From Lobke.DeBels <@t> UGent.be Wed Mar 1 05:30:29 2006 From: Lobke.DeBels <@t> UGent.be (Lobke De Bels) Date: Wed Mar 1 05:30:41 2006 Subject: [Histonet] collagen staining In-Reply-To: <20060228164912.16508.qmail@web33102.mail.mud.yahoo.com> References: <20060228164912.16508.qmail@web33102.mail.mud.yahoo.com> Message-ID: <1141212629.440585d54f9ea@mail.ugent.be> We also searched for a collagen staining with a good contrast for image analysis. This is the protocol we use: * biebrich scarlet-acid fushin solution (BS-AF) biebrich scarlet, aqueous 1% .... 90,0 ml acid fushin, aqueous 1%,......... 10,0 ml glacial acetic acid............... 1,0 ml * phoshomolybdic-phosphotungstic acid solution (PMA-PTA) phoshomolybdic acid ................5,0 g phosphotungstic acid ...............5,0 g distilled water...................200,0 ml * aniline blue solution (AB) aniline blue........................2,5 g glacial acetic acid.................2,0 ml distilled water....................100,0 ml * 1% glacial acetic acid solution glacial acetic acid.................1,0 ml distilled water....................100,0 ml staining procedure: - deparaffinize and hydrate to distilled water - BS/AF solution :3 X up and down - rinse in distilled water - PMA-PTA solution: 20 min - AB solution: 8 min - rinse in distilled water - glacial acetic solution: 8 min - dehydrate in alc 95%, isopropylalc, xyleen - mount DPX result: collagen => blue cytoplasm,keratin, muscle => red you can also use weigert's iron hematoxyline (10min) for the nuclei, but for the analysis it was better without for us. good luck Lobke De Bels Department of Morphology Faculty of Veterinary Medicine Ghent University Salisburylaan 133 9820 Merelbeke Belgium lobke.debels@ugent.be Citeren Galina Deyneko : > Dear Dr. Kiernan and Barbara, and colleagues, > I am also very interested in collagen staining using blue dyes for > better contrast for image analysis. For now we use picro-sirius red > overnight without counterstaining on mouse and rabbit hearts and arteries.. > Could you send me detailed protocol, > please.I mean: time of incubation, wash, differentiation ect. As well could > you give me a name of Dr. Kiernan book and where I can buy it. > Thank you at advance. > Galina Deyneko > Novartis Cambridge MA > 617-871-7613 > > > --------------------------------- > Yahoo! Mail > Bring photos to life! New PhotoMail makes sharing a breeze. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- From hts <@t> gator.net Wed Mar 1 07:04:36 2006 From: hts <@t> gator.net (hts@gator.net) Date: Wed Mar 1 07:04:40 2006 Subject: [Histonet] Histotech owned labs Message-ID: <3638.65.81.70.231.1141218276.squirrel@webmail.gru.net> How many out there own their own labs and have a Medical Director pathologist working with them to produce human diagnostic slides? I have just begun this process with a local pathologist and would greatly like some imput from some of my peers. Or how many out there have pathologist owned labs that are managed by histotechs and what percentage of the collectible insurance from Medicare technical are you receiving to keep the lab running. ie, what $ amount do you receive per specimen approximately? Thanks for your imput in advance. We want our pricing and experience with our pathologist to be fair and stay in business. Beverly From rschoon <@t> email.unc.edu Wed Mar 1 07:05:43 2006 From: rschoon <@t> email.unc.edu (Robert Schoonhoven) Date: Wed Mar 1 07:06:06 2006 Subject: [Histonet] Looking for PT evening/weekend work in RTP area Message-ID: <44059C27.2080405@email.unc.edu> HT/HTL (ASCP) with over 30 years exp. in all phases of histology looking for local part-time work. Heavy IHC and IA experiance over the last 15 years. Any one intrested please send me an e-mail and I'll send my Res. & CV. Thanks Robet Schoonhoven From Terry.Marshall <@t> rothgen.nhs.uk Wed Mar 1 07:09:34 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Wed Mar 1 07:09:36 2006 Subject: [Histonet] Revealing lymph nodes Message-ID: Sigh. This is an opportune discussion, because I have tried Davidson's in the past and could see no difference whatsoever between before and after. In the last few days we have made up a new batch, modified slightly to take into consideration JK's view that there should be a greater proportion of alcohol. Same result, no difference whatsoever. No clearing, no lymph nodes going white - nothing. Is this the Emperor's new cloths? PS I only tried it in the first place because of the regard I have for anything uttered by Bob Richmond. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: rsrichmond@aol.com [mailto:rsrichmond@aol.com] Sent: 28 February 2006 18:58 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Revealing lymph nodes I think that a clearing fixative (such as the examples already given; there are also commercial products) is essential for finding the small lymph nodes that are often positive for colon cancer in colon resection specimens. Remember that just one tiny positive lymph node upstages the tumor, and makes chemotherapy mandatory - so there's a real patient care decision involved here. In my travels as a locum tenens pathologist I noted that about half of pathologists were using clearing fixatives, and about half were not. As far as I know there have been no official pronouncements on the subject. Clearing fixatives are less necessary in axillary dissection material for breast cancer - the specimensare smaller and the lymph nodes - and metastases - are usually much bigger. The mesenteries from a colon cancer resection are voluminous and hard to search through. Bob Richmond Gastonia NC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pex0220 <@t> yahoo.com.cn Wed Mar 1 08:26:41 2006 From: pex0220 <@t> yahoo.com.cn (pex) Date: Wed Mar 1 08:26:50 2006 Subject: [Histonet] antibodies of bone marker Message-ID: <20060301142641.40939.qmail@web15509.mail.cnb.yahoo.com> Dear all, I would like to do immunostaing in mouse primary osteoblasts, so firstly I need some good antibodies of bone marker( such as alkaline phosphatase, osteocalcin, osteopontin, bone sialoprotein). At presnet some antibodies are there in our lab, however they do not work well. So I want to order some new antibodies. If you have much more experience about them in immunostaining, can you do me a favor? Tell me some information about antibodies( company, catalogue number, or lab, etc) Thanks a lot Guofeng Germany --------------------------------- Ïë³ÉΪ·ëС¸Õ¡¢³Â¿­¸è¡¢ÕżÍÖÐÈý´óµ¼ÑݵÄÖ÷½ÇÂð£¿From hej01 <@t> health.state.ny.us Wed Mar 1 08:49:44 2006 From: hej01 <@t> health.state.ny.us (Helen E Johnson) Date: Wed Mar 1 08:49:56 2006 Subject: [Histonet] animal pathology database Message-ID: Hi Histonetters, I need to have information in the developement of an animal pathology database. Your help would be appreciated. Thanks. Helen (hej01@health.state.ny.us) From TJJ <@t> Stowers-Institute.org Wed Mar 1 10:48:26 2006 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Wed Mar 1 10:48:56 2006 Subject: [Histonet] Spam - OT Message-ID: How funny, yesterday in my personal email list I received one with the subject line: Re: ovary. For a moment I considered opening it, then I realized it was my personal email and not from anybody I recognized. Most times I don't give it a second thought but delete these things quickly. Yet, something with this subject line might well catch histologists and pathologists unaware and actually get opened. What a strange, yet interesting choice of words. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64133 From kemlo.rogerson <@t> waht.swest.nhs.uk Wed Mar 1 10:53:43 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Mar 1 10:53:56 2006 Subject: [Histonet] Spam - OT Message-ID: I only started receiving spam when I joined Histonet, now I get daily offerings to enlarge things either by Surgery or medicinally, odd that! How do they know? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 "Following the line of least resistance makes both men and rivers crooked" This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Johnson, Teri [mailto:TJJ@Stowers-Institute.org] Sent: Wednesday, March 01, 2006 4:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Spam - OT How funny, yesterday in my personal email list I received one with the subject line: Re: ovary. For a moment I considered opening it, then I realized it was my personal email and not from anybody I recognized. Most times I don't give it a second thought but delete these things quickly. Yet, something with this subject line might well catch histologists and pathologists unaware and actually get opened. What a strange, yet interesting choice of words. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64133 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Vickroy.Jim <@t> mhsil.com Wed Mar 1 11:00:56 2006 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Wed Mar 1 11:03:16 2006 Subject: [Histonet] MICROWAVE Message-ID: Our current scientific microwave is on it's last leg. Any suggestions? Companies? We use it for antigen retrieval and some special stains. James R. Vickroy Supervisor - MMC Surgical Pathology 217-788-4046 This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From sbreeden <@t> nmda.nmsu.edu Wed Mar 1 11:35:49 2006 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Mar 1 11:35:53 2006 Subject: [Histonet] Need ++ BVD Control Message-ID: I am in need of good positive BVD (bovine viral diarrhea) control tissue. In exchange, I can offer Blastomycosis ++ blocks or AFB ++ blocks. I also have a few (5) ++ Cu control blocks. My tissues are, obviously, of animal origin. If you are willing to trade (or even "donate"!), please contact me. If you have extra "bails" (a.k.a. "handles") for the Sakura slide racks (the racks that are used with the auto-stainer and Tissue-Tek tape coverslipper) and you no longer have need of them, I could use them and would be willing to discuss a trade of some sort (New Mexico green chile???). Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services POBox 4700 Albuquerque, NM 87196 (505)841-2576 (505)841-2518 FAX From david.kinsley <@t> spcorp.com Wed Mar 1 12:32:04 2006 From: david.kinsley <@t> spcorp.com (Kinsley, David) Date: Wed Mar 1 12:44:29 2006 Subject: [Histonet] Help with mouse adipose tissue Message-ID: Hi everyone, I'm working on a project that involves collecting adipose tissue from multiple sites of a mouse for the purpose of IHC. I fixed my samples in 4% paraformaldehyde for 22 hours, rinsed in tap water to remove excess formalin, then put them into 70% ETOH for 10 days and then processed on my routine mouse program. (30 min each in 80%, 95%, 95%, 100%, 100%, 100% ETOH then 30 min. each in 3 changes of Xylene, then 1 hour each in 3 changes of paraffin.) I test sectioned 2 of the blocks to make sure they sectioned well. The first block was a smaller piece of fat from between the abdominal muscles and skin. It sectioned well and even stayed intact on a 50 degree water bath. The second piece (larger in size) from the epidydimal area was more difficult to cut, but I was able to float it on a room temp water bath and obtain sections good enough for H&E staining. When I sectioned both blocks again 2 days later, the smaller piece still cut well, but the larger piece did not cut. I tried ice with no water, and even freezing spray but both did not help. Does anyone have suggestions? If I need to reprocess what would be the best procedure to follow since these are for IHC? thanks in advance David Kinsley Schering-Plough Research Institute Kenilworth, NJ 07033 908-740-4669 ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From NMargaryan <@t> childrensmemorial.org Wed Mar 1 12:59:27 2006 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Wed Mar 1 12:58:39 2006 Subject: [Histonet] CY-90 monoclonal Message-ID: <63B8B599DE283148B92E83C78B32C15D01C762BA@cmhexbe2.childrensmemorial.org> Hi all, Is anybody out there doing IHC using of the CY-90 monoclonal antibody from Sigma on mouse tissues? If so, please send me a protocol. Any suggestions would be greatly appreciated. Thanks, Naira Naira V. Margaryan, D.V.M., Ph.D. Research Associate III Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3394 Tel: 773-755-6570/ext-8 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 From Rachael_Emerson <@t> URMC.Rochester.edu Wed Mar 1 13:09:41 2006 From: Rachael_Emerson <@t> URMC.Rochester.edu (Emerson, Rachael) Date: Wed Mar 1 13:10:08 2006 Subject: [Histonet] ICAM 1 questions Message-ID: Hello. Has anyone worked with ICAM-1 antibody before? Specifically I am working with goat anti-mouse ICAM-1 from Serotec (sc-1511) and I am having trouble getting it to work, as well as, trouble finding existing articles. I would really appreciate some suggestions. Thank you Rachael Emerson From emry <@t> u.washington.edu Wed Mar 1 13:24:42 2006 From: emry <@t> u.washington.edu (Trisha Emry) Date: Wed Mar 1 13:24:46 2006 Subject: [Histonet] Botox study Message-ID: <000501c63d65$ca5558e0$8c775f80@sod.washington.edu> Hi Netters, My boss is writing a grant and we are doing a pilot project looking at the possible effects of using Botox. It is being used for chronic pain for tmj problems, chronic pelvic pain in men and for bladder problems in women not to mention the wrinkle treatments. She has asked me to get some information on nerve tracers (?), neurolabeling (?) and stains that can be used to show if there are changes in the nerves with the use of Botox. I have No experience with this. I could use some direction on where to look for basic information? Vendor information would be helpful too. Thanks for your time, Trisha U of Washington Seattle From DDittus787 <@t> aol.com Wed Mar 1 13:35:54 2006 From: DDittus787 <@t> aol.com (DDittus787@aol.com) Date: Wed Mar 1 13:36:51 2006 Subject: [Histonet] Botox study Message-ID: <266.69b1595.3137519a@aol.com> Trish; Hi I am the product manager for polysciences. There are some great and easy to use neuronal tracer dyes like our Fast Blue . Feel free to check this product on our website _www.polysciences.com_ (http://www.polysciences.com) or call us to get more information at 1-800-523-2575. Dana Dittus Life Sciences/Histology Product Manager Polysciences,Inc. _ddittus@polysciences.com_ (mailto:ddittus@polysciences.com) From LuckG <@t> empirehealth.org Wed Mar 1 14:41:59 2006 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Wed Mar 1 14:42:09 2006 Subject: [Histonet] FW: A/P Manager Position in Boston Message-ID: Hello all, Thought I'd share this email to me for those of you who weren't aware of the opening, are qualified and looking for a very challenging opportunity you can sink your teeth into this one. Warmly, Greg Greg Luck, BS, HT(ASCP) Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 Phone 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmedicalcenter.org _____ From: Deborah Regen [mailto:Deborah@labcareer.com] Sent: Wednesday, March 01, 2006 10:33 AM To: luckg@empirehealth.org Subject: A/P Manager Position in Boston Dear Greg, We have a new opening with one of our clients in the Boston area for an A/P lab manager, salary range from $58K - $88K plus relo possible sign-on bonus. Would you know of someone to refer to us, for a $500 bonus paid to you if we place the person you recommend? Sincerely Yours, Deborah Regen Recruiter HealthCare Connections, Inc. Toll Free: # 1-866-346-8522 deborah@labcareer.com www.labcareer.com From Rcartun <@t> harthosp.org Wed Mar 1 15:00:26 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Mar 1 15:01:11 2006 Subject: [Histonet] Polioma virus - antibody Message-ID: I think they are referring to polyomavirus. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> Heather Palmer-Renko 02/28/06 04:53PM >>> In regards to a known diagnostic antibody specific to the polioma virus I don't believe one exist and I would imagine it would be an ASR if so, in addition I know that there has been some research on polioma and they made up their own poly-clonal cocktail. I am sure you already know this but, your first step would be doing a FISH for renal carcinoma to rule that out-I am sure you do. I am VERY familiar with the Ventana XT platform and know that if you could find a concentrate somewhere it could work-have you tried zymed? I will try to look through some literature tonight see what I can find. Good luck! Heather Renko, B.S., HT(ASCP) --------------------------------- Brings words and photos together (easily) with PhotoMail - it's free and works with Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LuckG <@t> empirehealth.org Wed Mar 1 15:08:29 2006 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Wed Mar 1 15:08:35 2006 Subject: [Histonet] C.P.T. CODING (RE: Decals) Message-ID: Hello all, Quick question. How should "Decal's" (i.e. CPT code 88311) be assessed. My interpretation is that the code is an add on and should be per specimen in conjunction with it's overlying primary surgical pathology code rather than per case. It seems to me it's written in somewhat ambiguous language in the CPT coding manual and I'd be interested in input either definitive or your current/prior operational practice. Thanks, Greg Greg Luck, BS, HT(ASCP) Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 Phone 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org From JWEEMS <@t> sjha.org Wed Mar 1 15:10:05 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Mar 1 15:10:10 2006 Subject: [Histonet] C.P.T. CODING (RE: Decals) Message-ID: <83AACDB0810528418AA106F9AE9B7F7E013059B2@sjhaexc02.sjha.org> It is 1 per specimen. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Luck, Greg D. Sent: Wednesday, March 01, 2006 4:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] C.P.T. CODING (RE: Decals) Hello all, Quick question. How should "Decal's" (i.e. CPT code 88311) be assessed. My interpretation is that the code is an add on and should be per specimen in conjunction with it's overlying primary surgical pathology code rather than per case. It seems to me it's written in somewhat ambiguous language in the CPT coding manual and I'd be interested in input either definitive or your current/prior operational practice. Thanks, Greg Greg Luck, BS, HT(ASCP) Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 Phone 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From sandosis <@t> uia.net Wed Mar 1 15:34:48 2006 From: sandosis <@t> uia.net (sandosis@uia.net) Date: Wed Mar 1 15:34:56 2006 Subject: [Histonet] Bone Marrow Decaling for IHC Message-ID: <200603012134.k21LYnq09648@smtp3.uia.net> Hello, Does anyone have sugestions for a "gentle" yet "effective" method of decaling bone marrow cores so that the antigenic sites remain viable? Thank you, Sandy PS Thanks for all the response on clearing colon fat to reveal lymph nodes, we are going to try the PenFix first as we are already set for the haz. waste disposal for that reagent. --------------------------------------------- This message was sent using the UIA Web Mail Server. ULTIMATE Internet Access, Inc http://www.uia.net/ From LuckG <@t> empirehealth.org Wed Mar 1 15:56:10 2006 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Wed Mar 1 15:56:19 2006 Subject: FW: [Histonet] Revealing lymph nodes Message-ID: -----Original Message----- From: Luck, Greg D. Sent: Wednesday, March 01, 2006 1:42 PM To: 'Marshall Terry Dr, Consultant Histopathologist' Subject: RE: [Histonet] Revealing lymph nodes Oh it's always something, We use PenFix from R.A. with marginal improvement in helping distinguish lymph nodes from surrounding tissues (obviously "fat" in particular). If you're willing to tolerate a bit more obnoxious dissection experience add a little Trichloroacetic Acid to your Davidson's or try some Tri-Fix which we have custom made for us by American Mastertech. It's nearly as effective as "Disect-Aid" but less unpleasant to work with. I know how impressed many of our surgeons are when we're able to consistently identify and demonstrate lymph nodes from those problematic (from a node shuckin' perspective) low anterior and abdominal perineal resections. Good luck, Greg Greg Luck, BS, HT(ASCP) Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 Phone 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmedicalcenter.org -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: Wednesday, March 01, 2006 5:10 AM To: rsrichmond@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Revealing lymph nodes Sigh. This is an opportune discussion, because I have tried Davidson's in the past and could see no difference whatsoever between before and after. In the last few days we have made up a new batch, modified slightly to take into consideration JK's view that there should be a greater proportion of alcohol. Same result, no difference whatsoever. No clearing, no lymph nodes going white - nothing. Is this the Emperor's new cloths? PS I only tried it in the first place because of the regard I have for anything uttered by Bob Richmond. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: rsrichmond@aol.com [mailto:rsrichmond@aol.com] Sent: 28 February 2006 18:58 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Revealing lymph nodes I think that a clearing fixative (such as the examples already given; there are also commercial products) is essential for finding the small lymph nodes that are often positive for colon cancer in colon resection specimens. Remember that just one tiny positive lymph node upstages the tumor, and makes chemotherapy mandatory - so there's a real patient care decision involved here. In my travels as a locum tenens pathologist I noted that about half of pathologists were using clearing fixatives, and about half were not. As far as I know there have been no official pronouncements on the subject. Clearing fixatives are less necessary in axillary dissection material for breast cancer - the specimensare smaller and the lymph nodes - and metastases - are usually much bigger. The mesenteries from a colon cancer resection are voluminous and hard to search through. Bob Richmond Gastonia NC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Wed Mar 1 16:03:15 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Mar 1 16:03:19 2006 Subject: [Histonet] Revealing lymph nodes Message-ID: <83AACDB0810528418AA106F9AE9B7F7E013059B6@sjhaexc02.sjha.org> We use Clark's - alcohol and acetic acid - Carnoy's without the chloroform. It does fairly well.. j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Luck, Greg D. Sent: Wednesday, March 01, 2006 4:56 PM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] Revealing lymph nodes -----Original Message----- From: Luck, Greg D. Sent: Wednesday, March 01, 2006 1:42 PM To: 'Marshall Terry Dr, Consultant Histopathologist' Subject: RE: [Histonet] Revealing lymph nodes Oh it's always something, We use PenFix from R.A. with marginal improvement in helping distinguish lymph nodes from surrounding tissues (obviously "fat" in particular). If you're willing to tolerate a bit more obnoxious dissection experience add a little Trichloroacetic Acid to your Davidson's or try some Tri-Fix which we have custom made for us by American Mastertech. It's nearly as effective as "Disect-Aid" but less unpleasant to work with. I know how impressed many of our surgeons are when we're able to consistently identify and demonstrate lymph nodes from those problematic (from a node shuckin' perspective) low anterior and abdominal perineal resections. Good luck, Greg Greg Luck, BS, HT(ASCP) Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 Phone 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmedicalcenter.org -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: Wednesday, March 01, 2006 5:10 AM To: rsrichmond@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Revealing lymph nodes Sigh. This is an opportune discussion, because I have tried Davidson's in the past and could see no difference whatsoever between before and after. In the last few days we have made up a new batch, modified slightly to take into consideration JK's view that there should be a greater proportion of alcohol. Same result, no difference whatsoever. No clearing, no lymph nodes going white - nothing. Is this the Emperor's new cloths? PS I only tried it in the first place because of the regard I have for anything uttered by Bob Richmond. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: rsrichmond@aol.com [mailto:rsrichmond@aol.com] Sent: 28 February 2006 18:58 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Revealing lymph nodes I think that a clearing fixative (such as the examples already given; there are also commercial products) is essential for finding the small lymph nodes that are often positive for colon cancer in colon resection specimens. Remember that just one tiny positive lymph node upstages the tumor, and makes chemotherapy mandatory - so there's a real patient care decision involved here. In my travels as a locum tenens pathologist I noted that about half of pathologists were using clearing fixatives, and about half were not. As far as I know there have been no official pronouncements on the subject. Clearing fixatives are less necessary in axillary dissection material for breast cancer - the specimensare smaller and the lymph nodes - and metastases - are usually much bigger. The mesenteries from a colon cancer resection are voluminous and hard to search through. Bob Richmond Gastonia NC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From liz <@t> premierlab.com Wed Mar 1 16:24:59 2006 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Mar 1 16:25:08 2006 Subject: [Histonet] Bone Marrow Decaling for IHC In-Reply-To: <200603012134.k21LYnq09648@smtp3.uia.net> Message-ID: <000601c63d7e$fa44bbe0$95d48a80@Chlipala> Sandy We don't do a lot of bone marrow cores, but we run a lot of immunos on rat, mouse and goat joints. I find that formic acid works well, we use a concentration of 5 to 10%. For antigen retreival I like to use proteinase K or other enzymatic methods. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sandosis@uia.net Sent: Wednesday, March 01, 2006 2:35 PM To: histonet@lists.utsouthwestern.edu Cc: mark.lones@stjoe.edu Subject: [Histonet] Bone Marrow Decaling for IHC Hello, Does anyone have sugestions for a "gentle" yet "effective" method of decaling bone marrow cores so that the antigenic sites remain viable? Thank you, Sandy PS Thanks for all the response on clearing colon fat to reveal lymph nodes, we are going to try the PenFix first as we are already set for the haz. waste disposal for that reagent. --------------------------------------------- This message was sent using the UIA Web Mail Server. ULTIMATE Internet Access, Inc http://www.uia.net/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1.1423 (20060301) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From dmccaig <@t> ckha.on.ca Wed Mar 1 16:23:48 2006 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Wed Mar 1 16:26:20 2006 Subject: [Histonet] hepatitis control block Message-ID: Greetings Does anyone have a positive control block that will show hepatitis in the orcein stain that they would be willing to share with me? It would be truly appreciated. Diana McCaig, MLT From clarke.ian <@t> virgin.net Wed Mar 1 16:33:29 2006 From: clarke.ian <@t> virgin.net (Ian Clarke) Date: Wed Mar 1 16:33:52 2006 Subject: [Histonet] Re Florida vacation Message-ID: Hi all, Sorry this is not solely histo but i am going to orlando in june/july and was wondering if any of our American colleagues could suggest if it would be better for me to purchase attraction tickets to disney from here in UK or perhaps could suggest a source for these.Also is sat nav a good idea. Thanks in advance for the advice Ian From rjbuesa <@t> yahoo.com Wed Mar 1 16:52:03 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 1 16:52:12 2006 Subject: [Histonet] Bone Marrow Decaling for IHC In-Reply-To: <200603012134.k21LYnq09648@smtp3.uia.net> Message-ID: <20060301225203.84818.qmail@web61219.mail.yahoo.com> Sandy: We always used EDTA and antigenic sites were well preserved. Ren? J. sandosis@uia.net wrote: Hello, Does anyone have sugestions for a "gentle" yet "effective" method of decaling bone marrow cores so that the antigenic sites remain viable? Thank you, Sandy PS Thanks for all the response on clearing colon fat to reveal lymph nodes, we are going to try the PenFix first as we are already set for the haz. waste disposal for that reagent. --------------------------------------------- This message was sent using the UIA Web Mail Server. ULTIMATE Internet Access, Inc http://www.uia.net/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Mail Bring photos to life! New PhotoMail makes sharing a breeze. From amylee779 <@t> yahoo.com Wed Mar 1 17:01:42 2006 From: amylee779 <@t> yahoo.com (Amy Lee) Date: Wed Mar 1 17:01:46 2006 Subject: [Histonet] Thanks a lot! Message-ID: <20060301230142.1226.qmail@web37402.mail.mud.yahoo.com> Thank you! I received a lot of responses. I forwarded them all to my boss and I am so happy we will have a pathologist soon. Thanks again! Amy --------------------------------- Yahoo! Mail Bring photos to life! New PhotoMail makes sharing a breeze. From Lchausse <@t> nmh.org Wed Mar 1 21:04:58 2006 From: Lchausse <@t> nmh.org (Chaussey, Leslie) Date: Wed Mar 1 21:05:29 2006 Subject: [Histonet] Great opportunity for the right person! References: Message-ID: We currently have a full-time Technical Coordinator position open in the Surgical Pathology Laboratory. Bachelors degree in appropriate science required & five years of relevant laboratory experience, Supervisory experience preferred, Proficient histotechnologist with superior problem solving skills desired. Oversees technical workflow in a busy Histology and IHC laboratory. Acts under the direction of the manager; the Technical Coordinator is responsible for facilitating the delivery of quality service for the Histology, IHC, Neuropath, and Electron Microscopy laboratories (75% technical coordinator / 25% staff bench work); Works with assigned departments and pathologists to coordinate assessment, development, and implementation of new technology; Designs data collection and assesssment systems; Assists with and monitors operating budget; Responsibilities include maintaining the highest degree of expertise in new technology, quality management, and the LIS. Northwestern Memorial Hospital, consistently recognized as the most preferred hospital in Chicago, is seeking new team members. We hire the 'Best People' who are dedicated to being a part of the 'Best Patient Experience!' At Northwestern Memorial Hospital, we're setting new standards of care. We're also creating career opportunities that allow you to share your talent, develop new skills, and reach all of your goals. We're one of the best hospitals in the world because we support the efforts, the ideas, and the initiatives of all of our people. We invite you to join us. For more information and to submit your resume, please visit our website, www.nmh.org . AA/EOE ----------------------------------------- This message and any included attachments are intended only for the addressee. The information contained in this message is confidential and may constitute proprietary or non-public information under international, federal, or state laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail. From omnivore98 <@t> yahoo.com Wed Mar 1 21:22:54 2006 From: omnivore98 <@t> yahoo.com (Heather Palmer-Renko) Date: Wed Mar 1 21:22:59 2006 Subject: [Histonet] Re: Mohs technician needed-Arlington Hts, Illinois Message-ID: <20060302032254.87253.qmail@web31302.mail.mud.yahoo.com> If anyone knows of someone or is interested in doing MOHS work on Monday and Tuesdays from 8am to 12-1pm for a great dermatologists in a small practice please reply to me via email. Great docter-good compensation-nice leica cryostat! Heather Renko, BS, HT(ASCP) --------------------------------- Yahoo! Mail Bring photos to life! New PhotoMail makes sharing a breeze. From nair.ashwin <@t> gmail.com Wed Mar 1 22:09:11 2006 From: nair.ashwin <@t> gmail.com (Ashwin Nair) Date: Wed Mar 1 22:09:15 2006 Subject: [Histonet] BCA Assay on Polymer Scaffolds Message-ID: Hi, I was wondering if anybody has done a BCA assay on polymeric scaffolds to detect the presence of protein in the scaffold. I have been usu=ing Coomassie Brilliant Blue currently but the staining is not very good as the background itslef is bluish no matter how long i destain it. Was wondering if BCA assay would be a better idea. I intend to do the assay on the slides which have these scaffold sections. I look forward to comments. Regards Ashwin Nair From jnocito <@t> satx.rr.com Thu Mar 2 05:38:27 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Mar 2 05:38:31 2006 Subject: [Histonet] C.P.T. CODING (RE: Decals) References: Message-ID: <001d01c63ded$d2b79d30$e8bd0b43@yourxhtr8hvc4p> Greg, you are correct. It is an additional charge. However, be careful. If 3 decal blocks are submitted on one specimen, you can only charge 1 88311. If there are 3 different specimens, and all get decaled, you can charge for 3 88311. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Luck, Greg D." To: Sent: Wednesday, March 01, 2006 3:08 PM Subject: [Histonet] C.P.T. CODING (RE: Decals) > Hello all, > Quick question. How should "Decal's" (i.e. CPT code 88311) be assessed. > My > interpretation is that the code is an add on and should be per specimen in > conjunction with it's overlying primary surgical pathology code rather > than > per case. It seems to me it's written in somewhat ambiguous language in > the CPT coding manual and I'd be interested in input either definitive or > your current/prior operational practice. Thanks, Greg > Greg Luck, BS, HT(ASCP) > Anatomic Pathology Supervisor > Deaconess Medical Center > 800 W. 5th Ave > Spokane, WA 99204 > Phone 509.473.7077 > Fax 509.473.7133 > luckg@empirehealth.org > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.375 / Virus Database: 268.1.1/271 - Release Date: 2/28/2006 > > From petepath <@t> yahoo.com Thu Mar 2 07:08:11 2006 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Thu Mar 2 07:08:15 2006 Subject: [Histonet] FNA'S Message-ID: <20060302130811.5900.qmail@web30401.mail.mud.yahoo.com> Few tips that have worked for me. On our FNA carts we keep a simple H&E set up as we would in the frozen room,and a diff quick set up. I find a very key factor is to quickly make the smears before it begins to clot. I find my besy chance of this is to immediately express all the material on one slide ( if it fits) and the quickly devide it by touching the puddle with one or more additional slides, quickly smearing it and getting it right ino 95% ETOH or air drying. When you get very bloody passes quickly tilt up the slide, run the blood off and pick up the small remaining pieces and smear them on a other slide.This will give you blood free smears. Let the blood clot and put it in formalin for a cell block. Hope fully you have a pathologist that will come down for a quick assessment.You should also have RPMI for flow cytometry and ask for another pass if lymphoma is suspected. Keep some small formalin bottles and tissue or filter bags to wrap small fragments or cores that you will sometimes get which are suitable for histology. When I see the initial sample and suspect I will need immunos to make a diagnosis or suggest a primary site for a met to the liver for example, I ask for a core biopsy or an aggressive bloody pass. I then blow this out on a slide , let it clot and use it as a cell block. I never hesitate to ask for additional passes if I question the adequacy of the material. Let the radiologist will tell you when it is time to quit. Cruel fact, with the exception of an occasional suprise in the cell block, if the material is of questionable adequacy when I am in radiology or the frozen section room, ofter it remains inadequate when you get it back to the lab! Do not let radiologists and surgeons intimidate you. They will be very happy to share the blame for a second procedure with the pathologist. Good luck Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com From kemlo.rogerson <@t> waht.swest.nhs.uk Thu Mar 2 09:03:36 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Mar 2 09:04:01 2006 Subject: [Histonet] FNA'S Message-ID: We tend to air dry our FNAs and to look at one for ROSE (rapid onsite evaluation) to gauge adequacy. The air dried sample is stained with Diff-Quick and looked at onsite. I tend not to make 'blood films' as that disrupts cells, especially oat cells, and you end up with debris, smear cells, etc. I quickly (very quickly) deposit a spot of the fluid onto a slide and touch the face of another clean slide flat on top with the slides at 90 degrees to each other with NO shearing motion; the slides are then pulled apart. They are then dried; these touch slides tend not to have any artefact caused mechanically. You can fix rather than air dry when you pull the slides apart with a spray fix. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 "Following the line of least resistance makes both men and rivers crooked" -----Original Message----- From: Stephen Peters M.D. [mailto:petepath@yahoo.com] Sent: Thursday, March 02, 2006 1:08 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] FNA'S Few tips that have worked for me. On our FNA carts we keep a simple H&E set up as we would in the frozen room,and a diff quick set up. I find a very key factor is to quickly make the smears before it begins to clot. I find my besy chance of this is to immediately express all the material on one slide ( if it fits) and the quickly devide it by touching the puddle with one or more additional slides, quickly smearing it and getting it right ino 95% ETOH or air drying. When you get very bloody passes quickly tilt up the slide, run the blood off and pick up the small remaining pieces and smear them on a other slide.This will give you blood free smears. Let the blood clot and put it in formalin for a cell block. Hope fully you have a pathologist that will come down for a quick assessment.You should also have RPMI for flow cytometry and ask for another pass if lymphoma is suspected. Keep some small formalin bottles and tissue or filter bags to wrap small fragments or cores that you will sometimes get which are suitable for histology. When I see the initial sample and suspect I will need immunos to make a diagnosis or suggest a primary site for a met to the liver for example, I ask for a core biopsy or an aggressive bloody pass. I then blow this out on a slide , let it clot and use it as a cell block. I never hesitate to ask for additional passes if I question the adequacy of the material. Let the radiologist will tell you when it is time to quit. Cruel fact, with the exception of an occasional suprise in the cell block, if the material is of questionable adequacy when I am in radiology or the frozen section room, ofter it remains inadequate when you get it back to the lab! Do not let radiologists and surgeons intimidate you. They will be very happy to share the blame for a second procedure with the pathologist. Good luck Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> Mercer.edu Thu Mar 2 09:27:36 2006 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Thu Mar 2 09:28:20 2006 Subject: [Histonet] Bone Marrow Decaling for IHC In-Reply-To: <200603012134.k21LYnq09648@smtp3.uia.net> Message-ID: <01LZKR2LKFUC8WYHOP@Macon2.Mercer.edu> I recommend using 5% formic acid. Although in the past I did IHC on bones decaled in RDO with very nice results. Be sure you have adequate fixation first. Shirley Powell -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sandosis@uia.net Sent: Wednesday, March 01, 2006 4:35 PM To: histonet@lists.utsouthwestern.edu Cc: mark.lones@stjoe.edu Subject: [Histonet] Bone Marrow Decaling for IHC Hello, Does anyone have sugestions for a "gentle" yet "effective" method of decaling bone marrow cores so that the antigenic sites remain viable? Thank you, Sandy PS Thanks for all the response on clearing colon fat to reveal lymph nodes, we are going to try the PenFix first as we are already set for the haz. waste disposal for that reagent. --------------------------------------------- This message was sent using the UIA Web Mail Server. ULTIMATE Internet Access, Inc http://www.uia.net/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SARAH.REEVES <@t> ekht.nhs.uk Thu Mar 2 09:37:07 2006 From: SARAH.REEVES <@t> ekht.nhs.uk (SARAH REEVES) Date: Thu Mar 2 09:38:12 2006 Subject: [Histonet] Alcian Blue deposit Message-ID: Hi Does anyone have any ideas to reduce alcian blue deposit on sections. We filter the solution after making up as well as immediatly before use. Thanks Sarah ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of East Kent Hospitals NHS Trust. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you have received this email in error please notify the system manager at the following email address: root.postmaster@ekht.nhs.uk www.kentandmedway.nhs.uk This footnote also confirms that although this email message has been swept by MIMEsweeper for the presence of computer viruses, it is strongly recommended that you carry out your own virus scan of this message and any attachments. www.mimesweeper.com ********************************************************************** From petepath <@t> yahoo.com Thu Mar 2 11:40:37 2006 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Thu Mar 2 11:40:44 2006 Subject: Fwd: RE: [Histonet] FNA'S Message-ID: <20060302174037.28639.qmail@web30407.mail.mud.yahoo.com> Hi Rogerson I believe there is a great deal of information that can be learned only from the smearing of cells that cannot be seen in paraffin histology and more importantly in the newer liquid medium methods which do not smear cells. As you mentioned, the crushing nuclei of small cell carcinoma and many other small blue cell tumors, also referred to a Azzopardi Phenomenon" is an important diagnostic clue. How a cell responds to the pressure of smearing tells us a lot about the structural integrity of the cell. Imagine smearing a jellyfish and a turtle under a giant slide. I think cells have similar structural differences which would yield equally different results. Epithelial cells, which function as structural lining and evolved to withstand such stressed tend to be more tightly cohesive and less prone to give up naked nuclei. Cells such as endocrine cells, whose role is less structural and involved in secretory function tend to have more delicate cytoplasm easily disrupt and give off many naked nuclei. Neuroendocrine tumors also fall into this category. Just the breast changes that go on with lactation result in much more delicate cells and numerous naked nuclei. Dense fibrous lesions are often very reluctant to smearing. A fragment of large cell lymphoma can ce confused with poorly differentiated carcinomas on a thin prep but when smeared, the lack of cohesion is a strong clue. When I get a spinal lesion with a differential of menengioma versus schwannoma, I can usually tell simply by how the tissue reacts when making the smears. A schwannoma will role under the slide like a piece of rubber band, and the menengioma often smears with relative ease and surprising discohesion. I feel quite strongly about this matter and believe we are making a mistake by taking Non medical cytology and squirting them in a jar of fixative, to have the cells sucked onto a slide without smearing. I won't mention any names! From lmusunuri <@t> yahoo.com Thu Mar 2 11:52:41 2006 From: lmusunuri <@t> yahoo.com (Lakshmi musunuri) Date: Thu Mar 2 11:52:47 2006 Subject: [Histonet] about stained tissue used for protein assay In-Reply-To: <20060301153328.72147.qmail@web54315.mail.yahoo.com> Message-ID: <20060302175241.28857.qmail@web54301.mail.yahoo.com> I have just started working on rabbit heart tissue and I am developing assays to study proteins through western blots. These rabbit tissues are stained with Evans blue and Triphenyl tetrazolium chloride (TTC)to study regional ishcemia. The problem I have is that when I try to perform a protein quantitation using a Bradford or BCA assay, these dyes interfere with the absorption readings. I request you to kindly inform me what method can be used to correct the error in the absorption caused by the dyes. The amount of dye that these tissues can take up is not known and hence I am not able to apply a particular value to correct the error. I have tried various protein qauntification methods but at all these wavelengths there is some absorbance by these dyes. It would be of great help if you could let me know the details about the protein quantification method that would be best to follow. Or atleast how to perform a correction for the background values given by these dyes. Thanking you, Srilaxmi Musunuri --------------------------------- Brings words and photos together (easily) with PhotoMail - it's free and works with Yahoo! Mail. From Terry.Marshall <@t> rothgen.nhs.uk Thu Mar 2 12:07:37 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Thu Mar 2 12:07:37 2006 Subject: [Histonet] FNA'S Message-ID: Much of what you say is right, but I don't think you can tell anything from a smear cell that is not a leap of faith. BTW I understand the Azzopardi phenomenon to refer to the DNA staining of blood vessels in oat cell carcinoma (all right then, small cell carcinoma). Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Stephen Peters M.D. [mailto:petepath@yahoo.com] Sent: 02 March 2006 17:41 To: Histonet@lists.utsouthwestern.edu Subject: Fwd: RE: [Histonet] FNA'S Hi Rogerson I believe there is a great deal of information that can be learned only from the smearing of cells that cannot be seen in paraffin histology and more importantly in the newer liquid medium methods which do not smear cells. As you mentioned, the crushing nuclei of small cell carcinoma and many other small blue cell tumors, also referred to a Azzopardi Phenomenon" is an important diagnostic clue. How a cell responds to the pressure of smearing tells us a lot about the structural integrity of the cell. Imagine smearing a jellyfish and a turtle under a giant slide. I think cells have similar structural differences which would yield equally different results. Epithelial cells, which function as structural lining and evolved to withstand such stressed tend to be more tightly cohesive and less prone to give up naked nuclei. Cells such as endocrine cells, whose role is less structural and involved in secretory function tend to have more delicate cytoplasm easily disrupt and give off many naked nuclei. Neuroendocrine tumors also fall into this category. Just the breast changes that go on with lactation result in much more delicate cells and numerous naked nuclei. Dense fibrous lesions are often very reluctant to smearing. A fragment of large cell lymphoma can ce confused with poorly differentiated carcinomas on a thin prep but when smeared, the lack of cohesion is a strong clue. When I get a spinal lesion with a differential of menengioma versus schwannoma, I can usually tell simply by how the tissue reacts when making the smears. A schwannoma will role under the slide like a piece of rubber band, and the menengioma often smears with relative ease and surprising discohesion. I feel quite strongly about this matter and believe we are making a mistake by taking Non medical cytology and squirting them in a jar of fixative, to have the cells sucked onto a slide without smearing. I won't mention any names! