953-1525 From ahaines <@t> vims.edu Fri May 5 07:14:53 2006 From: ahaines <@t> vims.edu (Ashley Haines) Date: Fri May 5 07:15:03 2006 Subject: [Histonet] texture/quality of DAB staining Message-ID: <000901c6703d$845e5b10$be07468b@campus.vims.edu> Hi folks, I am posting a picture called "fine DAB staining" (http://www.histonet.org/site_images_frame.asp). I am hoping for suggestions on how to improve the quality of the staining. In some senses it's a great result. You can see, at least under the scope if not in the posted micrograph, lots of fine detail. There are many fine projections from the cell surface which stain positive. Unfortunately, my goal is to counterstain with a Wright Geimsa-like or Hema3 stain. The signal I am getting will not be visible with that counterstain. Can you suggest any ways to make my staining coarser and/or darker. Here's my basic protocol for the posted micrograph: MeOH fix cytospin, block with 3% H202 in MeOH 1hr at RT, wash, block with 10% goat serum 1 hr, wash, incubate with biotinylated primary 1hr at 37, wash, incubate with SA-HRP (1:1000) for 1 hr at RT, wash, detect with Vector's DAB substrate kit (with nickel enhancement) 20 min at RT, wash in DI. Thanks! Ashley From Jackie.O'Connor <@t> abbott.com Fri May 5 07:24:54 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri May 5 07:25:21 2006 Subject: [Histonet] Pancreas markers In-Reply-To: Message-ID: Happy Friday Histonetters - I'm looking for some ideas for pancreas specific markers - not just islet cells, but antibodies to identify pancreas tumors. Any clues? Jackie O' From john_paul1959 <@t> yahoo.com Fri May 5 08:56:58 2006 From: john_paul1959 <@t> yahoo.com (John Paul) Date: Fri May 5 08:57:03 2006 Subject: [Histonet] counter-top tissue processors Message-ID: <20060505135659.46709.qmail@web31315.mail.mud.yahoo.com> Hi everyone We are looking to buy a counter-top (new or used) tissue processor (paraffin) for a small research lab. I would appreciate any recommendations from the group. Have a great day!! John --------------------------------- Yahoo! Mail goes everywhere you do. Get it on your phone. From livieira <@t> ualg.pt Fri May 5 10:26:12 2006 From: livieira <@t> ualg.pt (Lina Vieira) Date: Fri May 5 10:28:19 2006 Subject: [Histonet] Sudan Black for lipids Thank You Message-ID: <002c01c67058$3e339a40$2914100a@labhistologia> I would like to thank Gayle, Rene and Margaret for their answers to my question about Sudan Black. Thank You! Rene have asked me about where I am writing from, I` m writing from Faro, a city in Sud of Portugal! Best regards, Lina Vieira University of Algarve ITUCCA Laboratory of Histology Faro Portugal From thoward <@t> unm.edu Fri May 5 11:01:59 2006 From: thoward <@t> unm.edu (Tamara Howard) Date: Fri May 5 11:02:03 2006 Subject: [Histonet] Contract histo lab for research material? (USA) Message-ID: I'm not sure how I got involved in this, but....A research lab back east is looking for a contract lab to do some praffin embedding and sectioning; I don't know if they also need staining/immunos/ISH, what the volume of samples will be, etc. Sorry. These would be animal samples. I don't actually know the lab - someone they asked then asked me if I could find out. Oh, we don't think the geographic location of the contract lab is an issue, so any labs in the USA may be fair game. Replies to the list or off-line will be fine. Thanks for your help! Tamara |--------------------------------------------------| Tamara Howard Department of Cell Biology and Physiology University of New Mexico - Health Sciences Center Albuquerque, NM 87131 thoward@unm.edu |--------------------------------------------------| From John.MacDougall <@t> serono.com Fri May 5 11:19:32 2006 From: John.MacDougall <@t> serono.com (John.MacDougall@serono.com) Date: Fri May 5 11:20:13 2006 Subject: [Histonet] Position open Message-ID: Hi Histonetters We have a position in the Boston area (Rockland, MA) open for a research histologist with MSc, 2-5 years of experience. Mostly histology work, but experience with other lab techniques is preferred. Oncology background is also desirable. Job code is 0600212. Please visit www.serono.com/careers to submit your CV. Thanks John MacDougall, Ph.D. Senior Principal Investigator - Biotherapeutic Discovery Research and Pharmaceutical Development Serono Research Institute One Technology Place Rockland, MA 02370 U.S.A. Voice - 1-781-681-2872 Fax - 1-781-681-2910 From Julie.Sanders <@t> va.gov Fri May 5 11:21:15 2006 From: Julie.Sanders <@t> va.gov (Sanders, Julie, VHACIN) Date: Fri May 5 11:21:21 2006 Subject: [Histonet] FW: H&E on Leica XL automatic stainer Message-ID: <2D4ACE41DEFE93428F23D77988EFBCB50E5FA9@VHAV10MSGA2.v10.med.va.gov> -----Original Message----- From: Sanders, Julie, VHACIN Sent: Friday, May 05, 2006 11:28 AM To: histonet@lists.utsouthwestern.ed; 200/316 Unmatched Invoices Subject: H&E on Leica XL automatic stainer Hi Netters, I am trying to find different protocols for H&E on the Leica autostainer XL. I would appreciate anyone using this stainer sharing their procedure. Currently we are using Gills lll and Richard Allen Eosin...but I am interested in any/all procedures. Feel free to email them to me privately if you wish. Thanks in advance, Julie Julie A. Sanders, BA,HT(ASCP) Supervisor, Anatomic Pathology Cincinnati VAMC Julie.Sanders@va.gov From LINDA.MARGRAF <@t> childrens.com Fri May 5 11:55:48 2006 From: LINDA.MARGRAF <@t> childrens.com (LINDA MARGRAF) Date: Fri May 5 11:56:11 2006 Subject: [Histonet] Mohs lab supervisor opening Message-ID: Here's a job opening Marquita asked me to post. Please respond to her or Danna and not me. Thanks! Linda M Histonet administrator Type: Mohs Lab SupervisorTitle: Chief Histology TechThe Dermatologic Surgery Division of UT Southwestern in Dallas is looking for a Mohs lab supervisor with 1 year supervisory experience and 7 years histology experience. Current practice includes 2 Mohs surgeons with one or two surgeons beginning in Sept 06. Oversee all laboratory operations. Cut and prepare frozen skin sections using the Mohs technique. Immunostaining technique for Mohs sections. Maintain CLIA certification for a Mohs lab. Manage 3-4 laboratory staff. Computer literacy and software knowledge needed: Word and Excel. Excellent benefits. Respond directly to Mrs. Danna Hightower, Clinic Manager danna.hightower@utsouthwestern.edu FAX 214-648-1587Telephone 214-648-5757 Marquita Wiggins Manager Dermatology Dept UT Southwestern Medical Center 5323 Harry Hines Blvd, Room F4.100 Dallas, TX 75390-9069 214-648-2132 marquita.wiggins@utsouthwestern.edu From LuckG <@t> empirehealth.org Fri May 5 12:22:19 2006 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Fri May 5 12:24:13 2006 Subject: [Histonet] Job Opening Message-ID: Hello all, We have an histology position open for an HT or HTL certifed tech. Routine histology duties (embedding, cutting and special stains. IHC experience in addition preferred. This position is in a 300 bed community hospital in Spokane, WA. Below I have placed three weblinks for those interested so that they can go on the internet to look at our facility, corporate organization and the community and surrounding region. A great place to work and live. Please contact me directly. Thanks, Greg www.empirehealth.org www.deaconessmc.org www.visitspokane.com Greg Luck Anatomic Path Spvr Deaconess Med Cntr 800 W. 5th Ave Spokane, WA 99204 Offc 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org From TJJ <@t> Stowers-Institute.org Fri May 5 13:13:59 2006 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Fri May 5 13:14:38 2006 Subject: [Histonet] Re: Microwave and DNA/Molecular Message-ID: Doug, I think the jury is still out on that. Microwave radiation is non-ionizing electromagnetic energy similar to radar and sound waves. There have been some reports of state troopers getting tumors due to the radar guns, or folks having tumors from using cell phones. I don't know how good the science is behind it, but I'm thinking you'll find reports which state they do cause damage, and others which state they don't. If you're talking about what a microwave tissue processor would do to your DNA in the sample, I believe the radiation would not harm it. The molecules will vibrate in response to exposure to the microwaves and that is what generates the heat. Keeping the temperature controlled should keep the DNA from denaturing. High temperatures in the microwave, as those achieved doing HIER techniques, will surely cause DNA strands to separate. However, I suspect cooling should allow them to pair back up. But for routine processing, the heat never gets that high. I found information on this website (http://www.gallawa.com/microtech/mwave.html) which doesn't directly answer your question, but it does provide good information. Kok and Boon also have a book on Microwave technology you can buy through Milestone Medical. Good luck, Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64133 From cwscouten <@t> myneurolab.com Fri May 5 14:41:44 2006 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Fri May 5 14:42:13 2006 Subject: [Histonet] FW: MyNeuroLab.com Media Message-ID: <5784D843593D874C93E9BADCB87342AB01306EE3@tpiserver03.Coretech-holdings.com> It sounds like she is making a Ralph knife for sectioning GMA/JB-4/HistOresin. Yes, these knife makers are still available. Glass knives are used extensively in EM labs but made in a different configuration, a triangled knife. It sounds like the knife breaker is in bad need of service. The knife scoreing wheel probably needs to be replaced, adjusted along with a few other replaceable parts. What model of glass breaker are you using? Lee Lee E. Dickey Product Marketing and Sales Manager McCormick Scientific ________________________________ From: Tabitha Granshaw [mailto:TGranshaw@biocompare.com] Sent: Wednesday, May 03, 2006 5:05 PM To: Charles Scouten Cc: Marla Steuer Subject: RE: MyNeuroLab.com Media Hi Charles, I just wanted to drop you a line to see if MyNeuroLab would be interested in a Promotion. This Promotion would be covered by the contract so there would be no additional charge. The Promotions can be viewed on the Biocompare site at http://www.biocompare.com/contents/9/Promotions-and-Special-Offers.html If you would be interested in setting one up, don't hesitate to drop me a line Cheers, Tabitha ________________________________ From: Charles Scouten [mailto:cwscouten@myneurolab.com] Sent: Friday, March 31, 2006 9:55 AM To: Tabitha Granshaw Subject: RE: MyNeuroLab.com Media Thanks, thats fine. Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com ________________________________ From: Tabitha Granshaw [mailto:TGranshaw@biocompare.com] Sent: Friday, March 31, 2006 11:08 AM To: Charles Scouten Cc: Marla Steuer Subject: RE: MyNeuroLab.com Media Good Morning Charles, The two sponsorship placements would be covered by the current contract, so there would be no additional charge. Since it sounds like the sponsorships are okay with you, I've gone ahead and scheduled them for: April 20 May 25 As the text I sent to you yesterday looks okay, I'll go ahead and place that in the two sponsorship spots. Drop me a line if you have any questions Cheers, Tabitha Tabitha Granshaw Manager of Media Coordination Biocompare The Buyer's Guide for Life Scientists 395 Oyster Point Blvd. Suite 405 South San Francisco, CA 94080 Phone (650) 416-0505 http://www.biocompare.com ________________________________ From: Charles Scouten [mailto:cwscouten@myneurolab.com] Sent: Friday, March 31, 2006 7:31 AM To: Tabitha Granshaw Subject: RE: MyNeuroLab.com Media yes, the attached text would be fine. I am a little confused, am I hereby authorizing a funds expenditure? I have not previosuly been in on what we do with these, and know we are short of cash these day. Sponsorship sounds like if costs us money. How much? Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com ________________________________ From: Tabitha Granshaw [mailto:TGranshaw@biocompare.com] Sent: Thursday, March 30, 2006 6:33 PM To: Charles Scouten Cc: Marla Steuer Subject: MyNeuroLab.com Media Hi Charles, Thanks for sending over the text for the New Technology - I've started the postig process for that and will let you know when it's live. Also, I'd like to schedule a couple Neuroscience sponsorships for MyNeuroLab. If you like we could schedule April 20 for MyNeuroLab.com, and perhaps also a date in May. Would the attached text be okay to run? (It's the text that was used for the most recent MyNeuroLab.com sponsorship) If you could let me know about this soon, that would be great as these spots go quickly Thanks Tabitha Tabitha Granshaw Manager of Media Coordination Biocompare The Buyer's Guide for Life Scientists 395 Oyster Point Blvd. Suite 405 South San Francisco, CA 94080 Phone (650) 416-0505 http://www.biocompare.com From mari.ann.mailhiot <@t> leica-microsystems.com Fri May 5 14:49:36 2006 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Fri May 5 14:49:43 2006 Subject: [Histonet] FW: H&E on Leica XL automatic stainer In-Reply-To: <2D4ACE41DEFE93428F23D77988EFBCB50E5FA9@VHAV10MSGA2.v10.med.va.gov> Message-ID: Hi Julie I will be happy to send you some protocols for the AutoXL if you would like. Please let me know Best Regards Mari Ann Mailhiot BA HT ASCP Application Specialist Leica Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com "Sanders, Julie, VHACIN" Sent by: cc histonet-bounces@ lists.utsouthwest Subject ern.edu [Histonet] FW: H&E on Leica XL automatic stainer 05/05/2006 11:21 AM -----Original Message----- From: Sanders, Julie, VHACIN Sent: Friday, May 05, 2006 11:28 AM To: histonet@lists.utsouthwestern.ed; 200/316 Unmatched Invoices Subject: H&E on Leica XL automatic stainer Hi Netters, I am trying to find different protocols for H&E on the Leica autostainer XL. I would appreciate anyone using this stainer sharing their procedure. Currently we are using Gills lll and Richard Allen Eosin...but I am interested in any/all procedures. Feel free to email them to me privately if you wish. Thanks in advance, Julie Julie A. Sanders, BA,HT(ASCP) Supervisor, Anatomic Pathology Cincinnati VAMC Julie.Sanders@va.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From anh2006 <@t> med.cornell.edu Fri May 5 16:21:28 2006 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Fri May 5 16:21:42 2006 Subject: [Histonet] counter-top tissue processors Message-ID: <47ec5a7c1d205.445b8998@med.cornell.edu> In the past I used the Leica TP1020 and found it to be *very* unsatisfactory - in fact we returned it and got an automated Leica TP1050 which was a dream to work with. I suggest you get a full sized processor as in my experience they are far superior. If you have cost concerns get a refurbished one but buy from a reliable instrument company. ----- Original Message ----- From: John Paul Date: Friday, May 5, 2006 9:56 am Subject: [Histonet] counter-top tissue processors > Hi everyone > We are looking to buy a counter-top (new or used) tissue > processor (paraffin) for a small research lab. I would appreciate > any recommendations from the group. > Have a great day!! > John > > > --------------------------------- > Yahoo! Mail goes everywhere you do. Get it on your phone. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From peptolab <@t> hamptons.com Fri May 5 16:28:37 2006 From: peptolab <@t> hamptons.com (Jeff Silverman) Date: Fri May 5 16:29:16 2006 Subject: [Histonet] Marker for invasive carcinoma Message-ID: <000001c6708a$df34b910$6401a8c0@jeffrey028c8d9> Laura, Finally, my research interest is finding some use in practice :-). In the breast and in many other organs, the normal resting periductal, perilobular, and interstitial fibroblasts express CD34. As carcinomatous invasion occurs, resting CD34+ dendritic stromal fibroblasts transform into actin+ myofibroblasts so analysis of these two stromal markers are useful in evaluating the presence or absence of carcinomatous invasion. Double stains for cytokeratin and alpha smooth muscle actin have been used to demonstrate the presence of invasion ie carcinoma cells surrounded by or amidst myofibroblasts. These contractile cells are necessary to effect migration through the connective tissue. The actin antibody will also detect the presence or absence of myoepithelial cells in a given lesion. http://www.blackwell-synergy.com/links/doi/10.1046%2Fj.1365-2559.1996.d01-51 0.x http://jcp.bmjjournals.com/cgi/content/full/56/4/271 This CD34 versus smooth muscle actin approach was recently reported useful in evaluating invasive implants of peritoneal mesothelioma versus benign endosalpingiosis in omentum and peritoneum. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve &db=pubmed&dopt=Abstract&list_uids=16415795&query_hl=1&itool=pubmed_docsum Hope this helps. Jeff Silverman HT HTL QIHC (ASCP) Pathologists' Assistant- Pathology Supervisor Southside Hospital North Shore Long Island Jewish Health System Bay Shore, New York USA From anh2006 <@t> med.cornell.edu Fri May 5 16:34:14 2006 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Fri May 5 16:34:21 2006 Subject: [Histonet] texture/quality of DAB staining Message-ID: <48ca4e7d1ed65.445b8c96@med.cornell.edu> Hi Ashley, Your pic isn't on the site yet so I haven't been able to see precisely what you are talking about but I have a few suggestions to increase staining based on your protocol: (1) I am assuming from your protocol and desire to counterstain with WG that you are doing cytospins of fresh cells???? (2) Did you try other fixations besides methanol? I find that methanol sometimes can reduce staining for certain antigens? (3) Use 0.3% H202 in H20 for 10 minutes as 3% is harsh for cytospins or fresh tissue. (4) Add another amplification step such as using a primary, biotinylated secondary and then Strep-HRP. Using a directly labeled primary will reduce your overall amplification. (5) You could try Vector's ABC instead of Strep-HRP, your staining will be more intense though be careful for background (6) Are you using DAB+? It is more robust than DAB (7) Do primary overnight at 4 deg C and potentially increase primary concentration. I will try to give more comments once I see the picture, Andrea ----- Original Message ----- From: Ashley Haines Date: Friday, May 5, 2006 8:14 am Subject: [Histonet] texture/quality of DAB staining > > I am posting a picture called "fine DAB staining" > (http://www.histonet.org/site_images_frame.asp). I am hoping for > suggestions on how to improve the quality of the staining. In some > sensesit's a great result. You can see, at least under the scope > if not in the > posted micrograph, lots of fine detail. There are many fine > projectionsfrom the cell surface which stain positive. > Unfortunately, my goal is to > counterstain with a Wright Geimsa-like or Hema3 stain. The signal > I am > getting will not be visible with that counterstain. Can you > suggest any > ways to make my staining coarser and/or darker. Here's my basic > protocolfor the posted micrograph: > > > MeOH fix cytospin, block with 3% H202 in MeOH 1hr at RT, wash, > block with > 10% goat serum 1 hr, wash, incubate with biotinylated primary 1hr > at 37, > wash, incubate with SA-HRP (1:1000) for 1 hr at RT, wash, detect with > Vector's DAB substrate kit (with nickel enhancement) 20 min at RT, > wash in > DI. > > From peptolab <@t> hamptons.com Fri May 5 16:39:12 2006 From: peptolab <@t> hamptons.com (Jeff Silverman) Date: Fri May 5 16:40:04 2006 Subject: [Histonet] Pancreas markers Message-ID: <000501c6708c$5959d670$6401a8c0@jeffrey028c8d9> Pancreas specific markers?- no such thing but.. For ductal adenocarcinomas-MUC 1 is useful http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve &db=pubmed&dopt=Abstract&list_uids=14681945&query_hl=10&itool=pubmed_docsum For colloid carcinomas- MUC 2 helps http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve &db=pubmed&dopt=Abstract&list_uids=12717243&query_hl=10&itool=pubmed_docsum For islet cell neoplasia- chromogranin an or any of the possible hormones (insulin, gastrin, glucagon etc) Jeff Silverman HT HTL QIHC (ASCP) Pathologists' Assistant- Pathology Supervisor Southside Hospital North Shore Long Island Jewish Health System Bay Shore, New York USA From wood <@t> dcpah.msu.edu Sat May 6 21:43:22 2006 From: wood <@t> dcpah.msu.edu (Thomas Wood) Date: Sat May 6 21:43:40 2006 Subject: [Histonet] Paraffin scraper Message-ID: I use the long handled scrapper with an 8 inch blade and it really gouges the tiles if you are not extremely careful. Tom Wood Michigan State University Diagnostic Center for Population and Animal Health East Lansing, Mi From PKamalavenkatesh <@t> wockhardtin.com Sat May 6 22:26:54 2006 From: PKamalavenkatesh <@t> wockhardtin.com (PKamalavenkatesh@wockhardtin.com) Date: Sat May 6 22:21:03 2006 Subject: [Histonet] Re: BrdU staining Message-ID: Dear Shabana, Please refer the following article that will give you the best idea of BrdU staining as assessment of cell proliferation. Standardized assessment of cell proliferation: The approach of the RITA-CEPA working groups Thomas Noltea, Wolfgang Kaufmannb, Frederic Schorschc, Tony Soamesd, Edgar Webere Experimental and Toxicologic Pathology 57 (2005) 91?103 Regs Dr.P.Kamalavenkatesh Drug Discovery- Biology Pre clinical safety assessment division Wockhardt Research Center D-4, MIDC, Chikalthana Aurangabad India Please Visit our New Corporate Web Site www.wockhardt.com --------------------- Disclaimer ------------------------------------------------------------------------------ Information transmitted by this E-MAIL is proprietary to Wockhardt Ltd. and/ or its Group Companies and/or,its Customers and is intended for use only by the individual or entity to which it is addressed, and may contain information that is privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. --------------------------------------------------------------------------------------------------------------- From Terry.Marshall <@t> rothgen.nhs.uk Sun May 7 07:52:29 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Sun May 7 07:52:01 2006 Subject: [Histonet] Paraffin scraper Message-ID: I knew it would. Ho ho ho, we told you to use a hoe. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Thomas Wood [mailto:wood@dcpah.msu.edu] Sent: 07 May 2006 03:43 To: Histonet Histonet Subject: [Histonet] Paraffin scraper I use the long handled scrapper with an 8 inch blade and it really gouges the tiles if you are not extremely careful. Tom Wood Michigan State University Diagnostic Center for Population and Animal Health East Lansing, Mi _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun May 7 14:23:01 2006 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Sun May 7 14:23:26 2006 Subject: [Histonet] CD133 Message-ID: <200605071923.k47JN8VV078538@pro12.abac.com> Please if you have any positive results with this antibody, let me hear from you. This is supposed to be a marker for stem cells and maybe early endothelial cells in particular. I have only found one company that sells it. I am trying to get it to work on fetal baboon tissue. Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net website www.ihctech.net From AnthonyH <@t> chw.edu.au Sun May 7 19:05:50 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun May 7 19:06:10 2006 Subject: [Histonet] FW: H&E on Leica XL automatic stainer Message-ID: The following might be useful: Program 1 - Routine H&E Step Station Solution Time Exact 1 1 Xylene 4min N 2 2 Xylene 4min N 3 3 Ethanol 1min N 4 4 Ethanol 1min N 5 5 Ethanol 1min N 6 Wash 1 Water 1?min N 7 6 Haematoxylin 5min Y 8 Wash 2 Water 2min N 9 7 0.2% HCl 4sec Y 10 Wash 3 Water 1min N 11 8 Blueing 1min N 12 Wash 4 Water 1min N 13 9 Eosin 1min Y 14 10 Ethanol 30sec N 15 11 Ethanol 30sec N 16 12 Ethanol 30sec N 17 17 Xylene 1min N 18 18 Xylene 1min N 19 EXIT Xylene Station Solutions Station Solution Station Solution 1 Xylene 10 Ethanol 2 Xylene 11 Ethanol 3 Ethanol 12 Ethanol 4 Ethanol 13 Ethanol 5 Ethanol 14 Ethanol 6 Haematoxylin 15 Ethanol 7 0.2% HCl in water 16 Spare 8 Blueing 17 Xylene 9 Eosin 18 Xylene EXIT Xylene Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sanders, Julie, VHACIN Sent: Saturday, 6 May 2006 2:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: H&E on Leica XL automatic stainer -----Original Message----- From: Sanders, Julie, VHACIN Sent: Friday, May 05, 2006 11:28 AM To: histonet@lists.utsouthwestern.ed; 200/316 Unmatched Invoices Subject: H&E on Leica XL automatic stainer Hi Netters, I am trying to find different protocols for H&E on the Leica autostainer XL. I would appreciate anyone using this stainer sharing their procedure. Currently we are using Gills lll and Richard Allen Eosin...but I am interested in any/all procedures. Feel free to email them to me privately if you wish. Thanks in advance, Julie Julie A. Sanders, BA,HT(ASCP) Supervisor, Anatomic Pathology Cincinnati VAMC Julie.Sanders@va.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From john_paul1959 <@t> yahoo.com Sun May 7 22:03:17 2006 From: john_paul1959 <@t> yahoo.com (John Paul) Date: Sun May 7 22:03:23 2006 Subject: [Histonet] Re: Histonet Digest, Vol 30, Issue 8 Message-ID: <20060508030317.10743.qmail@web31311.mail.mud.yahoo.com> Hi everyone Can anyone recommend anti MMP-1, 2 and 9 antibodies that work well on formalin fixed paraffin embedded tissues? Thanks John histonet-request@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Job Opening (Luck, Greg D.) 2. Re: Microwave and DNA/Molecular (Johnson, Teri) 3. FW: MyNeuroLab.com Media (Charles Scouten) 4. Re: FW: H&E on Leica XL automatic stainer (mari.ann.mailhiot@leica-microsystems.com) 5. Re: counter-top tissue processors (Andrea Hooper) 6. Marker for invasive carcinoma (Jeff Silverman) 7. Re: texture/quality of DAB staining (Andrea Hooper) 8. Pancreas markers (Jeff Silverman) ---------------------------------------------------------------------- Message: 1 Date: Fri, 5 May 2006 10:22:19 -0700 From: "Luck, Greg D." Subject: [Histonet] Job Opening To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hello all, We have an histology position open for an HT or HTL certifed tech. Routine histology duties (embedding, cutting and special stains. IHC experience in addition preferred. This position is in a 300 bed community hospital in Spokane, WA. Below I have placed three weblinks for those interested so that they can go on the internet to look at our facility, corporate organization and the community and surrounding region. A great place to work and live. Please contact me directly. Thanks, Greg www.empirehealth.org www.deaconessmc.org www.visitspokane.com Greg Luck Anatomic Path Spvr Deaconess Med Cntr 800 W. 5th Ave Spokane, WA 99204 Offc 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org ------------------------------ Message: 2 Date: Fri, 5 May 2006 13:13:59 -0500 From: "Johnson, Teri" Subject: [Histonet] Re: Microwave and DNA/Molecular To: Message-ID: Content-Type: text/plain; charset="us-ascii" Doug, I think the jury is still out on that. Microwave radiation is non-ionizing electromagnetic energy similar to radar and sound waves. There have been some reports of state troopers getting tumors due to the radar guns, or folks having tumors from using cell phones. I don't know how good the science is behind it, but I'm thinking you'll find reports which state they do cause damage, and others which state they don't. If you're talking about what a microwave tissue processor would do to your DNA in the sample, I believe the radiation would not harm it. The molecules will vibrate in response to exposure to the microwaves and that is what generates the heat. Keeping the temperature controlled should keep the DNA from denaturing. High temperatures in the microwave, as those achieved doing HIER techniques, will surely cause DNA strands to separate. However, I suspect cooling should allow them to pair back up. But for routine processing, the heat never gets that high. I found information on this website (http://www.gallawa.com/microtech/mwave.html) which doesn't directly answer your question, but it does provide good information. Kok and Boon also have a book on Microwave technology you can buy through Milestone Medical. Good luck, Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64133 ------------------------------ Message: 3 Date: Fri, 5 May 2006 14:41:44 -0500 From: "Charles Scouten" Subject: [Histonet] FW: MyNeuroLab.com Media To: Message-ID: <5784D843593D874C93E9BADCB87342AB01306EE3@tpiserver03.Coretech-holdings.com> Content-Type: text/plain; charset="us-ascii" It sounds like she is making a Ralph knife for sectioning GMA/JB-4/HistOresin. Yes, these knife makers are still available. Glass knives are used extensively in EM labs but made in a different configuration, a triangled knife. It sounds like the knife breaker is in bad need of service. The knife scoreing wheel probably needs to be replaced, adjusted along with a few other replaceable parts. What model of glass breaker are you using? Lee Lee E. Dickey Product Marketing and Sales Manager McCormick Scientific ________________________________ From: Tabitha Granshaw [mailto:TGranshaw@biocompare.com] Sent: Wednesday, May 03, 2006 5:05 PM To: Charles Scouten Cc: Marla Steuer Subject: RE: MyNeuroLab.com Media Hi Charles, I just wanted to drop you a line to see if MyNeuroLab would be interested in a Promotion. This Promotion would be covered by the contract so there would be no additional charge. The Promotions can be viewed on the Biocompare site at http://www.biocompare.com/contents/9/Promotions-and-Special-Offers.html If you would be interested in setting one up, don't hesitate to drop me a line Cheers, Tabitha ________________________________ From: Charles Scouten [mailto:cwscouten@myneurolab.com] Sent: Friday, March 31, 2006 9:55 AM To: Tabitha Granshaw Subject: RE: MyNeuroLab.com Media Thanks, thats fine. Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com ________________________________ From: Tabitha Granshaw [mailto:TGranshaw@biocompare.com] Sent: Friday, March 31, 2006 11:08 AM To: Charles Scouten Cc: Marla Steuer Subject: RE: MyNeuroLab.com Media Good Morning Charles, The two sponsorship placements would be covered by the current contract, so there would be no additional charge. Since it sounds like the sponsorships are okay with you, I've gone ahead and scheduled them for: April 20 May 25 As the text I sent to you yesterday looks okay, I'll go ahead and place that in the two sponsorship spots. Drop me a line if you have any questions Cheers, Tabitha Tabitha Granshaw Manager of Media Coordination Biocompare The Buyer's Guide for Life Scientists 395 Oyster Point Blvd. Suite 405 South San Francisco, CA 94080 Phone (650) 416-0505 http://www.biocompare.com ________________________________ From: Charles Scouten [mailto:cwscouten@myneurolab.com] Sent: Friday, March 31, 2006 7:31 AM To: Tabitha Granshaw Subject: RE: MyNeuroLab.com Media yes, the attached text would be fine. I am a little confused, am I hereby authorizing a funds expenditure? I have not previosuly been in on what we do with these, and know we are short of cash these day. Sponsorship sounds like if costs us money. How much? Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com ________________________________ From: Tabitha Granshaw [mailto:TGranshaw@biocompare.com] Sent: Thursday, March 30, 2006 6:33 PM To: Charles Scouten Cc: Marla Steuer Subject: MyNeuroLab.com Media Hi Charles, Thanks for sending over the text for the New Technology - I've started the postig process for that and will let you know when it's live. Also, I'd like to schedule a couple Neuroscience sponsorships for MyNeuroLab. If you like we could schedule April 20 for MyNeuroLab.com, and perhaps also a date in May. Would the attached text be okay to run? (It's the text that was used for the most recent MyNeuroLab.com sponsorship) If you could let me know about this soon, that would be great as these spots go quickly Thanks Tabitha Tabitha Granshaw Manager of Media Coordination Biocompare The Buyer's Guide for Life Scientists 395 Oyster Point Blvd. Suite 405 South San Francisco, CA 94080 Phone (650) 416-0505 http://www.biocompare.com ------------------------------ Message: 4 Date: Fri, 5 May 2006 14:49:36 -0500 From: mari.ann.mailhiot@leica-microsystems.com Subject: Re: [Histonet] FW: H&E on Leica XL automatic stainer To: "Sanders, Julie, VHACIN" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Hi Julie I will be happy to send you some protocols for the AutoXL if you would like. Please let me know Best Regards Mari Ann Mailhiot BA HT ASCP Application Specialist Leica Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com "Sanders, Julie, VHACIN" .gov> Sent by: cc histonet-bounces@ lists.utsouthwest Subject ern.edu [Histonet] FW: H&E on Leica XL automatic stainer 05/05/2006 11:21 AM -----Original Message----- From: Sanders, Julie, VHACIN Sent: Friday, May 05, 2006 11:28 AM To: histonet@lists.utsouthwestern.ed; 200/316 Unmatched Invoices Subject: H&E on Leica XL automatic stainer Hi Netters, I am trying to find different protocols for H&E on the Leica autostainer XL. I would appreciate anyone using this stainer sharing their procedure. Currently we are using Gills lll and Richard Allen Eosin...but I am interested in any/all procedures. Feel free to email them to me privately if you wish. Thanks in advance, Julie Julie A. Sanders, BA,HT(ASCP) Supervisor, Anatomic Pathology Cincinnati VAMC Julie.Sanders@va.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ ------------------------------ Message: 5 Date: Fri, 05 May 2006 17:21:28 -0400 From: Andrea Hooper Subject: Re: [Histonet] counter-top tissue processors To: John Paul Cc: histonet@lists.utsouthwestern.edu Message-ID: <47ec5a7c1d205.445b8998@med.cornell.edu> Content-Type: text/plain; charset=us-ascii In the past I used the Leica TP1020 and found it to be *very* unsatisfactory - in fact we returned it and got an automated Leica TP1050 which was a dream to work with. I suggest you get a full sized processor as in my experience they are far superior. If you have cost concerns get a refurbished one but buy from a reliable instrument company. ----- Original Message ----- From: John Paul Date: Friday, May 5, 2006 9:56 am Subject: [Histonet] counter-top tissue processors > Hi everyone > We are looking to buy a counter-top (new or used) tissue > processor (paraffin) for a small research lab. I would appreciate > any recommendations from the group. > Have a great day!! > John > > > --------------------------------- > Yahoo! Mail goes everywhere you do. Get it on your phone. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 6 Date: Fri, 05 May 2006 17:28:37 -0400 From: Jeff Silverman Subject: [Histonet] Marker for invasive carcinoma To: bliven.laura@marshfieldclinic.org, histonet@lists.utsouthwestern.edu Message-ID: <000001c6708a$df34b910$6401a8c0@jeffrey028c8d9> Content-Type: text/plain; charset=us-ascii Laura, Finally, my research interest is finding some use in practice :-). In the breast and in many other organs, the normal resting periductal, perilobular, and interstitial fibroblasts express CD34. As carcinomatous invasion occurs, resting CD34+ dendritic stromal fibroblasts transform into actin+ myofibroblasts so analysis of these two stromal markers are useful in evaluating the presence or absence of carcinomatous invasion. Double stains for cytokeratin and alpha smooth muscle actin have been used to demonstrate the presence of invasion ie carcinoma cells surrounded by or amidst myofibroblasts. These contractile cells are necessary to effect migration through the connective tissue. The actin antibody will also detect the presence or absence of myoepithelial cells in a given lesion. http://www.blackwell-synergy.com/links/doi/10.1046%2Fj.1365-2559.1996.d01-51 0.x http://jcp.bmjjournals.com/cgi/content/full/56/4/271 This CD34 versus smooth muscle actin approach was recently reported useful in evaluating invasive implants of peritoneal mesothelioma versus benign endosalpingiosis in omentum and peritoneum. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve bstract&list_uids=16415795&query_hl=1&itool=pubmed_docsum> &db=pubmed&dopt=Abstract&list_uids=16415795&query_hl=1&itool=pubmed_docsum Hope this helps. Jeff Silverman HT HTL QIHC (ASCP) Pathologists' Assistant- Pathology Supervisor Southside Hospital North Shore Long Island Jewish Health System Bay Shore, New York USA ------------------------------ Message: 7 Date: Fri, 05 May 2006 17:34:14 -0400 From: Andrea Hooper Subject: Re: [Histonet] texture/quality of DAB staining To: Ashley Haines Cc: Histonet@lists.utsouthwestern.edu Message-ID: <48ca4e7d1ed65.445b8c96@med.cornell.edu> Content-Type: text/plain; charset=us-ascii Hi Ashley, Your pic isn't on the site yet so I haven't been able to see precisely what you are talking about but I have a few suggestions to increase staining based on your protocol: (1) I am assuming from your protocol and desire to counterstain with WG that you are doing cytospins of fresh cells???? (2) Did you try other fixations besides methanol? I find that methanol sometimes can reduce staining for certain antigens? (3) Use 0.3% H202 in H20 for 10 minutes as 3% is harsh for cytospins or fresh tissue. (4) Add another amplification step such as using a primary, biotinylated secondary and then Strep-HRP. Using a directly labeled primary will reduce your overall amplification. (5) You could try Vector's ABC instead of Strep-HRP, your staining will be more intense though be careful for background (6) Are you using DAB+? It is more robust than DAB (7) Do primary overnight at 4 deg C and potentially increase primary concentration. I will try to give more comments once I see the picture, Andrea ----- Original Message ----- From: Ashley Haines Date: Friday, May 5, 2006 8:14 am Subject: [Histonet] texture/quality of DAB staining > > I am posting a picture called "fine DAB staining" > (http://www.histonet.org/site_images_frame.asp). I am hoping for > suggestions on how to improve the quality of the staining. In some > sensesit's a great result. You can see, at least under the scope > if not in the > posted micrograph, lots of fine detail. There are many fine > projectionsfrom the cell surface which stain positive. > Unfortunately, my goal is to > counterstain with a Wright Geimsa-like or Hema3 stain. The signal > I am > getting will not be visible with that counterstain. Can you > suggest any > ways to make my staining coarser and/or darker. Here's my basic > protocolfor the posted micrograph: > > > MeOH fix cytospin, block with 3% H202 in MeOH 1hr at RT, wash, > block with > 10% goat serum 1 hr, wash, incubate with biotinylated primary 1hr > at 37, > wash, incubate with SA-HRP (1:1000) for 1 hr at RT, wash, detect with > Vector's DAB substrate kit (with nickel enhancement) 20 min at RT, > wash in > DI. > > ------------------------------ Message: 8 Date: Fri, 05 May 2006 17:39:12 -0400 From: Jeff Silverman Subject: [Histonet] Pancreas markers To: Jackie.O'Connor@abbott.com, histonet@lists.utsouthwestern.edu Message-ID: <000501c6708c$5959d670$6401a8c0@jeffrey028c8d9> Content-Type: text/plain; charset=us-ascii Pancreas specific markers?- no such thing but.. For ductal adenocarcinomas-MUC 1 is useful http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve bstract&list_uids=14681945&query_hl=10&itool=pubmed_docsum> &db=pubmed&dopt=Abstract&list_uids=14681945&query_hl=10&itool=pubmed_docsum For colloid carcinomas- MUC 2 helps http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve bstract&list_uids=12717243&query_hl=10&itool=pubmed_docsum> &db=pubmed&dopt=Abstract&list_uids=12717243&query_hl=10&itool=pubmed_docsum For islet cell neoplasia- chromogranin an or any of the possible hormones (insulin, gastrin, glucagon etc) Jeff Silverman HT HTL QIHC (ASCP) Pathologists' Assistant- Pathology Supervisor Southside Hospital North Shore Long Island Jewish Health System Bay Shore, New York USA ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 30, Issue 8 *************************************** --------------------------------- Get amazing travel prices for air and hotel in one click on Yahoo! FareChase From kemlo.rogerson <@t> waht.swest.nhs.uk Mon May 8 02:14:30 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon May 8 02:13:28 2006 Subject: [Histonet] Paraffin scraper Message-ID: You know you try your best to teach the Colonials, but they don't listen! Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 The greater our awareness of intentions, the greater our freedom to choose. -- Gil Fronsdal This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: Sunday, May 07, 2006 1:52 PM To: Thomas Wood; Histonet Histonet Subject: RE: [Histonet] Paraffin scraper I knew it would. Ho ho ho, we told you to use a hoe. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Thomas Wood [mailto:wood@dcpah.msu.edu] Sent: 07 May 2006 03:43 To: Histonet Histonet Subject: [Histonet] Paraffin scraper I use the long handled scrapper with an 8 inch blade and it really gouges the tiles if you are not extremely careful. Tom Wood Michigan State University Diagnostic Center for Population and Animal Health East Lansing, Mi _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Mon May 8 02:53:52 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon May 8 02:54:00 2006 Subject: [Histonet] Paraffin scraper Message-ID: Oh, No time to learn. We're too busy playing Cricket & Rugby!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: Monday, 8 May 2006 5:15 PM To: 'Marshall Terry Dr, Consultant Histopathologist'; Thomas Wood; Histonet Histonet Subject: RE: [Histonet] Paraffin scraper You know you try your best to teach the Colonials, but they don't listen! Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 The greater our awareness of intentions, the greater our freedom to choose. -- Gil Fronsdal This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: Sunday, May 07, 2006 1:52 PM To: Thomas Wood; Histonet Histonet Subject: RE: [Histonet] Paraffin scraper I knew it would. Ho ho ho, we told you to use a hoe. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Thomas Wood [mailto:wood@dcpah.msu.edu] Sent: 07 May 2006 03:43 To: Histonet Histonet Subject: [Histonet] Paraffin scraper I use the long handled scrapper with an 8 inch blade and it really gouges the tiles if you are not extremely careful. Tom Wood Michigan State University Diagnostic Center for Population and Animal Health East Lansing, Mi _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From mikael.niku <@t> helsinki.fi Mon May 8 04:30:12 2006 From: mikael.niku <@t> helsinki.fi (Mikael Niku) Date: Mon May 8 04:30:23 2006 Subject: [Histonet] Microwave and DNA/Molecular In-Reply-To: <3F500F8B416C554EBB21FF16642F72E959CE73@marxchg03.mar.med.navy.mil> References: <3F500F8B416C554EBB21FF16642F72E959CE73@marxchg03.mar.med.navy.mil> Message-ID: <445F0FA4.2020807@helsinki.fi> Hello Douglas! We are routinely using microwave heating to dramatically enhance in situ hybridization to genomic DNA in paraffin-embedded sections of PFA-fixed tissue material. This is like a standard antigen retrieval treatment (15 minutes in a boiling buffer solution, then 20 min slow cooling). The results are much better than without microwave heating. Please note we still need to do a separate denaturation step to facilitate hybridization, so it seems the DNA is not at least permanently denatured. I think we are also doing succesful PCR in situ hybridization in the same way, although this method is not in my personal use. So, at this scale there doesn't seem to be harm done. But I have never isolated DNA from samples processed this way, and I guess any other kind of molecular testing would need to be tried and confirmed separately. With best regards, Mikael Deltour, Douglas D. (HM2) wrote: > Hello, > > > > I am looking for some information that can confirm or deny that microwave > technology would degrade DNA and limit molecular testing. Thanks! > > -- //////////////////////////////////////////////////////////// Mikael Niku URL: www.helsinki.fi/~mniku/ University of Helsinki Dept. Basic Veterinary Sciences - Mit?k? mielt? olen l?nsimaisesta sivistyksest?? Minusta se olisi erinomainen ajatus! - Gandhi //////////////////////////////////////////////////////////// From mstahl <@t> bvha.org Mon May 8 06:32:49 2006 From: mstahl <@t> bvha.org (Stahl, Michael) Date: Mon May 8 06:32:28 2006 Subject: [Histonet] Paraffin scraper Message-ID: <4C878E714B21EB4F8EB159777B8822EE5B680B@bvfyms01.net.bvha.org> Well this colonial has learned not to spill paraffin (grins) In my rookie years, I just used a cheap plastic putty knife Michael P.Stahl HT(ASCP) BVRHC Findlay, Ohio 45840 From mgdelaware <@t> comcast.net Mon May 8 08:54:48 2006 From: mgdelaware <@t> comcast.net (Marian Powers) Date: Mon May 8 08:54:52 2006 Subject: [Histonet] Part-time position Message-ID: <000b01c672a6$f8cefc50$3227c847@D7XQNX91> Looking for an experienced Histotech. to work part-time in Dover, Delaware. Marian Powers, HT(ASCP) (302)-677-0000 mpowers@dpspa.com From srishan <@t> mail.holyname.org Mon May 8 10:20:09 2006 From: srishan <@t> mail.holyname.org (srishan@mail.holyname.org) Date: Mon May 8 10:20:30 2006 Subject: [Histonet] Fw: 0.3% Eriochrome Black T. C.I.14645 Message-ID: ----- Forwarded by Nirmala Srishan/HNH on 05/08/2006 11:17 AM ----- Nirmala Srishan/HNH To 05/08/2006 11:11 histonet@lists.utsouthwestern.edu AM cc Subject 0.3% Eriochrome Black T. C.I.14645 Hi, In reference to the artical from Journal of Histotechnology, March 2006. auramine O-rgidamine B solution used along with the counter stain 0.3% Eriochrome Black T solution is preferable. We would like to do a comparison study. Does any one knows who carries Eiochrome Black T. Any information is appreciated. Thanks Nirmala Srishan Histology Supervisor Holy Name Hospital Teaneck NJ 07666 From Jackie.O'Connor <@t> abbott.com Mon May 8 11:24:45 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Mon May 8 11:25:13 2006 Subject: [Histonet] FSGO In-Reply-To: <001f01c66fcd$a0b0d4c0$0300a8c0@Chlipala> Message-ID: For IHC purposes, is this Fish skin gelatin oil? I have an IHC protocol which recommends using FSGO in PBS, although I'm not sure what it is or what it does. If you do, let me know. Jackie O' From galinadeyneko <@t> yahoo.com Mon May 8 13:09:54 2006 From: galinadeyneko <@t> yahoo.com (Galina Deyneko) Date: Mon May 8 13:53:34 2006 Subject: [Histonet] Subject: wheat germ agglutinin Message-ID: <20060508180954.20034.qmail@web33113.mail.mud.yahoo.com> Dear colleagues, I would like to ask some advice regarding WGA. We would like to use WGA for label cardiomyocytes membranes on FFPE mouse hearts for image analysis.I clearly remember a message from Dr. J.Kiernan and agree with his opinion, but my lab head would like to try. I stained cardiomyocytes with biotynilated WGA from Vector with ABC kit and received strong signal from endothelial cells and weak signal on cell membrane (I placed picture on histonet web). Now my lab head would like to try with FITC WGA (Vector). Please, share with me protocols, any prompts will appreciated. In archive I found a lot of questions, but no answers. What dilution or final concentration do you recommend? What I should use for blocking- sugar or serum or both? What I should use as a diluent? vector recommends 1% BSA/PBS, book "Lectin histochemistry" recommended by Gayle , as well as J.Kiernan recommend to use lectin buffer (Tris with some salts). May be I should use other fluorescent label? I will appreciate any advices. Protocol for biotynilated WGA which I used: Block of peroxidase Block with 2% BSA/PBS WGA dilution 1:250 (20 ug/ml final concentration) with 2%BSA/PBS Incubation 1 hour at RT in humid chamber Developing with ABC kit (Vector) for 30 min and DAB for 5 min No counterstaining. I did not describe routine de-wax, wash in PBS ect. Thank you. galina deyneko Novartis Cambridge M6170871-7613. --------------------------------- How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. From gcallis <@t> montana.edu Mon May 8 14:20:05 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon May 8 14:20:12 2006 Subject: methanol fixation for cell surface marker, the little experiment Re: [Histonet] texture/quality of DAB staining In-Reply-To: <48ca4e7d1ed65.445b8c96@med.cornell.edu> References: <48ca4e7d1ed65.445b8c96@med.cornell.edu> Message-ID: <6.0.0.22.1.20060508130932.01b1ee90@gemini.msu.montana.edu> I agree whole heartedly with Andrea on using methanol for fixation. We did a little experiment using Methanol Cold acetone 4C 10 min Acetone 75%/absolute ethanol 25% for 5 min at RT We did a Phase 2 Coxiella bacteria, immunofluorescent staining using Alexa 488 as fluorophore. The staining for both had obvious, dimished intensity after methanol fixation Acetone for 10 min 4C came in second with diminished intensity, but not as bad as the methanol fixation Acetone/alcohol mixture was decidely the winner with far more cells staining and also bacteria. It is always a good idea to try different fixations. Caveats about using methanol fixation are published by Elias book, have also seen this in other literature - that methanol fixation will compromise cell surface staining, i.e. CD markers - and this alcohol is probably performing some protein hydrolysis which can destroy antigens. We never use it for endogenous peroxidase blocking either, we put our hydrogen peroxide in buffer rather than methanol. At 03:34 PM 5/5/2006, you wrote: >(2) Did you try other fixations besides methanol? I find that methanol >sometimes can reduce staining for certain >antigens? Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From gpbnas <@t> yahoo.es Mon May 8 14:24:24 2006 From: gpbnas <@t> yahoo.es (Guillermo Palao) Date: Mon May 8 14:24:36 2006 Subject: [Histonet] Hematoxylin staining of frozen samples, where are the nuclei? NUCLEI FOUND! In-Reply-To: <6.0.0.22.1.20060404154036.01b69f38@gemini.msu.montana.edu> Message-ID: <20060508192424.1983.qmail@web26201.mail.ukl.yahoo.com> Thanks Gayle for your great advice! I left frozen sections for a few weeks but I recently came back to them and put in practice your suggestion with great results. Nuclei now look really nice. I have heard this is a fairly common problem, so I hope more people will benefit from this great tip. Best regards, Guillermo Gayle Callis escribi?: Guillermo It is because they are minimally fixed with acetone, correct? The nuclei are not lost, just look funny like bubble with blue rim or vacuoles with blue rims. I know the problem. A lot of the problem is caused by the kind of water you rinse with to stop the chromogen reaction - distilled water is not ideal. Chris van der Loos has the answer for this by post fixing an IHC stained frozen section with formalin before doing the hematoxylin staining. For further discussion, you can contact him at "C.M. vander Loos" . This was discussed in the past year at great length by him and others on Histonet. This post fixation with formalin seems to restore and fix the nuclei - it is important to remember, acetone is a precipitating fixative and this is very minimal as compared to fixing a frozen section with NBF or paraformaldehyde, cross linking fixatives. How he does this: Do all your IHC staining up through completion of chromogen step, and after final rinse (do not use distilled water, tap water or even PBS may be better) then: Postfix in neutral buffered formalin for 3 minutes Rinse 2 min with gently running tap water, Brief rinse in distilled water Do the Hematoxylin counterstain See if this helps you At 03:22 PM 4/4/2006, you wrote: >Dear histonetters, > > When I stain samples placed on VWR frosted slides with Gills > hematoxylin, nuclei are nicely stained. However, when the same samples > are counterstained after passing through all necessary steps involved in > immunohistochemistry, hematoxylin staining of nuclei is simply horrible. > Is it possible that nuclei are lost in the different washing steps? > Please put forward any suggestions you might have. > > > > >--------------------------------- > >LLama Gratis a cualquier PC del Mundo. >Llamadas a fijos y m?viles desde 1 c?ntimo por minuto. >http://es.voice.yahoo.com >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) Guillermo Palao, MD. Ph.D. Laboratorio de Reumatolog?a Centro de Investigaci?n Hospital 12 de Octubre Avda de C?rdoba s/n Madrid 28041 Spain --------------------------------- LLama Gratis a cualquier PC del Mundo. Llamadas a fijos y m?viles desde 1 c?ntimo por minuto. http://es.voice.yahoo.com From carl.hobbs <@t> kcl.ac.uk Mon May 8 14:28:25 2006 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Mon May 8 14:29:00 2006 Subject: [Histonet] Re CD133 Message-ID: Interestingly/frustratingly I used Abcam's anti CD133 on mouse /rat FFPW sections to see what would happen, as I am also interested in stem cells. I have only ever got positivity in rat ependymal cells, after HIER. Haven't tried any other species..... Please look in the picture gallery ( type in CD133 in the search box) here, http://www.immunoportal.com/ if you wish. I would be interested in any further comments too..... Carl From gcallis <@t> montana.edu Mon May 8 14:36:13 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon May 8 14:36:21 2006 Subject: [Histonet] FSGO In-Reply-To: References: <001f01c66fcd$a0b0d4c0$0300a8c0@Chlipala> Message-ID: <6.0.0.22.1.20060508133101.01b51540@gemini.msu.montana.edu> I used to have fish gelatin, a green gooey super thick liquid purchased from Sigma but it was not oily. I have not used it for routine IHC non serum blocking step in lieu of BSA, a normal serum or casein blocking but some people in EM swear by it. You may want to check up on some immuno-electron microscopy methods, that is where I have seen it used. I know it was discussed many years ago on Histonet, a keyword search may bring it up. Fish gelatin rather than fish skin gelatin. 10:24 AM 5/8/2006, you wrote: >For IHC purposes, is this Fish skin gelatin oil? I have an IHC protocol >which recommends using FSGO in PBS, although I'm not sure what it is or >what it does. >If you do, let me know. > >Jackie O' Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From ROrr <@t> enh.org Mon May 8 15:01:20 2006 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Mon May 8 15:01:25 2006 Subject: [Histonet] Remstar Message-ID: Hi Everyone, My Managers just came back for the Executive War College in Miami. Evidently there was a display with the Remstar storage system. I know these have been used in industry, but does anyone have this system in use for storing archived slides or even supplies for Anatomic Pathology? This storage system is supposed to save over 60% space currently used for storing surgical slides. Pro and Con comments appreciated. Thanks a bunch! Becky Becky Orr CLA,HT(ASCP) Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 From jkiernan <@t> uwo.ca Mon May 8 15:04:28 2006 From: jkiernan <@t> uwo.ca (John A. Kiernan) Date: Mon May 8 15:04:19 2006 Subject: [Histonet] Subject: wheat germ agglutinin References: <20060508180954.20034.qmail@web33113.mail.mud.yahoo.com> Message-ID: <445FA44C.679C0096@uwo.ca> Serum albumin is a glycoprotein, so it doesn't make good sense to include it in a solution of a lectin. Go with Brooks, Leathen & Schumacher's "Lectin Histochemistry", which is an authoritative source and gives very detailed, easily followed instructions. As you have noted, they recommend (p.159) a TRIS buffer with a little Ca and Mg chlorides added. (For some lectins it's Mn rather than Mg.) The only thing that's likely to need adjustment is the concentration of the labelled lectin. When I worked with fluorescently labelled lectins I preferred a rhodamine to a fluorescein label because autofluorescence was less of an annoyance. You can, however, suppress all autofluorescence before staining. See Biotech. Histochem. 77(4): 232 (2002). -- ------------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan[AT]uwo.ca http://publish.uwo.ca/~jkiernan/ http://instruct.uwo.ca/anatomy/530/index.htm _______________________________ Galina Deyneko wrote: > > Dear colleagues, > I would like to ask some advice regarding WGA. > We would like to use WGA for label cardiomyocytes membranes on FFPE mouse hearts for image analysis.I clearly remember a message from Dr. J.Kiernan and agree with his opinion, but my lab head would like to try. I stained cardiomyocytes with biotynilated WGA from Vector with ABC kit and received strong signal from endothelial cells and weak signal on cell membrane (I placed picture on histonet web). Now my lab head would like to try with FITC WGA (Vector). Please, share with me protocols, any prompts will appreciated. In archive I found a lot of questions, but no answers. What dilution or final concentration do you recommend? What I should use for blocking- sugar or serum or both? What I should use as a diluent? vector recommends 1% BSA/PBS, book "Lectin histochemistry" recommended by Gayle , as well as J.Kiernan recommend to use lectin buffer (Tris with some salts). May be I should use other fluorescent label? > I will appreciate any advices. > Protocol for biotynilated WGA which I used: > Block of peroxidase > Block with 2% BSA/PBS > WGA dilution 1:250 (20 ug/ml final concentration) with 2%BSA/PBS > Incubation 1 hour at RT in humid chamber > Developing with ABC kit (Vector) for 30 min and DAB for 5 min > No counterstaining. > I did not describe routine de-wax, wash in PBS ect. > Thank you. > galina deyneko > Novartis Cambridge M6170871-7613. > > > --------------------------------- > How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Mon May 8 15:21:11 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon May 8 15:21:16 2006 Subject: [Histonet] Subject: wheat germ agglutinin In-Reply-To: <20060508180954.20034.qmail@web33113.mail.mud.yahoo.com> References: <20060508180954.20034.qmail@web33113.mail.mud.yahoo.com> Message-ID: <6.0.0.22.1.20060508133818.01b8db48@gemini.msu.montana.edu> Lectins are notorious for binding to more than one tissue component containing the carbohydrate residue the lectin binds to. Be sure to look up what WGA binds to in order to know if you are going to have more than one structure, other than the cardiomyocytes being stained. At 12:09 PM 5/8/2006, you wrote: >Dear colleagues, > I would like to ask some advice regarding WGA. > We would like to use WGA for label cardiomyocytes membranes on FFPE > mouse hearts for image analysis.I clearly remember a message from Dr. > J.Kiernan and agree with his opinion, but my lab head would like to try. > I stained cardiomyocytes with biotynilated WGA from Vector with ABC kit > and received strong signal from endothelial cells and weak signal on cell > membrane (I placed picture on histonet web). To try fluorescent staining, use the WGA-biotinylated, and come back with Molecular Probes Strepavidin- Alexa 488 or 555. but use avidin/biotin blocking to quench any endogenous biotin present. A correct negative control is the inhibiting/eluting sugar 500mMNacetylglucosamine, chitin hydrolysate from Vector, or 100 mM acetic acid. The sugar is what we use with lectins, and you take your working concentration of WGA, and dilute it in the 500 mM N acetylglucosamine, let it incubate for 30 min to 1 hour (we let it incubate overnight at 4C to ensure a lectin is totally bound to the sugar and not available to bind to the carbohydrate residue in the cardiomyocytes. This is added to a section as a negative control. >Now my lab head would like to try with FITC WGA (Vector). Please, share >with me protocols, any prompts will appreciated. In archive I found a lot >of questions, but no answers. What dilution or final concentration do you >recommend? Do a dilution panel, starting at 40 ug/ml then 20 ug/ml, then 10 ug/ml, 5 ug/ml, etc, etc. 30 minutes should work for incubation. >What I should use for blocking- sugar or serum or both? Never use serum with lectins, there are carbohydrate residues in serums that will bind to the lectin. There is no reason to block with lectins, they are NOT antibodies. However if you work with biotinylated lectins, Strepavidin or an ABC system, you should do an avidin/biotin block to quench endogenous biotin present. >What I should use as a diluent? The rinse buffer recommended by Vector is sufficient. >Vector recommends 1% BSA/PBS, book "Lectin histochemistry" recommended by >Gayle , as well as J.Kiernan recommend to use lectin buffer (Tris with >some salts). >What John referred to IS the lectin buffer which is: 10X Lectin Buffer Tris 60.57 g NaCl 87 g 2.03 g Magnesium chloride 1.11 g calcium chloride in 1 liter distilled water, adjust pH to 7.6 with Concentrated HCl. Working buffer is either bring this to final volume of 10 L with distilled water or dilute the 10X buffer 1:10 to make working Lectin buffer. This buffer is simply a routine TBS buffer with calcium chloride and magnesium chloride added. If Vector recommends 1% BSA/PBS then use it, but remember that some lectins will not work well with phosphate ions present, hence the lectin buffer recommendation. WGA and UEA1 are NOT affected by phosphate ions, so what Vector recommends should work well. >May be I should use other fluorescent label? WGA conjugated to FITC should work well, we had UEA1-FITC direct staining on M cells in small intestine frozen sections. The section can be mounted with VECTASHIELD hardset containing DAPI, but for imaging, VECTASHIELD hard set alone. I will appreciate any advices. > Protocol for biotynilated WGA which I used: > Block of peroxidase Yes with a peroxidase system > Block with 2% BSA/PBS You are not performing an antibody stain, a protein block is not needed for lectins > WGA dilution 1:250 (20 ug/ml final concentration) with 2%BSA/PBS > Incubation 1 hour at RT in humid chamber > Developing with ABC kit (Vector) for 30 min and DAB for 5 min > No counterstaining. > I did not describe routine de-wax, wash in PBS ect. If you have poor staining, weak, etc - a trypsin enzyme digestion for 10 min at 37C will work on FFPE tissues. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From gcallis <@t> montana.edu Mon May 8 15:24:16 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon May 8 15:24:20 2006 Subject: [Histonet] Hematoxylin staining of frozen samples, where are the nuclei? NUCLEI FOUND! In-Reply-To: <20060508192424.1983.qmail@web26201.mail.ukl.yahoo.com> References: <6.0.0.22.1.20060404154036.01b69f38@gemini.msu.montana.edu> <20060508192424.1983.qmail@web26201.mail.ukl.yahoo.com> Message-ID: <6.0.0.22.1.20060508142130.01b4a3e8@gemini.msu.montana.edu> Guillemo, Hooray for success. This is a technic I learned from Dr. Chris van der Loos, he needs a round of applause, I just passed on his solution to the problem. Gayle Callis I think what is most important is that At 01:24 PM 5/8/2006, you wrote: >Thanks Gayle for your great advice! I left frozen sections for a few weeks >but I recently came back to them and put in practice your suggestion with >great results. > >Nuclei now look really nice. I have heard this is a fairly common problem, >so I hope more people will benefit from this great tip. > >Best regards, > >Guillermo > >Gayle Callis escribi?: >Guillermo > >It is because they are minimally fixed with acetone, correct? The nuclei >are not lost, just look funny like bubble with blue rim or vacuoles with >blue rims. I know the problem. A lot of the problem is caused by the kind >of water you rinse with to stop the chromogen reaction - distilled water is >not ideal. > >Chris van der Loos has the answer for this by post fixing an IHC stained >frozen section with formalin before doing the hematoxylin staining. For >further discussion, you can contact him at "C.M. vander Loos" >. This was discussed in the past year at great >length by him and others on Histonet. This post fixation with formalin >seems to restore and fix the nuclei - it is important to remember, acetone >is a precipitating fixative and this is very minimal as compared to fixing >a frozen section with NBF or paraformaldehyde, cross linking fixatives. > >How he does this: > >Do all your IHC staining up through completion of chromogen step, and after >final rinse (do not use distilled water, tap water or even PBS may be >better) then: > >Postfix in neutral buffered formalin for 3 minutes >Rinse 2 min with gently running tap water, >Brief rinse in distilled water >Do the Hematoxylin counterstain > >See if this helps you > > > > > >At 03:22 PM 4/4/2006, you wrote: > >Dear histonetters, > > > > When I stain samples placed on VWR frosted slides with Gills > > hematoxylin, nuclei are nicely stained. However, when the same samples > > are counterstained after passing through all necessary steps involved in > > immunohistochemistry, hematoxylin staining of nuclei is simply horrible. > > Is it possible that nuclei are lost in the different washing steps? > > Please put forward any suggestions you might have. > > > > > > > > > >--------------------------------- > > > >LLama Gratis a cualquier PC del Mundo. > >Llamadas a fijos y m?viles desde 1 c?ntimo por minuto. > >http://es.voice.yahoo.com > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >Gayle Callis >MT,HT,HTL(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University - Bozeman >PO Box 173610 >Bozeman MT 59717-3610 >406 994-6367 >406 994-4303 (FAX) > > > > > >Guillermo Palao, MD. Ph.D. >Laboratorio de Reumatolog?a >Centro de Investigaci?n >Hospital 12 de Octubre >Avda de C?rdoba s/n >Madrid 28041 >Spain > > > >LLama Gratis a cualquier PC del Mundo. >Llamadas a fijos y m?viles desde 1 c?ntimo por minuto. >http://es.voice.yahoo.com > From mrcraigwbarlow <@t> googlemail.com Tue May 9 07:44:57 2006 From: mrcraigwbarlow <@t> googlemail.com (CRAIG BARLOW) Date: Tue May 9 07:45:03 2006 Subject: [Histonet] anti-Rat endothelial cell marker for ffpe tissue Message-ID: <22482b560605090544w3cefbeddq44928be6bd0de73@mail.gmail.com> Hello all, I am just wondering if anybody could reccomend an anti-rat endothelial cell antibody that will work in FFPE tissue and protocol if poss. I would like to stain all the vessels in the skin to look at angiogenesis. Any help will be welcome. Regards CRaig From la.sebree <@t> hosp.wisc.edu Tue May 9 08:13:23 2006 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Tue May 9 08:13:32 2006 Subject: [Histonet] D2-40 antibody Message-ID: One of our residents read about this antibody against lymphatic endothelium and wondered if its known by another name. Any help out there? Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From juan.gutierrez <@t> christushealth.org Tue May 9 09:37:29 2006 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Tue May 9 09:37:44 2006 Subject: [Histonet] D2-40 antibody Message-ID: Try Signet Labs. Catalog # 730 1-800-223-0796 or www.signetlabs.com Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A. Sent: Tuesday, May 09, 2006 8:13 AM To: Histonet Subject: [Histonet] D2-40 antibody One of our residents read about this antibody against lymphatic endothelium and wondered if its known by another name. Any help out there? Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DavidH <@t> marketlabinc.com Tue May 9 10:09:56 2006 From: DavidH <@t> marketlabinc.com (David Haagsma) Date: Tue May 9 10:10:00 2006 Subject: [Histonet] Thermo and Fisher Scientific to Merge Message-ID: <19E3602A16438E48B51A4250CA04B5F66451FE@exchange.marketlab.com> Has anybody seen this yet?... >From Thermo's site... Learn about Thermo Electron and Fisher Scientific combining to create the world's leading provider of laboratory products and services in the life, laboratory and health sciences industry. When the merger is completed,?Thermo Fisher Scientific will offer offer complementary technologies, life science consumables, software and services to provide more complete and integrated solutions with an industry-leading global sales and service organization of nearly 7,500 professionals worldwide. Dave Haagsma MT Research Market Manager MarketLab Inc. From DDDeltour <@t> mar.med.navy.mil Tue May 9 10:25:05 2006 From: DDDeltour <@t> mar.med.navy.mil (Deltour, Douglas D. (HM2)) Date: Tue May 9 10:25:34 2006 Subject: [Histonet] Thermo and Fisher Scientific to Merge Message-ID: <3F500F8B416C554EBB21FF16642F72E959CE7D@marxchg03.mar.med.navy.mil> Thermo-Shandon-Electron-Fisher Scientific. Anyone else want to join in? :) -----Original Message----- From: David Haagsma [mailto:DavidH@marketlabinc.com] Sent: Tuesday, May 09, 2006 11:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thermo and Fisher Scientific to Merge Has anybody seen this yet?... >From Thermo's site... Learn about Thermo Electron and Fisher Scientific combining to create the world's leading provider of laboratory products and services in the life, laboratory and health sciences industry. When the merger is completed,?Thermo Fisher Scientific will offer offer complementary technologies, life science consumables, software and services to provide more complete and integrated solutions with an industry-leading global sales and service organization of nearly 7,500 professionals worldwide. Dave Haagsma MT Research Market Manager MarketLab Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From juan.gutierrez <@t> christushealth.org Tue May 9 10:49:36 2006 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Tue May 9 10:49:46 2006 Subject: [Histonet] Thermo and Fisher Scientific to Merge Message-ID: Thermo-Electron-Shandon-Lipshaw-Fisher-Richard Allan-Scientific or Apogent for short. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Deltour, Douglas D. (HM2) Sent: Tuesday, May 09, 2006 10:25 AM To: 'David Haagsma'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Thermo and Fisher Scientific to Merge Thermo-Shandon-Electron-Fisher Scientific. Anyone else want to join in? :) -----Original Message----- From: David Haagsma [mailto:DavidH@marketlabinc.com] Sent: Tuesday, May 09, 2006 11:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thermo and Fisher Scientific to Merge Has anybody seen this yet?... >From Thermo's site... Learn about Thermo Electron and Fisher Scientific combining to create the world's leading provider of laboratory products and services in the life, laboratory and health sciences industry. When the merger is completed,?Thermo Fisher Scientific will offer offer complementary technologies, life science consumables, software and services to provide more complete and integrated solutions with an industry-leading global sales and service organization of nearly 7,500 professionals worldwide. Dave Haagsma MT Research Market Manager MarketLab Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Tue May 9 10:52:30 2006 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Tue May 9 10:52:38 2006 Subject: [Histonet] Thermo and Fisher Scientific to Merge Message-ID: Thermoshandonelectronfisherenronnocompetitionhigherpricesmoreprofitsscientific?? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Deltour, Douglas D. (HM2) Sent: 09 May 2006 16:25 To: 'David Haagsma'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Thermo and Fisher Scientific to Merge Thermo-Shandon-Electron-Fisher Scientific Anyone else want to join in? :) -----Original Message----- From: David Haagsma [mailto:DavidH@marketlabinc.com] Sent: Tuesday, May 09, 2006 11:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thermo and Fisher Scientific to Merge Has anybody seen this yet?... >From Thermo's site... Learn about Thermo Electron and Fisher Scientific combining to create the world's leading provider of laboratory products and services in the life, laboratory and health sciences industry. When the merger is completed,?Thermo Fisher Scientific will offer offer complementary technologies, life science consumables, software and services to provide more complete and integrated solutions with an industry-leading global sales and service organization of nearly 7,500 professionals worldwide. Dave Haagsma MT Research Market Manager MarketLab Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From STapper <@t> slhduluth.com Tue May 9 10:52:31 2006 From: STapper <@t> slhduluth.com (Tapper, Sheila) Date: Tue May 9 10:52:41 2006 Subject: [Histonet] D2-40 antibody Message-ID: Dako carries this antibody. I have seen it in literature called podoplanin. (sp?) Sheila Tapper HT(ASCP) St. Luke's Hospital Duluth, MN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A. Sent: Tuesday, May 09, 2006 8:13 AM To: Histonet Subject: [Histonet] D2-40 antibody One of our residents read about this antibody against lymphatic endothelium and wondered if its known by another name. Any help out there? Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Tue May 9 10:57:55 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Tue May 9 10:58:54 2006 Subject: [Histonet] Thermo and Fisher Scientific to Merge References: <3F500F8B416C554EBB21FF16642F72E959CE7D@marxchg03.mar.med.navy.mil> Message-ID: Thermo-Shandon-Electron-Richard-Allen-Fisher-Scientific.... ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Deltour, Douglas D. (HM2) Sent: Tue 5/9/2006 11:25 AM To: 'David Haagsma'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Thermo and Fisher Scientific to Merge Thermo-Shandon-Electron-Fisher Scientific. Anyone else want to join in? :) -----Original Message----- From: David Haagsma [mailto:DavidH@marketlabinc.com] Sent: Tuesday, May 09, 2006 11:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thermo and Fisher Scientific to Merge Has anybody seen this yet?... >From Thermo's site... Learn about Thermo Electron and Fisher Scientific combining to create the world's leading provider of laboratory products and services in the life, laboratory and health sciences industry. When the merger is completed, Thermo Fisher Scientific will offer offer complementary technologies, life science consumables, software and services to provide more complete and integrated solutions with an industry-leading global sales and service organization of nearly 7,500 professionals worldwide. Dave Haagsma MT Research Market Manager MarketLab Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> Mercer.edu Tue May 9 11:02:56 2006 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Tue May 9 11:03:35 2006 Subject: [Histonet] Thermo and Fisher Scientific to Merge In-Reply-To: Message-ID: <01M27U7XONCQ8X4F4Q@Macon2.Mercer.edu> vendorworld.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonner, Janet Sent: Tuesday, May 09, 2006 10:58 AM To: Deltour, Douglas D. (HM2); David Haagsma; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Thermo and Fisher Scientific to Merge Thermo-Shandon-Electron-Richard-Allen-Fisher-Scientific.... ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Deltour, Douglas D. (HM2) Sent: Tue 5/9/2006 11:25 AM To: 'David Haagsma'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Thermo and Fisher Scientific to Merge Thermo-Shandon-Electron-Fisher Scientific. Anyone else want to join in? :) -----Original Message----- From: David Haagsma [mailto:DavidH@marketlabinc.com] Sent: Tuesday, May 09, 2006 11:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thermo and Fisher Scientific to Merge Has anybody seen this yet?... >From Thermo's site... Learn about Thermo Electron and Fisher Scientific combining to create the world's leading provider of laboratory products and services in the life, laboratory and health sciences industry. When the merger is completed, Thermo Fisher Scientific will offer offer complementary technologies, life science consumables, software and services to provide more complete and integrated solutions with an industry-leading global sales and service organization of nearly 7,500 professionals worldwide. Dave Haagsma MT Research Market Manager MarketLab Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpmorken <@t> labvision.com Tue May 9 11:19:30 2006 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Tue May 9 11:19:46 2006 Subject: [Histonet] Thermo and Fisher Scientific to Merge Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA20328D8ED@usca0082k08.labvision.apogent.com> Hey, don't forget Lab Vision in there! Yes, it's true as of late 2004. Tim Morken Lab Vision - Neomarkers www.labvision.com Free webhosting for US State Histotechnology Societies: http://www.labvisioncorp.com/demowebsite/index.cfm -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Deltour, Douglas D. (HM2) Sent: Tuesday, May 09, 2006 8:25 AM To: 'David Haagsma'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Thermo and Fisher Scientific to Merge Thermo-Shandon-Electron-Fisher Scientific. Anyone else want to join in? :) -----Original Message----- From: David Haagsma [mailto:DavidH@marketlabinc.com] Sent: Tuesday, May 09, 2006 11:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thermo and Fisher Scientific to Merge Has anybody seen this yet?... >From Thermo's site... Learn about Thermo Electron and Fisher Scientific combining to create the world's leading provider of laboratory products and services in the life, laboratory and health sciences industry. When the merger is completed,?Thermo Fisher Scientific will offer offer complementary technologies, life science consumables, software and services to provide more complete and integrated solutions with an industry-leading global sales and service organization of nearly 7,500 professionals worldwide. Dave Haagsma MT Research Market Manager MarketLab Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jwatson <@t> gnf.org Tue May 9 11:30:17 2006 From: jwatson <@t> gnf.org (James Watson) Date: Tue May 9 11:30:25 2006 Subject: [Histonet] Thermo and Fisher Scientific to Merge Message-ID: Check out this web site about thermo buying fisher. http://www.nytimes.com/2006/05/09/business/09thermos.html?ex=1233547200&en=3139cf7b53cbe5d7&ei=5035&partner=MARKETWATCH -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Haagsma Sent: Tuesday, May 09, 2006 8:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thermo and Fisher Scientific to Merge Has anybody seen this yet?... >From Thermo's site... Learn about Thermo Electron and Fisher Scientific combining to create the world's leading provider of laboratory products and services in the life, laboratory and health sciences industry. When the merger is completed,?Thermo Fisher Scientific will offer offer complementary technologies, life science consumables, software and services to provide more complete and integrated solutions with an industry-leading global sales and service organization of nearly 7,500 professionals worldwide. Dave Haagsma MT Research Market Manager MarketLab Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lchausse <@t> nmh.org Tue May 9 11:35:48 2006 From: Lchausse <@t> nmh.org (Chaussey, Leslie) Date: Tue May 9 11:36:14 2006 Subject: [Histonet] Thermo and Fisher Scientific to Merge Message-ID: I heard a news report of this merger on the radio two mornings ago in the Chicago area but it didn't go into any detail. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of David Haagsma Sent: Tuesday, May 09, 2006 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thermo and Fisher Scientific to Merge Has anybody seen this yet?... >From Thermo's site... Learn about Thermo Electron and Fisher Scientific combining to create the world's leading provider of laboratory products and services in the life, laboratory and health sciences industry. When the merger is completed,?Thermo Fisher Scientific will offer offer complementary technologies, life science consumables, software and services to provide more complete and integrated solutions with an industry-leading global sales and service organization of nearly 7,500 professionals worldwide. Dave Haagsma MT Research Market Manager MarketLab Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- This message and any included attachments are intended only for the addressee. The information contained in this message is confidential and may constitute proprietary or non-public information under international, federal, or state laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail. From bliven.laura <@t> marshfieldclinic.org Tue May 9 12:16:37 2006 From: bliven.laura <@t> marshfieldclinic.org (bliven.laura@marshfieldclinic.org) Date: Tue May 9 12:16:19 2006 Subject: [Histonet] D2-40 Message-ID: <624d01c6738c$541c3bf0$6b05010a@mfldclinframe.org> D2-40 can be purchased from Dako (Cat.#M3619, IVD label) and it also might be found as Podoplanin. Modern Pathology (2006) 19, 708-716. Laura Bliven Histology Lab/IHC Marshfield Laboratories 1000 N. Oak Ave. Marshfield, WI 54449 From sbreeden <@t> nmda.nmsu.edu Tue May 9 12:46:24 2006 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue May 9 12:46:29 2006 Subject: [Histonet] Thermo-Fisher-Super-Mega-Corp Message-ID: Don't I remember reading/hearing something years ago about breaking up AT&T because they were in control of too much? What ever happened to anti-trust laws? Probably the same thing that happened to that non-drippy-spout-on-labware company, huh? Only thing it does is keep the business card printers and stationers in business!! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services POBox 4700 Albuquerque, NM 87196 (505)841-2576 (505)841-2518 FAX From thomas.crowell <@t> biogenidec.com Tue May 9 13:22:28 2006 From: thomas.crowell <@t> biogenidec.com (Thomas Crowell) Date: Tue May 9 13:22:36 2006 Subject: [Histonet] TMA consult In-Reply-To: Message-ID: Hello Histonetters, I'm looking for a person in the Boston/Cambridge area who would be intersested in providing consultation and training on the use of a Beecher Micro-Array instrument that we have here in our lab. Our TMA expert has left us and would like to maintain this function internally. I've budgeted approximately three days for this training. If anyone is interested, please contact me. Tom Crowell Biogen Idec Cambridge, MA 617-679-2634 From Rcartun <@t> harthosp.org Tue May 9 13:26:37 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue May 9 13:27:05 2006 Subject: [Histonet] D2-40 antibody In-Reply-To: References: Message-ID: <4460A69D02000009000AE429@hcnwgwds01.hh.chs> We are currently using Dako's D2-40 mAb. The antibody has been around several years (Kahn HJ, et al, Mod Pathol 2002;15:434-440), but has gained popularity more recently. Besides labeling lymphatic enodthelium, it will also label other types of cells and tumors (Kaposi's sarcoma, mesothelioma, seminoma, synovial sarcoma, and others). Richard >>> "Sebree Linda A." 05/09/06 9:13 AM >>> One of our residents read about this antibody against lymphatic endothelium and wondered if its known by another name. Any help out there? Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ChaseM <@t> childrensdayton.org Tue May 9 13:28:47 2006 From: ChaseM <@t> childrensdayton.org (Matthew Chase) Date: Tue May 9 13:29:48 2006 Subject: [Histonet] Ohio Histology Society Symposium Message-ID: May 12-13 is the Histology Society of Ohio Symposium/Convention. If you've come all this way, come about 10 minutes down the road to the Dayton Childrens Medical Center. We are hiring, we need a Histologist to fill a full time position. So come on down for an informal visit or go to the website at http://www.cmc-dayton.org and fill out an application. If you need directions or more information call us at 937-641-3358. We process around 5K cases per year. We do some immuno's with the Ventana ES Autostainer, have the PowerPath Information System, have a part time histologist, a full time histologist (that would be you) and myself, the super. Matt From Janet.Bonner <@t> FLHosp.org Tue May 9 13:39:25 2006 From: Janet.Bonner <@t> FLHosp.org (Bonner, Janet) Date: Tue May 9 13:41:33 2006 Subject: [Histonet] Thermo-Fisher-Super-Mega-Corp References: Message-ID: Have you seen what AT&T is doing now?! Cingular + ATT + SBC = THE NEW ATT Company!!!!! ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Breeden, Sara Sent: Tue 5/9/2006 1:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thermo-Fisher-Super-Mega-Corp Don't I remember reading/hearing something years ago about breaking up AT&T because they were in control of too much? What ever happened to anti-trust laws? Probably the same thing that happened to that non-drippy-spout-on-labware company, huh? Only thing it does is keep the business card printers and stationers in business!! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services POBox 4700 Albuquerque, NM 87196 (505)841-2576 (505)841-2518 FAX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Tue May 9 14:02:13 2006 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue May 9 14:00:00 2006 Subject: [Histonet] Ohio Histology Society Symposium Message-ID: <6CBA6DC98A079D408C87250591D9DFB802360DA6@bruexchange.digestivespecialists.com> I'm the tech that you would be replacing and I have to add my .02 to Matt's post. Children's is really a super place to work. They are a great bunch of people, and you never have to cut uterus or prostate. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Matthew Chase Sent: Tuesday, May 09, 2006 2:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ohio Histology Society Symposium May 12-13 is the Histology Society of Ohio Symposium/Convention. If you've come all this way, come about 10 minutes down the road to the Dayton Childrens Medical Center. We are hiring, we need a Histologist to fill a full time position. So come on down for an informal visit or go to the website at http://www.cmc-dayton.org and fill out an application. If you need directions or more information call us at 937-641-3358. We process around 5K cases per year. We do some immuno's with the Ventana ES Autostainer, have the PowerPath Information System, have a part time histologist, a full time histologist (that would be you) and myself, the super. Matt _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Robert.Lott <@t> bhsala.com Tue May 9 14:21:29 2006 From: Robert.Lott <@t> bhsala.com (Lott, Robert) Date: Tue May 9 14:21:49 2006 Subject: [Histonet] D2-40 antibody Message-ID: <65A043FA922929459928822312D887BADCA17C@bhsmailm3.bhsala.com> Several names for D2-40... one of the most common is podoplanin. Another is M2A Antigen. The original claim was that it was specific for lymphatic endothelium. It really does a nice job of staining it and doesn't stain vascular endothelium... useful for the diagnosis of lymphangiomas, lymphangiosarcomas, and determination of lymphatic invasion by neoplasm, but like a lot of antibodies it stains other things as well. For instance, turns out that it is an "excellent" marker for mesotheliomas, especially the epithelioid type. Follow this link to read an excellent the article about D2-40: http://www.propathlab.com/pdf/2005-10_D2-40.pdf Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology LabFirst / Trinity Medical Center 800 Montclair Road Birmingham, AL 35213 205-592-5388 phone 205-592-5646 fax robert.lott@bhsala.com ------------------------------------------------------------------------ ------------------------------------- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A. Sent: Tuesday, May 09, 2006 8:13 AM To: Histonet Subject: [Histonet] D2-40 antibody One of our residents read about this antibody against lymphatic endothelium and wondered if its known by another name. Any help out there? Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 ----------------------------------------- Confidentiality Notice: The information contained in this email message is privileged and confidential information and intended only for the use of the individual or entity named in the address. If you are not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this information is strictly prohibited. If you received this information in error, please notify the sender and delete this information from your computer and retain no copies of any of this information. From tissuearray <@t> hotmail.com Tue May 9 14:50:59 2006 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Tue May 9 14:51:07 2006 Subject: FW: [Histonet] TMA consult Message-ID: There is an array training website that answers a lot of questions. And has some instructional videos avalible. www.arrayworkshop.com >From: Thomas Crowell >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] TMA consult >Date: Tue, 9 May 2006 14:22:28 -0400 >MIME-Version: 1.0 >Received: from swlx162.swmed.edu ([199.165.152.162]) by >bay0-mc5-f13.bay0.hotmail.com with Microsoft SMTPSVC(6.0.3790.1830); Tue, 9 >May 2006 11:23:07 -0700 >Received: from localhost ([127.0.0.1] helo=swlx162.swmed.edu)by >swlx162.swmed.edu with esmtp (Exim 4.34)id 1FdWr0-0004gC-Pa; Tue, 09 May >2006 13:22:38 -0500 >Received: from [199.165.152.166] (helo=swlx166.swmed.edu)by >swlx162.swmed.edu with esmtp (Exim 4.34) id 1FdWqw-0004g5-0hfor >histonet@lists.utsouthwestern.edu; Tue, 09 May 2006 13:22:35 -0500 >Received: from ip18.biogen.com >([198.180.131.18]helo=camsmtphost01.biogenidec.com)by swlx166.swmed.edu >with esmtp (Exim 4.44) id 1FdWqu-00033C-RDfor >histonet@lists.utsouthwestern.edu; Tue, 09 May 2006 13:22:33 -0500 >X-Message-Info: LsUYwwHHNt2FZ/IbjHvlecp0iWlDoeqAJZ0GmPE3XM4= >X-Mailer: Lotus Notes Release 6.5.4 March 27, 2005 >X-Scan-Signature: 690e9200c080ca55fd7a8d0fb0193eeb >X-Content-Filtered-By: Mailman/MimeDel 2.1.5 >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.1.5 >Precedence: list >List-Id: For the exchange of information pertaining to histotechnology >andrelated fields >List-Unsubscribe: >, > >List-Archive: >List-Post: >List-Help: >List-Subscribe: >, >Errors-To: histonet-bounces@lists.utsouthwestern.edu >X-Scan-Signature: 1d06f19e0b5ef274560ff195311cd0ef >X-SA-Exim-Connect-IP: 127.0.0.1 >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >X-Spam-Checker-Version: SpamAssassin 2.64 (2004-01-11) on swlx162.swmed.edu >X-Spam-Level: X-Spam-Status: No, hits=0.0 required=5.5 tests=none >autolearn=no version=2.64 >X-SA-Exim-Version: 4.0 (built Wed, 07 Apr 2004 12:14:41 -0500) >X-SA-Exim-Scanned: Yes (on swlx162.swmed.edu) >Return-Path: histonet-bounces@lists.utsouthwestern.edu >X-OriginalArrivalTime: 09 May 2006 18:23:08.0931 (UTC) >FILETIME=[9F769D30:01C67395] > >Hello Histonetters, > >I'm looking for a person in the Boston/Cambridge area who would be >intersested in providing consultation and training on the use of a >Beecher Micro-Array instrument that we have here in our lab. Our TMA >expert has left us and would like to maintain this function internally. >I've budgeted approximately three days for this training. If anyone is >interested, please contact me. > >Tom Crowell >Biogen Idec >Cambridge, MA >617-679-2634 >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mverdu <@t> histopat.es Wed May 10 01:05:04 2006 From: mverdu <@t> histopat.es (Montse verdu) Date: Wed May 10 01:05:29 2006 Subject: [Histonet] Anti-Human Hepatocite Message-ID: <000e01c673f7$b378e9c0$3800a8c0@Histopat.com> Dear histonetters, Anybody knows if the clone OCH1E5 of anti-Human Hepatocyte is another name for the clone HEPPAR-1? I read this information in an article and I am surprise. Can anybody say me if it is true? Thanks for your help. Montse Verd? histopat LABORATORIS Tel. 932033000 Fax 932802160 mverdu@histopat.es From jfern <@t> unimelb.edu.au Wed May 10 01:10:10 2006 From: jfern <@t> unimelb.edu.au (John Fernandez) Date: Wed May 10 01:10:18 2006 Subject: [Histonet] Re: Golgi stain question/Rapid Golgi Stain (FD Neurotech) Message-ID: <1706.128.250.186.193.1147241410.squirrel@webmail.unimelb.edu.au> Hi all, our lab has recently purchased and used the Rapid Golgi Stain Kit from FD Neurotech, resulting in very impressive staining down to dendritic spine level, however as Julie Heinrich found, within 24 hours this staining becomes very granular with loss of distinct morphology that was otherwise seen previously. It progressively worsens over time. Has anyone successfully used the FD Neurotech Rapid Golgi kit? Or does anyone know of a mounting medium that can be used to adequately stop the reaction that is taking place? Any ideas on what could be causing this progressive deterioration of staining would be very much appreciated. Thanks in advance, John John Fernandez Research Assistant Department of Medicine University of Melbourne Austin & Repatriation Medical Centre Heidelberg, VIC 3084 Ph: (03) 9496 3257 Re: Golgi stain question From: "J. A. Kiernan" -------------------------------------------------------------------------------- On Fri, 30 Mar 2001, Julie Heinrich wrote: > I am terribly sorry to bother you- I have been looking through the > histonet web page and have found several helpful replies from you > about the Gibb & Kolb Golgi stain method. I haven't managed to > figure out how to properly post a question there - You can't. It's a collection of old (archived) communications. To ask or answer questions you have to subscribe to the listserver. This is done by sending an email to histonet@pathology.swmed.edu with the one word subscribe in the Subject line. Nothing else. You'll then get an automatic reply from the listserver telling you all about it. > I'm attempting to use the method on avian tissue. I have obtained > some decent looking tissue so far (though the stain is a dark > tan/brown, rather than the preferable black that I had expected) yet > after time the stain turns very 'grainy'. Within a matter of just 24 > hours, the dendrites/spines look like collections of dots rather than > complete structures, and it gets worse with time. > Do you have any idea why this might be happening? (I'm following > their protocol to the best of my knowledge, and use fresh solutions > each time I run tissue) A graduate student here called Tim Ho did great numbers of Kolb Golgis on rat brains a few years ago. He went to Kolb's lab in Lethbridge, Alta for guidance. His initial problem was that the unstained spaces between the black cells were pale green and a bit granular. If I remember rightly, the washing after the Golgi-Cox solution needed to be more thorough. Your problem is different, and may relate to the mounting medium. Traditionally the sections were mounted in thick Canada balsam without coverslips. A modern synthetic medium + coverslip can be followed by fading, but I haven't heard of this happening in 24 hours. 6 months, yes. Are you cutting vibratome sections of adequate thickness? I think you must be doing something wrong at or after the sectioning step, but don't know what. Sorry I can't be more helpful. ---------------------------------------- John A. Kiernan Department of Anatomy & Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan@uwo.ca http://publish.uwo.ca/~jkiernan -------------------------------------------------------------------------------- << Previous Message | Next Message >> -- From jnocito <@t> satx.rr.com Wed May 10 04:57:46 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed May 10 04:57:57 2006 Subject: [Histonet] Thermo-Fisher-Super-Mega-Corp References: Message-ID: <002901c67418$30c14f10$1bb30b43@yourxhtr8hvc4p> looks we have one-stop shopping, just like Wal-Mart & Target stores, at least there, there is some sort of competition. Never did like a monopoly, even as a game. Hmmmmmm, so much for that personal touch. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Bonner, Janet" To: "Breeden, Sara" ; Sent: Tuesday, May 09, 2006 1:39 PM Subject: RE: [Histonet] Thermo-Fisher-Super-Mega-Corp Have you seen what AT&T is doing now?! Cingular + ATT + SBC = THE NEW ATT Company!!!!! ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Breeden, Sara Sent: Tue 5/9/2006 1:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thermo-Fisher-Super-Mega-Corp Don't I remember reading/hearing something years ago about breaking up AT&T because they were in control of too much? What ever happened to anti-trust laws? Probably the same thing that happened to that non-drippy-spout-on-labware company, huh? Only thing it does is keep the business card printers and stationers in business!! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services POBox 4700 Albuquerque, NM 87196 (505)841-2576 (505)841-2518 FAX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.392 / Virus Database: 268.5.5/335 - Release Date: 5/9/2006 From funderwood <@t> mcohio.org Wed May 10 07:47:15 2006 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Wed May 10 07:47:46 2006 Subject: [Histonet] Thermo and Fisher Scientific to Merge Message-ID: I think they are going to merge with the National Hockey League as well. >>> "Morken, Tim - Labvision" 05/09/06 12:19PM >>> Hey, don't forget Lab Vision in there! Yes, it's true as of late 2004. Tim Morken Lab Vision - Neomarkers www.labvision.com Free webhosting for US State Histotechnology Societies: http://www.labvisioncorp.com/demowebsite/index.cfm -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Deltour, Douglas D. (HM2) Sent: Tuesday, May 09, 2006 8:25 AM To: 'David Haagsma'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Thermo and Fisher Scientific to Merge Thermo-Shandon-Electron-Fisher Scientific. Anyone else want to join in? :) -----Original Message----- From: David Haagsma [mailto:DavidH@marketlabinc.com] Sent: Tuesday, May 09, 2006 11:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Thermo and Fisher Scientific to Merge Has anybody seen this yet?... >From Thermo's site... Learn about Thermo Electron and Fisher Scientific combining to create the world's leading provider of laboratory products and services in the life, laboratory and health sciences industry. When the merger is completed, Thermo Fisher Scientific will offer offer complementary technologies, life science consumables, software and services to provide more complete and integrated solutions with an industry-leading global sales and service organization of nearly 7,500 professionals worldwide. Dave Haagsma MT Research Market Manager MarketLab Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pathrm35 <@t> adelphia.net Wed May 10 09:17:05 2006 From: pathrm35 <@t> adelphia.net (pathrm35@adelphia.net) Date: Wed May 10 09:17:17 2006 Subject: [Histonet] dictation systems for grossing tissue Message-ID: <17095950.1147270625790.JavaMail.root@web11> What dictation systems are you all using for your tissue grossing? I'd like to hear the pros and cons on as many systems as possible. Also, pricing if possible. Thanks in advance, Ron Martin From immunoqueen <@t> yahoo.com Wed May 10 10:01:34 2006 From: immunoqueen <@t> yahoo.com (Jennifer Leigh) Date: Wed May 10 10:01:44 2006 Subject: [Histonet] Processor Preferences?? Message-ID: <20060510150134.84781.qmail@web51312.mail.yahoo.com> Dear Histonet, We are in the process of buying a new tissue processor and are currently considering the Shandon Excelsior, the Shandon Pathcentre, and the Leica ASP300. I would welcome input and personal preferences from Histonetters who use these instruments. Thank you in advance! Jennifer Oskins Asst. Senior Biologist Eli Lilly and Company Jennifer L. Oskins immunoqueen@yahoo.com --------------------------------- Yahoo! Messenger with Voice. PC-to-Phone calls for ridiculously low rates. From gliuygao <@t> hotmail.com Wed May 10 10:45:38 2006 From: gliuygao <@t> hotmail.com (yan gao) Date: Wed May 10 10:45:49 2006 Subject: [Histonet] IHC on Cheek cells Message-ID: Hi, Histonet. I am staining phosoph-MEK on cheek cells. Here is the way how I collected cheek cells. I used cotton tips scrape inside of human mouth. then I soak the tip into the fixative offered by Thermo shandon for cytospin. Then I cytospined cells to slides. While I stained pMEK, I brought a rabbit IgG with it for negative control. I got a positive stain on cheek cells. Cells were very sticky. Anyone has IHC experience on buccal cells would like share with me, I really appreciated. Thanks a lot. Yan Gao Novartis From juan.gutierrez <@t> christushealth.org Wed May 10 10:49:23 2006 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Wed May 10 10:49:35 2006 Subject: [Histonet] Processor Preferences?? Message-ID: We've had the Leica ASP 300 for about four years now. We've had to replace the pump twice, the fuse-power cord assembly had to be replaced also. Every time we've had to order a part from Leica it's been very frustrating. The wrong part has been sent more often than the correct one and that is after we've had to contact Germany to get a fax of what the part looks like. We still got the wrong part. This year our processor has worked "properly" for a grand total of THREE weeks, and not in a row. Thank God we have an old but reliable VIP as a back up. All of this is well documented in our Biomed department so no threatening calls please. Look at the disclaimer below. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Leigh Sent: Wednesday, May 10, 2006 10:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processor Preferences?? Dear Histonet, We are in the process of buying a new tissue processor and are currently considering the Shandon Excelsior, the Shandon Pathcentre, and the Leica ASP300. I would welcome input and personal preferences from Histonetters who use these instruments. Thank you in advance! Jennifer Oskins Asst. Senior Biologist Eli Lilly and Company Jennifer L. Oskins immunoqueen@yahoo.com --------------------------------- Yahoo! Messenger with Voice. PC-to-Phone calls for ridiculously low rates. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SAllen <@t> exchange.hsc.mb.ca Wed May 10 11:03:28 2006 From: SAllen <@t> exchange.hsc.mb.ca (Sharon Allen) Date: Wed May 10 11:05:04 2006 Subject: [Histonet] Xylol substitutes Message-ID: <36FE435D6D2F1D489174B22362A961B66B8335@hscxntmx0006.hsc.mb.ca> Does anyone use xylol substitutes, if so how good are they? We do CJD testing & "the powers that be" don't like to pay for getting rid of contaminated xylol. I don't want to have to start testing different substitutes, so any help would be greatly appreciated. Thanks Sharon sallen@hsc.mb.ca This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. From HornHV <@t> archildrens.org Wed May 10 11:13:11 2006 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed May 10 11:13:55 2006 Subject: [Histonet] Processor Preferences?? Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C63038BE06D@EMAIL.archildrens.org> We have a ASP 300 too. The first one we received was a 'lemon" and Leica replaced it. The replacement has run very well. No downtime at all. It has been very reliable for us. Leica was easy to work with when our first machine came and we had so many problems. They switched out processors for us and this one is a dream. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of GUTIERREZ, JUAN Sent: Wednesday, May 10, 2006 10:49 AM To: Jennifer Leigh; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Processor Preferences?? We've had the Leica ASP 300 for about four years now. We've had to replace the pump twice, the fuse-power cord assembly had to be replaced also. Every time we've had to order a part from Leica it's been very frustrating. The wrong part has been sent more often than the correct one and that is after we've had to contact Germany to get a fax of what the part looks like. We still got the wrong part. This year our processor has worked "properly" for a grand total of THREE weeks, and not in a row. Thank God we have an old but reliable VIP as a back up. All of this is well documented in our Biomed department so no threatening calls please. Look at the disclaimer below. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Leigh Sent: Wednesday, May 10, 2006 10:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processor Preferences?? Dear Histonet, We are in the process of buying a new tissue processor and are currently considering the Shandon Excelsior, the Shandon Pathcentre, and the Leica ASP300. I would welcome input and personal preferences from Histonetters who use these instruments. Thank you in advance! Jennifer Oskins Asst. Senior Biologist Eli Lilly and Company Jennifer L. Oskins immunoqueen@yahoo.com --------------------------------- Yahoo! Messenger with Voice. PC-to-Phone calls for ridiculously low rates. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From thoward <@t> unm.edu Wed May 10 11:22:11 2006 From: thoward <@t> unm.edu (Tamara Howard) Date: Wed May 10 11:22:18 2006 Subject: [Histonet] RE:contract lab query Message-ID: Thanks to everyone who responded to my request for info on contract labs - we had no idea there were so many! I've forwarded the replies on the the person who asked me for the information; I was told this morning that everything had been forwarded on to the PI who made the original request. Thanks also to those of you who sent recommendations - those were very helpful (and all for the same place, I should add!). I passed those along, too. Tamara |--------------------------------------------------| Tamara Howard Department of Cell Biology and Physiology University of New Mexico - Health Sciences Center Albuquerque, NM 87131 thoward@unm.edu |--------------------------------------------------| From gentras <@t> vetmed.auburn.edu Wed May 10 11:28:24 2006 From: gentras <@t> vetmed.auburn.edu (Atoska S. Gentry) Date: Wed May 10 11:28:38 2006 Subject: Fwd: [Histonet] Xylol substitutes Message-ID: <7.0.1.0.0.20060510112551.01938e98@vetmed.auburn.edu> Hello, both Hem-o-de and/or Mercedes Medical's Z-sub work fine for me, but I still use a final stain dish of xylene to coverslip from. Best wishes, Atoska >Date: Wed, 10 May 2006 11:03:28 -0500 >From: Sharon Allen >To: "Histonet (E-mail)" >X-Mailer: Internet Mail Service (5.5.2653.19) >X-Scan-Signature: fa1887bb5270b51defc3a2885a1d8a92 >Cc: >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.1.5 >List-Id: For the exchange of information pertaining to histotechnology and > related fields >List-Unsubscribe: >, > > >List-Archive: >List-Post: >List-Help: >List-Subscribe: , > >Sender: histonet-bounces@lists.utsouthwestern.edu >X-Scan-Signature: 9efafbcd85335e515bdf0d9adb1caaeb >X-SA-Exim-Connect-IP: 127.0.0.1 >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >Subject: [Histonet] Xylol substitutes >X-Spam-Checker-Version: SpamAssassin 2.64 (2004-01-11) on swlx162.swmed.edu >X-Spam-Level: >X-Spam-Status: No, hits=0.0 required=5.5 tests=none autolearn=no version=2.64 >X-SA-Exim-Version: 4.0 (built Wed, 07 Apr 2004 12:14:41 -0500) >X-SA-Exim-Scanned: Yes (on swlx162.swmed.edu) >X-spam: ESP<-125>=RBL:<-140> SHA:<15> UHA:<0> SLS:<0> BAYES:<0> SenderID:<0> > Spam Dictionary (TRU10):<0> Obscenities Dictionary > (TRU10):<0> Scam Dictionary (TRU10):<0> Adult Dictionary > (TRU10):<0> Embed HTML Dictionary (TRU10):<0> Float > Dictionary (TRU10):<0> HTML Dictionary (TRU10):<0> URL > Real-Time Signatures:<0> Spam Dictionary 2 (TRU10):<0> > SIG: AAAAAAAAAAAAAAAABgdkkzcJYswTzLiXAPvDqYCkysjBZcu0NejMpAPHwz8d > q37GXPIZ-5zU6kcdAU-MTNLw6Qr7zhqIdxwIE_B9r3OB3pKvod8Jk-n62hZy > SoOssncAv7Xdw1uaqiNAAAAAAAAh505lYmw7IZSrFV0v2FrIe> > > >Does anyone use xylol substitutes, if so how good are they? >We do CJD testing & "the powers that be" don't like to pay for getting rid >of contaminated xylol. I don't want to have to start testing different >substitutes, so any help would be greatly appreciated. >Thanks >Sharon >sallen@hsc.mb.ca > >This e-mail and/or any documents in this transmission is intended >for the address(s) only and may contain legally privileged or >confidential information. Any unauthorized use, disclosure, >distribution, copying or dissemination is strictly prohibited. If >you receive this transmission in error, please notify the sender >immediately and return the original. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Wed May 10 11:35:35 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed May 10 11:35:44 2006 Subject: [Histonet] Xylol substitutes Message-ID: <09C945920A6B654199F7A58A1D7D1FDE017176F6@lsexch.lsmaster.lifespan.org> There are many xylene substitutes in use today, but as far as I know (someone correct me if I'm wrong) they all have to be disposed of as hazardous waste. And many of them cost more to purchase than xylene. So, financial considerations are probably not a good reason to switch. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > Sharon Allen > Sent: Wednesday, May 10, 2006 9:03 AM > To: Histonet (E-mail) > Subject: [Histonet] Xylol substitutes > > > Does anyone use xylol substitutes, if so how good are they? > We do CJD testing & "the powers that be" don't like to pay for getting rid > of contaminated xylol. I don't want to have to start testing different > substitutes, so any help would be greatly appreciated. > Thanks > Sharon > sallen@hsc.mb.ca > > This e-mail and/or any documents in this transmission is intended for the > address(s) only and may contain legally privileged or confidential > information. Any unauthorized use, disclosure, distribution, copying or > dissemination is strictly prohibited. If you receive this transmission in > error, please notify the sender immediately and return the original. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From funderwood <@t> mcohio.org Wed May 10 11:38:10 2006 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Wed May 10 11:38:21 2006 Subject: [Histonet] Processor Preferences?? Message-ID: I can't comment on those. But I have had a VIP 5 for about a year now and it is working grea,t so far(insert sound of knocking on wood here). Fred >>> "Jennifer Leigh" 05/10/06 11:01AM >>> Dear Histonet, We are in the process of buying a new tissue processor and are currently considering the Shandon Excelsior, the Shandon Pathcentre, and the Leica ASP300. I would welcome input and personal preferences from Histonetters who use these instruments. Thank you in advance! Jennifer Oskins Asst. Senior Biologist Eli Lilly and Company Jennifer L. Oskins immunoqueen@yahoo.com --------------------------------- Yahoo! Messenger with Voice. PC-to-Phone calls for ridiculously low rates. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Wed May 10 11:45:39 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed May 10 11:45:51 2006 Subject: [Histonet] IHC on Cheek cells In-Reply-To: References: Message-ID: <6.0.0.22.1.20060510104228.01b65490@gemini.msu.montana.edu> We used wooden tongue depressors to scrape cheeks for buccal smears - that way you do not have cells trapped in cotton fibers. There are scrapers, plastic that may work better, you can still resuspend the cells off the ends - agitate the tubes containing scraper with a vortex mixer to let cells go into PBS. I think you could cytospin the cells onto slides easily. At 09:45 AM 5/10/2006, you wrote: > Hi, Histonet. > > I am staining phosoph-MEK on cheek cells. Here is the way how I > collected cheek cells. I used cotton tips scrape inside of human > mouth. then I soak the tip into the fixative offered by Thermo > shandon for cytospin. Then I cytospined cells to slides. > While I stained pMEK, I brought a rabbit IgG with it for negative > control. I got a positive stain on cheek cells. Cells were very > sticky. > Anyone has IHC experience on buccal cells would like share with me, I > really appreciated. Thanks a lot. > > Yan Gao > > Novartis >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From llewllew <@t> shaw.ca Wed May 10 11:51:58 2006 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Wed May 10 11:52:10 2006 Subject: [Histonet] Xylol substitutes References: <36FE435D6D2F1D489174B22362A961B66B8335@hscxntmx0006.hsc.mb.ca> Message-ID: <001601c67452$0d9bc350$130e4246@yourlk4rlmsu> If the xylene is considered to be contaminated, then would that not be the case for any other clearant used to replace it? Do these powers consider it to be more acceptable to pay for getting rid of contaminated limonene, for example, instead of contaminated xylene? Is the alcohol disposed of in a safe manner as well? Do they pay for this? This should be a bigger issue than just the cost, surely. Bryan Llewellyn ----- Original Message ----- From: "Sharon Allen" To: "Histonet (E-mail)" Sent: Wednesday, May 10, 2006 9:03 AM Subject: [Histonet] Xylol substitutes > > Does anyone use xylol substitutes, if so how good are they? > We do CJD testing & "the powers that be" don't like to pay for getting rid > of contaminated xylol. I don't want to have to start testing different > substitutes, so any help would be greatly appreciated. > Thanks > Sharon > sallen@hsc.mb.ca > > This e-mail and/or any documents in this transmission is intended for the > address(s) only and may contain legally privileged or confidential > information. Any unauthorized use, disclosure, distribution, copying or > dissemination is strictly prohibited. If you receive this transmission in > error, please notify the sender immediately and return the original. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Karen.Heckford <@t> CHW.edu Wed May 10 11:51:35 2006 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Wed May 10 11:53:16 2006 Subject: [Histonet] PowerPath Tamtron Message-ID: Hello Histonetters, Does anyone know of a way that I could look up past cases by keywords? For example looking up IHC by their name. I would like to be able to do this so I can find tissues that would make good controls. I have searched and searched and cannot figure out how to do this. Perhaps it does not exist. Thanks, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathololgy Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu From kjohnson <@t> covx.com Wed May 10 12:07:27 2006 From: kjohnson <@t> covx.com (Kimberly Johnson) Date: Wed May 10 12:07:37 2006 Subject: [Histonet] Slide Labeler Message-ID: <96DACB13ECACA645A47020B3E136776C0FA054@covxmail.covx.com> Hello Histonet. First let me say, long time reader, first time poster, so this is very exciting! My small biotech company has decided it is time for me to get a slide labeler! I would love some input as to what you all think works well, keep in mind this is a small company doing very simple and not much IHC. Thanks for your time! Kim From rjbuesa <@t> yahoo.com Wed May 10 12:07:55 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 10 12:08:02 2006 Subject: [Histonet] Xylol substitutes In-Reply-To: <36FE435D6D2F1D489174B22362A961B66B8335@hscxntmx0006.hsc.mb.ca> Message-ID: <20060510170755.2198.qmail@web61215.mail.yahoo.com> Sharon: I am sending privately a procedure I have that substitutes xylene with mineral oil. It is 100 times less toxic than xylene and 3 times cheaper. Ren? J. Sharon Allen wrote: Does anyone use xylol substitutes, if so how good are they? We do CJD testing & "the powers that be" don't like to pay for getting rid of contaminated xylol. I don't want to have to start testing different substitutes, so any help would be greatly appreciated. Thanks Sharon sallen@hsc.mb.ca This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1¢/min. From Nancy.Temple <@t> ssfhs.org Wed May 10 12:23:11 2006 From: Nancy.Temple <@t> ssfhs.org (Temple Nancy) Date: Wed May 10 12:23:25 2006 Subject: [Histonet] Processor Preferences?? Message-ID: We have 2 VIP tissue processors, a VIP 3000 that is almost 18 years old and still processing daily, and a VIP 5 that is 5 years old and also processes daily. Have had minimal problems. We have been very pleased with both. Nancy Temple, HT(ASCP) Histology Supervisor St. Francis Hospital & Health Centers 8111 S. Emerson Ave. Indianapolis, In 46237 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Leigh Sent: Wednesday, May 10, 2006 11:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processor Preferences?? Dear Histonet, We are in the process of buying a new tissue processor and are currently considering the Shandon Excelsior, the Shandon Pathcentre, and the Leica ASP300. I would welcome input and personal preferences from Histonetters who use these instruments. Thank you in advance! Jennifer Oskins Asst. Senior Biologist Eli Lilly and Company Jennifer L. Oskins immunoqueen@yahoo.com --------------------------------- Yahoo! Messenger with Voice. PC-to-Phone calls for ridiculously low rates. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From 1dpeterson <@t> meriter.com Wed May 10 12:27:17 2006 From: 1dpeterson <@t> meriter.com (Peterson, Dan) Date: Wed May 10 12:27:25 2006 Subject: [Histonet] Dictation System Message-ID: <328CBAE62F31C642B422970E879DFADC01A69508@pcwex01> Ron, If Lanier comes around, RUN!!!! We had Dictaphone and they were good (back when we had tapes) We then were looking for digital and Lanier (who our hospital used, emphasize "used") said we could go digital with their equipment and piggyback with the hospital's server to save money. Needless to say, we couldn't use the hospitals server, the Pathologists didn't like the handheld recorders (and they still don't like the new Prot?g?s) and the hospital switched to Dictaphone! $18000 later we're got Pathologists hand writing their diagnosis, and PA's logging on the equipment as guests. Dictaphone (as I have seen in other labs) seems to be the way to go. Daniel R Peterson HT(ASCP) Histopathology Section Head General Medical Laboratories (608)-267-6557 1dpeterson@meriter.com CONFIDENTIALITY NOTICE: This message (including any attachments) is intended for the sole use of the individual and entity to whom it is addressed. This message may contain information that is confidential and is protected by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply email and then delete the message. Thank you. From Dorothy.L.Webb <@t> HealthPartners.Com Wed May 10 12:30:34 2006 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Wed May 10 12:30:42 2006 Subject: [Histonet] decal Message-ID: <0E394B648E5284478A6CCB78E5AFDA270171FDAA@hpes1.HealthPartners.int> Does anyone use the microwave processor for decalcification of specimens? If so, I would appreciate any protocols in this area. We have the Thermo Tissue-Wave which we use daily for processing small biopsies and cell blocks. Thanks ahead of time for any and all information either through my direct Email or this venue!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From drgrant <@t> cmh.edu Wed May 10 12:35:56 2006 From: drgrant <@t> cmh.edu (Grant, Debra, R) Date: Wed May 10 12:36:03 2006 Subject: [Histonet] (no subject) Message-ID: <6F434E27D5DE944B9E898984CADDD149043A67@exchmail.CMH.Internal> Hi All, Are there any labs running side by side slides on old lot and new lot antibodies every time you get a new lot of antibody? Please be very specific on your answers. Thanks! Debby R. Grant HT (ASCP) QIHC Histology Coordinator The Children's Mercy Hospital 2401 Gillham Road KC, Mo 64108 Lab(816)234-3827 Fax(816)802-1492 From dsantana <@t> pmaonline.com Wed May 10 12:02:34 2006 From: dsantana <@t> pmaonline.com (Santana, Diane) Date: Wed May 10 12:40:13 2006 Subject: [Histonet] tissue processor Message-ID: <4C96AA62BADFD81180CB0002A5AD67FB071139C8@MAILPMA> Jennifer, I have used the Lecia at another faculty, and it had some problems, but I had always preferred the Sakura VIP until now. I got a lemon and the service dept is horrible, in fact we are trying to get them to replace it. Actually the service has been so bad I would love to dump the whole company. Anyway, sorry for venting, but I would try the thermo at this time. Did you know that Richard Allan also has one? Good luck, Diane Santana PMA Haverhill, Mass. From ander093 <@t> tc.umn.edu Wed May 10 12:41:04 2006 From: ander093 <@t> tc.umn.edu (LuAnn Anderson) Date: Wed May 10 12:41:52 2006 Subject: [Histonet] Xylol substitutes In-Reply-To: <001601c67452$0d9bc350$130e4246@yourlk4rlmsu> References: <36FE435D6D2F1D489174B22362A961B66B8335@hscxntmx0006.hsc.mb.ca> <001601c67452$0d9bc350$130e4246@yourlk4rlmsu> Message-ID: <6.2.3.4.0.20060510123948.03b168b8@ander093.email.umn.edu> I couldn't agree more. We have a very strict policy for the disposal of CJD waste--liquid and solids alike. LuAnn Anderson Neuropathology Lab University of Minnesota At 11:51 AM 5/10/2006, Bryan Llewellyn wrote: >If the xylene is considered to be contaminated, then would that not >be the case for any other clearant used to replace it? Do these >powers consider it to be more acceptable to pay for getting rid of >contaminated limonene, for example, instead of contaminated >xylene? Is the alcohol disposed of in a safe manner as well? Do >they pay for this? This should be a bigger issue than just the cost, surely. > >Bryan Llewellyn > > > >----- Original Message ----- From: "Sharon Allen" >To: "Histonet (E-mail)" >Sent: Wednesday, May 10, 2006 9:03 AM >Subject: [Histonet] Xylol substitutes > > >> >>Does anyone use xylol substitutes, if so how good are they? >>We do CJD testing & "the powers that be" don't like to pay for getting rid >>of contaminated xylol. I don't want to have to start testing different >>substitutes, so any help would be greatly appreciated. >>Thanks >>Sharon >>sallen@hsc.mb.ca >> >>This e-mail and/or any documents in this transmission is intended >>for the address(s) only and may contain legally privileged or >>confidential information. Any unauthorized use, disclosure, >>distribution, copying or dissemination is strictly prohibited. If >>you receive this transmission in error, please notify the sender >>immediately and return the original. >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LuckG <@t> empirehealth.org Wed May 10 13:28:57 2006 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Wed May 10 13:29:03 2006 Subject: [Histonet] Ventana/RMC Tissue Processor Cassette Organizers Message-ID: <6BB8BC4519AAB844B174FC739A679BBC1C2FAB@IRMEXCH01.irm.inhs.org> Hello all, I am looking for any of the original tissue cassette organizers (120 capacity, square and wire like with individual cassette slots that hold them in a fixed vertical position). When Ventana announced they were stopping support for these instruments (i.e. the RMC MVP I & II, the P1530 and finally the Rennaisance) I tried to buy several replacement backups and was told that one individual had purchased their entire remaining inventory (approx 20+). I would greatly appreciate a response from anyone who has some left over that they are no longer using or from someone who has taken it upon themselves to have replacements manufactured by a secondary source. Thanks in advance, Greg Greg Luck Anatomic Path Spvr Deaconess Med Cntr 800 W. 5th Ave Spokane, WA 99204 Offc 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmc.org From Diane.Gladney <@t> se.amedd.army.mil Wed May 10 13:18:52 2006 From: Diane.Gladney <@t> se.amedd.army.mil (Gladney, Diane C Ms MACH) Date: Wed May 10 13:31:08 2006 Subject: [Histonet] Processor Preferences?? Message-ID: <4D55B2E997EFAE4DA6081DDE100B8302DE8FF5@amedmlsermc133> We purchased a Leica ASP 300 last June. It has been a great processor. The software is user friendly. I went to Chicago to the user training (included in purchase price) which was most helpful. We are buying a second Leica ASP 300 to replace our very, very old table top VIP (25+years old) which is our back-up processor. The service and technical help from the company is superior. I have had few problems with the instrument (clock error). Those problems were fixed almost immediately. I was assured that if they could not fix it to my satisfaction, then I would be given another new processor to replace it. I am most impressed with the processor, the service and the follow-up after a service call. These processors are assembled in Germany then shipped to the USA. Anything can happen in shipment. Thanks, Diane Diane C. Gladney, HT (ASCP) Supervisor, Histology Dept. Moncrief Army Community Hospital Dept. of Pathology 4500 Stuart St. FT. Jackson, SC 29207 Email: diane.gladney@se.amedd.army.mil Phone: 803-751-2530 FAX: 803-751-7829 DSN: 734-2530 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of GUTIERREZ, JUAN Sent: Wednesday, May 10, 2006 11:49 AM To: Jennifer Leigh; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Processor Preferences?? We've had the Leica ASP 300 for about four years now. We've had to replace the pump twice, the fuse-power cord assembly had to be replaced also. Every time we've had to order a part from Leica it's been very frustrating. The wrong part has been sent more often than the correct one and that is after we've had to contact Germany to get a fax of what the part looks like. We still got the wrong part. This year our processor has worked "properly" for a grand total of THREE weeks, and not in a row. Thank God we have an old but reliable VIP as a back up. All of this is well documented in our Biomed department so no threatening calls please. Look at the disclaimer below. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer Leigh Sent: Wednesday, May 10, 2006 10:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Processor Preferences?? Dear Histonet, We are in the process of buying a new tissue processor and are currently considering the Shandon Excelsior, the Shandon Pathcentre, and the Leica ASP300. I would welcome input and personal preferences from Histonetters who use these instruments. Thank you in advance! Jennifer Oskins Asst. Senior Biologist Eli Lilly and Company Jennifer L. Oskins immunoqueen@yahoo.com --------------------------------- Yahoo! Messenger with Voice. PC-to-Phone calls for ridiculously low rates. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LuckG <@t> empirehealth.org Wed May 10 13:52:14 2006 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Wed May 10 13:52:28 2006 Subject: [Histonet] tissue processor feedback Message-ID: <6BB8BC4519AAB844B174FC739A679BBC1C2FAC@IRMEXCH01.irm.inhs.org> Hello all, Can't help chiming in on tissue processors. I have been through two VIP's, have demoed others as well in the past and currently have two Ventanas. I had one of the first geeneration VIP's on the west coast back in 1980. This instrument today still has no equal in processing power. I purchased a VIP 3000 expecting similar capabilites and was sorely disappointed. They had removed a second pump (from the original model) that had provided the agitation and replaced it with "periodic tidal flow" to compensate. In short if you are confronted with processing large (and small) specimens there is no substitution for aggressive agitation whether it be in the form of continually moving the reagents around at a vigorous pace or physically moving the tissues around within the retort chamber. I'm not that familiar with what the current marketplace has to offer but when I go to purchase our next processor the form of agitation will be my starting criteria. Vacuum, pressure and tidal flow are not a substitute for good old vigorous physical agitation. As an analogy look at the difference in the speed of diffusion of reagents through a tissue section on a slide when you hand stain it as opposed to when it's subjected to the slow (or nonexistent) agitation that most automated stainers provide. That's all. Greg Luck Anatomic Path Spvr Deaconess Med Cntr 800 W. 5th Ave Spokane, WA 99204 Offc 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmedicalcenter.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Santana, Diane Sent: Wednesday, May 10, 2006 10:03 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] tissue processor Jennifer, I have used the Lecia at another faculty, and it had some problems, but I had always preferred the Sakura VIP until now. I got a lemon and the service dept is horrible, in fact we are trying to get them to replace it. Actually the service has been so bad I would love to dump the whole company. Anyway, sorry for venting, but I would try the thermo at this time. Did you know that Richard Allan also has one? Good luck, Diane Santana PMA Haverhill, Mass. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgirlnow <@t> msn.com Wed May 10 14:04:24 2006 From: godsgirlnow <@t> msn.com (Roxanne Soto) Date: Wed May 10 14:04:31 2006 Subject: [Histonet] PowerPath Tamtron In-Reply-To: Message-ID: This would all depend on which version of Tamtron you have. I know that on the upgrade, there is an advance search that you can utilize, not only by keyword, but also by doctor, like for instance if you are looking for a lymphoma control, you could look up cases by your hempath dr, as well as by key word lymph node, to narrow the search.. Roxanne Soto ______________________________________________________________ From: "Heckford, Karen - SMMC-SF" To: "Histonet (E-mail)" Subject: [Histonet] PowerPath Tamtron Date: Wed, 10 May 2006 09:51:35 -0700 >Hello Histonetters, Does anyone know of a way that I could look up past >cases by keywords? For example looking up IHC by their name. I would like >to be able to do this so I can find tissues that would make good controls. >I have searched and searched and cannot figure out how to do this. Perhaps >it does not exist. > >Thanks, >Karen Heckford HT (ASCP) CE >Lead Histology Technician >Histology/Pathololgy Department >St. Mary's Medical Center >450 Stanyan St. >San Francisco, Ca. 94117 >415-668-1000 ext. 6167 >Fax: 415-750-8123 >email: kheckfor@chw.edu >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ploykasek <@t> phenopath.com Wed May 10 13:37:19 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Wed May 10 14:12:52 2006 Subject: [Histonet] tissue processor In-Reply-To: <4C96AA62BADFD81180CB0002A5AD67FB071139C8@MAILPMA> Message-ID: I don't have any recent experience with new processors, but have heard from a trusted colleague good things about the Vision Biosystem processor. It is a conventional processor, but has quicker TAT. I don't believe this colleague is on histonet, so thought I'd mention it. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Jennifer, > I have used the Lecia at another faculty, and it had some problems, but I > had always preferred the Sakura VIP until now. I got a lemon and the service > dept is horrible, in fact we are trying to get them to replace it. Actually > the service has been so bad I would love to dump the whole company. Anyway, > sorry for venting, but I would try the thermo at this time. Did you know > that Richard Allan also has one? > Good luck, > Diane Santana > PMA > Haverhill, Mass. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From Tracey.Lenek <@t> CLS.ab.ca Wed May 10 14:16:20 2006 From: Tracey.Lenek <@t> CLS.ab.ca (Tracey.Lenek@CLS.ab.ca) Date: Wed May 10 14:16:25 2006 Subject: [Histonet] Filter papers Message-ID: <4BC300747AF87A48BCDF8E48BC2885CE02C1D73A@mail1.calgary.com> We are a large volume lab having to filter colposcopy specimens for processing. Having difficulty finding suitable filter paper to accomodate filtering approx. 80 ml of fluid per container. We have tried both Fisher and VWR and they both take far too much time to filter. Does anyone have a supplier they could recommend? Thanks in advance. Tracey CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. From RRA <@t> Stowers-Institute.org Wed May 10 14:21:37 2006 From: RRA <@t> Stowers-Institute.org (Allen, Rhonda) Date: Wed May 10 14:22:02 2006 Subject: [Histonet] tissue processor Message-ID: I would be looking at all the microwave processor's. Just my opinion. Rhonda Allen BA HT(ASCP)HTL, QIHC Histotechnology Specialist II Stowers Institute 1000 E. 50th Street Kansas City, MO 64110 816-926-4305 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Wednesday, May 10, 2006 1:37 PM To: histonet Subject: Re: [Histonet] tissue processor I don't have any recent experience with new processors, but have heard from a trusted colleague good things about the Vision Biosystem processor. It is a conventional processor, but has quicker TAT. I don't believe this colleague is on histonet, so thought I'd mention it. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Jennifer, > I have used the Lecia at another faculty, and it had some problems, > but I had always preferred the Sakura VIP until now. I got a lemon and > the service dept is horrible, in fact we are trying to get them to > replace it. Actually the service has been so bad I would love to dump > the whole company. Anyway, sorry for venting, but I would try the > thermo at this time. Did you know that Richard Allan also has one? > Good luck, Diane Santana > PMA > Haverhill, Mass. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ - This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gentras <@t> vetmed.auburn.edu Wed May 10 14:31:46 2006 From: gentras <@t> vetmed.auburn.edu (Atoska S. Gentry) Date: Wed May 10 14:31:57 2006 Subject: Fwd: [Histonet] Xylol substitutes addendnum Message-ID: <7.0.1.0.0.20060510143018.0194a270@vetmed.auburn.edu> Sorry, I forgot to mention the Hemo-De & Z-Sub can both be disposed of down the drain flushed well with H2O. Atoska >X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 >Date: Wed, 10 May 2006 11:28:24 -0500 >To: Histonet >From: "Atoska S. Gentry" >X-Scan-Signature: d81c2d972a01813ecd62f397ac6bd1bc >Cc: >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.1.5 >List-Id: For the exchange of information pertaining to histotechnology and > related fields >List-Unsubscribe: >, > > >List-Archive: >List-Post: >List-Help: >List-Subscribe: , > >Sender: histonet-bounces@lists.utsouthwestern.edu >X-Scan-Signature: 93ead75ee15f945d48515dc221efa206 >X-SA-Exim-Connect-IP: 127.0.0.1 >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >Subject: Fwd: [Histonet] Xylol substitutes >X-Spam-Checker-Version: SpamAssassin 2.64 (2004-01-11) on swlx162.swmed.edu >X-Spam-Level: >X-Spam-Status: No, hits=0.0 required=5.5 tests=none autolearn=no version=2.64 >X-SA-Exim-Version: 4.0 (built Wed, 07 Apr 2004 12:14:41 -0500) >X-SA-Exim-Scanned: Yes (on swlx162.swmed.edu) >X-spam: ESP<-123>=RBL:<-141> SHA:<15> UHA:<0> SLS:<0> BAYES:<0> SenderID:<0> > Spam Dictionary (TRU10):<0> scam_spam:<0> Obscenities > Dictionary (TRU10):<0> Scam Dictionary (TRU10):<0> Adult > Dictionary (TRU10):<0> Embed HTML Dictionary (TRU10):<0> > Float Dictionary (TRU10):<0> HTML Dictionary (TRU10):<3> URL > Real-Time Signatures:<0> Spam Dictionary 2 (TRU10):<0> > SIG: kSus5AEPh6NGuOEWBgdkkzcJYswTzLiXAPvDqYCkysjBZcu0H6qu3Ps0j3o4 > fIkZXPIZ-5zU6kcdAU-MTNLw6Qr7zhqIdxwIE_B9r3OB3pKvod89sFii0Dxo > TYZxODFTyYu-g1whIFNAAAAAAAAmTiPuQSNyrVzgtbemo0DrG> > > >Hello, both Hem-o-de and/or Mercedes Medical's Z-sub work fine for >me, but I still use a final stain dish of xylene to coverslip from. >Best wishes, Atoska > > >>Date: Wed, 10 May 2006 11:03:28 -0500 >>From: Sharon Allen >>To: "Histonet (E-mail)" >>X-Mailer: Internet Mail Service (5.5.2653.19) >>X-Scan-Signature: fa1887bb5270b51defc3a2885a1d8a92 >>Cc: >>X-BeenThere: histonet@lists.utsouthwestern.edu >>X-Mailman-Version: 2.1.5 >>List-Id: For the exchange of information pertaining to histotechnology and >> related fields >>List-Unsubscribe: >>, >> >>List-Archive: >>List-Post: >>List-Help: >>List-Subscribe: , >> >>Sender: histonet-bounces@lists.utsouthwestern.edu >>X-Scan-Signature: 9efafbcd85335e515bdf0d9adb1caaeb >>X-SA-Exim-Connect-IP: 127.0.0.1 >>X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >>Subject: [Histonet] Xylol substitutes >>X-Spam-Checker-Version: SpamAssassin 2.64 (2004-01-11) on swlx162.swmed.edu >>X-Spam-Level: >>X-Spam-Status: No, hits=0.0 required=5.5 tests=none autolearn=no version=2.64 >>X-SA-Exim-Version: 4.0 (built Wed, 07 Apr 2004 12:14:41 -0500) >>X-SA-Exim-Scanned: Yes (on swlx162.swmed.edu) >>X-spam: ESP<-125>=RBL:<-140> SHA:<15> UHA:<0> SLS:<0> BAYES:<0> SenderID:<0> >> Spam Dictionary (TRU10):<0> Obscenities Dictionary >> (TRU10):<0> Scam Dictionary (TRU10):<0> Adult Dictionary >> (TRU10):<0> Embed HTML Dictionary (TRU10):<0> Float >> Dictionary (TRU10):<0> HTML Dictionary (TRU10):<0> URL >> Real-Time Signatures:<0> Spam Dictionary 2 (TRU10):<0> >> SIG:> AAAAAAAAAAAAAAAABgdkkzcJYswTzLiXAPvDqYCkysjBZcu0NejMpAPHwz8d >> q37GXPIZ-5zU6kcdAU-MTNLw6Qr7zhqIdxwIE_B9r3OB3pKvod8Jk-n62hZy >> SoOssncAv7Xdw1uaqiNAAAAAAAAh505lYmw7IZSrFV0v2FrIe> >> >> >>Does anyone use xylol substitutes, if so how good are they? >>We do CJD testing & "the powers that be" don't like to pay for getting rid >>of contaminated xylol. I don't want to have to start testing different >>substitutes, so any help would be greatly appreciated. >>Thanks >>Sharon >>sallen@hsc.mb.ca >> >>This e-mail and/or any documents in this transmission is intended >>for the address(s) only and may contain legally privileged or >>confidential information. Any unauthorized use, disclosure, >>distribution, copying or dissemination is strictly prohibited. If >>you receive this transmission in error, please notify the sender >>immediately and return the original. >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Wed May 10 14:33:45 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed May 10 14:34:27 2006 Subject: [Histonet] Dako Message-ID: <446207D902000009000AE5ED@hcnwgwds01.hh.chs> Are others having problems with Dako Corporation in terms of getting reagents on time, products being back ordered, etc. or is it just us? Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From LuckG <@t> empirehealth.org Wed May 10 14:42:28 2006 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Wed May 10 14:42:43 2006 Subject: [Histonet] Dako Message-ID: <6BB8BC4519AAB844B174FC739A679BBC1C2FB0@IRMEXCH01.irm.inhs.org> Richard, It's not just you. If I printed what my lab purchasing agent said to me I'd be banned from the histonet for life. She has recently spent an 1.75 hours total on two phone calls with them recently and one of their recommendations to her when we couldn't get an answer as when to expect our pronase was to look for alternative vendor. So much for a new computer system. Someone didn't do their job very well at DAKO. Good luck, Greg Greg Luck Anatomic Pathology Supervisor Deaconess Med Center / EHS 800 W. 5th Ave Spokane, WA 99204 Phone 509.473.7394 Fax 509.473.7133 luckg@empirehealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Wednesday, May 10, 2006 12:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dako Are others having problems with Dako Corporation in terms of getting reagents on time, products being back ordered, etc. or is it just us? Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ander093 <@t> tc.umn.edu Wed May 10 14:49:14 2006 From: ander093 <@t> tc.umn.edu (LuAnn Anderson) Date: Wed May 10 14:49:20 2006 Subject: Fwd: [Histonet] Xylol substitutes addendnum In-Reply-To: <7.0.1.0.0.20060510143018.0194a270@vetmed.auburn.edu> References: <7.0.1.0.0.20060510143018.0194a270@vetmed.auburn.edu> Message-ID: <6.2.3.4.0.20060510144224.03b04ac8@ander093.email.umn.edu> Atoska, I wouldn't recommend flushing anything contaminated with CJD down the drain. I don't think the public health officials would be too happy about that. CJD contaminated materials (ALL solids and liquids) need to be properly disposed of and incinerated for a longer time period than ordinary waste. I also use disposable everything on these cases--forceps, dishes, containers for processing and staining, etc. LuAnn Anderson Neuropathology Lab University of Minnesota At 02:31 PM 5/10/2006, you wrote: >Sorry, I forgot to mention the Hemo-De & Z-Sub can both be disposed >of down the drain flushed well with H2O. Atoska > >>X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 >>Date: Wed, 10 May 2006 11:28:24 -0500 >>To: Histonet >>From: "Atoska S. Gentry" >>X-Scan-Signature: d81c2d972a01813ecd62f397ac6bd1bc >>Cc: >>X-BeenThere: histonet@lists.utsouthwestern.edu >>X-Mailman-Version: 2.1.5 >>List-Id: For the exchange of information pertaining to histotechnology and >> related fields >>List-Unsubscribe: >>, >> >>List-Archive: >>List-Post: >>List-Help: >>List-Subscribe: , >> >>Sender: histonet-bounces@lists.utsouthwestern.edu >>X-Scan-Signature: 93ead75ee15f945d48515dc221efa206 >>X-SA-Exim-Connect-IP: 127.0.0.1 >>X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >>Subject: Fwd: [Histonet] Xylol substitutes >>X-Spam-Checker-Version: SpamAssassin 2.64 (2004-01-11) on swlx162.swmed.edu >>X-Spam-Level: >>X-Spam-Status: No, hits=0.0 required=5.5 tests=none autolearn=no version=2.64 >>X-SA-Exim-Version: 4.0 (built Wed, 07 Apr 2004 12:14:41 -0500) >>X-SA-Exim-Scanned: Yes (on swlx162.swmed.edu) >>X-spam: ESP<-123>=RBL:<-141> SHA:<15> UHA:<0> SLS:<0> BAYES:<0> SenderID:<0> >> Spam Dictionary (TRU10):<0> scam_spam:<0> Obscenities >> Dictionary (TRU10):<0> Scam Dictionary (TRU10):<0> Adult >> Dictionary (TRU10):<0> Embed HTML Dictionary (TRU10):<0> >> Float Dictionary (TRU10):<0> HTML Dictionary (TRU10):<3> URL >> Real-Time Signatures:<0> Spam Dictionary 2 (TRU10):<0> >> SIG:> kSus5AEPh6NGuOEWBgdkkzcJYswTzLiXAPvDqYCkysjBZcu0H6qu3Ps0j3o4 >> fIkZXPIZ-5zU6kcdAU-MTNLw6Qr7zhqIdxwIE_B9r3OB3pKvod89sFii0Dxo >> TYZxODFTyYu-g1whIFNAAAAAAAAmTiPuQSNyrVzgtbemo0DrG> >> >> >>Hello, both Hem-o-de and/or Mercedes Medical's Z-sub work fine for >>me, but I still use a final stain dish of xylene to coverslip from. >>Best wishes, Atoska >> >> >>>Date: Wed, 10 May 2006 11:03:28 -0500 >>>From: Sharon Allen >>>To: "Histonet (E-mail)" >>>X-Mailer: Internet Mail Service (5.5.2653.19) >>>X-Scan-Signature: fa1887bb5270b51defc3a2885a1d8a92 >>>Cc: >>>X-BeenThere: histonet@lists.utsouthwestern.edu >>>X-Mailman-Version: 2.1.5 >>>List-Id: For the exchange of information pertaining to histotechnology and >>> related fields >>>List-Unsubscribe: >>>, >>> >>>List-Archive: >>>List-Post: >>>List-Help: >>>List-Subscribe: , >>> >>> >>>Sender: histonet-bounces@lists.utsouthwestern.edu >>>X-Scan-Signature: 9efafbcd85335e515bdf0d9adb1caaeb >>>X-SA-Exim-Connect-IP: 127.0.0.1 >>>X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >>>Subject: [Histonet] Xylol substitutes >>>X-Spam-Checker-Version: SpamAssassin 2.64 (2004-01-11) on swlx162.swmed.edu >>>X-Spam-Level: >>>X-Spam-Status: No, hits=0.0 required=5.5 tests=none autolearn=no >>>version=2.64 >>>X-SA-Exim-Version: 4.0 (built Wed, 07 Apr 2004 12:14:41 -0500) >>>X-SA-Exim-Scanned: Yes (on swlx162.swmed.edu) >>>X-spam: ESP<-125>=RBL:<-140> SHA:<15> UHA:<0> SLS:<0> BAYES:<0> SenderID:<0> >>> Spam Dictionary (TRU10):<0> Obscenities Dictionary >>> (TRU10):<0> Scam Dictionary (TRU10):<0> Adult Dictionary >>> (TRU10):<0> Embed HTML Dictionary (TRU10):<0> Float >>> Dictionary (TRU10):<0> HTML Dictionary (TRU10):<0> URL >>> Real-Time Signatures:<0> Spam Dictionary 2 (TRU10):<0> >>> SIG:>> AAAAAAAAAAAAAAAABgdkkzcJYswTzLiXAPvDqYCkysjBZcu0NejMpAPHwz8d >>> q37GXPIZ-5zU6kcdAU-MTNLw6Qr7zhqIdxwIE_B9r3OB3pKvod8Jk-n62hZy >>> SoOssncAv7Xdw1uaqiNAAAAAAAAh505lYmw7IZSrFV0v2FrIe> >>> >>> >>>Does anyone use xylol substitutes, if so how good are they? >>>We do CJD testing & "the powers that be" don't like to pay for getting rid >>>of contaminated xylol. I don't want to have to start testing different >>>substitutes, so any help would be greatly appreciated. >>>Thanks >>>Sharon >>>sallen@hsc.mb.ca >>> >>>This e-mail and/or any documents in this transmission is intended >>>for the address(s) only and may contain legally privileged or >>>confidential information. Any unauthorized use, disclosure, >>>distribution, copying or dissemination is strictly prohibited. If >>>you receive this transmission in error, please notify the sender >>>immediately and return the original. >>> >>>_______________________________________________ >>>Histonet mailing list >>>Histonet@lists.utsouthwestern.edu >>>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed May 10 14:49:56 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 10 14:50:03 2006 Subject: Fwd: [Histonet] Xylol substitutes addendnum In-Reply-To: <7.0.1.0.0.20060510143018.0194a270@vetmed.auburn.edu> Message-ID: <20060510194956.41587.qmail@web61217.mail.yahoo.com> So the almost undestructible prions can go into the sewer system? Ren? J. "Atoska S. Gentry" wrote: Sorry, I forgot to mention the Hemo-De & Z-Sub can both be disposed of down the drain flushed well with H2O. Atoska >X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 >Date: Wed, 10 May 2006 11:28:24 -0500 >To: Histonet >From: "Atoska S. Gentry" >X-Scan-Signature: d81c2d972a01813ecd62f397ac6bd1bc >Cc: >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.1.5 >List-Id: For the exchange of information pertaining to histotechnology and > related fields >List-Unsubscribe: >, > > >List-Archive: >List-Post: >List-Help: >List-Subscribe: , > >Sender: histonet-bounces@lists.utsouthwestern.edu >X-Scan-Signature: 93ead75ee15f945d48515dc221efa206 >X-SA-Exim-Connect-IP: 127.0.0.1 >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >Subject: Fwd: [Histonet] Xylol substitutes >X-Spam-Checker-Version: SpamAssassin 2.64 (2004-01-11) on swlx162.swmed.edu >X-Spam-Level: >X-Spam-Status: No, hits=0.0 required=5.5 tests=none autolearn=no version=2.64 >X-SA-Exim-Version: 4.0 (built Wed, 07 Apr 2004 12:14:41 -0500) >X-SA-Exim-Scanned: Yes (on swlx162.swmed.edu) >X-spam: ESP<-123>=RBL:<-141> SHA:<15> UHA:<0> SLS:<0> BAYES:<0> SenderID:<0> > Spam Dictionary (TRU10):<0> scam_spam:<0> Obscenities > Dictionary (TRU10):<0> Scam Dictionary (TRU10):<0> Adult > Dictionary (TRU10):<0> Embed HTML Dictionary (TRU10):<0> > Float Dictionary (TRU10):<0> HTML Dictionary (TRU10):<3> URL > Real-Time Signatures:<0> Spam Dictionary 2 (TRU10):<0> > SIG:> kSus5AEPh6NGuOEWBgdkkzcJYswTzLiXAPvDqYCkysjBZcu0H6qu3Ps0j3o4 > fIkZXPIZ-5zU6kcdAU-MTNLw6Qr7zhqIdxwIE_B9r3OB3pKvod89sFii0Dxo > TYZxODFTyYu-g1whIFNAAAAAAAAmTiPuQSNyrVzgtbemo0DrG> > > >Hello, both Hem-o-de and/or Mercedes Medical's Z-sub work fine for >me, but I still use a final stain dish of xylene to coverslip from. >Best wishes, Atoska > > >>Date: Wed, 10 May 2006 11:03:28 -0500 >>From: Sharon Allen >>To: "Histonet (E-mail)" >>X-Mailer: Internet Mail Service (5.5.2653.19) >>X-Scan-Signature: fa1887bb5270b51defc3a2885a1d8a92 >>Cc: >>X-BeenThere: histonet@lists.utsouthwestern.edu >>X-Mailman-Version: 2.1.5 >>List-Id: For the exchange of information pertaining to histotechnology and >> related fields >>List-Unsubscribe: >>, >> >>List-Archive: >>List-Post: >>List-Help: >>List-Subscribe: , >> >>Sender: histonet-bounces@lists.utsouthwestern.edu >>X-Scan-Signature: 9efafbcd85335e515bdf0d9adb1caaeb >>X-SA-Exim-Connect-IP: 127.0.0.1 >>X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >>Subject: [Histonet] Xylol substitutes >>X-Spam-Checker-Version: SpamAssassin 2.64 (2004-01-11) on swlx162.swmed.edu >>X-Spam-Level: >>X-Spam-Status: No, hits=0.0 required=5.5 tests=none autolearn=no version=2.64 >>X-SA-Exim-Version: 4.0 (built Wed, 07 Apr 2004 12:14:41 -0500) >>X-SA-Exim-Scanned: Yes (on swlx162.swmed.edu) >>X-spam: ESP<-125>=RBL:<-140> SHA:<15> UHA:<0> SLS:<0> BAYES:<0> SenderID:<0> >> Spam Dictionary (TRU10):<0> Obscenities Dictionary >> (TRU10):<0> Scam Dictionary (TRU10):<0> Adult Dictionary >> (TRU10):<0> Embed HTML Dictionary (TRU10):<0> Float >> Dictionary (TRU10):<0> HTML Dictionary (TRU10):<0> URL >> Real-Time Signatures:<0> Spam Dictionary 2 (TRU10):<0> >> SIG:>> AAAAAAAAAAAAAAAABgdkkzcJYswTzLiXAPvDqYCkysjBZcu0NejMpAPHwz8d >> q37GXPIZ-5zU6kcdAU-MTNLw6Qr7zhqIdxwIE_B9r3OB3pKvod8Jk-n62hZy >> SoOssncAv7Xdw1uaqiNAAAAAAAAh505lYmw7IZSrFV0v2FrIe> >> >> >>Does anyone use xylol substitutes, if so how good are they? >>We do CJD testing & "the powers that be" don't like to pay for getting rid >>of contaminated xylol. I don't want to have to start testing different >>substitutes, so any help would be greatly appreciated. >>Thanks >>Sharon >>sallen@hsc.mb.ca >> >>This e-mail and/or any documents in this transmission is intended >>for the address(s) only and may contain legally privileged or >>confidential information. Any unauthorized use, disclosure, >>distribution, copying or dissemination is strictly prohibited. If >>you receive this transmission in error, please notify the sender >>immediately and return the original. >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. From cgfields <@t> lexhealth.org Wed May 10 14:56:51 2006 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Wed May 10 14:52:55 2006 Subject: [Histonet] Xylol substitutes addendum Message-ID: That's not necessarily true. You have to check with your water company. Some of the substitutes are no better than xylene about disposal. If it is not miscible in water it probably has to be hauled or something like that....it happened to us. Carole Fields, HT,ASCP Pathology Supervisor Lexington Medical Center 2720 Sunset Blvd. W. Columbia, SC 29169 -----Original Message----- From: Atoska S. Gentry [mailto:gentras@vetmed.auburn.edu] Sent: Wednesday, May 10, 2006 3:32 PM To: Histonet Subject: Fwd: [Histonet] Xylol substitutes addendnum Sorry, I forgot to mention the Hemo-De & Z-Sub can both be disposed of down the drain flushed well with H2O. Atoska >X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 >Date: Wed, 10 May 2006 11:28:24 -0500 >To: Histonet >From: "Atoska S. Gentry" >X-Scan-Signature: d81c2d972a01813ecd62f397ac6bd1bc >Cc: >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.1.5 >List-Id: For the exchange of information pertaining to histotechnology and > related fields >List-Unsubscribe: >, > > >List-Archive: >List-Post: >List-Help: >List-Subscribe: , > >Sender: histonet-bounces@lists.utsouthwestern.edu >X-Scan-Signature: 93ead75ee15f945d48515dc221efa206 >X-SA-Exim-Connect-IP: 127.0.0.1 >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >Subject: Fwd: [Histonet] Xylol substitutes >X-Spam-Checker-Version: SpamAssassin 2.64 (2004-01-11) on swlx162.swmed.edu >X-Spam-Level: >X-Spam-Status: No, hits=0.0 required=5.5 tests=none autolearn=no version=2.64 >X-SA-Exim-Version: 4.0 (built Wed, 07 Apr 2004 12:14:41 -0500) >X-SA-Exim-Scanned: Yes (on swlx162.swmed.edu) >X-spam: ESP<-123>=RBL:<-141> SHA:<15> UHA:<0> SLS:<0> BAYES:<0> SenderID:<0> > Spam Dictionary (TRU10):<0> scam_spam:<0> Obscenities > Dictionary (TRU10):<0> Scam Dictionary (TRU10):<0> Adult > Dictionary (TRU10):<0> Embed HTML Dictionary (TRU10):<0> > Float Dictionary (TRU10):<0> HTML Dictionary (TRU10):<3> URL > Real-Time Signatures:<0> Spam Dictionary 2 (TRU10):<0> > SIG: kSus5AEPh6NGuOEWBgdkkzcJYswTzLiXAPvDqYCkysjBZcu0H6qu3Ps0j3o4 > fIkZXPIZ-5zU6kcdAU-MTNLw6Qr7zhqIdxwIE_B9r3OB3pKvod89sFii0Dxo > TYZxODFTyYu-g1whIFNAAAAAAAAmTiPuQSNyrVzgtbemo0DrG> > > >Hello, both Hem-o-de and/or Mercedes Medical's Z-sub work fine for >me, but I still use a final stain dish of xylene to coverslip from. >Best wishes, Atoska > > >>Date: Wed, 10 May 2006 11:03:28 -0500 >>From: Sharon Allen >>To: "Histonet (E-mail)" >>X-Mailer: Internet Mail Service (5.5.2653.19) >>X-Scan-Signature: fa1887bb5270b51defc3a2885a1d8a92 >>Cc: >>X-BeenThere: histonet@lists.utsouthwestern.edu >>X-Mailman-Version: 2.1.5 >>List-Id: For the exchange of information pertaining to histotechnology and >> related fields >>List-Unsubscribe: >>, >> >>List-Archive: >>List-Post: >>List-Help: >>List-Subscribe: , >> >>Sender: histonet-bounces@lists.utsouthwestern.edu >>X-Scan-Signature: 9efafbcd85335e515bdf0d9adb1caaeb >>X-SA-Exim-Connect-IP: 127.0.0.1 >>X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >>Subject: [Histonet] Xylol substitutes >>X-Spam-Checker-Version: SpamAssassin 2.64 (2004-01-11) on swlx162.swmed.edu >>X-Spam-Level: >>X-Spam-Status: No, hits=0.0 required=5.5 tests=none autolearn=no version=2.64 >>X-SA-Exim-Version: 4.0 (built Wed, 07 Apr 2004 12:14:41 -0500) >>X-SA-Exim-Scanned: Yes (on swlx162.swmed.edu) >>X-spam: ESP<-125>=RBL:<-140> SHA:<15> UHA:<0> SLS:<0> BAYES:<0> SenderID:<0> >> Spam Dictionary (TRU10):<0> Obscenities Dictionary >> (TRU10):<0> Scam Dictionary (TRU10):<0> Adult Dictionary >> (TRU10):<0> Embed HTML Dictionary (TRU10):<0> Float >> Dictionary (TRU10):<0> HTML Dictionary (TRU10):<0> URL >> Real-Time Signatures:<0> Spam Dictionary 2 (TRU10):<0> >> SIG:> AAAAAAAAAAAAAAAABgdkkzcJYswTzLiXAPvDqYCkysjBZcu0NejMpAPHwz8d >> q37GXPIZ-5zU6kcdAU-MTNLw6Qr7zhqIdxwIE_B9r3OB3pKvod8Jk-n62hZy >> SoOssncAv7Xdw1uaqiNAAAAAAAAh505lYmw7IZSrFV0v2FrIe> >> >> >>Does anyone use xylol substitutes, if so how good are they? >>We do CJD testing & "the powers that be" don't like to pay for getting rid >>of contaminated xylol. I don't want to have to start testing different >>substitutes, so any help would be greatly appreciated. >>Thanks >>Sharon >>sallen@hsc.mb.ca >> >>This e-mail and/or any documents in this transmission is intended >>for the address(s) only and may contain legally privileged or >>confidential information. Any unauthorized use, disclosure, >>distribution, copying or dissemination is strictly prohibited. If >>you receive this transmission in error, please notify the sender >>immediately and return the original. >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. From Jackie.O'Connor <@t> abbott.com Wed May 10 14:57:20 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed May 10 14:57:48 2006 Subject: [Histonet] Dako In-Reply-To: <446207D902000009000AE5ED@hcnwgwds01.hh.chs> Message-ID: It's been taking me over a week, sometimes longer to get routine items from DAKO. It took one month to get autostainer reagent vials. Jackie "Richard Cartun" Sent by: histonet-bounces@lists.utsouthwestern.edu 05/10/2006 02:33 PM To cc Subject [Histonet] Dako Are others having problems with Dako Corporation in terms of getting reagents on time, products being back ordered, etc. or is it just us? Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ander093 <@t> tc.umn.edu Wed May 10 14:59:12 2006 From: ander093 <@t> tc.umn.edu (LuAnn Anderson) Date: Wed May 10 14:59:30 2006 Subject: [Histonet] Xylol substitutes addendum In-Reply-To: References: Message-ID: <6.2.3.4.0.20060510145811.03b15670@ander093.email.umn.edu> That is true--in Minnesota, you cannot dump the substitutes--they are disposed of just like xylenes. LuAnn At 02:56 PM 5/10/2006, Carole Fields wrote: >That's not necessarily true. You have to check with your water company. >Some of the substitutes are no better than xylene about disposal. If it is >not miscible in water it probably has to be hauled or something like >that....it happened to us. >Carole Fields, HT,ASCP >Pathology Supervisor >Lexington Medical Center >2720 Sunset Blvd. >W. Columbia, SC 29169 > > > > >-----Original Message----- >From: Atoska S. Gentry [mailto:gentras@vetmed.auburn.edu] >Sent: Wednesday, May 10, 2006 3:32 PM >To: Histonet >Subject: Fwd: [Histonet] Xylol substitutes addendnum > > > >Sorry, I forgot to mention the Hemo-De & Z-Sub can both be disposed >of down the drain flushed well with H2O. Atoska > > >X-Mailer: QUALCOMM Windows Eudora Version 7.0.1.0 > >Date: Wed, 10 May 2006 11:28:24 -0500 > >To: Histonet > >From: "Atoska S. Gentry" > >X-Scan-Signature: d81c2d972a01813ecd62f397ac6bd1bc > >Cc: > >X-BeenThere: histonet@lists.utsouthwestern.edu > >X-Mailman-Version: 2.1.5 > >List-Id: For the exchange of information pertaining to histotechnology and > > related fields > >List-Unsubscribe: > >, > > > > > >List-Archive: > >List-Post: > >List-Help: > >List-Subscribe: >, > > > > >Sender: histonet-bounces@lists.utsouthwestern.edu > >X-Scan-Signature: 93ead75ee15f945d48515dc221efa206 > >X-SA-Exim-Connect-IP: 127.0.0.1 > >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu > >Subject: Fwd: [Histonet] Xylol substitutes > >X-Spam-Checker-Version: SpamAssassin 2.64 (2004-01-11) on swlx162.swmed.edu > >X-Spam-Level: > >X-Spam-Status: No, hits=0.0 required=5.5 tests=none autolearn=no >version=2.64 > >X-SA-Exim-Version: 4.0 (built Wed, 07 Apr 2004 12:14:41 -0500) > >X-SA-Exim-Scanned: Yes (on swlx162.swmed.edu) > >X-spam: ESP<-123>=RBL:<-141> SHA:<15> UHA:<0> SLS:<0> BAYES:<0> >SenderID:<0> > > Spam Dictionary (TRU10):<0> scam_spam:<0> Obscenities > > Dictionary (TRU10):<0> Scam Dictionary (TRU10):<0> Adult > > Dictionary (TRU10):<0> Embed HTML Dictionary (TRU10):<0> > > Float Dictionary (TRU10):<0> HTML Dictionary (TRU10):<3> URL > > Real-Time Signatures:<0> Spam Dictionary 2 (TRU10):<0> > > SIG: > kSus5AEPh6NGuOEWBgdkkzcJYswTzLiXAPvDqYCkysjBZcu0H6qu3Ps0j3o4 > > fIkZXPIZ-5zU6kcdAU-MTNLw6Qr7zhqIdxwIE_B9r3OB3pKvod89sFii0Dxo > > TYZxODFTyYu-g1whIFNAAAAAAAAmTiPuQSNyrVzgtbemo0DrG> > > > > > >Hello, both Hem-o-de and/or Mercedes Medical's Z-sub work fine for > >me, but I still use a final stain dish of xylene to coverslip from. > >Best wishes, Atoska > > > > > >>Date: Wed, 10 May 2006 11:03:28 -0500 > >>From: Sharon Allen > >>To: "Histonet (E-mail)" > >>X-Mailer: Internet Mail Service (5.5.2653.19) > >>X-Scan-Signature: fa1887bb5270b51defc3a2885a1d8a92 > >>Cc: > >>X-BeenThere: histonet@lists.utsouthwestern.edu > >>X-Mailman-Version: 2.1.5 > >>List-Id: For the exchange of information pertaining to histotechnology and > >> related fields > >>List-Unsubscribe: > >>, > >> > >>List-Archive: > >>List-Post: > >>List-Help: > >>List-Subscribe: >, > >> > > >>Sender: histonet-bounces@lists.utsouthwestern.edu > >>X-Scan-Signature: 9efafbcd85335e515bdf0d9adb1caaeb > >>X-SA-Exim-Connect-IP: 127.0.0.1 > >>X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu > >>Subject: [Histonet] Xylol substitutes > >>X-Spam-Checker-Version: SpamAssassin 2.64 (2004-01-11) on >swlx162.swmed.edu > >>X-Spam-Level: > >>X-Spam-Status: No, hits=0.0 required=5.5 tests=none autolearn=no >version=2.64 > >>X-SA-Exim-Version: 4.0 (built Wed, 07 Apr 2004 12:14:41 -0500) > >>X-SA-Exim-Scanned: Yes (on swlx162.swmed.edu) > >>X-spam: ESP<-125>=RBL:<-140> SHA:<15> UHA:<0> SLS:<0> BAYES:<0> >SenderID:<0> > >> Spam Dictionary (TRU10):<0> Obscenities Dictionary > >> (TRU10):<0> Scam Dictionary (TRU10):<0> Adult Dictionary > >> (TRU10):<0> Embed HTML Dictionary (TRU10):<0> Float > >> Dictionary (TRU10):<0> HTML Dictionary (TRU10):<0> URL > >> Real-Time Signatures:<0> Spam Dictionary 2 (TRU10):<0> > >> SIG: >> AAAAAAAAAAAAAAAABgdkkzcJYswTzLiXAPvDqYCkysjBZcu0NejMpAPHwz8d > >> q37GXPIZ-5zU6kcdAU-MTNLw6Qr7zhqIdxwIE_B9r3OB3pKvod8Jk-n62hZy > >> SoOssncAv7Xdw1uaqiNAAAAAAAAh505lYmw7IZSrFV0v2FrIe> > >> > >> > >>Does anyone use xylol substitutes, if so how good are they? > >>We do CJD testing & "the powers that be" don't like to pay for getting rid > >>of contaminated xylol. I don't want to have to start testing different > >>substitutes, so any help would be greatly appreciated. > >>Thanks > >>Sharon > >>sallen@hsc.mb.ca > >> > >>This e-mail and/or any documents in this transmission is intended > >>for the address(s) only and may contain legally privileged or > >>confidential information. Any unauthorized use, disclosure, > >>distribution, copying or dissemination is strictly prohibited. If > >>you receive this transmission in error, please notify the sender > >>immediately and return the original. > >> > >>_______________________________________________ > >>Histonet mailing list > >>Histonet@lists.utsouthwestern.edu > >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_________________________________ >This email and any files transmitted with it may contain PRIVILEGED or >CONFIDENTIAL information and may be read or used only by the intended >recipient. If you are not the intended recipient of the email or any of its >attachments, please be advised that you have received this email in error >and that any use, dissemination, distribution, forwarding, printing, or >copying of this email or any attached files is strictly prohibited. If you >have received this email in error, please immediately purge it and all >attachments and notify the sender by reply email or contact the sender at >the number listed above if one is provided. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LRaff <@t> lab.uropartners.com Wed May 10 15:07:55 2006 From: LRaff <@t> lab.uropartners.com (Lester Raff) Date: Wed May 10 15:08:01 2006 Subject: [Histonet] Re: Slide labeler Message-ID: <5DA1CA5D0B98A84985B545A24423B822A88D@UPLAB01.uplab.local> Kim: We are a small lab (100-200 slides/day) and use the Slide and Cassette Printers from Surgipath. They are simple to operate and do the job just fine. Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory ph: 708-486-0076 fax: 708-486-0080 From Charlene.Henry <@t> STJUDE.ORG Wed May 10 15:16:26 2006 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Wed May 10 15:16:34 2006 Subject: [Histonet] decal. . Message-ID: <5CB39BCA5724F349BCB748675C6CA1A2099A1C63@SJMEMXMB02.stjude.sjcrh.local> We also have the same microwave processor and I would be interested in these protocols also. Charlene -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Wednesday, May 10, 2006 12:31 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] decal. . Does anyone use the microwave processor for decalcification of specimens? If so, I would appreciate any protocols in this area. We have the Thermo Tissue-Wave which we use daily for processing small biopsies and cell blocks. Thanks ahead of time for any and all information either through my direct Email or this venue!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmcardle <@t> ebsciences.com Wed May 10 15:51:02 2006 From: pmcardle <@t> ebsciences.com (Phil McArdle) Date: Wed May 10 15:51:14 2006 Subject: [Histonet] decal. Message-ID: <44625236.6040901@ebsciences.com> Hi: If you don't mind a "vendor" response, check out Kok & Boon's "Microwave Cookbook for Microscopists," page 184, or the "Library" section of our website: http://www.ebsciences.com/papers/microwave.htm#Decalcification Best regards, Phil McArdle -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. Tel: 800.992.9037 x 341 Cell: 860.597.6796 Fax: 860.653.0422 PMcardle@ebsciences.com www.ebsciences.com "ADDING BRILLIANCE TO YOUR VISION" "I hate quotations. Tell me what you know." Ralph Waldo Emerson Webb, Dorothy L wrote: > Does anyone use the microwave processor for decalcification of > specimens? If so, I would appreciate any protocols in this area. We > have the Thermo Tissue-Wave which we use daily for processing small > biopsies and cell blocks. > > Thanks ahead of time for any and all information either through my > direct Email or this venue!! > ________________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they > are addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, > please be advised that you have received this e-mail in error and that > any use, dissemination, forwarding, printing, or copying of this > e-mail is strictly prohibited. > > If you have received this e-mail in error, please immediately notify > the HealthPartners Support Center by telephone at (952) 967-6600. You > will be reimbursed for reasonable costs incurred in notifying us. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ploykasek <@t> phenopath.com Wed May 10 16:21:43 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Wed May 10 16:21:53 2006 Subject: [Histonet] (no subject) In-Reply-To: <6F434E27D5DE944B9E898984CADDD149043A67@exchmail.CMH.Internal> Message-ID: Hi Debby. I'll do my best to answer your question. When we receive a new lot of antibody we do a titer check bracketing the current in-use titer. For example if the current titer is 1:50, we would titer check the new lot at 1:25, 1:50, and 1:100. We use the control tissue currently in use - this way we can compare the new lot of antibody with the current lot on the same tissue (I think that can be important). We use the current pretreatment. We compare it with our current antibody & QC, pick a titer (sometimes we have to do more than just our bracketing titer). Document titer on the new lot data sheet, enter titer into our computer data base & are ready to go. Just an fyi - we have little stickers we print off & put on the data sheets. The stickers have a space for date received, lot #, titer, and date lot is put in use. I hope this helps. There are a few more details, if you'd like more just let me know. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Hi All, > > > > Are there any labs running side by side slides on old lot and new lot > antibodies every time you get a new lot of antibody? Please be very > specific on your answers. > > > > Thanks! > > > > Debby R. Grant HT (ASCP) QIHC > > Histology Coordinator > > The Children's Mercy Hospital > > 2401 Gillham Road > > KC, Mo 64108 > > Lab(816)234-3827 Fax(816)802-1492 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From meligroc <@t> zgi.com Wed May 10 16:32:01 2006 From: meligroc <@t> zgi.com (CRME (Criss Meligro)) Date: Wed May 10 16:32:15 2006 Subject: [Histonet] (no subject) Message-ID: <746E68D5CBB205409B12EC32A61EE401D91A7B@ned.zgi.com> We have one of the old TechMate IHC "football fields" that is working great but we want to find slide holder! Any suggestions would be appreciated. Holds 60 capillary gap slides/rack. THANKS Criss Meligro From AnthonyH <@t> chw.edu.au Wed May 10 18:21:31 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed May 10 18:50:45 2006 Subject: [Histonet] IHC on Cheek cells Message-ID: Rabbit antibodies are notorious for having a natural antibody population directed against human keratins. I would recommend absorbing the tissues with human keratin extracts. There was a paper some time ago (?10 years) that described this. Unfortunately I can't find it (These days I have problems finding my way home!!) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of yan gao Sent: Thursday, 11 May 2006 1:46 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC on Cheek cells Hi, Histonet. I am staining phosoph-MEK on cheek cells. Here is the way how I collected cheek cells. I used cotton tips scrape inside of human mouth. then I soak the tip into the fixative offered by Thermo shandon for cytospin. Then I cytospined cells to slides. While I stained pMEK, I brought a rabbit IgG with it for negative control. I got a positive stain on cheek cells. Cells were very sticky. Anyone has IHC experience on buccal cells would like share with me, I really appreciated. Thanks a lot. Yan Gao Novartis _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From AnthonyH <@t> chw.edu.au Wed May 10 18:23:19 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed May 10 18:50:48 2006 Subject: [Histonet] IHC on Cheek cells Message-ID: Yep, Though I would use a cell culture fluid like Hanks rather than PBS. Seems to preserve the cells better. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Thursday, 11 May 2006 2:46 AM To: yan gao; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] IHC on Cheek cells We used wooden tongue depressors to scrape cheeks for buccal smears - that way you do not have cells trapped in cotton fibers. There are scrapers, plastic that may work better, you can still resuspend the cells off the ends - agitate the tubes containing scraper with a vortex mixer to let cells go into PBS. I think you could cytospin the cells onto slides easily. At 09:45 AM 5/10/2006, you wrote: > Hi, Histonet. > > I am staining phosoph-MEK on cheek cells. Here is the way how I > collected cheek cells. I used cotton tips scrape inside of human > mouth. then I soak the tip into the fixative offered by Thermo > shandon for cytospin. Then I cytospined cells to slides. > While I stained pMEK, I brought a rabbit IgG with it for negative > control. I got a positive stain on cheek cells. Cells were very > sticky. > Anyone has IHC experience on buccal cells would like share with me, I > really appreciated. Thanks a lot. > > Yan Gao > > Novartis >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From mgdelaware <@t> comcast.net Wed May 10 19:47:36 2006 From: mgdelaware <@t> comcast.net (Marian Powers) Date: Wed May 10 19:48:43 2006 Subject: [Histonet] Immuno. for Cat Scratch Message-ID: <000801c67494$7f24fc70$3227c847@D7XQNX91> Wondering if there is an antibody for Cat Scratch Bacillus? Thanks in advance, Marian From jnocito <@t> satx.rr.com Wed May 10 21:32:31 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed May 10 21:32:38 2006 Subject: [Histonet] Dako References: <446207D902000009000AE5ED@hcnwgwds01.hh.chs> Message-ID: <002801c674a3$27e15d50$1bb30b43@yourxhtr8hvc4p> I think something is going on that we don't know. Maybe another merger or buyout. I've used Dako's products since for 20 years and never have I had a problem getting their reagents, up until about a year ago. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Richard Cartun" To: Sent: Wednesday, May 10, 2006 2:33 PM Subject: [Histonet] Dako > Are others having problems with Dako Corporation in terms of getting > reagents on time, products being back ordered, etc. or is it just us? > > Richard > > Richard W. Cartun, Ph.D. > Director, Immunopathology & Histology > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-0174 Fax > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.392 / Virus Database: 268.5.5/335 - Release Date: 5/9/2006 > > From jnocito <@t> satx.rr.com Wed May 10 21:40:26 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed May 10 21:40:29 2006 Subject: [Histonet] tissue processor References: <4C96AA62BADFD81180CB0002A5AD67FB071139C8@MAILPMA> Message-ID: <00a701c674a4$42b93390$1bb30b43@yourxhtr8hvc4p> here's my three cents. We have a 1991 VIP 2000 and a 2002 VIP 5, both are working pretty well. I have a biomed company that keeps these machines running like champs. Once in a while, the older VIP's pump will go out, but other than that, work horses. I guess that's why Sakura stopped servicing the old VIPs, they couldn't sell any of the new ones. I just know I just P.Oed somebody, so being close to Friday, flame me baby, flame me. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Santana, Diane" To: Sent: Wednesday, May 10, 2006 12:02 PM Subject: [Histonet] tissue processor > Jennifer, > I have used the Lecia at another faculty, and it had some problems, but I > had always preferred the Sakura VIP until now. I got a lemon and the > service > dept is horrible, in fact we are trying to get them to replace it. > Actually > the service has been so bad I would love to dump the whole company. > Anyway, > sorry for venting, but I would try the thermo at this time. Did you know > that Richard Allan also has one? > Good luck, > Diane Santana > PMA > Haverhill, Mass. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.392 / Virus Database: 268.5.5/335 - Release Date: 5/9/2006 > > From anh2006 <@t> med.cornell.edu Wed May 10 22:35:16 2006 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Wed May 10 22:34:30 2006 Subject: [Histonet] Dako In-Reply-To: <6BB8BC4519AAB844B174FC739A679BBC1C2FB0@IRMEXCH01.irm.inhs.org> References: <6BB8BC4519AAB844B174FC739A679BBC1C2FB0@IRMEXCH01.irm.inhs.org> Message-ID: We also have been having problems. I just switched to biotin blocking kits from Vector and DAB+ from Zymed b/c of all the backordering and no one answers the phone etc. Plus it's been impossible to get in touch with a sales rep. I can't bear the thought though of life without EnVision+ so I hope all will be better soon!! Andrea > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard >Cartun >Sent: Wednesday, May 10, 2006 12:34 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Dako > >Are others having problems with Dako Corporation in terms of getting >reagents on time, products being back ordered, etc. or is it just us? > >Richard > >Richard W. Cartun, Ph.D. >Director, Immunopathology & Histology >Assistant Director, Anatomic Pathology >Hartford Hospital >80 Seymour Street >Hartford, CT 06102 >(860) 545-1596 >(860) 545-0174 Fax > -- From nfournier <@t> sasktel.net Thu May 11 02:42:50 2006 From: nfournier <@t> sasktel.net (Neil Fournier) Date: Thu May 11 02:45:08 2006 Subject: [Histonet] wrinkling of rat brain tissue on subbed slides Message-ID: <000601c674ce$81da9a30$89c8c5d8@NEIL6FC9056E3D> I have been having difficulty lately with rat brain tissue (40 to 50 micron thick) mounted on gelatin coated slides. We have noticed that several sections will often show wrinkling and folds along the sides of the cortex. In some cases, the tissue has severe folds and wrinkling throughout making them unusable for quantification. We believe the problem emerges during our cresyl violet staining, but we have made up new solution and still have the same problems. The sections are not falling off but they are typically wrinkled and folded along the sides; however, I must mention that it isn't every section on every slide showing the problem. The folding is extremely annoying since we are interested in quantifying amygdaloid regions and piriform cortex We originally thought that perhaps the slides were too old (they were subbed in July 2004); however, we have purchased new slides and have the same problem. We have never stored our slides in the fridge, but I am thinking that we should be begin to adopt this protocol (Is there a specific temperature that the slides should be kept at? And does one mount tissue on slides just taken out of a fridge or should the slides equilibrate at room temperature before mounting?). Our basic protocol is the following: mount sections on subbed slides (We use 0.5% gelatin and 0.05% chromium potassium sulphate) and let dry minimal 24 hrs before cresyl violet staining. We simply place the slides on a tray lying flat with a paper towel placed on top. After 24 hrs, we then stain the slides with cresyl violet. Our cresyl violet protocol is as follows: 1) distilled H2O (for 1 min), 2) 100% EtOH (3 min), 3) 95% EtOH (3 min), 4 70% ETOH (3 min), 5) distilled H2O (3 min), 6) 0.1% cresyl violet (5 - 10 min) made from 1% cresyl violet stock solution, 7) distilled H20 (rinse), 8) distilled H2O (rinse), 9) 3% glacial acetic acid (4 quick dips), 10) distilled H2O (rinse), 11) 70% ETOH (1 min), 12) 95% ETOH (1 min), 13) 100% ETOH (1 min), 14) xylene (at least 5 min). (Perhaps there is something wrong with this protocol or steps. Any potential suggestions are welcomed). Has anyone ever encountered this problem before and have potential solutions. Any help is appreciated, Neil Fournier From DDDeltour <@t> mar.med.navy.mil Thu May 11 05:27:41 2006 From: DDDeltour <@t> mar.med.navy.mil (Deltour, Douglas D. (HM2)) Date: Thu May 11 05:28:15 2006 Subject: [Histonet] tissue processor Message-ID: <3F500F8B416C554EBB21FF16642F72E959CE84@marxchg03.mar.med.navy.mil> I can tell you first hand that the Vision Biosystem processor is ... not optimal. The processor was not set-up correctly and when it was breaking down the tech-support was horrible. If you were getting error codes on every run and tech support said "just ignore them"... what would you do? D -----Original Message----- From: Patti Loykasek [mailto:ploykasek@phenopath.com] Sent: Wednesday, May 10, 2006 2:37 PM To: histonet Subject: Re: [Histonet] tissue processor I don't have any recent experience with new processors, but have heard from a trusted colleague good things about the Vision Biosystem processor. It is a conventional processor, but has quicker TAT. I don't believe this colleague is on histonet, so thought I'd mention it. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Jennifer, > I have used the Lecia at another faculty, and it had some problems, but I > had always preferred the Sakura VIP until now. I got a lemon and the service > dept is horrible, in fact we are trying to get them to replace it. Actually > the service has been so bad I would love to dump the whole company. Anyway, > sorry for venting, but I would try the thermo at this time. Did you know > that Richard Allan also has one? > Good luck, > Diane Santana > PMA > Haverhill, Mass. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Thu May 11 05:47:19 2006 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Thu May 11 05:47:26 2006 Subject: [Histonet] double immunostaining Message-ID: Information please on the preferred kits/systems used for double/triple immunostaining(non-fluorescence), probably using combinations of anti-mouse and rabbit primaries, on paraffin sections. Many thanks Richard Edwards MRC TOXICOLOGY UNIT LEICESTER U.K. From PKamalavenkatesh <@t> wockhardtin.com Thu May 11 06:22:15 2006 From: PKamalavenkatesh <@t> wockhardtin.com (PKamalavenkatesh@wockhardtin.com) Date: Thu May 11 06:16:12 2006 Subject: [Histonet] safranin o staining- glycosaminoglycans-cartilage Message-ID: Dear Histonetters, I am in need of the procedure for staining of glycosaminoglycans in articular cartilage. The literature search indicated the use of safranin o staining for the characterization of glycosaminoglycans. If any body have good experience in this procedure and able to share their protocol. I have already ordered a paper that deals with this. Method of Histomorphometric Assessment of Glycosaminoglycans in Articular Cartilage Journal of Orthopaedic Research Vol 15, 670-674. 1977 Choji Shimizu, Richard D. Coutts, Robert M. Healey Toshikazu Kubo, Yasusuke Hirasawa and David Amiel. REGARDS Dr.P.Kamalavenkatesh Drug Discovery- Biology Pre clinical Safety Assessment Wockhardt Research Center, Aurangabad, India. E.mail : Pkamalavenkatesh@wockhardtin.com Please Visit our New Corporate Web Site www.wockhardt.com --------------------- Disclaimer ------------------------------------------------------------------------------ Information transmitted by this E-MAIL is proprietary to Wockhardt Ltd. and/ or its Group Companies and/or,its Customers and is intended for use only by the individual or entity to which it is addressed, and may contain information that is privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. --------------------------------------------------------------------------------------------------------------- From Julie.Sanders <@t> va.gov Thu May 11 06:28:06 2006 From: Julie.Sanders <@t> va.gov (Sanders, Julie, VHACIN) Date: Thu May 11 06:28:13 2006 Subject: [Histonet] RE: Histonet Digest, Vol 30, Issue 13 Message-ID: <2D4ACE41DEFE93428F23D77988EFBCB50E5FBE@VHAV10MSGA2.v10.med.va.gov> From: "Heckford, Karen - SMMC-SF" To: "Histonet (E-mail)" Subject: [Histonet] PowerPath Tamtron Date: Wed, 10 May 2006 09:51:35 -0700 >Hello Histonetters, Does anyone know of a way that I could look up past >cases by keywords? For example looking up IHC by their name. I would like >to be able to do this so I can find tissues that would make good controls. >I have searched and searched and cannot figure out how to do this. Perhaps >it does not exist. > Hi Karen, When I look up tissues for controls I use snomed codes. I don't know if you have this capability, but it works really well. You can look by tissue type and diagnosis. Julie Sanders, BA, HT(ASCP) Supervisor, Anatomic Pathology VAMC Cincinnati, Ohio From Janet.Bonner <@t> FLHOSP.ORG Thu May 11 06:38:22 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Thu May 11 06:39:04 2006 Subject: [Histonet] Dako References: <002801c674a3$27e15d50$1bb30b43@yourxhtr8hvc4p> Message-ID: Do you think Thermo/Shandon-Fisher bought them!!??!! ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Joe Nocito Sent: Wed 5/10/2006 10:32 PM To: Richard Cartun; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Dako I think something is going on that we don't know. Maybe another merger or buyout. I've used Dako's products since for 20 years and never have I had a problem getting their reagents, up until about a year ago. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Richard Cartun" To: Sent: Wednesday, May 10, 2006 2:33 PM Subject: [Histonet] Dako > Are others having problems with Dako Corporation in terms of getting > reagents on time, products being back ordered, etc. or is it just us? > > Richard > > Richard W. Cartun, Ph.D. > Director, Immunopathology & Histology > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-0174 Fax > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.392 / Virus Database: 268.5.5/335 - Release Date: 5/9/2006 > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Thu May 11 07:09:32 2006 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Thu May 11 07:09:55 2006 Subject: [Histonet] DabOut System Message-ID: Does anyone have experience using the DabOut System from Triangle Biomedical Sciences? It filters out the DAB from your waste? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From Karen.Heckford <@t> CHW.edu Thu May 11 07:15:44 2006 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Thu May 11 07:16:17 2006 Subject: [Histonet] Powerpath Tamtron Message-ID: Thank you everyone for your help in my Powerpath dilemma. I have a older version 7.40. I will be working with IMPAC to solve my problem. Thanks for all your input, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathololgy Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu From ABonaiuto <@t> sarapath.com Thu May 11 07:18:50 2006 From: ABonaiuto <@t> sarapath.com (Alice Bonaiuto) Date: Thu May 11 07:18:34 2006 Subject: [Histonet] Dako Message-ID: <211AEAE1E7C4974BB8B62A7BF8E4FF80073622@webber_nt.sarapath.com> Richard. We've also been back ordered since beginning of April for DAB+ Alice Alice Bonaiuto ICH Research Supervisor Sarasota Pathology Sarasota, FL 941.362.8921 -----Original Message----- From: Richard Cartun [mailto:Rcartun@harthosp.org] Sent: Wednesday, May 10, 2006 3:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dako Are others having problems with Dako Corporation in terms of getting reagents on time, products being back ordered, etc. or is it just us? Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************************************************** SARASOTA PATHOLOGY CONFIDENTIALITY NOTICE: The information contained in this E-Mail message may be privileged, confidential, and protected under applicable law and is intended solely for the use of the individual or en- tity to whom it is addressed. If you are not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. It is strictly prohi- bited from using the internet email system to send messages with patient identifiable information per HIPAA Privacy regulations and Sarasota Patho- logy policy. If you have received this communication in error, please notify the sender immediately and delete this message. **************************************************************************** From JGREWE <@t> OhioHealth.com Thu May 11 07:30:39 2006 From: JGREWE <@t> OhioHealth.com (JGREWE@OhioHealth.com) Date: Thu May 11 07:30:46 2006 Subject: [Histonet] Open Positions in Columbus, Ohio Message-ID: We have 2 full time position openings for HT (ASCP) at Riverside Methodist Hospital in Columbus, Ohio Rsponsibilities include cutting, embedding and special stains for routine surgical specimens Our website for online applications is www.OhioHealth.com Resume's can be sent to: Riverside Methodist Hospital Histology Laboratory Jacquelyn Grewe, Histology Supervisor 3535 Olentangy River Road Columbus, Ohio 43214 jgrewe@OhioHealth.com From Lynne.Bell <@t> hitchcock.org Thu May 11 07:45:29 2006 From: Lynne.Bell <@t> hitchcock.org (Bell, Lynne) Date: Thu May 11 07:45:36 2006 Subject: [Histonet] Dako Message-ID: We were also having problems, however, all of my backordered items have finally arrived. Perhaps just writing of this problem on the Histonet will help, though. A few weeks ago I mentioned on the Histonet that I was having a problem with backordered items. My account manager promptly contacted me and came to visit. Lynne A. Bell, HT (ASCP) Central Vermont Medical Center 130 Fisher Road Barre, VT 05641 802-371-4122 From GDawson <@t> dynacaremilwaukee.com Thu May 11 08:06:49 2006 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Thu May 11 08:06:57 2006 Subject: [Histonet] Dako Message-ID: All, Dako has been undergoing an Oracle computer system change & I've noticed the bugz myself. I am currently undergoing a changeover to Cerner Millenium in my lab so I may have more sympathy for them than most because we're in the same foxhole. As with my lab, I'm sure things will run much smoother a little ways down the road. With Sympathy, Glen Dawson BS, HT & QIHC IHC Manager Milwaukee, WI From godsgirlnow <@t> msn.com Thu May 11 08:39:13 2006 From: godsgirlnow <@t> msn.com (Roxanne Soto) Date: Thu May 11 08:39:20 2006 Subject: [Histonet] double immunostaining In-Reply-To: Message-ID: I do double staining all day. A good referense to contact is Chris or Fatima at Biocare. They sell all you need and will show you how to use and even have an immuno stainer that will do them. Roxanne Soto ______________________________________________________________ From: "Edwards, R.E." To: "histonet" Subject: [Histonet] double immunostaining Date: Thu, 11 May 2006 11:47:19 +0100 > >Information please on the preferred kits/systems used for double/triple immunostaining(non-fluorescence), probably using combinations of anti-mouse and rabbit primaries, on paraffin sections. > > Many thanks > Richard Edwards > &n bsp; MRC TOXICOLOGY UNIT > LEICESTER > U.K. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Thu May 11 09:02:24 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu May 11 09:03:06 2006 Subject: [Histonet] Immuno. for Cat Scratch In-Reply-To: <000801c67494$7f24fc70$3227c847@D7XQNX91> References: <000801c67494$7f24fc70$3227c847@D7XQNX91> Message-ID: <44630BB002000009000AE681@hcnwgwds01.hh.chs> Yes, but, in my experience, it is a very difficult IP stain to interpret and unless you have significant experience with it you should probably refer cases to an expert. Sampling, duration of the infection, and treatment with antibiotics all affect immunoreactivity. The monoclonal antibody that we use (clone H2A10) is sold by Novus Biologicals in Littleton, CO. BioCare sells the same antibody. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Marian Powers" 05/10/06 8:47 PM >>> Wondering if there is an antibody for Cat Scratch Bacillus? Thanks in advance, Marian _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> Stowers-Institute.org Thu May 11 09:17:12 2006 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Thu May 11 09:17:47 2006 Subject: [Histonet] Re: double immunostaining Message-ID: Dear Richard, Kits are not needed to design and successfully do doublestaining. I recommend you buy Chris van der Loos' book on the subject, and therein lies pretty much all the information you will need. ISBN: 038791594X You should be able to get this through Amazon.com and other online book vendors. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64133 From Vickroy.Jim <@t> mhsil.com Thu May 11 09:28:50 2006 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Thu May 11 09:29:12 2006 Subject: [Histonet] Marking prostate biopsies Message-ID: We are looking for a way to better mark our small needle biopsies such as breast cores and prostate biopsies so that they are more easily seen after processing. Right now the embedder has some trouble identifying the cores and in turn the histotech often has problems seeing the cores in the paraffin block. We are doing levels on these cases but find that often we need recuts because we are not into the block enough. I have heard of some techs who have used a little eosin in their 70% ETOH, which helped the techs identify the cores. I am reluctant to use eosin on my automated processors in the 70% ETOH however. We tried dipping the blocks in eosin tinted formalin at the grossing stations. The eosin leached out and did no good. Does anyone have any good ideas? I do know that some send eosin tinted formalin to sites that are doing these biopsies. I don't see that as an option for us. Thanks for your help. James R. Vickroy Supervisor - MMC Surgical Pathology 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From HParker <@t> Skaggs.Net Thu May 11 09:31:59 2006 From: HParker <@t> Skaggs.Net (Parker, Helayne) Date: Thu May 11 09:32:14 2006 Subject: [Histonet] Anatech Hp Stain Message-ID: <2FABC6145388F24EBA8CBD6EC165BCCB02AFBA69@mail1-schc.skaggs.net> Hi All, We have been having trouble with the Hp stain. We get green instead of bright yellow. Depending on the green we can easily see the bacteria and other times not. Dr. is complaining as very inconsistent. We have tried the product insert suggestion to no great avail. Any suggestions, Helayne Parker, HT (A.S.C.P.) Histology Section Head Skaggs Community Health Center Branson, Missouri From kemlo.rogerson <@t> waht.swest.nhs.uk Thu May 11 09:36:35 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu May 11 09:36:22 2006 Subject: [Histonet] Marking prostate biopsies Message-ID: I used to dip breast biopsy cores into very dilute alcoholic eosin just for a few seconds and they came through the Processors OK. You could post fix after formalin with a picric acid fixative but then you'd have to wash it out. If you decide to use eosin then don't use a single receptacle for dipping the biopsies into; cross over. I used to put a few drops into the upturned lid of the biopsy pot and then dip the cores in, one by one, then put into the cassette. I used then to forget and put the lid on the pot and cover myself in eosin, and the floor, and my coat, and the MLA next to me. But better that then give someone a false positive result. A bit red in the face..... Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 The real does not die, the unreal never lived. --Nisargadatta Maharaj This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Diane.Gladney <@t> se.amedd.army.mil Thu May 11 09:36:02 2006 From: Diane.Gladney <@t> se.amedd.army.mil (Gladney, Diane C Ms MACH) Date: Thu May 11 09:36:49 2006 Subject: [Histonet] Marking prostate biopsies Message-ID: <4D55B2E997EFAE4DA6081DDE100B8302DE91FD@amedmlsermc133> I use Eosin in my last 100% Ethanol on my tissue processor. It works great for the small biopsies of other tissue also. We have not had any problems seeing these small biopsies or needle core biopsies since I started doing this. Diane Diane C. Gladney, HT (ASCP) Supervisor, Histology Dept. Moncrief Army Community Hospital Dept. of Pathology 4500 Stuart St. FT. Jackson, SC 29207 Email: diane.gladney@se.amedd.army.mil Phone: 803-751-2530 FAX: 803-751-7829 DSN: 734-2530 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Thursday, May 11, 2006 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Marking prostate biopsies We are looking for a way to better mark our small needle biopsies such as breast cores and prostate biopsies so that they are more easily seen after processing. Right now the embedder has some trouble identifying the cores and in turn the histotech often has problems seeing the cores in the paraffin block. We are doing levels on these cases but find that often we need recuts because we are not into the block enough. I have heard of some techs who have used a little eosin in their 70% ETOH, which helped the techs identify the cores. I am reluctant to use eosin on my automated processors in the 70% ETOH however. We tried dipping the blocks in eosin tinted formalin at the grossing stations. The eosin leached out and did no good. Does anyone have any good ideas? I do know that some send eosin tinted formalin to sites that are doing these biopsies. I don't see that as an option for us. Thanks for your help. James R. Vickroy Supervisor - MMC Surgical Pathology 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Thu May 11 09:41:16 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu May 11 09:41:23 2006 Subject: [Histonet] Immuno. for Cat Scratch Message-ID: <09C945920A6B654199F7A58A1D7D1FDE017176F7@lsexch.lsmaster.lifespan.org> Antibody against Bartonella henselae, the agent of cat scratch disease, exists, because some commercial labs offer the immunofluorescence technique. However, I don't know of a source for the antibody. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > Marian Powers > Sent: Wednesday, May 10, 2006 5:47 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Immuno. for Cat Scratch > > Wondering if there is an antibody for Cat Scratch Bacillus? > > Thanks in advance, > > Marian > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From funderwood <@t> mcohio.org Thu May 11 09:51:29 2006 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Thu May 11 09:52:02 2006 Subject: [Histonet] Marking prostate biopsies Message-ID: I have used Mrs. Stewarts Bluing Agent. It's a product used for whitening clothes. We would dip prostate biopsies, or place a drop onto the biopsy, at the grossing table. Blot off any excess,wrap in lens paper, and process. The biopsies would be colored a highly visible blue. Fred >>> "Vickroy, Jim" 05/11/06 10:28AM >>> We are looking for a way to better mark our small needle biopsies such as breast cores and prostate biopsies so that they are more easily seen after processing. Right now the embedder has some trouble identifying the cores and in turn the histotech often has problems seeing the cores in the paraffin block. We are doing levels on these cases but find that often we need recuts because we are not into the block enough. I have heard of some techs who have used a little eosin in their 70% ETOH, which helped the techs identify the cores. I am reluctant to use eosin on my automated processors in the 70% ETOH however. We tried dipping the blocks in eosin tinted formalin at the grossing stations. The eosin leached out and did no good. Does anyone have any good ideas? I do know that some send eosin tinted formalin to sites that are doing these biopsies. I don't see that as an option for us. Thanks for your help. James R. Vickroy Supervisor - MMC Surgical Pathology 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jamie.erickson <@t> abbott.com Thu May 11 10:03:24 2006 From: jamie.erickson <@t> abbott.com (Jamie E Erickson) Date: Thu May 11 10:03:31 2006 Subject: [Histonet] Cryopreservation dry ice vs LN2 isopentane Message-ID: Hello histonetters, I am hoping that someone out there can help me with a cryopreservation dilemma. I am in a research pathology lab and we have two schools of cryopreservation here, some researchers use dry ice isopentane and others use liquid nitrogen isopentane. I have heard that LN2 is faster with less ice crystal formation and I think this is a better way to freeze but I need to convince the dry ice people to use LN2. Does anyone have papers that will support my fight for one standard way of freezing. Any thoughts or help with literature would be great. We freezing mouse and rat tissues for IHC and LCM (laser capture microdissection ). Thanks _______________________________ Jamie Erickson Sr. Research Associate Department: DSMP Abbott Bioresearch Center 100 Research Drive Worcester, MA 01605-4341 508-688-3134 FAX: 508-793-4895 e-mail: jamie.erickson@abbott.com From gcallis <@t> montana.edu Thu May 11 10:12:29 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu May 11 10:12:36 2006 Subject: [Histonet] safranin o staining- glycosaminoglycans-cartilage In-Reply-To: References: Message-ID: <6.0.0.22.1.20060511090752.01b1a650@gemini.msu.montana.edu> I will be attaching the safranin O/fast green method to you privately, the original publication was by Rosenberg, reference will be in protocol. Rosenberg originally did this on frozen sections of articular cartilage and it has since been modified for formalin fixed, decalcified bone paraffin sections. It is wise to include an undecalcified, normal piece of articular cartilage as a normal control to see the tinctorial quality compared to a decalcified cartilage to ensure decalcifiers have not affected the glycoaminoglycans. At 05:22 AM 5/11/2006, you wrote: >Dear Histonetters, > I am in need of the procedure for staining of >glycosaminoglycans in articular cartilage. The literature search indicated >the use of safranin o staining for the characterization of >glycosaminoglycans. If any body have good experience in this procedure and >able to share their protocol. I have already ordered a paper that deals >with this. >Method of Histomorphometric Assessment of Glycosaminoglycans in Articular >Cartilage >Journal of Orthopaedic Research Vol 15, 670-674. 1977 >Choji Shimizu, Richard D. Coutts, Robert M. Healey Toshikazu Kubo, Yasusuke >Hirasawa and David Amiel. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From hymclab <@t> hyhc.com Thu May 11 10:19:10 2006 From: hymclab <@t> hyhc.com (hymclab) Date: Thu May 11 10:13:39 2006 Subject: [Histonet] Marking prostate biopsies Message-ID: We put hematoxylin on our cores at the grossing station. We pull out the cores, place them on a towel and use a pipette to place a drop of hematoxylin on each core. The cores are them placed between blue sponges that have been soaked in formalin and them processed. The cores are a nice purple and show up well while embedding and while cutting and of course doesn't interfere with staining. Hope this helps, Dawn -----Original Message----- From: Vickroy, Jim [mailto:Vickroy.Jim@mhsil.com] Sent: Thursday, May 11, 2006 9:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Marking prostate biopsies We are looking for a way to better mark our small needle biopsies such as breast cores and prostate biopsies so that they are more easily seen after processing. Right now the embedder has some trouble identifying the cores and in turn the histotech often has problems seeing the cores in the paraffin block. We are doing levels on these cases but find that often we need recuts because we are not into the block enough. I have heard of some techs who have used a little eosin in their 70% ETOH, which helped the techs identify the cores. I am reluctant to use eosin on my automated processors in the 70% ETOH however. We tried dipping the blocks in eosin tinted formalin at the grossing stations. The eosin leached out and did no good. Does anyone have any good ideas? I do know that some send eosin tinted formalin to sites that are doing these biopsies. I don't see that as an option for us. Thanks for your help. James R. Vickroy Supervisor - MMC Surgical Pathology 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Thu May 11 10:14:27 2006 From: jkiernan <@t> uwo.ca (John A. Kiernan) Date: Thu May 11 10:14:17 2006 Subject: [Histonet] Re: Golgi stain question/Rapid Golgi Stain (FD Neurotech) References: <1706.128.250.186.193.1147241410.squirrel@webmail.unimelb.edu.au> Message-ID: <446354D3.D15CBAB3@uwo.ca> The first thing you need to find out is whst's in the kit. Is the method the one traditionally known as the rapid Golgi method? This involves fixation in an osmium tetroxide-potassium dichromate mixture, for 2 to 7 days (the time affects the outcome, but unpredictably) followed by immersion in aqueous silver nitrate for a few days, and preparing thick celloidin or paraffin sections. There are plenty of later variants, having in common a silver chromate end product, but these should not be called "the rapid Golgi method". The sections are mounted in thick Canada balsam (the real stuff) without coverslips. If a coverslip is applied, the preparations fade with time. There are various chemical tricks to prevent such fading. The other major member of this group of methods is Cox's, with mercuric chloride and potassium dichromate, but no silver. The dark deposit in this case is thought to be a mixture of mercurous oxide and colloidal mercury. These methods are often called Golgi-Cox, even though Golgi didn't invent or use them. Ideally Golgi-Cox preparations should also be mounted without coverslips, but they will keep for a few years in DPX with coverslips. John Kiernan Anatomy, UWO London, Canada ____________________ John Fernandez wrote: > > Hi all, > > our lab has recently purchased and used the Rapid Golgi Stain Kit from FD > Neurotech, resulting in very impressive staining down to dendritic spine > level, however as Julie Heinrich found, within 24 hours this staining > becomes very granular with loss of distinct morphology that was otherwise > seen previously. It progressively worsens over time. > > Has anyone successfully used the FD Neurotech Rapid Golgi kit? Or does > anyone know of a mounting medium that can be used to adequately stop the > reaction that is taking place? Any ideas on what could be causing this > progressive deterioration of staining would be very much appreciated. > > Thanks in advance, > > John > > John Fernandez > Research Assistant > Department of Medicine > University of Melbourne > Austin & Repatriation Medical Centre > Heidelberg, VIC 3084 > Ph: (03) 9496 3257 > > Re: Golgi stain question > From: "J. A. Kiernan" > > -------------------------------------------------------------------------------- > > On Fri, 30 Mar 2001, Julie Heinrich wrote: > > > I am terribly sorry to bother you- I have been looking through the > > histonet web page and have found several helpful replies from you > > about the Gibb & Kolb Golgi stain method. I haven't managed to > > figure out how to properly post a question there - > > You can't. It's a collection of old (archived) communications. > To ask or answer questions you have to subscribe to the > listserver. This is done by sending an email to > histonet@pathology.swmed.edu with the one word subscribe > in the Subject line. Nothing else. You'll then get an automatic > reply from the listserver telling you all about it. > > > I'm attempting to use the method on avian tissue. I have obtained > > some decent looking tissue so far (though the stain is a dark > > tan/brown, rather than the preferable black that I had expected) yet > > after time the stain turns very 'grainy'. Within a matter of just 24 > > hours, the dendrites/spines look like collections of dots rather than > > complete structures, and it gets worse with time. > > > Do you have any idea why this might be happening? (I'm following > > their protocol to the best of my knowledge, and use fresh solutions > > each time I run tissue) > > A graduate student here called Tim Ho did great numbers of Kolb > Golgis on rat brains a few years ago. He went to Kolb's lab in > Lethbridge, Alta for guidance. His initial problem was that the > unstained spaces between the black cells were pale green and > a bit granular. If I remember rightly, the washing after the > Golgi-Cox solution needed to be more thorough. > > Your problem is different, and may relate to the mounting medium. > Traditionally the sections were mounted in thick Canada balsam > without coverslips. A modern synthetic medium + coverslip can > be followed by fading, but I haven't heard of this happening > in 24 hours. 6 months, yes. Are you cutting vibratome sections > of adequate thickness? I think you must be doing something wrong > at or after the sectioning step, but don't know what. Sorry I > can't be more helpful. > > ---------------------------------------- > John A. Kiernan > Department of Anatomy & Cell Biology > The University of Western Ontario > London, Canada N6A 5C1 > kiernan@uwo.ca > http://publish.uwo.ca/~jkiernan > > -------------------------------------------------------------------------------- > > << Previous Message | Next Message >> > -- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dmccaig <@t> ckha.on.ca Thu May 11 10:13:18 2006 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Thu May 11 10:16:59 2006 Subject: [Histonet] STAFF EVALUATIONS Message-ID: Does anyone do an annual evaluation of staff ability to perform certain tasks? Diana McCaig 519-352-6401 (6604) From candy.a.bales <@t> uth.tmc.edu Thu May 11 10:22:25 2006 From: candy.a.bales <@t> uth.tmc.edu (Bales, Candy A) Date: Thu May 11 10:22:33 2006 Subject: [Histonet] Immuno. for Cat Scratch Message-ID: Biocare Medical sells one for Cat Scratch (Bartonella henselae). Source: Mouse. Have not tried products with this company yet, just met local rep. & obtained a catalog. Their phone # 800-799-9499 or www.biocare.net C. Bales UT-Houston -----Original Message----- > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Nancy.Temple <@t> ssfhs.org Thu May 11 10:27:58 2006 From: Nancy.Temple <@t> ssfhs.org (Temple Nancy) Date: Thu May 11 10:28:08 2006 Subject: [Histonet] Marking prostate biopsies Message-ID: We also put Eosin in our last 100% alcohol on the processor. Helps with embedding and especially with cutting of needle bx and other small bx. Our bx are between blue sponges(I like the sponges from SurgiPath best)that have been soaked in formalin. Nancy St. Francis Hospital -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Thursday, May 11, 2006 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Marking prostate biopsies We are looking for a way to better mark our small needle biopsies such as breast cores and prostate biopsies so that they are more easily seen after processing. Right now the embedder has some trouble identifying the cores and in turn the histotech often has problems seeing the cores in the paraffin block. We are doing levels on these cases but find that often we need recuts because we are not into the block enough. I have heard of some techs who have used a little eosin in their 70% ETOH, which helped the techs identify the cores. I am reluctant to use eosin on my automated processors in the 70% ETOH however. We tried dipping the blocks in eosin tinted formalin at the grossing stations. The eosin leached out and did no good. Does anyone have any good ideas? I do know that some send eosin tinted formalin to sites that are doing these biopsies. I don't see that as an option for us. Thanks for your help. James R. Vickroy Supervisor - MMC Surgical Pathology 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu May 11 10:44:03 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 11 10:44:08 2006 Subject: [Histonet] STAFF EVALUATIONS In-Reply-To: Message-ID: <20060511154403.18424.qmail@web61219.mail.yahoo.com> Once a year to all staff member and tasks. During probation there were monthly evaluations. Ren? J. Diana McCaig wrote: Does anyone do an annual evaluation of staff ability to perform certain tasks? Diana McCaig 519-352-6401 (6604) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Love cheap thrills? Enjoy PC-to-Phone calls to 30+ countries for just 2?/min with Yahoo! Messenger with Voice. From gcallis <@t> montana.edu Thu May 11 10:56:47 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu May 11 10:57:08 2006 Subject: [Histonet] Cryopreservation dry ice vs LN2 isopentane In-Reply-To: References: Message-ID: <6.0.0.22.1.20060511094620.01b7e320@gemini.msu.montana.edu> Jamie, Using dry ice mixed with isopentane or hexane will work just as well for snap freezing (i.e. cryopreservation?) as liquid nitrogen/isopentane. The problem with liquid nitrogen and isopentane is the constant recooling of the isopentane to the correct temperature when snap freezing a large number of tissues during a long freezing session We gave up on this method years ago and opted for dry ice/isopentane or hexane slurry. You can also use a petri dish floating in liquid nitrogen, that way you can snap freeze multiple samples rapidly, and get rid of isopentane, always a storage dilemma unless you have explosion proof freezers/refrigerators. We often snap freeze 30 blocks during a dissection/snap freezing session. We also had our OCT crack in the liq N2/isopentane method, it was just too cold. I will be happy to send you a powerpoint showing how this is done (both methods). We do not have freezing artifact. For LCM, a colleague and expert at doing this, snap freezes tissues in dry ice cooled hexane but does NOT put the dry ice into the solvent, she merely surrounds the beaker of hexane with dry ice until correct temperature is needed. You can discuss this with her via email privately, I will send her email address to you privately if you want it. She does work with rodent tissues. No papers, just experience and learning the hard way for our laboratory. MyNeuroLab website has a discussion or at least Charles Scouten on the theory of snap freezing. At 09:03 AM 5/11/2006, you wrote: >Hello histonetters, > I am hoping that someone out there >can help me with a cryopreservation dilemma. I am in a research pathology >lab and we have two schools of cryopreservation here, some researchers use >dry ice isopentane and others use liquid nitrogen isopentane. I have heard >that LN2 is faster with less ice crystal formation and I think this is a >better way to freeze but I need to convince the dry ice people to use LN2. >Does anyone have papers that will support my fight for one standard way of >freezing. Any thoughts or help with literature would be great. We freezing >mouse and rat tissues for IHC and LCM (laser capture microdissection ). > >Thanks > > >_______________________________ >Jamie Erickson >Sr. Research Associate >Department: DSMP >Abbott Bioresearch Center >100 Research Drive >Worcester, MA 01605-4341 >508-688-3134 >FAX: 508-793-4895 >e-mail: jamie.erickson@abbott.com > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From nakagawa <@t> umn.edu Thu May 11 11:12:51 2006 From: nakagawa <@t> umn.edu (yasushi nakagawa) Date: Thu May 11 11:12:58 2006 Subject: [Histonet] autofluorescence? Message-ID: Hi, We are doing immunohistochemistry using Cy2, Cy3 and Cy5-conjugated secondary antibodies on 20um-thick cryosections of embryonic mouse brains. Especially with Cy2- filter setting, we sometimes see a number of auto-fluorescence-like signals within the brain sections. They are thin and long, kind of looking like blood vessels. They show up even when we do not use Cy2-conjugated secondaries. The same problem has happened to Cy3-filter setting, too, although less frequently. I wonder if anyone has a idea about what they are and how to prevent them from showing up. We fix brains in 4%PFA/PB overnight, wash and cryoprotect in sucrose/ PB, and freeze in OCT in plastic molds on petri dish on liquid nitrogen. We cut 20um cryosections, dry them for an hour or so, and do regular antibody staining, do DAPI staining, dehydrate, and mount in DPX. Thank you for your help. Yasushi Nakagawa From lrichey <@t> u.washington.edu Thu May 11 11:20:03 2006 From: lrichey <@t> u.washington.edu (Lori Richey) Date: Thu May 11 11:20:18 2006 Subject: [Histonet] Marking prostate biopsies In-Reply-To: References: Message-ID: <44636433.8040800@u.washington.edu> Mercurichrome works well for cardiac bxs. Not sure what percent. Vickroy, Jim wrote: > > >We are looking for a way to better mark our small needle biopsies such >as breast cores and prostate biopsies so that they are more easily seen >after processing. > >Right now the embedder has some trouble identifying the cores and in >turn the histotech often has problems seeing the cores in the paraffin >block. > >We are doing levels on these cases but find that often we need recuts >because we are not into the block enough. > > > >I have heard of some techs who have used a little eosin in their 70% >ETOH, which helped the techs identify the cores. I am reluctant to use >eosin on my automated processors in the 70% ETOH however. We tried >dipping the blocks in eosin tinted formalin at the grossing stations. >The eosin leached out and did no good. Does anyone have any good >ideas? > > > >I do know that some send eosin tinted formalin to sites that are doing >these biopsies. I don't see that as an option for us. Thanks for your >help. > > > >James R. Vickroy > >Supervisor - MMC Surgical Pathology > >217-788-4046 > > > > > >This message (including any attachments) contains confidential information intended for a >specific individual and purpose, and is protected by law. If you are not the intended recipient, >you should delete this message. Any disclosure, copying, or distribution of this message, or the >taking of any action based on it, is strictly prohibited. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From jcline <@t> wchsys.org Thu May 11 12:03:06 2006 From: jcline <@t> wchsys.org (Joyce Cline) Date: Thu May 11 12:03:28 2006 Subject: [Histonet] Marking prostate biopsies In-Reply-To: Message-ID: <000001c6751c$c702c760$1d2a14ac@wchsys.org> We use Toluidine Blue 0.5% alcoholic to mark all our prostate, GI's, and cervical biopsies. The blue color stays through processing. ....................................................................... We are looking for a way to better mark our small needle biopsies such as breast cores and prostate biopsies so that they are more easily seen after processing. ETC. Vickroy Supervisor - MMC Surgical Pathology ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From SHARON.OSBORN <@t> SPCORP.COM Thu May 11 12:02:51 2006 From: SHARON.OSBORN <@t> SPCORP.COM (Osborn, Sharon) Date: Thu May 11 12:03:56 2006 Subject: [Histonet] RE: Slide labelers Message-ID: <9A919A5D70313A4D9C56A025710874080C728C@kenmsg40.us.schp.com> Kim, Experience tells me that your work will increase once you begin doing any type of automation. We originally had the TBS slide and cassette labelers and they were work horses; however time consuming because of the fine powder generated by etching the slides which we had to spend lots of time and water washing off. Note: Thermo-Shandon is based on the same principle. The TBS served a purpose for they were the first major automation for such labeling. I have also used the Surgipath and prefer a different system because of the way Surgipath has you input the data. Now, we have the SECOND generation of the Leica Slide printer and our usage of t has tripled over what was used on the TBS. Other departments come to label their slides plus we now label over 90% of the IHC slides on the printer where due to the dust, etc, these were done by hand before. I want to state here that the first Leica slide printer was such an improvement over the TBS type system that we felt we were in heaven. However, due to customer suggestions and some minor irritating problems, Leica took all these comments to heart and re-engineered the insides of the printers. We have received this new slide printer---it is FANTASTIC! It is quieter yes, really) than the first one, operates more smoothly and keeps purring right along. Now, we are waiting with baited breath for the 2nd generation cassette labeler (Leica,are you listening?) Since this is a major investment anyway, I suggest that you contact Leica and have them bring them out to demo. Whatever system you get, please let me know if the labeling usage increases over your hand written slides and cassettes. Automation for labeling certainly decreases the repetitive motion problems that we can develop. Sharon Osborn DNAX, ScheringPlough BioPharma Palo Alto, CA 650.496.6539 Date: Wed, 10 May 2006 10:07:27 -0700 From: "Kimberly Johnson" Subject: [Histonet] Slide Labeler To: Message-ID: <96DACB13ECACA645A47020B3E136776C0FA054@covxmail.covx.com> Content-Type: text/plain; charset="us-ascii" Hello Histonet. First let me say, long time reader, first time poster, so this is very exciting! My small biotech company has decided it is time for me to get a slide labeler! I would love some input as to what you all think works well, keep in mind this is a small company doing very simple and not much IHC. Thanks for your time! Kim ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From Dorothy.L.Webb <@t> HealthPartners.Com Thu May 11 12:20:33 2006 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Thu May 11 12:20:40 2006 Subject: [Histonet] gluteraldehyde Message-ID: <0E394B648E5284478A6CCB78E5AFDA270171FDB5@hpes1.HealthPartners.int> How is everyone disposing if gluteraldehyde in a 3% solution? We obtain ours from Mayo and have some that is outdated, but, they weren't very informative in telling me how to dispose of it. Thanks histonetters for your advice!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From Julie.Sanders <@t> va.gov Thu May 11 12:23:20 2006 From: Julie.Sanders <@t> va.gov (Sanders, Julie, VHACIN) Date: Thu May 11 12:23:27 2006 Subject: [Histonet] RE: Histonet Digest, Vol 30, Issue 15 Message-ID: <2D4ACE41DEFE93428F23D77988EFBCB50E5FC3@VHAV10MSGA2.v10.med.va.gov> Diana McCaig wrote: Does anyone do an annual evaluation of staff ability to perform certain tasks? Diana McCaig 519-352-6401 (6604) We do yearly competency evaluations which includes all tasks required for histology techs, continuing ed and things like fire, safety, etc. Julie Sanders, BA, HT(ASCP) Supervisor, Anatomic Pathology VAMC Cincinnati From sobrien <@t> bthosp.com Thu May 11 11:51:33 2006 From: sobrien <@t> bthosp.com (O'Brien, Sue) Date: Thu May 11 12:26:29 2006 Subject: [Histonet] Formalin vs. Saline Specimen Submission Message-ID: <4D95C24EC0E4C84787A6919F1165B25E9CB34E@btmhems01.BTHOSP.INT> I was wondering how other hospitals handled specimens submitted from the OR (Operating Room). Currently, our routine specimens (ones that do not require special testing and submission) are submitted in 10% NBF (Neutral Buffered Formalin). Thank-you for taking the time to respond! How are routine specimens submitted to your lab from the OR (chose one)? a. in formalin (e.g. 10% NBF) b. in saline c. fresh d. other (specify): A. For specimens are received by OR in formalin: Is the specimen placed in formalin in the OR procedure room? If yes, how is it done? a. under a fume hood b. placed in container which has formalin c. other (specify): If no, how is it done? a. specimen placed in container and taken fresh to another room where formalin is added b. other (specify): Does the OR have a fume hood available for use (to put specimens into formalin under it)? B. For specimens submitted by OR in saline: a. How long (generally) is the specimen in saline until it is placed into formalin? b. What volume of saline is used? (e.g. enough to keep moist, or enough to submerge specimen)? c. Who transfers the specimen into formalin? (e.g. histo or lab tech at time of receipt, or does it wait until the Pathologist grosses it? If it waits for the Pathologist, then for how long, and how is it refrigerated?) d. Are specimens refrigerated? If yes, where (e.g. in OR, in lab, in histology). C. For specimens submitted fresh: a. how long (generally) is the specimen fresh before placement into formalin? b. How do you keep the small specimens from drying out? c. Who transfers the specimen into formalin? (e.g. histo or tech at time of receipt, or does it wait until the Pathologist grosses it? If it waits for the Pathologist, then for how long, and how is it refrigerated?) d. Are specimens refrigerated? If yes, where (e.g. in OR, in lab, in histology). If you receive specimens either in saline or fresh (and they are not refrigerated immediately): a. How do you feel they compare to specimens that were immediately placed into formalin? (e.g. better morphology, worse morphology, etc). b. Who handles specimens submitted after hours? (e.g. weekends/holidays; are they left at room temp, refrigerated, or does someone (who?) transfer them into formalin (how?). Sorry for the length, but I would really appreciate knowing how others handle this. Sincerely, Susan O'Brien, Histology Supervisor Burdette Tomlin Memorial Hospital Cape May Court House, NJ 08210 From DOOLEEO <@t> shands.ufl.edu Thu May 11 12:07:44 2006 From: DOOLEEO <@t> shands.ufl.edu (Elaine Dooley) Date: Thu May 11 12:26:32 2006 Subject: [Histonet] MGMT antibody Message-ID: Dear Histonetters, I have been trying to get MGMT antibody to work on a Ventana Benchmark immunostainer and I am not getting the good results yet. The MGMT (clone MT 3.1 from Novus Biologicals) supposed to stain Colon carcinoma. So I tried 1:100 with CC1 antigen retrieval and did not get any staining. I tried 1:20 with amplify and CC1 retrieval and everything was staining. So I tried 1:40 with and without amplifier, both with CC1 retrieval and they stained everything weakly. Perhaps a different retrieval should be used. Or maybe it does not stain all colon carcinomas and I am trying to make one stain that really should not. I have limited time and thought if anyone might have some insight I would appreciate it. Elaine Dooley HTL Shands labs at Rocky Point 352-265-0111 ext 72117 From lucyb <@t> biocare.net Thu May 11 12:31:02 2006 From: lucyb <@t> biocare.net (Lucy Brooks) Date: Thu May 11 12:31:03 2006 Subject: [Histonet] Immuno. for Cat Scratch Message-ID: <000001c67520$ad29d140$6e00640a@biocaredomain.com> Mirian, Biocare Medical has a cat scratch antibody Part # CM144C, or PM144AA, Please feel free to email me back at Biocare if you would like more information on it. Lucy Brooks Technical Sales Representative Biocare Medical LLC (925) 603-8020 This Email and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. This communication may contain confidential material protected by the physician-patient privilege. If you are not the intended recipient or the person responsible for delivering the Email to the intended recipient, be advised that you have received the Email in error and that any unauthorized use, dissemination, forwarding, printing, or copying of the Email is strictly prohibited by state and federal laws. If you have received this Email in error, please immediately notify sender by reply Email. From SAllen <@t> exchange.hsc.mb.ca Thu May 11 12:33:10 2006 From: SAllen <@t> exchange.hsc.mb.ca (Sharon Allen) Date: Thu May 11 12:34:44 2006 Subject: [Histonet] Xylol Substitute - Thanks Message-ID: <36FE435D6D2F1D489174B22362A961B66B8339@hscxntmx0006.hsc.mb.ca> Thanks to everyone that sent me a response about the xylol substitute, it will help to solve my problem. Sharon This e-mail and/or any documents in this transmission is intended for the address(s) only and may contain legally privileged or confidential information. Any unauthorized use, disclosure, distribution, copying or dissemination is strictly prohibited. If you receive this transmission in error, please notify the sender immediately and return the original. From Michael.Rice <@t> holy-cross.com Thu May 11 12:46:24 2006 From: Michael.Rice <@t> holy-cross.com (Rice, Michael) Date: Thu May 11 12:46:36 2006 Subject: [Histonet] RE: Histonet Digest, Vol 30, Issue 15 Message-ID: <3BC92F29BE821745AB15E04C98EE028D020FC919@HCH2KMAIL.holy-cross.com> We have been using eosin in the 95% station on our processors for years and have never had a problem. It is a life server for all of the smaller and smaller biopsies that we are receiving these days in addition to dealing with older techs like me with failing eyesight. It is the perfect storm. Mike Michael Rice CT.HT(ASCP) Supervisor Of Pathology Holy Cross Hospital Ft Lauderdale, Fl 33308 954.776.3070 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, May 11, 2006 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 30, Issue 15 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: double immunostaining (Johnson, Teri) 2. Marking prostate biopsies (Vickroy, Jim) 3. Anatech Hp Stain (Parker, Helayne) 4. RE: Marking prostate biopsies (Kemlo Rogerson) 5. RE: Marking prostate biopsies (Gladney, Diane C Ms MACH) 6. RE: Immuno. for Cat Scratch (Monfils, Paul) 7. Re: Marking prostate biopsies (Fred Underwood) 8. Cryopreservation dry ice vs LN2 isopentane (Jamie E Erickson) 9. Re: safranin o staining- glycosaminoglycans-cartilage (Gayle Callis) 10. RE: Marking prostate biopsies (hymclab) 11. Re: Re: Golgi stain question/Rapid Golgi Stain (FD Neurotech) (John A. Kiernan) 12. STAFF EVALUATIONS (Diana McCaig) 13. RE: Immuno. for Cat Scratch (Bales, Candy A) 14. RE: Marking prostate biopsies (Temple Nancy) 15. Re: STAFF EVALUATIONS (Rene J Buesa) 16. Re: Cryopreservation dry ice vs LN2 isopentane (Gayle Callis) 17. autofluorescence? (yasushi nakagawa) 18. Re: Marking prostate biopsies (Lori Richey) ---------------------------------------------------------------------- Message: 1 Date: Thu, 11 May 2006 09:17:12 -0500 From: "Johnson, Teri" Subject: [Histonet] Re: double immunostaining To: Message-ID: Content-Type: text/plain; charset="us-ascii" Dear Richard, Kits are not needed to design and successfully do doublestaining. I recommend you buy Chris van der Loos' book on the subject, and therein lies pretty much all the information you will need. ISBN: 038791594X You should be able to get this through Amazon.com and other online book vendors. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64133 ------------------------------ Message: 2 Date: Thu, 11 May 2006 09:28:50 -0500 From: "Vickroy, Jim" Subject: [Histonet] Marking prostate biopsies To: Message-ID: Content-Type: text/plain; charset="us-ascii" We are looking for a way to better mark our small needle biopsies such as breast cores and prostate biopsies so that they are more easily seen after processing. Right now the embedder has some trouble identifying the cores and in turn the histotech often has problems seeing the cores in the paraffin block. We are doing levels on these cases but find that often we need recuts because we are not into the block enough. I have heard of some techs who have used a little eosin in their 70% ETOH, which helped the techs identify the cores. I am reluctant to use eosin on my automated processors in the 70% ETOH however. We tried dipping the blocks in eosin tinted formalin at the grossing stations. The eosin leached out and did no good. Does anyone have any good ideas? I do know that some send eosin tinted formalin to sites that are doing these biopsies. I don't see that as an option for us. Thanks for your help. James R. Vickroy Supervisor - MMC Surgical Pathology 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ------------------------------ Message: 3 Date: Thu, 11 May 2006 09:31:59 -0500 From: "Parker, Helayne" Subject: [Histonet] Anatech Hp Stain To: Message-ID: <2FABC6145388F24EBA8CBD6EC165BCCB02AFBA69@mail1-schc.skaggs.net> Content-Type: text/plain; charset="US-ASCII" Hi All, We have been having trouble with the Hp stain. We get green instead of bright yellow. Depending on the green we can easily see the bacteria and other times not. Dr. is complaining as very inconsistent. We have tried the product insert suggestion to no great avail. Any suggestions, Helayne Parker, HT (A.S.C.P.) Histology Section Head Skaggs Community Health Center Branson, Missouri ------------------------------ Message: 4 Date: Thu, 11 May 2006 15:36:35 +0100 From: Kemlo Rogerson Subject: RE: [Histonet] Marking prostate biopsies To: "'Vickroy, Jim'" , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain I used to dip breast biopsy cores into very dilute alcoholic eosin just for a few seconds and they came through the Processors OK. You could post fix after formalin with a picric acid fixative but then you'd have to wash it out. If you decide to use eosin then don't use a single receptacle for dipping the biopsies into; cross over. I used to put a few drops into the upturned lid of the biopsy pot and then dip the cores in, one by one, then put into the cassette. I used then to forget and put the lid on the pot and cover myself in eosin, and the floor, and my coat, and the MLA next to me. But better that then give someone a false positive result. A bit red in the face..... Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 The real does not die, the unreal never lived. --Nisargadatta Maharaj This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation ------------------------------ Message: 5 Date: Thu, 11 May 2006 10:36:02 -0400 From: "Gladney, Diane C Ms MACH" Subject: RE: [Histonet] Marking prostate biopsies To: "Vickroy, Jim" , Message-ID: <4D55B2E997EFAE4DA6081DDE100B8302DE91FD@amedmlsermc133> Content-Type: text/plain; charset="us-ascii" I use Eosin in my last 100% Ethanol on my tissue processor. It works great for the small biopsies of other tissue also. We have not had any problems seeing these small biopsies or needle core biopsies since I started doing this. Diane Diane C. Gladney, HT (ASCP) Supervisor, Histology Dept. Moncrief Army Community Hospital Dept. of Pathology 4500 Stuart St. FT. Jackson, SC 29207 Email: diane.gladney@se.amedd.army.mil Phone: 803-751-2530 FAX: 803-751-7829 DSN: 734-2530 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Thursday, May 11, 2006 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Marking prostate biopsies We are looking for a way to better mark our small needle biopsies such as breast cores and prostate biopsies so that they are more easily seen after processing. Right now the embedder has some trouble identifying the cores and in turn the histotech often has problems seeing the cores in the paraffin block. We are doing levels on these cases but find that often we need recuts because we are not into the block enough. I have heard of some techs who have used a little eosin in their 70% ETOH, which helped the techs identify the cores. I am reluctant to use eosin on my automated processors in the 70% ETOH however. We tried dipping the blocks in eosin tinted formalin at the grossing stations. The eosin leached out and did no good. Does anyone have any good ideas? I do know that some send eosin tinted formalin to sites that are doing these biopsies. I don't see that as an option for us. Thanks for your help. James R. Vickroy Supervisor - MMC Surgical Pathology 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Thu, 11 May 2006 10:41:16 -0400 From: "Monfils, Paul" Subject: RE: [Histonet] Immuno. for Cat Scratch To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <09C945920A6B654199F7A58A1D7D1FDE017176F7@lsexch.lsmaster.lifespan.org> Content-Type: text/plain; charset="iso-8859-1" Antibody against Bartonella henselae, the agent of cat scratch disease, exists, because some commercial labs offer the immunofluorescence technique. However, I don't know of a source for the antibody. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > Marian Powers > Sent: Wednesday, May 10, 2006 5:47 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Immuno. for Cat Scratch > > Wondering if there is an antibody for Cat Scratch Bacillus? > > Thanks in advance, > > Marian > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 7 Date: Thu, 11 May 2006 10:51:29 -0400 From: "Fred Underwood" Subject: Re: [Histonet] Marking prostate biopsies To: , Message-ID: Content-Type: text/plain; charset=US-ASCII I have used Mrs. Stewarts Bluing Agent. It's a product used for whitening clothes. We would dip prostate biopsies, or place a drop onto the biopsy, at the grossing table. Blot off any excess,wrap in lens paper, and process. The biopsies would be colored a highly visible blue. Fred >>> "Vickroy, Jim" 05/11/06 10:28AM >>> We are looking for a way to better mark our small needle biopsies such as breast cores and prostate biopsies so that they are more easily seen after processing. Right now the embedder has some trouble identifying the cores and in turn the histotech often has problems seeing the cores in the paraffin block. We are doing levels on these cases but find that often we need recuts because we are not into the block enough. I have heard of some techs who have used a little eosin in their 70% ETOH, which helped the techs identify the cores. I am reluctant to use eosin on my automated processors in the 70% ETOH however. We tried dipping the blocks in eosin tinted formalin at the grossing stations. The eosin leached out and did no good. Does anyone have any good ideas? I do know that some send eosin tinted formalin to sites that are doing these biopsies. I don't see that as an option for us. Thanks for your help. James R. Vickroy Supervisor - MMC Surgical Pathology 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Thu, 11 May 2006 11:03:24 -0400 From: Jamie E Erickson Subject: [Histonet] Cryopreservation dry ice vs LN2 isopentane To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Hello histonetters, I am hoping that someone out there can help me with a cryopreservation dilemma. I am in a research pathology lab and we have two schools of cryopreservation here, some researchers use dry ice isopentane and others use liquid nitrogen isopentane. I have heard that LN2 is faster with less ice crystal formation and I think this is a better way to freeze but I need to convince the dry ice people to use LN2. Does anyone have papers that will support my fight for one standard way of freezing. Any thoughts or help with literature would be great. We freezing mouse and rat tissues for IHC and LCM (laser capture microdissection ). Thanks _______________________________ Jamie Erickson Sr. Research Associate Department: DSMP Abbott Bioresearch Center 100 Research Drive Worcester, MA 01605-4341 508-688-3134 FAX: 508-793-4895 e-mail: jamie.erickson@abbott.com ------------------------------ Message: 9 Date: Thu, 11 May 2006 09:12:29 -0600 From: Gayle Callis Subject: Re: [Histonet] safranin o staining- glycosaminoglycans-cartilage To: PKamalavenkatesh@wockhardtin.com, Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20060511090752.01b1a650@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed I will be attaching the safranin O/fast green method to you privately, the original publication was by Rosenberg, reference will be in protocol. Rosenberg originally did this on frozen sections of articular cartilage and it has since been modified for formalin fixed, decalcified bone paraffin sections. It is wise to include an undecalcified, normal piece of articular cartilage as a normal control to see the tinctorial quality compared to a decalcified cartilage to ensure decalcifiers have not affected the glycoaminoglycans. At 05:22 AM 5/11/2006, you wrote: >Dear Histonetters, > I am in need of the procedure for staining of >glycosaminoglycans in articular cartilage. The literature search indicated >the use of safranin o staining for the characterization of >glycosaminoglycans. If any body have good experience in this procedure and >able to share their protocol. I have already ordered a paper that deals >with this. >Method of Histomorphometric Assessment of Glycosaminoglycans in Articular >Cartilage >Journal of Orthopaedic Research Vol 15, 670-674. 1977 >Choji Shimizu, Richard D. Coutts, Robert M. Healey Toshikazu Kubo, Yasusuke >Hirasawa and David Amiel. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) ------------------------------ Message: 10 Date: Thu, 11 May 2006 10:19:10 -0500 From: hymclab Subject: RE: [Histonet] Marking prostate biopsies To: "'Vickroy, Jim'" , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain We put hematoxylin on our cores at the grossing station. We pull out the cores, place them on a towel and use a pipette to place a drop of hematoxylin on each core. The cores are them placed between blue sponges that have been soaked in formalin and them processed. The cores are a nice purple and show up well while embedding and while cutting and of course doesn't interfere with staining. Hope this helps, Dawn -----Original Message----- From: Vickroy, Jim [mailto:Vickroy.Jim@mhsil.com] Sent: Thursday, May 11, 2006 9:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Marking prostate biopsies We are looking for a way to better mark our small needle biopsies such as breast cores and prostate biopsies so that they are more easily seen after processing. Right now the embedder has some trouble identifying the cores and in turn the histotech often has problems seeing the cores in the paraffin block. We are doing levels on these cases but find that often we need recuts because we are not into the block enough. I have heard of some techs who have used a little eosin in their 70% ETOH, which helped the techs identify the cores. I am reluctant to use eosin on my automated processors in the 70% ETOH however. We tried dipping the blocks in eosin tinted formalin at the grossing stations. The eosin leached out and did no good. Does anyone have any good ideas? I do know that some send eosin tinted formalin to sites that are doing these biopsies. I don't see that as an option for us. Thanks for your help. James R. Vickroy Supervisor - MMC Surgical Pathology 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Thu, 11 May 2006 11:14:27 -0400 From: "John A. Kiernan" Subject: Re: [Histonet] Re: Golgi stain question/Rapid Golgi Stain (FD Neurotech) To: John Fernandez Cc: histonet@lists.utsouthwestern.edu Message-ID: <446354D3.D15CBAB3@uwo.ca> Content-Type: text/plain; charset=us-ascii The first thing you need to find out is whst's in the kit. Is the method the one traditionally known as the rapid Golgi method? This involves fixation in an osmium tetroxide-potassium dichromate mixture, for 2 to 7 days (the time affects the outcome, but unpredictably) followed by immersion in aqueous silver nitrate for a few days, and preparing thick celloidin or paraffin sections. There are plenty of later variants, having in common a silver chromate end product, but these should not be called "the rapid Golgi method". The sections are mounted in thick Canada balsam (the real stuff) without coverslips. If a coverslip is applied, the preparations fade with time. There are various chemical tricks to prevent such fading. The other major member of this group of methods is Cox's, with mercuric chloride and potassium dichromate, but no silver. The dark deposit in this case is thought to be a mixture of mercurous oxide and colloidal mercury. These methods are often called Golgi-Cox, even though Golgi didn't invent or use them. Ideally Golgi-Cox preparations should also be mounted without coverslips, but they will keep for a few years in DPX with coverslips. John Kiernan Anatomy, UWO London, Canada ____________________ John Fernandez wrote: > > Hi all, > > our lab has recently purchased and used the Rapid Golgi Stain Kit from FD > Neurotech, resulting in very impressive staining down to dendritic spine > level, however as Julie Heinrich found, within 24 hours this staining > becomes very granular with loss of distinct morphology that was otherwise > seen previously. It progressively worsens over time. > > Has anyone successfully used the FD Neurotech Rapid Golgi kit? Or does > anyone know of a mounting medium that can be used to adequately stop the > reaction that is taking place? Any ideas on what could be causing this > progressive deterioration of staining would be very much appreciated. > > Thanks in advance, > > John > > John Fernandez > Research Assistant > Department of Medicine > University of Melbourne > Austin & Repatriation Medical Centre > Heidelberg, VIC 3084 > Ph: (03) 9496 3257 > > Re: Golgi stain question > From: "J. A. Kiernan" > > -------------------------------------------------------------------------------- > > On Fri, 30 Mar 2001, Julie Heinrich wrote: > > > I am terribly sorry to bother you- I have been looking through the > > histonet web page and have found several helpful replies from you > > about the Gibb & Kolb Golgi stain method. I haven't managed to > > figure out how to properly post a question there - > > You can't. It's a collection of old (archived) communications. > To ask or answer questions you have to subscribe to the > listserver. This is done by sending an email to > histonet@pathology.swmed.edu with the one word subscribe > in the Subject line. Nothing else. You'll then get an automatic > reply from the listserver telling you all about it. > > > I'm attempting to use the method on avian tissue. I have obtained > > some decent looking tissue so far (though the stain is a dark > > tan/brown, rather than the preferable black that I had expected) yet > > after time the stain turns very 'grainy'. Within a matter of just 24 > > hours, the dendrites/spines look like collections of dots rather than > > complete structures, and it gets worse with time. > > > Do you have any idea why this might be happening? (I'm following > > their protocol to the best of my knowledge, and use fresh solutions > > each time I run tissue) > > A graduate student here called Tim Ho did great numbers of Kolb > Golgis on rat brains a few years ago. He went to Kolb's lab in > Lethbridge, Alta for guidance. His initial problem was that the > unstained spaces between the black cells were pale green and > a bit granular. If I remember rightly, the washing after the > Golgi-Cox solution needed to be more thorough. > > Your problem is different, and may relate to the mounting medium. > Traditionally the sections were mounted in thick Canada balsam > without coverslips. A modern synthetic medium + coverslip can > be followed by fading, but I haven't heard of this happening > in 24 hours. 6 months, yes. Are you cutting vibratome sections > of adequate thickness? I think you must be doing something wrong > at or after the sectioning step, but don't know what. Sorry I > can't be more helpful. > > ---------------------------------------- > John A. Kiernan > Department of Anatomy & Cell Biology > The University of Western Ontario > London, Canada N6A 5C1 > kiernan@uwo.ca > http://publish.uwo.ca/~jkiernan > > -------------------------------------------------------------------------------- > > << Previous Message | Next Message >> > -- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Thu, 11 May 2006 11:13:18 -0400 From: "Diana McCaig" Subject: [Histonet] STAFF EVALUATIONS To: "Histonet \(E-mail\)" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Does anyone do an annual evaluation of staff ability to perform certain tasks? Diana McCaig 519-352-6401 (6604) ------------------------------ Message: 13 Date: Thu, 11 May 2006 10:22:25 -0500 From: "Bales, Candy A" Subject: RE: [Histonet] Immuno. for Cat Scratch To: Message-ID: Content-Type: text/plain; charset="us-ascii" Biocare Medical sells one for Cat Scratch (Bartonella henselae). Source: Mouse. Have not tried products with this company yet, just met local rep. & obtained a catalog. Their phone # 800-799-9499 or www.biocare.net C. Bales UT-Houston -----Original Message----- > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Thu, 11 May 2006 11:27:58 -0400 From: "Temple Nancy" Subject: RE: [Histonet] Marking prostate biopsies To: Message-ID: Content-Type: text/plain; charset="us-ascii" We also put Eosin in our last 100% alcohol on the processor. Helps with embedding and especially with cutting of needle bx and other small bx. Our bx are between blue sponges(I like the sponges from SurgiPath best)that have been soaked in formalin. Nancy St. Francis Hospital -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Thursday, May 11, 2006 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Marking prostate biopsies We are looking for a way to better mark our small needle biopsies such as breast cores and prostate biopsies so that they are more easily seen after processing. Right now the embedder has some trouble identifying the cores and in turn the histotech often has problems seeing the cores in the paraffin block. We are doing levels on these cases but find that often we need recuts because we are not into the block enough. I have heard of some techs who have used a little eosin in their 70% ETOH, which helped the techs identify the cores. I am reluctant to use eosin on my automated processors in the 70% ETOH however. We tried dipping the blocks in eosin tinted formalin at the grossing stations. The eosin leached out and did no good. Does anyone have any good ideas? I do know that some send eosin tinted formalin to sites that are doing these biopsies. I don't see that as an option for us. Thanks for your help. James R. Vickroy Supervisor - MMC Surgical Pathology 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Thu, 11 May 2006 08:44:03 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] STAFF EVALUATIONS To: Diana McCaig , "Histonet \(E-mail\)" Message-ID: <20060511154403.18424.qmail@web61219.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Once a year to all staff member and tasks. During probation there were monthly evaluations. Ren? J. Diana McCaig wrote: Does anyone do an annual evaluation of staff ability to perform certain tasks? Diana McCaig 519-352-6401 (6604) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Love cheap thrills? Enjoy PC-to-Phone calls to 30+ countries for just 2?/min with Yahoo! Messenger with Voice. ------------------------------ Message: 16 Date: Thu, 11 May 2006 09:56:47 -0600 From: Gayle Callis Subject: Re: [Histonet] Cryopreservation dry ice vs LN2 isopentane To: Jamie E Erickson , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20060511094620.01b7e320@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Jamie, Using dry ice mixed with isopentane or hexane will work just as well for snap freezing (i.e. cryopreservation?) as liquid nitrogen/isopentane. The problem with liquid nitrogen and isopentane is the constant recooling of the isopentane to the correct temperature when snap freezing a large number of tissues during a long freezing session We gave up on this method years ago and opted for dry ice/isopentane or hexane slurry. You can also use a petri dish floating in liquid nitrogen, that way you can snap freeze multiple samples rapidly, and get rid of isopentane, always a storage dilemma unless you have explosion proof freezers/refrigerators. We often snap freeze 30 blocks during a dissection/snap freezing session. We also had our OCT crack in the liq N2/isopentane method, it was just too cold. I will be happy to send you a powerpoint showing how this is done (both methods). We do not have freezing artifact. For LCM, a colleague and expert at doing this, snap freezes tissues in dry ice cooled hexane but does NOT put the dry ice into the solvent, she merely surrounds the beaker of hexane with dry ice until correct temperature is needed. You can discuss this with her via email privately, I will send her email address to you privately if you want it. She does work with rodent tissues. No papers, just experience and learning the hard way for our laboratory. MyNeuroLab website has a discussion or at least Charles Scouten on the theory of snap freezing. At 09:03 AM 5/11/2006, you wrote: >Hello histonetters, > I am hoping that someone out there >can help me with a cryopreservation dilemma. I am in a research pathology >lab and we have two schools of cryopreservation here, some researchers use >dry ice isopentane and others use liquid nitrogen isopentane. I have heard >that LN2 is faster with less ice crystal formation and I think this is a >better way to freeze but I need to convince the dry ice people to use LN2. >Does anyone have papers that will support my fight for one standard way of >freezing. Any thoughts or help with literature would be great. We freezing >mouse and rat tissues for IHC and LCM (laser capture microdissection ). > >Thanks > > >_______________________________ >Jamie Erickson >Sr. Research Associate >Department: DSMP >Abbott Bioresearch Center >100 Research Drive >Worcester, MA 01605-4341 >508-688-3134 >FAX: 508-793-4895 >e-mail: jamie.erickson@abbott.com > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) ------------------------------ Message: 17 Date: Thu, 11 May 2006 11:12:51 -0500 From: yasushi nakagawa Subject: [Histonet] autofluorescence? To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed Hi, We are doing immunohistochemistry using Cy2, Cy3 and Cy5-conjugated secondary antibodies on 20um-thick cryosections of embryonic mouse brains. Especially with Cy2- filter setting, we sometimes see a number of auto-fluorescence-like signals within the brain sections. They are thin and long, kind of looking like blood vessels. They show up even when we do not use Cy2-conjugated secondaries. The same problem has happened to Cy3-filter setting, too, although less frequently. I wonder if anyone has a idea about what they are and how to prevent them from showing up. We fix brains in 4%PFA/PB overnight, wash and cryoprotect in sucrose/ PB, and freeze in OCT in plastic molds on petri dish on liquid nitrogen. We cut 20um cryosections, dry them for an hour or so, and do regular antibody staining, do DAPI staining, dehydrate, and mount in DPX. Thank you for your help. Yasushi Nakagawa ------------------------------ Message: 18 Date: Thu, 11 May 2006 09:20:03 -0700 From: Lori Richey Subject: Re: [Histonet] Marking prostate biopsies To: "Vickroy, Jim" Cc: histonet@lists.utsouthwestern.edu Message-ID: <44636433.8040800@u.washington.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Mercurichrome works well for cardiac bxs. Not sure what percent. Vickroy, Jim wrote: > > >We are looking for a way to better mark our small needle biopsies such >as breast cores and prostate biopsies so that they are more easily seen >after processing. > >Right now the embedder has some trouble identifying the cores and in >turn the histotech often has problems seeing the cores in the paraffin >block. > >We are doing levels on these cases but find that often we need recuts >because we are not into the block enough. > > > >I have heard of some techs who have used a little eosin in their 70% >ETOH, which helped the techs identify the cores. I am reluctant to use >eosin on my automated processors in the 70% ETOH however. We tried >dipping the blocks in eosin tinted formalin at the grossing stations. >The eosin leached out and did no good. Does anyone have any good >ideas? > > > >I do know that some send eosin tinted formalin to sites that are doing >these biopsies. I don't see that as an option for us. Thanks for your >help. > > > >James R. Vickroy > >Supervisor - MMC Surgical Pathology > >217-788-4046 > > > > > >This message (including any attachments) contains confidential information intended for a >specific individual and purpose, and is protected by law. If you are not the intended recipient, >you should delete this message. Any disclosure, copying, or distribution of this message, or the >taking of any action based on it, is strictly prohibited. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 30, Issue 15 **************************************** ====================== Confidentiality Notice: Prepared in Anticipation of Litigation. Attorney-Client Privileged. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original. ====================== From KParker <@t> mail.nih.gov Thu May 11 12:59:19 2006 From: KParker <@t> mail.nih.gov (Tuttle, Kimberly (NIH/NCI) [E]) Date: Thu May 11 12:59:24 2006 Subject: [Histonet] Manual processing In-Reply-To: <3BC92F29BE821745AB15E04C98EE028D020FC919@HCH2KMAIL.holy-cross.com> Message-ID: Does anyone have a written procedure for manual processing of Tissue? Kimberly C. Tuttle H.T.,(ASCP) NCI Center for Cancer Research Tissue Array Research Program http://resresources.nci.nih.gov/tarp From cfavara <@t> niaid.nih.gov Thu May 11 13:03:17 2006 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID) [E]) Date: Thu May 11 13:03:24 2006 Subject: [Histonet] MGMT antibody In-Reply-To: Message-ID: I am not familiar with this antibody but I use the Ventana system with a number of research antibodies and find that I need to boost the Streptavidin often with antibodies not provided by Ventana. You may not be able to do this if you do not have their research software. In that case I would try an overnight incubation. Hope this is helpful - if you would like more specifics about the boost let me know. c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives -----Original Message----- From: Elaine Dooley [mailto:DOOLEEO@shands.ufl.edu] Sent: Thursday, May 11, 2006 10:08 AM To: Histonet@pathology.swmed.edu Subject: [Histonet] MGMT antibody Dear Histonetters, I have been trying to get MGMT antibody to work on a Ventana Benchmark immunostainer and I am not getting the good results yet. The MGMT (clone MT 3.1 from Novus Biologicals) supposed to stain Colon carcinoma. So I tried 1:100 with CC1 antigen retrieval and did not get any staining. I tried 1:20 with amplify and CC1 retrieval and everything was staining. So I tried 1:40 with and without amplifier, both with CC1 retrieval and they stained everything weakly. Perhaps a different retrieval should be used. Or maybe it does not stain all colon carcinomas and I am trying to make one stain that really should not. I have limited time and thought if anyone might have some insight I would appreciate it. Elaine Dooley HTL Shands labs at Rocky Point 352-265-0111 ext 72117 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LuckG <@t> empirehealth.org Thu May 11 13:04:14 2006 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Thu May 11 13:04:17 2006 Subject: [Histonet] Marking prostate biopsies Message-ID: <6BB8BC4519AAB844B174FC739A679BBC1C2FB8@IRMEXCH01.irm.inhs.org> We use Polychrome stain from PolyScientific and it works wonderfully for this purpose -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Thursday, May 11, 2006 10:03 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Marking prostate biopsies We use Toluidine Blue 0.5% alcoholic to mark all our prostate, GI's, and cervical biopsies. The blue color stays through processing. ....................................................................... We are looking for a way to better mark our small needle biopsies such as breast cores and prostate biopsies so that they are more easily seen after processing. ETC. Vickroy Supervisor - MMC Surgical Pathology ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From akbitting <@t> geisinger.edu Thu May 11 13:14:25 2006 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Thu May 11 13:16:30 2006 Subject: [Histonet] IHC on Sakura Xpress processed tissue Message-ID: Can I get feedback from the Histonet world on how IHC staining is effected by switching from standard processing to processing on the Xpress? Does anyone see any other drawbacks to the technology? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From jqb7 <@t> cdc.gov Thu May 11 13:24:39 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/NCID/VR)) Date: Thu May 11 13:29:32 2006 Subject: [Histonet] IHC on Sakura Xpress processed tissue Message-ID: For us there has been no problem at all. In some cases the IHC staining is actually crisper. I will say that we primarily have formalin-fixed tissues (we receive most of our cases from outside CDC). Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, May 11, 2006 2:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC on Sakura Xpress processed tissue Can I get feedback from the Histonet world on how IHC staining is effected by switching from standard processing to processing on the Xpress? Does anyone see any other drawbacks to the technology? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> Mercer.edu Thu May 11 13:29:32 2006 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Thu May 11 13:30:17 2006 Subject: [Histonet] Georgia Society for Histotechnology Message-ID: <01M2ARWEZSA08X4MOD@Macon2.Mercer.edu> The Georgia Society for Histotechnology and the Georgia Society of Cytology will hold their 4th Annual Combined Scientific Symposium on Saturday, June 3, 2006, in Augusta, Georgia at the Marriott Augusta Hotel and Suites, 2 Tenth Street, Augusta, GA 30901. The phone number for reservations is 1-706 722-8900. A program is available upon request to this address. Shirley Powell GSH Secretary/Membership Chair From cwscouten <@t> myneurolab.com Thu May 11 14:15:31 2006 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Thu May 11 14:15:57 2006 Subject: [Histonet] autofluorescence? Message-ID: <5784D843593D874C93E9BADCB87342AB01306F38@tpiserver03.Coretech-holdings.com> Since red blood cells are notorious for autofluroscence, I would guess they are indeed blood vessels, although I have not worked with that material. Is there any way you can sacrifice perfuse the tissue before sectioning? Can you get a needle into a beating heart at that stage? Wash out the red blood cells, and the problem should go away. Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of yasushi nakagawa Sent: Thursday, May 11, 2006 11:13 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] autofluorescence? Hi, We are doing immunohistochemistry using Cy2, Cy3 and Cy5-conjugated secondary antibodies on 20um-thick cryosections of embryonic mouse brains. Especially with Cy2- filter setting, we sometimes see a number of auto-fluorescence-like signals within the brain sections. They are thin and long, kind of looking like blood vessels. They show up even when we do not use Cy2-conjugated secondaries. The same problem has happened to Cy3-filter setting, too, although less frequently. I wonder if anyone has a idea about what they are and how to prevent them from showing up. We fix brains in 4%PFA/PB overnight, wash and cryoprotect in sucrose/ PB, and freeze in OCT in plastic molds on petri dish on liquid nitrogen. We cut 20um cryosections, dry them for an hour or so, and do regular antibody staining, do DAPI staining, dehydrate, and mount in DPX. Thank you for your help. Yasushi Nakagawa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu May 11 14:16:54 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 11 14:16:58 2006 Subject: [Histonet] gluteraldehyde In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA270171FDB5@hpes1.HealthPartners.int> Message-ID: <20060511191654.14736.qmail@web61211.mail.yahoo.com> We used to give it to the same waste management company that disposed of our used NBF Ren? J. "Webb, Dorothy L" wrote: How is everyone disposing if gluteraldehyde in a 3% solution? We obtain ours from Mayo and have some that is outdated, but, they weren't very informative in telling me how to dispose of it. Thanks histonetters for your advice!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- New Yahoo! Messenger with Voice. Call regular phones from your PC and save big. From tpmorken <@t> labvision.com Thu May 11 14:37:12 2006 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Thu May 11 14:37:26 2006 Subject: [Histonet] IHC on Sakura Xpress processed tissue Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA20328D90C@usca0082k08.labvision.apogent.com> Angela, You might want to look at this article in AJCP: Am J Clin Pathol. 2006 Feb;125(2):176-83. Related Articles, Links A comparison of immunohistochemical stain quality in conventional and rapid microwave processed tissues. Emerson LL, Tripp SR, Baird BC, Layfield LJ, Rohr LR. Department of Pathology, University of Utah, Salt Lake City, USA. Same-day turnaround of pathology specimens is desirable in this era of managed care, and rapid microwave tissue processing produces histologic features of a quality equivalent to overnight processing. We studied whether microwave-assisted rapid tissue processing adversely affects the quality of immunohistochemical staining. We selected 30 specimens (20 neoplastic and 10 nonneoplastic) from our routine surgical pathology workload. Paired large tissue blocks were made from each specimen type, one for microwave-assisted rapid processing and one for conventional processing. Two microarrays of 60 punches each were made from the donor blocks. The microarray blocks were examined for intensity and extent of staining by 44 commonly used antibodies. Slides were reviewed independently by 2 pathologists blinded to the type of processing used. In 5,280 tissue punches examined, we found a high degree of concordance in quality, as measured by intensity and extent of immunohistochemical staining, between microwave and routinely processed tissues. Our study demonstrates that quality of immunohistochemical staining is similar between rapid microwave and conventional processing. The potential need for immunohistochemical analysis is not a contraindication for microwave-assisted rapid tissue processing. PMID: 16393680 [PubMed - indexed for MEDLINE] Tim Morken Lab Vision - Neomarkers www.labvision.com Free webhosting for US State Histotechnology Societies: http://www.labvisioncorp.com/demowebsite/index.cfm -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, May 11, 2006 11:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC on Sakura Xpress processed tissue Can I get feedback from the Histonet world on how IHC staining is effected by switching from standard processing to processing on the Xpress? Does anyone see any other drawbacks to the technology? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From omnivore98 <@t> yahoo.com Thu May 11 15:07:58 2006 From: omnivore98 <@t> yahoo.com (Heather Renko) Date: Thu May 11 15:08:01 2006 Subject: [Histonet] prostate and breast core bx's Message-ID: <20060511200758.60112.qmail@web31309.mail.mud.yahoo.com> I have personally used eosin in the 95% step of the processor for years and it really helps with pinking up small cores and prostate bx's without having any effect on the final H&E. We used the premade RA eosin. I have used about 10-20ml in the Tissue Tek VIP which I believe? is about a gallon or so of 95% in the carboy. I started off with a little every time I would change the processor and gradually increased with no complaints from my pathologists. Your embedder will thank you. Heather Renko, HT(ASCP) --------------------------------- Love cheap thrills? Enjoy PC-to-Phone calls to 30+ countries for just 2?/min with Yahoo! Messenger with Voice. From tpmorken <@t> labvision.com Thu May 11 15:14:41 2006 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Thu May 11 15:14:51 2006 Subject: [Histonet] IHC on Sakura Xpress processed tissue Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA20328D910@usca0082k08.labvision.apogent.com> I should mention tha the study below used an Energy Beam Sciences MWP 800 microwave tissue processor, not the Sakura Xpress. Tim -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim - Labvision Sent: Thursday, May 11, 2006 12:37 PM To: 'Angela Bitting'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC on Sakura Xpress processed tissue Angela, You might want to look at this article in AJCP: Am J Clin Pathol. 2006 Feb;125(2):176-83. Related Articles, Links A comparison of immunohistochemical stain quality in conventional and rapid microwave processed tissues. Emerson LL, Tripp SR, Baird BC, Layfield LJ, Rohr LR. Department of Pathology, University of Utah, Salt Lake City, USA. Same-day turnaround of pathology specimens is desirable in this era of managed care, and rapid microwave tissue processing produces histologic features of a quality equivalent to overnight processing. We studied whether microwave-assisted rapid tissue processing adversely affects the quality of immunohistochemical staining. We selected 30 specimens (20 neoplastic and 10 nonneoplastic) from our routine surgical pathology workload. Paired large tissue blocks were made from each specimen type, one for microwave-assisted rapid processing and one for conventional processing. Two microarrays of 60 punches each were made from the donor blocks. The microarray blocks were examined for intensity and extent of staining by 44 commonly used antibodies. Slides were reviewed independently by 2 pathologists blinded to the type of processing used. In 5,280 tissue punches examined, we found a high degree of concordance in quality, as measured by intensity and extent of immunohistochemical staining, between microwave and routinely processed tissues. Our study demonstrates that quality of immunohistochemical staining is similar between rapid microwave and conventional processing. The potential need for immunohistochemical analysis is not a contraindication for microwave-assisted rapid tissue processing. PMID: 16393680 [PubMed - indexed for MEDLINE] Tim Morken Lab Vision - Neomarkers www.labvision.com Free webhosting for US State Histotechnology Societies: http://www.labvisioncorp.com/demowebsite/index.cfm -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, May 11, 2006 11:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC on Sakura Xpress processed tissue Can I get feedback from the Histonet world on how IHC staining is effected by switching from standard processing to processing on the Xpress? Does anyone see any other drawbacks to the technology? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Thu May 11 16:22:51 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu May 11 16:22:59 2006 Subject: [Histonet] Filter papers Message-ID: <09C945920A6B654199F7A58A1D7D1FDE017176F8@lsexch.lsmaster.lifespan.org> Tracey, For filtering such specimens I use Nitex mesh. It's a nylon mesh (under the microscope it looks just like window screen) which can be purchased in a variety of pore sizes. When filtering biopsy materials for histologic processing I usually use a 50 micron mesh. 80 ml of liquid will run through it in a few seconds, and all particles larger then 50 microns will be retained. Finer mesh sizes are available but you probably aren't likely to be processing biopsies smaller then 50 microns. It's more expensive than filter paper but is worth it in time saved. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > Tracey.Lenek@CLS.ab.ca > Sent: Wednesday, May 10, 2006 12:16 PM > To: histonet@lists.utsouthwestern.edu > Cc: Jill.Shupe@CLS.ab.ca > Subject: [Histonet] Filter papers > > We are a large volume lab having to filter colposcopy specimens for > processing. > Having difficulty finding suitable filter paper to accomodate filtering > approx. 80 ml > of fluid per container. We have tried both Fisher and VWR and they both > take far too much time to filter. > Does anyone have a supplier they could recommend? > Thanks in advance. > > Tracey > > > CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain > confidential, personal and/or privileged information intended for a > specific purpose and recipient. If you are not the intended recipient do > not disclose, copy, retain, distribute, use or modify any of the contents > of this transmission. If you received this transmission in error please > notify me immediately by return e-mail or telephone and destroy the entire > transmission and any copies produced. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From weneng2004 <@t> yahoo.com Thu May 11 16:30:09 2006 From: weneng2004 <@t> yahoo.com (wen eng) Date: Thu May 11 16:30:20 2006 Subject: [Histonet] Hemoxygenase-1 IHC Message-ID: <20060511213010.16596.qmail@web53412.mail.yahoo.com> Dear histonetters, May I ask your help to find the best antibody and staining procedure for FFPE rat tissue for the Hemoxygenase-1? I tried many times on Stressgen antibody SPA-895 but couldn't get good stain. Thanks in advance! Wen --------------------------------- Yahoo! Messenger with Voice. PC-to-Phone calls for ridiculously low rates. From jqb7 <@t> cdc.gov Thu May 11 17:24:36 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/NCID/VR)) Date: Thu May 11 17:24:43 2006 Subject: [Histonet] IHC on Sakura Xpress processed tissue References: <0556BE8AC5551E4E8AF6BB9E42509BA20328D910@usca0082k08.labvision.apogent.com> Message-ID: Let me add that before we purchased the Sakura Xpress, I did a very involved study with the Milestone TT/Mega and the effects of microwave technology on IHC staining. The IHC results were also excellent with this machine. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Morken, Tim - Labvision Sent: Thu 5/11/2006 4:14 PM To: Morken, Tim - Labvision; 'Angela Bitting'; histonet@lists.utsouthwestern.edu Cc: Subject: RE: [Histonet] IHC on Sakura Xpress processed tissue I should mention tha the study below used an Energy Beam Sciences MWP 800 microwave tissue processor, not the Sakura Xpress. Tim -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim - Labvision Sent: Thursday, May 11, 2006 12:37 PM To: 'Angela Bitting'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC on Sakura Xpress processed tissue Angela, You might want to look at this article in AJCP: Am J Clin Pathol. 2006 Feb;125(2):176-83. Related Articles, Links A comparison of immunohistochemical stain quality in conventional and rapid microwave processed tissues. Emerson LL, Tripp SR, Baird BC, Layfield LJ, Rohr LR. Department of Pathology, University of Utah, Salt Lake City, USA. Same-day turnaround of pathology specimens is desirable in this era of managed care, and rapid microwave tissue processing produces histologic features of a quality equivalent to overnight processing. We studied whether microwave-assisted rapid tissue processing adversely affects the quality of immunohistochemical staining. We selected 30 specimens (20 neoplastic and 10 nonneoplastic) from our routine surgical pathology workload. Paired large tissue blocks were made from each specimen type, one for microwave-assisted rapid processing and one for conventional processing. Two microarrays of 60 punches each were made from the donor blocks. The microarray blocks were examined for intensity and extent of staining by 44 commonly used antibodies. Slides were reviewed independently by 2 pathologists blinded to the type of processing used. In 5,280 tissue punches examined, we found a high degree of concordance in quality, as measured by intensity and extent of immunohistochemical staining, between microwave and routinely processed tissues. Our study demonstrates that quality of immunohistochemical staining is similar between rapid microwave and conventional processing. The potential need for immunohistochemical analysis is not a contraindication for microwave-assisted rapid tissue processing. PMID: 16393680 [PubMed - indexed for MEDLINE] Tim Morken Lab Vision - Neomarkers www.labvision.com Free webhosting for US State Histotechnology Societies: http://www.labvisioncorp.com/demowebsite/index.cfm -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, May 11, 2006 11:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC on Sakura Xpress processed tissue Can I get feedback from the Histonet world on how IHC staining is effected by switching from standard processing to processing on the Xpress? Does anyone see any other drawbacks to the technology? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.m.vanderloos <@t> amc.uva.nl Fri May 12 01:42:40 2006 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Fri May 12 01:43:00 2006 Subject: [Histonet] RE: double immunostaining Message-ID: <805e52805795.805795805e52@amc.uva.nl> Dear Richard, In collaboration with LabVision I have put the lectures that are part of my multiple staining course on-line: [1]www.labvision.com/indextutorial.cfm. You need to login and then the 1st lecture becomes available. After doing a test the 2nd lecture is available, etc. At this moment there are two tutorials on-line. That will be expanded soon to 6 tutorials all dealing with multiple staining issues. Have fun with it! Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Thu, 11 May 2006 11:47:19 +0100 From: "Edwards, R.E." Subject: [Histonet] double immunostaining To: "histonet" Information please on the preferred kits/systems used for double/triple immunostaining(non-fluorescence), probably using combinations of anti-mouse and rabbit primaries, on paraffin sections. Many thanks &nbs! p; & References 1. http://www.labvision.com/indextutorial.cfm From lpwenk <@t> sbcglobal.net Fri May 12 05:00:37 2006 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri May 12 05:00:48 2006 Subject: [Histonet] Marking prostate biopsies In-Reply-To: <44636433.8040800@u.washington.edu> Message-ID: <001e01c675aa$eb83c790$79162643@HPPav2> I'd be wary of using mercurochrome to mark tissues. Mercurochrome a mercury derivative, specifically dibromohydroxymercurifluoriscein disodium salt (from mercuric acetate and dibromofluoresIcein). Molecular formula: C20 H8 Br2 Hg O6 Na2 The MSDS I've looked at are saying mercurochrome is 24-27% mercury. The dangers listed are that it may be fatal if inhaled, absorbed through the skin or swallowed. Danger of cumulative effects. The target organ is the nervous system. Yes, I know that cuts and scrapes were always treated with this in the past. But remember that treating a scrape once every couple of months is different than a tech/PA/etc. adding it to tissue every day, week after week, being exposed to the liquid and the vapor for long term. Remember, the toxic effects are cumulative. Also, if your hospital/lab has signed the AHA/EPA waste/mercury reduction agreement, this is one of the compounds that needs to be eliminated. So my suggestion is to switch to one of the other methods being discussed by the fine people of this group. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lori Richey Sent: Thursday, May 11, 2006 12:20 PM To: Vickroy, Jim Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Marking prostate biopsies Mercurichrome works well for cardiac bxs. Not sure what percent. Vickroy, Jim wrote: > > >We are looking for a way to better mark our small needle biopsies such >as breast cores and prostate biopsies so that they are more easily seen >after processing. > >Right now the embedder has some trouble identifying the cores and in >turn the histotech often has problems seeing the cores in the paraffin >block. > >We are doing levels on these cases but find that often we need recuts >because we are not into the block enough. > > > >I have heard of some techs who have used a little eosin in their 70% >ETOH, which helped the techs identify the cores. I am reluctant to use >eosin on my automated processors in the 70% ETOH however. We tried >dipping the blocks in eosin tinted formalin at the grossing stations. >The eosin leached out and did no good. Does anyone have any good >ideas? > > > >I do know that some send eosin tinted formalin to sites that are doing >these biopsies. I don't see that as an option for us. Thanks for your >help. > > > >James R. Vickroy > >Supervisor - MMC Surgical Pathology > >217-788-4046 > > > > > >This message (including any attachments) contains confidential >information intended for a specific individual and purpose, and is >protected by law. If you are not the intended recipient, you should >delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From peter_bannister <@t> hotmail.co.uk Fri May 12 05:27:37 2006 From: peter_bannister <@t> hotmail.co.uk (Peter Bannister) Date: Fri May 12 05:27:46 2006 Subject: [Histonet] RE: autofluorescence In-Reply-To: Message-ID: Dear Yasushi. I have experienced the same autofluorescence in brain tissue and it does sound like you are indeed seeing blood vessels. Try incubating the sections in a solution of 1% Sodium Borohydride (NaBH4) in 0.1M Phosphate buffer (not PBS) for about 30 minutes. This substance will quench autofluorescnce from free aldehyde groups left over from fixation and should eradicate the problem. If you are using sections on slides then the use of salinated (eg vectabond) coated slide will help retain section adhesion. If you are using free flating sections then remember not to cap the vial during the incubation. The reason is that NaBH4 produces a lot of hydrogen gas when in solution and the pressure in the vial will build up and eventually blow the cap off and stick the sections to the laboratory wall. This is bad. Also remember that NaBH4 is pretty nasty stuff when in powdered form so take all necessary health and safety precautions. Good Luck. Peter Bannister. > >Message: 17 >Date: Thu, 11 May 2006 11:12:51 -0500 >From: yasushi nakagawa >Subject: [Histonet] autofluorescence? >To: Histonet@lists.utsouthwestern.edu >Message-ID: >Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed > >Hi, > >We are doing immunohistochemistry using Cy2, Cy3 and Cy5-conjugated >secondary antibodies on 20um-thick cryosections of embryonic mouse >brains. Especially with Cy2- filter setting, we sometimes see a >number of auto-fluorescence-like signals within the brain sections. >They are thin and long, kind of looking like blood vessels. They show >up even when we do not use Cy2-conjugated secondaries. The same >problem has happened to Cy3-filter setting, too, although less >frequently. I wonder if anyone has a idea about what they are and how >to prevent them from showing up. > >We fix brains in 4%PFA/PB overnight, wash and cryoprotect in sucrose/ >PB, and freeze in OCT in plastic molds on petri dish on liquid >nitrogen. We cut 20um cryosections, dry them for an hour or so, and >do regular antibody staining, do DAPI staining, dehydrate, and mount >in DPX. > >Thank you for your help. > >Yasushi Nakagawa > _________________________________________________________________ The new MSN Search Toolbar now includes Desktop search! http://join.msn.com/toolbar/overview From jnocito <@t> satx.rr.com Fri May 12 05:55:14 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri May 12 05:55:21 2006 Subject: [Histonet] Dako References: Message-ID: <002001c675b2$8ce2a550$1bb30b43@yourxhtr8hvc4p> Glen, I understand the computer changes, but it would have been better if Dako sent out a letter to their customers explaining the situation, instead of us coming up with our own juicy stories. Wait a minute. What am I talking about? Let's stay with the juicy stories. They're more thought provoking. Joe "The Toe" Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Dawson, Glen" To: Sent: Thursday, May 11, 2006 8:06 AM Subject: RE: [Histonet] Dako All, Dako has been undergoing an Oracle computer system change & I've noticed the bugz myself. I am currently undergoing a changeover to Cerner Millenium in my lab so I may have more sympathy for them than most because we're in the same foxhole. As with my lab, I'm sure things will run much smoother a little ways down the road. With Sympathy, Glen Dawson BS, HT & QIHC IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.392 / Virus Database: 268.5.5/335 - Release Date: 5/9/2006 From jnocito <@t> satx.rr.com Fri May 12 05:58:04 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri May 12 05:58:09 2006 Subject: [Histonet] Marking prostate biopsies References: <4D55B2E997EFAE4DA6081DDE100B8302DE91FD@amedmlsermc133> Message-ID: <002e01c675b2$f244ee30$1bb30b43@yourxhtr8hvc4p> I'm with Diane, we also use eosin in our last 100% ETOH and haven't had problems and we have new graduates embedding biopsies. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Gladney, Diane C Ms MACH" To: "Vickroy, Jim" ; Sent: Thursday, May 11, 2006 9:36 AM Subject: RE: [Histonet] Marking prostate biopsies I use Eosin in my last 100% Ethanol on my tissue processor. It works great for the small biopsies of other tissue also. We have not had any problems seeing these small biopsies or needle core biopsies since I started doing this. Diane Diane C. Gladney, HT (ASCP) Supervisor, Histology Dept. Moncrief Army Community Hospital Dept. of Pathology 4500 Stuart St. FT. Jackson, SC 29207 Email: diane.gladney@se.amedd.army.mil Phone: 803-751-2530 FAX: 803-751-7829 DSN: 734-2530 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Thursday, May 11, 2006 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Marking prostate biopsies We are looking for a way to better mark our small needle biopsies such as breast cores and prostate biopsies so that they are more easily seen after processing. Right now the embedder has some trouble identifying the cores and in turn the histotech often has problems seeing the cores in the paraffin block. We are doing levels on these cases but find that often we need recuts because we are not into the block enough. I have heard of some techs who have used a little eosin in their 70% ETOH, which helped the techs identify the cores. I am reluctant to use eosin on my automated processors in the 70% ETOH however. We tried dipping the blocks in eosin tinted formalin at the grossing stations. The eosin leached out and did no good. Does anyone have any good ideas? I do know that some send eosin tinted formalin to sites that are doing these biopsies. I don't see that as an option for us. Thanks for your help. James R. Vickroy Supervisor - MMC Surgical Pathology 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.392 / Virus Database: 268.5.5/335 - Release Date: 5/9/2006 From jnocito <@t> satx.rr.com Fri May 12 06:01:10 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri May 12 06:01:16 2006 Subject: [Histonet] STAFF EVALUATIONS References: <20060511154403.18424.qmail@web61219.mail.yahoo.com> Message-ID: <004101c675b3$613a14f0$1bb30b43@yourxhtr8hvc4p> I created a competency test for every section of my lab, routine, special stains, immuno, sucking up to the manager, etc. the scores are reflected on their annual evals. This way, the evals are not strictly subjective. It's not perfect, but it helps. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Rene J Buesa" To: "Diana McCaig" ; "Histonet (E-mail)" Sent: Thursday, May 11, 2006 10:44 AM Subject: Re: [Histonet] STAFF EVALUATIONS > Once a year to all staff member and tasks. During probation there were > monthly evaluations. > Ren? J. > > Diana McCaig wrote: > Does anyone do an annual evaluation of staff ability to perform certain > tasks? > Diana McCaig 519-352-6401 (6604) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Love cheap thrills? Enjoy PC-to-Phone calls to 30+ countries for just > 2?/min with Yahoo! Messenger with Voice. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.392 / Virus Database: 268.5.5/335 - Release Date: 5/9/2006 > > From DDDeltour <@t> mar.med.navy.mil Fri May 12 06:04:23 2006 From: DDDeltour <@t> mar.med.navy.mil (Deltour, Douglas D. (HM2)) Date: Fri May 12 06:04:57 2006 Subject: [Histonet] Dako Message-ID: <3F500F8B416C554EBB21FF16642F72E959CE8A@marxchg03.mar.med.navy.mil> I hear that they are in the process of being bought out by Fisher Scientific or whatever it is called today. :) D -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Friday, May 12, 2006 6:55 AM To: Dawson, Glen; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Dako Glen, I understand the computer changes, but it would have been better if Dako sent out a letter to their customers explaining the situation, instead of us coming up with our own juicy stories. Wait a minute. What am I talking about? Let's stay with the juicy stories. They're more thought provoking. Joe "The Toe" Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Dawson, Glen" To: Sent: Thursday, May 11, 2006 8:06 AM Subject: RE: [Histonet] Dako All, Dako has been undergoing an Oracle computer system change & I've noticed the bugz myself. I am currently undergoing a changeover to Cerner Millenium in my lab so I may have more sympathy for them than most because we're in the same foxhole. As with my lab, I'm sure things will run much smoother a little ways down the road. With Sympathy, Glen Dawson BS, HT & QIHC IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.392 / Virus Database: 268.5.5/335 - Release Date: 5/9/2006 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Fri May 12 06:35:33 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri May 12 06:36:27 2006 Subject: [Histonet] Dako References: <3F500F8B416C554EBB21FF16642F72E959CE8A@marxchg03.mar.med.navy.mil> Message-ID: THAT is just a rumor! @:) ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Deltour, Douglas D. (HM2) Sent: Fri 5/12/2006 7:04 AM To: 'Joe Nocito'; Dawson, Glen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako I hear that they are in the process of being bought out by Fisher Scientific or whatever it is called today. :) D -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Friday, May 12, 2006 6:55 AM To: Dawson, Glen; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Dako Glen, I understand the computer changes, but it would have been better if Dako sent out a letter to their customers explaining the situation, instead of us coming up with our own juicy stories. Wait a minute. What am I talking about? Let's stay with the juicy stories. They're more thought provoking. Joe "The Toe" Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Dawson, Glen" To: Sent: Thursday, May 11, 2006 8:06 AM Subject: RE: [Histonet] Dako All, Dako has been undergoing an Oracle computer system change & I've noticed the bugz myself. I am currently undergoing a changeover to Cerner Millenium in my lab so I may have more sympathy for them than most because we're in the same foxhole. As with my lab, I'm sure things will run much smoother a little ways down the road. With Sympathy, Glen Dawson BS, HT & QIHC IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.392 / Virus Database: 268.5.5/335 - Release Date: 5/9/2006 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DDDeltour <@t> mar.med.navy.mil Fri May 12 06:40:36 2006 From: DDDeltour <@t> mar.med.navy.mil (Deltour, Douglas D. (HM2)) Date: Fri May 12 06:41:01 2006 Subject: [Histonet] Dako Message-ID: <3F500F8B416C554EBB21FF16642F72E959CE8B@marxchg03.mar.med.navy.mil> So you have heard it too??? I thought I just invented it. See how fast these things get around. :-) _____ From: Bonner, Janet [mailto:Janet.Bonner@FLHOSP.ORG] Sent: Friday, May 12, 2006 7:36 AM To: Deltour, Douglas D. (HM2); Joe Nocito; Dawson, Glen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako THAT is just a rumor! @:) _____ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Deltour, Douglas D. (HM2) Sent: Fri 5/12/2006 7:04 AM To: 'Joe Nocito'; Dawson, Glen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Dako I hear that they are in the process of being bought out by Fisher Scientific or whatever it is called today. :) D -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com ] Sent: Friday, May 12, 2006 6:55 AM To: Dawson, Glen; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Dako Glen, I understand the computer changes, but it would have been better if Dako sent out a letter to their customers explaining the situation, instead of us coming up with our own juicy stories. Wait a minute. What am I talking about? Let's stay with the juicy stories. They're more thought provoking. Joe "The Toe" Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Dawson, Glen" To: Sent: Thursday, May 11, 2006 8:06 AM Subject: RE: [Histonet] Dako All, Dako has been undergoing an Oracle computer system change & I've noticed the bugz myself. I am currently undergoing a changeover to Cerner Millenium in my lab so I may have more sympathy for them than most because we're in the same foxhole. As with my lab, I'm sure things will run much smoother a little ways down the road. With Sympathy, Glen Dawson BS, HT & QIHC IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.392 / Virus Database: 268.5.5/335 - Release Date: 5/9/2006 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lesley.bechtold <@t> jax.org Fri May 12 06:57:33 2006 From: lesley.bechtold <@t> jax.org (Lesley Bechtold) Date: Fri May 12 06:58:44 2006 Subject: [Histonet] STAFF EVALUATIONS In-Reply-To: <004101c675b3$613a14f0$1bb30b43@yourxhtr8hvc4p> Message-ID: <20060512075733376.00000005864@spikey> Any chance you'd share that? I'm curious, particularly the "sucking up to the manager" rating!!!! But seriously, I would be interested. Thanks. Lesley Lesley S. Bechtold Senior Manager, Histopathology & Microscopy Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Friday, May 12, 2006 7:01 AM To: Rene J Buesa; Diana McCaig; Histonet (E-mail) Subject: Re: [Histonet] STAFF EVALUATIONS I created a competency test for every section of my lab, routine, special stains, immuno, sucking up to the manager, etc. the scores are reflected on their annual evals. This way, the evals are not strictly subjective. It's not perfect, but it helps. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Rene J Buesa" To: "Diana McCaig" ; "Histonet (E-mail)" Sent: Thursday, May 11, 2006 10:44 AM Subject: Re: [Histonet] STAFF EVALUATIONS > Once a year to all staff member and tasks. During probation there were > monthly evaluations. > Ren? J. > > Diana McCaig wrote: > Does anyone do an annual evaluation of staff ability to perform certain > tasks? > Diana McCaig 519-352-6401 (6604) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Love cheap thrills? Enjoy PC-to-Phone calls to 30+ countries for just > 2?/min with Yahoo! Messenger with Voice. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.392 / Virus Database: 268.5.5/335 - Release Date: 5/9/2006 > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wellborj <@t> mercyhealth.com Fri May 12 08:01:15 2006 From: wellborj <@t> mercyhealth.com (Janci Wellborn) Date: Fri May 12 08:01:51 2006 Subject: [Histonet] IHC on Sakura Xpress processed tissue Message-ID: How did you do the compairson for IHC control tissue? Don't the control tissues have to be treated the same as the patients? When you switched from regular processing to microwave, did you do any compairsons between the two forms of processing? Janci Wellborn, BS, BSeD, HTL (ASCP) Dunes Medical Laboratory Dakota Dunes, SD wellborj@mercyhealth.com >>> "Bartlett, Jeanine (CDC/NCID/VR)" 5/11/2006 1:24 PM >>> For us there has been no problem at all. In some cases the IHC staining is actually crisper. I will say that we primarily have formalin-fixed tissues (we receive most of our cases from outside CDC). Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, May 11, 2006 2:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC on Sakura Xpress processed tissue Can I get feedback from the Histonet world on how IHC staining is effected by switching from standard processing to processing on the Xpress? Does anyone see any other drawbacks to the technology? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From yeepengtiang <@t> hotmail.com Fri May 12 08:09:44 2006 From: yeepengtiang <@t> hotmail.com (tiang yeepeng) Date: Fri May 12 08:09:50 2006 Subject: [Histonet] (no subject) Message-ID: Hi all, Can some one tell what are Hep G2 cells? Are they cancerous cells like HeLa or normal cells? Thanks. Kelcy Department of Medical Microbiology, University of Malaya, Malaysia. _________________________________________________________________ Join the next generation of Hotmail and you could win the adventure of a lifetime http://www.imagine-msn.com/minisites/sweepstakes/mail/register.aspx From jqb7 <@t> cdc.gov Fri May 12 08:11:11 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/NCID/VR)) Date: Fri May 12 08:13:02 2006 Subject: [Histonet] IHC on Sakura Xpress processed tissue Message-ID: I had the pathologists routinely gross two sets of tissues for me (when there was enough tissue), one for traditional processing and the other for the Xpress. IHC testing on the tissues processed by both methods was run in the same experiment. In the beginning, the controls we used were not microwave processed but if the diagnostic cases came back positive by both routine and microwave processing then it was apparent that there was not a problem. We have since be working on cell culture controls and testing them to see how the microwave processing affects them. We make ours in Histogel and since cell controls require less time in the Xpress pre-processing solution I have trying to get that optimized to avoid over-hardening. Jeanine Bartlett, BS, HT(ASCP) (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janci Wellborn Sent: Friday, May 12, 2006 9:01 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] IHC on Sakura Xpress processed tissue How did you do the compairson for IHC control tissue? Don't the control tissues have to be treated the same as the patients? When you switched from regular processing to microwave, did you do any compairsons between the two forms of processing? Janci Wellborn, BS, BSeD, HTL (ASCP) Dunes Medical Laboratory Dakota Dunes, SD wellborj@mercyhealth.com >>> "Bartlett, Jeanine (CDC/NCID/VR)" 5/11/2006 1:24 PM >>> For us there has been no problem at all. In some cases the IHC staining is actually crisper. I will say that we primarily have formalin-fixed tissues (we receive most of our cases from outside CDC). Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, May 11, 2006 2:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC on Sakura Xpress processed tissue Can I get feedback from the Histonet world on how IHC staining is effected by switching from standard processing to processing on the Xpress? Does anyone see any other drawbacks to the technology? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From plucas <@t> biopath.org Fri May 12 10:38:51 2006 From: plucas <@t> biopath.org (Paula Lucas) Date: Fri May 12 10:42:01 2006 Subject: [Histonet] HPV Typing Message-ID: <000601c675da$2c0fef80$7c01a8c0@biopath.org> Hello, Could someone please advise us on HPV typing. We received a request for HPV typing on tissue sent to us fixed in 10% NBF and our questions are: can it be done on formalin fixed tissue, what is the procedure (stain?), is it sent out to what entity (a genzyme, a Unilab)? Thanks, Paula Bio-Path Medical Group From GauchV <@t> mail.amc.edu Fri May 12 10:48:55 2006 From: GauchV <@t> mail.amc.edu (Vicki Gauch) Date: Fri May 12 10:51:10 2006 Subject: [Histonet] HPV Typing Message-ID: Paula, We receive these requests also. We send them out to Lab Corp, RTP, North Carolina. Our specimens come in formalin and we process them for routine histology and then cut unstained slides for the HPV testing. Hope that helps, Vicki AMCH Albany, NY >>> "Paula Lucas" 5/12/2006 11:38:51 AM >>> Hello, Could someone please advise us on HPV typing. We received a request for HPV typing on tissue sent to us fixed in 10% NBF and our questions are: can it be done on formalin fixed tissue, what is the procedure (stain?), is it sent out to what entity (a genzyme, a Unilab)? Thanks, Paula Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. From Rcartun <@t> harthosp.org Fri May 12 11:06:30 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri May 12 11:06:58 2006 Subject: [Histonet] HPV Typing In-Reply-To: <000601c675da$2c0fef80$7c01a8c0@biopath.org> References: <000601c675da$2c0fef80$7c01a8c0@biopath.org> Message-ID: <44647A4602000009000AE806@hcnwgwds01.hh.chs> Yes, HPV typing can be performed on formalin-fixed, paraffin-embedded tissue. Although monoclonal antibodies to HPV type-specific proteins exist, I recommend that you do in situ DNA hybridization for definitive identification and typing. I have used ProPath (Dallas, TX) and Genzyme/Impath for this in the past. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Paula Lucas" 05/12/06 11:38 AM >>> Hello, Could someone please advise us on HPV typing. We received a request for HPV typing on tissue sent to us fixed in 10% NBF and our questions are: can it be done on formalin fixed tissue, what is the procedure (stain?), is it sent out to what entity (a genzyme, a Unilab)? Thanks, Paula Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GauchV <@t> mail.amc.edu Fri May 12 11:46:14 2006 From: GauchV <@t> mail.amc.edu (Vicki Gauch) Date: Fri May 12 11:45:54 2006 Subject: [Histonet] Formalin vs. Saline Specimen Submission Message-ID: Our frozen section room is located in the OR so we receive all of our specimens fresh. The Histotech assigned to the FS room is the one responsible for placing specimens in formalin (or other appropriate fixative for special testing) except after hours. The OR staff then places the specimens in formalin if FS room staff has gone for the day. Any question on tissue for special studies the OR staff pages the tech on call or pathologist on call for direction. We do have a grossing station that has ventilation in case we need it . For specimens submitted in saline....if during the day, the resident assigned to the service for which that specimen was intended is paged immediately for triaging of the case. If after hours, the specimen would most likely be placed in the refrigerator overnight and picked up first thing in the morning. Generally, if the specimen is to be handled right away they generally put enough saline on the tissue to keep it wet. If it is going to sit longer than that, more saline would be used. Specimens that need to be refrigerated for any reason are stored in the grossing room refrigerator or Morgue cooler (if too large for the fridge) and after hours,in the fridge in our lab receiving area. After hours, I mentioned how the OR handles their specimens for us...if there are frozen sections involved, the pathologist on call would be paged to come in and handle the case. If the specimen in question comes from an outpatient source, our lab client service center would call the tech on call to see how to handle fresh or saline soaked specimens. We do have them refrigerate saline soaked specimens overnight depending on the testing to be performed. Phew...that was a long one !!! Hope it helps.. Vicki Gauch AMCH >>> "O'Brien, Sue" 5/11/2006 12:51:33 PM >>> I was wondering how other hospitals handled specimens submitted from the OR (Operating Room). Currently, our routine specimens (ones that do not require special testing and submission) are submitted in 10% NBF (Neutral Buffered Formalin). Thank-you for taking the time to respond! How are routine specimens submitted to your lab from the OR (chose one)? a. in formalin (e.g. 10% NBF) b. in saline c. fresh d. other (specify): A. For specimens are received by OR in formalin: Is the specimen placed in formalin in the OR procedure room? If yes, how is it done? a. under a fume hood b. placed in container which has formalin c. other (specify): If no, how is it done? a. specimen placed in container and taken fresh to another room where formalin is added b. other (specify): Does the OR have a fume hood available for use (to put specimens into formalin under it)? B. For specimens submitted by OR in saline: a. How long (generally) is the specimen in saline until it is placed into formalin? b. What volume of saline is used? (e.g. enough to keep moist, or enough to submerge specimen)? c. Who transfers the specimen into formalin? (e.g. histo or lab tech at time of receipt, or does it wait until the Pathologist grosses it? If it waits for the Pathologist, then for how long, and how is it refrigerated?) d. Are specimens refrigerated? If yes, where (e.g. in OR, in lab, in histology). C. For specimens submitted fresh: a. how long (generally) is the specimen fresh before placement into formalin? b. How do you keep the small specimens from drying out? c. Who transfers the specimen into formalin? (e.g. histo or tech at time of receipt, or does it wait until the Pathologist grosses it? If it waits for the Pathologist, then for how long, and how is it refrigerated?) d. Are specimens refrigerated? If yes, where (e.g. in OR, in lab, in histology). If you receive specimens either in saline or fresh (and they are not refrigerated immediately): a. How do you feel they compare to specimens that were immediately placed into formalin? (e.g. better morphology, worse morphology, etc). b. Who handles specimens submitted after hours? (e.g. weekends/holidays; are they left at room temp, refrigerated, or does someone (who?) transfer them into formalin (how?). Sorry for the length, but I would really appreciate knowing how others handle this. Sincerely, Susan O'Brien, Histology Supervisor Burdette Tomlin Memorial Hospital Cape May Court House, NJ 08210 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. From Melissa.Gonzalez <@t> cellgenesys.com Fri May 12 13:19:07 2006 From: Melissa.Gonzalez <@t> cellgenesys.com (Melissa Gonzalez) Date: Fri May 12 13:19:14 2006 Subject: [Histonet] collagen IHC on mouse Message-ID: In addition to Trichrome, which antibodies would be best to detect collagen content in sub cutaneous xenografts for double immunostaining? Since there are Types I-IV, I wasn't sure which, if any, would be best. (Excluding mouse monoclonals, please) Thanks in advance, Melissa From CBark <@t> memorialcare.org Fri May 12 14:13:39 2006 From: CBark <@t> memorialcare.org (Christine R Bark) Date: Fri May 12 14:14:29 2006 Subject: [Histonet] Re: Marking Prostate Bx's Message-ID: <59FE4C55358C6A42B84BAEC6092CEDBC0C0CADD4@sbnt7.memnet.org> Our pathologists prefer inking the small stuff with 0.25% alcoholic basic fuchsin solution. Christine Bark Senior Histotech, Pathology Saddleback Memorial Medical Center 949-452-3548 cbark@memorialcare.org From NKMitche <@t> ahs.llumc.edu Fri May 12 15:07:04 2006 From: NKMitche <@t> ahs.llumc.edu (Mitchell, Nancy K.) Date: Fri May 12 15:07:08 2006 Subject: [Histonet] Re: Marking Prostate Bx's Message-ID: <8EB1459EA20E4E498AC2E05378F8F30A536F42@mind.mc.ad.lluahsc.org> We prefer putting 20cc of (Alc. Eosin) in the first 100% alcohol station of our processor. We find that this method gives all of our skin margins enough color to not only aid the tech that is embedding, it also saves one step for our tech that does the grossing. The color is slightly opaque and does not interfere with the H & E stain. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Christine R Bark Sent: Friday, May 12, 2006 12:14 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Marking Prostate Bx's Our pathologists prefer inking the small stuff with 0.25% alcoholic basic fuchsin solution. Christine Bark Senior Histotech, Pathology Saddleback Memorial Medical Center 949-452-3548 cbark@memorialcare.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Note: The preceding e-mail message (including any attachments) contains information that may be confidential, protected by applicable legal privileges, or constitute non-public information. It is intended to be conveyed only to the designated recipient(s). If you are not an intended recipient of this message, please notify the sender by replying to this message and then delete it from your system. Use, dissemination, distribution or reproduction of this message by unintended recipients is not authorized and may be unlawful. From eric <@t> ategra.com Fri May 12 15:54:20 2006 From: eric <@t> ategra.com (Eric Dye) Date: Fri May 12 15:42:06 2006 Subject: [Histonet] Correction to my last email (Subject should have been Histology) Message-ID: <20060512204155.JUKI144.rrcs-fep-10.hrndva.rr.com@Pamsgx110> Hi - I wanted to update you that we have filled positions 4 and 8 - I have brand new jobs for HistoTech Supervisors and Histo Bench Techs. If you have received this e-mail more than once I sincerely apologize for the duplication. These are mostly fulltime permanent positions. Most of my Histo Techs positions are clinical, although I do have a few Temporary and Research Histo Tech positions as well. Most Histo openings are dayshift (Except where indicated below) Here are some of my Hottest Histology Openings: 1. Northern New Jersey (Full-time, Perm and Temp (2 openings), Bench Histo Tech, 1st shift) 2. Rhode Island - Providence (Full-time, Perm, Bench Histo Tech, 2nd Shift) 4. filled 5. Ohio (Southwestern Ohio) (Full-time, Perm and Temp (2 openings), Bench Histo Tech, 1st shift) 6. New York (Long Island) (Full-time, Perm, SUPERVISOR Histo Tech) 7. Virginia(South - Coastal) (Full-time, Perm, Bench Histo Tech) 8. filled 9. South Florida (Full-time, Perm, Supervisor Histo Tech) 10. South Florida (Full-time, Perm, Bench Histo Tech) 11. Mass (Boston) (Part-time, Perm, Bench Histo Tech) 12. West Virginia (Full-time, Perm, Bench Histo Tech) 13. Texas (Austin area) (Full-time, Perm, Supervisor and Bench Histo Tech) 14. Georgia (Atlanta area) (Full-time, Perm, Bench Histotech) 15. New Hampshire (Mohs and Routine Bench Histotech,Perm and Temp) 16. Florida. (South East Coast, Perm, Bench Histotech) 17. Florida. (Central East Coast, Temp, Bench Histo Tech) 18. Ohio (Central) (Temp, Bench Histotech) 19. New Jersey (Northern) (Temp, Bench Tech) 20. South Carolina (Charleston Area) (Fulltime, Perm, Dermpath only) 21. Virginia (South Beltway area) (Fulltime , perm, Histology Supervisor) The clients are currently interviewing Histology candidates - so if you are interested, please call me today at 1-800-466-9919 ext 223 or on my cell phone at 407-756-5507. Thank you, Eric Dye-Sr Allied Healthcare Recruiter 1-800-466-9919 ext 223 or Cell - 407-756-5507 --------------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing 800 466 9919 ext 223 - voice 407 671 6075 - fax eric@ategra.com To Learn More About Ategra: http://www.ategra.com ---------------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. ---------------------------------------------------------------- From vonavi <@t> inbox.lv Fri May 12 16:15:08 2006 From: vonavi <@t> inbox.lv (Andrejs Ivanovs) Date: Fri May 12 16:15:15 2006 Subject: [Histonet] Technovit 9100 New and Technovit 4000 Message-ID: <1147468508.4464fadc1fd40@www.inbox.lv> Hello, In our lab, we are working with EXAKT Cutting/Grinding system. For tissue embedding and polymerization we are going to use Technovit 9100 New embedding medium. We have never done it before! To attach the plastic block to a slide we are going to use Technovit 4000. Will it be ok to use Technovit 4000 in this case? Simply, before we used other materials… Thank you very much! Best regards, Andrey Ivanov From htlablady <@t> yahoo.com Fri May 12 16:38:40 2006 From: htlablady <@t> yahoo.com (Histo Lady) Date: Fri May 12 16:38:46 2006 Subject: [Histonet] Help! Message-ID: <20060512213840.40982.qmail@web38605.mail.mud.yahoo.com> I don't know what else to do. I am the pathology supervisor and have a histo tech that continuously mislabels cassettes and slides. Fortunately we have been able to fix everything, so far. Yeah I know at some point we won't be able to. This has been going on for sometime. Each time I tell her that she mislabeled something and each time I tell my manager and document it. The manager and HR have spoken to her on 3 different occasions. Yes I said 3 and she has mislabeled cassettes and/or slides more times then that. And yet she still has a job. I have tried everything short of sitting behind her all day and watch everything she does to make sure she does it right. No one wants to take action on this issue except me and I can't do anything other then document it and tell her every time. And of course since no one else is doing anything about it I am the bad guy for "telling on her". The pathologists agree with me that something needs done and they all have told the manager to do something. But they don't want to get involved because "personal issues are not thier issues". I don't know what else to do. __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From JWEEMS <@t> sjha.org Fri May 12 16:52:35 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri May 12 16:52:42 2006 Subject: [Histonet] Help! References: <20060512213840.40982.qmail@web38605.mail.mud.yahoo.com> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD320263E0AB@sjhaexc02.sjha.org> This is a very difficult situation to have to deal with, but I would think this is a top patient care issue that should be addressed in a progressive discipline manner. HR should have that in place so that after a set number of instances, certain things happen up to and including termination. At the written warning stage, a plan would be devised to determine if there is something causing the errors - can the tech see well, etc. If errors are made that leave the department, Joint Commission requires a review of the incident to determine what can be done to improve quality and eliminate that type of errors. The object of the exercise is not to intimidate or torture the employee but help them become the best tech doing the best patient care possible. I wish you the best! j Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 404-851-7831 - Fax ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Histo Lady Sent: Fri 5/12/2006 5:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help! I don't know what else to do. I am the pathology supervisor and have a histo tech that continuously mislabels cassettes and slides. Fortunately we have been able to fix everything, so far. Yeah I know at some point we won't be able to. This has been going on for sometime. Each time I tell her that she mislabeled something and each time I tell my manager and document it. The manager and HR have spoken to her on 3 different occasions. Yes I said 3 and she has mislabeled cassettes and/or slides more times then that. And yet she still has a job. I have tried everything short of sitting behind her all day and watch everything she does to make sure she does it right. No one wants to take action on this issue except me and I can't do anything other then document it and tell her every time. And of course since no one else is doing anything about it I am the bad guy for "telling on her". The pathologists agree with me that something needs done and they all have told the manager to do something. But they don't want to get involved because "personal issues are not thier issues". I don't know what else to do. __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From michael.owen <@t> fda.hhs.gov Fri May 12 17:35:17 2006 From: michael.owen <@t> fda.hhs.gov (Owen, Michael P) Date: Fri May 12 17:36:00 2006 Subject: FW: [Histonet] Help! Message-ID: From: Owen, Michael P Sent: Friday, May 12, 2006 3:35 PM To: 'Histo Lady' Subject: RE: [Histonet] Help! Dear Histo Lady, The best approach to take is to apply CAPA testing to the situation. Determine the problem, create a corrective action then implement preventive action. My colleagues target CAPA files for critical reviews in biomedical firms during inspections as most members of this list know. Sadly, my colleagues are slow to applying this concept to their own work. :o) The employee may not be the problem, instead you may have a system that needs adjustment to work smarter and not harder. Good luck in your investigation. Michael P. Owen, Regulatory Microbiologist U.S. FDA Pacific Regional Lab Northwest 22201 23rd Drive SE Bothell, WA 98021-4421 Phone: 425-483-4865 E-Mail: michael.owen@fda.hhs.gov From vonavi <@t> inbox.lv Sat May 13 00:40:04 2006 From: vonavi <@t> inbox.lv (Andrejs Ivanovs) Date: Sat May 13 00:40:10 2006 Subject: [Histonet] Technovit 9100 NEW and Technovit 4000 Message-ID: <1147498804.44657134a2ce7@www.inbox.lv> Hello, In our lab, we are working with EXAKT Cutting/Grinding system. For tissue embedding and polymerization we are going to use Technovit 9100 New embedding medium. We have never done it before! To attach the plastic block to a slide we are going to use Technovit 4000. Will it be ok to use Technovit 4000 in this case? Simply, before we used other materials. Thank you very much! Best regards, Andrey Ivanov From Maxim_71 <@t> mail.ru Sat May 13 13:41:12 2006 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Sat May 13 13:42:38 2006 Subject: [Histonet] Manual processing In-Reply-To: <1147376541.1014012607@mx11.mail.ru> References: <1147376541.1014012607@mx11.mail.ru> Message-ID: <14012099515.20060513224112@mail.ru> We have routine manually processing shedule for most specimens: Fixation in 10% NBF overnight. Dehydratation in acetone 5 x 30 min. Intermediate step is acetone / white-spirit (substitute of xylene) 1:1 60 min Clearing in white-spirit 4 x 30 min and leave here before morning. Infiltration in paraffin 4 x 60 min, then embedding, cut, stain, coverslip in this or next workday. All procedures we do manually. Best regards, Maxim Peshkov Histotechnologist, Taganrog Russia. From ltravis9 <@t> cox.net Sat May 13 13:39:30 2006 From: ltravis9 <@t> cox.net (Lisa Travis) Date: Sat May 13 13:47:42 2006 Subject: [Histonet] Grossing Techs Message-ID: <005d01c676bd$b40f0ea0$030aa8c0@lisascomputer> Can anyone tell me if there is a set pay scale for HTs that do some of the grossing? From anh2006 <@t> med.cornell.edu Sat May 13 23:29:33 2006 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Sat May 13 23:28:47 2006 Subject: [Histonet] DAKO - Target Retrieval Solution Message-ID: After all this talk about DAKO I am worried ... I live for DAKO's Target Retrieval Solution. In my opinion, it is simply the best out there and gives me fantastic results every time I use it. Love it! So in case I cannot get my hands on more for some time (last time I ordered it was very backordered), I need to consider other options .... ... ... So my question is, what commercially available solutions (besides those from DAKO) do people rely on for heat retrieval and what are your thoughts on them? THANK YOU! -- From rjbuesa <@t> yahoo.com Sun May 14 08:00:38 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun May 14 08:00:48 2006 Subject: [Histonet] DAKO - Target Retrieval Solution In-Reply-To: Message-ID: <20060514130038.5898.qmail@web61212.mail.yahoo.com> I always prepared my own antigen retrieval solutions (pH 6 and pH 8). Used them always sucessfully. If interested I can send you the recipes. Ah, it is 10 times cheaper also! Ren? J. "Andrea T. Hooper" wrote: After all this talk about DAKO I am worried ... I live for DAKO's Target Retrieval Solution. In my opinion, it is simply the best out there and gives me fantastic results every time I use it. Love it! So in case I cannot get my hands on more for some time (last time I ordered it was very backordered), I need to consider other options .... ... ... So my question is, what commercially available solutions (besides those from DAKO) do people rely on for heat retrieval and what are your thoughts on them? THANK YOU! -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Love cheap thrills? Enjoy PC-to-Phone calls to 30+ countries for just 2?/min with Yahoo! Messenger with Voice. From Luis.Chiriboga <@t> med.nyu.edu Sun May 14 10:02:13 2006 From: Luis.Chiriboga <@t> med.nyu.edu (Luis Chiriboga) Date: Sun May 14 14:39:28 2006 Subject: [Histonet] Help! In-Reply-To: <20060512213840.40982.qmail@web38605.mail.mud.yahoo.com> Message-ID: is she dyslexic? if so then maybe she can be reassigned.....???? LC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Histo Lady Sent: Friday, May 12, 2006 5:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Help! I don't know what else to do. I am the pathology supervisor and have a histo tech that continuously mislabels cassettes and slides. Fortunately we have been able to fix everything, so far. Yeah I know at some point we won't be able to. This has been going on for sometime. Each time I tell her that she mislabeled something and each time I tell my manager and document it. The manager and HR have spoken to her on 3 different occasions. Yes I said 3 and she has mislabeled cassettes and/or slides more times then that. And yet she still has a job. I have tried everything short of sitting behind her all day and watch everything she does to make sure she does it right. No one wants to take action on this issue except me and I can't do anything other then document it and tell her every time. And of course since no one else is doing anything about it I am the bad guy for "telling on her". The pathologists agree with me that something needs done and they all have told the manager to do something. But they don't want to get involved because "personal issues are not thier issues". I don't know what else to do. __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From studentofdeath <@t> hotmail.com Sun May 14 16:16:39 2006 From: studentofdeath <@t> hotmail.com (Amy Senn) Date: Sun May 14 16:16:44 2006 Subject: [Histonet] Lisa, histotech/grosser Message-ID: Message: 2 Date: Sat, 13 May 2006 14:39:30 -0400 From: "Lisa Travis" Subject: [Histonet] Grossing Techs To: Message-ID: <005d01c676bd$b40f0ea0$030aa8c0@lisascomputer> Content-Type: text/plain; charset="iso-8859-1" Can anyone tell me if there is a set pay scale for HTs that do some of the grossing? Lisa---My ID badge says "histo tech", but I do all the duties of a grossing assistant(?). I haven't touched an embedder or microtome since I started working here, but my pay is comparable to a histo tech salary. So, I'm sorry I can'thelp you...but you did bring up an interesting question for me. Am I making what I should be??...or more...or less? Thanks everyone... Quote of the Week: "That money was for BILLS, you know, like pool." --Ray _________________________________________________________________ Is your PC infected? Get a FREE online computer virus scan from McAfee® Security. http://clinic.mcafee.com/clinic/ibuy/campaign.asp?cid=3963 From AnthonyH <@t> chw.edu.au Sun May 14 18:23:53 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun May 14 18:24:12 2006 Subject: [Histonet] Formalin vs. Saline Specimen Submission Message-ID: Vicki, Beware of placing specimens in saline. It is notorious for inducing frozen section artefact in cryotomy (esp Brain and muscle biopsies). Normal Saline, despite its name is not isotonic with cells. Inoshita and Youngberg (Am J Clin Pathol 80(2):206-9, 1983) described the adverse effect on skin biopsies of storage in saline solution. An artefact, consisting of hydropic degeneration of the basal cells and subepidermal bulla formation occurred in skin-punch biopsy specimens immersed in normal saline. They noted similarities between the histologic and ultrastructural findings of this artefact and of epidermolysis bullosa simplex and pathologic hydropic degeneration, as is seen in lupus erythematosus. Now Susan, My responses are below in red Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vicki Gauch Sent: Saturday, 13 May 2006 2:46 AM To: sobrien@bthosp.com; histonet@pathology.swmed.edu Subject: Re: [Histonet] Formalin vs. Saline Specimen Submission Our frozen section room is located in the OR so we receive all of our specimens fresh. The Histotech assigned to the FS room is the one responsible for placing specimens in formalin (or other appropriate fixative for special testing) except after hours. The OR staff then places the specimens in formalin if FS room staff has gone for the day. Any question on tissue for special studies the OR staff pages the tech on call or pathologist on call for direction. We do have a grossing station that has ventilation in case we need it . For specimens submitted in saline....if during the day, the resident assigned to the service for which that specimen was intended is paged immediately for triaging of the case. If after hours, the specimen would most likely be placed in the refrigerator overnight and picked up first thing in the morning. Generally, if the specimen is to be handled right away they generally put enough saline on the tissue to keep it wet. If it is going to sit longer than that, more saline would be used. Specimens that need to be refrigerated for any reason are stored in the grossing room refrigerator or Morgue cooler (if too large for the fridge) and after hours,in the fridge in our lab receiving area. After hours, I mentioned how the OR handles their specimens for us...if there are frozen sections involved, the pathologist on call would be paged to come in and handle the case. If the specimen in question comes from an outpatient source, our lab client service center would call the tech on call to see how to handle fresh or saline soaked specimens. We do have them refrigerate saline soaked specimens overnight depending on the testing to be performed. Phew...that was a long one !!! Hope it helps.. Vicki Gauch AMCH >>> "O'Brien, Sue" 5/11/2006 12:51:33 PM >>> I was wondering how other hospitals handled specimens submitted from the OR (Operating Room). Currently, our routine specimens (ones that do not require special testing and submission) are submitted in 10% NBF (Neutral Buffered Formalin). Thank-you for taking the time to respond! How are routine specimens submitted to your lab from the OR (chose one)? In 10% neutral buffered formalin a. in formalin (e.g. 10% NBF) b. in saline c. fresh d. other (specify): A. For specimens are received by OR in formalin: Is the specimen placed in formalin in the OR procedure room? Yes If yes, how is it done? a. under a fume hood b. placed in container which has formalin - yes c. other (specify): If no, how is it done? a. specimen placed in container and taken fresh to another room where formalin is added b. other (specify): A mix of both above (big specimens have formalin added, small biopsies placed in formalin in theatres) Does the OR have a fume hood available for use (to put specimens into formalin under it)? B. For specimens submitted by OR in saline: NEVER a. How long (generally) is the specimen in saline until it is placed into formalin? b. What volume of saline is used? (e.g. enough to keep moist, or enough to submerge specimen)? c. Who transfers the specimen into formalin? (e.g. histo or lab tech at time of receipt, or does it wait until the Pathologist grosses it? If it waits for the Pathologist, then for how long, and how is it refrigerated?) d. Are specimens refrigerated? If yes, where (e.g. in OR, in lab, in histology). C. For specimens submitted fresh: a. how long (generally) is the specimen fresh before placement into formalin? Depends on tests requested, some delivered directly to Histopath on saline dampened gauge b. How do you keep the small specimens from drying out? In a good quality sealed container, biopsies should not dry out in the short time it takes for the specimen to reach the lab. c. Who transfers the specimen into formalin? (e.g. histo or tech at time of receipt, or does it wait until the Pathologist grosses it? If it waits for the Pathologist, then for how long, and how is it refrigerated?) Depends on test requested. d. Are specimens refrigerated? If yes, where (e.g. in OR, in lab, in histology). If you receive specimens either in saline or fresh (and they are not refrigerated immediately): a. How do you feel they compare to specimens that were immediately placed into formalin? (e.g. better morphology, worse morphology, etc). Comparable morphology as long as saline not used (see above) b. Who handles specimens submitted after hours? (e.g. weekends/holidays; are they left at room temp, refrigerated, or does someone (who?) transfer them into formalin (how?). Always placed in formalin, otherwise scientist on call attends to the specimen. Sorry for the length, but I would really appreciate knowing how others handle this. Sincerely, Susan O'Brien, Histology Supervisor Burdette Tomlin Memorial Hospital Cape May Court House, NJ 08210 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From jfern <@t> unimelb.edu.au Sun May 14 20:56:54 2006 From: jfern <@t> unimelb.edu.au (John Fernandez) Date: Sun May 14 20:57:12 2006 Subject: [Histonet] Re: Golgi stain question/Rapid Golgi Stain (FD Neurotech) In-Reply-To: <446354D3.D15CBAB3@uwo.ca> References: <1706.128.250.186.193.1147241410.squirrel@webmail.unimelb.edu.au> <446354D3.D15CBAB3@uwo.ca> Message-ID: <2338.128.250.186.193.1147658214.squirrel@webmail.unimelb.edu.au> John, thanks for your reply; the kit contents are of the Cox'x variety. That is, solution A of the "FD Rapid GolgiStain Kit" contains Potassium Dichromate + Mercuric Chloride. Solution A is added to solution B (Potassium Chromate) and the brain tissue is left to be impregnated with this mixture for two weeks after which it is transferred to a sucrose solution for 4 days before being cut and slide mounted and put through further staining with solutions D & E (contents of which the company has not disclosed). With the Cox's method that you know of, is there a fixation step (other than dehydrating in ethanol gradient and xylene) that is undertaken to stop the reaction? As I have mentioned we do get excellent initial staining but the problem is that it continues on even after coverslipping. Thanks, John F > The first thing you need to find out is whst's in > the kit. > > Is the method the one traditionally known as the > rapid Golgi method? This involves fixation in an > osmium tetroxide-potassium dichromate mixture, for > 2 to 7 days (the time affects the outcome, but > unpredictably) followed by immersion in aqueous > silver nitrate for a few days, and preparing thick > celloidin or paraffin sections. There are plenty > of later variants, having in common a silver > chromate end product, but these should not be > called "the rapid Golgi method". The sections are > mounted in thick Canada balsam (the real stuff) > without coverslips. If a coverslip is applied, the > preparations fade with time. There are various > chemical tricks to prevent such fading. > > The other major member of this group of methods is > Cox's, with mercuric chloride and potassium > dichromate, but no silver. The dark deposit in > this case is thought to be a mixture of mercurous > oxide and colloidal mercury. These methods are > often called Golgi-Cox, even though Golgi didn't > invent or use them. Ideally Golgi-Cox preparations > should also be mounted without coverslips, but > they will keep for a few years in DPX with > coverslips. > > John Kiernan > Anatomy, UWO > London, Canada > ____________________ > John Fernandez wrote: >> >> Hi all, >> >> our lab has recently purchased and used the Rapid Golgi Stain Kit from >> FD >> Neurotech, resulting in very impressive staining down to dendritic spine >> level, however as Julie Heinrich found, within 24 hours this staining >> becomes very granular with loss of distinct morphology that was >> otherwise >> seen previously. It progressively worsens over time. >> >> Has anyone successfully used the FD Neurotech Rapid Golgi kit? Or does >> anyone know of a mounting medium that can be used to adequately stop the >> reaction that is taking place? Any ideas on what could be causing this >> progressive deterioration of staining would be very much appreciated. >> >> Thanks in advance, >> >> John >> >> John Fernandez >> Research Assistant >> Department of Medicine >> University of Melbourne >> Austin & Repatriation Medical Centre >> Heidelberg, VIC 3084 >> Ph: (03) 9496 3257 >> >> Re: Golgi stain question >> From: "J. A. Kiernan" >> >> -------------------------------------------------------------------------------- >> >> On Fri, 30 Mar 2001, Julie Heinrich wrote: >> >> > I am terribly sorry to bother you- I have been looking through the >> > histonet web page and have found several helpful replies from you >> > about the Gibb & Kolb Golgi stain method. I haven't managed to >> > figure out how to properly post a question there - >> >> You can't. It's a collection of old (archived) communications. >> To ask or answer questions you have to subscribe to the >> listserver. This is done by sending an email to >> histonet@pathology.swmed.edu with the one word subscribe >> in the Subject line. Nothing else. You'll then get an automatic >> reply from the listserver telling you all about it. >> >> > I'm attempting to use the method on avian tissue. I have obtained >> > some decent looking tissue so far (though the stain is a dark >> > tan/brown, rather than the preferable black that I had expected) yet >> > after time the stain turns very 'grainy'. Within a matter of just 24 >> > hours, the dendrites/spines look like collections of dots rather than >> > complete structures, and it gets worse with time. >> >> > Do you have any idea why this might be happening? (I'm following >> > their protocol to the best of my knowledge, and use fresh solutions >> > each time I run tissue) >> >> A graduate student here called Tim Ho did great numbers of Kolb >> Golgis on rat brains a few years ago. He went to Kolb's lab in >> Lethbridge, Alta for guidance. His initial problem was that the >> unstained spaces between the black cells were pale green and >> a bit granular. If I remember rightly, the washing after the >> Golgi-Cox solution needed to be more thorough. >> >> Your problem is different, and may relate to the mounting medium. >> Traditionally the sections were mounted in thick Canada balsam >> without coverslips. A modern synthetic medium + coverslip can >> be followed by fading, but I haven't heard of this happening >> in 24 hours. 6 months, yes. Are you cutting vibratome sections >> of adequate thickness? I think you must be doing something wrong >> at or after the sectioning step, but don't know what. Sorry I >> can't be more helpful. >> >> ---------------------------------------- >> John A. Kiernan >> Department of Anatomy & Cell Biology >> The University of Western Ontario >> London, Canada N6A 5C1 >> kiernan@uwo.ca >> http://publish.uwo.ca/~jkiernan >> >> -------------------------------------------------------------------------------- >> >> << Previous Message | Next Message >> >> -- >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- John Fernandez Research Assistant Department of Medicine University of Melbourne Austin & Repatriation Medical Centre Heidelberg, VIC 3084 Ph: (03) 9496 3257 From kwuny <@t> email.cs.nsw.gov.au Sun May 14 21:27:26 2006 From: kwuny <@t> email.cs.nsw.gov.au (Young Kwun) Date: Sun May 14 21:22:56 2006 Subject: [Histonet] Zeus scientific company Message-ID: <200605151222765.SM01536@crgcsls814> Hello All, Has anyone tried to order from a company called Zeus Scientific? They never replied to any enquiries so far and no company in Australia seemed to distribute their products. We are interested in buying some ready made Michel's Transport and Rinse buffers instead of making up all the time. Does anyone know the other source for these buffers? Thanks, Young From laurie.reilly <@t> jcu.edu.au Sun May 14 21:26:21 2006 From: laurie.reilly <@t> jcu.edu.au (Laurie Reilly) Date: Sun May 14 21:25:29 2006 Subject: [Histonet] Blocking endogenous peroxidase in pig LN Message-ID: <5.2.0.9.0.20060515121712.00bd66c8@mail.jcu.edu.au> Histonetters, Has anyone had experience with IHC on pig (hog) tissues? We are having trouble trying to block endogenous peroxidase in eosinophils in frozen sections of lymph nodes. We have tried hydrogen peroxide up to 6% for one hour. Periodic acid at 0.23% for 1 minute helped but didn't remove all the staining, longer time (up to 8 minutes) didn't improve the removal. Any help would be greatly appreciated. Regards, Laurie. Mr.Laurie Reilly School of Veterinary and Biomedical Sciences James Cook University Townsville. 4811 Australia. Phone 07 4781 4468 Fax 07 4779 1526 From myra047 <@t> yahoo.com Sun May 14 21:41:37 2006 From: myra047 <@t> yahoo.com (myra harris) Date: Sun May 14 21:41:41 2006 Subject: [Histonet] Unsuscribe Message-ID: <20060515024137.6958.qmail@web32104.mail.mud.yahoo.com> I would like for my email address to be taken off of your list server. Thank you. Myra Harris --------------------------------- Love cheap thrills? Enjoy PC-to-Phone calls to 30+ countries for just 2?/min with Yahoo! Messenger with Voice. From kemlo.rogerson <@t> waht.swest.nhs.uk Mon May 15 02:19:28 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon May 15 02:19:12 2006 Subject: [Histonet] Help! Message-ID: I think Luis may have a point; does she have dyscalculia? But either way that may make her unsuitable for the job. I think it is important you document the errors and also document your conversations with her. I would by now have sent her Occupational Health to see if there is a problem processing numbers and/ or any other medical problems. You will need to engage her Union, if she has one, then ultimately you will find yourself at the final disciplinary hearing and sacking. The only other thing you can try is redeployment to another job that doesn't require the use of numbers; failing that it's cheaper to manage a dismissal and any Employment Tribunal fallout than litigation if something goes wrong because of a problem you knew about but never sorted; Managers sometimes have to make the difficult decision. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 The real does not die, the unreal never lived. --Nisargadatta Maharaj This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From kemlo.rogerson <@t> waht.swest.nhs.uk Mon May 15 02:20:31 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon May 15 02:20:15 2006 Subject: [Histonet] Help! Message-ID: With respect that was just words; what would you do? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 The real does not die, the unreal never lived. --Nisargadatta Maharaj This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Janet.Bonner <@t> FLHOSP.ORG Mon May 15 06:46:32 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Mon May 15 06:51:18 2006 Subject: [Histonet] Help! References: Message-ID: We had a very similar problem. After sending her off for a medical check, it was discovered that she was Seriously ill ! Send her to a doctor NOW.. Your first responsibility to your employees is Care and Compassion. If that fails, Is something distracting her (music, conversation, a certain personality of a coworker?) Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kemlo Rogerson Sent: Mon 5/15/2006 3:19 AM To: 'Histo Lady'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Help! I think Luis may have a point; does she have dyscalculia? But either way that may make her unsuitable for the job. I think it is important you document the errors and also document your conversations with her. I would by now have sent her Occupational Health to see if there is a problem processing numbers and/ or any other medical problems. You will need to engage her Union, if she has one, then ultimately you will find yourself at the final disciplinary hearing and sacking. The only other thing you can try is redeployment to another job that doesn't require the use of numbers; failing that it's cheaper to manage a dismissal and any Employment Tribunal fallout than litigation if something goes wrong because of a problem you knew about but never sorted; Managers sometimes have to make the difficult decision. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 The real does not die, the unreal never lived. --Nisargadatta Maharaj This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From aboris <@t> agh.org Mon May 15 06:52:40 2006 From: aboris <@t> agh.org (Anthony F. Boris) Date: Mon May 15 06:53:03 2006 Subject: [Histonet] Help! Message-ID: What you shoul do and what you actually do may be completely different. You should do as the previous emails state and document everything and move forward with your disciplinary steps. The errors will eventually cause a wrong diagnosis being given to a patient and possibly an unneeded surgery or treatment. What I would do is take the tech off any duty involving numbering and just have them do the manual work of routine maintenance like changing the stainer and putting away stock. I then would have to cover the tech with forced overtime and delayed cases. When you have to explain the delay and overtime, maybe something will be done by HR. Good luck with that tough job, Tony -----Original Message----- From: Kemlo Rogerson [mailto:kemlo.rogerson@waht.swest.nhs.uk] Sent: Mon 5/15/2006 3:20 AM To: 'Owen, Michael P'; 'Histonet' Cc: Subject: RE: [Histonet] Help! With respect that was just words; what would you do? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 The real does not die, the unreal never lived. --Nisargadatta Maharaj This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon May 15 07:22:09 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon May 15 07:22:12 2006 Subject: [Histonet] HELP! Message-ID: <20060515122209.90551.qmail@web61216.mail.yahoo.com> This is another example of how frustrating a supervisory position can be. It is not that "glamourous" assignment when the supervisor is worried about what the consequences could be for patient care and is caught between complaining /demanding pathologists and inactive/complacent upper management and HR department when dealing with a histotech that seems unable to correct a mistaken behaviour. You have done your documentation of all the mistakes, the pathologists want something to be done and now the only way you have to try to solve this problem (and help your histotech in case there is a real subyacent disability behing it all) is to concentrate your efforts with HR and it would be advisable to get involved the legal dpt. as well, because they will have some explaining to do in case a malpractice is ever filed as results of a mistake done by this histotech. Ruling out the posibility of any intentional behaviour, this histotech seems that cannot honestly prevent the mistakes, so the responsibility to solve this situation is now with upper management, including the Medical Director of the laboratory. Concentrate in upper management to solve this problem because at this level all the required steps to try to solve it are out of your decision level. Hope this will help you! Ren? J. --------------------------------- Yahoo! Mail goes everywhere you do. Get it on your phone. From histology <@t> gradymem.org Mon May 15 08:03:41 2006 From: histology <@t> gradymem.org (histology@gradymem.org) Date: Mon May 15 08:09:08 2006 Subject: [Histonet] Help! Message-ID: Don't you have a Risk Management department that would be very interested in the fact that they have not done something about this mislabel-er? You may have to go "over the head" of your manager and HR department to assure patient care. Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org ----- Original Message ----- From: Histo Lady Date: Friday, May 12, 2006 4:38 pm Subject: [Histonet] Help! > I don't know what else to do. I am the pathology supervisor and > have a histo tech that continuously mislabels cassettes and > slides. Fortunately we have been able to fix everything, so far. > Yeah I know at some point we won't be able to. This has been > going on for sometime. Each time I tell her that she mislabeled > something and each time I tell my manager and document it. The > manager and HR have spoken to her on 3 different occasions. Yes I > said 3 and she has mislabeled cassettes and/or slides more times > then that. And yet she still has a job. I have tried everything > short of sitting behind her all day and watch everything she does > to make sure she does it right. No one wants to take action on > this issue except me and I can't do anything other then document > it and tell her every time. And of course since no one else is > doing anything about it I am the bad guy for "telling on her". > The pathologists agree with me that something needs done and they > all have told the > manager to do something. But they don't want to get involved > because "personal issues are not thier issues". I don't know what > else to do. > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From nellisk <@t> mail.nih.gov Mon May 15 08:47:01 2006 From: nellisk <@t> mail.nih.gov (Nellis, Kevin Lee (NIH/NCI) [E]) Date: Mon May 15 08:47:09 2006 Subject: [Histonet] Histology Supervisory vacancy for the NCI Message-ID: Dear All: We are recruiting for a Histology Supervisor for the Laboratory of Pathology at the National Cancer Institute. Please forward this announcement to anyone that might be interested. This position closes on Friday, May 19. GS-10, Salary range $ 49,397 - $ 64,213 GS-11, Salary range $ 54,272 - $ 70,558 GS-12, Salary range $ 65,048 - $ 84,559 Recruitment bonus and relocation expenses may be paid for this opportunity. See job posting for examples of Specialized Experience for each salary level. Be sure to follow instructions and answer the questions to support the KSA's specified in the announcement. To review information about this opportunity and apply for the job, please visit: http://jobsearch.usajobs.opm.gov/getjob.asp?JobID=43013007&WT.mc_n=MKT00 0125 If you have trouble with the link, go to www.USAjobs.gov and enter "NCI-06-116352A" in the keyword search field. NOTE: Due to a clarification in qualifications requirements, the Histology Supervisory vacancy for the NCI Laboratory of Pathology has been reposted. If you have applied in the past, your application will be considered again for this vacancy; therefore, you do not need to apply. However, if your experience and/or education have changed since you last applied for this vacancy, you are encouraged to reapply through USA Jobs. Sincerely, Kevin L. Nellis, M.S., M.T. (A.S.C.P.) Clinical Laboratory Manager Laboratory of Pathology Center for Cancer Research National Cancer Institute National Institutes of Health U.S. Department of Health and Human Services 10 Center Drive, Room 2A33, MSC 1500 Bethesda, MD 20892 OFFICE: (301) 594-9532 FAX: (301) 402-0043 http://home.ncifcrf.gov/ccr/lop/Clinical/default.asp From Barry.R.Rittman <@t> uth.tmc.edu Mon May 15 08:47:44 2006 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Mon May 15 08:47:52 2006 Subject: [Histonet] Help! Message-ID: Sounds as if you have done your best to remedy the situation and to also document appropriate mislabeling. Upper management is often reluctant to act, sometimes because of fear of lawsuits related to gender or ethnicity. I think, as suggested earlier, that you formally request your supervisor to terminate this individual as I believe this is what you need to do. If they will not do this then your only other recourse is to list some of the potential problems (lawsuits etc) that could arise because of these mistakes such as loss of future business and also the individuals who would be most likely to be sued. I would also look at some lawsuits last year that resulted in substantial damages to institutions with similar business. Nothing activates top administrators more than potential loss of funds and the possibility of other lawsuits that could also arise. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histology@gradymem.org Sent: Monday, May 15, 2006 8:04 AM To: Histo Lady Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Help! Don't you have a Risk Management department that would be very interested in the fact that they have not done something about this mislabel-er? You may have to go "over the head" of your manager and HR department to assure patient care. Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org ----- Original Message ----- From: Histo Lady Date: Friday, May 12, 2006 4:38 pm Subject: [Histonet] Help! > I don't know what else to do. I am the pathology supervisor and > have a histo tech that continuously mislabels cassettes and > slides. Fortunately we have been able to fix everything, so far. > Yeah I know at some point we won't be able to. This has been > going on for sometime. Each time I tell her that she mislabeled > something and each time I tell my manager and document it. The > manager and HR have spoken to her on 3 different occasions. Yes I > said 3 and she has mislabeled cassettes and/or slides more times > then that. And yet she still has a job. I have tried everything > short of sitting behind her all day and watch everything she does > to make sure she does it right. No one wants to take action on > this issue except me and I can't do anything other then document > it and tell her every time. And of course since no one else is > doing anything about it I am the bad guy for "telling on her". > The pathologists agree with me that something needs done and they > all have told the > manager to do something. But they don't want to get involved > because "personal issues are not thier issues". I don't know what > else to do. > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JGREWE <@t> OhioHealth.com Mon May 15 08:50:12 2006 From: JGREWE <@t> OhioHealth.com (JGREWE@OhioHealth.com) Date: Mon May 15 08:50:27 2006 Subject: [Histonet] Biogenex stainer for special stains Message-ID: Does anyone use any of the Biogenex stainers for their special stains? I would like some feedback as to their ease of use, quality of stain, and reliability. Thanks From pmarcum <@t> vet.upenn.edu Mon May 15 09:00:19 2006 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Mon May 15 09:00:31 2006 Subject: [Histonet] Help! In-Reply-To: References: Message-ID: <6.1.1.1.2.20060515095751.0196f080@mail.vet.upenn.edu> Barry is right and if there is no medical issue it must be dealt with in a fair way for the patients and the institution. Sometimes money issues are the only thing that will activate management to move!! Pam Marcum At 09:47 AM 5/15/2006, Rittman, Barry R wrote: >Sounds as if you have done your best to remedy the situation and to also >document appropriate mislabeling. >Upper management is often reluctant to act, sometimes because of fear of >lawsuits related to gender or ethnicity. >I think, as suggested earlier, that you formally request your supervisor >to terminate this individual as I believe this is what you need to do. >If they will not do this then your only other recourse is to list some >of the potential problems (lawsuits etc) that could arise because of >these mistakes such as loss of future business and also the individuals >who would be most likely to be sued. >I would also look at some lawsuits last year that resulted in >substantial damages to institutions with similar business. Nothing >activates top administrators more than potential loss of funds and the >possibility of other lawsuits that could also arise. >Barry > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >histology@gradymem.org >Sent: Monday, May 15, 2006 8:04 AM >To: Histo Lady >Cc: histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] Help! > > > Don't you have a Risk Management department that would be >very > interested in the fact that they have not done something about >this > mislabel-er? You may have to go "over the head" of your manager >and > HR department to assure patient care. > Angie Barnett, HTL(ASCP) > Grady Memorial Hospital > Pathology Department > 405/224-2258 > histology@gradymem.org > > ----- Original Message ----- > > From: Histo Lady > > Date: Friday, May 12, 2006 4:38 pm > > Subject: [Histonet] Help! > > > I don't know what else to do. I am the pathology supervisor and > > have a histo tech that continuously mislabels cassettes and > > slides. Fortunately we have been able to fix everything, so far. > > Yeah I know at some point we won't be able to. This has been > > going on for sometime. Each time I tell her that she mislabeled > > something and each time I tell my manager and document it. The > > manager and HR have spoken to her on 3 different occasions. Yes I > > said 3 and she has mislabeled cassettes and/or slides more times > > then that. And yet she still has a job. I have tried everything > > short of sitting behind her all day and watch everything she does > > to make sure she does it right. No one wants to take action on > > this issue except me and I can't do anything other then document > > it and tell her every time. And of course since no one else is > > doing anything about it I am the bad guy for "telling on her". > > The pathologists agree with me that something needs done and they > > all have told the > > manager to do something. But they don't want to get involved > > because "personal issues are not thier issues". I don't know what > > else to do. > > __________________________________________________ > > Do You Yahoo!? > > Tired of spam? Yahoo! Mail has the best spam protection around > > http://mail.yahoo.com > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From VAZQUEZR <@t> ohsu.edu Mon May 15 09:04:22 2006 From: VAZQUEZR <@t> ohsu.edu (Robyn Vazquez) Date: Mon May 15 09:04:59 2006 Subject: [Histonet] Zeus scientific company Message-ID: Try getting it through Wampole Labs. They are a distributor of Zeus Sci 1-609-627-8000 Robyn OHSU >>> "Young Kwun" 5/14/2006 7:27 PM >>> Hello All, Has anyone tried to order from a company called Zeus Scientific? They never replied to any enquiries so far and no company in Australia seemed to distribute their products. We are interested in buying some ready made Michel's Transport and Rinse buffers instead of making up all the time. Does anyone know the other source for these buffers? Thanks, Young _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LRaff <@t> lab.uropartners.com Mon May 15 09:05:30 2006 From: LRaff <@t> lab.uropartners.com (Lester Raff) Date: Mon May 15 09:05:36 2006 Subject: [Histonet] Stainers Message-ID: <5DA1CA5D0B98A84985B545A24423B8220197AA@UPLAB01.uplab.local> Do any of you histonetters share your automated stainers with cytology? If so, how does it work out? Thanks, Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 From histolog <@t> fcv.unl.edu.ar Mon May 15 09:18:34 2006 From: histolog <@t> fcv.unl.edu.ar (Lab. Invest. Histol.) Date: Mon May 15 09:18:34 2006 Subject: [Histonet] In situ hybridization to y-chromosome Message-ID: Has anyone had experience with in situ hybridization to genomic DNA (y-chromosome) in paraffin-embedded sections of formol-fixed tissue material?? Any help (including protocols) would be greatly appreciated. Thanks!! ------------------------------------------------------------------- Dr. Hugo H. Ortega (DMV, PhD) Departament of Cellular Biology Faculty of Veterinary Sciences Universidad Nacional del Litoral R.P. Kreder 2805 - Esperanza (3080) Santa Fe - ARGENTINA From gcallis <@t> montana.edu Mon May 15 09:18:28 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon May 15 09:18:44 2006 Subject: [Histonet] Technovit 9100 New and Technovit 4000 In-Reply-To: <1147468508.4464fadc1fd40@www.inbox.lv> References: <1147468508.4464fadc1fd40@www.inbox.lv> Message-ID: <6.0.0.22.1.20060515081600.01b4d3b8@gemini.msu.montana.edu> Superglue may work even better, it sets up fast. I have sent this message to a lady who works with EKAKT, she should reply to you unless she is looking in on Histonet. At 03:15 PM 5/12/2006, you wrote: >Hello, > >In our lab, we are working with EXAKT Cutting/Grinding system. For >tissue embedding and polymerization we are going to use Technovit >9100 New embedding medium. We have never done it before! To attach >the plastic block to a slide we are going to use Technovit 4000. Will >it be ok to use Technovit 4000 in this case? Simply, before we used >other materials… > >Thank you very much! > >Best regards, >Andrey Ivanov >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From mauger <@t> email.chop.edu Mon May 15 09:35:31 2006 From: mauger <@t> email.chop.edu (Joanne Mauger) Date: Mon May 15 09:36:28 2006 Subject: [Histonet] aspergillus antibody Message-ID: Hello histonetters, Is anyone aware of a monoclonal antibody to aspergillus? I have been using Dako's clone WF-AF-1, but they are discontinuing it -along with many others! I found several polyclonals, but not another mono. If anyone has a source, please share. Thanks, Jo >>> "C.M. van der Loos" 05/12/06 2:42 AM >>> Dear Richard, In collaboration with LabVision I have put the lectures that are part of my multiple staining course on-line: [1]www.labvision.com/indextutorial.cfm. You need to login and then the 1st lecture becomes available. After doing a test the 2nd lecture is available, etc. At this moment there are two tutorials on-line. That will be expanded soon to 6 tutorials all dealing with multiple staining issues. Have fun with it! Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Thu, 11 May 2006 11:47:19 +0100 From: "Edwards, R.E." Subject: [Histonet] double immunostaining To: "histonet" Information please on the preferred kits/systems used for double/triple immunostaining(non-fluorescence), probably using combinations of anti-mouse and rabbit primaries, on paraffin sections. Many thanks &nbs! p; & References 1. http://www.labvision.com/indextutorial.cfm _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.m.vanderloos <@t> amc.uva.nl Mon May 15 09:44:48 2006 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Mon May 15 09:45:00 2006 Subject: [Histonet] Re: aspergillus antibody Message-ID: Dear Joanne, Have a look at Biogenesis ([1]http://www.biogenesis.co.uk/catalogue). There is an aspergillus mouse (IgM) antibody in their catalogue, unlabeled, biotinylated, FITC-conjugated, etc. Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands ----- Original Message ----- From: Joanne Mauger Date: Monday, May 15, 2006 4:35 pm Subject: aspergillus antibody > Hello histonetters, > > Is anyone aware of a monoclonal antibody to aspergillus? I have been > using Dako's clone WF-AF-1, but they are discontinuing it -along with > many others! I found several polyclonals, but not another mono. If > anyone has a source, please share. > > Thanks, References 1. http://www.biogenesis.co.uk/catalogue From gcallis <@t> montana.edu Mon May 15 09:45:37 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon May 15 09:45:48 2006 Subject: [Histonet] Blocking endogenous peroxidase in pig LN In-Reply-To: <5.2.0.9.0.20060515121712.00bd66c8@mail.jcu.edu.au> References: <5.2.0.9.0.20060515121712.00bd66c8@mail.jcu.edu.au> Message-ID: <6.0.0.22.1.20060515084238.01b90e10@gemini.msu.montana.edu> I have a peroxidase and pseudoperoxidase block that uses Glucose oxidase for complete removal of these endogenous problems!!! It was originally used for an eosinophil study so I will attach to you privately. We refer to it as GLUOX Block. The method comes with references, you may want to access and read. It works for minimally fixed frozen sections (acetone fixed) and also on FFPE tissues, and will not chew the frozen sections off the slide. At 08:26 PM 5/14/2006, you wrote: >Histonetters, >Has anyone had experience with IHC on pig (hog) tissues? >We are having trouble trying to block endogenous peroxidase in eosinophils >in frozen sections of lymph nodes. >We have tried hydrogen peroxide up to 6% for one hour. >Periodic acid at 0.23% for 1 minute helped but didn't remove all the >staining, longer time (up to 8 minutes) didn't improve the removal. > >Any help would be greatly appreciated. > > Regards, Laurie. > > > >Mr.Laurie Reilly >School of Veterinary and Biomedical Sciences >James Cook University >Townsville. 4811 >Australia. > >Phone 07 4781 4468 >Fax 07 4779 1526 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From gcallis <@t> montana.edu Mon May 15 10:05:01 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon May 15 10:05:04 2006 Subject: supplier Re: [Histonet] aspergillus antibody In-Reply-To: References: Message-ID: <6.0.0.22.1.20060515085155.01baccb8@gemini.msu.montana.edu> Try Biodesign International, in Maine, who carry Aspergillus monoclonal made in mouse but it is an IgM not IgG antibody. Make sure you secondary is detecting mouse IgM. Call 888-530-0140 or www.biodesign.com Interestingly they have a very large array of infectious organism antibodies. At 08:35 AM 5/15/2006, you wrote: >Hello histonetters, > >Is anyone aware of a monoclonal antibody to aspergillus? I have been >using Dako's clone WF-AF-1, but they are discontinuing it -along with >many others! I found several polyclonals, but not another mono. If >anyone has a source, please share. > >Thanks, > >Jo > > >>> "C.M. van der Loos" 05/12/06 2:42 AM > >>> > > Dear Richard, > > In collaboration with LabVision I have put the lectures that are >part > of my multiple staining course on-line: > [1]www.labvision.com/indextutorial.cfm. You need to login and then >the > 1st lecture becomes available. After doing a test the 2nd lecture >is > available, etc. At this moment there are two tutorials on-line. >That > will be expanded soon to 6 tutorials all dealing with >multiple > staining issues. Have fun with it! > > Chris van der Loos, PhD > Dept. of Pathology > Academic Medical Center M2-230 > Meibergdreef 9 > NL-1105 AZ Amsterdam > The Netherlands > > > Date: Thu, 11 May 2006 11:47:19 +0100 > From: "Edwards, R.E." > Subject: [Histonet] double immunostaining > To: "histonet" > Information please on the preferred kits/systems used >for > double/triple immunostaining(non-fluorescence), probably >using > combinations of anti-mouse and rabbit primaries, on >paraffin > sections. > > Many thanks > &nbs! p; & > >References > > 1. http://www.labvision.com/indextutorial.cfm >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From TJJ <@t> Stowers-Institute.org Mon May 15 10:27:31 2006 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Mon May 15 10:27:59 2006 Subject: [Histonet] Loss of immunoreactivity in cryosections Message-ID: Has anyone experienced a loss of immunoreactivity in cryosections that have airdried overnight prior to staining or storage at -80 degrees C? And if so, are these unfixed cryosections, or fixed, cryoprotected cryosections? Thank you for any information you can provide, the more info, the better. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64133 From jkiernan <@t> uwo.ca Mon May 15 10:59:58 2006 From: jkiernan <@t> uwo.ca (John A. Kiernan) Date: Mon May 15 10:59:43 2006 Subject: [Histonet] Re: Golgi stain question/Rapid Golgi Stain (FDNeurotech) References: <1706.128.250.186.193.1147241410.squirrel@webmail.unimelb.edu.au> <446354D3.D15CBAB3@uwo.ca> <2338.128.250.186.193.1147658214.squirrel@webmail.unimelb.edu.au> Message-ID: <4468A57E.FA5006AB@uwo.ca> Your kit's literature declares itself (partly). It is neither rapid nor Golgi, and certainly not the classical rapid Golgi method! It is evidently a "Golgi-Cox" method, perhaps based on Sholl's modification or one of Kolb's much more recently published variants. These are used for looking at dendrites (branching patterns, spines). The secret items C & D in the kit will be for the alkaline reduction step that deposits the black stuff. In published methods this is usually a 1:20 dilution of ammonium hydroxide (.880 ammonia) in water. A kit could generate an equivalent solution by mixing two components (such as any ammonium salt and any stronger base - KOH, NaOH, Na2CO3) in a prescribed volume of water. The calculation is an elementary one. To answer your question about fixation: The mercuric chloride/dichromate/chromate mixture serves as the fixative in methods of this type. You say that the kit advises moving the blocks into sucrose; presumably this is for cutting frozen sections. But you also talk about dehydrating in alcohol to "stop the reaction". Reaction-stopping with alcohol isn't part of any Golgi or Cox method. With any histological procedure involving dichromate as a fixative or other agent, thorough washing in water (hours; slowly running, or plenty of changes) is necessary to remove unbound dichromate before going into alcohol, with which there is a chemical reaction that leads to precipitation of Cr2O3. John Kiernan Anatomy, UWO London, Canada. _____________________________________- John Fernandez wrote: > > John, thanks for your reply; > > the kit contents are of the Cox'x variety. > That is, solution A of the "FD Rapid GolgiStain Kit" contains Potassium > Dichromate + Mercuric Chloride. > > Solution A is added to solution B (Potassium Chromate) and the brain > tissue is left to be impregnated with this mixture for two weeks after > which it is transferred to a sucrose solution for 4 days before being cut > and slide mounted and put through further staining with solutions D & E > (contents of which the company has not disclosed). > > With the Cox's method that you know of, is there a fixation step (other > than dehydrating in ethanol gradient and xylene) that is undertaken to > stop the reaction? > > As I have mentioned we do get excellent initial staining but the problem > is that it continues on even after coverslipping. > > Thanks, > John F > > > The first thing you need to find out is whst's in > > the kit. > > > > Is the method the one traditionally known as the > > rapid Golgi method? This involves fixation in an > > osmium tetroxide-potassium dichromate mixture, for > > 2 to 7 days (the time affects the outcome, but > > unpredictably) followed by immersion in aqueous > > silver nitrate for a few days, and preparing thick > > celloidin or paraffin sections. There are plenty > > of later variants, having in common a silver > > chromate end product, but these should not be > > called "the rapid Golgi method". The sections are > > mounted in thick Canada balsam (the real stuff) > > without coverslips. If a coverslip is applied, the > > preparations fade with time. There are various > > chemical tricks to prevent such fading. > > > > The other major member of this group of methods is > > Cox's, with mercuric chloride and potassium > > dichromate, but no silver. The dark deposit in > > this case is thought to be a mixture of mercurous > > oxide and colloidal mercury. These methods are > > often called Golgi-Cox, even though Golgi didn't > > invent or use them. Ideally Golgi-Cox preparations > > should also be mounted without coverslips, but > > they will keep for a few years in DPX with > > coverslips. > > > > John Kiernan > > Anatomy, UWO > > London, Canada > > ____________________ > > John Fernandez wrote: > >> > >> Hi all, > >> > >> our lab has recently purchased and used the Rapid Golgi Stain Kit from > >> FD > >> Neurotech, resulting in very impressive staining down to dendritic spine > >> level, however as Julie Heinrich found, within 24 hours this staining > >> becomes very granular with loss of distinct morphology that was > >> otherwise > >> seen previously. It progressively worsens over time. > >> > >> Has anyone successfully used the FD Neurotech Rapid Golgi kit? Or does > >> anyone know of a mounting medium that can be used to adequately stop the > >> reaction that is taking place? Any ideas on what could be causing this > >> progressive deterioration of staining would be very much appreciated. > >> > >> Thanks in advance, > >> > >> John > >> > >> John Fernandez > >> Research Assistant > >> Department of Medicine > >> University of Melbourne > >> Austin & Repatriation Medical Centre > >> Heidelberg, VIC 3084 > >> Ph: (03) 9496 3257 > >> > >> Re: Golgi stain question > >> From: "J. A. Kiernan" > >> > >> -------------------------------------------------------------------------------- > >> > >> On Fri, 30 Mar 2001, Julie Heinrich wrote: > >> > >> > I am terribly sorry to bother you- I have been looking through the > >> > histonet web page and have found several helpful replies from you > >> > about the Gibb & Kolb Golgi stain method. I haven't managed to > >> > figure out how to properly post a question there - > >> > >> You can't. It's a collection of old (archived) communications. > >> To ask or answer questions you have to subscribe to the > >> listserver. This is done by sending an email to > >> histonet@pathology.swmed.edu with the one word subscribe > >> in the Subject line. Nothing else. You'll then get an automatic > >> reply from the listserver telling you all about it. > >> > >> > I'm attempting to use the method on avian tissue. I have obtained > >> > some decent looking tissue so far (though the stain is a dark > >> > tan/brown, rather than the preferable black that I had expected) yet > >> > after time the stain turns very 'grainy'. Within a matter of just 24 > >> > hours, the dendrites/spines look like collections of dots rather than > >> > complete structures, and it gets worse with time. > >> > >> > Do you have any idea why this might be happening? (I'm following > >> > their protocol to the best of my knowledge, and use fresh solutions > >> > each time I run tissue) > >> > >> A graduate student here called Tim Ho did great numbers of Kolb > >> Golgis on rat brains a few years ago. He went to Kolb's lab in > >> Lethbridge, Alta for guidance. His initial problem was that the > >> unstained spaces between the black cells were pale green and > >> a bit granular. If I remember rightly, the washing after the > >> Golgi-Cox solution needed to be more thorough. > >> > >> Your problem is different, and may relate to the mounting medium. > >> Traditionally the sections were mounted in thick Canada balsam > >> without coverslips. A modern synthetic medium + coverslip can > >> be followed by fading, but I haven't heard of this happening > >> in 24 hours. 6 months, yes. Are you cutting vibratome sections > >> of adequate thickness? I think you must be doing something wrong > >> at or after the sectioning step, but don't know what. Sorry I > >> can't be more helpful. > >> > >> ---------------------------------------- > >> John A. Kiernan > >> Department of Anatomy & Cell Biology > >> The University of Western Ontario > >> London, Canada N6A 5C1 > >> kiernan@uwo.ca > >> http://publish.uwo.ca/~jkiernan > >> > >> -------------------------------------------------------------------------------- > >> > >> << Previous Message | Next Message >> > >> -- > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- > John Fernandez > Research Assistant > Department of Medicine > University of Melbourne > Austin & Repatriation Medical Centre > Heidelberg, VIC 3084 > Ph: (03) 9496 3257 From shive003 <@t> umn.edu Mon May 15 12:11:29 2006 From: shive003 <@t> umn.edu (Jan Shivers) Date: Mon May 15 12:11:36 2006 Subject: [Histonet] Bartonella/cat scratch fever Message-ID: <00c801c67842$9bbb8410$a1065486@auxs.umn.edu> Does anyone know where I could find a positive control paraffin block for Bartonella/cat scratch fever for IHC? I'd be happy to trade control blocks, if there's anything anyone might need in return. Thanks in advance, Jan Shivers UMN VDL From oskaki <@t> morpheus.wustl.edu Mon May 15 13:28:14 2006 From: oskaki <@t> morpheus.wustl.edu (Dale Osborne) Date: Mon May 15 13:28:20 2006 Subject: [Histonet] autophagy Message-ID: <4468C83E.3000506@morpheus.wustl.edu> Has anyone done staining for autophagy? If so, what stain was used? We would want to stain in vivo models as well as in vitro cultured cells. Thanks, Dale Osborne Washington Univ. School of Medicine Anesthesiology Dept. St. Louis, MO From jtrynak <@t> hotmail.com Mon May 15 13:46:31 2006 From: jtrynak <@t> hotmail.com (John Rynak) Date: Mon May 15 13:46:37 2006 Subject: [Histonet] OPENING: Application Support - IHC TN/KY Message-ID: Application Support - IHC TN/KY SciGenium's Client is experiencing tremendous growth within their North American operations. This growth is due to the recent launch of their FDA approved automated histology instrumentation system, and the growing antibody and reagent consumable product line for the histology market. Due to the new launch of these products they are currently looking am Application Support - IHC, in the following regions : Nashville TN / Louisville KY This position provides technical applications support for our next generation range of automated histopathology products. This is a very hands on role where your key tasks will include: · Building an in-depth understanding of our new product technologies and their applications in a diagnostic histopathology environment; · Customer liaison by providing scientific and laboratory expertise for in-field installations, training and trouble-shooting; · Customer support Help-line for remote problem solving; · Designing and performing experiments to investigate and solve tough technical applications problems; and · Preparing problem resolution reports and imparting your findings and knowledge to internal and field based Product Engineers and Technical Managers. To be successful in this role you will possess: · Excellent problem solving and analytical skills · The ability to communicate with front-end users, technical design engineers and pragmatic sales force members. · A tertiary qualification in a relevant science stream (eg. Medical Laboratory Science) · Practical experience in bench-level histopathology, including clinical immunohistochemistry · Demonstrated use of automated scientific instrumentation BS/ MS in Life Sciences and ASCP Certification Interest in using PC based software and maintenance of automated scientific instrumentation will be highly advantageous. In addition, you are extremely detailed and analytically minded with a strong sense of customer focus. Interested parties should forward a resume to resume@scigenium.com Send to : SciGenium PO 380916 Cambridge, MA 02238 From SHARON.OSBORN <@t> SPCORP.COM Mon May 15 14:54:56 2006 From: SHARON.OSBORN <@t> SPCORP.COM (Osborn, Sharon) Date: Mon May 15 14:56:02 2006 Subject: [Histonet] RE: tech mislabeling slides and cassettes Message-ID: <9A919A5D70313A4D9C56A025710874080C72A2@kenmsg40.us.schp.com> Histo-Lady, All of the responses have validity; Do medical evaluation of her; involve risk management, legal, HR, etc. Is there an EAP program should she be experiencing emotional trauma that is severely interfering? Is she a good to great tech in other areas rather than the labeling; are you short-handed? However, this is also a great situation to bring in some automation in the labeling department. With automated cassette and slide labeling, this could be given to a lab assistant or such person to do. Or, the techs rotate through doing it. Slides are made the day before to match the cassettes generated or, with late ones, early the next morning by a tech or lab assistant (if you are not a 24 hour facility). Therefore, the tech does not have to do any manual labeling; only match slides to cassettes at the microtome and cassettes to the grossing sheet. Has this avenue been explored? Many people transpose numbers and figure out ways to compensate for this; however, this tech may be in denial or it is such a severe problem that other means for numbering is best solution. Sharon Osborn DNAX, SP BioPharma ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From Rcartun <@t> harthosp.org Mon May 15 16:20:26 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Mon May 15 16:21:10 2006 Subject: [Histonet] CD117 Message-ID: <4468B85A02000009000AEA56@hcnwgwds01.hh.chs> Has Dako ever sold a mouse monoclonal or a rabbit monoclonal to CD117 (c-Kit)? Their current catalog only lists a rabbit polyclonal. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From rjbuesa <@t> yahoo.com Mon May 15 16:52:58 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon May 15 16:53:01 2006 Subject: [Histonet] CD117 In-Reply-To: <4468B85A02000009000AEA56@hcnwgwds01.hh.chs> Message-ID: <20060515215258.50495.qmail@web61219.mail.yahoo.com> Up to 2002 I used a monoclonal CD117 by Dako (HIER at pH6) Their cat. code used to be M7140 ($ 181/mL) Ren? J. Richard Cartun wrote: Has Dako ever sold a mouse monoclonal or a rabbit monoclonal to CD117 (c-Kit)? Their current catalog only lists a rabbit polyclonal. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- New Yahoo! Messenger with Voice. Call regular phones from your PC and save big. From KPierce <@t> cancer-test.com Mon May 15 18:35:13 2006 From: KPierce <@t> cancer-test.com (Ken Pierce) Date: Mon May 15 18:35:18 2006 Subject: [Histonet] histo tech position Message-ID: <9380B79A09A6DD43884D977256FC120202B28C@fleming.mla.local> Medical Lab Assoc. in Seattle WA is looking for a bench tech for a 6 AM to 2 PM Shift. Will cross train for cytopreparation and IHC staining. Salary negotiable. Ken Pierce at kpierce@cancer-test.com. From marjoh3 <@t> telus.net Mon May 15 18:41:36 2006 From: marjoh3 <@t> telus.net (Marilyn Johnson) Date: Mon May 15 18:41:40 2006 Subject: [Histonet] IHC Staining for Clostridium haemolyticum Message-ID: <001601c67879$1c9dd700$6401a8c0@VALUED20606295> Hi Histonetters, Sure hope someone out there can help me with this one! We are developing a project with our Parasitology Lab to demonstrate this bacterium, hopefully using a monoclonal primary antisera. Please include the source of reagents and staining procedure. Any replies would be greatly appreciated. Thank you in advance. Marilyn Johnson Alberta Agriculture Edmonton, Alberta, Canada. From kaleid11 <@t> yahoo.com Mon May 15 18:44:28 2006 From: kaleid11 <@t> yahoo.com (Adam Perry) Date: Mon May 15 18:44:35 2006 Subject: [Histonet] immunofluorescence amplification... Message-ID: <20060515234428.43328.qmail@web30412.mail.mud.yahoo.com> What are people's impressions about different (i.e. the best) ways to amplify fluorescent signal from immunocytochemistry? I'm working with 30 um rodent brain sections. When I use a biotinylated secondary antibody with avidin-biotin-HRP detection with DAB or NiDAB I get good cellular staining with a primary antibody concentration of 1:1,000. I am now trying to perform double labeling and so have switched to fluorescence...and I don't seem to be getting any specific signal now. I have tried increasing the antibody concentration from 1:1,000 up to 1:100 and still don't see the faintest hint of staining (and the background just gets really bad). I've used the fluorescent antibody to detect other primaries (so the secondary is working in general and my microscope and filters are fine)...I was thinking that maybe my antigen of interest is expressed at low levels, so maybe the amplification with the avidin-biotin procedure is important...are there similar ways to amplify fluorecent signal? I know I can use fluorescent-avidin molecules to label a biotinylated secondary...does this amplify the signal more than a fluorescent labeled secondary alone? Any thoughts or comments would be great...if you need more details about my specific protocol, please ask... thanks in advance, adam --------------------------------- How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. From blue_eyes002 <@t> hotmail.com Mon May 15 19:10:08 2006 From: blue_eyes002 <@t> hotmail.com (Inge Raycroft) Date: Mon May 15 19:10:13 2006 Subject: [Histonet] Copper controls Message-ID: Hello, I am hoping that someone out there can lend a hand. Our histology lab is in dire need of a copper control. Does anyone have an extra block that they can manage to send us?????? Any help would be appreciated. _________________________________________________________________ Designer Mail isn't just fun to send, it's fun to receive. Use special stationery, fonts and colors. http://join.msn.com/?pgmarket=en-ca&page=byoa/prem&xAPID=1994&DI=1034&SU=http://hotmail.com/enca&HL=Market_MSNIS_Taglines Start enjoying all the benefits of MSN® Premium right now and get the first two months FREE*. From lilbullrider00 <@t> yahoo.com Mon May 15 21:35:36 2006 From: lilbullrider00 <@t> yahoo.com (brent hart) Date: Mon May 15 21:35:40 2006 Subject: [Histonet] looking for ventana email Message-ID: <20060516023536.79674.qmail@web53415.mail.yahoo.com> i am looking for Mike Reichenbach, or Debra Flynns email address i have a couple of immuno questions for them... if any one could help that would be great... thanks --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. From kemlo.rogerson <@t> waht.swest.nhs.uk Tue May 16 02:20:52 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue May 16 02:20:52 2006 Subject: [Histonet] RE: tech mislabeling slides and cassettes Message-ID: There is merit in automating the problem away but the inability to process numbers is likely to reappear in some other task that is not immediately apparent; a good 'Tech', BMS, MLA ought to be word and number literate and massaging the work to fit the Staff may not work. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 The real does not die, the unreal never lived. --Nisargadatta Maharaj This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From JGREWE <@t> OhioHealth.com Tue May 16 02:36:32 2006 From: JGREWE <@t> OhioHealth.com (JGREWE@OhioHealth.com) Date: Tue May 16 02:36:48 2006 Subject: [Histonet] Skin Artifact Identification Message-ID: Does anyone know what may be causing this skin artifact? What we have in some of our skin cases is vacuoles within the nuclei of squamous cells of epidermis on permanent sections. We don't see it all of the time. 10% NBF fixation. Thanks From kemlo.rogerson <@t> waht.swest.nhs.uk Tue May 16 03:32:39 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue May 16 03:32:25 2006 Subject: [Histonet] Skin Artifact Identification Message-ID: Vacuoles or bubbles? Terry Marshall is the expert on skin biopsy nuclei and vacuoles/ bubbles. It is IMHO, a product of a 'soft' fixatives that allows subsequent heat (water bath/ oven) to cause the nuclei to 'bubble'. Adequate fixation or fixation with a 'hard' fixative (mercury, zinc) seems to eradicate as does turning down the water bath and oven; but the sections then float off sometimes. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 The real does not die, the unreal never lived. --Nisargadatta Maharaj This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From c.thompson <@t> student.ucc.ie Tue May 16 05:54:40 2006 From: c.thompson <@t> student.ucc.ie (c.thompson@student.ucc.ie) Date: Tue May 16 05:54:49 2006 Subject: [Histonet] (No subject header) Message-ID: <4469af70.361.4f41.746311485@student.ucc.ie> Hi Histonet, A very basic question but I am a complete novice in the world of immunohistochemistry / immunofluorescence and am trying to teach myself the rudiments! At present, I am attempting to visualise NHE3 in rat kidney using immunofluorescence but am encountering a lot of autofluorescence. I just wanted to enquire about possible reasons for / methods of dealing with this. My method is as follows: Once the kidneys have been excised I fix them in 4% paraformaldehyde overnight (4 degrees celsius) and then incubate them in 30% sucrose until they sink. Kidneys are then frozen in isopentane in liquid nitrogen and cryosectioned. Sections rinsed in distilled water 3 X 5 min washes in PBS Incubation in blocking solution (3% normal goat serum, 0.25% Triton X. 96.75% PBS) for 1 hr @ room temp Slides then incubated with primary antibody (1:500 chemicon anti nhe3) in a humidified chamber @ 4 degrees overnight Slides rinsed in distilled water 3 X 5 min washes in PBS Incubation with 1:500 FITC conjugated secondary antibody for 1 hr at room temp slides rinsed and coverslips applied with vectashield, sealed and visualised Apologies for all the detail, just generally unsure of my method... and whether there are more apt methodologies for this? Getting a lot of autofluorescence and finding it quite difficult to visualise the NHE3 at all! Would really appreciate any help at all! Thank you, Claire From c.thompson <@t> student.ucc.ie Tue May 16 06:38:23 2006 From: c.thompson <@t> student.ucc.ie (c.thompson@student.ucc.ie) Date: Tue May 16 06:38:32 2006 Subject: [Histonet] Re: Autofluorescence/Imunofluorescence protocols rat kidney Message-ID: <4469b9af.7c.7cd1.1813511228@student.ucc.ie> Hi Histonet, A very basic question but I am a complete novice in the world of immunohistochemistry / immunofluorescence and am trying to teach myself the rudiments! At present, I am attempting to visualise NHE3 in rat kidney using immunofluorescence but am encountering a lot of autofluorescence. I just wanted to enquire about possible reasons for / methods of dealing with this. My method is as follows: Once the kidneys have been excised I fix them in 4% paraformaldehyde overnight (4 degrees celsius) and then incubate them in 30% sucrose until they sink. Kidneys are then frozen in isopentane in liquid nitrogen and cryosectioned. Sections rinsed in distilled water 3 X 5 min washes in PBS Incubation in blocking solution (3% normal goat serum, 0.25% Triton X. 96.75% PBS) for 1 hr @ room temp Slides then incubated with primary antibody (1:500 chemicon anti nhe3) in a humidified chamber @ 4 degrees overnight Slides rinsed in distilled water 3 X 5 min washes in PBS Incubation with 1:500 FITC conjugated secondary antibody for 1 hr at room temp slides rinsed and coverslips applied with vectashield, sealed and visualised Apologies for all the detail, just generally unsure of my method... and whether there are more apt methodologies for this? Getting a lot of autofluorescence and finding it quite difficult to visualise the NHE3 at all! Would really appreciate any help at all! Thank you, Claire From rjbuesa <@t> yahoo.com Tue May 16 07:45:15 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue May 16 07:45:21 2006 Subject: [Histonet] Skin Artifact Identification In-Reply-To: Message-ID: <20060516124515.17700.qmail@web61211.mail.yahoo.com> Santoianni & Hammami published an article in Jorunal Histotechnol.[13(2):135-136(1990)] where they attribute a nuclear "bubbling" artifact to oven heat-affixing the sections above 60?C before they were completely free from water underneath. I have been able to corroborate their finding. If this makes sense to you try to be absolutely sure that there is no water left underneath your sections before heating them in the oven. Hope this will help you! Ren? J. JGREWE@OhioHealth.com wrote: Does anyone know what may be causing this skin artifact? What we have in some of our skin cases is vacuoles within the nuclei of squamous cells of epidermis on permanent sections. We don't see it all of the time. 10% NBF fixation. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. PC-to-Phone calls for ridiculously low rates. From kemlo.rogerson <@t> waht.swest.nhs.uk Tue May 16 08:06:37 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue May 16 08:06:21 2006 Subject: [Histonet] Skin Artifact Identification Message-ID: Nah, not even close. The water just bubbles into the nuclei? Very clever water that; fluid intelligence. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 The past is never dead, it is not even past. --William Faulkner This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From shive003 <@t> umn.edu Tue May 16 09:14:33 2006 From: shive003 <@t> umn.edu (Jan Shivers) Date: Tue May 16 09:15:53 2006 Subject: [Histonet] looking for used ultramicrotome Message-ID: <003f01c678f3$0e7e1d90$a1065486@auxs.umn.edu> I'm posting this for a colleague who does not subscribe to the Histonet. Please reply directly to Debra Rokusek: rokus002@umn.edu She is looking for a used ultramicrotome for TEM purposes. Does anyone have some suggestions for her (besides eBay)? Thanks very much in advance. Jan Shivers UMN VDL From gcallis <@t> montana.edu Tue May 16 10:31:14 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue May 16 10:31:24 2006 Subject: [Histonet] Re: Autofluorescence/Imunofluorescence protocols rat kidney In-Reply-To: <4469b9af.7c.7cd1.1813511228@student.ucc.ie> References: <4469b9af.7c.7cd1.1813511228@student.ucc.ie> Message-ID: <6.0.0.22.1.20060516092457.01b556f8@gemini.msu.montana.edu> You will get rid of autofluorescence or most of it by not fixing in an aldehyde, this has been discussed recently and many times on Histonet, so be sure to search archives for some explanations. DITC and your autofluorescence are pretty much the same color. If you do snap frozen fresh tissue, and another kind of fixation (acetone, acetone/alcohol) you will have less problems here. OR you can try a different fluorophore, i.e. a rhodamine RRX from Jackson OR a secondary conjugated to ALexa 555. Red color should come through with autofluorescence as the counterstain, so to speak. It is not so important to see the perfect morphology enjoyed by PFA fixation, but your IFA staining may improve greatly. You can use a mounting media with DAPI also, this brings in the nuclei as a blue color - for contrast and general morphology identification/location of antigen in tissue. Molecular Probes also has secondaries conjugated to Alexa fluorophores, very bright, excellent. I have a review of autofluorescence I am attaching to you privately, it is very informative on fixation, and this problem, etc. At 05:38 AM 5/16/2006, you wrote: >Hi Histonet, > >A very basic question but I am a complete novice in the >world of immunohistochemistry / immunofluorescence and am >trying to teach myself the rudiments! > >At present, I am attempting to visualise NHE3 in rat kidney >using immunofluorescence but am encountering a lot of >autofluorescence. I just wanted to enquire about possible >reasons for / methods of dealing with this. > >My method is as follows: > >Once the kidneys have been excised I fix them in 4% >paraformaldehyde overnight (4 degrees celsius) and then >incubate them in 30% sucrose until they sink. Kidneys are >then frozen in isopentane in liquid nitrogen and >cryosectioned. > >Sections rinsed in distilled water >3 X 5 min washes in PBS >Incubation in blocking solution (3% normal goat serum, 0.25% >Triton X. 96.75% PBS) for 1 hr @ room temp >Slides then incubated with primary antibody (1:500 chemicon >anti nhe3) in a humidified chamber @ 4 degrees overnight > >Slides rinsed in distilled water 3 X 5 min washes in PBS >Incubation with 1:500 FITC conjugated secondary antibody for >1 hr at room temp >slides rinsed and coverslips applied with vectashield, >sealed and visualised > >Apologies for all the detail, just generally unsure of my >method... and whether there are more apt methodologies for >this? Getting a lot of autofluorescence and finding it quite >difficult to visualise the NHE3 at all! > >Would really appreciate any help at all! > >Thank you, >Claire > > > > > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From bob-meyer <@t> northwestern.edu Tue May 16 10:34:51 2006 From: bob-meyer <@t> northwestern.edu (bob-meyer@northwestern.edu) Date: Tue May 16 10:34:56 2006 Subject: [Histonet] CD117 Message-ID: <20060516153451.3664035C66@casbah.it.northwestern.edu> They did have a mouse monoclonal antibody, but it was not recommended for formalin fixed paraffin embedded tissues. It was also a research use only antibody. The rabbit polyclonal is the preferred antibody for tissue, and it is for in vitro diagnostic use. Bob Meyer, HTL(ASCP),QIHC Sr. Research Technologist Northwestern University Pathology Core Facility 312-908-5546 ==============Original message text=============== On Mon, 15 May 2006 9:20:26 pm +0000 "Richard Cartun" wrote: Has Dako ever sold a mouse monoclonal or a rabbit monoclonal to CD117 (c-Kit)? Their current catalog only lists a rabbit polyclonal. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ===========End of original message text=========== From gcallis <@t> montana.edu Tue May 16 10:34:53 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue May 16 10:35:03 2006 Subject: flattening sections Re: [Histonet] Skin Artifact Identification In-Reply-To: <20060516124515.17700.qmail@web61211.mail.yahoo.com> References: <20060516124515.17700.qmail@web61211.mail.yahoo.com> Message-ID: <6.0.0.22.1.20060516093216.01b81f50@gemini.msu.montana.edu> Rene is correct on this. Also if you flatten your sections on a hot plate immediately after picking up the section onto the slides, this action will cause the same bubbling artifact. At 06:45 AM 5/16/2006, you wrote: >Santoianni & Hammami published an article in Jorunal >Histotechnol.[13(2):135-136(1990)] where they attribute a nuclear >"bubbling" artifact to oven heat-affixing the sections above 60?C before >they were completely free from water underneath. > I have been able to corroborate their finding. If this makes sense to > you try to be absolutely sure that there is no water left underneath your > sections before heating them in the oven. > Hope this will help you! > Ren? J. > >JGREWE@OhioHealth.com wrote: > Does anyone know what may be causing this skin artifact? What we have in >some of our skin cases is vacuoles within the nuclei of squamous cells of >epidermis on permanent sections. We don't see it all of the time. 10% >NBF fixation. Thanks >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Yahoo! Messenger with Voice. PC-to-Phone calls for ridiculously low rates. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From RCHIOVETTI <@t> aol.com Tue May 16 10:35:55 2006 From: RCHIOVETTI <@t> aol.com (RCHIOVETTI@aol.com) Date: Tue May 16 10:36:22 2006 Subject: [Histonet] looking for ventana email Message-ID: <2fe.54fc69a.319b4b5b@aol.com> In a message dated 5/15/2006 7:36:14 PM US Mountain Standard Time, lilbullrider00@yahoo.com writes: > am looking for Mike Reichenbach, or Debra Flynns email address i have a > couple of immuno questions for them... if any one could help that would be > great... thanks > > I can't be of specific help on this, but here's something to try: most of the folks at Ventana have email addresses which are their first initial followed by their last name. To try and make contact, you might want to send a message to Debra Flynn as: Hope this helps! Cheers, Bob Robert (Bob) Chiovetti, Ph.D. Owner The Microscope Works Arizona's Microscopy Resource 132 North Elster Drive Tucson, AZ 85710-3212 USA Tel./Fax 520-546-4986 Manufacturers' Representative Member, Arizona Small Business Association - ASBA From gcallis <@t> montana.edu Tue May 16 11:33:40 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue May 16 11:33:50 2006 Subject: [Histonet] Correction to Re: Autofluorescence/Imunofluorescence protocols rat kidney In-Reply-To: <6.0.0.22.1.20060516092457.01b556f8@gemini.msu.montana.edu> References: <4469b9af.7c.7cd1.1813511228@student.ucc.ie> <6.0.0.22.1.20060516092457.01b556f8@gemini.msu.montana.edu> Message-ID: <6.0.0.22.1.20060516103137.01b29078@gemini.msu.montana.edu> Please excuse the "DITC" mispelling, I meant FITC. I blame it on stumbling fingers on keyboard! Just a "DITC" day Gayle Callis At 09:31 AM 5/16/2006, you wrote: >You will get rid of autofluorescence or most of it by not fixing in an >aldehyde, this has been discussed recently and many times on Histonet, so >be sure to search archives for some explanations. DITC and your >autofluorescence are pretty much the same color. If you do snap frozen >fresh tissue, and another kind of fixation (acetone, acetone/alcohol) you >will have less problems here. OR you can try a different fluorophore, >i.e. a rhodamine RRX from Jackson OR a secondary conjugated to ALexa >555. Red color should come through with autofluorescence as the >counterstain, so to speak. > >It is not so important to see the perfect morphology enjoyed by PFA >fixation, but your IFA staining may improve greatly. You can use a >mounting media with DAPI also, this brings in the nuclei as a blue color - >for contrast and general morphology identification/location of antigen in >tissue. > >Molecular Probes also has secondaries conjugated to Alexa fluorophores, >very bright, excellent. > >I have a review of autofluorescence I am attaching to you privately, it is >very informative on fixation, and this problem, etc. > > > >At 05:38 AM 5/16/2006, you wrote: >>Hi Histonet, >> >>A very basic question but I am a complete novice in the >>world of immunohistochemistry / immunofluorescence and am >>trying to teach myself the rudiments! >> >>At present, I am attempting to visualise NHE3 in rat kidney >>using immunofluorescence but am encountering a lot of >>autofluorescence. I just wanted to enquire about possible >>reasons for / methods of dealing with this. >> >>My method is as follows: >> >>Once the kidneys have been excised I fix them in 4% >>paraformaldehyde overnight (4 degrees celsius) and then >>incubate them in 30% sucrose until they sink. Kidneys are >>then frozen in isopentane in liquid nitrogen and >>cryosectioned. >> >>Sections rinsed in distilled water >>3 X 5 min washes in PBS >>Incubation in blocking solution (3% normal goat serum, 0.25% >>Triton X. 96.75% PBS) for 1 hr @ room temp >>Slides then incubated with primary antibody (1:500 chemicon >>anti nhe3) in a humidified chamber @ 4 degrees overnight >> >>Slides rinsed in distilled water 3 X 5 min washes in PBS >>Incubation with 1:500 FITC conjugated secondary antibody for >>1 hr at room temp >>slides rinsed and coverslips applied with vectashield, >>sealed and visualised >> >>Apologies for all the detail, just generally unsure of my >>method... and whether there are more apt methodologies for >>this? Getting a lot of autofluorescence and finding it quite >>difficult to visualise the NHE3 at all! >> >>Would really appreciate any help at all! >> >>Thank you, >>Claire >> >> >> >> >> >> >> >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >Gayle Callis >MT,HT,HTL(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University - Bozeman >PO Box 173610 >Bozeman MT 59717-3610 >406 994-6367 >406 994-4303 (FAX) > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgirlnow <@t> msn.com Tue May 16 11:39:26 2006 From: godsgirlnow <@t> msn.com (Roxanne Soto) Date: Tue May 16 11:39:35 2006 Subject: [Histonet] 34BE12 Message-ID: Gotta question for you ... how many of you out there are running a positive and a negative control for your 34be12 on prostates? How many are not? How do you handle this in your procedure under your QC? I have been asked to do both. Some want a control and some don't I am trying to get a concensus and be uniform. The argument for no control.....it stains for normal tissue The arguement for a control....it still stains...... Any suggestions? Roxanne From Rcartun <@t> harthosp.org Tue May 16 11:48:38 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue May 16 11:49:08 2006 Subject: [Histonet] IHC Staining for Clostridium haemolyticum In-Reply-To: <001601c67879$1c9dd700$6401a8c0@VALUED20606295> References: <001601c67879$1c9dd700$6401a8c0@VALUED20606295> Message-ID: <4469CA2602000009000AEB35@hcnwgwds01.hh.chs> Dear Marilyn: My laboratory performs IHC staining for Clostridium sp. in formalin-fixed tissue using a polyclonal antibody from ViroStat in Portland, ME. I am not aware of a monoclonal antibody to C. haemolyticum. However, I would contact Douglas McAllister at ViroStat; he is very knowledgeable in this area. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Marilyn Johnson" 05/15/06 7:41 PM >>> Hi Histonetters, Sure hope someone out there can help me with this one! We are developing a project with our Parasitology Lab to demonstrate this bacterium, hopefully using a monoclonal primary antisera. Please include the source of reagents and staining procedure. Any replies would be greatly appreciated. Thank you in advance. Marilyn Johnson Alberta Agriculture Edmonton, Alberta, Canada. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Tue May 16 12:07:41 2006 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue May 16 12:08:01 2006 Subject: [Histonet] 34BE12 In-Reply-To: Message-ID: What if there is no "normal" tissue? "Roxanne Soto" Sent by: histonet-bounces@lists.utsouthwestern.edu 05/16/2006 09:39 AM To HISTONET@PATHOLOGY.SWMED.EDU cc Subject [Histonet] 34BE12 Gotta question for you ... how many of you out there are running a positive and a negative control for your 34be12 on prostates? How many are not? How do you handle this in your procedure under your QC? I have been asked to do both. Some want a control and some don't I am trying to get a concensus and be uniform. The argument for no control.....it stains for normal tissue The arguement for a control....it still stains...... Any suggestions? Roxanne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ploykasek <@t> phenopath.com Tue May 16 12:27:52 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Tue May 16 12:28:04 2006 Subject: [Histonet] 34BE12 In-Reply-To: Message-ID: Roxanne, Hopefully I've understood your question & I'll try my best to answer coherently! We do run a positive control for 34be12 - we use a normal prostate. Normal prostate is not too difficult to obtain. We are usually running 34be12 on several different cases covering different types of tissue. A separate positive control slide enables us to grade the staining whether or not there is any normal tissue staining on the patient material. On all of our positive controls we evaluate both the positive staining & the negative staining - both must be appropriate for the QC to be considered acceptable (this is stated in our SOP). We run a negative control for the patient material (if there are enough slides) to evaluate for any non-specific staining. I hope this answers your question. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Gotta question for you ... > how many of you out there are running a positive and a negative > control for your 34be12 on prostates? How many are not? How do you > handle this in your procedure under your QC? I have been asked to do > both. Some want a control and some don't I am trying to get a > concensus and be uniform. > The argument for no control.....it stains for normal tissue > The arguement for a control....it still stains...... > > Any suggestions? > Roxanne > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From jcline <@t> wchsys.org Tue May 16 12:30:50 2006 From: jcline <@t> wchsys.org (Joyce Cline) Date: Tue May 16 12:31:03 2006 Subject: [Histonet] 34BE12 In-Reply-To: Message-ID: <000b01c6790e$7a7bbaf0$1d2a14ac@wchsys.org> We use positive and negative controls. All our paths are in agreement, especially the path that is the prostate specialist. ____________________________________________________________________ Gotta question for you ... how many of you out there are running a positive and a negative control for your 34be12 on prostates? How many are not? How do you handle this in your procedure under your QC? I have been asked to do both. Some want a control and some don't I am trying to get a concensus and be uniform. The argument for no control.....it stains for normal tissue The arguement for a control....it still stains...... Any suggestions? Roxanne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From GDawson <@t> dynacaremilwaukee.com Tue May 16 12:41:42 2006 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Tue May 16 12:41:46 2006 Subject: [Histonet] 34BE12 Message-ID: Roxanne, I run a negative control and just the prostate slide itself for the 34be12 stain since prostate is, in fact, the appropriate control. When you run a piece of prostate for this antibody, it seems redundant to run another piece of prostate as a control. My Pathologists let me know if there is a problem with comments on the QC sheet, as is the case with all of my IHC cases. My Humble Procedure, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Roxanne Soto Sent: Tuesday, May 16, 2006 10:39 AM To: HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] 34BE12 Gotta question for you ... how many of you out there are running a positive and a negative control for your 34be12 on prostates? How many are not? How do you handle this in your procedure under your QC? I have been asked to do both. Some want a control and some don't I am trying to get a concensus and be uniform. The argument for no control.....it stains for normal tissue The arguement for a control....it still stains...... Any suggestions? Roxanne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Tue May 16 12:43:13 2006 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Tue May 16 12:43:15 2006 Subject: [Histonet] 34BE12 Message-ID: I have yet to run a 34BE12 stain on either a prostate bx or a prostate big that was completely negative for the stain. Glen Dawson -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jennifer MacDonald Sent: Tuesday, May 16, 2006 11:08 AM To: Roxanne Soto Cc: histonet-bounces@lists.utsouthwestern.edu; HISTONET@PATHOLOGY.SWMED.EDU Subject: Re: [Histonet] 34BE12 What if there is no "normal" tissue? "Roxanne Soto" Sent by: histonet-bounces@lists.utsouthwestern.edu 05/16/2006 09:39 AM To HISTONET@PATHOLOGY.SWMED.EDU cc Subject [Histonet] 34BE12 Gotta question for you ... how many of you out there are running a positive and a negative control for your 34be12 on prostates? How many are not? How do you handle this in your procedure under your QC? I have been asked to do both. Some want a control and some don't I am trying to get a concensus and be uniform. The argument for no control.....it stains for normal tissue The arguement for a control....it still stains...... Any suggestions? Roxanne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgirlnow <@t> msn.com Tue May 16 12:57:03 2006 From: godsgirlnow <@t> msn.com (Roxanne Soto) Date: Tue May 16 12:57:13 2006 Subject: [Histonet] 34BE12 In-Reply-To: Message-ID: Thank you Glen, This is what I have been doing...and I too have found that there is always some staining, there is always some small portion of normal tissue. It is highly unlikely that the entire core is completlely malignant. The only diffence is that we do not do a negative control......I know what CAP says. But we are not CAP. The doctors feel that since it is staining for normal tissue, a negative control is not necessary. Even on our other antibodies, we do not do a negative control per block--we only do a negative per patient......if out patient has 12 blocks and each has an antibody ordered, we still only do 1 negative. Any suggestions? Roxanne ______________________________________________________________ From: "Dawson, Glen" CC: histonet-bounces@lists.utsouthwestern.edu, HISTONET@PATHOLOGY.SWMED.EDU Subject: RE: [Histonet] 34BE12 Date: Tue, 16 May 2006 12:43:13 -0500 >I have yet to run a 34BE12 stain on either a prostate bx or a prostate big that was completely negative for the stain. > >Glen Dawson > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jennifer >MacDonald >Sent: Tuesday, May 16, 2006 11:08 AM >To: Roxanne Soto >Cc: histonet-bounces@lists.utsouthwestern.edu; >HISTONET@PATHOLOGY.SWMED.EDU >Subject: Re: [Histonet] 34BE12 > > >What if there is no "normal" tissue? > > > > > > > > >"Roxanne Soto" >Sent by: histonet-bounces@lists.utsouthwestern.edu >05/16/2006 09:39 AM > >To >HISTONET@PATHOLOGY.SWMED.EDU >cc > >Subject >[Histonet] 34BE12 > > > > > > > > Gotta question for you ... > how many of you out there are running a positive and a negative > control for your 34be12 on prostates? How many are not? How do you > handle this in your procedure under your QC? I have been asked to do > both. Some want a control and some don't I am trying to get a > concensus and be uniform. > The argument for no control.....it stains for normal tissue > The arguement for a control....it still stains...... > > Any suggestions? > Roxanne >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RohrT <@t> nyackhospital.org Tue May 16 13:55:53 2006 From: RohrT <@t> nyackhospital.org (Theresa Rohr) Date: Tue May 16 13:58:58 2006 Subject: [Histonet] CD10 Message-ID: Hi, I am just starting to work up the CD 10 Antibody (CALLA) Clone 56C6 from Novocastra. I am testing at a range of 1:50, 1:100, 1:200 and 1:300 using EDTA antigen retrieval in a steamer for 20 minutes with 20 minutes cooling and 60 minutes in primary antibody on the Dako Autostainer with the Envision+ protocol. Did any of you have any success with this antibody on renal cell carcinomas? My first efforts show only focal staining of the tumor in all the dilutions but 4+ internal control staining. My pathologists seem to expect more intense staining of the tumor cells. Any suggestions would be very well received. Thanks so much for listening. Theresa Rohr, Nyack Hospital NY From RohrT <@t> nyackhospital.org Tue May 16 13:57:45 2006 From: RohrT <@t> nyackhospital.org (Theresa Rohr) Date: Tue May 16 14:00:20 2006 Subject: [Histonet] CD10 Message-ID: Hi, I am just starting to work up the CD 10 Antibody (CALLA) Clone 56C6 from Novocastra. I am testing at a range of 1:50, 1:100, 1:200 and 1:300 using EDTA antigen retrieval in a steamer for 20 minutes with 20 minutes cooling and 60 minutes in primary antibody on the Dako Autostainer with the Envision+ protocol. Did any of you have any success with this antibody on renal cell carcinomas? My first efforts show only focal staining of the tumor in all the dilutions but 4+ internal control staining. My pathologists seem to expect more intense staining of the tumor cells. Any suggestions would be very well received. Thanks so much for listening. Theresa Rohr, Nyack Hospital NY Theresa Rohr, BA, HT(ASCP) Section Head, Histology Nyack Hospital 160 North Midland Avenue Nyack, New York 10960 phone 845-348-2276 fax 845-348-8430 Nyack Hospital is required by Law to protect the privacy of health information under HIPAA Rules and Regulations Part 11, Article 45 CFR, Parts 160-164. This email, including attachments, is intended for the exclusive use of the person or the entity to which it is addressed. It may contain confidential or legally protected information. The authorized recipient is prohibited from disclosing this information to any other party and is required to destroy this information after its stated need has been fulfilled. If the recipient or reader of this email is not the intended recipient or her/his authorized agent, the reader is hereby notified that any dissemination, distribution or copying of this email is prohibited. If you believe that you have received this email in error, please advise the sender immediately by reply email and then delete this email immediately. From spicin82 <@t> hotmail.com Tue May 16 14:02:47 2006 From: spicin82 <@t> hotmail.com (Lexus Ray) Date: Tue May 16 14:02:54 2006 Subject: [Histonet] blocking solution Message-ID: Hi, I'm a novice to immunolabeling. What are the advantages /disadvantages of using BSA in a blocking solution? Supposedly I used only normal sera in my blocking solution, would this result in high background resulting from primary antibody? L _________________________________________________________________ Be the one of the first to try the NEW Windows Live Mail. http://ideas.live.com/programPage.aspx?versionId=5d21c51a-b161-4314-9b0e-4911fb2b2e6d From Amanda.Garcia <@t> TriadHospitals.com Tue May 16 14:47:47 2006 From: Amanda.Garcia <@t> TriadHospitals.com (Garcia, Amanda) Date: Tue May 16 14:47:52 2006 Subject: [Histonet] (no subject) Message-ID: <8B63039C9DF4554C8FDBF31235F0E1480162CBED@CPRTEVS02.triadhospitals.net> Can anyone help me out with a question I was asked about JCAHO requirements regarding the statistical data being gathered of normal gallbladders, appendices and uteri by our pathology department? We have not included this in our quarterly surg dept stats in the past but a question was raised if we should be including this. Any info would be greatly appreciated. Thanks in advance > Amanda (Amy) Garcia > Histology/Pathology > College Station Medical Center > (979) 680-5372 office > (979) 696-5446 fax > *Mailto:amanda.garcia@triadhospitals.com > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the e-mail or any > of its attachments, please be advised that you have received this > e-mail in error and that any use, dissemination, distribution, > forwarding, printing, or copying of this e-mail or any attached files > is strictly prohibited. If you have received this e-mail in error, > please immediately purge it and all attachments and notify the sender > by reply e-mail or contact the sender at the number listed. > > > From gcallis <@t> montana.edu Tue May 16 15:57:31 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue May 16 15:57:46 2006 Subject: [Histonet] Ordering from DAKO or What I learned from my DAKO sales representative! Message-ID: <6.0.0.22.1.20060516141615.01b376b0@gemini.msu.montana.edu> Dear DAKO users. I had a long talk with my DAKO sales rep today, bless his heart!!! He explained the problems DAKO was having with the new computer system, which is being resolved asap. His sage advice for successful ordering was - have your purchasing agent or who ever places the order do the following: Avoid #1, go to #2, #3, or #4 in order to not be put on hold via slowphone forever and ever, amen! (Sorry Randy Travis!) 1. Don't phone in an order to avoid the long wait 2. FAX the order OR 3. email the order to Customer Service 4. Have your sale representative place the order for you. If you don't want to phone, FAX or email, the sales rep can do it for you to speed up the process. Back orders are lessening, and it is a long story as to why this all happened. The new computer system was not helping here either. Hope this helps Good luck on ordering - I'm doing it through my sales rep. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From nakagawa <@t> umn.edu Tue May 16 16:43:05 2006 From: nakagawa <@t> umn.edu (yasushi nakagawa) Date: Tue May 16 16:43:14 2006 Subject: [Histonet] In situ background on fresh frozen sections Message-ID: <219D59D5-7723-4178-898A-49A8E090FC8D@umn.edu> Hi, We are doing in situ using fresh frozen adult brain sections. We find some sense probes have strong labeling in areas with high cell density, such as dentate gyrus, cerebellum and rostral migratory stream. I wonder if any of you know specific solutions to get rid of this problem. Does RNase treatment help? Is it compatible with the following protocol? We use similar protocols for embryonic and early postnatal brain sections (except that we fix them before freezing), and work great. Our protocol: Freeze fresh brain in OCT on petri dish on liquid nitrogen Cut 20um cryosections Dry for a few days Fix 10 min in 4% PFA Wash in PBS Acetylation Wash in Triton X-100/PBS Wash in PBS Prehybridize for 1-2h (based on Denhart, salmon sperm DNA, yeast RNA, 50% formamide, SSC) Hybridize with ~100ng/ml DIG-labeled probes at 72 degree overnight (~14hr) Wash in 0.2xSSC for 1h at 72 degree Change to B1 solution Block for an hour (10% lamb serum in B1) Antibody incubation with anti-DIG (Roche, 1:5000) in the cold room overnight Wash in B1 Change buffer to B3 Do color reaction in NBT/BCIP with levamisole (B1 and B3 are Tris-based buffers used in Roche protocols) Thank you for the information. Yasushi From sbryant <@t> labpath.com Tue May 16 17:49:50 2006 From: sbryant <@t> labpath.com (Susan Bryant) Date: Tue May 16 17:55:46 2006 Subject: [Histonet] goat primary Ab Message-ID: <005201c6793b$2baedfb0$8d01a8c0@labpath.com> Hello to all I am sending this for a friend in research....how many folks out in Histoland are doing IHC with goat primaries on human, mouse and rat tissues? What detection is everyone using, and are you getting consistent results? You may send your responses to me and I will print them for her. Thanks for you time. Susan Bryant Knoxville Dermatopath From cfavara <@t> niaid.nih.gov Tue May 16 18:07:57 2006 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID) [E]) Date: Tue May 16 18:08:01 2006 Subject: [Histonet] goat primary Ab In-Reply-To: <005201c6793b$2baedfb0$8d01a8c0@labpath.com> Message-ID: I use a number of goat Abs on mouse using the Ventana Nexus - their detection modified avidin biotin system. c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives -----Original Message----- From: Susan Bryant [mailto:sbryant@labpath.com] Sent: Tuesday, May 16, 2006 3:50 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] goat primary Ab Hello to all I am sending this for a friend in research....how many folks out in Histoland are doing IHC with goat primaries on human, mouse and rat tissues? What detection is everyone using, and are you getting consistent results? You may send your responses to me and I will print them for her. Thanks for you time. Susan Bryant Knoxville Dermatopath _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cfavara <@t> niaid.nih.gov Tue May 16 18:16:30 2006 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID) [E]) Date: Tue May 16 18:16:38 2006 Subject: [Histonet] blocking solution In-Reply-To: Message-ID: In my hands BSA often causes more problems than it solves. One needs to be careful of the grade used, it is very 'sticky' and I have had more non-specific background staining using BSA than with just about anything else. We previously have studied mostly rodent brain and eye which I have found to have very little background problems. Also if you are using and goat primary you can get some cross-reactivity with BSA and some anti-goat IgG's depending on purity, specificity etc. So to get to the heart of the matter I only block for endogenous peroxidase unless I have a problem and then I will block with normal serum of the host species of the secondary. I am becoming a minimalist! c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives -----Original Message----- From: Lexus Ray [mailto:spicin82@hotmail.com] Sent: Tuesday, May 16, 2006 12:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] blocking solution Hi, I'm a novice to immunolabeling. What are the advantages /disadvantages of using BSA in a blocking solution? Supposedly I used only normal sera in my blocking solution, would this result in high background resulting from primary antibody? L _________________________________________________________________ Be the one of the first to try the NEW Windows Live Mail. http://ideas.live.com/programPage.aspx?versionId=5d21c51a-b161-4314-9b0e -4911fb2b2e6d _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From katri <@t> cogeco.ca Tue May 16 18:40:11 2006 From: katri <@t> cogeco.ca (Katri Tuomala) Date: Tue May 16 18:40:11 2006 Subject: [Histonet] goat primary Ab References: <005201c6793b$2baedfb0$8d01a8c0@labpath.com> Message-ID: <011a01c67942$12b78fa0$6a9a9618@Katri> I'm using Rage (Serotec) antibody raised in goat on human brain tissue. My detection is Zymed's biotinylated rabbit-anti goat and streptavidin. Works well at 1:3000 dilution after protease digestion. Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "Susan Bryant" To: Sent: Tuesday, May 16, 2006 6:49 PM Subject: [Histonet] goat primary Ab Hello to all I am sending this for a friend in research....how many folks out in Histoland are doing IHC with goat primaries on human, mouse and rat tissues? What detection is everyone using, and are you getting consistent results? You may send your responses to me and I will print them for her. Thanks for you time. Susan Bryant Knoxville Dermatopath _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Tue May 16 18:55:20 2006 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Tue May 16 18:54:32 2006 Subject: [Histonet] goat primary Ab In-Reply-To: <005201c6793b$2baedfb0$8d01a8c0@labpath.com> References: <005201c6793b$2baedfb0$8d01a8c0@labpath.com> Message-ID: Hi Susan, I use goat primaries on mouse and human tissue routinely with consistent and acceptable results. I use Jackson's anti-goat secondaries for detection. I choose the Donkey anti-Goat IgG "ML" secondaries to reduce species cross reactivity. Works well. Contact me directly if you need more information. -- Andrea >Hello to all > > I am sending this for a friend in research....how many folks out in >Histoland are doing IHC with goat primaries on human, mouse and rat >tissues? What detection is everyone using, and are you getting >consistent results? > > You may send your responses to me and I will print them for her. >Thanks for you time. > >Susan Bryant >Knoxville Dermatopath >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- From abilger <@t> ptd.net Tue May 16 19:03:06 2006 From: abilger <@t> ptd.net (Andrea C. Bilger) Date: Tue May 16 19:04:34 2006 Subject: [Histonet] Tech mislabeling slides Message-ID: <053501c67945$4930d840$0300a8c0@andrea> In our lab we have 2 identifiers on our cassettes and preprinted slide labels, the patient name and the surgical specimen number. We use a cassette labeler integrated with our lab computer system to print the cassettes. Slide labels are generated automatically using the lab system also. This reduced errors. Our next problem was placing the correct slide label on the slide. We now have the techs look at every block before placing in the microtome chuck and look at the patient name as well as the number. Then they look at the slide label and check to see that the same name and number is on that before sticking it on the slide. Almost anyone can transpose a number but the name is easily recognized. I think I look at names more than numbers now. It has really helped us reduce our labeling errors. Andrea Bilger From jfern <@t> unimelb.edu.au Tue May 16 21:11:45 2006 From: jfern <@t> unimelb.edu.au (John Fernandez) Date: Tue May 16 21:12:00 2006 Subject: [Histonet] Re: Golgi stain question/Rapid Golgi Stain (FDNeurotech) In-Reply-To: <4468A57E.FA5006AB@uwo.ca> References: <1706.128.250.186.193.1147241410.squirrel@webmail.unimelb.edu.au> <446354D3.D15CBAB3@uwo.ca> <2338.128.250.186.193.1147658214.squirrel@webmail.unimelb.edu.au> <4468A57E.FA5006AB@uwo.ca> Message-ID: <3169.128.250.186.193.1147831905.squirrel@webmail.unimelb.edu.au> John thanks again, I should have been more clear: the method advocated by the FD Neurotech kit does involve freezing the tissue, cutting (100-200um thick) sections on a cryostat and slide mounting on gelatin-coated slides (this is all carried out 2 weeks post incubation with the Potassium Dichromate + Mercuric Chloride/Potassium Chromate solutions. After the sections are slide mounted, they are left to dry in the dark at room temeperature for 1-2 weeks after which they undergo the staining procedure with solns C & D. The kit then advises dehydration in an ethanol gradient followed by xylene and coverslipping with a resinous mounting medium (we use DPX). Can you suggest any intermediary step between immersion in solutions C+D and dehydration/mounting that might halt the stain degradation that occurs post cover-slipping? Thanks in advance, John > Your kit's literature declares itself (partly). It > is neither rapid nor Golgi, and certainly not the > classical rapid Golgi method! It is evidently a > "Golgi-Cox" method, perhaps based on Sholl's > modification or one of Kolb's much more recently > published variants. These are used for looking at > dendrites (branching patterns, spines). The > secret items C & D in the kit will be for the > alkaline reduction step that deposits the black > stuff. In published methods this is usually a 1:20 > dilution of ammonium hydroxide (.880 ammonia) in > water. A kit could generate an equivalent solution > by mixing two components (such as any ammonium > salt and any stronger base - KOH, NaOH, Na2CO3) in > a prescribed volume of water. The calculation is > an elementary one. > > To answer your question about fixation: > > The mercuric chloride/dichromate/chromate mixture > serves as the fixative in methods of this type. > > You say that the kit advises moving the blocks > into sucrose; presumably this is for cutting > frozen sections. But you also talk about > dehydrating in alcohol to "stop the reaction". > Reaction-stopping with alcohol isn't part of any > Golgi or Cox method. With any histological > procedure involving dichromate as a fixative or > other agent, thorough washing in water (hours; > slowly running, or plenty of changes) is necessary > to remove unbound dichromate before going into > alcohol, with which there is a chemical reaction > that leads to precipitation of Cr2O3. > > John Kiernan > Anatomy, UWO > London, Canada. > _____________________________________- > John Fernandez wrote: >> John, thanks for your reply; >> the kit contents are of the Cox'x variety. >> That is, solution A of the "FD Rapid GolgiStain Kit" contains Potassium Dichromate + Mercuric Chloride. >> Solution A is added to solution B (Potassium Chromate) and the brain tissue is left to be impregnated with this mixture for two weeks after which it is transferred to a sucrose solution for 4 days before being cut >> and slide mounted and put through further staining with solutions D & E (contents of which the company has not disclosed). >> With the Cox's method that you know of, is there a fixation step (other than dehydrating in ethanol gradient and xylene) that is undertaken to stop the reaction? >> As I have mentioned we do get excellent initial staining but the problem >> is that it continues on even after coverslipping. >> Thanks, >> John F >> > The first thing you need to find out is whst's in >> > the kit. >> > >> > Is the method the one traditionally known as the >> > rapid Golgi method? This involves fixation in an >> > osmium tetroxide-potassium dichromate mixture, for >> > 2 to 7 days (the time affects the outcome, but >> > unpredictably) followed by immersion in aqueous >> > silver nitrate for a few days, and preparing thick >> > celloidin or paraffin sections. There are plenty >> > of later variants, having in common a silver >> > chromate end product, but these should not be >> > called "the rapid Golgi method". The sections are >> > mounted in thick Canada balsam (the real stuff) >> > without coverslips. If a coverslip is applied, the >> > preparations fade with time. There are various >> > chemical tricks to prevent such fading. >> > >> > The other major member of this group of methods is >> > Cox's, with mercuric chloride and potassium >> > dichromate, but no silver. The dark deposit in >> > this case is thought to be a mixture of mercurous >> > oxide and colloidal mercury. These methods are >> > often called Golgi-Cox, even though Golgi didn't >> > invent or use them. Ideally Golgi-Cox preparations >> > should also be mounted without coverslips, but >> > they will keep for a few years in DPX with >> > coverslips. >> > >> > John Kiernan >> > Anatomy, UWO >> > London, Canada >> > ____________________ >> > John Fernandez wrote: >> >> >> >> Hi all, >> >> >> >> our lab has recently purchased and used the Rapid Golgi Stain Kit >> from >> >> FD >> >> Neurotech, resulting in very impressive staining down to dendritic >> spine >> >> level, however as Julie Heinrich found, within 24 hours this staining >> >> becomes very granular with loss of distinct morphology that was otherwise >> >> seen previously. It progressively worsens over time. >> >> >> >> Has anyone successfully used the FD Neurotech Rapid Golgi kit? Or >> does >> >> anyone know of a mounting medium that can be used to adequately stop >> the >> >> reaction that is taking place? Any ideas on what could be causing >> this >> >> progressive deterioration of staining would be very much appreciated. >> >> >> >> Thanks in advance, >> >> >> >> John >> >> >> >> John Fernandez >> >> Research Assistant >> >> Department of Medicine >> >> University of Melbourne >> >> Austin & Repatriation Medical Centre >> >> Heidelberg, VIC 3084 >> >> Ph: (03) 9496 3257 >> >> >> >> Re: Golgi stain question >> >> From: "J. A. Kiernan" >> >> >> >> -------------------------------------------------------------------------------- >> >> >> >> On Fri, 30 Mar 2001, Julie Heinrich wrote: >> >> >> >> > I am terribly sorry to bother you- I have been looking through the histonet web page and have found several helpful replies from you about the Gibb & Kolb Golgi stain method. I haven't managed to figure out how to properly post a question there - >> >> >> >> You can't. It's a collection of old (archived) communications. To ask or answer questions you have to subscribe to the >> >> listserver. This is done by sending an email to >> >> histonet@pathology.swmed.edu with the one word subscribe >> >> in the Subject line. Nothing else. You'll then get an automatic reply from the listserver telling you all about it. >> >> >> >> > I'm attempting to use the method on avian tissue. I have obtained some decent looking tissue so far (though the stain is a dark tan/brown, rather than the preferable black that I had expected) >> yet >> >> > after time the stain turns very 'grainy'. Within a matter of just >> 24 >> >> > hours, the dendrites/spines look like collections of dots rather >> than >> >> > complete structures, and it gets worse with time. >> >> >> >> > Do you have any idea why this might be happening? (I'm following their protocol to the best of my knowledge, and use fresh solutions >> >> > each time I run tissue) >> >> >> >> A graduate student here called Tim Ho did great numbers of Kolb Golgis on rat brains a few years ago. He went to Kolb's lab in Lethbridge, Alta for guidance. His initial problem was that the unstained spaces between the black cells were pale green and a bit granular. If I remember rightly, the washing after the Golgi-Cox solution needed to be more thorough. >> >> >> >> Your problem is different, and may relate to the mounting medium. Traditionally the sections were mounted in thick Canada balsam without coverslips. A modern synthetic medium + coverslip can be followed by fading, but I haven't heard of this happening in 24 hours. 6 months, yes. Are you cutting vibratome sections of adequate thickness? I think you must be doing something wrong at or after the sectioning step, but don't know what. Sorry I can't be more helpful. >> >> >> >> ---------------------------------------- >> >> John A. Kiernan >> >> Department of Anatomy & Cell Biology >> >> The University of Western Ontario >> >> London, Canada N6A 5C1 >> >> kiernan@uwo.ca >> >> http://publish.uwo.ca/~jkiernan >> >> >> >> -------------------------------------------------------------------------------- >> >> >> >> << Previous Message | Next Message >> >> >> -- >> >> >> >> _______________________________________________ >> >> Histonet mailing list >> >> Histonet@lists.utsouthwestern.edu >> >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > >> -- >> John Fernandez >> Research Assistant >> Department of Medicine >> University of Melbourne >> Austin & Repatriation Medical Centre >> Heidelberg, VIC 3084 >> Ph: (03) 9496 3257 > -- John Fernandez Research Assistant Department of Medicine University of Melbourne Austin & Repatriation Medical Centre Heidelberg, VIC 3084 Ph: (03) 9496 3257 From c.m.vanderloos <@t> amc.uva.nl Wed May 17 01:56:46 2006 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Wed May 17 01:56:56 2006 Subject: [Histonet] RE: immunofluorescence amplification... Message-ID: Dear Adam, The best way of of amplifying fluorescence signal is using a tyramide-based detection system. There are kits available from Perkin&Elmer and Molecular Probes/Invitrogen. First you detect your primary with a HRP-labeled secondary and then the HRP activity is employed for the amplification reaction ending up with a fluorochrome of your choice. Also fluorescence double staining is described performing the amplification reaction sequentially with a peroxidase blocking in between [Speel et al. 1997, JHC 45:1439-1446: Sensitive multicolor fluorescence in situ hybridization using catalized reporter deposition (CARD) amplification]. Good luck! Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Mon, 15 May 2006 16:44:28 -0700 (PDT) From: Adam Perry Subject: [Histonet] immunofluorescence amplification... To: histonet@pathology.swmed.edu What are people's impressions about different (i.e. the best) ways to amplify fluorescent signal from immunocytochemistry? I'm working with 30 um rodent brain sections. When I use a biotinylated secondary antibody with avidin-biotin-HRP detection with DAB or NiDAB I get good cellular staining with a primary antibody concentration of 1:1,000. I am now trying to perform double labeling and so have switched to fluorescence...and I don't seem to be getting any specific signal now. I have tried increasing the antibody concentration from 1:1,000 up to 1:100 and still don't see the faintest hint of staining (and the background just gets really bad). I've used the fluorescent antibody to detect other primaries (so the secondary is working in general and my mi! croscope From jatindra_prakash <@t> yahoo.com Wed May 17 02:31:14 2006 From: jatindra_prakash <@t> yahoo.com (Jatindra Prakash) Date: Wed May 17 02:31:18 2006 Subject: [Histonet] Incubation chamber Message-ID: <20060517073114.43973.qmail@web32914.mail.mud.yahoo.com> Does anyone know of a supplier for a low cost incubation chambers for hold up to 6 slides? I am tired of having slides dry out during overnight incubation in the make-shift chambers I have tried to construct. Thanks in advance, Jatindra Atlantic Veterinary College Canada --------------------------------- How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. From dennijc <@t> vetmed.auburn.edu Wed May 17 07:38:13 2006 From: dennijc <@t> vetmed.auburn.edu (John C. Dennis) Date: Wed May 17 07:38:27 2006 Subject: [Histonet] goat primary Ab In-Reply-To: <005201c6793b$2baedfb0$8d01a8c0@labpath.com> References: <005201c6793b$2baedfb0$8d01a8c0@labpath.com> Message-ID: Susan I use Vector's biotin conjugated rabbit antigoat and Molecular Probes Alexa 488 rabbit antigoat. They both work nicely for the anti-OMP that I use. John Carroll Dennis Anatomy, Physiology, and Pharmacology 109 Greene Hall Auburn University, AL 36849 On Tue, 16 May 2006, Susan Bryant wrote: > Hello to all > > I am sending this for a friend in research....how many folks out in Histoland are doing IHC with goat primaries on human, mouse and rat tissues? What detection is everyone using, and are you getting consistent results? > > You may send your responses to me and I will print them for her. Thanks for you time. > > Susan Bryant > Knoxville Dermatopath > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From kmerriam2003 <@t> yahoo.com Wed May 17 08:17:43 2006 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Wed May 17 08:17:50 2006 Subject: [Histonet] goat primary Ab In-Reply-To: <005201c6793b$2baedfb0$8d01a8c0@labpath.com> Message-ID: <20060517131743.41850.qmail@web50315.mail.yahoo.com> Susan, I have done a lot of work with goat primaries in mouse tissues. I normally use the Vector Elite ABC kit (Goat IgG) for detection; 30 minutes in anti-goat secondary, 30 minutes in ABC reagent (both at room temp). I am beta testing a goat polymer kit for Biocare this week, it should be available within the next few weeks or so (I am doing the run on Friday, so I can't comment on it yet). I am sure it will work well, I love their mouse and rabbit polymer kits! Kim Merriam, MA, HT(ASCP) Novartis Cambridge, MA Susan Bryant wrote: Hello to all I am sending this for a friend in research....how many folks out in Histoland are doing IHC with goat primaries on human, mouse and rat tissues? What detection is everyone using, and are you getting consistent results? You may send your responses to me and I will print them for her. Thanks for you time. Susan Bryant Knoxville Dermatopath _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. From rjbuesa <@t> yahoo.com Wed May 17 09:22:27 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 17 09:22:31 2006 Subject: [Histonet] Incubation chamber In-Reply-To: <20060517073114.43973.qmail@web32914.mail.mud.yahoo.com> Message-ID: <20060517142227.70931.qmail@web61224.mail.yahoo.com> You can safely incubate your slides by placing them in one plastic slide box designed for 100 slides. Just place enough really damped paper towels on the bottom, place the slides flat accross over the slides slots, close the box and they will be fine the next morning. Hope this will help you! Ren? J. Jatindra Prakash wrote: Does anyone know of a supplier for a low cost incubation chambers for hold up to 6 slides? I am tired of having slides dry out during overnight incubation in the make-shift chambers I have tried to construct. Thanks in advance, Jatindra Atlantic Veterinary College Canada --------------------------------- How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Sneak preview the all-new Yahoo.com. It's not radically different. Just radically better. From PMonfils <@t> Lifespan.org Wed May 17 09:23:11 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed May 17 09:23:19 2006 Subject: [Histonet] DDK-plast? Message-ID: <09C945920A6B654199F7A58A1D7D1FDE01717700@lsexch.lsmaster.lifespan.org> A paper I am reading calls for embedding in DDK-plast. I have never heard of this embedding medium. From the context it sounds like it must be similar to methyl methacrylate. Has anyone used this? What can you tell me about it? Especially how it compares to typical PMMA. Thanks. From gcallis <@t> montana.edu Wed May 17 10:25:50 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed May 17 10:26:00 2006 Subject: BSA quality RE: [Histonet] blocking solution In-Reply-To: References: Message-ID: <6.0.0.22.1.20060517085427.01b45148@gemini.msu.montana.edu> Dear L "the novice at immunolabeling" and asking about BSA Question: Did you have a problem with your staining after using a normal serum block? If so, let us know what happened - we can always help with specifics too. What Cynthia discussed about BSA is important. Jackson ImmunoResearch catalog/website discusses the caveats about using BSA with large animal species. If you need to use BSA, it must be of highest molecular biology quality or buy Jackson's immunoglobulin and protease free BSA. If you purchase BSA from Sigma, look for the same quality as Jacksons. If you look at Jackson data/specification sheets for their antibodies, the protein carrier is their BSA. We choose to use normal serum blocking exclusively, as Cynthia discussed, for our animal work, even other than mouse. BSA is reserved for special types of staining,i.e. lectin where serums containing carbohydrate or sugar residues can bind to lectins. For clinical i.e. human work, many of the blocks contain BSA and work fine, sold ready to use - these have not worked well in my hands for my murine IHC or IFA work. There are other serum-free blocks available some with casein to try. Some good reading can be found for free, DAKO Handbook of Immunochemical Staining, 3rd edition, you can download the pdf and print it off. There are also some good, inexpensive books to help you. Introduction to Immunocytochemistry, 3rd Edition Plak and van Noorden (ISBN#1-85996-208-4) Amazon.com Immunochemistry 2, a practical approch. Ed. Johnstone AP, and Turner MW (ISBN# 0 19 963609 5) Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 At 05:16 PM 5/16/2006, you wrote: >In my hands BSA often causes more problems than it solves. One needs to >be careful of the grade used, it is very 'sticky' and I have had more >non-specific background staining using BSA than with just about anything >else. We previously have studied mostly rodent brain and eye which I >have found to have very little background problems. Also if you are >using and goat primary you can get some cross-reactivity with BSA and >some anti-goat IgG's depending on purity, specificity etc. > >So to get to the heart of the matter I only block for endogenous >peroxidase unless I have a problem and then I will block with normal >serum of the host species of the secondary. I am becoming a minimalist! > >c > >Cynthia Favara >NIAID/NIH/RML/LPVD >903 South 4th Street >Hamilton, MT 59840 >406-363-9317 From anh2006 <@t> med.cornell.edu Wed May 17 10:32:05 2006 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Wed May 17 10:31:14 2006 Subject: [Histonet] blocking solution In-Reply-To: References: Message-ID: I use both BSA and normal serum from my secondary to block when I am staining both human and murine tissues with no problems. My BSA is molecular grade fraction, I am happy to share the catalog number should you need it. > >-----Original Message----- >From: Lexus Ray [mailto:spicin82@hotmail.com] >Sent: Tuesday, May 16, 2006 12:03 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] blocking solution > >Hi, >I'm a novice to immunolabeling. >What are the advantages /disadvantages of using BSA in a blocking >solution? >Supposedly I used only normal sera in my blocking solution, would this >result in high background resulting from primary antibody? > >L > -- From ploykasek <@t> phenopath.com Wed May 17 10:40:34 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Wed May 17 10:40:44 2006 Subject: [Histonet] M2 fixative Message-ID: Hi all. I was wondering if anyone has heard of or used a fixative called M2. I received a specimen in M2 fixative with no other info. A quick on-line search did not yield much information - only found out it is mercury based. As always, thanks for the help. Patti Loykasek PhenoPath ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From mtitford <@t> aol.com Wed May 17 10:43:46 2006 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Wed May 17 10:43:58 2006 Subject: [Histonet] "Normal" tissue statistics Message-ID: <8C847DFD1B57688-116C-11E9@FWM-D02.sysops.aol.com> Amanda Garcia at College Station Medical Center asks about statistics on "normal" tissue. Amanda, before you go off investing time in this project, you may want to check to see if your hospital has a quality assurance type committee that collects this information already. In our hospital it is (or was when I served on it) called the "Surgical Case Review Committee". The committee was a requirement of JCAHO (I think). It checked patients charts to make sure their operations were necessary. It had ongoing projects to survey different type procedures to make sure they were necessary too. ( We got to sit in plush chairs in the boardroom and they fed us lunch...!) Mike Titford USA Pathology Mobile AL USA From gcallis <@t> montana.edu Wed May 17 11:10:55 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed May 17 11:11:06 2006 Subject: [Histonet] DDK-plast? In-Reply-To: <09C945920A6B654199F7A58A1D7D1FDE01717700@lsexch.lsmaster.l ifespan.org> References: <09C945920A6B654199F7A58A1D7D1FDE01717700@lsexch.lsmaster.lifespan.org> Message-ID: <6.0.0.22.1.20060517100054.01b85a88@gemini.msu.montana.edu> Paul, It was a MMA kit sold by Delaware Diamond Knives for many years. They no longer sell this and haven't for many years. I recall there was a problem in getting the plasticizer (it was different from dibutyl phthlate) from Europe and it may have been because this plasticizer was no longer being produced. DDK discontinued setting up and selling the kits. One can buy a similar kit, Technovits 9100 (a MMA kit) for the same kind of application and results or you can make up your own inhouse mixtures for PMMA - the latter is what we used to do. The K-Plast kit was very good, it always seemed a bit softer for grinding, but the staining was very good. We preferred a harder PMMA but hardness can be adjusted several ways to fit one's needs, our preference. The Technovits 9100 should be comparable and very efficient in kit form. We processed undecalcified bone for infiltration with K-Plast in the exact same way as using a inhouse mixture or how people use Technovits 9100. I still have package inserts fro K-Plast in my bone files. t 08:23 AM 5/17/2006, you wrote: >A paper I am reading calls for embedding in DDK-plast. I have never heard of >this embedding medium. From the context it sounds like it must be similar to >methyl methacrylate. Has anyone used this? What can you tell me about it? >Especially how it compares to typical PMMA. Thanks. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From eric <@t> ategra.com Wed May 17 12:23:02 2006 From: eric <@t> ategra.com (Eric Dye) Date: Wed May 17 12:10:41 2006 Subject: [Histonet] Many Brand N E W Histology openings since my last email Message-ID: <20060517171032.KTGY20587.rrcs-fep-12.hrndva.rr.com@Pamsgx110> Just a quick update - Below I am listing many brand N E W Histology openings since my last email. If you are interested in any of the j ob s listed below or anywhere else - please call me at 800-466-9919 ext 223 or cell - 407-756-5507. Most Histo jobs are full-time dayshift (Except where indicated below) Here are some of my Newest Histology Jobs: ------------------------------------------ 01. Savannah, Georgia - HistoTech - Perm - must be A S C P (HT or HTL) - dayshift 02. Upstate Region, S. Carolina - Perm - 2 j ob s: HistoTech & HistoSupv - days 03. Boca Raton, Florida - temp - HistoTech 04. Florida, Naples/Ft Myers - temp - Bench Histotech 05. Pennsylvania, Pittsburgh - perm - Bench HistoTech - days 06. Washington, Spokane - perm - Bench HistoTech - days 07. Virginia/DC Beltway East - Fairfax Area - Perm, Histo Tech Update on Current Histology Jobs: --------------------------------- 01. Northern New Jersey (Passaic/Paramus Area) - Perm and Temp - 2 openings, Bench Histo Tech, 1st shift 02. Rhode Island, Providence - Perm, Bench Histo Tech, 2nd Shift 03. filled 04. Ohio, Columbus - Perm and Temp -2 openings, Bench Histo Tech, many shifts 05. Ohio, Dayton - Perm and Temp - 2 openings, Bench Histo Tech, many shifts 06. New York, Central Long Island - Perm, Supervisor Histo Tech 07. filled 08. Virginia/DC Beltway South - South of Metro DC , Perm, Lead Histo Tech 09. Florida, Pompano Beach - Perm, Supervisor Histo Tech 10. Florida, Pompano Beach - Perm, Bench Histo Tech 11. Mass, Boston - Part-time, Perm, Bench Histo Tech 12. West Virginia - Perm, Bench Histo Tech 13. filled 14. Georgia, Metro Atlanta area - Perm, Bench Histotech 15. New Hampshire -Mohs and Routine Bench Histotech,Perm and Temp 16. filled 17. Florida, Naples/Ft Myers - perm - Bench Histotech 18. Ohio -Central -Temp, Bench Histotech 19. filled 20. South Carolina -Charleston Area -Fulltime, Perm Thank you, Eric Dye-Sr Allied Healthcare Recruiter 800-466-9919 ext 223 or Cell - 407-756-5507 PS: Feel free to pass along this email and my phone number to anyone who you think might be interested. PSS: The clients are currently interviewing - and the job will close soon - so if you are interested, please call me today at 1-800-466-9919 ext 229 --------------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing 800 466 9919 ext 223 - voice 407 671 6075 - fax eric@ategra.com To Learn More About Ategra: http://www.ategra.com ---------------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. ---------------------------------------------------------------- From ttroyer <@t> petersonlab.com Wed May 17 12:36:22 2006 From: ttroyer <@t> petersonlab.com (Travis Troyer) Date: Wed May 17 12:36:26 2006 Subject: [Histonet] portable cryostat Message-ID: <000801c679d8$6ad86060$6601010a@Peterson.local> Our pathologists are in the market for a portable cryostat. Does anyone have a suggestion or pros and cons on a cryostat. Thank you, Travis From Lori.Karnes <@t> saskatoonhealthregion.ca Wed May 17 13:21:04 2006 From: Lori.Karnes <@t> saskatoonhealthregion.ca (Karnes, Lori SktnHR) Date: Wed May 17 13:21:33 2006 Subject: [Histonet] Continuous process Message-ID: <7A83E83CB5B81A4A83F6A8268392B0C2848557@stampy.sktnhr.ca> Hello: our lab has just been through a LEAN review and we are looking at continuous process of our Histology Specimens. I was wondering if anyone has changed their lab process to accommodate this? Our lab runs only 8 hours/day and not 24hrs. I can understand the benefits of this for a 24hr lab but not an 8 hour one. Any help would be appreciated. Thanks in advance Lori Karnes ART Tech III, Histology Laboratory Saskatoon Health Region Phone: (306) 655-8197 Email: lori.karnes@saskatoonhealthregion.ca From akbitting <@t> geisinger.edu Wed May 17 14:04:49 2006 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Wed May 17 14:05:23 2006 Subject: [Histonet] Sakura Xpress users Message-ID: I'd love to get opinions from hospital Histology labs using the Xpress processor. Our Director went off to "War College" and heard all about it and you all know what happens next............. What are the benefits and drawbacks to it? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From Jackie.O'Connor <@t> abbott.com Wed May 17 14:17:13 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed May 17 14:17:41 2006 Subject: [Histonet] Xgal In-Reply-To: <000801c679d8$6ad86060$6601010a@Peterson.local> Message-ID: I've been asked to cut frozen setions for XGal staining (to be performed by someone else). Any suggestions? These tissue samples were snap frozen (no OCT) in LN2. Any reason to NOT use OCT to orient them for sectioning? Thanks. Jackie From anh2006 <@t> med.cornell.edu Wed May 17 14:27:00 2006 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Wed May 17 14:26:16 2006 Subject: [Histonet] Xgal In-Reply-To: References: Message-ID: Frozen sections for XGal staining works beautifully. OCT is fine. Dry for a few hours then stain. >I've been asked to cut frozen setions for XGal staining (to be performed >by someone else). Any suggestions? These tissue samples were snap frozen >(no OCT) in LN2. Any reason to NOT use OCT to orient them for sectioning? >Thanks. > >Jackie >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- From pdc96 <@t> hotmail.com Wed May 17 15:35:09 2006 From: pdc96 <@t> hotmail.com (Paul Cremers) Date: Wed May 17 15:35:18 2006 Subject: [Histonet] employment Message-ID: I am looking for a job in the Boulder/Fort Collins area. My wife and I plan to move in late July. Please let me know if anyone is looking to fill a histotechnician position. Regards, Paul Cremers From c.m.vanderloos <@t> amc.uva.nl Thu May 18 01:45:33 2006 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Thu May 18 01:45:52 2006 Subject: [Histonet] RE: goat primary Ab Message-ID: <26483326311d.26311d264833@amc.uva.nl> Dear Susan, So far I have seen just 2-step and 3-step streptavidin-biotin methods coming by. We have tried here two biotin-free systems: 1) anti-goat polymer-HRP from Zymed/Invitrogen: it works nice with clean background. However, the performance of this polymer is much less compared with a 3-step streptavidin-biotin method (note: anti-rabbit and -mouse polymers like EnVision+ and PowerVision are nearly as sensitive as a 3-step streptavidin-biotin method). 2) 3-step approach with first rabbit anti-goat (Dako, 1/2500-5000, 30 min) followed by an anti-rabbit polymer-HRP. This is quite a sensitive method that worked very good for us for a number of goat primaries. Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Tue, 16 May 2006 18:49:50 -0400 From: "Susan Bryant" Subject: [Histonet] goat primary Ab To: Hello to all I am sending this for a friend in research....how many folks out in Histoland are doing IHC with goat primaries on human, mouse and rat tissues? What detection is everyone using, and are you getting consistent results? You may send your responses to me and I will print them for her. Thanks for you time. Susan Bryant Knoxville Dermatopath From kemlo.rogerson <@t> waht.swest.nhs.uk Thu May 18 01:55:17 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu May 18 01:55:01 2006 Subject: [Histonet] Continuous process Message-ID: This is an interesting question; I contemplated continuous processing when I was a Cell Path Manager, but for the 'average' Histo Lab what would be the benefits? I guess if you concatenated a few middle sized Labs (?3) you could move to 24/7 and have a Hub Lab just like Cytology and LBC. The wild cards (as ever) would be the Pathologists; bit like hamsters IMHO. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 The past is never dead, it is not even past. --William Faulkner This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From ree3 <@t> leicester.ac.uk Thu May 18 05:43:59 2006 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Thu May 18 05:44:08 2006 Subject: [Histonet] section thickness Message-ID: In your invaluable, but, no doubt differing opinions, what is the optimal section thickness for formalin fixed paraffin processed tissues for immunostaining, using DAB as chromogen?, there has been some discussion here whether it should be 4,5,6, or 7microns, many thanks. Richard Edwards MRC TOXICOLOGY UNIT LEICESTER..U.K....... From louise.renton <@t> gmail.com Thu May 18 05:53:30 2006 From: louise.renton <@t> gmail.com (louise renton) Date: Thu May 18 05:53:36 2006 Subject: [Histonet] 3D recontruction Message-ID: Hi, I would like to get in touch with someone doing this type of research, specifically to ask questions as to how many, what thickness and staining to use on sections many thanks -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin From GDawson <@t> dynacaremilwaukee.com Thu May 18 07:40:38 2006 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Thu May 18 07:40:48 2006 Subject: [Histonet] section thickness Message-ID: I do all of my immunostaining @ 3 microns. Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Edwards, R.E. Sent: Thursday, May 18, 2006 4:44 AM To: histonet@pathology.swmed.edu Subject: [Histonet] section thickness In your invaluable, but, no doubt differing opinions, what is the optimal section thickness for formalin fixed paraffin processed tissues for immunostaining, using DAB as chromogen?, there has been some discussion here whether it should be 4,5,6, or 7microns, many thanks. Richard Edwards MRC TOXICOLOGY UNIT LEICESTER..U.K....... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Thu May 18 08:05:27 2006 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu May 18 08:05:34 2006 Subject: [Histonet] section thickness References: Message-ID: <001b01c67a7b$bbdd33c0$a1065486@auxs.umn.edu> I do mine at 4 um. Jan Shivers UMN VDL ----- Original Message ----- From: "Edwards, R.E." To: Sent: Thursday, May 18, 2006 5:43 AM Subject: [Histonet] section thickness In your invaluable, but, no doubt differing opinions, what is the optimal section thickness for formalin fixed paraffin processed tissues for immunostaining, using DAB as chromogen?, there has been some discussion here whether it should be 4,5,6, or 7microns, many thanks. Richard Edwards MRC TOXICOLOGY UNIT LEICESTER..U.K....... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From yeepengtiang <@t> hotmail.com Thu May 18 08:13:05 2006 From: yeepengtiang <@t> hotmail.com (tiang yeepeng) Date: Thu May 18 08:13:11 2006 Subject: [Histonet] bleaching problem Message-ID: Dear all, I would like to ask if anyone ever experienced fast bleaching of IF staining of your samples after the exposure to light emitted from the fluorescence microscope. I am staining with a FITC conjugated secondary Ab (Jackson Immunolab) and mounted with ProLong Gold (Molecular Probes). The fluorescence got very dim after 20-30sec of exposure. Though I got back the signal after removing it from the fluorescence microscope awhile but the signal was very much weaker from the very first time I saw in the microscope. I am unable to find out what is the cause. Can anyone please advise? Thanks alot! With regards, TIANG YEE PENG Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Malaysia _________________________________________________________________ It?s the future of Hotmail: Try Windows Live Mail beta http://www2.imagine-msn.com/minisites/mail/Default.aspx?locale=en-us From POWELL_SA <@t> Mercer.edu Thu May 18 08:53:01 2006 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Thu May 18 08:53:55 2006 Subject: [Histonet] Georgia Society for Histotechnology In-Reply-To: <01M2ARWEZSA08X4MOD@Macon2.Mercer.edu> Message-ID: <01M2KAAJVVRY8WW02B@Macon2.Mercer.edu> Reminder:::: The Georgia Society for Histotechnology and the Georgia Society of Cytology will hold their 4th Annual Combined Scientific Symposium on Saturday, June 3, 2006, in Augusta, Georgia at the Marriott Augusta Hotel and Suites, 2 Tenth Street, Augusta, GA 30901. The phone number for reservations is 1-706 722-8900. I will email you a program upon request. Shirley Powell GSH Secretary/Membership Chair _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Thu May 18 09:21:43 2006 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu May 18 09:22:27 2006 Subject: [Histonet] Continuous process Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C63038BE088@EMAIL.archildrens.org> If you do a search on "LEAN histology" you will find some very interesting articles about histo labs who have done this. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: Thursday, May 18, 2006 1:55 AM To: 'Karnes, Lori SktnHR'; histonet@pathology.swmed.edu Subject: RE: [Histonet] Continuous process This is an interesting question; I contemplated continuous processing when I was a Cell Path Manager, but for the 'average' Histo Lab what would be the benefits? I guess if you concatenated a few middle sized Labs (?3) you could move to 24/7 and have a Hub Lab just like Cytology and LBC. The wild cards (as ever) would be the Pathologists; bit like hamsters IMHO. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 The past is never dead, it is not even past. --William Faulkner This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From scoop <@t> mail.nih.gov Thu May 18 09:55:12 2006 From: scoop <@t> mail.nih.gov (Sharon Cooperman) Date: Thu May 18 09:55:22 2006 Subject: [Histonet] procaine for perfusion Message-ID: Dear Histonetters, Does anyone use procaine in the wash in mouse perfusion to relax the vasculature and, if so, what concentration? Thanks, Sharon -- Sharon Cooperman NIH, NICHD, CBMB 301.435-8417 Building 18T, room 101 301.402-0078 fax Bethesda, MD 20892 From gu.lang <@t> gmx.at Thu May 18 10:32:51 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu May 18 10:32:52 2006 Subject: [Histonet] Ultraview Ventana Message-ID: <000301c67a90$533aa770$eeeea8c0@SERVER01> Hi ventana-users! Can anyone give me recommendations about the new multimer ultra-view kit of Ventana? Thank you Gudrun Lang Histolab Akh Linz, Austria From KParker <@t> mail.nih.gov Thu May 18 10:54:58 2006 From: KParker <@t> mail.nih.gov (Tuttle, Kimberly (NIH/NCI) [E]) Date: Thu May 18 10:55:05 2006 Subject: [Histonet] National Institutes: NIA or NIDA Message-ID: Do any of you know of anyone that works for National Institute on Aging (NIA) or the National Institute on Drug Abuse (NIDA)? If so can you send me their contact information? Kimberly C. Tuttle H.T.,(ASCP) NCI Center for Cancer Research Tissue Array Research Program http://resresources.nci.nih.gov/tarp From delventh3 <@t> aol.com Thu May 18 10:56:42 2006 From: delventh3 <@t> aol.com (delventh3@aol.com) Date: Thu May 18 10:56:57 2006 Subject: [Histonet] disinfecting the cryostat Message-ID: <8C848AACAB717FA-C10-7FE6@mblk-r13.sysops.aol.com> Does anyone have a protocol for disinfecting the cryostat? Specifically a substance that is specific for AFB. If you could give a call to anyone of us at the Histology Lab we would appreciate it. Thanks in advance. Priscilla Histology, St. Anthony's Hospital, Rockford, Ill. 815-395-3410 From jqb7 <@t> cdc.gov Thu May 18 10:40:49 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/NCID/VR)) Date: Thu May 18 10:59:10 2006 Subject: [Histonet] M. leprae and Nocardia specials Message-ID: Hi everyone: Can you please share with me which procedures you routinely use for M. leprae and Nocardia demonstration? I know most labs use 2 different procedures and I would like to know what they use. Thanks, Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov From pathrm35 <@t> adelphia.net Thu May 18 11:22:08 2006 From: pathrm35 <@t> adelphia.net (pathrm35@adelphia.net) Date: Thu May 18 11:22:12 2006 Subject: [Histonet] histotechnologists positions in Florida Message-ID: <8791073.1147969328672.JavaMail.root@web26> Fellow Techs, I have been hired to supervise a new dermpath lab in Florida. We are seeking two (good) techs for:routine histology techniques, IHC, special stains and grossing of skin biopsies.The reqiurements for these two positions are: at least a 2 year degree in a science as mandated by CLIA to gross, a Florida Histology Technologist license (not Technician), HT or HTL (ASCP), QIHC is a plus, and at least 3 years experience. Derm experience is a plus. This is a brand new lab with all new equipment. Day shift, M-F position unless you are interested in a second or third shift. Please contact me for more information. Ron Martin From Barry.R.Rittman <@t> uth.tmc.edu Thu May 18 11:38:49 2006 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Thu May 18 11:38:54 2006 Subject: [Histonet] section thickness Message-ID: This is a difficult question to answer as it depends on exactly what you wish to measure. In general The thinner the sections the more precisely you can locate and measure the reaction product but the fewer entire cells will be seen. If you are counting cells then the thicker the sections and the more localized the reaction product the better as otherwise you should apply a correction factor (as for example per Abercrombie 1946). This is because in image analysis of thinner sections it is often difficult to set limits to exclude partial cells. Another point to consider is that of determining the section thickness. Most assume that if you cut two blocks at 4 micron section thickness that you will be able to compare the sections for density of reaction product. However we all know that it is difficult to get all sections of the same exact thickness. If one is at 4 microns and the next at 3 microns then the second has 25% less volume of tissue and presumably will have less total reaction product. There are steps that can be taken to minimize or eliminate this error such as including a material of know optical density in the blocks that are cut along with the tissue. Hope that these comments are useful. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E. Sent: Thursday, May 18, 2006 5:44 AM To: histonet@pathology.swmed.edu Subject: [Histonet] section thickness In your invaluable, but, no doubt differing opinions, what is the optimal section thickness for formalin fixed paraffin processed tissues for immunostaining, using DAB as chromogen?, there has been some discussion here whether it should be 4,5,6, or 7microns, many thanks. Richard Edwards MRC TOXICOLOGY UNIT LEICESTER..U.K....... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Thu May 18 11:50:05 2006 From: shive003 <@t> umn.edu (Jan Shivers) Date: Thu May 18 11:50:40 2006 Subject: [Histonet] IHC - Mycoplasma Ab Message-ID: <008801c67a9b$1d6b03f0$a1065486@auxs.umn.edu> Does anyone know where I might find an unconjugated antibody to Mycoplasma hyopneumoniae? Thanks in advance. Jan Shivers UMN Vet Diag Lab IHC/Histo/EM shive003@umn.edu From liz <@t> premierlab.com Thu May 18 11:59:55 2006 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu May 18 12:00:08 2006 Subject: [Histonet] rabbit anti-FITC Message-ID: <000001c67a9c$7e6a7270$0300a8c0@Chlipala> Hello everyone I was searching the histonet archives and came across an e-mail from Gayle Callis about the use of Dako's rabbit anti-FITC antibody for FITC labeled mouse antibodies on mouse tissue. To make a long story short, it seems that Dako has discontiued this item, is anyone aware of a good subsititue for this, the one I was interested in was not biotinylated. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 From tmhpath <@t> amigo.net Thu May 18 12:01:43 2006 From: tmhpath <@t> amigo.net (Michelle D. Moore) Date: Thu May 18 12:01:50 2006 Subject: [Histonet] Help workers comp issue Message-ID: <000601c67a9c$bde339f0$ef00a8c0@tmhpath1> Hello fellow colleagues, I am at a point of frustration with workers comp, some surprise there huh? I need to be pointed in the direction of getting information about histotechs and repetitive motion disorders. I have a tear on my tendon in my right elbow with epicondylitis and bilateral carpal tunnel syndrome and of course they are saying it is not work related (BULL). I did not have this problem until working in histology and I do not play any sports or anything that would cause these problems. I would really appreciate some help with this as I am going to have to get a lawyer to even get anything done about this. I appreciate all your time and help with this. Sorry to vent but I just got off the phone with workers comp and I am angry and hurt. Thank you again for your time and help. Michelle D. Moore HT(ASCP) The Memorial Hospital 785 Russell St Craig, CO 81625 tmhpath@amigo.net 970-826-3292 Fax 970-826-3158 From Jackie.O'Connor <@t> abbott.com Thu May 18 12:19:47 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu May 18 12:20:18 2006 Subject: [Histonet] Help workers comp issue In-Reply-To: <000601c67a9c$bde339f0$ef00a8c0@tmhpath1> Message-ID: Michelle - Speaking as someone who has suffered from tendinitis from microtomy, who says it's not work related? Workmans comp? What is your claim for? Missed work, health care costs, or other damages? What does your doctor say? You'll need supporting documentation from your ortho for any action to be taken seriously. It sounds as if your place of employment isn't cooperating - I slammed my finger in a drawer at work a couple of months ago, and did some nerve damage - I didn't go to employee health until a week later because I thought it was stupid, but then they were all over it - even sent me to a hand specialist. The feeling eventually did come back in my finger (I can tell, you were ALL worried) - anyway - it's basically the doctor's call - and he'll recommend how to prevent additional damage, which probably includes adapting your microtomy style, among other things. Then you probably could get a good personal injury lawyer who will do all the research for 30% of your settlement. Good luck. "Michelle D. Moore" Sent by: histonet-bounces@lists.utsouthwestern.edu 05/18/2006 12:01 PM To "Histonet" cc Subject [Histonet] Help workers comp issue Hello fellow colleagues, I am at a point of frustration with workers comp, some surprise there huh? I need to be pointed in the direction of getting information about histotechs and repetitive motion disorders. I have a tear on my tendon in my right elbow with epicondylitis and bilateral carpal tunnel syndrome and of course they are saying it is not work related (BULL). I did not have this problem until working in histology and I do not play any sports or anything that would cause these problems. I would really appreciate some help with this as I am going to have to get a lawyer to even get anything done about this. I appreciate all your time and help with this. Sorry to vent but I just got off the phone with workers comp and I am angry and hurt. Thank you again for your time and help. Michelle D. Moore HT(ASCP) The Memorial Hospital 785 Russell St Craig, CO 81625 tmhpath@amigo.net 970-826-3292 Fax 970-826-3158 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gpbnas <@t> yahoo.es Thu May 18 13:00:41 2006 From: gpbnas <@t> yahoo.es (Guillermo Palao) Date: Thu May 18 13:00:45 2006 Subject: [Histonet] Good antibodies for macrophage detection in rat samples Message-ID: <20060518180041.17105.qmail@web26206.mail.ukl.yahoo.com> Hello all, We are interested in detecting macrophages in FFPE samples from rat. I would really appreciate any suggestions regarding the best antigen (Is F4-80 the best?) and most useful commercial antibody to use. Thanks in advance, Guillermo --------------------------------- LLama Gratis a cualquier PC del Mundo. Llamadas a fijos y m?viles desde 1 c?ntimo por minuto. http://es.voice.yahoo.com From VAZQUEZR <@t> ohsu.edu Thu May 18 13:00:46 2006 From: VAZQUEZR <@t> ohsu.edu (Robyn Vazquez) Date: Thu May 18 13:01:28 2006 Subject: [Histonet] disinfecting the cryostat Message-ID: We use a product that is a broad-spectrum, SaniMaster IV Google it. Shut cryo down, drench inside with diluted SaniMaster IV, let set for awhile, drain, wipe out, spray with 100%, wipe out, air dry for awhile, plug back in, wah la...tried calling number, but got a not in service announcement. Hope this helps Robyn OHSU >>> 5/18/2006 8:56 AM >>> Does anyone have a protocol for disinfecting the cryostat? Specifically a substance that is specific for AFB. If you could give a call to anyone of us at the Histology Lab we would appreciate it. Thanks in advance. Priscilla Histology, St. Anthony's Hospital, Rockford, Ill. 815-395-3410 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Thu May 18 13:09:17 2006 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu May 18 13:09:28 2006 Subject: [Histonet] Good antibodies for macrophage detection in rat samples In-Reply-To: <20060518180041.17105.qmail@web26206.mail.ukl.yahoo.com> Message-ID: <000701c67aa6$2df0e680$0300a8c0@Chlipala> F4/80 is a murine macrophage marker. I use ED-1 from serotec, it?s a mouse anti-rat macrophage marker. It works well on FFPE tissue with proteinase K digestion, (even works on formic acid decalcifed bone). Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Guillermo Palao Sent: Thursday, May 18, 2006 12:01 PM To: Histonet Subject: [Histonet] Good antibodies for macrophage detection in rat samples Hello all, We are interested in detecting macrophages in FFPE samples from rat. I would really appreciate any suggestions regarding the best antigen (Is F4-80 the best?) and most useful commercial antibody to use. Thanks in advance, Guillermo --------------------------------- LLama Gratis a cualquier PC del Mundo. Llamadas a fijos y m?viles desde 1 c?ntimo por minuto. http://es.voice.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SHARON.OSBORN <@t> SPCORP.COM Thu May 18 13:17:13 2006 From: SHARON.OSBORN <@t> SPCORP.COM (Osborn, Sharon) Date: Thu May 18 13:18:17 2006 Subject: [Histonet] section thickness for IHC Message-ID: <9A919A5D70313A4D9C56A025710874080C72B2@kenmsg40.us.schp.com> We do ours at 5 microns, brains, CNS are at 10-15 microns sharon osborn DNAX, SP BioPharma Palo Alto, CA Subject: [Histonet] section thickness In your invaluable, but, no doubt differing opinions, what is the optimal section thickness for formalin fixed paraffin processed tissues for immunostaining, using DAB as chromogen?, there has been some discussion here whether it should be 4,5,6, or 7microns, many thanks. Richard Edwards MRC TOXICOLOGY UNIT LEICESTER..U.K. ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From omnivore98 <@t> yahoo.com Thu May 18 13:45:14 2006 From: omnivore98 <@t> yahoo.com (Heather Renko) Date: Thu May 18 13:45:18 2006 Subject: [Histonet] specimen size for IHC Message-ID: <20060518184514.12226.qmail@web31305.mail.mud.yahoo.com> The optimum size for IHC is 3-4mm thick and 1-2cm square to allow adequate penetration in the fixation process. The Immunochemical Staining handbook(3rd Ed, published by Dako) states to cut at 4-6. Heather Renko In your invaluable, but, no doubt differing opinions, what is the optimal section thickness for formalin fixed paraffin processed tissues for immunostaining, using DAB as chromogen?, there has been some discussion here whether it should be 4,5,6, or 7microns, many thanks. Richard Edwards --------------------------------- Ring'em or ping'em. Make PC-to-phone calls as low as 1?/min with Yahoo! Messenger with Voice. From Jackie.O'Connor <@t> abbott.com Thu May 18 14:34:55 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu May 18 14:35:25 2006 Subject: [Histonet] specimen size for IHC In-Reply-To: <20060518184514.12226.qmail@web31305.mail.mud.yahoo.com> Message-ID: CM Van der Loos is the "Dr. House of Histology". I believe whatever he says unequivocally. I pulled this from the Jan 06 archives. [Histonet] RE: optimal thickness for cutting of IHC sections From: "C.M. van der Loos" Dear Paul, I totally agree what you wrote about IHC: it's just a surface event. Long time ago we had to prove that our (prehistoric) attempt of quantifying an IHC technique wasn't influenced by tissue thickness due to microtome abberations. We did cut (cryostat) sections of different thickness and guess what: there was hardly any influence on the staining intensity. The only thing we observed was that thicker sections showed a higher non-specific background staining than thinner sections. See: CM van der Lo os, MMH Marijianowski and AE Becker: Quantification in immunohistochemistry. The measurement of the ratio?s between collagen types I and I II. Histochem J (1994) 26, 347-354<= /P> Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M 2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Nether lands Date: Tue, 3 Jan 2006 13:09:20 -0500 From: "Monfils, Paul" Subject : RE: [Histonet] optimal thickness for cutting of IHC sections< BR>To: 'histonet@lists.utsouthwestern.edu' I believe section thickness is less critical for IHC because antibodies are very large molecules that don't penetrate tissue very well, so regardless of the thickness of the section, you are really only staining the exposed surface of the tissue, perhaps to a depth of 2 microns or so. We have verified this by electron microscopy. This is quite different from standard histochemical procedures where a thicker section results in a more intense stain because the small dye molecules penetrate the tissue readily and stain it all the way through. Heather Renko Sent by: histonet-bounces@lists.utsouthwestern.edu 05/18/2006 01:45 PM To histonet@pathology.swmed.edu cc Subject [Histonet] specimen size for IHC The optimum size for IHC is 3-4mm thick and 1-2cm square to allow adequate penetration in the fixation process. The Immunochemical Staining handbook(3rd Ed, published by Dako) states to cut at 4-6. Heather Renko In your invaluable, but, no doubt differing opinions, what is the optimal section thickness for formalin fixed paraffin processed tissues for immunostaining, using DAB as chromogen?, there has been some discussion here whether it should be 4,5,6, or 7microns, many thanks. Richard Edwards --------------------------------- Ring'em or ping'em. Make PC-to-phone calls as low as 1?/min with Yahoo! Messenger with Voice. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From psicurello <@t> mcvh-vcu.edu Thu May 18 15:16:24 2006 From: psicurello <@t> mcvh-vcu.edu (Paula Sicurello) Date: Thu May 18 15:19:16 2006 Subject: [Histonet] Histotech Position in Richmond, VA Message-ID: Hello Fellow Histologists, The Medical College of Virgini histotechnologists. We have 2 hi The posting only shows the one position as yet, but there will be 2 jobs. Please go to [1]www.vcuhealth.org and look under the Allied Health section of the job listings. It lists the hours but I don't know how set those are. If you're interested, you can e-mail me a copy of your resume and I'll pass it along to the powers that be. Also, please fill out the o Thanks to all who are interested, Paula :-) References 1. 3D"http://www.vcuhealth.org"=/ From liz <@t> premierlab.com Thu May 18 15:23:47 2006 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu May 18 15:23:57 2006 Subject: [Histonet] rabbit anti-GFP FITC labeled Message-ID: <000601c67ab8$f7d4d710$0300a8c0@Chlipala> Hello Does anyone out there know of a good rabbit anti-GFP that FITC labeled. The client I'm working with wants an affinity purified antibody if possible. Thanks in advance. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 From anh2006 <@t> med.cornell.edu Thu May 18 15:59:15 2006 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Thu May 18 15:58:26 2006 Subject: [Histonet] RE: immunofluorescence amplification... In-Reply-To: References: Message-ID: I find using a biotinylated secondary and Streptavidin labeled with fluorophore also works well for amplication, certainly a step up from directly labeled secondaries where amplification is desired. Good luck! Andrea At 8:56 AM +0200 5/17/06, C.M. van der Loos wrote: > > > Date: Mon, 15 May 2006 16:44:28 -0700 (PDT) > From: Adam Perry > Subject: [Histonet] immunofluorescence amplification... > To: histonet@pathology.swmed.edu > What are people's impressions about different (i.e. the best) ways to > amplify fluorescent signal from immunocytochemistry? > I'm working with 30 um rodent brain sections. When I use a > biotinylated secondary antibody with avidin-biotin-HRP detection with > DAB or NiDAB I get good cellular staining with a primary antibody > concentration of 1:1,000. > I am now trying to perform double labeling and so have switched to > fluorescence...and I don't seem to be getting any specific signal > now. > I have tried increasing the antibody concentration from 1:1,000 up to > 1:100 and still don't see the faintest hint of staining (and the > background just gets really bad). > I've used the fluorescent antibody to detect other primaries (so the > secondary is working in general and my mi! croscope >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- From RRA <@t> Stowers-Institute.org Thu May 18 16:45:27 2006 From: RRA <@t> Stowers-Institute.org (Allen, Rhonda) Date: Thu May 18 16:45:53 2006 Subject: [Histonet] FW: Jb-4 blocks Message-ID: > Histonetter's, > > The following question was posted on the microscopy list serve by a > friend. I thought maybe some of the folks in histoland might have > some good suggestions. > > Rhonda Allen > > > "We are doing a rush job for a client who requires 4.0 um sections > from JB 4 blocks. Our ultramicrotomists extraordinaire, Cheryl, is > having a dickens of a time getting the sections to remain flat when > removing them from the knife. She is cutting on glass and taking > sections from the dry edge with a fine forceps. As soon as the > sections leave the knife, they curl and won't uncurl when placed on a > drop of water on a slide. > > Not only is this a rush job in support of a grant proposal, but it > requires serial sectioning with no missing sections, and we have, > like, > no real experience with this resin. Cheryl has tried various sized > block faces and different thicknesses for the sections, but nothing is > helping. > > The thumping sound you hear is a head hitting a wall----repeatedly. > Can anyone HEEEELLLLP??" > > From gcallis <@t> montana.edu Thu May 18 17:00:52 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu May 18 17:01:04 2006 Subject: Source of Re: [Histonet] IHC - Mycoplasma Ab In-Reply-To: <008801c67a9b$1d6b03f0$a1065486@auxs.umn.edu> References: <008801c67a9b$1d6b03f0$a1065486@auxs.umn.edu> Message-ID: <6.0.0.22.1.20060518155847.01b31680@gemini.msu.montana.edu> Richard Cartun gave out a fabulous source, ViroStat out of Portland Maine. Just go to ViroStat website and shop - Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From lrichey <@t> u.washington.edu Thu May 18 17:01:10 2006 From: lrichey <@t> u.washington.edu (Lori Richey) Date: Thu May 18 17:01:33 2006 Subject: [Histonet] Sakura Xpress users In-Reply-To: References: Message-ID: <446CEEA6.3090006@u.washington.edu> We use the xpress processor for most biopsy specimens like liver, cervical, prostate, and oral path. Our pathologists have not agreed to put the renal, skin biopsies or the breast biopsies on the express. We also still run all of the regular surgical specimens on the VIP. These work well on the xpress, however, they must be cut in at the recommended size limits. Also, due to the fixation time in formalin for most of the large specimens it works better to load them in the VIP in formalin for a couple hours before the processor runs. There are only 2 end chambers on the Xpress, meaning someone has to be present to unload it. Most of the special stains and the IHC staining is comparable to routine processing. The Trich stain on liver biopsies done on the Artisan special stainer has been the most challenging. Angela Bitting wrote: >I'd love to get opinions from hospital Histology labs using the Xpress processor. Our Director went off to "War College" and heard all about it and you all know what happens next............. What are the benefits and drawbacks to it? > >Angela Bitting, HT(ASCP) >Technical Specialist, Histology >Geisinger Medical Center >100 N Academy Ave. MC 23-00 >Danville, PA 17822 >phone 570-214-9634 >fax 570-271-5916 > > > >IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From gcallis <@t> montana.edu Thu May 18 17:10:05 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu May 18 17:10:20 2006 Subject: [Histonet] rabbit anti-FITC In-Reply-To: <000001c67a9c$7e6a7270$0300a8c0@Chlipala> References: <000001c67a9c$7e6a7270$0300a8c0@Chlipala> Message-ID: <6.0.0.22.1.20060518160705.01b39f00@gemini.msu.montana.edu> This is sad as this antibody worked so nicely. It worked beautifully with Rat antMouse CD4-FITC. Chris van der Loos was the one who clued me in to this antibody, as he used it cleverly for his double staining. Maybe he has a new source for one of equal quality. Gayle Callis At 10:59 AM 5/18/2006, you wrote: >Hello everyone > >I was searching the histonet archives and came across an e-mail from >Gayle Callis about the use of Dako's rabbit anti-FITC antibody for FITC >labeled mouse antibodies on mouse tissue. To make a long story short, >it seems that Dako has discontiued this item, is anyone aware of a good >subsititue for this, the one I was interested in was not biotinylated. > >Thanks in advance > >Liz > >Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC >Manager >Premier Laboratory, LLC >P.O. Box 18592 >Boulder, Colorado 80308 >Office: (303) 735-5001 >Fax: (303) 735-3540 >liz@premierlab.com >www.premierlab.com > >Ship to Address: >Premier Laboratory >University of Colorado >MCDB, Room A3B40 >Boulder, Colorado 80309 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Thu May 18 17:15:42 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu May 18 17:15:50 2006 Subject: [Histonet] Good antibodies for macrophage detection in rat samples In-Reply-To: <20060518180041.17105.qmail@web26206.mail.ukl.yahoo.com> References: <20060518180041.17105.qmail@web26206.mail.ukl.yahoo.com> Message-ID: <6.0.0.22.1.20060518161117.01b49008@gemini.msu.montana.edu> F4-80 ( a rat antimouse monoclonal) is for mouse macrophages, not rat. There are several well documented rat macrophage antibodies, go to Serotec and look for these. ED1, ED2, ED3 are subsets. These mouse antiRat monoclonals were detected with goat antimouse secondaries that mouse IgG isotypes (IgG1, etc) biotinylated from Southern Biotechnology. At 12:00 PM 5/18/2006, you wrote: >Hello all, > > We are interested in detecting macrophages in FFPE samples from rat. I > would really appreciate any suggestions regarding the best antigen (Is > F4-80 the best?) and most useful commercial antibody to use. > > Thanks in advance, > > Guillermo > > >--------------------------------- > >LLama Gratis a cualquier PC del Mundo. >Llamadas a fijos y m?viles desde 1 c?ntimo por minuto. >http://es.voice.yahoo.com >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From jqb7 <@t> cdc.gov Thu May 18 17:29:05 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/NCID/VR)) Date: Thu May 18 17:29:22 2006 Subject: [Histonet] Sakura Xpress users References: <446CEEA6.3090006@u.washington.edu> Message-ID: Lori: What is the conflict with the pathologists regarding the renal, skin and breast biopsies being run on the Xpress? Thanks. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Lori Richey Sent: Thu 5/18/2006 6:01 PM To: Angela Bitting Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Sakura Xpress users We use the xpress processor for most biopsy specimens like liver, cervical, prostate, and oral path. Our pathologists have not agreed to put the renal, skin biopsies or the breast biopsies on the express. We also still run all of the regular surgical specimens on the VIP. These work well on the xpress, however, they must be cut in at the recommended size limits. Also, due to the fixation time in formalin for most of the large specimens it works better to load them in the VIP in formalin for a couple hours before the processor runs. There are only 2 end chambers on the Xpress, meaning someone has to be present to unload it. Most of the special stains and the IHC staining is comparable to routine processing. The Trich stain on liver biopsies done on the Artisan special stainer has been the most challenging. Angela Bitting wrote: >I'd love to get opinions from hospital Histology labs using the Xpress processor. Our Director went off to "War College" and heard all about it and you all know what happens next............. What are the benefits and drawbacks to it? > >Angela Bitting, HT(ASCP) >Technical Specialist, Histology >Geisinger Medical Center >100 N Academy Ave. MC 23-00 >Danville, PA 17822 >phone 570-214-9634 >fax 570-271-5916 > > > >IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Thu May 18 17:36:17 2006 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Thu May 18 17:35:33 2006 Subject: [Histonet] rabbit anti-FITC In-Reply-To: <6.0.0.22.1.20060518160705.01b39f00@gemini.msu.montana.edu> References: <000001c67a9c$7e6a7270$0300a8c0@Chlipala> <6.0.0.22.1.20060518160705.01b39f00@gemini.msu.montana.edu> Message-ID: Bummer, I loved this antibody also for mouse on mouse staining. DAKO sells a mouse anti-FITC, clone DAK-FITC4, which is excellent for staining human tissue with FITC labeled antibodies using the Mouse EnVision+ kit as the detection system. I am hoping this antibody was not discontinued as well! Also FYI, there is a sheep anti-FITC-HRP (from the TUNEL POD kit but sold separately also) from Roche Molecular Biochemicals which is excellent and I routintely use it for conversion of good strong labeling FITC antibodies to HRP/DAB detection. (The primary must obviously stain robustly since there is a lack of amplification using this protocol). With just a quick scan of InVitrogen/Zymed/Molecular Probes website .... I see a rabbit anti-FITC from Zymed Catalog #71-1900, I know nothing about its quality though, probably decent! -- Andrea >At 10:59 AM 5/18/2006, you wrote: >>Hello everyone >> >>I was searching the histonet archives and came across an e-mail from >>Gayle Callis about the use of Dako's rabbit anti-FITC antibody for FITC >>labeled mouse antibodies on mouse tissue. To make a long story short, >>it seems that Dako has discontiued this item, is anyone aware of a good >>subsititue for this, the one I was interested in was not biotinylated. >> >>Thanks in advance >> >>Liz >> >>Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC >>Manager >>Premier Laboratory, LLC >>P.O. Box 18592 >>Boulder, Colorado 80308 >>Office: (303) 735-5001 >>Fax: (303) 735-3540 >>liz@premierlab.com >>www.premierlab.com -- From BSylinda <@t> aol.com Thu May 18 18:27:37 2006 From: BSylinda <@t> aol.com (BSylinda@aol.com) Date: Thu May 18 18:27:44 2006 Subject: [Histonet] Her 2 question Message-ID: <3e5.2d9bf9d.319e5ce9@aol.com> Hello Histoland, Can anyone out there can give me some info about if you are using a multi control for Her 2. We currently send out Her 2, and we are getting a single 1 punch control equal to a 3 plus control. My question is how do you run a 3 plus control in house and without running a multi control which has 0, 1+, and 3+. All feedback will be greatly appreciated, Thanks in advance Sylinda Battle HT ASCP From katri <@t> cogeco.ca Thu May 18 20:30:34 2006 From: katri <@t> cogeco.ca (Katri Tuomala) Date: Thu May 18 20:30:29 2006 Subject: [Histonet] section thickness References: Message-ID: <002b01c67ae3$d35adcb0$6a9a9618@Katri> Richard, I do all my immunos at 3 um, always have for the last 20 years... Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "Edwards, R.E." To: Sent: Thursday, May 18, 2006 6:43 AM Subject: [Histonet] section thickness In your invaluable, but, no doubt differing opinions, what is the optimal section thickness for formalin fixed paraffin processed tissues for immunostaining, using DAB as chromogen?, there has been some discussion here whether it should be 4,5,6, or 7microns, many thanks. Richard Edwards MRC TOXICOLOGY UNIT LEICESTER..U.K....... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BNuern <@t> coj.net Thu May 18 20:45:24 2006 From: BNuern <@t> coj.net (Barb Nuernberger) Date: Thu May 18 20:45:43 2006 Subject: [Histonet] Please unsubscibe me from your list server. Thank you. Message-ID: Please unsubscibe me from your list server. Thank you. Barb Nuernberger From vanamalajl <@t> yahoo.com Thu May 18 21:23:39 2006 From: vanamalajl <@t> yahoo.com (Jairam Vanamala) Date: Thu May 18 21:23:46 2006 Subject: [Histonet] Beta-catenin Message-ID: <20060519022339.56541.qmail@web31806.mail.mud.yahoo.com> Does anyone has the working IHC protocol for beta-catenin, preferably for rat colon. Jairam Vanamala Post-doc TAMU College Station, TX __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From drgeoffreywhite <@t> yahoo.com Fri May 19 07:52:10 2006 From: drgeoffreywhite <@t> yahoo.com (Geoffrey White) Date: Fri May 19 07:52:13 2006 Subject: [Histonet] Incubation chamber Message-ID: <20060519125210.64906.qmail@web38107.mail.mud.yahoo.com> Try out QInstruments. There you will find a new incubation chamber for a little price. Good Luck Geoffrey __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From David.Edmondson <@t> christie-tr.nwest.nhs.uk Fri May 19 08:35:44 2006 From: David.Edmondson <@t> christie-tr.nwest.nhs.uk (Edmondson David (RBV) NHS Christie Tr) Date: Fri May 19 08:35:54 2006 Subject: [Histonet] FW: Jb-4 blocks Message-ID: <3C83687E8F6AE04792E361ABE2D385B8418115@cht-mail2-2k.xchristie.nhs.uk> Hi, It's years ago, but I remember something along the lines of 70% alc, and picking up the section with a brush in a droplet of the alcohol and flicking it onto a staining pot of water. They span and the creases came out . Then picked up onto slides and dried on a hotplate. Does that approach sound familiar to anyone? David Christie Hosp Manchester UK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Allen, Rhonda Sent: 18 May 2006 22:45 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Jb-4 blocks > Histonetter's, > > The following question was posted on the microscopy list serve by a > friend. I thought maybe some of the folks in histoland might have > some good suggestions. > > Rhonda Allen > > > "We are doing a rush job for a client who requires 4.0 um sections > from JB 4 blocks. Our ultramicrotomists extraordinaire, Cheryl, is > having a dickens of a time getting the sections to remain flat when > removing them from the knife. She is cutting on glass and taking > sections from the dry edge with a fine forceps. As soon as the > sections leave the knife, they curl and won't uncurl when placed on a > drop of water on a slide. > > Not only is this a rush job in support of a grant proposal, but it > requires serial sectioning with no missing sections, and we have, > like, > no real experience with this resin. Cheryl has tried various sized > block faces and different thicknesses for the sections, but nothing is > helping. > > The thumping sound you hear is a head hitting a wall----repeatedly. > Can anyone HEEEELLLLP??" > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************** This e-mail and any files transmitted with it are confidential and solely for the use of the intended recipient. If you have received this e-mail in error you should not disseminate, distribute or copy it. Please notify the sender immediately and delete this e-mail from your system. ******************************************************************************************************************* From cmjohnson <@t> cmh.edu Fri May 19 08:57:32 2006 From: cmjohnson <@t> cmh.edu (Johnson, Cindy, M) Date: Fri May 19 08:57:43 2006 Subject: [Histonet] unsubscribe Message-ID: Please unsubscribe me from your list server. Thank you. From ashokc <@t> ncbs.res.in Fri May 19 09:16:09 2006 From: ashokc <@t> ncbs.res.in (ashokc@ncbs.res.in) Date: Fri May 19 09:32:55 2006 Subject: [Histonet] (no subject) Message-ID: <1249.59.92.206.50.1148048169.squirrel@59.92.206.50> please unsubscribe from histonet Ashok.C Research Scholar Prof.M.M.Panicker's lab Molecular Neurobiology National Centre for Biological Sciences GKVK Campus Bangalore-560065 Phone-+91-080-23636421 ext :2251 Fax no-91-080-23636662 From PatPatterson <@t> mhd.com Fri May 19 09:52:59 2006 From: PatPatterson <@t> mhd.com (Patterson, Pat) Date: Fri May 19 09:52:41 2006 Subject: [Histonet] Histology Position Message-ID: <293C7C19EFF7D611AE1A0002A53F8114164AC225@omega.mhd.com> Methodist Dallas Medical Center - voted "One of the Best Places to Work in Dallas" currently has a full-time 2nd shift position available for a certified Histology Technician/Technologist. Experience in all aspects of Histology are required. Frozen section and Immunofluoresence experience is desired. We also have a PRN position available for either 1st or 2nd shift hours. Contact : Pat Patterson 214-947-3538 patpatterson@mhd.com OR Lorena Davila, HR recruiter lorenadavila@mhd.com *********************************************************************** This electronic transmission contains information from Methodist Health System and should be considered confidential and privileged. The information contained in the above messages is intended only for the use of the individual(s) and entity(ies) named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution, or use of this information is prohibited. If you receive this transmission in error, please notify the sender immediately by return e-mail. Methodist Health System, its subsidiaries and affiliates hereby claim all applicable privileges related to the transmission of this communication. From rodney.collins <@t> ucsf.edu Tue May 16 09:51:09 2006 From: rodney.collins <@t> ucsf.edu (Rodney Collins) Date: Fri May 19 10:10:58 2006 Subject: [Histonet] Whole human coronal brain sections in Paraffin Message-ID: Whole human coronal brain sections in Paraffin: Our lab is looking for a courageous histotech who can cut paraffin sections of human whole coronal slices. Initially we have 3 practice blocks but eventually we will have approximately 30 large blocks and will need 4 sections per block. We are the Neuropath department at UCSF. My Name is Rodney and you can reach me directly at 415 476-1194 or by email at Rodney.Collins@ucsf.edu From jkiernan <@t> uwo.ca Wed May 17 23:42:48 2006 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Fri May 19 10:11:00 2006 Subject: [Histonet] Bouin's alternative/substitution question - Rather long reply In-Reply-To: References: Message-ID: <446BFB48.2000402@uwo.ca> Dear Jason, After 6 days nobody has posted a Histonet answer to your two questions. This is not surprising. The product "Bouin's fixative substitute" is on the vendor's website, and elsewhere on the Web too, but with no indication of what it contains. Without this knowledge, who could take the risk of using the product? Bouin's fixative contains three ingredients, each with a function that has been fairly well understood since Bouin published the recipe in 1897. The advantages and limitations of Bouin's fluid are also well documented, and are familiar to anyone who examines Bouin-fixed and otherwise-fixed preparations. If the substitute does not contain formaldehyde or picric acid, what chemicals replace the actions of both these compounds? I think we should be told! Davidson's fixative, mentioned in one reply, is one of several AFA or FAA (alcohol-formalin-acetic) mixtures, with an alcohol content (30%) lower than most others in this class. It has an interesting and incompletely known history; evidently Davidson never published the mixture himself. Check out the Histosearch archives; also http://members.aol.com/RSRICHMOND/histology.html Another use of Bouin's fluid is pre-treating sections of formaldehyde-fixed tissue before staining by a trichrome method (Masson, Mallory, Heidenhain, Cason, Gomori, Gabe etc). The pre-treatment enhances the different coloration of collagen fibres and cytoplasm (especially that of smooth muscle). This effect is equally achievable with an aqueous solution of picric acid. Other compounds might achieve the same effect, but have their advantages passed the test of peer reviewed publication? Nothing in an AFA/Davidson mixture has this priming action for trichrome stains. For what it's worth, my advice is to use Bouin's fluid as a fixative (if you are happy with the results). If you have to do nucleic acid histochemistry, change the routine fixative to neutral buffered formaldehyde, an AFA or even a simple formaldehyde-free alcohol-acetic mixture such as Clarke's or Carnoy's. For really critical work requiring preservation of intracellular structure at the oil-immersion level of resolution in paraffin sections it is difficult to avoid mercuric chloride, osmium tetroxide and potassium dichromate, if organelles and thin collagenous structures are to be stained with traditional methods that use dyes. For immunohistochemistry, HgCl2, OsO4 and K2Cr2O7 are best avoided, but picric acid has a long and honourable history of being IHC-friendly. (This may have nothing to do with its fixative action. I could expound but won't.) Bottom 2 lines: 1. Know your fixative, and don't use any mixture with undisclosed ingredients. 2. A wrong diagnosis could easily result from inappropriate fixation. If this comes to judgement: a pathologist might be scolded, but a technician (less expensive lawyer) is likely to be fired (scapegoat). John Kiernan Anatomy, UWO London, Canada --------------------------------------- jason.burrill@crl.com wrote: > > We are removing Bouin's fixative from our laboratory and we are looking > for an alternative. Has anyone had experience in using the Bouin's > Fixative Substitution distributed by IMEB or something similar from > another vendor? From reviewing the Histonet archives I see that most > people use Davidson's fixative but I just wanted to see if anyone had any > personal experience with these substitutions. > Thanks in advance, > Jason > > Jason D. Burrill > Manager, Histology > Charles River Laboratories > 251 Ballardvale Street > Wilmington, MA 01887 > ph: 978-658-6000 ext 1652 > fax: 978-988-8793 > E-mail: jason.burrill@crl.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ccrowder <@t> vetmed.lsu.edu Thu May 18 14:41:47 2006 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Fri May 19 10:11:03 2006 Subject: [Histonet] M. leprae staining Message-ID: Jeanine - Our lab works closely with the labs of the National Hansen's Disease Center. We modified their protocol and published it in the JOH, June 1996, Vol 19 #2. It's very easy, takes about 45 minutes and have little or no background. If you do not have a copy of the journal, I contact me directly and I'll fax you the directions. Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 From Kelly.Stanley <@t> cchmc.org Thu May 18 14:52:28 2006 From: Kelly.Stanley <@t> cchmc.org (Kelly Stanley) Date: Fri May 19 10:11:04 2006 Subject: [Histonet] BEST FIXATIVE FOR BRAIN TISSUE&In-Reply-To= Message-ID: Hello, I too am looking for the best fixative for brain. Did you find any more information?? Or has your search ended. Thank-You Kelley HAVE A GOOD DAY KELLEY STANLEY From Sharon.Genest <@t> saskatoonhealthregion.ca Fri May 19 10:57:19 2006 From: Sharon.Genest <@t> saskatoonhealthregion.ca (Genest, Sharon SktnHR) Date: Fri May 19 10:57:39 2006 Subject: [Histonet] Bar-coding and interfacing cassette and slide printers. Message-ID: <1C152FCB03A4F84197818DEE847F0D4A264E94@stampy.sktnhr.ca> I am currently working on a project to barcode all Surgical pathology and Cytology specimens with the hopes of eventually going paperless. At the same time we are investigating the possibility of auto generating cassettes and slides for surgical pathology and non gyne cytology specimens, and labels for gyne (PAP) slides. My questions are: 1 Is anyone using an interfaced cassette labeller or slide labeller or both, that could share with me information good or bad on how what they use and how it is working for them. 2 Is anyone auto generating cassettes during accessioning? 3 Is anyone using a slide printing system that they do or could share with cytology? Any help would be appreciated. Thanks in advance. From ian.montgomery <@t> bio.gla.ac.uk Fri May 19 11:50:31 2006 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Fri May 19 11:50:41 2006 Subject: [Histonet] Tadpoles Message-ID: <009701c67b64$576942a0$532ed182@ibls.gla.ac.uk> Student going to Trinidad this summer where she will study frogs. Most of us would simply chill and drink rum but this is a keen young student. Any recommendations for a fixative for tadpoles just as they are about too emerge from the egg. No jelly, just a tadpole inside the egg. Ian. Dr. Ian Montgomery, Histotechnology, IBLS Support Unit, Graham Kerr Building, Tel: 4652, 6644. ian.montgomery@bio.gla.ac.uk From rjbuesa <@t> yahoo.com Fri May 19 12:03:14 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri May 19 12:03:21 2006 Subject: [Histonet] Tadpoles In-Reply-To: <009701c67b64$576942a0$532ed182@ibls.gla.ac.uk> Message-ID: <20060519170314.93652.qmail@web61211.mail.yahoo.com> Ian: After finding "the hard way" that the worst fixative for this type of specimen is the picro-acetic-formaldhyde of Bouin, I sucessfully used the one developed by Gregg and Puckett (1943): water 225 mL + 40% formaldehyde 20 mL + acetic acid 5 mL and mercuric chloride 12.5 g To avoid merury bichloride you could use Smith fixative (1912) as an alternate: water 230 mL + potassium dichromate 2.5 g + 40% formaldehyde 12.5 mL + acetic acid 6.25 mL This also gives good results. I hope this will help yopu! Ren? J. Ian Montgomery wrote: Student going to Trinidad this summer where she will study frogs. Most of us would simply chill and drink rum but this is a keen young student. Any recommendations for a fixative for tadpoles just as they are about too emerge from the egg. No jelly, just a tadpole inside the egg. Ian. Dr. Ian Montgomery, Histotechnology, IBLS Support Unit, Graham Kerr Building, Tel: 4652, 6644. ian.montgomery@bio.gla.ac.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Blab-away for as little as 1?/min. Make PC-to-Phone Calls using Yahoo! Messenger with Voice. From froyer <@t> bitstream.net Fri May 19 14:38:11 2006 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri May 19 14:38:20 2006 Subject: [Histonet] Tadpoles In-Reply-To: <009701c67b64$576942a0$532ed182@ibls.gla.ac.uk> Message-ID: <000401c67b7b$c3722770$7701a80a@Ford> Ian, I think you have an excellent suggestion embedding in your question... Chilled Rum! I've never tried it on tadpoles, but it has fixed me quite well on occasion. ;-) ~ Ford Ford M. Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL: 612-839-1046 Phone: 763-542-8725 Fax: 763-546-4830 eMail: froyer@bitstream.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian Montgomery Sent: Friday, May 19, 2006 11:51 AM To: Histonet Subject: [Histonet] Tadpoles Student going to Trinidad this summer where she will study frogs. Most of us would simply chill and drink rum but this is a keen young student. Any recommendations for a fixative for tadpoles just as they are about too emerge from the egg. No jelly, just a tadpole inside the egg. Ian. Dr. Ian Montgomery, Histotechnology, IBLS Support Unit, Graham Kerr Building, Tel: 4652, 6644. ian.montgomery@bio.gla.ac.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Malcolm.McCallum <@t> tamut.edu Fri May 19 14:45:49 2006 From: Malcolm.McCallum <@t> tamut.edu (Malcolm McCallum) Date: Fri May 19 14:46:12 2006 Subject: [Histonet] Tadpoles References: <000401c67b7b$c3722770$7701a80a@Ford> Message-ID: alcohol tends to shrink these larvae alot. VISIT THE JOURNAL HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-223-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Ford Royer Sent: Fri 5/19/2006 2:38 PM To: 'Histonet' Subject: RE: [Histonet] Tadpoles Ian, I think you have an excellent suggestion embedding in your question... Chilled Rum! I've never tried it on tadpoles, but it has fixed me quite well on occasion. ;-) ~ Ford Ford M. Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL: 612-839-1046 Phone: 763-542-8725 Fax: 763-546-4830 eMail: froyer@bitstream.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian Montgomery Sent: Friday, May 19, 2006 11:51 AM To: Histonet Subject: [Histonet] Tadpoles Student going to Trinidad this summer where she will study frogs. Most of us would simply chill and drink rum but this is a keen young student. Any recommendations for a fixative for tadpoles just as they are about too emerge from the egg. No jelly, just a tadpole inside the egg. Ian. Dr. Ian Montgomery, Histotechnology, IBLS Support Unit, Graham Kerr Building, Tel: 4652, 6644. ian.montgomery@bio.gla.ac.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ploykasek <@t> phenopath.com Fri May 19 15:21:42 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri May 19 15:21:56 2006 Subject: [Histonet] Fixatives Message-ID: After reading John Kiernan's eloquent dissertation on knowing your fixative, I thought I'd venture on a similar topic. We are a reference lab and receive specimens from all over the USA. One of my "pet peeves" is that it is rare to see in the report exactly what type of fixative the specimen was received in or subsequently processed in. I know we have no standard form of reporting, but it just seems like best practice to me to include this information on the report. One of my favorites is "...received in fixative..." - not very helpful. I could go on & on. At any rate, this is just a bit of a Friday rant. I was wondering how others felt as perhaps I am too far in left field (or maybe just the 'left"coast). Thanks all. Patti Loykasek PhenoPath Laboratories ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From Delventh3 <@t> aol.com Fri May 19 15:59:01 2006 From: Delventh3 <@t> aol.com (Delventh3@aol.com) Date: Fri May 19 15:59:10 2006 Subject: [Histonet] Cryostat disinfecting Message-ID: <462.df1820.319f8b95@aol.com> Thanks to all who wrote to me about disinfecting the cryostat. I received some excellent guidelines. One of the biggest problems was the agent to use for killing AFB. Unfortunately, I miss typed the telephone number, but you all were persistent in getting the info to me. As an aside: After 46 years of working in Histology, I am being forced to quit due to problems with the old ticker. I cannot say how much I have enjoyed this field of work. I was able to combine my teaching certificates and histology certifications in a way that made every day interesting. Now on to other avenues of expression. Quilting, here I come. Good luck to you all. I've enjoyed the banter on Histonet. God Bless! Priscilla Delventhal, BA, HT,. HTL, ASCP From Olivier.Leroux <@t> UGent.be Sat May 20 12:52:08 2006 From: Olivier.Leroux <@t> UGent.be (Olivier Leroux) Date: Sat May 20 12:52:22 2006 Subject: [Histonet] Plant anatomy Message-ID: <1148147528.446f5748c2a8c@mail.ugent.be> Hello, Is there anybody with experience on fluorescent staining? I also wish to communicate with people who work with Technovit 7100 (Plant anatomy). I'm also searching people who would like to help me with the localization of pectins in plant cell walls (TEM, eventually with immunohistochemistry) Best regards, Olivier -- Olivier Leroux http://www.pteridology.ugent.be Olivier.Leroux@UGent.be From katri <@t> cogeco.ca Sat May 20 18:45:06 2006 From: katri <@t> cogeco.ca (Katri Tuomala) Date: Sat May 20 18:45:14 2006 Subject: [Histonet] Fixatives References: Message-ID: <001801c67c67$6c8af6c0$6a9a9618@Katri> I totally agree with Patti. Knowing what the tissue has been fixed in and sometimes how long would be a big help in doing special stains and immunos. It should be standard information given to reference labs. I don't think this issue belongs to Friday rants, it is a valid point! Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "Patti Loykasek" To: "histonet" Sent: Friday, May 19, 2006 4:21 PM Subject: [Histonet] Fixatives > After reading John Kiernan's eloquent dissertation on knowing your > fixative, > I thought I'd venture on a similar topic. We are a reference lab and > receive > specimens from all over the USA. One of my "pet peeves" is that it is rare > to see in the report exactly what type of fixative the specimen was > received > in or subsequently processed in. I know we have no standard form of > reporting, but it just seems like best practice to me to include this > information on the report. One of my favorites is "...received in > fixative..." - not very helpful. I could go on & on. > At any rate, this is just a bit of a Friday rant. I was wondering how > others > felt as perhaps I am too far in left field (or maybe just the > 'left"coast). > Thanks all. > > Patti Loykasek > PhenoPath Laboratories > > > ------------------------------------------------------------------------- > This e-mail message, including any attachments, is for the sole use of > the intended recipients and may contain privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If > you are not the intended recipient, please contact the sender by e-mail > and destroy all copies of the original message, or you may call PhenoPath > Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From barry_m <@t> ozemail.com.au Sun May 21 06:10:34 2006 From: barry_m <@t> ozemail.com.au (Barry Madigan) Date: Sun May 21 06:10:40 2006 Subject: [Histonet] Fixatives In-Reply-To: <001801c67c67$6c8af6c0$6a9a9618@Katri> Message-ID: <000701c67cc7$2f08f490$0201010a@WORKSTATION1> Yes it would be a great help to know how long tissue has been fixed for. Tissue that has been fixed for short times does have a tendency of being damaged by the usual heat or enzyme antigen retrieval protocols, particularly the nuclei detail with the heat. I find this loss of nuclei detail a major problem when dealing with lymphomas. Unfortunately there is no standardisation of fixation not even in our own laboratory let alone the number of laboratories that refer paraffin blocks or slides for Immunohistochemistry. It is a problem that is difficult to control since Immunohistochemistry is usually the last area to deal with cases before the reports are finalised. Also there is the added problem that your control material has not received the same conditions as the test. Thank God for internal controls. Well that was my rant. Barry Madigan Immunohistochemistry QHPS-Central Queensland Australia -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Katri Tuomala Sent: Sunday, 21 May 2006 9:45 AM To: Patti Loykasek; histonet Subject: Re: [Histonet] Fixatives I totally agree with Patti. Knowing what the tissue has been fixed in and sometimes how long would be a big help in doing special stains and immunos. It should be standard information given to reference labs. I don't think this issue belongs to Friday rants, it is a valid point! Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "Patti Loykasek" To: "histonet" Sent: Friday, May 19, 2006 4:21 PM Subject: [Histonet] Fixatives > After reading John Kiernan's eloquent dissertation on knowing your > fixative, > I thought I'd venture on a similar topic. We are a reference lab and > receive > specimens from all over the USA. One of my "pet peeves" is that it is rare > to see in the report exactly what type of fixative the specimen was > received > in or subsequently processed in. I know we have no standard form of > reporting, but it just seems like best practice to me to include this > information on the report. One of my favorites is "...received in > fixative..." - not very helpful. I could go on & on. > At any rate, this is just a bit of a Friday rant. I was wondering how > others > felt as perhaps I am too far in left field (or maybe just the > 'left"coast). > Thanks all. > > Patti Loykasek > PhenoPath Laboratories > > > ------------------------------------------------------------------------- > This e-mail message, including any attachments, is for the sole use of > the intended recipients and may contain privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If > you are not the intended recipient, please contact the sender by e-mail > and destroy all copies of the original message, or you may call PhenoPath > Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.m.vanderloos <@t> amc.uva.nl Mon May 22 02:18:11 2006 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Mon May 22 02:18:22 2006 Subject: [Histonet] Re: rabbit anti-FITC Message-ID: <42a58b42bc32.42bc3242a58b@amc.uva.nl> Hi Liz, Gayle and Andrea, We were quite annoyed here too about discontinuing rabbit anti-FITC by Dako's. After complaining about it Dako let me know that a HRP-labeled rabbit anti-FITC is still available. However, that approach isn't sensitive enough for my application below. I looked for a replacement and found a concentrated rabbit anti-FITC from BioGenesis no. 4510-7804 ([1]www.biogenesis.co.uk). This product still needs to be compared with the original Dako rabbit anti-FITC. When done, I will let you know my findings. The test will be a multistep enzymatic double staining procedure combining an unlabeled mouse primary with a FITC-labeled mouse primary antibody: 1. unlabeled mouse primary 1 2. polymer anti-mouse/HRP 3. blocking step with normal mouse serum 4. FITC-labeled mouse primary 2 5. rabbit anti-FITC 6. goat anti-rabbit/AP 7. visualization of AP activity (Fast Blue BB or Vector Blue) 8. visualization of HRP activity in red (AEC or Vector NovaRed) In this protocol a mouse anti-FITC obviously doesn't fit. Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Thu, 18 May 2006 16:10:05 -0600 From: Gayle Callis Subject: Re: [Histonet] rabbit anti-FITC To: "Elizabeth Chlipala" , Histonet@lists.utsouthwestern.edu This is sad as this antibody worked so nicely. It worked beautifully with Rat antMouse CD4-FITC. Chris van der Loos was the one who clued me in to this antibody, as he used it cleverly for his double staining. Maybe he has a new source for one of equal quality. Gayle Callis At 10:59 AM 5/18/2006, you wrote: >Hello everyone > >I was searching the histonet archives and came across an e-mail from >Gayle Callis about the use of Dako's rabbit anti-FITC antibody for FITC >labeled mouse antibodies on mouse tissue. To make a long story short, >it seems that Dako has discontiued this item, is anyone aware of a good >subsititue for this, the one I was intere! sted in w References 1. http://www.biogenesis.co.uk/ From Yves.Heremans <@t> vub.ac.be Mon May 22 06:33:19 2006 From: Yves.Heremans <@t> vub.ac.be (Yves Heremans) Date: Mon May 22 06:33:29 2006 Subject: [Histonet] CXCR4 antibody/protocol Message-ID: Dear All, Does anyone have information with regard to a good antibody source/protocol for detection of mouse or rat CXCR4 (aka CD184) in paraffin or frozen sections ? Best regards, Yves From syedab <@t> totalise.co.uk Mon May 22 07:20:56 2006 From: syedab <@t> totalise.co.uk (Anila Syed) Date: Mon May 22 07:21:04 2006 Subject: [Histonet] section thickness References: <002b01c67ae3$d35adcb0$6a9a9618@Katri> Message-ID: <01be01c67d9a$2db1cb20$14c401a3@clneuro.ox.ac.uk> Hello, we always get best results with 5um Best wishes Anila Syed > > ----- Original Message ----- > From: "Edwards, R.E." > To: > Sent: Thursday, May 18, 2006 6:43 AM > Subject: [Histonet] section thickness > > > > > > In your invaluable, but, no doubt differing opinions, what is the > optimal section thickness for formalin fixed paraffin processed tissues > for immunostaining, using DAB as chromogen?, there has been some > discussion here whether it should be 4,5,6, or 7microns, many thanks. > > > Richard Edwards > > > MRC TOXICOLOGY UNIT > > LEICESTER..U.K....... > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.392 / Virus Database: 268.6.1/343 - Release Date: 18/05/06 > > From TJJ <@t> Stowers-Institute.org Mon May 22 09:29:16 2006 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Mon May 22 09:29:40 2006 Subject: [Histonet] Re: Bouins alternative/substitute question Message-ID: Jason, You indicate you are ridding the lab of Bouins fixative, and I can only presume it is because of the volitile nature of picric acid. I have not heard of any incidents involving the mixture of saturated picric acid, formaldehyde, and acetic acid. Unless your safety department has disposal issues, I would think it safe to use and store provided you bought it ready-to-use. This is one fixative I have no desire to make from scratch because I do not want the responsibility of storing and monitoring picric acid. We don't use much of it here; only on occasion when a journal article refers to its use, or when another lab has found it is the only agent that allows for detection of certain antigens. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64133 From AFeatherstone <@t> KaleidaHealth.Org Mon May 22 09:35:23 2006 From: AFeatherstone <@t> KaleidaHealth.Org (Featherstone, Annette) Date: Mon May 22 09:35:31 2006 Subject: [Histonet] disinfecting cryostat Message-ID: <9B4A77DF11463E4FB723D484214AE9BCA5523B@KALEXMB02.KaleidaHealth.org> Bleach is a tuberculocide. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, May 19, 2006 11:09 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 30, Issue 26 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. rabbit anti-FITC (Elizabeth Chlipala) 2. Help workers comp issue (Michelle D. Moore) 3. Re: Help workers comp issue (Jackie M O'Connor) 4. Good antibodies for macrophage detection in rat samples (Guillermo Palao) 5. Re: disinfecting the cryostat (Robyn Vazquez) 6. RE: Good antibodies for macrophage detection in rat samples (Elizabeth Chlipala) 7. section thickness for IHC (Osborn, Sharon) 8. specimen size for IHC (Heather Renko) 9. Re: specimen size for IHC (Jackie M O'Connor) 10. Histotech Position in Richmond, VA (Paula Sicurello) 11. rabbit anti-GFP FITC labeled (Elizabeth Chlipala) 12. RE: immunofluorescence amplification... (Andrea T. Hooper) 13. FW: Jb-4 blocks (Allen, Rhonda) 14. Source of Re: [Histonet] IHC - Mycoplasma Ab (Gayle Callis) 15. Re: Sakura Xpress users (Lori Richey) 16. Re: rabbit anti-FITC (Gayle Callis) 17. Re: Good antibodies for macrophage detection in rat samples (Gayle Callis) 18. RE: Sakura Xpress users (Bartlett, Jeanine (CDC/NCID/VR)) 19. Re: rabbit anti-FITC (Andrea T. Hooper) 20. Her 2 question (BSylinda@aol.com) 21. Re: section thickness (Katri Tuomala) 22. Please unsubscibe me from your list server. Thank you. (Barb Nuernberger) 23. Beta-catenin (Jairam Vanamala) 24. Re: Incubation chamber (Geoffrey White) 25. RE: FW: Jb-4 blocks (Edmondson David (RBV) NHS Christie Tr) 26. unsubscribe (Johnson, Cindy, M) 27. (no subject) (ashokc@ncbs.res.in) 28. Histology Position (Patterson, Pat) ---------------------------------------------------------------------- Message: 1 Date: Thu, 18 May 2006 10:59:55 -0600 From: "Elizabeth Chlipala" Subject: [Histonet] rabbit anti-FITC To: Message-ID: <000001c67a9c$7e6a7270$0300a8c0@Chlipala> Content-Type: text/plain; charset="us-ascii" Hello everyone I was searching the histonet archives and came across an e-mail from Gayle Callis about the use of Dako's rabbit anti-FITC antibody for FITC labeled mouse antibodies on mouse tissue. To make a long story short, it seems that Dako has discontiued this item, is anyone aware of a good subsititue for this, the one I was interested in was not biotinylated. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 ------------------------------ Message: 2 Date: Thu, 18 May 2006 11:01:43 -0600 From: "Michelle D. Moore" Subject: [Histonet] Help workers comp issue To: "Histonet" Message-ID: <000601c67a9c$bde339f0$ef00a8c0@tmhpath1> Content-Type: text/plain; charset="iso-8859-1" Hello fellow colleagues, I am at a point of frustration with workers comp, some surprise there huh? I need to be pointed in the direction of getting information about histotechs and repetitive motion disorders. I have a tear on my tendon in my right elbow with epicondylitis and bilateral carpal tunnel syndrome and of course they are saying it is not work related (BULL). I did not have this problem until working in histology and I do not play any sports or anything that would cause these problems. I would really appreciate some help with this as I am going to have to get a lawyer to even get anything done about this. I appreciate all your time and help with this. Sorry to vent but I just got off the phone with workers comp and I am angry and hurt. Thank you again for your time and help. Michelle D. Moore HT(ASCP) The Memorial Hospital 785 Russell St Craig, CO 81625 tmhpath@amigo.net 970-826-3292 Fax 970-826-3158 ------------------------------ Message: 3 Date: Thu, 18 May 2006 12:19:47 -0500 From: "Jackie M O'Connor" Subject: Re: [Histonet] Help workers comp issue To: "Michelle D. Moore" Cc: Histonet Message-ID: Content-Type: text/plain; charset="US-ASCII" Michelle - Speaking as someone who has suffered from tendinitis from microtomy, who says it's not work related? Workmans comp? What is your claim for? Missed work, health care costs, or other damages? What does your doctor say? You'll need supporting documentation from your ortho for any action to be taken seriously. It sounds as if your place of employment isn't cooperating - I slammed my finger in a drawer at work a couple of months ago, and did some nerve damage - I didn't go to employee health until a week later because I thought it was stupid, but then they were all over it - even sent me to a hand specialist. The feeling eventually did come back in my finger (I can tell, you were ALL worried) - anyway - it's basically the doctor's call - and he'll recommend how to prevent additional damage, which probably includes adapting your microtomy style, among other things. Then you probably could get a good personal injury lawyer who will do all the research for 30% of your settlement. Good luck. "Michelle D. Moore" Sent by: histonet-bounces@lists.utsouthwestern.edu 05/18/2006 12:01 PM To "Histonet" cc Subject [Histonet] Help workers comp issue Hello fellow colleagues, I am at a point of frustration with workers comp, some surprise there huh? I need to be pointed in the direction of getting information about histotechs and repetitive motion disorders. I have a tear on my tendon in my right elbow with epicondylitis and bilateral carpal tunnel syndrome and of course they are saying it is not work related (BULL). I did not have this problem until working in histology and I do not play any sports or anything that would cause these problems. I would really appreciate some help with this as I am going to have to get a lawyer to even get anything done about this. I appreciate all your time and help with this. Sorry to vent but I just got off the phone with workers comp and I am angry and hurt. Thank you again for your time and help. Michelle D. Moore HT(ASCP) The Memorial Hospital 785 Russell St Craig, CO 81625 tmhpath@amigo.net 970-826-3292 Fax 970-826-3158 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Thu, 18 May 2006 20:00:41 +0200 (CEST) From: Guillermo Palao Subject: [Histonet] Good antibodies for macrophage detection in rat samples To: Histonet Message-ID: <20060518180041.17105.qmail@web26206.mail.ukl.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello all, We are interested in detecting macrophages in FFPE samples from rat. I would really appreciate any suggestions regarding the best antigen (Is F4-80 the best?) and most useful commercial antibody to use. Thanks in advance, Guillermo --------------------------------- LLama Gratis a cualquier PC del Mundo. Llamadas a fijos y m?viles desde 1 c?ntimo por minuto. http://es.voice.yahoo.com ------------------------------ Message: 5 Date: Thu, 18 May 2006 11:00:46 -0700 From: "Robyn Vazquez" Subject: Re: [Histonet] disinfecting the cryostat To: delventh3@aol.com, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii We use a product that is a broad-spectrum, SaniMaster IV Google it. Shut cryo down, drench inside with diluted SaniMaster IV, let set for awhile, drain, wipe out, spray with 100%, wipe out, air dry for awhile, plug back in, wah la...tried calling number, but got a not in service announcement. Hope this helps Robyn OHSU >>> 5/18/2006 8:56 AM >>> Does anyone have a protocol for disinfecting the cryostat? Specifically a substance that is specific for AFB. If you could give a call to anyone of us at the Histology Lab we would appreciate it. Thanks in advance. Priscilla Histology, St. Anthony's Hospital, Rockford, Ill. 815-395-3410 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Thu, 18 May 2006 12:09:17 -0600 From: "Elizabeth Chlipala" Subject: RE: [Histonet] Good antibodies for macrophage detection in rat samples To: "'Guillermo Palao'" , "'Histonet'" Message-ID: <000701c67aa6$2df0e680$0300a8c0@Chlipala> Content-Type: text/plain; charset="iso-8859-1" F4/80 is a murine macrophage marker. I use ED-1 from serotec, it's a mouse anti-rat macrophage marker. It works well on FFPE tissue with proteinase K digestion, (even works on formic acid decalcifed bone). Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Guillermo Palao Sent: Thursday, May 18, 2006 12:01 PM To: Histonet Subject: [Histonet] Good antibodies for macrophage detection in rat samples Hello all, We are interested in detecting macrophages in FFPE samples from rat. I would really appreciate any suggestions regarding the best antigen (Is F4-80 the best?) and most useful commercial antibody to use. Thanks in advance, Guillermo --------------------------------- LLama Gratis a cualquier PC del Mundo. Llamadas a fijos y m?viles desde 1 c?ntimo por minuto. http://es.voice.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Thu, 18 May 2006 14:17:13 -0400 From: "Osborn, Sharon" Subject: [Histonet] section thickness for IHC To: Message-ID: <9A919A5D70313A4D9C56A025710874080C72B2@kenmsg40.us.schp.com> Content-Type: text/plain; charset="us-ascii" We do ours at 5 microns, brains, CNS are at 10-15 microns sharon osborn DNAX, SP BioPharma Palo Alto, CA Subject: [Histonet] section thickness In your invaluable, but, no doubt differing opinions, what is the optimal section thickness for formalin fixed paraffin processed tissues for immunostaining, using DAB as chromogen?, there has been some discussion here whether it should be 4,5,6, or 7microns, many thanks. Richard Edwards MRC TOXICOLOGY UNIT LEICESTER..U.K. ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. ------------------------------ Message: 8 Date: Thu, 18 May 2006 11:45:14 -0700 (PDT) From: Heather Renko Subject: [Histonet] specimen size for IHC To: histonet@pathology.swmed.edu Message-ID: <20060518184514.12226.qmail@web31305.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 The optimum size for IHC is 3-4mm thick and 1-2cm square to allow adequate penetration in the fixation process. The Immunochemical Staining handbook(3rd Ed, published by Dako) states to cut at 4-6. Heather Renko In your invaluable, but, no doubt differing opinions, what is the optimal section thickness for formalin fixed paraffin processed tissues for immunostaining, using DAB as chromogen?, there has been some discussion here whether it should be 4,5,6, or 7microns, many thanks. Richard Edwards --------------------------------- Ring'em or ping'em. Make PC-to-phone calls as low as 1?/min with Yahoo! Messenger with Voice. ------------------------------ Message: 9 Date: Thu, 18 May 2006 14:34:55 -0500 From: "Jackie M O'Connor" Subject: Re: [Histonet] specimen size for IHC To: Heather Renko Cc: histonet@pathology.swmed.edu Message-ID: Content-Type: text/plain; charset="UTF-8" CM Van der Loos is the "Dr. House of Histology". I believe whatever he says unequivocally. I pulled this from the Jan 06 archives. [Histonet] RE: optimal thickness for cutting of IHC sections From: "C.M. van der Loos" Dear Paul, I totally agree what you wrote about IHC: it's just a surface event. Long time ago we had to prove that our (prehistoric) attempt of quantifying an IHC technique wasn't influenced by tissue thickness due to microtome abberations. We did cut (cryostat) sections of different thickness and guess what: there was hardly any influence on the staining intensity. The only thing we observed was that thicker sections showed a higher non-specific background staining than thinner sections. See: CM van der Lo os, MMH Marijianowski and AE Becker: Quantification in immunohistochemistry. The measurement of the ratio?EUR(tm)s between collagen types I and I II. Histochem J (1994) 26, 347-354<= /P> Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M 2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Nether lands Date: Tue, 3 Jan 2006 13:09:20 -0500 From: "Monfils, Paul" Subject : RE: [Histonet] optimal thickness for cutting of IHC sections< BR>To: 'histonet@lists.utsouthwestern.edu' I believe section thickness is less critical for IHC because antibodies are very large molecules that don't penetrate tissue very well, so regardless of the thickness of the section, you are really only staining the exposed surface of the tissue, perhaps to a depth of 2 microns or so. We have verified this by electron microscopy. This is quite different from standard histochemical procedures where a thicker section results in a more intense stain because the small dye molecules penetrate the tissue readily and stain it all the way through. Heather Renko Sent by: histonet-bounces@lists.utsouthwestern.edu 05/18/2006 01:45 PM To histonet@pathology.swmed.edu cc Subject [Histonet] specimen size for IHC The optimum size for IHC is 3-4mm thick and 1-2cm square to allow adequate penetration in the fixation process. The Immunochemical Staining handbook(3rd Ed, published by Dako) states to cut at 4-6. Heather Renko In your invaluable, but, no doubt differing opinions, what is the optimal section thickness for formalin fixed paraffin processed tissues for immunostaining, using DAB as chromogen?, there has been some discussion here whether it should be 4,5,6, or 7microns, many thanks. Richard Edwards --------------------------------- Ring'em or ping'em. Make PC-to-phone calls as low as 1??/min with Yahoo! Messenger with Voice. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Thu, 18 May 2006 16:16:24 -0400 From: "Paula Sicurello" Subject: [Histonet] Histotech Position in Richmond, VA To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello Fellow Histologists, The Medical College of Virgini histotechnologists. We have 2 hi The posting only shows the one position as yet, but there will be 2 jobs. Please go to [1]www.vcuhealth.org and look under the Allied Health section of the job listings. It lists the hours but I don't know how set those are. If you're interested, you can e-mail me a copy of your resume and I'll pass it along to the powers that be. Also, please fill out the o Thanks to all who are interested, Paula :-) References 1. 3D"http://www.vcuhealth.org"=/ ------------------------------ Message: 11 Date: Thu, 18 May 2006 14:23:47 -0600 From: "Elizabeth Chlipala" Subject: [Histonet] rabbit anti-GFP FITC labeled To: "'Histonet'" Message-ID: <000601c67ab8$f7d4d710$0300a8c0@Chlipala> Content-Type: text/plain; charset="us-ascii" Hello Does anyone out there know of a good rabbit anti-GFP that FITC labeled. The client I'm working with wants an affinity purified antibody if possible. Thanks in advance. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 ------------------------------ Message: 12 Date: Thu, 18 May 2006 16:59:15 -0400 From: "Andrea T. Hooper" Subject: [Histonet] RE: immunofluorescence amplification... To: histonet@pathology.swmed.edu Message-ID: Content-Type: text/plain; charset=us-ascii; format=flowed I find using a biotinylated secondary and Streptavidin labeled with fluorophore also works well for amplication, certainly a step up from directly labeled secondaries where amplification is desired. Good luck! Andrea At 8:56 AM +0200 5/17/06, C.M. van der Loos wrote: > > > Date: Mon, 15 May 2006 16:44:28 -0700 (PDT) > From: Adam Perry > Subject: [Histonet] immunofluorescence amplification... > To: histonet@pathology.swmed.edu > What are people's impressions about different (i.e. the best) ways to > amplify fluorescent signal from immunocytochemistry? > I'm working with 30 um rodent brain sections. When I use a > biotinylated secondary antibody with avidin-biotin-HRP detection with > DAB or NiDAB I get good cellular staining with a primary antibody > concentration of 1:1,000. > I am now trying to perform double labeling and so have switched to > fluorescence...and I don't seem to be getting any specific signal > now. > I have tried increasing the antibody concentration from 1:1,000 up to > 1:100 and still don't see the faintest hint of staining (and the > background just gets really bad). > I've used the fluorescent antibody to detect other primaries (so the > secondary is working in general and my mi! croscope >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- ------------------------------ Message: 13 Date: Thu, 18 May 2006 16:45:27 -0500 From: "Allen, Rhonda" Subject: [Histonet] FW: Jb-4 blocks To: Message-ID: Content-Type: text/plain; charset="us-ascii" > Histonetter's, > > The following question was posted on the microscopy list serve by a > friend. I thought maybe some of the folks in histoland might have > some good suggestions. > > Rhonda Allen > > > "We are doing a rush job for a client who requires 4.0 um sections > from JB 4 blocks. Our ultramicrotomists extraordinaire, Cheryl, is > having a dickens of a time getting the sections to remain flat when > removing them from the knife. She is cutting on glass and taking > sections from the dry edge with a fine forceps. As soon as the > sections leave the knife, they curl and won't uncurl when placed on a > drop of water on a slide. > > Not only is this a rush job in support of a grant proposal, but it > requires serial sectioning with no missing sections, and we have, > like, > no real experience with this resin. Cheryl has tried various sized > block faces and different thicknesses for the sections, but nothing is > helping. > > The thumping sound you hear is a head hitting a wall----repeatedly. > Can anyone HEEEELLLLP??" > > ------------------------------ Message: 14 Date: Thu, 18 May 2006 16:00:52 -0600 From: Gayle Callis Subject: Source of Re: [Histonet] IHC - Mycoplasma Ab To: "Jan Shivers" , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20060518155847.01b31680@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Richard Cartun gave out a fabulous source, ViroStat out of Portland Maine. Just go to ViroStat website and shop - Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) ------------------------------ Message: 15 Date: Thu, 18 May 2006 15:01:10 -0700 From: Lori Richey Subject: Re: [Histonet] Sakura Xpress users To: Angela Bitting Cc: histonet@lists.utsouthwestern.edu Message-ID: <446CEEA6.3090006@u.washington.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed We use the xpress processor for most biopsy specimens like liver, cervical, prostate, and oral path. Our pathologists have not agreed to put the renal, skin biopsies or the breast biopsies on the express. We also still run all of the regular surgical specimens on the VIP. These work well on the xpress, however, they must be cut in at the recommended size limits. Also, due to the fixation time in formalin for most of the large specimens it works better to load them in the VIP in formalin for a couple hours before the processor runs. There are only 2 end chambers on the Xpress, meaning someone has to be present to unload it. Most of the special stains and the IHC staining is comparable to routine processing. The Trich stain on liver biopsies done on the Artisan special stainer has been the most challenging. Angela Bitting wrote: >I'd love to get opinions from hospital Histology labs using the Xpress processor. Our Director went off to "War College" and heard all about it and you all know what happens next............. What are the benefits and drawbacks to it? > >Angela Bitting, HT(ASCP) >Technical Specialist, Histology >Geisinger Medical Center >100 N Academy Ave. MC 23-00 >Danville, PA 17822 >phone 570-214-9634 >fax 570-271-5916 > > > >IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 16 Date: Thu, 18 May 2006 16:10:05 -0600 From: Gayle Callis Subject: Re: [Histonet] rabbit anti-FITC To: "Elizabeth Chlipala" , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20060518160705.01b39f00@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed This is sad as this antibody worked so nicely. It worked beautifully with Rat antMouse CD4-FITC. Chris van der Loos was the one who clued me in to this antibody, as he used it cleverly for his double staining. Maybe he has a new source for one of equal quality. Gayle Callis At 10:59 AM 5/18/2006, you wrote: >Hello everyone > >I was searching the histonet archives and came across an e-mail from >Gayle Callis about the use of Dako's rabbit anti-FITC antibody for FITC >labeled mouse antibodies on mouse tissue. To make a long story short, >it seems that Dako has discontiued this item, is anyone aware of a good >subsititue for this, the one I was interested in was not biotinylated. > >Thanks in advance > >Liz > >Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC >Manager >Premier Laboratory, LLC >P.O. Box 18592 >Boulder, Colorado 80308 >Office: (303) 735-5001 >Fax: (303) 735-3540 >liz@premierlab.com >www.premierlab.com > >Ship to Address: >Premier Laboratory >University of Colorado >MCDB, Room A3B40 >Boulder, Colorado 80309 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Thu, 18 May 2006 16:15:42 -0600 From: Gayle Callis Subject: Re: [Histonet] Good antibodies for macrophage detection in rat samples To: Guillermo Palao , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20060518161117.01b49008@gemini.msu.montana.edu> Content-Type: text/plain; charset="iso-8859-1"; format=flowed F4-80 ( a rat antimouse monoclonal) is for mouse macrophages, not rat. There are several well documented rat macrophage antibodies, go to Serotec and look for these. ED1, ED2, ED3 are subsets. These mouse antiRat monoclonals were detected with goat antimouse secondaries that mouse IgG isotypes (IgG1, etc) biotinylated from Southern Biotechnology. At 12:00 PM 5/18/2006, you wrote: >Hello all, > > We are interested in detecting macrophages in FFPE samples from rat. I > would really appreciate any suggestions regarding the best antigen (Is > F4-80 the best?) and most useful commercial antibody to use. > > Thanks in advance, > > Guillermo > > >--------------------------------- > >LLama Gratis a cualquier PC del Mundo. >Llamadas a fijos y m?viles desde 1 c?ntimo por minuto. >http://es.voice.yahoo.com >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) ------------------------------ Message: 18 Date: Thu, 18 May 2006 18:29:05 -0400 From: "Bartlett, Jeanine \(CDC/NCID/VR\)" Subject: RE: [Histonet] Sakura Xpress users To: "Lori Richey" , "Angela Bitting" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="UTF-8" Lori: What is the conflict with the pathologists regarding the renal, skin and breast biopsies being run on the Xpress? Thanks. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Lori Richey Sent: Thu 5/18/2006 6:01 PM To: Angela Bitting Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Sakura Xpress users We use the xpress processor for most biopsy specimens like liver, cervical, prostate, and oral path. Our pathologists have not agreed to put the renal, skin biopsies or the breast biopsies on the express. We also still run all of the regular surgical specimens on the VIP. These work well on the xpress, however, they must be cut in at the recommended size limits. Also, due to the fixation time in formalin for most of the large specimens it works better to load them in the VIP in formalin for a couple hours before the processor runs. There are only 2 end chambers on the Xpress, meaning someone has to be present to unload it. Most of the special stains and the IHC staining is comparable to routine processing. The Trich stain on liver biopsies done on the Artisan special stainer has been the most challenging. Angela Bitting wrote: >I'd love to get opinions from hospital Histology labs using the Xpress processor. Our Director went off to "War College" and heard all about it and you all know what happens next............. What are the benefits and drawbacks to it? > >Angela Bitting, HT(ASCP) >Technical Specialist, Histology >Geisinger Medical Center >100 N Academy Ave. MC 23-00 >Danville, PA 17822 >phone 570-214-9634 >fax 570-271-5916 > > > >IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Thu, 18 May 2006 18:36:17 -0400 From: "Andrea T. Hooper" Subject: Re: [Histonet] rabbit anti-FITC To: Histonet , liz@premierlab.com Message-ID: Content-Type: text/plain; charset=us-ascii; format=flowed Bummer, I loved this antibody also for mouse on mouse staining. DAKO sells a mouse anti-FITC, clone DAK-FITC4, which is excellent for staining human tissue with FITC labeled antibodies using the Mouse EnVision+ kit as the detection system. I am hoping this antibody was not discontinued as well! Also FYI, there is a sheep anti-FITC-HRP (from the TUNEL POD kit but sold separately also) from Roche Molecular Biochemicals which is excellent and I routintely use it for conversion of good strong labeling FITC antibodies to HRP/DAB detection. (The primary must obviously stain robustly since there is a lack of amplification using this protocol). With just a quick scan of InVitrogen/Zymed/Molecular Probes website .... I see a rabbit anti-FITC from Zymed Catalog #71-1900, I know nothing about its quality though, probably decent! -- Andrea >At 10:59 AM 5/18/2006, you wrote: >>Hello everyone >> >>I was searching the histonet archives and came across an e-mail from >>Gayle Callis about the use of Dako's rabbit anti-FITC antibody for FITC >>labeled mouse antibodies on mouse tissue. To make a long story short, >>it seems that Dako has discontiued this item, is anyone aware of a good >>subsititue for this, the one I was interested in was not biotinylated. >> >>Thanks in advance >> >>Liz >> >>Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC >>Manager >>Premier Laboratory, LLC >>P.O. Box 18592 >>Boulder, Colorado 80308 >>Office: (303) 735-5001 >>Fax: (303) 735-3540 >>liz@premierlab.com >>www.premierlab.com -- ------------------------------ Message: 20 Date: Thu, 18 May 2006 19:27:37 EDT From: BSylinda@aol.com Subject: [Histonet] Her 2 question To: histonet@lists.utsouthwestern.edu Message-ID: <3e5.2d9bf9d.319e5ce9@aol.com> Content-Type: text/plain; charset="US-ASCII" Hello Histoland, Can anyone out there can give me some info about if you are using a multi control for Her 2. We currently send out Her 2, and we are getting a single 1 punch control equal to a 3 plus control. My question is how do you run a 3 plus control in house and without running a multi control which has 0, 1+, and 3+. All feedback will be greatly appreciated, Thanks in advance Sylinda Battle HT ASCP ------------------------------ Message: 21 Date: Thu, 18 May 2006 21:30:34 -0400 From: "Katri Tuomala" Subject: Re: [Histonet] section thickness To: "Edwards, R.E." , Message-ID: <002b01c67ae3$d35adcb0$6a9a9618@Katri> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Richard, I do all my immunos at 3 um, always have for the last 20 years... Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "Edwards, R.E." To: Sent: Thursday, May 18, 2006 6:43 AM Subject: [Histonet] section thickness In your invaluable, but, no doubt differing opinions, what is the optimal section thickness for formalin fixed paraffin processed tissues for immunostaining, using DAB as chromogen?, there has been some discussion here whether it should be 4,5,6, or 7microns, many thanks. Richard Edwards MRC TOXICOLOGY UNIT LEICESTER..U.K....... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 22 Date: Thu, 18 May 2006 21:45:24 -0400 From: "Barb Nuernberger" Subject: [Histonet] Please unsubscibe me from your list server. Thank you. To: Message-ID: Content-Type: text/plain; charset=US-ASCII Please unsubscibe me from your list server. Thank you. Barb Nuernberger ------------------------------ Message: 23 Date: Thu, 18 May 2006 19:23:39 -0700 (PDT) From: Jairam Vanamala Subject: [Histonet] Beta-catenin To: histonet@lists.utsouthwestern.edu Message-ID: <20060519022339.56541.qmail@web31806.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Does anyone has the working IHC protocol for beta-catenin, preferably for rat colon. Jairam Vanamala Post-doc TAMU College Station, TX __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com ------------------------------ Message: 24 Date: Fri, 19 May 2006 05:52:10 -0700 (PDT) From: Geoffrey White Subject: Re: [Histonet] Incubation chamber To: Histonet@lists.utsouthwestern.edu Cc: jatindra_prakash@yahoo.com Message-ID: <20060519125210.64906.qmail@web38107.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Try out QInstruments. There you will find a new incubation chamber for a little price. Good Luck Geoffrey __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com ------------------------------ Message: 25 Date: Fri, 19 May 2006 14:35:44 +0100 From: "Edmondson David \(RBV\) NHS Christie Tr" Subject: RE: [Histonet] FW: Jb-4 blocks To: "Allen, Rhonda" , Message-ID: <3C83687E8F6AE04792E361ABE2D385B8418115@cht-mail2-2k.xchristie.nhs.uk> Content-Type: text/plain; charset="us-ascii" Hi, It's years ago, but I remember something along the lines of 70% alc, and picking up the section with a brush in a droplet of the alcohol and flicking it onto a staining pot of water. They span and the creases came out . Then picked up onto slides and dried on a hotplate. Does that approach sound familiar to anyone? David Christie Hosp Manchester UK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Allen, Rhonda Sent: 18 May 2006 22:45 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Jb-4 blocks > Histonetter's, > > The following question was posted on the microscopy list serve by a > friend. I thought maybe some of the folks in histoland might have > some good suggestions. > > Rhonda Allen > > > "We are doing a rush job for a client who requires 4.0 um sections > from JB 4 blocks. Our ultramicrotomists extraordinaire, Cheryl, is > having a dickens of a time getting the sections to remain flat when > removing them from the knife. She is cutting on glass and taking > sections from the dry edge with a fine forceps. As soon as the > sections leave the knife, they curl and won't uncurl when placed on a > drop of water on a slide. > > Not only is this a rush job in support of a grant proposal, but it > requires serial sectioning with no missing sections, and we have, > like, > no real experience with this resin. Cheryl has tried various sized > block faces and different thicknesses for the sections, but nothing is > helping. > > The thumping sound you hear is a head hitting a wall----repeatedly. > Can anyone HEEEELLLLP??" > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************** This e-mail and any files transmitted with it are confidential and solely for the use of the intended recipient. If you have received this e-mail in error you should not disseminate, distribute or copy it. Please notify the sender immediately and delete this e-mail from your system. ******************************************************************************************************************* ------------------------------ Message: 26 Date: Fri, 19 May 2006 08:57:32 -0500 From: "Johnson, Cindy, M" Subject: [Histonet] unsubscribe To: Message-ID: Content-Type: text/plain; charset="us-ascii" Please unsubscribe me from your list server. Thank you. ------------------------------ Message: 27 Date: Fri, 19 May 2006 19:46:09 +0530 (IST) From: ashokc@ncbs.res.in Subject: [Histonet] (no subject) To: Histonet@lists.utsouthwestern.edu Message-ID: <1249.59.92.206.50.1148048169.squirrel@59.92.206.50> Content-Type: text/plain;charset=iso-8859-1 please unsubscribe from histonet Ashok.C Research Scholar Prof.M.M.Panicker's lab Molecular Neurobiology National Centre for Biological Sciences GKVK Campus Bangalore-560065 Phone-+91-080-23636421 ext :2251 Fax no-91-080-23636662 ------------------------------ Message: 28 Date: Fri, 19 May 2006 09:52:59 -0500 From: "Patterson, Pat" Subject: [Histonet] Histology Position To: Message-ID: <293C7C19EFF7D611AE1A0002A53F8114164AC225@omega.mhd.com> Content-Type: text/plain; charset="us-ascii" Methodist Dallas Medical Center - voted "One of the Best Places to Work in Dallas" currently has a full-time 2nd shift position available for a certified Histology Technician/Technologist. Experience in all aspects of Histology are required. Frozen section and Immunofluoresence experience is desired. We also have a PRN position available for either 1st or 2nd shift hours. Contact : Pat Patterson 214-947-3538 patpatterson@mhd.com OR Lorena Davila, HR recruiter lorenadavila@mhd.com *********************************************************************** This electronic transmission contains information from Methodist Health System and should be considered confidential and privileged. The information contained in the above messages is intended only for the use of the individual(s) and entity(ies) named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution, or use of this information is prohibited. If you receive this transmission in error, please notify the sender immediately by return e-mail. Methodist Health System, its subsidiaries and affiliates hereby claim all applicable privileges related to the transmission of this communication. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 30, Issue 26 **************************************** CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From vazquezr <@t> ohsu.edu Mon May 22 09:55:46 2006 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Mon May 22 09:56:18 2006 Subject: [Histonet] disinfecting cryostat Message-ID: Bleach is also a corrosive. From settembr <@t> umdnj.edu Mon May 22 10:24:45 2006 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Mon May 22 10:25:38 2006 Subject: [Histonet] Hairy cell leukaemia- CD103 Message-ID: I have hairy cell leukaemia, clone DBA44 that I have worked up for formlin fixed paraffin embedded (FFPE) human tissue. My director wants CD103 for hairy cell leukaemia. I know that there are vendors who cell CD103 for OTHER applications. Has anyone gotten CD103 to work on FFPE human tissue? paraffin. Thank you Dana Settembre University Hospital-UMDNJ Newark, NJ From kemlo.rogerson <@t> waht.swest.nhs.uk Mon May 22 10:27:27 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon May 22 10:27:22 2006 Subject: [Histonet] Re: Bouins alternative/substitute question Message-ID: I think it is because picric acid explodes when dry but I've tried, and tried, and tried and it has never exploded. Has anyone ever succeeded? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Education is not the filling of a pail, but the lighting of a fire. --W. B. Yeats This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From TJJ <@t> Stowers-Institute.org Mon May 22 10:33:24 2006 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Mon May 22 10:33:57 2006 Subject: [Histonet] Re: Bouins alternative/substitute question Message-ID: Kemlo, exactly. But my point is that it is in a solution with other chemicals, and I presume that adds a measure of stability. ~tj -----Original Message----- From: Kemlo Rogerson [mailto:kemlo.rogerson@waht.swest.nhs.uk] Sent: Monday, May 22, 2006 10:27 AM To: Johnson, Teri; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Bouins alternative/substitute question I think it is because picric acid explodes when dry but I've tried, and tried, and tried and it has never exploded. Has anyone ever succeeded? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Education is not the filling of a pail, but the lighting of a fire. --W. B. Yeats This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Jackie.O'Connor <@t> abbott.com Mon May 22 10:53:58 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Mon May 22 10:54:38 2006 Subject: [Histonet] Re: Bouins alternative/substitute question In-Reply-To: Message-ID: Kemlo - If no one responds - it's because they were blown to smithereens. I have a video of a bottle of picric acid that blew up in a training demo - pretty nasty. Jackie O' "Johnson, Teri" Sent by: histonet-bounces@lists.utsouthwestern.edu 05/22/2006 10:33 AM To "Kemlo Rogerson" , cc Subject RE: [Histonet] Re: Bouins alternative/substitute question Kemlo, exactly. But my point is that it is in a solution with other chemicals, and I presume that adds a measure of stability. ~tj -----Original Message----- From: Kemlo Rogerson [mailto:kemlo.rogerson@waht.swest.nhs.uk] Sent: Monday, May 22, 2006 10:27 AM To: Johnson, Teri; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Bouins alternative/substitute question I think it is because picric acid explodes when dry but I've tried, and tried, and tried and it has never exploded. Has anyone ever succeeded? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Education is not the filling of a pail, but the lighting of a fire. --W. B. Yeats This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Mon May 22 10:56:57 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Mon May 22 10:57:28 2006 Subject: [Histonet] Re: Bouins alternative/substitute question In-Reply-To: Message-ID: The explosion described here was the largest man made explosion on record until the nuclear age. http://en.wikipedia.org/wiki/Halifax_Explosion From bhewlett <@t> cogeco.ca Mon May 22 11:04:14 2006 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Mon May 22 11:04:21 2006 Subject: [Histonet] Re: Bouins alternative/substitute question References: Message-ID: <001c01c67db9$5f90ba60$6500a8c0@mainbox> Yes! Bryan ----- Original Message ----- From: "Kemlo Rogerson" To: "'Johnson, Teri'" ; Sent: Monday, May 22, 2006 11:27 AM Subject: RE: [Histonet] Re: Bouins alternative/substitute question >I think it is because picric acid explodes when dry but I've tried, and > tried, and tried and it has never exploded. > > Has anyone ever succeeded? > > Kemlo Rogerson > Pathology Manager > Ext 3311 > DD 01934 647057 > Mob 07749 754194 > > Education is not the filling of a pail, but the lighting of a fire. --W. > B. > Yeats > > > > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on > its > contents: to do so is strictly prohibited and may be unlawful. Please > inform > me that this message has gone astray before deleting it. Thank you for > your > co-operation > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sjchtascp <@t> yahoo.com Mon May 22 11:13:43 2006 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Mon May 22 11:13:46 2006 Subject: [Histonet] seeking position Message-ID: <20060522161343.62535.qmail@web38205.mail.mud.yahoo.com> I'm seeking a clinical or research HT position in south central WI or north central Illinos. Full, part, or on-call. Excellent references, 14+ years experiance. Steven Coakley HT(ASCP) __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From cgfields <@t> lexhealth.org Mon May 22 11:20:58 2006 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Mon May 22 11:17:00 2006 Subject: FW: [Histonet] Re: Bouins alternative/substitute question Message-ID: -----Original Message----- From: Carole Fields Sent: Monday, May 22, 2006 11:44 AM To: 'Kemlo Rogerson' Subject: RE: [Histonet] Re: Bouins alternative/substitute question Two institutions I know of (friends working there)..... called the bomb squad to pick it up and they exploded it...they said it would take out a city block. That is what they were told......I would not try and explode it. I had it hauled from a lab where I worked in Calif. And they were careful to have someone dispose of it also. Carole Fields, HT,ASCP Pathology Supervisor Lexington Medical Center 2720 Sunset Blvd. W. Columbia, SC 29169 -----Original Message----- From: Kemlo Rogerson [mailto:kemlo.rogerson@waht.swest.nhs.uk] Sent: Monday, May 22, 2006 11:27 AM To: 'Johnson, Teri'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Bouins alternative/substitute question I think it is because picric acid explodes when dry but I've tried, and tried, and tried and it has never exploded. Has anyone ever succeeded? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Education is not the filling of a pail, but the lighting of a fire. --W. B. Yeats This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. From POWELL_SA <@t> Mercer.edu Mon May 22 11:25:24 2006 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Mon May 22 11:26:00 2006 Subject: [Histonet] Re: Bouins alternative/substitute question In-Reply-To: Message-ID: <01M2Q0RTTF7W8WW9YS@Macon2.Mercer.edu> Yes Kemlo and all, that happened here in "humid" Georgia where you may think it not possible for the chemical to dry out. The Bomb squad made a crater large enough in which to park 2 buses at the police rifle range detonating a small bottle of the stuff. I personally would STOP trying to make it explode. :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carole Fields Sent: Monday, May 22, 2006 11:21 AM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] Re: Bouins alternative/substitute question -----Original Message----- From: Carole Fields Sent: Monday, May 22, 2006 11:44 AM To: 'Kemlo Rogerson' Subject: RE: [Histonet] Re: Bouins alternative/substitute question Two institutions I know of (friends working there)..... called the bomb squad to pick it up and they exploded it...they said it would take out a city block. That is what they were told......I would not try and explode it. I had it hauled from a lab where I worked in Calif. And they were careful to have someone dispose of it also. Carole Fields, HT,ASCP Pathology Supervisor Lexington Medical Center 2720 Sunset Blvd. W. Columbia, SC 29169 -----Original Message----- From: Kemlo Rogerson [mailto:kemlo.rogerson@waht.swest.nhs.uk] Sent: Monday, May 22, 2006 11:27 AM To: 'Johnson, Teri'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Bouins alternative/substitute question I think it is because picric acid explodes when dry but I've tried, and tried, and tried and it has never exploded. Has anyone ever succeeded? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Education is not the filling of a pail, but the lighting of a fire. --W. B. Yeats This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Mon May 22 11:34:18 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon May 22 11:34:23 2006 Subject: [Histonet] Re: Bouins alternative/substitute question In-Reply-To: References: Message-ID: <6.0.0.22.1.20060522102519.01b38358@gemini.msu.montana.edu> Kemlo, Interesting that you said this, and also a story from our university chemical safety handling of dry picric acid found in an old laboratory. They had a hard time getting it to explode also. Chemical safety removed a jar of picric acid, dry and age undetermined. Knowing it was explosive, the fellow in charge took it out to a very remote area and shot at the jar with a rifle, and it took a lot of rifle bullets to get it to blow up. He did have a good session of target practice for deer hunting season being in the Wild Wild West of Montana. The potential is there for explosion, and we still have picric acid on our shelves, although stored under a generous layer of distilled water. When we need to make up Bouins, we just scoop it out and make sure we have a saturated solution of picric acid. Care must be taken to keep dry crystals off lids, these are actually sealed by dipping into hot paraffin, and check regularly for any leakage around lid. I don't think it would be a good thing to have in a laboratory fire, but solvents are just as bad. How many people still store isopentane in a non explosion proof freezer - now that IS an explosive situation. We remain cautious but not in panic state. Bouins is still very important as a fixative and mordant for Massons trichrome, we will continue to use it, dispose of it correctly and not put metal cassettes into it. We often purchase it ready made from Sigma to avoid having to handle it. At 09:27 AM 5/22/2006, you wrote: >I think it is because picric acid explodes when dry but I've tried, and >tried, and tried and it has never exploded. > >Has anyone ever succeeded? > >Kemlo Rogerson >Pathology Manager >Ext 3311 >DD 01934 647057 >Mob 07749 754194 > >Education is not the filling of a pail, but the lighting of a fire. --W. B. >Yeats > > > > >This e-mail is confidential and privileged. If you are not the intended >recipient please accept my apologies; please do not disclose, copy or >distribute information in this e-mail or take any action in reliance on its >contents: to do so is strictly prohibited and may be unlawful. Please inform >me that this message has gone astray before deleting it. Thank you for your >co-operation > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From cgfields <@t> lexhealth.org Mon May 22 11:55:10 2006 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Mon May 22 11:51:18 2006 Subject: [Histonet] Re: Bouins alternative/substitute question Message-ID: This is totally off the subject but.... I was reading an article in the May 06 Smithsonian Magazine at my doctors office and noticed Gayle Callis's name in the article about dinosaurs. It was a wonderful article about some really exciting new discoveries in Montana. Everyone should read this wonderful article. Congratulations on being involved in this exciting project. Carole Fields Lexington, SC -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: Monday, May 22, 2006 12:34 PM To: Kemlo Rogerson; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Bouins alternative/substitute question Kemlo, Interesting that you said this, and also a story from our university chemical safety handling of dry picric acid found in an old laboratory. They had a hard time getting it to explode also. Chemical safety removed a jar of picric acid, dry and age undetermined. Knowing it was explosive, the fellow in charge took it out to a very remote area and shot at the jar with a rifle, and it took a lot of rifle bullets to get it to blow up. He did have a good session of target practice for deer hunting season being in the Wild Wild West of Montana. The potential is there for explosion, and we still have picric acid on our shelves, although stored under a generous layer of distilled water. When we need to make up Bouins, we just scoop it out and make sure we have a saturated solution of picric acid. Care must be taken to keep dry crystals off lids, these are actually sealed by dipping into hot paraffin, and check regularly for any leakage around lid. I don't think it would be a good thing to have in a laboratory fire, but solvents are just as bad. How many people still store isopentane in a non explosion proof freezer - now that IS an explosive situation. We remain cautious but not in panic state. Bouins is still very important as a fixative and mordant for Massons trichrome, we will continue to use it, dispose of it correctly and not put metal cassettes into it. We often purchase it ready made from Sigma to avoid having to handle it. At 09:27 AM 5/22/2006, you wrote: >I think it is because picric acid explodes when dry but I've tried, and >tried, and tried and it has never exploded. > >Has anyone ever succeeded? > >Kemlo Rogerson >Pathology Manager >Ext 3311 >DD 01934 647057 >Mob 07749 754194 > >Education is not the filling of a pail, but the lighting of a fire. --W. B. >Yeats > > > > >This e-mail is confidential and privileged. If you are not the intended >recipient please accept my apologies; please do not disclose, copy or >distribute information in this e-mail or take any action in reliance on its >contents: to do so is strictly prohibited and may be unlawful. Please inform >me that this message has gone astray before deleting it. Thank you for your >co-operation > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. From Janet.Bonner <@t> FLHOSP.ORG Mon May 22 11:59:16 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Mon May 22 12:01:18 2006 Subject: [Histonet] Re: Bouins alternative/substitute question References: Message-ID: In what context was her name used?! She can't be that old! ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Carole Fields Sent: Mon 5/22/2006 12:55 PM To: 'Gayle Callis' Cc: HistoNet (E-mail) Subject: RE: [Histonet] Re: Bouins alternative/substitute question This is totally off the subject but.... I was reading an article in the May 06 Smithsonian Magazine at my doctors office and noticed Gayle Callis's name in the article about dinosaurs. It was a wonderful article about some really exciting new discoveries in Montana. Everyone should read this wonderful article. Congratulations on being involved in this exciting project. Carole Fields Lexington, SC -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: Monday, May 22, 2006 12:34 PM To: Kemlo Rogerson; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Bouins alternative/substitute question Kemlo, Interesting that you said this, and also a story from our university chemical safety handling of dry picric acid found in an old laboratory. They had a hard time getting it to explode also. Chemical safety removed a jar of picric acid, dry and age undetermined. Knowing it was explosive, the fellow in charge took it out to a very remote area and shot at the jar with a rifle, and it took a lot of rifle bullets to get it to blow up. He did have a good session of target practice for deer hunting season being in the Wild Wild West of Montana. The potential is there for explosion, and we still have picric acid on our shelves, although stored under a generous layer of distilled water. When we need to make up Bouins, we just scoop it out and make sure we have a saturated solution of picric acid. Care must be taken to keep dry crystals off lids, these are actually sealed by dipping into hot paraffin, and check regularly for any leakage around lid. I don't think it would be a good thing to have in a laboratory fire, but solvents are just as bad. How many people still store isopentane in a non explosion proof freezer - now that IS an explosive situation. We remain cautious but not in panic state. Bouins is still very important as a fixative and mordant for Massons trichrome, we will continue to use it, dispose of it correctly and not put metal cassettes into it. We often purchase it ready made from Sigma to avoid having to handle it. At 09:27 AM 5/22/2006, you wrote: >I think it is because picric acid explodes when dry but I've tried, and >tried, and tried and it has never exploded. > >Has anyone ever succeeded? > >Kemlo Rogerson >Pathology Manager >Ext 3311 >DD 01934 647057 >Mob 07749 754194 > >Education is not the filling of a pail, but the lighting of a fire. --W. B. >Yeats > > > > >This e-mail is confidential and privileged. If you are not the intended >recipient please accept my apologies; please do not disclose, copy or >distribute information in this e-mail or take any action in reliance on its >contents: to do so is strictly prohibited and may be unlawful. Please inform >me that this message has gone astray before deleting it. Thank you for your >co-operation > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sandosis <@t> uia.net Mon May 22 12:22:14 2006 From: sandosis <@t> uia.net (sandosis@uia.net) Date: Mon May 22 12:22:23 2006 Subject: [Histonet] Supervisor Job In Orange County CA Message-ID: <200605221722.k4MHMFFq040124@smtp3.uia.net> Histology Supervisor Position in Orange County California Great opportunity for IHC experienced histologist. Must have IHC, general hospital histology skills and supervisory experience. Please e-mail resumes to the Clinical Laboratory Operations Manager, val.grinenko@stjoe.org --------------------------------------------- This message was sent using the UIA Web Mail Server. ULTIMATE Internet Access, Inc http://www.uia.net/ From johanna.jackson <@t> csc.mrc.ac.uk Mon May 22 12:26:40 2006 From: johanna.jackson <@t> csc.mrc.ac.uk (Jackson, Johanna) Date: Mon May 22 12:27:40 2006 Subject: [Histonet] tyrosine hydroxylase staining?? Message-ID: <2A1F3E27491CBF46BC14ACB3E5C7D89AB55B74@icex3.ic.ac.uk> Dear All, I am trying to stain dopaminergic neurons in the striatum of the adult rat brain which has been perfused with 4% PFA, washed and then cryoprotected in 20% sucrose, before being sectioned (10um) on a cryostat. I pretreat the sections with 0.1% Triton-X and block using serum. I am using the monoclonal anti-tyrosine hydroxylase antibody from Sigma (T1299). Sigma recommend a working concentration of 1:2000 however I have had no luck with this staining. I just a weak staining all over the brain with no specificity in the striatum. Does anyone have a protocol using this particular antibody? I have seen protocols with anti-TH from other companies which follow a similar method to what I have described above, however they do not seem to work for the Sigma antibody. Any help would be greatly appreciated! Thanks! Jo Stem Cell Imaging MRC Clinical Sciences Centre Imperial College London Hammersmith Hospital Campus Du Cane Road London W12 0NN 0208 3833796 From TJJ <@t> Stowers-Institute.org Mon May 22 12:30:24 2006 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Mon May 22 12:30:52 2006 Subject: [Histonet] Re: Smithsonian article Message-ID: Hmmm...at the risk of totally offending my workshop partner... Montana, dinosaurs, and Gayle Callis, all mentioned in the same article... Sorry Gayle, you know I love ya! Just couldn't resist that temptation... Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64133 From gcallis <@t> montana.edu Mon May 22 12:34:48 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon May 22 12:35:09 2006 Subject: [Histonet] Off the subject magazine article In-Reply-To: References: Message-ID: <6.0.0.22.1.20060522112355.01b57da8@gemini.msu.montana.edu> The article was fun to read, but my part in the project, as reported or written up was totally inaccurate - the person writing this up did not contact me about my involvement in the project, probably something they never do. I did some work teaching the PhD student about MMA plastic embedding, but I never would have taken slides to some meeting to entice pathologists to look at it nor am I a molecular biologist!!! The project was totally proprietary at the time i.e. you don't give out people research projects without their permission. But I did teach the dinosaur people on our campus that one could embed extinct bone (T. rex, very dry, filled with sand, dirt, etc) in poly methylmethacrylate plastic, cut with diamond saws, and grind thin sections. This was done years and years ago, back in Jurassic Park ages. So be careful that you read this kind of publication with tongue in cheek - the facts are not always correct - at least I am in the Smithsonian archives now and in a few years they can put my old bones there too - propped up next to T. Rex. And thanks for the congratulations - At 10:55 AM 5/22/2006, you wrote: >This is totally off the subject but.... I was reading an article in the >May 06 Smithsonian Magazine at my doctors office and noticed Gayle >Callis's name in the article about dinosaurs. It was a wonderful article >about some really exciting new discoveries in Montana. Everyone should >read this wonderful article. Congratulations on being involved in this >exciting project. > >Carole Fields >Lexington, SC > >-----Original Message----- >From: Gayle Callis [mailto:gcallis@montana.edu] >Sent: Monday, May 22, 2006 12:34 PM >To: Kemlo Rogerson; Histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] Re: Bouins alternative/substitute question > >Kemlo, > >Interesting that you said this, and also a story from our university >chemical safety handling of dry picric acid found in an old >laboratory. They had a hard time getting it to explode also. > >Chemical safety removed a jar of picric acid, dry and age >undetermined. Knowing it was explosive, the fellow in charge took it out >to a very remote area and shot at the jar with a rifle, and it took a lot >of rifle bullets to get it to blow up. He did have a good session of >target practice for deer hunting season being in the Wild Wild West of >Montana. > >The potential is there for explosion, and we still have picric acid on our >shelves, although stored under a generous layer of distilled water. When >we need to make up Bouins, we just scoop it out and make sure we have a >saturated solution of picric acid. > >Care must be taken to keep dry crystals off lids, these are actually sealed >by dipping into hot paraffin, and check regularly for any leakage around >lid. I don't think it would be a good thing to have in a laboratory fire, >but solvents are just as bad. How many people still store isopentane in a >non explosion proof freezer - now that IS an explosive situation. > > We remain cautious but not in panic state. Bouins is still very >important as a fixative and mordant for Massons trichrome, we will continue >to use it, dispose of it correctly and not put metal cassettes into it. We >often purchase it ready made from Sigma to avoid having to handle it. > >At 09:27 AM 5/22/2006, you wrote: > >I think it is because picric acid explodes when dry but I've tried, and > >tried, and tried and it has never exploded. > > > >Has anyone ever succeeded? > > > >Kemlo Rogerson > >Pathology Manager > >Ext 3311 > >DD 01934 647057 > >Mob 07749 754194 > > > >Education is not the filling of a pail, but the lighting of a fire. --W. B. > >Yeats > > > > > > > > > >This e-mail is confidential and privileged. If you are not the intended > >recipient please accept my apologies; please do not disclose, copy or > >distribute information in this e-mail or take any action in reliance on its > >contents: to do so is strictly prohibited and may be unlawful. Please > inform > >me that this message has gone astray before deleting it. Thank you for your > >co-operation > > > > > > > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.u > tsouthwestern.edu/mailman/listinfo/histonet > >Gayle Callis >MT,HT,HTL(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University - Bozeman >PO Box 173610 >Bozeman MT 59717-3610 >406 994-6367 >406 994-4303 (FAX) > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_________________________________ >This email and any files transmitted with it may contain PRIVILEGED or >CONFIDENTIAL information and may be read or used only by the intended >recipient. If you are not the intended recipient of the email or any of >its attachments, please be advised that you have received this email in >error and that any use, dissemination, distribution, forwarding, printing, >or copying of this email or any attached files is strictly prohibited. If >you have received this email in error, please immediately purge it and all >attachments and notify the sender by reply email or contact the sender at >the number listed above if one is provided. Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 From histo20 <@t> hotmail.com Mon May 22 12:35:17 2006 From: histo20 <@t> hotmail.com (Paula Wilder) Date: Mon May 22 12:35:25 2006 Subject: [Histonet] (no subject) Message-ID: Hi! I was just wondering how everyone handles a lost block situation. Do you have policies or procedures? Do you write an incident report? What do you do? Thanks for all your help! Paula Wilder St. Joseph Medical Cente Towson, MD 21204 From gcallis <@t> montana.edu Mon May 22 12:50:32 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon May 22 12:50:37 2006 Subject: [Histonet] Re: Smithsonian article In-Reply-To: References: Message-ID: <6.0.0.22.1.20060522113927.01b65d60@gemini.msu.montana.edu> Dear friends, Oh dear, I am rolling on the floor with laughter!!!! An otherwise mundane Monday has just become a brighter day!!! Callisaurous!!! Well, I am old as dirt - so might as well put me in with the dinosaurs - Gayle Callis ----------- still laughing -------------------------- At 11:30 AM 5/22/2006, you wrote: >Hmmm...at the risk of totally offending my workshop partner... > >Montana, dinosaurs, and Gayle Callis, all mentioned in the same >article... > >Sorry Gayle, you know I love ya! Just couldn't resist that temptation... > >Teri Johnson, HT(ASCP)QIHC >Managing Director Histology Facility >Stowers Institute for Medical Research >1000 E. 50th St. >Kansas City, MO 64133 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ryaskovich <@t> dir.nidcr.nih.gov Mon May 22 12:51:29 2006 From: ryaskovich <@t> dir.nidcr.nih.gov (Yaskovich, Ruth A (NIH/NIDCR) [E]) Date: Mon May 22 12:51:33 2006 Subject: [Histonet] tyrosine hydroxylase staining?? References: <2A1F3E27491CBF46BC14ACB3E5C7D89AB55B74@icex3.ic.ac.uk> Message-ID: Johanna, When I stain rat brain for Tyrosine Hydroxylase I'm staining Paraffin embedding brain that has been perfused with 4% PFA. I use a 1:20 dilution on Midbrain and am getting great results. I'm using the Dako envision products and I'm not sure where I order the antibody from but will find out for you. Ruth Yaskovich National Institutes of Health N.I.D.C.R. Pain and Neurosensory Mechanisms Branch ________________________________ From: Jackson, Johanna [mailto:johanna.jackson@csc.mrc.ac.uk] Sent: Mon 5/22/2006 1:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] tyrosine hydroxylase staining?? Dear All, I am trying to stain dopaminergic neurons in the striatum of the adult rat brain which has been perfused with 4% PFA, washed and then cryoprotected in 20% sucrose, before being sectioned (10um) on a cryostat. I pretreat the sections with 0.1% Triton-X and block using serum. I am using the monoclonal anti-tyrosine hydroxylase antibody from Sigma (T1299). Sigma recommend a working concentration of 1:2000 however I have had no luck with this staining. I just a weak staining all over the brain with no specificity in the striatum. Does anyone have a protocol using this particular antibody? I have seen protocols with anti-TH from other companies which follow a similar method to what I have described above, however they do not seem to work for the Sigma antibody. Any help would be greatly appreciated! Thanks! Jo Stem Cell Imaging MRC Clinical Sciences Centre Imperial College London Hammersmith Hospital Campus Du Cane Road London W12 0NN 0208 3833796 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sjchtascp <@t> yahoo.com Mon May 22 13:13:08 2006 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Mon May 22 13:13:14 2006 Subject: [Histonet] looking for a position Message-ID: <20060522181308.23365.qmail@web38212.mail.mud.yahoo.com> More specificly in the Rockford Illinois, north to Madison, WI Steve __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From cfavara <@t> niaid.nih.gov Mon May 22 13:41:45 2006 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID) [E]) Date: Mon May 22 13:41:52 2006 Subject: [Histonet] PCNA on mouse Message-ID: Anyone done any PCNA on mouse FFPE? I have a few references but would rather have the real word. Thanks, c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives From brent.jeter <@t> gmail.com Mon May 22 13:55:35 2006 From: brent.jeter <@t> gmail.com (brent jeter) Date: Mon May 22 13:55:45 2006 Subject: [Histonet] Workload Message-ID: <1d93a6240605221155n60409d4by6c2fabf5729d23e0@mail.gmail.com> Some feedback, please. I'm just stepping into an AP manager position and have a workload question (my background is as a Cytotech): are there any guidelines or recommendations for workload levels for Histotechs? Thanks. Brent Jeter From Jenny-Oblander <@t> omrf.ouhsc.edu Mon May 22 13:56:30 2006 From: Jenny-Oblander <@t> omrf.ouhsc.edu (Jenny Oblander) Date: Mon May 22 13:56:39 2006 Subject: [Histonet] PCNA on mouse Message-ID: Zymed has a wonderful kit out for PCNA on mouse FFPE tissue. Very clean and relatively easy. Jenny -----Original Message----- From: Favara, Cynthia (NIH/NIAID) [E] [mailto:cfavara@niaid.nih.gov] Sent: Monday, May 22, 2006 1:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PCNA on mouse Anyone done any PCNA on mouse FFPE? I have a few references but would rather have the real word. Thanks, c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon May 22 14:04:28 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon May 22 14:04:33 2006 Subject: [Histonet] Workload In-Reply-To: <1d93a6240605221155n60409d4by6c2fabf5729d23e0@mail.gmail.com> Message-ID: <20060522190428.710.qmail@web61223.mail.yahoo.com> Brent: Under separate cover I am sending you an article I just finished on the subject. Ren? J. brent jeter wrote: Some feedback, please. I'm just stepping into an AP manager position and have a workload question (my background is as a Cytotech): are there any guidelines or recommendations for workload levels for Histotechs? Thanks. Brent Jeter _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. From Janet.Bonner <@t> FLHOSP.ORG Mon May 22 14:03:29 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Mon May 22 14:04:44 2006 Subject: [Histonet] (no subject) References: Message-ID: We save our trash until all of the slides are given out and the cases are confirmed complete. If there is a block missing we turn the lab up-side-down and see what shakes out. We've found blocks left in surface decal, in the microtome chucks, in the trash (pushed off the counter by mistake), filed without sectioning, and even UNDER the microtome! If you still can't find it, an incident report is order. This doesn't happen often but errors occur. -Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Paula Wilder Sent: Mon 5/22/2006 1:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi! I was just wondering how everyone handles a lost block situation. Do you have policies or procedures? Do you write an incident report? What do you do? Thanks for all your help! Paula Wilder St. Joseph Medical Cente Towson, MD 21204 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Mon May 22 14:07:22 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Mon May 22 14:07:31 2006 Subject: [Histonet] (no subject) Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202736B37@sjhaexc02.sjha.org> and don't forget lab coat pockets and hems! We've even found them there.. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bonner, Janet Sent: Monday, May 22, 2006 3:03 PM To: Paula Wilder; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (no subject) We save our trash until all of the slides are given out and the cases are confirmed complete. If there is a block missing we turn the lab up-side-down and see what shakes out. We've found blocks left in surface decal, in the microtome chucks, in the trash (pushed off the counter by mistake), filed without sectioning, and even UNDER the microtome! If you still can't find it, an incident report is order. This doesn't happen often but errors occur. -Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Paula Wilder Sent: Mon 5/22/2006 1:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi! I was just wondering how everyone handles a lost block situation. Do you have policies or procedures? Do you write an incident report? What do you do? Thanks for all your help! Paula Wilder St. Joseph Medical Cente Towson, MD 21204 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From LGaliotto <@t> nch.org Mon May 22 14:35:53 2006 From: LGaliotto <@t> nch.org (Galiotto, Laura) Date: Mon May 22 14:36:02 2006 Subject: [Histonet] Part-time Histotech opening Message-ID: <270614B321ACB44D8C1D91F4F921FDC3036CBD63@NCH01EX02.nch.org> I have two positions open, part-time M-F, and hourly on call ( to work every Saturday and if available as needed). Must be HT (ASCP). Interested parties can call me or complete an application on line at NCH.ORG Laura Galiotto, HT (ASCP) Histology Facilitator Northwest Community Hospital 800 West Central Road Arlington Heights, Il 60005 847-618-6190 ******************** PLEASE NOTE ******************** This E-mail/telefax message and any documents accompanying this transmission may contain information that is previleged, confidential, and/or exempt from disclosure under applicable law and is intended soley for the addressee(s) named above. If you are not the intended addressee/recipient, you are hereby notified that any use of, disclosure, copying, distribution, or reliance on the contents of this E-Mail/telefax information is strictly prohibited and may result in legal action against you. Please reply to the sender advising of the error in transmission and immediately delete/destroy the message and any accompanying documents. Thank you. From JGREWE <@t> OhioHealth.com Mon May 22 15:00:55 2006 From: JGREWE <@t> OhioHealth.com (JGREWE@OhioHealth.com) Date: Mon May 22 15:01:01 2006 Subject: [Histonet] Jacquelyn Grewe/Staff/OhioHealth is out of the office . Message-ID: I will be out of the office starting 05/22/2006 and will not return until 06/05/2006. I will respond to your message when I return. Thanks, Jackie From stephanie.brusig <@t> weyerhaeuser.com Mon May 22 16:08:53 2006 From: stephanie.brusig <@t> weyerhaeuser.com (Brusig, Stephanie) Date: Mon May 22 16:09:01 2006 Subject: [Histonet] RE: plant anatomy Message-ID: <16E971922EDF9A4B9E8D3216374EDD49012B7BD8@wafedixm12.corp.weyer.pri> I use Technovit 7100 right now to section fir and pine embryos. I will be starting fluorescence, but will embed in paraffin instead of plastic. What is your question regarding Technovit or fluorescence? Not sure if I can help, but I can try. ~Stephanie Brusig Weyerhaeuser Company Propagation of High Value Trees 32901 Weyerhaeuser Way S WTC-1B10 Federal Way, WA 98001 253.924.6518 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Sunday, May 21, 2006 10:09 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 30, Issue 29 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Plant anatomy (Olivier Leroux) 2. Re: Fixatives (Katri Tuomala) 3. RE: Fixatives (Barry Madigan) ---------------------------------------------------------------------- Message: 1 Date: Sat, 20 May 2006 19:52:08 +0200 From: Olivier Leroux Subject: [Histonet] Plant anatomy To: histonet@lists.utsouthwestern.edu Message-ID: <1148147528.446f5748c2a8c@mail.ugent.be> Content-Type: text/plain; charset=ISO-8859-1 Hello, Is there anybody with experience on fluorescent staining? I also wish to communicate with people who work with Technovit 7100 (Plant anatomy). I'm also searching people who would like to help me with the localization of pectins in plant cell walls (TEM, eventually with immunohistochemistry) Best regards, Olivier -- Olivier Leroux http://www.pteridology.ugent.be Olivier.Leroux@UGent.be ------------------------------ Message: 2 Date: Sat, 20 May 2006 19:45:06 -0400 From: "Katri Tuomala" Subject: Re: [Histonet] Fixatives To: "Patti Loykasek" , "histonet" Message-ID: <001801c67c67$6c8af6c0$6a9a9618@Katri> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original I totally agree with Patti. Knowing what the tissue has been fixed in and sometimes how long would be a big help in doing special stains and immunos. It should be standard information given to reference labs. I don't think this issue belongs to Friday rants, it is a valid point! Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "Patti Loykasek" To: "histonet" Sent: Friday, May 19, 2006 4:21 PM Subject: [Histonet] Fixatives > After reading John Kiernan's eloquent dissertation on knowing your > fixative, > I thought I'd venture on a similar topic. We are a reference lab and > receive > specimens from all over the USA. One of my "pet peeves" is that it is rare > to see in the report exactly what type of fixative the specimen was > received > in or subsequently processed in. I know we have no standard form of > reporting, but it just seems like best practice to me to include this > information on the report. One of my favorites is "...received in > fixative..." - not very helpful. I could go on & on. > At any rate, this is just a bit of a Friday rant. I was wondering how > others > felt as perhaps I am too far in left field (or maybe just the > 'left"coast). > Thanks all. > > Patti Loykasek > PhenoPath Laboratories > > > ------------------------------------------------------------------------ - > This e-mail message, including any attachments, is for the sole use of > the intended recipients and may contain privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If > you are not the intended recipient, please contact the sender by e-mail > and destroy all copies of the original message, or you may call PhenoPath > Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Sun, 21 May 2006 21:10:34 +1000 From: "Barry Madigan" Subject: RE: [Histonet] Fixatives To: "'Katri Tuomala'" , Message-ID: <000701c67cc7$2f08f490$0201010a@WORKSTATION1> Content-Type: text/plain; charset="us-ascii" Yes it would be a great help to know how long tissue has been fixed for. Tissue that has been fixed for short times does have a tendency of being damaged by the usual heat or enzyme antigen retrieval protocols, particularly the nuclei detail with the heat. I find this loss of nuclei detail a major problem when dealing with lymphomas. Unfortunately there is no standardisation of fixation not even in our own laboratory let alone the number of laboratories that refer paraffin blocks or slides for Immunohistochemistry. It is a problem that is difficult to control since Immunohistochemistry is usually the last area to deal with cases before the reports are finalised. Also there is the added problem that your control material has not received the same conditions as the test. Thank God for internal controls. Well that was my rant. Barry Madigan Immunohistochemistry QHPS-Central Queensland Australia -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Katri Tuomala Sent: Sunday, 21 May 2006 9:45 AM To: Patti Loykasek; histonet Subject: Re: [Histonet] Fixatives I totally agree with Patti. Knowing what the tissue has been fixed in and sometimes how long would be a big help in doing special stains and immunos. It should be standard information given to reference labs. I don't think this issue belongs to Friday rants, it is a valid point! Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "Patti Loykasek" To: "histonet" Sent: Friday, May 19, 2006 4:21 PM Subject: [Histonet] Fixatives > After reading John Kiernan's eloquent dissertation on knowing your > fixative, > I thought I'd venture on a similar topic. We are a reference lab and > receive > specimens from all over the USA. One of my "pet peeves" is that it is rare > to see in the report exactly what type of fixative the specimen was > received > in or subsequently processed in. I know we have no standard form of > reporting, but it just seems like best practice to me to include this > information on the report. One of my favorites is "...received in > fixative..." - not very helpful. I could go on & on. > At any rate, this is just a bit of a Friday rant. I was wondering how > others > felt as perhaps I am too far in left field (or maybe just the > 'left"coast). > Thanks all. > > Patti Loykasek > PhenoPath Laboratories > > > ------------------------------------------------------------------------ - > This e-mail message, including any attachments, is for the sole use of > the intended recipients and may contain privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If > you are not the intended recipient, please contact the sender by e-mail > and destroy all copies of the original message, or you may call PhenoPath > Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 30, Issue 29 **************************************** From nienhuis <@t> ucla.edu Mon May 22 16:25:56 2006 From: nienhuis <@t> ucla.edu (nienhuis@ucla.edu) Date: Mon May 22 16:26:09 2006 Subject: [Histonet] Re: Bouins alternative/substitute question In-Reply-To: References: Message-ID: <20060522142556.gvmozwqlw808g4oc@mail.ucla.edu> The article is available online if anyone wants to see it. Bob Nienhuis nienhuis@ucla.edu Quoting Carole Fields : > This is totally off the subject but.... I was reading an article in the May > 06 Smithsonian Magazine at my doctors office and noticed Gayle Callis's name > in the article about dinosaurs. It was a wonderful article about some > really exciting new discoveries in Montana. Everyone should read this > wonderful article. Congratulations on being involved in this exciting > project. > Carole Fields > Lexington, SC > > > -----Original Message----- > From: Gayle Callis [mailto:gcallis@montana.edu] > Sent: Monday, May 22, 2006 12:34 PM > To: Kemlo Rogerson; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Bouins alternative/substitute question > > > Kemlo, > > Interesting that you said this, and also a story from our university > chemical safety handling of dry picric acid found in an old > laboratory. They had a hard time getting it to explode also. > > Chemical safety removed a jar of picric acid, dry and age > undetermined. Knowing it was explosive, the fellow in charge took it out > to a very remote area and shot at the jar with a rifle, and it took a lot > of rifle bullets to get it to blow up. He did have a good session of > target practice for deer hunting season being in the Wild Wild West of > Montana. > > The potential is there for explosion, and we still have picric acid on our > shelves, although stored under a generous layer of distilled water. When > we need to make up Bouins, we just scoop it out and make sure we have a > saturated solution of picric acid. > > Care must be taken to keep dry crystals off lids, these are actually sealed > by dipping into hot paraffin, and check regularly for any leakage around > lid. I don't think it would be a good thing to have in a laboratory fire, > but solvents are just as bad. How many people still store isopentane in a > non explosion proof freezer - now that IS an explosive situation. > > We remain cautious but not in panic state. Bouins is still very > important as a fixative and mordant for Massons trichrome, we will continue > to use it, dispose of it correctly and not put metal cassettes into it. We > often purchase it ready made from Sigma to avoid having to handle it. > > At 09:27 AM 5/22/2006, you wrote: >> I think it is because picric acid explodes when dry but I've tried, and >> tried, and tried and it has never exploded. >> >> Has anyone ever succeeded? >> >> Kemlo Rogerson >> Pathology Manager >> Ext 3311 >> DD 01934 647057 >> Mob 07749 754194 >> >> Education is not the filling of a pail, but the lighting of a fire. --W. B. >> Yeats >> >> >> >> >> This e-mail is confidential and privileged. If you are not the intended >> recipient please accept my apologies; please do not disclose, copy or >> distribute information in this e-mail or take any action in reliance on its >> contents: to do so is strictly prohibited and may be unlawful. Please > inform >> me that this message has gone astray before deleting it. Thank you for your >> co-operation >> >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 > 406 994-4303 (FAX) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _________________________________ > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the email or any of its > attachments, please be advised that you have received this email in error > and that any use, dissemination, distribution, forwarding, printing, or > copying of this email or any attached files is strictly prohibited. If you > have received this email in error, please immediately purge it and all > attachments and notify the sender by reply email or contact the sender at > the number listed above if one is provided. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From RSRICHMOND <@t> aol.com Mon May 22 16:43:53 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Mon May 22 16:44:00 2006 Subject: [Histonet] Re: Bouins alternative/substitute question Message-ID: <467.128e496.31a38a99@aol.com> Picric acid is an explosion hazard when dry, particularly if it is contaminated with metal picrates (such as can be formed from a metal cap on the jar). It's shipped with 10% water, and is not explosive unless it dries out. You can prepare Bouin's fixative without ever handling solid picric acid by purchasing saturated picric acid in water, used for the Jaffe reaction for creatinine so that it's easy to get (it's in the current Sigma catalog, for example). But if you use Bouin's fixative at all, you still have to dispose of it, and that can be expensive. Look at the Dapsons' book Hazardous Materials in the Histopathology Laboratory, 4th ed. 2005, for more information. Bob Richmond Gastonia NC From Shirley_PHUA <@t> hsa.gov.sg Mon May 22 21:31:30 2006 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Mon May 22 21:31:48 2006 Subject: [Histonet] DNA/RNA Extraction Kits for paraffinised tissues Message-ID: Dear All Have anyone heard of any DNA/RNA extraction kits for formalin-fixed, paraffinised tissues? I will greatly appreciate if you can share some of your feedbacks. Were your yields good? Thanks in advance! Shirley Phua Histopathology Laboratory Centre for Forensic Medicine Health Sciences Authority DID : 62130654 From judy.stephens <@t> syngenta.com Tue May 23 01:50:44 2006 From: judy.stephens <@t> syngenta.com (judy.stephens@syngenta.com) Date: Tue May 23 01:51:03 2006 Subject: [Histonet] fixative Message-ID: We are having a problem with vaculation in our brain sections. The problem is variable in an embed so I do not think that it is a processing problem. I think perhaps it maybe that the concentration of formalin in the fix which we buy in could be variable. Has anyone had a problem with' too much water' in the fix causing vaculation.if so did they have a method to calculate the amount of formaldehyde present in the fix.? Judy Stephens From kemlo.rogerson <@t> waht.swest.nhs.uk Tue May 23 01:56:45 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue May 23 01:56:42 2006 Subject: [Histonet] fixative Message-ID: Poor fixation by any means will cause nuclear 'bubbling' or allow it to be caused by subsequent heat. If the fault is poor bought in fixative, change Supplier. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Education is not the filling of a pail, but the lighting of a fire. --W. B. Yeats This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From kemlo.rogerson <@t> waht.swest.nhs.uk Tue May 23 01:58:24 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue May 23 01:58:22 2006 Subject: [Histonet] Re: Bouins alternative/substitute question Message-ID: OK, I'll try again See nothi.................................................. Late Kemlo Rogerson EX Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Education is not the filling of a pail, but the lighting of a fire. --W. B. Yeats This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From djamesnz <@t> orcon.net.nz Tue May 23 01:58:14 2006 From: djamesnz <@t> orcon.net.nz (Darren James) Date: Tue May 23 01:58:34 2006 Subject: [Histonet] Re: Bouins alternative/substitute question In-Reply-To: Message-ID: <001301c67e36$44668120$0301010a@DAZZA> The 227 tonnes TNT on board may have had something to do with that too :-) Guess they learnt the hard way about the need for dangerous goods cabinets. Shame their OHS rep wasn't on board ;-) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Tuesday, 23 May 2006 3:57 a.m. To: histonet@lists.utsouthwestern.edu; Kemlo Rogerson Subject: RE: [Histonet] Re: Bouins alternative/substitute question The explosion described here was the largest man made explosion on record until the nuclear age. http://en.wikipedia.org/wiki/Halifax_Explosion _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gmondragon <@t> gsopath.com Tue May 23 02:21:59 2006 From: gmondragon <@t> gsopath.com (Gus Mondragon) Date: Tue May 23 02:22:16 2006 Subject: [Histonet] Uranyl Nitrate on Warthi Starry procedures Message-ID: <03B63DE44B5C014D84F9A9D8A968B5174C4D79@lithium.corp.gsopath.com> Can someone tell me what to use in place of Uranyl Nitrate in the Warthin Starry procedures. Using this chemical is very unsafe as well very expensive to dispose of. Is there antibodies against the organism we can use in IHC. Gus Mondragon (gmondragon@gsopath.com From gillian.2.brown <@t> gsk.com Tue May 23 02:53:59 2006 From: gillian.2.brown <@t> gsk.com (gillian.2.brown@gsk.com) Date: Tue May 23 02:53:44 2006 Subject: [Histonet] DNA/RNA Extraction Kits for paraffinised tissues In-Reply-To: Message-ID: Dear Shirley, there is a short technical report called 'Direct quantification of gene expression in homogenates of formalin-fixed, paraffin-embedded tissues' by a group from Genospectra, Inc, Fremont, CA, USA (guess the reagents are their own) in BioTechniques April 2006 Vol 40 No4 pg481 I think you can get it on line from www.biotechniques.com. It may be a starting point to answer some of your questions. Regards Gill Brown Target Localisation Target Discovery ri- CEDD. GlaxoSmithKline Medicines Research Centre, Gunnelswood Road, STEVENAGE, Hertfordshire. "Shirley PHUA" Sent by: histonet-bounces@lists.utsouthwestern.edu 23-May-2006 03:31 To histonet@lists.utsouthwestern.edu cc Subject [Histonet] DNA/RNA Extraction Kits for paraffinised tissues Dear All Have anyone heard of any DNA/RNA extraction kits for formalin-fixed, paraffinised tissues? I will greatly appreciate if you can share some of your feedbacks. Were your yields good? Thanks in advance! Shirley Phua Histopathology Laboratory Centre for Forensic Medicine Health Sciences Authority DID : 62130654 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Tue May 23 03:20:38 2006 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Tue May 23 03:21:32 2006 Subject: [Histonet] re PCNA Message-ID: <003801c67e41$c5f01f20$112b5c9f@Carlos> Check out the picture gallery here for some of my pwax pics of PCNA/Ki67 and H3 ......... http://immunoportal.com/index.php I would strongly suggest using Ki67 as a more accurate marker as PCNA has such a long half life. Also, Histone 3 (H3) will label mitotic cells. Carl From RSRICHMOND <@t> aol.com Tue May 23 03:22:42 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Tue May 23 03:22:48 2006 Subject: [Histonet] Re: Fixatives Message-ID: <41a.1ecaee5.31a42052@aol.com> Patti Loykasek at PhenoPath Laboratories notes >>We are a reference lab and receive specimens from all over the USA. One of my "pet peeves" is that it is rare to see in the report exactly what type of fixative the specimen was received in or subsequently processed in. I know we have no standard form of reporting, but it just seems like best practice to me to include this information on the report. One of my favorites is "...received in fixative..." - not very helpful.<< That's exactly the phrase I use in my gross descriptions, and for a very good reason. I'm not about to stick my nose into every specimen bottle to verify that it contains formalin and not alcohol, water, or pine-scented floor disinfectant (used at one hospital I know as a "fixative" for placentas). I'm willing to smell-test a very occasional container where I'm suspicious that the wrong fixative has been used, but not every time! Time of fixation is the dead horse in the middle of the living room floor - nobody wants to hear that the HER2 immunostain for breast cancer requires overnight fixation, for example. Bob Richmond Gastonia NC From louise.renton <@t> gmail.com Tue May 23 03:35:54 2006 From: louise.renton <@t> gmail.com (louise renton) Date: Tue May 23 03:35:58 2006 Subject: [Histonet] DNA/RNA Extraction Kits for paraffinised tissues In-Reply-To: References: Message-ID: Dear Shirley, My information may be somewhat out of date as it is several years since I did DNA extration on formalin fixed /praffin embedded tissues, but at that time I found that the commercial kits (specifically the ones using spin columns) were not as good as the old fashioned method of phenol chloroform. The amount/quality of DNA available is directly related to the type and length of fixation so harsh unbuffered fixation will degrade the DNA. This is my 2 cents worth On 5/23/06, Shirley PHUA wrote: > Dear All > > Have anyone heard of any DNA/RNA extraction kits for formalin-fixed, > paraffinised tissues? I will greatly appreciate if you can share some of > your feedbacks. Were your yields good? > > Thanks in advance! > > Shirley Phua > Histopathology Laboratory > Centre for Forensic Medicine > Health Sciences Authority > DID : 62130654 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin From carl.hobbs <@t> kcl.ac.uk Tue May 23 03:43:07 2006 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Tue May 23 03:43:30 2006 Subject: [Histonet] Re: tyrosine hydroxylase staining?? Message-ID: <003e01c67e44$e9e235f0$112b5c9f@Carlos> I do not have a protocol for that antibody. I use what was BMannheim's( now sold by Chemicon : mab 5280) anti TH on mouse pwax sections. Please visit here for a pic, if you wish. http://immunoportal.com/index.php However, I haven't used it on frozen sections. Hope it' s helpful From Janet.Bonner <@t> FLHOSP.ORG Tue May 23 06:20:16 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Tue May 23 06:21:16 2006 Subject: [Histonet] Re: Bouins alternative/substitute question References: Message-ID: Kemlo..? ...Kemlo...? ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kemlo Rogerson Sent: Tue 5/23/2006 2:58 AM To: 'Carole Fields'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Bouins alternative/substitute question OK, I'll try again See nothi.................................................. Late Kemlo Rogerson EX Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Education is not the filling of a pail, but the lighting of a fire. --W. B. Yeats This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Tue May 23 06:25:56 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Tue May 23 06:26:51 2006 Subject: [Histonet] Re: Bouins alternative/substitute question References: <20060522142556.gvmozwqlw808g4oc@mail.ucla.edu> Message-ID: Very interesting! I guess we'll have to give Gayle a break today...... ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of nienhuis@ucla.edu Sent: Mon 5/22/2006 5:25 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Bouins alternative/substitute question The article is available online if anyone wants to see it. Bob Nienhuis nienhuis@ucla.edu Quoting Carole Fields : > This is totally off the subject but.... I was reading an article in the May > 06 Smithsonian Magazine at my doctors office and noticed Gayle Callis's name > in the article about dinosaurs. It was a wonderful article about some > really exciting new discoveries in Montana. Everyone should read this > wonderful article. Congratulations on being involved in this exciting > project. > Carole Fields > Lexington, SC > > > -----Original Message----- > From: Gayle Callis [mailto:gcallis@montana.edu] > Sent: Monday, May 22, 2006 12:34 PM > To: Kemlo Rogerson; Histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Bouins alternative/substitute question > > > Kemlo, > > Interesting that you said this, and also a story from our university > chemical safety handling of dry picric acid found in an old > laboratory. They had a hard time getting it to explode also. > > Chemical safety removed a jar of picric acid, dry and age > undetermined. Knowing it was explosive, the fellow in charge took it out > to a very remote area and shot at the jar with a rifle, and it took a lot > of rifle bullets to get it to blow up. He did have a good session of > target practice for deer hunting season being in the Wild Wild West of > Montana. > > The potential is there for explosion, and we still have picric acid on our > shelves, although stored under a generous layer of distilled water. When > we need to make up Bouins, we just scoop it out and make sure we have a > saturated solution of picric acid. > > Care must be taken to keep dry crystals off lids, these are actually sealed > by dipping into hot paraffin, and check regularly for any leakage around > lid. I don't think it would be a good thing to have in a laboratory fire, > but solvents are just as bad. How many people still store isopentane in a > non explosion proof freezer - now that IS an explosive situation. > > We remain cautious but not in panic state. Bouins is still very > important as a fixative and mordant for Massons trichrome, we will continue > to use it, dispose of it correctly and not put metal cassettes into it. We > often purchase it ready made from Sigma to avoid having to handle it. > > At 09:27 AM 5/22/2006, you wrote: >> I think it is because picric acid explodes when dry but I've tried, and >> tried, and tried and it has never exploded. >> >> Has anyone ever succeeded? >> >> Kemlo Rogerson >> Pathology Manager >> Ext 3311 >> DD 01934 647057 >> Mob 07749 754194 >> >> Education is not the filling of a pail, but the lighting of a fire. --W. B. >> Yeats >> >> >> >> >> This e-mail is confidential and privileged. If you are not the intended >> recipient please accept my apologies; please do not disclose, copy or >> distribute information in this e-mail or take any action in reliance on its >> contents: to do so is strictly prohibited and may be unlawful. Please > inform >> me that this message has gone astray before deleting it. Thank you for your >> co-operation >> >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 > 406 994-4303 (FAX) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _________________________________ > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the email or any of its > attachments, please be advised that you have received this email in error > and that any use, dissemination, distribution, forwarding, printing, or > copying of this email or any attached files is strictly prohibited. If you > have received this email in error, please immediately purge it and all > attachments and notify the sender by reply email or contact the sender at > the number listed above if one is provided. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Julie.Sanders <@t> va.gov Tue May 23 07:17:35 2006 From: Julie.Sanders <@t> va.gov (Sanders, Julie, VHACIN) Date: Tue May 23 07:17:40 2006 Subject: [Histonet] Processing protocol for VIP Message-ID: <2D4ACE41DEFE93428F23D77988EFBCB50E5FD9@VHAV10MSGA2.v10.med.va.gov> Hi netters, I'm back with more questions about processing...We received our HQIP evaluation and everything was fine except for the breast and skin sections, both of which contained lots of soft tissue and fat. The comments were about "muddy nuclear detail, and hematoxylin not crisp..." Now the chief of my lab (a clinical pathologist) is up in arms suggesting that ALL of our work is sub-par. I cannot convince him that this is a problem with the type of tissue and fixation. So...with that said...anyone willing to share their processing protocols would be greatly appreciated. We have two processors, one for biopsies only, which we do not have a problem with, and the other for large cases..colons, breast, etc., it is a 12 hour protocol. I would be interested to see what everyone else is doing. Oh, we use VIP processors. Feel free to send to me personally if you wish, but I'm sure others on the net might be interested as well. Thanks in advance! Julie Julie Sanders, BA, HT(ASCP) Supervisor, Anatomic Pathology VAMC, Cincinnati, Ohio Julie.Sanders@va.gov From rjbuesa <@t> yahoo.com Tue May 23 07:23:04 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue May 23 07:23:09 2006 Subject: [Histonet] Uranyl Nitrate on Warthi Starry procedures In-Reply-To: <03B63DE44B5C014D84F9A9D8A968B5174C4D79@lithium.corp.gsopath.com> Message-ID: <20060523122304.29005.qmail@web61215.mail.yahoo.com> Gus: I substituted uranyl nitrate with phosphotungstic acid in the Steiner procedure. I did not try it in the Warthin Starry (that by the way I do not like that much). You could try this substitution. On separate cover I am attaching a paper I published on the subject that I hope will help you. Ren? J. Gus Mondragon wrote: Can someone tell me what to use in place of Uranyl Nitrate in the Warthin Starry procedures. Using this chemical is very unsafe as well very expensive to dispose of. Is there antibodies against the organism we can use in IHC. Gus Mondragon (gmondragon@gsopath.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Feel free to call! Free PC-to-PC calls. Low rates on PC-to-Phone. Get Yahoo! Messenger with Voice From KParker <@t> mail.nih.gov Tue May 23 08:24:45 2006 From: KParker <@t> mail.nih.gov (Tuttle, Kimberly (NIH/NCI) [E]) Date: Tue May 23 08:24:51 2006 Subject: [Histonet] FW: Save AFIP petition Message-ID: The message is ready to be sent with the following file or link attachments: Shortcut to: http://www.petitiononline.com/SaveAFIP/petition.html Note: To protect against computer viruses, e-mail programs may prevent sending or receiving certain types of file attachments. Check your e-mail security settings to determine how attachments are handled. From rjbuesa <@t> yahoo.com Tue May 23 08:55:43 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue May 23 08:55:46 2006 Subject: [Histonet] Processing protocol for VIP In-Reply-To: <2D4ACE41DEFE93428F23D77988EFBCB50E5FD9@VHAV10MSGA2.v10.med.va.gov> Message-ID: <20060523135543.21471.qmail@web61213.mail.yahoo.com> Hi Julie: If you get enough processing protocols, you will probably realize that there are as many protocols as laboratories there are; everybody has personal preferences. For tissue like you describe I always tried the following: 1- process the next day they were received, after a good 24 hours fixation; 2- pieces as thin as possible 3- a 15 hours processing without the first fixation step in the first 2 VIP stations: a- 60% ethanol and 80% ethanol: 1 station x 30 min each b- 95% ethanol x 1 hour c- 100% ethanol: 2 x 1 hour + 1 x 2 hours d- xylene x 1 hour e- xylene x 2 hours f- 4 paraffin stations: first 3 x 1 hour each + last x 2 hours TOTAL = 15 hour. Now the balance was: clear time / dehydration time = 3/7 or (clear + infiltration) / dehydraiton = 8/7 You could change the times but try to maintain those ratios. On June/99 I changed all my procedures to mineral oil (eliminated xylene) and "difficult" specimens (breast, colon, uterus, brain) began to process even better. If you want I can send you that procedure. I hope this will help you! Ren? J. "Sanders, Julie, VHACIN" wrote: Hi netters, I'm back with more questions about processing...We received our HQIP evaluation and everything was fine except for the breast and skin sections, both of which contained lots of soft tissue and fat. The comments were about "muddy nuclear detail, and hematoxylin not crisp..." Now the chief of my lab (a clinical pathologist) is up in arms suggesting that ALL of our work is sub-par. I cannot convince him that this is a problem with the type of tissue and fixation. So...with that said...anyone willing to share their processing protocols would be greatly appreciated. We have two processors, one for biopsies only, which we do not have a problem with, and the other for large cases..colons, breast, etc., it is a 12 hour protocol. I would be interested to see what everyone else is doing. Oh, we use VIP processors. Feel free to send to me personally if you wish, but I'm sure others on the net might be interested as well. Thanks in advance! Julie Julie Sanders, BA, HT(ASCP) Supervisor, Anatomic Pathology VAMC, Cincinnati, Ohio Julie.Sanders@va.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. From Jacqueline.Malam <@t> rli.mbht.nhs.uk Tue May 23 08:56:55 2006 From: Jacqueline.Malam <@t> rli.mbht.nhs.uk (Malam Jacqueline) Date: Tue May 23 08:58:23 2006 Subject: [Histonet] Cryostat disinfection Message-ID: We use Leica's cryofect - point and spray! Prior to that we used to fumigate with conc. formalin fumes overnight, neutralise those with ammonia, then disconnect the elec supply and remove microtome, wipe out and dry it and cabinet, re-assemble, plug back in - bit fiddly. Jacqui DISCLAIMER: This e-mail is confidential and privileged. If you are not the intended recipient please accept our apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform postmaster@rli.mbht.nhs.uk that this message has gone astray before deleting it. Comments or opinions expressed in this email are those of their respective contributors only. The views expressed do not represent the views of the Trust, its management or employees. University Hospitals of Morecambe Bay NHS Trust is not responsible and disclaims any and all liability for the content of comments written within.Thank you for your co-operation. From JWEEMS <@t> sjha.org Tue May 23 09:04:36 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue May 23 09:04:41 2006 Subject: [Histonet] Cryostat disinfection Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202736B55@sjhaexc02.sjha.org> If Leica folks are looking on - is this product ever going to be available in the US? Thanks! Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Malam Jacqueline Sent: Tuesday, May 23, 2006 9:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryostat disinfection We use Leica's cryofect - point and spray! Prior to that we used to fumigate with conc. formalin fumes overnight, neutralise those with ammonia, then disconnect the elec supply and remove microtome, wipe out and dry it and cabinet, re-assemble, plug back in - bit fiddly. Jacqui DISCLAIMER: This e-mail is confidential and privileged. If you are not the intended recipient please accept our apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform postmaster@rli.mbht.nhs.uk that this message has gone astray before deleting it. Comments or opinions expressed in this email are those of their respective contributors only. The views expressed do not represent the views of the Trust, its management or employees. University Hospitals of Morecambe Bay NHS Trust is not responsible and disclaims any and all liability for the content of comments written within.Thank you for your co-operation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From ldunikoski <@t> rbmc.org Tue May 23 09:10:56 2006 From: ldunikoski <@t> rbmc.org (Dunikoski, Leonard PhD) Date: Tue May 23 09:11:02 2006 Subject: [Histonet] Position Available (NJ) Message-ID: _____ Hospital system seeks supervisor for central anatomic pathology laboratory. The laboratory processes approximately 9,000 surgical and 3,000 cytology specimens per year. The candidate should be proficient in routine histologic techniques and immunohistochemistry. Duties will include supervision of technicians, compilation of laboratory statistics, and maintenance of current procedure manuals and maintenance records. _____ Leonard K. Dunikoski, Ph.D., DABCC, CHE Director of Operations, OBD Raritan Bay Medical Center 1 Hospital Plaza Old Bridge, NJ 08857 (732) 324-5163 Notice: The information included in this email contains confidential information belonging to the sender. This information is intended only for the use of the individual or entity named above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or action taken in reliance on the contents hereof is strictly prohibited. If you have received this email in error, please notify the sender immediately. From gcallis <@t> montana.edu Tue May 23 09:46:20 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue May 23 09:46:31 2006 Subject: [Histonet] more on antiFITC Re: rabbit anti-FITC In-Reply-To: References: <42a58b42bc32.42bc3242a58b@amc.uva.nl> Message-ID: <6.0.0.22.1.20060523084145.01b48e50@gemini.msu.montana.edu> I also found a goat antiFITC from ABCAM. At 11:01 PM 5/22/2006, you wrote: >--> >Thanks for the heads up Chris ... Incidentially .... Has anyone tried >Zymed's Rabbit anti-FITC? > >Cheers, >Andrea Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 From akbitting <@t> geisinger.edu Tue May 23 09:51:55 2006 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Tue May 23 09:52:19 2006 Subject: [Histonet] Processing protocol for VIP Message-ID: On the same subject..HistoQIP- I had a CD20 stain that was graded deficient. My understanding of this program is that it is educational only. So do these deficiencies have to be formally addressed like Proficiency Testing surveys? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From JCLARK <@t> kumc.edu Tue May 23 09:58:03 2006 From: JCLARK <@t> kumc.edu (Julie Clark) Date: Tue May 23 09:58:14 2006 Subject: [Histonet] DNA/RNA Extraction Kits for paraffinised tissues Message-ID: Shirley, Qiagen has kits for FFPE tissue that I have used. We have had good results with them. I would recommend an overnight digestion step rather than the 2-3 hours they recommend. I have also used kits from Arcturus (www.arctur.com) with LCM samples with good results (see Brian Petroff's success story for our work). Julie Clark Supervisor, Molecular Pathology Laboratory Department of Pathology Room 2000 Hixon Mailstop 3045 Univ. of Kansas Med. Center Kansas City, KS 66160 Office: 913-588-7254 Lab: 913-588-1793 Page: 913-917-6052 FAX: 913-588-7073 >>> Shirley PHUA 05/22/06 9:31 PM >>> Dear All Have anyone heard of any DNA/RNA extraction kits for formalin-fixed, paraffinised tissues? I will greatly appreciate if you can share some of your feedbacks. Were your yields good? Thanks in advance! Shirley Phua Histopathology Laboratory Centre for Forensic Medicine Health Sciences Authority DID : 62130654 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Tue May 23 10:03:15 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue May 23 10:02:56 2006 Subject: [Histonet] tyrosine hydroxylase staining?? In-Reply-To: <2A1F3E27491CBF46BC14ACB3E5C7D89AB55B74@icex3.ic.ac.uk> References: <2A1F3E27491CBF46BC14ACB3E5C7D89AB55B74@icex3.ic.ac.uk> Message-ID: <44732433.9050106@umdnj.edu> Hi Johanna: Your proceedure sounds fine to me. I have never used the antibody in question but getting good results with TH should be VERY easy. I use a polyclonal TH from Pel-Freez in Rogers, AK, works great on both mouse and rat brains. Assuming the brains were not overfixed (24 hours could be too long) and the antibody is still good I would make sure all of my solutions were properly labeled. Also, is your detection system OK? You could have great binding but if the detection system is not working properly you will never know it. Geoff Jackson, Johanna wrote: >Dear All, > >I am trying to stain dopaminergic neurons in the striatum of the adult rat brain which has been perfused with 4% PFA, washed and then cryoprotected in 20% sucrose, before being sectioned (10um) on a cryostat. I pretreat the sections with 0.1% Triton-X and block using serum. I am using the monoclonal anti-tyrosine hydroxylase antibody from Sigma (T1299). Sigma recommend a working concentration of 1:2000 however I have had no luck with this staining. I just a weak staining all over the brain with no specificity in the striatum. > >Does anyone have a protocol using this particular antibody? I have seen protocols with anti-TH from other companies which follow a similar method to what I have described above, however they do not seem to work for the Sigma antibody. > >Any help would be greatly appreciated! > >Thanks! > >Jo > > >Stem Cell Imaging >MRC Clinical Sciences Centre >Imperial College London >Hammersmith Hospital Campus >Du Cane Road >London >W12 0NN >0208 3833796 >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From gcallis <@t> montana.edu Tue May 23 10:04:21 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue May 23 10:04:31 2006 Subject: [Histonet] Cryostat disinfection In-Reply-To: References: Message-ID: <6.0.0.22.1.20060523090325.01b90e98@gemini.msu.montana.edu> I believe Cryofect is cleared for use in Europe, UK but not in the USA. Leica can clarify that. At 07:56 AM 5/23/2006, you wrote: >We use Leica's cryofect - point and spray! Prior to that we used to fumigate >with conc. formalin fumes overnight, neutralise those with ammonia, then >disconnect the elec supply and remove microtome, wipe out and dry it and >cabinet, re-assemble, plug back in - bit fiddly. > >Jacqui > Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From mcauliff <@t> umdnj.edu Tue May 23 09:06:05 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue May 23 10:05:39 2006 Subject: [Histonet] Incubation chamber In-Reply-To: <20060517073114.43973.qmail@web32914.mail.mud.yahoo.com> References: <20060517073114.43973.qmail@web32914.mail.mud.yahoo.com> Message-ID: <447316CD.6060008@umdnj.edu> I use "Tupperware", or a generic equivalent. Slides are supported by glass rods in the bottom of the container. Add a little water and seal. Geoff Jatindra Prakash wrote: >Does anyone know of a supplier for a low cost incubation chambers for hold up to 6 slides? >I am tired of having slides dry out during overnight incubation in the make-shift chambers >I have tried to construct. > >Thanks in advance, > >Jatindra >Atlantic Veterinary College >Canada > > > >--------------------------------- >How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From dusko.trajkovic <@t> pfizer.com Tue May 23 10:16:59 2006 From: dusko.trajkovic <@t> pfizer.com (Trajkovic, Dusko) Date: Tue May 23 10:17:09 2006 Subject: [Histonet] Position available in So. California for EM tech Message-ID: <3AD0BD3142459B4E9B12CBEAFF2B89B202E9141C@lajamrexm01.amer.pfizer.com> I'm posting this for a friend of mine. Please respond directly to Ulrich Mueller. Thank you Dusko Trajkovic ELECTRON MICROSCOPIST A full-time position is available at The Scripps Research Institute (http://www. scripps.edu) in the Department of Cell Biology for a Research Technician with expertise in electron microscopy. The laboratory studies the mechanisms of sound perception and defects in this process that cause deafness (http://www.scripps.edu/cb/mueller/). We generate and analyze mice genetically engineered to mimic human diseases that cause deafness. The technician will join a highly motivated team consisting of research technicians, Ph.D. students and postdoctoral fellows. The salary will depend on the level of experience and includes full benefits. Applications should be addressed to Dr. Ulrich Mueller and can be submitted by e-mail (umueller@scripps.edu) or regular mail (Dr. Ulrich Mueller, The Scripps Research Institute, Department of Cell Biology, 10550 N. Torrey Pines Rd, Mail Drop ICND222, La Jolla, Ca 92037). -- Ulrich Mueller, Ph.D. The Scripps Research Institute 10550 N. Torrey Pines Rd. Mail Drop ICND222 La Jolla, California 92037 T:858-784-7288 F:858-784-7299 umueller@scripps.edu http://www.scripps.edu/cb/mueller/ ---------------------------------------------------------------------- LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately. From gcallis <@t> montana.edu Tue May 23 10:21:47 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue May 23 10:21:57 2006 Subject: Source of Re: [Histonet] Incubation chamber In-Reply-To: <447316CD.6060008@umdnj.edu> References: <20060517073114.43973.qmail@web32914.mail.mud.yahoo.com> <447316CD.6060008@umdnj.edu> Message-ID: <6.0.0.22.1.20060523091730.01b926b0@gemini.msu.montana.edu> Evergreen Scientific has a 10 slide chamber, plastic, with lid. The chamber is designed so slides are elevated, just add water to bottom of wells for humidity. There are 3 chambers per package, very inexpensive. You can stack them. They come either black plastic for fluorescence work OR clear. I have seen them sold other places to, maybe EMS is the place. Evergreen makes them. Very tidy little devices for low volume work At 08:06 AM 5/23/2006, you wrote: >I use "Tupperware", or a generic equivalent. Slides are supported by glass >rods in the bottom of the container. Add a little water and seal. > >Geoff Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From Tracey.Lenek <@t> CLS.ab.ca Tue May 23 10:28:35 2006 From: Tracey.Lenek <@t> CLS.ab.ca (Tracey.Lenek@CLS.ab.ca) Date: Tue May 23 10:28:39 2006 Subject: [Histonet] Hoescht stain Message-ID: <4BC300747AF87A48BCDF8E48BC2885CE02C1D7C2@mail1.calgary.com> Hi, We are doing an immunofluorescence research project for podocytes. The urine cytospins are to be counterstained with Hoescht. We have never used it - can someone recommend a concentration, diluent and staining time that we could start with. Thanks in advance. Tracey CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. From anh2006 <@t> med.cornell.edu Tue May 23 11:18:38 2006 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Tue May 23 11:17:51 2006 Subject: [Histonet] Incubation chamber In-Reply-To: <6.0.0.22.1.20060523091730.01b926b0@gemini.msu.montana.edu> References: <20060517073114.43973.qmail@web32914.mail.mud.yahoo.com> <447316CD.6060008@umdnj.edu> <6.0.0.22.1.20060523091730.01b926b0@gemini.msu.montana.edu> Message-ID: Along those same lines, plastic slide boxes also work famously well for small runs. Just place the slides ontop of the slide holder slots and some water or buffer beneath and voila, a humid chamber! At 9:21 AM -0600 5/23/06, Gayle Callis wrote: >Evergreen Scientific has a 10 slide chamber, plastic, with lid. The >chamber is designed so slides are elevated, just add water to bottom >of wells for humidity. There are 3 chambers per package, very >inexpensive. You can stack them. They come either black plastic >for fluorescence work OR clear. I have seen them sold other places >to, maybe EMS is the place. Evergreen makes them. > >Very tidy little devices for low volume work > >Gayle Callis -- From lyonm <@t> upstate.edu Tue May 23 11:30:27 2006 From: lyonm <@t> upstate.edu (Michael J. Lyon, Ph.D.) Date: Tue May 23 11:30:44 2006 Subject: [Histonet] DNA/RNA Extraction Kits for paraffinised tissues In-Reply-To: Message-ID: <006101c67e86$32de3a60$31ae7f8b@lyonoffice> I just receive information from Stratagene. They have a kit "Absolutely RNA FFPE Kit". www.statagene.com I have not used it so have no knowledge to its usefulness. Mike > -----Original Message----- > From: louise renton [mailto:louise.renton@gmail.com] > Sent: Tuesday, May 23, 2006 4:36 AM > To: Shirley PHUA > Cc: Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] DNA/RNA Extraction Kits for paraffinised tissues > > Dear Shirley, > > My information may be somewhat out of date as it is several years > since I did DNA extration on formalin fixed /praffin embedded tissues, > but at that time I found that the commercial kits (specifically the > ones using spin columns) were not as good as the old fashioned method > of phenol chloroform. > > The amount/quality of DNA available is directly related to the type > and length of fixation so harsh unbuffered fixation will degrade the > DNA. > > This is my 2 cents worth > > > On 5/23/06, Shirley PHUA wrote: > > Dear All > > > > Have anyone heard of any DNA/RNA extraction kits for formalin-fixed, > > paraffinised tissues? I will greatly appreciate if you can share some of > > your feedbacks. Were your yields good? > > > > Thanks in advance! > > > > Shirley Phua > > Histopathology Laboratory > > Centre for Forensic Medicine > > Health Sciences Authority > > DID : 62130654 > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > -- > Louise Renton > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > "There are nights when the wolves are silent and only the moon howls". > George Carlin > From gu.lang <@t> gmx.at Tue May 23 12:32:18 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue May 23 12:32:26 2006 Subject: [Histonet] Formalin on skin Message-ID: <000101c67e8e$d7693550$eeeea8c0@SERVER01> Hi, Today a labassistent asked me, what would happen to her skin, if formalin swallowed over her arm. I said, the formalin would harden the skin, but only on the surface. She should wash the arm with plenty of water, and there will reamain no negative reaction. It would not be dangerous for her health. Do you agree with me? Is there a cancer-risk after short contact with formalin? Greetings Gudrun Lang Histolab Akh Linz Austria From pdc96 <@t> hotmail.com Tue May 23 12:35:58 2006 From: pdc96 <@t> hotmail.com (Paul Cremers) Date: Tue May 23 12:36:08 2006 Subject: [Histonet] (no subject) Message-ID: Hello to all, Is there anyone looking for a histotech or two. My wife and I are wanting to move to the Boulder or Fort Collins, Colorado area. We are also interested in Portland, Salem, Corvalis and Eugene, Oregon. We are both HT certified and are wanting to start somewhere between mid-August and September 1. Thanks, Paul and Melissa Cremers From Charles.Embrey <@t> carle.com Tue May 23 12:56:15 2006 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Tue May 23 12:56:23 2006 Subject: [Histonet] Formalin on skin Message-ID: You are 100% correct. I have even reached a bare hand into formalin to pull out something before but washed with soap and water minutes after. I have not personally heard of formalin causing an increased risk of skin cancer however I am sure that there is a study somewhere that has probably associated some risk with L---O---N---G term exposure. Your labassistant should be perfectly safe. Just don't drink it and keep it out of the eyes :) Charles Embrey Jr. PA(ASCP) www.greyrealm.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Tuesday, May 23, 2006 12:32 PM To: Histonetliste (Histonetliste) Subject: [Histonet] Formalin on skin Hi, Today a labassistent asked me, what would happen to her skin, if formalin swallowed over her arm. I said, the formalin would harden the skin, but only on the surface. She should wash the arm with plenty of water, and there will reamain no negative reaction. It would not be dangerous for her health. Do you agree with me? Is there a cancer-risk after short contact with formalin? Greetings Gudrun Lang Histolab Akh Linz Austria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Shirley_PHUA <@t> hsa.gov.sg Tue May 23 13:11:23 2006 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Tue May 23 13:11:37 2006 Subject: [Histonet] Shirley on leave : 24 May 2006 (Wednesday) Message-ID: I will be out of the office from 24/05/2006 to 24/05/2006. I am away on 1 day leave. I will return on 25/05/2006 (Thursday). Pathologists : I will process your requests when I return. Otherwise, if urgent, please contact Henry. From llewllew <@t> shaw.ca Tue May 23 14:12:39 2006 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Tue May 23 14:12:50 2006 Subject: [Histonet] Formalin on skin References: Message-ID: <001201c67e9c$dbefc680$130e4246@yourlk4rlmsu> Most of us old-timers have stuck our fingers in formalin or spilled it on ourselves on many occasions. I have never heard of anyone developing a tumour from it, though. It certainly doesn't appear to be common if it does happen. In Canada some years ago we had a government program assisting people to inject urea-formaldehyde resin into the walls of houses as a retrofit insulation system. A few years later it was decided the formalin fumes given off were carcinogenic, so we had another program to remove it. That's about the only link to tumours I have heard of. One of the pathologists I used to work with many years ago was fond of quoting a paper he had once read which compared naso-pharyngeal tumour rates among various groups. Pathologists had a lower rate than other people. He always put that down to the formalin fumes they breathed in all the time. The real identified problem with formalin and skin contact is dermatitis. It isn't universal, but some people do get it with repeated contact, and once you develop the sensitivity to it, it doesn't go away. So wear gloves. Bryan Llewellyn ----- Original Message ----- From: "Charles.Embrey" To: ; "Histonetliste (Histonetliste)" Sent: Tuesday, May 23, 2006 10:56 AM Subject: RE: [Histonet] Formalin on skin You are 100% correct. I have even reached a bare hand into formalin to pull out something before but washed with soap and water minutes after. I have not personally heard of formalin causing an increased risk of skin cancer however I am sure that there is a study somewhere that has probably associated some risk with L---O---N---G term exposure. Your labassistant should be perfectly safe. Just don't drink it and keep it out of the eyes :) Charles Embrey Jr. PA(ASCP) www.greyrealm.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Tuesday, May 23, 2006 12:32 PM To: Histonetliste (Histonetliste) Subject: [Histonet] Formalin on skin Hi, Today a labassistent asked me, what would happen to her skin, if formalin swallowed over her arm. I said, the formalin would harden the skin, but only on the surface. She should wash the arm with plenty of water, and there will reamain no negative reaction. It would not be dangerous for her health. Do you agree with me? Is there a cancer-risk after short contact with formalin? Greetings Gudrun Lang Histolab Akh Linz Austria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cpomajzl <@t> cpllabs.com Tue May 23 13:51:49 2006 From: cpomajzl <@t> cpllabs.com (Chris Pomajzl) Date: Tue May 23 14:23:18 2006 Subject: [Histonet] Formalin on skin References: Message-ID: <004301c67e99$f31fa030$26fca8c0@CSP> I would imagine that if someone had open wounds or breaks in the skin, there could be acute problems. But chronic long-term problems are unlikely with such a brief incident. People drink formaldehyde (or at least used to, wasn't it a preservative in beer many years ago??), they smoke it (I've heard of people dipping joints in formaldehyde for a stronger buzz, never tried it myself), and we breathe it in the lab every day. I believe that the carcinogenic effects are due to long-term exposure in laboratory animals. I am not aware of any studies involving human subjects. Hope this helps. ----- Original Message ----- From: "Charles.Embrey" To: ; "Histonetliste (Histonetliste)" Sent: Tuesday, May 23, 2006 12:56 PM Subject: RE: [Histonet] Formalin on skin You are 100% correct. I have even reached a bare hand into formalin to pull out something before but washed with soap and water minutes after. I have not personally heard of formalin causing an increased risk of skin cancer however I am sure that there is a study somewhere that has probably associated some risk with L---O---N---G term exposure. Your labassistant should be perfectly safe. Just don't drink it and keep it out of the eyes :) Charles Embrey Jr. PA(ASCP) www.greyrealm.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Tuesday, May 23, 2006 12:32 PM To: Histonetliste (Histonetliste) Subject: [Histonet] Formalin on skin Hi, Today a labassistent asked me, what would happen to her skin, if formalin swallowed over her arm. I said, the formalin would harden the skin, but only on the surface. She should wash the arm with plenty of water, and there will reamain no negative reaction. It would not be dangerous for her health. Do you agree with me? Is there a cancer-risk after short contact with formalin? Greetings Gudrun Lang Histolab Akh Linz Austria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Tue May 23 15:30:39 2006 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Tue May 23 15:31:23 2006 Subject: [Histonet] re:Hoechst staining... Message-ID: Of nuclei! Easy.....If you wish, check out this site for a protocol and images http://www.immunoportal.com/index.php Saves me fingers retyping ;-) It's a standard, tho'. No fancy stuff reqd. Lots of XPerienced people here to help, that I will always pay greatest respects to. Regards From Jackie.O'Connor <@t> abbott.com Tue May 23 15:32:56 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Tue May 23 15:33:29 2006 Subject: [Histonet] Formalin on skin In-Reply-To: <004301c67e99$f31fa030$26fca8c0@CSP> Message-ID: Formaldehyde was a common ingredient of shampoos, and I remember reading the ingredients (with horror) on a bottle of Mr. Bubble bubble bath for kids (like 25 years ago). If you remember "Good Morning Vietnam" - they mentioned using formaldehyde to produce a better head on a glass of beer from the tap. I once worked with a pathologist who was allergic to formaldehyde. When he did the gross, we had to rinse all the specimens in H20 before he could examine them - yeah, that was fun. Jackie O' "Chris Pomajzl" Sent by: histonet-bounces@lists.utsouthwestern.edu 05/23/2006 01:51 PM To "HISTONET" cc Subject Re: [Histonet] Formalin on skin I would imagine that if someone had open wounds or breaks in the skin, there could be acute problems. But chronic long-term problems are unlikely with such a brief incident. People drink formaldehyde (or at least used to, wasn't it a preservative in beer many years ago??), they smoke it (I've heard of people dipping joints in formaldehyde for a stronger buzz, never tried it myself), and we breathe it in the lab every day. I believe that the carcinogenic effects are due to long-term exposure in laboratory animals. I am not aware of any studies involving human subjects. Hope this helps. ----- Original Message ----- From: "Charles.Embrey" To: ; "Histonetliste (Histonetliste)" Sent: Tuesday, May 23, 2006 12:56 PM Subject: RE: [Histonet] Formalin on skin You are 100% correct. I have even reached a bare hand into formalin to pull out something before but washed with soap and water minutes after. I have not personally heard of formalin causing an increased risk of skin cancer however I am sure that there is a study somewhere that has probably associated some risk with L---O---N---G term exposure. Your labassistant should be perfectly safe. Just don't drink it and keep it out of the eyes :) Charles Embrey Jr. PA(ASCP) www.greyrealm.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Tuesday, May 23, 2006 12:32 PM To: Histonetliste (Histonetliste) Subject: [Histonet] Formalin on skin Hi, Today a labassistent asked me, what would happen to her skin, if formalin swallowed over her arm. I said, the formalin would harden the skin, but only on the surface. She should wash the arm with plenty of water, and there will reamain no negative reaction. It would not be dangerous for her health. Do you agree with me? Is there a cancer-risk after short contact with formalin? Greetings Gudrun Lang Histolab Akh Linz Austria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Tue May 23 16:55:27 2006 From: shive003 <@t> umn.edu (Jan Shivers) Date: Tue May 23 16:55:34 2006 Subject: Fw: Source of Re: [Histonet] Incubation chamber Message-ID: <012801c67eb3$9a32e3f0$a1065486@auxs.umn.edu> Years ago when my IHC lab was a fledgling entity with very little budget, I used a 9x13" metal cake pan (with lid), wet paper towels for humidity in the bottom, and disposable 1 ml pipets as the slide racks. They held two rows of 10 slides each. Just had to make sure my wet paper towels laid flat (no bubbles), otherwise they'd make the pipets sit uneven. Jan Shivers UMN VDL ----- Original Message ----- From: "Gayle Callis" To: "Geoff McAuliffe" ; Sent: Tuesday, May 23, 2006 10:21 AM Subject: Source of Re: [Histonet] Incubation chamber > Evergreen Scientific has a 10 slide chamber, plastic, with lid. The > chamber is designed so slides are elevated, just add water to bottom of > wells for humidity. There are 3 chambers per package, very inexpensive. > You can stack them. They come either black plastic for fluorescence work > OR clear. I have seen them sold other places to, maybe EMS is the place. > Evergreen makes them. > > Very tidy little devices for low volume work > > At 08:06 AM 5/23/2006, you wrote: >>I use "Tupperware", or a generic equivalent. Slides are supported by glass >>rods in the bottom of the container. Add a little water and seal. >> >>Geoff > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 > 406 994-4303 (FAX) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From POWELL_SA <@t> Mercer.edu Tue May 23 17:00:28 2006 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Tue May 23 17:00:50 2006 Subject: [Histonet] GSH meeting Message-ID: <01M2RQRHLNHW8WWEFM@Macon2.Mercer.edu> Reminder:::: The Georgia Society for Histotechnology and the Georgia Society of Cytology will hold their 4th Annual Combined Scientific Symposium on Saturday, June 3, 2006, in Augusta, Georgia at the Marriott Augusta Hotel and Suites, 2 Tenth Street, Augusta, GA 30901. The phone number for reservations is 1-706 722-8900. I will email you a program upon request. I also have vendor information for any who wish to exhibit. Thanks, Shirley Powell GSH Secretary/Membership Chair From ploykasek <@t> phenopath.com Tue May 23 17:39:28 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Tue May 23 17:39:36 2006 Subject: [Histonet] STAFF EVALUATIONS In-Reply-To: Message-ID: Hi Diana. I would be interested in your sharing the info that you get back. I can tell you what we do. I'll start off by saying we are constantly updating/revising our program. I feel like we do a better job each year. Some tasks are evaluated by observation & documented on a spreadsheet that contains all SOPs (by name) that a particular staff should know. I do have checklists for equipment that cover things from do staff know where on/off is, to the various functions of the equipment staff should know. For the histologists we evaluate H&E sections (both paraffin & FS) with criteria similar to the board registry & include some safety & clean up details. I'm putting together a test to cover knowledge of general IHC & would like to get some photos of common stains acceptable & not for a test. A work in progress - always. Hope that helps a bit. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Does anyone do an annual evaluation of staff ability to perform certain tasks? > Diana McCaig 519-352-6401 (6604) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From kerry.l.crabb <@t> gsk.com Tue May 23 17:45:19 2006 From: kerry.l.crabb <@t> gsk.com (kerry.l.crabb@gsk.com) Date: Tue May 23 17:45:35 2006 Subject: [Histonet] Sealing Paraffin Blocks Message-ID: What is the current opinion and practice on sealing paraffin blocks prior to filing/archiving them? What is the pro and con in your opinion? From ploykasek <@t> phenopath.com Tue May 23 17:46:19 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Tue May 23 17:46:32 2006 Subject: [Histonet] Re: Fixatives In-Reply-To: <41a.1ecaee5.31a42052@aol.com> Message-ID: Dr. Richmond, I always appreciate your views and comments. I do think if a bottle comes to the lab with a label noting fixative it could be dictated as "...received in container labeled ..." or ",,,received in unlabeled container..", this is what was done at previous facilities I have worked, but perhaps is not the norm. I do agree that the elephant in the room is how poor or improper fixation/processing affects everything downstream. I could go on & on about this issue. I feel that CAP nor anyone else does very little to address this issue. Everyone turns a bit of a blind eye to it. Thanks for the input. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Patti Loykasek at PhenoPath Laboratories notes >>We are a reference lab and > receive specimens from all over the USA. One of my "pet peeves" is that it is > rare to see in the report exactly what type of fixative the specimen was > received in or subsequently processed in. I know we have no standard form of > reporting, but it just seems like best practice to me to include this > information on > the report. One of my favorites is "...received in fixative..." - not very > helpful.<< > > That's exactly the phrase I use in my gross descriptions, and for a very good > reason. I'm not about to stick my nose into every specimen bottle to verify > that it contains formalin and not alcohol, water, or pine-scented floor > disinfectant (used at one hospital I know as a "fixative" for placentas). I'm > willing > to smell-test a very occasional container where I'm suspicious that the wrong > fixative has been used, but not every time! > > Time of fixation is the dead horse in the middle of the living room floor - > nobody wants to hear that the HER2 immunostain for breast cancer requires > overnight fixation, for example. > > Bob Richmond > Gastonia NC > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From Nalini.Makhijani <@t> va.gov Tue May 23 19:09:02 2006 From: Nalini.Makhijani <@t> va.gov (Makhijani, Nalini S) Date: Tue May 23 19:09:11 2006 Subject: [Histonet] DNA/RNA Extraction Kits for paraffinised tissues Message-ID: <715FA772CEA81A47B1A762AB6E45123A0111B549@VHAV22MSGA3.v22.med.va.gov> Ambion advertises a kit, "RecoverAll", for total RNA and DNA extraction from FFPE tissues. I've never used it, but you could get more information from their web site: www.ambion.com/prod/recoverall. Hope this helps! Nalini Makhijani, M.S. Research Biologist VA Greater Los Angeles Healthcare System -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley PHUA Sent: Monday, May 22, 2006 7:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DNA/RNA Extraction Kits for paraffinised tissues Dear All Have anyone heard of any DNA/RNA extraction kits for formalin-fixed, paraffinised tissues? I will greatly appreciate if you can share some of your feedbacks. Were your yields good? Thanks in advance! Shirley Phua Histopathology Laboratory Centre for Forensic Medicine Health Sciences Authority DID : 62130654 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kaleid11 <@t> yahoo.com Tue May 23 19:17:35 2006 From: kaleid11 <@t> yahoo.com (Adam Perry) Date: Tue May 23 19:17:39 2006 Subject: [Histonet] tyrosine hydroxylase staining?? In-Reply-To: <44732433.9050106@umdnj.edu> Message-ID: <20060524001735.22829.qmail@web30413.mail.mud.yahoo.com> Hey Johanna, Just curious...when you say you get weak, diffuse staining all over the brain, is that in sections that should contain dopaminergic cell bodies (like in the midbrain, hypothalamus, etc.) or in areas where you would mostly see diffuse fiber staining (like in the striatum)? Because I've started staining for TH in rodent brain and the staining pattern in the striatum is diffuse (i.e. the whole striatum is darker than the surrounding cortex, but nothing like cell bodies...because it's mostly terminal projections- correct me if I'm wrong, but I wouldn't expect to see many/any cell bodies labeled with TH in the striatum), whereas it clearly picks up cell bodies in regions that contain dopamine producing neurons. So if you were only staining rostral forebrain sections (like the striatum), I don't know if you should expect to see cell bodies, just diffuse fibers. If you're not already doing it, maybe you should include more caudal sections in your IHC (sections containing the arcuate nucleus or ventral tegmental area, etc. would be great)...that way you'd know if you're detecting cells in those areas and the weak staining in striatum could be fibers that just need amplification? Sorry if I'm misinterpreting your post about the weak staining...but it would be an easy thing to overlook if you didn't have sections with cell bodies in them... Finally, I've only used a sheep anti-TH IgG from Novus Biological and it worked beautifully on the first time in a non-traditional rodent species (1:1,000 with fluorescence, so I imagine even more dilute concentrations should work with enzyme-mediated chromagen detection)...so I agree with Geoff McAuliffe that getting good results with TH should be easy... If you're interested I can email you my protocol, it's not too different from what you described....but what detection system are you using? Fluorescence, DAB, other? Because you didn't mention any pretreatments like HRP inactivation or quenching unreacted aldehydes...maybe if you give more particulars to your methods, others could provide more tips? Good luck with the trouble shooting, Adam Geoff McAuliffe wrote: Hi Johanna: Your proceedure sounds fine to me. I have never used the antibody in question but getting good results with TH should be VERY easy. I use a polyclonal TH from Pel-Freez in Rogers, AK, works great on both mouse and rat brains. Assuming the brains were not overfixed (24 hours could be too long) and the antibody is still good I would make sure all of my solutions were properly labeled. Also, is your detection system OK? You could have great binding but if the detection system is not working properly you will never know it. Geoff Jackson, Johanna wrote: >Dear All, > >I am trying to stain dopaminergic neurons in the striatum of the adult rat brain which has been perfused with 4% PFA, washed and then cryoprotected in 20% sucrose, before being sectioned (10um) on a cryostat. I pretreat the sections with 0.1% Triton-X and block using serum. I am using the monoclonal anti-tyrosine hydroxylase antibody from Sigma (T1299). Sigma recommend a working concentration of 1:2000 however I have had no luck with this staining. I just a weak staining all over the brain with no specificity in the striatum. > >Does anyone have a protocol using this particular antibody? I have seen protocols with anti-TH from other companies which follow a similar method to what I have described above, however they do not seem to work for the Sigma antibody. > >Any help would be greatly appreciated! > >Thanks! > >Jo > > >Stem Cell Imaging >MRC Clinical Sciences Centre >Imperial College London >Hammersmith Hospital Campus >Du Cane Road >London >W12 0NN >0208 3833796 >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. PC-to-Phone calls for ridiculously low rates. From katri <@t> cogeco.ca Tue May 23 22:01:54 2006 From: katri <@t> cogeco.ca (Katri Tuomala) Date: Tue May 23 22:01:53 2006 Subject: [Histonet] Re: Fixatives References: <41a.1ecaee5.31a42052@aol.com> Message-ID: <006e01c67ede$69e28450$6a9a9618@Katri> I have always read with respect Dr. Richmond's opinions, but here I have to disagree. Surely the bottle, that the specimen comes in, is labelled with what ever the fixative is, along with the pertinent patient information. It should be just as simple to say " specimen received in formalin (Bouins, Zenkers etc.)". There is no need and it is not advisable to smell it. It is an interesting mindset among many, not all, pathologist to insist in processing all tissues the same day (TAT!) ending up with inadequately fixed and consequently poorly processed tissue, which we technologist then have to struggle to produce well stained, wrinkle free, representative sections. As an immuno tech I can testify for even bigger problems in producing reliable results with immunohistochemical procedures. For purposes of immunostaining, it is important to know the time of fixation in formalin, adequate or not (24 hours is considered adequate, not 4 or even 8), so that we know what we are faced with and sometimes method can be adjusted to fit the fixation time. I would prefer doing immuno stains on a specimen, that is "overfixed" in formalin rather than underfixed. If I ever ended up with breast cancer, I would make sure, that at least one tumor section got fixed 24 hours. This is my Tuesday Rant! Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: To: Sent: Tuesday, May 23, 2006 4:22 AM Subject: [Histonet] Re: Fixatives > Patti Loykasek at PhenoPath Laboratories notes >>We are a reference lab > and > receive specimens from all over the USA. One of my "pet peeves" is that it > is > rare to see in the report exactly what type of fixative the specimen was > received in or subsequently processed in. I know we have no standard form > of > reporting, but it just seems like best practice to me to include this > information on > the report. One of my favorites is "...received in fixative..." - not very > helpful.<< > > That's exactly the phrase I use in my gross descriptions, and for a very > good > reason. I'm not about to stick my nose into every specimen bottle to > verify > that it contains formalin and not alcohol, water, or pine-scented floor > disinfectant (used at one hospital I know as a "fixative" for placentas). > I'm willing > to smell-test a very occasional container where I'm suspicious that the > wrong > fixative has been used, but not every time! > > Time of fixation is the dead horse in the middle of the living room > floor - > nobody wants to hear that the HER2 immunostain for breast cancer requires > overnight fixation, for example. > > Bob Richmond > Gastonia NC > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo.rogerson <@t> waht.swest.nhs.uk Wed May 24 02:01:59 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed May 24 02:01:44 2006 Subject: [Histonet] Formalin on skin Message-ID: Interestingly fish medication contains malachite green and formalin to cure ulcers and to kill parasites; though oddly I think they are caused by the same thing. Fish harbor aeromonas and under extremes succumb to ulcers; on recovery invariably flagellated protozoa infest the wound and keep it 'open', the fish waterlogs. Formalin kills these parasites but not as well as metronidazole; though getting the medicine can be embarrassing. Have I gone off piste? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Education is not the filling of a pail, but the lighting of a fire. --W. B. Yeats This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From kemlo.rogerson <@t> waht.swest.nhs.uk Wed May 24 02:06:24 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed May 24 02:06:10 2006 Subject: [Histonet] Formalin on skin Message-ID: Whilst I accept your premise that the mutagenic properties of formalin is low; anything that causes cell death, and formalin certainly does that, increases the mitotic activity of replacement cells and thereby increases the risk of an abnormality. Personally I have excluded pregnant woman from the presence of formalin and try to have zero tolerance for trying to fix my Staff in its fumes by using extraction, monitoring and protective gloves, etc. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Education is not the filling of a pail, but the lighting of a fire. --W. B. Yeats This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From kemlo.rogerson <@t> waht.swest.nhs.uk Wed May 24 02:09:43 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed May 24 02:09:36 2006 Subject: [Histonet] Formalin on skin Message-ID: Maybe it's not the physical drenching of the skin and the effects on that you should worry about but the fact that formalin is now on a larger warm surface area and the known dangers of formalin inhalation on the lung; best not to experiment on Staff IMHO. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From ctsblack <@t> capeheart.uct.ac.za Wed May 24 04:39:19 2006 From: ctsblack <@t> capeheart.uct.ac.za (Melanie Black) Date: Wed May 24 04:45:07 2006 Subject: [Histonet] RE: Hoechst staining method Message-ID: Hi Tracey Some years ago, I looked into fluorescent nuclear counterstains, including dapi, propidium iodide and HOECHST. Heochst is also known as bisBenzimide. Method: Mix 1mg bisBemzimide (Sigma B2883) with 1ml Dist water. Take 4.0 micro litres of this stock and dilute with 1ml PBS pH 7.4.Apply to sections for 10 mins, wash 3 x in PBS. Fluorescence is bright blue, viewed with a UV filter 340 nm. Stain reference: Latt,SA and Stetten GJ. Histochem. Cytochem 24,24 (1976) Araki, T et al, Histochem. 87,331 (1987) Regards Melanie Black Hi, We are doing an immunofluorescence research project for podocytes. The urine cytospins are to be counterstained with Hoescht. We have never used it - can someone recommend a concentration, diluent and staining time that we could start with. Thanks in advance. Tracey -- Cardiovascular Research Unit Div. of Cardiothoracic Surgery Chris Barnard Building University of Cape Town Anzio Road Observatory 7925 Republic of South Africa Tel +27 21 406-6589 Cel +27 82 469-3352 Fax +27 21 448-5935 From JWEEMS <@t> sjha.org Wed May 24 06:17:18 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed May 24 06:21:36 2006 Subject: [Histonet] Re: Fixatives References: <41a.1ecaee5.31a42052@aol.com> <006e01c67ede$69e28450$6a9a9618@Katri> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD320263E103@sjhaexc02.sjha.org> "Surely the bottle, that the specimen comes in, is labelled with what ever the fixative is, along with the pertinent patient information. It should be just as simple to say " specimen received in formalin (Bouins, Zenkers etc.)". There is no need and it is not advisable to smell it." If this statement were true, there would be no question. However, not everyone uses prefilled containers, and the labeling is not always ideal. I'm sure this is the situation to which Dr. Bob refers...j Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Katri Tuomala Sent: Tue 5/23/2006 11:01 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Fixatives I have always read with respect Dr. Richmond's opinions, but here I have to disagree. Surely the bottle, that the specimen comes in, is labelled with what ever the fixative is, along with the pertinent patient information. It should be just as simple to say " specimen received in formalin (Bouins, Zenkers etc.)". There is no need and it is not advisable to smell it. It is an interesting mindset among many, not all, pathologist to insist in processing all tissues the same day (TAT!) ending up with inadequately fixed and consequently poorly processed tissue, which we technologist then have to struggle to produce well stained, wrinkle free, representative sections. As an immuno tech I can testify for even bigger problems in producing reliable results with immunohistochemical procedures. For purposes of immunostaining, it is important to know the time of fixation in formalin, adequate or not (24 hours is considered adequate, not 4 or even 8), so that we know what we are faced with and sometimes method can be adjusted to fit the fixation time. I would prefer doing immuno stains on a specimen, that is "overfixed" in formalin rather than underfixed. If I ever ended up with breast cancer, I would make sure, that at least one tumor section got fixed 24 hours. This is my Tuesday Rant! Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: To: Sent: Tuesday, May 23, 2006 4:22 AM Subject: [Histonet] Re: Fixatives > Patti Loykasek at PhenoPath Laboratories notes >>We are a reference lab > and > receive specimens from all over the USA. One of my "pet peeves" is that it > is > rare to see in the report exactly what type of fixative the specimen was > received in or subsequently processed in. I know we have no standard form > of > reporting, but it just seems like best practice to me to include this > information on > the report. One of my favorites is "...received in fixative..." - not very > helpful.<< > > That's exactly the phrase I use in my gross descriptions, and for a very > good > reason. I'm not about to stick my nose into every specimen bottle to > verify > that it contains formalin and not alcohol, water, or pine-scented floor > disinfectant (used at one hospital I know as a "fixative" for placentas). > I'm willing > to smell-test a very occasional container where I'm suspicious that the > wrong > fixative has been used, but not every time! > > Time of fixation is the dead horse in the middle of the living room > floor - > nobody wants to hear that the HER2 immunostain for breast cancer requires > overnight fixation, for example. > > Bob Richmond > Gastonia NC > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From dencrowl <@t> MIT.EDU Wed May 24 07:20:53 2006 From: dencrowl <@t> MIT.EDU (Denise Crowley) Date: Wed May 24 07:21:05 2006 Subject: [Histonet] cytochrome c oxidase and succinate dehydrogenase Message-ID: <5CE60D25-D94D-4942-8080-49E931647809@mit.edu> I have been asked to perform these enzyme stains on murine heart. Since this is new to me, I'm asking the experts for any favorite protocols. Even better, any commercially available kits. I also have a question about the frozen sectioning. In the clinical setting, we always froze the muscle biopsy without OCT. Is there a good reason for this? I think that filling the heart with diluted OCT will produce a nicer looking section, but I don't want to compromise the staining. Denise Crowley Facility Manager Histology Center for Cancer Research Massachusetts Institute of Technology 40 Ames St. E17-230 Cambridge MA 02139 617-258-8183 dencrowl@mit.edu From HornHV <@t> archildrens.org Wed May 24 08:36:22 2006 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed May 24 08:37:10 2006 Subject: [Histonet] Sealing Paraffin Blocks Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C63038BE0A3@EMAIL.archildrens.org> We used to do this years ago. We no longer seal blocks. In fact, I believe you cut away more on a sealed block if the block requires recutting. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kerry.l.crabb@gsk.com Sent: Tuesday, May 23, 2006 5:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sealing Paraffin Blocks What is the current opinion and practice on sealing paraffin blocks prior to filing/archiving them? What is the pro and con in your opinion? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From HornHV <@t> archildrens.org Wed May 24 08:40:38 2006 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed May 24 08:41:31 2006 Subject: [Histonet] Re: Fixatives Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C63038BE0A4@EMAIL.archildrens.org> And not all bottles say exactly what the formalin fixative is. Some labels are just a formalin warning label. Our fixative is Zinc buffered formalin. Most would probably assume the label was for 10% NBF when in fact it is not. We use ARUP Labs for a few tests we do not do and their requisition asks what fixative was used. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Wednesday, May 24, 2006 6:17 AM To: Katri Tuomala; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: Fixatives "Surely the bottle, that the specimen comes in, is labelled with what ever the fixative is, along with the pertinent patient information. It should be just as simple to say " specimen received in formalin (Bouins, Zenkers etc.)". There is no need and it is not advisable to smell it." If this statement were true, there would be no question. However, not everyone uses prefilled containers, and the labeling is not always ideal. I'm sure this is the situation to which Dr. Bob refers...j Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Katri Tuomala Sent: Tue 5/23/2006 11:01 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Re: Fixatives I have always read with respect Dr. Richmond's opinions, but here I have to disagree. Surely the bottle, that the specimen comes in, is labelled with what ever the fixative is, along with the pertinent patient information. It should be just as simple to say " specimen received in formalin (Bouins, Zenkers etc.)". There is no need and it is not advisable to smell it. It is an interesting mindset among many, not all, pathologist to insist in processing all tissues the same day (TAT!) ending up with inadequately fixed and consequently poorly processed tissue, which we technologist then have to struggle to produce well stained, wrinkle free, representative sections. As an immuno tech I can testify for even bigger problems in producing reliable results with immunohistochemical procedures. For purposes of immunostaining, it is important to know the time of fixation in formalin, adequate or not (24 hours is considered adequate, not 4 or even 8), so that we know what we are faced with and sometimes method can be adjusted to fit the fixation time. I would prefer doing immuno stains on a specimen, that is "overfixed" in formalin rather than underfixed. If I ever ended up with breast cancer, I would make sure, that at least one tumor section got fixed 24 hours. This is my Tuesday Rant! Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: To: Sent: Tuesday, May 23, 2006 4:22 AM Subject: [Histonet] Re: Fixatives > Patti Loykasek at PhenoPath Laboratories notes >>We are a reference lab > and > receive specimens from all over the USA. One of my "pet peeves" is that it > is > rare to see in the report exactly what type of fixative the specimen was > received in or subsequently processed in. I know we have no standard form > of > reporting, but it just seems like best practice to me to include this > information on > the report. One of my favorites is "...received in fixative..." - not very > helpful.<< > > That's exactly the phrase I use in my gross descriptions, and for a very > good > reason. I'm not about to stick my nose into every specimen bottle to > verify > that it contains formalin and not alcohol, water, or pine-scented floor > disinfectant (used at one hospital I know as a "fixative" for placentas). > I'm willing > to smell-test a very occasional container where I'm suspicious that the > wrong > fixative has been used, but not every time! > > Time of fixation is the dead horse in the middle of the living room > floor - > nobody wants to hear that the HER2 immunostain for breast cancer requires > overnight fixation, for example. > > Bob Richmond > Gastonia NC > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From TillRenee <@t> uams.edu Wed May 24 08:45:17 2006 From: TillRenee <@t> uams.edu (Till, Renee) Date: Wed May 24 08:45:42 2006 Subject: [Histonet] shipping tissues Message-ID: <11F927674DEBDC43B960809A7403C5D201077E76@MAILPED.ad.uams.edu> We are having some tissue samples shipped to us from another lab. They've said they can't ship in formalin, so they would fix the tissues, then ship them in a saline solution. Is this okay? It would be shipped overnight. I've never had tissue sent in. Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72002 Office (501)364-2785 Fax (501)364-3161 ================================================================================================ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ================================================================================================ From dr_masayuki2006 <@t> yahoo.co.jp Wed May 24 08:53:49 2006 From: dr_masayuki2006 <@t> yahoo.co.jp (dr_masayuki2006@yahoo.co.jp) Date: Wed May 24 08:53:58 2006 Subject: [Histonet] RNA/protein expression on myocardial biopsy sample Message-ID: <20060524135349.37898.qmail@web2409.mail.tnz.yahoo.co.jp> Are there any good products now to examine RNA/protein expression on a small sample like myocardial biopsy sample? I used Pharmagen's RPA's kit, several years ago. They had a service to perform RPA, too. I have been away from lab for several years, and now a kind of lost. Could you kindly give me any new information about handling a small amount of human sample? Which company has good chips or arrays or?? Thank you very much for your help. Masayuki MIyagishima, MD --------------------------------- Yahoo! JAPAN 10th Anniversary Special Feature --- Enjoy Yahoo! Auction with Yahoo! Mail !! --- From cgfields <@t> lexhealth.org Wed May 24 09:20:53 2006 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Wed May 24 09:16:47 2006 Subject: [Histonet] Sealing Paraffin Blocks Message-ID: They say if you seal the blocks they are good much longer for special stains, etc. We have a ring behind the block holder (from Richard-Allan) that keeps everyone's microtome lined up the same. You can also by the Histo collimator (also from RA and I think Newcomer) to line up your blocks so you will not cut the tissue away on recuts. It is a little more costly. Carole Fields, HT,ASCP Pathology Supervisor Lexington Medical Center 2720 Sunset Blvd. W. Columbia, SC 2916 -----Original Message----- From: Horn, Hazel V [mailto:HornHV@archildrens.org] Sent: Wednesday, May 24, 2006 9:36 AM To: kerry.l.crabb@gsk.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Sealing Paraffin Blocks We used to do this years ago. We no longer seal blocks. In fact, I believe you cut away more on a sealed block if the block requires recutting. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kerry.l.crabb@gsk.com Sent: Tuesday, May 23, 2006 5:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sealing Paraffin Blocks What is the current opinion and practice on sealing paraffin blocks prior to filing/archiving them? What is the pro and con in your opinion? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------------- -- The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================ == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. From MadaryJ <@t> MedImmune.com Wed May 24 09:29:19 2006 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Wed May 24 09:29:54 2006 Subject: [Histonet] Formalin on skin` Message-ID: <7CAB706201F11843BD26AD516326F0C8B00175@MD1MS007.medimmune.com> It is my understanding that formalin hardened the skin of the person who "discovered" it in pathology. After many years of exposure, bare-handed the effects on the skin were such.... I spent 16 yrs at AFIP and I remember in the 70's these bone folks who used large dishes to fix and stain would stick their entire arm in NBF and xylene. And yes the one I speak of smoked over the containers and did die of cancer. From BGapinski <@t> pathgroup.com Wed May 24 09:43:34 2006 From: BGapinski <@t> pathgroup.com (Bruce Gapinski) Date: Wed May 24 09:44:33 2006 Subject: [Histonet] RE: Histonet Digest, Vol 30, Issue 35 Message-ID: I think it's important for us all to remember that formaldehyde is a carcinogen, and that we are not (all) MDs. Therefore the anecdotal stories here could be very dangerous for someone out there, if it means they'll throw caution to the wind. Please don't do what all us "old-timers" have done. Safety has come a long way in Histology, let's all remember how important our bodies are. Peace & Thank You, Bruce Gapinski (HT) ASCP) --------------------------------------------------------------------- Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc. immediately at 615-221-4431. From awatanabe <@t> tgen.org Wed May 24 09:52:03 2006 From: awatanabe <@t> tgen.org (Aprill Watanabe) Date: Wed May 24 09:52:10 2006 Subject: [Histonet] Re: DNA/RNA Extraction Kits for paraffinised tissues In-Reply-To: <20060524134655.3D1542014980@mr1.tgen.org> Message-ID: I have used Qiagen QIAamp DNA mini kit for the last 2 years and get very good yields depending on the amount of starting material. We use routinely in our lab. I hope this helps. On 5/24/06 6:46 AM, "histonet-request@lists.utsouthwestern.edu" wrote: > DNA/RNA Extraction Kits for paraffinised tissues Aprill Watanabe, B.S. Research Associate Tissue Microarray Center (TMA) Translational Genomics Research Institute (TGen) 602-343-8822 awatanabe@tgen.org www.tgen.org From pmarcum <@t> vet.upenn.edu Wed May 24 09:59:48 2006 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Wed May 24 09:59:58 2006 Subject: [Histonet] Formalin on skin` In-Reply-To: <7CAB706201F11843BD26AD516326F0C8B00175@MD1MS007.medimmune. com> References: <7CAB706201F11843BD26AD516326F0C8B00175@MD1MS007.medimmune.com> Message-ID: <6.1.1.1.2.20060524104215.019a4ed8@mail.vet.upenn.edu> At 10:29 AM 5/24/2006, Madary, Joseph wrote: >It is my understanding that formalin hardened the skin of the person who >"discovered" it in pathology. After many years of exposure, bare-handed >the effects on the skin were such.... > >I spent 16 yrs at AFIP and I remember in the 70's these bone folks who >used large dishes to fix and stain would stick their entire arm in NBF >and xylene. And yes the one I speak of smoked over the containers and >did die of cancer. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Unfortunately when some of us started in histology in 60s and 70s some of the pathologists were not aware of issues either. My first pathologist advised that if one had paraffin all over your hands the solution was to clean them with xylenes. Later I had a department chair who had his lab (in the late 70s) using amyl acetate to clear brain tissue and following with benzene. As if this was not enough the lab used Paraplast Plus with DMSO in a 50/50 mix for the first infiltration steps. The DMSO just helped get more fumes into the lungs and mucosal linings with the old style open tissue processor. His histologists all had contact dermatitis and constant coughing. Our lab refused to use this procedure and developed a method for whole brains using butanol as the clearing agent. It was not only safer (in the scheme of things) we lost very few brains due to over hardening or sections as it was gentler and easier to use. So we have all those stories we all lived through and now can only hope the exposures are or won't kill us soon. I know when I lecture and talk about these things younger people can't believe it happened. We have the right to know now and unfortunately not everyone takes advantage and reads the MSDSs sheets that come with the products they use daily. We did not know back then because no one was looking at safety the way we do now. Today there is not excuse not to know between the companies and the Internet. I know people who feel after all the exposure they have had why worry now. The last line of you reply says it all. It is never to late to be safe. PS I still can't coverslip with gloves on and I have tried for years. Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From liz <@t> premierlab.com Wed May 24 10:04:05 2006 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed May 24 10:04:19 2006 Subject: [Histonet] shipping tissues In-Reply-To: <11F927674DEBDC43B960809A7403C5D201077E76@MAILPED.ad.uams.edu> Message-ID: <001b01c67f43$4d35b6a0$0300a8c0@Chlipala> Renee We do this all of the time, the samples turn out just fine. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Till, Renee Sent: Wednesday, May 24, 2006 7:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] shipping tissues We are having some tissue samples shipped to us from another lab. They've said they can't ship in formalin, so they would fix the tissues, then ship them in a saline solution. Is this okay? It would be shipped overnight. I've never had tissue sent in. Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72002 Office (501)364-2785 Fax (501)364-3161 ======================================================================== ======================== Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ======================================================================== ======================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1.1555 (20060524) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From Jackie.O'Connor <@t> abbott.com Wed May 24 10:23:24 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed May 24 10:23:54 2006 Subject: [Histonet] shipping tissues In-Reply-To: <11F927674DEBDC43B960809A7403C5D201077E76@MAILPED.ad.uams.edu> Message-ID: Yes, they CAN ship in formalin (how do they get their formalin - it's shipped). It just cannot go by air because of the hazardous nature. It has to go via ground transportation. I wouldn't advise a transfer to saline - JO'C "Till, Renee" Sent by: histonet-bounces@lists.utsouthwestern.edu 05/24/2006 08:45 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] shipping tissues We are having some tissue samples shipped to us from another lab. They've said they can't ship in formalin, so they would fix the tissues, then ship them in a saline solution. Is this okay? It would be shipped overnight. I've never had tissue sent in. Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72002 Office (501)364-2785 Fax (501)364-3161 ================================================================================================ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ================================================================================================ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dsnider <@t> shrinenet.org Wed May 24 10:24:18 2006 From: dsnider <@t> shrinenet.org (Snider, Deanna) Date: Wed May 24 10:24:28 2006 Subject: [Histonet] embedding centers Message-ID: <84BE46B37B314D409C5A17B7BAB022D68BC9CF@IDC-EX-VS01.shriners.cc> Hello all! I need your masses of expertise yet again!! I thank you in advance. My lab is in the process of acquiring a new embedding center. What are your experiences with the various embedding centers out there? Why do you like/dislike about the unit? How is your experience with the customer support? I am considering the Microm ec350, so any info on this particular unit is greatly appreciated. Thanks so much again! Deanna Snider HT ASCP Lead Histotechnician, Research Department Shriners Hospital for Children Cincinnati, OH 45229 513-872-6388 CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients. If you are not the intended recipient, (or authorized to receive for the recipient) you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please destroy all copies of this communication and any attachments and contact the sender by reply e-mail or telephone (813) 281-0300. From Barry.R.Rittman <@t> uth.tmc.edu Wed May 24 10:32:00 2006 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Wed May 24 10:32:06 2006 Subject: [Histonet] Sealing Paraffin Blocks Message-ID: I am a strong believer in sealing blocks. While paraffin does penetrate into cells etc. it is still possible for water (and organisms of various types) to penetrate into the block. After all we use water to soak into some blocks to enhance cutting. I have seen stored, unsealed blocks, with massive penetration of fungi throughout the entire tissue. Also if unsealed blocks are stored in a dry atmosphere they will tend to shrink. If you are going to store blocks for any length of time I would seal them not just dipping them into molten paraffin but melting the surface layer. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carole Fields Sent: Wednesday, May 24, 2006 9:21 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Sealing Paraffin Blocks They say if you seal the blocks they are good much longer for special stains, etc. We have a ring behind the block holder (from Richard-Allan) that keeps everyone's microtome lined up the same. You can also by the Histo collimator (also from RA and I think Newcomer) to line up your blocks so you will not cut the tissue away on recuts. It is a little more costly. Carole Fields, HT,ASCP Pathology Supervisor Lexington Medical Center 2720 Sunset Blvd. W. Columbia, SC 2916 -----Original Message----- From: Horn, Hazel V [mailto:HornHV@archildrens.org] Sent: Wednesday, May 24, 2006 9:36 AM To: kerry.l.crabb@gsk.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Sealing Paraffin Blocks We used to do this years ago. We no longer seal blocks. In fact, I believe you cut away more on a sealed block if the block requires recutting. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kerry.l.crabb@gsk.com Sent: Tuesday, May 23, 2006 5:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sealing Paraffin Blocks What is the current opinion and practice on sealing paraffin blocks prior to filing/archiving them? What is the pro and con in your opinion? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ ---- -- The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ======================================================================== ==== == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NSEARCY <@t> swmail.sw.org Wed May 24 10:41:41 2006 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Wed May 24 10:42:05 2006 Subject: [Histonet] Pathologist Assistant Message-ID: I have had a request from a pathologist to attempt getting our grossing assistant qualified to take the PA exam. This person just completed the degree; has been grossing ~ 1. 5 years after training , and being deemed competent on "simple" cases, bx's, tonsils, etc.( by the certified PA & pathologist) He has/ is performing histology, autopsies, etc. Both the certified PA and myself (which obviously is not good enough) has explained that he is NOT qualified under the new ASCP guidelines but I have to do it and secure documentation. Can someone point me in the right direction to secure what I need? Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 254-724-2438 -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Nita Searcy TEL;WORK:4-2438 ORG:;Anatomic Pathology EMAIL;WORK;PREF;NGW:NSEARCY@swmail.sw.org N:Searcy;Nita TITLE:Manager, Pathology Division END:VCARD From Jackie.O'Connor <@t> abbott.com Wed May 24 10:59:47 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed May 24 11:00:13 2006 Subject: [Histonet] Sealing Paraffin Blocks In-Reply-To: Message-ID: Sealing also deters rodents and roaches from eating the blocks - I've seen this in the warmer parts of the US - I've worked at some pretty old places. "Rittman, Barry R" Sent by: histonet-bounces@lists.utsouthwestern.edu 05/24/2006 10:32 AM To cc Subject RE: [Histonet] Sealing Paraffin Blocks I am a strong believer in sealing blocks. While paraffin does penetrate into cells etc. it is still possible for water (and organisms of various types) to penetrate into the block. After all we use water to soak into some blocks to enhance cutting. I have seen stored, unsealed blocks, with massive penetration of fungi throughout the entire tissue. Also if unsealed blocks are stored in a dry atmosphere they will tend to shrink. If you are going to store blocks for any length of time I would seal them not just dipping them into molten paraffin but melting the surface layer. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carole Fields Sent: Wednesday, May 24, 2006 9:21 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Sealing Paraffin Blocks They say if you seal the blocks they are good much longer for special stains, etc. We have a ring behind the block holder (from Richard-Allan) that keeps everyone's microtome lined up the same. You can also by the Histo collimator (also from RA and I think Newcomer) to line up your blocks so you will not cut the tissue away on recuts. It is a little more costly. Carole Fields, HT,ASCP Pathology Supervisor Lexington Medical Center 2720 Sunset Blvd. W. Columbia, SC 2916 -----Original Message----- From: Horn, Hazel V [mailto:HornHV@archildrens.org] Sent: Wednesday, May 24, 2006 9:36 AM To: kerry.l.crabb@gsk.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Sealing Paraffin Blocks We used to do this years ago. We no longer seal blocks. In fact, I believe you cut away more on a sealed block if the block requires recutting. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kerry.l.crabb@gsk.com Sent: Tuesday, May 23, 2006 5:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sealing Paraffin Blocks What is the current opinion and practice on sealing paraffin blocks prior to filing/archiving them? What is the pro and con in your opinion? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ ---- -- The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ======================================================================== ==== == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charles.Embrey <@t> carle.com Wed May 24 11:18:17 2006 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Wed May 24 11:29:11 2006 Subject: [Histonet] Pathologist Assistant Message-ID: This is from the ASCP BOR site: https://www.ascp.org/Certification/pdf/booklet.pdf The OJT route is closing soon so if he hasn't graduated from an accredited PA school he had better hurry. The ONLY test given now for PA certification is the ASCP/BOR exam. The AAPA has discontinued the Fellowship exam. PA(ASCP) Pathologists' Assistant To be eligible for this examination category, an applicant must satisfy the requirements of at least one of the following routes: Route 1: Baccalaureate degree from a regionally accredited college/university AND successful completion of a NAACLS accredited Pathologists' Assistant program within the last five years, OR Route 2: Baccalaureate degree from a regionally accredited college/university with 20 semester hours (30 quarter hours) of biology, AND three years full time acceptable experience as a Pathologists' Assistant within the last ten years. The three years of experience must be under the supervision of a pathologist (certified by the American Board of Pathology in Anatomic Pathology). Route 3: Baccalaureate degree from a regionally accredited college/university with a combination of 24 semester hours (36 quarter hours) of biology and chemistry, AND five years of full time acceptable experience in hemapheresis within the last ten years under the direction of a Medical Director, OR Route 4: Doctorate in Medicine with current state or provincial license AND one year of full time acceptable experience in hemapheresis within the last ten years under the direction of a Medical Director. NOTE: Applicants applying under Routes 1 and 4 must submit a notarized copy of their current state or provincial license. A SCP BOARD OF REGI S TRY Clinical Laboratory Experience To fulfill the experience requirement for the Pathologists' Assistant examination, you must have experience, within the last ten years, in the following areas: * Preparation, gross description, and dissection of human tissue surgical specimens, including a minimum of 20 different specimens from the following list: Skin excision; Parathyroidectomy; Thyroidectomy, complete or partial; Breast lumpectomy; Mastectomy for tumor; Fetus and placenta; Placenta; Products of conception or ectopic pregnancy; Liver or kidney biopsy; Appendectomy; Lymphoma work-up; Colectomy/enterectomy for tumor; Colectomy/enterectomy for non-tumor; Tonsillectomy; Splenectomy; Brain biopsy or lobectomy; Nerve or muscle biopsy; Bone, resection or excision; Bone biopsy, cyst or tumor; Femoral head; Lung, lobectomy or pneumonectomy; Amputation; Nephrectomy, complete or partial; Choleocystectomy; Radical neck or laryngectomy specimen; Hepatectomy, transplant or lobectomy; Radical prostatectomy; Cystectomy (urinary bladder); Gastrectomy; Oophorectomy; Soft tissue mass: lipoma, hemangioma, lymphangioma, sarcoma, etc.; Hysterectomy Orchiectomy; Adrenalectomy * Obtaining biological specimens such as blood, tissue and toxicological material for analysis * Photographing gross specimens and microscopic slides as directed * Preparation and prosection of a minimum of 10 human postmortem examinations. These must include: a. At least 8 adult or pediatric complete autopsies b. At least 2 of the autopsies must include brain c. May use 2 fetal autopsies (20 weeks or 500 grams/ not surgical specimen) d. Must have name or initials on report or be mentioned in narrative e. PAD, Clinical Summary & FAD * Selecting, preparing and submitting appropriate gross tissue sections for frozen section Good Luck and e-mail me if I can be of any further assistance: Charles Embrey PA(ASCP) www.greyrealm.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Wednesday, May 24, 2006 10:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathologist Assistant I have had a request from a pathologist to attempt getting our grossing assistant qualified to take the PA exam. This person just completed the degree; has been grossing ~ 1. 5 years after training , and being deemed competent on "simple" cases, bx's, tonsils, etc.( by the certified PA & pathologist) He has/ is performing histology, autopsies, etc. Both the certified PA and myself (which obviously is not good enough) has explained that he is NOT qualified under the new ASCP guidelines but I have to do it and secure documentation. Can someone point me in the right direction to secure what I need? Thanks Nita Searcy, HT/HTL (ASCP) Scott and White Hospital Division Manager, Anatomic Pathology 2401 S. 31st. Street 254-724-2438 Temple, Texas, 76502 nsearcy@swmail.sw.org 254-724-2438 254-724-2438 From cgfields <@t> lexhealth.org Wed May 24 11:44:13 2006 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Wed May 24 11:40:10 2006 Subject: FW: [Histonet] Sealing Paraffin Blocks Message-ID: -----Original Message----- From: Carole Fields Sent: Wednesday, May 24, 2006 12:43 PM To: 'Rittman, Barry R' Subject: RE: [Histonet] Sealing Paraffin Blocks You can also run the block across the hot plate on the embedding center and it seals them really quickly. Once you get the hang of it it is a fast process. I agree about the bugs and mice....yuk. Carole Fields -----Original Message----- From: Rittman, Barry R [mailto:Barry.R.Rittman@uth.tmc.edu] Sent: Wednesday, May 24, 2006 11:32 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Sealing Paraffin Blocks I am a strong believer in sealing blocks. While paraffin does penetrate into cells etc. it is still possible for water (and organisms of various types) to penetrate into the block. After all we use water to soak into some blocks to enhance cutting. I have seen stored, unsealed blocks, with massive penetration of fungi throughout the entire tissue. Also if unsealed blocks are stored in a dry atmosphere they will tend to shrink. If you are going to store blocks for any length of time I would seal them not just dipping them into molten paraffin but melting the surface layer. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carole Fields Sent: Wednesday, May 24, 2006 9:21 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Sealing Paraffin Blocks They say if you seal the blocks they are good much longer for special stains, etc. We have a ring behind the block holder (from Richard-Allan) that keeps everyone's microtome lined up the same. You can also by the Histo collimator (also from RA and I think Newcomer) to line up your blocks so you will not cut the tissue away on recuts. It is a little more costly. Carole Fields, HT,ASCP Pathology Supervisor Lexington Medical Center 2720 Sunset Blvd. W. Columbia, SC 2916 -----Original Message----- From: Horn, Hazel V [mailto:HornHV@archildrens.org] Sent: Wednesday, May 24, 2006 9:36 AM To: kerry.l.crabb@gsk.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Sealing Paraffin Blocks We used to do this years ago. We no longer seal blocks. In fact, I believe you cut away more on a sealed block if the block requires recutting. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of kerry.l.crabb@gsk.com Sent: Tuesday, May 23, 2006 5:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sealing Paraffin Blocks What is the current opinion and practice on sealing paraffin blocks prior to filing/archiving them? What is the pro and con in your opinion? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ ---- -- The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ======================================================================== ==== == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. From nyilmaz <@t> mersin.edu.tr Wed May 24 11:52:58 2006 From: nyilmaz <@t> mersin.edu.tr (nyilmaz@mersin.edu.tr) Date: Wed May 24 11:53:27 2006 Subject: [Histonet] Sterotaxic instrument drawings Message-ID: <20060524165258.0CB5A48085@mail.mersin.edu.tr> Dear colleagues... We're plannig to build a sterotaxic instrument for animal brain studies. It must be adaptable for mouse, rat and rabbit. We're looking for plans, drawings etc. anything beneficial. Does anybody know this kind of materials reachable on internet or another source? Thanks in advance... Dr. Necat Yilmaz Mersin University School of Medicine Histology and Embryology Dept. Mersin/TURKEY From safety.trainorlab <@t> mts.net Wed May 24 11:56:52 2006 From: safety.trainorlab <@t> mts.net (SAFETY TRAINORLAB) Date: Wed May 24 11:56:55 2006 Subject: [Histonet] Re: Histonet Digest, Vol 30, Issue 35, re: formalin exposure References: <4vbmbg$c0lbrt@wnpgmb02-c600b.mts.net> Message-ID: <000501c67f53$0e37f840$64c809c0@tlabs> Hi, Formaldehyde is a carcinogen and is list a one of the top ten toxic chemicals used in industry. It is proven to be a carcinogen in humans mainly GI and liver. It is also an Immuno, Neuro, Reproductive and Respiratory toxin. Not to mention a skin sensitizer. Anyone using formaldehyde should minimize there exposure to it and if possible eliminate it completely. At our lab, we will be switching to a formalin substitute. Adam ----- Original Message ----- From: To: Sent: Wednesday, May 24, 2006 8:47 AM Subject: Histonet Digest, Vol 30, Issue 35 > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Formalin on skin (Gudrun Lang) > 2. (no subject) (Paul Cremers) > 3. RE: Formalin on skin (Charles.Embrey) > 4. Shirley on leave : 24 May 2006 (Wednesday) (Shirley PHUA) > 5. Re: Formalin on skin (Bryan Llewellyn) > 6. Re: Formalin on skin (Chris Pomajzl) > 7. re:Hoechst staining... (Carl Hobbs) > 8. Re: Formalin on skin (Jackie M O'Connor) > 9. Fw: Source of Re: [Histonet] Incubation chamber (Jan Shivers) > 10. GSH meeting (Shirley Powell) > 11. Re: STAFF EVALUATIONS (Patti Loykasek) > 12. Sealing Paraffin Blocks (kerry.l.crabb@gsk.com) > 13. Re: Re: Fixatives (Patti Loykasek) > 14. RE: DNA/RNA Extraction Kits for paraffinised tissues > (Makhijani, Nalini S) > 15. Re: tyrosine hydroxylase staining?? (Adam Perry) > 16. Re: Re: Fixatives (Katri Tuomala) > 17. RE: Formalin on skin (Kemlo Rogerson) > 18. RE: Formalin on skin (Kemlo Rogerson) > 19. RE: Formalin on skin (Kemlo Rogerson) > 20. RE: Hoechst staining method (Melanie Black) > 21. RE: Re: Fixatives (Weems, Joyce) > 22. cytochrome c oxidase and succinate dehydrogenase (Denise Crowley) > 23. RE: Sealing Paraffin Blocks (Horn, Hazel V) > 24. RE: Re: Fixatives (Horn, Hazel V) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 23 May 2006 19:32:18 +0200 > From: "Gudrun Lang" > Subject: [Histonet] Formalin on skin > To: "Histonetliste \(Histonetliste\)" > > Message-ID: <000101c67e8e$d7693550$eeeea8c0@SERVER01> > Content-Type: text/plain; charset="us-ascii" > > Hi, > Today a labassistent asked me, what would happen to her skin, if formalin > swallowed over her arm. > I said, the formalin would harden the skin, but only on the surface. She > should wash the arm with plenty of water, and there will reamain no negative > reaction. It would not be dangerous for her health. > > Do you agree with me? Is there a cancer-risk after short contact with > formalin? > > Greetings > Gudrun Lang > > Histolab > Akh Linz > Austria > > > > > ------------------------------ > > Message: 2 > Date: Tue, 23 May 2006 13:35:58 -0400 > From: "Paul Cremers" > Subject: [Histonet] (no subject) > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; format="flowed" > > > Hello to all, > > Is there anyone looking for a histotech or two. My wife and I > are wanting to move to the Boulder or Fort Collins, Colorado area. We > are also interested in Portland, Salem, Corvalis and Eugene, Oregon. > We are both HT certified and are wanting to start somewhere between > mid-August and September 1. > > Thanks, > > Paul and Melissa Cremers > > > ------------------------------ > > Message: 3 > Date: Tue, 23 May 2006 12:56:15 -0500 > From: "Charles.Embrey" > Subject: RE: [Histonet] Formalin on skin > To: , "Histonetliste \(Histonetliste\)" > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > You are 100% correct. I have even reached a bare hand into formalin to > pull out something before but washed with soap and water minutes after. > I have not personally heard of formalin causing an increased risk of > skin cancer however I am sure that there is a study somewhere that has > probably associated some risk with L---O---N---G term exposure. Your > labassistant should be perfectly safe. Just don't drink it and keep it > out of the eyes :) > > Charles Embrey Jr. PA(ASCP) > www.greyrealm.com > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun > Lang > Sent: Tuesday, May 23, 2006 12:32 PM > To: Histonetliste (Histonetliste) > Subject: [Histonet] Formalin on skin > > Hi, > Today a labassistent asked me, what would happen to her skin, if > formalin > swallowed over her arm. > I said, the formalin would harden the skin, but only on the surface. She > should wash the arm with plenty of water, and there will reamain no > negative > reaction. It would not be dangerous for her health. > > Do you agree with me? Is there a cancer-risk after short contact with > formalin? > > Greetings > Gudrun Lang > > Histolab > Akh Linz > Austria > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 4 > Date: Wed, 24 May 2006 02:11:23 +0800 > From: Shirley PHUA > Subject: [Histonet] Shirley on leave : 24 May 2006 (Wednesday) > To: histonet > Message-ID: > > > Content-Type: text/plain; charset=US-ASCII > > I will be out of the office from 24/05/2006 to 24/05/2006. > > I am away on 1 day leave. > > I will return on 25/05/2006 (Thursday). > > Pathologists : I will process your requests when I return. Otherwise, if > urgent, please contact Henry. > > > > > ------------------------------ > > Message: 5 > Date: Tue, 23 May 2006 12:12:39 -0700 > From: Bryan Llewellyn > Subject: Re: [Histonet] Formalin on skin > To: Histonet > Message-ID: <001201c67e9c$dbefc680$130e4246@yourlk4rlmsu> > Content-Type: text/plain; format=flowed; charset=iso-8859-1; > reply-type=original > > Most of us old-timers have stuck our fingers in formalin or spilled it on > ourselves on many occasions. I have never heard of anyone developing a > tumour from it, though. It certainly doesn't appear to be common if it does > happen. In Canada some years ago we had a government program assisting > people to inject urea-formaldehyde resin into the walls of houses as a > retrofit insulation system. A few years later it was decided the formalin > fumes given off were carcinogenic, so we had another program to remove it. > That's about the only link to tumours I have heard of. > > One of the pathologists I used to work with many years ago was fond of > quoting a paper he had once read which compared naso-pharyngeal tumour rates > among various groups. Pathologists had a lower rate than other people. He > always put that down to the formalin fumes they breathed in all the time. > > The real identified problem with formalin and skin contact is dermatitis. > It isn't universal, but some people do get it with repeated contact, and > once you develop the sensitivity to it, it doesn't go away. So wear gloves. > > Bryan Llewellyn > > > > ----- Original Message ----- > From: "Charles.Embrey" > To: ; "Histonetliste (Histonetliste)" > > Sent: Tuesday, May 23, 2006 10:56 AM > Subject: RE: [Histonet] Formalin on skin > > > You are 100% correct. I have even reached a bare hand into formalin to > pull out something before but washed with soap and water minutes after. > I have not personally heard of formalin causing an increased risk of > skin cancer however I am sure that there is a study somewhere that has > probably associated some risk with L---O---N---G term exposure. Your > labassistant should be perfectly safe. Just don't drink it and keep it > out of the eyes :) > > Charles Embrey Jr. PA(ASCP) > www.greyrealm.com > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun > Lang > Sent: Tuesday, May 23, 2006 12:32 PM > To: Histonetliste (Histonetliste) > Subject: [Histonet] Formalin on skin > > Hi, > Today a labassistent asked me, what would happen to her skin, if > formalin > swallowed over her arm. > I said, the formalin would harden the skin, but only on the surface. She > should wash the arm with plenty of water, and there will reamain no > negative > reaction. It would not be dangerous for her health. > > Do you agree with me? Is there a cancer-risk after short contact with > formalin? > > Greetings > Gudrun Lang > > Histolab > Akh Linz > Austria > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 6 > Date: Tue, 23 May 2006 13:51:49 -0500 > From: "Chris Pomajzl" > Subject: Re: [Histonet] Formalin on skin > To: "HISTONET" > Message-ID: <004301c67e99$f31fa030$26fca8c0@CSP> > Content-Type: text/plain; charset="iso-8859-1" > > I would imagine that if someone had open wounds or breaks in the skin, there > could be acute problems. But chronic long-term problems are unlikely with > such a brief incident. > > People drink formaldehyde (or at least used to, wasn't it a preservative in > beer many years ago??), they smoke it (I've heard of people dipping joints > in formaldehyde for a stronger buzz, never tried it myself), and we breathe > it in the lab every day. I believe that the carcinogenic effects are due to > long-term exposure in laboratory animals. I am not aware of any studies > involving human subjects. > > Hope this helps. > > ----- Original Message ----- > From: "Charles.Embrey" > To: ; "Histonetliste (Histonetliste)" > > Sent: Tuesday, May 23, 2006 12:56 PM > Subject: RE: [Histonet] Formalin on skin > > > You are 100% correct. I have even reached a bare hand into formalin to > pull out something before but washed with soap and water minutes after. > I have not personally heard of formalin causing an increased risk of > skin cancer however I am sure that there is a study somewhere that has > probably associated some risk with L---O---N---G term exposure. Your > labassistant should be perfectly safe. Just don't drink it and keep it > out of the eyes :) > > Charles Embrey Jr. PA(ASCP) > www.greyrealm.com > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun > Lang > Sent: Tuesday, May 23, 2006 12:32 PM > To: Histonetliste (Histonetliste) > Subject: [Histonet] Formalin on skin > > Hi, > Today a labassistent asked me, what would happen to her skin, if > formalin > swallowed over her arm. > I said, the formalin would harden the skin, but only on the surface. She > should wash the arm with plenty of water, and there will reamain no > negative > reaction. It would not be dangerous for her health. > > Do you agree with me? Is there a cancer-risk after short contact with > formalin? > > Greetings > Gudrun Lang > > Histolab > Akh Linz > Austria > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 7 > Date: Tue, 23 May 2006 21:30:39 +0100 > From: "Carl Hobbs" > Subject: [Histonet] re:Hoechst staining... > To: "Histonet" > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Of nuclei! > Easy.....If you wish, check out this site for a protocol and images > http://www.immunoportal.com/index.php > Saves me fingers retyping ;-) > It's a standard, tho'. No fancy stuff reqd. Lots of XPerienced people here > to help, that I will always pay greatest respects to. > Regards > > > > > ------------------------------ > > Message: 8 > Date: Tue, 23 May 2006 15:32:56 -0500 > From: "Jackie M O'Connor" > Subject: Re: [Histonet] Formalin on skin > To: "Chris Pomajzl" > Cc: HISTONET > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > Formaldehyde was a common ingredient of shampoos, and I remember reading > the ingredients (with horror) on a bottle of Mr. Bubble bubble bath for > kids (like 25 years ago). If you remember "Good Morning Vietnam" - they > mentioned using formaldehyde to produce a better head on a glass of beer > from the tap. > I once worked with a pathologist who was allergic to formaldehyde. When > he did the gross, we had to rinse all the specimens in H20 before he could > examine them - yeah, that was fun. > Jackie O' > > > > > "Chris Pomajzl" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 05/23/2006 01:51 PM > > To > "HISTONET" > cc > > Subject > Re: [Histonet] Formalin on skin > > > > > > > I would imagine that if someone had open wounds or breaks in the skin, > there > could be acute problems. But chronic long-term problems are unlikely with > such a brief incident. > > People drink formaldehyde (or at least used to, wasn't it a preservative > in > beer many years ago??), they smoke it (I've heard of people dipping joints > in formaldehyde for a stronger buzz, never tried it myself), and we > breathe > it in the lab every day. I believe that the carcinogenic effects are due > to > long-term exposure in laboratory animals. I am not aware of any studies > involving human subjects. > > Hope this helps. > > ----- Original Message ----- > From: "Charles.Embrey" > To: ; "Histonetliste (Histonetliste)" > > Sent: Tuesday, May 23, 2006 12:56 PM > Subject: RE: [Histonet] Formalin on skin > > > You are 100% correct. I have even reached a bare hand into formalin to > pull out something before but washed with soap and water minutes after. > I have not personally heard of formalin causing an increased risk of > skin cancer however I am sure that there is a study somewhere that has > probably associated some risk with L---O---N---G term exposure. Your > labassistant should be perfectly safe. Just don't drink it and keep it > out of the eyes :) > > Charles Embrey Jr. PA(ASCP) > www.greyrealm.com > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun > Lang > Sent: Tuesday, May 23, 2006 12:32 PM > To: Histonetliste (Histonetliste) > Subject: [Histonet] Formalin on skin > > Hi, > Today a labassistent asked me, what would happen to her skin, if > formalin > swallowed over her arm. > I said, the formalin would harden the skin, but only on the surface. She > should wash the arm with plenty of water, and there will reamain no > negative > reaction. It would not be dangerous for her health. > > Do you agree with me? Is there a cancer-risk after short contact with > formalin? > > Greetings > Gudrun Lang > > Histolab > Akh Linz > Austria > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 9 > Date: Tue, 23 May 2006 16:55:27 -0500 > From: "Jan Shivers" > Subject: Fw: Source of Re: [Histonet] Incubation chamber > To: "histonet" > Message-ID: <012801c67eb3$9a32e3f0$a1065486@auxs.umn.edu> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=response > > Years ago when my IHC lab was a fledgling entity with very little budget, I > used a 9x13" metal cake pan (with lid), wet paper towels for humidity in the > bottom, and disposable 1 ml pipets as the slide racks. They held two rows > of 10 slides each. Just had to make sure my wet paper towels laid flat (no > bubbles), otherwise they'd make the pipets sit uneven. > > Jan Shivers > UMN VDL > > ----- Original Message ----- > From: "Gayle Callis" > To: "Geoff McAuliffe" ; > > Sent: Tuesday, May 23, 2006 10:21 AM > Subject: Source of Re: [Histonet] Incubation chamber > > > > Evergreen Scientific has a 10 slide chamber, plastic, with lid. The > > chamber is designed so slides are elevated, just add water to bottom of > > wells for humidity. There are 3 chambers per package, very inexpensive. > > You can stack them. They come either black plastic for fluorescence work > > OR clear. I have seen them sold other places to, maybe EMS is the place. > > Evergreen makes them. > > > > Very tidy little devices for low volume work > > > > At 08:06 AM 5/23/2006, you wrote: > >>I use "Tupperware", or a generic equivalent. Slides are supported by glass > >>rods in the bottom of the container. Add a little water and seal. > >> > >>Geoff > > > > Gayle Callis > > MT,HT,HTL(ASCP) > > Research Histopathology Supervisor > > Veterinary Molecular Biology > > Montana State University - Bozeman > > PO Box 173610 > > Bozeman MT 59717-3610 > > 406 994-6367 > > 406 994-4303 (FAX) > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > ------------------------------ > > Message: 10 > Date: Tue, 23 May 2006 18:00:28 -0400 > From: Shirley Powell > Subject: [Histonet] GSH meeting > To: histonet@lists.utsouthwestern.edu > Message-ID: <01M2RQRHLNHW8WWEFM@Macon2.Mercer.edu> > Content-Type: text/plain; charset=US-ASCII > > Reminder:::: > > > The Georgia Society for Histotechnology and the Georgia Society of Cytology > will hold their 4th Annual Combined Scientific Symposium on Saturday, June > 3, 2006, in Augusta, Georgia at the Marriott Augusta Hotel and Suites, 2 > Tenth Street, Augusta, GA 30901. The phone number for reservations is 1-706 > 722-8900. > > I will email you a program upon request. I also have vendor information for > any who wish to exhibit. > > Thanks, > > Shirley Powell > GSH Secretary/Membership Chair > > > > > ------------------------------ > > Message: 11 > Date: Tue, 23 May 2006 15:39:28 -0700 > From: Patti Loykasek > Subject: Re: [Histonet] STAFF EVALUATIONS > To: Diana McCaig , histonet > > Message-ID: > Content-Type: text/plain; charset="US-ASCII" > > Hi Diana. I would be interested in your sharing the info that you get back. > I can tell you what we do. I'll start off by saying we are constantly > updating/revising our program. I feel like we do a better job each year. > Some tasks are evaluated by observation & documented on a spreadsheet that > contains all SOPs (by name) that a particular staff should know. I do have > checklists for equipment that cover things from do staff know where on/off > is, to the various functions of the equipment staff should know. For the > histologists we evaluate H&E sections (both paraffin & FS) with criteria > similar to the board registry & include some safety & clean up details. I'm > putting together a test to cover knowledge of general IHC & would like to > get some photos of common stains acceptable & not for a test. A work in > progress - always. > Hope that helps a bit. > > > Patti Loykasek BS, HTL, QIHC > PhenoPath Laboratories > Seattle, WA > > > > > > Does anyone do an annual evaluation of staff ability to perform certain tasks? > > Diana McCaig 519-352-6401 (6604) > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------------------------------------------------- > This e-mail message, including any attachments, is for the sole use of > the intended recipients and may contain privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If > you are not the intended recipient, please contact the sender by e-mail > and destroy all copies of the original message, or you may call PhenoPath > Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > > > > > ------------------------------ > > Message: 12 > Date: Tue, 23 May 2006 18:45:19 -0400 > From: kerry.l.crabb@gsk.com > Subject: [Histonet] Sealing Paraffin Blocks > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=us-ascii > > What is the current opinion and practice on sealing paraffin blocks prior > to filing/archiving them? What is the pro and con in your opinion? > > ------------------------------ > > Message: 13 > Date: Tue, 23 May 2006 15:46:19 -0700 > From: Patti Loykasek > Subject: Re: [Histonet] Re: Fixatives > To: > Message-ID: > Content-Type: text/plain; charset="US-ASCII" > > Dr. Richmond, I always appreciate your views and comments. I do think if a > bottle comes to the lab with a label noting fixative it could be dictated as > "...received in container labeled ..." or ",,,received in unlabeled > container..", this is what was done at previous facilities I have worked, > but perhaps is not the norm. > I do agree that the elephant in the room is how poor or improper > fixation/processing affects everything downstream. I could go on & on about > this issue. I feel that CAP nor anyone else does very little to address this > issue. Everyone turns a bit of a blind eye to it. > Thanks for the input. > > > Patti Loykasek BS, HTL, QIHC > PhenoPath Laboratories > Seattle, WA > > > > > > > Patti Loykasek at PhenoPath Laboratories notes >>We are a reference lab and > > receive specimens from all over the USA. One of my "pet peeves" is that it is > > rare to see in the report exactly what type of fixative the specimen was > > received in or subsequently processed in. I know we have no standard form of > > reporting, but it just seems like best practice to me to include this > > information on > > the report. One of my favorites is "...received in fixative..." - not very > > helpful.<< > > > > That's exactly the phrase I use in my gross descriptions, and for a very good > > reason. I'm not about to stick my nose into every specimen bottle to verify > > that it contains formalin and not alcohol, water, or pine-scented floor > > disinfectant (used at one hospital I know as a "fixative" for placentas). I'm > > willing > > to smell-test a very occasional container where I'm suspicious that the wrong > > fixative has been used, but not every time! > > > > Time of fixation is the dead horse in the middle of the living room floor - > > nobody wants to hear that the HER2 immunostain for breast cancer requires > > overnight fixation, for example. > > > > Bob Richmond > > Gastonia NC > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------------------------------------------------- > This e-mail message, including any attachments, is for the sole use of > the intended recipients and may contain privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If > you are not the intended recipient, please contact the sender by e-mail > and destroy all copies of the original message, or you may call PhenoPath > Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > > > > > ------------------------------ > > Message: 14 > Date: Tue, 23 May 2006 17:09:02 -0700 > From: "Makhijani, Nalini S" > Subject: RE: [Histonet] DNA/RNA Extraction Kits for paraffinised > tissues > To: "Shirley PHUA" , > > Message-ID: > <715FA772CEA81A47B1A762AB6E45123A0111B549@VHAV22MSGA3.v22.med.va.gov> > Content-Type: text/plain; charset="US-ASCII" > > Ambion advertises a kit, "RecoverAll", for total RNA and DNA extraction > from FFPE tissues. I've never used it, but you could get more > information from their web site: www.ambion.com/prod/recoverall. > Hope this helps! > > Nalini Makhijani, M.S. > Research Biologist > VA Greater Los Angeles Healthcare System > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley > PHUA > Sent: Monday, May 22, 2006 7:32 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] DNA/RNA Extraction Kits for paraffinised tissues > > Dear All > > Have anyone heard of any DNA/RNA extraction kits for formalin-fixed, > paraffinised tissues? I will greatly appreciate if you can share some of > > your feedbacks. Were your yields good? > > Thanks in advance! > > Shirley Phua > Histopathology Laboratory > Centre for Forensic Medicine > Health Sciences Authority > DID : 62130654 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 15 > Date: Tue, 23 May 2006 17:17:35 -0700 (PDT) > From: Adam Perry > Subject: Re: [Histonet] tyrosine hydroxylase staining?? > To: histonet@lists.utsouthwestern.edu > Message-ID: <20060524001735.22829.qmail@web30413.