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NSEARCY <@t> swmail.sw.org Thu Mar 2 12:07:55 2006 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Thu Mar 2 12:08:11 2006 Subject: [Histonet] Powerpath Users Message-ID: Am purchasing the imaging system. What scanners do you recommend? Anyone from the U of Washington out there? I emailed Kim Simmons but have not heard from her. Thanks From modernarnis73 <@t> juno.com Thu Mar 2 12:09:42 2006 From: modernarnis73 <@t> juno.com (modernarnis73@juno.com) Date: Thu Mar 2 12:10:43 2006 Subject: [Histonet] Barrier Slides Message-ID: <20060302.100959.26452.514651@webmail47.lax.untd.com> Hello Everyone, I am looking for barrier slides, similiar to the Biogenex / optiplus 1/3 microscope slides. I would appreciate any suggestions. Enoch ________________________________________________________________________ Try Juno Platinum for Free! Then, only $9.95/month! Unlimited Internet Access with 1GB of Email Storage. Visit http://www.juno.com/value to sign up today! From godsgirlnow <@t> msn.com Thu Mar 2 12:13:40 2006 From: godsgirlnow <@t> msn.com (Roxanne Soto) Date: Thu Mar 2 12:13:47 2006 Subject: [Histonet] Barrier Slides In-Reply-To: <20060302.100959.26452.514651@webmail47.lax.untd.com> Message-ID: Try Biocare.... Roxanne Soto ______________________________________________________________ From: "modernarnis73@juno.com" To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Barrier Slides Date: Thu, 2 Mar 2006 18:09:42 GMT >Hello Everyone, >I am looking for barrier slides, similiar to the Biogenex / optiplus 1/3 microscope slides. >I would appreciate any suggestions. >Enoch > > > >__________________________________________________________________ ______ >Try Juno Platinum for Free! Then, only $9.95/month! >Unlimited Internet Access with 1GB of Email Storage. >Visit http://www.juno.com/value to sign up today! >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Thu Mar 2 12:36:49 2006 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Mar 2 12:35:59 2006 Subject: [Histonet] Alcian Blue deposit In-Reply-To: References: Message-ID: <44073B41.1090405@uwo.ca> If an alcian blue solution contains insoluble material, or if insoluble material forms after filtering, the solution must be no good. Solutions at pH 1.0 or pH 2.5 can be stable for some years. Solutions of alcian blue used in "critical electrolyte concentration" procedures are stable for only a few hours. See Horobin 2002 - Ch.26 in Conn's Biological Stains, 10th edn. Insoluble blue material is almost certainly copper phthalocyanine pigment. The books say this dissolves in concentrated sulphuric acid, but it can't be removed with any ordinary solvent. Alcian blue has been available as a certified dye powder since 1981, so use only a certified batch. The solid dye powder can sometimes deteriorate; this may be related to dye purity, with certain non-dye additives (notably boric acid) improving stability. Generally, solid dyes are stable for many years. A related dye is the alcian blue pyridine variant (Sigma-Aldrich), which is more stable and reliable than alcian blue (see Churukian, Frank & Horobin, 2000 Biotech. Histochem. 75: 147-150; Henwood 2002 Biotech. Histochem. 77: 93-94.) With the pyridine variant you do not obtain a completely acid-resistant product like that produced by staining with real alcian blue, but that may not matter for many purposes. Another more stable alcian blue substitute is alcec blue; I don't know if this is still available. The most recent alcec blue publication (of very few) that I've seen is a nicely illustrated 2001 paper on sulfoglycolipid storage disease by Lullmann-Rauch et al, in Histochemistry & Cell Biology 116:161-169. -- ------------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan[AT]uwo.ca http://publish.uwo.ca/~jkiernan/ http://instruct.uwo.ca/anatomy/530/index.htm _______________________________ SARAH REEVES wrote: > Hi > > Does anyone have any ideas to reduce alcian blue deposit on sections. We filter the solution after making up as well as immediatly before use. > > Thanks > Sarah > www.kentandmedway.nhs.uk > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------- From LuckG <@t> empirehealth.org Thu Mar 2 12:41:06 2006 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Thu Mar 2 12:41:15 2006 Subject: [Histonet] Alcian Blue deposit Message-ID: Suggestion, Might try laying the slides down flat, cover with a slightly moist (HOH) sheet of filter paper (e.g. #40's Whatman) and saturate filter paper with your Alcian Blue working solution for your standard staining time. That's what we do to solve a similar problem with our Gram stain. Good luck, Greg -----Original Message----- From: SARAH REEVES [mailto:SARAH.REEVES@ekht.nhs.uk] Sent: Thursday, March 02, 2006 7:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Alcian Blue deposit Hi Does anyone have any ideas to reduce alcian blue deposit on sections. We filter the solution after making up as well as immediatly before use. Thanks Sarah ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of East Kent Hospitals NHS Trust. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you have received this email in error please notify the system manager at the following email address: root.postmaster@ekht.nhs.uk www.kentandmedway.nhs.uk This footnote also confirms that although this email message has been swept by MIMEsweeper for the presence of computer viruses, it is strongly recommended that you carry out your own virus scan of this message and any attachments. www.mimesweeper.com ********************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From petepath <@t> yahoo.com Thu Mar 2 12:49:32 2006 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Thu Mar 2 12:49:39 2006 Subject: [Histonet] FNA'S Message-ID: <20060302184932.62402.qmail@web30403.mail.mud.yahoo.com> Hi Terry, I agree with you about smear cells. Coincidently, yesterday a colleague brought me a case he thought was oat cell. Nuclei were typical neuroendocrine salt and pepper, but there was no smearing at all, and many nice intact clusters. I was very reluctant to agree with oat cell and questioned whether it was a somewhat better differentiated neuroendocrine tumor or even an adeno masquerading. I think the absence of this can also be a clue. I remember the smearing being referred to as Azzopardi Phenomenon in my training but now that you mention it I have seen the changes you referred to under this name. I stand corrected. Stephen From jerry.santiago <@t> jax.ufl.edu Thu Mar 2 13:06:38 2006 From: jerry.santiago <@t> jax.ufl.edu (Santiago, Jerry) Date: Thu Mar 2 13:06:42 2006 Subject: [Histonet] BRCA-1 & BRCA-2 Antibodies Message-ID: Hello Netters, I need help with BRCA-1 & BRCA-2 Antibodies. I am using BRCA-1 from Dako and BRCA-2 from LabVision I can't get them to work. Can anyone using these antibodies share their method that is helping them get these to work. Thanks in advance, Jerry Santiago Pathology Technologist Shands Jacksonville jerry.santiago@jax.ufl.edu 904-244-6149 From dharclerode <@t> cytoritx.com Thu Mar 2 13:10:50 2006 From: dharclerode <@t> cytoritx.com (Donna Harclerode) Date: Thu Mar 2 13:09:44 2006 Subject: [Histonet] ICAM-1, CD54 Message-ID: <3DE0F644E093DF4BAE80C254176696A508FB78@mp-mailserver.macropore.com> Hi Rachael I am not familiar with the Serotech antibody, but PharMingen has an anti mouse CD54 (ICAM-1) made in Armenian hamster link http://www.bdbiosciences.com/external_files/pm/doc/tds/ihc/live/web_enab led/74121E_550287.pdf that works great in frozen mouse sections at 5ug/ml. You do need to purchase the Armenian hamster secondary for this antibody cat 550335 (biotinolated anti Armenian hamster) and use a LSAB, ABC or other tertiary with DAB. PharMingen makes a lot of antibodies directed against mice (humans and rats too), mostly CD markers, that work very well in IHC. If you are looking for any other specific targets, let me know and I can check my data sheets for you (I generated data in 97 for all the PharMingen antibodies that were available then for IHC applications). I could also send the sheets to you if you wish. The catalog numbers have all changed since 97, but the clones are still the same. Good luck, Donna Harclerode, HT, (ASCP), HTL, QIHC Scientist / Immunohistochemistry Cytori Therapeutics 3020 Callan Rd. San Diego, CA 92121 858-458-0900 ext 322 dharclerode@cytoritx.com From sandosis <@t> uia.net Thu Mar 2 13:52:13 2006 From: sandosis <@t> uia.net (sandosis@uia.net) Date: Thu Mar 2 13:52:20 2006 Subject: [Histonet] Thank you! Message-ID: <200603021952.k22JqEl14251@smtp2.uia.net> Thanks for all the input on decaling bone marrows, we are going to give RapidCal-Immuno a try. Sandy --------------------------------------------- This message was sent using the UIA Web Mail Server. ULTIMATE Internet Access, Inc http://www.uia.net/ From rsrichmond <@t> aol.com Thu Mar 2 16:01:57 2006 From: rsrichmond <@t> aol.com (rsrichmond@aol.com) Date: Thu Mar 2 16:02:23 2006 Subject: [Histonet] Holde Puchtler - obituary In-Reply-To: <200603021253.2bb440731271e2@rly-yh03.mx.aol.com> References: <200603021253.2bb440731271e2@rly-yh03.mx.aol.com> Message-ID: <8C80C5C0EF5D238-A90-1D40@FWM-R06.sysops.aol.com> I just received word from a friend in the pathology department of the Medical College of Georgia (at Augusta GA) that Dr. Holde Puchtler has died at the age of 86. I don't have any other obituary information now, not even a date of death, but will post any I can obtain. I would expect an obituary to appear in the Augusta Chronicle. Many of you will know that Dr. Puchtler, often with Susan N. Meloan and Faye Sweat Waldrop, published very numerous articles (PubMed lists 96) in histochemistry, on such topics as Congo red staining for amyloid, fluorescent dyes for fungi, her "methacarn" fixative, and many other things - I have a thick folder of reprints from her but can't get hold of it at the moment. Dr. Puchtler was postlingually deaf, and communicated in writing. I tried to arrange a meeting with her while I was doing some locum tenens work at MCG, but it didn't work out. Bob Richmond Gastonia NC From RSRICHMOND <@t> aol.com Thu Mar 2 20:34:26 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Thu Mar 2 20:34:41 2006 Subject: [Histonet] Revealing lymph nodes Message-ID: <2bc.5fdc198.31390532@aol.com> Several correspondents report having trouble getting clearing fixatives to work. Let's look at methods. (For people new to this issue - we're talking about fixation methods that render lymph nodes opaque while making the surrounding adipose tissue clear - for finding lymph nodes in cancer resection specimens.) 1. Overnight fixation is needed. 2. You need an adequate volume of fixative for the amount of tissue to be fixed, and that can be quite a lot. 3. Before you put the fatty mesenteries in the fixative, you have to slice them thin enough for the fixative to penetrate them. It's helpful to stir the container after an hour or so of fixation. 4. Looking for lymph nodes in the fixed tissue remains time-consuming, and it requires good ventilation. I've successfully used Davidson's fixative (three parts water, three parts ethanol, two parts 37% formaldehyde [strong formalin, not buffered], and one part glacial acetic acid. Of commercial products I've had good results with one called O-Fix, not so good with at least one of the others. In the immortal words of Herman Melville's Moby-Dick, chapter 95: "mincing the horse-pieces of blubber for the pots; an operation which is conducted at a curious wooden horse, planted endwise against the bulwarks, and with a capacious tub beneath it, into which the minced pieces drop.... Bible leaves! Bible leaves! This is the invariable cry from the mates to the mincer. It enjoins him to be careful, and cut his work into as thin slices as possible, inasmuch as by so doing the business of boiling out the oil is much accelerated, and its quantity considerably increased, besides perhaps improving it in quality." Bob Richmond Gastonia NC From cpomajzl <@t> cpllabs.com Thu Mar 2 22:17:50 2006 From: cpomajzl <@t> cpllabs.com (Chris Pomajzl) Date: Thu Mar 2 22:15:39 2006 Subject: [Histonet] HT Salary Survey Message-ID: <001f01c63e79$6faef620$26fca8c0@CSP> I'm trying to get an idea of the salary ranges for an experienced histotech. I am evaluating our payscale, and I need to know where we fall in the overall scheme. Most of our techs make between $20-24 per hour with occasional overtime, and shift-diff for a few early employees. Can you guys give me a rough estimate for what you make/pay for HT's with or without ASCP certification. Let's just say someone with 5-10 years of experience. We are still looking for histotechs that would like to work in the beautiful Hill Country of Austin Texas. If you are interested, please let me know. Thanks in advance for all responses. Sincerely, Chris Pomajzl, HTL (ASCP) Histology Supervisor Clinical Pathology Laboratories, Inc. Austin, Texas 512.873.1660 (o) 512.873.5004 (f) cpomajzl@cpllabs.com CONFIDENTIALITY NOTICE: This communication is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If you are not the intended recipient, you are notified that any use, dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this message in error, please notify the sender immediately and destroy this communication. From Tanni.Ahmed <@t> intervet.com Fri Mar 3 02:27:05 2006 From: Tanni.Ahmed <@t> intervet.com (Ahmed, T (Tanni)) Date: Fri Mar 3 02:28:06 2006 Subject: [Histonet] fertility/sterility staining Message-ID: <11DBF8AFF1DC0F4A9B51A23F8E5BDA333AD7CE@mksn83.d50.intra> Hi all, One of our pathologists has asked if a histology stain is available to assess the sterility/fertility of small animals i.e rabbits (staining epididymis, sperm), does anyone know of any stains/protocols? Is the Nigrosin-eosin (Hancock) stain used? Thanks in advance, Tanni -------------------------------------- This message, including attachments, is confidential and may be privileged. If you are not an intended recipient, please notify the sender then delete and destroy the original message and all copies. You should not copy, forward and/or disclose this message, in whole or in part, without permission of the sender. -------------------------------------- From kemlo.rogerson <@t> waht.swest.nhs.uk Fri Mar 3 02:38:10 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Mar 3 02:38:25 2006 Subject: [Histonet] FNA'S Message-ID: Maybe that is the problem on relying on an artefact to aid diagnosis? My point is how you know if the artefact is just absent for an undisclosed reason rather than the cells being unable biologically to submit themselves. I am no expert but IMHO it must be better science to make sure what you see reflects in vivo cells as much as is practicable. Despite the cells being dead, dried, fixed, stained, um............. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 "Following the line of least resistance makes both men and rivers crooked" This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Stephen Peters M.D. [mailto:petepath@yahoo.com] Sent: Thursday, March 02, 2006 6:50 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] FNA'S Hi Terry, I agree with you about smear cells. Coincidently, yesterday a colleague brought me a case he thought was oat cell. Nuclei were typical neuroendocrine salt and pepper, but there was no smearing at all, and many nice intact clusters. I was very reluctant to agree with oat cell and questioned whether it was a somewhat better differentiated neuroendocrine tumor or even an adeno masquerading. I think the absence of this can also be a clue. I remember the smearing being referred to as Azzopardi Phenomenon in my training but now that you mention it I have seen the changes you referred to under this name. I stand corrected. Stephen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo.rogerson <@t> waht.swest.nhs.uk Fri Mar 3 04:09:39 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Mar 3 04:09:52 2006 Subject: [Histonet] HT Salary Survey Message-ID: Trainee Biomedical Scientist 65%to 75% of either ?24,000 or ?30,000. Biomedical Scientist Entry Level ?18,698 to ?24,198 Biomedical Scientist 'experienced' ?22,328 to ?30,247 Biomedical Scientist 'specialist' ?25,628 to ?35,527 Biomedical Scientist 'Professional Manager' ?34,372 to ?41,246 Biomedical Scientist 'Professional Manager'/ Pathology Manager ? 40,036 to ?49,496 Biomedical Scientist Pathology Manager/ Consultant ?48,176 to ?59,395 Biomedical Scientist Consultant ?57,745 to ?71,494 Biomedical Scientist Director of Service ?68,194 to ?86,240 ($143,000 ish) Roughly. May not travel well to the USA (1.67 dollars to the pound)but 'bitter' drinkers will understand. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 "Following the line of least resistance makes both men and rivers crooked" This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Chris Pomajzl [mailto:cpomajzl@cpllabs.com] Sent: Friday, March 03, 2006 4:18 AM To: HISTONET Subject: [Histonet] HT Salary Survey I'm trying to get an idea of the salary ranges for an experienced histotech. I am evaluating our payscale, and I need to know where we fall in the overall scheme. Most of our techs make between $20-24 per hour with occasional overtime, and shift-diff for a few early employees. Can you guys give me a rough estimate for what you make/pay for HT's with or without ASCP certification. Let's just say someone with 5-10 years of experience. We are still looking for histotechs that would like to work in the beautiful Hill Country of Austin Texas. If you are interested, please let me know. Thanks in advance for all responses. Sincerely, Chris Pomajzl, HTL (ASCP) Histology Supervisor Clinical Pathology Laboratories, Inc. Austin, Texas 512.873.1660 (o) 512.873.5004 (f) cpomajzl@cpllabs.com CONFIDENTIALITY NOTICE: This communication is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If you are not the intended recipient, you are notified that any use, dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this message in error, please notify the sender immediately and destroy this communication. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From petepath <@t> yahoo.com Fri Mar 3 06:28:05 2006 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Fri Mar 3 06:28:09 2006 Subject: [Histonet] FNA'S In-Reply-To: Message-ID: <20060303122805.44304.qmail@web30408.mail.mud.yahoo.com> Morning Rogerson, The best way I can explain it is when you have seen hundreds of dogs in your life you learn what they look like and how they behave. Then one day you are pretty sure you saw a dog fly over the roof of your house. One should be pretty sure before you decide it is a flying dog. It is a lot more likely that it was a large bat! Reading cytology is a lot like reading surg path through a pinhole. You use all of your experience and instincts to put a puzzle together in your mind. By diagnosing an oat cell without any smearing one would be missing an important piece of the puzzle and without it you may be looking at a large bat. Do my pathologist colleagues disagree? Kemlo Rogerson wrote: Maybe that is the problem on relying on an artefact to aid diagnosis? My point is how you know if the artefact is just absent for an undisclosed reason rather than the cells being unable biologically to submit themselves. I am no expert but IMHO it must be better science to make sure what you see reflects in vivo cells as much as is practicable. Despite the cells being dead, dried, fixed, stained, um............. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 "Following the line of least resistance makes both men and rivers crooked" This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Stephen Peters M.D. [mailto:petepath@yahoo.com] Sent: Thursday, March 02, 2006 6:50 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] FNA'S Hi Terry, I agree with you about smear cells. Coincidently, yesterday a colleague brought me a case he thought was oat cell. Nuclei were typical neuroendocrine salt and pepper, but there was no smearing at all, and many nice intact clusters. I was very reluctant to agree with oat cell and questioned whether it was a somewhat better differentiated neuroendocrine tumor or even an adeno masquerading. I think the absence of this can also be a clue. I remember the smearing being referred to as Azzopardi Phenomenon in my training but now that you mention it I have seen the changes you referred to under this name. I stand corrected. Stephen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgirlnow <@t> msn.com Fri Mar 3 06:38:40 2006 From: godsgirlnow <@t> msn.com (Roxanne Soto) Date: Fri Mar 3 06:38:50 2006 Subject: [Histonet] CLIA REGS Message-ID: Would someone from CLIA (or someone who knows the CLIA regs inside and out) please contact regarding grossing of tissue-----what is grossing per se--does measuring cores and putting them in a cassette count as grossing? What education level doesn one have to have in order to do this in the state of Florida? We are in desperate need of lab aides, but our lab aides have always had BS degrees and they "gross" out prostate cores....... Thanks in advance Roxanne Soto From jotahal <@t> ftvs.cuni.cz Fri Mar 3 07:17:59 2006 From: jotahal <@t> ftvs.cuni.cz (=?iso-8859-2?Q?Jakub_Ot=E1hal?=) Date: Fri Mar 3 07:18:27 2006 Subject: [Histonet] organotypic hippocampal cultures and immunohistochemistry Message-ID: <006201c63ec4$e7d70b60$0601a8c0@d333jotahal> Hi Histoneters, I have problem with immunohistochemistry on organotypic hippocampal slice cultures (OHSC). I have stained them with OX-42(CD11b) and glucoseoxidase-DAB development but the staining is weak and unsharp. When I tried the same protocol on rat brain slices the staining is very nice. OHSC are fixed for 1hour in 4% PFA then 20%sucroze and then glued on freezing stage and cut with microtome to 30mikron slices. Does anybody have an idea where is the bug? Thank you very much Best regards jakub -------------------------------------------------------------------------- Jakub Otahal MD,PhD Department of Developemental Epileptology Institute of Physiology Czech Academy of Sciences Videnska 1083 142 20 Prague 4 Czech Republic tel: +420 241062495 jotahal@ftvs.cuni.cz ------------------------------------------------------------------------- From kemlo.rogerson <@t> waht.swest.nhs.uk Fri Mar 3 07:59:48 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Mar 3 08:00:00 2006 Subject: [Histonet] FNA'S Message-ID: That is of course the Cytologists instinctive response; that we create in our mind a library of evidence that we use to diagnose the abnormality. Whilst I have for years accepted that I don't think its 'good science'. If I may use your analogy, there are many varieties of dogs as there are abnormal cells. The trick I suppose is to recognise the variety as it may be useful to know if the animal that confronts you is a Pekinese (unlikely to kill you) or a Pit Bull on supercharge (very likely to kill you); I'm getting to like this analogy. If you camouflage the Pit Bull that's fine if all Pit Bulls are camouflaged; if not you are in trouble, especially if it learnt to fly. Sorry to labour the point but I think introducing artefact as an aid to identification is poor science; IMHO. With respect why just ask your Pathologist colleagues? Don't we all have an input into Diagnostic Cytology; well we do in the UK. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 "Following the line of least resistance makes both men and rivers crooked" This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Stephen Peters M.D. [mailto:petepath@yahoo.com] Sent: Friday, March 03, 2006 12:28 PM To: Kemlo Rogerson; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] FNA'S Morning Rogerson, The best way I can explain it is when you have seen hundreds of dogs in your life you learn what they look like and how they behave. Then one day you are pretty sure you saw a dog fly over the roof of your house. One should be pretty sure before you decide it is a flying dog. It is a lot more likely that it was a large bat! Reading cytology is a lot like reading surg path through a pinhole. You use all of your experience and instincts to put a puzzle together in your mind. By diagnosing an oat cell without any smearing one would be missing an important piece of the puzzle and without it you may be looking at a large bat. Do my pathologist colleagues disagree? Kemlo Rogerson wrote: Maybe that is the problem on relying on an artefact to aid diagnosis? My point is how you know if the artefact is just absent for an undisclosed reason rather than the cells being unable biologically to submit themselves. I am no expert but IMHO it must be better science to make sure what you see reflects in vivo cells as much as is practicable. Despite the cells being dead, dried, fixed, stained, um............. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 "Following the line of least resistance makes both men and rivers crooked" This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Stephen Peters M.D. [mailto:petepath@yahoo.com] Sent: Thursday, March 02, 2006 6:50 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] FNA'S Hi Terry, I agree with you about smear cells. Coincidently, yesterday a colleague brought me a case he thought was oat cell. Nuclei were typical neuroendocrine salt and pepper, but there was no smearing at all, and many nice intact clusters. I was very reluctant to agree with oat cell and questioned whether it was a somewhat better differentiated neuroendocrine tumor or even an adeno masquerading. I think the absence of this can also be a clue. I remember the smearing being referred to as Azzopardi Phenomenon in my training but now that you mention it I have seen the changes you referred to under this name. I stand corrected. Stephen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ncoolen <@t> burns.nl Fri Mar 3 08:27:01 2006 From: ncoolen <@t> burns.nl (Coolen, Neeltje) Date: Fri Mar 3 08:27:07 2006 Subject: [Histonet] Ki-67 on skin tissue Message-ID: <8A67A7CA4D84D44CB1B8F3D657CE599E3004D7@server-02.brandwonden.local> Dear Histonetters, I am trying to get Ki-67 clone Ki-S5 (labVision/Neomarkers) working on FFPE skin tissue. I have tried HIER in citrate buffer pH 6.0 and in Tris/EDTA buffer pH 9.0, but this does not work. Does anyone have an idea? Thank you. Neeltje Coolen -------------------------------------- N.A. Coolen, PhD student VSBN Zeestraat 29 1941 AJ Beverwijk The Netherlands tel: +31 251 275 564 From failm <@t> musc.edu Fri Mar 3 08:35:20 2006 From: failm <@t> musc.edu (Mildred Fail) Date: Fri Mar 3 08:35:45 2006 Subject: [Histonet] Ki-67 on skin tissue Message-ID: We did get that clone to work but not as well or as consistently as Ki-67 clone K3 from Cell Marque. The dilution 1:50 with HIER in EDTA Rena Fail >>> "Coolen, Neeltje" 03/03/06 09:27AM >>> Dear Histonetters, I am trying to get Ki-67 clone Ki-S5 (labVision/Neomarkers) working on FFPE skin tissue. I have tried HIER in citrate buffer pH 6.0 and in Tris/EDTA buffer pH 9.0, but this does not work. Does anyone have an idea? Thank you. Neeltje Coolen -------------------------------------- N.A. Coolen, PhD student VSBN Zeestraat 29 1941 AJ Beverwijk The Netherlands tel: +31 251 275 564 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ebreisch <@t> chsd.org Fri Mar 3 08:48:13 2006 From: Ebreisch <@t> chsd.org (Breisch, Eric) Date: Fri Mar 3 08:45:43 2006 Subject: [Histonet] Histology Position Message-ID: <00D7298B3825D511BF540008C75F8A3C0CAD82A7@excluster.chsd.org> Hi Histonetters, I am notifying any and all who might have an interest in coming to Children's Hospital and Health Center in San Diego, CA. We have an immediate opening for a histology technician in the Dept. of Pathology/ Division of Histology. We have a full service laboratory that handles routine histology, special stains, IFA, IHC, and muscle histochemistry. We are a relatively small but busy lab. Depending upon your motivation and interest there is also an opportunity to learn electron microscopy. Children's Hospital offers a competitive salary package commensurate with experience and full medical benefits with a retirement package. San Diego is a beautiful community to live and work in with great recreational opportunities. Send all inquiries to the contact listed below. Regards, Eric A. Breisch, Ph.D. Clinical Anatomist Histology Supervisor Dept. of Pathology MC5007 3020 Children's Way CHHC San Diego, CA 92123 858-966-5944 ebreisch@chsd.org Associate Clinical Professor of Anatomy UCSD SOM La Jolla, CA From Charles.Embrey <@t> carle.com Fri Mar 3 09:09:50 2006 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Fri Mar 3 09:09:27 2006 Subject: FW: [Histonet] CLIA REGS Message-ID: -----Original Message----- From: Charles.Embrey Sent: Friday, March 03, 2006 9:09 AM To: 'Roxanne Soto' Subject: RE: [Histonet] CLIA REGS CLIA '88 lists the requirements for non-pathologists grossing. Grossing is considered high-complexity testing even if it's a punch biopsy or a shave. CLIA '88 states "On or before April 24 1995 (I) be a high school graduate or equivalent; and (b) have documentation of training appropriate for the test performed before analyzing patient specimens"................After that date it requires an associate degree in a biological or chemical science or medical laboratory technology -or- qualify as a medical technologist with a bachelor's degree from an accredited institution -or- earned a bachelor's degree in a chemical, physical, biologic or clinical laboratory science. ref. CLIA '88 493.1489 As to your question: "Does measuring cores and putting them in a cassette Count as grossing?" YES it does. Whether a simple small skin tag or dissection of an entire colon, the requirements are the same. It falls under the CLIA High Complexity Testing Personnel Qualifications, Federal Register VOl. 60, No. 78, April 1995, section 493.1489 Also CAP requires a written instruction detailing what specimens may be grossed with direct vs indirect pathologists' observation. Direct means that the pathologist literally watches over your shoulder while you gross the specimen. Indirect means that he is readily available to consult. Now as far a Florida is concerned: Florida has one of the most stringent licensing systems in the US. I fully expect, now that Pathologists' Assistants are a certified fact, that Florida will look closely at their licensure and may limit grossing in the state to licensed P.A.s, Pathologists and residents. At this point it is just a guess but I wouldn't be surprised to see it happen in the not too distant future. Charles Embrey, PA(ASCP) Carle Clinic Urbana, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roxanne Soto Sent: Friday, March 03, 2006 6:39 AM To: HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] CLIA REGS Would someone from CLIA (or someone who knows the CLIA regs inside and out) please contact regarding grossing of tissue-----what is grossing per se--does measuring cores and putting them in a cassette count as grossing? What education level doesn one have to have in order to do this in the state of Florida? We are in desperate need of lab aides, but our lab aides have always had BS degrees and they "gross" out prostate cores....... Thanks in advance Roxanne Soto _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SBarnes <@t> elch.org Fri Mar 3 10:11:16 2006 From: SBarnes <@t> elch.org (Sue Barnes) Date: Fri Mar 3 10:11:20 2006 Subject: [Histonet] meditech user Message-ID: Hi, I am looking for someone who has Meditech as their LIS. I would like to have a response from someone in the Microbiology department. Any help would be greatly appreciated. Sue Barnes East Liverpool City Hospital East Liverpool, Ohio Phone: 330 386-2968 From gpbnas <@t> yahoo.es Fri Mar 3 11:05:07 2006 From: gpbnas <@t> yahoo.es (Guillermo Palao) Date: Fri Mar 3 11:05:11 2006 Subject: [Histonet] Ki67 on mouse kidney tissue Message-ID: <20060303170507.51889.qmail@web26207.mail.ukl.yahoo.com> Hello all, Does anybody recommend a particular rabbit anti-Ki67 Ab that works well in mouse kidney? I have seen many different commercial Abs but I would like some first-hand information before buying one. Thanks in advance, Guillermo Palao, MD. Ph.D. Laboratorio de Reumatolog?a Centro de Investigaci?n Hospital 12 de Octubre Avda de C?rdoba s/n Madrid 28041 Spain --------------------------------- LLama Gratis a cualquier PC del Mundo. Llamadas a fijos y m?viles desde 1 c?ntimo por minuto. http://es.voice.yahoo.com From Terry.Marshall <@t> rothgen.nhs.uk Fri Mar 3 11:09:15 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Mar 3 11:09:16 2006 Subject: [Histonet] Revealing lymph nodes Message-ID: Bob, I hear you and heed you as I have heard and heeded you before. My last outing was of a mere 25 Grams of axillary fat in which I had found only 3 lymph nodes. This spoilt the record for our breast surgeons, so I was requested to go back and find at least another one. The measly mass had already been bread sliced, so I put it in about 300 mls of Davidson's and left it 24 hours. I found the one extra lymph node, but it was no thanks to the Davidson's which had had no observable effect. Am I not holding my feet right? :-) Dr Terry (still thinking it's a con) L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: RSRICHMOND@aol.com [mailto:RSRICHMOND@aol.com] Sent: 03 March 2006 02:34 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Revealing lymph nodes Several correspondents report having trouble getting clearing fixatives to work. Let's look at methods. (For people new to this issue - we're talking about fixation methods that render lymph nodes opaque while making the surrounding adipose tissue clear - for finding lymph nodes in cancer resection specimens.) 1. Overnight fixation is needed. 2. You need an adequate volume of fixative for the amount of tissue to be fixed, and that can be quite a lot. 3. Before you put the fatty mesenteries in the fixative, you have to slice them thin enough for the fixative to penetrate them. It's helpful to stir the container after an hour or so of fixation. 4. Looking for lymph nodes in the fixed tissue remains time-consuming, and it requires good ventilation. I've successfully used Davidson's fixative (three parts water, three parts ethanol, two parts 37% formaldehyde [strong formalin, not buffered], and one part glacial acetic acid. Of commercial products I've had good results with one called O-Fix, not so good with at least one of the others. In the immortal words of Herman Melville's Moby-Dick, chapter 95: "mincing the horse-pieces of blubber for the pots; an operation which is conducted at a curious wooden horse, planted endwise against the bulwarks, and with a capacious tub beneath it, into which the minced pieces drop.... Bible leaves! Bible leaves! This is the invariable cry from the mates to the mincer. It enjoins him to be careful, and cut his work into as thin slices as possible, inasmuch as by so doing the business of boiling out the oil is much accelerated, and its quantity considerably increased, besides perhaps improving it in quality." Bob Richmond Gastonia NC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> aol.com Fri Mar 3 11:23:12 2006 From: rsrichmond <@t> aol.com (rsrichmond@aol.com) Date: Fri Mar 3 11:23:20 2006 Subject: [Histonet] Revealing lymph nodes In-Reply-To: <200603031210.62e4408789436d@rly-xn02.mx.aol.com> References: <200603031210.62e4408789436d@rly-xn02.mx.aol.com> Message-ID: <8C80CFE4860E334-17E4-722E@mblk-r37.sysops.aol.com> Terry Marshall notes having had no success with revealing lymph nodes by post-fixation in Davidson's fixative. Neither have I. The clearing fixative must be used as the initial fixative. An old method is to pass the formalin-fixed tissue through alcohols into xylene (or cedar oil). I've never used this method, and the one time I've seen it used it didn't work. Once in a while there are no more than three lymph nodes in the axillary contents from a breast cancer. Your surgeon should live with it. Bob Richmond From TJJ <@t> Stowers-Institute.org Fri Mar 3 11:42:06 2006 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Fri Mar 3 11:42:27 2006 Subject: [Histonet] RE: Ki67 on mouse kidney tissue Message-ID: We use the rabbit monoclonal from Labvision: Citrate Buffer HIER 10' in a microwave @ 95 degrees C, cool 10' Nonserum block - 10' Ki67 1:200 - 1 hour @ RT or overnight @ 4 degrees C Immunovision rabbit polymer HRP - 30 minutes @ RT DAB+ Others use Proteinase K enzyme digestion with this antibody with good results. Best wishes, Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64133 From dusko.trajkovic <@t> pfizer.com Fri Mar 3 12:13:08 2006 From: dusko.trajkovic <@t> pfizer.com (Trajkovic, Dusko) Date: Fri Mar 3 12:13:16 2006 Subject: [Histonet] Ki67 on mouse kidney tissue Message-ID: <3AD0BD3142459B4E9B12CBEAFF2B89B2027EFC9F@lajamrexm01.amer.pfizer.com> I also use a Rabbit Monoclonal Ki-67 from Lab Vision. Works great. On Ventana Discovery XT: DAB Map Kit / CC1 Standard / Primary at 1:800 / GaR (Dako) 1:200. Dusko trajkovic -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Guillermo Palao Sent: Friday, March 03, 2006 9:05 AM To: Histonet Subject: [Histonet] Ki67 on mouse kidney tissue Hello all, Does anybody recommend a particular rabbit anti-Ki67 Ab that works well in mouse kidney? I have seen many different commercial Abs but I would like some first-hand information before buying one. Thanks in advance, Guillermo Palao, MD. Ph.D. Laboratorio de Reumatolog?a Centro de Investigaci?n Hospital 12 de Octubre Avda de C?rdoba s/n Madrid 28041 Spain --------------------------------- LLama Gratis a cualquier PC del Mundo. Llamadas a fijos y m?viles desde 1 c?ntimo por minuto. http://es.voice.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately. From mtitford <@t> aol.com Fri Mar 3 12:27:02 2006 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Fri Mar 3 12:27:11 2006 Subject: [Histonet] Stain for sperm Message-ID: <8C80D07333EFB6C-248-A88@FWM-D31.sysops.aol.com> Tanni somewhere or the other asks if there is a stain for sperm. We have used the Berg method to stain sperm in prostate. The Berg stain is like a souped up Ziehl Neelsen. It is listed in Sheehans book, and may be on an Internet site somewhere. Mike Titford USA Pathology Mobile AL USA From michael.owen <@t> fda.hhs.gov Fri Mar 3 12:46:09 2006 From: michael.owen <@t> fda.hhs.gov (Owen, Michael P) Date: Fri Mar 3 12:46:39 2006 Subject: [Histonet] Stain for sperm Message-ID: Many years ago when working in a clinical laboratory that studied human sperm, I used the Diff-Quik and the Bryan-Leishman methods to stain sperm slides. The slides were analyzed by certified medical technologists. While looking on the Internet how to spell Bryan-Leishman, I came across the Web page listed below. The book mentioned on the Web page might be helpful to you although I have never read it. Oozoa Biomedical, Inc.: Practical Laboratory Andrology http://www.oozoa.com/publications_files/pla.htm Wishing you a great weekend, The FDA Lab Rat Michael P. Owen, Regulatory Microbiologist U.S. FDA Pacific Regional Lab Northwest 22201 23rd Drive SE Bothell, WA 98021-4421 Phone: 425-483-4865 E-Mail: michael.owen@fda.hhs.gov From LuckG <@t> empirehealth.org Fri Mar 3 12:47:45 2006 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Fri Mar 3 12:47:56 2006 Subject: [Histonet] CLIA REGS RE: Grossing: Chime In Message-ID: Charles, In answer to the question below about requirements for "non-pathologists grossing" you cited the specific text from CLIA '88 (493.1489) which states the requirements for "analyzing patient samples" but I don't see how that strictly translates to "grossing" (in particular when we may be talking about the simple transfer of an entire specimen into tissue cassettes with a visual description and simple specimen prep and set-up as in reducing the sizes of the sample to be processed; e.g. bisection with a scalpel. Wouldn't simple (where the entire specimen is submitted and no independent decision has to be made over what portions of the specimen to submit and/or not submit for micro exam by the pathologist be analogous for example to a Micro lab aid who does this on tissue cultures. "Grossing" by your comment below does not differentiate between shave biopsy and a prostatectomy. Where in CLIA does it's 'text' specifically state what constitutes "grossing" and who can or can not perform CLIA's definition of grossing. For those of us less familiar with the federal register can you provide me/us with the specific text from the federal register that you cite in your response to the 2nd question of "does measuring cores and placing them in a cassette count as grossing?" to which you have said yes it does. Thanks, Greg Greg Luck, BS, HT(ASCP) Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 Phone 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmedicalcenter.org -----Original Message----- From: Charles.Embrey [mailto:Charles.Embrey@carle.com] Sent: Friday, March 03, 2006 7:10 AM To: Histonet@pathology.swmed.edu Subject: FW: [Histonet] CLIA REGS -----Original Message----- From: Charles.Embrey Sent: Friday, March 03, 2006 9:09 AM To: 'Roxanne Soto' Subject: RE: [Histonet] CLIA REGS CLIA '88 lists the requirements for non-pathologists grossing. Grossing is considered high-complexity testing even if it's a punch biopsy or a shave. CLIA '88 states "On or before April 24 1995 (I) be a high school graduate or equivalent; and (b) have documentation of training appropriate for the test performed before analyzing patient specimens"................After that date it requires an associate degree in a biological or chemical science or medical laboratory technology -or- qualify as a medical technologist with a bachelor's degree from an accredited institution -or- earned a bachelor's degree in a chemical, physical, biologic or clinical laboratory science. ref. CLIA '88 493.1489 As to your question: "Does measuring cores and putting them in a cassette Count as grossing?" YES it does. Whether a simple small skin tag or dissection of an entire colon, the requirements are the same. It falls under the CLIA High Complexity Testing Personnel Qualifications, Federal Register VOl. 60, No. 78, April 1995, section 493.1489 Also CAP requires a written instruction detailing what specimens may be grossed with direct vs indirect pathologists' observation. Direct means that the pathologist literally watches over your shoulder while you gross the specimen. Indirect means that he is readily available to consult. Now as far a Florida is concerned: Florida has one of the most stringent licensing systems in the US. I fully expect, now that Pathologists' Assistants are a certified fact, that Florida will look closely at their licensure and may limit grossing in the state to licensed P.A.s, Pathologists and residents. At this point it is just a guess but I wouldn't be surprised to see it happen in the not too distant future. Charles Embrey, PA(ASCP) Carle Clinic Urbana, IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roxanne Soto Sent: Friday, March 03, 2006 6:39 AM To: HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] CLIA REGS Would someone from CLIA (or someone who knows the CLIA regs inside and out) please contact regarding grossing of tissue-----what is grossing per se--does measuring cores and putting them in a cassette count as grossing? What education level doesn one have to have in order to do this in the state of Florida? We are in desperate need of lab aides, but our lab aides have always had BS degrees and they "gross" out prostate cores....... Thanks in advance Roxanne Soto _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From king.laurie <@t> marshfieldclinic.org Fri Mar 3 13:39:03 2006 From: king.laurie <@t> marshfieldclinic.org (king.laurie@marshfieldclinic.org) Date: Fri Mar 3 13:39:01 2006 Subject: [Histonet] pneumatic tube system Message-ID: <37ade01c63efa$205734d0$8e0110ac@mfldclinframe.org> Good Afternoon All, I've recently been asked about issues with sending tissue specimens in formalin through a pneumatic tube system. For example, from an ambulatory surgery site to a lab on the other side of the building. Is anyone aware of specific regulations against the practice? Laurie King HT Eau Claire, WI From godsgirlnow <@t> msn.com Fri Mar 3 14:20:48 2006 From: godsgirlnow <@t> msn.com (Roxanne Soto) Date: Fri Mar 3 14:20:57 2006 Subject: [Histonet] CLIA REGS RE: Grossing: Chime In In-Reply-To: Message-ID: Greg, Thank you for your comment-that is exactly what I was trying to ask, but you put it so much better than I did. This is what I need to know, as well, and I have had a very difficult time trying to find the answer by looking at the CLIA website. Roxanne ______________________________________________________________ From: "Luck, Greg D." To: "'Charles.Embrey'" , Histonet@pathology.swmed.edu Subject: [Histonet] CLIA REGS RE: Grossing: Chime In Date: Fri, 3 Mar 2006 10:47:45 -0800 >Charles, >In answer to the question below about requirements for "non-pathologists >grossing" you cited the specific text from CLIA '88 (493.1489) which states >the requirements for "analyzing patient samples" but I don't see how that >strictly translates to "grossing" (in particular when we may be talking >about the simple transfer of an entire specimen into tissue cassettes with a >visual description and simple specimen prep and set-up as in reducing the >sizes of the sample to be processed; e.g. bisection with a scalpel. >Wouldn't simple (where the entire specimen is submitted and no independent >decision has to be made over what portions of the specimen to submit and/or >not submit for micro exam by the pathologist be analogous for example to a >Micro lab aid who does this on tissue cultures. "Grossing" by your comment >below does not differentiate between shave biopsy and a prostatectomy. >Where in CLIA does it's 'text' specifically state what constitutes >"grossing" and who can or can not perform CLIA's definition of grossing. >For those of us less familiar with the federal register can you provide >me/us with the specific text from the federal register that you cite in your >response to the 2nd question of "does measuring