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hey Johanna, > > Just curious...when you say you get weak, diffuse staining all over the brain, is that in sections that should contain dopaminergic cell bodies (like in the midbrain, hypothalamus, etc.) or in areas where you would mostly see diffuse fiber staining (like in the striatum)? Because I've started staining for TH in rodent brain and the staining pattern in the striatum is diffuse (i.e. the whole striatum is darker than the surrounding cortex, but nothing like cell bodies...because it's mostly terminal projections- correct me if I'm wrong, but I wouldn't expect to see many/any cell bodies labeled with TH in the striatum), whereas it clearly picks up cell bodies in regions that contain dopamine producing neurons. > > So if you were only staining rostral forebrain sections (like the striatum), I don't know if you should expect to see cell bodies, just diffuse fibers. If you're not already doing it, maybe you should include more caudal sections in your IHC (sections containing the arcuate nucleus or ventral tegmental area, etc. would be great)...that way you'd know if you're detecting cells in those areas and the weak staining in striatum could be fibers that just need amplification? Sorry if I'm misinterpreting your post about the weak staining...but it would be an easy thing to overlook if you didn't have sections with cell bodies in them... > > Finally, I've only used a sheep anti-TH IgG from Novus Biological and it worked beautifully on the first time in a non-traditional rodent species (1:1,000 with fluorescence, so I imagine even more dilute concentrations should work with enzyme-mediated chromagen detection)...so I agree with Geoff McAuliffe that getting good results with TH should be easy... > > If you're interested I can email you my protocol, it's not too different from what you described....but what detection system are you using? Fluorescence, DAB, other? Because you didn't mention any pretreatments like HRP inactivation or quenching unreacted aldehydes...maybe if you give more particulars to your methods, others could provide more tips? > > > Good luck with the trouble shooting, > Adam > > Geoff McAuliffe wrote: Hi Johanna: > > Your proceedure sounds fine to me. I have never used the antibody in > question but getting good results with TH should be VERY easy. I use a > polyclonal TH from Pel-Freez in Rogers, AK, works great on both mouse > and rat brains. Assuming the brains were not overfixed (24 hours could > be too long) and the antibody is still good I would make sure all of my > solutions were properly labeled. Also, is your detection system OK? You > could have great binding but if the detection system is not working > properly you will never know it. > > Geoff > > Jackson, Johanna wrote: > > >Dear All, > > > >I am trying to stain dopaminergic neurons in the striatum of the adult rat brain which has been perfused with 4% PFA, washed and then cryoprotected in 20% sucrose, before being sectioned (10um) on a cryostat. I pretreat the sections with 0.1% Triton-X and block using serum. I am using the monoclonal anti-tyrosine hydroxylase antibody from Sigma (T1299). Sigma recommend a working concentration of 1:2000 however I have had no luck with this staining. I just a weak staining all over the brain with no specificity in the striatum. > > > >Does anyone have a protocol using this particular antibody? I have seen protocols with anti-TH from other companies which follow a similar method to what I have described above, however they do not seem to work for the Sigma antibody. > > > >Any help would be greatly appreciated! > > > >Thanks! > > > >Jo > > > > > >Stem Cell Imaging > >MRC Clinical Sciences Centre > >Imperial College London > >Hammersmith Hospital Campus > >Du Cane Road > >London > >W12 0NN > >0208 3833796 > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583; fax: -4029 > mcauliff@umdnj.edu > ********************************************** > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Yahoo! Messenger with Voice. PC-to-Phone calls for ridiculously low rates. > > ------------------------------ > > Message: 16 > Date: Tue, 23 May 2006 23:01:54 -0400 > From: "Katri Tuomala" > Subject: Re: [Histonet] Re: Fixatives > To: > Message-ID: <006e01c67ede$69e28450$6a9a9618@Katri> > Content-Type: text/plain; format=flowed; charset="iso-8859-1"; > reply-type=original > > I have always read with respect Dr. Richmond's opinions, but here I have to > disagree. > > Surely the bottle, that the specimen comes in, is labelled with what ever > the fixative is, along with the pertinent patient information. It should be > just as simple to say " specimen received in formalin (Bouins, Zenkers > etc.)". There is no need and it is not advisable to smell it. > > It is an interesting mindset among many, not all, pathologist to insist in > processing all tissues the same day (TAT!) ending up with inadequately fixed > and consequently poorly processed tissue, which we technologist then have to > struggle to produce well stained, wrinkle free, representative sections. As > an immuno tech I can testify for even bigger problems in producing reliable > results with immunohistochemical procedures. > > For purposes of immunostaining, it is important to know the time of fixation > in formalin, adequate or not (24 hours is considered adequate, not 4 or even > 8), so that we know what we are faced with and sometimes method can be > adjusted to fit the fixation time. I would prefer doing immuno stains on a > specimen, that is "overfixed" in formalin rather than underfixed. > > If I ever ended up with breast cancer, I would make sure, that at least one > tumor section got fixed 24 hours. > > This is my Tuesday Rant! > > Katri > Katri Tuomala > Hamilton, Ontario, Canada > > > > > ----- Original Message ----- > From: > To: > Sent: Tuesday, May 23, 2006 4:22 AM > Subject: [Histonet] Re: Fixatives > > > > Patti Loykasek at PhenoPath Laboratories notes >>We are a reference lab > > and > > receive specimens from all over the USA. One of my "pet peeves" is that it > > is > > rare to see in the report exactly what type of fixative the specimen was > > received in or subsequently processed in. I know we have no standard form > > of > > reporting, but it just seems like best practice to me to include this > > information on > > the report. One of my favorites is "...received in fixative..." - not very > > helpful.<< > > > > That's exactly the phrase I use in my gross descriptions, and for a very > > good > > reason. I'm not about to stick my nose into every specimen bottle to > > verify > > that it contains formalin and not alcohol, water, or pine-scented floor > > disinfectant (used at one hospital I know as a "fixative" for placentas). > > I'm willing > > to smell-test a very occasional container where I'm suspicious that the > > wrong > > fixative has been used, but not every time! > > > > Time of fixation is the dead horse in the middle of the living room > > floor - > > nobody wants to hear that the HER2 immunostain for breast cancer requires > > overnight fixation, for example. > > > > Bob Richmond > > Gastonia NC > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 17 > Date: Wed, 24 May 2006 08:01:59 +0100 > From: Kemlo Rogerson > Subject: RE: [Histonet] Formalin on skin > To: "'Jackie M O'Connor'" , Chris Pomajzl > > Cc: HISTONET > Message-ID: > > Content-Type: text/plain > > Interestingly fish medication contains malachite green and formalin to cure > ulcers and to kill parasites; though oddly I think they are caused by the > same thing. Fish harbor aeromonas and under extremes succumb to ulcers; on > recovery invariably flagellated protozoa infest the wound and keep it > 'open', the fish waterlogs. > > Formalin kills these parasites but not as well as metronidazole; though > getting the medicine can be embarrassing. Have I gone off piste? > > Kemlo Rogerson > Pathology Manager > Ext 3311 > DD 01934 647057 > Mob 07749 754194 > > Education is not the filling of a pail, but the lighting of a fire. --W. B. > Yeats > > > > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on its > contents: to do so is strictly prohibited and may be unlawful. Please inform > me that this message has gone astray before deleting it. Thank you for your > co-operation > > > > > > > > ------------------------------ > > Message: 18 > Date: Wed, 24 May 2006 08:06:24 +0100 > From: Kemlo Rogerson > Subject: RE: [Histonet] Formalin on skin > To: "'Bryan Llewellyn'" , Histonet > > Message-ID: > > Content-Type: text/plain > > Whilst I accept your premise that the mutagenic properties of formalin is > low; anything that causes cell death, and formalin certainly does that, > increases the mitotic activity of replacement cells and thereby increases > the risk of an abnormality. > > Personally I have excluded pregnant woman from the presence of formalin and > try to have zero tolerance for trying to fix my Staff in its fumes by using > extraction, monitoring and protective gloves, etc. > > Kemlo Rogerson > Pathology Manager > Ext 3311 > DD 01934 647057 > Mob 07749 754194 > > Education is not the filling of a pail, but the lighting of a fire. --W. B. > Yeats > > > > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on its > contents: to do so is strictly prohibited and may be unlawful. Please inform > me that this message has gone astray before deleting it. Thank you for your > co-operation > > > > > > > > ------------------------------ > > Message: 19 > Date: Wed, 24 May 2006 08:09:43 +0100 > From: Kemlo Rogerson > Subject: RE: [Histonet] Formalin on skin > To: "'gu.lang@gmx.at'" , "Histonetliste > (Histonetliste)" > Message-ID: > > Content-Type: text/plain > > Maybe it's not the physical drenching of the skin and the effects on that > you should worry about but the fact that formalin is now on a larger warm > surface area and the known dangers of formalin inhalation on the lung; best > not to experiment on Staff IMHO. > > Kemlo Rogerson > Pathology Manager > Ext 3311 > DD 01934 647057 > Mob 07749 754194 > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on its > contents: to do so is strictly prohibited and may be unlawful. Please inform > me that this message has gone astray before deleting it. Thank you for your > co-operation > > > > > > > > ------------------------------ > > Message: 20 > Date: Wed, 24 May 2006 11:39:19 +0200 > From: Melanie Black > Subject: [Histonet] RE: Hoechst staining method > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset="us-ascii" ; format="flowed" > > Hi Tracey > > Some years ago, I looked into fluorescent nuclear counterstains, > including dapi, propidium iodide and HOECHST. Heochst is also known > as bisBenzimide. > > Method: > Mix 1mg bisBemzimide (Sigma B2883) with 1ml Dist water. Take 4.0 > micro litres of this stock and dilute with 1ml PBS pH 7.4.Apply to > sections for 10 mins, wash 3 x in PBS. Fluorescence is bright blue, > viewed with a UV filter 340 nm. > > Stain reference: > Latt,SA and Stetten GJ. Histochem. Cytochem 24,24 (1976) > Araki, T et al, Histochem. 87,331 (1987) > > Regards > Melanie Black > > > > > > > Hi, > We are doing an immunofluorescence research project for podocytes. > The urine cytospins are to be counterstained with Hoescht. We have > never used it - can someone recommend a concentration, diluent and > staining time that we could start with. Thanks in advance. > > Tracey > > -- > Cardiovascular Research Unit > Div. of Cardiothoracic Surgery > Chris Barnard Building > University of Cape Town > Anzio Road > Observatory > 7925 > Republic of South Africa > > Tel +27 21 406-6589 > Cel +27 82 469-3352 > Fax +27 21 448-5935 > > > > ------------------------------ > > Message: 21 > Date: Wed, 24 May 2006 07:17:18 -0400 > From: "Weems, Joyce" > Subject: RE: [Histonet] Re: Fixatives > To: "Katri Tuomala" , > > Message-ID: > <1CD6831EB9B26D45B0A3EAA79F7EBD320263E103@sjhaexc02.sjha.org> > Content-Type: text/plain; charset="utf-8" > > "Surely the bottle, that the specimen comes in, is labelled with what ever > the fixative is, along with the pertinent patient information. It should be > just as simple to say " specimen received in formalin (Bouins, Zenkers > etc.)". There is no need and it is not advisable to smell it." > > If this statement were true, there would be no question. However, not > everyone uses prefilled containers, and the labeling is not always ideal. I'm > sure this is the situation to which Dr. Bob refers...j > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital of Atlanta > 404-851-7376 > 404-851-7831 - Fax > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Katri Tuomala > Sent: Tue 5/23/2006 11:01 PM > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Re: Fixatives > > I have always read with respect Dr. Richmond's opinions, but here I have to > disagree. > > Surely the bottle, that the specimen comes in, is labelled with what ever > the fixative is, along with the pertinent patient information. It should be > just as simple to say " specimen received in formalin (Bouins, Zenkers > etc.)". There is no need and it is not advisable to smell it. > > It is an interesting mindset among many, not all, pathologist to insist in > processing all tissues the same day (TAT!) ending up with inadequately fixed > and consequently poorly processed tissue, which we technologist then have to > struggle to produce well stained, wrinkle free, representative sections. As > an immuno tech I can testify for even bigger problems in producing reliable > results with immunohistochemical procedures. > > For purposes of immunostaining, it is important to know the time of fixation > in formalin, adequate or not (24 hours is considered adequate, not 4 or even > 8), so that we know what we are faced with and sometimes method can be > adjusted to fit the fixation time. I would prefer doing immuno stains on a > specimen, that is "overfixed" in formalin rather than underfixed. > > If I ever ended up with breast cancer, I would make sure, that at least one > tumor section got fixed 24 hours. > > This is my Tuesday Rant! > > Katri > Katri Tuomala > Hamilton, Ontario, Canada > > > > > ----- Original Message ----- > From: > To: > Sent: Tuesday, May 23, 2006 4:22 AM > Subject: [Histonet] Re: Fixatives > > > > Patti Loykasek at PhenoPath Laboratories notes >>We are a reference lab > > and > > receive specimens from all over the USA. One of my "pet peeves" is that it > > is > > rare to see in the report exactly what type of fixative the specimen was > > received in or subsequently processed in. I know we have no standard form > > of > > reporting, but it just seems like best practice to me to include this > > information on > > the report. One of my favorites is "...received in fixative..." - not very > > helpful.<< > > > > That's exactly the phrase I use in my gross descriptions, and for a very > > good > > reason. I'm not about to stick my nose into every specimen bottle to > > verify > > that it contains formalin and not alcohol, water, or pine-scented floor > > disinfectant (used at one hospital I know as a "fixative" for placentas). > > I'm willing > > to smell-test a very occasional container where I'm suspicious that the > > wrong > > fixative has been used, but not every time! > > > > Time of fixation is the dead horse in the middle of the living room > > floor - > > nobody wants to hear that the HER2 immunostain for breast cancer requires > > overnight fixation, for example. > > > > Bob Richmond > > Gastonia NC > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. > > ------------------------------ > > Message: 22 > Date: Wed, 24 May 2006 08:20:53 -0400 > From: Denise Crowley > Subject: [Histonet] cytochrome c oxidase and succinate dehydrogenase > To: histonet@lists.utsouthwestern.edu > Message-ID: <5CE60D25-D94D-4942-8080-49E931647809@mit.edu> > Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed > > I have been asked to perform these enzyme stains on murine heart. > Since this is new to me, I'm asking the experts for any favorite > protocols. Even better, any commercially available kits. > > I also have a question about the frozen sectioning. In the clinical > setting, we always froze the muscle biopsy without OCT. Is there a > good reason for this? I think that filling the heart with diluted > OCT will produce a nicer looking section, but I don't want to > compromise the staining. > > Denise Crowley > Facility Manager Histology > Center for Cancer Research > Massachusetts Institute of Technology > 40 Ames St. E17-230 > Cambridge MA 02139 > 617-258-8183 > dencrowl@mit.edu > > > > > > > ------------------------------ > > Message: 23 > Date: Wed, 24 May 2006 08:36:22 -0500 > From: "Horn, Hazel V" > Subject: RE: [Histonet] Sealing Paraffin Blocks > To: kerry.l.crabb@gsk.com, histonet@lists.utsouthwestern.edu > Message-ID: > <9AE8AA9E1F644B4AA6C155FB6FD51C63038BE0A3@EMAIL.archildrens.org> > Content-Type: text/plain; charset=us-ascii > > We used to do this years ago. We no longer seal blocks. In fact, I > believe you cut away more on a sealed block if the block requires > recutting. > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Histology > Arkansas Children's Hospital > 800 Marshall Slot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > fax 501.364.3912 > > visit us on the web at: www.archildrens.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > kerry.l.crabb@gsk.com > Sent: Tuesday, May 23, 2006 5:45 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Sealing Paraffin Blocks > > What is the current opinion and practice on sealing paraffin blocks > prior > to filing/archiving them? What is the pro and con in your opinion? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -------------------------------------------------------------------------- ---- > The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. > ============================================================================ == > > > > > ------------------------------ > > Message: 24 > Date: Wed, 24 May 2006 08:40:38 -0500 > From: "Horn, Hazel V" > Subject: RE: [Histonet] Re: Fixatives > To: "Weems, Joyce" , "Katri Tuomala" > , histonet@lists.utsouthwestern.edu > Message-ID: > <9AE8AA9E1F644B4AA6C155FB6FD51C63038BE0A4@EMAIL.archildrens.org> > Content-Type: text/plain; charset=us-ascii > > And not all bottles say exactly what the formalin fixative is. Some > labels are just a formalin warning label. Our fixative is Zinc buffered > formalin. Most would probably assume the label was for 10% NBF when in > fact it is not. We use ARUP Labs for a few tests we do not do and > their requisition asks what fixative was used. > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Histology > Arkansas Children's Hospital > 800 Marshall Slot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > fax 501.364.3912 > > visit us on the web at: www.archildrens.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, > Joyce > Sent: Wednesday, May 24, 2006 6:17 AM > To: Katri Tuomala; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Re: Fixatives > > "Surely the bottle, that the specimen comes in, is labelled with what > ever > the fixative is, along with the pertinent patient information. It should > be > just as simple to say " specimen received in formalin (Bouins, Zenkers > etc.)". There is no need and it is not advisable to smell it." > > If this statement were true, there would be no question. However, > not > everyone uses prefilled containers, and the labeling is not always > ideal. I'm > sure this is the situation to which Dr. Bob refers...j > > Joyce Weems > Pathology Manager > Saint Joseph's Hospital of Atlanta > 404-851-7376 > 404-851-7831 - Fax > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Katri > Tuomala > Sent: Tue 5/23/2006 11:01 PM > To: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Re: Fixatives > > I have always read with respect Dr. Richmond's opinions, but here I have > to > disagree. > > Surely the bottle, that the specimen comes in, is labelled with what > ever > the fixative is, along with the pertinent patient information. It should > be > just as simple to say " specimen received in formalin (Bouins, Zenkers > etc.)". There is no need and it is not advisable to smell it. > > It is an interesting mindset among many, not all, pathologist to insist > in > processing all tissues the same day (TAT!) ending up with inadequately > fixed > and consequently poorly processed tissue, which we technologist then > have to > struggle to produce well stained, wrinkle free, representative sections. > As > an immuno tech I can testify for even bigger problems in producing > reliable > results with immunohistochemical procedures. > > For purposes of immunostaining, it is important to know the time of > fixation > in formalin, adequate or not (24 hours is considered adequate, not 4 or > even > 8), so that we know what we are faced with and sometimes method can be > adjusted to fit the fixation time. I would prefer doing immuno stains on > a > specimen, that is "overfixed" in formalin rather than underfixed. > > If I ever ended up with breast cancer, I would make sure, that at least > one > tumor section got fixed 24 hours. > > This is my Tuesday Rant! > > Katri > Katri Tuomala > Hamilton, Ontario, Canada > > > > > ----- Original Message ----- > From: > To: > Sent: Tuesday, May 23, 2006 4:22 AM > Subject: [Histonet] Re: Fixatives > > > > Patti Loykasek at PhenoPath Laboratories notes >>We are a reference > lab > > and > > receive specimens from all over the USA. One of my "pet peeves" is > that it > > is > > rare to see in the report exactly what type of fixative the specimen > was > > received in or subsequently processed in. I know we have no standard > form > > of > > reporting, but it just seems like best practice to me to include this > > information on > > the report. One of my favorites is "...received in fixative..." - not > very > > helpful.<< > > > > That's exactly the phrase I use in my gross descriptions, and for a > very > > good > > reason. I'm not about to stick my nose into every specimen bottle to > > verify > > that it contains formalin and not alcohol, water, or pine-scented > floor > > disinfectant (used at one hospital I know as a "fixative" for > placentas). > > I'm willing > > to smell-test a very occasional container where I'm suspicious that > the > > wrong > > fixative has been used, but not every time! > > > > Time of fixation is the dead horse in the middle of the living room > > floor - > > nobody wants to hear that the HER2 immunostain for breast cancer > requires > > overnight fixation, for example. > > > > Bob Richmond > > Gastonia NC > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > Confidentiality Notice ** The information contained in this message may > be privileged and is confidential information intended for the use of > the addressee listed above. If you are neither the intended recipient > nor the employee or agent responsible for delivering this message to the > intended recipient, you are hereby notified that any disclosure, > copying, distribution or the taking of any action in reliance on the > contents of this information is strictly prohibited. If you have > received this communication in error, please notify us immediately by > replying to the message and deleting it from your computer. Thank you. > Saint Joseph's Health System, Inc. > > -------------------------------------------------------------------------- ---- > The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. > ============================================================================ == > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 30, Issue 35 > **************************************** > From rjbuesa <@t> yahoo.com Wed May 24 12:03:49 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 24 12:03:55 2006 Subject: [Histonet] Sealing Paraffin Blocks In-Reply-To: Message-ID: <20060524170349.78362.qmail@web61212.mail.yahoo.com> Kerry: Sealing the surface of paraffin blocks is advisable to prevent tissue oxidation, fungus growth, insects bitting / chewing into the tissue, shrinkage from drying, and the prevention of all of that is good, BUT it is a time consuming and costly operation. In labs with large work volume it is almost imposible to do on a regular basis without investing a lot of time and effort. I have done it but just for control blocks or some interesting cases that the pathologists wanted to keep for years as part of our teching activities, otherwise, in spite of the potential advantages, we did not do it for routine cases. As in many things, a balance has to be found. Hope this will help you Ren? J. kerry.l.crabb@gsk.com wrote: What is the current opinion and practice on sealing paraffin blocks prior to filing/archiving them? What is the pro and con in your opinion? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. From ploykasek <@t> phenopath.com Wed May 24 12:18:39 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Wed May 24 12:18:56 2006 Subject: [Histonet] Re: Histonet Digest, Vol 30, Issue 35, re: formalin exposure In-Reply-To: <000501c67f53$0e37f840$64c809c0@tlabs> Message-ID: I'm all for the practice of using the least hazardous reagents available. However, there are many sides to this issue. Does the formalin substitute have an MSDS? Are you sure it is less hazardous than formalin or are the risks just being switched around a bit. How will the formalin substitute affect special stains, IHC, molecular testing, etc..? Are you using any FDA approved kits for any type of testing & does the kit specify what type of fixation the specimen should have? I know the HercepTest specifies fixation. Just a few issues to consider. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Hi, Formaldehyde is a carcinogen and is list a one of the top ten toxic > chemicals used in industry. It is proven to be a carcinogen in humans > mainly GI and liver. It is also an Immuno, Neuro, Reproductive and > Respiratory toxin. Not to mention a skin sensitizer. Anyone using > formaldehyde should minimize there exposure to it and if possible eliminate > it completely. At our lab, we will be switching to a formalin substitute. > Adam > ----- Original Message ----- > From: > To: > Sent: Wednesday, May 24, 2006 8:47 AM > Subject: Histonet Digest, Vol 30, Issue 35 > > >> Send Histonet mailing list submissions to >> histonet@lists.utsouthwestern.edu >> >> To subscribe or unsubscribe via the World Wide Web, visit >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> or, via email, send a message with subject or body 'help' to >> histonet-request@lists.utsouthwestern.edu >> >> You can reach the person managing the list at >> histonet-owner@lists.utsouthwestern.edu >> >> When replying, please edit your Subject line so it is more specific >> than "Re: Contents of Histonet digest..." >> >> >> Today's Topics: >> >> 1. Formalin on skin (Gudrun Lang) >> 2. (no subject) (Paul Cremers) >> 3. RE: Formalin on skin (Charles.Embrey) >> 4. Shirley on leave : 24 May 2006 (Wednesday) (Shirley PHUA) >> 5. Re: Formalin on skin (Bryan Llewellyn) >> 6. Re: Formalin on skin (Chris Pomajzl) >> 7. re:Hoechst staining... (Carl Hobbs) >> 8. Re: Formalin on skin (Jackie M O'Connor) >> 9. Fw: Source of Re: [Histonet] Incubation chamber (Jan Shivers) >> 10. GSH meeting (Shirley Powell) >> 11. Re: STAFF EVALUATIONS (Patti Loykasek) >> 12. Sealing Paraffin Blocks (kerry.l.crabb@gsk.com) >> 13. Re: Re: Fixatives (Patti Loykasek) >> 14. RE: DNA/RNA Extraction Kits for paraffinised tissues >> (Makhijani, Nalini S) >> 15. Re: tyrosine hydroxylase staining?? (Adam Perry) >> 16. Re: Re: Fixatives (Katri Tuomala) >> 17. RE: Formalin on skin (Kemlo Rogerson) >> 18. RE: Formalin on skin (Kemlo Rogerson) >> 19. RE: Formalin on skin (Kemlo Rogerson) >> 20. RE: Hoechst staining method (Melanie Black) >> 21. RE: Re: Fixatives (Weems, Joyce) >> 22. cytochrome c oxidase and succinate dehydrogenase (Denise Crowley) >> 23. RE: Sealing Paraffin Blocks (Horn, Hazel V) >> 24. RE: Re: Fixatives (Horn, Hazel V) >> >> >> ---------------------------------------------------------------------- >> >> Message: 1 >> Date: Tue, 23 May 2006 19:32:18 +0200 >> From: "Gudrun Lang" >> Subject: [Histonet] Formalin on skin >> To: "Histonetliste \(Histonetliste\)" >> >> Message-ID: <000101c67e8e$d7693550$eeeea8c0@SERVER01> >> Content-Type: text/plain; charset="us-ascii" >> >> Hi, >> Today a labassistent asked me, what would happen to her skin, if formalin >> swallowed over her arm. >> I said, the formalin would harden the skin, but only on the surface. She >> should wash the arm with plenty of water, and there will reamain no > negative >> reaction. It would not be dangerous for her health. >> >> Do you agree with me? Is there a cancer-risk after short contact with >> formalin? >> >> Greetings >> Gudrun Lang >> >> Histolab >> Akh Linz >> Austria >> >> >> >> >> ------------------------------ >> >> Message: 2 >> Date: Tue, 23 May 2006 13:35:58 -0400 >> From: "Paul Cremers" >> Subject: [Histonet] (no subject) >> To: histonet@lists.utsouthwestern.edu >> Message-ID: >> Content-Type: text/plain; format="flowed" >> >> >> Hello to all, >> >> Is there anyone looking for a histotech or two. My wife and I >> are wanting to move to the Boulder or Fort Collins, Colorado area. We >> are also interested in Portland, Salem, Corvalis and Eugene, Oregon. >> We are both HT certified and are wanting to start somewhere between >> mid-August and September 1. >> >> Thanks, >> >> Paul and Melissa Cremers >> >> >> ------------------------------ >> >> Message: 3 >> Date: Tue, 23 May 2006 12:56:15 -0500 >> From: "Charles.Embrey" >> Subject: RE: [Histonet] Formalin on skin >> To: , "Histonetliste \(Histonetliste\)" >> >> Message-ID: >> >> Content-Type: text/plain; charset="us-ascii" >> >> You are 100% correct. I have even reached a bare hand into formalin to >> pull out something before but washed with soap and water minutes after. >> I have not personally heard of formalin causing an increased risk of >> skin cancer however I am sure that there is a study somewhere that has >> probably associated some risk with L---O---N---G term exposure. Your >> labassistant should be perfectly safe. Just don't drink it and keep it >> out of the eyes :) >> >> Charles Embrey Jr. PA(ASCP) >> www.greyrealm.com >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun >> Lang >> Sent: Tuesday, May 23, 2006 12:32 PM >> To: Histonetliste (Histonetliste) >> Subject: [Histonet] Formalin on skin >> >> Hi, >> Today a labassistent asked me, what would happen to her skin, if >> formalin >> swallowed over her arm. >> I said, the formalin would harden the skin, but only on the surface. She >> should wash the arm with plenty of water, and there will reamain no >> negative >> reaction. It would not be dangerous for her health. >> >> Do you agree with me? Is there a cancer-risk after short contact with >> formalin? >> >> Greetings >> Gudrun Lang >> >> Histolab >> Akh Linz >> Austria >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> ------------------------------ >> >> Message: 4 >> Date: Wed, 24 May 2006 02:11:23 +0800 >> From: Shirley PHUA >> Subject: [Histonet] Shirley on leave : 24 May 2006 (Wednesday) >> To: histonet >> Message-ID: >> >> >> Content-Type: text/plain; charset=US-ASCII >> >> I will be out of the office from 24/05/2006 to 24/05/2006. >> >> I am away on 1 day leave. >> >> I will return on 25/05/2006 (Thursday). >> >> Pathologists : I will process your requests when I return. Otherwise, if >> urgent, please contact Henry. >> >> >> >> >> ------------------------------ >> >> Message: 5 >> Date: Tue, 23 May 2006 12:12:39 -0700 >> From: Bryan Llewellyn >> Subject: Re: [Histonet] Formalin on skin >> To: Histonet >> Message-ID: <001201c67e9c$dbefc680$130e4246@yourlk4rlmsu> >> Content-Type: text/plain; format=flowed; charset=iso-8859-1; >> reply-type=original >> >> Most of us old-timers have stuck our fingers in formalin or spilled it on >> ourselves on many occasions. I have never heard of anyone developing a >> tumour from it, though. It certainly doesn't appear to be common if it > does >> happen. In Canada some years ago we had a government program assisting >> people to inject urea-formaldehyde resin into the walls of houses as a >> retrofit insulation system. A few years later it was decided the formalin >> fumes given off were carcinogenic, so we had another program to remove it. >> That's about the only link to tumours I have heard of. >> >> One of the pathologists I used to work with many years ago was fond of >> quoting a paper he had once read which compared naso-pharyngeal tumour > rates >> among various groups. Pathologists had a lower rate than other people. > He >> always put that down to the formalin fumes they breathed in all the time. >> >> The real identified problem with formalin and skin contact is dermatitis. >> It isn't universal, but some people do get it with repeated contact, and >> once you develop the sensitivity to it, it doesn't go away. So wear > gloves. >> >> Bryan Llewellyn >> >> >> >> ----- Original Message ----- >> From: "Charles.Embrey" >> To: ; "Histonetliste (Histonetliste)" >> >> Sent: Tuesday, May 23, 2006 10:56 AM >> Subject: RE: [Histonet] Formalin on skin >> >> >> You are 100% correct. I have even reached a bare hand into formalin to >> pull out something before but washed with soap and water minutes after. >> I have not personally heard of formalin causing an increased risk of >> skin cancer however I am sure that there is a study somewhere that has >> probably associated some risk with L---O---N---G term exposure. Your >> labassistant should be perfectly safe. Just don't drink it and keep it >> out of the eyes :) >> >> Charles Embrey Jr. PA(ASCP) >> www.greyrealm.com >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun >> Lang >> Sent: Tuesday, May 23, 2006 12:32 PM >> To: Histonetliste (Histonetliste) >> Subject: [Histonet] Formalin on skin >> >> Hi, >> Today a labassistent asked me, what would happen to her skin, if >> formalin >> swallowed over her arm. >> I said, the formalin would harden the skin, but only on the surface. She >> should wash the arm with plenty of water, and there will reamain no >> negative >> reaction. It would not be dangerous for her health. >> >> Do you agree with me? Is there a cancer-risk after short contact with >> formalin? >> >> Greetings >> Gudrun Lang >> >> Histolab >> Akh Linz >> Austria >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> ------------------------------ >> >> Message: 6 >> Date: Tue, 23 May 2006 13:51:49 -0500 >> From: "Chris Pomajzl" >> Subject: Re: [Histonet] Formalin on skin >> To: "HISTONET" >> Message-ID: <004301c67e99$f31fa030$26fca8c0@CSP> >> Content-Type: text/plain; charset="iso-8859-1" >> >> I would imagine that if someone had open wounds or breaks in the skin, > there >> could be acute problems. But chronic long-term problems are unlikely with >> such a brief incident. >> >> People drink formaldehyde (or at least used to, wasn't it a preservative > in >> beer many years ago??), they smoke it (I've heard of people dipping joints >> in formaldehyde for a stronger buzz, never tried it myself), and we > breathe >> it in the lab every day. I believe that the carcinogenic effects are due > to >> long-term exposure in laboratory animals. I am not aware of any studies >> involving human subjects. >> >> Hope this helps. >> >> ----- Original Message ----- >> From: "Charles.Embrey" >> To: ; "Histonetliste (Histonetliste)" >> >> Sent: Tuesday, May 23, 2006 12:56 PM >> Subject: RE: [Histonet] Formalin on skin >> >> >> You are 100% correct. I have even reached a bare hand into formalin to >> pull out something before but washed with soap and water minutes after. >> I have not personally heard of formalin causing an increased risk of >> skin cancer however I am sure that there is a study somewhere that has >> probably associated some risk with L---O---N---G term exposure. Your >> labassistant should be perfectly safe. Just don't drink it and keep it >> out of the eyes :) >> >> Charles Embrey Jr. PA(ASCP) >> www.greyrealm.com >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun >> Lang >> Sent: Tuesday, May 23, 2006 12:32 PM >> To: Histonetliste (Histonetliste) >> Subject: [Histonet] Formalin on skin >> >> Hi, >> Today a labassistent asked me, what would happen to her skin, if >> formalin >> swallowed over her arm. >> I said, the formalin would harden the skin, but only on the surface. She >> should wash the arm with plenty of water, and there will reamain no >> negative >> reaction. It would not be dangerous for her health. >> >> Do you agree with me? Is there a cancer-risk after short contact with >> formalin? >> >> Greetings >> Gudrun Lang >> >> Histolab >> Akh Linz >> Austria >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> ------------------------------ >> >> Message: 7 >> Date: Tue, 23 May 2006 21:30:39 +0100 >> From: "Carl Hobbs" >> Subject: [Histonet] re:Hoechst staining... >> To: "Histonet" >> Message-ID: >> Content-Type: text/plain; charset="iso-8859-1" >> >> Of nuclei! >> Easy.....If you wish, check out this site for a protocol and images >> http://www.immunoportal.com/index.php >> Saves me fingers retyping ;-) >> It's a standard, tho'. No fancy stuff reqd. Lots of XPerienced people here >> to help, that I will always pay greatest respects to. >> Regards >> >> >> >> >> ------------------------------ >> >> Message: 8 >> Date: Tue, 23 May 2006 15:32:56 -0500 >> From: "Jackie M O'Connor" >> Subject: Re: [Histonet] Formalin on skin >> To: "Chris Pomajzl" >> Cc: HISTONET >> Message-ID: >> >> Content-Type: text/plain; charset="US-ASCII" >> >> Formaldehyde was a common ingredient of shampoos, and I remember reading >> the ingredients (with horror) on a bottle of Mr. Bubble bubble bath for >> kids (like 25 years ago). If you remember "Good Morning Vietnam" - they >> mentioned using formaldehyde to produce a better head on a glass of beer >> from the tap. >> I once worked with a pathologist who was allergic to formaldehyde. When >> he did the gross, we had to rinse all the specimens in H20 before he could >> examine them - yeah, that was fun. >> Jackie O' >> >> >> >> >> "Chris Pomajzl" >> Sent by: histonet-bounces@lists.utsouthwestern.edu >> 05/23/2006 01:51 PM >> >> To >> "HISTONET" >> cc >> >> Subject >> Re: [Histonet] Formalin on skin >> >> >> >> >> >> >> I would imagine that if someone had open wounds or breaks in the skin, >> there >> could be acute problems. But chronic long-term problems are unlikely with >> such a brief incident. >> >> People drink formaldehyde (or at least used to, wasn't it a preservative >> in >> beer many years ago??), they smoke it (I've heard of people dipping joints >> in formaldehyde for a stronger buzz, never tried it myself), and we >> breathe >> it in the lab every day. I believe that the carcinogenic effects are due >> to >> long-term exposure in laboratory animals. I am not aware of any studies >> involving human subjects. >> >> Hope this helps. >> >> ----- Original Message ----- >> From: "Charles.Embrey" >> To: ; "Histonetliste (Histonetliste)" >> >> Sent: Tuesday, May 23, 2006 12:56 PM >> Subject: RE: [Histonet] Formalin on skin >> >> >> You are 100% correct. I have even reached a bare hand into formalin to >> pull out something before but washed with soap and water minutes after. >> I have not personally heard of formalin causing an increased risk of >> skin cancer however I am sure that there is a study somewhere that has >> probably associated some risk with L---O---N---G term exposure. Your >> labassistant should be perfectly safe. Just don't drink it and keep it >> out of the eyes :) >> >> Charles Embrey Jr. PA(ASCP) >> www.greyrealm.com >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun >> Lang >> Sent: Tuesday, May 23, 2006 12:32 PM >> To: Histonetliste (Histonetliste) >> Subject: [Histonet] Formalin on skin >> >> Hi, >> Today a labassistent asked me, what would happen to her skin, if >> formalin >> swallowed over her arm. >> I said, the formalin would harden the skin, but only on the surface. She >> should wash the arm with plenty of water, and there will reamain no >> negative >> reaction. It would not be dangerous for her health. >> >> Do you agree with me? Is there a cancer-risk after short contact with >> formalin? >> >> Greetings >> Gudrun Lang >> >> Histolab >> Akh Linz >> Austria >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> ------------------------------ >> >> Message: 9 >> Date: Tue, 23 May 2006 16:55:27 -0500 >> From: "Jan Shivers" >> Subject: Fw: Source of Re: [Histonet] Incubation chamber >> To: "histonet" >> Message-ID: <012801c67eb3$9a32e3f0$a1065486@auxs.umn.edu> >> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; >> reply-type=response >> >> Years ago when my IHC lab was a fledgling entity with very little budget, > I >> used a 9x13" metal cake pan (with lid), wet paper towels for humidity in > the >> bottom, and disposable 1 ml pipets as the slide racks. They held two rows >> of 10 slides each. Just had to make sure my wet paper towels laid flat > (no >> bubbles), otherwise they'd make the pipets sit uneven. >> >> Jan Shivers >> UMN VDL >> >> ----- Original Message ----- >> From: "Gayle Callis" >> To: "Geoff McAuliffe" ; >> >> Sent: Tuesday, May 23, 2006 10:21 AM >> Subject: Source of Re: [Histonet] Incubation chamber >> >> >>> Evergreen Scientific has a 10 slide chamber, plastic, with lid. The >>> chamber is designed so slides are elevated, just add water to bottom of >>> wells for humidity. There are 3 chambers per package, very inexpensive. >>> You can stack them. They come either black plastic for fluorescence > work >>> OR clear. I have seen them sold other places to, maybe EMS is the > place. >>> Evergreen makes them. >>> >>> Very tidy little devices for low volume work >>> >>> At 08:06 AM 5/23/2006, you wrote: >>>> I use "Tupperware", or a generic equivalent. Slides are supported by > glass >>>> rods in the bottom of the container. Add a little water and seal. >>>> >>>> Geoff >>> >>> Gayle Callis >>> MT,HT,HTL(ASCP) >>> Research Histopathology Supervisor >>> Veterinary Molecular Biology >>> Montana State University - Bozeman >>> PO Box 173610 >>> Bozeman MT 59717-3610 >>> 406 994-6367 >>> 406 994-4303 (FAX) >>> >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> >> >> >> >> ------------------------------ >> >> Message: 10 >> Date: Tue, 23 May 2006 18:00:28 -0400 >> From: Shirley Powell >> Subject: [Histonet] GSH meeting >> To: histonet@lists.utsouthwestern.edu >> Message-ID: <01M2RQRHLNHW8WWEFM@Macon2.Mercer.edu> >> Content-Type: text/plain; charset=US-ASCII >> >> Reminder:::: >> >> >> The Georgia Society for Histotechnology and the Georgia Society of > Cytology >> will hold their 4th Annual Combined Scientific Symposium on Saturday, June >> 3, 2006, in Augusta, Georgia at the Marriott Augusta Hotel and Suites, 2 >> Tenth Street, Augusta, GA 30901. The phone number for reservations is > 1-706 >> 722-8900. >> >> I will email you a program upon request. I also have vendor information > for >> any who wish to exhibit. >> >> Thanks, >> >> Shirley Powell >> GSH Secretary/Membership Chair >> >> >> >> >> ------------------------------ >> >> Message: 11 >> Date: Tue, 23 May 2006 15:39:28 -0700 >> From: Patti Loykasek >> Subject: Re: [Histonet] STAFF EVALUATIONS >> To: Diana McCaig , histonet >> >> Message-ID: >> Content-Type: text/plain; charset="US-ASCII" >> >> Hi Diana. I would be interested in your sharing the info that you get > back. >> I can tell you what we do. I'll start off by saying we are constantly >> updating/revising our program. I feel like we do a better job each year. >> Some tasks are evaluated by observation & documented on a spreadsheet that >> contains all SOPs (by name) that a particular staff should know. I do have >> checklists for equipment that cover things from do staff know where on/off >> is, to the various functions of the equipment staff should know. For the >> histologists we evaluate H&E sections (both paraffin & FS) with criteria >> similar to the board registry & include some safety & clean up details. > I'm >> putting together a test to cover knowledge of general IHC & would like to >> get some photos of common stains acceptable & not for a test. A work in >> progress - always. >> Hope that helps a bit. >> >> >> Patti Loykasek BS, HTL, QIHC >> PhenoPath Laboratories >> Seattle, WA >> >> >> >> >>> Does anyone do an annual evaluation of staff ability to perform certain > tasks? >>> Diana McCaig 519-352-6401 (6604) >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> ------------------------------------------------------------------------- >> This e-mail message, including any attachments, is for the sole use of >> the intended recipients and may contain privileged information. Any >> unauthorized review, use, disclosure or distribution is prohibited. If >> you are not the intended recipient, please contact the sender by e-mail >> and destroy all copies of the original message, or you may call PhenoPath >> Laboratories, Seattle, WA U.S.A. at (206) 374-9000. >> >> >> >> >> ------------------------------ >> >> Message: 12 >> Date: Tue, 23 May 2006 18:45:19 -0400 >> From: kerry.l.crabb@gsk.com >> Subject: [Histonet] Sealing Paraffin Blocks >> To: histonet@lists.utsouthwestern.edu >> Message-ID: >> >> Content-Type: text/plain; charset=us-ascii >> >> What is the current opinion and practice on sealing paraffin blocks prior >> to filing/archiving them? What is the pro and con in your opinion? >> >> ------------------------------ >> >> Message: 13 >> Date: Tue, 23 May 2006 15:46:19 -0700 >> From: Patti Loykasek >> Subject: Re: [Histonet] Re: Fixatives >> To: >> Message-ID: >> Content-Type: text/plain; charset="US-ASCII" >> >> Dr. Richmond, I always appreciate your views and comments. I do think if a >> bottle comes to the lab with a label noting fixative it could be dictated > as >> "...received in container labeled ..." or ",,,received in unlabeled >> container..", this is what was done at previous facilities I have worked, >> but perhaps is not the norm. >> I do agree that the elephant in the room is how poor or improper >> fixation/processing affects everything downstream. I could go on & on > about >> this issue. I feel that CAP nor anyone else does very little to address > this >> issue. Everyone turns a bit of a blind eye to it. >> Thanks for the input. >> >> >> Patti Loykasek BS, HTL, QIHC >> PhenoPath Laboratories >> Seattle, WA >> >> >> >> >> >>> Patti Loykasek at PhenoPath Laboratories notes >>We are a reference lab > and >>> receive specimens from all over the USA. One of my "pet peeves" is that > it is >>> rare to see in the report exactly what type of fixative the specimen was >>> received in or subsequently processed in. I know we have no standard > form of >>> reporting, but it just seems like best practice to me to include this >>> information on >>> the report. One of my favorites is "...received in fixative..." - not > very >>> helpful.<< >>> >>> That's exactly the phrase I use in my gross descriptions, and for a very > good >>> reason. I'm not about to stick my nose into every specimen bottle to > verify >>> that it contains formalin and not alcohol, water, or pine-scented floor >>> disinfectant (used at one hospital I know as a "fixative" for > placentas). I'm >>> willing >>> to smell-test a very occasional container where I'm suspicious that the > wrong >>> fixative has been used, but not every time! >>> >>> Time of fixation is the dead horse in the middle of the living room > floor - >>> nobody wants to hear that the HER2 immunostain for breast cancer > requires >>> overnight fixation, for example. >>> >>> Bob Richmond >>> Gastonia NC >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> ------------------------------------------------------------------------- >> This e-mail message, including any attachments, is for the sole use of >> the intended recipients and may contain privileged information. Any >> unauthorized review, use, disclosure or distribution is prohibited. If >> you are not the intended recipient, please contact the sender by e-mail >> and destroy all copies of the original message, or you may call PhenoPath >> Laboratories, Seattle, WA U.S.A. at (206) 374-9000. >> >> >> >> >> ------------------------------ >> >> Message: 14 >> Date: Tue, 23 May 2006 17:09:02 -0700 >> From: "Makhijani, Nalini S" >> Subject: RE: [Histonet] DNA/RNA Extraction Kits for paraffinised >> tissues >> To: "Shirley PHUA" , >> >> Message-ID: >> <715FA772CEA81A47B1A762AB6E45123A0111B549@VHAV22MSGA3.v22.med.va.gov> >> Content-Type: text/plain; charset="US-ASCII" >> >> Ambion advertises a kit, "RecoverAll", for total RNA and DNA extraction >> from FFPE tissues. I've never used it, but you could get more >> information from their web site: www.ambion.com/prod/recoverall. >> Hope this helps! >> >> Nalini Makhijani, M.S. >> Research Biologist >> VA Greater Los Angeles Healthcare System >> >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley >> PHUA >> Sent: Monday, May 22, 2006 7:32 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] DNA/RNA Extraction Kits for paraffinised tissues >> >> Dear All >> >> Have anyone heard of any DNA/RNA extraction kits for formalin-fixed, >> paraffinised tissues? I will greatly appreciate if you can share some of >> >> your feedbacks. Were your yields good? >> >> Thanks in advance! >> >> Shirley Phua >> Histopathology Laboratory >> Centre for Forensic Medicine >> Health Sciences Authority >> DID : 62130654 >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> ------------------------------ >> >> Message: 15 >> Date: Tue, 23 May 2006 17:17:35 -0700 (PDT) >> From: Adam Perry >> Subject: Re: [Histonet] tyrosine hydroxylase staining?? >> To: histonet@lists.utsouthwestern.edu >> Message-ID: <20060524001735.22829.qmail@web30413.mail.mud.yahoo.com> >> Content-Type: text/plain; charset=iso-8859-1 >> >> Hey Johanna, >> >> Just curious...when you say you get weak, diffuse staining all over the > brain, is that in sections that should contain dopaminergic cell bodies > (like in the midbrain, hypothalamus, etc.) or in areas where you would > mostly see diffuse fiber staining (like in the striatum)? Because I've > started staining for TH in rodent brain and the staining pattern in the > striatum is diffuse (i.e. the whole striatum is darker than the surrounding > cortex, but nothing like cell bodies...because it's mostly terminal > projections- correct me if I'm wrong, but I wouldn't expect to see many/any > cell bodies labeled with TH in the striatum), whereas it clearly picks up > cell bodies in regions that contain dopamine producing neurons. >> >> So if you were only staining rostral forebrain sections (like the > striatum), I don't know if you should expect to see cell bodies, just > diffuse fibers. If you're not already doing it, maybe you should include > more caudal sections in your IHC (sections containing the arcuate nucleus or > ventral tegmental area, etc. would be great)...that way you'd know if you're > detecting cells in those areas and the weak staining in striatum could be > fibers that just need amplification? Sorry if I'm misinterpreting your post > about the weak staining...but it would be an easy thing to overlook if you > didn't have sections with cell bodies in them... >> >> Finally, I've only used a sheep anti-TH IgG from Novus Biological and it > worked beautifully on the first time in a non-traditional rodent species > (1:1,000 with fluorescence, so I imagine even more dilute concentrations > should work with enzyme-mediated chromagen detection)...so I agree with > Geoff McAuliffe that getting good results with TH should be easy... >> >> If you're interested I can email you my protocol, it's not too different > from what you described....but what detection system are you using? > Fluorescence, DAB, other? Because you didn't mention any pretreatments like > HRP inactivation or quenching unreacted aldehydes...maybe if you give more > particulars to your methods, others could provide more tips? >> >> >> Good luck with the trouble shooting, >> Adam >> >> Geoff McAuliffe wrote: Hi Johanna: >> >> Your proceedure sounds fine to me. I have never used the antibody in >> question but getting good results with TH should be VERY easy. I use a >> polyclonal TH from Pel-Freez in Rogers, AK, works great on both mouse >> and rat brains. Assuming the brains were not overfixed (24 hours could >> be too long) and the antibody is still good I would make sure all of my >> solutions were properly labeled. Also, is your detection system OK? You >> could have great binding but if the detection system is not working >> properly you will never know it. >> >> Geoff >> >> Jackson, Johanna wrote: >> >>> Dear All, >>> >>> I am trying to stain dopaminergic neurons in the striatum of the adult > rat brain which has been perfused with 4% PFA, washed and then cryoprotected > in 20% sucrose, before being sectioned (10um) on a cryostat. I pretreat the > sections with 0.1% Triton-X and block using serum. I am using the > monoclonal anti-tyrosine hydroxylase antibody from Sigma (T1299). Sigma > recommend a working concentration of 1:2000 however I have had no luck with > this staining. I just a weak staining all over the brain with no > specificity in the striatum. >>> >>> Does anyone have a protocol using this particular antibody? I have seen > protocols with anti-TH from other companies which follow a similar method to > what I have described above, however they do not seem to work for the Sigma > antibody. >>> >>> Any help would be greatly appreciated! >>> >>> Thanks! >>> >>> Jo >>> >>> >>> Stem Cell Imaging >>> MRC Clinical Sciences Centre >>> Imperial College London >>> Hammersmith Hospital Campus >>> Du Cane Road >>> London >>> W12 0NN >>> 0208 3833796 >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >>> >>> >> >> >> -- >> -- >> ********************************************** >> Geoff McAuliffe, Ph.D. >> Neuroscience and Cell Biology >> Robert Wood Johnson Medical School >> 675 Hoes Lane, Piscataway, NJ 08854 >> voice: (732)-235-4583; fax: -4029 >> mcauliff@umdnj.edu >> ********************************************** >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> --------------------------------- >> Yahoo! Messenger with Voice. PC-to-Phone calls for ridiculously low rates. >> >> ------------------------------ >> >> Message: 16 >> Date: Tue, 23 May 2006 23:01:54 -0400 >> From: "Katri Tuomala" >> Subject: Re: [Histonet] Re: Fixatives >> To: >> Message-ID: <006e01c67ede$69e28450$6a9a9618@Katri> >> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; >> reply-type=original >> >> I have always read with respect Dr. Richmond's opinions, but here I have > to >> disagree. >> >> Surely the bottle, that the specimen comes in, is labelled with what ever >> the fixative is, along with the pertinent patient information. It should > be >> just as simple to say " specimen received in formalin (Bouins, Zenkers >> etc.)". There is no need and it is not advisable to smell it. >> >> It is an interesting mindset among many, not all, pathologist to insist in >> processing all tissues the same day (TAT!) ending up with inadequately > fixed >> and consequently poorly processed tissue, which we technologist then have > to >> struggle to produce well stained, wrinkle free, representative sections. > As >> an immuno tech I can testify for even bigger problems in producing > reliable >> results with immunohistochemical procedures. >> >> For purposes of immunostaining, it is important to know the time of > fixation >> in formalin, adequate or not (24 hours is considered adequate, not 4 or > even >> 8), so that we know what we are faced with and sometimes method can be >> adjusted to fit the fixation time. I would prefer doing immuno stains on a >> specimen, that is "overfixed" in formalin rather than underfixed. >> >> If I ever ended up with breast cancer, I would make sure, that at least > one >> tumor section got fixed 24 hours. >> >> This is my Tuesday Rant! >> >> Katri >> Katri Tuomala >> Hamilton, Ontario, Canada >> >> >> >> >> ----- Original Message ----- >> From: >> To: >> Sent: Tuesday, May 23, 2006 4:22 AM >> Subject: [Histonet] Re: Fixatives >> >> >>> Patti Loykasek at PhenoPath Laboratories notes >>We are a reference lab >>> and >>> receive specimens from all over the USA. One of my "pet peeves" is that > it >>> is >>> rare to see in the report exactly what type of fixative the specimen was >>> received in or subsequently processed in. I know we have no standard > form >>> of >>> reporting, but it just seems like best practice to me to include this >>> information on >>> the report. One of my favorites is "...received in fixative..." - not > very >>> helpful.<< >>> >>> That's exactly the phrase I use in my gross descriptions, and for a very >>> good >>> reason. I'm not about to stick my nose into every specimen bottle to >>> verify >>> that it contains formalin and not alcohol, water, or pine-scented floor >>> disinfectant (used at one hospital I know as a "fixative" for > placentas). >>> I'm willing >>> to smell-test a very occasional container where I'm suspicious that the >>> wrong >>> fixative has been used, but not every time! >>> >>> Time of fixation is the dead horse in the middle of the living room >>> floor - >>> nobody wants to hear that the HER2 immunostain for breast cancer > requires >>> overnight fixation, for example. >>> >>> Bob Richmond >>> Gastonia NC >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> ------------------------------ >> >> Message: 17 >> Date: Wed, 24 May 2006 08:01:59 +0100 >> From: Kemlo Rogerson >> Subject: RE: [Histonet] Formalin on skin >> To: "'Jackie M O'Connor'" , Chris Pomajzl >> >> Cc: HISTONET >> Message-ID: >> >> Content-Type: text/plain >> >> Interestingly fish medication contains malachite green and formalin to > cure >> ulcers and to kill parasites; though oddly I think they are caused by the >> same thing. Fish harbor aeromonas and under extremes succumb to ulcers; on >> recovery invariably flagellated protozoa infest the wound and keep it >> 'open', the fish waterlogs. >> >> Formalin kills these parasites but not as well as metronidazole; though >> getting the medicine can be embarrassing. Have I gone off piste? >> >> Kemlo Rogerson >> Pathology Manager >> Ext 3311 >> DD 01934 647057 >> Mob 07749 754194 >> >> Education is not the filling of a pail, but the lighting of a fire. --W. > B. >> Yeats >> >> >> >> >> This e-mail is confidential and privileged. If you are not the intended >> recipient please accept my apologies; please do not disclose, copy or >> distribute information in this e-mail or take any action in reliance on > its >> contents: to do so is strictly prohibited and may be unlawful. Please > inform >> me that this message has gone astray before deleting it. Thank you for > your >> co-operation >> >> >> >> >> >> >> >> ------------------------------ >> >> Message: 18 >> Date: Wed, 24 May 2006 08:06:24 +0100 >> From: Kemlo Rogerson >> Subject: RE: [Histonet] Formalin on skin >> To: "'Bryan Llewellyn'" , Histonet >> >> Message-ID: >> >> Content-Type: text/plain >> >> Whilst I accept your premise that the mutagenic properties of formalin is >> low; anything that causes cell death, and formalin certainly does that, >> increases the mitotic activity of replacement cells and thereby increases >> the risk of an abnormality. >> >> Personally I have excluded pregnant woman from the presence of formalin > and >> try to have zero tolerance for trying to fix my Staff in its fumes by > using >> extraction, monitoring and protective gloves, etc. >> >> Kemlo Rogerson >> Pathology Manager >> Ext 3311 >> DD 01934 647057 >> Mob 07749 754194 >> >> Education is not the filling of a pail, but the lighting of a fire. --W. > B. >> Yeats >> >> >> >> >> This e-mail is confidential and privileged. If you are not the intended >> recipient please accept my apologies; please do not disclose, copy or >> distribute information in this e-mail or take any action in reliance on > its >> contents: to do so is strictly prohibited and may be unlawful. Please > inform >> me that this message has gone astray before deleting it. Thank you for > your >> co-operation >> >> >> >> >> >> >> >> ------------------------------ >> >> Message: 19 >> Date: Wed, 24 May 2006 08:09:43 +0100 >> From: Kemlo Rogerson >> Subject: RE: [Histonet] Formalin on skin >> To: "'gu.lang@gmx.at'" , "Histonetliste >> (Histonetliste)" >> Message-ID: >> >> Content-Type: text/plain >> >> Maybe it's not the physical drenching of the skin and the effects on that >> you should worry about but the fact that formalin is now on a larger warm >> surface area and the known dangers of formalin inhalation on the lung; > best >> not to experiment on Staff IMHO. >> >> Kemlo Rogerson >> Pathology Manager >> Ext 3311 >> DD 01934 647057 >> Mob 07749 754194 >> >> This e-mail is confidential and privileged. If you are not the intended >> recipient please accept my apologies; please do not disclose, copy or >> distribute information in this e-mail or take any action in reliance on > its >> contents: to do so is strictly prohibited and may be unlawful. Please > inform >> me that this message has gone astray before deleting it. Thank you for > your >> co-operation >> >> >> >> >> >> >> >> ------------------------------ >> >> Message: 20 >> Date: Wed, 24 May 2006 11:39:19 +0200 >> From: Melanie Black >> Subject: [Histonet] RE: Hoechst staining method >> To: histonet@lists.utsouthwestern.edu >> Message-ID: >> Content-Type: text/plain; charset="us-ascii" ; format="flowed" >> >> Hi Tracey >> >> Some years ago, I looked into fluorescent nuclear counterstains, >> including dapi, propidium iodide and HOECHST. Heochst is also known >> as bisBenzimide. >> >> Method: >> Mix 1mg bisBemzimide (Sigma B2883) with 1ml Dist water. Take 4.0 >> micro litres of this stock and dilute with 1ml PBS pH 7.4.Apply to >> sections for 10 mins, wash 3 x in PBS. Fluorescence is bright blue, >> viewed with a UV filter 340 nm. >> >> Stain reference: >> Latt,SA and Stetten GJ. Histochem. Cytochem 24,24 (1976) >> Araki, T et al, Histochem. 87,331 (1987) >> >> Regards >> Melanie Black >> >> >> >> >> >> >> Hi, >> We are doing an immunofluorescence research project for podocytes. >> The urine cytospins are to be counterstained with Hoescht. We have >> never used it - can someone recommend a concentration, diluent and >> staining time that we could start with. Thanks in advance. >> >> Tracey >> >> -- >> Cardiovascular Research Unit >> Div. of Cardiothoracic Surgery >> Chris Barnard Building >> University of Cape Town >> Anzio Road >> Observatory >> 7925 >> Republic of South Africa >> >> Tel +27 21 406-6589 >> Cel +27 82 469-3352 >> Fax +27 21 448-5935 >> >> >> >> ------------------------------ >> >> Message: 21 >> Date: Wed, 24 May 2006 07:17:18 -0400 >> From: "Weems, Joyce" >> Subject: RE: [Histonet] Re: Fixatives >> To: "Katri Tuomala" , >> >> Message-ID: >> <1CD6831EB9B26D45B0A3EAA79F7EBD320263E103@sjhaexc02.sjha.org> >> Content-Type: text/plain; charset="utf-8" >> >> "Surely the bottle, that the specimen comes in, is labelled with what ever >> the fixative is, along with the pertinent patient information. It should > be >> just as simple to say " specimen received in formalin (Bouins, Zenkers >> etc.)". There is no need and it is not advisable to smell it." >> >> If this statement were true, there would be no question. However, not >> everyone uses prefilled containers, and the labeling is not always > ideal. I'm >> sure this is the situation to which Dr. Bob refers...j >> >> Joyce Weems >> Pathology Manager >> Saint Joseph's Hospital of Atlanta >> 404-851-7376 >> 404-851-7831 - Fax >> >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu on behalf of Katri Tuomala >> Sent: Tue 5/23/2006 11:01 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] Re: Fixatives >> >> I have always read with respect Dr. Richmond's opinions, but here I have > to >> disagree. >> >> Surely the bottle, that the specimen comes in, is labelled with what ever >> the fixative is, along with the pertinent patient information. It should > be >> just as simple to say " specimen received in formalin (Bouins, Zenkers >> etc.)". There is no need and it is not advisable to smell it. >> >> It is an interesting mindset among many, not all, pathologist to insist in >> processing all tissues the same day (TAT!) ending up with inadequately > fixed >> and consequently poorly processed tissue, which we technologist then have > to >> struggle to produce well stained, wrinkle free, representative sections. > As >> an immuno tech I can testify for even bigger problems in producing > reliable >> results with immunohistochemical procedures. >> >> For purposes of immunostaining, it is important to know the time of > fixation >> in formalin, adequate or not (24 hours is considered adequate, not 4 or > even >> 8), so that we know what we are faced with and sometimes method can be >> adjusted to fit the fixation time. I would prefer doing immuno stains on a >> specimen, that is "overfixed" in formalin rather than underfixed. >> >> If I ever ended up with breast cancer, I would make sure, that at least > one >> tumor section got fixed 24 hours. >> >> This is my Tuesday Rant! >> >> Katri >> Katri Tuomala >> Hamilton, Ontario, Canada >> >> >> >> >> ----- Original Message ----- >> From: >> To: >> Sent: Tuesday, May 23, 2006 4:22 AM >> Subject: [Histonet] Re: Fixatives >> >> >>> Patti Loykasek at PhenoPath Laboratories notes >>We are a reference lab >>> and >>> receive specimens from all over the USA. One of my "pet peeves" is that > it >>> is >>> rare to see in the report exactly what type of fixative the specimen was >>> received in or subsequently processed in. I know we have no standard > form >>> of >>> reporting, but it just seems like best practice to me to include this >>> information on >>> the report. One of my favorites is "...received in fixative..." - not > very >>> helpful.<< >>> >>> That's exactly the phrase I use in my gross descriptions, and for a very >>> good >>> reason. I'm not about to stick my nose into every specimen bottle to >>> verify >>> that it contains formalin and not alcohol, water, or pine-scented floor >>> disinfectant (used at one hospital I know as a "fixative" for > placentas). >>> I'm willing >>> to smell-test a very occasional container where I'm suspicious that the >>> wrong >>> fixative has been used, but not every time! >>> >>> Time of fixation is the dead horse in the middle of the living room >>> floor - >>> nobody wants to hear that the HER2 immunostain for breast cancer > requires >>> overnight fixation, for example. >>> >>> Bob Richmond >>> Gastonia NC >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> Confidentiality Notice ** The information contained in this message may be > privileged and is confidential information intended for the use of the > addressee listed above. If you are neither the intended recipient nor the > employee or agent responsible for delivering this message to the intended > recipient, you are hereby notified that any disclosure, copying, > distribution or the taking of any action in reliance on the contents of this > information is strictly prohibited. If you have received this communication > in error, please notify us immediately by replying to the message and > deleting it from your computer. Thank you. Saint Joseph's Health System, > Inc. >> >> ------------------------------ >> >> Message: 22 >> Date: Wed, 24 May 2006 08:20:53 -0400 >> From: Denise Crowley >> Subject: [Histonet] cytochrome c oxidase and succinate dehydrogenase >> To: histonet@lists.utsouthwestern.edu >> Message-ID: <5CE60D25-D94D-4942-8080-49E931647809@mit.edu> >> Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed >> >> I have been asked to perform these enzyme stains on murine heart. >> Since this is new to me, I'm asking the experts for any favorite >> protocols. Even better, any commercially available kits. >> >> I also have a question about the frozen sectioning. In the clinical >> setting, we always froze the muscle biopsy without OCT. Is there a >> good reason for this? I think that filling the heart with diluted >> OCT will produce a nicer looking section, but I don't want to >> compromise the staining. >> >> Denise Crowley >> Facility Manager Histology >> Center for Cancer Research >> Massachusetts Institute of Technology >> 40 Ames St. E17-230 >> Cambridge MA 02139 >> 617-258-8183 >> dencrowl@mit.edu >> >> >> >> >> >> >> ------------------------------ >> >> Message: 23 >> Date: Wed, 24 May 2006 08:36:22 -0500 >> From: "Horn, Hazel V" >> Subject: RE: [Histonet] Sealing Paraffin Blocks >> To: kerry.l.crabb@gsk.com, histonet@lists.utsouthwestern.edu >> Message-ID: >> <9AE8AA9E1F644B4AA6C155FB6FD51C63038BE0A3@EMAIL.archildrens.org> >> Content-Type: text/plain; charset=us-ascii >> >> We used to do this years ago. We no longer seal blocks. In fact, I >> believe you cut away more on a sealed block if the block requires >> recutting. >> >> Hazel Horn >> Hazel Horn, HT/HTL (ASCP) >> Supervisor of Histology >> Arkansas Children's Hospital >> 800 Marshall Slot 820 >> Little Rock, AR 72202 >> >> phone 501.364.4240 >> fax 501.364.3912 >> >> visit us on the web at: www.archildrens.org >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >> kerry.l.crabb@gsk.com >> Sent: Tuesday, May 23, 2006 5:45 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] Sealing Paraffin Blocks >> >> What is the current opinion and practice on sealing paraffin blocks >> prior >> to filing/archiving them? What is the pro and con in your opinion? >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> -------------------------------------------------------------------------- > ---- >> The information contained in this message may be privileged and > confidential and protected from disclosure. If the reader of this message is > not the intended recipient, or an employee or agent responsible for > delivering this message to the intended recipient, you are hereby notified > that any dissemination, distribution or copying of this communication is > strictly prohibited. If you have received this communication in error, > please notify us immediately by replying to the message and deleting it from > your computer. Thank you. >> > ============================================================================ > == >> >> >> >> >> ------------------------------ >> >> Message: 24 >> Date: Wed, 24 May 2006 08:40:38 -0500 >> From: "Horn, Hazel V" >> Subject: RE: [Histonet] Re: Fixatives >> To: "Weems, Joyce" , "Katri Tuomala" >> , histonet@lists.utsouthwestern.edu >> Message-ID: >> <9AE8AA9E1F644B4AA6C155FB6FD51C63038BE0A4@EMAIL.archildrens.org> >> Content-Type: text/plain; charset=us-ascii >> >> And not all bottles say exactly what the formalin fixative is. Some >> labels are just a formalin warning label. Our fixative is Zinc buffered >> formalin. Most would probably assume the label was for 10% NBF when in >> fact it is not. We use ARUP Labs for a few tests we do not do and >> their requisition asks what fixative was used. >> >> Hazel Horn >> Hazel Horn, HT/HTL (ASCP) >> Supervisor of Histology >> Arkansas Children's Hospital >> 800 Marshall Slot 820 >> Little Rock, AR 72202 >> >> phone 501.364.4240 >> fax 501.364.3912 >> >> visit us on the web at: www.archildrens.org >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, >> Joyce >> Sent: Wednesday, May 24, 2006 6:17 AM >> To: Katri Tuomala; histonet@lists.utsouthwestern.edu >> Subject: RE: [Histonet] Re: Fixatives >> >> "Surely the bottle, that the specimen comes in, is labelled with what >> ever >> the fixative is, along with the pertinent patient information. It should >> be >> just as simple to say " specimen received in formalin (Bouins, Zenkers >> etc.)". There is no need and it is not advisable to smell it." >> >> If this statement were true, there would be no question. However, >> not >> everyone uses prefilled containers, and the labeling is not always >> ideal. I'm >> sure this is the situation to which Dr. Bob refers...j >> >> Joyce Weems >> Pathology Manager >> Saint Joseph's Hospital of Atlanta >> 404-851-7376 >> 404-851-7831 - Fax >> >> >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu on behalf of Katri >> Tuomala >> Sent: Tue 5/23/2006 11:01 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] Re: Fixatives >> >> I have always read with respect Dr. Richmond's opinions, but here I have >> to >> disagree. >> >> Surely the bottle, that the specimen comes in, is labelled with what >> ever >> the fixative is, along with the pertinent patient information. It should >> be >> just as simple to say " specimen received in formalin (Bouins, Zenkers >> etc.)". There is no need and it is not advisable to smell it. >> >> It is an interesting mindset among many, not all, pathologist to insist >> in >> processing all tissues the same day (TAT!) ending up with inadequately >> fixed >> and consequently poorly processed tissue, which we technologist then >> have to >> struggle to produce well stained, wrinkle free, representative sections. >> As >> an immuno tech I can testify for even bigger problems in producing >> reliable >> results with immunohistochemical procedures. >> >> For purposes of immunostaining, it is important to know the time of >> fixation >> in formalin, adequate or not (24 hours is considered adequate, not 4 or >> even >> 8), so that we know what we are faced with and sometimes method can be >> adjusted to fit the fixation time. I would prefer doing immuno stains on >> a >> specimen, that is "overfixed" in formalin rather than underfixed. >> >> If I ever ended up with breast cancer, I would make sure, that at least >> one >> tumor section got fixed 24 hours. >> >> This is my Tuesday Rant! >> >> Katri >> Katri Tuomala >> Hamilton, Ontario, Canada >> >> >> >> >> ----- Original Message ----- >> From: >> To: >> Sent: Tuesday, May 23, 2006 4:22 AM >> Subject: [Histonet] Re: Fixatives >> >> >>> Patti Loykasek at PhenoPath Laboratories notes >>We are a reference >> lab >>> and >>> receive specimens from all over the USA. One of my "pet peeves" is >> that it >>> is >>> rare to see in the report exactly what type of fixative the specimen >> was >>> received in or subsequently processed in. I know we have no standard >> form >>> of >>> reporting, but it just seems like best practice to me to include this >>> information on >>> the report. One of my favorites is "...received in fixative..." - not >> very >>> helpful.<< >>> >>> That's exactly the phrase I use in my gross descriptions, and for a >> very >>> good >>> reason. I'm not about to stick my nose into every specimen bottle to >>> verify >>> that it contains formalin and not alcohol, water, or pine-scented >> floor >>> disinfectant (used at one hospital I know as a "fixative" for >> placentas). >>> I'm willing >>> to smell-test a very occasional container where I'm suspicious that >> the >>> wrong >>> fixative has been used, but not every time! >>> >>> Time of fixation is the dead horse in the middle of the living room >>> floor - >>> nobody wants to hear that the HER2 immunostain for breast cancer >> requires >>> overnight fixation, for example. >>> >>> Bob Richmond >>> Gastonia NC >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> Confidentiality Notice ** The information contained in this message may >> be privileged and is confidential information intended for the use of >> the addressee listed above. If you are neither the intended recipient >> nor the employee or agent responsible for delivering this message to the >> intended recipient, you are hereby notified that any disclosure, >> copying, distribution or the taking of any action in reliance on the >> contents of this information is strictly prohibited. If you have >> received this communication in error, please notify us immediately by >> replying to the message and deleting it from your computer. Thank you. >> Saint Joseph's Health System, Inc. >> >> -------------------------------------------------------------------------- > ---- >> The information contained in this message may be privileged and > confidential and protected from disclosure. If the reader of this message is > not the intended recipient, or an employee or agent responsible for > delivering this message to the intended recipient, you are hereby notified > that any dissemination, distribution or copying of this communication is > strictly prohibited. If you have received this communication in error, > please notify us immediately by replying to the message and deleting it from > your computer. Thank you. >> > ============================================================================ > == >> >> >> >> >> ------------------------------ >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> End of Histonet Digest, Vol 30, Issue 35 >> **************************************** >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From cgfields <@t> lexhealth.org Wed May 24 12:23:21 2006 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Wed May 24 12:19:12 2006 Subject: [Histonet] FW: Aligner Message-ID: -----Original Message----- From: Carole Fields Sent: Wednesday, May 24, 2006 1:22 PM To: 'Peterson, Dan' Subject: RE: Aligner The ring is called an.... ..... XY Orientation Fork... part# 715-200...$93.95...The Richard-Allan person in your area can order them. I have all Microm microtomes. Works great and no more recut problems.. This has eliminated the problems of everyone's microtome not being lined up together. Carole Fields, HT,ASCP Pathology Supervisor Lexington Medical Center 2720 Sunset Blvd. W. Columbia, SC 29169 -----Original Message----- From: Peterson, Dan [mailto:1dpeterson@meriter.com] Sent: Wednesday, May 24, 2006 1:12 PM To: Carole Fields Subject: Aligner Carole, I just read your reply concerning blocks and you said that you use a ring behind the block holder to keep the chucks aligned. I've got one of those expensive aligners you referred to (on loan) and if there is a better alternative (and cheaper) I'd be very appreciative to find out what it's called. We use Microm microtomes here. Dan Daniel R Peterson HT(ASCP) Histopathology Section Head General Medical Laboratories (608)-267-6557 1dpeterson@meriter.com CONFIDENTIALITY NOTICE: This message (including any attachments) is intended for the sole use of the individual and entity to whom it is addressed. This message may contain information that is confidential and is protected by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply email and then delete the message. Thank you. _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. From cwscouten <@t> myneurolab.com Wed May 24 12:19:00 2006 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Wed May 24 12:19:22 2006 Subject: [Histonet] Sterotaxic instrument drawings Message-ID: <5784D843593D874C93E9BADCB87342AB01306FE5@tpiserver03.Coretech-holdings.com> We are one of three manufacturers of this equipment, no need to make your own. We are sole source for such instruments including fine drive and angle measurements and calcualtions. If you need a custom instrument, we can negotiate making it, or sell you parts. Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of nyilmaz@mersin.edu.tr Sent: Wednesday, May 24, 2006 11:53 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sterotaxic instrument drawings Dear colleagues... We're plannig to build a sterotaxic instrument for animal brain studies. It must be adaptable for mouse, rat and rabbit. We're looking for plans, drawings etc. anything beneficial. Does anybody know this kind of materials reachable on internet or another source? Thanks in advance... Dr. Necat Yilmaz Mersin University School of Medicine Histology and Embryology Dept. Mersin/TURKEY _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Tracey.Lenek <@t> CLS.ab.ca Wed May 24 12:26:59 2006 From: Tracey.Lenek <@t> CLS.ab.ca (Tracey.Lenek@CLS.ab.ca) Date: Wed May 24 12:27:38 2006 Subject: [Histonet] Stereo microscope Message-ID: <4BC300747AF87A48BCDF8E48BC2885CE02C1D7E9@mail1.calgary.com> Hi, Is anyone using Leica's EZ4 stereo mic for identifying glomeruli in renal biopsies. We are in the market for a new dissecting mic and were wondering if this model would meet our needs. If not, any recommendations? Thanks Tracey CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. From anh2006 <@t> med.cornell.edu Wed May 24 12:49:57 2006 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Wed May 24 12:49:07 2006 Subject: [Histonet] Sealing Paraffin Blocks In-Reply-To: References: Message-ID: I couldn't agree more. Exposed blocks can grow all sorts of things over time especially if not fully penetrated ... yuck! >I am a strong believer in sealing blocks. >While paraffin does penetrate into cells etc. it is still possible for >water (and organisms of various types) to penetrate into the block. >After all we use water to soak into some blocks to enhance cutting. >I have seen stored, unsealed blocks, with massive penetration of fungi >throughout the entire tissue. Also if unsealed blocks are stored in a >dry atmosphere they will tend to shrink. >If you are going to store blocks for any length of time I would seal >them not just dipping them into molten paraffin but melting the surface >layer. >Barry > -- From Nalini.Makhijani <@t> va.gov Wed May 24 13:05:40 2006 From: Nalini.Makhijani <@t> va.gov (Makhijani, Nalini S) Date: Wed May 24 13:07:34 2006 Subject: [Histonet] Paraformaldehyde Message-ID: <715FA772CEA81A47B1A762AB6E45123A0111B784@VHAV22MSGA3.v22.med.va.gov> Is 2 month old 4%PFA kept at room temp still good for the fixation of cells on coverslips? Thanks, Nalini From gcallis <@t> montana.edu Wed May 24 13:28:58 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed May 24 13:30:42 2006 Subject: [Histonet] Stent publication in J Histochem Cytochem for those interested In-Reply-To: References: Message-ID: <6.0.0.22.1.20060524122225.01b3d7d0@gemini.msu.montana.edu> Melanie, I just read your publication on stents processing and sectioning fom J Histochem Cytochem this past month. It is always nice to see a fellow Histonetter's good work in a major journal. For those interested title is Comparison of processing and sectioning methodologies for arteries containing metallic stents. JHC 54:673-681, 2006. Rippstein P, Black MK, et al. key words: stent, artery, restenosis, methyl methacrylate, resin and immunohistochemistry. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From Barry.R.Rittman <@t> uth.tmc.edu Wed May 24 13:30:53 2006 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Wed May 24 13:30:57 2006 Subject: [Histonet] Paraformaldehyde Message-ID: Nalini While the solution may not be good, how about fixing using vapor fixation. There should still be sufficient formaldehyde vapor to fix the cells and eliminates worrying about osmolarity etc of the solution. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Makhijani, Nalini S Sent: Wednesday, May 24, 2006 1:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraformaldehyde Is 2 month old 4%PFA kept at room temp still good for the fixation of cells on coverslips? Thanks, Nalini _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Tiffany.L.Sheffield <@t> uth.tmc.edu Wed May 24 13:39:38 2006 From: Tiffany.L.Sheffield <@t> uth.tmc.edu (Sheffield, Tiffany L) Date: Wed May 24 13:39:43 2006 Subject: [Histonet] Stent publication in J Histochem Cytochem for thoseinterested Message-ID: Good job Melanie!!! It was a great and helpful article. Thanks ************************************************** Tiffany Sheffield-Lopez,BS,HT(ASCP) Supervisor, Bone Histomorphometry & Biomaterials Lab Department of Orthopaedic Surgery The University of Texas Houston Health Science Center 6431 Fannin, Suite 6.144 MSB Houston, TX 77030 713-500-6803 Wk 713-500-0729 Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Wednesday, May 24, 2006 1:29 PM To: Melanie Black; Histonet@lists.utsouthwestern.edu Subject: [Histonet] Stent publication in J Histochem Cytochem for thoseinterested Melanie, I just read your publication on stents processing and sectioning fom J Histochem Cytochem this past month. It is always nice to see a fellow Histonetter's good work in a major journal. For those interested title is Comparison of processing and sectioning methodologies for arteries containing metallic stents. JHC 54:673-681, 2006. Rippstein P, Black MK, et al. key words: stent, artery, restenosis, methyl methacrylate, resin and immunohistochemistry. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asmith <@t> mail.barry.edu Wed May 24 14:20:29 2006 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Wed May 24 14:20:38 2006 Subject: [Histonet] Formalin on skin Message-ID: <5D2189E74151CC42BEC02906BA8996322B91EF@exchsrv01.barrynet.barry.edu> My understanding of formaldehyde exposure is that it is a lottery. Each occasional short exposure has a small but real chance of creating hypersensitivity, which will effectively exile one from the laboratory. I protect myself (gloves always, fume hood when possible) primarily to avoid the risk of having hypersensitivity end my career. Occasional short exposures probably do not cause cancer. Chronic exposure has a small but real chance of causing cancer. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Tuesday, May 23, 2006 1:32 PM To: Histonetliste (Histonetliste) Subject: [Histonet] Formalin on skin Hi, Today a labassistent asked me, what would happen to her skin, if formalin swallowed over her arm. I said, the formalin would harden the skin, but only on the surface. She should wash the arm with plenty of water, and there will reamain no negative reaction. It would not be dangerous for her health. Do you agree with me? Is there a cancer-risk after short contact with formalin? Greetings Gudrun Lang Histolab Akh Linz Austria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From gu.lang <@t> gmx.at Wed May 24 15:03:54 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed May 24 15:03:58 2006 Subject: AW: [Histonet] Formalin on skin In-Reply-To: <5D2189E74151CC42BEC02906BA8996322B91EF@exchsrv01.barrynet.barry.edu> Message-ID: <000401c67f6d$2f71ae60$eeeea8c0@SERVER01> Thanks to all, who have given helpfull comments on my question. Gudrun From lrichey <@t> u.washington.edu Wed May 24 16:25:17 2006 From: lrichey <@t> u.washington.edu (Lori Richey) Date: Wed May 24 16:25:33 2006 Subject: [Histonet] Sealing Paraffin Blocks In-Reply-To: References: Message-ID: <4474CF3D.8080202@u.washington.edu> We stopped sealing blocks a few years ago because of the time involved. The filing of the blocks was delayed because they were not sealed. We have had no problems with preservation, as long as the tissue is fixed and not under processed. They are stored off site in an archive storage facility, so I don't know of a mold or rodent problem. All old blocks that we cut are in good condition. kerry.l.crabb@gsk.com wrote: >What is the current opinion and practice on sealing paraffin blocks prior >to filing/archiving them? What is the pro and con in your opinion? >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From clarissabush <@t> sbcglobal.net Wed May 24 17:17:32 2006 From: clarissabush <@t> sbcglobal.net (CM Bush) Date: Wed May 24 17:17:37 2006 Subject: [Histonet] Histotech job in UC San Francisco research lab Message-ID: <20060524221732.14974.qmail@web80303.mail.yahoo.com> Hello. If you are interested in a full time histotech job in San Francisco, please go to the UCSF Human Resources Web site at: http://ucsfhr.ucsf.edu/jobs/ click "Search Career Listings" next click "Search Openings" in the box called "Req. number" type in requisition number 17697BR then click on "search" click on "Staff Research Associate" and you finally be at the actual job posting. We are required to have all resumes posted through the UCSF HR website. Thanks for your interest and we look forward to hearing from you. Best Regards, Clarissa From Shirley_PHUA <@t> hsa.gov.sg Thu May 25 03:43:42 2006 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Thu May 25 03:44:43 2006 Subject: [Histonet] DNA/RNA Extraction Kits for paraffinised tissues Message-ID: Many thanks to all who provided useful information and links on the DNA/RNA extraction kits. Shirley Phua Histopathology Laboratory Centre for Forensic Medicine Health Sciences Authority Singapore From Stephen.Eyres <@t> sanofi-aventis.com Thu May 25 04:09:37 2006 From: Stephen.Eyres <@t> sanofi-aventis.com (Stephen.Eyres@sanofi-aventis.com) Date: Thu May 25 04:21:19 2006 Subject: [Histonet] Formalin on skin Message-ID: Hi All, The key here is the amount of exposure over time. How many labs have a regular monitoring program which assesses formalin concentration whilst staff are working, grossing, tissue processor reagent changes, with or in an area where formalin vapours can build up, such as tissue store? Our lab has been monitored periodically over the years and the levels have always been below the limit. In such a situation, the carcinogenic aspect is not seen as a problem. Cheers Steve Bryan Llewellyn To: Histonet Sent by: cc: histonet-bounces@lists.utsouth Subject: Re: [Histonet] Formalin on skin western.edu 23/05/2006 20:12 Most of us old-timers have stuck our fingers in formalin or spilled it on ourselves on many occasions. I have never heard of anyone developing a tumour from it, though. It certainly doesn't appear to be common if it does happen. In Canada some years ago we had a government program assisting people to inject urea-formaldehyde resin into the walls of houses as a retrofit insulation system. A few years later it was decided the formalin fumes given off were carcinogenic, so we had another program to remove it. That's about the only link to tumours I have heard of. One of the pathologists I used to work with many years ago was fond of quoting a paper he had once read which compared naso-pharyngeal tumour rates among various groups. Pathologists had a lower rate than other people. He always put that down to the formalin fumes they breathed in all the time. The real identified problem with formalin and skin contact is dermatitis. It isn't universal, but some people do get it with repeated contact, and once you develop the sensitivity to it, it doesn't go away. So wear gloves. Bryan Llewellyn ----- Original Message ----- From: "Charles.Embrey" To: ; "Histonetliste (Histonetliste)" Sent: Tuesday, May 23, 2006 10:56 AM Subject: RE: [Histonet] Formalin on skin You are 100% correct. I have even reached a bare hand into formalin to pull out something before but washed with soap and water minutes after. I have not personally heard of formalin causing an increased risk of skin cancer however I am sure that there is a study somewhere that has probably associated some risk with L---O---N---G term exposure. Your labassistant should be perfectly safe. Just don't drink it and keep it out of the eyes :) Charles Embrey Jr. PA(ASCP) www.greyrealm.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Tuesday, May 23, 2006 12:32 PM To: Histonetliste (Histonetliste) Subject: [Histonet] Formalin on skin Hi, Today a labassistent asked me, what would happen to her skin, if formalin swallowed over her arm. I said, the formalin would harden the skin, but only on the surface. She should wash the arm with plenty of water, and there will reamain no negative reaction. It would not be dangerous for her health. Do you agree with me? Is there a cancer-risk after short contact with formalin? Greetings Gudrun Lang Histolab Akh Linz Austria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Le pr?sent message ainsi que ses ?ventuelles pi?ces jointes est exclusivement destin? au(x) destinataire(s), personnes physiques ou morales, qu'il d?signe. Il constitue de ce fait une correspondance ? caract?re priv? et peut contenir des informations confidentielles. Si ce message vous est parvenu par erreur, nous vous remercions d'en aviser imm?diatement l'exp?diteur par retour de ce courrier ?lectronique puis de le d?truire, ainsi que ses ?ventuellement pi?ces jointes, sans en conserver de copie. This message, including any attachment, is intended for the use of the individual or entity to which it is addressed. It is therefore to be considered as a private correspondence which may contain confidential information. If you are not the intended recipient, please advise the sender immediately by reply e.mail and delete this message and any attachment thereto without retaining a copy. ------------------------------ From Barry.R.Rittman <@t> uth.tmc.edu Thu May 25 05:57:20 2006 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Thu May 25 06:01:02 2006 Subject: [Histonet] Formalin on skin Message-ID: While we all seem to be concentrating on formalin, it should be remembered that combinations of different chemicals are often more damaging than exposure to a single chemical. It is therfore prudent to treat all chemicals with caution regradless of their known or perceived degree of possible damage and to stress this to all techs. Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Stephen.Eyres@sanofi-aventis.com Sent: Thu 5/25/2006 4:09 AM To: Histonet; Bryan Llewellyn Subject: Re: [Histonet] Formalin on skin Hi All, The key here is the amount of exposure over time. How many labs have a regular monitoring program which assesses formalin concentration whilst staff are working, grossing, tissue processor reagent changes, with or in an area where formalin vapours can build up, such as tissue store? Our lab has been monitored periodically over the years and the levels have always been below the limit. In such a situation, the carcinogenic aspect is not seen as a problem. Cheers Steve Bryan Llewellyn To: Histonet Sent by: cc: histonet-bounces@lists.utsouth Subject: Re: [Histonet] Formalin on skin western.edu 23/05/2006 20:12 Most of us old-timers have stuck our fingers in formalin or spilled it on ourselves on many occasions. I have never heard of anyone developing a tumour from it, though. It certainly doesn't appear to be common if it does happen. In Canada some years ago we had a government program assisting people to inject urea-formaldehyde resin into the walls of houses as a retrofit insulation system. A few years later it was decided the formalin fumes given off were carcinogenic, so we had another program to remove it. That's about the only link to tumours I have heard of. One of the pathologists I used to work with many years ago was fond of quoting a paper he had once read which compared naso-pharyngeal tumour rates among various groups. Pathologists had a lower rate than other people. He always put that down to the formalin fumes they breathed in all the time. The real identified problem with formalin and skin contact is dermatitis. It isn't universal, but some people do get it with repeated contact, and once you develop the sensitivity to it, it doesn't go away. So wear gloves. Bryan Llewellyn ----- Original Message ----- From: "Charles.Embrey" To: ; "Histonetliste (Histonetliste)" Sent: Tuesday, May 23, 2006 10:56 AM Subject: RE: [Histonet] Formalin on skin You are 100% correct. I have even reached a bare hand into formalin to pull out something before but washed with soap and water minutes after. I have not personally heard of formalin causing an increased risk of skin cancer however I am sure that there is a study somewhere that has probably associated some risk with L---O---N---G term exposure. Your labassistant should be perfectly safe. Just don't drink it and keep it out of the eyes :) Charles Embrey Jr. PA(ASCP) www.greyrealm.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Tuesday, May 23, 2006 12:32 PM To: Histonetliste (Histonetliste) Subject: [Histonet] Formalin on skin Hi, Today a labassistent asked me, what would happen to her skin, if formalin swallowed over her arm. I said, the formalin would harden the skin, but only on the surface. She should wash the arm with plenty of water, and there will reamain no negative reaction. It would not be dangerous for her health. Do you agree with me? Is there a cancer-risk after short contact with formalin? Greetings Gudrun Lang Histolab Akh Linz Austria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Le pr?sent message ainsi que ses ?ventuelles pi?ces jointes est exclusivement destin? au(x) destinataire(s), personnes physiques ou morales, qu'il d?signe. Il constitue de ce fait une correspondance ? caract?re priv? et peut contenir des informations confidentielles. Si ce message vous est parvenu par erreur, nous vous remercions d'en aviser imm?diatement l'exp?diteur par retour de ce courrier ?lectronique puis de le d?truire, ainsi que ses ?ventuellement pi?ces jointes, sans en conserver de copie. This message, including any attachment, is intended for the use of the individual or entity to which it is addressed. It is therefore to be considered as a private correspondence which may contain confidential information. If you are not the intended recipient, please advise the sender immediately by reply e.mail and delete this message and any attachment thereto without retaining a copy. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From john_paul1959 <@t> yahoo.com Thu May 25 06:04:25 2006 From: john_paul1959 <@t> yahoo.com (John Paul) Date: Thu May 25 06:04:29 2006 Subject: [Histonet] EM specimen processing pricing Message-ID: <20060525110425.37959.qmail@web31309.mail.mud.yahoo.com> Hi I was wondering if the group with help me the customary pricing for the following services 1. Tissue processing and embedding of specimen in epoxy resin 2. Cost of cutting semi thin section per slide (15 slides) stained with touludine blue Thanks John --------------------------------- Blab-away for as little as 1?/min. Make PC-to-Phone Calls using Yahoo! Messenger with Voice. From ree3 <@t> leicester.ac.uk Thu May 25 06:09:35 2006 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Thu May 25 06:09:43 2006 Subject: [Histonet] specimen size for IHC Message-ID: 10 responses in all to the section thickness question for immunostaining of paraffin sections , 2 labs cut at 3u; 1 lab cuts at 3-4u; 1 lab cuts at 4u; 4 labs cut at 5u; and 2 labs depend on what antigen is being looked for, so as ever in the wonderful world of histology, you pays your money and makes your choice!!. From Janet.Bonner <@t> FLHosp.org Thu May 25 06:12:07 2006 From: Janet.Bonner <@t> FLHosp.org (Bonner, Janet) Date: Thu May 25 06:13:45 2006 Subject: [Histonet] EM specimen processing pricing References: <20060525110425.37959.qmail@web31309.mail.mud.yahoo.com> Message-ID: Take a look at the website : http://www.mcg.edu/Core/Labs/em/feesnadprocedures.htm This should be helpful - Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of John Paul Sent: Thu 5/25/2006 7:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] EM specimen processing pricing Hi I was wondering if the group with help me the customary pricing for the following services 1. Tissue processing and embedding of specimen in epoxy resin 2. Cost of cutting semi thin section per slide (15 slides) stained with touludine blue Thanks John --------------------------------- Blab-away for as little as 1?/min. Make PC-to-Phone Calls using Yahoo! Messenger with Voice. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Michael.Rice <@t> holy-cross.com Thu May 25 07:11:18 2006 From: Michael.Rice <@t> holy-cross.com (Rice, Michael) Date: Thu May 25 07:11:35 2006 Subject: [Histonet] employment opportunity Message-ID: <3BC92F29BE821745AB15E04C98EE028D6D3939@HCH2KMAIL.holy-cross.com> Hi All, I am searching for a full time histotechnologist for our hospital in Ft Lauderdale (no alligators in the neighborhood). You must have ASCP and be eligible for a Florida license. There is no state exam. We do about 10,000 surgical cases and are automated including Dako Autostainer. We have 4 really great pathologists. It is a very low stress lab. We work M-S with once every 3 week sat rotation. Benefits are excellent and the beach is 10 minutes away and the nearest alligators for those who want to visit them are about a half hour away. Give me a call at 954.776.3070 or e-mail at Michael.rice@holy-cross.com Michael Rice CT.HT(ASCP) Supervisor Of Pathology Holy Cross Hospital Ft Lauderdale, Fl 33308 954.776.3070 ====================== Confidentiality Notice: Prepared in Anticipation of Litigation. Attorney-Client Privileged. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original. ====================== From rjbuesa <@t> yahoo.com Thu May 25 07:25:09 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu May 25 07:25:14 2006 Subject: [Histonet] EM specimen processing pricing In-Reply-To: <20060525110425.37959.qmail@web31309.mail.mud.yahoo.com> Message-ID: <20060525122509.61790.qmail@web61218.mail.yahoo.com> John: I am going to e-mail you a costs summary for histology procedures that include TEM, but as a whole not step by step. That you could calculate from the time it takes for each, materials needed, and salary component. The overall cost for TEM procedures (at 13.2 h total) is $22.10 for materials, $330.00 salary component for a total of $352.10 The average TAT for the whole is 13.2 hours as an average from 17 laboratories. You could also find some data of TEM TAT at http://www.adasp.org Ren? J. John Paul wrote: Hi I was wondering if the group with help me the customary pricing for the following services 1. Tissue processing and embedding of specimen in epoxy resin 2. Cost of cutting semi thin section per slide (15 slides) stained with touludine blue Thanks John --------------------------------- Blab-away for as little as 1?/min. Make PC-to-Phone Calls using Yahoo! Messenger with Voice. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. PC-to-Phone calls for ridiculously low rates. From funderwood <@t> mcohio.org Thu May 25 08:15:11 2006 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Thu May 25 08:15:39 2006 Subject: [Histonet] Formalin on skin Message-ID: Formaldehyde in beer. Huh. Gives new meaning to getting a "fix". >>> "Jackie M O'Connor" 05/23/06 04:32PM >>> Formaldehyde was a common ingredient of shampoos, and I remember reading the ingredients (with horror) on a bottle of Mr. Bubble bubble bath for kids (like 25 years ago). If you remember "Good Morning Vietnam" - they mentioned using formaldehyde to produce a better head on a glass of beer from the tap. I once worked with a pathologist who was allergic to formaldehyde. When he did the gross, we had to rinse all the specimens in H20 before he could examine them - yeah, that was fun. Jackie O' "Chris Pomajzl" Sent by: histonet-bounces@lists.utsouthwestern.edu 05/23/2006 01:51 PM To "HISTONET" cc Subject Re: [Histonet] Formalin on skin I would imagine that if someone had open wounds or breaks in the skin, there could be acute problems. But chronic long-term problems are unlikely with such a brief incident. People drink formaldehyde (or at least used to, wasn't it a preservative in beer many years ago??), they smoke it (I've heard of people dipping joints in formaldehyde for a stronger buzz, never tried it myself), and we breathe it in the lab every day. I believe that the carcinogenic effects are due to long-term exposure in laboratory animals. I am not aware of any studies involving human subjects. Hope this helps. ----- Original Message ----- From: "Charles.Embrey" To: ; "Histonetliste (Histonetliste)" Sent: Tuesday, May 23, 2006 12:56 PM Subject: RE: [Histonet] Formalin on skin You are 100% correct. I have even reached a bare hand into formalin to pull out something before but washed with soap and water minutes after. I have not personally heard of formalin causing an increased risk of skin cancer however I am sure that there is a study somewhere that has probably associated some risk with L---O---N---G term exposure. Your labassistant should be perfectly safe. Just don't drink it and keep it out of the eyes :) Charles Embrey Jr. PA(ASCP) www.greyrealm.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Tuesday, May 23, 2006 12:32 PM To: Histonetliste (Histonetliste) Subject: [Histonet] Formalin on skin Hi, Today a labassistent asked me, what would happen to her skin, if formalin swallowed over her arm. I said, the formalin would harden the skin, but only on the surface. She should wash the arm with plenty of water, and there will reamain no negative reaction. It would not be dangerous for her health. Do you agree with me? Is there a cancer-risk after short contact with formalin? Greetings Gudrun Lang Histolab Akh Linz Austria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rgrow <@t> bmnet.com Thu May 25 10:05:26 2006 From: rgrow <@t> bmnet.com (rgrow@bmnet.com) Date: Thu May 25 10:05:32 2006 Subject: [Histonet] Re:embedding centers In-Reply-To: Message-ID: Deanna RUN from Microm embedding centers. Microm makes great microtomes but not embedding centers. We had an AP280, purchased in '99 that was a constant lemon. Paraffin chamber and cold plate had continuous problems. It was sent back numerous times and even replaced but still would not work properly. Contact me for details. By 2002, we trashed it and replaced it with a new Tissue Tek Tec 5 Embedding Station. We now have 2 in the lab. Tissue Tec is the best workhorse out there. I have used it for years prior and am glad to have it in this laboratory. Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax Message: 10 Date: Wed, 24 May 2006 11:24:18 -0400 From: "Snider, Deanna" Subject: [Histonet] embedding centers To: Message-ID: <84BE46B37B314D409C5A17B7BAB022D68BC9CF@IDC-EX-VS01.shriners.cc> Content-Type: text/plain; charset="us-ascii" Hello all! I need your masses of expertise yet again!! I thank you in advance. My lab is in the process of acquiring a new embedding center. What are your experiences with the various embedding centers out there? Why do you like/dislike about the unit? How is your experience with the customer support? I am considering the Microm ec350, so any info on this particular unit is greatly appreciated. Thanks so much again! Deanna Snider HT ASCP Lead Histotechnician, Research Department Shriners Hospital for Children Cincinnati, OH 45229 513-872-6388 From tkngflght <@t> yahoo.com Thu May 25 10:57:51 2006 From: tkngflght <@t> yahoo.com (Cheryl) Date: Thu May 25 10:57:56 2006 Subject: [Histonet] Looking for new histo grads!! In-Reply-To: <3BC92F29BE821745AB15E04C98EE028D6D3939@HCH2KMAIL.holy-cross.com> Message-ID: <20060525155751.7066.qmail@web50907.mail.yahoo.com> Hello everyone-- I post under my personal email (tkngflght@yahoo.com) and wear a histologist hat (the one with paraffin and heme all over it)...this is posted in my role as a histology recruiter: I have a great opportunity for new histology graduates. Of course I'm alway open to help you veterans, but this is a new development for those just out of school looking for a great job with excellent growth potential. I'm VERY careful with where I will place a new grad--we all want you to learn what you need to know on top of your schooling and to LIKE histology so you STAY in the field!! These jobs are in those very places. Give me a call, or send your resume (not to the list serve, please). admin@fullstaff.org I am a histotech--have been one for over 20 years and I still work the bench. Full Staff is the company name--our mission is to help histologists find a lab home that will help them grow and meet their professional goals in balance their personal lives--not to fill a list of open jobs. References from techs I've helped in the past are available. I'll share more about the jobs and how we can help when you call. No obligation and never any cost to you-- Cheryl Kerry, HT(ASCP) Full Staff Inc Helping staff the AP Lab - Helping on Tech at a time admin@fullstaff.org 800.756.3309 phone and fax 281.852.9457 office From sjchtascp <@t> yahoo.com Thu May 25 11:12:22 2006 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Thu May 25 11:12:28 2006 Subject: [Histonet] hematoxylins ?? Message-ID: <20060525161222.28813.qmail@web38206.mail.mud.yahoo.com> Whats the best hematoxlyin powder/crystal to use in histology. I purchased a powder CI 175290 which contains OG powder ???. In making up weigerts Iron Hematoxylin I ended up using twice the recommended just to get the solution to stain properly. I will be making up a lead hematoxylin for pancreatic D cells and am starting to think I need a different hematoxylin. Steve --------------------------------- Blab-away for as little as 1?/min. Make PC-to-Phone Calls using Yahoo! Messenger with Voice. From Lori.Karnes <@t> saskatoonhealthregion.ca Thu May 25 11:28:10 2006 From: Lori.Karnes <@t> saskatoonhealthregion.ca (Karnes, Lori SktnHR) Date: Thu May 25 11:28:44 2006 Subject: [Histonet] Re:embedding centers Message-ID: <7A83E83CB5B81A4A83F6A8268392B0C2848567@stampy.sktnhr.ca> Ditto: we have 2 microms and they have been nothing but trouble. They have a great design but are always down. Lori Karnes ART Tech III, Histology Laboratory Saskatoon Health Region Phone: (306) 655-8197 Email: lori.karnes@saskatoonhealthregion.ca -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of rgrow@bmnet.com Sent: Thursday, May 25, 2006 9:05 AM To: histonet@lists.utsouthwestern.edu Cc: dsnider@shrinenet.org Subject: [Histonet] Re:embedding centers Deanna RUN from Microm embedding centers. Microm makes great microtomes but not embedding centers. We had an AP280, purchased in '99 that was a constant lemon. Paraffin chamber and cold plate had continuous problems. It was sent back numerous times and even replaced but still would not work properly. Contact me for details. By 2002, we trashed it and replaced it with a new Tissue Tek Tec 5 Embedding Station. We now have 2 in the lab. Tissue Tec is the best workhorse out there. I have used it for years prior and am glad to have it in this laboratory. Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax Message: 10 Date: Wed, 24 May 2006 11:24:18 -0400 From: "Snider, Deanna" Subject: [Histonet] embedding centers To: Message-ID: <84BE46B37B314D409C5A17B7BAB022D68BC9CF@IDC-EX-VS01.shriners.cc> Content-Type: text/plain; charset="us-ascii" Hello all! I need your masses of expertise yet again!! I thank you in advance. My lab is in the process of acquiring a new embedding center. What are your experiences with the various embedding centers out there? Why do you like/dislike about the unit? How is your experience with the customer support? I am considering the Microm ec350, so any info on this particular unit is greatly appreciated. Thanks so much again! Deanna Snider HT ASCP Lead Histotechnician, Research Department Shriners Hospital for Children Cincinnati, OH 45229 513-872-6388 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From caron_fournier <@t> yahoo.ca Thu May 25 11:34:45 2006 From: caron_fournier <@t> yahoo.ca (caron fournier) Date: Thu May 25 11:34:49 2006 Subject: [Histonet] re: spurr embedde sheep spine Message-ID: <20060525163445.50317.qmail@web35413.mail.mud.yahoo.com> Hi All: I have some sheep spine that have been processed and embedded in Spurr resin but the spurr has remains "sticky" in the middle of the block near the spine. I was wondering if anyone has a suggestion about what I can do to get this to harden enough that I can section them with our bandsaw and grind them. We have had them curing in a 60 degree oven for a couple of weeks and they have gotten a little better but not completely hard. Any suggestions would be appreciated. Caron --------------------------------- Share your photos with the people who matter at Yahoo! Canada Photos From Amanda.Garcia <@t> TriadHospitals.com Thu May 25 13:26:34 2006 From: Amanda.Garcia <@t> TriadHospitals.com (Garcia, Amanda) Date: Thu May 25 13:26:40 2006 Subject: [Histonet] (no subject) Message-ID: <8B63039C9DF4554C8FDBF31235F0E148016DEB87@CPRTEVS02.triadhospitals.net> Can anyone help me out and tell me where you order diastase? Thanks in advance. > Amanda (Amy) Garcia > Histology/Pathology > College Station Medical Center > (979) 680-5372 office > (979) 696-5446 fax > *Mailto:amanda.garcia@triadhospitals.com > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the e-mail or any > of its attachments, please be advised that you have received this > e-mail in error and that any use, dissemination, distribution, > forwarding, printing, or copying of this e-mail or any attached files > is strictly prohibited. If you have received this e-mail in error, > please immediately purge it and all attachments and notify the sender > by reply e-mail or contact the sender at the number listed. > > > From SHARON.OSBORN <@t> SPCORP.COM Thu May 25 13:30:55 2006 From: SHARON.OSBORN <@t> SPCORP.COM (Osborn, Sharon) Date: Thu May 25 13:32:00 2006 Subject: [Histonet] formaldehyde in beer, etc. Message-ID: <9A919A5D70313A4D9C56A025710874080C72C1@kenmsg40.us.schp.com> Yes, in the past, formaldehyde has been added to the beer to ship to the military and may still be done. It is used as a preservative. Thus, the term "rot gut" for when the guys get sick with upset stomachs, numb tongues, and headaches, etc.; it is not all from the alcohol! (this from some who served and drank!). And, I remember the first time my Dad walked into the histology lab I was working in back in the late 1960's, early 1970's. The pathologist was grossing in with the open container of formalin and tissue on the board. Of course, back then, no gloves; the hands went into the formalin to p/u the tissue, etc. The first comment from my Dad was "that's 'maldehyde you are using!" I had to ask him several times before understanding he was talking about formaldehyde. He kept saying he smelled it in the packin' plant. He was familiar with it in the butchering plants/packing plants for cattle, hogs, etc. We had a farm and he would take some animals for slaughter for his custom delivery of beef or hogs to customers. He said they sprayed it in the cooling out rooms to keep the meat from spoiling. So, even your food often has it as a preservative on it. Then, have you ever walked into a fabric store and your eyes start burning, watering, etc. and nose also? It is the strong odor of formaldehyde in the fabrics. It is used in the fabrics our clothing is made of; in the carpet in our homes; in the glues used for the carpets and the furniture; in the chipped wood products that have laminates over them, etc. Then, with our homes tightly sealed when all doors and windows are shut, these gases have no way to escape and we breathe more of it than otherwise. These are current ways I know formaldehyde is being used plus the shampoos already mention. Look at your labels, some hand lotions have it in it also! sharon osborn DNAX, SP BioPharma Palo Alto, CA Date: Thu, 25 May 2006 09:15:11 -0400 From: "Fred Underwood" Subject: Re: [Histonet] Formalin on skin To: , Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Formaldehyde in beer. Huh. Gives new meaning to getting a "fix". >>> "Jackie M O'Connor" 05/23/06 04:32PM >>> Formaldehyde was a common ingredient of shampoos, and I remember reading the ingredients (with horror) on a bottle of Mr. Bubble bubble bath for kids (like 25 years ago). If you remember "Good Morning Vietnam" - they mentioned using formaldehyde to produce a better head on a glass of beer from the tap. I once worked with a pathologist who was allergic to formaldehyde. When he did the gross, we had to rinse all the specimens in H20 before he could examine them - yeah, that was fun. Jackie O' ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From mcauliff <@t> umdnj.edu Thu May 25 13:37:04 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu May 25 13:36:45 2006 Subject: [Histonet] re: spurr embedde sheep spine In-Reply-To: <20060525163445.50317.qmail@web35413.mail.mud.yahoo.com> References: <20060525163445.50317.qmail@web35413.mail.mud.yahoo.com> Message-ID: <4475F950.6020009@umdnj.edu> Hi Caron: Are you using the "new" Spurr in which ERL 4206 has been replaced by ERL 4221? If so, the instructions on how to mix the various components are almost certainly wrong. All of the vendors I have had experience with are sending out the wrong formulations, apparently because they have been told that the 4221 can replace 4206 on a 1:1 basis. Wrong! The result is a poorly infiltrated block. Now, if you are using an older kit with 4206: 1. your accelerator is probably old 2. mixing of components was incomplete 3. the blocks are too big to infiltrate in the time allocated. I don't think you will be able to harden the blocks you already have, sorry. Maybe you can cut the blocks, dissolve out the sticky resin and re-infiltrate with fresh. Geoff caron fournier wrote: >Hi All: > I have some sheep spine that have been processed and embedded in Spurr resin but the spurr has remains "sticky" in the middle of the block near the spine. I was wondering if anyone has a suggestion about what I can do to get this to harden enough that I can section them with our bandsaw and grind them. We have had them curing in a 60 degree oven for a couple of weeks and they have gotten a little better but not completely hard. Any suggestions would be appreciated. > Caron > > >--------------------------------- >Share your photos with the people who matter at Yahoo! Canada Photos >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From Eric.C.Kellar <@t> questdiagnostics.com Thu May 25 14:15:53 2006 From: Eric.C.Kellar <@t> questdiagnostics.com (Kellar, Eric C) Date: Thu May 25 14:16:05 2006 Subject: [Histonet] Re: Diastase of Malt Message-ID: <6843061CE6B98E4B96590D4F299618F80156663C@qdcws0117.us.qdx.com> Amy, I order mine from Fisher Scientific Diastase of Malt Laboratory Grade #D22-100 Eric C. Kellar Quest Diagnostics Inc. Miami Can anyone help me out and tell me where you order diastase? Thanks in advance. > Amanda (Amy) Garcia > Histology/Pathology > College Station Medical Center > (979) 680-5372 office > (979) 696-5446 fax > *Mailto:amanda.garcia@triadhospitals.com ============================================================================== The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. ============================================================================== From Reuel.Cornelia <@t> tsrh.org Thu May 25 15:07:46 2006 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Thu May 25 15:10:11 2006 Subject: [Histonet] Feed back on bone fixation Message-ID: We are fixing our bone with 70% alcohol for our research project, some will be process for MMA(plastic) and the rest for paraffin embedding. We will be transferring the bone tissue to 10% formalin for 24 hrs and decalcify with EDTA for HE, Enzyme and Immunocytochemistry studies for our paraffin embedded bone. We normally fixed the tissue in 10% formalin and transfer the rest of the tissue to 70% alcohol for storage. But we never have done 70% alcohol to 10% formalin in any of our paraffin embedded tissue. Any comments, theoretical explanation on the effects of fixation will be helpful. ******************************************************************************************************************* Texas Scottish Rite Hospital for Children is one of the nation's leading pediatric centers for the treatment of orthopedic conditions and learning disorders, like dyslexia. This email transmission and/or its attachments may contain confidential health information, intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing it to any other party unless required to do so by law and is required to delete/destroy the information after its stated need has been fulfilled. If you are not the intended recipient, any disclosure, copying, distribution or action taken in reliance on the contents of this email transmission is prohibited. If you have received this information in error, please notify the sender immediately and delete this information. We appreciate your efforts to protect the children's confidential information. ******************************************************************************************************************* From detmar <@t> mshri.on.ca Thu May 25 16:50:03 2006 From: detmar <@t> mshri.on.ca (Jacqui Detmar) Date: Thu May 25 16:50:10 2006 Subject: [Histonet] methyl green counterstain Message-ID: Hi all. I am doing some immunohistochemistry and TUNEL staining on mouse placental tissue. I have noticed that my methyl green counterstain on placentae assayed for TUNEL looks normal, but when I apply the same steps to tissue from the same block, but exposed to immunohistochemistry, the methyl green counterstain is very weak, bleaches out quickly during dehydration and generally looks pretty crappy. The methyl green recipe I am using is as follows: 0.5% methyl green in 0.1M sodium acetate buffer, pH 4.2. I would like to emphasize that no matter how quickly I dehydrate with the IHC sections, the colour is still very dim. The reason I am using methyl green is b/c I am doing IHC for nuclear proteins (Ki67, etc.) and don't want to use hematoxylin, since it's a little dark. Any suggestions? Thanks, Jacqui Detmar From lpwenk <@t> sbcglobal.net Thu May 25 17:07:57 2006 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu May 25 17:08:15 2006 Subject: [Histonet] hematoxylins ?? In-Reply-To: <20060525161222.28813.qmail@web38206.mail.mud.yahoo.com> Message-ID: <000d01c68047$ae101240$042f1544@HPPav2> Any hematoxylin powder without any other dye like Orange G (OG). The hematoxylin Color Index is 75290, not 175290. Fisher, Polyscience, and Sigma sell it, to name a few. I have a couple of questions, though, if I could be so bold. - how are you planning on disposing of the lead hematoxylin? It cannot be disposed of down the sink, including all the water washing after hematoxylin staining. - have you consider immunohistochemistry for somatostatin? Much more specific. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Coakley Sent: Thursday, May 25, 2006 12:12 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] hematoxylins ?? Whats the best hematoxlyin powder/crystal to use in histology. I purchased a powder CI 175290 which contains OG powder ???. In making up weigerts Iron Hematoxylin I ended up using twice the recommended just to get the solution to stain properly. I will be making up a lead hematoxylin for pancreatic D cells and am starting to think I need a different hematoxylin. Steve --------------------------------- Blab-away for as little as 1?/min. Make PC-to-Phone Calls using Yahoo! Messenger with Voice. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Thu May 25 18:01:46 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu May 25 18:02:00 2006 Subject: [Histonet] (no subject) Message-ID: Amanda, We use the following: Amylase Reagent Warning: Harmful, contains azide - see MSDS Alpha Amylase from Bacillus Subtilis (Fluka Cat No 10070,) 1g Oxoid PBS Tablets (Cat No BR14a) 1 tablet Distilled water 100ml Sodium Azide 0.1g This solution, once prepared is stored at 4oC when not in use. A recycled antibody dropper bottle (often used in commercial immunoperoxidase kits) is useful for storage and application. Reference: V-M. Mangan, V. Farago, M. Kelly, and A. F. Henwood (2002) " An Amylase Reagent with a Long Shelf Life for the Removal of Glycogen from Tissue Sections" J Histotechnol. 25(3): 153-4. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Garcia, Amanda Sent: Friday, 26 May 2006 4:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Can anyone help me out and tell me where you order diastase? Thanks in advance. > Amanda (Amy) Garcia > Histology/Pathology > College Station Medical Center > (979) 680-5372 office > (979) 696-5446 fax > *Mailto:amanda.garcia@triadhospitals.com > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the e-mail or any > of its attachments, please be advised that you have received this > e-mail in error and that any use, dissemination, distribution, > forwarding, printing, or copying of this e-mail or any attached files > is strictly prohibited. If you have received this e-mail in error, > please immediately purge it and all attachments and notify the sender > by reply e-mail or contact the sender at the number listed. > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From froyer <@t> bitstream.net Thu May 25 22:32:06 2006 From: froyer <@t> bitstream.net (froyer@bitstream.net) Date: Thu May 25 22:32:12 2006 Subject: [Histonet] Formalin on skin In-Reply-To: References: Message-ID: <1119.209.136.24.170.1148614326.squirrel@webmail.iphouse.com> I am reminded of a story that my son told me from his college days. He majored in Chemistry and went on to get a M.S. in Chemical engineering. On the first day of his second year Organic Chemistry Laboratory the T.A. was giving the class the basic ground rules and safety procedures for their laboratory sessions. The one thing that stuck in his mind is when the T.A. explained what they would be dealing with on this level. "Up until now, in your Freshman labs, and any labs that you may have had prior in High school, the chemicals that you have handled could hurt you. The chemicals that you will be dealing with throughout the course of this lab can KILL you." He never forgot that, as did all of his class mates, for the rest of the semester,and thankfully so. They are all still with us today. ~ Ford Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. Minneapolis, MN > While we all seem to be concentrating on formalin, it should be remembered > that combinations of different chemicals are often more damaging than > exposure to a single chemical. > It is therfore prudent to treat all chemicals with caution regradless of > their known or perceived degree of possible damage and to stress this to > all techs. > Barry > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > Stephen.Eyres@sanofi-aventis.com > Sent: Thu 5/25/2006 4:09 AM > To: Histonet; Bryan Llewellyn > Subject: Re: [Histonet] Formalin on skin > > > > > > > > Hi All, > > The key here is the amount of exposure over time. How many labs have a > regular monitoring program which assesses formalin concentration whilst > staff are working, grossing, tissue processor reagent changes, with or in > an area where formalin vapours can build up, such as tissue store? Our > lab > has been monitored periodically over the years and the levels have always > been below the limit. In such a situation, the carcinogenic aspect is not > seen as a problem. > > Cheers > > Steve > > > > Bryan Llewellyn > To: > Histonet > Sent by: cc: > histonet-bounces@lists.utsouth Subject: Re: > [Histonet] Formalin on skin > western.edu > > > 23/05/2006 20:12 > > > > > > Most of us old-timers have stuck our fingers in formalin or spilled it on > ourselves on many occasions. I have never heard of anyone developing a > tumour from it, though. It certainly doesn't appear to be common if it > does > happen. In Canada some years ago we had a government program assisting > people to inject urea-formaldehyde resin into the walls of houses as a > retrofit insulation system. A few years later it was decided the formalin > fumes given off were carcinogenic, so we had another program to remove it. > That's about the only link to tumours I have heard of. > > One of the pathologists I used to work with many years ago was fond of > quoting a paper he had once read which compared naso-pharyngeal tumour > rates > among various groups. Pathologists had a lower rate than other people. > He > > always put that down to the formalin fumes they breathed in all the time. > > The real identified problem with formalin and skin contact is dermatitis. > It isn't universal, but some people do get it with repeated contact, and > once you develop the sensitivity to it, it doesn't go away. So wear > gloves. > > Bryan Llewellyn > > > > ----- Original Message ----- > From: "Charles.Embrey" > To: ; "Histonetliste (Histonetliste)" > > Sent: Tuesday, May 23, 2006 10:56 AM > Subject: RE: [Histonet] Formalin on skin > > > You are 100% correct. I have even reached a bare hand into formalin to > pull out something before but washed with soap and water minutes after. > I have not personally heard of formalin causing an increased risk of > skin cancer however I am sure that there is a study somewhere that has > probably associated some risk with L---O---N---G term exposure. Your > labassistant should be perfectly safe. Just don't drink it and keep it > out of the eyes :) > > Charles Embrey Jr. PA(ASCP) > www.greyrealm.com > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gudrun > Lang > Sent: Tuesday, May 23, 2006 12:32 PM > To: Histonetliste (Histonetliste) > Subject: [Histonet] Formalin on skin > > Hi, > Today a labassistent asked me, what would happen to her skin, if > formalin > swallowed over her arm. > I said, the formalin would harden the skin, but only on the surface. She > should wash the arm with plenty of water, and there will reamain no > negative > reaction. It would not be dangerous for her health. > > Do you agree with me? Is there a cancer-risk after short contact with > formalin? > > Greetings > Gudrun Lang > > Histolab > Akh Linz > Austria > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > Le pr?sent message ainsi que ses ?ventuelles pi?ces jointes est > exclusivement destin? au(x) destinataire(s), personnes physiques ou > morales, qu'il d?signe. > Il constitue de ce fait une correspondance ? caract?re priv? et peut > contenir des informations confidentielles. > Si ce message vous est parvenu par erreur, nous vous remercions d'en > aviser > imm?diatement l'exp?diteur par retour de ce courrier ?lectronique puis de > le d?truire, ainsi que ses ?ventuellement pi?ces jointes, sans en > conserver > de copie. > > This message, including any attachment, is intended for the use of the > individual or entity to which it is addressed. > It is therefore to be considered as a private correspondence which may > contain confidential information. > If you are not the intended recipient, please advise the sender > immediately > by reply e.mail and delete this message and any attachment thereto without > retaining a copy. > ------------------------------ > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From mbmphoto <@t> gmail.com Fri May 26 00:37:11 2006 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Fri May 26 00:36:32 2006 Subject: [Histonet] looking for oligodendrocyte antibody for FS Message-ID: <2467A1A3-CBAC-4AC1-977D-B67E3B175825@gmail.com> I'm looking for a good oligodendrocyte primary antibody on Zamboni fixed monkey brain tissue. The brain tissue has been snapped frozen and sectioned at 40ums for free-floating sections. We have tried Chemicon's (MAB328) mouse anti-mylein/oligodendrocyte specific protein monoclonal primary antibody (isotype IgM) & for the secondary antibody we used rhodamine (TRITC) conjugated AP goat anti-mouse IgG +IgM cross reaction to human without success - meaning NO staining!!! If someone has any suggestions or has used another good working oligodendrocyte primary antibody - please contact me. I would be most grateful to hear from you. Yours Maria Bartola Mejia University of California San Francisco (UCSF) Department of Neurosurgery San Francisco, CA 94103 From mikael.niku <@t> helsinki.fi Fri May 26 03:11:47 2006 From: mikael.niku <@t> helsinki.fi (Mikael Niku) Date: Fri May 26 03:11:58 2006 Subject: [Histonet] Re: Autofluorescence/Imunofluorescence protocols rat kidney In-Reply-To: <4469b9af.7c.7cd1.1813511228@student.ucc.ie> References: <4469b9af.7c.7cd1.1813511228@student.ucc.ie> Message-ID: <4476B843.20104@helsinki.fi> Hello! I recently evaluated several easy methods to quench autofluoresence for paraformaldehyde-fixed paraffin sections of bovine tissues. The only thing that worked (but worked beautifully!) was Sudan Black B (CI 26150). A 5-30 min incubation in a 0.1% solution in 70% ethanol, after the whole immunofluorescence staining procedure, eliminated practically all autofluorescence in any tissue, without affecting signals from alexa488 or alexa546 too much. It's like magic! The only drawback was that it seems to quench the DAPI counterstain. Just prepare the solution carefully, it won't solubilize very quickly. And wash the slides with plenty of running water after the Sudan treatment. With best regards, Mikael PS In addition to several chemical treatments, I also tried the recently published method of irradiating the sections with fluorescent tubes of specific colors. Did not have any effect at all. Anyone know what I could have been doing wrong? -- //////////////////////////////////////////////////////////// Mikael Niku URL: www.helsinki.fi/~mniku/ University of Helsinki Dept. Basic Veterinary Sciences - Mit?k? mielt? olen l?nsimaisesta sivistyksest?? Minusta se olisi erinomainen ajatus! - Gandhi //////////////////////////////////////////////////////////// From matthew.ibbs <@t> wco.pl Fri May 26 05:23:20 2006 From: matthew.ibbs <@t> wco.pl (MRI) Date: Fri May 26 05:23:33 2006 Subject: [Histonet] Microwave processors Message-ID: <4476D718.5090002@wco.pl> Dear All, Our department has been trying to buy a microwave assissted tissue processor for about a year, but one of the major stumbling blocks in this process is that one of the pathologists in our department insists that laboratories "in the west" (we're in Poland) are abandoning these machines as they damage the blocks of tissue inside. Is there any truth in this? Are there any unhappy microwave processor users on Histonet? If so, I'd like to hear from you, explaining what the most common problems are and what you've tried to solve them. Also, I'd like to hear from people who have positive things to say about these machines. In either case, please make sure you include details of the make and model of the processor in your reply. Many thanks in advance. Matt. -- MRI WCO From jqb7 <@t> cdc.gov Fri May 26 05:29:10 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/NCID/VR)) Date: Fri May 26 05:29:18 2006 Subject: [Histonet] Microwave processors Message-ID: We have used both the Milestone TT/Mega and Sakura Xpress. Both have performed beautifully. The TT/Mega is not a walk-away machine so it requires more work to remove buckets, change, solutions, etc. The Xpress is great as you can load baskets continuously every 15 minutes and walk away. We have had no problems at all with any diagnostic cases. We have processed biopsies and larger specimens. We do not have the tissue variety of a hospital lab and therefore cannot comment on some tissues, such as breast. But we have processed most other types of tissues, including brain. Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MRI Sent: Friday, May 26, 2006 6:23 AM To: Histonet Subject: [Histonet] Microwave processors Dear All, Our department has been trying to buy a microwave assissted tissue processor for about a year, but one of the major stumbling blocks in this process is that one of the pathologists in our department insists that laboratories "in the west" (we're in Poland) are abandoning these machines as they damage the blocks of tissue inside. Is there any truth in this? Are there any unhappy microwave processor users on Histonet? If so, I'd like to hear from you, explaining what the most common problems are and what you've tried to solve them. Also, I'd like to hear from people who have positive things to say about these machines. In either case, please make sure you include details of the make and model of the processor in your reply. Many thanks in advance. Matt. -- MRI WCO _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Fri May 26 06:38:40 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri May 26 06:40:49 2006 Subject: [Histonet] (no subject) References: <8B63039C9DF4554C8FDBF31235F0E148016DEB87@CPRTEVS02.triadhospitals.net> Message-ID: We us the Porcine Amylase from Sigma cat# A3176. Works great and takes VERY little in 50cc warm H2O. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Garcia, Amanda Sent: Thu 5/25/2006 2:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Can anyone help me out and tell me where you order diastase? Thanks in advance. > Amanda (Amy) Garcia > Histology/Pathology > College Station Medical Center > (979) 680-5372 office > (979) 696-5446 fax > *Mailto:amanda.garcia@triadhospitals.com > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the e-mail or any > of its attachments, please be advised that you have received this > e-mail in error and that any use, dissemination, distribution, > forwarding, printing, or copying of this e-mail or any attached files > is strictly prohibited. If you have received this e-mail in error, > please immediately purge it and all attachments and notify the sender > by reply e-mail or contact the sender at the number listed. > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHosp.org Fri May 26 06:51:18 2006 From: Janet.Bonner <@t> FLHosp.org (Bonner, Janet) Date: Fri May 26 06:51:43 2006 Subject: [Histonet] Microwave processors References: <4476D718.5090002@wco.pl> Message-ID: I'm not aware of any discontent with the microwave processing. In our facility there is a big push to do everything microwave so that our process of getting the slides to the Pathologists is facilitated! Infiltration is better in the fatty tissue (breast), and our late machine will come off six hours sooner. The microwave procedures are also in a constant state of improvement and development. We've been researching this issue and trying to get this project off the ground for three years, but the size of the processors or cost has been a stumbling block. Damage to the blocks could have been due to a repeated technical problem and the facility the Pathologist talked to simply gave up. See what everyone else says about this - let me know if it's valid!!! Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of MRI Sent: Fri 5/26/2006 6:23 AM To: Histonet Subject: [Histonet] Microwave processors Dear All, Our department has been trying to buy a microwave assissted tissue processor for about a year, but one of the major stumbling blocks in this process is that one of the pathologists in our department insists that laboratories "in the west" (we're in Poland) are abandoning these machines as they damage the blocks of tissue inside. Is there any truth in this? Are there any unhappy microwave processor users on Histonet? If so, I'd like to hear from you, explaining what the most common problems are and what you've tried to solve them. Also, I'd like to hear from people who have positive things to say about these machines. In either case, please make sure you include details of the make and model of the processor in your reply. Many thanks in advance. Matt. -- MRI WCO _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Fri May 26 07:08:02 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/NCID/VR)) Date: Fri May 26 07:09:03 2006 Subject: [Histonet] Microwave processors Message-ID: Janet: I agree about the damage to the blocks perhaps being a technical (technician) problem. Dr. Morales at the Univ. of Miami designed the Sakura Xpress and he has said that they have tested tens of thousands of specimens and not one has been damaged. Anyone who wants details should contact him. His is a hospital facility and I am sure he would be happy to talk with anyone who calls about this concern. Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonner, Janet Sent: Friday, May 26, 2006 7:51 AM To: MRI; Histonet Subject: RE: [Histonet] Microwave processors I'm not aware of any discontent with the microwave processing. In our facility there is a big push to do everything microwave so that our process of getting the slides to the Pathologists is facilitated! Infiltration is better in the fatty tissue (breast), and our late machine will come off six hours sooner. The microwave procedures are also in a constant state of improvement and development. We've been researching this issue and trying to get this project off the ground for three years, but the size of the processors or cost has been a stumbling block. Damage to the blocks could have been due to a repeated technical problem and the facility the Pathologist talked to simply gave up. See what everyone else says about this - let me know if it's valid!!! Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of MRI Sent: Fri 5/26/2006 6:23 AM To: Histonet Subject: [Histonet] Microwave processors Dear All, Our department has been trying to buy a microwave assissted tissue processor for about a year, but one of the major stumbling blocks in this process is that one of the pathologists in our department insists that laboratories "in the west" (we're in Poland) are abandoning these machines as they damage the blocks of tissue inside. Is there any truth in this? Are there any unhappy microwave processor users on Histonet? If so, I'd like to hear from you, explaining what the most common problems are and what you've tried to solve them. Also, I'd like to hear from people who have positive things to say about these machines. In either case, please make sure you include details of the make and model of the processor in your reply. Many thanks in advance. Matt. -- MRI WCO _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DDittus787 <@t> aol.com Fri May 26 08:12:02 2006 From: DDittus787 <@t> aol.com (DDittus787@aol.com) Date: Fri May 26 08:16:01 2006 Subject: [Histonet] methyl green counterstain Message-ID: <487.f69954.31a858a2@aol.com> Jacqi: Methyl green does wash out with alcohols and if I recall correctly it did better with other types of hazardous chemicals. Have you tried a diluted fast Green counterstain? Dana Dittus Polysciences,Inc. From hymclab <@t> hyhc.com Fri May 26 08:56:50 2006 From: hymclab <@t> hyhc.com (hymclab) Date: Fri May 26 08:50:44 2006 Subject: [Histonet] (no subject) Message-ID: We get ours from Poly Scientific. Dawn -----Original Message----- From: Garcia, Amanda [mailto:Amanda.Garcia@TriadHospitals.com] Sent: Thursday, May 25, 2006 1:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Can anyone help me out and tell me where you order diastase? Thanks in advance. > Amanda (Amy) Garcia > Histology/Pathology > College Station Medical Center > (979) 680-5372 office > (979) 696-5446 fax > *Mailto:amanda.garcia@triadhospitals.com > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the e-mail or any > of its attachments, please be advised that you have received this > e-mail in error and that any use, dissemination, distribution, > forwarding, printing, or copying of this e-mail or any attached files > is strictly prohibited. If you have received this e-mail in error, > please immediately purge it and all attachments and notify the sender > by reply e-mail or contact the sender at the number listed. > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LRaff <@t> lab.uropartners.com Fri May 26 09:34:56 2006 From: LRaff <@t> lab.uropartners.com (Lester Raff) Date: Fri May 26 09:35:02 2006 Subject: [Histonet] Looking for histologists in Chicago Area Message-ID: <5DA1CA5D0B98A84985B545A24423B8220197E6@UPLAB01.uplab.local> Hi Histonetters: We would still like to pick up a histology tech to work occasional days at our west suburban Chicago private lab. We are currently only looking for day shift, Monday-Friday. If you are interested in being on our registry, please let me know. Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 From TillRenee <@t> uams.edu Fri May 26 10:23:31 2006 From: TillRenee <@t> uams.edu (Till, Renee) Date: Fri May 26 10:24:08 2006 Subject: [Histonet] whole villi Message-ID: <11F927674DEBDC43B960809A7403C5D2010CEDC7@MAILPED.ad.uams.edu> I sometimes work with rat and mouse gi tissues, and as you might expect one of the most time consuming things is getting the tissues embedded good. The colon has not been as much of a problem, but lately we are doing more with the jejunum and getting whole villi is enough to make me scream. It seems to me that one of my main problems is that sometime between the collection of the tissues (which is not done by me) and when I embed them, the tissues often curl up and it is hard to get a good straight piece to embed. We embed them on end? (so when cut it makes a little O). Any ideas on how to keep them straight during the processing? Or maybe if there is something else that I am not thinking of that could be a problem. Thanks. Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72002 Office (501)364-2785 Fax (501)364-3161 ================================================================================================ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ================================================================================================ From Barry.R.Rittman <@t> uth.tmc.edu Fri May 26 10:36:35 2006 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Fri May 26 10:36:40 2006 Subject: [Histonet] whole villi Message-ID: Renee Do you need to embed and cut them as a circle? If not I would suggest that you slit along the length and then place the connective tissue (or muscle) side down on a piece of card. The pieces should stick to the card. Then fix, remove from card and process as normal. Once they are fixed they should remain flat. If you have really big pieces can pin these to a piece of cork and float tissue side down in the fixative - pin using stainless steel pins, plastic pins or hedgehog quills. You will always have trouble in getting sections with entire lengths of villi in the jejunum because of the long length of these villi in this region. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Till, Renee Sent: Friday, May 26, 2006 10:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] whole villi I sometimes work with rat and mouse gi tissues, and as you might expect one of the most time consuming things is getting the tissues embedded good. The colon has not been as much of a problem, but lately we are doing more with the jejunum and getting whole villi is enough to make me scream. It seems to me that one of my main problems is that sometime between the collection of the tissues (which is not done by me) and when I embed them, the tissues often curl up and it is hard to get a good straight piece to embed. We embed them on end? (so when cut it makes a little O). Any ideas on how to keep them straight during the processing? Or maybe if there is something else that I am not thinking of that could be a problem. Thanks. Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72002 Office (501)364-2785 Fax (501)364-3161 ======================================================================== ======================== Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ======================================================================== ======================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Fri May 26 10:43:42 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri May 26 10:43:57 2006 Subject: [Histonet] Re: Autofluorescence/Imunofluorescence protocols rat kidney In-Reply-To: <4476B843.20104@helsinki.fi> References: <4469b9af.7c.7cd1.1813511228@student.ucc.ie> <4476B843.20104@helsinki.fi> Message-ID: <6.0.0.22.1.20060526094057.01b56b50@gemini.msu.montana.edu> I have a question: what do you mean by "not effecting the signals from Alexa 488 and Alexa 546 too much?" Do you actually see some reduction in these two fluorophores? What about the fluoresceinated based fluorophores, FITC, Rhodamines, Texas Red? At 02:11 AM 5/26/2006, you wrote: >Hello! > >I recently evaluated several easy methods to quench autofluoresence for >paraformaldehyde-fixed paraffin sections of bovine tissues. >The only thing that worked (but worked beautifully!) was Sudan Black B (CI >26150). A 5-30 min incubation in a 0.1% solution in 70% ethanol, after the >whole immunofluorescence staining procedure, eliminated practically all >autofluorescence in any tissue, without affecting signals from alexa488 or >alexa546 too much. It's like magic! The only drawback was that it seems to >quench the DAPI counterstain. > >Just prepare the solution carefully, it won't solubilize very quickly. And >wash the slides with plenty of running water after the Sudan treatment. > >With best regards, >Mikael > >PS In addition to several chemical treatments, I also tried the recently >published method of irradiating the sections with fluorescent tubes of >specific colors. Did not have any effect at all. Anyone know what I could >have been doing wrong? > >-- >//////////////////////////////////////////////////////////// > > Mikael Niku URL: www.helsinki.fi/~mniku/ > University of Helsinki Dept. Basic Veterinary Sciences > > - Mit?k? mielt? olen l?nsimaisesta sivistyksest?? > Minusta se olisi erinomainen > ajatus! > - Gandhi > >//////////////////////////////////////////////////////////// > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From gentras <@t> vetmed.auburn.edu Fri May 26 11:28:23 2006 From: gentras <@t> vetmed.auburn.edu (Atoska S. Gentry) Date: Fri May 26 11:28:29 2006 Subject: [Histonet] free floating sections Message-ID: <7.0.1.0.0.20060526111755.019828d8@vetmed.auburn.edu> Hello, will someone please share with me your procedure for collecting & storing free floating frozen sections? This will be a first for me. Info gathered from histonet archives indicates the sections can be placed into cell culture plates with wells containing distilled water. Is distilled water required or will filtered deionized water be sufficient or better yet is it o.k. to place phosphate buffer into the wells? Also, please what's the maximum amount of time these free floating sections can be held at 4C in the culture plates before staining or whatever? I will be sectioning these sections for a researcher from main campus and I need to inform him. Thanks so much. Atoska From AliNeumann <@t> aol.com Fri May 26 11:46:55 2006 From: AliNeumann <@t> aol.com (AliNeumann@aol.com) Date: Fri May 26 11:47:11 2006 Subject: [Histonet] Histotech Position Denver Message-ID: <48b.f6394a.31a88aff@aol.com> AP laboratory in Arvada, CO is seeking a part-time (possibly will turn into full-time) experienced histotech to work 4am until finished on Tues-Sats. Please fax resume to 303-432-7866. Thank you. From mcauliff <@t> umdnj.edu Fri May 26 11:52:00 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri May 26 11:51:35 2006 Subject: [Histonet] whole villi In-Reply-To: <11F927674DEBDC43B960809A7403C5D2010CEDC7@MAILPED.ad.uams.edu> References: <11F927674DEBDC43B960809A7403C5D2010CEDC7@MAILPED.ad.uams.edu> Message-ID: <44773230.3040209@umdnj.edu> The tissue curls due to the smooth muscle in the wall of the gut. Pin the tissue out flat on a piece of cork sheet (available at Home Depot or Lowe's) then float, tissue side down, in fixative. Geoff Till, Renee wrote: >I sometimes work with rat and mouse gi tissues, and as you might expect >one of the most time consuming things is getting the tissues embedded >good. The colon has not been as much of a problem, but lately we are >doing more with the jejunum and getting whole villi is enough to make me >scream. It seems to me that one of my main problems is that sometime >between the collection of the tissues (which is not done by me) and when >I embed them, the tissues often curl up and it is hard to get a good >straight piece to embed. We embed them on end? (so when cut it makes a >little O). Any ideas on how to keep them straight during the processing? >Or maybe if there is something else that I am not thinking of that could >be a problem. Thanks. > > > >Renee' Till, HT > >Research Assistant > >Arkansas Children's Nutrition Center > >1212 Marshall St./N2021 > >Little Rock, AR 72002 > >Office (501)364-2785 > >Fax (501)364-3161 > > > > >================================================================================================ > >Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. >================================================================================================ >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From cwscouten <@t> myneurolab.com Fri May 26 11:54:23 2006 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Fri May 26 11:54:43 2006 Subject: [Histonet] free floating sections Message-ID: <5784D843593D874C93E9BADCB87342AB01306FFD@tpiserver03.Coretech-holdings.com> The following link is a product very useful for mananging and staining free floating sections. http://www.myneurolab.com/myneurolab/mnl_products_detail.asp?idproduct=4 64101&catdesc=Histology+Equipment&CatThreeID=635&CatOneID=4&subcatdesc=T issue+Staining+Systems&idsubcategory=192 Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Atoska S. Gentry Sent: Friday, May 26, 2006 11:28 AM To: Histonet Subject: [Histonet] free floating sections Hello, will someone please share with me your procedure for collecting & storing free floating frozen sections? This will be a first for me. Info gathered from histonet archives indicates the sections can be placed into cell culture plates with wells containing distilled water. Is distilled water required or will filtered deionized water be sufficient or better yet is it o.k. to place phosphate buffer into the wells? Also, please what's the maximum amount of time these free floating sections can be held at 4C in the culture plates before staining or whatever? I will be sectioning these sections for a researcher from main campus and I need to inform him. Thanks so much. Atoska _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sae2001 <@t> med.cornell.edu Fri May 26 11:58:31 2006 From: sae2001 <@t> med.cornell.edu (Scott A. Ely) Date: Fri May 26 11:58:42 2006 Subject: [Histonet] Ventant Benchmark Message-ID: <617169ca270f4.4476fb77@med.cornell.edu> We are considering buying some new equipment. I would like to know who uses the Ventana Benchmark immunostainer or other immunostainers and what you like and don't like about it. Scott Ely, MD MPH Section of Hematopathology Department of Pathology Weill Medical College of Cornell University New York Presbyterian Hospital 525 E. 68th Street New York, NY 10021 PH: 212-746-2442 Legal Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the original intended recipient(s) selected by Dr. Ely and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the recipient specified by Dr. Ely, please contact the sender by reply e-mail and destroy all copies of the original message. ----- Original Message ----- From: histonet-request@lists.utsouthwestern.edu Date: Friday, May 26, 2006 12:29 pm Subject: Histonet Digest, Vol 30, Issue 39 From sae2001 <@t> med.cornell.edu Fri May 26 12:01:54 2006 From: sae2001 <@t> med.cornell.edu (Scott A. Ely) Date: Fri May 26 12:02:00 2006 Subject: [Histonet] H&E per slide cost Message-ID: In looking at new H&E stainers / cover slippers, I have encountered some new machines that do not allow the use of 3rd party reagents or glass. The problem that presents is a high per slide cost for H&Es. I would like to know from group members: what is your total per slide cost for producing H&Es? Thanks. Scott Ely, MD MPH Section of Hematopathology Department of Pathology Weill Medical College of Cornell University New York Presbyterian Hospital 525 E. 68th Street New York, NY 10021 PH: 212-746-2442 Legal Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the original intended recipient(s) selected by Dr. Ely and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the recipient specified by Dr. Ely, please contact the sender by reply e-mail and destroy all copies of the original message. From JWEEMS <@t> sjha.org Fri May 26 12:08:32 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri May 26 12:08:08 2006 Subject: [Histonet] Red gram stain Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202736C3B@sjhaexc02.sjha.org> Hello All, One of our pathologists has just seen a reference to a "Red Gram Stain" for histoplasmosis in the Color Atlas of Surgical Pathology by Gutherie and Fawkes, pg 98, frame 298. Is anyone familiar with this stain? Thanks, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From TJJ <@t> Stowers-Institute.org Fri May 26 12:15:02 2006 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Fri May 26 12:15:27 2006 Subject: [Histonet] Re: Methyl green counterstain Message-ID: Jacqui, Methyl green does not like water, especially after the slides have been treated by HIER and immunostained. Side-by-side slides with & without pretreatment show good methyl green counterstaining without, and poor with. You can try using acetone to rinse and dehydrate your slides after staining. We find that works well, but it's very messy. Alternately, you can try using a 10% solution of hematoxylin for 30 seconds to counterstain, and it should keep the nuclei from getting too dark. If it's still too dark, adjust your time accordingly. If you're using AEC, be sure you don't use an alcoholic hematoxylin, or you run the risk of losing your positive signal. Best wishes, Teri Johnson Stowers Institute for Medical Research Kansas City, MO 64110 From la.sebree <@t> hosp.wisc.edu Fri May 26 12:20:14 2006 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Fri May 26 12:20:20 2006 Subject: [Histonet] Ventant Benchmark Message-ID: Scott, We have a Ventana NexES, BenchMark and BenchMark XT and couldn't be happier with them. They offer true walk-away technology, consistent, reproducible quality (including gorgeous ISH slides) and excellent customer service. About the only thing more we could ask of them is if they could offer continuous access, but then we'd probably never get a break! We are truly sold on this technology. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott A. Ely Sent: Friday, May 26, 2006 11:59 AM To: histonet@lists.utsouthwestern.edu Cc: histonet-owner@lists.utsouthwestern.edu Subject: [Histonet] Ventant Benchmark We are considering buying some new equipment. I would like to know who uses the Ventana Benchmark immunostainer or other immunostainers and what you like and don't like about it. Scott Ely, MD MPH Section of Hematopathology Department of Pathology Weill Medical College of Cornell University New York Presbyterian Hospital 525 E. 68th Street New York, NY 10021 PH: 212-746-2442 Legal Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the original intended recipient(s) selected by Dr. Ely and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the recipient specified by Dr. Ely, please contact the sender by reply e-mail and destroy all copies of the original message. ----- Original Message ----- From: histonet-request@lists.utsouthwestern.edu Date: Friday, May 26, 2006 12:29 pm Subject: Histonet Digest, Vol 30, Issue 39 From jbaez <@t> interscopepath.com Fri May 26 12:57:09 2006 From: jbaez <@t> interscopepath.com (Baez, Janet) Date: Fri May 26 12:57:33 2006 Subject: [Histonet] H&E per slide cost Message-ID: <9E956D8FEB06C2408B08AC16498325E9013A6F@adsl-67-113-77-28.dsl.lsan03.pacbell.net> We participated in the Laboratory Management Index Program (LMIP) provided by CAP in 2000. Our cost at that time was $7.00 per slide. Janet Baez Histology Manager Interscope Pathology Canoga Park, Ca. -----Original Message----- From: Scott A. Ely [mailto:sae2001@med.cornell.edu] Sent: Friday, May 26, 2006 10:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] H&E per slide cost In looking at new H&E stainers / cover slippers, I have encountered some new machines that do not allow the use of 3rd party reagents or glass. The problem that presents is a high per slide cost for H&Es. I would like to know from group members: what is your total per slide cost for producing H&Es? Thanks. Scott Ely, MD MPH Section of Hematopathology Department of Pathology Weill Medical College of Cornell University New York Presbyterian Hospital 525 E. 68th Street New York, NY 10021 PH: 212-746-2442 Legal Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the original intended recipient(s) selected by Dr. Ely and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the recipient specified by Dr. Ely, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kewleys <@t> health.missouri.edu Fri May 26 13:43:24 2006 From: kewleys <@t> health.missouri.edu (Kewley, Sharyl F.) Date: Fri May 26 13:43:29 2006 Subject: [Histonet] RE: Histonet Digest, Vol 30, Issue 38 RE: Hematoxylins ?? References: Message-ID: <99C56FE8929C8A47BDB4DAA43A1F1F79AE6FF5@UM-EMAIL05.um.umsystem.edu> Hi Steve, I have always ordered Sigma Aldrich "Certified Hematoxylin" 25 gm bottle and I have always been pleased with the results. Hope this is of some help. Sharyl Kewley, HT (ASCP) Columbia Regional Hospital 404 Keene St. Columbia, MO 65201 ________________________________ -------------------------- Message: 13 Date: Thu, 25 May 2006 09:12:22 -0700 (PDT) From: Steven Coakley Subject: [Histonet] hematoxylins ?? To: Histonet@lists.utsouthwestern.edu Message-ID: <20060525161222.28813.qmail@web38206.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Whats the best hematoxlyin powder/crystal to use in histology. I purchased a powder CI 175290 which contains OG powder ???. In making up weigerts Iron Hematoxylin I ended up using twice the recommended just to get the solution to stain properly. I will be making up a lead hematoxylin for pancreatic D cells and am starting to think I need a different hematoxylin. Steve --------------------------------- From ynwang <@t> u.washington.edu Fri May 26 14:03:02 2006 From: ynwang <@t> u.washington.edu (Yak-Nam Wang) Date: Fri May 26 14:02:05 2006 Subject: [Histonet] Nerve staining Message-ID: Hello all, I have 10% NBF fixed porcine tissue embedded in paraffin and want to stain for nerve fibers (specifically the celiac plexus). I have looked back at the archives and understand using the silver stains like Bodian's can be rather tricky so wanted to get peoples opinion on nerve staining. My questions are: 1) Is there another 'less tricky' stain that can be used for nerve fibers (methylene blue) ? Or a more user friendly method (Someone mentioned a Holmes technique)? 2) Any tips for getting over the tricky steps? 3) Has anyone tried the Bodian's 'kit' sold by Electron Microscopy Science? Thanks in advance for the insight. Yak-Nam Wang -- Research Associate CIMU Applied Physics Laboratory University of Washington Box 355640 Seattle WA 98195 Tel.: 206-616-6673 From rjbuesa <@t> yahoo.com Fri May 26 14:29:54 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri May 26 14:29:58 2006 Subject: [Histonet] H&E per slide cost In-Reply-To: Message-ID: <20060526192954.62632.qmail@web61223.mail.yahoo.com> Scott: I am attached on separate cover a report on histology general costs that I hope will be useful for you. Ren? J. "Scott A. Ely" wrote: In looking at new H&E stainers / cover slippers, I have encountered some new machines that do not allow the use of 3rd party reagents or glass. The problem that presents is a high per slide cost for H&Es. I would like to know from group members: what is your total per slide cost for producing H&Es? Thanks. Scott Ely, MD MPH Section of Hematopathology Department of Pathology Weill Medical College of Cornell University New York Presbyterian Hospital 525 E. 68th Street New York, NY 10021 PH: 212-746-2442 Legal Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the original intended recipient(s) selected by Dr. Ely and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the recipient specified by Dr. Ely, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1¢/min. From rjbuesa <@t> yahoo.com Fri May 26 14:42:13 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri May 26 14:42:17 2006 Subject: [Histonet] Ventant Benchmark In-Reply-To: <617169ca270f4.4476fb77@med.cornell.edu> Message-ID: <20060526194213.398.qmail@web61222.mail.yahoo.com> Scott: I have worked with the Ventana Benchmark and the Dako autostainer. The Benchmark is a "hands-off" or "tech-away" technology but I prefer the Dako autostainer. Ren? J. "Scott A. Ely" wrote: We are considering buying some new equipment. I would like to know who uses the Ventana Benchmark immunostainer or other immunostainers and what you like and don't like about it. Scott Ely, MD MPH Section of Hematopathology Department of Pathology Weill Medical College of Cornell University New York Presbyterian Hospital 525 E. 68th Street New York, NY 10021 PH: 212-746-2442 Legal Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the original intended recipient(s) selected by Dr. Ely and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the recipient specified by Dr. Ely, please contact the sender by reply e-mail and destroy all copies of the original message. ----- Original Message ----- From: histonet-request@lists.utsouthwestern.edu Date: Friday, May 26, 2006 12:29 pm Subject: Histonet Digest, Vol 30, Issue 39 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ring'em or ping'em. Make PC-to-phone calls as low as 1?/min with Yahoo! Messenger with Voice. From rjbuesa <@t> yahoo.com Fri May 26 14:51:15 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri May 26 14:51:20 2006 Subject: [Histonet] Nerve staining In-Reply-To: Message-ID: <20060526195115.86201.qmail@web61214.mail.yahoo.com> Under separate cover I am sending you a Bodian procedure that can be completed in less than 2 hours and never fails. Ren? J. Yak-Nam Wang wrote: Hello all, I have 10% NBF fixed porcine tissue embedded in paraffin and want to stain for nerve fibers (specifically the celiac plexus). I have looked back at the archives and understand using the silver stains like Bodian's can be rather tricky so wanted to get peoples opinion on nerve staining. My questions are: 1) Is there another 'less tricky' stain that can be used for nerve fibers (methylene blue) ? Or a more user friendly method (Someone mentioned a Holmes technique)? 2) Any tips for getting over the tricky steps? 3) Has anyone tried the Bodian's 'kit' sold by Electron Microscopy Science? Thanks in advance for the insight. Yak-Nam Wang -- Research Associate CIMU Applied Physics Laboratory University of Washington Box 355640 Seattle WA 98195 Tel.: 206-616-6673 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Feel free to call! Free PC-to-PC calls. Low rates on PC-to-Phone. Get Yahoo! Messenger with Voice From carl.hobbs <@t> kcl.ac.uk Fri May 26 14:58:47 2006 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Fri May 26 14:59:02 2006 Subject: [Histonet] Re:looking for oligodendrocyte antibody for FS Message-ID: I haven't tried this on FS but it appears to be selective for a narrow range of CNS cells, on pwax sections, after HIER. Take a look at Calbiochem's spec sheet for APC ( # OP80). Also , I posted a couple of pics here http://www.immunoportal.com/index.php using their Ab on pwax sections.It is not exclusive for oligodendrocytes but , despite the spec sheet stating that it will, I haven't noted any astrocyte labelling so far. Hit "picture gallery" and type in "APC" in the search box. I would be interested in your opinion and anyone else's, regarding the oligo "fidelity" of the staining.( not my area) I have no experience of the above Ab reagent's reactivity after using Zamboni's fluid fixation. The incorporation of Picric acid to fixing fluids seems to be accepted as an "accentuator" of immunoreactivity, in many cases. In my humble opinion and limited experience, I find it's incorporation to be detrimental to achieving optimal immuno reactivity in pwax and also frozen sections. Best wishes carl From mbmphoto <@t> gmail.com Fri May 26 20:50:53 2006 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Fri May 26 20:50:14 2006 Subject: [Histonet] free floating sections In-Reply-To: <7.0.1.0.0.20060526111755.019828d8@vetmed.auburn.edu> References: <7.0.1.0.0.20060526111755.019828d8@vetmed.auburn.edu> Message-ID: <193CA49C-2E3F-45F3-AFE8-5A7B15EED0BE@gmail.com> Hello Atoska, I section serial sections of monkey & rat brain on the cryostat @ 40um. I collect the sections using 24 plate wells containing a solution called "anti-freeze." Each well has about 2300ul per well (don't over- fill) This working anti-freeze solution contains: ethylene glycol - 120ml gycerol - 120 working PBS (I can't remember the correct amount - will email this information when I get back to the lab on Tuesday). When mixing this solution - mix for at least 30 minutes and store in refrig @ 4C. Solution is stable. Anyway, when I use the cryostat to cut brain tissue, I keep a small glass beaker (50ml size) inside the cryostat with about 20-30ml of anti-freezer solution. Before I start sectioning, I dip my sable brush into this solution and brush the knife surface very close to the edge w/some anti-freeze solution, not a lot, but just enough so that when I do take a section it slides on the knife. I then use my brush moist with anti-freeze to pick-up the section and place into a plate well until each well has a section. Repeat until an X number of plate well are used for each case. We use 5 to 6 plate wells (one plate has 24 wells) per monkey case. When the case has been completely section, each plate well (each has ID info) is then sealed with cryo-tape. The 5 or 6 plates are then stacked on top of each other and wrapped in aluminum foil with ID info., and sealed with more cryo-tape. The plates are then placed inside a large enough plastic box w/lid & ID to hold more than one case. Box with plate wells are kept in a refrig @ 4C. They will keep for several years and if sealed properly this solution will not evaporated. When ever we need to do some histochemical and/or IHC staining, we simply remove the sections of interests and wash the sections in 3 changes of working PBS - 5 minutes each and proceed with the staining. I hope this helps and on Tuesday I will email you the complete recipe for making anti-freeze solution. P.S. When I finish using the cryostat, I simply wipe any residual anti-freeze from the knife holder & knife using kim-wipes with 70% alcohol and then 100% alcohol. Yours Maria Bartola Mejia University of California San Francisco Department of Neurosurgery San Francisco, CA 94103 On May 26, 2006, at 9:28 AM, Atoska S. Gentry wrote: > Hello, will someone please share with me your procedure for > collecting & storing free floating frozen sections? This will be a > first for me. Info gathered from histonet archives indicates the > sections can be placed into cell culture plates with wells > containing distilled water. Is distilled water required or will > filtered deionized water be sufficient or better yet is it o.k. to > place phosphate buffer into the wells? Also, please what's the > maximum amount of time these free floating sections can be held at > 4C in the culture plates before staining or whatever? I will be > sectioning these sections for a researcher from main campus and I > need to inform him. Thanks so much. Atoska > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Sat May 27 08:29:28 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sat May 27 08:29:33 2006 Subject: [Histonet] Ventant Benchmark References: <617169ca270f4.4476fb77@med.cornell.edu> Message-ID: <006701c68191$949c4c70$1bb30b43@yourxhtr8hvc4p> flaming time How come every one talks about the reliability and walk away technology, but no one ever talks about the price of running these machines? Shouldn't cost be a factor also? I've been in budget meetings all week. The only thing I heard was how expensive it is to run immunos. Yeah, we have two XTs, what do you expect? The opinions stated here are that of the author only and does not express the opinions of his employer, lawyers, siblings, significant others and their lawyers. Oh, by the way, I told the same thing to my Ventana Rep on Friday. He knows, so y'all don't need to call my CEO. Y'all know who you are. Let the flaming begin. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Scott A. Ely" To: Cc: Sent: Friday, May 26, 2006 11:58 AM Subject: [Histonet] Ventant Benchmark > We are considering buying some new equipment. I would like to know who > uses the Ventana Benchmark > immunostainer or other immunostainers and what you like and don't like > about it. > > Scott Ely, MD MPH > Section of Hematopathology > Department of Pathology > Weill Medical College of Cornell University > New York Presbyterian Hospital > 525 E. 68th Street > New York, NY 10021 > PH: 212-746-2442 > > Legal Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the original > intended recipient(s) selected by Dr. Ely and may contain confidential and > privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If you > are not the recipient specified by Dr. > Ely, please contact the sender by reply e-mail and destroy all copies of > the original message. > > ----- Original Message ----- > From: histonet-request@lists.utsouthwestern.edu > Date: Friday, May 26, 2006 12:29 pm > Subject: Histonet Digest, Vol 30, Issue 39 > > -------------------------------------------------------------------------------- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -------------------------------------------------------------------------------- > -------------------------------------------------------------------------------- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.7.1/348 - Release Date: 5/25/2006 From pruegg <@t> ihctech.net Sat May 27 10:25:53 2006 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Sat May 27 10:26:07 2006 Subject: [Histonet] Ventant Benchmark In-Reply-To: <006701c68191$949c4c70$1bb30b43@yourxhtr8hvc4p> Message-ID: <200605271526.k4RFPv5L096277@pro12.abac.com> I am with you Joe. I have not ever been able to understand how the labs can justify spending so much to run IHC, I guess they just pass the cost on to the patient, I can do the same thing for about 1/100 of the cost of running an instrument like the Ventana using an open system like the Dakoautostainer and making up a lot of my own reagents like the buffers. I work in research and don't have a money cow like the patient health care system to milk. No wonder health care costs are so,years ago when I worked in clinical we used to do what ever we could to try and help save the patient expense, sure doesn't seem like that is the case anymore. I know that understaffing, quick turn around and untrained personnel is a big part of the problem here but come on, do you really want someone who only knows how to apply a bar code to be running your diagnostic IHC's? Bring on the flaming...... Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Saturday, May 27, 2006 7:29 AM To: Scott A. Ely; histonet@lists.utsouthwestern.edu Cc: histonet-owner@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventant Benchmark flaming time How come every one talks about the reliability and walk away technology, but no one ever talks about the price of running these machines? Shouldn't cost be a factor also? I've been in budget meetings all week. The only thing I heard was how expensive it is to run immunos. Yeah, we have two XTs, what do you expect? The opinions stated here are that of the author only and does not express the opinions of his employer, lawyers, siblings, significant others and their lawyers. Oh, by the way, I told the same thing to my Ventana Rep on Friday. He knows, so y'all don't need to call my CEO. Y'all know who you are. Let the flaming begin. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Scott A. Ely" To: Cc: Sent: Friday, May 26, 2006 11:58 AM Subject: [Histonet] Ventant Benchmark > We are considering buying some new equipment. I would like to know who > uses the Ventana Benchmark > immunostainer or other immunostainers and what you like and don't like > about it. > > Scott Ely, MD MPH > Section of Hematopathology > Department of Pathology > Weill Medical College of Cornell University > New York Presbyterian Hospital > 525 E. 68th Street > New York, NY 10021 > PH: 212-746-2442 > > Legal Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the original > intended recipient(s) selected by Dr. Ely and may contain confidential and > privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If you > are not the recipient specified by Dr. > Ely, please contact the sender by reply e-mail and destroy all copies of > the original message. > > ----- Original Message ----- > From: histonet-request@lists.utsouthwestern.edu > Date: Friday, May 26, 2006 12:29 pm > Subject: Histonet Digest, Vol 30, Issue 39 > > ---------------------------------------------------------------------------- ---- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ---------------------------------------------------------------------------- ---- > ---------------------------------------------------------------------------- ---- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.7.1/348 - Release Date: 5/25/2006 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Malcolm.McCallum <@t> tamut.edu Sat May 27 10:39:09 2006 From: Malcolm.McCallum <@t> tamut.edu (Malcolm McCallum) Date: Sat May 27 10:40:51 2006 Subject: [Histonet] Ventant Benchmark References: <617169ca270f4.4476fb77@med.cornell.edu> <006701c68191$949c4c70$1bb30b43@yourxhtr8hvc4p> Message-ID: Undoubtedly the cost and maintenance of automation is cheaper than the cost of additional employees salaries and benefits accompanied by associated duplication of manual systems. Additionally, equipment is depreciable, whereas human resources are a cost. VISIT THE JOURNAL HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-223-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Joe Nocito Sent: Sat 5/27/2006 8:29 AM To: Scott A. Ely; histonet@lists.utsouthwestern.edu Cc: histonet-owner@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventant Benchmark flaming time How come every one talks about the reliability and walk away technology, but no one ever talks about the price of running these machines? Shouldn't cost be a factor also? I've been in budget meetings all week. The only thing I heard was how expensive it is to run immunos. Yeah, we have two XTs, what do you expect? The opinions stated here are that of the author only and does not express the opinions of his employer, lawyers, siblings, significant others and their lawyers. Oh, by the way, I told the same thing to my Ventana Rep on Friday. He knows, so y'all don't need to call my CEO. Y'all know who you are. Let the flaming begin. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Scott A. Ely" To: Cc: Sent: Friday, May 26, 2006 11:58 AM Subject: [Histonet] Ventant Benchmark > We are considering buying some new equipment. I would like to know who > uses the Ventana Benchmark > immunostainer or other immunostainers and what you like and don't like > about it. > > Scott Ely, MD MPH > Section of Hematopathology > Department of Pathology > Weill Medical College of Cornell University > New York Presbyterian Hospital > 525 E. 68th Street > New York, NY 10021 > PH: 212-746-2442 > > Legal Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the original > intended recipient(s) selected by Dr. Ely and may contain confidential and > privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If you > are not the recipient specified by Dr. > Ely, please contact the sender by reply e-mail and destroy all copies of > the original message. > > ----- Original Message ----- > From: histonet-request@lists.utsouthwestern.edu > Date: Friday, May 26, 2006 12:29 pm > Subject: Histonet Digest, Vol 30, Issue 39 > > -------------------------------------------------------------------------------- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -------------------------------------------------------------------------------- > -------------------------------------------------------------------------------- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.7.1/348 - Release Date: 5/25/2006 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mstahl <@t> bvha.org Sat May 27 11:26:31 2006 From: mstahl <@t> bvha.org (Stahl, Michael) Date: Sat May 27 11:26:23 2006 Subject: [Histonet] Ventant Benchmark Message-ID: <4C878E714B21EB4F8EB159777B8822EE287E99@bvfyms01.net.bvha.org> Ill have to put my kudos in for the Ventana machines also. We currently have the Benchmark Immuno Stainer and the NeXus, and they are the workhorses of our lab. Not only are the slides always consistent in staining, but with the internal programs in the software QC is much easier. Don't get me wrong, I'm not saying manual staining is not a viable option, nor am I saying that automatic stainers can do it better. The difference is, in "walkaways, while the slides are staining I can do my frozen section, inventory, ordering of supplies, registration of out patients, logging in of surgical specimens, and keep my pathologist happy by being on hand to provide for them what they require from a Histotech. One further note, all 4 of the pathologist here love the quality of both the Immuno and special stains. Michael P. Stahl HT(ASCP) BVRHC Findlay,OH 45840 From johnwhull <@t> gmail.com Sat May 27 12:06:48 2006 From: johnwhull <@t> gmail.com (John Hull) Date: Sat May 27 12:06:53 2006 Subject: [Histonet] H&E per slide cost In-Reply-To: <9E956D8FEB06C2408B08AC16498325E9013A6F@adsl-67-113-77-28.dsl.lsan03.pacbell.net> References: <9E956D8FEB06C2408B08AC16498325E9013A6F@adsl-67-113-77-28.dsl.lsan03.pacbell.net> Message-ID: <671b2cca0605271006j3a869fc2g399db85cecba35c5@mail.gmail.com> Would I be able to find that study, the Laboratory Management Index Program, on the CAP website? I am very interested to see their take on staining costs from H&E to immunos. On 5/26/06, Baez, Janet wrote: > > > We participated in the Laboratory Management Index Program (LMIP) > provided by CAP in 2000. Our cost at that time was $7.00 per slide. > > Janet Baez > Histology Manager > Interscope Pathology > Canoga Park, Ca. > > -----Original Message----- > From: Scott A. Ely [mailto:sae2001@med.cornell.edu] > Sent: Friday, May 26, 2006 10:02 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] H&E per slide cost > > In looking at new H&E stainers / cover slippers, I have encountered some > new machines that do not allow the use of > 3rd party reagents or glass. The problem that presents is a high per > slide cost for H&Es. I would like to know from > group members: what is your total per slide cost for producing H&Es? > > Thanks. > > Scott Ely, MD MPH > Section of Hematopathology > Department of Pathology > Weill Medical College of Cornell University > New York Presbyterian Hospital > 525 E. 68th Street > New York, NY 10021 > PH: 212-746-2442 > > Legal Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the original > intended recipient(s) selected by Dr. Ely and may contain confidential > and privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If > you are not the recipient specified by Dr. > Ely, please contact the sender by reply e-mail and destroy all copies of > the original message. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From jnocito <@t> satx.rr.com Sat May 27 17:56:31 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sat May 27 17:56:43 2006 Subject: [Histonet] Ventant Benchmark References: <200605271526.k4RFPv5L096277@pro12.abac.com> Message-ID: <001d01c681e0$cc3c0220$1bb30b43@yourxhtr8hvc4p> Patsy, we charge what Medicare pays. Many insurance companies pay less. This is what fries my cookies. These people want to make money, yet pays trough the nose for immunos. Penny wise, dollar foolish is what my father would say. Joe ----- Original Message ----- From: To: "'Joe Nocito'" ; "'Scott A. Ely'" ; Cc: Sent: Saturday, May 27, 2006 10:25 AM Subject: RE: [Histonet] Ventant Benchmark >I am with you Joe. > I have not ever been able to understand how the labs can justify spending > so > much to run IHC, I guess they just pass the cost on to the patient, I can > do > the same thing for about 1/100 of the cost of running an instrument like > the > Ventana using an open system like the Dakoautostainer and making up a lot > of > my own reagents like the buffers. I work in research and don't have a > money > cow like the patient health care system to milk. No wonder health care > costs are so,years ago when I worked in clinical we used to do what ever > we > could to try and help save the patient expense, sure doesn't seem like > that > is the case anymore. I know that understaffing, quick turn around and > untrained personnel is a big part of the problem here but come on, do you > really want someone who only knows how to apply a bar code to be running > your diagnostic IHC's? > Bring on the flaming...... > Patsy > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito > Sent: Saturday, May 27, 2006 7:29 AM > To: Scott A. Ely; histonet@lists.utsouthwestern.edu > Cc: histonet-owner@lists.utsouthwestern.edu > Subject: Re: [Histonet] Ventant Benchmark > > flaming time > How come every one talks about the reliability and walk away technology, > but > > no one ever talks about the price of running these machines? Shouldn't > cost > be a factor also? > I've been in budget meetings all week. The only thing I heard was how > expensive it is to run immunos. Yeah, we have two XTs, what do you expect? > > The opinions stated here are that of the author only and does not express > the opinions of his employer, lawyers, siblings, significant others and > their lawyers. > > Oh, by the way, I told the same thing to my Ventana Rep on Friday. He > knows, > > so y'all don't need to call my CEO. Y'all know who you are. > > Let the flaming begin. > > Joe Nocito BS, HT(ASCP)QIHC > Histology Manager > Pathology Reference Lab > San Antonio, TX > ----- Original Message ----- > From: "Scott A. Ely" > To: > Cc: > Sent: Friday, May 26, 2006 11:58 AM > Subject: [Histonet] Ventant Benchmark > > >> We are considering buying some new equipment. I would like to know who >> uses the Ventana Benchmark >> immunostainer or other immunostainers and what you like and don't like >> about it. >> >> Scott Ely, MD MPH >> Section of Hematopathology >> Department of Pathology >> Weill Medical College of Cornell University >> New York Presbyterian Hospital >> 525 E. 68th Street >> New York, NY 10021 >> PH: 212-746-2442 >> >> Legal Confidentiality Notice: This e-mail message, including any >> attachments, is for the sole use of the original >> intended recipient(s) selected by Dr. Ely and may contain confidential >> and > >> privileged information. Any >> unauthorized review, use, disclosure or distribution is prohibited. If >> you > >> are not the recipient specified by Dr. >> Ely, please contact the sender by reply e-mail and destroy all copies of >> the original message. >> >> ----- Original Message ----- >> From: histonet-request@lists.utsouthwestern.edu >> Date: Friday, May 26, 2006 12:29 pm >> Subject: Histonet Digest, Vol 30, Issue 39 >> >> > > > ---------------------------------------------------------------------------- > ---- > > >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > ---------------------------------------------------------------------------- > ---- > > >> > > > ---------------------------------------------------------------------------- > ---- > > > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.394 / Virus Database: 268.7.1/348 - Release Date: 5/25/2006 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.394 / Virus Database: 268.7.2/349 - Release Date: 5/26/2006 > > From jnocito <@t> satx.rr.com Sat May 27 17:58:50 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sat May 27 17:58:58 2006 Subject: [Histonet] Ventant Benchmark References: <617169ca270f4.4476fb77@med.cornell.edu> <006701c68191$949c4c70$1bb30b43@yourxhtr8hvc4p> Message-ID: <002301c681e1$1ee86810$1bb30b43@yourxhtr8hvc4p> I'm not saying do it manually. I'm saying that there are less expensive ways to perform immunos than using Ventana's high cost reagents. Joe ----- Original Message ----- From: "Malcolm McCallum" To: "Joe Nocito" ; "Scott A. Ely" ; Cc: Sent: Saturday, May 27, 2006 10:39 AM Subject: RE: [Histonet] Ventant Benchmark Undoubtedly the cost and maintenance of automation is cheaper than the cost of additional employees salaries and benefits accompanied by associated duplication of manual systems. Additionally, equipment is depreciable, whereas human resources are a cost. VISIT THE JOURNAL HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-223-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Joe Nocito Sent: Sat 5/27/2006 8:29 AM To: Scott A. Ely; histonet@lists.utsouthwestern.edu Cc: histonet-owner@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventant Benchmark flaming time How come every one talks about the reliability and walk away technology, but no one ever talks about the price of running these machines? Shouldn't cost be a factor also? I've been in budget meetings all week. The only thing I heard was how expensive it is to run immunos. Yeah, we have two XTs, what do you expect? The opinions stated here are that of the author only and does not express the opinions of his employer, lawyers, siblings, significant others and their lawyers. Oh, by the way, I told the same thing to my Ventana Rep on Friday. He knows, so y'all don't need to call my CEO. Y'all know who you are. Let the flaming begin. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Scott A. Ely" To: Cc: Sent: Friday, May 26, 2006 11:58 AM Subject: [Histonet] Ventant Benchmark > We are considering buying some new equipment. I would like to know who > uses the Ventana Benchmark > immunostainer or other immunostainers and what you like and don't like > about it. > > Scott Ely, MD MPH > Section of Hematopathology > Department of Pathology > Weill Medical College of Cornell University > New York Presbyterian Hospital > 525 E. 68th Street > New York, NY 10021 > PH: 212-746-2442 > > Legal Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the original > intended recipient(s) selected by Dr. Ely and may contain confidential and > privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If you > are not the recipient specified by Dr. > Ely, please contact the sender by reply e-mail and destroy all copies of > the original message. > > ----- Original Message ----- > From: histonet-request@lists.utsouthwestern.edu > Date: Friday, May 26, 2006 12:29 pm > Subject: Histonet Digest, Vol 30, Issue 39 > > -------------------------------------------------------------------------------- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -------------------------------------------------------------------------------- > -------------------------------------------------------------------------------- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.7.1/348 - Release Date: 5/25/2006 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.7.2/349 - Release Date: 5/26/2006 From gu.lang <@t> gmx.at Sun May 28 02:58:05 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sun May 28 02:58:06 2006 Subject: AW: [Histonet] Ventana Benchmark In-Reply-To: <002301c681e1$1ee86810$1bb30b43@yourxhtr8hvc4p> Message-ID: <000701c6822c$73e0ebd0$eeeea8c0@SERVER01> First you are fascinated by the wonderful quality, go-away-system, easy to handle technique, fast results. Doctors see constant quality, less staff needed and therefore decrease of costs (?). And then, they want a special antibody to work - hmm. And then, the bill comes in. And now the complains go on. The technician, who is soo expensive, has to look for ways to make the system cheaper. Each sticker on the slides is counted, and each drop of antibody is saved. And there hangs the damokles-sword above our heads, that the next job-position will be shortened. "Make it cheaper - or your job is lost". It would have been better to evaluate the follow-costs before complaining, yes? I don't like the dependence on a single company - but I like the good staining-quality. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Joe Nocito Gesendet: Sonntag, 28. Mai 2006 00:59 An: Malcolm McCallum; Scott A. Ely; histonet@lists.utsouthwestern.edu Cc: histonet-owner@lists.utsouthwestern.edu Betreff: Re: [Histonet] Ventant Benchmark I'm not saying do it manually. I'm saying that there are less expensive ways to perform immunos than using Ventana's high cost reagents. Joe ----- Original Message ----- From: "Malcolm McCallum" To: "Joe Nocito" ; "Scott A. Ely" ; Cc: Sent: Saturday, May 27, 2006 10:39 AM Subject: RE: [Histonet] Ventant Benchmark Undoubtedly the cost and maintenance of automation is cheaper than the cost of additional employees salaries and benefits accompanied by associated duplication of manual systems. Additionally, equipment is depreciable, whereas human resources are a cost. VISIT THE JOURNAL HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-223-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Joe Nocito Sent: Sat 5/27/2006 8:29 AM To: Scott A. Ely; histonet@lists.utsouthwestern.edu Cc: histonet-owner@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventant Benchmark flaming time How come every one talks about the reliability and walk away technology, but no one ever talks about the price of running these machines? Shouldn't cost be a factor also? I've been in budget meetings all week. The only thing I heard was how expensive it is to run immunos. Yeah, we have two XTs, what do you expect? The opinions stated here are that of the author only and does not express the opinions of his employer, lawyers, siblings, significant others and their lawyers. Oh, by the way, I told the same thing to my Ventana Rep on Friday. He knows, so y'all don't need to call my CEO. Y'all know who you are. Let the flaming begin. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Scott A. Ely" To: Cc: Sent: Friday, May 26, 2006 11:58 AM Subject: [Histonet] Ventant Benchmark > We are considering buying some new equipment. I would like to know who > uses the Ventana Benchmark > immunostainer or other immunostainers and what you like and don't like > about it. > > Scott Ely, MD MPH > Section of Hematopathology > Department of Pathology > Weill Medical College of Cornell University > New York Presbyterian Hospital > 525 E. 68th Street > New York, NY 10021 > PH: 212-746-2442 > > Legal Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the original > intended recipient(s) selected by Dr. Ely and may contain confidential and > privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If you > are not the recipient specified by Dr. > Ely, please contact the sender by reply e-mail and destroy all copies of > the original message. > > ----- Original Message ----- > From: histonet-request@lists.utsouthwestern.edu > Date: Friday, May 26, 2006 12:29 pm > Subject: Histonet Digest, Vol 30, Issue 39 > > ---------------------------------------------------------------------------- ---- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ---------------------------------------------------------------------------- ---- > ---------------------------------------------------------------------------- ---- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.7.1/348 - Release Date: 5/25/2006 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.7.2/349 - Release Date: 5/26/2006 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rkpulsifer <@t> yahoo.com Sun May 28 09:17:21 2006 From: rkpulsifer <@t> yahoo.com (Robyn Pulsifer) Date: Sun May 28 09:17:24 2006 Subject: [Histonet] Cirrhosis of the Liver Message-ID: <20060528141721.31950.qmail@web53109.mail.yahoo.com> Hi I am an Argosy University student and I'm doing a Histotechnology Capstone on Cirrhosis. I was wondering if anyone had some information that I could use on this disease that Farida didn't mention in her book, and with the experience that you histo's and pathologists have had. Anything would be great. Thank you!! Sincerely, Robyn --------------------------------- Sneak preview the all-new Yahoo.com. It's not radically different. Just radically better. From JWEEMS <@t> sjha.org Sun May 28 12:34:50 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Sun May 28 12:34:26 2006 Subject: [Histonet] Ventant Benchmark References: <617169ca270f4.4476fb77@med.cornell.edu><006701c68191$949c4c70$1bb30b43@yourxhtr8hvc4p> <002301c681e1$1ee86810$1bb30b43@yourxhtr8hvc4p> Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD320263E11C@sjhaexc02.sjha.org> That's what I was saying too. Even with all our cost cutting attempts, do enough immunos to support 3 reagent-leased DAKO instruments. They're walk away too = after you get them loaded anyway! - and the reagents are not nearly as expensive. We titer all the antibodies that are available - a savings over the prepared Abs. The company has been a great partner for our laboratory. j Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Joe Nocito Sent: Sat 5/27/2006 6:58 PM To: Malcolm McCallum; Scott A. Ely; histonet@lists.utsouthwestern.edu Cc: histonet-owner@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventant Benchmark I'm not saying do it manually. I'm saying that there are less expensive ways to perform immunos than using Ventana's high cost reagents. Joe ----- Original Message ----- From: "Malcolm McCallum" To: "Joe Nocito" ; "Scott A. Ely" ; Cc: Sent: Saturday, May 27, 2006 10:39 AM Subject: RE: [Histonet] Ventant Benchmark Undoubtedly the cost and maintenance of automation is cheaper than the cost of additional employees salaries and benefits accompanied by associated duplication of manual systems. Additionally, equipment is depreciable, whereas human resources are a cost. VISIT THE JOURNAL HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-223-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Joe Nocito Sent: Sat 5/27/2006 8:29 AM To: Scott A. Ely; histonet@lists.utsouthwestern.edu Cc: histonet-owner@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventant Benchmark flaming time How come every one talks about the reliability and walk away technology, but no one ever talks about the price of running these machines? Shouldn't cost be a factor also? I've been in budget meetings all week. The only thing I heard was how expensive it is to run immunos. Yeah, we have two XTs, what do you expect? The opinions stated here are that of the author only and does not express the opinions of his employer, lawyers, siblings, significant others and their lawyers. Oh, by the way, I told the same thing to my Ventana Rep on Friday. He knows, so y'all don't need to call my CEO. Y'all know who you are. Let the flaming begin. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Scott A. Ely" To: Cc: Sent: Friday, May 26, 2006 11:58 AM Subject: [Histonet] Ventant Benchmark > We are considering buying some new equipment. I would like to know who > uses the Ventana Benchmark > immunostainer or other immunostainers and what you like and don't like > about it. > > Scott Ely, MD MPH > Section of Hematopathology > Department of Pathology > Weill Medical College of Cornell University > New York Presbyterian Hospital > 525 E. 68th Street > New York, NY 10021 > PH: 212-746-2442 > > Legal Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the original > intended recipient(s) selected by Dr. Ely and may contain confidential and > privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If you > are not the recipient specified by Dr. > Ely, please contact the sender by reply e-mail and destroy all copies of > the original message. > > ----- Original Message ----- > From: histonet-request@lists.utsouthwestern.edu > Date: Friday, May 26, 2006 12:29 pm > Subject: Histonet Digest, Vol 30, Issue 39 > > -------------------------------------------------------------------------------- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -------------------------------------------------------------------------------- > -------------------------------------------------------------------------------- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.7.1/348 - Release Date: 5/25/2006 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.7.2/349 - Release Date: 5/26/2006 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From p_bourne_14526 <@t> yahoo.com Sun May 28 15:05:47 2006 From: p_bourne_14526 <@t> yahoo.com (Patricia Bourne) Date: Sun May 28 15:05:51 2006 Subject: [Histonet] Ventant Benchmark In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD320263E11C@sjhaexc02.sjha.org> Message-ID: <20060528200547.23755.qmail@web54214.mail.yahoo.com> I agree. We also have three Dako autostainers and they are the workhorse of our lab. They certainly keep the cost per slide down and we also have a great relationship with the company. We also, however, have a Ventana XT which does great in-situ. I do find however, the cost per test is expensive. I also find the machine very noise and complaints come in on a daily basis. I find the success with working with Ventana to be the sales rep. We are very pleased with Dako and are excited about their new instrument. We appreciate the open system. Since we perform a great deal of research, the cost per slide is extremely important to our researchers on a very limited budget. As with any instrument there are pro's and con's. __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From brian.chelack <@t> usask.ca Sun May 28 15:55:17 2006 From: brian.chelack <@t> usask.ca (Brian Chelack) Date: Sun May 28 15:57:49 2006 Subject: [Histonet] Ventana Benchmark closed systems vs Open system stainers - a diatribe Message-ID: <000101c68299$066b2490$4f13e980@PDS11> It is interesting to note how firmly people plant themselves on one side or the other of the "Ventana Issues". I have worked with many different immunostainers, going back to the old capillary gap Fisher Code-Ons. In my estimation every company that makes a stainer has to make some sort of compromise when it comes to its design. Every stainer has its own set of strengths and weaknesses; I have yet to see one that is "perfect". As technologists we all want to see the perfect machine that allows us to load individual slides whenever we want, process them completely, error free and automatically right down to the coverslip, and do it for next to nothing. As an added feature the stainer should allow all the IHC technologists to maintain the "aura" of being highly skilled irreplaceable technical artists who can perform magic on paraffin sections. This means job security because department supervisors are certain their labs require the technologists who are busily tending to their "automated" machines. This "perfect" company then must sell us one of these instruments and be prepared to walk away from the marketplace, because we technologists don't want to be "beholdin" to them for any further purchases. We are mercenaries who will abandon them in an instant for the next company that offers us reagents for 3% cost savings. So if I am this company what should I do? Why should I invest a great deal of time and money into developing a stainer that provides me with no chance for an ongoing revenue stream? I have seen companies with good technology (for their times), fade away due to an inability to maintain a healthy cash flow. Patents are sold, other companies try to adapt the technology to their product lines and life staggers on. On the other hand, companies that keep their reagents proprietary, and yes expensive, do have the money available to develop their product lines and look at new ideas. I think companies like Ventana, do charge a lot of money for their reagents, but I also see how companies like Dako, with their new Artisan stainer is slowly moving towards generating ongoing revenue streams. Watch for the convergence! Which one is the best choice? Well it depends. Every lab requires at least one person to turn on the lights and make coffee, so this person is also available to perform IHC staining. Typically an IHC lab begins with someone doing the IHC staining by hand. If this person is at least moderately skilled the lab becomes successful and requests for stains start arriving, and arriving and arriving. Another skilled technologist is hired to no avail, before long, both are swamped and begging for more help, but budgets are always tight so it must be time for an automated IHC stainer. What should we choose? Well the ability to stain large numbers of slides is important, right? So we look at models that offer the ability to stain many slides at once, but these models can only output their slides once or twice per day. This means you need to keep your two skilled technologists around in order to manage the large bulges of work expelled by the stainers periodically throughout the day. Is speed important? Well of course it is, but turn around times are inversely proportional to the capacity of the equipment. Think about what happens to the slide that arrives at the stainer 10 minutes after you hit the start button, for that that matter, think about what happens to the first slide that arrives at the stainer and then waits until enough other slides are present to warrant starting the equipment. It is becoming apparent that single piece flow is the most efficient method for improving turn around times, but to my knowledge, all the equipment makers (Ventana included, see the new BenchMark XT), believe that more slides in a batch is better. Histo techs cut one block at a time and Pathologists look at one slide at a time, but for some reason we like to batch and queue large numbers of slides in the IHC lab. Is quality important? Obviously it is. So why do we need to have highly skilled technologists overseeing the "automated" equipment? Lets be honest about our "automated" equipment, what we really have are glorified automated pipetting devices. Some of them provide the reagents, some we have to make up the reagents ourselves, some heat the slide some do not. IHC is not automated! If it were, the histotech cutting the section could place a bar-coded slide into a slot in the stainer as it was cut and the stainer would run the entire process, spitting out the completed slide onto the pathologists microscope where he/she would examine it. Slides would be delivered to the pathologist in a steady stream of 1 every couple of minutes, the process would be faster and the entire IHC lab would be eliminated. Oh wait, that would mean I would be out of work................... never mind (with apologies to SNL). Seriously though, the two largest cost drivers in IHC labs are the staff and the equipment/reagents. The people who have evolved in the labs generally like to tinker with their stains, "I can tweak it a bit to make it do this or that". In a perfect world, lab managers would pay big money for a machine that was, as described above, "truly automated". Technologists actually don't like not being able to tinker with a stain, add to that the costs for the reagents that are so much higher than our home made ones and it's no wonder we resist these pieces of equipment. They are not truly automated and they limit the technologists ability to prove their value in the lab. As an aside have you ever considered the markup on some of the polyclonal rabbit antibodies for human diagnostics. I have seen 100 uL of rabbit serum sell for hundreds of dollars, do your lab techs complain about these prices? Now, I realize that a true research lab operates under a different set of parameters and probably does require someone who can tweak a stain. These research labs are generally not high volume labs though, and realistically many could probably operate manually. It's the high volume labs that run 20-30 or more of the same stain every day that really need automation. What I hope will happen, is that two or more companies will embrace the ongoing revenue stream model currently used by Ventana, and then we should see a rapid evolution towards truly automated IHC stainers. Competition is always brings out the best in industry. Look at how clinical chemistry analyzers now are able to process serum samples, with little need for the technologist to fuss with the process. That should be the goal of the IHC stainer manufacturer. So what type of stainer should you choose? Well if you already have a good technologist or two in the lab and you have no opportunity reduce your personnel costs and if money is tight, you will likely choose an open system. My experience has been that open systems will lead to somewhat more variability in the results obtained, sometimes this can be good and sometimes it is bad. I have also seen that companies lose interest in product lines that no longer have a high profit potential, so beware of obsolescence. If high throughput and quick turn around time is critical, then I would recommend multiple units of the smallest volume stainer you can get (for more on this read up on LEAN thinking). If accessing or retaining highly skilled people are not in your future then you should be moving towards the "most automated" equipment like the Ventana machines. If you are a research lab with skilled people already in place then resist the move to automated equipment for as long as possible. There's just no sense in having the highest costs of both worlds. As Joe Nocito said earlier this week: Let the flaming begin.... Brian Chelack Prairie Diagnostic Services 2604-52 Campus Drive Saskatoon SK S7N 5B4 306-966-7241 From jnocito <@t> satx.rr.com Sun May 28 19:18:08 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sun May 28 19:18:15 2006 Subject: [Histonet] Ventana Benchmark References: <000701c6822c$73e0ebd0$eeeea8c0@SERVER01> Message-ID: <000a01c682b5$5d3e8e30$1bb30b43@yourxhtr8hvc4p> Bravo! Gudrun, Bravo! ----- Original Message ----- From: "Gudrun Lang" To: "Histonetliste (Histonetliste)" Sent: Sunday, May 28, 2006 2:58 AM Subject: AW: [Histonet] Ventana Benchmark First you are fascinated by the wonderful quality, go-away-system, easy to handle technique, fast results. Doctors see constant quality, less staff needed and therefore decrease of costs (?). And then, they want a special antibody to work - hmm. And then, the bill comes in. And now the complains go on. The technician, who is soo expensive, has to look for ways to make the system cheaper. Each sticker on the slides is counted, and each drop of antibody is saved. And there hangs the damokles-sword above our heads, that the next job-position will be shortened. "Make it cheaper - or your job is lost". It would have been better to evaluate the follow-costs before complaining, yes? I don't like the dependence on a single company - but I like the good staining-quality. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Joe Nocito Gesendet: Sonntag, 28. Mai 2006 00:59 An: Malcolm McCallum; Scott A. Ely; histonet@lists.utsouthwestern.edu Cc: histonet-owner@lists.utsouthwestern.edu Betreff: Re: [Histonet] Ventant Benchmark I'm not saying do it manually. I'm saying that there are less expensive ways to perform immunos than using Ventana's high cost reagents. Joe ----- Original Message ----- From: "Malcolm McCallum" To: "Joe Nocito" ; "Scott A. Ely" ; Cc: Sent: Saturday, May 27, 2006 10:39 AM Subject: RE: [Histonet] Ventant Benchmark Undoubtedly the cost and maintenance of automation is cheaper than the cost of additional employees salaries and benefits accompanied by associated duplication of manual systems. Additionally, equipment is depreciable, whereas human resources are a cost. VISIT THE JOURNAL HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-223-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Joe Nocito Sent: Sat 5/27/2006 8:29 AM To: Scott A. Ely; histonet@lists.utsouthwestern.edu Cc: histonet-owner@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventant Benchmark flaming time How come every one talks about the reliability and walk away technology, but no one ever talks about the price of running these machines? Shouldn't cost be a factor also? I've been in budget meetings all week. The only thing I heard was how expensive it is to run immunos. Yeah, we have two XTs, what do you expect? The opinions stated here are that of the author only and does not express the opinions of his employer, lawyers, siblings, significant others and their lawyers. Oh, by the way, I told the same thing to my Ventana Rep on Friday. He knows, so y'all don't need to call my CEO. Y'all know who you are. Let the flaming begin. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Scott A. Ely" To: Cc: Sent: Friday, May 26, 2006 11:58 AM Subject: [Histonet] Ventant Benchmark > We are considering buying some new equipment. I would like to know who > uses the Ventana Benchmark > immunostainer or other immunostainers and what you like and don't like > about it. > > Scott Ely, MD MPH > Section of Hematopathology > Department of Pathology > Weill Medical College of Cornell University > New York Presbyterian Hospital > 525 E. 68th Street > New York, NY 10021 > PH: 212-746-2442 > > Legal Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the original > intended recipient(s) selected by Dr. Ely and may contain confidential and > privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If you > are not the recipient specified by Dr. > Ely, please contact the sender by reply e-mail and destroy all copies of > the original message. > > ----- Original Message ----- > From: histonet-request@lists.utsouthwestern.edu > Date: Friday, May 26, 2006 12:29 pm > Subject: Histonet Digest, Vol 30, Issue 39 > > ---------------------------------------------------------------------------- ---- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ---------------------------------------------------------------------------- ---- > ---------------------------------------------------------------------------- ---- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.7.1/348 - Release Date: 5/25/2006 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.7.2/349 - Release Date: 5/26/2006 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.7.3/350 - Release Date: 5/28/2006 From v.weisbecker <@t> student.unsw.edu.au Sun May 28 19:36:31 2006 From: v.weisbecker <@t> student.unsw.edu.au (Vera Weisbecker) Date: Sun May 28 19:36:37 2006 Subject: [Histonet] Cartilage in CT-scans Message-ID: <005e01c682b7$ee3e3c80$0200a8c0@vera> Hello All, This is not stricly a histology question - but I was wondering whether anyone has experience with showing tissues other than bone in CT-scans? I am trying to visualize cartilage in micro-CT scans of formalin-fixed alcohol-preserved small young mammals. While cartilage doesn't show up "by itself", I wonder whether there is any staining agent the animals could be treated with to increase density of the cartilage. Any ideas? Thank you very much, Vera Vera Weisbecker Biological Sciences Building/Room 555 School of Biological, Earth and Environmental Sciences University of New South Wales UNSW/Sydney NSW 2052 Phone: +61 2 9385 2125 Fax: +61 2 9385 1558 E-mail: v.weisbecker@student.unsw.edu.au From jnocito <@t> satx.rr.com Sun May 28 19:37:25 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sun May 28 19:37:30 2006 Subject: [Histonet] Ventana Benchmark closed systems vs Open system stainers - a diatribe References: <000101c68299$066b2490$4f13e980@PDS11> Message-ID: <001001c682b8$0ea7f740$1bb30b43@yourxhtr8hvc4p> I too am from the "golden age" of the Fisher Code-On. That's way it took so may years to try the successor "Tek-mate 1000" Was it fully automated? No. You had to deparaffinize and retrieve off the instrument, fill the little trays with reagents, set up the programs and then it could be started, but you could rum multiple programs at the same time. Then one day, my Bio-Tek Solution's rep took me out to an expensive lunch just to tell me Ventana purchased Bio-Tek. Then, I learned that Ventana did such a price increase of the Tekmate's supplies, it was cost prohibited to use. okay, so now I have a bad taste in my mouth. Flash forward 10 years and now I have to XTs in my lab with 2 competement IHC techs. Now, we pay for reagents as well as knowledge. But, we can only have one run at a time on the machine. I know that there is software out there that can minipulate a machine at different intervals. Hell, my Sakura DRS 2000 does it, why not an immuno machine. If Ventana or any company for that matter, can allow me to perform more runs simultaneously, that it something I'll be willing to pay for. Not because one company works it's way down from the top. There, I've said my piece (or is it peace?) for now. As always, I shall over come and conquer. I am a legend in my own world, but they like me there. Joe ----- Original Message ----- From: "Brian Chelack" To: Sent: Sunday, May 28, 2006 3:55 PM Subject: [Histonet] Ventana Benchmark closed systems vs Open system stainers - a diatribe > It is interesting to note how firmly people plant themselves on one side > or > the other of the "Ventana Issues". I have worked with many different > immunostainers, going back to the old capillary gap Fisher Code-Ons. In my > estimation every company that makes a stainer has to make some sort of > compromise when it comes to its design. Every stainer has its own set of > strengths and weaknesses; I have yet to see one that is "perfect". As > technologists we all want to see the perfect machine that allows us to > load > individual slides whenever we want, process them completely, error free > and > automatically right down to the coverslip, and do it for next to nothing. > As > an added feature the stainer should allow all the IHC technologists to > maintain the "aura" of being highly skilled irreplaceable technical > artists > who can perform magic on paraffin sections. This means job security > because > department supervisors are certain their labs require the technologists > who > are busily tending to their "automated" machines. This "perfect" company > then must sell us one of these instruments and be prepared to walk away > from > the marketplace, because we technologists don't want to be "beholdin" to > them for any further purchases. We are mercenaries who will abandon them > in > an instant for the next company that offers us reagents for 3% cost > savings. > > So if I am this company what should I do? Why should I invest a great deal > of time and money into developing a stainer that provides me with no > chance > for an ongoing revenue stream? > > I have seen companies with good technology (for their times), fade away > due > to an inability to maintain a healthy cash flow. Patents are sold, other > companies try to adapt the technology to their product lines and life > staggers on. On the other hand, companies that keep their reagents > proprietary, and yes expensive, do have the money available to develop > their > product lines and look at new ideas. I think companies like Ventana, do > charge a lot of money for their reagents, but I also see how companies > like > Dako, with their new Artisan stainer is slowly moving towards generating > ongoing revenue streams. Watch for the convergence! > > Which one is the best choice? Well it depends. Every lab requires at > least > one person to turn on the lights and make coffee, so this person is also > available to perform IHC staining. Typically an IHC lab begins with > someone > doing the IHC staining by hand. If this person is at least moderately > skilled the lab becomes successful and requests for stains start arriving, > and arriving and arriving. Another skilled technologist is hired to no > avail, before long, both are swamped and begging for more help, but > budgets > are always tight so it must be time for an automated IHC stainer. What > should we choose? > > Well the ability to stain large numbers of slides is important, right? So > we > look at models that offer the ability to stain many slides at once, but > these models can only output their slides once or twice per day. This > means > you need to keep your two skilled technologists around in order to manage > the large bulges of work expelled by the stainers periodically throughout > the day. > > Is speed important? Well of course it is, but turn around times are > inversely proportional to the capacity of the equipment. Think about what > happens to the slide that arrives at the stainer 10 minutes after you hit > the start button, for that that matter, think about what happens to the > first slide that arrives at the stainer and then waits until enough other > slides are present to warrant starting the equipment. It is becoming > apparent that single piece flow is the most efficient method for improving > turn around times, but to my knowledge, all the equipment makers (Ventana > included, see the new BenchMark XT), believe that more slides in a batch > is > better. Histo techs cut one block at a time and Pathologists look at one > slide at a time, but for some reason we like to batch and queue large > numbers of slides in the IHC lab. > > Is quality important? Obviously it is. So why do we need to have highly > skilled technologists overseeing the "automated" equipment? Lets be honest > about our "automated" equipment, what we really have are glorified > automated > pipetting devices. Some of them provide the reagents, some we have to make > up the reagents ourselves, some heat the slide some do not. > > IHC is not automated! > > If it were, the histotech cutting the section could place a bar-coded > slide > into a slot in the stainer as it was cut and the stainer would run the > entire process, spitting out the completed slide onto the pathologists > microscope where he/she would examine it. Slides would be delivered to the > pathologist in a steady stream of 1 every couple of minutes, the process > would be faster and the entire IHC lab would be eliminated. Oh wait, that > would mean I would be out of work................... never mind (with > apologies to SNL). > > Seriously though, the two largest cost drivers in IHC labs are the staff > and > the equipment/reagents. The people who have evolved in the labs generally > like to tinker with their stains, "I can tweak it a bit to make it do this > or that". In a perfect world, lab managers would pay big money for a > machine > that was, as described above, "truly automated". Technologists actually > don't like not being able to tinker with a stain, add to that the costs > for > the reagents that are so much higher than our home made ones and it's no > wonder we resist these pieces of equipment. They are not truly automated > and > they limit the technologists ability to prove their value in the lab. As > an > aside have you ever considered the markup on some of the polyclonal rabbit > antibodies for human diagnostics. I have seen 100 uL of rabbit serum sell > for hundreds of dollars, do your lab techs complain about these prices? > > Now, I realize that a true research lab operates under a different set of > parameters and probably does require someone who can tweak a stain. These > research labs are generally not high volume labs though, and realistically > many could probably operate manually. It's the high volume labs that run > 20-30 or more of the same stain every day that really need automation. > > What I hope will happen, is that two or more companies will embrace the > ongoing revenue stream model currently used by Ventana, and then we should > see a rapid evolution towards truly automated IHC stainers. Competition is > always brings out the best in industry. Look at how clinical chemistry > analyzers now are able to process serum samples, with little need for the > technologist to fuss with the process. That should be the goal of the IHC > stainer manufacturer. > > So what type of stainer should you choose? Well if you already have a good > technologist or two in the lab and you have no opportunity reduce your > personnel costs and if money is tight, you will likely choose an open > system. My experience has been that open systems will lead to somewhat > more > variability in the results obtained, sometimes this can be good and > sometimes it is bad. I have also seen that companies lose interest in > product lines that no longer have a high profit potential, so beware of > obsolescence. If high throughput and quick turn around time is critical, > then I would recommend multiple units of the smallest volume stainer you > can > get (for more on this read up on LEAN thinking). If accessing or retaining > highly skilled people are not in your future then you should be moving > towards the "most automated" equipment like the Ventana machines. If you > are > a research lab with skilled people already in place then resist the move > to > automated equipment for as long as possible. There's just no sense in > having > the highest costs of both worlds. > > As Joe Nocito said earlier this week: > > Let the flaming begin.... > > > Brian Chelack > Prairie Diagnostic Services > 2604-52 Campus Drive > Saskatoon SK > S7N 5B4 > 306-966-7241 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.394 / Virus Database: 268.7.3/350 - Release Date: 5/28/2006 > > From zumbor <@t> email.cs.nsw.gov.au Mon May 29 02:27:35 2006 From: zumbor <@t> email.cs.nsw.gov.au (Rosalba) Date: Mon May 29 02:30:11 2006 Subject: [Histonet] Strange deposits Message-ID: <01C68345.2D44E630.zumbor@email.cs.nsw.gov.au> Hi All, We have a puzzling case. An optic nerve and muscle we received as a consult has strange colloid looking deposits. I have posted 2 pictures on histonet.org They are labelled AE H&E-0007 (H&E stain) and AE H&E-0008 (H&E polarised). Does anyone have any idea what they could be. The specimens had been fixed in alcohol. They show faint homogeneous staining with Alcian blue (pH 2.5) but no staining for calcium (alizarin red), phosphate or sulphate (von Kossa) or with toluidine blue, Congo red or PAS although they are faintly stained following pretreatment with diastase. They are also present in unstained frozen section of fixed optic nerve. Any clues Thanks Rosalba Zumbo Manager Histology Dept Department of Forensic Medicine 42-50 Parramatta Rd Glebe NSW 2037 Australia PH: 61 2 85847842 FAX: 61 2 95664573 "This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please destroy it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of the Sydney South West Area Health Service." From kmilne <@t> bccancer.bc.ca Mon May 29 10:31:51 2006 From: kmilne <@t> bccancer.bc.ca (Milne, Katy) Date: Mon May 29 10:32:03 2006 Subject: [Histonet] Paraffin tape-transfer systems and TMAs Message-ID: <07979E76B0869D4E8C9FE4AA9FC06578F00ED8@srvex03.phsabc.ehcnet.ca> Hi all, We started doing TMAs a little while ago and are looking for a way to maximize the number of samples that section nicely per slide. Sometimes we get compression of a few samples or a few just don't cut well. I admit I don't know much about tape-transfer systems but I'm wondering if there is anyone that does TMAs regularly that would be able to offer some insight as to whether having a tape transfer system dramatically increases the quality of slides from TMAs or not. Currently I'm just cutting directly from the TMA block using a regular rotary microtome. Thanks Katy Milne Research Assistant II Deeley Research Centre Vancouver Island Centre British Columbia Cancer Agency 2410 Lee Avenue Victoria BC V9A 4R2 From livieira <@t> ualg.pt Mon May 29 11:17:59 2006 From: livieira <@t> ualg.pt (Lina Vieira) Date: Mon May 29 11:20:23 2006 Subject: [Histonet] Methods for meiosis and mitosis Message-ID: <004b01c6833b$73e340a0$2914100a@labhistologia> Hi, I need to make some slides wich provide a good image of mitosis and meiosis for education purposes and I have only week to do it! Have You any suggestions? Protocols? Bibliography? Tanks in advance, Lina Vieira University of Algarve Portugal From ndumont <@t> neurochem.com Mon May 29 15:23:13 2006 From: ndumont <@t> neurochem.com (Dumont, Nancy) Date: Mon May 29 15:19:14 2006 Subject: [Histonet] anti-Abeta antibodies Message-ID: <1FAA5B74210C9A45A37A46EF6A969761076446@svrneurochem9.neurochem.local> Hello, I am looking for information on antibody affinity against Abeta peptide, at either the N-terminal part (such as 6E10 monoclonal antibody) or C terminal (such as polyclonal antibodies specific for A?40 or A?42 from Chemicon). Does anybody have some data sources to mention to me? I also have a question about pan-Abeta antibody. We constantly have cellular labeling with the pan-Abeta antibody from BioSource (sequence 15 to 30) when we process brain sections of transgenic mice. Does someone know if this antibody also labels APP? In fact, it seems that the labeling is indeed nuclear. Controls without primary or secondary antibodies are totally blank. A reference cited by the company also says that preabsorption of the antibody with the peptide leaded to the absence of any labeling. Thanks in advance for any advice! Nancy Dumont Research technician Neurochem Inc. Montreal, Canada L'information contenue dans ce courriel est confidentielle et prot?g?e par le secret professionnel. Elle n'est destin?e qu'? l'usage du destinataire indiqu? ci-dessus. Il est strictement interdit de distribuer ce document ou l'information qu'il contient. Si vous avez re?u ce courriel par erreur, ou s'il ne vous est pas destin?, veuillez le mentionner imm?diatement ? l'exp?diteur et d?truire tous les exemplaires de ce courriel. Merci. / This email is intended only for the party to whom it is addressed, and may contain information which is privileged or confidential. Any other disclosure is strictly prohibited. If you have received this email in error, or are not a named recipient, please notify the sender immediately and destroy all copies of this email. Thank you. From Barry.R.Rittman <@t> uth.tmc.edu Mon May 29 18:51:30 2006 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Mon May 29 18:53:46 2006 Subject: [Histonet] Methods for meiosis and mitosis Message-ID: Lina One of the simplest for mitosis is to use onion root tips, they grow rapidly and can process them via easilty. For meiosis can use mouse testis. Chromosomes easily seen using either Feulgen's reaction or iron hematoxylin. Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Lina Vieira Sent: Mon 5/29/2006 11:17 AM To: Histonet Subject: [Histonet] Methods for meiosis and mitosis Hi, I need to make some slides wich provide a good image of mitosis and meiosis for education purposes and I have only week to do it! Have You any suggestions? Protocols? Bibliography? Tanks in advance, Lina Vieira University of Algarve Portugal _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DDDeltour <@t> mar.med.navy.mil Tue May 30 05:19:32 2006 From: DDDeltour <@t> mar.med.navy.mil (Deltour, Douglas D. (HM2)) Date: Tue May 30 05:19:55 2006 Subject: [Histonet] To Ventana or not to Ventana Message-ID: <3F500F8B416C554EBB21FF16642F72E959CE9F@marxchg03.mar.med.navy.mil> I use two of the Benchmark XT's and love them. You could use the other open systems and cut down on cost per slide. You have to take into consideration your staffing. You can train other techs easily on the Benchmark. It would be much easier to rotate staff or make up for those sick days with the XT. If you tried to train them on an open system which includes making up buffers and so on, then it may be more of a headache. Good luck. Douglas Deltour HT(ASCP) -----Original Message----- From: Scott A. Ely [mailto:sae2001@med.cornell.edu] Sent: Friday, May 26, 2006 12:59 PM To: histonet@lists.utsouthwestern.edu Cc: histonet-owner@lists.utsouthwestern.edu Subject: [Histonet] Ventant Benchmark We are considering buying some new equipment. I would like to know who uses the Ventana Benchmark immunostainer or other immunostainers and what you like and don't like about it. Scott Ely, MD MPH Section of Hematopathology Department of Pathology Weill Medical College of Cornell University New York Presbyterian Hospital 525 E. 68th Street New York, NY 10021 PH: 212-746-2442 Legal Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the original intended recipient(s) selected by Dr. Ely and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the recipient specified by Dr. Ely, please contact the sender by reply e-mail and destroy all copies of the original message. ----- Original Message ----- From: histonet-request@lists.utsouthwestern.edu Date: Friday, May 26, 2006 12:29 pm Subject: Histonet Digest, Vol 30, Issue 39 From Julie.Sanders <@t> va.gov Tue May 30 06:06:39 2006 From: Julie.Sanders <@t> va.gov (Sanders, Julie, VHACIN) Date: Tue May 30 06:06:45 2006 Subject: [Histonet] Ventana Benchmark In-Reply-To: <526hb8$sc4pa@mtasac2.sac.va.gov> Message-ID: <2D4ACE41DEFE93428F23D77988EFBCB50E5FEA@VHAV10MSGA2.v10.med.va.gov> I agree with Joe, the cost of running the Ventana is high. My chief is always trying to figure out a way for us to not do immunos in house just because of this. With that said, our Ventana sales rep has given us the best prices possible for the volume we run, which is not that high compared to large hospitals/labs. I wish there were some way to keep the cost down for running this particular machine (we have a Benchmark XT), but there isn't and costs can only go up, as they do every year. However, we like Ventana, the technology, tech support, and sales support. Our pathologists would walk if we ever stopped using it as it has provided them with the best, most reliable technology we have found on the market. "How come every one talks about the reliability and walk away technology, but no one ever talks about the price of running these machines? Shouldn't cost be a factor also? I've been in budget meetings all week. The only thing I heard was how expensive it is to run immunos." Julie Sanders, BA, HT(ASCP) Supervisor, Anatomic Pathology Cincinnati VAMC From Jacqueline.Malam <@t> rli.mbht.nhs.uk Tue May 30 06:12:37 2006 From: Jacqueline.Malam <@t> rli.mbht.nhs.uk (Malam Jacqueline) Date: Tue May 30 06:14:04 2006 Subject: [Histonet] Ventana Benchmark XT Message-ID: We have used a Benchmark XT for two and a half years now and it's been great. Advantages - complete standardisation - a noticeable saving in time as the antigen retrieval is automatic - 3 primary antisera incubation temperatures (it is interesting to discover that some like it at room temp and some like it hot!) - the variable antigen retrievals, primary incubation times and temperatures give plenty of choices for optimisation - making antisera up in "bulk" in prep kit dispensers saves you having to make them up each time with some waste and, with some antisera working at higher dilutions, this can lead to savings - prompt servicing, excellent help over the phone if you have to do some minor repairs or problem solving - even if you are not mechanically minded - and their English puts me to shame! - prompt dispatch of consumable orders - good training course in Strasbourg Disadvantages - routine maintenance can be a bit involved; e.g. you have to dismantle some of it to do a vortex mix check each month and it needs a decontamination every 3 months - water quality must be good - you don't want water tanks that aren't cleaned out annually and you need a decent de-ioniser or you can get micro-organisms getting through into the XT and furring up its arteries. You then have to sterilise all containers (bulk and those on the XT) with hypochlorite bleach before making or refilling with fresh buffer - it doesn't like some antisera clones like the oestrogen receptor clone 1D5; then again, some clones that didn't work too well manually or on other systems work well on the XT - just be aware when you are optimising if it doesn't work very well - try another clone - it may have a rather quiet alarm. Nothing could be done about it so, as our XT is in another room, we use a baby monitor Hope this helps Jacqui Malam Lancaster Infirmary UK DISCLAIMER: This e-mail is confidential and privileged. If you are not the intended recipient please accept our apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform postmaster@rli.mbht.nhs.uk that this message has gone astray before deleting it. Comments or opinions expressed in this email are those of their respective contributors only. The views expressed do not represent the views of the Trust, its management or employees. University Hospitals of Morecambe Bay NHS Trust is not responsible and disclaims any and all liability for the content of comments written within.Thank you for your co-operation. From gayle.brosnanwatters <@t> sru.edu Tue May 30 06:32:07 2006 From: gayle.brosnanwatters <@t> sru.edu (gayle brosnanwatters) Date: Tue May 30 06:32:13 2006 Subject: [Histonet] question Message-ID: <8172F14C39FCAF4E99E266F6756A3683076F9653@msfexch01.srunet.sruad.edu> Dear Listers, I don't know if this is an appropriate question, so please forgive me if it is not. I have decided to retire from academia at the end of the school year. I am a psychology professor, but I have been doing research in a small way, and you folks have been an enormous help several times. About 6 years ago I ended up owning a brand new Leica sliding microtome - I believe the model is the SM2000R. (It was a fluke - a retiring colleague shipped his old freezing microtome to me, the shipper dropped it, it was insured for full value, so I got this for "free." I have the receipt). I know that it is identical to the one they sell today for about 10K, which is what I paid for it. I did not buy the extra freezing part, but used it with an attachment that let me use dry ice for frozen sectioning. Anyway, I would like to sell it. Can anyone tell me where I could go to do so? It was only used about 10 times, as I ended up using my old ultramicrotome for all the work I've done. Someone told me that I should expect to get no less than 5K, and because it is in such good condition, and it is identical to the new ones, maybe up to 7. Does anyone have any suggestions? Again, I hope this is an appropriate question. I'd appreciate any help you can give me. Gayle L. Brosnan-Watters, PhD Assistant Professor Dept of Psychology Treasurer, Faculty for Undergraduate Neuroscience 226 Vincent Science Hall Slippery Rock University Slippery Rock, PA 16057 gayle.brosnanwatters@sru.edu 724-738-2529 - office 724-738-4807 - fax From Jackie.O'Connor <@t> abbott.com Tue May 30 06:36:22 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Tue May 30 06:36:50 2006 Subject: [Histonet] whole villi In-Reply-To: Message-ID: I process mouse GI routinely looking for Caspase 3 in the villi - We cut short lengths of small intestine, open it, and place it (lumen up) on bilbous paper which has been dipped in fixative. The segments stay on the paper all the way through paraffin processing. To embed, I remove the segments off the paper, and easily embed multiple segments on edge in one block. The villi remain undamaged. Jackie O' "Rittman, Barry R" Sent by: histonet-bounces@lists.utsouthwestern.edu 05/26/2006 10:36 AM To cc Subject RE: [Histonet] whole villi Renee Do you need to embed and cut them as a circle? If not I would suggest that you slit along the length and then place the connective tissue (or muscle) side down on a piece of card. The pieces should stick to the card. Then fix, remove from card and process as normal. Once they are fixed they should remain flat. If you have really big pieces can pin these to a piece of cork and float tissue side down in the fixative - pin using stainless steel pins, plastic pins or hedgehog quills. You will always have trouble in getting sections with entire lengths of villi in the jejunum because of the long length of these villi in this region. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Till, Renee Sent: Friday, May 26, 2006 10:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] whole villi I sometimes work with rat and mouse gi tissues, and as you might expect one of the most time consuming things is getting the tissues embedded good. The colon has not been as much of a problem, but lately we are doing more with the jejunum and getting whole villi is enough to make me scream. It seems to me that one of my main problems is that sometime between the collection of the tissues (which is not done by me) and when I embed them, the tissues often curl up and it is hard to get a good straight piece to embed. We embed them on end? (so when cut it makes a little O). Any ideas on how to keep them straight during the processing? Or maybe if there is something else that I am not thinking of that could be a problem. Thanks. Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72002 Office (501)364-2785 Fax (501)364-3161 ======================================================================== ======================== Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ======================================================================== ======================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Tue May 30 06:55:17 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Tue May 30 06:55:56 2006 Subject: [Histonet] Ventana Benchmark In-Reply-To: <001d01c681e0$cc3c0220$1bb30b43@yourxhtr8hvc4p> Message-ID: Anybody been a patient in a hospital recently? I was - and NO I'm not on Medicare - I have really good insurance from my husband's plan at Motorola. I was in the hospital for 4 days - my bill was over $20K - the negotiated payment was about 15% of the actual billed amount. I don't understand how hospitals are managing to stay open only recovering 10% of the actual cost of services. Seems our economy is driven by the insurance companies. "Joe Nocito" Sent by: histonet-bounces@lists.utsouthwestern.edu 05/27/2006 05:56 PM To , "'Scott A. Ely'" , cc histonet-owner@lists.utsouthwestern.edu Subject Re: [Histonet] Ventant Benchmark Patsy, we charge what Medicare pays. Many insurance companies pay less. This is what fries my cookies. These people want to make money, yet pays trough the nose for immunos. Penny wise, dollar foolish is what my father would say. Joe ----- Original Message ----- From: To: "'Joe Nocito'" ; "'Scott A. Ely'" ; Cc: Sent: Saturday, May 27, 2006 10:25 AM Subject: RE: [Histonet] Ventant Benchmark >I am with you Joe. > I have not ever been able to understand how the labs can justify spending > so > much to run IHC, I guess they just pass the cost on to the patient, I can > do > the same thing for about 1/100 of the cost of running an instrument like > the > Ventana using an open system like the Dakoautostainer and making up a lot > of > my own reagents like the buffers. I work in research and don't have a > money > cow like the patient health care system to milk. No wonder health care > costs are so,years ago when I worked in clinical we used to do what ever > we > could to try and help save the patient expense, sure doesn't seem like > that > is the case anymore. I know that understaffing, quick turn around and > untrained personnel is a big part of the problem here but come on, do you > really want someone who only knows how to apply a bar code to be running > your diagnostic IHC's? > Bring on the flaming...... > Patsy > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito > Sent: Saturday, May 27, 2006 7:29 AM > To: Scott A. Ely; histonet@lists.utsouthwestern.edu > Cc: histonet-owner@lists.utsouthwestern.edu > Subject: Re: [Histonet] Ventant Benchmark > > flaming time > How come every one talks about the reliability and walk away technology, > but > > no one ever talks about the price of running these machines? Shouldn't > cost > be a factor also? > I've been in budget meetings all week. The only thing I heard was how > expensive it is to run immunos. Yeah, we have two XTs, what do you expect? > > The opinions stated here are that of the author only and does not express > the opinions of his employer, lawyers, siblings, significant others and > their lawyers. > > Oh, by the way, I told the same thing to my Ventana Rep on Friday. He > knows, > > so y'all don't need to call my CEO. Y'all know who you are. > > Let the flaming begin. > > Joe Nocito BS, HT(ASCP)QIHC > Histology Manager > Pathology Reference Lab > San Antonio, TX > ----- Original Message ----- > From: "Scott A. Ely" > To: > Cc: > Sent: Friday, May 26, 2006 11:58 AM > Subject: [Histonet] Ventant Benchmark > > >> We are considering buying some new equipment. I would like to know who >> uses the Ventana Benchmark >> immunostainer or other immunostainers and what you like and don't like >> about it. >> >> Scott Ely, MD MPH >> Section of Hematopathology >> Department of Pathology >> Weill Medical College of Cornell University >> New York Presbyterian Hospital >> 525 E. 68th Street >> New York, NY 10021 >> PH: 212-746-2442 >> >> Legal Confidentiality Notice: This e-mail message, including any >> attachments, is for the sole use of the original >> intended recipient(s) selected by Dr. Ely and may contain confidential >> and > >> privileged information. Any >> unauthorized review, use, disclosure or distribution is prohibited. If >> you > >> are not the recipient specified by Dr. >> Ely, please contact the sender by reply e-mail and destroy all copies of >> the original message. >> >> ----- Original Message ----- >> From: histonet-request@lists.utsouthwestern.edu >> Date: Friday, May 26, 2006 12:29 pm >> Subject: Histonet Digest, Vol 30, Issue 39 >> >> > > > ---------------------------------------------------------------------------- > ---- > > >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > ---------------------------------------------------------------------------- > ---- > > >> > > > ---------------------------------------------------------------------------- > ---- > > > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.394 / Virus Database: 268.7.1/348 - Release Date: 5/25/2006 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.394 / Virus Database: 268.7.2/349 - Release Date: 5/26/2006 > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RitaR <@t> lexhealth.org Tue May 30 08:00:39 2006 From: RitaR <@t> lexhealth.org (Rita Riddle) Date: Tue May 30 07:56:36 2006 Subject: [Histonet] Energy Beam Message-ID: Can someone give me the number to Energy Beam Sciences Inc. . Thanks Rita Riddle HT(ASCP) Lead Histology Tech Lexington Medical Center West Columbia,SC 29169 803-791-2881 _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. From JWEEMS <@t> sjha.org Tue May 30 08:05:17 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue May 30 08:04:51 2006 Subject: [Histonet] Energy Beam Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202736C77@sjhaexc02.sjha.org> 800-992-9037 or 860-653-0411 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rita Riddle Sent: Tuesday, May 30, 2006 9:01 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Energy Beam Can someone give me the number to Energy Beam Sciences Inc. . Thanks Rita Riddle HT(ASCP) Lead Histology Tech Lexington Medical Center West Columbia,SC 29169 803-791-2881 _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From la.sebree <@t> hosp.wisc.edu Tue May 30 08:13:20 2006 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Tue May 30 08:13:29 2006 Subject: [Histonet] Ventant Benchmark Message-ID: We also titer a number of our antibodies, especially those infrequently ordered. These we don't put in Ventana dispensers but rather manually apply them as all the VMS instruments allow one to do. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Sunday, May 28, 2006 12:35 PM To: Joe Nocito; Malcolm McCallum; Scott A. Ely; histonet@lists.utsouthwestern.edu Cc: histonet-owner@lists.utsouthwestern.edu Subject: RE: [Histonet] Ventant Benchmark That's what I was saying too. Even with all our cost cutting attempts, do enough immunos to support 3 reagent-leased DAKO instruments. They're walk away too = after you get them loaded anyway! - and the reagents are not nearly as expensive. We titer all the antibodies that are available - a savings over the prepared Abs. The company has been a great partner for our laboratory. j Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Joe Nocito Sent: Sat 5/27/2006 6:58 PM To: Malcolm McCallum; Scott A. Ely; histonet@lists.utsouthwestern.edu Cc: histonet-owner@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventant Benchmark I'm not saying do it manually. I'm saying that there are less expensive ways to perform immunos than using Ventana's high cost reagents. Joe ----- Original Message ----- From: "Malcolm McCallum" To: "Joe Nocito" ; "Scott A. Ely" ; Cc: Sent: Saturday, May 27, 2006 10:39 AM Subject: RE: [Histonet] Ventant Benchmark Undoubtedly the cost and maintenance of automation is cheaper than the cost of additional employees salaries and benefits accompanied by associated duplication of manual systems. Additionally, equipment is depreciable, whereas human resources are a cost. VISIT THE JOURNAL HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-223-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Joe Nocito Sent: Sat 5/27/2006 8:29 AM To: Scott A. Ely; histonet@lists.utsouthwestern.edu Cc: histonet-owner@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventant Benchmark flaming time How come every one talks about the reliability and walk away technology, but no one ever talks about the price of running these machines? Shouldn't cost be a factor also? I've been in budget meetings all week. The only thing I heard was how expensive it is to run immunos. Yeah, we have two XTs, what do you expect? The opinions stated here are that of the author only and does not express the opinions of his employer, lawyers, siblings, significant others and their lawyers. Oh, by the way, I told the same thing to my Ventana Rep on Friday. He knows, so y'all don't need to call my CEO. Y'all know who you are. Let the flaming begin. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Scott A. Ely" To: Cc: Sent: Friday, May 26, 2006 11:58 AM Subject: [Histonet] Ventant Benchmark > We are considering buying some new equipment. I would like to know who > uses the Ventana Benchmark > immunostainer or other immunostainers and what you like and don't like > about it. > > Scott Ely, MD MPH > Section of Hematopathology > Department of Pathology > Weill Medical College of Cornell University > New York Presbyterian Hospital > 525 E. 68th Street > New York, NY 10021 > PH: 212-746-2442 > > Legal Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the original > intended recipient(s) selected by Dr. Ely and may contain confidential and > privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If you > are not the recipient specified by Dr. > Ely, please contact the sender by reply e-mail and destroy all copies of > the original message. > > ----- Original Message ----- > From: histonet-request@lists.utsouthwestern.edu > Date: Friday, May 26, 2006 12:29 pm > Subject: Histonet Digest, Vol 30, Issue 39 > > ------------------------------------------------------------------------ -------- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------------------------------------------------ -------- > ------------------------------------------------------------------------ -------- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.7.1/348 - Release Date: 5/25/2006 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.7.2/349 - Release Date: 5/26/2006 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From rhrogers <@t> iupui.edu Tue May 30 09:37:48 2006 From: rhrogers <@t> iupui.edu (Rogers, Rhonda) Date: Tue May 30 09:37:56 2006 Subject: [Histonet] Cre, eGFP, and B-Gal Antibodies Message-ID: <6CB660A4F5C2D441961387F9CF60EBCA0C74A9@iu-mssg-mbx01.exchange.iu.edu> Good morning, I will soon try Cre (from Novagen), eGFP (from Molecular Probes), and B-Gal (also from Molecular Probes) rabbit antibodies on formalin-fixed paraffin-embedded sections and/or frozen sections of mice embryos. Is anyone already using these antibodies for IHC staining and, if so, would you be willing to share your protocols? Thanks, Rhonda From katherine-walters <@t> uiowa.edu Tue May 30 10:17:28 2006 From: katherine-walters <@t> uiowa.edu (Walters, Katherine S) Date: Tue May 30 10:17:38 2006 Subject: [Histonet] slow draining paraffin dispenser Message-ID: Hi, I have Tissue Tek paraffin dispersing console Model 4586 (older model). It has worked well for many years, but lately it appears there is a partial clog, as it is dispensing much slower. Are there any (safe) protocols for clearing out these tubes? From mcauliff <@t> umdnj.edu Tue May 30 10:25:44 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue May 30 10:25:29 2006 Subject: [Histonet]Hospital costs/new topic In-Reply-To: References: Message-ID: <447C63F8.4030903@umdnj.edu> Hospitals don't manage "to stay open only recovering 10% of the actual cost of services." That would be impossible. They overbill to cover the costs of indigent care, improvements, etc. The only people who pay full price are the uninsured who have no one to negotiate for them. Geoff Jackie M O'Connor wrote: >Anybody been a patient in a hospital recently? I was - and NO I'm not on >Medicare - I have really good insurance from my husband's plan at >Motorola. >I was in the hospital for 4 days - my bill was over $20K - the negotiated >payment was about 15% of the actual billed amount. I don't understand >how hospitals are managing to stay open only recovering 10% of the actual >cost of services. Seems our economy is driven by the insurance >companies. > > > > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From cfavara <@t> niaid.nih.gov Tue May 30 10:42:32 2006 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID) [E]) Date: Tue May 30 10:42:38 2006 Subject: [Histonet] slow draining paraffin dispenser In-Reply-To: Message-ID: Hot hair dryer might help! c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives -----Original Message----- From: Walters, Katherine S [mailto:katherine-walters@uiowa.edu] Sent: Tuesday, May 30, 2006 8:17 AM To: histonet@pathology.swmed.edu Subject: [Histonet] slow draining paraffin dispenser Hi, I have Tissue Tek paraffin dispersing console Model 4586 (older model). It has worked well for many years, but lately it appears there is a partial clog, as it is dispensing much slower. Are there any (safe) protocols for clearing out these tubes? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Tue May 30 10:56:33 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Tue May 30 10:58:09 2006 Subject: [Histonet] slow draining paraffin dispenser References: Message-ID: Try cleaning out the filter disk near the bottom, inside the tank. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Walters, Katherine S Sent: Tue 5/30/2006 11:17 AM To: histonet@pathology.swmed.edu Subject: [Histonet] slow draining paraffin dispenser Hi, I have Tissue Tek paraffin dispersing console Model 4586 (older model). It has worked well for many years, but lately it appears there is a partial clog, as it is dispensing much slower. Are there any (safe) protocols for clearing out these tubes? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Tue May 30 11:04:17 2006 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Tue May 30 11:04:24 2006 Subject: [Histonet] floaters Message-ID: <0E394B648E5284478A6CCB78E5AFDA270171FDDB@hpes1.HealthPartners.int> How does everyone handle their embedding techniques in regard to contaminating one tissue with another? We are doing here what I have done at the other lab I worked at and lately the pathologist is seeing a few "floaters" on a slide where they shouldn't be!! Just thought I would like to throw this out for discussion and hopefully come away with a technique better than ours!! Thanks, as always!!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From blackmail <@t> intermail.co.za Thu May 25 11:15:53 2006 From: blackmail <@t> intermail.co.za (black) Date: Tue May 30 11:09:53 2006 Subject: [Histonet] Stent paper and Histonet connection Message-ID: <000701c680a3$a0c41f30$6725d0c4@yourdbba124d3a> To all Histonetters I just thought I should mention. that the stent paper would never have come about, if it had not been for the connections made through Histonet. This is such a valuable tool for sharing of knowledge and technical skills, and for net working with colleagues world wide, using similar technology. For this paper to have become a reality, the connection was made via histonet, which resulted in travel to Ottawa from South Africa and visa versa, as well as a poster in Savannah, Georgia. There are some extremely knowledgable people who regularly contribute to this network, to mention a few...Gayle Kallis, Chris V D Loos, John Kiernan etc.etc...and many more. WWe are very fortunate to have this technology at our disposal. Thanks to Histonet for keeping us all up to date!! Keep it up Melanie Black. From bosborn <@t> health.usf.edu Fri May 26 15:05:37 2006 From: bosborn <@t> health.usf.edu (Osborn, Barbara) Date: Tue May 30 11:09:56 2006 Subject: [Histonet] Re: methyl green counterstain Message-ID: <841D767DCDE87C49A02DA6DFC0BABABFA71F21@COMEXCHANGE.hscnet.hsc.usf.edu> I believe it's the antigen retrieval that affects the methyl green counterstain. We purchase our methyl green from Vector Labs and although the staining is lighter than sections with no antigen retrieval, it does not fade out during dehydration. We counterstain for 10 min at 60oC with decent results. Barbara Osborn University of South Florida Message: 6 Date: Thu, 25 May 2006 17:50:03 -0400 From: "Jacqui Detmar" Subject: [Histonet] methyl green counterstain To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi all. I am doing some immunohistochemistry and TUNEL staining on mouse placental tissue. I have noticed that my methyl green counterstain on placentae assayed for TUNEL looks normal, but when I apply the same steps to tissue from the same block, but exposed to immunohistochemistry, the methyl green counterstain is very weak, bleaches out quickly during dehydration and generally looks pretty crappy. The methyl green recipe I am using is as follows: 0.5% methyl green in 0.1M sodium acetate buffer, pH 4.2. I would like to emphasize that no matter how quickly I dehydrate with the IHC sections, the colour is still very dim. The reason I am using methyl green is b/c I am doing IHC for nuclear proteins (Ki67, etc.) and don't want to use hematoxylin, since it's a little dark. Any suggestions? Thanks, Jacqui Detmar From tpmorken <@t> labvision.com Tue May 30 11:33:40 2006 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Tue May 30 11:33:53 2006 Subject: [Histonet] Ventana Benchmark closed systems vs Open system st ainers - a diatribe Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA20328D95C@usca0082k08.labvision.apogent.com> Wow Brian, Spring must have hit the Great White North! The sap is starting to run! Brian wrote (among other things): "As technologists we all want to see the perfect machine that allows us to load individual slides whenever we want, process them completely, error free and automatically right down to the coverslip, and do it for next to nothing. " Brian, you forgot the imaging system! The "Automated IHC System" also has to deliver the coverslipped slide to an imaging system that scans the entire tissue section to produce a Virtual Slide. (preferably at 40X and 10 focus levels, and then saves it (all 800 GB) to a server so anyone, anywhere (India?) can do the pathology consult (don't laugh, this is now happening in radiology)). We've actually heard this from customers. Lets, just say, yes, technically it can be done. But, few, if any, labs would have the half-million (US), or more, to buy the instrument. And you say you want it on reagent rental? You'd have to do thousands of slides a day to qualify. Maybe all the labs will eventually be consolidated into a few big labs and it will be possible. Not any time soon, though. Brian also wrote: "What I hope will happen, is that two or more companies will embrace the ongoing revenue stream model currently used by Ventana, and then we should see a rapid evolution towards truly automated IHC stainers. Competition is always brings out the best in industry. Look at how clinical chemistry analyzers now are able to process serum samples, with little need for the technologist to fuss with the process. That should be the goal of the IHC stainer manufacturer. " The dream of a totally automated IHC system for fixed tissue sections is not at all as simple as a chemistry or blood analysing system. In the clinical lab they are dealing with FRESH blood or fluid samples, all taken in standardized ways. The samples are nearly identical in every case. So a company is much more likely to be able to create instruments and reagents that work well over a broad range of labs. The big wrench in the IHC system is that no two labs have the same tissue procurement, fixation and processing system. Dr. Clive Taylor, the guru of standardization, has almost given up his crusade in convincing all labs to follow standardized procedures. For all their talk of wanting "the best" for their patients, no two pathologists can agree on what is "the best." Even the advent of "antigen retrieval" which was to overcome the fixation/processing variation problem, has itself become a complicated variable (with a slew of attendant instruments). So, considering infinite variation in tissue fixation and processing, AR and staining, it is nearly impossible to simply give a batch of reagents and an instrument to any given lab and have them all work perfectly from day one. In fact, every single antibody and instrument company in the business employs a cadre of Quality Control technologists who labor to be sure the antibodies sold are optimized and indeed "working," and they also employ a legion of "technical reps" who bravely go into unfamiliar labs and atttempt to "optimize" all the reagents, antibodies and protocols so that the system "works" in that lab. Believe me, there are very few labs that can do this successfully without some help. Besides that, do histotechs really want to be like Med Techs who just sit around looking at printouts all day long? Maybe not. We just like fiddling with things. Tim Morken Lab Vision - Neomarkers www.labvision.com Free webhosting for US State Histotechnology Societies: http://www.labvisioncorp.com/demowebsite/index.cfm -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brian Chelack Sent: Sunday, May 28, 2006 1:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana Benchmark closed systems vs Open system stainers - a diatribe Etc. Brian Chelack Prairie Diagnostic Services 2604-52 Campus Drive Saskatoon SK S7N 5B4 306-966-7241 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue May 30 11:43:25 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue May 30 11:43:29 2006 Subject: [Histonet] floaters In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA270171FDDB@hpes1.HealthPartners.int> Message-ID: <20060530164325.8085.qmail@web61219.mail.yahoo.com> Dorothy: First try to find the source of the floater. Compare the stained slide containing the floater with the block surface. If the tissue seen as floater is not in the block, it was picked up in the water bath. The solution is to clean the surface of the water with a tissue after each block is cut. If you can identify in the block the source of the floater it means that contamination occurred either during cassetting or during embedding. Make sure that the cassetting is done in a way that prevents carry-on of small pieces of tissue. The embedding center (the heating wells for the forceps) have to be empied with a pipette once embedding is finished. After that use a Q-tip to completely dry-clean the wells. Forceps should be cleaned after each block is embedded. Care is the solution and attention to detail is the only solution. Hope this will help you. Ren? J. "Webb, Dorothy L" wrote: How does everyone handle their embedding techniques in regard to contaminating one tissue with another? We are doing here what I have done at the other lab I worked at and lately the pathologist is seeing a few "floaters" on a slide where they shouldn't be!! Just thought I would like to throw this out for discussion and hopefully come away with a technique better than ours!! Thanks, as always!!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Feel free to call! Free PC-to-PC calls. Low rates on PC-to-Phone. Get Yahoo! Messenger with Voice From PMonfils <@t> Lifespan.org Tue May 30 12:03:30 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Tue May 30 12:03:37 2006 Subject: [Histonet] floaters Message-ID: <09C945920A6B654199F7A58A1D7D1FDE0171771B@lsexch.lsmaster.lifespan.org> Keep in mind that such "floaters" can be due either to embedding technique or to sectioning technique. If the same "floater" appears in two successive sections, then it was a contaminant in the block at the time of embedding; but if it appears in only one section it probably got on the slide from the water bath during sectioning. If the pathologist has only one section on the slide then it may be impossible to tell where the floater came from, unless the unwanted bit of tissue can still be seen in the block face. Cleanliness is the means of preventing both types of contamination. The hot plate of the embedding unit should be wiped frequently with some sort of absorbent wipes, especially after handling any specimen which is friable or which consists of multiple small fragments. And, the surface of the water bath should likewise be "swept" frequently with a similar absorbent wipe. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Webb, > Dorothy L > Sent: Tuesday, May 30, 2006 9:04 AM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] floaters > > How does everyone handle their embedding techniques in regard to > contaminating one tissue with another? We are doing here what I have > done at the other lab I worked at and lately the pathologist is seeing a > few "floaters" on a slide where they shouldn't be!! Just thought I > would like to throw this out for discussion and hopefully come away with > a technique better than ours!! Thanks, as always!!! > ________________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please be > advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is strictly > prohibited. > > If you have received this e-mail in error, please immediately notify the > HealthPartners Support Center by telephone at (952) 967-6600. You will be > reimbursed for reasonable costs incurred in notifying us. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From gu.lang <@t> gmx.at Tue May 30 12:08:31 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue May 30 12:08:43 2006 Subject: AW: [Histonet] Ventana Benchmark closed systems vs Open system stainers - a diatribe In-Reply-To: <0556BE8AC5551E4E8AF6BB9E42509BA20328D95C@usca0082k08.labvision.apogent.com> Message-ID: <000c01c6840b$b1cdf790$eeeea8c0@SERVER01> Tim Morken asked, if we want the total automation in histotechnology. Definitly not. And for me it is even a little bit frightening, that research and knowledge is only performed by the companies. The technician is the one, who should know, how to make "histo" going. I don't like a future, when we will be only "on job trained costumers" and the best known thing is the support-hotline. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Morken, Tim - Labvision Gesendet: Dienstag, 30. Mai 2006 18:34 An: histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] Ventana Benchmark closed systems vs Open system stainers - a diatribe Wow Brian, Spring must have hit the Great White North! The sap is starting to run! Brian wrote (among other things): "As technologists we all want to see the perfect machine that allows us to load individual slides whenever we want, process them completely, error free and automatically right down to the coverslip, and do it for next to nothing. " Brian, you forgot the imaging system! The "Automated IHC System" also has to deliver the coverslipped slide to an imaging system that scans the entire tissue section to produce a Virtual Slide. (preferably at 40X and 10 focus levels, and then saves it (all 800 GB) to a server so anyone, anywhere (India?) can do the pathology consult (don't laugh, this is now happening in radiology)). We've actually heard this from customers. Lets, just say, yes, technically it can be done. But, few, if any, labs would have the half-million (US), or more, to buy the instrument. And you say you want it on reagent rental? You'd have to do thousands of slides a day to qualify. Maybe all the labs will eventually be consolidated into a few big labs and it will be possible. Not any time soon, though. Brian also wrote: "What I hope will happen, is that two or more companies will embrace the ongoing revenue stream model currently used by Ventana, and then we should see a rapid evolution towards truly automated IHC stainers. Competition is always brings out the best in industry. Look at how clinical chemistry analyzers now are able to process serum samples, with little need for the technologist to fuss with the process. That should be the goal of the IHC stainer manufacturer. " The dream of a totally automated IHC system for fixed tissue sections is not at all as simple as a chemistry or blood analysing system. In the clinical lab they are dealing with FRESH blood or fluid samples, all taken in standardized ways. The samples are nearly identical in every case. So a company is much more likely to be able to create instruments and reagents that work well over a broad range of labs. The big wrench in the IHC system is that no two labs have the same tissue procurement, fixation and processing system. Dr. Clive Taylor, the guru of standardization, has almost given up his crusade in convincing all labs to follow standardized procedures. For all their talk of wanting "the best" for their patients, no two pathologists can agree on what is "the best." Even the advent of "antigen retrieval" which was to overcome the fixation/processing variation problem, has itself become a complicated variable (with a slew of attendant instruments). So, considering infinite variation in tissue fixation and processing, AR and staining, it is nearly impossible to simply give a batch of reagents and an instrument to any given lab and have them all work perfectly from day one. In fact, every single antibody and instrument company in the business employs a cadre of Quality Control technologists who labor to be sure the antibodies sold are optimized and indeed "working," and they also employ a legion of "technical reps" who bravely go into unfamiliar labs and atttempt to "optimize" all the reagents, antibodies and protocols so that the system "works" in that lab. Believe me, there are very few labs that can do this successfully without some help. Besides that, do histotechs really want to be like Med Techs who just sit around looking at printouts all day long? Maybe not. We just like fiddling with things. Tim Morken Lab Vision - Neomarkers www.labvision.com Free webhosting for US State Histotechnology Societies: http://www.labvisioncorp.com/demowebsite/index.cfm -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brian Chelack Sent: Sunday, May 28, 2006 1:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana Benchmark closed systems vs Open system stainers - a diatribe Etc. Brian Chelack Prairie Diagnostic Services 2604-52 Campus Drive Saskatoon SK S7N 5B4 306-966-7241 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jodiputnam <@t> aol.com Tue May 30 12:28:09 2006 From: Jodiputnam <@t> aol.com (Jodiputnam@aol.com) Date: Tue May 30 12:28:27 2006 Subject: [Histonet]Hospital costs/new topic Message-ID: <3cb.2ec570b.31addaa9@aol.com> I had to write when I read the post on hospital charges. A few months agao, my husband was having what I was pretty certain was appendix pain so we went in to the ER. After they established that he did in fact have a very ripe appendix that needed to come out, we were in the ER for over 8 hours. He was admitted after he came out of recovery and was there for ~27 hours. I always like to take a look at the itemized bill after you are released. Its rather amusing. There were so many charges for the nurses coming in and changing his bandages and bedding and I did most of that myself. Sometimes I had to call and practically beg them to come and change the bedding do drainage etc. They rarely came by except on scheduled rounds. We had to empty his drain tube, etc. If I had a spare IV bag laying around, I would have changed that too since the alarm wouldnt shut off (alarm silence button was broken). I asked if we could have some items to give him a sponge bath so he would feel a little more clean, get the betadine and other stuff off. They were real quick to inform me that that was MY job not theirs. Times have changes. Long story short, unruptured appendix , 1 day in the hospital all total ~30k. They said that if it had ruptured it would have been closer to 5-7 days and we dont even want to think about that. I was so thankful to have insurance. We only ended up paying a few thousand dollars. Jodi From a_marcuzzi <@t> cantv.net Tue May 30 12:32:24 2006 From: a_marcuzzi <@t> cantv.net (Augusto Marcuzzi) Date: Tue May 30 12:32:28 2006 Subject: [Histonet] Slow draining paraffin dispenser Message-ID: <447C81A8.00001C.05552@PRINCIPAL> No virus found in this outgoing message. Mensaje saliente libre de virus Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.7.4/351 - Release Date: 29/05/2006 From ploykasek <@t> phenopath.com Tue May 30 12:54:49 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Tue May 30 12:55:03 2006 Subject: [Histonet] Ventana Benchmark closed systems vs Open system st ainers - a diatribe In-Reply-To: <0556BE8AC5551E4E8AF6BB9E42509BA20328D95C@usca0082k08.labvision.apogent.com> Message-ID: I think everyone has good comments and all points are valid. I expect the techs here to understand the IHC procedures, how & why they work. I want them to be able to trouble shoot no matter if the stain is manual or automated. They know the expected pattern of staining for the antibodies ? what is acceptable & what is not. This does require a great deal of training time, but it is time well spent. I can only state my experiences with the Dako Autostainer. I do like the "open" system. We make all of our rinse buffers and antigen retrieval buffers. I expect any registered tech working in the lab to be able to make & pH buffers. By having several different antigen retrieval buffers available, we can optimize each antibody. Plus, it saves money. I do think that a well trained tech can produce quality IHC stains off any instrument. Any instrument does require training & knowledge to fully utilize it. I find the Dako Autostainers user friendly, especially if you use the autoprogram feature. It doesn't take us very long at all to set the stainers up - we're running 4-5 stainers at a time, twice a day. All that being said, there is a lot of new technology out there. We will be evaluating several different stainers through out the rest of this year. I'd be happy to report back to the group on our experience. I think some of the things to consider when looking at instrumentation are quality of stain & instrument, # of techs in the lab & their experience level, work flow in the lab, and price per slide. I?m anxious to see how some of the newer instruments will affect these issues in our lab. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From Shirley_PHUA <@t> hsa.gov.sg Tue May 30 13:11:53 2006 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Tue May 30 13:12:15 2006 Subject: [Histonet] Shirley is away : 31 May 2006 (Wednesday) Message-ID: I will be out of the office from 31/05/2006 to 31/05/2006. I am away for 1 day. I will return on 01/06/2006 (Thursday). Pathologists : I will process your requests when I return. Otherwise, if urgent, please contact Henry. From GDawson <@t> dynacaremilwaukee.com Tue May 30 13:28:51 2006 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Tue May 30 13:29:06 2006 Subject: [Histonet] Ventana Benchmark closed systems vs Open system stainers - a diatribe Message-ID: Patti, Here, here...I agree wholeheartedly. Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Patti Loykasek Sent: Tuesday, May 30, 2006 11:55 AM To: histonet Subject: Re: [Histonet] Ventana Benchmark closed systems vs Open system stainers - a diatribe I think everyone has good comments and all points are valid. I expect the techs here to understand the IHC procedures, how & why they work. I want them to be able to trouble shoot no matter if the stain is manual or automated. They know the expected pattern of staining for the antibodies ? what is acceptable & what is not. This does require a great deal of training time, but it is time well spent. I can only state my experiences with the Dako Autostainer. I do like the "open" system. We make all of our rinse buffers and antigen retrieval buffers. I expect any registered tech working in the lab to be able to make & pH buffers. By having several different antigen retrieval buffers available, we can optimize each antibody. Plus, it saves money. I do think that a well trained tech can produce quality IHC stains off any instrument. Any instrument does require training & knowledge to fully utilize it. I find the Dako Autostainers user friendly, especially if you use the autoprogram feature. It doesn't take us very long at all to set the stainers up - we're running 4-5 stainers at a time, twice a day. All that being said, there is a lot of new technology out there. We will be evaluating several different stainers through out the rest of this year. I'd be happy to report back to the group on our experience. I think some of the things to consider when looking at instrumentation are quality of stain & instrument, # of techs in the lab & their experience level, work flow in the lab, and price per slide. I?m anxious to see how some of the newer instruments will affect these issues in our lab. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From a_marcuzzi <@t> cantv.net Tue May 30 13:35:01 2006 From: a_marcuzzi <@t> cantv.net (Augusto Marcuzzi) Date: Tue May 30 13:35:05 2006 Subject: [Histonet] Slow draining paraffin dispenser Message-ID: <447C9055.000024.05552@PRINCIPAL> No virus found in this outgoing message. Mensaje saliente libre de virus Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.7.4/351 - Release Date: 29/05/2006 From TJJ <@t> Stowers-Institute.org Tue May 30 13:35:41 2006 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Tue May 30 13:36:05 2006 Subject: [Histonet] Re: Cre, eGFP, and B-Gal Antibodies Message-ID: Hi Rhonda, I wish you nothing but luck with these! Out of the three, I'd say you will probably have success with the eGFP - it has been used successfully quite a bit in publications. We use Novus Biologicals rabbit polyclonal eGFP (new purified antibody) and we really like the results we're getting so far. Regarding Cre and B-gal, we have had no luck with either of them. The cre antibodies have not worked at all (but I think should work well on cultured cells), and the B-gal has inconsistent staining that I can't mirror with x-gal staining. Between the histology facility and the researchers at our institute, I think we've tried practically every Cre and B-gal commercially available and we're not getting any usable results. Let me know if you get some off list replies, I'm all ears (eyes!). Best wishes, Teri Johnson Stowers Institute for Medical Research Kansas City, MO 64110 From settembr <@t> umdnj.edu Tue May 30 13:37:08 2006 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Tue May 30 13:37:56 2006 Subject: [Histonet] Hairy cell leukaemia- CD103 Message-ID: I have hairy cell leukaemia, clone DBA44 that I have worked up for formlin fixed paraffin embedded (FFPE) human tissue. My director wants CD103 for hairy cell leukaemia. I know that there are vendors who cell CD103 for OTHER applications. Has anyone gotten CD103 to work on FFPE human tissue? paraffin. Thank you Dana Settembre University Hospital-UMDNJ Newark, NJ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> u.arizona.edu Tue May 30 14:00:37 2006 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Tue May 30 14:00:47 2006 Subject: [Histonet]Hospital costs/new topic In-Reply-To: <3cb.2ec570b.31addaa9@aol.com> Message-ID: <4.3.2.7.2.20060530115225.00cee720@algranth.inbox.email.arizona.edu> I'm sure that most of us would be amazed to see an itemized list of hospital charges. I had Mohs surgery in Feb. That was done at the Mohs surgeon's office and the charges were pretty much as I expected. Then two days later I had reconstruction surgery done to repair the defect - 3 1/2 hours of outpatient surgery and the hospital bill was over $19,000. This was not including the anesthesiologist and plastic surgeon's charges. Kind of blew me away. Thankfully my insurance plan is wonderful and my responsibility was very minimal but the itemized statement was wild! Andi At 01:28 PM 5/30/2006 -0400, you wrote: >I had to write when I read the post on hospital charges. A few months agao, >my husband was having what I was pretty certain was appendix pain so we went >in to the ER. After they established that he did in fact have a very ripe >appendix that needed to come out, we were in the ER for over 8 hours. He was >admitted after he came out of recovery and was there for ~27 hours. I always >like to take a look at the itemized bill after you are released. Its rather >amusing. There were so many charges for the nurses coming in and changing >his >bandages and bedding and I did most of that myself. Sometimes I had to >call and >practically beg them to come and change the bedding do drainage etc. They >rarely came by except on scheduled rounds. We had to empty his drain >tube, etc. >If I had a spare IV bag laying around, I would have changed that too >since the >alarm wouldnt shut off (alarm silence button was broken). I asked if we >could have some items to give him a sponge bath so he would feel a little >more >clean, get the betadine and other stuff off. They were real quick to >inform me >that that was MY job not theirs. Times have changes. Long story short, >unruptured appendix , 1 day in the hospital all total ~30k. They said >that if it >had ruptured it would have been closer to 5-7 days and we dont even want to >think about that. I was so thankful to have insurance. We only ended up >paying a >few thousand dollars. > > >Jodi >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From RRA <@t> Stowers-Institute.org Tue May 30 14:22:20 2006 From: RRA <@t> Stowers-Institute.org (Allen, Rhonda) Date: Tue May 30 14:22:39 2006 Subject: [Histonet]Hospital costs/new topic Message-ID: If you have an PPO insurance, like I had with Blue Cross, you have to pay 10% (I was lucky) of your hospital bill. Having a same day surgery my bill was $8800. I had to pay 10%. Now I switched to the Blue Cross HMO. If I have to have same day surgery again my bill will only be $300. Just think if you were an inpatient for a week or more. With the PPO you would have to pay 10% of the whole thing! I think the $300 a day co-pay will be much cheaper. Rhonda Allen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Tuesday, May 30, 2006 2:01 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet]Hospital costs/new topic I'm sure that most of us would be amazed to see an itemized list of hospital charges. I had Mohs surgery in Feb. That was done at the Mohs surgeon's office and the charges were pretty much as I expected. Then two days later I had reconstruction surgery done to repair the defect - 3 1/2 hours of outpatient surgery and the hospital bill was over $19,000. This was not including the anesthesiologist and plastic surgeon's charges. Kind of blew me away. Thankfully my insurance plan is wonderful and my responsibility was very minimal but the itemized statement was wild! Andi At 01:28 PM 5/30/2006 -0400, you wrote: >I had to write when I read the post on hospital charges. A few months >agao, my husband was having what I was pretty certain was appendix pain >so we went in to the ER. After they established that he did in fact >have a very ripe appendix that needed to come out, we were in the ER >for over 8 hours. He was admitted after he came out of recovery and was >there for ~27 hours. I always like to take a look at the itemized bill >after you are released. Its rather amusing. There were so many charges >for the nurses coming in and changing his bandages and bedding and I >did most of that myself. Sometimes I had to call and >practically beg them to come and change the bedding do drainage etc. They >rarely came by except on scheduled rounds. We had to empty his drain >tube, etc. >If I had a spare IV bag laying around, I would have changed that too >since the >alarm wouldnt shut off (alarm silence button was broken). I asked if we >could have some items to give him a sponge bath so he would feel a little >more >clean, get the betadine and other stuff off. They were real quick to >inform me >that that was MY job not theirs. Times have changes. Long story short, >unruptured appendix , 1 day in the hospital all total ~30k. They said >that if it >had ruptured it would have been closer to 5-7 days and we dont even want to >think about that. I was so thankful to have insurance. We only ended up >paying a >few thousand dollars. > > >Jodi >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bamoe <@t> gundluth.org Tue May 30 14:26:46 2006 From: bamoe <@t> gundluth.org (bamoe@gundluth.org) Date: Tue May 30 14:26:52 2006 Subject: [Histonet] Hematoxylin & eosin storage in flammable storage cabinet Message-ID: Hello all - We are doing some remodeling in our laboratory and losing some storage space where we currently store our hematoxylin and eosin. However, we are gaining a new flammable storage cabinet. Does anyone know of any incompatibility issues with storing Richard Allan Hematoxylin 2, Lerner Eosin-Y (alcoholic), ethyl alcohols (95% and absolute), and xylene together in a flammable storage cabinet? Thank you for any thoughts. Barb Moe Gundersen Lutheran Medical Center La Crosse WI From pruegg <@t> ihctech.net Tue May 30 15:06:20 2006 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue May 30 15:06:29 2006 Subject: [Histonet] tissue processing Message-ID: <200605302006.k4UK6KNO020169@chip.viawest.net> Is there anything bad that can happen to rat brains that have been snap frozen in OCT if we thaw-fix them in zinc formalin and process into paraffin? We froze these because we were not sure the IHC would work on ffpe tissue but have since gotten all the markers we are interested in to work in ffpe tissue. We would prefer to have the frozen brains in paraffin but do not want to risk any sort of damage to antigen sites especially and/or morphology preservation. Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From shirley.jen <@t> roche.com Tue May 30 15:24:52 2006 From: shirley.jen <@t> roche.com (Jen, Shirley) Date: Tue May 30 15:25:09 2006 Subject: [Histonet] Re: Meiosis and Mitosis In-Reply-To: <200605301710.CCX72728@mailgate3.roche.com> Message-ID: <6A39BF27EAB27743887E0425BD51196B0499A8DF@rplmsem1.nala.roche.com> Hi Lina, My experience is that you will find a good cell meisis (spermatogenesis) in Bouin's fixed paraffin section of testes and you will find some mitosis cell division in formalin fixed intestinal sections. Good Luck. Shirley Jen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Tuesday, May 30, 2006 10:11 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 30, Issue 44 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. anti-Abeta antibodies (Dumont, Nancy) 2. RE: Methods for meiosis and mitosis (Rittman, Barry R) 3. RE: To Ventana or not to Ventana (Deltour, Douglas D. (HM2)) 4. Ventana Benchmark (Sanders, Julie, VHACIN) 5. Ventana Benchmark XT (Malam Jacqueline) 6. question (gayle brosnanwatters) 7. RE: whole villi (Jackie M O'Connor) 8. Re: Ventana Benchmark (Jackie M O'Connor) 9. Energy Beam (Rita Riddle) 10. RE: Energy Beam (Weems, Joyce) 11. RE: Ventant Benchmark (Sebree Linda A.) 12. Cre, eGFP, and B-Gal Antibodies (Rogers, Rhonda) 13. slow draining paraffin dispenser (Walters, Katherine S) 14. Re: [Histonet]Hospital costs/new topic (Geoff McAuliffe) 15. RE: slow draining paraffin dispenser (Favara, Cynthia (NIH/NIAID) [E]) 16. RE: slow draining paraffin dispenser (Bonner, Janet) 17. floaters (Webb, Dorothy L) 18. Stent paper and Histonet connection (black) 19. Re: methyl green counterstain (Osborn, Barbara) 20. RE: Ventana Benchmark closed systems vs Open system st ainers - a diatribe (Morken, Tim - Labvision) 21. Re: floaters (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Mon, 29 May 2006 16:23:13 -0400 From: "Dumont, Nancy" Subject: [Histonet] anti-Abeta antibodies To: Message-ID: <1FAA5B74210C9A45A37A46EF6A969761076446@svrneurochem9.neurochem.local> Content-Type: text/plain; charset="iso-8859-1" Hello, I am looking for information on antibody affinity against Abeta peptide, at either the N-terminal part (such as 6E10 monoclonal antibody) or C terminal (such as polyclonal antibodies specific for A?40 or A?42 from Chemicon). Does anybody have some data sources to mention to me? I also have a question about pan-Abeta antibody. We constantly have cellular labeling with the pan-Abeta antibody from BioSource (sequence 15 to 30) when we process brain sections of transgenic mice. Does someone know if this antibody also labels APP? In fact, it seems that the labeling is indeed nuclear. Controls without primary or secondary antibodies are totally blank. A reference cited by the company also says that preabsorption of the antibody with the peptide leaded to the absence of any labeling. Thanks in advance for any advice! Nancy Dumont Research technician Neurochem Inc. Montreal, Canada L'information contenue dans ce courriel est confidentielle et prot?g?e par le secret professionnel. Elle n'est destin?e qu'? l'usage du destinataire indiqu? ci-dessus. Il est strictement interdit de distribuer ce document ou l'information qu'il contient. Si vous avez re?u ce courriel par erreur, ou s'il ne vous est pas destin?, veuillez le mentionner imm?diatement ? l'exp?diteur et d?truire tous les exemplaires de ce courriel. Merci. / This email is intended only for the party to whom it is addressed, and may contain information which is privileged or confidential. Any other disclosure is strictly prohibited. If you have received this email in error, or are not a named recipient, please notify the sender immediately and destroy all copies of this email. Thank you. ------------------------------ Message: 2 Date: Mon, 29 May 2006 18:51:30 -0500 From: "Rittman, Barry R" Subject: RE: [Histonet] Methods for meiosis and mitosis To: "Lina Vieira" , "Histonet" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Lina One of the simplest for mitosis is to use onion root tips, they grow rapidly and can process them via easilty. For meiosis can use mouse testis. Chromosomes easily seen using either Feulgen's reaction or iron hematoxylin. Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Lina Vieira Sent: Mon 5/29/2006 11:17 AM To: Histonet Subject: [Histonet] Methods for meiosis and mitosis Hi, I need to make some slides wich provide a good image of mitosis and meiosis for education purposes and I have only week to do it! Have You any suggestions? Protocols? Bibliography? Tanks in advance, Lina Vieira University of Algarve Portugal _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Tue, 30 May 2006 06:19:32 -0400 From: "Deltour, Douglas D. (HM2)" Subject: RE: [Histonet] To Ventana or not to Ventana To: "'Scott A. Ely'" , histonet@lists.utsouthwestern.edu Cc: histonet-owner@lists.utsouthwestern.edu Message-ID: <3F500F8B416C554EBB21FF16642F72E959CE9F@marxchg03.mar.med.navy.mil> Content-Type: text/plain I use two of the Benchmark XT's and love them. You could use the other open systems and cut down on cost per slide. You have to take into consideration your staffing. You can train other techs easily on the Benchmark. It would be much easier to rotate staff or make up for those sick days with the XT. If you tried to train them on an open system which includes making up buffers and so on, then it may be more of a headache. Good luck. Douglas Deltour HT(ASCP) -----Original Message----- From: Scott A. Ely [mailto:sae2001@med.cornell.edu] Sent: Friday, May 26, 2006 12:59 PM To: histonet@lists.utsouthwestern.edu Cc: histonet-owner@lists.utsouthwestern.edu Subject: [Histonet] Ventant Benchmark We are considering buying some new equipment. I would like to know who uses the Ventana Benchmark immunostainer or other immunostainers and what you like and don't like about it. Scott Ely, MD MPH Section of Hematopathology Department of Pathology Weill Medical College of Cornell University New York Presbyterian Hospital 525 E. 68th Street New York, NY 10021 PH: 212-746-2442 Legal Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the original intended recipient(s) selected by Dr. Ely and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the recipient specified by Dr. Ely, please contact the sender by reply e-mail and destroy all copies of the original message. ----- Original Message ----- From: histonet-request@lists.utsouthwestern.edu Date: Friday, May 26, 2006 12:29 pm Subject: Histonet Digest, Vol 30, Issue 39 ------------------------------ Message: 4 Date: Tue, 30 May 2006 07:06:39 -0400 From: "Sanders, Julie, VHACIN" Subject: [Histonet] Ventana Benchmark To: Message-ID: <2D4ACE41DEFE93428F23D77988EFBCB50E5FEA@VHAV10MSGA2.v10.med.va.gov> Content-Type: text/plain; charset="iso-8859-1" I agree with Joe, the cost of running the Ventana is high. My chief is always trying to figure out a way for us to not do immunos in house just because of this. With that said, our Ventana sales rep has given us the best prices possible for the volume we run, which is not that high compared to large hospitals/labs. I wish there were some way to keep the cost down for running this particular machine (we have a Benchmark XT), but there isn't and costs can only go up, as they do every year. However, we like Ventana, the technology, tech support, and sales support. Our pathologists would walk if we ever stopped using it as it has provided them with the best, most reliable technology we have found on the market. "How come every one talks about the reliability and walk away technology, but no one ever talks about the price of running these machines? Shouldn't cost be a factor also? I've been in budget meetings all week. The only thing I heard was how expensive it is to run immunos." Julie Sanders, BA, HT(ASCP) Supervisor, Anatomic Pathology Cincinnati VAMC ------------------------------ Message: 5 Date: Tue, 30 May 2006 12:12:37 +0100 From: Malam Jacqueline Subject: [Histonet] Ventana Benchmark XT To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain We have used a Benchmark XT for two and a half years now and it's been great. Advantages - complete standardisation - a noticeable saving in time as the antigen retrieval is automatic - 3 primary antisera incubation temperatures (it is interesting to discover that some like it at room temp and some like it hot!) - the variable antigen retrievals, primary incubation times and temperatures give plenty of choices for optimisation - making antisera up in "bulk" in prep kit dispensers saves you having to make them up each time with some waste and, with some antisera working at higher dilutions, this can lead to savings - prompt servicing, excellent help over the phone if you have to do some minor repairs or problem solving - even if you are not mechanically minded - and their English puts me to shame! - prompt dispatch of consumable orders - good training course in Strasbourg Disadvantages - routine maintenance can be a bit involved; e.g. you have to dismantle some of it to do a vortex mix check each month and it needs a decontamination every 3 months - water quality must be good - you don't want water tanks that aren't cleaned out annually and you need a decent de-ioniser or you can get micro-organisms getting through into the XT and furring up its arteries. You then have to sterilise all containers (bulk and those on the XT) with hypochlorite bleach before making or refilling with fresh buffer - it doesn't like some antisera clones like the oestrogen receptor clone 1D5; then again, some clones that didn't work too well manually or on other systems work well on the XT - just be aware when you are optimising if it doesn't work very well - try another clone - it may have a rather quiet alarm. Nothing could be done about it so, as our XT is in another room, we use a baby monitor Hope this helps Jacqui Malam Lancaster Infirmary UK DISCLAIMER: This e-mail is confidential and privileged. If you are not the intended recipient please accept our apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform postmaster@rli.mbht.nhs.uk that this message has gone astray before deleting it. Comments or opinions expressed in this email are those of their respective contributors only. The views expressed do not represent the views of the Trust, its management or employees. University Hospitals of Morecambe Bay NHS Trust is not responsible and disclaims any and all liability for the content of comments written within.Thank you for your co-operation. ------------------------------ Message: 6 Date: Tue, 30 May 2006 07:32:07 -0400 From: "gayle brosnanwatters" Subject: [Histonet] question To: Message-ID: <8172F14C39FCAF4E99E266F6756A3683076F9653@msfexch01.srunet.sruad.edu> Content-Type: text/plain; charset="us-ascii" Dear Listers, I don't know if this is an appropriate question, so please forgive me if it is not. I have decided to retire from academia at the end of the school year. I am a psychology professor, but I have been doing research in a small way, and you folks have been an enormous help several times. About 6 years ago I ended up owning a brand new Leica sliding microtome - I believe the model is the SM2000R. (It was a fluke - a retiring colleague shipped his old freezing microtome to me, the shipper dropped it, it was insured for full value, so I got this for "free." I have the receipt). I know that it is identical to the one they sell today for about 10K, which is what I paid for it. I did not buy the extra freezing part, but used it with an attachment that let me use dry ice for frozen sectioning. Anyway, I would like to sell it. Can anyone tell me where I could go to do so? It was only used about 10 times, as I ended up using my old ultramicrotome for all the work I've done. Someone told me that I should expect to get no less than 5K, and because it is in such good condition, and it is identical to the new ones, maybe up to 7. Does anyone have any suggestions? Again, I hope this is an appropriate question. I'd appreciate any help you can give me. Gayle L. Brosnan-Watters, PhD Assistant Professor Dept of Psychology Treasurer, Faculty for Undergraduate Neuroscience 226 Vincent Science Hall Slippery Rock University Slippery Rock, PA 16057 gayle.brosnanwatters@sru.edu 724-738-2529 - office 724-738-4807 - fax ------------------------------ Message: 7 Date: Tue, 30 May 2006 06:36:22 -0500 From: "Jackie M O'Connor" Subject: RE: [Histonet] whole villi To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" I process mouse GI routinely looking for Caspase 3 in the villi - We cut short lengths of small intestine, open it, and place it (lumen up) on bilbous paper which has been dipped in fixative. The segments stay on the paper all the way through paraffin processing. To embed, I remove the segments off the paper, and easily embed multiple segments on edge in one block. The villi remain undamaged. Jackie O' "Rittman, Barry R" Sent by: histonet-bounces@lists.utsouthwestern.edu 05/26/2006 10:36 AM To cc Subject RE: [Histonet] whole villi Renee Do you need to embed and cut them as a circle? If not I would suggest that you slit along the length and then place the connective tissue (or muscle) side down on a piece of card. The pieces should stick to the card. Then fix, remove from card and process as normal. Once they are fixed they should remain flat. If you have really big pieces can pin these to a piece of cork and float tissue side down in the fixative - pin using stainless steel pins, plastic pins or hedgehog quills. You will always have trouble in getting sections with entire lengths of villi in the jejunum because of the long length of these villi in this region. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Till, Renee Sent: Friday, May 26, 2006 10:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] whole villi I sometimes work with rat and mouse gi tissues, and as you might expect one of the most time consuming things is getting the tissues embedded good. The colon has not been as much of a problem, but lately we are doing more with the jejunum and getting whole villi is enough to make me scream. It seems to me that one of my main problems is that sometime between the collection of the tissues (which is not done by me) and when I embed them, the tissues often curl up and it is hard to get a good straight piece to embed. We embed them on end? (so when cut it makes a little O). Any ideas on how to keep them straight during the processing? Or maybe if there is something else that I am not thinking of that could be a problem. Thanks. Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72002 Office (501)364-2785 Fax (501)364-3161 ======================================================================== ======================== Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ======================================================================== ======================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Tue, 30 May 2006 06:55:17 -0500 From: "Jackie M O'Connor" Subject: Re: [Histonet] Ventana Benchmark To: "Joe Nocito" Cc: histonet@lists.utsouthwestern.edu, histonet-owner@lists.utsouthwestern.edu, "'Scott A. Ely'" , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Anybody been a patient in a hospital recently? I was - and NO I'm not on Medicare - I have really good insurance from my husband's plan at Motorola. I was in the hospital for 4 days - my bill was over $20K - the negotiated payment was about 15% of the actual billed amount. I don't understand how hospitals are managing to stay open only recovering 10% of the actual cost of services. Seems our economy is driven by the insurance companies. "Joe Nocito" Sent by: histonet-bounces@lists.utsouthwestern.edu 05/27/2006 05:56 PM To , "'Scott A. Ely'" , cc histonet-owner@lists.utsouthwestern.edu Subject Re: [Histonet] Ventant Benchmark Patsy, we charge what Medicare pays. Many insurance companies pay less. This is what fries my cookies. These people want to make money, yet pays trough the nose for immunos. Penny wise, dollar foolish is what my father would say. Joe ----- Original Message ----- From: To: "'Joe Nocito'" ; "'Scott A. Ely'" ; Cc: Sent: Saturday, May 27, 2006 10:25 AM Subject: RE: [Histonet] Ventant Benchmark >I am with you Joe. > I have not ever been able to understand how the labs can justify spending > so > much to run IHC, I guess they just pass the cost on to the patient, I can > do > the same thing for about 1/100 of the cost of running an instrument > like > the > Ventana using an open system like the Dakoautostainer and making up a lot > of > my own reagents like the buffers. I work in research and don't have a > money > cow like the patient health care system to milk. No wonder health care > costs are so,years ago when I worked in clinical we used to do what ever > we > could to try and help save the patient expense, sure doesn't seem like > that > is the case anymore. I know that understaffing, quick turn around and > untrained personnel is a big part of the problem here but come on, do you > really want someone who only knows how to apply a bar code to be > running your diagnostic IHC's? Bring on the flaming...... > Patsy > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito > Sent: Saturday, May 27, 2006 7:29 AM > To: Scott A. Ely; histonet@lists.utsouthwestern.edu > Cc: histonet-owner@lists.utsouthwestern.edu > Subject: Re: [Histonet] Ventant Benchmark > > flaming time > How come every one talks about the reliability and walk away > technology, > but > > no one ever talks about the price of running these machines? Shouldn't > cost > be a factor also? > I've been in budget meetings all week. The only thing I heard was how > expensive it is to run immunos. Yeah, we have two XTs, what do you expect? > > The opinions stated here are that of the author only and does not express > the opinions of his employer, lawyers, siblings, significant others > and their lawyers. > > Oh, by the way, I told the same thing to my Ventana Rep on Friday. He > knows, > > so y'all don't need to call my CEO. Y'all know who you are. > > Let the flaming begin. > > Joe Nocito BS, HT(ASCP)QIHC > Histology Manager > Pathology Reference Lab > San Antonio, TX > ----- Original Message ----- > From: "Scott A. Ely" > To: > Cc: > Sent: Friday, May 26, 2006 11:58 AM > Subject: [Histonet] Ventant Benchmark > > >> We are considering buying some new equipment. I would like to know >> who uses the Ventana Benchmark immunostainer or other immunostainers >> and what you like and don't like about it. >> >> Scott Ely, MD MPH >> Section of Hematopathology >> Department of Pathology >> Weill Medical College of Cornell University >> New York Presbyterian Hospital >> 525 E. 68th Street >> New York, NY 10021 >> PH: 212-746-2442 >> >> Legal Confidentiality Notice: This e-mail message, including any >> attachments, is for the sole use of the original intended >> recipient(s) selected by Dr. Ely and may contain confidential and > >> privileged information. Any >> unauthorized review, use, disclosure or distribution is prohibited. >> If >> you > >> are not the recipient specified by Dr. >> Ely, please contact the sender by reply e-mail and destroy all copies of >> the original message. >> >> ----- Original Message ----- >> From: histonet-request@lists.utsouthwestern.edu >> Date: Friday, May 26, 2006 12:29 pm >> Subject: Histonet Digest, Vol 30, Issue 39 >> >> > > > ---------------------------------------------------------------------------- > ---- > > >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > ---------------------------------------------------------------------------- > ---- > > >> > > > ---------------------------------------------------------------------------- > ---- > > > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.394 / Virus Database: 268.7.1/348 - Release Date: > 5/25/2006 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.394 / Virus Database: 268.7.2/349 - Release Date: 5/26/2006 > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Tue, 30 May 2006 09:00:39 -0400 From: Rita Riddle Subject: [Histonet] Energy Beam To: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Can someone give me the number to Energy Beam Sciences Inc. . Thanks Rita Riddle HT(ASCP) Lead Histology Tech Lexington Medical Center West Columbia,SC 29169 803-791-2881 _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. ------------------------------ Message: 10 Date: Tue, 30 May 2006 09:05:17 -0400 From: "Weems, Joyce" Subject: RE: [Histonet] Energy Beam To: "Rita Riddle" , Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202736C77@sjhaexc02.sjha.org> Content-Type: text/plain; charset="utf-8" 800-992-9037 or 860-653-0411 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rita Riddle Sent: Tuesday, May 30, 2006 9:01 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Energy Beam Can someone give me the number to Energy Beam Sciences Inc. . Thanks Rita Riddle HT(ASCP) Lead Histology Tech Lexington Medical Center West Columbia,SC 29169 803-791-2881 _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. ------------------------------ Message: 11 Date: Tue, 30 May 2006 08:13:20 -0500 From: "Sebree Linda A." Subject: RE: [Histonet] Ventant Benchmark To: "Weems, Joyce" , "Joe Nocito" , "Malcolm McCallum" , "Scott A. Ely" , Cc: histonet-owner@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="us-ascii" We also titer a number of our antibodies, especially those infrequently ordered. These we don't put in Ventana dispensers but rather manually apply them as all the VMS instruments allow one to do. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce Sent: Sunday, May 28, 2006 12:35 PM To: Joe Nocito; Malcolm McCallum; Scott A. Ely; histonet@lists.utsouthwestern.edu Cc: histonet-owner@lists.utsouthwestern.edu Subject: RE: [Histonet] Ventant Benchmark That's what I was saying too. Even with all our cost cutting attempts, do enough immunos to support 3 reagent-leased DAKO instruments. They're walk away too = after you get them loaded anyway! - and the reagents are not nearly as expensive. We titer all the antibodies that are available - a savings over the prepared Abs. The company has been a great partner for our laboratory. j Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Joe Nocito Sent: Sat 5/27/2006 6:58 PM To: Malcolm McCallum; Scott A. Ely; histonet@lists.utsouthwestern.edu Cc: histonet-owner@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventant Benchmark I'm not saying do it manually. I'm saying that there are less expensive ways to perform immunos than using Ventana's high cost reagents. Joe ----- Original Message ----- From: "Malcolm McCallum" To: "Joe Nocito" ; "Scott A. Ely" ; Cc: Sent: Saturday, May 27, 2006 10:39 AM Subject: RE: [Histonet] Ventant Benchmark Undoubtedly the cost and maintenance of automation is cheaper than the cost of additional employees salaries and benefits accompanied by associated duplication of manual systems. Additionally, equipment is depreciable, whereas human resources are a cost. VISIT THE JOURNAL HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-223-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Joe Nocito Sent: Sat 5/27/2006 8:29 AM To: Scott A. Ely; histonet@lists.utsouthwestern.edu Cc: histonet-owner@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventant Benchmark flaming time How come every one talks about the reliability and walk away technology, but no one ever talks about the price of running these machines? Shouldn't cost be a factor also? I've been in budget meetings all week. The only thing I heard was how expensive it is to run immunos. Yeah, we have two XTs, what do you expect? The opinions stated here are that of the author only and does not express the opinions of his employer, lawyers, siblings, significant others and their lawyers. Oh, by the way, I told the same thing to my Ventana Rep on Friday. He knows, so y'all don't need to call my CEO. Y'all know who you are. Let the flaming begin. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Scott A. Ely" To: Cc: Sent: Friday, May 26, 2006 11:58 AM Subject: [Histonet] Ventant Benchmark > We are considering buying some new equipment. I would like to know who > uses the Ventana Benchmark > immunostainer or other immunostainers and what you like and don't like > about it. > > Scott Ely, MD MPH > Section of Hematopathology > Department of Pathology > Weill Medical College of Cornell University > New York Presbyterian Hospital > 525 E. 68th Street > New York, NY 10021 > PH: 212-746-2442 > > Legal Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the original intended recipient(s) > selected by Dr. Ely and may contain confidential and > privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If you > are not the recipient specified by Dr. > Ely, please contact the sender by reply e-mail and destroy all copies of > the original message. > > ----- Original Message ----- > From: histonet-request@lists.utsouthwestern.edu > Date: Friday, May 26, 2006 12:29 pm > Subject: Histonet Digest, Vol 30, Issue 39 > > ------------------------------------------------------------------------ -------- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------------------------------------------------ -------- > ------------------------------------------------------------------------ -------- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.7.1/348 - Release Date: 5/25/2006 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.7.2/349 - Release Date: 5/26/2006 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. ------------------------------ Message: 12 Date: Tue, 30 May 2006 10:37:48 -0400 From: "Rogers, Rhonda" Subject: [Histonet] Cre, eGFP, and B-Gal Antibodies To: Message-ID: <6CB660A4F5C2D441961387F9CF60EBCA0C74A9@iu-mssg-mbx01.exchange.iu.edu> Content-Type: text/plain; charset="utf-8" Good morning, I will soon try Cre (from Novagen), eGFP (from Molecular Probes), and B-Gal (also from Molecular Probes) rabbit antibodies on formalin-fixed paraffin-embedded sections and/or frozen sections of mice embryos. Is anyone already using these antibodies for IHC staining and, if so, would you be willing to share your protocols? Thanks, Rhonda ------------------------------ Message: 13 Date: Tue, 30 May 2006 10:17:28 -0500 From: "Walters, Katherine S" Subject: [Histonet] slow draining paraffin dispenser To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi, I have Tissue Tek paraffin dispersing console Model 4586 (older model). It has worked well for many years, but lately it appears there is a partial clog, as it is dispensing much slower. Are there any (safe) protocols for clearing out these tubes? ------------------------------ Message: 14 Date: Tue, 30 May 2006 11:25:44 -0400 From: Geoff McAuliffe Subject: Re: [Histonet]Hospital costs/new topic To: Jackie M O'Connor Cc: Joe Nocito , histonet@lists.utsouthwestern.edu, histonet-owner@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu, "'Scott A. Ely'" Message-ID: <447C63F8.4030903@umdnj.edu> Content-Type: text/plain; format=flowed; charset=ISO-8859-1 Hospitals don't manage "to stay open only recovering 10% of the actual cost of services." That would be impossible. They overbill to cover the costs of indigent care, improvements, etc. The only people who pay full price are the uninsured who have no one to negotiate for them. Geoff Jackie M O'Connor wrote: >Anybody been a patient in a hospital recently? I was - and NO I'm not >on >Medicare - I have really good insurance from my husband's plan at >Motorola. >I was in the hospital for 4 days - my bill was over $20K - the negotiated >payment was about 15% of the actual billed amount. I don't understand >how hospitals are managing to stay open only recovering 10% of the actual >cost of services. Seems our economy is driven by the insurance >companies. > > > > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** ------------------------------ Message: 15 Date: Tue, 30 May 2006 11:42:32 -0400 From: "Favara, Cynthia \(NIH/NIAID\) [E]" Subject: RE: [Histonet] slow draining paraffin dispenser To: "Walters, Katherine S" , Message-ID: Content-Type: text/plain; charset="us-ascii" Hot hair dryer might help! c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives -----Original Message----- From: Walters, Katherine S [mailto:katherine-walters@uiowa.edu] Sent: Tuesday, May 30, 2006 8:17 AM To: histonet@pathology.swmed.edu Subject: [Histonet] slow draining paraffin dispenser Hi, I have Tissue Tek paraffin dispersing console Model 4586 (older model). It has worked well for many years, but lately it appears there is a partial clog, as it is dispensing much slower. Are there any (safe) protocols for clearing out these tubes? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Tue, 30 May 2006 11:56:33 -0400 From: "Bonner, Janet" Subject: RE: [Histonet] slow draining paraffin dispenser To: "Walters, Katherine S" , histonet@pathology.swmed.edu Message-ID: Content-Type: text/plain; charset=iso-8859-1 Try cleaning out the filter disk near the bottom, inside the tank. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Walters, Katherine S Sent: Tue 5/30/2006 11:17 AM To: histonet@pathology.swmed.edu Subject: [Histonet] slow draining paraffin dispenser Hi, I have Tissue Tek paraffin dispersing console Model 4586 (older model). It has worked well for many years, but lately it appears there is a partial clog, as it is dispensing much slower. Are there any (safe) protocols for clearing out these tubes? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Tue, 30 May 2006 11:04:17 -0500 From: "Webb, Dorothy L" Subject: [Histonet] floaters To: Histonet@lists.utsouthwestern.edu Message-ID: <0E394B648E5284478A6CCB78E5AFDA270171FDDB@hpes1.HealthPartners.int> Content-Type: text/plain; charset="US-ASCII" How does everyone handle their embedding techniques in regard to contaminating one tissue with another? We are doing here what I have done at the other lab I worked at and lately the pathologist is seeing a few "floaters" on a slide where they shouldn't be!! Just thought I would like to throw this out for discussion and hopefully come away with a technique better than ours!! Thanks, as always!!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. ------------------------------ Message: 18 Date: Thu, 25 May 2006 18:15:53 +0200 From: "black" Subject: [Histonet] Stent paper and Histonet connection To: Message-ID: <000701c680a3$a0c41f30$6725d0c4@yourdbba124d3a> Content-Type: text/plain; charset="iso-8859-1" To all Histonetters I just thought I should mention. that the stent paper would never have come about, if it had not been for the connections made through Histonet. This is such a valuable tool for sharing of knowledge and technical skills, and for net working with colleagues world wide, using similar technology. For this paper to have become a reality, the connection was made via histonet, which resulted in travel to Ottawa from South Africa and visa versa, as well as a poster in Savannah, Georgia. There are some extremely knowledgable people who regularly contribute to this network, to mention a few...Gayle Kallis, Chris V D Loos, John Kiernan etc.etc...and many more. WWe are very fortunate to have this technology at our disposal. Thanks to Histonet for keeping us all up to date!! Keep it up Melanie Black. ------------------------------ Message: 19 Date: Fri, 26 May 2006 16:05:37 -0400 From: "Osborn, Barbara" Subject: [Histonet] Re: methyl green counterstain To: ,"Histonet" Message-ID: <841D767DCDE87C49A02DA6DFC0BABABFA71F21@COMEXCHANGE.hscnet.hsc.usf.edu> Content-Type: text/plain; charset="US-ASCII" I believe it's the antigen retrieval that affects the methyl green counterstain. We purchase our methyl green from Vector Labs and although the staining is lighter than sections with no antigen retrieval, it does not fade out during dehydration. We counterstain for 10 min at 60oC with decent results. Barbara Osborn University of South Florida Message: 6 Date: Thu, 25 May 2006 17:50:03 -0400 From: "Jacqui Detmar" Subject: [Histonet] methyl green counterstain To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi all. I am doing some immunohistochemistry and TUNEL staining on mouse placental tissue. I have noticed that my methyl green counterstain on placentae assayed for TUNEL looks normal, but when I apply the same steps to tissue from the same block, but exposed to immunohistochemistry, the methyl green counterstain is very weak, bleaches out quickly during dehydration and generally looks pretty crappy. The methyl green recipe I am using is as follows: 0.5% methyl green in 0.1M sodium acetate buffer, pH 4.2. I would like to emphasize that no matter how quickly I dehydrate with the IHC sections, the colour is still very dim. The reason I am using methyl green is b/c I am doing IHC for nuclear proteins (Ki67, etc.) and don't want to use hematoxylin, since it's a little dark. Any suggestions? Thanks, Jacqui Detmar ------------------------------ Message: 20 Date: Tue, 30 May 2006 12:33:40 -0400 From: "Morken, Tim - Labvision" Subject: RE: [Histonet] Ventana Benchmark closed systems vs Open system st ainers - a diatribe To: histonet@lists.utsouthwestern.edu Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA20328D95C@usca0082k08.labvision.apogent.com> Content-Type: text/plain Wow Brian, Spring must have hit the Great White North! The sap is starting to run! Brian wrote (among other things): "As technologists we all want to see the perfect machine that allows us to load individual slides whenever we want, process them completely, error free and automatically right down to the coverslip, and do it for next to nothing. " Brian, you forgot the imaging system! The "Automated IHC System" also has to deliver the coverslipped slide to an imaging system that scans the entire tissue section to produce a Virtual Slide. (preferably at 40X and 10 focus levels, and then saves it (all 800 GB) to a server so anyone, anywhere (India?) can do the pathology consult (don't laugh, this is now happening in radiology)). We've actually heard this from customers. Lets, just say, yes, technically it can be done. But, few, if any, labs would have the half-million (US), or more, to buy the instrument. And you say you want it on reagent rental? You'd have to do thousands of slides a day to qualify. Maybe all the labs will eventually be consolidated into a few big labs and it will be possible. Not any time soon, though. Brian also wrote: "What I hope will happen, is that two or more companies will embrace the ongoing revenue stream model currently used by Ventana, and then we should see a rapid evolution towards truly automated IHC stainers. Competition is always brings out the best in industry. Look at how clinical chemistry analyzers now are able to process serum samples, with little need for the technologist to fuss with the process. That should be the goal of the IHC stainer manufacturer. " The dream of a totally automated IHC system for fixed tissue sections is not at all as simple as a chemistry or blood analysing system. In the clinical lab they are dealing with FRESH blood or fluid samples, all taken in standardized ways. The samples are nearly identical in every case. So a company is much more likely to be able to create instruments and reagents that work well over a broad range of labs. The big wrench in the IHC system is that no two labs have the same tissue procurement, fixation and processing system. Dr. Clive Taylor, the guru of standardization, has almost given up his crusade in convincing all labs to follow standardized procedures. For all their talk of wanting "the best" for their patients, no two pathologists can agree on what is "the best." Even the advent of "antigen retrieval" which was to overcome the fixation/processing variation problem, has itself become a complicated variable (with a slew of attendant instruments). So, considering infinite variation in tissue fixation and processing, AR and staining, it is nearly impossible to simply give a batch of reagents and an instrument to any given lab and have them all work perfectly from day one. In fact, every single antibody and instrument company in the business employs a cadre of Quality Control technologists who labor to be sure the antibodies sold are optimized and indeed "working," and they also employ a legion of "technical reps" who bravely go into unfamiliar labs and atttempt to "optimize" all the reagents, antibodies and protocols so that the system "works" in that lab. Believe me, there are very few labs that can do this successfully without some help. Besides that, do histotechs really want to be like Med Techs who just sit around looking at printouts all day long? Maybe not. We just like fiddling with things. Tim Morken Lab Vision - Neomarkers www.labvision.com Free webhosting for US State Histotechnology Societies: http://www.labvisioncorp.com/demowebsite/index.cfm -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brian Chelack Sent: Sunday, May 28, 2006 1:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana Benchmark closed systems vs Open system stainers - a diatribe Etc. Brian Chelack Prairie Diagnostic Services 2604-52 Campus Drive Saskatoon SK S7N 5B4 306-966-7241 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Tue, 30 May 2006 09:43:25 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] floaters To: "Webb, Dorothy L" , Histonet@lists.utsouthwestern.edu Message-ID: <20060530164325.8085.qmail@web61219.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Dorothy: First try to find the source of the floater. Compare the stained slide containing the floater with the block surface. If the tissue seen as floater is not in the block, it was picked up in the water bath. The solution is to clean the surface of the water with a tissue after each block is cut. If you can identify in the block the source of the floater it means that contamination occurred either during cassetting or during embedding. Make sure that the cassetting is done in a way that prevents carry-on of small pieces of tissue. The embedding center (the heating wells for the forceps) have to be empied with a pipette once embedding is finished. After that use a Q-tip to completely dry-clean the wells. Forceps should be cleaned after each block is embedded. Care is the solution and attention to detail is the only solution. Hope this will help you. Ren? J. "Webb, Dorothy L" wrote: How does everyone handle their embedding techniques in regard to contaminating one tissue with another? We are doing here what I have done at the other lab I worked at and lately the pathologist is seeing a few "floaters" on a slide where they shouldn't be!! Just thought I would like to throw this out for discussion and hopefully come away with a technique better than ours!! Thanks, as always!!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Feel free to call! Free PC-to-PC calls. Low rates on PC-to-Phone. Get Yahoo! Messenger with Voice ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 30, Issue 44 **************************************** From rjbuesa <@t> yahoo.com Tue May 30 15:29:21 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue May 30 15:29:25 2006 Subject: [Histonet] Hematoxylin & eosin storage in flammable storage cabinet In-Reply-To: Message-ID: <20060530202921.87037.qmail@web61225.mail.yahoo.com> Barb: I cannot think of any incompatibility. The alcoholic eosins are neither incompatible with pure ethanol nor with xylene. Hematoxylin is not flammable so it would be an "overkill" but as long as you can use the space I do not see why not. Hope this will help you. Ren? J. bamoe@gundluth.org wrote: Hello all - We are doing some remodeling in our laboratory and losing some storage space where we currently store our hematoxylin and eosin. However, we are gaining a new flammable storage cabinet. Does anyone know of any incompatibility issues with storing Richard Allan Hematoxylin 2, Lerner Eosin-Y (alcoholic), ethyl alcohols (95% and absolute), and xylene together in a flammable storage cabinet? Thank you for any thoughts. Barb Moe Gundersen Lutheran Medical Center La Crosse WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Sneak preview the all-new Yahoo.com. It's not radically different. Just radically better. From Kathy.Johnston <@t> CLS.ab.ca Tue May 30 15:43:11 2006 From: Kathy.Johnston <@t> CLS.ab.ca (Kathy.Johnston@CLS.ab.ca) Date: Tue May 30 15:43:25 2006 Subject: [Histonet]Hospital costs/new topic Message-ID: <4BC300747AF87A48BCDF8E48BC2885CE0136535E@mail1.calgary.com> Wow, glad I'm in Canada! Friend of mine had knee replacement surgery yesterday. Comes with A new knee, surgery, anaesthesia, 5-7 days stay in the hospital, and support afterwards. Cost for being able to walk again: $0. Might not be perfect up here, but being sick is your only worry. Kathy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Allen, Rhonda Sent: May 30, 2006 1:22 PM To: Andrea Grantham; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet]Hospital costs/new topic If you have an PPO insurance, like I had with Blue Cross, you have to pay 10% (I was lucky) of your hospital bill. Having a same day surgery my bill was $8800. I had to pay 10%. Now I switched to the Blue Cross HMO. If I have to have same day surgery again my bill will only be $300. Just think if you were an inpatient for a week or more. With the PPO you would have to pay 10% of the whole thing! I think the $300 a day co-pay will be much cheaper. Rhonda Allen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Tuesday, May 30, 2006 2:01 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet]Hospital costs/new topic I'm sure that most of us would be amazed to see an itemized list of hospital charges. I had Mohs surgery in Feb. That was done at the Mohs surgeon's office and the charges were pretty much as I expected. Then two days later I had reconstruction surgery done to repair the defect - 3 1/2 hours of outpatient surgery and the hospital bill was over $19,000. This was not including the anesthesiologist and plastic surgeon's charges. Kind of blew me away. Thankfully my insurance plan is wonderful and my responsibility was very minimal but the itemized statement was wild! Andi At 01:28 PM 5/30/2006 -0400, you wrote: >I had to write when I read the post on hospital charges. A few months >agao, my husband was having what I was pretty certain was appendix pain >so we went in to the ER. After they established that he did in fact >have a very ripe appendix that needed to come out, we were in the ER >for over 8 hours. He was admitted after he came out of recovery and was >there for ~27 hours. I always like to take a look at the itemized bill >after you are released. Its rather amusing. There were so many charges >for the nurses coming in and changing his bandages and bedding and I >did most of that myself. Sometimes I had to call and >practically beg them to come and change the bedding do drainage etc. They >rarely came by except on scheduled rounds. We had to empty his drain >tube, etc. >If I had a spare IV bag laying around, I would have changed that too >since the >alarm wouldnt shut off (alarm silence button was broken). I asked if we >could have some items to give him a sponge bath so he would feel a little >more >clean, get the betadine and other stuff off. They were real quick to >inform me >that that was MY job not theirs. Times have changes. Long story short, >unruptured appendix , 1 day in the hospital all total ~30k. They said >that if it >had ruptured it would have been closer to 5-7 days and we dont even want to >think about that. I was so thankful to have insurance. We only ended up >paying a >few thousand dollars. > > >Jodi >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. From Charles.Embrey <@t> carle.com Tue May 30 16:04:48 2006 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Tue May 30 16:04:55 2006 Subject: FW: [Histonet]Hospital costs/new topic Message-ID: -----Original Message----- From: Charles.Embrey Sent: Tuesday, May 30, 2006 4:03 PM To: 'Kathy.Johnston@CLS.ab.ca' Subject: RE: [Histonet]Hospital costs/new topic I only have one thing to say about Canadian Medicine "Tom Green". Charles Embrey, PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy.Johnston@CLS.ab.ca Sent: Tuesday, May 30, 2006 3:43 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet]Hospital costs/new topic Wow, glad I'm in Canada! Friend of mine had knee replacement surgery yesterday. Comes with A new knee, surgery, anaesthesia, 5-7 days stay in the hospital, and support afterwards. Cost for being able to walk again: $0. Might not be perfect up here, but being sick is your only worry. Kathy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Allen, Rhonda Sent: May 30, 2006 1:22 PM To: Andrea Grantham; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet]Hospital costs/new topic If you have an PPO insurance, like I had with Blue Cross, you have to pay 10% (I was lucky) of your hospital bill. Having a same day surgery my bill was $8800. I had to pay 10%. Now I switched to the Blue Cross HMO. If I have to have same day surgery again my bill will only be $300. Just think if you were an inpatient for a week or more. With the PPO you would have to pay 10% of the whole thing! I think the $300 a day co-pay will be much cheaper. Rhonda Allen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Tuesday, May 30, 2006 2:01 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet]Hospital costs/new topic I'm sure that most of us would be amazed to see an itemized list of hospital charges. I had Mohs surgery in Feb. That was done at the Mohs surgeon's office and the charges were pretty much as I expected. Then two days later I had reconstruction surgery done to repair the defect - 3 1/2 hours of outpatient surgery and the hospital bill was over $19,000. This was not including the anesthesiologist and plastic surgeon's charges. Kind of blew me away. Thankfully my insurance plan is wonderful and my responsibility was very minimal but the itemized statement was wild! Andi At 01:28 PM 5/30/2006 -0400, you wrote: >I had to write when I read the post on hospital charges. A few months >agao, my husband was having what I was pretty certain was appendix pain >so we went in to the ER. After they established that he did in fact >have a very ripe appendix that needed to come out, we were in the ER >for over 8 hours. He was admitted after he came out of recovery and was >there for ~27 hours. I always like to take a look at the itemized bill >after you are released. Its rather amusing. There were so many charges >for the nurses coming in and changing his bandages and bedding and I >did most of that myself. Sometimes I had to call and >practically beg them to come and change the bedding do drainage etc. They >rarely came by except on scheduled rounds. We had to empty his drain >tube, etc. >If I had a spare IV bag laying around, I would have changed that too >since the >alarm wouldnt shut off (alarm silence button was broken). I asked if we >could have some items to give him a sponge bath so he would feel a little >more >clean, get the betadine and other stuff off. They were real quick to >inform me >that that was MY job not theirs. Times have changes. Long story short, >unruptured appendix , 1 day in the hospital all total ~30k. They said >that if it >had ruptured it would have been closer to 5-7 days and we dont even want to >think about that. I was so thankful to have insurance. We only ended up >paying a >few thousand dollars. > > >Jodi >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Tue May 30 16:34:33 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue May 30 16:34:21 2006 Subject: [Histonet] Methods for meiosis and mitosis In-Reply-To: <004b01c6833b$73e340a0$2914100a@labhistologia> References: <004b01c6833b$73e340a0$2914100a@labhistologia> Message-ID: <447CBA69.1090209@umdnj.edu> The classic tissues used to demonstrate mitosis are onion root tip and whitefish blastula. You need tissue where there is a lot of cell division to see many stages, mitosis is a short lived event. This is why researchers interested in cell proloferation use things like radioactive thymidine incorporation or, these days, PCNA or bromodeoxyuridine labeling. The lable is a lot easier to 'catch' than a fleeting mititic figure. You could try the base of the crypts in the small intestine for mitosis if the others are not available. Or buy some slides from Carolina or Turtox or whatever biological supply house is in your country. Testis is good for meiosis but you will see many, many primary spermatocytes (meiosis I lasts a very long time) and very few secondary spermatocytes (meiosis II is very short). Good luck! Geoff Lina Vieira wrote: >Hi, >I need to make some slides wich provide a good image of mitosis and meiosis for education purposes and I have only week to do it! Have You any suggestions? Protocols? Bibliography? >Tanks in advance, > >Lina Vieira >University of Algarve >Portugal >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From Rcartun <@t> harthosp.org Tue May 30 18:26:44 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue May 30 18:27:17 2006 Subject: [Histonet] Surgery Center Message-ID: <447C9C740200007700000378@hcnwgwds01.hh.chs> Anyone (with Histology training) working in a Surgery Center (away from your hospital) doing frozen sections or tissue processing? If so, please contact me - I have a few questions. Thanks. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From laurie.reilly <@t> jcu.edu.au Tue May 30 18:33:40 2006 From: laurie.reilly <@t> jcu.edu.au (Laurie Reilly) Date: Tue May 30 18:32:53 2006 Subject: [Histonet] Re: Meiosis and Mitosis In-Reply-To: <6A39BF27EAB27743887E0425BD51196B0499A8DF@rplmsem1.nala.roc he.com> References: <200605301710.CCX72728@mailgate3.roche.com> Message-ID: <5.2.0.9.0.20060531091933.00bd8030@mail.jcu.edu.au> Dear All, Mitosis in mammalian cells is well demonstrated in the granulosa cell layer surrounding ovarian follicles. Pig ovary is good because they have many developing follicles at one time. These mitotic figures appear more like what is seen in neoplastic changes than do the mitotic figures seen in onion root tips, which always seem artificially clear. Regards, Laurie. At 01:24 PM 30/05/2006 -0700, Jen, Shirley wrote: >Hi Lina, >My experience is that you will find a good cell meisis (spermatogenesis) >in Bouin's fixed paraffin section of testes and you will find some mitosis >cell division in formalin fixed intestinal sections. >Good Luck. >Shirley Jen > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >histonet-request@lists.utsouthwestern.edu >Sent: Tuesday, May 30, 2006 10:11 AM >To: histonet@lists.utsouthwestern.edu >Subject: Histonet Digest, Vol 30, Issue 44 > > >Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > >To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > >You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > >When replying, please edit your Subject line so it is more specific than >"Re: Contents of Histonet digest..." > > >Today's Topics: > > 1. anti-Abeta antibodies (Dumont, Nancy) > 2. RE: Methods for meiosis and mitosis (Rittman, Barry R) > 3. RE: To Ventana or not to Ventana (Deltour, Douglas D. (HM2)) > 4. Ventana Benchmark (Sanders, Julie, VHACIN) > 5. Ventana Benchmark XT (Malam Jacqueline) > 6. question (gayle brosnanwatters) > 7. RE: whole villi (Jackie M O'Connor) > 8. Re: Ventana Benchmark (Jackie M O'Connor) > 9. Energy Beam (Rita Riddle) > 10. RE: Energy Beam (Weems, Joyce) > 11. RE: Ventant Benchmark (Sebree Linda A.) > 12. Cre, eGFP, and B-Gal Antibodies (Rogers, Rhonda) > 13. slow draining paraffin dispenser (Walters, Katherine S) > 14. Re: [Histonet]Hospital costs/new topic (Geoff McAuliffe) > 15. RE: slow draining paraffin dispenser > (Favara, Cynthia (NIH/NIAID) [E]) > 16. RE: slow draining paraffin dispenser (Bonner, Janet) > 17. floaters (Webb, Dorothy L) > 18. Stent paper and Histonet connection (black) > 19. Re: methyl green counterstain (Osborn, Barbara) > 20. RE: Ventana Benchmark closed systems vs Open system st ainers > - a diatribe (Morken, Tim - Labvision) > 21. Re: floaters (Rene J Buesa) > > >---------------------------------------------------------------------- > >Message: 1 >Date: Mon, 29 May 2006 16:23:13 -0400 >From: "Dumont, Nancy" >Subject: [Histonet] anti-Abeta antibodies >To: >Message-ID: > ><1FAA5B74210C9A45A37A46EF6A969761076446@svrneurochem9.neurochem.local> >Content-Type: text/plain; charset="iso-8859-1" > >Hello, > >I am looking for information on antibody affinity against Abeta peptide, >at either the N-terminal part (such as 6E10 monoclonal antibody) or C >terminal (such as polyclonal antibodies specific for A?40 or A?42 from >Chemicon). Does anybody have some data sources to mention to me? > >I also have a question about pan-Abeta antibody. We constantly have >cellular labeling with the pan-Abeta antibody from BioSource (sequence 15 >to 30) when we process brain sections of transgenic mice. Does someone >know if this antibody also labels APP? In fact, it seems that the >labeling is indeed nuclear. Controls without primary or secondary >antibodies are totally blank. A reference cited by the company also says >that preabsorption of the antibody with the peptide leaded to the absence >of any labeling. > >Thanks in advance for any advice! > > >Nancy Dumont >Research technician >Neurochem Inc. >Montreal, Canada >L'information contenue dans ce courriel est confidentielle et prot?g?e par >le secret professionnel. Elle n'est destin?e qu'? l'usage du destinataire >indiqu? ci-dessus. Il est strictement interdit de distribuer ce document >ou l'information qu'il contient. Si vous avez re?u ce courriel par erreur, >ou s'il ne vous est pas destin?, veuillez le mentionner imm?diatement ? >l'exp?diteur et d?truire tous les exemplaires de ce courriel. Merci. / > >This email is intended only for the party to whom it is addressed, and may >contain information which is privileged or confidential. Any other >disclosure is strictly prohibited. If you have received this email in >error, or are not a named recipient, please notify the sender immediately >and destroy all copies of this email. Thank you. > > >------------------------------ > >Message: 2 >Date: Mon, 29 May 2006 18:51:30 -0500 >From: "Rittman, Barry R" >Subject: RE: [Histonet] Methods for meiosis and mitosis >To: "Lina Vieira" , "Histonet" > >Message-ID: > >Content-Type: text/plain; charset="iso-8859-1" > >Lina >One of the simplest for mitosis is to use onion root tips, they grow >rapidly and can process them via easilty. For meiosis can use mouse >testis. Chromosomes easily seen using either Feulgen's reaction or iron >hematoxylin. Barry > >________________________________ > >From: histonet-bounces@lists.utsouthwestern.edu on behalf of Lina Vieira >Sent: Mon 5/29/2006 11:17 AM >To: Histonet >Subject: [Histonet] Methods for meiosis and mitosis > > > >Hi, >I need to make some slides wich provide a good image of mitosis and >meiosis for education purposes and I have only week to do it! Have You any >suggestions? Protocols? Bibliography? Tanks in advance, > >Lina Vieira >University of Algarve >Portugal >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >------------------------------ > >Message: 3 >Date: Tue, 30 May 2006 06:19:32 -0400 >From: "Deltour, Douglas D. (HM2)" >Subject: RE: [Histonet] To Ventana or not to Ventana >To: "'Scott A. Ely'" , > histonet@lists.utsouthwestern.edu >Cc: histonet-owner@lists.utsouthwestern.edu >Message-ID: > <3F500F8B416C554EBB21FF16642F72E959CE9F@marxchg03.mar.med.navy.mil> >Content-Type: text/plain > >I use two of the Benchmark XT's and love them. You could use the other >open systems and cut down on cost per slide. You have to take into >consideration your staffing. You can train other techs easily on the >Benchmark. It would be much easier to rotate staff or make up for those >sick days with the XT. If you tried to train them on an open system which >includes making up buffers and so on, then it may be more of a headache. >Good luck. > >Douglas Deltour HT(ASCP) > >-----Original Message----- >From: Scott A. Ely [mailto:sae2001@med.cornell.edu] >Sent: Friday, May 26, 2006 12:59 PM >To: histonet@lists.utsouthwestern.edu >Cc: histonet-owner@lists.utsouthwestern.edu >Subject: [Histonet] Ventant Benchmark > >We are considering buying some new equipment. I would like to know who >uses the Ventana Benchmark >immunostainer or other immunostainers and what you like and don't like >about it. > >Scott Ely, MD MPH >Section of Hematopathology >Department of Pathology >Weill Medical College of Cornell University >New York Presbyterian Hospital >525 E. 68th Street >New York, NY 10021 >PH: 212-746-2442 > >Legal Confidentiality Notice: This e-mail message, including any >attachments, is for the sole use of the original >intended recipient(s) selected by Dr. Ely and may contain confidential and >privileged information. Any >unauthorized review, use, disclosure or distribution is prohibited. If you >are not the recipient specified by Dr. >Ely, please contact the sender by reply e-mail and destroy all copies of >the original message. > >----- Original Message ----- >From: histonet-request@lists.utsouthwestern.edu >Date: Friday, May 26, 2006 12:29 pm >Subject: Histonet Digest, Vol 30, Issue 39 > > > >------------------------------ > >Message: 4 >Date: Tue, 30 May 2006 07:06:39 -0400 >From: "Sanders, Julie, VHACIN" >Subject: [Histonet] Ventana Benchmark >To: >Message-ID: > <2D4ACE41DEFE93428F23D77988EFBCB50E5FEA@VHAV10MSGA2.v10.med.va.gov> >Content-Type: text/plain; charset="iso-8859-1" > >I agree with Joe, the cost of running the Ventana is high. My chief is >always trying to figure out a way for us to not do immunos in house just >because of this. With that said, our Ventana sales rep has given us the >best prices possible for the volume we run, which is not that high >compared to large hospitals/labs. I wish there were some way to keep the >cost down for running this particular machine (we have a Benchmark XT), >but there isn't and costs can only go up, as they do every year. However, >we like Ventana, the technology, tech support, and sales support. Our >pathologists would walk if we ever stopped using it as it has provided >them with the best, most reliable technology we have found on the market. > >"How come every one talks about the reliability and walk away technology, but >no one ever talks about the price of running these machines? Shouldn't cost >be a factor also? >I've been in budget meetings all week. The only thing I heard was how >expensive it is to run immunos." > >Julie Sanders, BA, HT(ASCP) >Supervisor, Anatomic Pathology >Cincinnati VAMC > > > >------------------------------ > >Message: 5 >Date: Tue, 30 May 2006 12:12:37 +0100 >From: Malam Jacqueline >Subject: [Histonet] Ventana Benchmark XT >To: histonet@lists.utsouthwestern.edu >Message-ID: >Content-Type: text/plain > >We have used a Benchmark XT for two and a half years now and it's been great. >Advantages >- complete standardisation >- a noticeable saving in time as the antigen retrieval is automatic >- 3 primary antisera incubation temperatures (it is interesting to >discover that some like it at room temp and some like it hot!) >- the variable antigen retrievals, primary incubation times and >temperatures give plenty of choices for optimisation >- making antisera up in "bulk" in prep kit dispensers saves you having to >make them up each time with some waste and, with some antisera working at >higher dilutions, this can lead to savings >- prompt servicing, excellent help over the phone if you have to do some >minor repairs or problem solving - even if you are not mechanically minded >- and their English puts me to shame! >- prompt dispatch of consumable orders >- good training course in Strasbourg >Disadvantages >- routine maintenance can be a bit involved; e.g. you have to dismantle >some of it to do a vortex mix check each month and it needs a >decontamination every 3 months >- water quality must be good - you don't want water tanks that aren't >cleaned out annually and you need a decent de-ioniser or you can get >micro-organisms getting through into the XT and furring up its arteries. >You then have to sterilise all containers (bulk and those on the XT) with >hypochlorite bleach before making or refilling with fresh buffer >- it doesn't like some antisera clones like the oestrogen receptor clone >1D5; then again, some clones that didn't work too well manually or on >other systems work well on the XT - just be aware when you are optimising >if it doesn't work very well - try another clone >- it may have a rather quiet alarm. Nothing could be done about it so, as >our XT is in another room, we use a baby monitor Hope this helps > >Jacqui Malam >Lancaster Infirmary >UK > > > >DISCLAIMER: This e-mail is confidential and privileged. If you are not the >intended recipient please accept our apologies; please do not disclose, >copy or distribute information in this e-mail or take any action in >reliance on its contents: to do so is strictly prohibited and may be >unlawful. Please inform postmaster@rli.mbht.nhs.uk that this message has >gone astray before deleting it. Comments or opinions expressed in this >email are those of their respective contributors only. The views expressed >do not represent the views of the Trust, its management or employees. >University Hospitals of Morecambe Bay NHS Trust is not responsible and >disclaims any and all liability for the content of comments written >within.Thank you for your co-operation. > > > > >------------------------------ > >Message: 6 >Date: Tue, 30 May 2006 07:32:07 -0400 >From: "gayle brosnanwatters" >Subject: [Histonet] question >To: >Message-ID: > <8172F14C39FCAF4E99E266F6756A3683076F9653@msfexch01.srunet.sruad.edu> >Content-Type: text/plain; charset="us-ascii" > >Dear Listers, > > I don't know if this is an appropriate question, so please > forgive me if it is not. I have decided to retire from academia at the > end of the school year. I am a psychology professor, but I have been > doing research in a small way, and you folks have been an enormous help > several times. About 6 years ago I ended up owning a brand new Leica > sliding microtome - I believe the model is the SM2000R. (It was a fluke >- a retiring colleague shipped his old freezing microtome to me, the >shipper dropped it, it was insured for full value, so I got this for >"free." I have the receipt). I know that it is identical to the one they >sell today for about 10K, which is what I paid for it. I did not buy the >extra freezing part, but used it with an attachment that let me use dry >ice for frozen sectioning. Anyway, I would like to sell it. Can anyone >tell me where I could go to do so? It was only used about 10 times, as I >ended up using my old ultramicrotome for all the work I've done. Someone >told me that I should expect to get no less than 5K, and because it is in >such good condition, and it is identical to the new ones, maybe up to >7. Does anyone have any suggestions? > > Again, I hope this is an appropriate question. I'd >appreciate any help you can give me. > > > >Gayle L. Brosnan-Watters, PhD > >Assistant Professor > >Dept of Psychology > >Treasurer, Faculty for Undergraduate Neuroscience > >226 Vincent Science Hall > >Slippery Rock University > >Slippery Rock, PA 16057 > >gayle.brosnanwatters@sru.edu > >724-738-2529 - office > >724-738-4807 - fax > > > > > >------------------------------ > >Message: 7 >Date: Tue, 30 May 2006 06:36:22 -0500 >From: "Jackie M O'Connor" >Subject: RE: [Histonet] whole villi >To: histonet@lists.utsouthwestern.edu >Message-ID: > > >Content-Type: text/plain; charset="US-ASCII" > >I process mouse GI routinely looking for Caspase 3 in the villi - We cut >short lengths of small intestine, open it, and place it (lumen up) on >bilbous paper which has been dipped in fixative. The segments stay on the >paper all the way through paraffin processing. To embed, I remove the >segments off the paper, and easily embed multiple segments on edge in one >block. The villi remain undamaged. >Jackie O' > > > >"Rittman, Barry R" >Sent by: histonet-bounces@lists.utsouthwestern.edu >05/26/2006 10:36 AM > >To > >cc > >Subject >RE: [Histonet] whole villi > > > > > > >Renee >Do you need to embed and cut them as a circle? >If not I would suggest that you slit along the length and then place the >connective tissue (or muscle) side down on a piece of card. The pieces >should stick to the card. Then fix, remove from card and process as >normal. Once they are fixed they should remain flat. If you have really >big pieces can pin these to a piece of cork and float tissue side down in >the fixative - pin using stainless steel pins, plastic pins or hedgehog >quills. You will always have trouble in getting sections with entire >lengths of villi in the jejunum because of the long length of these villi >in this region. Barry > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Till, Renee >Sent: Friday, May 26, 2006 10:24 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] whole villi > >I sometimes work with rat and mouse gi tissues, and as you might expect >one of the most time consuming things is getting the tissues embedded >good. The colon has not been as much of a problem, but lately we are doing >more with the jejunum and getting whole villi is enough to make me scream. >It seems to me that one of my main problems is that sometime between the >collection of the tissues (which is not done by me) and when I embed them, >the tissues often curl up and it is hard to get a good straight piece to >embed. We embed them on end? (so when cut it makes a little O). Any ideas >on how to keep them straight during the processing? Or maybe if there is >something else that I am not thinking of that could be a problem. Thanks. > > > >Renee' Till, HT > >Research Assistant > >Arkansas Children's Nutrition Center > >1212 Marshall St./N2021 > >Little Rock, AR 72002 > >Office (501)364-2785 > >Fax (501)364-3161 > > > > >======================================================================== >======================== > >Confidentiality Notice: This e-mail message, including any attachments, is >for the sole use of the intended recipient(s) and may contain confidential >and privileged information. Any unauthorized review, use, disclosure or >distribution is prohibited. If you are not the intended recipient, please >contact the sender by reply e-mail and destroy all copies of the original >message. >======================================================================== >======================== _______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >------------------------------ > >Message: 8 >Date: Tue, 30 May 2006 06:55:17 -0500 >From: "Jackie M O'Connor" >Subject: Re: [Histonet] Ventana Benchmark >To: "Joe Nocito" >Cc: histonet@lists.utsouthwestern.edu, > histonet-owner@lists.utsouthwestern.edu, "'Scott A. Ely'" > , > histonet-bounces@lists.utsouthwestern.edu >Message-ID: > > >Content-Type: text/plain; charset="US-ASCII" > >Anybody been a patient in a hospital recently? I was - and NO I'm not on >Medicare - I have really good insurance from my husband's plan at >Motorola. >I was in the hospital for 4 days - my bill was over $20K - the negotiated >payment was about 15% of the actual billed amount. I don't understand >how hospitals are managing to stay open only recovering 10% of the actual >cost of services. Seems our economy is driven by the insurance >companies. > > > > > >"Joe Nocito" >Sent by: histonet-bounces@lists.utsouthwestern.edu >05/27/2006 05:56 PM > >To >, "'Scott A. Ely'" , > >cc >histonet-owner@lists.utsouthwestern.edu >Subject >Re: [Histonet] Ventant Benchmark > > > > > > >Patsy, >we charge what Medicare pays. Many insurance companies pay less. This is >what fries my cookies. These people want to make money, yet pays trough >the >nose for immunos. Penny wise, dollar foolish is what my father would say. > >Joe >----- Original Message ----- >From: >To: "'Joe Nocito'" ; "'Scott A. Ely'" >; >Cc: >Sent: Saturday, May 27, 2006 10:25 AM >Subject: RE: [Histonet] Ventant Benchmark > > > >I am with you Joe. > > I have not ever been able to understand how the labs can justify >spending > > so > > much to run IHC, I guess they just pass the cost on to the patient, I >can > > do > > the same thing for about 1/100 of the cost of running an instrument > > like > > > the > > Ventana using an open system like the Dakoautostainer and making up a >lot > > of > > my own reagents like the buffers. I work in research and don't have a > > money > > cow like the patient health care system to milk. No wonder health care > > costs are so,years ago when I worked in clinical we used to do what ever > > > we > > could to try and help save the patient expense, sure doesn't seem like > > that > > is the case anymore. I know that understaffing, quick turn around and > > untrained personnel is a big part of the problem here but come on, do >you > > really want someone who only knows how to apply a bar code to be > > running your diagnostic IHC's? Bring on the flaming...... > > Patsy > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe >Nocito > > Sent: Saturday, May 27, 2006 7:29 AM > > To: Scott A. Ely; histonet@lists.utsouthwestern.edu > > Cc: histonet-owner@lists.utsouthwestern.edu > > Subject: Re: [Histonet] Ventant Benchmark > > > > flaming time > > How come every one talks about the reliability and walk away > > technology, > > > but > > > > no one ever talks about the price of running these machines? Shouldn't > > cost > > be a factor also? > > I've been in budget meetings all week. The only thing I heard was how > > expensive it is to run immunos. Yeah, we have two XTs, what do you >expect? > > > > The opinions stated here are that of the author only and does not >express > > the opinions of his employer, lawyers, siblings, significant others > > and their lawyers. > > > > Oh, by the way, I told the same thing to my Ventana Rep on Friday. He > > knows, > > > > so y'all don't need to call my CEO. Y'all know who you are. > > > > Let the flaming begin. > > > > Joe Nocito BS, HT(ASCP)QIHC > > Histology Manager > > Pathology Reference Lab > > San Antonio, TX > > ----- Original Message ----- > > From: "Scott A. Ely" > > To: > > Cc: > > Sent: Friday, May 26, 2006 11:58 AM > > Subject: [Histonet] Ventant Benchmark > > > > > >> We are considering buying some new equipment. I would like to know > >> who uses the Ventana Benchmark immunostainer or other immunostainers > >> and what you like and don't like about it. > >> > >> Scott Ely, MD MPH > >> Section of Hematopathology > >> Department of Pathology > >> Weill Medical College of Cornell University > >> New York Presbyterian Hospital > >> 525 E. 68th Street > >> New York, NY 10021 > >> PH: 212-746-2442 > >> > >> Legal Confidentiality Notice: This e-mail message, including any > >> attachments, is for the sole use of the original intended > >> recipient(s) selected by Dr. Ely and may contain confidential and > > > >> privileged information. Any > >> unauthorized review, use, disclosure or distribution is prohibited. > >> If > >> you > > > >> are not the recipient specified by Dr. > >> Ely, please contact the sender by reply e-mail and destroy all copies >of > >> the original message. > >> > >> ----- Original Message ----- > >> From: histonet-request@lists.utsouthwestern.edu > >> Date: Friday, May 26, 2006 12:29 pm > >> Subject: Histonet Digest, Vol 30, Issue 39 > >> > >> > > > > > > >---------------------------------------------------------------------------- > > ---- > > > > > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > > > > > > >---------------------------------------------------------------------------- > > ---- > > > > > >> > > > > > > >---------------------------------------------------------------------------- > > ---- > > > > > > No virus found in this incoming message. > > Checked by AVG Free Edition. > > Version: 7.1.394 / Virus Database: 268.7.1/348 - Release Date: > > 5/25/2006 > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > > No virus found in this incoming message. > > Checked by AVG Free Edition. > > Version: 7.1.394 / Virus Database: 268.7.2/349 - Release Date: 5/26/2006 > > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >------------------------------ > >Message: 9 >Date: Tue, 30 May 2006 09:00:39 -0400 >From: Rita Riddle >Subject: [Histonet] Energy Beam >To: "'histonet@lists.utsouthwestern.edu'" > >Message-ID: > >Content-Type: text/plain; charset="iso-8859-1" > >Can someone give me the number to Energy Beam Sciences Inc. . > >Thanks > >Rita Riddle HT(ASCP) >Lead Histology Tech >Lexington Medical Center >West Columbia,SC 29169 >803-791-2881 > > >_________________________________ >This email and any files transmitted with it may contain PRIVILEGED or >CONFIDENTIAL information and may be read or used only by the intended >recipient. If you are not the intended recipient of the email or any of >its attachments, please be advised that you have received this email in >error and that any use, dissemination, distribution, forwarding, printing, >or copying of this email or any attached files is strictly prohibited. If >you have received this email in error, please immediately purge it and all >attachments and notify the sender by reply email or contact the sender at >the number listed above if one is provided. > > >------------------------------ > >Message: 10 >Date: Tue, 30 May 2006 09:05:17 -0400 >From: "Weems, Joyce" >Subject: RE: [Histonet] Energy Beam >To: "Rita Riddle" , > >Message-ID: > <1CD6831EB9B26D45B0A3EAA79F7EBD3202736C77@sjhaexc02.sjha.org> >Content-Type: text/plain; charset="utf-8" > >800-992-9037 or 860-653-0411 > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rita Riddle >Sent: Tuesday, May 30, 2006 9:01 AM >To: 'histonet@lists.utsouthwestern.edu' >Subject: [Histonet] Energy Beam > > >Can someone give me the number to Energy Beam Sciences Inc. . > >Thanks > >Rita Riddle HT(ASCP) >Lead Histology Tech >Lexington Medical Center >West Columbia,SC 29169 >803-791-2881 > > >_________________________________ >This email and any files transmitted with it may contain PRIVILEGED or >CONFIDENTIAL information and may be read or used only by the intended >recipient. If you are not the intended recipient of the email or any of >its attachments, please be advised that you have received this email in >error and that any use, dissemination, distribution, forwarding, printing, >or copying of this email or any attached files is strictly prohibited. If >you have received this email in error, please immediately purge it and all >attachments and notify the sender by reply email or contact the sender at >the number listed above if one is provided. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >Confidentiality Notice ** The information contained in this message may be >privileged and is confidential information intended for the use of the >addressee listed above. If you are neither the intended recipient nor the >employee or agent responsible for delivering this message to the intended >recipient, you are hereby notified that any disclosure, copying, >distribution or the taking of any action in reliance on the contents of >this information is strictly prohibited. If you have received this >communication in error, please notify us immediately by replying to the >message and deleting it from your computer. Thank you. Saint Joseph's >Health System, Inc. > >------------------------------ > >Message: 11 >Date: Tue, 30 May 2006 08:13:20 -0500 >From: "Sebree Linda A." >Subject: RE: [Histonet] Ventant Benchmark >To: "Weems, Joyce" , "Joe Nocito" > , "Malcolm McCallum" > , "Scott A. Ely" > , >Cc: histonet-owner@lists.utsouthwestern.edu >Message-ID: > >Content-Type: text/plain; charset="us-ascii" > >We also titer a number of our antibodies, especially those infrequently >ordered. These we don't put in Ventana dispensers but rather manually >apply them as all the VMS instruments allow one to do. > >Linda Sebree, HT(ASCP) >University of Wisconsin Hospital & Clinics >IHC/ISH Laboratory >A4/204-3224 >600 Highland Ave. >Madison, WI 53792 >(608)265-6596 >FAX: (608)262-7174 > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce >Sent: Sunday, May 28, 2006 12:35 PM >To: Joe Nocito; Malcolm McCallum; Scott A. Ely; >histonet@lists.utsouthwestern.edu >Cc: histonet-owner@lists.utsouthwestern.edu >Subject: RE: [Histonet] Ventant Benchmark > > >That's what I was saying too. Even with all our cost cutting attempts, do >enough immunos to support 3 reagent-leased DAKO instruments. They're walk >away too = after you get them loaded anyway! - and the reagents are not >nearly as expensive. We titer all the antibodies that are available >- a savings over the prepared Abs. The company has been a great partner >for our laboratory. j > >Joyce Weems >Pathology Manager >Saint Joseph's Hospital of Atlanta >404-851-7376 >404-851-7831 - Fax > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu on behalf of Joe Nocito >Sent: Sat 5/27/2006 6:58 PM >To: Malcolm McCallum; Scott A. Ely; histonet@lists.utsouthwestern.edu >Cc: histonet-owner@lists.utsouthwestern.edu >Subject: Re: [Histonet] Ventant Benchmark > >I'm not saying do it manually. I'm saying that there are less expensive ways >to perform immunos than using Ventana's high cost reagents. > >Joe >----- Original Message ----- >From: "Malcolm McCallum" >To: "Joe Nocito" ; "Scott A. Ely" >; >Cc: >Sent: Saturday, May 27, 2006 10:39 AM >Subject: RE: [Histonet] Ventant Benchmark > > >Undoubtedly the cost and maintenance of automation is cheaper than the cost >of additional employees salaries and benefits accompanied by associated >duplication of manual systems. Additionally, equipment is depreciable, >whereas human resources are a cost. > >VISIT THE JOURNAL HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org > > >Malcolm L. McCallum >Assistant Professor >Department of Biological Sciences >Texas A&M University Texarkana >2600 Robison Rd. >Texarkana, TX 75501 >O: 1-903-223-3134 >H: 1-903-791-3843 >Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html > > >________________________________ > >From: histonet-bounces@lists.utsouthwestern.edu on behalf of Joe Nocito >Sent: Sat 5/27/2006 8:29 AM >To: Scott A. Ely; histonet@lists.utsouthwestern.edu >Cc: histonet-owner@lists.utsouthwestern.edu >Subject: Re: [Histonet] Ventant Benchmark > > > >flaming time >How come every one talks about the reliability and walk away technology, >but no one ever talks about the price of running these machines? Shouldn't >cost be a factor also? I've been in budget meetings all week. The only >thing I heard was how expensive it is to run immunos. Yeah, we have two >XTs, what do you expect? > >The opinions stated here are that of the author only and does not express >the opinions of his employer, lawyers, siblings, significant others and >their lawyers. > >Oh, by the way, I told the same thing to my Ventana Rep on Friday. He >knows, so y'all don't need to call my CEO. Y'all know who you are. > >Let the flaming begin. > >Joe Nocito BS, HT(ASCP)QIHC >Histology Manager >Pathology Reference Lab >San Antonio, TX >----- Original Message ----- >From: "Scott A. Ely" >To: >Cc: >Sent: Friday, May 26, 2006 11:58 AM >Subject: [Histonet] Ventant Benchmark > > > > We are considering buying some new equipment. I would like to know >who > > uses the Ventana Benchmark > > immunostainer or other immunostainers and what you like and don't like > > about it. > > > > Scott Ely, MD MPH > > Section of Hematopathology > > Department of Pathology > > Weill Medical College of Cornell University > > New York Presbyterian Hospital > > 525 E. 68th Street > > New York, NY 10021 > > PH: 212-746-2442 > > > > Legal Confidentiality Notice: This e-mail message, including any > > attachments, is for the sole use of the original intended recipient(s) > > selected by Dr. Ely and may contain confidential >and > > privileged information. Any > > unauthorized review, use, disclosure or distribution is prohibited. If >you > > are not the recipient specified by Dr. > > Ely, please contact the sender by reply e-mail and destroy all copies >of > > the original message. > > > > ----- Original Message ----- > > From: histonet-request@lists.utsouthwestern.edu > > Date: Friday, May 26, 2006 12:29 pm > > Subject: Histonet Digest, Vol 30, Issue 39 > > > > > > >------------------------------------------------------------------------ >-------- > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > >------------------------------------------------------------------------ >-------- > > > > > > >------------------------------------------------------------------------ >-------- > > >No virus found in this incoming message. >Checked by AVG Free Edition. >Version: 7.1.394 / Virus Database: 268.7.1/348 - Release Date: 5/25/2006 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >-- >No virus found in this incoming message. >Checked by AVG Free Edition. >Version: 7.1.394 / Virus Database: 268.7.2/349 - Release Date: 5/26/2006 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > >Confidentiality Notice ** The information contained in this message may be >privileged and is confidential information intended for the use of the >addressee listed above. If you are neither the intended recipient nor the >employee or agent responsible for delivering this message to the intended >recipient, you are hereby notified that any disclosure, copying, >distribution or the taking of any action in reliance on the contents of >this information is strictly prohibited. If you have received this >communication in error, please notify us immediately by replying to the >message and deleting it from your computer. Thank you. Saint Joseph's >Health System, Inc. > > > >------------------------------ > >Message: 12 >Date: Tue, 30 May 2006 10:37:48 -0400 >From: "Rogers, Rhonda" >Subject: [Histonet] Cre, eGFP, and B-Gal Antibodies >To: >Message-ID: > ><6CB660A4F5C2D441961387F9CF60EBCA0C74A9@iu-mssg-mbx01.exchange.iu.edu> >Content-Type: text/plain; charset="utf-8" > >Good morning, > >I will soon try Cre (from Novagen), eGFP (from Molecular Probes), and >B-Gal (also from Molecular Probes) rabbit antibodies on formalin-fixed >paraffin-embedded sections and/or frozen sections of mice embryos. Is >anyone already using these antibodies for IHC staining and, if so, would >you be willing to share your protocols? > >Thanks, > >Rhonda > >------------------------------ > >Message: 13 >Date: Tue, 30 May 2006 10:17:28 -0500 >From: "Walters, Katherine S" >Subject: [Histonet] slow draining paraffin dispenser >To: >Message-ID: > > > >Content-Type: text/plain; charset="us-ascii" > >Hi, > >I have Tissue Tek paraffin dispersing console Model 4586 (older model). It >has worked well for many years, but lately it appears there is a partial >clog, as it is dispensing much slower. Are there any (safe) protocols for >clearing out these tubes? > > > > >------------------------------ > >Message: 14 >Date: Tue, 30 May 2006 11:25:44 -0400 >From: Geoff McAuliffe >Subject: Re: [Histonet]Hospital costs/new topic >To: Jackie M O'Connor >Cc: Joe Nocito , > histonet@lists.utsouthwestern.edu, > histonet-owner@lists.utsouthwestern.edu, > histonet-bounces@lists.utsouthwestern.edu, "'Scott A. Ely'" > >Message-ID: <447C63F8.4030903@umdnj.edu> >Content-Type: text/plain; format=flowed; charset=ISO-8859-1 > >Hospitals don't manage "to stay open only recovering 10% of the actual >cost of services." That would be impossible. >They overbill to cover the costs of indigent care, improvements, etc. >The only people who pay full price are the uninsured who have no one to >negotiate for them. > >Geoff > > >Jackie M O'Connor wrote: > > >Anybody been a patient in a hospital recently? I was - and NO I'm not > >on > >Medicare - I have really good insurance from my husband's plan at > >Motorola. > >I was in the hospital for 4 days - my bill was over $20K - the negotiated > >payment was about 15% of the actual billed amount. I don't understand > >how hospitals are managing to stay open only recovering 10% of the actual > >cost of services. Seems our economy is driven by the insurance > >companies. > > > > > > > > > > > > > > > > >-- >-- >********************************************** >Geoff McAuliffe, Ph.D. >Neuroscience and Cell Biology >Robert Wood Johnson Medical School >675 Hoes Lane, Piscataway, NJ 08854 >voice: (732)-235-4583; fax: -4029 >mcauliff@umdnj.edu >********************************************** > > > > > >------------------------------ > >Message: 15 >Date: Tue, 30 May 2006 11:42:32 -0400 >From: "Favara, Cynthia \(NIH/NIAID\) [E]" >Subject: RE: [Histonet] slow draining paraffin dispenser >To: "Walters, Katherine S" , > >Message-ID: > >Content-Type: text/plain; charset="us-ascii" > >Hot hair dryer might help! > >c > >Cynthia Favara >NIAID/NIH/RML/LPVD >903 South 4th Street >Hamilton, MT 59840 >406-363-9317 > >Disclaimer: >The information in this e-mail and any of its attachments is confidential >and may contain sensitive information. It should not be used by anyone who >is not the original intended recipient. If you have received this e-mail >in error please inform the sender and delete it from your mailbox or any >other storage devices. National Institute of Allergy and Infectious >Diseases shall not accept liability for any statements made that are >sender's own and not expressly made on behalf of the NIAID by one of its >representatives > >-----Original Message----- >From: Walters, Katherine S [mailto:katherine-walters@uiowa.edu] >Sent: Tuesday, May 30, 2006 8:17 AM >To: histonet@pathology.swmed.edu >Subject: [Histonet] slow draining paraffin dispenser > >Hi, > >I have Tissue Tek paraffin dispersing console Model 4586 (older model). It >has worked well for many years, but lately it appears there is a partial >clog, as it is dispensing much slower. Are there any (safe) protocols for >clearing out these tubes? > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >------------------------------ > >Message: 16 >Date: Tue, 30 May 2006 11:56:33 -0400 >From: "Bonner, Janet" >Subject: RE: [Histonet] slow draining paraffin dispenser >To: "Walters, Katherine S" , > histonet@pathology.swmed.edu >Message-ID: > > > >Content-Type: text/plain; charset=iso-8859-1 > >Try cleaning out the filter disk near the bottom, inside the tank. > >________________________________ > >From: histonet-bounces@lists.utsouthwestern.edu on behalf of Walters, >Katherine S >Sent: Tue 5/30/2006 11:17 AM >To: histonet@pathology.swmed.edu >Subject: [Histonet] slow draining paraffin dispenser > > > >Hi, > >I have Tissue Tek paraffin dispersing console Model 4586 (older model). >It has worked well for many years, but lately it appears there is a >partial clog, as it is dispensing much slower. Are there any (safe) >protocols for clearing out these tubes? > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >------------------------------ > >Message: 17 >Date: Tue, 30 May 2006 11:04:17 -0500 >From: "Webb, Dorothy L" >Subject: [Histonet] floaters >To: Histonet@lists.utsouthwestern.edu >Message-ID: > <0E394B648E5284478A6CCB78E5AFDA270171FDDB@hpes1.HealthPartners.int> >Content-Type: text/plain; charset="US-ASCII" > >How does everyone handle their embedding techniques in regard to >contaminating one tissue with another? We are doing here what I have done >at the other lab I worked at and lately the pathologist is seeing a few >"floaters" on a slide where they shouldn't be!! Just thought I would like >to throw this out for discussion and hopefully come away with a technique >better than ours!! Thanks, as always!!! >________________________________________ >This e-mail and any files transmitted with it are confidential and are >intended solely for the use of the individual or entity to whom they are >addressed. If you are not the intended recipient or the individual >responsible for delivering the e-mail to the intended recipient, please be >advised that you have received this e-mail in error and that any use, >dissemination, forwarding, printing, or copying of this e-mail is strictly >prohibited. > >If you have received this e-mail in error, please immediately notify the >HealthPartners Support Center by telephone at (952) 967-6600. You will be >reimbursed for reasonable costs incurred in notifying us. > > >------------------------------ > >Message: 18 >Date: Thu, 25 May 2006 18:15:53 +0200 >From: "black" >Subject: [Histonet] Stent paper and Histonet connection >To: >Message-ID: <000701c680a3$a0c41f30$6725d0c4@yourdbba124d3a> >Content-Type: text/plain; charset="iso-8859-1" > >To all Histonetters > >I just thought I should mention. that the stent paper would never have >come about, if it had not been for the connections made through Histonet. >This is such a valuable tool for sharing of knowledge and technical >skills, and for net working with colleagues world wide, using similar >technology. For this paper to have become a reality, the connection was >made via histonet, which resulted in travel to Ottawa from South Africa >and visa versa, as well as a poster in Savannah, Georgia. > >There are some extremely knowledgable people who regularly contribute to >this network, to mention a few...Gayle Kallis, Chris V D Loos, John >Kiernan etc.etc...and many more. WWe are very fortunate to have this >technology at our disposal. Thanks to Histonet for keeping us all up to date!! > >Keep it up >Melanie Black. > > >------------------------------ > >Message: 19 >Date: Fri, 26 May 2006 16:05:37 -0400 >From: "Osborn, Barbara" >Subject: [Histonet] Re: methyl green counterstain >To: ,"Histonet" > >Message-ID: > ><841D767DCDE87C49A02DA6DFC0BABABFA71F21@COMEXCHANGE.hscnet.hsc.usf.edu> > >Content-Type: text/plain; charset="US-ASCII" > >I believe it's the antigen retrieval that affects the methyl green >counterstain. We purchase our methyl green from Vector Labs and although >the staining is lighter than sections with no antigen retrieval, it does >not fade out during dehydration. We counterstain for 10 min at 60oC with >decent results. > > > >Barbara Osborn > >University of South Florida > > > >Message: 6 > >Date: Thu, 25 May 2006 17:50:03 -0400 > >From: "Jacqui Detmar" > >Subject: [Histonet] methyl green counterstain > >To: > >Message-ID: > > > >Content-Type: text/plain; charset="iso-8859-1" > > > >Hi all. I am doing some immunohistochemistry and TUNEL staining on mouse >placental tissue. I have noticed that my methyl green counterstain on >placentae assayed for TUNEL looks normal, but when I apply the same steps >to tissue from the same block, but exposed to immunohistochemistry, the >methyl green counterstain is very weak, bleaches out quickly during >dehydration and generally looks pretty crappy. The methyl green recipe I >am using is as follows: 0.5% methyl >green in 0.1M sodium acetate buffer, pH 4.2. I would like to >emphasize that no matter how quickly I dehydrate with the IHC sections, >the colour is still very dim. > > > >The reason I am using methyl green is b/c I am doing IHC for nuclear >proteins (Ki67, etc.) and don't want to use hematoxylin, since it's a >little dark. Any suggestions? > > > >Thanks, > > > >Jacqui Detmar > > > > > >------------------------------ > >Message: 20 >Date: Tue, 30 May 2006 12:33:40 -0400 >From: "Morken, Tim - Labvision" >Subject: RE: [Histonet] Ventana Benchmark closed systems vs Open > system st ainers - a diatribe >To: histonet@lists.utsouthwestern.edu >Message-ID: > ><0556BE8AC5551E4E8AF6BB9E42509BA20328D95C@usca0082k08.labvision.apogent.com> > >Content-Type: text/plain > >Wow Brian, Spring must have hit the Great White North! The sap is starting >to run! > >Brian wrote (among other things): > >"As technologists we all want to see the perfect machine that allows us to >load individual slides whenever we want, process them completely, error >free and automatically right down to the coverslip, and do it for next to >nothing. " > >Brian, you forgot the imaging system! The "Automated IHC System" also has >to deliver the coverslipped slide to an imaging system that scans the >entire tissue section to produce a Virtual Slide. (preferably at 40X and >10 focus levels, and then saves it (all 800 GB) to a server so anyone, anywhere >(India?) can do the pathology consult (don't laugh, this is now happening >in radiology)). > >We've actually heard this from customers. Lets, just say, yes, technically >it can be done. But, few, if any, labs would have the half-million (US), >or more, to buy the instrument. And you say you want it on reagent >rental? You'd have to do thousands of slides a day to qualify. Maybe all >the labs will eventually be consolidated into a few big labs and it will >be possible. Not any time soon, though. > >Brian also wrote: >"What I hope will happen, is that two or more companies will embrace the >ongoing revenue stream model currently used by Ventana, and then we should >see a rapid evolution towards truly automated IHC stainers. Competition is >always brings out the best in industry. Look at how clinical chemistry >analyzers now are able to process serum samples, with little need for the >technologist to fuss with the process. That should be the goal of the IHC >stainer manufacturer. " > >The dream of a totally automated IHC system for fixed tissue sections is >not at all as simple as a chemistry or blood analysing system. In the >clinical lab they are dealing with FRESH blood or fluid samples, all >taken in standardized ways. The samples are nearly identical in every >case. So a company is much more likely to be able to create instruments >and reagents that work well over a broad range of labs. > >The big wrench in the IHC system is that no two labs have the same tissue >procurement, fixation and processing system. Dr. Clive Taylor, the guru of >standardization, has almost given up his crusade in convincing all labs to >follow standardized procedures. For all their talk of wanting "the best" >for their patients, no two pathologists can agree on what is "the >best." Even the advent of "antigen retrieval" which was to overcome the >fixation/processing variation problem, has itself become a complicated >variable (with a slew of attendant instruments). > >So, considering infinite variation in tissue fixation and processing, AR >and staining, it is nearly impossible to simply give a batch of reagents >and an instrument to any given lab and have them all work perfectly from >day one. In fact, every single antibody and instrument company in the >business employs a cadre of Quality Control technologists who labor to be >sure the antibodies sold are optimized and indeed "working," and they >also employ a legion of "technical reps" who bravely go into unfamiliar >labs and atttempt to "optimize" all the reagents, antibodies and protocols >so that the system "works" in that lab. Believe me, there are very few >labs that can do this successfully without some help. > >Besides that, do histotechs really want to be like Med Techs who just sit >around looking at printouts all day long? Maybe not. We just like fiddling >with things. > > >Tim Morken >Lab Vision - Neomarkers >www.labvision.com > >Free webhosting for US State Histotechnology Societies: >http://www.labvisioncorp.com/demowebsite/index.cfm > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brian Chelack >Sent: Sunday, May 28, 2006 1:55 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Ventana Benchmark closed systems vs Open system stainers >- a diatribe > >Etc. > >Brian Chelack >Prairie Diagnostic Services >2604-52 Campus Drive >Saskatoon SK >S7N 5B4 >306-966-7241 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >------------------------------ > >Message: 21 >Date: Tue, 30 May 2006 09:43:25 -0700 (PDT) >From: Rene J Buesa >Subject: Re: [Histonet] floaters >To: "Webb, Dorothy L" , > Histonet@lists.utsouthwestern.edu >Message-ID: <20060530164325.8085.qmail@web61219.mail.yahoo.com> >Content-Type: text/plain; charset=iso-8859-1 > >Dorothy: > First try to find the source of the floater. > Compare the stained slide containing the floater with the block > surface. If the tissue seen as floater is not in the block, it was picked > up in the water bath. The solution is to clean the surface of the water > with a tissue after each block is cut. > > If you can identify in the block the source of the floater it means > that contamination occurred either during cassetting or during embedding. > Make sure that the cassetting is done in a way that prevents carry-on > of small pieces of tissue. The embedding center (the heating wells for > the forceps) have to be empied with a pipette once embedding is finished. > After that use a Q-tip to completely dry-clean the wells. Forceps should > be cleaned after each block is embedded. > Care is the solution and attention to detail is the only solution. > Hope this will help you. > Ren? J. > >"Webb, Dorothy L" wrote: > How does everyone handle their embedding techniques in regard to > contaminating one tissue with another? We are doing here what I have done > at the other lab I worked at and lately the pathologist is seeing a few > "floaters" on a slide where they shouldn't be!! Just thought I would like > to throw this out for discussion and hopefully come away with a technique > better than ours!! Thanks, as always!!! > ________________________________________ >This e-mail and any files transmitted with it are confidential and are >intended solely for the use of the individual or entity to whom they are >addressed. If you are not the intended recipient or the individual >responsible for delivering the e-mail to the intended recipient, please be >advised that you have received this e-mail in error and that any use, >dissemination, forwarding, printing, or copying of this e-mail is strictly >prohibited. > >If you have received this e-mail in error, please immediately notify the >HealthPartners Support Center by telephone at (952) 967-6600. You will be >reimbursed for reasonable costs incurred in notifying us. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Feel free to call! Free PC-to-PC calls. Low rates on PC-to-Phone. Get >Yahoo! Messenger with Voice > >------------------------------ > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >End of Histonet Digest, Vol 30, Issue 44 >**************************************** > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Mr.Laurie Reilly School of Veterinary and Biomedical Sciences James Cook University Townsville. 4811 Australia. Phone 07 4781 4468 Fax 07 4779 1526 From nfournier <@t> sasktel.net Tue May 30 18:06:08 2006 From: nfournier <@t> sasktel.net (Neil Fournier) Date: Tue May 30 18:36:13 2006 Subject: [Histonet] Rat Brain/Cryosectioning Message-ID: <001601c6843d$a38ce830$50de6e40@NEIL6FC9056E3D> Dear colleagues, Does any one have experience using liquid nitrogen cooled isopentane (2-methylbutane) on fixed (4% paraformaldehyde) rat brain tissue to quick freeze prior to sectioning (at approx 40 to 50 micron thick) on a cryostat? I would like to know if I am doing the procedure correctly. Our procedure is to block the brains and place them cryomolds that we then fill with OCT. We then place a small container of isopentane in the liquid nitrogen, and then submerge the entire cryomold block in cooled isopentane. I was told from colleague not to submerge the entire block in the solution, but I can't see any particular reason for not doing so. Lastly, how long should one typically keep the tissue submerged in the cooled isopentane? And how long should one keep the isopentane in the liquid nitrogen before using for freezing the biological tissue? Also does any one have strong reservations against using the liquid nitrogen cooled isopentane method for freezing rat brain tissue prior to sectioning? I have read that some use dry ice cooled isopentane; however, I have not seen any adequate explanation for one method over the other. Thanks for the help, Neil From abilger <@t> ptd.net Tue May 30 20:46:14 2006 From: abilger <@t> ptd.net (Andrea C. Bilger) Date: Tue May 30 20:46:59 2006 Subject: [Histonet] Ventana vs Dako Message-ID: <006301c68454$183a96d0$0300a8c0@andrea> I ave been reading with interest the mailings about the 2 top IP stainers. We have 2 Ventana XT's in our lab. Have found them very expensive to operate as everyone else has. The Pathologists wanted us to continue with Ventana due to only having one knowlegable IP tech and wanting consistancy. You have to have some knowlege of IP's even to operate the Ventana machines. Some reps make it sound goof proof but it isn't. We have had trouble with consistancy in a couple of detection kits. One was stronger and all our stains were too hot. Adjusted the stains and the next kit was back to the weaker strength. Had to adjust all the stains back again. What a pain in the ...... Has anyone had any experience with another IP stainer from Vision Biosystems? I looked at one at a National Histo convention and it sounded like a cross between the Dako and the Ventana. I think it was more of an open system but used bar codes on the slides. Antigen retrieval was done on the stainer. Reagents were supposed to be cheaper and not proprietary. It had a feature where you could start a stat stain after the run was started. I would be interested to hear anyones experience with this machine. Andrea From AnthonyH <@t> chw.edu.au Tue May 30 23:19:28 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue May 30 23:19:34 2006 Subject: [Histonet] Ventana vs Dako- BUT prefer the BOND Message-ID: Yes, We have a Bond Max and can't speak more highly of it. I have been doing IPXs since 1979, using the old Sternberger's PAP Complex method and later using ABC and then Polymer and unfortunately I had to concede that my manual results were never as good as what the Bond's can produce now. Results are consistent with no failed runs. It might be that Novacastra is now part of the Vision group of companies and their expertise and quality reagents have a lot to do with it. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea C. Bilger Sent: Wednesday, 31 May 2006 11:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana vs Dako I ave been reading with interest the mailings about the 2 top IP stainers. We have 2 Ventana XT's in our lab. Have found them very expensive to operate as everyone else has. The Pathologists wanted us to continue with Ventana due to only having one knowlegable IP tech and wanting consistancy. You have to have some knowlege of IP's even to operate the Ventana machines. Some reps make it sound goof proof but it isn't. We have had trouble with consistancy in a couple of detection kits. One was stronger and all our stains were too hot. Adjusted the stains and the next kit was back to the weaker strength. Had to adjust all the stains back again. What a pain in the ...... Has anyone had any experience with another IP stainer from Vision Biosystems? I looked at one at a National Histo convention and it sounded like a cross between the Dako and the Ventana. I think it was more of an open system but used bar codes on the slides. Antigen retrieval was done on the stainer. Reagents were supposed to be cheaper and not proprietary. It had a feature where you could start a stat stain after the run was started. I would be interested to hear anyones experience with this machine. Andrea _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From vanann702 <@t> skmc.gov.ae Wed May 31 00:03:10 2006 From: vanann702 <@t> skmc.gov.ae (Anne Van Binsbergen) Date: Wed May 31 00:01:01 2006 Subject: [Histonet] Acetic Acid Zinc Formalin Message-ID: Hi Histonetters I have in front of me a really good article from the AJ Clin Path about a trial comparing acetic acid zinc formalin to B5 fixative. Now - I have some REALLY difficult 'customers' here (aka pathologists) and I need some assistance please Did anyone of you out there participate in this study? TriCore reference labs, Albuquerque is named in the article Only MD's are mentioned Are there any other PUBLISHED articles that anyone knows of? I know that this is a contentious issue Hope to hear from you soon Regards Annie Anne S van Binsbegen Anatomical Pathology Laboratory Shaikh Khalifa Medical City PO BOX 51900 Abu Dhabu, U.A.E. ph: +971 2 6102695 mob:+971 50 3162804 fax +971 2 6104989 dance like no one is watching Sheikh Khalifa Medical City (SKMC) Disclaimer Notice: This mail message (including any attachments) is intended only for the designated recipient only and may contain privileged, proprietary, or otherwise private information. If you are not the addressee you must not copy, distribute, disclose or use any of the information in it. If you have received it in error, please notify the sender immediately and delete the original. Real women don't have hot flushes, they have power surges From Tony_Reilly <@t> health.qld.gov.au Wed May 31 01:56:24 2006 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Wed May 31 01:59:17 2006 Subject: [Histonet] Ventana vs Dako- BUT prefer the BOND Message-ID: I agree with everything Tony Henwood has said as my staining experience is very similar. I have only recently purchased the Bond Max. The main selling point was that it was much cheaper than the Ventana for reagents ( about 60%) even though it is a closed system instrument. It must be remembered that when you purchase their reagents you are paying for all of the time that has been spent by the manufacturer optimisig their system. This is often time not available in a busy clinical laboratory. Also it is an unfortunate fact that the bean counters that hold the purse strings would much rather a one off cost to purchase an automated system than commit to the cost of new staff even though the recurrent costs may outweigh the cost of an extra pair of hands. As a manager I have many responsibilities including running a quality, efficient and cost effective service. However I also have a responsibility to my staff and if I can get a walkawy instrument that will make their life easier rather than grind them into the ground because some beaurocrat in an office far removed from the coalface decides that their benchmarking barometer does not indicate that more staff will be coming down with next shower then I am more than happy to accept the costs involved. What I do not like particularly about the way these companies charge is that they will charge a different cost for reagents depending on the workload of a laboratory. We are lucky that we are a smaller lab wwhich is part of an organisation made up of 33 labs across the state, which means we can get a price based on the organisation workload rather than our own. If there are a lot of users in the US of the Ventana perhaps you could get a consortium going, use the power of numbers, and negotiate a better price. Not likely to happen, just a thought. regards Tony Reilly Chief Scientist Anatomical Pathology QHPS-Prince Charles Hospital Rode rd Chermside Q 4032 Australia Ph: 07 3350 8543 Fax: 07 33508546 tony_reilly@health.qld.gov.au >>> "Tony Henwood" 05/31/06 2:19 pm >>> Yes, We have a Bond Max and can't speak more highly of it. I have been doing IPXs since 1979, using the old Sternberger's PAP Complex method and later using ABC and then Polymer and unfortunately I had to concede that my manual results were never as good as what the Bond's can produce now. Results are consistent with no failed runs. It might be that Novacastra is now part of the Vision group of companies and their expertise and quality reagents have a lot to do with it. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea C. Bilger Sent: Wednesday, 31 May 2006 11:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana vs Dako I ave been reading with interest the mailings about the 2 top IP stainers. We have 2 Ventana XT's in our lab. Have found them very expensive to operate as everyone else has. The Pathologists wanted us to continue with Ventana due to only having one knowlegable IP tech and wanting consistancy. You have to have some knowlege of IP's even to operate the Ventana machines. Some reps make it sound goof proof but it isn't. We have had trouble with consistancy in a couple of detection kits. One was stronger and all our stains were too hot. Adjusted the stains and the next kit was back to the weaker strength. Had to adjust all the stains back again. What a pain in the ...... Has anyone had any experience with another IP stainer from Vision Biosystems? I looked at one at a National Histo convention and it sounded like a cross between the Dako and the Ventana. I think it was more of an open system but used bar codes on the slides. Antigen retrieval was done on the stainer. Reagents were supposed to be cheaper and not proprietary. It had a feature where you could start a stat stain after the run was started. I would be interested to hear anyones experience with this machine. Andrea _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/ received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. **************************************************************** From kemlo.rogerson <@t> waht.swest.nhs.uk Wed May 31 02:06:49 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed May 31 02:06:24 2006 Subject: [Histonet] Hematoxylin & eosin storage in flammable storage c abinet Message-ID: Haematoxylin is not flammable so does not need the flammable cupboard. Alcoholic Eosin is and so is xylene, but ought not the latter go in with the alcohols rather than with the stains? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 The greatest mistake in the treatment of diseases is that there are physicians for the body and physicians for the soul, although the two cannot be separated. --Plato This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From kemlo.rogerson <@t> waht.swest.nhs.uk Wed May 31 02:09:05 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed May 31 02:08:38 2006 Subject: [Histonet]Hospital costs/new topic Message-ID: We don't pay in the UK unless its dental, or you have a hip problem and don't want to wait for a year. Or yes if you are ugly you won't get that on the NHS unless plastic surgery is deemed necessary for psychological reasons. Personally I'd try it if I felt that my ugliness was affecting that way. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 The greatest mistake in the treatment of diseases is that there are physicians for the body and physicians for the soul, although the two cannot be separated. --Plato This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From kemlo.rogerson <@t> waht.swest.nhs.uk Wed May 31 02:11:15 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed May 31 02:11:18 2006 Subject: [Histonet] floaters Message-ID: We get floaters off the sea shore as Weston Super Mare, not sure what they are or were they 'leeked' from. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 The greatest mistake in the treatment of diseases is that there are physicians for the body and physicians for the soul, although the two cannot be separated. --Plato This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From DDDeltour <@t> mar.med.navy.mil Wed May 31 05:45:53 2006 From: DDDeltour <@t> mar.med.navy.mil (Deltour, Douglas D. (HM2)) Date: Wed May 31 05:46:12 2006 Subject: [Histonet] NOW Alcohol / Xylene storage in flammable storage cabinet Message-ID: <3F500F8B416C554EBB21FF16642F72E959CEA9@marxchg03.mar.med.navy.mil> This brings up a question! I have recently been wondering how many of you store Alcohol and Xylene in the same flam cabinet? I have been to multiple facilities which have multiple answers. If it is not "legal" then where can I find this in writing? Thanks Douglas Deltour HT(ASCP) -----Original Message----- From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Tuesday, May 30, 2006 4:29 PM To: bamoe@gundluth.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Hematoxylin & eosin storage in flammable storage cabinet Barb: I cannot think of any incompatibility. The alcoholic eosins are neither incompatible with pure ethanol nor with xylene. Hematoxylin is not flammable so it would be an "overkill" but as long as you can use the space I do not see why not. Hope this will help you. Ren? J. bamoe@gundluth.org wrote: Hello all - We are doing some remodeling in our laboratory and losing some storage space where we currently store our hematoxylin and eosin. However, we are gaining a new flammable storage cabinet. Does anyone know of any incompatibility issues with storing Richard Allan Hematoxylin 2, Lerner Eosin-Y (alcoholic), ethyl alcohols (95% and absolute), and xylene together in a flammable storage cabinet? Thank you for any thoughts. Barb Moe Gundersen Lutheran Medical Center La Crosse WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Sneak preview the all-new Yahoo.com. It's not radically different. Just radically better. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed May 31 07:11:35 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 31 07:11:39 2006 Subject: [Histonet] NOW Alcohol / Xylene storage in flammable storage cabinet In-Reply-To: <3F500F8B416C554EBB21FF16642F72E959CEA9@marxchg03.mar.med.navy.mil> Message-ID: <20060531121135.9879.qmail@web61216.mail.yahoo.com> Legal is one thing as safe is another. Some people fill their flammable cabinets to "the gills" not realizing that probably the most frightening scenario in a histology lab is a fire because I still have to see one with a sprinkler system. Instead of storing just the needed amounts for 1 or 2 days usually flammables cabinets are filled "up to capacity". There are outthere some regulations about having just 1 days supply, but there are no general standards on the subject. I always tried to have just 1 days supply and left the rest in the general store room of the hospital where they could handle any emergency better (they had sprinklers in the store room, we did not). Hope this will help you. Ren? J. "Deltour, Douglas D. (HM2)" wrote: This brings up a question! I have recently been wondering how many of you store Alcohol and Xylene in the same flam cabinet? I have been to multiple facilities which have multiple answers. If it is not "legal" then where can I find this in writing? Thanks Douglas Deltour HT(ASCP) -----Original Message----- From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Tuesday, May 30, 2006 4:29 PM To: bamoe@gundluth.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Hematoxylin & eosin storage in flammable storage cabinet Barb: I cannot think of any incompatibility. The alcoholic eosins are neither incompatible with pure ethanol nor with xylene. Hematoxylin is not flammable so it would be an "overkill" but as long as you can use the space I do not see why not. Hope this will help you. Ren? J. bamoe@gundluth.org wrote: Hello all - We are doing some remodeling in our laboratory and losing some storage space where we currently store our hematoxylin and eosin. However, we are gaining a new flammable storage cabinet. Does anyone know of any incompatibility issues with storing Richard Allan Hematoxylin 2, Lerner Eosin-Y (alcoholic), ethyl alcohols (95% and absolute), and xylene together in a flammable storage cabinet? Thank you for any thoughts. Barb Moe Gundersen Lutheran Medical Center La Crosse WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Sneak preview the all-new Yahoo.com. It's not radically different. Just radically better. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. From H.I.Grabsch <@t> leeds.ac.uk Wed May 31 09:32:27 2006 From: H.I.Grabsch <@t> leeds.ac.uk (Heike Grabsch) Date: Wed May 31 09:33:12 2006 Subject: [Histonet] antibodies against phosphorylated proteins Message-ID: I am working with formalin fixed paraffin embedded tissue and trying to detect phosphorylated proteins by immunohistochemistry. The antibodies are meant to be specific for the phosphorylated protein only. The one antibody I am trying to work up in particular is the phospho-H2AX from Upstate. Is there anybody out there who has an idea whether and if then how fixation may effect phosphorylation sides? Can they be lost or destroyed due to fixation? So the staining could be false negative? I hope somebody can comment on this Thanks, Heike Dr Heike Grabsch, MRCPath MD Gastrointestinal Research Group Pathology and Tumour Biology JIF Building, Level 4, Room 4.13 Leeds Institute for Molecular Medicine St James's University Hospital Beckett Street Leeds LS9 7TF United Kingdom phone 0044 113 3438626 fax 0044 113 3438431 From anh2006 <@t> med.cornell.edu Wed May 31 11:07:03 2006 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Wed May 31 11:06:20 2006 Subject: [Histonet] antibodies against phosphorylated proteins In-Reply-To: References: Message-ID: I have found as long as the tissue is fixed rapidly that you can easily detect phospho proteins in FFPE tissue. I have found that heat retrieval is key ... but I also have realized that providing you are using a reliable technique that the quality of staining is only as "good" as the antibody - as in all immunohistochemistry. I used to use a special fixative that contained sodium orthovanadate and some other ingredients to inhibit phosphatases but truthfully that caused more problems than it solved as the tissue was often not fixed well and embedding ended up being a nightmare. I recommend 10% NBF and just remember to process the tissues after removal from the organism as fast as possible. And as always remember to include controls :) Good luck! Andrea >I am working with formalin fixed paraffin embedded tissue and trying to >detect phosphorylated proteins by immunohistochemistry. The antibodies >are meant to be specific for the phosphorylated protein only. >The one antibody I am trying to work up in particular is the >phospho-H2AX from Upstate. >Is there anybody out there who has an idea whether and if then how >fixation may effect phosphorylation sides? Can they be lost or destroyed >due to fixation? So the staining could be false negative? >I hope somebody can comment on this > >Thanks, >Heike > >Dr Heike Grabsch, MRCPath MD >Gastrointestinal Research Group >Pathology and Tumour Biology >JIF Building, Level 4, Room 4.13 >Leeds Institute for Molecular Medicine >St James's University Hospital >Beckett Street >Leeds >LS9 7TF >United Kingdom > >phone 0044 113 3438626 >fax 0044 113 3438431 -- From petepath <@t> yahoo.com Wed May 31 11:07:35 2006 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Wed May 31 11:07:41 2006 Subject: [Histonet] Opening for pathologists assistant Message-ID: <20060531160735.6482.qmail@web30410.mail.mud.yahoo.com> We have an opening for a pathologists assistant at Hackensack University Medical Center in New Jersey. We handle around 33000 surgical specimens / year and share in the UMDNJ residency program. Competative salary and benifits. Great experience. If anyone knows someone who may be interested they can contact me at 201 996 4836 or petepath@yahoo.com. Stephen Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com From PMonfils <@t> Lifespan.org Wed May 31 11:15:03 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed May 31 11:15:13 2006 Subject: [Histonet] Extra hard PMMA? Message-ID: <09C945920A6B654199F7A58A1D7D1FDE0171771F@lsexch.lsmaster.lifespan.org> Does anyone have experience in using harder than usual PMMA for sectioning extremely hard specimens? What kind of softener, and how much, do you add to the methacrylate? Is it possible to section pure PMMA without any softeners added? (using a powerful motorized sliding microtome and tungsten carbide knives) From H.I.Grabsch <@t> leeds.ac.uk Wed May 31 11:25:30 2006 From: H.I.Grabsch <@t> leeds.ac.uk (Heike Grabsch) Date: Wed May 31 11:25:42 2006 Subject: [Histonet] antibodies against phosphorylated proteins Message-ID: Thanks Andrea. The problem is that I have no control at all about fixation of the tissues. I m aworking with human tissues from a pathology archive. Interestingly, you are talking about controls. Apart from negative controls - it is difficult to identifiy a positive one, as this will also be fixed tissue - or do you have a good suggestion for this? Heike > -----Original Message----- > From: Andrea T. Hooper [mailto:anh2006@med.cornell.edu] > Sent: 31 May 2006 17:07 > To: Heike Grabsch; Histonet > Subject: Re: [Histonet] antibodies against phosphorylated proteins > > I have found as long as the tissue is fixed rapidly that you can > easily detect phospho proteins in FFPE tissue. I have found that heat > retrieval is key ... but I also have realized that providing you are > using a reliable technique that the quality of staining is only as > "good" as the antibody - as in all immunohistochemistry. > > I used to use a special fixative that contained sodium orthovanadate > and some other ingredients to inhibit phosphatases but truthfully > that caused more problems than it solved as the tissue was often not > fixed well and embedding ended up being a nightmare. > > I recommend 10% NBF and just remember to process the tissues after > removal from the organism as fast as possible. And as always remember > to include controls :) > > Good luck! > Andrea > > > >I am working with formalin fixed paraffin embedded tissue and trying to > >detect phosphorylated proteins by immunohistochemistry. The antibodies > >are meant to be specific for the phosphorylated protein only. > >The one antibody I am trying to work up in particular is the > >phospho-H2AX from Upstate. > >Is there anybody out there who has an idea whether and if then how > >fixation may effect phosphorylation sides? Can they be lost or destroyed > >due to fixation? So the staining could be false negative? > >I hope somebody can comment on this > > > >Thanks, > >Heike > > > >Dr Heike Grabsch, MRCPath MD > >Gastrointestinal Research Group > >Pathology and Tumour Biology > >JIF Building, Level 4, Room 4.13 > >Leeds Institute for Molecular Medicine > >St James's University Hospital > >Beckett Street > >Leeds > >LS9 7TF > >United Kingdom > > > >phone 0044 113 3438626 > >fax 0044 113 3438431 > > -- From maa8 <@t> cornell.edu Wed May 31 12:24:02 2006 From: maa8 <@t> cornell.edu (Mary Ascenzi) Date: Wed May 31 12:24:10 2006 Subject: [Histonet] Ki67 formalin fixed liver In-Reply-To: Message-ID: <5.2.1.1.2.20060531131136.0962df90@postoffice6.mail.cornell.edu> Hello, I want to order Ki67 antibody to use with paraffin embedded formalin fixed woodchuck liver sections. Any advice on which companies to avoid or that give consistent results? Thanks, Mary Ascenzi Research Support Cornell University College of Vet Med Dept of Clinical Sciences From BSauer <@t> stjosephswb.com Wed May 31 12:54:48 2006 From: BSauer <@t> stjosephswb.com (Sauer, Barb) Date: Wed May 31 12:55:49 2006 Subject: [Histonet] EMPLOYMENT Message-ID: WE HAVE A H.T. OR H.T.L. POSITION AVAILABLE IMMEDIATELY. THIS IS A 90 BED FACILITY THAT SERVES THE COMMUNITY AND AREA CLINICS. THE HISTOLOGY DEPARTMENT DOES ROUTINE HISTOLOGY, BONE MARROWS, FROZEN SECTIONS, SPECIAL STAINS, LIMITED CYTOLOGY AND BASIC IMMUNO'S. IF YOU ARE A RECENT GRAD FROM A SCHOOL OF HISTOLOGY OR NEED A CHANGE PLEASE FEEL FREE TO CONTACT ME OR VIEW US ON THE WEBSITE www.stjosephswb.com . Barb Sauer H.T. Histology Department Synergy Health St. Joseph's Hospital West Bend, WI 53095 ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager. This footnote also confirms that this email message has been swept by MIMEsweeper for the presence of computer viruses. www.mimesweeper.com ********************************************************************** From jxccny <@t> yahoo.com Wed May 31 13:12:45 2006 From: jxccny <@t> yahoo.com (x j) Date: Wed May 31 13:12:50 2006 Subject: [Histonet] GAD vs. paraformaldehyde Message-ID: <20060531181246.97679.qmail@web51409.mail.yahoo.com> I read an article on http://www.histosearch.com/histonet/May00A/Re.GABBAandalcoholfix.html). It is very informative. I am doing GAD 65/67 experments. I have the following questions: 1). on the webpage http://www.histosearch.com/histonet/Aug02A/RE.4paraformaldehyde.html, it is said "It suggests that the solution was fresh and that very little formic acid or methanol had had time to form". If old paraformaldehyde solution is used, does the formic acid or methanol have negative effects on the later GAD staining? 2). Why people use alchol for perfusion and post fix ("profusing with 80% alcohol", "I drop mouse brains into fresh 80% (alcohol)... to post fix" in (http://www.histosearch.com/histonet/May00A/Re.GABBAandalcoholfix.html). It is very informative) Thank you very much! Jun __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From cfranci <@t> rigel.com Wed May 31 13:34:52 2006 From: cfranci <@t> rigel.com (Christian Franci) Date: Wed May 31 13:35:01 2006 Subject: [Histonet] eyeballs Message-ID: <974822ae42f54b701233aa88905464df@rigel.com> hello, you zany kids! does anybody out there have a good protocol for fixing/processing and paraffin embedding of mouse eyeballs? can/should it be done or is it better to go for frozen sections? Thanks in advance for any suggestions. cheers, Chris From sjchtascp <@t> yahoo.com Wed May 31 13:25:30 2006 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Wed May 31 13:52:15 2006 Subject: [Histonet] Primate Muscle Processing Message-ID: <20060531182530.36501.qmail@web38215.mail.mud.yahoo.com> I'm looking for a good processing protocol for mainly primate muscle. I have a copy of the NSH "Animal processing Manual" and use basicly the protocol #19 on page 11. The tissue is fixed at least 48 hrs in NBF proir to processing on a VIP. The sectioned tissue almost appears fragmented, not uniform. Would a different fixative be advisable?? Thanks, Steve --------------------------------- Ring'em or ping'em. Make PC-to-phone calls as low as 1?/min with Yahoo! Messenger with Voice. From gcallis <@t> montana.edu Wed May 31 14:30:27 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed May 31 14:30:40 2006 Subject: [Histonet] Primate Muscle Processing In-Reply-To: <20060531182530.36501.qmail@web38215.mail.mud.yahoo.com> References: <20060531182530.36501.qmail@web38215.mail.mud.yahoo.com> Message-ID: <6.0.0.22.1.20060531132217.01b60ec0@gemini.msu.montana.edu> Steven, Remember these are protocols that work for the people who wrote up the protocol and useful as guidelines. Formalin should work just fine, but that schedule also had temperature added to each processing station of alcohol and clearant - doing this only dries out tissues more, especially very lean animal tissues. There was no mention of paraffin either, and what type you use may make some diffference plus using 55C temperature is pretty low and your tissues are not infiltrated very well. 58 to 60C should work well also. Also the size of your sample, thickness may vary a bit also. You probably need to soak a trimmed block on ice water for a few minutes before sectioning to restore some moisture back to tissue block. At 12:25 PM 5/31/2006, you wrote: >I'm looking for a good processing protocol for mainly primate muscle. I >have a copy of the NSH "Animal processing Manual" and use basicly the >protocol #19 on page 11. > The tissue is fixed at least 48 hrs in NBF proir to processing on a > VIP. The sectioned tissue almost appears fragmented, not > uniform. Would a different fixative be advisable?? > > Thanks, > > Steve > > >--------------------------------- >Ring'em or ping'em. Make PC-to-phone calls as low as 1?/min with Yahoo! >Messenger with Voice. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From john <@t> greenhorn.org Wed May 31 14:31:25 2006 From: john <@t> greenhorn.org (john@greenhorn.org) Date: Wed May 31 14:31:37 2006 Subject: [Histonet] GAD vs. paraformaldehyde In-Reply-To: <20060531181246.97679.qmail@web51409.mail.yahoo.com> References: <20060531181246.97679.qmail@web51409.mail.yahoo.com> Message-ID: On 31 May 2006, at 19:12, x j wrote: > I read an article on > http://www.histosearch.com/histonet/May00A/ > Re.GABBAandalcoholfix.html). It is very informative. > > I am doing GAD 65/67 experments. I have the following questions: > > 1). on the webpage > http://www.histosearch.com/histonet/Aug02A/RE.4paraformaldehyde.html, > it is said "It suggests that the solution was fresh and that very > little formic acid or methanol had had time to form". If old > paraformaldehyde solution is used, does the formic acid or methanol > have negative effects on the later GAD staining? > > 2). Why people use alchol for perfusion and post fix ("profusing with > 80% alcohol", "I drop mouse > brains into fresh 80% (alcohol)... to post fix" in > (http://www.histosearch.com/histonet/May00A/ > Re.GABBAandalcoholfix.html). It is very informative) > > Thank you very much! > Jun > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Bograxie Inverurie. From sbreeden <@t> nmda.nmsu.edu Wed May 31 14:58:44 2006 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed May 31 14:58:48 2006 Subject: [Histonet] Issues with Tissues Message-ID: Actually, I think this would be a good motto for Histonet...."Issues with Tissues - resolved while you wait". But I digress. The Question of the Day is specifically for veterinary histology labs: "how long are you required to retain SLIDES?" My organization has concluded that we retain slides/blocks for ten years and I have issues with that length of time mainly for financial and space reasons. I'm gathering data for a full frontal attack on the status quo. And I'm certain I won't need External Flaming... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87108 From rjbuesa <@t> yahoo.com Wed May 31 15:14:37 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed May 31 15:14:42 2006 Subject: [Histonet] Ki67 formalin fixed liver In-Reply-To: <5.2.1.1.2.20060531131136.0962df90@postoffice6.mail.cornell.edu> Message-ID: <20060531201437.72245.qmail@web61220.mail.yahoo.com> I always bought monoclonal Ki-67 from Dako with consistent results. (HIER pH6) Ren? J. Mary Ascenzi wrote: Hello, I want to order Ki67 antibody to use with paraffin embedded formalin fixed woodchuck liver sections. Any advice on which companies to avoid or that give consistent results? Thanks, Mary Ascenzi Research Support Cornell University College of Vet Med Dept of Clinical Sciences _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail Beta. From cfavara <@t> niaid.nih.gov Wed May 31 16:57:39 2006 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID) [E]) Date: Wed May 31 16:57:44 2006 Subject: [Histonet] eyeballs In-Reply-To: <974822ae42f54b701233aa88905464df@rigel.com> Message-ID: I have been doing mouse eyes for a number of years. I have a Tissue Tek automated processor. If you are interested in the protocol I am happy to send it to you. If you look at the reference below you will see the quality if the work. c Am J Pathol. 2004 Dec;165(6):2055-67. Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives -----Original Message----- From: Christian Franci [mailto:cfranci@rigel.com] Sent: Wednesday, May 31, 2006 11:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] eyeballs hello, you zany kids! does anybody out there have a good protocol for fixing/processing and paraffin embedding of mouse eyeballs? can/should it be done or is it better to go for frozen sections? Thanks in advance for any suggestions. cheers, Chris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Wed May 31 18:20:17 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed May 31 18:20:24 2006 Subject: [Histonet] NOW Alcohol / Xylene storage in flammable storagecabinet Message-ID: I did not think that a water system would be appropriate for a xylene or alcohol fire. Wouldn't carbon dioxide or dry chemical be better? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, 31 May 2006 10:12 PM To: Deltour, Douglas D. (HM2); bamoe@gundluth.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NOW Alcohol / Xylene storage in flammable storagecabinet Legal is one thing as safe is another. Some people fill their flammable cabinets to "the gills" not realizing that probably the most frightening scenario in a histology lab is a fire because I still have to see one with a sprinkler system. Instead of storing just the needed amounts for 1 or 2 days usually flammables cabinets are filled "up to capacity". There are outthere some regulations about having just 1 days supply, but there are no general standards on the subject. I always tried to have just 1 days supply and left the rest in the general store room of the hospital where they could handle any emergency better (they had sprinklers in the store room, we did not). Hope this will help you. Ren? J. "Deltour, Douglas D. (HM2)" wrote: This brings up a question! I have recently been wondering how many of you store Alcohol and Xylene in the same flam cabinet? I have been to multiple facilities which have multiple answers. If it is not "legal" then where can I find this in writing? Thanks Douglas Deltour HT(ASCP) -----Original Message----- From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: Tuesday, May 30, 2006 4:29 PM To: bamoe@gundluth.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Hematoxylin & eosin storage in flammable storage cabinet Barb: I cannot think of any incompatibility. The alcoholic eosins are neither incompatible with pure ethanol nor with xylene. Hematoxylin is not flammable so it would be an "overkill" but as long as you can use the space I do not see why not. Hope this will help you. Ren? J. bamoe@gundluth.org wrote: Hello all - We are doing some remodeling in our laboratory and losing some storage space where we currently store our hematoxylin and eosin. However, we are gaining a new flammable storage cabinet. Does anyone know of any incompatibility issues with storing Richard Allan Hematoxylin 2, Lerner Eosin-Y (alcoholic), ethyl alcohols (95% and absolute), and xylene together in a flammable storage cabinet? Thank you for any thoughts. Barb Moe Gundersen Lutheran Medical Center La Crosse WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Sneak preview the all-new Yahoo.com. It's not radically different. Just radically better. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From lcastillos <@t> icoria.com Wed May 31 12:46:42 2006 From: lcastillos <@t> icoria.com (Castillos, Luminita) Date: Fri Jun 2 17:42:57 2006 Subject: [Histonet] NK markers for endometrial tissue Message-ID: Hello, Are there any NK markers for endometrial tissue that work using immunohistochemistry procedures on FFPE human tissues? Also, I would like to ask if somebody can share with me some H&E pictures of ectopic versus eutopic endometrium and what is the real definition of these two types of endometrium?. Thanks in advance, Luminita, Luminita Castillos, Ph.D. Research Scientist Cogenics(r), A Division of Clinical Data(r) Comprehensive Pharmacogenomics & Molecular Services(tm) 108 Alexader Dr. RTP, NC 27709 Tel: 919-425-2967 www.cogenics.com In 2005, Clinical Data, Inc. acquired Genaissance Pharmaceuticals, Lark Technologies, and Icoria, Inc., becoming the worldwide leader in the molecular diagnostic field. Cogenics, the pharmacogenomics and the molecular services division of Clinical Data specializes in the development and commercialization of molecular biology and pharmacogenomics solutions to biotechnology and pharmaceutical companies, academic, agricultural and government institutions. This communication and any file transmitted with it may contain information that is confidential, privileged and exempt from disclosure under applicable law. It is intended solely for the use of the individual or entity to which it is addressed. If you are not the intended recipient, you are hereby notified that any use, dissemination or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender. Thank you for your cooperation. From Yvonne.Thompson <@t> va.gov Tue May 30 14:56:20 2006 From: Yvonne.Thompson <@t> va.gov (Thompson, Yvonne) Date: Mon Jun 5 08:42:20 2006 Subject: [Histonet] AP supervisor position Message-ID: SEEKING AP SUPERVISOR Bay Pines VA Healthcare System located on the intercoastal waterway of the Gulf of Mexico, St. Petersburg, FL., is seeking a supervisor of Anatomic Pathology (AP) for a large, highly automated, multi-unit AP laboratory to include Autopsy, Cytology, Histology, Molecular Pathology and AP Office. A degree with a science emphasis is desired but not required. Proven supervisory experience/success is required. High level oral and writing skills are essential. HTL (ASCP) or equivalent required. FL license a plus but not required. TOD is M-F 8-4:30. Excellent benefits. Must be a US citizen. EOE. Contact Yvonne Thompson, 727-398-6661 X4596 for technical info. Mail Application packets to: Human Resources (05), Bay Pines VA Healthcare System, P.O. Box 5005, Bay Pines, FL 33744 or e mail annie.anderson@med.va.gov for application info. , From BSauer <@t> stjosephswb.com Tue May 30 15:01:02 2006 From: BSauer <@t> stjosephswb.com (Sauer, Barb) Date: Mon Jun 5 08:42:24 2006 Subject: [Histonet] employment Message-ID: To all H.T.'s and H.T.L.'s, We have a position open immediately at our facility. We are a small hospital that services surrounding clinics and offices. We do regular histology, frozens, special stains, bone marrows some cytology and immuno's. Please consider this if you need a change or are just finishing your training. It would be possible to log on to our website at www.stjosephs.org to learn more. Thanks, Barb Sauer H.T.(A.S.C.P.) Histology Lead Synergy Health/St. Joseph's Hospital 3200 Pleasant Valley Road West Bend, WI 53095 ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the system manager. This footnote also confirms that this email message has been swept by MIMEsweeper for the presence of computer viruses. www.mimesweeper.com **********************************************************************