cores and placing them in a >cassette count as grossing?" to which you have said yes it does. Thanks, >Greg >Greg Luck, BS, HT(ASCP) >Anatomic Pathology Supervisor >Deaconess Medical Center >800 W. 5th Ave >Spokane, WA 99204 >Phone 509.473.7077 >Fax 509.473.7133 >luckg@empirehealth.org >www.deaconessmedicalcenter.org > > > > >-----Original Message----- >From: Charles.Embrey [mailto:Charles.Embrey@carle.com] >Sent: Friday, March 03, 2006 7:10 AM >To: Histonet@pathology.swmed.edu >Subject: FW: [Histonet] CLIA REGS > > > >-----Original Message----- >From: Charles.Embrey >Sent: Friday, March 03, 2006 9:09 AM >To: 'Roxanne Soto' >Subject: RE: [Histonet] CLIA REGS > >CLIA '88 lists the requirements for non-pathologists grossing. Grossing is >considered high-complexity testing even if it's a punch biopsy or a shave. > >CLIA '88 states "On or before April 24 1995 (I) be a high school graduate or >equivalent; and (b) have documentation of training appropriate for the test >performed before analyzing patient specimens"................After that date >it requires an associate degree in a biological or chemical science or >medical laboratory technology -or- qualify as a medical technologist with a >bachelor's degree from an accredited institution -or- earned a bachelor's >degree in a chemical, physical, biologic or clinical laboratory science. > >ref. CLIA '88 493.1489 > >As to your question: "Does measuring cores and putting them in a cassette > Count as grossing?" YES it does. Whether a simple small skin tag or >dissection of an entire colon, the requirements are the same. >It falls under the CLIA High Complexity Testing Personnel Qualifications, >Federal Register VOl. 60, No. 78, April 1995, section >493.1489 > >Also CAP requires a written instruction detailing what specimens may be >grossed with direct vs indirect pathologists' observation. Direct means that >the pathologist literally watches over your shoulder while you gross the >specimen. Indirect means that he is readily available to consult. > >Now as far a Florida is concerned: Florida has one of the most stringent >licensing systems in the US. I fully expect, now that Pathologists' >Assistants are a certified fact, that Florida will look closely at their >licensure and may limit grossing in the state to licensed P.A.s, >Pathologists and residents. At this point it is just a guess but I wouldn't >be surprised to see it happen in the not too distant future. > >Charles Embrey, PA(ASCP) >Carle Clinic >Urbana, IL > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roxanne Soto >Sent: Friday, March 03, 2006 6:39 AM >To: HISTONET@PATHOLOGY.SWMED.EDU >Subject: [Histonet] CLIA REGS > > > Would someone from CLIA (or someone who knows the CLIA regs inside > and out) please contact regarding grossing of tissue-----what is > grossing per se--does measuring cores and putting them in a cassette > count as grossing? What education level doesn one have to have >in > order to do this in the state of Florida? We are in desperate need of > lab aides, but our lab aides have always had BS degrees and they > "gross" out prostate cores....... > Thanks in advance > Roxanne Soto >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ian.montgomery <@t> bio.gla.ac.uk Fri Mar 3 14:25:49 2006 From: ian.montgomery <@t> bio.gla.ac.uk (Ian Montgomery) Date: Fri Mar 3 14:26:09 2006 Subject: [Histonet] Insects. Message-ID: <01e601c63f00$a9ca5fc0$532ed182@ibls.gla.ac.uk> At the beginning of February I asked for recommendations when processing insects with a chitin skeleton. Result and many thanks, it worked a treat, chloral hydrate and phenol. The chitin just slipped over the knife producing lovely ribbons. When asked how it was done, "ah, this is for the cognoscenti, a skill that takes years to develop." Again, many thanks. The original e-mails are on my laptop, you'll get full recognition in my techniques book and formulary. Ian. Dr. Ian Montgomery, Histotechnology, IBLS Support Unit, Graham Kerr Building, Tel: 4652, 6644. ian.montgomery@bio.gla.ac.uk From Lance.Erickson <@t> intermountainmail.org Fri Mar 3 15:10:36 2006 From: Lance.Erickson <@t> intermountainmail.org (Lance Erickson) Date: Fri Mar 3 15:10:49 2006 Subject: [Histonet] CLIA REGS RE: Grossing: Chime In Message-ID: <8B08EC394B366D4A895DD5686D6AFE4A09413E@LP-EXCHVS08.CO.IHC.COM> www.cms.hhs.gov/clia is the link to the CLIA '88 information. See the new federal register CLIA intrepretive guidelines appendix C subpart M that are effective April 24, 2003. The guidelines are available at www.cms.hhs.gov/CLIA/downloads/apcsubm.pdf under qualifications of high complexity testing personnel section 493.1489 (b)(7) it is clear that all tissue gross examination whether it is color, measurement, or advanced dissection is considered high complexity testing and individuals performing this type of testing must qualify under this section. That is why the new CAP question ANP 11610 was instated and is effective April 28, 2005. CAP must abide by CLIA regulations and CLIA is part of the Center for Medicare and Medicaid Services which is a section of the US government's department of Health and Human Services. So if you would like to maintain your CLIA license and CAP certification and be paid by Medicare you must abide by the requirements for high complexity testing personnel for each person performing any kind of gross examination. The actual wording is: "Interpretive Guidelines ?493.1489(b)(7)In the case of gross examinations, the technical supervisor may delegate to individuals qualified under ?493.1489 the responsibility for the physical examination/description, including color, weight, measurement and other characteristics of the tissue; or other mechanical procedures for which a specific written protocol has been developed. The technical supervisor is ultimately responsible for the diagnosis related to the gross examination and must sign the examination report. The technical supervisor is not required to provide direct onsite supervision but is responsible for the accuracy of all test results reported. All physical examinations/descriptions of tissue including color, weight, measurement and other characteristics of the tissue; or other mechanical procedures performed in the absence of the technical supervisor by individuals qualified under ?493.1489 should be reviewed within 24 hours by the technical supervisor. All microscopic tissue examinations must be performed by individuals qualified under ?493.1449(b), (l) or (m), as appropriate." Lance Erickson Anatomic Pathology Supervisor Primary Children's Medical Center Salt Lake City, UT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roxanne Soto Sent: Friday, March 03, 2006 1:21 PM To: LuckG@empirehealth.org; Charles.Embrey@carle.com; Histonet@pathology.swmed.edu Subject: RE: [Histonet] CLIA REGS RE: Grossing: Chime In Greg, Thank you for your comment-that is exactly what I was trying to ask, but you put it so much better than I did. This is what I need to know, as well, and I have had a very difficult time trying to find the answer by looking at the CLIA website. Roxanne ______________________________________________________________ From: "Luck, Greg D." To: "'Charles.Embrey'" , Histonet@pathology.swmed.edu Subject: [Histonet] CLIA REGS RE: Grossing: Chime In Date: Fri, 3 Mar 2006 10:47:45 -0800 >Charles, >In answer to the question below about requirements for "non-pathologists >grossing" you cited the specific text from CLIA '88 (493.1489) which states >the requirements for "analyzing patient samples" but I don't see how that >strictly translates to "grossing" (in particular when we may be talking >about the simple transfer of an entire specimen into tissue cassettes with a >visual description and simple specimen prep and set-up as in reducing the >sizes of the sample to be processed; e.g. bisection with a scalpel. >Wouldn't simple (where the entire specimen is submitted and no independent >decision has to be made over what portions of the specimen to submit and/or >not submit for micro exam by the pathologist be analogous for example to a >Micro lab aid who does this on tissue cultures. "Grossing" by your comment >below does not differentiate between shave biopsy and a prostatectomy. >Where in CLIA does it's 'text' specifically state what constitutes >"grossing" and who can or can not perform CLIA's definition of grossing. >For those of us less familiar with the federal register can you provide >me/us with the specific text from the federal register that you cite in your >response to the 2nd question of "does measuring cores and placing them in a >cassette count as grossing?" to which you have said yes it does. Thanks, >Greg >Greg Luck, BS, HT(ASCP) >Anatomic Pathology Supervisor >Deaconess Medical Center >800 W. 5th Ave >Spokane, WA 99204 >Phone 509.473.7077 >Fax 509.473.7133 >luckg@empirehealth.org >www.deaconessmedicalcenter.org > > > > >-----Original Message----- >From: Charles.Embrey [mailto:Charles.Embrey@carle.com] >Sent: Friday, March 03, 2006 7:10 AM >To: Histonet@pathology.swmed.edu >Subject: FW: [Histonet] CLIA REGS > > > >-----Original Message----- >From: Charles.Embrey >Sent: Friday, March 03, 2006 9:09 AM >To: 'Roxanne Soto' >Subject: RE: [Histonet] CLIA REGS > >CLIA '88 lists the requirements for non-pathologists grossing. Grossing is >considered high-complexity testing even if it's a punch biopsy or a shave. > >CLIA '88 states "On or before April 24 1995 (I) be a high school graduate or >equivalent; and (b) have documentation of training appropriate for the test >performed before analyzing patient specimens"................After that date >it requires an associate degree in a biological or chemical science or >medical laboratory technology -or- qualify as a medical technologist with a >bachelor's degree from an accredited institution -or- earned a bachelor's >degree in a chemical, physical, biologic or clinical laboratory science. > >ref. CLIA '88 493.1489 > >As to your question: "Does measuring cores and putting them in a cassette > Count as grossing?" YES it does. Whether a simple small skin tag or >dissection of an entire colon, the requirements are the same. >It falls under the CLIA High Complexity Testing Personnel Qualifications, >Federal Register VOl. 60, No. 78, April 1995, section >493.1489 > >Also CAP requires a written instruction detailing what specimens may be >grossed with direct vs indirect pathologists' observation. Direct means that >the pathologist literally watches over your shoulder while you gross the >specimen. Indirect means that he is readily available to consult. > >Now as far a Florida is concerned: Florida has one of the most stringent >licensing systems in the US. I fully expect, now that Pathologists' >Assistants are a certified fact, that Florida will look closely at their >licensure and may limit grossing in the state to licensed P.A.s, >Pathologists and residents. At this point it is just a guess but I wouldn't >be surprised to see it happen in the not too distant future. > >Charles Embrey, PA(ASCP) >Carle Clinic >Urbana, IL > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roxanne Soto >Sent: Friday, March 03, 2006 6:39 AM >To: HISTONET@PATHOLOGY.SWMED.EDU >Subject: [Histonet] CLIA REGS > > > Would someone from CLIA (or someone who knows the CLIA regs inside > and out) please contact regarding grossing of tissue-----what is > grossing per se--does measuring cores and putting them in a cassette > count as grossing? What education level doesn one have to have >in > order to do this in the state of Florida? We are in desperate need of > lab aides, but our lab aides have always had BS degrees and they > "gross" out prostate cores....... > Thanks in advance > Roxanne Soto >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dusko.trajkovic <@t> pfizer.com Fri Mar 3 15:14:28 2006 From: dusko.trajkovic <@t> pfizer.com (Trajkovic, Dusko) Date: Fri Mar 3 15:14:40 2006 Subject: [Histonet] Protocol for CD34 Message-ID: <3AD0BD3142459B4E9B12CBEAFF2B89B2027EFD25@lajamrexm01.amer.pfizer.com> Does anyone have a good protocol for CD 34 that works on FFPE rat tissue? Any information would be greatly appreciated. Dusko Trajkovic PGRD - W.W. Safety Sciences 1-858-638-6202 1-858-678-8290 fax dusko.trajkovic@pfizer.com 10646 Science Center Drive CB4 - 2154B San Diego, CA 92121 ---------------------------------------------------------------------- LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately. From MMcCOY <@t> lakelandregional.org Fri Mar 3 15:25:13 2006 From: MMcCOY <@t> lakelandregional.org (Mary McCoy) Date: Fri Mar 3 15:25:45 2006 Subject: [Histonet] Vendors that would deliver to Kabul Afghanistan Message-ID: Last fall, I went with "World Wide Lab Improvement" to Kabul, Afghanistan to help set up a pathology lab. At the time there was only one place (in Pakistan I believe) that they were able to get alcohols & xylene from. Does anyone in this great international community have any ideas as to vendors that would distribute histology processing chemicals to Afghanistan? They are having problems with the processing & a visiting pathologist thought it might be the quality of the reagents. I would appreciate any contacts or help any of you might have Thanks, Mary Mary McCoy Supervisor of Pathology Services Lakeland Regional Health System St. Joseph MI 49098 (269) 982-4891 -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:McCoy, Mary TEL;WORK:982-4891 ORG:;Lab TEL;PREF;FAX:98-8258 EMAIL;WORK;PREF;NGW:MMcCOY@lakelandregional.org N:McCoy;Mary TITLE:Coordinator Pathology END:VCARD BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:McCoy, Mary TEL;WORK:982-4891 ORG:;Lab TEL;PREF;FAX:98-8258 EMAIL;WORK;PREF;NGW:MMcCOY@lakelandregional.org N:McCoy;Mary TITLE:Coordinator Pathology TEL;PREF:982-4891 END:VCARD From Charles.Embrey <@t> carle.com Fri Mar 3 15:35:37 2006 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Fri Mar 3 15:35:45 2006 Subject: [Histonet] CLIA REGS RE: Grossing: Chime In Message-ID: Thanks to Lance Erickson the final answer is within reach. The new federal register CLIA interpretive guidelines appendix C subpart M effective April 24, 2003 states in section 493.1461(e) "In the case if gross examinations, the technical supervisor may delegate to individuals qualified under 493.1498 (This is the section I quoted in the first e-mail) the responsibility for the physical examination/description, including color, weight, measurement, and other characteristics of the tissue; or other mechanical procedures for which a specific written protocol has been developed." So under this interpretive guideline measuring cores and putting them into a cassette constitutes High Complexity Testing. You are right when you said, " "Grossing" by your comment below does not differentiate between shave biopsy and a prostatectomy." That is because in the eyes of CLIA there is no difference. Charles Embrey PA(ASCP) -----Original Message----- From: Roxanne Soto [mailto:godsgirlnow@msn.com] Sent: Friday, March 03, 2006 2:21 PM To: LuckG@empirehealth.org; Charles.Embrey; Histonet@pathology.swmed.edu Subject: RE: [Histonet] CLIA REGS RE: Grossing: Chime In Greg, Thank you for your comment-that is exactly what I was trying to ask, but you put it so much better than I did. This is what I need to know, as well, and I have had a very difficult time trying to find the answer by looking at the CLIA website. Roxanne ________________________________ From: "Luck, Greg D." To: "'Charles.Embrey'" , Histonet@pathology.swmed.edu Subject: [Histonet] CLIA REGS RE: Grossing: Chime In Date: Fri, 3 Mar 2006 10:47:45 -0800 >Charles, >In answer to the question below about requirements for "non-pathologists >grossing" you cited the specific text from CLIA '88 (493.1489) which states >the requirements for "analyzing patient samples" but I don't see how that >strictly translates to "grossing" (in particular when we may be talking >about the simple transfer of an entire specimen into tissue cassettes with a >visual description and simple specimen prep and set-up as in reducing the >sizes of the sample to be processed; e.g. bisection with a scalpel. >Wouldn't simple (where the entire specimen is submitted and no independent >decision has to be made over what portions of the specimen to submit and/or >not submit for micro exam by the pathologist be analogous for example to a >Micro lab aid who does this on tissue cultures. "Grossing" by your comment >below does not differentiate between shave biopsy and a prostatectomy. >Where in CLIA does it's 'text' specifically state what constitutes >"grossing" and who can or can not perform CLIA's definition of grossing. >For those of us less familiar with the federal register can you provide >me/us with the specific text from the federal register that you cite in your >response to the 2nd question of "does measuring cores and placing them in a >cassette count as grossing?" to which you have said yes it does. Thanks, >Greg >Greg Luck, BS, HT(ASCP) >Anatomic Pathology Supervisor >Deaconess Medical Center >800 W. 5th Ave >Spokane, WA 99204 >Phone 509.473.7077 >Fax 509.473.7133 >luckg@empirehealth.org >www.deaconessmedicalcenter.org > > > > >-----Original Message----- >From: Charles.Embrey [mailto:Charles.Embrey@carle.com] >Sent: Friday, March 03, 2006 7:10 AM >To: Histonet@pathology.swmed.edu >Subject: FW: [Histonet] CLIA REGS > > > >-----Original Message----- >From: Charles.Embrey >Sent: Friday, March 03, 2006 9:09 AM >To: 'Roxanne Soto' >Subject: RE: [Histonet] CLIA REGS > >CLIA '88 lists the requirements for non-pathologists grossing. Grossing is >considered high-complexity testing even if it's a punch biopsy or a shave. > >CLIA '88 states "On or before April 24 1995 (I) be a high school graduate or >equivalent; and (b) have documentation of training appropriate for the test >performed before analyzing patient specimens"................After that date >it requires an associate degree in a biological or chemical science or >medical laboratory technology -or- qualify as a medical technologist with a >bachelor's degree from an accredited institution -or- earned a bachelor's >degree in a chemical, physical, biologic or clinical laboratory science. > >ref. CLIA '88 493.1489 > >As to your question: "Does measuring cores and putting them in a cassette > Count as grossing?" YES it does. Whether a simple small skin tag or >dissection of an entire colon, the requirements are the same. >It falls under the CLIA High Complexity Testing Personnel Qualifications, >Federal Register VOl. 60, No. 78, April 1995, section >493.1489 > >Also CAP requires a written instruction detailing what specimens may be >grossed with direct vs indirect pathologists' observation. Direct means that >the pathologist literally watches over your shoulder while you gross the >specimen. Indirect means that he is readily available to consult. > >Now as far a Florida is concerned: Florida has one of the most stringent >licensing systems in the US. I fully expect, now that Pathologists' >Assistants are a certified fact, that Florida will look closely at their >licensure and may limit grossing in the state to licensed P.A.s, >Pathologists and residents. At this point it is just a guess but I wouldn't >be surprised to see it happen in the not too distant future. > >Charles Embrey, PA(ASCP) >Carle Clinic >Urbana, IL > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roxanne Soto >Sent: Friday, March 03, 2006 6:39 AM >To: HISTONET@PATHOLOGY.SWMED.EDU >Subject: [Histonet] CLIA REGS > > > Would someone from CLIA (or someone who knows the CLIA regs inside > and out) please contact regarding grossing of tissue-----what is > grossing per se--does measuring cores and putting them in a cassette > count as grossing? What education level doesn one have to have >in > order to do this in the state of Florida? We are in desperate need of > lab aides, but our lab aides have always had BS degrees and they > "gross" out prostate cores....... > Thanks in advance > Roxanne Soto >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bjdewe <@t> aol.com Fri Mar 3 15:45:37 2006 From: bjdewe <@t> aol.com (bjdewe@aol.com) Date: Fri Mar 3 15:45:47 2006 Subject: [Histonet] Smears revisited Message-ID: <8C80D22F16A2CB3-214-984@FWM-R03.sysops.aol.com> So, what is the best way to make smear slides from Lymphoma FNA's? I've seen several methods for different kinds of smears but not sure if they all work for every kind of condition? Lorie ******************************************************************* Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s)and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ******************************************************************* From kemlo <@t> f2s.com Sat Mar 4 07:24:22 2006 From: kemlo <@t> f2s.com (kemlo) Date: Sat Mar 4 07:24:27 2006 Subject: [Histonet] Smears revisited In-Reply-To: <8C80D22F16A2CB3-214-984@FWM-R03.sysops.aol.com> Message-ID: <200603041324.k24DOI5K010945@outmail.freedom2surf.net> Air dried 'pressed' smears rather than fixed 'blood films' or pressed slides. After air drying fix in methanol then stain Romanofski. IMHO -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of bjdewe@aol.com Sent: 03 March 2006 21:46 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Smears revisited So, what is the best way to make smear slides from Lymphoma FNA's? I've seen several methods for different kinds of smears but not sure if they all work for every kind of condition? Lorie ******************************************************************* Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s)and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ******************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GODSGIRLNOW <@t> msn.com Sat Mar 4 08:36:42 2006 From: GODSGIRLNOW <@t> msn.com (Roxanne Soto) Date: Sat Mar 4 08:36:51 2006 Subject: [Histonet] CLIA REGS RE: Grossing: Chime In References: <8B08EC394B366D4A895DD5686D6AFE4A09413E@LP-EXCHVS08.CO.IHC.COM> Message-ID: So in reading the link that you sent me including 493.1489, the person does not have to have a degree, as long as they have 60 credits including all of the sciences and 3 months of training? ----- Original Message ----- From: Lance Erickson To: Roxanne Soto ; Histonet@pathology.swmed.edu Sent: Friday, March 03, 2006 4:10 PM Subject: RE: [Histonet] CLIA REGS RE: Grossing: Chime In www.cms.hhs.gov/clia is the link to the CLIA '88 information. See the new federal register CLIA intrepretive guidelines appendix C subpart M that are effective April 24, 2003. The guidelines are available at www.cms.hhs.gov/CLIA/downloads/apcsubm.pdf under qualifications of high complexity testing personnel section 493.1489 (b)(7) it is clear that all tissue gross examination whether it is color, measurement, or advanced dissection is considered high complexity testing and individuals performing this type of testing must qualify under this section. That is why the new CAP question ANP 11610 was instated and is effective April 28, 2005. CAP must abide by CLIA regulations and CLIA is part of the Center for Medicare and Medicaid Services which is a section of the US government's department of Health and Human Services. So if you would like to maintain your CLIA license and CAP certification and be paid by Medicare you must abide by the requirements for high complexity testing personnel for each person performing any kind of gross examination. The actual wording is: "Interpretive Guidelines ?493.1489(b)(7)In the case of gross examinations, the technical supervisor may delegate to individuals qualified under ?493.1489 the responsibility for the physical examination/description, including color, weight, measurement and other characteristics of the tissue; or other mechanical procedures for which a specific written protocol has been developed. The technical supervisor is ultimately responsible for the diagnosis related to the gross examination and must sign the examination report. The technical supervisor is not required to provide direct onsite supervision but is responsible for the accuracy of all test results reported. All physical examinations/descriptions of tissue including color, weight, measurement and other characteristics of the tissue; or other mechanical procedures performed in the absence of the technical supervisor by individuals qualified under ?493.1489 should be reviewed within 24 hours by the technical supervisor. All microscopic tissue examinations must be performed by individuals qualified under ?493.1449(b), (l) or (m), as appropriate." Lance Erickson Anatomic Pathology Supervisor Primary Children's Medical Center Salt Lake City, UT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roxanne Soto Sent: Friday, March 03, 2006 1:21 PM To: LuckG@empirehealth.org; Charles.Embrey@carle.com; Histonet@pathology.swmed.edu Subject: RE: [Histonet] CLIA REGS RE: Grossing: Chime In Greg, Thank you for your comment-that is exactly what I was trying to ask, but you put it so much better than I did. This is what I need to know, as well, and I have had a very difficult time trying to find the answer by looking at the CLIA website. Roxanne ______________________________________________________________ From: "Luck, Greg D." > To: "'Charles.Embrey'" >, Histonet@pathology.swmed.edu Subject: [Histonet] CLIA REGS RE: Grossing: Chime In Date: Fri, 3 Mar 2006 10:47:45 -0800 >Charles, >In answer to the question below about requirements for "non-pathologists >grossing" you cited the specific text from CLIA '88 (493.1489) which states >the requirements for "analyzing patient samples" but I don't see how that >strictly translates to "grossing" (in particular when we may be talking >about the simple transfer of an entire specimen into tissue cassettes with a >visual description and simple specimen prep and set-up as in reducing the >sizes of the sample to be processed; e.g. bisection with a scalpel. >Wouldn't simple (where the entire specimen is submitted and no independent >decision has to be made over what portions of the specimen to submit and/or >not submit for micro exam by the pathologist be analogous for example to a >Micro lab aid who does this on tissue cultures. "Grossing" by your comment >below does not differentiate between shave biopsy and a prostatectomy. >Where in CLIA does it's 'text' specifically state what constitutes >"grossing" and who can or can not perform CLIA's definition of grossing. >For those of us less familiar with the federal register can you provide >me/us with the specific text from the federal register that you cite in your >response to the 2nd question of "does measuring cores and placing them in a >cassette count as grossing?" to which you have said yes it does. Thanks, >Greg >Greg Luck, BS, HT(ASCP) >Anatomic Pathology Supervisor >Deaconess Medical Center >800 W. 5th Ave >Spokane, WA 99204 >Phone 509.473.7077 >Fax 509.473.7133 >luckg@empirehealth.org >www.deaconessmedicalcenter.org > > > > >-----Original Message----- >From: Charles.Embrey [mailto:Charles.Embrey@carle.com] >Sent: Friday, March 03, 2006 7:10 AM >To: Histonet@pathology.swmed.edu >Subject: FW: [Histonet] CLIA REGS > > > >-----Original Message----- >From: Charles.Embrey >Sent: Friday, March 03, 2006 9:09 AM >To: 'Roxanne Soto' >Subject: RE: [Histonet] CLIA REGS > >CLIA '88 lists the requirements for non-pathologists grossing. Grossing is >considered high-complexity testing even if it's a punch biopsy or a shave. > >CLIA '88 states "On or before April 24 1995 (I) be a high school graduate or >equivalent; and (b) have documentation of training appropriate for the test >performed before analyzing patient specimens"................After that date >it requires an associate degree in a biological or chemical science or >medical laboratory technology -or- qualify as a medical technologist with a >bachelor's degree from an accredited institution -or- earned a bachelor's >degree in a chemical, physical, biologic or clinical laboratory science. > >ref. CLIA '88 493.1489 > >As to your question: "Does measuring cores and putting them in a cassette > Count as grossing?" YES it does. Whether a simple small skin tag or >dissection of an entire colon, the requirements are the same. >It falls under the CLIA High Complexity Testing Personnel Qualifications, >Federal Register VOl. 60, No. 78, April 1995, section >493.1489 > >Also CAP requires a written instruction detailing what specimens may be >grossed with direct vs indirect pathologists' observation. Direct means that >the pathologist literally watches over your shoulder while you gross the >specimen. Indirect means that he is readily available to consult. > >Now as far a Florida is concerned: Florida has one of the most stringent >licensing systems in the US. I fully expect, now that Pathologists' >Assistants are a certified fact, that Florida will look closely at their >licensure and may limit grossing in the state to licensed P.A.s, >Pathologists and residents. At this point it is just a guess but I wouldn't >be surprised to see it happen in the not too distant future. > >Charles Embrey, PA(ASCP) >Carle Clinic >Urbana, IL > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roxanne Soto >Sent: Friday, March 03, 2006 6:39 AM >To: HISTONET@PATHOLOGY.SWMED.EDU >Subject: [Histonet] CLIA REGS > > > Would someone from CLIA (or someone who knows the CLIA regs inside > and out) please contact regarding grossing of tissue-----what is > grossing per se--does measuring cores and putting them in a cassette > count as grossing? What education level doesn one have to have >in > order to do this in the state of Florida? We are in desperate need of > lab aides, but our lab aides have always had BS degrees and they > "gross" out prostate cores....... > Thanks in advance > Roxanne Soto >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kzhong888 <@t> yahoo.com Sat Mar 4 13:04:27 2006 From: kzhong888 <@t> yahoo.com (dfs dsaf) Date: Sat Mar 4 13:04:33 2006 Subject: [Histonet] Need to purchase a IEC CTD-Harris Cryostats Message-ID: <20060304190427.83132.qmail@web52910.mail.yahoo.com> Hi all: I am searching for a very old cyrostat, manufacturer IEC, model CTD-Harris . It comes in a small "ice cream cart" type metal box. If you have one for sale, any condition, with/without microtome, please email me at Kzhong888@yahoo.com. thank you much Kirk Zhong --------------------------------- Yahoo! Mail Bring photos to life! New PhotoMail makes sharing a breeze. From petepath <@t> yahoo.com Sun Mar 5 09:20:03 2006 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Sun Mar 5 09:20:09 2006 Subject: [Histonet] Smears revisited Message-ID: <20060305152003.31754.qmail@web30415.mail.mud.yahoo.com> Hi Lorie, For a lymphoma I express the material on a single slide. I will pick up another slide touch it to the material and then smear that material onto a third slide. If there is more than two slides worth of material, quickly keep picking up material and smearing it onto additional slides. If not, make a final smear on whats left on the first slide. Either fix the smears instantly in 95%etoh and do H&E and/or air dry and do a diff quick. Both fixed and air dried are useful and it is worth making both. Rinse the syringe in RPMI. It is important to learn the right amount of material for each slide. Too much will give poor morphology. A good smear can be made even with very little material but making good smears is a skill that takes practice. It must be done very evenly with little more pressure than it takes to run the slides over each other. One can't press too hard or too soft. If you find a good sample under the microscope, ask if possible for a second pass to put in RPMI for flow. If not, hopefully you syringe rinse will have some material. But do not count on it unless you had recognizable material left in the syringe after your first expression on the slide. If quick review of the slide does not show enough material for diagnosis ask for another pass and start again. If you want to call me I can give you more details on making smears than my poor typing allows. Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com From kemlo <@t> f2s.com Sun Mar 5 11:41:58 2006 From: kemlo <@t> f2s.com (kemlo) Date: Sun Mar 5 11:41:59 2006 Subject: [Histonet] Smears revisited In-Reply-To: <20060305152003.31754.qmail@web30415.mail.mud.yahoo.com> Message-ID: <200603051741.k25Hfq5K011950@outmail.freedom2surf.net> But that's what I said? Anyway, best way of dealing with material from a LN is to express it into Cytyc or SurePath preservation fluid then produce a LBC sample from it using the machine; but you can't do air dried. You can look at it, perform ICC and do almost what ever you want. It's a bit like drowning the dogs, getting a taxidermist to stuff them and you will recognise your old friend right away right away, but it won't fly; sorry stuck in an analogy time warp. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stephen Peters M.D. Sent: 05 March 2006 15:20 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Smears revisited Hi Lorie, For a lymphoma I express the material on a single slide. I will pick up another slide touch it to the material and then smear that material onto a third slide. If there is more than two slides worth of material, quickly keep picking up material and smearing it onto additional slides. If not, make a final smear on whats left on the first slide. Either fix the smears instantly in 95%etoh and do H&E and/or air dry and do a diff quick. Both fixed and air dried are useful and it is worth making both. Rinse the syringe in RPMI. It is important to learn the right amount of material for each slide. Too much will give poor morphology. A good smear can be made even with very little material but making good smears is a skill that takes practice. It must be done very evenly with little more pressure than it takes to run the slides over each other. One can't press too hard or too soft. If you find a good sample under the microscope, ask if possible for a second pass to put in RPMI for flow. If not, hopefully you syringe rinse will have some material. But do not count on it unless you had recognizable material left in the syringe after your first expression on the slide. If quick review of the slide does not show enough material for diagnosis ask for another pass and start again. If you want to call me I can give you more details on making smears than my poor typing allows. Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Mar 5 11:55:10 2006 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Sun Mar 5 11:55:28 2006 Subject: [Histonet] Ki67 on mouse kidney tissue In-Reply-To: <3AD0BD3142459B4E9B12CBEAFF2B89B2027EFC9F@lajamrexm01.amer.pfizer.com> Message-ID: <200603051755.k25HtHGm021338@pro12.abac.com> I would recommend Rabbit Monoclonal Ki67 from Lab Vision, I have used it on mouse tissues with good results. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Trajkovic, Dusko Sent: Friday, March 03, 2006 11:13 AM To: Guillermo Palao; Histonet Subject: RE: [Histonet] Ki67 on mouse kidney tissue I also use a Rabbit Monoclonal Ki-67 from Lab Vision. Works great. On Ventana Discovery XT: DAB Map Kit / CC1 Standard / Primary at 1:800 / GaR (Dako) 1:200. Dusko trajkovic -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Guillermo Palao Sent: Friday, March 03, 2006 9:05 AM To: Histonet Subject: [Histonet] Ki67 on mouse kidney tissue Hello all, Does anybody recommend a particular rabbit anti-Ki67 Ab that works well in mouse kidney? I have seen many different commercial Abs but I would like some first-hand information before buying one. Thanks in advance, Guillermo Palao, MD. Ph.D. Laboratorio de Reumatolog?a Centro de Investigaci?n Hospital 12 de Octubre Avda de C?rdoba s/n Madrid 28041 Spain --------------------------------- LLama Gratis a cualquier PC del Mundo. Llamadas a fijos y m?viles desde 1 c?ntimo por minuto. http://es.voice.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bfarris <@t> bjc.org Sun Mar 5 21:01:44 2006 From: bfarris <@t> bjc.org (Brandi Farris) Date: Sun Mar 5 21:02:15 2006 Subject: [Histonet] Needing information about recycling Message-ID: <440B51B80200002E000051F8@bjcgwimd01.carenet.org> Our histology laboratory is looking at recycler from CBG that processes alcohol, xylene and formalin and we're interested in feedback and answers from users of the system. Are there any fumes with the recycler while it is processing formalin? Can we use the recycled formalin for patient specimens or is it for use only on the processor?Will you please tell us the cost and procedure for buffering? Can you estimate about how much tech time is spent with the system? Your response is appreciated. Thank you, Brandi Farris Boone Hospital Center Columbia, MO 65201 From PKamalavenkatesh <@t> wockhardtin.com Sun Mar 5 22:52:43 2006 From: PKamalavenkatesh <@t> wockhardtin.com (PKamalavenkatesh@wockhardtin.com) Date: Sun Mar 5 22:48:39 2006 Subject: [Histonet] histochemistry- alanine aminotransferase -rat liver Message-ID: Dear all, This is Dr.Kamalavenkatesh from wockhardt reserch center,India. As a part of pre clinical safety evaluation of our NCE'S We want to standardize the methodology for quantitative histochemistry of Alanine aminotransfrease(ALT) activity in rat liver that will aid in direct correlation between histopathological findings to that of histochemical findings.It seems that literature regarding the methodology is scanty.Hence, I want to know from you that any body is doing this technique in their laboratory, if so the protocol involved and their experience with the method. Please let us know. Thanks and regards kamalavenkatesh Please Visit our New Corporate Web Site www.wockhardt.com --------------------- Disclaimer ------------------------------------------------------------------------------ Information transmitted by this E-MAIL is proprietary to Wockhardt Ltd. and/ or its Group Companies and/or,its Customers and is intended for use only by the individual or entity to which it is addressed, and may contain information that is privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. --------------------------------------------------------------------------------------------------------------- From roberta.martindale <@t> ualberta.ca Sun Mar 5 23:31:29 2006 From: roberta.martindale <@t> ualberta.ca (Roberta A. Martindale) Date: Sun Mar 5 23:31:34 2006 Subject: [Histonet] Digital camera usage for teaching microanatomy Message-ID: <20060305223129.zng6icoz3ksck00s@webmail.ualberta.ca> Hello, This is my first post to this list. I teach in a Medical Laboratory Science (MLS) program in Alberta, Canada. As part of my teaching duties I am responsible for the microanatomy portion of the undergraduate MLS program Histology course. Currently in our laboratory sessions, when conducting reviews, I am using a multihead microscope which can only accomodate four students at a time. We do have an older microscope with a digital "live image" camera on it, but the resolution is not great (maybe the monitor?). I was searching through the archives and came across some posts on digital camera setups for histology teaching and was wondering if there were any new developments in this area that the group would be willing to share. This type of upgrade would take time, but I would like to start the research regardless. Thank you for the help. Regards, Roberta Martindale ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Roberta A. Martindale BSc(MLS), MLT, MT(ASCP) Lecturer/Clinical Instructor Laboratory Medicine & Pathology University of Alberta 4B4.07 WMC (780)407-3176 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ From jkiernan <@t> uwo.ca Mon Mar 6 00:28:52 2006 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Mon Mar 6 00:28:05 2006 Subject: [Histonet] histochemistry- alanine aminotransferase -rat liver In-Reply-To: References: Message-ID: <440BD6A4.7070207@uwo.ca> This enzyme is mentioned in a few places in Volume 3 of Stoward & Pearse's Histochemistry (3rd edn, 1991). A general method for aminotransferases is given on p.583. It is also necessary to read the explanatory material about the technique on pp 173-176 and elsewhere. This is a rather involved technique, using two auxiliary enzymes, one of which may not be needed. A Google Scholar search for "alanine aminotransferase" histochem* brings up 405 references. The ones at the top of the heap are papers in J. Histochem. Cytochem. in the 1980s. The full text of these papers (with illustrations) will be available as .pdf files, free for anyone in the world to download, at the journal's web site: http://www.jhc.org Your email to Histonet (cited below) refers to lab safety, standardization and other buzz-words. Are you asking how to do the histochemical method, or how to make safety regulations? If you are a researcher, you need to consult the books and internet resources mentioned above. If you are a safety officer, be assured that aminotransferase histochemistry involves no exposure to hazardous substances. -- ------------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 _______________________________ PKamalavenkatesh@wockhardtin.com wrote: > Dear all, > This is Dr.Kamalavenkatesh from wockhardt reserch > center,India. As a part of pre clinical safety evaluation of our NCE'S We > want to standardize the methodology for quantitative histochemistry of > Alanine aminotransfrease(ALT) activity in rat liver that will aid in direct > correlation between histopathological findings to that of histochemical > findings.It seems that literature regarding the methodology is > scanty.Hence, I want to know from you that any body is doing this technique > in their laboratory, if so the protocol involved and their experience with > the method. Please let us know. > > Thanks and regards > kamalavenkatesh > > > > Please Visit our New Corporate Web Site www.wockhardt.com > --------------------- Disclaimer ------------------------------------------------------------------------------ > Information transmitted by this E-MAIL is proprietary to Wockhardt Ltd. and/ or its Group Companies > and/or,its Customers and is intended for use only by the individual or entity to which it is addressed, > and may contain information that is privileged, confidential or exempt from disclosure under applicable law. > If you are not the intended recipient or it appears that this mail has been forwarded to you without proper > authority, you are notified that any use or dissemination of this information in any manner is strictly > prohibited. In such cases, please delete this mail from your records. > --------------------------------------------------------------------------------------------------------------- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- ------------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan[AT]uwo.ca http://publish.uwo.ca/~jkiernan/ http://instruct.uwo.ca/anatomy/530/index.htm _______________________________ From arvind <@t> nbrc.res.in Mon Mar 6 03:04:33 2006 From: arvind <@t> nbrc.res.in (Arvind) Date: Mon Mar 6 02:59:30 2006 Subject: [Histonet] DAB on CELLS References: Message-ID: <001b01c640fc$fd95d870$aa00a8c0@nbrc.res.in> can any one provide protocol for DAB staining on cells using biotinylated secondary antibody Arvind Singh Pundir National Brain Research Centre Manesar Gurgaon, HARYANA INDIA arvind@nbrc.res.in From hbeard27 <@t> hotmail.com Mon Mar 6 04:23:57 2006 From: hbeard27 <@t> hotmail.com (Helen Beard) Date: Mon Mar 6 04:24:06 2006 Subject: [Histonet] (no subject) Message-ID: From Terry.Marshall <@t> rothgen.nhs.uk Mon Mar 6 06:04:44 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Mon Mar 6 06:04:46 2006 Subject: [Histonet] Revealing lymph nodes Message-ID: Bob, All this time on histonet, I never recall reading the imperative of using Davidson's only as a primary fixative. So, I was not holding my feet right. Now let's see, how can we organise separate fixative for breast and colon specimens? Nah, impossible - forget it. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: rsrichmond@aol.com [mailto:rsrichmond@aol.com] Sent: 03 March 2006 17:23 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Revealing lymph nodes Terry Marshall notes having had no success with revealing lymph nodes by post-fixation in Davidson's fixative. Neither have I. The clearing fixative must be used as the initial fixative. An old method is to pass the formalin-fixed tissue through alcohols into xylene (or cedar oil). I've never used this method, and the one time I've seen it used it didn't work. Once in a while there are no more than three lymph nodes in the axillary contents from a breast cancer. Your surgeon should live with it. Bob Richmond _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Mon Mar 6 07:44:10 2006 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Mon Mar 6 07:44:49 2006 Subject: [Histonet] entering multiple specimens Message-ID: <61FA27BFA9398E4CA9184FF9B0759B08660D4E@nt_exchange.lmhealth.org> A question has come up regarding how we enter our specimens. My lab manager is asking why we don't give each individual specimen a unique pathology specimen number. For example, if we receive a pair of tonsils in 2 separate containers, she doesn't understand why we assign one surgical number and list them as specimen 1 and 2 instead of giving them 2 unique surgical numbers. For something like a colonoscopy with 10 specimens that would be 10 unique surgical numbers and 10 reports. I can't image anyone doing this.... never heard of it being done but then I've only worked in 2 histo labs. I don't see it being done this way but I thought I'd ask. So that's my question. Does anybody do it this way? It would seem to really complicate things and a separate report would have to be issued for each specimen number. I'm sure our GI guys would love getting 14 or 15 separate repors for the same case..... My manager doesn't really understand histo but she tries really hard. She always trys to apply the rules of general lab to us. Her argument is that micro, heme, chemistry, etc. all give separate specimen numbers. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Hospital Newark, Ohio 43055 From rsrichmond <@t> aol.com Mon Mar 6 07:58:19 2006 From: rsrichmond <@t> aol.com (rsrichmond@aol.com) Date: Mon Mar 6 07:58:25 2006 Subject: [Histonet] Re: Revealing lymph nodes In-Reply-To: References: Message-ID: <8C80F3D28D0A5A4-11F8-10BEA@FWM-M15.sysops.aol.com> Terry Marshall writes: >>All this time on histonet, I never recall reading the imperative of using Davidson's only as a primary fixative.So, I was not holding my feet right. Now let's see, how can we organise separate fixative for breast and colon specimens? Nah, impossible - forget it.<< What works with colons: if you receive the whole specimen in formalin on the day of surgery, there won't be enough penetration of the mesenteric fat by the formalin to matter. You can separate the mesenteries, slice them thin, and put them in Davidson's fixative, and fix the colon in formalin. This will work nearly as well for axillary lymph nodes and neck dissections, though these specimens really ought to be received unfixed. The technique is useful for neck dissections, but we don't use Davidson's for axillary lymph nodes, and in brief trials of it I have not found it useful. Remember that Davidson's fixative may not support immunostains. You young folks: Watching us old-timers flounder in public like this, you're seeing just how hard it is to learn histologic technique out of a book! Bob Richmond Gastonia NC From kemlo.rogerson <@t> waht.swest.nhs.uk Mon Mar 6 08:08:41 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Mar 6 08:08:59 2006 Subject: [Histonet] entering multiple specimens Message-ID: I suppose to a non-Histologist it would be bizarre not to number each specimen, but do Blood Science number each tube (EDTA, Citrate, etc) separately. From my experience one Patient, one number suffixed by 1, 2, 3 or a, b, c..... Kemlo Rogerson Pathology Manager (recovering Cytologist) Ext 3311 DD 01934 647057 Mob 07749 754194 'It's our differences that make us similar' This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Tom McNemar [mailto:TMcNemar@lmhealth.org] Sent: Monday, March 06, 2006 1:44 PM To: 'histonet@pathology.swmed.edu' Subject: [Histonet] entering multiple specimens A question has come up regarding how we enter our specimens. My lab manager is asking why we don't give each individual specimen a unique pathology specimen number. For example, if we receive a pair of tonsils in 2 separate containers, she doesn't understand why we assign one surgical number and list them as specimen 1 and 2 instead of giving them 2 unique surgical numbers. For something like a colonoscopy with 10 specimens that would be 10 unique surgical numbers and 10 reports. I can't image anyone doing this.... never heard of it being done but then I've only worked in 2 histo labs. I don't see it being done this way but I thought I'd ask. So that's my question. Does anybody do it this way? It would seem to really complicate things and a separate report would have to be issued for each specimen number. I'm sure our GI guys would love getting 14 or 15 separate repors for the same case..... My manager doesn't really understand histo but she tries really hard. She always trys to apply the rules of general lab to us. Her argument is that micro, heme, chemistry, etc. all give separate specimen numbers. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Hospital Newark, Ohio 43055 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Mon Mar 6 08:36:59 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Mon Mar 6 08:36:58 2006 Subject: [Histonet] entering multiple specimens Message-ID: <83AACDB0810528418AA106F9AE9B7F7E01305A35@sjhaexc02.sjha.org> This would be totally unacceptable to me. I've never heard of it being done this way. j Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 404-851-7831 - fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Tom McNemar Sent: Monday, March 06, 2006 8:44 AM To: 'histonet@pathology.swmed.edu' Subject: [Histonet] entering multiple specimens A question has come up regarding how we enter our specimens. My lab manager is asking why we don't give each individual specimen a unique pathology specimen number. For example, if we receive a pair of tonsils in 2 separate containers, she doesn't understand why we assign one surgical number and list them as specimen 1 and 2 instead of giving them 2 unique surgical numbers. For something like a colonoscopy with 10 specimens that would be 10 unique surgical numbers and 10 reports. I can't image anyone doing this.... never heard of it being done but then I've only worked in 2 histo labs. I don't see it being done this way but I thought I'd ask. So that's my question. Does anybody do it this way? It would seem to really complicate things and a separate report would have to be issued for each specimen number. I'm sure our GI guys would love getting 14 or 15 separate repors for the same case..... My manager doesn't really understand histo but she tries really hard. She always trys to apply the rules of general lab to us. Her argument is that micro, heme, chemistry, etc. all give separate specimen numbers. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Hospital Newark, Ohio 43055 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From gillian.2.brown <@t> gsk.com Mon Mar 6 08:51:30 2006 From: gillian.2.brown <@t> gsk.com (gillian.2.brown@gsk.com) Date: Mon Mar 6 08:52:07 2006 Subject: [Histonet] entering multiple specimens In-Reply-To: <61FA27BFA9398E4CA9184FF9B0759B08660D4E@nt_exchange.lmhealth.org> Message-ID: Tom, they way you do it is a unique number per block (sample), the first part identifies the 'block' to the patient, the second (1-n) denotes that there are multiple samples. You can't however have one without the other hence the uniqueness. Presumably there is a spreadsheet/database that these two part numbers go in (so you could say Block 1=xxxxxx,Block 2=xxxxxxx such as other disciplines will have the cross references someplace other than on the samples themselves (ie barcodes etc). Continuous numbering sends me dyslexic when filing the blocks. Having a number which is common means you can see where one 'case' stops and starts. Gill Target Localisation Target Discovery ri- CEDD. GlaxoSmithKline Medicines Research Centre, Gunnelswood Road, STEVENAGE, Hertfordshire. SG1 2NY tel. +44 (0)1438 764119 fax. +44 (0)1438 764782 email. gillian.2.brown@gsk.com http://iwhfp.gsk.com/targetdiscovery/targetlocalisation/default.htm http://ukdiscovery.gsk.com/histopathology/default.htm "Tom McNemar" Sent by: histonet-bounces@lists.utsouthwestern.edu 06-Mar-2006 13:44 To "'histonet@pathology.swmed.edu'" cc Subject [Histonet] entering multiple specimens A question has come up regarding how we enter our specimens. My lab manager is asking why we don't give each individual specimen a unique pathology specimen number. For example, if we receive a pair of tonsils in 2 separate containers, she doesn't understand why we assign one surgical number and list them as specimen 1 and 2 instead of giving them 2 unique surgical numbers. For something like a colonoscopy with 10 specimens that would be 10 unique surgical numbers and 10 reports. I can't image anyone doing this.... never heard of it being done but then I've only worked in 2 histo labs. I don't see it being done this way but I thought I'd ask. So that's my question. Does anybody do it this way? It would seem to really complicate things and a separate report would have to be issued for each specimen number. I'm sure our GI guys would love getting 14 or 15 separate repors for the same case..... My manager doesn't really understand histo but she tries really hard. She always trys to apply the rules of general lab to us. Her argument is that micro, heme, chemistry, etc. all give separate specimen numbers. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Hospital Newark, Ohio 43055 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lchausse <@t> nmh.org Mon Mar 6 08:52:15 2006 From: Lchausse <@t> nmh.org (Chaussey, Leslie) Date: Mon Mar 6 08:52:40 2006 Subject: [Histonet] entering multiple specimens Message-ID: I have seen the one specimen-one accession number approach in dermatopathology labs only. In all of the routine surgical pathology labs I've worked in, we've accessioned based on the surgical procedure so multiple specimens are parts of one accession number. I have however seen multiple accession numbers used when the patient has had different procedures in the same day... for example, an upper GI procedure and they took multiple specimens. Later in the day, the patient had a lower GI procedure and multiple specimens were received in the lab separately. In those instances, specimens from the upper procedure had a different accession number than those from the lower procedure. ----------------------------------------- This message and any included attachments are intended only for the addressee. The information contained in this message is confidential and may constitute proprietary or non-public information under international, federal, or state laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail. From clcses <@t> gmail.com Mon Mar 6 09:22:33 2006 From: clcses <@t> gmail.com (Carmen Contreras-Sesvold) Date: Mon Mar 6 09:22:41 2006 Subject: [Histonet] re:DAB bluespots Message-ID: <46a3be380603060722k2296b649kb13b8722e3c43d3b@mail.gmail.com> I hav also seen the blue spots, In my cases they were out of focus staining. Hope this helps, Carmen From Terry.Marshall <@t> rothgen.nhs.uk Mon Mar 6 09:33:58 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Mon Mar 6 09:33:57 2006 Subject: [Histonet] re:DAB bluespots Message-ID: Out of focus staining! What a great expression, and the makings of a wonderful excuse - I had blue spots in front of my eyes, and the staining was out of focus:-) Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Carmen Contreras-Sesvold [mailto:clcses@gmail.com] Sent: 06 March 2006 15:23 To: histonetters Subject: [Histonet] re:DAB bluespots I hav also seen the blue spots, In my cases they were out of focus staining. Hope this helps, Carmen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RBARNHART <@t> summithealth.org Mon Mar 6 09:47:35 2006 From: RBARNHART <@t> summithealth.org (Rebecca Barnhart) Date: Mon Mar 6 09:48:09 2006 Subject: [Histonet] entering multiple specimens Message-ID: Giving each sample a unique number is how clinical lab specimens are handled. It is hard to get some of the clinical lab staff to understand one person, one date of service = one specimen number. Our protocol is no matter how many procedures the patient has if it is all done on the same day it is one case number, procedures done on another day then another case number. For example the patient has a colonoscopy in the morning and we do a biopsy run and it is determined the patient needs to go to surgery for a resection, 1 case number with a specimen A and B (or however many specimen were sent). It would be to confusing if you give each specimen a unique number, not only for us but for the doctors. >>> Tom McNemar 3/6/2006 8:44:10 AM >>> A question has come up regarding how we enter our specimens. My lab manager is asking why we don't give each individual specimen a unique pathology specimen number. For example, if we receive a pair of tonsils in 2 separate containers, she doesn't understand why we assign one surgical number and list them as specimen 1 and 2 instead of giving them 2 unique surgical numbers. For something like a colonoscopy with 10 specimens that would be 10 unique surgical numbers and 10 reports. I can't image anyone doing this.... never heard of it being done but then I've only worked in 2 histo labs. I don't see it being done this way but I thought I'd ask. So that's my question. Does anybody do it this way? It would seem to really complicate things and a separate report would have to be issued for each specimen number. I'm sure our GI guys would love getting 14 or 15 separate repors for the same case..... My manager doesn't really understand histo but she tries really hard. She always trys to apply the rules of general lab to us. Her argument is that micro, heme, chemistry, etc. all give separate specimen numbers. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Hospital Newark, Ohio 43055 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From clcses <@t> gmail.com Mon Mar 6 09:51:49 2006 From: clcses <@t> gmail.com (Carmen Contreras-Sesvold) Date: Mon Mar 6 09:51:55 2006 Subject: [Histonet] re: re:DAB blue spots Message-ID: <46a3be380603060751h1a877ffeva6f8cc55659569ec@mail.gmail.com> It was stained tissue that was out of focus. In my case, if I focused up to isolate the blue spots I always found that is was stained tissue. However: I must add that my sections are very thick. Thank you for the correction... I also enjoyed the laugh, ... Carmen From Terry.Marshall <@t> rothgen.nhs.uk Mon Mar 6 09:09:02 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Mon Mar 6 10:06:46 2006 Subject: [Histonet] entering multiple specimens Message-ID: One specimen pot = one histology number here. Nevertheless, it does not follow that each histology number has its own report, they can be and are, amalgamated for the report. It is easier to keep tabs on the workload, costs etc, when you work this way. Nobody mentioned one number *per block", so why this is being criticised is beyond my ken. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Chaussey, Leslie [mailto:Lchausse@nmh.org] Sent: 06 March 2006 14:52 To: Tom McNemar; histonet@pathology.swmed.edu Subject: RE: [Histonet] entering multiple specimens I have seen the one specimen-one accession number approach in dermatopathology labs only. In all of the routine surgical pathology labs I've worked in, we've accessioned based on the surgical procedure so multiple specimens are parts of one accession number. I have however seen multiple accession numbers used when the patient has had different procedures in the same day... for example, an upper GI procedure and they took multiple specimens. Later in the day, the patient had a lower GI procedure and multiple specimens were received in the lab separately. In those instances, specimens from the upper procedure had a different accession number than those from the lower procedure. ----------------------------------------- This message and any included attachments are intended only for the addressee. The information contained in this message is confidential and may constitute proprietary or non-public information under international, federal, or state laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail. From vazquezr <@t> ohsu.edu Mon Mar 6 09:19:37 2006 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Mon Mar 6 10:07:17 2006 Subject: [Histonet] entering multiple specimens Message-ID: Hello, You don't do it that way, for the fact of keeping tract of the number of patients you process. You usually follow it with a 1-2-3 or a-b-c. Robyn OHSU From donna <@t> milestonemed.com Mon Mar 6 09:32:11 2006 From: donna <@t> milestonemed.com (Donna Willis) Date: Mon Mar 6 10:07:32 2006 Subject: [Histonet] Obex Histowraps Message-ID: <1141659142_12114@mail.serverlogistics.com> Can someone in Histoland let me know where I can purchase Obex Histowraps. Thanks, Donna Willis Milestone Medical North American Application Manager From rjbuesa <@t> yahoo.com Mon Mar 6 10:15:50 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 6 10:15:55 2006 Subject: [Histonet] Needing information about recycling In-Reply-To: <440B51B80200002E000051F8@bjcgwimd01.carenet.org> Message-ID: <20060306161550.65594.qmail@web61221.mail.yahoo.com> Hi Brandi: When you recycle alcohol or xylene, you will get alcohol and xylene without the impurities (water, dissolved paraffin, tissue fats) and you will be able to use them again in any of your different protocols (iether processing or ataining). When you recycle used formalin, you will get back formalin in solution but without the buffer salts. This means that you will have to add the salts again, and will be exposed to the hazards of preparing NBF and that is probably why you bought the NBF from a vendor in the first place (at least that was my case). Recycling alcohol takes about twice the time as recycling xylene. For all the above considerations it was why I only recycled xylene, never alcohol and for sure would never even attempt to recycle formalin. Our recycler was paid off in less than 3 years time just by recycling xylene. I hope this will help you. Ren? J. Brandi Farris wrote: Our histology laboratory is looking at recycler from CBG that processes alcohol, xylene and formalin and we're interested in feedback and answers from users of the system. Are there any fumes with the recycler while it is processing formalin? Can we use the recycled formalin for patient specimens or is it for use only on the processor?Will you please tell us the cost and procedure for buffering? Can you estimate about how much tech time is spent with the system? Your response is appreciated. Thank you, Brandi Farris Boone Hospital Center Columbia, MO 65201 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Relax. Yahoo! Mail virus scanning helps detect nasty viruses! From Terry.Marshall <@t> rothgen.nhs.uk Mon Mar 6 10:26:51 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Mon Mar 6 10:27:02 2006 Subject: [Histonet] Smears revisited Message-ID: "One can't press too hard or too soft." This is the sort of expression that only native English speakers understand. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Stephen Peters M.D. [mailto:petepath@yahoo.com] Sent: 05 March 2006 15:20 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Smears revisited Hi Lorie, For a lymphoma I express the material on a single slide. I will pick up another slide touch it to the material and then smear that material onto a third slide. If there is more than two slides worth of material, quickly keep picking up material and smearing it onto additional slides. If not, make a final smear on whats left on the first slide. Either fix the smears instantly in 95%etoh and do H&E and/or air dry and do a diff quick. Both fixed and air dried are useful and it is worth making both. Rinse the syringe in RPMI. It is important to learn the right amount of material for each slide. Too much will give poor morphology. A good smear can be made even with very little material but making good smears is a skill that takes practice. It must be done very evenly with little more pressure than it takes to run the slides over each other. One can't press too hard or too soft. If you find a good sample under the microscope, ask if possible for a second pass to put in RPMI for flow. If not, hopefully you syringe rinse will have some material. But do not count on it unless you had recognizable material left in the syringe after your first expression on the slide. If quick review of the slide does not show enough material for diagnosis ask for another pass and start again. If you want to call me I can give you more details on making smears than my poor typing allows. Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Mon Mar 6 10:29:23 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Mon Mar 6 10:29:21 2006 Subject: [Histonet] Smears revisited Message-ID: In the far off days when I used to make films, I found X-ray film better than using another slide. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Stephen Peters M.D. [mailto:petepath@yahoo.com] Sent: 05 March 2006 15:20 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Smears revisited Hi Lorie, For a lymphoma I express the material on a single slide. I will pick up another slide touch it to the material and then smear that material onto a third slide. If there is more than two slides worth of material, quickly keep picking up material and smearing it onto additional slides. If not, make a final smear on whats left on the first slide. Either fix the smears instantly in 95%etoh and do H&E and/or air dry and do a diff quick. Both fixed and air dried are useful and it is worth making both. Rinse the syringe in RPMI. It is important to learn the right amount of material for each slide. Too much will give poor morphology. A good smear can be made even with very little material but making good smears is a skill that takes practice. It must be done very evenly with little more pressure than it takes to run the slides over each other. One can't press too hard or too soft. If you find a good sample under the microscope, ask if possible for a second pass to put in RPMI for flow. If not, hopefully you syringe rinse will have some material. But do not count on it unless you had recognizable material left in the syringe after your first expression on the slide. If quick review of the slide does not show enough material for diagnosis ask for another pass and start again. If you want to call me I can give you more details on making smears than my poor typing allows. Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo.rogerson <@t> waht.swest.nhs.uk Mon Mar 6 10:39:04 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Mar 6 10:39:26 2006 Subject: [Histonet] Smears revisited Message-ID: Unless it's a place.... Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: Monday, March 06, 2006 4:27 PM To: Stephen Peters M.D.; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Smears revisited "One can't press too hard or too soft." This is the sort of expression that only native English speakers understand. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Stephen Peters M.D. [mailto:petepath@yahoo.com] Sent: 05 March 2006 15:20 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Smears revisited Hi Lorie, For a lymphoma I express the material on a single slide. I will pick up another slide touch it to the material and then smear that material onto a third slide. If there is more than two slides worth of material, quickly keep picking up material and smearing it onto additional slides. If not, make a final smear on whats left on the first slide. Either fix the smears instantly in 95%etoh and do H&E and/or air dry and do a diff quick. Both fixed and air dried are useful and it is worth making both. Rinse the syringe in RPMI. It is important to learn the right amount of material for each slide. Too much will give poor morphology. A good smear can be made even with very little material but making good smears is a skill that takes practice. It must be done very evenly with little more pressure than it takes to run the slides over each other. One can't press too hard or too soft. If you find a good sample under the microscope, ask if possible for a second pass to put in RPMI for flow. If not, hopefully you syringe rinse will have some material. But do not count on it unless you had recognizable material left in the syringe after your first expression on the slide. If quick review of the slide does not show enough material for diagnosis ask for another pass and start again. If you want to call me I can give you more details on making smears than my poor typing allows. Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From froyer <@t> bitstream.net Mon Mar 6 10:41:31 2006 From: froyer <@t> bitstream.net (Ford Royer) Date: Mon Mar 6 10:41:45 2006 Subject: [Histonet] entering multiple specimens In-Reply-To: Message-ID: <000f01c6413c$d30967d0$6f01a80a@fords> Having been in both the clinical and anatomical sides of the lab maybe this will help you explain it to your lab manager. (Then again, maybe not) Rebecca hit the confusion point correctly. If your lab manager is accustom to the way the clinical lab specimens are numbered then she is probably use to assigning a different number to specimens that go to different departments within the clinical lab. Example: If a battery of tests were ordered on a patient, such as a Chemistry Profile, a Complete Blood Count (CBC), and a Blood Culture... three (3) separate whole blood specimens would be collected from the same patient, at the same time, on the same day. One specimen (in a red top tube) would be assigned a number and sent to the Chem dept.; another specimen (in a lavender top tube) would be given a separate number and sent to the Hemo dept.; and a third specimen (in a sterile blood culture bottle) would be given yet another number and sent to the Micro dept. However... and this is where you may help your manager in understanding how surgical specimens are numbered differently... the tube that was sent to Chemistry had a 12-parameter chemistry profile done on it. (Glucose, Triglycerides, Cholesterol, BUN, GGT, Total Protein, etc., etc.) Should the Chemistry department then give each individual parameter a separate ID number? ... The specimen that went to Hematology had a CBC and a Differential performed. Should there be two ID numbers. One for the blood tube and one for the slide smear that was stained for the differential. Let your manager think about this one. Now in the case of Tonsils, you have one procedure, one patient, two specimens. Same I.D. number with separate designations of "A" and "B" (or "1" and "2", "Left" and "Right" etc.) Make sense? (I hope so... I'm not sure if I understand it myself) ;-) ~ Ford Ford M. Royer, MT(ASCP) Minnesota Medical Specialists Minneapolis, MN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rebecca Barnhart Sent: Monday, March 06, 2006 9:48 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] entering multiple specimens Giving each sample a unique number is how clinical lab specimens are handled. It is hard to get some of the clinical lab staff to understand one person, one date of service = one specimen number. Our protocol is no matter how many procedures the patient has if it is all done on the same day it is one case number, procedures done on another day then another case number. For example the patient has a colonoscopy in the morning and we do a biopsy run and it is determined the patient needs to go to surgery for a resection, 1 case number with a specimen A and B (or however many specimen were sent). It would be to confusing if you give each specimen a unique number, not only for us but for the doctors. >>> Tom McNemar 3/6/2006 8:44:10 AM >>> A question has come up regarding how we enter our specimens. My lab manager is asking why we don't give each individual specimen a unique pathology specimen number. For example, if we receive a pair of tonsils in 2 separate containers, she doesn't understand why we assign one surgical number and list them as specimen 1 and 2 instead of giving them 2 unique surgical numbers. For something like a colonoscopy with 10 specimens that would be 10 unique surgical numbers and 10 reports. I can't image anyone doing this.... never heard of it being done but then I've only worked in 2 histo labs. I don't see it being done this way but I thought I'd ask. So that's my question. Does anybody do it this way? It would seem to really complicate things and a separate report would have to be issued for each specimen number. I'm sure our GI guys would love getting 14 or 15 separate repors for the same case..... My manager doesn't really understand histo but she tries really hard. She always trys to apply the rules of general lab to us. Her argument is that micro, heme, chemistry, etc. all give separate specimen numbers. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Hospital Newark, Ohio 43055 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Mon Mar 6 10:45:26 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon Mar 6 10:45:23 2006 Subject: AW: [Histonet] entering multiple specimens In-Reply-To: Message-ID: <000901c6413d$5f1e0050$eeeea8c0@SERVER01> We do it also in this way. One pot is one number. One patient can have several numbers that are joined together by the laboratory system. There are patient-ID-numbers and case-numbers, that are created by the hospital system. The patient-ID identifies the person for his/her life, the case-number refers to all procedures done with the patient while his/her stay in the hospital. We identify the single block with extra letters (a,b,c,..) and "codes". In this country it is a regular way of organization. Gudrun Lang Akh Linz Austria -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Marshall Terry Dr,Consultant Histopathologist Gesendet: Montag, 06. M?rz 2006 16:09 An: Chaussey, Leslie; Tom McNemar; histonet@pathology.swmed.edu Betreff: RE: [Histonet] entering multiple specimens One specimen pot = one histology number here. Nevertheless, it does not follow that each histology number has its own report, they can be and are, amalgamated for the report. It is easier to keep tabs on the workload, costs etc, when you work this way. Nobody mentioned one number *per block", so why this is being criticised is beyond my ken. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Chaussey, Leslie [mailto:Lchausse@nmh.org] Sent: 06 March 2006 14:52 To: Tom McNemar; histonet@pathology.swmed.edu Subject: RE: [Histonet] entering multiple specimens I have seen the one specimen-one accession number approach in dermatopathology labs only. In all of the routine surgical pathology labs I've worked in, we've accessioned based on the surgical procedure so multiple specimens are parts of one accession number. I have however seen multiple accession numbers used when the patient has had different procedures in the same day... for example, an upper GI procedure and they took multiple specimens. Later in the day, the patient had a lower GI procedure and multiple specimens were received in the lab separately. In those instances, specimens from the upper procedure had a different accession number than those from the lower procedure. ----------------------------------------- This message and any included attachments are intended only for the addressee. The information contained in this message is confidential and may constitute proprietary or non-public information under international, federal, or state laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JurciseJ <@t> pediatrics.ohio-state.edu Mon Mar 6 10:48:44 2006 From: JurciseJ <@t> pediatrics.ohio-state.edu (Jurcisek, Joseph) Date: Mon Mar 6 10:49:43 2006 Subject: [Histonet] Digital camera usage for teaching microanatomy Message-ID: <714B9F12B4E18C4C843B66E8E190F2ADB1ED32@res2k3ms01.CRII.ORG> Zeiss has some nice systems that are user friendly and do not cost too much. I use the AxioCam and switch the camera between my dissecting scope, brightfield, and fluorescent. I also use it for teaching not only microanatomy but dissection techniques. Joe Joseph A. Jurcisek Senior Research Associate Center for Microbial Pathogenesis Columbus Children's Research Institute 700 Children's Dr. Columbus, OH 43205-2696 (614) 355-3521 fax (614) 722-2818 jurcisej@ccri.net -----Original Message----- From: Roberta A. Martindale [mailto:roberta.martindale@ualberta.ca] Sent: Monday, March 06, 2006 12:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Digital camera usage for teaching microanatomy Hello, This is my first post to this list. I teach in a Medical Laboratory Science (MLS) program in Alberta, Canada. As part of my teaching duties I am responsible for the microanatomy portion of the undergraduate MLS program Histology course. Currently in our laboratory sessions, when conducting reviews, I am using a multihead microscope which can only accomodate four students at a time. We do have an older microscope with a digital "live image" camera on it, but the resolution is not great (maybe the monitor?). I was searching through the archives and came across some posts on digital camera setups for histology teaching and was wondering if there were any new developments in this area that the group would be willing to share. This type of upgrade would take time, but I would like to start the research regardless. Thank you for the help. Regards, Roberta Martindale ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Roberta A. Martindale BSc(MLS), MLT, MT(ASCP) Lecturer/Clinical Instructor Laboratory Medicine & Pathology University of Alberta 4B4.07 WMC (780)407-3176 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ From Terry.Marshall <@t> rothgen.nhs.uk Mon Mar 6 10:11:44 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Mon Mar 6 11:08:34 2006 Subject: [Histonet] entering multiple specimens Message-ID: It would help if you just gave a teeny weenie hint as to what you are talking about. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Robyn Vazquez [mailto:vazquezr@ohsu.edu] Sent: 06 March 2006 15:20 To: TMcNemar@lmhealth.org; histonet@pathology.swmed.edu; JWEEMS@sjha.org Subject: RE: [Histonet] entering multiple specimens Hello, You don't do it that way, for the fact of keeping tract of the number of patients you process. You usually follow it with a 1-2-3 or a-b-c. Robyn OHSU _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJasper <@t> smdc.org Mon Mar 6 10:35:20 2006 From: TJasper <@t> smdc.org (Jasper, Thomas G.) Date: Mon Mar 6 11:08:44 2006 Subject: [Histonet] entering multiple specimens Message-ID: <1A9F2A6C5762524799A816F1F09744CF1E09C5@SCREECH.ntcampus.smdc.org> Tom, I concur with the responses you've already received to your posting. I also think it's good that your manager is trying to understand histology which can be half the battle. When surgical numbers are assigned the accession number is a "case" number as well. Perhaps if you could get her to look at from a case perspective she'd grasp the concept more easily. I'm sure you receive what we call "gross only" cases. There are no blocks generated and our pathologist or PA will dictate a gross description (varicose vein for example). We do hold these specimens (like others, if there's remaining tissue) but that's it, however it's still a case. I know you know this but maybe your manager doesn't. So, gross only, is a separate case. Multiple specimens from the same patient during the same procedure, is a separate case. Multiple specimens from the same patient during the same procedure are not multiple cases. Again, I know you know this, I'm just trying to help you with your manager. Also if she could take a look at some path reports, maybe this whole case stuff would come clear to her. Can you imagine a pathologist putting together a report with multiple specimens and commenting on each specimen as another case number? In a multiple specimen case, the specimens tie the case together. Maybe she could see the logic of that? I've experienced, what I call, the "yoke of clinical lab" trying to be put on us in pathology. And you're right, a lot of these folks don't understand. Clinical lab doesn't always fit pathology. I could go on but I won't. I hope this helps and good luck to you. Thomas Jasper HT (ASCP) BAS AP Supervisor SMDC-Duluth, MN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Tom McNemar Sent: Monday, March 06, 2006 7:44 AM To: 'histonet@pathology.swmed.edu' Subject: [Histonet] entering multiple specimens A question has come up regarding how we enter our specimens. My lab manager is asking why we don't give each individual specimen a unique pathology specimen number. For example, if we receive a pair of tonsils in 2 separate containers, she doesn't understand why we assign one surgical number and list them as specimen 1 and 2 instead of giving them 2 unique surgical numbers. For something like a colonoscopy with 10 specimens that would be 10 unique surgical numbers and 10 reports. I can't image anyone doing this.... never heard of it being done but then I've only worked in 2 histo labs. I don't see it being done this way but I thought I'd ask. So that's my question. Does anybody do it this way? It would seem to really complicate things and a separate report would have to be issued for each specimen number. I'm sure our GI guys would love getting 14 or 15 separate repors for the same case..... My manager doesn't really understand histo but she tries really hard. She always trys to apply the rules of general lab to us. Her argument is that micro, heme, chemistry, etc. all give separate specimen numbers. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Hospital Newark, Ohio 43055 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From tpmorken <@t> labvision.com Mon Mar 6 10:37:24 2006 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Mon Mar 6 11:08:49 2006 Subject: [Histonet] entering multiple specimens Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA20328D6D2@usca0082k08.labvision.apogent.com> Tom, the common pathology numbering schemes I have seen do give each specimen a unique number, though all use sub-numbers/codes to the original accession number. So a number like S05-1234-1-A is "Surgical 2005, Accession number 1234, part 1, block A. This uniquely identifies the tissue part and even the block, so is much more preferable to separate numbers for each specimen because the specimens are generally from a single PROCEDURE, so are logically grouped together. If we were to give separate accession numbers to differnet parts of tissue taken at the same time, to then later try to group them somehow would require subcodes, just as is done now. So there would be no practical advantage. Your lab manager is most likely a med tech, and they usually work with discreet specimens such as single blood draws, etc, that do not need to be related by anything other than they are from the same person. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Monday, March 06, 2006 5:44 AM To: 'histonet@pathology.swmed.edu' Subject: [Histonet] entering multiple specimens A question has come up regarding how we enter our specimens. My lab manager is asking why we don't give each individual specimen a unique pathology specimen number. For example, if we receive a pair of tonsils in 2 separate containers, she doesn't understand why we assign one surgical number and list them as specimen 1 and 2 instead of giving them 2 unique surgical numbers. For something like a colonoscopy with 10 specimens that would be 10 unique surgical numbers and 10 reports. I can't image anyone doing this.... never heard of it being done but then I've only worked in 2 histo labs. I don't see it being done this way but I thought I'd ask. So that's my question. Does anybody do it this way? It would seem to really complicate things and a separate report would have to be issued for each specimen number. I'm sure our GI guys would love getting 14 or 15 separate repors for the same case..... My manager doesn't really understand histo but she tries really hard. She always trys to apply the rules of general lab to us. Her argument is that micro, heme, chemistry, etc. all give separate specimen numbers. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Hospital Newark, Ohio 43055 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Mon Mar 6 12:00:53 2006 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Mon Mar 6 12:01:20 2006 Subject: [Histonet] entering multiple specimens Message-ID: <61FA27BFA9398E4CA9184FF9B0759B08660D4F@nt_exchange.lmhealth.org> I used the example of a chemistry profile with multiple test run and her reply was that the entire profile done from a single specimens. Just a little clarification... we do give each specimen a unique number each case gets one accession number and multiple specimens within that case get identified as #1, #2, etc. Thanks so much for all the comments..... Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Hospital Newark, Ohio 43055 -----Original Message----- From: Ford Royer [mailto:froyer@bitstream.net] Sent: Monday, March 06, 2006 11:42 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] entering multiple specimens Having been in both the clinical and anatomical sides of the lab maybe this will help you explain it to your lab manager. (Then again, maybe not) Rebecca hit the confusion point correctly. If your lab manager is accustom to the way the clinical lab specimens are numbered then she is probably use to assigning a different number to specimens that go to different departments within the clinical lab. Example: If a battery of tests were ordered on a patient, such as a Chemistry Profile, a Complete Blood Count (CBC), and a Blood Culture... three (3) separate whole blood specimens would be collected from the same patient, at the same time, on the same day. One specimen (in a red top tube) would be assigned a number and sent to the Chem dept.; another specimen (in a lavender top tube) would be given a separate number and sent to the Hemo dept.; and a third specimen (in a sterile blood culture bottle) would be given yet another number and sent to the Micro dept. However... and this is where you may help your manager in understanding how surgical specimens are numbered differently... the tube that was sent to Chemistry had a 12-parameter chemistry profile done on it. (Glucose, Triglycerides, Cholesterol, BUN, GGT, Total Protein, etc., etc.) Should the Chemistry department then give each individual parameter a separate ID number? ... The specimen that went to Hematology had a CBC and a Differential performed. Should there be two ID numbers. One for the blood tube and one for the slide smear that was stained for the differential. Let your manager think about this one. Now in the case of Tonsils, you have one procedure, one patient, two specimens. Same I.D. number with separate designations of "A" and "B" (or "1" and "2", "Left" and "Right" etc.) Make sense? (I hope so... I'm not sure if I understand it myself) ;-) ~ Ford Ford M. Royer, MT(ASCP) Minnesota Medical Specialists Minneapolis, MN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rebecca Barnhart Sent: Monday, March 06, 2006 9:48 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] entering multiple specimens Giving each sample a unique number is how clinical lab specimens are handled. It is hard to get some of the clinical lab staff to understand one person, one date of service = one specimen number. Our protocol is no matter how many procedures the patient has if it is all done on the same day it is one case number, procedures done on another day then another case number. For example the patient has a colonoscopy in the morning and we do a biopsy run and it is determined the patient needs to go to surgery for a resection, 1 case number with a specimen A and B (or however many specimen were sent). It would be to confusing if you give each specimen a unique number, not only for us but for the doctors. >>> Tom McNemar 3/6/2006 8:44:10 AM >>> A question has come up regarding how we enter our specimens. My lab manager is asking why we don't give each individual specimen a unique pathology specimen number. For example, if we receive a pair of tonsils in 2 separate containers, she doesn't understand why we assign one surgical number and list them as specimen 1 and 2 instead of giving them 2 unique surgical numbers. For something like a colonoscopy with 10 specimens that would be 10 unique surgical numbers and 10 reports. I can't image anyone doing this.... never heard of it being done but then I've only worked in 2 histo labs. I don't see it being done this way but I thought I'd ask. So that's my question. Does anybody do it this way? It would seem to really complicate things and a separate report would have to be issued for each specimen number. I'm sure our GI guys would love getting 14 or 15 separate repors for the same case..... My manager doesn't really understand histo but she tries really hard. She always trys to apply the rules of general lab to us. Her argument is that micro, heme, chemistry, etc. all give separate specimen numbers. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Hospital Newark, Ohio 43055 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kathy.Johnston <@t> CLS.ab.ca Mon Mar 6 12:25:09 2006 From: Kathy.Johnston <@t> CLS.ab.ca (Kathy.Johnston@CLS.ab.ca) Date: Mon Mar 6 13:11:03 2006 Subject: [Histonet] M Trichrome on Plastic Embedded Kidneys Message-ID: <4BC300747AF87A48BCDF8E48BC2885CE013652D2@mail1.calgary.com> Does anyone out there do Masson Trichromes staining on Plastic embedded kidney sections? They don't seem to stain too badly with the M Trichrome that we use for our Paraffin sections, but I can't help wondering if there is a specific method that deals with the plastic residue surrounding (and possibly obscuring) the tissue. This is something new we're trying, so any info would be gratefully received! Thanks Kathy CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify the sender immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. From KReif <@t> stegh.on.ca Mon Mar 6 13:30:59 2006 From: KReif <@t> stegh.on.ca (Kevin Reif) Date: Mon Mar 6 13:31:12 2006 Subject: [Histonet] Hi All: Does anyone have information regarding 24 hourfixation and its benefits, if any, for infect Message-ID: <440C47A3020000B600000034@GW_STEGH.int.stegh.on.ca> Hi All: Does anyone have information regarding 24 hour fixation and its benefits, if any, for infection control. Thanks for all input Kevin STEGH footer "Working together to become the Best Community Hospital in Ontario" Confidentiality/Privacy Notice: This information is directed in confidence solely to the person named above and may contain confidential and/or privileged material. This information may not otherwise be distributed, copied or disclosed. If you have received this e-mail in error, please notify the sender immediately via a return e-mail and destroy original message. Thank you for your cooperation. For more information on our Privacy Policy, please see our website at www.stegh.on.ca http://www.stegh.on.ca/ From pruegg <@t> ihctech.net Mon Mar 6 13:29:45 2006 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Mar 6 14:12:36 2006 Subject: [Histonet] M Trichrome on Plastic Embedded Kidneys In-Reply-To: <4BC300747AF87A48BCDF8E48BC2885CE013652D2@mail1.calgary.com> Message-ID: <200603061929.k26JTgU2022826@chip.viawest.net> Kathy, I have a TC protocol developed just for GMA (I assume you are talking GMA which does not get removed). Let me know if you want this protocol. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 216 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy.Johnston@CLS.ab.ca Sent: Monday, March 06, 2006 11:25 AM To: histonet@pathology.swmed.edu Subject: [Histonet] M Trichrome on Plastic Embedded Kidneys Does anyone out there do Masson Trichromes staining on Plastic embedded kidney sections? They don't seem to stain too badly with the M Trichrome that we use for our Paraffin sections, but I can't help wondering if there is a specific method that deals with the plastic residue surrounding (and possibly obscuring) the tissue. This is something new we're trying, so any info would be gratefully received! Thanks Kathy CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify the sender immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MVaughan4 <@t> ucok.edu Mon Mar 6 14:28:57 2006 From: MVaughan4 <@t> ucok.edu (MVaughan4@ucok.edu) Date: Mon Mar 6 14:29:32 2006 Subject: [Histonet] Speakers of native English In-Reply-To: Message-ID: As opposed to those speakers of non-native English! :) Mel Melville B. Vaughan, Ph. D. Assistant Professor Department of Biology University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 Date: Mon, 6 Mar 2006 16:26:51 -0000 From: "Marshall Terry Dr, Consultant Histopathologist" "One can't press too hard or too soft." This is the sort of expression that only native English speakers understand. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk From akbitting <@t> geisinger.edu Mon Mar 6 15:08:19 2006 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Mon Mar 6 15:09:03 2006 Subject: [Histonet] Reproductive Toxins Message-ID: Does anyone know how to tell if something in the Histo lab is a reproductive toxin or acute toxin? Some of the MSDS sheets are no help at all. Anyone else having this problem? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From gpbnas <@t> yahoo.es Mon Mar 6 15:14:48 2006 From: gpbnas <@t> yahoo.es (Guillermo Palao) Date: Mon Mar 6 15:14:54 2006 Subject: [Histonet] Ki67 on mouse kidney tissue In-Reply-To: <200603051755.k25HtHGm021338@pro12.abac.com> Message-ID: <20060306211448.69897.qmail@web26208.mail.ukl.yahoo.com> Thanks a lot to everybody, it seems Lab Vision?s Ab is the most used. According to manufacturer it is optimized to work in paraphin-embedded samples. Does anybody have experience with frozen sections? Thank you all for your useful tips! Guillermo pruegg@ihctech.net escribi?: I would recommend Rabbit Monoclonal Ki67 from Lab Vision, I have used it on mouse tissues with good results. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Trajkovic, Dusko Sent: Friday, March 03, 2006 11:13 AM To: Guillermo Palao; Histonet Subject: RE: [Histonet] Ki67 on mouse kidney tissue I also use a Rabbit Monoclonal Ki-67 from Lab Vision. Works great. On Ventana Discovery XT: DAB Map Kit / CC1 Standard / Primary at 1:800 / GaR (Dako) 1:200. Dusko trajkovic -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Guillermo Palao Sent: Friday, March 03, 2006 9:05 AM To: Histonet Subject: [Histonet] Ki67 on mouse kidney tissue Hello all, Does anybody recommend a particular rabbit anti-Ki67 Ab that works well in mouse kidney? I have seen many different commercial Abs but I would like some first-hand information before buying one. Thanks in advance, Guillermo Palao, MD. Ph.D. Laboratorio de Reumatolog?a Centro de Investigaci?n Hospital 12 de Octubre Avda de C?rdoba s/n Madrid 28041 Spain --------------------------------- LLama Gratis a cualquier PC del Mundo. Llamadas a fijos y m?viles desde 1 c?ntimo por minuto. http://es.voice.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- LLama Gratis a cualquier PC del Mundo. Llamadas a fijos y m?viles desde 1 c?ntimo por minuto. http://es.voice.yahoo.com From b003046 <@t> nf.au.dk Mon Mar 6 15:56:13 2006 From: b003046 <@t> nf.au.dk (b003046@nf.au.dk) Date: Mon Mar 6 15:56:21 2006 Subject: [Histonet] CD34/CD45 Message-ID: <1141682173.440caffda65c4@webmail.nf.au.dk> Does anyone knows a good CD34- and CD45 antibody that works on rat tissue? Any information would be greatly appreciated. kind regards Mette K. Hagensen Department of Cardiology B, Research Unit Aarhus University Hospital, Skejby Hospital Brendstrupgaardsvej 100 8200 Aarhus N Denmark Mail: b003046@nf.au.dk From biswas.16 <@t> osu.edu Mon Mar 6 16:15:53 2006 From: biswas.16 <@t> osu.edu (SABYASACHI BISWAS) Date: Mon Mar 6 16:15:59 2006 Subject: [Histonet] Diabetic IHC Message-ID: <2fde80f2fe1aef.2fe1aef2fde80f@osu.edu> Hi Everyone We are planning to look for diabetes markersin diabetic mice as well as in human biopsies. Carboxymethyl lysine is our main target of interest but ofcourse any advanced glycated endproduct will do. Are there any commercial antibodies to carboxymethyl lysine available? or to any AGE? Most references I've found have generated their own antibody. We would also be interested in any hybridomas for theese antibodies. Also for the record we will work primarily on formalin fixed paraffin embedded skin biopsies but can also use frozen samples if we absolutely have to. TIA for any help and suggestions. Sabya Biswas The Ohio State University From jkiernan <@t> uwo.ca Mon Mar 6 16:26:22 2006 From: jkiernan <@t> uwo.ca (John A. Kiernan) Date: Mon Mar 6 16:36:20 2006 Subject: [Histonet] Reproductive Toxins References: Message-ID: <440CB70E.DFAA307C@uwo.ca> Toxins are substances produced by certain living organisms (bacteria, fungi, snakes etc) or released from dead ones (eg endotoxins). I don't think any toxins are used in ordinary histology labs. Fluorescently labelled alpha-bungarotoxin, from a S.E.Asian krait, can be used to stain motor endplates by binding to the nicotinic acetylcholine receptor. This is a decidedly "acute" neurotoxin, but the paralysis it causes would also inhibit reproductive activities. John Kiernan Anatomy, UWO London, Canada. ----------------------------------- Angela Bitting wrote: > > Does anyone know how to tell if something in the Histo lab is a reproductive toxin or acute toxin? Some of the MSDS sheets are no help at all. Anyone else having this problem? > > Angela Bitting, HT(ASCP) > Technical Specialist, Histology > Geisinger Medical Center > 100 N Academy Ave. MC 23-00 > Danville, PA 17822 > phone 570-214-9634 > fax 570-271-5916 > > IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo.rogerson <@t> waht.swest.nhs.uk Tue Mar 7 02:16:32 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Mar 7 02:16:46 2006 Subject: [Histonet] Speakers of native English Message-ID: If Terry had said a Native (noun) of England rather than native (adj) English it may have worked but that's semantics; we know what he meant. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 "Following the line of least resistance makes both men and rivers crooked" This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: MVaughan4@ucok.edu [mailto:MVaughan4@ucok.edu] Sent: Monday, March 06, 2006 8:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Speakers of native English As opposed to those speakers of non-native English! :) Mel Melville B. Vaughan, Ph. D. Assistant Professor Department of Biology University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 Date: Mon, 6 Mar 2006 16:26:51 -0000 From: "Marshall Terry Dr, Consultant Histopathologist" "One can't press too hard or too soft." This is the sort of expression that only native English speakers understand. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo.rogerson <@t> waht.swest.nhs.uk Tue Mar 7 02:30:29 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Mar 7 02:30:43 2006 Subject: [Histonet] entering multiple specimens Message-ID: Makes a bugger of trying to compare workloads though. Those that insist on counting everything including 'next door's cat' seem to have larger work loads than those who number logically. ?frustra esse? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Gudrun Lang [mailto:gu.lang@gmx.at] Sent: Monday, March 06, 2006 4:45 PM To: Histonetliste (Histonetliste) Subject: AW: [Histonet] entering multiple specimens We do it also in this way. One pot is one number. One patient can have several numbers that are joined together by the laboratory system. There are patient-ID-numbers and case-numbers, that are created by the hospital system. The patient-ID identifies the person for his/her life, the case-number refers to all procedures done with the patient while his/her stay in the hospital. We identify the single block with extra letters (a,b,c,..) and "codes". In this country it is a regular way of organization. Gudrun Lang Akh Linz Austria -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Marshall Terry Dr,Consultant Histopathologist Gesendet: Montag, 06. M?rz 2006 16:09 An: Chaussey, Leslie; Tom McNemar; histonet@pathology.swmed.edu Betreff: RE: [Histonet] entering multiple specimens One specimen pot = one histology number here. Nevertheless, it does not follow that each histology number has its own report, they can be and are, amalgamated for the report. It is easier to keep tabs on the workload, costs etc, when you work this way. Nobody mentioned one number *per block", so why this is being criticised is beyond my ken. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Chaussey, Leslie [mailto:Lchausse@nmh.org] Sent: 06 March 2006 14:52 To: Tom McNemar; histonet@pathology.swmed.edu Subject: RE: [Histonet] entering multiple specimens I have seen the one specimen-one accession number approach in dermatopathology labs only. In all of the routine surgical pathology labs I've worked in, we've accessioned based on the surgical procedure so multiple specimens are parts of one accession number. I have however seen multiple accession numbers used when the patient has had different procedures in the same day... for example, an upper GI procedure and they took multiple specimens. Later in the day, the patient had a lower GI procedure and multiple specimens were received in the lab separately. In those instances, specimens from the upper procedure had a different accession number than those from the lower procedure. ----------------------------------------- This message and any included attachments are intended only for the addressee. The information contained in this message is confidential and may constitute proprietary or non-public information under international, federal, or state laws. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hbeard27 <@t> hotmail.com Tue Mar 7 04:50:24 2006 From: hbeard27 <@t> hotmail.com (Helen Beard) Date: Tue Mar 7 04:50:31 2006 Subject: [Histonet] frozen sections Message-ID: I have been given some stored mouse brains (-70C), that were fixed by paraformaldehyde perfusion, cryosectioned, wrapped in foil and stored for about a year. A couple of attempts at IHC on previously cut and stored frozen sections and freshly cut sections have resulted in the sections floating off at various stages. The blocks were difficult to cut (?tissue dried out) with the sections being picked up on silanised slides. I tried air drying for 1 hour & air dry/cold acetone/air drying prior to IHC. Should I air dry for an extended period, try super frost plus slides or is the fact that the tissue may have dried out causing most of my difficulties? Any suggestions as to how I can prevent the sections floating off during IHC would be greatly appreciated. Helen From Terry.Marshall <@t> rothgen.nhs.uk Tue Mar 7 05:32:34 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Tue Mar 7 05:32:37 2006 Subject: [Histonet] Speakers of native English Message-ID: Um - well - err - yes (ish). Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:kemlo.rogerson@waht.swest.nhs.uk] Sent: 07 March 2006 08:17 To: 'MVaughan4@ucok.edu'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Speakers of native English If Terry had said a Native (noun) of England rather than native (adj) English it may have worked but that's semantics; we know what he meant. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 "Following the line of least resistance makes both men and rivers crooked" This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: MVaughan4@ucok.edu [mailto:MVaughan4@ucok.edu] Sent: Monday, March 06, 2006 8:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Speakers of native English As opposed to those speakers of non-native English! :) Mel Melville B. Vaughan, Ph. D. Assistant Professor Department of Biology University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 Date: Mon, 6 Mar 2006 16:26:51 -0000 From: "Marshall Terry Dr, Consultant Histopathologist" "One can't press too hard or too soft." This is the sort of expression that only native English speakers understand. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo.rogerson <@t> waht.swest.nhs.uk Tue Mar 7 05:44:18 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Mar 7 05:44:33 2006 Subject: [Histonet] Speakers of native English Message-ID: Why Terry cat got yer tongue? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 "Following the line of least resistance makes both men and rivers crooked" This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: Tuesday, March 07, 2006 11:33 AM To: Kemlo Rogerson; MVaughan4@ucok.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Speakers of native English Um - well - err - yes (ish). Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:kemlo.rogerson@waht.swest.nhs.uk] Sent: 07 March 2006 08:17 To: 'MVaughan4@ucok.edu'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Speakers of native English If Terry had said a Native (noun) of England rather than native (adj) English it may have worked but that's semantics; we know what he meant. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 "Following the line of least resistance makes both men and rivers crooked" This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: MVaughan4@ucok.edu [mailto:MVaughan4@ucok.edu] Sent: Monday, March 06, 2006 8:29 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Speakers of native English As opposed to those speakers of non-native English! :) Mel Melville B. Vaughan, Ph. D. Assistant Professor Department of Biology University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 Date: Mon, 6 Mar 2006 16:26:51 -0000 From: "Marshall Terry Dr, Consultant Histopathologist" "One can't press too hard or too soft." This is the sort of expression that only native English speakers understand. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Tue Mar 7 05:53:24 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Tue Mar 7 05:53:27 2006 Subject: [Histonet] Speakers of native English Message-ID: Only a Native English speaker could understand that:-) Gotcha. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk Why Terry cat got yer tongue? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 From kemlo.rogerson <@t> waht.swest.nhs.uk Tue Mar 7 05:59:48 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Mar 7 05:59:58 2006 Subject: [Histonet] Speakers of native English Message-ID: Touch? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 "Following the line of least resistance makes both men and rivers crooked" This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: Tuesday, March 07, 2006 11:53 AM To: Kemlo Rogerson; MVaughan4@ucok.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Speakers of native English Only a Native English speaker could understand that:-) Gotcha. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk Why Terry cat got yer tongue? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 From petepath <@t> yahoo.com Tue Mar 7 06:26:05 2006 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Tue Mar 7 06:26:09 2006 Subject: [Histonet] Speakers of native English Message-ID: <20060307122605.45942.qmail@web30409.mail.mud.yahoo.com> It sounds like the English natives are restless. From mparker <@t> epl-inc.com Tue Mar 7 06:44:07 2006 From: mparker <@t> epl-inc.com (Mary Parker) Date: Tue Mar 7 06:44:06 2006 Subject: [Histonet] Linear Stainer Message-ID: If anyone has a Hacker linear stainer, I would very much like to hear about you staining protocol. Thanks From mparker <@t> epl-inc.com Tue Mar 7 08:25:15 2006 From: mparker <@t> epl-inc.com (Mary Parker) Date: Tue Mar 7 08:25:11 2006 Subject: [Histonet] Linear Stainer Message-ID: We are having uneven staining mostly in the brain, liver and heart. It is sporadic. We have made sure that the slides are properly de-waxed and that all solutions are to the proper level. We have stained some slides by hand, using the same tap water as we use on the stainer and the sections were not uneven. If you could share with me the timing schedule and type of Hematoxylin and Eosin you are using, I would appreciate it very much. Mary -----Original Message----- From: Jim Staruk [mailto:jstaruk@masshistology.com] Sent: Tuesday, March 07, 2006 9:14 AM To: Mary Parker Subject: RE: [Histonet] Linear Stainer We got one. What's your question? Jim ______________________ Jim Staruk Mass Histology Service www.masshistology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mary Parker Sent: Tuesday, March 07, 2006 7:44 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Linear Stainer If anyone has a Hacker linear stainer, I would very much like to hear about you staining protocol. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rcharles <@t> state.pa.us Tue Mar 7 08:49:21 2006 From: rcharles <@t> state.pa.us (Charles, Roger) Date: Tue Mar 7 08:49:29 2006 Subject: [Histonet] frozen sections Message-ID: <12E4E17FEF6EBE4BAE95BEB3CDCB5700025F13C1@enhbgpri04.backup> Try super frost plus slides. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Helen Beard Sent: Tuesday, March 07, 2006 5:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] frozen sections I have been given some stored mouse brains (-70C), that were fixed by paraformaldehyde perfusion, cryosectioned, wrapped in foil and stored for about a year. A couple of attempts at IHC on previously cut and stored frozen sections and freshly cut sections have resulted in the sections floating off at various stages. The blocks were difficult to cut (?tissue dried out) with the sections being picked up on silanised slides. I tried air drying for 1 hour & air dry/cold acetone/air drying prior to IHC. Should I air dry for an extended period, try super frost plus slides or is the fact that the tissue may have dried out causing most of my difficulties? Any suggestions as to how I can prevent the sections floating off during IHC would be greatly appreciated. Helen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Tue Mar 7 09:39:17 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Mar 7 09:39:28 2006 Subject: [Histonet] Hall or fouchet stain Message-ID: <001501c641fd$4e704630$eeeea8c0@SERVER01> Can somebody help me with the shelflive of this reagens'? 25% trichloracetic acid Fouchet reagens: 100ml 25% trichloracetic acid and 10ml 10%ferrichlorid Thank you Gudrun Lang Akh Linz Austria From john_ronan <@t> merck.com Tue Mar 7 09:41:00 2006 From: john_ronan <@t> merck.com (Ronan, John) Date: Tue Mar 7 09:41:36 2006 Subject: [Histonet] Bar Code System Message-ID: <9815A361655CFD429F892D0F08BA330402B969@usctmx1119.merck.com> Hi, Is there a reliable bar code system that any one is using to lable specimen containers, slides and labels in Histology especially on the pharmaceutical side? Linda Linda, Leica's cassette and slide printer will print barcodes. You can use a database program like ACCESS or Filemaker Pro to print labels for specimen containers. You will also need to barcode the shelves that the specimens will be on. You Can then use the database program to accession, track, and archive. John Ronan Research Biologist Merck & Co. ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------------ From kemlo.rogerson <@t> waht.swest.nhs.uk Tue Mar 7 09:27:03 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Mar 7 09:46:40 2006 Subject: [Histonet] entering multiple specimens Message-ID: The i's have it? Or not? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 "Following the line of least resistance makes both men and rivers crooked" This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Robyn Vazquez [mailto:vazquezr@ohsu.edu] Sent: Tuesday, March 07, 2006 3:16 PM To: TMcNemar@lmhealth.org; histonet@pathology.swmed.edu; Terry.Marshall@rothgen.nhs.uk; JWEEMS@sjha.org; kemlo.rogerson@waht.swest.nhs.uk Subject: RE: [Histonet] entering multiple specimens Hello, Yes I am female...yes I said it I am female...But you have got to ponder. Why is Bobby (male) spelt this way and female spelt Bobbie? Same thing with Tony/Toni, Andi/Andy and so on and so on...just a question... Robyn From kemlo.rogerson <@t> waht.swest.nhs.uk Tue Mar 7 09:15:38 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Mar 7 09:46:41 2006 Subject: [Histonet] entering multiple specimens Message-ID: I'm a man; you could be right about Terry, that's a girl's name isn't it? Psst Robyn hasn't fessed up to being a girl yet. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Robyn Vazquez [mailto:vazquezr@ohsu.edu] Sent: Tuesday, March 07, 2006 3:11 PM To: TMcNemar@lmhealth.org; histonet@pathology.swmed.edu; Terry.Marshall@rothgen.nhs.uk; JWEEMS@sjha.org; kemlo.rogerson@waht.swest.nhs.uk Subject: RE: [Histonet] entering multiple specimens Hello, Thank you Kemlo for coming to my rescue!!! :>) And Terry I apologize for hurting your ears with my English....:>) ;>) Have a great day gentleman (presuming your both men)... Robyn OHSU From histology <@t> gradymem.org Tue Mar 7 08:42:01 2006 From: histology <@t> gradymem.org (histology@gradymem.org) Date: Tue Mar 7 09:46:47 2006 Subject: [Histonet] CLIA REGS RE: Grossing: Chime In Message-ID:

I've been off a couple of days and just read these mes CLIA grossing requirements I spoke to a OKC.  I was told that the reason that specimen was considered high complexity testing was that the description becomes part of the pathology report.

Angie Barnett, HTL(ASCP)
Grady Memorial Hospital
Pathol
histology@gradymem.org <

----- Original Message -----

From: Roxanne Soto <GODSGIRLNOW@

Date: Saturday, March 4, 2006 8:36 [Histonet] CLIA REGS R

> So in reading the link that you sent me including have a degree including all of the
> ----- Original Mes From: Lance
> Erickson< mailto:Lance.Erickson@intermountainmail.org>
> Soto<mailto:godsgirlnow@msn.com> ;
> Histonet@pathology.swmed.edu<mailto:Hist onet@pathology.swmed.edu>
> Sent: Friday, RE: [Histonet
>
> www.cms.hhs.gov/clia<http://www.cms. hhs.gov/clia> is the link to
> the CLIA '88 inf intrepreti
> Ap
> www.cms.hhs.gov/CLIA/downloads/apcsubm.pdf<http: //www.cms.hhs.gov/CLIA/downloads/apcsubm.pdf> under qualif 493.1489 (b)(7) i whether it is color, measu considered high complexity testing an type of testing must qualify under this sec new CAP question ANP 11610 was instated and is ef 2005. CAP must abide by CLIA regulations and CLIA i Center for Medicare and Medicaid Services which is a sectio US government's department of Health and Human Services. So you would like to maintain your CLIA license and CAP certification and complexi gross examina Guidelines ?493.1489 the technical supervisor may delegate to individuals ?493.1489 the responsibility for the physical examina tion/description, including color, weight, measurement and other ch which a supervisor gross examina technical supervisor is supervision but is responsible for reported. All physical examinations/de including color, weight, measurement and other c of the tissue; or other mechanical procedures performed absence of the technical supervisor by individuals qualified under ?493.1489 should be reviewed within 24 hours by the technical superv by individu appropriate."
> Anatom Primary Children's Medical Cen City, UT
>
> Message-----
> From: histonet-bounces@l ists.utsouthwestern.edu<mailto:histonet-
> bo
Of Roxanne S 1:21 PM
LuckG@empirehealth.org<mailto:LuckG@empireh ealth.org>;
> Charles.Embrey@carle.com& lt;mailto:Charles.Embrey@carle.com>; Histonet@pathol ogy.swmed.edu<mailto:Histonet@pathology.swmed.edu=2 6gt RE: Gros
> Greg, for your comment-that is exa to ask,
> but yo did.
>
> This is what I need to know, as well, and I have had a very time trying to find the answer by looking at t CLIA website.
>
> Rox
>
> ____________ _______________________ 5F_ _______________________ 5F_
> < LuckG@empirehealth.org<mailto:LuckG@empirehealth.org 26g
<Charles.Embrey@carle.com<mailto:Charles. Embrey@carle.com>>,
> Histonet@patho logy.swmed.edu<mailto:Histonet@pathology.swmed.edu 26g Grossing: 10:47:45 -0800 <
> >In answer to th
> requirements for
> "non-pathologists
> >grossing" you cited the s states < patient samples" > how that
> >strictly translates to "gros
> t transfer of an entire sp
> cassettes with a < description and simple specimen prep and set in
> reducing the
> the sample to be processed; e.g.
> with a
> scalpel.
> >Wo uldn't simple (where the entire specimen is
> submitted >decision has
> specimen to

> >not submit for micro exam be
> analogous for
> ex
> >Micro lab aid who does this on tissue cultures.
> "Grossing" by
> your com between shave prostatectomy.
> >Where in CLIA does it's 'text' specifically state >"grossing" and who can or c
> CLIA's definition of
> g
> >For those of us less familiar with th federal register
> can you
> provide > measuring cores and > >cassette count as you have
> said yes it
& Thanks,
> >Greg
> & gt;Greg Luck, BS, HT(ASCP)
> >Anatomic Pathol Center
>Spokane, WA 509.473.7077
> &
> >luckg@empirehealth. >www.deaconessmedicalcenter.org
&
> >
> >
>
> >-----Original Message-----
> >From [mailto:Charles.Embrey@carle.com]
>Sent: Friday, March 03, 2006 7:10 AM
& gt; >To:
> Histonet@pathology.swmed.edu& lt;mailto:Histonet@pathology.swmed.edu>
>
> > >
>
> >From: Charl >Sent: Friday, March 03, 2006 9
> >To: 'Roxanne Soto'
> >Subject: RE: [Histonet] CLIA REGS
> > for non-pa
> &g it's a punch
& shave.
> > < '88 states "On or before April 24 1995 (I) high school
> graduate or
& >equivalent; and (b) have documentation of training
> appropriate for
> the test
> >performed before analyzing pat specimens"............. that date
> >it requires an associat in a biological or
> chemical science or
> >medical laboratory technology -or- qualify as a
>bachelor'
> earned a <
> >degree in a chemical, or clinical
> laboratory science. >
> >ref. CLIA '88 493.
> >
> >As to your questi on: "Does measuring cores and putting
> them in a > >dissection of an entire colon, the req same.
> >It falls under the CLI Complexity Testing
> Personnel Qualifications, < BR> > >Federal Register VOl. 60, No. 78, April 1995, > < instruction detailing w
> >grossed wi pathologists'
> observation. Dir
> means that
> >the pathologist literally watches over your shoulder
> while you
& Indirect means that consult. >
& concerned: Florida has one of <
> stringent
> > systems in the US. I fully expect,
> now tha
> Pathologists'
> >Assistants ar closely at and may limit g P.A.s, >Pa is
> just a g
> >be surprised to see it hap too distant future.
> >
& >Charles Embrey, PA(ASCP)
> >Carle C linic
> >Urbana, IL
> > < >
> & bounces@lists.utsouthwestern. edu<mailto:histonet-bounces@lists.utsouthwestern.edu& gt; >[mailto:histonet-bounces@lists.utsouthw estern.edu] On
> Behalf Of
> Roxanne So 6:39 AM HISTONET@PATHOLOGY.SW MED.EDU<mailto:HISTONET@PATHOLOGY.SWMED.EDU> > > Would someo
> the CLIA
&g
> > and out) please contact regard of
> tissue-----what is
> > grossing per se--does measuring cores and putting
> cassette
> > as grossing? What education level doesn one
> hav to
> have
> >in
> &
> aides, but our lab degrees and they
> cores.......
& advance
> > Roxanne Soto < >_________________ _______________________ 5F_
&
> & gt;http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >
> >___________________ _______________________ 5F_
>
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
& _______________________ _______________________ 5F
> Histonet@lists.utsouthwestern.edu<mailto:Histonet@lis ts. http://lists.utsouthwestern.edu/mailma n/listinfo/histonet<http://lists.utsouthwestern.edu/mailm an/ _____ _______________________ 5F_
> Hist Histonet@lists.utsouthwestern.ed http://lists.utsouthwestern.edu/mailman/listinfo
> From kemlo.rogerson <@t> waht.swest.nhs.uk Tue Mar 7 05:46:34 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Mar 7 09:46:55 2006 Subject: [Histonet] entering multiple specimens Message-ID: "post to which she (I take Robyn to be female)" Why? Be very, very careful before you answer that; remember when you've started to dig a hole it's best to stop before it's too deep. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 "Following the line of least resistance makes both men and rivers crooked" This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: Tuesday, March 07, 2006 11:42 AM To: Kemlo Rogerson; Robyn Vazquez; TMcNemar@lmhealth.org; histonet@pathology.swmed.edu; JWEEMS@sjha.org Subject: RE: [Histonet] entering multiple specimens It was not the use of English that was the problem, but the complete lack of reference to the post to which she (I take Robyn to be female) was replying. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:kemlo.rogerson@waht.swest.nhs.uk] Sent: 07 March 2006 08:24 To: Marshall Terry Dr, Consultant Histopathologist; Robyn Vazquez; TMcNemar@lmhealth.org; histonet@pathology.swmed.edu; JWEEMS@sjha.org Subject: RE: [Histonet] entering multiple specimens Makes complete sense to me. What he's saying is that you use an accession number to denote the Patient (ie. 123456) followed by a suffix to denote the sample (ie. 123456/7) the suffix being the number of the sample (ie in this case number 7); you can then use another suffix to denote the block and then even the slide (123456/7/1/2; Patient 123456 sample 7, block 1, slide 2). Hang on in Robyn I understand you perfectly; nouns, adjectives, what the hell it's only language. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 "Following the line of least resistance makes both men and rivers crooked" This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: Monday, March 06, 2006 4:12 PM To: Robyn Vazquez; TMcNemar@lmhealth.org; histonet@pathology.swmed.edu; JWEEMS@sjha.org Subject: RE: [Histonet] entering multiple specimens It would help if you just gave a teeny weenie hint as to what you are talking about. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Robyn Vazquez [mailto:vazquezr@ohsu.edu] Sent: 06 March 2006 15:20 To: TMcNemar@lmhealth.org; histonet@pathology.swmed.edu; JWEEMS@sjha.org Subject: RE: [Histonet] entering multiple specimens Hello, You don't do it that way, for the fact of keeping tract of the number of patients you process. You usually follow it with a 1-2-3 or a-b-c. Robyn OHSU _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo.rogerson <@t> waht.swest.nhs.uk Tue Mar 7 02:23:31 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Mar 7 09:46:59 2006 Subject: [Histonet] entering multiple specimens Message-ID: Makes complete sense to me. What he's saying is that you use an accession number to denote the Patient (ie. 123456) followed by a suffix to denote the sample (ie. 123456/7) the suffix being the number of the sample (ie in this case number 7); you can then use another suffix to denote the block and then even the slide (123456/7/1/2; Patient 123456 sample 7, block 1, slide 2). Hang on in Robyn I understand you perfectly; nouns, adjectives, what the hell it's only language. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 "Following the line of least resistance makes both men and rivers crooked" This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: Monday, March 06, 2006 4:12 PM To: Robyn Vazquez; TMcNemar@lmhealth.org; histonet@pathology.swmed.edu; JWEEMS@sjha.org Subject: RE: [Histonet] entering multiple specimens It would help if you just gave a teeny weenie hint as to what you are talking about. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Robyn Vazquez [mailto:vazquezr@ohsu.edu] Sent: 06 March 2006 15:20 To: TMcNemar@lmhealth.org; histonet@pathology.swmed.edu; JWEEMS@sjha.org Subject: RE: [Histonet] entering multiple specimens Hello, You don't do it that way, for the fact of keeping tract of the number of patients you process. You usually follow it with a 1-2-3 or a-b-c. Robyn OHSU _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From runyanc <@t> mail.nih.gov Tue Mar 7 09:58:26 2006 From: runyanc <@t> mail.nih.gov (Runyan, Caroline (NIH/NIMH) [F]) Date: Tue Mar 7 09:58:30 2006 Subject: [Histonet] c-fos immuno in nonhuman primate Message-ID: Hello, Has anyone stained primate tissue for c-fos? If so, what antibody was used and how was the signal:noise? I've been trying to pick one out to try but none look like they would be too great. Thanks, Caroline From Terry.Marshall <@t> rothgen.nhs.uk Tue Mar 7 09:50:36 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Tue Mar 7 09:58:36 2006 Subject: [Histonet] entering multiple specimens Message-ID: Hi Robyn, glad you *are* a little girl, I would so hate it if Kemlo ever had one over on me. But to answer your question, it's so Tony can dress in pink and not be confused with being a girl. Some other confusion may ensue of course, but this sketch is already too silly. Hello, Yes I am female...yes I said it I am female...But you have got to ponder. Why is Bobby (male) spelt this way and female spelt Bobbie? Same thing with Tony/Toni, Andi/Andy and so on and so on...just a question... Robyn Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk From Terry.Marshall <@t> rothgen.nhs.uk Tue Mar 7 09:45:18 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Tue Mar 7 09:58:37 2006 Subject: [Histonet] entering multiple specimens Message-ID: Teri is a little girl version - as in Terri Hatcher, but Terry is short for either Terrence or Teresa. In my case, it's my given name, and the only thing that's short is me. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:kemlo.rogerson@waht.swest.nhs.uk] Sent: 07 March 2006 15:16 To: 'Robyn Vazquez'; histonet@pathology.swmed.edu; Marshall Terry Dr, Consultant Histopathologist Subject: RE: [Histonet] entering multiple specimens I'm a man; you could be right about Terry, that's a girl's name isn't it? Psst Robyn hasn't fessed up to being a girl yet. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Robyn Vazquez [mailto:vazquezr@ohsu.edu] Sent: Tuesday, March 07, 2006 3:11 PM To: TMcNemar@lmhealth.org; histonet@pathology.swmed.edu; Terry.Marshall@rothgen.nhs.uk; JWEEMS@sjha.org; kemlo.rogerson@waht.swest.nhs.uk Subject: RE: [Histonet] entering multiple specimens Hello, Thank you Kemlo for coming to my rescue!!! :>) And Terry I apologize for hurting your ears with my English....:>) ;>) Have a great day gentleman (presuming your both men)... Robyn OHSU From JWEEMS <@t> sjha.org Tue Mar 7 09:19:45 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Mar 7 09:58:54 2006 Subject: [Histonet] entering multiple specimens Message-ID: <83AACDB0810528418AA106F9AE9B7F7E01305A77@sjhaexc02.sjha.org> Because Toni wears pink booties and Tony wears blue -- and so on and so on....:>) Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 404-851-7831 - fax -----Original Message----- From: Robyn Vazquez [mailto:vazquezr@ohsu.edu] Sent: Tuesday, March 07, 2006 10:16 AM To: TMcNemar@lmhealth.org; histonet@pathology.swmed.edu; Terry.Marshall@rothgen.nhs.uk; Weems, Joyce; kemlo.rogerson@waht.swest.nhs.uk Subject: RE: [Histonet] entering multiple specimens Hello, Yes I am female...yes I said it I am female...But you have got to ponder. Why is Bobby (male) spelt this way and female spelt Bobbie? Same thing with Tony/Toni, Andi/Andy and so on and so on...just a question... Robyn Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From vazquezr <@t> ohsu.edu Tue Mar 7 09:16:22 2006 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Tue Mar 7 09:58:56 2006 Subject: [Histonet] entering multiple specimens Message-ID: Hello, Yes I am female...yes I said it I am female...But you have got to ponder. Why is Bobby (male) spelt this way and female spelt Bobbie? Same thing with Tony/Toni, Andi/Andy and so on and so on...just a question... Robyn From vazquezr <@t> ohsu.edu Tue Mar 7 09:10:51 2006 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Tue Mar 7 09:58:58 2006 Subject: [Histonet] entering multiple specimens Message-ID: Hello, Thank you Kemlo for coming to my rescue!!! :>) And Terry I apologize for hurting your ears with my English....:>) ;>) Have a great day gentleman (presuming your both men)... Robyn OHSU From kemlo.rogerson <@t> waht.swest.nhs.uk Tue Mar 7 07:49:13 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Mar 7 09:59:02 2006 Subject: [Histonet] entering multiple specimens Message-ID: Um........ Eloquently put but I think you are struggling! Then if you knew Robyn was a female your remark could have been less patronising don't you think? After all we Brits are known for our chivalry. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 "Following the line of least resistance makes both men and rivers crooked" This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: Tuesday, March 07, 2006 12:03 PM To: Kemlo Rogerson; Robyn Vazquez; TMcNemar@lmhealth.org; histonet@pathology.swmed.edu; JWEEMS@sjha.org Subject: RE: [Histonet] entering multiple specimens >From a name site: "Robyn is an uncommon male first name as it was not ranked for males of all ages in the 1990 U.S. Census. Robyn is a somewhat common surname, ranking 68580 out of 88799 for people of all ages in the 1990 U.S. Census." >From my own experience, in Australia and NZ, Robyn was a common name - all female. Ergo, "(I take Robyn to be female)" Note, *not* Robyn is a female. Robyn - HELP. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:kemlo.rogerson@waht.swest.nhs.uk] Sent: 07 March 2006 11:47 To: Marshall Terry Dr, Consultant Histopathologist; Kemlo Rogerson; Robyn Vazquez; TMcNemar@lmhealth.org; histonet@pathology.swmed.edu; JWEEMS@sjha.org Subject: RE: [Histonet] entering multiple specimens "post to which she (I take Robyn to be female)" Why? Be very, very careful before you answer that; remember when you've started to dig a hole it's best to stop before it's too deep. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 "Following the line of least resistance makes both men and rivers crooked" This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: Tuesday, March 07, 2006 11:42 AM To: Kemlo Rogerson; Robyn Vazquez; TMcNemar@lmhealth.org; histonet@pathology.swmed.edu; JWEEMS@sjha.org Subject: RE: [Histonet] entering multiple specimens It was not the use of English that was the problem, but the complete lack of reference to the post to which she (I take Robyn to be female) was replying. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:kemlo.rogerson@waht.swest.nhs.uk] Sent: 07 March 2006 08:24 To: Marshall Terry Dr, Consultant Histopathologist; Robyn Vazquez; TMcNemar@lmhealth.org; histonet@pathology.swmed.edu; JWEEMS@sjha.org Subject: RE: [Histonet] entering multiple specimens Makes complete sense to me. What he's saying is that you use an accession number to denote the Patient (ie. 123456) followed by a suffix to denote the sample (ie. 123456/7) the suffix being the number of the sample (ie in this case number 7); you can then use another suffix to denote the block and then even the slide (123456/7/1/2; Patient 123456 sample 7, block 1, slide 2). Hang on in Robyn I understand you perfectly; nouns, adjectives, what the hell it's only language. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 "Following the line of least resistance makes both men and rivers crooked" This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: Monday, March 06, 2006 4:12 PM To: Robyn Vazquez; TMcNemar@lmhealth.org; histonet@pathology.swmed.edu; JWEEMS@sjha.org Subject: RE: [Histonet] entering multiple specimens It would help if you just gave a teeny weenie hint as to what you are talking about. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Robyn Vazquez [mailto:vazquezr@ohsu.edu] Sent: 06 March 2006 15:20 To: TMcNemar@lmhealth.org; histonet@pathology.swmed.edu; JWEEMS@sjha.org Subject: RE: [Histonet] entering multiple specimens Hello, You don't do it that way, for the fact of keeping tract of the number of patients you process. You usually follow it with a 1-2-3 or a-b-c. Robyn OHSU _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Tue Mar 7 06:02:50 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Tue Mar 7 09:59:10 2006 Subject: [Histonet] entering multiple specimens Message-ID: >From a name site: "Robyn is an uncommon male first name as it was not ranked for males of all ages in the 1990 U.S. Census. Robyn is a somewhat common surname, ranking 68580 out of 88799 for people of all ages in the 1990 U.S. Census." >From my own experience, in Australia and NZ, Robyn was a common name - all female. Ergo, "(I take Robyn to be female)" Note, *not* Robyn is a female. Robyn - HELP. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:kemlo.rogerson@waht.swest.nhs.uk] Sent: 07 March 2006 11:47 To: Marshall Terry Dr, Consultant Histopathologist; Kemlo Rogerson; Robyn Vazquez; TMcNemar@lmhealth.org; histonet@pathology.swmed.edu; JWEEMS@sjha.org Subject: RE: [Histonet] entering multiple specimens "post to which she (I take Robyn to be female)" Why? Be very, very careful before you answer that; remember when you've started to dig a hole it's best to stop before it's too deep. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 "Following the line of least resistance makes both men and rivers crooked" This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: Tuesday, March 07, 2006 11:42 AM To: Kemlo Rogerson; Robyn Vazquez; TMcNemar@lmhealth.org; histonet@pathology.swmed.edu; JWEEMS@sjha.org Subject: RE: [Histonet] entering multiple specimens It was not the use of English that was the problem, but the complete lack of reference to the post to which she (I take Robyn to be female) was replying. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:kemlo.rogerson@waht.swest.nhs.uk] Sent: 07 March 2006 08:24 To: Marshall Terry Dr, Consultant Histopathologist; Robyn Vazquez; TMcNemar@lmhealth.org; histonet@pathology.swmed.edu; JWEEMS@sjha.org Subject: RE: [Histonet] entering multiple specimens Makes complete sense to me. What he's saying is that you use an accession number to denote the Patient (ie. 123456) followed by a suffix to denote the sample (ie. 123456/7) the suffix being the number of the sample (ie in this case number 7); you can then use another suffix to denote the block and then even the slide (123456/7/1/2; Patient 123456 sample 7, block 1, slide 2). Hang on in Robyn I understand you perfectly; nouns, adjectives, what the hell it's only language. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 "Following the line of least resistance makes both men and rivers crooked" This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: Monday, March 06, 2006 4:12 PM To: Robyn Vazquez; TMcNemar@lmhealth.org; histonet@pathology.swmed.edu; JWEEMS@sjha.org Subject: RE: [Histonet] entering multiple specimens It would help if you just gave a teeny weenie hint as to what you are talking about. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Robyn Vazquez [mailto:vazquezr@ohsu.edu] Sent: 06 March 2006 15:20 To: TMcNemar@lmhealth.org; histonet@pathology.swmed.edu; JWEEMS@sjha.org Subject: RE: [Histonet] entering multiple specimens Hello, You don't do it that way, for the fact of keeping tract of the number of patients you process. You usually follow it with a 1-2-3 or a-b-c. Robyn OHSU _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Tue Mar 7 05:41:41 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Tue Mar 7 09:59:18 2006 Subject: [Histonet] entering multiple specimens Message-ID: It was not the use of English that was the problem, but the complete lack of reference to the post to which she (I take Robyn to be female) was replying. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:kemlo.rogerson@waht.swest.nhs.uk] Sent: 07 March 2006 08:24 To: Marshall Terry Dr, Consultant Histopathologist; Robyn Vazquez; TMcNemar@lmhealth.org; histonet@pathology.swmed.edu; JWEEMS@sjha.org Subject: RE: [Histonet] entering multiple specimens Makes complete sense to me. What he's saying is that you use an accession number to denote the Patient (ie. 123456) followed by a suffix to denote the sample (ie. 123456/7) the suffix being the number of the sample (ie in this case number 7); you can then use another suffix to denote the block and then even the slide (123456/7/1/2; Patient 123456 sample 7, block 1, slide 2). Hang on in Robyn I understand you perfectly; nouns, adjectives, what the hell it's only language. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 "Following the line of least resistance makes both men and rivers crooked" This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: Monday, March 06, 2006 4:12 PM To: Robyn Vazquez; TMcNemar@lmhealth.org; histonet@pathology.swmed.edu; JWEEMS@sjha.org Subject: RE: [Histonet] entering multiple specimens It would help if you just gave a teeny weenie hint as to what you are talking about. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Robyn Vazquez [mailto:vazquezr@ohsu.edu] Sent: 06 March 2006 15:20 To: TMcNemar@lmhealth.org; histonet@pathology.swmed.edu; JWEEMS@sjha.org Subject: RE: [Histonet] entering multiple specimens Hello, You don't do it that way, for the fact of keeping tract of the number of patients you process. You usually follow it with a 1-2-3 or a-b-c. Robyn OHSU _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Tue Mar 7 10:02:56 2006 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Tue Mar 7 10:03:42 2006 Subject: [Histonet] Ki67 on mouse kidney tissue Message-ID: <005f01c64200$9953d880$112b5c9f@Carlos> For me, Labvision's SP6 Ki67 worked very well on unfixed, snap-frozen sections of mouse skeletal muscle. Fixing sections in 10% formalin in PBS, for 10mins, prior to immunostaining gave good reproducible results; much better than methanol:acetone 1:1. It also works well on perfuse-fixed snap-frozen, cryostat sections. It works well on pwax for me, also. NB: The BD-Pharmingen mouse monoclonal Ki67 is equally good; also, I can dilute it by a factor of x1000, rather than x100 for the Labvision Ab. You can see pwax pics of the two Abs here http://www.immunoportal.com/ in the image gallery From PMonfils <@t> Lifespan.org Tue Mar 7 10:13:50 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Tue Mar 7 10:13:56 2006 Subject: [Histonet] entering multiple specimens Message-ID: <09C945920A6B654199F7A58A1D7D1FDE0171767F@lsexch.lsmaster.lifespan.org> ... and then, there was a boy named Sue. Thanks to the late Johnny Cash. From catherine.scott <@t> mpiresearch.com Tue Mar 7 10:42:24 2006 From: catherine.scott <@t> mpiresearch.com (Catherine Scott) Date: Tue Mar 7 10:41:28 2006 Subject: [Histonet] Open Position Message-ID: Good Afternoon All, We currently have a mangers position open. I have listed the job responsibilities and required experience below. Manager, Plastics Laboratory MPI Research, Mattawan, MI has an immediate opening for a Manager in our newly constructed Plastics Laboratory. This position is accountable for providing leadership and direction of the Plastic laboratory by making study assignments, scheduling staff, providing training, interfacing with sponsors and generally facilitating operations. The manager apprises senior management of issues requiring attention, and makes recommendations to benefit departmental operations. The manager may interact with inside and outside sources to facilitate and ensure effectiveness of the department's operations and may act as liaison between MPI staff, management, Pathologists and Study Directors. The manager may perform any of all the following administrative functions: interviewing employment candidates, preparing and conducting employee performance reviews and other administrative tasks as assigned. The candidate must have 5+ years of histology experience along with (ASCP) HT/ HTL certification. The candidate must have experience working with GMA, MMA, cardiac stents, undecalcified bone, histomorphometry and special stains. The successful candidate must be a self-starter, have leadership qualities and troubleshooting abilities. If you are interested please contact me directly or visit our web site at www.mpiresearch.com for more information. Thank you, Catherine Scott Catherine L. Scott B.A., HT (ASCP), ALAT Associate Director, Pathology Services 54943 North Main Street Mattawan, Michigan 49071-9399 USA Telephone: 269.668.3336 ext. 1218 Fax: 269.668.4151 email: catherine.scott@mpiresearch.com From vazquezr <@t> ohsu.edu Tue Mar 7 10:03:35 2006 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Tue Mar 7 10:59:53 2006 Subject: [Histonet] entering multiple specimens Message-ID: Bravo!!! :>) From gillian.2.brown <@t> gsk.com Tue Mar 7 10:08:38 2006 From: gillian.2.brown <@t> gsk.com (gillian.2.brown@gsk.com) Date: Tue Mar 7 10:59:57 2006 Subject: [Histonet] entering multiple specimens In-Reply-To: Message-ID: Sorry but if this line of chat is going to go on, and on and on........ would you mind changing the subject line so I can decide if I want to be amused or informed. Thanks Gill "Robyn Vazquez" Sent by: histonet-bounces@lists.utsouthwestern.edu 07-Mar-2006 15:16 To TMcNemar@lmhealth.org, histonet@pathology.swmed.edu, Terry.Marshall@rothgen.nhs.uk, JWEEMS@sjha.org, kemlo.rogerson@waht.swest.nhs.uk cc Subject RE: [Histonet] entering multiple specimens Hello, Yes I am female...yes I said it I am female...But you have got to ponder. Why is Bobby (male) spelt this way and female spelt Bobbie? Same thing with Tony/Toni, Andi/Andy and so on and so on...just a question... Robyn _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vazquezr <@t> ohsu.edu Tue Mar 7 10:06:27 2006 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Tue Mar 7 10:59:58 2006 Subject: [Histonet] entering multiple specimens Message-ID: Your right... From Terry.Marshall <@t> rothgen.nhs.uk Tue Mar 7 10:21:33 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Tue Mar 7 11:00:20 2006 Subject: [Histonet] entering multiple specimens Message-ID: Robyn, you are still doing it!!!! Bravo to what? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Robyn Vazquez [mailto:vazquezr@ohsu.edu] Sent: 07 March 2006 16:04 To: histonet@pathology.swmed.edu; Marshall Terry Dr, Consultant Histopathologist; kemlo.rogerson@waht.swest.nhs.uk Subject: RE: [Histonet] entering multiple specimens Bravo!!! :>) From Terry.Marshall <@t> rothgen.nhs.uk Tue Mar 7 10:21:55 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Tue Mar 7 11:00:22 2006 Subject: [Histonet] entering multiple specimens Message-ID: Who is right about what? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Robyn Vazquez [mailto:vazquezr@ohsu.edu] Sent: 07 March 2006 16:06 To: TMcNemar@lmhealth.org; histonet@pathology.swmed.edu; Marshall Terry Dr, Consultant Histopathologist; JWEEMS@sjha.org; kemlo.rogerson@waht.swest.nhs.uk Subject: RE: [Histonet] entering multiple specimens Your right... From ploykasek <@t> phenopath.com Tue Mar 7 10:30:26 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Tue Mar 7 11:00:26 2006 Subject: [Histonet] DCC antibody Message-ID: Anyone using BD Pharmigen DCC antibody clone clone G97-449 on FFPE human tissues? We have used this clone in the past & are having trouble with the last 2 lots we have received. Also, has anyone tried Novocastra's DCC clone DM51? Thank you for the help. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From mtitford <@t> aol.com Tue Mar 7 11:06:55 2006 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Tue Mar 7 11:07:13 2006 Subject: [Histonet] Histowrap & Toilet paper Message-ID: <8C81020ABF89D11-11B4-2B8DF@mblk-r17.sysops.aol.com> Donna Willis at Milestone Medical asked about a supplier of "Histowrap" It is available from: Obex Industries 178 Fairlawn Drive Buffalo NY 14226 Fax (413) 586 8803 Cat # is #225 I wrote to them once suggesting they sell it like toilet paper on rolls so pathologists could just tear of a square when they needed it instead of fumbling around with bloody, gloved hands, but I never heard back. I guess they thought "What does a redneck in Alabama know about "Histowrap"! Mike Titford USA PAthology Mobile AL USA From Terry.Marshall <@t> rothgen.nhs.uk Tue Mar 7 11:10:13 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Tue Mar 7 11:10:10 2006 Subject: [Histonet] Histowrap & Toilet paper Message-ID: A good idea. I tried to find a source of the paper they use for tea bags about 5 years ago, but with no success. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: mtitford@aol.com [mailto:mtitford@aol.com] Sent: 07 March 2006 17:07 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histowrap & Toilet paper Donna Willis at Milestone Medical asked about a supplier of "Histowrap" It is available from: Obex Industries 178 Fairlawn Drive Buffalo NY 14226 Fax (413) 586 8803 Cat # is #225 I wrote to them once suggesting they sell it like toilet paper on rolls so pathologists could just tear of a square when they needed it instead of fumbling around with bloody, gloved hands, but I never heard back. I guess they thought "What does a redneck in Alabama know about "Histowrap"! Mike Titford USA PAthology Mobile AL USA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tyler-wellington <@t> northwestern.edu Tue Mar 7 11:31:41 2006 From: tyler-wellington <@t> northwestern.edu (Tyler W.) Date: Tue Mar 7 11:31:47 2006 Subject: [Histonet] reply versus reply to all Message-ID: <20060307173141.9E6847E581@merle.it.northwestern.edu> sorry to sound rude as you all have helped me a lot, but i use this email account for important work emails and its a lot easier to miss an important one in between 15 bickering male-female jokes that i'm not participating in, nor are at all relavent to histology. this is an important job that we have and a clooged email inbox doesn't help us do it any better. private debates, of course are A-OK, but i just recieved 20 emails about some argument i have nothing to do with. i'm just confused. shouldn't this be a message board and not a list serve? why am i recieving all these messages? tyler w. From GDawson <@t> dynacaremilwaukee.com Tue Mar 7 11:32:25 2006 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Tue Mar 7 11:33:45 2006 Subject: [Histonet] entering multiple specimens Message-ID: <84A42E6F38BE8A45AAB03628C8C80D1204C121@dynams.dynacaremilwaukee.com> I'm sure I'll be called a jerk for putting in my 2 cents worth (as usual) but...time to exchange phone numbers or start instant messaging each other guys. These 2-6 word emails are filling up my inbox & the subject line is not even close anymore. Glen Dawson Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Robyn Vazquez Sent: Tuesday, March 07, 2006 10:06 AM To: TMcNemar@lmhealth.org; histonet@pathology.swmed.edu; Terry.Marshall@rothgen.nhs.uk; JWEEMS@sjha.org; kemlo.rogerson@waht.swest.nhs.uk Subject: RE: [Histonet] entering multiple specimens Your right... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> Stowers-Institute.org Tue Mar 7 11:33:37 2006 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Tue Mar 7 11:34:07 2006 Subject: [Histonet] Re: linear stainer issues Message-ID: Mary, Do you have the stainer set to do dipping up and down when it is put in solution? If I recall correctly with the Hacker stainer, there is a switch for agitation next to the power switch. I think using agitation gives a more even stain. We used 3 buckets of hematoxylin stain (hemalum) in order to have proper timing in our stain solution (~1.5 minutes/station). That also gave us more consistency than using only 1 bucket for a longer period of time. Make sure you have a bucket of DI water prior to the hematoxylin. That helps keep some of the tap water additives from messing up your hematoxylin stain solution. We successfully used the Richard Allen stains and the Shandon Instant Hematoxylin stain with our linear stainer. Which stain you use mostly depends on what color the pathologists are accustomed to seeing, and whether they are bothered by intestinal mucins tinting blue. Make sure you use an acetic acid differentiator rather than a hydrochloric acid differentiator on the unit. You can dip quickly enough when hand staining that hydrochloric acid alcohol decolorizes more evenly and is stopped more quickly in running water than what you can achieve on the linear stainer. Richard Allen (and perhaps others?) have commercial products that have acetic acid in alcohol and acetic acid in water that can help provide some measure of "differentiation"...it does not extract the stain like the hydrochloric acid alcohol solutions do. That's all I can think of at the moment. I hope this helps. Best wishes, Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64133 From TJJ <@t> Stowers-Institute.org Tue Mar 7 11:53:13 2006 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Tue Mar 7 11:53:29 2006 Subject: [Histonet] Re: Histowrap Message-ID: Cutting up lens paper to Histowrap size gives you the same product. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64133 From bill501 <@t> mindspring.com Tue Mar 7 12:02:47 2006 From: bill501 <@t> mindspring.com (Bill Blank) Date: Tue Mar 7 12:02:54 2006 Subject: [Histonet] Re: Histowrap In-Reply-To: References: Message-ID: We use end papers used for home permanents which are available at the local stores and are cheap. BB From jcline <@t> wchsys.org Tue Mar 7 12:24:15 2006 From: jcline <@t> wchsys.org (Joyce Cline) Date: Tue Mar 7 12:24:31 2006 Subject: [Histonet] FW: Needing information about recycling Message-ID: <003301c64214$58344ec0$1d2a14ac@wchsys.org> I use the CBG system. CBG should be able to answer your questions, they have been most helpful to me. I only recycle alcohol and xylene substitute. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brandi Farris Sent: Sunday, March 05, 2006 10:02 PM To: histonet@lists.utsouthwestern.edu Cc: Patricia Bates Subject: [Histonet] Needing information about recycling Our histology laboratory is looking at recycler from CBG that processes alcohol, xylene and formalin and we're interested in feedback and answers from users of the system. Are there any fumes with the recycler while it is processing formalin? Can we use the recycled formalin for patient specimens or is it for use only on the processor?Will you please tell us the cost and procedure for buffering? Can you estimate about how much tech time is spent with the system? Your response is appreciated. Thank you, Brandi Farris Boone Hospital Center Columbia, MO 65201 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From liz <@t> premierlab.com Tue Mar 7 12:27:09 2006 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Mar 7 12:27:19 2006 Subject: [Histonet] CD69, CD94 and Bax antibodies for mouse tissue Message-ID: <000001c64214$bf248aa0$c6d48a80@Chlipala> Hello everyone I was wondering what was a good source for the following antibodies for mouse tissue. Any suggestions would be helpful. thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 From gu.lang <@t> gmx.at Tue Mar 7 12:34:38 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Mar 7 12:34:48 2006 Subject: [Histonet] Hall or fouchet stain Message-ID: <002f01c64215$ccb52d90$eeeea8c0@SERVER01> Can somebody help me with the shelflive of this reagens'? 25% trichloracetic acid Fouchet reagens: 100ml 25% trichloracetic acid and 10ml 10%ferrichlorid Thank you Gudrun Lang Akh Linz Austria From detmar <@t> mshri.on.ca Tue Mar 7 12:44:57 2006 From: detmar <@t> mshri.on.ca (Jacqui Detmar) Date: Tue Mar 7 12:45:09 2006 Subject: [Histonet] CD69, CD94 and Bax antibodies for mouse tissue Message-ID: I have used the BaxNT antibody from Upstate (06-499)for both IHC and Western blotting with good results...wildtype tissues (placentae, mainly) are positive and the knockout tissues are negative. Epitomics has come out with a new rabbit monoclonal antibody for Bax which I am tempted to try, but have not yet done so. Jacqui -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Elizabeth Chlipala Sent: Tuesday, March 07, 2006 1:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD69, CD94 and Bax antibodies for mouse tissue Hello everyone I was wondering what was a good source for the following antibodies for mouse tissue. Any suggestions would be helpful. thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From unja <@t> cns.umass.edu Tue Mar 7 13:38:56 2006 From: unja <@t> cns.umass.edu (UnJa L. Hayes) Date: Tue Mar 7 13:39:01 2006 Subject: [Histonet] Vital nerve tissue stain (in vivo) Message-ID: <1141760336.440de150b876b@mail-www2.oit.umass.edu> Dear Histonet, Does anyone know of a stain I could use in vivo that would help visualize nerves so as to make them easier to locate? I need to cut specific nerves in the lower abdominal area of a small rodent. However, I'm having difficulty finding the nerves (or all of the branches of the nerves). I've searched the web, but couldn't find any simple stains that I could use in vivo . Any assistance would be greatly appreciated!!!! u -- UnJa L. Hayes, Ph.D. Center for Neuroendocrine Studies University of Massachusetts Department of Psychology Tobin Hall Amherst, MA 01003 Off: (413) 545-5955 Lab: (413) 545-0526 Fax: (413) 545-0996 From Rcartun <@t> harthosp.org Tue Mar 7 17:08:03 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Mar 7 17:08:36 2006 Subject: [Histonet] DAKO AEC+ Message-ID: Is anyone having problems with DAKO's AEC+ chromogen (tiny dots everywhere). Thanks! Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From ravishankar.nagarajan <@t> ranbaxy.com Tue Mar 7 21:30:09 2006 From: ravishankar.nagarajan <@t> ranbaxy.com (Ravishankar Nagarajan) Date: Tue Mar 7 21:33:07 2006 Subject: [Histonet] RE: Histonet Digest, Vol 28, Issue 11 Message-ID: <2533F91DF0FC3F48AE4919B3197765ED2E9619@GUR-PR-EXA1.Ranbaxy.com> Dear Group, Can anybody provide me the procedure for Wright Giemsa staining procedure for Bone marrow smears. We are performing the Wright's procedure here and we get inconsistent results here. Like the nuclei do not get stained. Could it be due to the fact that Wright's is only a cytoplasmic stain. Any suggestions would be welcome. I also include the procedure by which we do Wright's here. Wright's stain - 8 - 10 minutes Sorensen's buffer (pH - 7) - 8 -10 minutes Regards and Thanks, Dr.N>Ravishankar (i) The information contained in this e-mail message is intended only for the confidential use of the recipient(s) named above. This message is privileged and confidential. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. (ii) The sender confirms that Ranbaxy shall not be responsible if this email message is used for any indecent, unsolicited or illegal purposes, which are in violation of any existing laws and the same shall solely be the responsibility of the sender and that Ranbaxy shall at all times be indemnified of any civil and/ or criminal liabilities or consequences there. From jkiernan <@t> uwo.ca Wed Mar 8 00:53:35 2006 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Mar 8 00:53:39 2006 Subject: [Histonet] Vital nerve tissue stain (in vivo) References: <1141760336.440de150b876b@mail-www2.oit.umass.edu> Message-ID: <440E7F6F.B2E54CED@uwo.ca> Yes. Methylene blue. There is a large body of literature dating from Paul Ehrlich (1880s) to the 1990s and perhaps later. Vital staining with methylene blue should reveal the nerves in a surgical setting, but you will need to dig in the literature for practical details. This is a method that needs intelligence rather than a "protocol." For my humble contribution see Histochemistry 40: 51-57 (1974). John Kiernan London, Canada ----------------------------- "UnJa L. Hayes" wrote: > > Dear Histonet, > > Does anyone know of a stain I could use in vivo that would help visualize > nerves so as to make them easier to locate? > > I need to cut specific nerves in the lower abdominal area of a small > rodent. However, I'm having difficulty finding the nerves (or all of the > branches of the nerves). I've searched the web, but couldn't find any simple > stains that I could use in vivo . > > Any assistance would be greatly appreciated!!!! > > u > > -- > UnJa L. Hayes, Ph.D. > Center for Neuroendocrine Studies > University of Massachusetts > Department of Psychology > Tobin Hall > Amherst, MA 01003 > Off: (413) 545-5955 > Lab: (413) 545-0526 > Fax: (413) 545-0996 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Eric <@t> ategra.com Tue Mar 7 19:49:03 2006 From: Eric <@t> ategra.com (Eric Dye (ext 223)) Date: Wed Mar 8 04:54:30 2006 Subject: [Histonet] Histology Managers, Supervisors, and Bench Techs needed- Intervirewing and Hiring NOW Message-ID: Fellow-Histonetters I'm presently on a search for my best client companies seeking HistoTech Supervisors and Histo Bench Techs. These are mostly fulltime permanent positions. Most of my Histo Techs positions are clinical, although I do have a few Temporary and Research Histo Tech positions as well. Here are some of my Hottest jobs: 1. Ohio (Full-time, Perm, Bench, 3rd Shift) 2. Ohio (Full-time, Perm, MANAGER) 3. Ohio (full-time, Perm,Bench, 3rd Shift) 4. Colorado (Full-Time, Perm, Bench, 3rd Shift) 5. Rhode Island (Full-time, Perm, Bench, 2nd Shift) 6. New Jersey (Full-time, Perm, Bench) 7. Massachusetts (Boston) (Part-time, Bench) 8. New York (Long Island) (Full-time, Perm, SUPERVISOR) 9. Illinois (Full-time, Perm, SUPERVISOR) 10. Virginia(South) (Full-time, Perm, Bench) 11. Virginia (Full-time, Perm, LEAD TECH) 12. West Virginia (Full-time, Perm, Bench) The clients are currently interviewing candidates - so if you are interested, please call me today at 1-800-466-9919 ext 223 Thank you, Eric Dye-Sr Allied Healthcare Recruiter 1-800-466-9919 ext 223 --------------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing 800 466 9919 ext 223 - voice 407 671 6075 - fax eric@ategra.com To Learn More About Ategra: http://www.ategra.com ---------------------------------------------------------------- Note: this message is intended for: Fellow-Histonetters at histonet@lists.utsouthwestern.edu ---------------------------------------------------------------- ---------------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. ---------------------------------------------------------------- From arvind <@t> nbrc.res.in Wed Mar 8 05:58:19 2006 From: arvind <@t> nbrc.res.in (Arvind) Date: Wed Mar 8 05:53:15 2006 Subject: [Histonet] cryo soft tissue References: Message-ID: <000801c642a7$979bce60$aa00a8c0@nbrc.res.in> can any one provide detailed protocol for cryosectioning of human fetus brain ageing 3 months prenatal to 01 day postnatal using cryotome thanks in advance Arvind Singh Pundir National Brain Research Centre Manesar Gurgaon, HARYANA INDIA arvind@nbrc.res.in From naje1972 <@t> yahoo.com Wed Mar 8 06:49:34 2006 From: naje1972 <@t> yahoo.com (cynthia haynes) Date: Wed Mar 8 06:49:38 2006 Subject: [Histonet] negative immunohistochemistry control Message-ID: <20060308124934.59087.qmail@web33011.mail.mud.yahoo.com> Good Morning everyone, I have a question about immuno staining. I've been away from this type of staining for a while and now I am doing them again on a regular basis. Why do you run a negative control with each run? Are you only suppose to put the normal serum on negative control only? I've forgotten; would someone please answer these questions for me. Thanks in advance. Cynthia Haynes H.T. From Eric.C.Kellar <@t> questdiagnostics.com Wed Mar 8 07:23:21 2006 From: Eric.C.Kellar <@t> questdiagnostics.com (Kellar, Eric C) Date: Wed Mar 8 07:23:36 2006 Subject: [Histonet] RE: Negative Immunohistochemistry controls Message-ID: <6843061CE6B98E4B96590D4F299618F801583BAF@qdcws0117.us.qdx.com> Cynthia, Negative controls are used to check the presence of non-specific staining. An adequate (-) control consists of replacing the specific primary antibody by another antibody produced in the same species but with unrelated specificity. When using polyclonal antisera, a preimmune serum may be used. With monoclonal antibodies, normal mouse serum or non-immune mouse ascites is applied. They are used to evaluate the non-specific binding of that isotope to the cells. They are applied in the highest concentration that is used for the specific antibodies. In indirect or multi-step techniques the primary antibody may also be omitted to evaluate the non-specific binding of the other reagents. Eric C. Kellar Histology/Immunohistochemistry Quest Diagnostics, Inc Miami Good Morning everyone, I have a question about immuno staining. I've been away from this type of staining for a while and now I am doing them again on a regular basis. Why do you run a negative control with each run? Are you only suppose to put the normal serum on negative control only? I've forgotten; would someone please answer these questions for me. Thanks in advance. Cynthia Haynes H.T. ============================================================================== The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. ============================================================================== From cpomajzl <@t> cpllabs.com Wed Mar 8 07:35:54 2006 From: cpomajzl <@t> cpllabs.com (Chris Pomajzl) Date: Wed Mar 8 07:33:29 2006 Subject: [Histonet] negative immunohistochemistry control References: <20060308124934.59087.qmail@web33011.mail.mud.yahoo.com> Message-ID: <002101c642b5$3d31b760$26fca8c0@CSP> There are several alternatives for negative controls, but a common one is simply to use the buffer rinse in place of the primary antibody, thereby removing the first link in the chain. Another way is to keep everything the same and run the IHC stain on negative tissue (no antigen present in the tissue). ----- Original Message ----- From: "cynthia haynes" To: Sent: Wednesday, March 08, 2006 6:49 AM Subject: [Histonet] negative immunohistochemistry control > Good Morning everyone, I have a question about immuno > staining. I've been away from this type of staining > for a while and now I am doing them again on a regular > basis. Why do you run a negative control with each > run? Are you only suppose to put the normal serum on > negative control only? I've forgotten; would someone > please answer these questions for me. Thanks in > advance. > > Cynthia Haynes H.T. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Eric.C.Kellar <@t> questdiagnostics.com Wed Mar 8 07:48:58 2006 From: Eric.C.Kellar <@t> questdiagnostics.com (Kellar, Eric C) Date: Wed Mar 8 07:49:21 2006 Subject: [Histonet] Correction RE: Negative Immunohistochemistry controls Message-ID: <6843061CE6B98E4B96590D4F299618F801583BB0@qdcws0117.us.qdx.com> Cynthia, "They are used to evaluate the non-specific binding of that 'epitope' to the cells" I typed much too fast and neglected to proof read! Sorry about that! Eric C. Kellar Histology/Immunohistochemistry Quest Diagnostics, Inc. Miami ============================================================================== The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. ============================================================================== From rjbuesa <@t> yahoo.com Wed Mar 8 07:51:35 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 8 07:51:39 2006 Subject: [Histonet] negative immunohistochemistry control In-Reply-To: <20060308124934.59087.qmail@web33011.mail.mud.yahoo.com> Message-ID: <20060308135135.78870.qmail@web61222.mail.yahoo.com> Cynthia: The idea of the negative control is to try to determine if any reaction detected in the case is due to specific or unspecific binding. You don't need to run a negative slide for each antibody you are going to test on the case, you only need a negative slide per case, no matter how many antibodies you run. The only requirement is that the negative slide has to come from the same tissue tested. What I used to do was to add buffer instead of the primary Ab and all the other reagents the same as in the slides run for specific Abs. You could also add non-immune globuline instead of the antibody but in this case you would need 1 negative control for each type Ab (1 negative for monoclonal Abs and 1 for polyclonal Abs). I hope this will help you. Ren? J. cynthia haynes wrote: Good Morning everyone, I have a question about immuno staining. I've been away from this type of staining for a while and now I am doing them again on a regular basis. Why do you run a negative control with each run? Are you only suppose to put the normal serum on negative control only? I've forgotten; would someone please answer these questions for me. Thanks in advance. Cynthia Haynes H.T. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Mail Bring photos to life! New PhotoMail makes sharing a breeze. From ree3 <@t> leicester.ac.uk Wed Mar 8 08:08:29 2006 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Wed Mar 8 08:08:35 2006 Subject: [Histonet] negative immuno histochemistry control Message-ID: For polyclonal antibodies use species specific(rabbit,goatetcetc) normal serum, or pre-immune if available: for mouse monoclonals use the specific isotypic e.g. IgG2a; simply omitting the primary antibody or replacing it with diluent alone is no control. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of cynthia haynes Sent: 08 March 2006 12:50 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] negative immunohistochemistry control Good Morning everyone, I have a question about immuno staining. I've been away from this type of staining for a while and now I am doing them again on a regular basis. Why do you run a negative control with each run? Are you only suppose to put the normal serum on negative control only? I've forgotten; would someone please answer these questions for me. Thanks in advance. Cynthia Haynes H.T. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wfubmc.edu Wed Mar 8 08:12:47 2006 From: mward <@t> wfubmc.edu (Martha Ward) Date: Wed Mar 8 08:13:56 2006 Subject: [Histonet] Outsourcing transcription Message-ID: <61135F0455D33347B5AAE209B903A30412C89490@EXCHVS2.medctr.ad.wfubmc.edu> I am posting this for our manager. Our administration would like us to explore outsourcing our Pathology transcription and we would like to hear from anyone who has done this: vendor, cost, turnaround times. We currently use CoPath and the vendor would have to be compatible with this. Thanks in advance for your help. Martha Ward Wake Forest University Baptist Medical Center Dept. of Pathology Winston-Salem, NC 27157 From cpomajzl <@t> cpllabs.com Wed Mar 8 08:21:09 2006 From: cpomajzl <@t> cpllabs.com (Chris Pomajzl) Date: Wed Mar 8 08:18:36 2006 Subject: [Histonet] negative immunohistochemistry control References: <20060308135135.78870.qmail@web61222.mail.yahoo.com> Message-ID: <003b01c642bb$8c1baf60$26fca8c0@CSP> I don't understand the idea of only one negative control per case, as opposed to each antibody. How does one evaluate non-specific staining of antibodies that do not have a negative control? What if non-specific staining is inherent to a certain antibody? For example, the new CAP question regarding staining of endogenous biotin, particularly in kidney and liver - if you do not run a negative control for that specific antibody, how do you know if there is non-specific staining? ----- Original Message ----- From: "Rene J Buesa" To: "cynthia haynes" ; Sent: Wednesday, March 08, 2006 7:51 AM Subject: Re: [Histonet] negative immunohistochemistry control > Cynthia: > The idea of the negative control is to try to determine if any reaction detected in the case is due to specific or unspecific binding. > You don't need to run a negative slide for each antibody you are going to test on the case, you only need a negative slide per case, no matter how many antibodies you run. The only requirement is that the negative slide has to come from the same tissue tested. > > What I used to do was to add buffer instead of the primary Ab and all the other reagents the same as in the slides run for specific Abs. You could also add non-immune globuline instead of the antibody but in this case you would need 1 negative control for each type Ab (1 negative for monoclonal Abs and 1 for polyclonal Abs). > I hope this will help you. > Ren? J. > > cynthia haynes wrote: > Good Morning everyone, I have a question about immuno > staining. I've been away from this type of staining > for a while and now I am doing them again on a regular > basis. Why do you run a negative control with each > run? Are you only suppose to put the normal serum on > negative control only? I've forgotten; would someone > please answer these questions for me. Thanks in > advance. > > Cynthia Haynes H.T. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Yahoo! Mail > Bring photos to life! New PhotoMail makes sharing a breeze. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Wed Mar 8 08:24:30 2006 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Wed Mar 8 08:25:48 2006 Subject: [Histonet] negative immuno histochemistry control Message-ID: <84A42E6F38BE8A45AAB03628C8C80D1204C2C6@dynams.dynacaremilwaukee.com> Although this is a good point, I don't think this is optimal unless the normal serum or pre-immune that is used for the negative is from the exact same animal/supernatant that the antibody is drawn from with the antibody seperated out. Throwing in something "else" is bringing another factor into the equation. How is a person to know that the "exceptable" negative was collected correctly or that it is not contaminated. That being said, I myself do the above to remain CAP compliant. I used to do the antibody diluent for whichever antibody I was using for the negative control & I still think it's the most logical thing to do. By using diluent as the negative & keeping all other steps/reagents the same as the antibody you are running, you are ensuring that it is, indeed, something in the concentrated antibody that is causing staining not seen on the negative. Just My Opinion, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Edwards, R.E. Sent: Wednesday, March 08, 2006 8:08 AM To: cynthia haynes; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] negative immuno histochemistry control For polyclonal antibodies use species specific(rabbit,goatetcetc) normal serum, or pre-immune if available: for mouse monoclonals use the specific isotypic e.g. IgG2a; simply omitting the primary antibody or replacing it with diluent alone is no control. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of cynthia haynes Sent: 08 March 2006 12:50 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] negative immunohistochemistry control Good Morning everyone, I have a question about immuno staining. I've been away from this type of staining for a while and now I am doing them again on a regular basis. Why do you run a negative control with each run? Are you only suppose to put the normal serum on negative control only? I've forgotten; would someone please answer these questions for me. Thanks in advance. Cynthia Haynes H.T. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From la.sebree <@t> hosp.wisc.edu Wed Mar 8 08:30:45 2006 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Wed Mar 8 08:30:51 2006 Subject: [Histonet] negative immunohistochemistry control Message-ID: CAP states that for panels, running one negative control using the harshest protocol suffices. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chris Pomajzl Sent: Wednesday, March 08, 2006 8:21 AM To: HISTONET; Rene J Buesa Subject: Re: [Histonet] negative immunohistochemistry control I don't understand the idea of only one negative control per case, as opposed to each antibody. How does one evaluate non-specific staining of antibodies that do not have a negative control? What if non-specific staining is inherent to a certain antibody? For example, the new CAP question regarding staining of endogenous biotin, particularly in kidney and liver - if you do not run a negative control for that specific antibody, how do you know if there is non-specific staining? ----- Original Message ----- From: "Rene J Buesa" To: "cynthia haynes" ; Sent: Wednesday, March 08, 2006 7:51 AM Subject: Re: [Histonet] negative immunohistochemistry control > Cynthia: > The idea of the negative control is to try to determine if any reaction detected in the case is due to specific or unspecific binding. > You don't need to run a negative slide for each antibody you are going to test on the case, you only need a negative slide per case, no matter how many antibodies you run. The only requirement is that the negative slide has to come from the same tissue tested. > > What I used to do was to add buffer instead of the primary Ab and all the other reagents the same as in the slides run for specific Abs. You could also add non-immune globuline instead of the antibody but in this case you would need 1 negative control for each type Ab (1 negative for monoclonal Abs and 1 for polyclonal Abs). > I hope this will help you. > Ren? J. > > cynthia haynes wrote: > Good Morning everyone, I have a question about immuno > staining. I've been away from this type of staining > for a while and now I am doing them again on a regular > basis. Why do you run a negative control with each > run? Are you only suppose to put the normal serum on > negative control only? I've forgotten; would someone > please answer these questions for me. Thanks in > advance. > > Cynthia Haynes H.T. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Yahoo! Mail > Bring photos to life! New PhotoMail makes sharing a breeze. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Wed Mar 8 08:34:41 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Mar 8 08:34:33 2006 Subject: [Histonet] FW: STATLINE -- Special Report: CMS Announces MUE Proposal Will Be Withdrawn Message-ID: <83AACDB0810528418AA106F9AE9B7F7E01305A9B@sjhaexc02.sjha.org> Good news for now! STATLINE March 6, 2006 * ? 2006 College of American Pathologists Special Report CMS Announces MUE Proposal Will Be Withdrawn MUE Proposal no Longer Scheduled to be Implemented in July At a meeting today of the Physician Payment Advisory Commission (PPAC), CMS officials said that the current Medically Unbelievable Edits (MUE) proposal is no longer scheduled to be implemented in July as previously planned. The proposal will be revised and re-submitted for public review and comment. This announcement followed concerns raised by the Chairman of the Physician Payment Advisory Commission Dr. Ronald Castellanos who stated that the current edits could have significant negative impacts on patient care. Testifying before the Commission was Dr. William Rogers, who stated that he had learned about the problems with the current MUE proposal from the College of American Pathologists in a meeting last week. He had discussed these concerns with the MUE Project manager at CMS. The PPAC recommended "the CMS withdraw the proposal to create a list of MUEs and resubmit through the normal rulemaking process and to work closely with the medical community in this effort." The motion was unanimously accepted. The CMS decision to withdraw the MUE proposal comes in direct response to an intense lobbying campaign by a CAP-led coalition of national and state pathology societies. The AMA, numerous medical specialty societies, and the American Clinical Laboratory Association (ACLA) also strongly advocated for the withdrawal of the MUE proposal. The College and its coalition partners have pressed CMS to withdraw the MUE proposal and have argued that the agency should be required to utilize the formal rulemaking process to develop sweeping unit of service limits on all pathology and clinical laboratory services. The College has also asked the agency to provide the rationale and methodology utilized to develop the MUE proposal. We are awaiting a written notification from the contractor withdrawing the proposal. CAP will continue to keep members informed on further details of CMS's plans in this area. _____ Information About This Service The CAP member mailing list is closed and confidential. Its purpose is to distribute important news and information to CAP members. Individual members are not able to post messages to the list. Visit the CAP Web site for additional news and information. If you have comments or questions regarding this mailing list, contact John Carter at jcarter@cap.org. To remove yourself from this list, go to http://leda.cap.org/UM/U.asp?B1.98.9473.85954 and you will be removed immediately. Thank you. ? 2006, College of American Pathologists 325 Waukegan Road Northfield, IL 60093 Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From Jackie.O'Connor <@t> abbott.com Wed Mar 8 08:49:23 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed Mar 8 08:49:49 2006 Subject: [Histonet] negative immunohistochemistry control Message-ID: I'm not in a clinical setting - but I use peptide inhibition as a negative control for each antibody. JO'C "Chris Pomajzl" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/08/2006 08:21 AM To: "HISTONET" , "Rene J Buesa" cc: (bcc: Jackie M O'Connor/LAKE/GPRD/ABBOTT) Subject: Re: [Histonet] negative immunohistochemistry control I don't understand the idea of only one negative control per case, as opposed to each antibody. How does one evaluate non-specific staining of antibodies that do not have a negative control? What if non-specific staining is inherent to a certain antibody? For example, the new CAP question regarding staining of endogenous biotin, particularly in kidney and liver - if you do not run a negative control for that specific antibody, how do you know if there is non-specific staining? ----- Original Message ----- From: "Rene J Buesa" To: "cynthia haynes" ; Sent: Wednesday, March 08, 2006 7:51 AM Subject: Re: [Histonet] negative immunohistochemistry control > Cynthia: > The idea of the negative control is to try to determine if any reaction detected in the case is due to specific or unspecific binding. > You don't need to run a negative slide for each antibody you are going to test on the case, you only need a negative slide per case, no matter how many antibodies you run. The only requirement is that the negative slide has to come from the same tissue tested. > > What I used to do was to add buffer instead of the primary Ab and all the other reagents the same as in the slides run for specific Abs. You could also add non-immune globuline instead of the antibody but in this case you would need 1 negative control for each type Ab (1 negative for monoclonal Abs and 1 for polyclonal Abs). > I hope this will help you. > Ren? J. > > cynthia haynes wrote: > Good Morning everyone, I have a question about immuno > staining. I've been away from this type of staining > for a while and now I am doing them again on a regular > basis. Why do you run a negative control with each > run? Are you only suppose to put the normal serum on > negative control only? I've forgotten; would someone > please answer these questions for me. Thanks in > advance. > > Cynthia Haynes H.T. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Yahoo! Mail > Bring photos to life! New PhotoMail makes sharing a breeze. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mbmphoto <@t> gmail.com Wed Mar 8 09:19:00 2006 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Wed Mar 8 09:18:35 2006 Subject: [Histonet] myelin stain for 40um cryostat sections Message-ID: Could someone please recommend a good myelin stain protocol for 40um cryostat primate brain sections (both for fixed and fresh frozen sections? 10% NBF has been used on the fixed sections. I would greatly appreciate any information you could provide & thank you in advance. Yours Maria Bartola Mejia University California San Francisco (UCSF) Department of Neurosurgery San Francisco, CA 94103 Email: mbmphoto@gmail.com From froyer <@t> bitstream.net Wed Mar 8 09:27:37 2006 From: froyer <@t> bitstream.net (Ford Royer) Date: Wed Mar 8 09:27:51 2006 Subject: [Histonet] RE: Histonet Digest, Vol 28, Issue 11 In-Reply-To: <2533F91DF0FC3F48AE4919B3197765ED2E9619@GUR-PR-EXA1.Ranbaxy.com> Message-ID: <004201c642c4$d4da7e30$6f01a80a@fords> Are you hand staining or using an automated stainer? Back when I did BM smears, we found that they had to be run through the stainer 2 or 3 times due to the thickness of the smear. This did work well however; and we didn't have any problems with nuclear staining or "over staining". ~ Ford Ford M. Royer, MT(ASCP) Minnesota Medical Specialists Minneapolis, MN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ravishankar Nagarajan Sent: Tuesday, March 07, 2006 9:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 28, Issue 11 Dear Group, Can anybody provide me the procedure for Wright Giemsa staining procedure for Bone marrow smears. We are performing the Wright's procedure here and we get inconsistent results here. Like the nuclei do not get stained. Could it be due to the fact that Wright's is only a cytoplasmic stain. Any suggestions would be welcome. I also include the procedure by which we do Wright's here. Wright's stain - 8 - 10 minutes Sorensen's buffer (pH - 7) - 8 -10 minutes Regards and Thanks, Dr.N>Ravishankar (i) The information contained in this e-mail message is intended only for the confidential use of the recipient(s) named above. This message is privileged and confidential. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. (ii) The sender confirms that Ranbaxy shall not be responsible if this email message is used for any indecent, unsolicited or illegal purposes, which are in violation of any existing laws and the same shall solely be the responsibility of the sender and that Ranbaxy shall at all times be indemnified of any civil and/ or criminal liabilities or consequences there. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo.rogerson <@t> waht.swest.nhs.uk Wed Mar 8 09:35:53 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Mar 8 09:36:09 2006 Subject: [Histonet] myelin stain for 40um cryostat sections Message-ID: Myelin can be stained with Sudan 3 and iron haematoxylin. Cut frozen (10 U) sections and chromate in 2.5% potassium dichromate for 2 to 4 days, then wash. 5ml fresh 1% aqueous haematoxylin and 5 ml 4% iron alum fresh in a covered dish. Put your frozen sections in for 45 min at 60 degrees C. Agitate gently. Wash in water. Decolorise in 0.5% iron alum for 1 hr with agitation. Wash in water Treat with 1% borax/ 2.5% potassium ferricyanide solution for 10 min with agitation. Wash in water 6mls stock Sudan 2 (CI 12140) saturated in 99% isopropanol and dilute with 4 ml water, let stand 7 to 8 min and filter. Stain sections 10 min. wash in water Float onto slides, drain and mount in aqueous mountant. This is from Lillie and I used it decades ago with success; but the old ways are usually the best. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Maria Mejia [mailto:mbmphoto@gmail.com] Sent: Wednesday, March 08, 2006 3:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] myelin stain for 40um cryostat sections Could someone please recommend a good myelin stain protocol for 40um cryostat primate brain sections (both for fixed and fresh frozen sections? 10% NBF has been used on the fixed sections. I would greatly appreciate any information you could provide & thank you in advance. Yours Maria Bartola Mejia University California San Francisco (UCSF) Department of Neurosurgery San Francisco, CA 94103 Email: mbmphoto@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From john.r.spaull <@t> gsk.com Wed Mar 8 10:50:11 2006 From: john.r.spaull <@t> gsk.com (john.r.spaull@gsk.com) Date: Wed Mar 8 10:50:42 2006 Subject: [Histonet] Re negative immunohistochemistry control In-Reply-To: <200603081526.k28FQ15M003009@rtpsfimr002.glaxo.com> Message-ID: See Richard W. Burry Specificity Controls for Immunocytochemical Methods J. Histochem. Cytochem., Feb 2000; 48: 163 - 166. for a good discussion of controls in IHC. (And why peptide blocks aren't always what we think they are !) Regards, John John Spaull Target Discovery GlaxoSmithKline R&D Stevenage 01438 763296 ( fax 01438 764799 ) john.r.spaull@gsk.com From mweirauch <@t> crittenton.com Wed Mar 8 11:03:08 2006 From: mweirauch <@t> crittenton.com (Maray Weirauch) Date: Wed Mar 8 11:03:22 2006 Subject: [Histonet] Grossing questions Message-ID: I know that CLIA considers grossing to be high complexity testing and does not distinguish between types of specimens. How do you regard the requirements for documenting hardware (grossing hardware) that is sent to pathology or for techs involved in Cytology prepping that includes recording the specimen description? Both of these items do end up in a final report. From pedro.louro <@t> spcorp.com Wed Mar 8 10:47:59 2006 From: pedro.louro <@t> spcorp.com (Louro, Pedro) Date: Wed Mar 8 11:16:44 2006 Subject: [Histonet] looking for paraffin pitchers Message-ID: Hi everyone, I'm looking to purchase some new Electrical Paraffin Pitchers to dispense hot paraffin form the dispenser to the embedding centers. Any suggestions on what companies sell this product. Thanks for your suggestions in advance. Pedro ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From Charles.Embrey <@t> carle.com Wed Mar 8 11:51:39 2006 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Wed Mar 8 11:51:42 2006 Subject: [Histonet] Grossing questions Message-ID: This is something that has been hotly debated on the PA webserver lately. By law and Medicare billing requirements, a diagnosis, even a gross-only diagnosis must be rendered by a Pathologist. In many practices, the PA will do the gross exam and then the Pathologist will also physically examine the specimen prior to signing it out. In Medicare's eyes, charging for a gross examination (88400) that the pathologist doesn't actually do, is fraud. Cytotechs are qualified for high complexity testing and render a screening diagnosis. The prep work itself is something a really don't get involved with and maybe someone else out in histoland has more information on the subject. Charles Embrey, PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maray Weirauch Sent: Wednesday, March 08, 2006 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Grossing questions I know that CLIA considers grossing to be high complexity testing and does not distinguish between types of specimens. How do you regard the requirements for documenting hardware (grossing hardware) that is sent to pathology or for techs involved in Cytology prepping that includes recording the specimen description? Both of these items do end up in a final report. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NMargaryan <@t> childrensmemorial.org Wed Mar 8 12:07:43 2006 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Wed Mar 8 12:07:21 2006 Subject: [Histonet] negative immunohistochemistry control Message-ID: <63B8B599DE283148B92E83C78B32C15D01C76554@cmhexbe2.childrensmemorial.org> I really do not understand all these discussion, sorry. May in clinic different than in research? I always run negative control using a IgG isotype from the sheet in same concentration as a positive. But before to concentrate the antibody it is necessary to bring the protein concentration of negative control antibody to same protein concentration of your positive antibody. Run both, positive and negative side by side in same conditions. Only in this way you will be able to compare your staining. This is a way how I work; proof my staining and learn, Sincerely, Naira Naira V. Margaryan, D.V.M., Ph.D. Research Associate III Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3394 Tel: 773-755-6570/ext-8 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chris Pomajzl Sent: Wednesday, March 08, 2006 8:21 AM To: HISTONET; Rene J Buesa Subject: Re: [Histonet] negative immunohistochemistry control I don't understand the idea of only one negative control per case, as opposed to each antibody. How does one evaluate non-specific staining of antibodies that do not have a negative control? What if non-specific staining is inherent to a certain antibody? For example, the new CAP question regarding staining of endogenous biotin, particularly in kidney and liver - if you do not run a negative control for that specific antibody, how do you know if there is non-specific staining? ----- Original Message ----- From: "Rene J Buesa" To: "cynthia haynes" ; Sent: Wednesday, March 08, 2006 7:51 AM Subject: Re: [Histonet] negative immunohistochemistry control > Cynthia: > The idea of the negative control is to try to determine if any reaction detected in the case is due to specific or unspecific binding. > You don't need to run a negative slide for each antibody you are going to test on the case, you only need a negative slide per case, no matter how many antibodies you run. The only requirement is that the negative slide has to come from the same tissue tested. > > What I used to do was to add buffer instead of the primary Ab and all the other reagents the same as in the slides run for specific Abs. You could also add non-immune globuline instead of the antibody but in this case you would need 1 negative control for each type Ab (1 negative for monoclonal Abs and 1 for polyclonal Abs). > I hope this will help you. > Ren? J. > > cynthia haynes wrote: > Good Morning everyone, I have a question about immuno > staining. I've been away from this type of staining > for a while and now I am doing them again on a regular > basis. Why do you run a negative control with each > run? Are you only suppose to put the normal serum on > negative control only? I've forgotten; would someone > please answer these questions for me. Thanks in > advance. > > Cynthia Haynes H.T. > From anh2006 <@t> med.cornell.edu Wed Mar 8 12:12:06 2006 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Wed Mar 8 12:11:57 2006 Subject: [Histonet] negative immunohistochemistry control In-Reply-To: References: Message-ID: Peptide inhibibtion is a good control to ensure your antibody can be adsorbed by your antigen but it does not tell you whether or not your antibody will non-specifically bind to your tissue. It is a good but not a sufficient control. The best control is to stain tissue which DOES NOT express your antigen, it should be negative. >I'm not in a clinical setting - but I use peptide inhibition as a negative >control for each antibody. >JO'C > > > > >"Chris Pomajzl" >Sent by: histonet-bounces@lists.utsouthwestern.edu >03/08/2006 08:21 AM > > > To: "HISTONET" , "Rene J Buesa" > > cc: (bcc: Jackie M O'Connor/LAKE/GPRD/ABBOTT) > Subject: Re: [Histonet] negative immunohistochemistry control > > >I don't understand the idea of only one negative control per case, as >opposed to each antibody. How does one evaluate non-specific staining of >antibodies that do not have a negative control? What if non-specific >staining is inherent to a certain antibody? For example, the new CAP >question regarding staining of endogenous biotin, particularly in kidney >and >liver - if you do not run a negative control for that specific antibody, >how >do you know if there is non-specific staining? > >----- Original Message ----- >From: "Rene J Buesa" >To: "cynthia haynes" ; > >Sent: Wednesday, March 08, 2006 7:51 AM >Subject: Re: [Histonet] negative immunohistochemistry control > > >> Cynthia: >> The idea of the negative control is to try to determine if any >reaction >detected in the case is due to specific or unspecific binding. >> You don't need to run a negative slide for each antibody you are going >to test on the case, you only need a negative slide per case, no matter >how >many antibodies you run. The only requirement is that the negative slide >has >to come from the same tissue tested. >> >> What I used to do was to add buffer instead of the primary Ab and all >the other reagents the same as in the slides run for specific Abs. You >could >also add non-immune globuline instead of the antibody but in this case you >would need 1 negative control for each type Ab (1 negative for monoclonal >Abs and 1 for polyclonal Abs). >> I hope this will help you. >> Ren? J. >> >> cynthia haynes wrote: >> Good Morning everyone, I have a question about immuno >> staining. I've been away from this type of staining >> for a while and now I am doing them again on a regular >> basis. Why do you run a negative control with each >> run? Are you only suppose to put the normal serum on >> negative control only? I've forgotten; would someone >> please answer these questions for me. Thanks in >> advance. >> > > Cynthia Haynes H.T. >> > > -- From tmmrosla <@t> healtheast.org Wed Mar 8 12:11:39 2006 From: tmmrosla <@t> healtheast.org (Mrosla, Tina M) Date: Wed Mar 8 12:13:10 2006 Subject: [Histonet] microwave processor Message-ID: <22E6C4C32AFB3E44B7056C7EC3E1F20405312779@HECLUSTER.healtheast.loc> Our lab is looking to purchase our first microwave processor. We just had a demo from Milestone, and are interested in their Pathos model. We are wondering what others' experience has been with microwave processors and are open to demos from other companies. Thank you, Tina Mrosla (HTL) St. Joseph's Hospital St. Paul MN 55102 The information included in this e-mail message, including any attachments, is intended only for the person or organization to which it is addressed. This e-mail message may contain information that is privileged or confidential. If you receive this e-mail message and are not the intended recipient or responsible for delivering the message to the intended recipient, you may not use, disseminate, distribute or copy the information included in this e-mail and any attachments. If you received this e-mail message by mistake, please reply by e-mail and destroy all copies of this message and any attachments. Thank you. From histology <@t> gradymem.org Wed Mar 8 12:15:40 2006 From: histology <@t> gradymem.org (histology@gradymem.org) Date: Wed Mar 8 12:15:47 2006 Subject: [Histonet] Shandon AutoSharp V Message-ID: <10c533110c44dd.10c44dd10c5331@onenet.net> We have a Shandon Auto Sharp V Knife Sharpener in good working condition that we would like to get rid of. I will send it to the highest bidder plus shipping (or you can pick it up-40 mi. south of OKC) -- no minimum bid. Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org From anh2006 <@t> med.cornell.edu Wed Mar 8 12:21:50 2006 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Wed Mar 8 12:21:42 2006 Subject: [Histonet] negative immuno histochemistry control In-Reply-To: <84A42E6F38BE8A45AAB03628C8C80D1204C2C6@dynams.dynacaremilwaukee.com> References: <84A42E6F38BE8A45AAB03628C8C80D1204C2C6@dynams.dynacaremilwaukee.com> Message-ID: I respectfully disagree in most cases. But then again I am a stickler for negative controls and precise interpretation of results. Most of the background one would see with antibodies on tissues (assuming the concentrations are reasonable from 0.1-10 ug/ml and without ridiculous amplification and one is not using unpurified serum but instead somehow purified antibodies or tissue culture supernatant) is from Fc binding to endogenous Fc receptors in the tissue. Fc is much conserved from animal to animal within the same species, so using IgG from another animal is just fine and is in fact necessary. Antibody diluent is NOT ENOUGH to be robust negative control in your interpretation. All that tells you is what background your secondary antibody or detection system is contributing. It tells you NOTHING about the behaviour of your primary immunoglobulin species whatsoever. That being said, I am not in a clinical lab I don't know what the CAP regulations are specifically so my opinion is merely just that :) >Although this is a good point, I don't think this is optimal unless the >normal serum or pre-immune that is used for the negative is from the exact >same animal/supernatant that the antibody is drawn from with the antibody >seperated out. Throwing in something "else" is bringing another factor into >the equation. How is a person to know that the "exceptable" negative was >collected correctly or that it is not contaminated. > >That being said, I myself do the above to remain CAP compliant. I used to >do the antibody diluent for whichever antibody I was using for the negative >control & I still think it's the most logical thing to do. By using diluent >as the negative & keeping all other steps/reagents the same as the antibody >you are running, you are ensuring that it is, indeed, something in the >concentrated antibody that is causing staining not seen on the negative. > >Just My Opinion, > >Glen Dawson BS, HT & QIHC (ASCP) >IHC Manager >Milwaukee, WI -- From jfish <@t> gladstone.ucsf.edu Wed Mar 8 12:36:06 2006 From: jfish <@t> gladstone.ucsf.edu (Jo Dee Fish) Date: Wed Mar 8 12:36:12 2006 Subject: [Histonet] Neuronal cell culture blebbing Message-ID: <000001c642df$2996d990$8903010a@JFISH> Hello everyone, I have a question about neuronal cell cultures, and although I know we are a group who work primarily with tissues, sometimes these types of questions come to my lab and I am intrigued enough to try to find an answer. And I though I would consult with my fellow histonetters to see if any of you have any suggestions. An investigator who works with neuronal cell cultures has fixed them with 3.5% paraformaldehyde in 1xPBS solution with success, twice. Then the third time he saw blebbing on the cell membranes, and after consulting with our confocal microscopy expert, he changed to 4% paraformaldehyde (in, I believe, the same buffer) and had the same results. It turns out our expert has had the same problem with seemingly healthy cells that develop the blebbing upon fixation. My first instinct was that he needs to adjust the osmolarity of the solutions. Then I fell back to my electron microscopy experience and thought he may need to use a different fixative/buffering system, such as glutaraldehyde in cacodylate buffer. Does anyone here have a good protocol for fixing neurons in culture so that we can look at them as close to their natural state as possible. Seems like a simple question... Thanks! Jo Dee Jo Dee Fish Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 734-2567 Fax: (415) 355-0230 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 From jqb7 <@t> cdc.gov Wed Mar 8 12:32:40 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Wed Mar 8 12:37:41 2006 Subject: [Histonet] microwave processor Message-ID: We have the Sakura Xpress and are very pleased with it. In fact, it has exceeded my expectations regarding quality of processing and ease of use. Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mrosla, Tina M Sent: Wednesday, March 08, 2006 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] microwave processor Our lab is looking to purchase our first microwave processor. We just had a demo from Milestone, and are interested in their Pathos model. We are wondering what others' experience has been with microwave processors and are open to demos from other companies. Thank you, Tina Mrosla (HTL) St. Joseph's Hospital St. Paul MN 55102 The information included in this e-mail message, including any attachments, is intended only for the person or organization to which it is addressed. This e-mail message may contain information that is privileged or confidential. If you receive this e-mail message and are not the intended recipient or responsible for delivering the message to the intended recipient, you may not use, disseminate, distribute or copy the information included in this e-mail and any attachments. If you received this e-mail message by mistake, please reply by e-mail and destroy all copies of this message and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Wed Mar 8 12:51:59 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Wed Mar 8 12:58:58 2006 Subject: [Histonet] microwave processor Message-ID: I do want to say, however, that before we purchased the Xpress we used the TT/Mega by Milestone, also with great success. Of course, it is not an automated model so it is much more cumbersome to use (and not as user friendly) but the results were excellent. Jeanine Bartlett, BS, HT(ASCP) (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartlett, Jeanine Sent: Wednesday, March 08, 2006 1:33 PM To: Mrosla, Tina M; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] microwave processor We have the Sakura Xpress and are very pleased with it. In fact, it has exceeded my expectations regarding quality of processing and ease of use. Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mrosla, Tina M Sent: Wednesday, March 08, 2006 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] microwave processor Our lab is looking to purchase our first microwave processor. We just had a demo from Milestone, and are interested in their Pathos model. We are wondering what others' experience has been with microwave processors and are open to demos from other companies. Thank you, Tina Mrosla (HTL) St. Joseph's Hospital St. Paul MN 55102 The information included in this e-mail message, including any attachments, is intended only for the person or organization to which it is addressed. This e-mail message may contain information that is privileged or confidential. If you receive this e-mail message and are not the intended recipient or responsible for delivering the message to the intended recipient, you may not use, disseminate, distribute or copy the information included in this e-mail and any attachments. If you received this e-mail message by mistake, please reply by e-mail and destroy all copies of this message and any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lvalenta <@t> firstsourcelab.com Wed Mar 8 13:01:01 2006 From: lvalenta <@t> firstsourcelab.com (Valenta, Laura) Date: Wed Mar 8 12:59:26 2006 Subject: [Histonet] Remove from list Message-ID: <365FCDF2F3FC4745916F6034F4B4CC0A0E846A@fsfiles.FirstSource.local> Please take me off the email distribution list. I no longer wish to receive these messages. Laura Valenta From MDiCarlo <@t> KaleidaHealth.Org Wed Mar 8 14:14:39 2006 From: MDiCarlo <@t> KaleidaHealth.Org (DiCarlo, Margaret) Date: Wed Mar 8 14:14:48 2006 Subject: [Histonet] drawer for filing 5x7' slides? Message-ID: <9B4A77DF11463E4FB723D484214AE9BC286C00@KALEXMB02.KaleidaHealth.org> Histonetters, What kind of drawer or filing system do you use to file 5 x 7 inch slides? I currently stand them up and place cut sheets of material used for mailing packages in between each slide but the drawer isn't half filled and getting quite heavy for opening and closing. Any ideas would be greatly appreciated. Peggy DiCarlo HT (ASCP) Orthopedics Bone Lab Buffalo General Hospital 100 High St. Buffalo, NY 14203 716-859-1293 _____ Upgrade Your Email - Click here! CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From lmusunuri <@t> yahoo.com Wed Mar 8 14:45:41 2006 From: lmusunuri <@t> yahoo.com (Lakshmi musunuri) Date: Wed Mar 8 14:45:46 2006 Subject: [Histonet] Remove from list In-Reply-To: <365FCDF2F3FC4745916F6034F4B4CC0A0E846A@fsfiles.FirstSource.local> Message-ID: <20060308204541.32547.qmail@web54315.mail.yahoo.com> Please remove me from the list I do not want to be included in the list Thanks " __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From Don.Birgerson <@t> leica-microsystems.com Wed Mar 8 14:49:37 2006 From: Don.Birgerson <@t> leica-microsystems.com (Don.Birgerson@leica-microsystems.com) Date: Wed Mar 8 14:49:48 2006 Subject: [Histonet] drawer for filing 5x7' slides? In-Reply-To: <9B4A77DF11463E4FB723D484214AE9BC286C00@KALEXMB02.KaleidaHealth.org> Message-ID: Hi Peggy, There is a company called "Brain Research Laboratories". They carry odd size slides etc.. Their website www.brainresearchlab.com or phone is 617-9655544 in Newton MA. Good luck. Don Birgerson Leica Microsystems Technical Assistance Center Don.Birgerson@Leica-Microsystems.Com 1-800-248-0123 ext 5918 "DiCarlo, Margaret" Sent by: cc histonet-bounces@ lists.utsouthwest Subject ern.edu [Histonet] drawer for filing 5x7' slides? 03/08/2006 02:14 PM Histonetters, What kind of drawer or filing system do you use to file 5 x 7 inch slides? I currently stand them up and place cut sheets of material used for mailing packages in between each slide but the drawer isn't half filled and getting quite heavy for opening and closing. Any ideas would be greatly appreciated. Peggy DiCarlo HT (ASCP) Orthopedics Bone Lab Buffalo General Hospital 100 High St. Buffalo, NY 14203 716-859-1293 _____ < http://promos.hotbar.com/promos/promodll.dll?RunPromo&El=&SG=&RAND=34947&partner=spamblockerutility > Upgrade Your Email - Click here! CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From Extolseventeen <@t> aol.com Wed Mar 8 15:49:17 2006 From: Extolseventeen <@t> aol.com (Extolseventeen@aol.com) Date: Wed Mar 8 15:49:27 2006 Subject: [Histonet] New Certification Procedure for HT in 2007? Message-ID: <253.7aa9ab0.3140ab5d@aol.com> Hello Everyone: I have just received word that a passing score on the Written/computer portion of the HT certification exam will become invalid at the end of this year; with a discontinuation of the practical portion of the exam in 2007. Are there new requirements for 2007? If the practical was to be discontinued, I thought that would be big news, but I cannot find it anywhere on the ASCP/BOR web site(s). For a little clarification, I picked this up from a co-worker: at the time he passed his exam, he was under the "five year" rule to qualify with the appropriate practical. Now, it is my understanding, that should he fail his practical this year, he will have to take a completely new written exam in 2007 and NO PRACTICAL. This hardly seems fair. I cannot get any information on this development; but I can imagine that he's not the only one getting squeezed on this. Can anyone offer any info or advice? Should one just wait until 2007 to get this streamlined HT CERT? Thanks! Please advise, Barb From tkngflght <@t> yahoo.com Wed Mar 8 16:10:31 2006 From: tkngflght <@t> yahoo.com (Cheryl) Date: Wed Mar 8 16:10:37 2006 Subject: [Histonet] New Certification Procedure for HT in 2007? In-Reply-To: <253.7aa9ab0.3140ab5d@aol.com> Message-ID: <20060308221031.58131.qmail@web50906.mail.yahoo.com> Hey folks- I have heard of this change--they are including more chromes with poorly executed stains and tissue processing and asking troubleshooting questions to supplant the actual cutting and staining practical exam. It was explained to me by one of the heads of an accredited HT/HTL school while we were talking about other things. She didn't have the implimentation dates but the way it was explained-- it makes the HT/HTL exam more like the MLT/MT and CT exams (they don't have practical portions, either). She didn't mention the effects on those already in process for their exams. I'll try to find out where she got her information from--the schools are 'front line' as far as changes go. I have a healthy skepticism regarding the changes--is it a boon or a bust? I suspect it will take several years to know for sure. Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one tech at a time. 281.852.9457 Extolseventeen@aol.com wrote: Hello Everyone: I have just received word that a passing score on the Written/computer portion of the HT certification exam will become invalid at the end of this year; with a discontinuation of the practical portion of the exam in 2007. Are there new requirements for 2007? If the practical was to be discontinued, I thought that would be big news, but I cannot find it anywhere on the ASCP/BOR web site(s). For a little clarification, I picked this up from a co-worker: at the time he passed his exam, he was under the "five year" rule to qualify with the appropriate practical. Now, it is my understanding, that should he fail his practical this year, he will have to take a completely new written exam in 2007 and NO PRACTICAL. This hardly seems fair. I cannot get any information on this development; but I can imagine that he's not the only one getting squeezed on this. Can anyone offer any info or advice? Should one just wait until 2007 to get this streamlined HT CERT? Thanks! Please advise, Barb _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asmith <@t> mail.barry.edu Wed Mar 8 16:27:39 2006 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Wed Mar 8 16:27:45 2006 Subject: [Histonet] Reproductive Toxins Message-ID: <5D2189E74151CC42BEC02906BA8996322B919B@exchsrv01.barrynet.barry.edu> It is true that few plant or animal toxins are uses in histology labs. Cottonmouth mocassin venom is used to prepare the substrate for demonstrating phospholipase B. Fluoroescent-labelled banded krait (the "dust snake" in RIKKI-TIKKI-TAVI) is used to demonstrate nicotinic acetylcholine receptors. Ouabain (from an African tree) is used as a control in demonstrating sodium/potassium ATPase. Nevertheless, many things in a histology labe are toxic. Since MSDS's tell you that everything (including table sugar) is toxic, it is hard find the real toxicity data in them. (Hint: it is usually buried 2/3 of the way down the second page as LD50 (how much has a 50% chance of killing you) or TWA (the maximum concentration you can safely have in the air around you). Often the MSDS wont't give you either piece of information. I look up the toxicity of dyes in Dapson's HAZARDOUS MATERIALS IN THE HISTOPATHOLOGY LABORATORY. I look up the toxicity of everything else in Lewis's HAZARDOUS CHEMICALS DESK REFERENCE. The former is an indispensible reference; the latter is very nice to have. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Monday, March 06, 2006 4:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Reproductive Toxins Does anyone know how to tell if something in the Histo lab is a reproductive toxin or acute toxin? Some of the MSDS sheets are no help at all. Anyone else having this problem? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From pmerrick <@t> fhcrc.org Wed Mar 8 16:38:28 2006 From: pmerrick <@t> fhcrc.org (Merrick, Pixie E) Date: Wed Mar 8 16:38:32 2006 Subject: [Histonet] Storage of unstained slides/mold contamination Message-ID: <5597E353734D6F40BB80524918D08867020106DA@harpo.fhcrc.org> Hi, I work for a study that archieves unstained tumor slides. Currently the means of storage is in a nitrogen desiccator cabinet inside a coldroom. The problem is that we have had two mold contaminations and I believe there is a better way of storing these slides so that they do not get contaminated. I have a meeting scheduled soon where I will argue the on the topic of variability of immunohistochemical reactivity on stored paraffin slides and I would also like to provide solid evidence of room temperature storage with/without desiccator cabinet vs. cold room storage when it comes to mold. I have searched PubMed and various other journals with minimal results. Any suggestions?? Thank you, Pixie Merrick Pathology Data Coordinator Cancer Prevention Program 1100 Fairview Ave. N., M4-B402 Seattle, WA 98109-1024 Tel: 206.667.2832 Fax: 206.667.7850 www.fhcrc.org From ahaines <@t> vims.edu Wed Mar 8 16:43:11 2006 From: ahaines <@t> vims.edu (Ashley Haines) Date: Wed Mar 8 16:43:21 2006 Subject: [Histonet] Combining Hema 3 and immuno detection Message-ID: <001e01c64301$adf85840$be07468b@campus.vims.edu> Hi All, Can anyone recommend the best way to combine immunodetection (either via fluorescence or enzyme) and a differential stain such as Hema 3 (or another Wright Giemsa like stain)? I'm trying this with cytospins and have had poor luck. So far either the cell morphology is bad and the signal is good or the signal is nonexistent but the cell morphology is good! Thanks in advance! Ashley N. Haines, PhD. Postdoctoral Research Associate Virginia Institute of Marine Science Department of Environmental & Aquatic Animal Health PO Box 1346 Gloucester Point, VA 23062 804-684-7243 From LuckG <@t> empirehealth.org Wed Mar 8 16:48:48 2006 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Wed Mar 8 16:48:56 2006 Subject: [Histonet] Grossing questions Message-ID: Hello All, All gross only specimens (e.g. hardware, other prostheses or calculi) are not done by our PA's. These are done only by the pathologists (for the reasons stated by Charles below. Greg Luck, BS, HT(ASCP) Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 Phone 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmedicalcenter.org -----Original Message----- From: Charles.Embrey [mailto:Charles.Embrey@carle.com] Sent: Wednesday, March 08, 2006 9:52 AM To: Maray Weirauch Cc: Histonet@pathology.swmed.edu Subject: RE: [Histonet] Grossing questions This is something that has been hotly debated on the PA webserver lately. By law and Medicare billing requirements, a diagnosis, even a gross-only diagnosis must be rendered by a Pathologist. In many practices, the PA will do the gross exam and then the Pathologist will also physically examine the specimen prior to signing it out. In Medicare's eyes, charging for a gross examination (88400) that the pathologist doesn't actually do, is fraud. Cytotechs are qualified for high complexity testing and render a screening diagnosis. The prep work itself is something a really don't get involved with and maybe someone else out in histoland has more information on the subject. Charles Embrey, PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maray Weirauch Sent: Wednesday, March 08, 2006 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Grossing questions I know that CLIA considers grossing to be high complexity testing and does not distinguish between types of specimens. How do you regard the requirements for documenting hardware (grossing hardware) that is sent to pathology or for techs involved in Cytology prepping that includes recording the specimen description? Both of these items do end up in a final report. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bwhitaker <@t> brownpathology.com Wed Mar 8 17:13:48 2006 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Wed Mar 8 17:13:11 2006 Subject: [Histonet] Grossing questions In-Reply-To: Message-ID: <000e01c64305$f4e98540$3601a8c0@brownpathology.net> Ditto!! Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Luck, Greg D. Sent: Wednesday, March 08, 2006 4:49 PM To: 'Charles.Embrey'; Maray Weirauch Cc: Histonet@pathology.swmed.edu Subject: RE: [Histonet] Grossing questions Hello All, All gross only specimens (e.g. hardware, other prostheses or calculi) are not done by our PA's. These are done only by the pathologists (for the reasons stated by Charles below. Greg Luck, BS, HT(ASCP) Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 Phone 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmedicalcenter.org -----Original Message----- From: Charles.Embrey [mailto:Charles.Embrey@carle.com] Sent: Wednesday, March 08, 2006 9:52 AM To: Maray Weirauch Cc: Histonet@pathology.swmed.edu Subject: RE: [Histonet] Grossing questions This is something that has been hotly debated on the PA webserver lately. By law and Medicare billing requirements, a diagnosis, even a gross-only diagnosis must be rendered by a Pathologist. In many practices, the PA will do the gross exam and then the Pathologist will also physically examine the specimen prior to signing it out. In Medicare's eyes, charging for a gross examination (88400) that the pathologist doesn't actually do, is fraud. Cytotechs are qualified for high complexity testing and render a screening diagnosis. The prep work itself is something a really don't get involved with and maybe someone else out in histoland has more information on the subject. Charles Embrey, PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Maray Weirauch Sent: Wednesday, March 08, 2006 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Grossing questions I know that CLIA considers grossing to be high complexity testing and does not distinguish between types of specimens. How do you regard the requirements for documenting hardware (grossing hardware) that is sent to pathology or for techs involved in Cytology prepping that includes recording the specimen description? Both of these items do end up in a final report. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LuckG <@t> empirehealth.org Wed Mar 8 17:19:47 2006 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Wed Mar 8 17:19:56 2006 Subject: [Histonet] negative immuno histochemistry control Message-ID: Hello all, My approach is the essence of the negative control is to verify that serum (and its components without the addition of an antibody) from the same host species is not "on it's own" producing any non-specific background staining. This being said would suggest to me that applying of any other reagent on the negative slide except serum from the host species would not accomplish this. Thanks, Greg -----Original Message----- From: Dawson, Glen [mailto:GDawson@dynacaremilwaukee.com] Sent: Wednesday, March 08, 2006 6:25 AM To: Edwards, R.E.; cynthia haynes; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] negative immuno histochemistry control Although this is a good point, I don't think this is optimal unless the normal serum or pre-immune that is used for the negative is from the exact same animal/supernatant that the antibody is drawn from with the antibody seperated out. Throwing in something "else" is bringing another factor into the equation. How is a person to know that the "exceptable" negative was collected correctly or that it is not contaminated. That being said, I myself do the above to remain CAP compliant. I used to do the antibody diluent for whichever antibody I was using for the negative control & I still think it's the most logical thing to do. By using diluent as the negative & keeping all other steps/reagents the same as the antibody you are running, you are ensuring that it is, indeed, something in the concentrated antibody that is causing staining not seen on the negative. Just My Opinion, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Edwards, R.E. Sent: Wednesday, March 08, 2006 8:08 AM To: cynthia haynes; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] negative immuno histochemistry control For polyclonal antibodies use species specific(rabbit,goatetcetc) normal serum, or pre-immune if available: for mouse monoclonals use the specific isotypic e.g. IgG2a; simply omitting the primary antibody or replacing it with diluent alone is no control. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of cynthia haynes Sent: 08 March 2006 12:50 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] negative immunohistochemistry control Good Morning everyone, I have a question about immuno staining. I've been away from this type of staining for a while and now I am doing them again on a regular basis. Why do you run a negative control with each run? Are you only suppose to put the normal serum on negative control only? I've forgotten; would someone please answer these questions for me. Thanks in advance. Cynthia Haynes H.T. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Wed Mar 8 17:26:57 2006 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Mar 8 17:27:07 2006 Subject: [Histonet] Combining Hema 3 and immuno detection In-Reply-To: <001e01c64301$adf85840$be07468b@campus.vims.edu> Message-ID: <000001c64307$cb001490$c6d48a80@Chlipala> Ashley We have had good success in combining IHC with the chromagen DAB and a wide variety of special stains. There is a nice article in Histologic, I'm not sure what issue that covers IHC and PAS staining. There are some pictures on our web page that shows DAB IHC in combination with Acid Fast. We run the immuno first and then use whatever special stain we need as the counterstain. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ashley Haines Sent: Wednesday, March 08, 2006 3:43 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Combining Hema 3 and immuno detection Hi All, Can anyone recommend the best way to combine immunodetection (either via fluorescence or enzyme) and a differential stain such as Hema 3 (or another Wright Giemsa like stain)? I'm trying this with cytospins and have had poor luck. So far either the cell morphology is bad and the signal is good or the signal is nonexistent but the cell morphology is good! Thanks in advance! Ashley N. Haines, PhD. Postdoctoral Research Associate Virginia Institute of Marine Science Department of Environmental & Aquatic Animal Health PO Box 1346 Gloucester Point, VA 23062 804-684-7243 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1.1435 (20060308) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From LuckG <@t> empirehealth.org Wed Mar 8 17:30:41 2006 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Wed Mar 8 17:30:56 2006 Subject: [Histonet] negative immunohistochemistry control Message-ID: Chris, The CAP question is really designed to address the integrity of your detection system not the antibodies in general. If your negative control is truly negative (as it should be) any staing on the positive patient slide should be attributable to the antibody reagent that was applied as opposed to something unique to the tissue (excluding some tissue artifacts that seem to stain with many antibodies, like necrotic tissue components for example). Greg Luck, BS, HT(ASCP) Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 Phone 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmedicalcenter.org -----Original Message----- From: Chris Pomajzl [mailto:cpomajzl@cpllabs.com] Sent: Wednesday, March 08, 2006 6:21 AM To: HISTONET; Rene J Buesa Subject: Re: [Histonet] negative immunohistochemistry control I don't understand the idea of only one negative control per case, as opposed to each antibody. How does one evaluate non-specific staining of antibodies that do not have a negative control? What if non-specific staining is inherent to a certain antibody? For example, the new CAP question regarding staining of endogenous biotin, particularly in kidney and liver - if you do not run a negative control for that specific antibody, how do you know if there is non-specific staining? ----- Original Message ----- From: "Rene J Buesa" To: "cynthia haynes" ; Sent: Wednesday, March 08, 2006 7:51 AM Subject: Re: [Histonet] negative immunohistochemistry control > Cynthia: > The idea of the negative control is to try to determine if any reaction detected in the case is due to specific or unspecific binding. > You don't need to run a negative slide for each antibody you are going to test on the case, you only need a negative slide per case, no matter how many antibodies you run. The only requirement is that the negative slide has to come from the same tissue tested. > > What I used to do was to add buffer instead of the primary Ab and all the other reagents the same as in the slides run for specific Abs. You could also add non-immune globuline instead of the antibody but in this case you would need 1 negative control for each type Ab (1 negative for monoclonal Abs and 1 for polyclonal Abs). > I hope this will help you. > Ren? J. > > cynthia haynes wrote: > Good Morning everyone, I have a question about immuno > staining. I've been away from this type of staining > for a while and now I am doing them again on a regular > basis. Why do you run a negative control with each > run? Are you only suppose to put the normal serum on > negative control only? I've forgotten; would someone > please answer these questions for me. Thanks in > advance. > > Cynthia Haynes H.T. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Yahoo! Mail > Bring photos to life! New PhotoMail makes sharing a breeze. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From katri <@t> cogeco.ca Wed Mar 8 19:17:57 2006 From: katri <@t> cogeco.ca (Katri Tuomala) Date: Wed Mar 8 19:18:11 2006 Subject: [Histonet] negative immunohistochemistry control References: Message-ID: <006201c64317$4d10c010$6a9a9618@Katri> Almost in any given tissue (i.e. your positive control) has tissue components that do not and should not express the antigen you are staining for, so why run an extra slide for that? Doing the test slide by omitting the primary with the harshest retrieval, will give you all the information you need to show, that your procedure is working and if any non specific staining appears in your negative control slide, you would have to investigate further. Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "Andrea T. Hooper" To: "Jackie M O'Connor" ; "Histonet" Sent: Wednesday, March 08, 2006 1:12 PM Subject: Re: [Histonet] negative immunohistochemistry control Peptide inhibibtion is a good control to ensure your antibody can be adsorbed by your antigen but it does not tell you whether or not your antibody will non-specifically bind to your tissue. It is a good but not a sufficient control. The best control is to stain tissue which DOES NOT express your antigen, it should be negative. >I'm not in a clinical setting - but I use peptide inhibition as a negative >control for each antibody. >JO'C > > > > >"Chris Pomajzl" >Sent by: histonet-bounces@lists.utsouthwestern.edu >03/08/2006 08:21 AM > > > To: "HISTONET" , "Rene J > Buesa" > > cc: (bcc: Jackie M O'Connor/LAKE/GPRD/ABBOTT) > Subject: Re: [Histonet] negative immunohistochemistry > control > > >I don't understand the idea of only one negative control per case, as >opposed to each antibody. How does one evaluate non-specific staining of >antibodies that do not have a negative control? What if non-specific >staining is inherent to a certain antibody? For example, the new CAP >question regarding staining of endogenous biotin, particularly in kidney >and >liver - if you do not run a negative control for that specific antibody, >how >do you know if there is non-specific staining? > >----- Original Message ----- >From: "Rene J Buesa" >To: "cynthia haynes" ; > >Sent: Wednesday, March 08, 2006 7:51 AM >Subject: Re: [Histonet] negative immunohistochemistry control > > >> Cynthia: >> The idea of the negative control is to try to determine if any >reaction >detected in the case is due to specific or unspecific binding. >> You don't need to run a negative slide for each antibody you are going >to test on the case, you only need a negative slide per case, no matter >how >many antibodies you run. The only requirement is that the negative slide >has >to come from the same tissue tested. >> >> What I used to do was to add buffer instead of the primary Ab and all >the other reagents the same as in the slides run for specific Abs. You >could >also add non-immune globuline instead of the antibody but in this case you >would need 1 negative control for each type Ab (1 negative for monoclonal >Abs and 1 for polyclonal Abs). >> I hope this will help you. >> Ren? J. >> >> cynthia haynes wrote: >> Good Morning everyone, I have a question about immuno >> staining. I've been away from this type of staining >> for a while and now I am doing them again on a regular >> basis. Why do you run a negative control with each >> run? Are you only suppose to put the normal serum on >> negative control only? I've forgotten; would someone >> please answer these questions for me. Thanks in >> advance. >> > > Cynthia Haynes H.T. >> > > -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gmondragon <@t> gsopath.com Thu Mar 9 01:34:40 2006 From: gmondragon <@t> gsopath.com (Gus Mondragon) Date: Thu Mar 9 01:34:54 2006 Subject: [Histonet] CBG recycler Message-ID: <03B63DE44B5C014D84F9A9D8A968B5174C4D54@lithium.corp.gsopath.com> Hi Brandi; The CBG recycler is an excellent unit but you must understand a few things: It is not very fast and if your solvents are oversaturated it will take much longer. I run my basically 24 hrs to get about 12-15 gals of alcohol or xylene, that's about 3 runs It is very self contained, easy to operate with very little maintanance. Your lab staff must be very careful segregating the alcohols and xylenes in order to get good results. If your xylene has any traces of water from alcohol carry over the machine will not eliminate all of this water and your recyle product will turn milky when used. Always use a hydrometer to check your 100% which will be about 98%. We use some of this 98% to make 95% we just add water until the hydrometer reads 95% Use the mathematical formula in the CBG directions to check the Xylene purity, important. Once again, segregation, segregation of solvents it the only way the recovery is going to work x you. Gustave Mondragon Greensboro NC -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, March 08, 2006 10:41 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 28, Issue 12 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Histowrap (Bill Blank) 2. FW: Needing information about recycling (Joyce Cline) 3. CD69, CD94 and Bax antibodies for mouse tissue (Elizabeth Chlipala) 4. Hall or fouchet stain (Gudrun Lang) 5. RE: CD69, CD94 and Bax antibodies for mouse tissue (Jacqui Detmar) 6. Vital nerve tissue stain (in vivo) (UnJa L. Hayes) 7. DAKO AEC+ (Richard Cartun) 8. RE: Histonet Digest, Vol 28, Issue 11 (Ravishankar Nagarajan) 9. Re: Vital nerve tissue stain (in vivo) (John Kiernan) 10. Histology Managers, Supervisors, and Bench Techs needed- Intervirewing and Hiring NOW (Eric Dye (ext 223)) 11. cryo soft tissue (Arvind) 12. negative immunohistochemistry control (cynthia haynes) 13. RE: Negative Immunohistochemistry controls (Kellar, Eric C) 14. Re: negative immunohistochemistry control (Chris Pomajzl) 15. Correction RE: Negative Immunohistochemistry controls (Kellar, Eric C) 16. Re: negative immunohistochemistry control (Rene J Buesa) 17. RE: negative immuno histochemistry control (Edwards, R.E.) 18. Outsourcing transcription (Martha Ward) 19. Re: negative immunohistochemistry control (Chris Pomajzl) 20. RE: negative immuno histochemistry control (Dawson, Glen) 21. RE: negative immunohistochemistry control (Sebree Linda A.) 22. FW: STATLINE -- Special Report: CMS Announces MUE Proposal Will Be Withdrawn (Weems, Joyce) 23. Re: negative immunohistochemistry control (Jackie M O'Connor) 24. myelin stain for 40um cryostat sections (Maria Mejia) 25. RE: RE: Histonet Digest, Vol 28, Issue 11 (Ford Royer) 26. RE: myelin stain for 40um cryostat sections (Kemlo Rogerson) ---------------------------------------------------------------------- Message: 1 Date: Tue, 7 Mar 2006 12:02:47 -0600 From: Bill Blank Subject: [Histonet] Re: Histowrap To: Message-ID: Content-Type: text/plain; charset="us-ascii" ; format="flowed" We use end papers used for home permanents which are available at the local stores and are cheap. BB ------------------------------ Message: 2 Date: Tue, 7 Mar 2006 13:24:15 -0500 From: "Joyce Cline" Subject: [Histonet] FW: Needing information about recycling To: Message-ID: <003301c64214$58344ec0$1d2a14ac@wchsys.org> Content-Type: text/plain;charset="us-ascii" I use the CBG system. CBG should be able to answer your questions, they have been most helpful to me. I only recycle alcohol and xylene substitute. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brandi Farris Sent: Sunday, March 05, 2006 10:02 PM To: histonet@lists.utsouthwestern.edu Cc: Patricia Bates Subject: [Histonet] Needing information about recycling Our histology laboratory is looking at recycler from CBG that processes alcohol, xylene and formalin and we're interested in feedback and answers from users of the system. Are there any fumes with the recycler while it is processing formalin? Can we use the recycled formalin for patient specimens or is it for use only on the processor?Will you please tell us the cost and procedure for buffering? Can you estimate about how much tech time is spent with the system? Your response is appreciated. Thank you, Brandi Farris Boone Hospital Center Columbia, MO 65201 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ------------------------------ Message: 3 Date: Tue, 7 Mar 2006 11:27:09 -0700 From: "Elizabeth Chlipala" Subject: [Histonet] CD69, CD94 and Bax antibodies for mouse tissue To: Message-ID: <000001c64214$bf248aa0$c6d48a80@Chlipala> Content-Type: text/plain; charset="us-ascii" Hello everyone I was wondering what was a good source for the following antibodies for mouse tissue. Any suggestions would be helpful. thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 ------------------------------ Message: 4 Date: Tue, 7 Mar 2006 19:34:38 +0100 From: "Gudrun Lang" Subject: [Histonet] Hall or fouchet stain To: "Histonetliste \(Histonetliste\)" Message-ID: <002f01c64215$ccb52d90$eeeea8c0@SERVER01> Content-Type: text/plain; charset="us-ascii" Can somebody help me with the shelflive of this reagens'? 25% trichloracetic acid Fouchet reagens: 100ml 25% trichloracetic acid and 10ml 10%ferrichlorid Thank you Gudrun Lang Akh Linz Austria ------------------------------ Message: 5 Date: Tue, 7 Mar 2006 13:44:57 -0500 From: "Jacqui Detmar" Subject: RE: [Histonet] CD69, CD94 and Bax antibodies for mouse tissue To: "Elizabeth Chlipala" , Message-ID: Content-Type: text/plain; charset="iso-8859-1" I have used the BaxNT antibody from Upstate (06-499)for both IHC and Western blotting with good results...wildtype tissues (placentae, mainly) are positive and the knockout tissues are negative. Epitomics has come out with a new rabbit monoclonal antibody for Bax which I am tempted to try, but have not yet done so. Jacqui -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Elizabeth Chlipala Sent: Tuesday, March 07, 2006 1:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD69, CD94 and Bax antibodies for mouse tissue Hello everyone I was wondering what was a good source for the following antibodies for mouse tissue. Any suggestions would be helpful. thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Tue, 7 Mar 2006 14:38:56 -0500 From: "UnJa L. Hayes" Subject: [Histonet] Vital nerve tissue stain (in vivo) To: "histonet@lists.utsouthwestern.edu" Message-ID: <1141760336.440de150b876b@mail-www2.oit.umass.edu> Content-Type: text/plain; charset=ISO-8859-1 Dear Histonet, Does anyone know of a stain I could use in vivo that would help visualize nerves so as to make them easier to locate? I need to cut specific nerves in the lower abdominal area of a small rodent. However, I'm having difficulty finding the nerves (or all of the branches of the nerves). I've searched the web, but couldn't find any simple stains that I could use in vivo . Any assistance would be greatly appreciated!!!! u -- UnJa L. Hayes, Ph.D. Center for Neuroendocrine Studies University of Massachusetts Department of Psychology Tobin Hall Amherst, MA 01003 Off: (413) 545-5955 Lab: (413) 545-0526 Fax: (413) 545-0996 ------------------------------ Message: 7 Date: Tue, 07 Mar 2006 18:08:03 -0500 From: "Richard Cartun" Subject: [Histonet] DAKO AEC+ To: Message-ID: Content-Type: text/plain; charset=US-ASCII Is anyone having problems with DAKO's AEC+ chromogen (tiny dots everywhere). Thanks! Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax ------------------------------ Message: 8 Date: Wed, 8 Mar 2006 09:00:09 +0530 From: "Ravishankar Nagarajan" Subject: [Histonet] RE: Histonet Digest, Vol 28, Issue 11 To: Message-ID: <2533F91DF0FC3F48AE4919B3197765ED2E9619@GUR-PR-EXA1.Ranbaxy.com> Content-Type: text/plain; charset="iso-8859-1" Dear Group, Can anybody provide me the procedure for Wright Giemsa staining procedure for Bone marrow smears. We are performing the Wright's procedure here and we get inconsistent results here. Like the nuclei do not get stained. Could it be due to the fact that Wright's is only a cytoplasmic stain. Any suggestions would be welcome. I also include the procedure by which we do Wright's here. Wright's stain - 8 - 10 minutes Sorensen's buffer (pH - 7) - 8 -10 minutes Regards and Thanks, Dr.N>Ravishankar (i) The information contained in this e-mail message is intended only for the confidential use of the recipient(s) named above. This message is privileged and confidential. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. (ii) The sender confirms that Ranbaxy shall not be responsible if this email message is used for any indecent, unsolicited or illegal purposes, which are in violation of any existing laws and the same shall solely be the responsibility of the sender and that Ranbaxy shall at all times be indemnified of any civil and/ or criminal liabilities or consequences there. ------------------------------ Message: 9 Date: Wed, 08 Mar 2006 01:53:35 -0500 From: John Kiernan Subject: Re: [Histonet] Vital nerve tissue stain (in vivo) To: "UnJa L. Hayes" Cc: "histonet@lists.utsouthwestern.edu" Message-ID: <440E7F6F.B2E54CED@uwo.ca> Content-Type: text/plain; charset=us-ascii Yes. Methylene blue. There is a large body of literature dating from Paul Ehrlich (1880s) to the 1990s and perhaps later. Vital staining with methylene blue should reveal the nerves in a surgical setting, but you will need to dig in the literature for practical details. This is a method that needs intelligence rather than a "protocol." For my humble contribution see Histochemistry 40: 51-57 (1974). John Kiernan London, Canada ----------------------------- "UnJa L. Hayes" wrote: > > Dear Histonet, > > Does anyone know of a stain I could use in vivo that would help > visualize nerves so as to make them easier to locate? > > I need to cut specific nerves in the lower abdominal area of a small > rodent. However, I'm having difficulty finding the nerves (or all of > the branches of the nerves). I've searched the web, but couldn't find > any simple stains that I could use in vivo . > > Any assistance would be greatly appreciated!!!! > > u > > -- > UnJa L. Hayes, Ph.D. > Center for Neuroendocrine Studies > University of Massachusetts > Department of Psychology > Tobin Hall > Amherst, MA 01003 > Off: (413) 545-5955 > Lab: (413) 545-0526 > Fax: (413) 545-0996 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Tue, 7 Mar 2006 20:49:03 -0500 From: Eric Dye (ext 223) Subject: [Histonet] Histology Managers, Supervisors, and Bench Techs needed- Intervirewing and Hiring NOW To: Histonetters Message-ID: Content-Type: text/plain Fellow-Histonetters I'm presently on a search for my best client companies seeking HistoTech Supervisors and Histo Bench Techs. These are mostly fulltime permanent positions. Most of my Histo Techs positions are clinical, although I do have a few Temporary and Research Histo Tech positions as well. Here are some of my Hottest jobs: 1. Ohio (Full-time, Perm, Bench, 3rd Shift) 2. Ohio (Full-time, Perm, MANAGER) 3. Ohio (full-time, Perm,Bench, 3rd Shift) 4. Colorado (Full-Time, Perm, Bench, 3rd Shift) 5. Rhode Island (Full-time, Perm, Bench, 2nd Shift) 6. New Jersey (Full-time, Perm, Bench) 7. Massachusetts (Boston) (Part-time, Bench) 8. New York (Long Island) (Full-time, Perm, SUPERVISOR) 9. Illinois (Full-time, Perm, SUPERVISOR) 10. Virginia(South) (Full-time, Perm, Bench) 11. Virginia (Full-time, Perm, LEAD TECH) 12. West Virginia (Full-time, Perm, Bench) The clients are currently interviewing candidates - so if you are interested, please call me today at 1-800-466-9919 ext 223 Thank you, Eric Dye-Sr Allied Healthcare Recruiter 1-800-466-9919 ext 223 --------------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing 800 466 9919 ext 223 - voice 407 671 6075 - fax eric@ategra.com To Learn More About Ategra: http://www.ategra.com ---------------------------------------------------------------- Note: this message is intended for: Fellow-Histonetters at histonet@lists.utsouthwestern.edu ---------------------------------------------------------------- ---------------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. ---------------------------------------------------------------- ------------------------------ Message: 11 Date: Wed, 8 Mar 2006 17:28:19 +0530 From: "Arvind" Subject: [Histonet] cryo soft tissue To: Message-ID: <000801c642a7$979bce60$aa00a8c0@nbrc.res.in> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original can any one provide detailed protocol for cryosectioning of human fetus brain ageing 3 months prenatal to 01 day postnatal using cryotome thanks in advance Arvind Singh Pundir National Brain Research Centre Manesar Gurgaon, HARYANA INDIA arvind@nbrc.res.in ------------------------------ Message: 12 Date: Wed, 8 Mar 2006 04:49:34 -0800 (PST) From: cynthia haynes Subject: [Histonet] negative immunohistochemistry control To: Histonet@lists.utsouthwestern.edu Message-ID: <20060308124934.59087.qmail@web33011.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Good Morning everyone, I have a question about immuno staining. I've been away from this type of staining for a while and now I am doing them again on a regular basis. Why do you run a negative control with each run? Are you only suppose to put the normal serum on negative control only? I've forgotten; would someone please answer these questions for me. Thanks in advance. Cynthia Haynes H.T. ------------------------------ Message: 13 Date: Wed, 8 Mar 2006 08:23:21 -0500 From: "Kellar, Eric C" Subject: [Histonet] RE: Negative Immunohistochemistry controls To: histonet@lists.utsouthwestern.edu Message-ID: <6843061CE6B98E4B96590D4F299618F801583BAF@qdcws0117.us.qdx.com> Content-Type: text/plain; charset=iso-8859-1 Cynthia, Negative controls are used to check the presence of non-specific staining. An adequate (-) control consists of replacing the specific primary antibody by another antibody produced in the same species but with unrelated specificity. When using polyclonal antisera, a preimmune serum may be used. With monoclonal antibodies, normal mouse serum or non-immune mouse ascites is applied. They are used to evaluate the non-specific binding of that isotope to the cells. They are applied in the highest concentration that is used for the specific antibodies. In indirect or multi-step techniques the primary antibody may also be omitted to evaluate the non-specific binding of the other reagents. Eric C. Kellar Histology/Immunohistochemistry Quest Diagnostics, Inc Miami Good Morning everyone, I have a question about immuno staining. I've been away from this type of staining for a while and now I am doing them again on a regular basis. Why do you run a negative control with each run? Are you only suppose to put the normal serum on negative control only? I've forgotten; would someone please answer these questions for me. Thanks in advance. Cynthia Haynes H.T. ============================================================================== The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. ============================================================================== ------------------------------ Message: 14 Date: Wed, 8 Mar 2006 07:35:54 -0600 From: "Chris Pomajzl" Subject: Re: [Histonet] negative immunohistochemistry control To: "HISTONET" , "cynthia haynes" Message-ID: <002101c642b5$3d31b760$26fca8c0@CSP> Content-Type: text/plain; charset="iso-8859-1" There are several alternatives for negative controls, but a common one is simply to use the buffer rinse in place of the primary antibody, thereby removing the first link in the chain. Another way is to keep everything the same and run the IHC stain on negative tissue (no antigen present in the tissue). ----- Original Message ----- From: "cynthia haynes" To: Sent: Wednesday, March 08, 2006 6:49 AM Subject: [Histonet] negative immunohistochemistry control > Good Morning everyone, I have a question about immuno staining. I've > been away from this type of staining for a while and now I am doing > them again on a regular basis. Why do you run a negative control with > each run? Are you only suppose to put the normal serum on > negative control only? I've forgotten; would someone > please answer these questions for me. Thanks in > advance. > > Cynthia Haynes H.T. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Wed, 8 Mar 2006 08:48:58 -0500 From: "Kellar, Eric C" Subject: [Histonet] Correction RE: Negative Immunohistochemistry controls To: histonet@lists.utsouthwestern.edu Message-ID: <6843061CE6B98E4B96590D4F299618F801583BB0@qdcws0117.us.qdx.com> Content-Type: text/plain; charset=iso-8859-1 Cynthia, "They are used to evaluate the non-specific binding of that 'epitope' to the cells" I typed much too fast and neglected to proof read! Sorry about that! Eric C. Kellar Histology/Immunohistochemistry Quest Diagnostics, Inc. Miami ============================================================================== The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. ============================================================================== ------------------------------ Message: 16 Date: Wed, 8 Mar 2006 05:51:35 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] negative immunohistochemistry control To: cynthia haynes , Histonet@lists.utsouthwestern.edu Message-ID: <20060308135135.78870.qmail@web61222.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Cynthia: The idea of the negative control is to try to determine if any reaction detected in the case is due to specific or unspecific binding. You don't need to run a negative slide for each antibody you are going to test on the case, you only need a negative slide per case, no matter how many antibodies you run. The only requirement is that the negative slide has to come from the same tissue tested. What I used to do was to add buffer instead of the primary Ab and all the other reagents the same as in the slides run for specific Abs. You could also add non-immune globuline instead of the antibody but in this case you would need 1 negative control for each type Ab (1 negative for monoclonal Abs and 1 for polyclonal Abs). I hope this will help you. Ren? J. cynthia haynes wrote: Good Morning everyone, I have a question about immuno staining. I've been away from this type of staining for a while and now I am doing them again on a regular basis. Why do you run a negative control with each run? Are you only suppose to put the normal serum on negative control only? I've forgotten; would someone please answer these questions for me. Thanks in advance. Cynthia Haynes H.T. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Mail Bring photos to life! New PhotoMail makes sharing a breeze. ------------------------------ Message: 17 Date: Wed, 8 Mar 2006 14:08:29 -0000 From: "Edwards, R.E." Subject: RE: [Histonet] negative immuno histochemistry control To: "cynthia haynes" , Message-ID: Content-Type: text/plain; charset="iso-8859-1" For polyclonal antibodies use species specific(rabbit,goatetcetc) normal serum, or pre-immune if available: for mouse monoclonals use the specific isotypic e.g. IgG2a; simply omitting the primary antibody or replacing it with diluent alone is no control. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of cynthia haynes Sent: 08 March 2006 12:50 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] negative immunohistochemistry control Good Morning everyone, I have a question about immuno staining. I've been away from this type of staining for a while and now I am doing them again on a regular basis. Why do you run a negative control with each run? Are you only suppose to put the normal serum on negative control only? I've forgotten; would someone please answer these questions for me. Thanks in advance. Cynthia Haynes H.T. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Wed, 8 Mar 2006 09:12:47 -0500 From: "Martha Ward" Subject: [Histonet] Outsourcing transcription To: Message-ID: <61135F0455D33347B5AAE209B903A30412C89490@EXCHVS2.medctr.ad.wfubmc.edu> Content-Type: text/plain; charset="us-ascii" I am posting this for our manager. Our administration would like us to explore outsourcing our Pathology transcription and we would like to hear from anyone who has done this: vendor, cost, turnaround times. We currently use CoPath and the vendor would have to be compatible with this. Thanks in advance for your help. Martha Ward Wake Forest University Baptist Medical Center Dept. of Pathology Winston-Salem, NC 27157 ------------------------------ Message: 19 Date: Wed, 8 Mar 2006 08:21:09 -0600 From: "Chris Pomajzl" Subject: Re: [Histonet] negative immunohistochemistry control To: "HISTONET" , "Rene J Buesa" Message-ID: <003b01c642bb$8c1baf60$26fca8c0@CSP> Content-Type: text/plain; charset="iso-8859-1" I don't understand the idea of only one negative control per case, as opposed to each antibody. How does one evaluate non-specific staining of antibodies that do not have a negative control? What if non-specific staining is inherent to a certain antibody? For example, the new CAP question regarding staining of endogenous biotin, particularly in kidney and liver - if you do not run a negative control for that specific antibody, how do you know if there is non-specific staining? ----- Original Message ----- From: "Rene J Buesa" To: "cynthia haynes" ; Sent: Wednesday, March 08, 2006 7:51 AM Subject: Re: [Histonet] negative immunohistochemistry control > Cynthia: > The idea of the negative control is to try to determine if any > reaction detected in the case is due to specific or unspecific binding. > You don't need to run a negative slide for each antibody you are > going to test on the case, you only need a negative slide per case, no matter how many antibodies you run. The only requirement is that the negative slide has to come from the same tissue tested. > > What I used to do was to add buffer instead of the primary Ab and > all the other reagents the same as in the slides run for specific Abs. You could also add non-immune globuline instead of the antibody but in this case you would need 1 negative control for each type Ab (1 negative for monoclonal Abs and 1 for polyclonal Abs). > I hope this will help you. > Ren? J. > > cynthia haynes wrote: > Good Morning everyone, I have a question about immuno staining. I've > been away from this type of staining for a while and now I am doing > them again on a regular basis. Why do you run a negative control with > each run? Are you only suppose to put the normal serum on > negative control only? I've forgotten; would someone > please answer these questions for me. Thanks in > advance. > > Cynthia Haynes H.T. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Yahoo! Mail > Bring photos to life! New PhotoMail makes sharing a breeze. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Wed, 8 Mar 2006 08:24:30 -0600 From: "Dawson, Glen" Subject: RE: [Histonet] negative immuno histochemistry control To: "Edwards, R.E." , cynthia haynes , Histonet@lists.utsouthwestern.edu Message-ID: <84A42E6F38BE8A45AAB03628C8C80D1204C2C6@dynams.dynacaremilwaukee.com> Content-Type: text/plain; charset="iso-8859-1" Although this is a good point, I don't think this is optimal unless the normal serum or pre-immune that is used for the negative is from the exact same animal/supernatant that the antibody is drawn from with the antibody seperated out. Throwing in something "else" is bringing another factor into the equation. How is a person to know that the "exceptable" negative was collected correctly or that it is not contaminated. That being said, I myself do the above to remain CAP compliant. I used to do the antibody diluent for whichever antibody I was using for the negative control & I still think it's the most logical thing to do. By using diluent as the negative & keeping all other steps/reagents the same as the antibody you are running, you are ensuring that it is, indeed, something in the concentrated antibody that is causing staining not seen on the negative. Just My Opinion, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Edwards, R.E. Sent: Wednesday, March 08, 2006 8:08 AM To: cynthia haynes; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] negative immuno histochemistry control For polyclonal antibodies use species specific(rabbit,goatetcetc) normal serum, or pre-immune if available: for mouse monoclonals use the specific isotypic e.g. IgG2a; simply omitting the primary antibody or replacing it with diluent alone is no control. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of cynthia haynes Sent: 08 March 2006 12:50 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] negative immunohistochemistry control Good Morning everyone, I have a question about immuno staining. I've been away from this type of staining for a while and now I am doing them again on a regular basis. Why do you run a negative control with each run? Are you only suppose to put the normal serum on negative control only? I've forgotten; would someone please answer these questions for me. Thanks in advance. Cynthia Haynes H.T. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Wed, 8 Mar 2006 08:30:45 -0600 From: "Sebree Linda A." Subject: RE: [Histonet] negative immunohistochemistry control To: "Chris Pomajzl" , "HISTONET" , "Rene J Buesa" Message-ID: Content-Type: text/plain; charset="iso-8859-1" CAP states that for panels, running one negative control using the harshest protocol suffices. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chris Pomajzl Sent: Wednesday, March 08, 2006 8:21 AM To: HISTONET; Rene J Buesa Subject: Re: [Histonet] negative immunohistochemistry control I don't understand the idea of only one negative control per case, as opposed to each antibody. How does one evaluate non-specific staining of antibodies that do not have a negative control? What if non-specific staining is inherent to a certain antibody? For example, the new CAP question regarding staining of endogenous biotin, particularly in kidney and liver - if you do not run a negative control for that specific antibody, how do you know if there is non-specific staining? ----- Original Message ----- From: "Rene J Buesa" To: "cynthia haynes" ; Sent: Wednesday, March 08, 2006 7:51 AM Subject: Re: [Histonet] negative immunohistochemistry control > Cynthia: > The idea of the negative control is to try to determine if any > reaction detected in the case is due to specific or unspecific binding. > You don't need to run a negative slide for each antibody you are > going to test on the case, you only need a negative slide per case, no matter how many antibodies you run. The only requirement is that the negative slide has to come from the same tissue tested. > > What I used to do was to add buffer instead of the primary Ab and > all the other reagents the same as in the slides run for specific Abs. You could also add non-immune globuline instead of the antibody but in this case you would need 1 negative control for each type Ab (1 negative for monoclonal Abs and 1 for polyclonal Abs). > I hope this will help you. > Ren? J. > > cynthia haynes wrote: > Good Morning everyone, I have a question about immuno staining. I've > been away from this type of staining for a while and now I am doing > them again on a regular basis. Why do you run a negative control with > each run? Are you only suppose to put the normal serum on > negative control only? I've forgotten; would someone > please answer these questions for me. Thanks in > advance. > > Cynthia Haynes H.T. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Yahoo! Mail > Bring photos to life! New PhotoMail makes sharing a breeze. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 22 Date: Wed, 8 Mar 2006 09:34:41 -0500 From: "Weems, Joyce" Subject: [Histonet] FW: STATLINE -- Special Report: CMS Announces MUE Proposal Will Be Withdrawn To: "Histonet" Message-ID: <83AACDB0810528418AA106F9AE9B7F7E01305A9B@sjhaexc02.sjha.org> Content-Type: text/plain; charset="utf-8" Good news for now! STATLINE March 6, 2006 * ?? 2006 College of American Pathologists Special Report CMS Announces MUE Proposal Will Be Withdrawn MUE Proposal no Longer Scheduled to be Implemented in July At a meeting today of the Physician Payment Advisory Commission (PPAC), CMS officials said that the current Medically Unbelievable Edits (MUE) proposal is no longer scheduled to be implemented in July as previously planned. The proposal will be revised and re-submitted for public review and comment. This announcement followed concerns raised by the Chairman of the Physician Payment Advisory Commission Dr. Ronald Castellanos who stated that the current edits could have significant negative impacts on patient care. Testifying before the Commission was Dr. William Rogers, who stated that he had learned about the problems with the current MUE proposal from the College of American Pathologists in a meeting last week. He had discussed these concerns with the MUE Project manager at CMS. The PPAC recommended "the CMS withdraw the proposal to create a list of MUEs and resubmit through the normal rulemaking process and to work closely with the medical community in this effort." The motion was unanimously accepted. The CMS decision to withdraw the MUE proposal comes in direct response to an intense lobbying campaign by a CAP-led coalition of national and state pathology societies. The AMA, numerous medical specialty societies, and the American Clinical Laboratory Association (ACLA) also strongly advocated for the withdrawal of the MUE proposal. The College and its coalition partners have pressed CMS to withdraw the MUE proposal and have argued that the agency should be required to utilize the formal rulemaking process to develop sweeping unit of service limits on all pathology and clinical laboratory services. The College has also asked the agency to provide the rationale and methodology utilized to develop the MUE proposal. We are awaiting a written notification from the contractor withdrawing the proposal. CAP will continue to keep members informed on further details of CMS's plans in this area. _____ Information About This Service The CAP member mailing list is closed and confidential. Its purpose is to distribute important news and information to CAP members. Individual members are not able to post messages to the list. Visit the CAP Web site for additional news and information. If you have comments or questions regarding this mailing list, contact John Carter at jcarter@cap.org. To remove yourself from this list, go to http://leda.cap.org/UM/U.asp?B1.98.9473.85954 and you will be removed immediately. Thank you. ?? 2006, College of American Pathologists 325 Waukegan Road Northfield, IL 60093 Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. ------------------------------ Message: 23 Date: Wed, 8 Mar 2006 08:49:23 -0600 From: "Jackie M O'Connor" Subject: Re: [Histonet] negative immunohistochemistry control To: "Chris Pomajzl" Cc: HISTONET , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" I'm not in a clinical setting - but I use peptide inhibition as a negative control for each antibody. JO'C "Chris Pomajzl" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/08/2006 08:21 AM To: "HISTONET" , "Rene J Buesa" cc: (bcc: Jackie M O'Connor/LAKE/GPRD/ABBOTT) Subject: Re: [Histonet] negative immunohistochemistry control I don't understand the idea of only one negative control per case, as opposed to each antibody. How does one evaluate non-specific staining of antibodies that do not have a negative control? What if non-specific staining is inherent to a certain antibody? For example, the new CAP question regarding staining of endogenous biotin, particularly in kidney and liver - if you do not run a negative control for that specific antibody, how do you know if there is non-specific staining? ----- Original Message ----- From: "Rene J Buesa" To: "cynthia haynes" ; Sent: Wednesday, March 08, 2006 7:51 AM Subject: Re: [Histonet] negative immunohistochemistry control > Cynthia: > The idea of the negative control is to try to determine if any reaction detected in the case is due to specific or unspecific binding. > You don't need to run a negative slide for each antibody you are > going to test on the case, you only need a negative slide per case, no matter how many antibodies you run. The only requirement is that the negative slide has to come from the same tissue tested. > > What I used to do was to add buffer instead of the primary Ab and > all the other reagents the same as in the slides run for specific Abs. You could also add non-immune globuline instead of the antibody but in this case you would need 1 negative control for each type Ab (1 negative for monoclonal Abs and 1 for polyclonal Abs). > I hope this will help you. > Ren? J. > > cynthia haynes wrote: > Good Morning everyone, I have a question about immuno staining. I've > been away from this type of staining for a while and now I am doing > them again on a regular basis. Why do you run a negative control with > each run? Are you only suppose to put the normal serum on > negative control only? I've forgotten; would someone > please answer these questions for me. Thanks in > advance. > > Cynthia Haynes H.T. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Yahoo! Mail > Bring photos to life! New PhotoMail makes sharing a breeze. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 24 Date: Wed, 8 Mar 2006 07:19:00 -0800 From: Maria Mejia Subject: [Histonet] myelin stain for 40um cryostat sections To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed Could someone please recommend a good myelin stain protocol for 40um cryostat primate brain sections (both for fixed and fresh frozen sections? 10% NBF has been used on the fixed sections. I would greatly appreciate any information you could provide & thank you in advance. Yours Maria Bartola Mejia University California San Francisco (UCSF) Department of Neurosurgery San Francisco, CA 94103 Email: mbmphoto@gmail.com ------------------------------ Message: 25 Date: Wed, 8 Mar 2006 09:27:37 -0600 From: "Ford Royer" Subject: RE: [Histonet] RE: Histonet Digest, Vol 28, Issue 11 To: Message-ID: <004201c642c4$d4da7e30$6f01a80a@fords> Content-Type: text/plain; charset="us-ascii" Are you hand staining or using an automated stainer? Back when I did BM smears, we found that they had to be run through the stainer 2 or 3 times due to the thickness of the smear. This did work well however; and we didn't have any problems with nuclear staining or "over staining". ~ Ford Ford M. Royer, MT(ASCP) Minnesota Medical Specialists Minneapolis, MN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ravishankar Nagarajan Sent: Tuesday, March 07, 2006 9:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet Digest, Vol 28, Issue 11 Dear Group, Can anybody provide me the procedure for Wright Giemsa staining procedure for Bone marrow smears. We are performing the Wright's procedure here and we get inconsistent results here. Like the nuclei do not get stained. Could it be due to the fact that Wright's is only a cytoplasmic stain. Any suggestions would be welcome. I also include the procedure by which we do Wright's here. Wright's stain - 8 - 10 minutes Sorensen's buffer (pH - 7) - 8 -10 minutes Regards and Thanks, Dr.N>Ravishankar (i) The information contained in this e-mail message is intended only for the confidential use of the recipient(s) named above. This message is privileged and confidential. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. (ii) The sender confirms that Ranbaxy shall not be responsible if this email message is used for any indecent, unsolicited or illegal purposes, which are in violation of any existing laws and the same shall solely be the responsibility of the sender and that Ranbaxy shall at all times be indemnified of any civil and/ or criminal liabilities or consequences there. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 26 Date: Wed, 8 Mar 2006 15:35:53 -0000 From: Kemlo Rogerson Subject: RE: [Histonet] myelin stain for 40um cryostat sections To: "'Maria Mejia'" , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain Myelin can be stained with Sudan 3 and iron haematoxylin. Cut frozen (10 U) sections and chromate in 2.5% potassium dichromate for 2 to 4 days, then wash. 5ml fresh 1% aqueous haematoxylin and 5 ml 4% iron alum fresh in a covered dish. Put your frozen sections in for 45 min at 60 degrees C. Agitate gently. Wash in water. Decolorise in 0.5% iron alum for 1 hr with agitation. Wash in water Treat with 1% borax/ 2.5% potassium ferricyanide solution for 10 min with agitation. Wash in water 6mls stock Sudan 2 (CI 12140) saturated in 99% isopropanol and dilute with 4 ml water, let stand 7 to 8 min and filter. Stain sections 10 min. wash in water Float onto slides, drain and mount in aqueous mountant. This is from Lillie and I used it decades ago with success; but the old ways are usually the best. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Maria Mejia [mailto:mbmphoto@gmail.com] Sent: Wednesday, March 08, 2006 3:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] myelin stain for 40um cryostat sections Could someone please recommend a good myelin stain protocol for 40um cryostat primate brain sections (both for fixed and fresh frozen sections? 10% NBF has been used on the fixed sections. I would greatly appreciate any information you could provide & thank you in advance. Yours Maria Bartola Mejia University California San Francisco (UCSF) Department of Neurosurgery San Francisco, CA 94103 Email: mbmphoto@gmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 28, Issue 12 **************************************** From jotahal <@t> ftvs.cuni.cz Thu Mar 9 02:24:32 2006 From: jotahal <@t> ftvs.cuni.cz (=?iso-8859-2?Q?Jakub_Ot=E1hal?=) Date: Thu Mar 9 02:25:07 2006 Subject: [Histonet] Where to purchase India Ink Message-ID: <000401c64352$e7cb2fb0$0601a8c0@d333jotahal> Hi histoneters, Does anybody know where to purchase India Ink for easy vizualization of cerebral vascularization? Thanks for any suggestion jakub -------------------------------------------------------------------------- Jakub Otahal MD,PhD Department of Developemental Epileptology Institute of Physiology Czech Academy of Sciences Videnska 1083 142 20 Prague 4 Czech Republic tel: +420 241062495 jotahal@ftvs.cuni.cz ------------------------------------------------------------------------- From fadiafandi <@t> mac.com Thu Mar 9 07:04:28 2006 From: fadiafandi <@t> mac.com (fadiafandi@mac.com) Date: Thu Mar 9 07:04:35 2006 Subject: [Histonet] Re: Negative IHC controls In-Reply-To: <200603081806.k28I6TM0010111@mac.com> References: <200603081806.k28I6TM0010111@mac.com> Message-ID: Dear colleagues, The CAP checklist has a good discussion of negative IHC controls. One cant expand by reading the quoted references in order to understand the background behind negative controls. Many of the opinions mentioned in this discussion are also excellent and make scientific sense. Briefly, a negative control is used to ensure that any signal on the patient's tissue that *contains* the primary antibody is not a false- positive signal. That false-positive signal could be secondary many factors including internal tissue properties (i.e., endogenous biotin in the case of ABC detection system, inadequate fixation, etc.), unusually high antibody concentration, excessive pretreatment, prolonged incubation with the primary antibody, inadequate wash steps, lifting of tissue section, etc. If a case has 5 antibodies with 2 different detection systems and 3 different pretreatment methods, in order to fully address the issue of negative control, one should include negative control sections from the same patient by eliminating each one of the above-mentioned parameters, one at a time, which means running multiple negative controls for the same tissue. In real life, however, that doesn't seem to be necessary. Replacing the primary antibody with a non-specific IgG would suffice. And that is in compliance with the CAP requirements. For the sake of standardization, a few manufacturers are in the process of developing multi-pellet controls for all the different steps in the process, which has the potential of achieving a very high level of specificity in terms of eliminating any possibility of false-positive signal. In our laboratory, we have been running a single patient's section with non-specific IgG serum per tissue block. Best regards, Hadi Yaziji, M.D. Ancillary Pathways, LLC. From oshel1pe <@t> cmich.edu Thu Mar 9 07:28:04 2006 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Thu Mar 9 07:28:15 2006 Subject: [Histonet] Reproductive Toxins In-Reply-To: <5D2189E74151CC42BEC02906BA8996322B919B@exchsrv01.barrynet.barry.edu> References: <5D2189E74151CC42BEC02906BA8996322B919B@exchsrv01.barrynet.barry.edu> Message-ID: I would add to this list phalloidin (and phallocidin), a mycotoxin that binds to filamentous actin (f actin). Commonly conjugated to a fluorochrome (e.g. Rhodamine, FITC, Texas Red) to light up the actin filaments in cytoskeletons. Phil > It is true that few plant or animal toxins are uses in histology labs. >Cottonmouth mocassin venom is used to prepare the substrate for >demonstrating phospholipase B. Fluoroescent-labelled banded krait (the "dust >snake" in RIKKI-TIKKI-TAVI) is used to demonstrate nicotinic acetylcholine >receptors. Ouabain (from an African tree) is used as a control in >demonstrating sodium/potassium ATPase. > Nevertheless, many things in a histology labe are toxic. Since MSDS's >tell you that everything (including table sugar) is toxic, it is hard find >the real toxicity data in them. (Hint: it is usually buried 2/3 of the way >down the second page as LD50 (how much has a 50% chance of killing you) or >TWA (the maximum concentration you can safely have in the air around you). >Often the MSDS wont't give you either piece of information. > I look up the toxicity of dyes in Dapson's HAZARDOUS MATERIALS IN THE >HISTOPATHOLOGY LABORATORY. I look up the toxicity of everything else in >Lewis's HAZARDOUS CHEMICALS DESK REFERENCE. The former is an indispensible >reference; the latter is very nice to have. > >Allen A. Smith, Ph.D. >Professor of Anatomy >Barry University School of Graduate Medical Sciences > Podiatric Medicine and Surgery >Miami Shores, Florida 33161 > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela >Bitting >Sent: Monday, March 06, 2006 4:08 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Reproductive Toxins > >Does anyone know how to tell if something in the Histo lab is a reproductive >toxin or acute toxin? Some of the MSDS sheets are no help at all. Anyone >else having this problem? > >Angela Bitting, HT(ASCP) >Technical Specialist, Histology >Geisinger Medical Center >100 N Academy Ave. MC 23-00 >Danville, PA 17822 >phone 570-214-9634 >fax 570-271-5916 -- Philip Oshel Microscopy Facility Supervisor Department of Biology Central Michigan University 024C Brooks Hall Mt. Pleasant, MI 48859 (989) 774-3576 From KevinMcGovern <@t> catholichealth.net Thu Mar 9 08:08:26 2006 From: KevinMcGovern <@t> catholichealth.net (McGovern, Kevin) Date: Thu Mar 9 08:08:48 2006 Subject: [Histonet] Where to purchase India Ink Message-ID: Jakub, One of the best places would be a graphic arts store such as Michael's here in the U.S., or Wal-Mart. If you have any kind of artist's supply store there, you should find India ink. Good luck! Kevin -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jakub Ot?hal Sent: Thursday, March 09, 2006 2:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Where to purchase India Ink Hi histoneters, Does anybody know where to purchase India Ink for easy vizualization of cerebral vascularization? Thanks for any suggestion jakub -------------------------------------------------------------------------- Jakub Otahal MD,PhD Department of Developemental Epileptology Institute of Physiology Czech Academy of Sciences Videnska 1083 142 20 Prague 4 Czech Republic tel: +420 241062495 jotahal@ftvs.cuni.cz ------------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MSafron <@t> wilresearch.com Thu Mar 9 09:07:51 2006 From: MSafron <@t> wilresearch.com (Mike Safron) Date: Thu Mar 9 09:10:49 2006 Subject: [Histonet] M Trichrome on Plastic Embedded Kidneys Message-ID: Kathy, If you need a copy of a protocol for trichrome staining on methacrylate embedded kidneys email me with a fax or an email for a PDF file. Michael Safron A.S.,HT(ASCP) Manager, Histology WIL Research Laboratories LLC 419-289-8700 ext: 3040 Ashland, Ohio 44805 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy.Johnston@CLS.ab.ca Sent: Monday, March 06, 2006 11:25 AM To: histonet@pathology.swmed.edu Subject: [Histonet] M Trichrome on Plastic Embedded Kidneys Does anyone out there do Masson Trichromes staining on Plastic embedded kidney sections? They don't seem to stain too badly with the M Trichrome that we use for our Paraffin sections, but I can't help wondering if there is a specific method that deals with the plastic residue surrounding (and possibly obscuring) the tissue. This is something new we're trying, so any info would be gratefully received! Thanks Kathy CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify the sender immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. From themagoos <@t> rushmore.com Thu Mar 9 09:16:17 2006 From: themagoos <@t> rushmore.com (Jason and Heather) Date: Thu Mar 9 09:16:40 2006 Subject: [Histonet] Microwave Processing Program Templates References: Message-ID: <001401c6438c$6d6dc270$102c9e43@magoo> I was asked to see if anyone in histoland has a microwave processing program template that they would share with us. We have two TBS Shurwaves and are trying to use them to the max. We have a program for smal biopsies(GI, needle Bx, etc.) and a program for medium tissue(appendix, gallbladder, etc.) We are looking for programs that are for fatty tissue(breast, colon,etc.) and decal. If you could help please email me back with your suggestions. Thank You, Jason McGough, HT(ASCP) Clinical Laboratory of the Black Hills 2805 5th St. Rapid City, SD 57701 605-343-2267 themagoos@rushmore.com From JWEEMS <@t> sjha.org Thu Mar 9 09:21:56 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Thu Mar 9 09:21:49 2006 Subject: [Histonet] Where to purchase India Ink Message-ID: <83AACDB0810528418AA106F9AE9B7F7E01305AC0@sjhaexc02.sjha.org> Have you looked on line? We get ours from Dick Blick. There is a store here in Atlanta, but they have a web site - www.dickblick.com We have found that Higgins works better than their other, cheaper brands. Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 404-851-7831 - fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jakub Ot?hal Sent: Thursday, March 09, 2006 3:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Where to purchase India Ink Hi histoneters, Does anybody know where to purchase India Ink for easy vizualization of cerebral vascularization? Thanks for any suggestion jakub -------------------------------------------------------------------------- Jakub Otahal MD,PhD Department of Developemental Epileptology Institute of Physiology Czech Academy of Sciences Videnska 1083 142 20 Prague 4 Czech Republic tel: +420 241062495 jotahal@ftvs.cuni.cz ------------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From jreichensperger <@t> siumed.edu Thu Mar 9 09:35:47 2006 From: jreichensperger <@t> siumed.edu (Joel Reichensperger) Date: Thu Mar 9 09:35:58 2006 Subject: [Histonet] Problem with frozen sections for LCM Message-ID: <44104B53.50509@siumed.edu> Hi everyone, We are having an issue with ice artifact in our sections of fresh frozen mouse brain for use with LCM (laser capture microdissection). Currently, we remove the brain and immediately place it in liquid nitrogen and then store it at -80 until we are ready to section it. We cut 10um sections and once the sections are placed on the slide, they are put in a slide box that is kept on dry ice until all the slides are cut. At this point the box is placed back into the -80 until we are ready to stain them. Once ready to do the stain (Cresyl violet), I remove the slide and place it in ice cold acetone for 5 minutes to fix the sections. Should we be fixing the sections right after we cut them and before we put them in the freezer? The outer regions of the brain are the areas that look terrible, while the center morphology looks nearly perfect. Unfortunately, we are interested in the basal forebrain. Any help would be appreciated. Thanks -- Joel Reichensperger Researcher II Southern Illinois University School of Medicine (217)545-7292 Phone (217)545-0145 Fax From MSafron <@t> wilresearch.com Thu Mar 9 09:40:05 2006 From: MSafron <@t> wilresearch.com (Mike Safron) Date: Thu Mar 9 09:41:14 2006 Subject: [Histonet] FW: M Trichrome on Plastic Embedded Kidneys Message-ID: Email address might help.. msafron@wilresearch.com -----Original Message----- From: Mike Safron [mailto:MSafron@wilresearch.com] Sent: Thursday, March 09, 2006 10:08 AM To: 'histonet@pathology.swmed.edu' Subject: M Trichrome on Plastic Embedded Kidneys Kathy, If you need a copy of a protocol for trichrome staining on methacrylate embedded kidneys email me with a fax or an email for a PDF file. Michael Safron A.S.,HT(ASCP) Manager, Histology WIL Research Laboratories LLC 419-289-8700 ext: 3040 Ashland, Ohio 44805 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathy.Johnston@CLS.ab.ca Sent: Monday, March 06, 2006 11:25 AM To: histonet@pathology.swmed.edu Subject: [Histonet] M Trichrome on Plastic Embedded Kidneys Does anyone out there do Masson Trichromes staining on Plastic embedded kidney sections? They don't seem to stain too badly with the M Trichrome that we use for our Paraffin sections, but I can't help wondering if there is a specific method that deals with the plastic residue surrounding (and possibly obscuring) the tissue. This is something new we're trying, so any info would be gratefully received! Thanks Kathy CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify the sender immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. From jreichensperger <@t> siumed.edu Thu Mar 9 09:42:38 2006 From: jreichensperger <@t> siumed.edu (Joel Reichensperger) Date: Thu Mar 9 09:42:45 2006 Subject: [Histonet] IHC training Message-ID: <44104CEE.6030907@siumed.edu> Hi everyone, My boss wants to send me somewhere to learn more about IHC techniques. Does anyone know of such a place? Are there any conferences that offer IHC workshops? Thanks for your help. -- Joel Reichensperger Researcher II Southern Illinois University School of Medicine (217)545-7292 Phone (217)545-0145 Fax From dellav <@t> musc.edu Thu Mar 9 09:42:09 2006 From: dellav <@t> musc.edu (Vinnie Della Speranza) Date: Thu Mar 9 09:47:19 2006 Subject: [Histonet] trying to locate Stewart Chew from Curtin University Message-ID: I am trying to locate Stewart Chew from Curtin University School of Biomedical Sciences in Perth, Australia. if anyone has contact information other than snail mail address (I have that) that would enable more rapid contact, I would be most appreciative. email would be best. thanks Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, SC 29425 Ph: 843-792-6353 fax: 843-792-8974 From Allison_Scott <@t> hchd.tmc.edu Thu Mar 9 09:53:44 2006 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Thu Mar 9 09:53:51 2006 Subject: [Histonet] Cap check list question concerning microwaves Message-ID: <1872B4A455B7974391609AD8034C79FC066FFC@LBEXCH01.hchd.local> Hello to all in histoland. I just received my cap self checklist. There is a new section concerning microwaves. We very seldom use our microwave, and we have never had it checked for leaks. How are others handling the new questions? Thanks in advance. Allison Scott Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From jkiernan <@t> uwo.ca Thu Mar 9 10:07:18 2006 From: jkiernan <@t> uwo.ca (John A. Kiernan) Date: Thu Mar 9 10:07:52 2006 Subject: [Histonet] Where to purchase India Ink References: <000401c64352$e7cb2fb0$0601a8c0@d333jotahal> Message-ID: <441052B6.41326E4D@uwo.ca> From an art shop, but make sure it really is Indian ink, not just any black ink. (Indian ink is colloidal carbon in water with some sort of binding polymer like dextrin or a protein.) I've used a make called Pelikan for marking, things, injecting blood vessels (mixed with gelatin) and by IV injection to label phagocytic cells in rats (it circulates for a minute or two and is then nearly all lodged in the liver and spleen). There will be other makes too. A Google search for "pelikan indian ink" (in quotes) brings up 102 hits, including vendors and biological applications. Omit the quotes and there are 33000 hits. Change indian to india and there are 67000 hits. The stuff shouldn't be too hard to find! -- ------------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan[AT]uwo.ca http://publish.uwo.ca/~jkiernan/ http://instruct.uwo.ca/anatomy/530/index.htm _______________________________ Jakub Ot?hal wrote: > > Hi histoneters, > > Does anybody know where to purchase India Ink for easy vizualization of > cerebral vascularization? > > > > Thanks for any suggestion > > > > jakub > > > > -------------------------------------------------------------------------- > > Jakub Otahal MD,PhD > > Department of Developemental Epileptology > > Institute of Physiology > > Czech Academy of Sciences > > Videnska 1083 > > 142 20 Prague 4 > > Czech Republic > > tel: +420 241062495 > > jotahal@ftvs.cuni.cz > > ------------------------------------------------------------------------- > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Thu Mar 9 10:17:40 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Thu Mar 9 10:17:38 2006 Subject: [Histonet] Where to purchase India Ink Message-ID: Just to emphasise the point, there are things that come in bottles labelled Indian ink, that are not. I have a big expensive bottle to prove it! Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: John A. Kiernan [mailto:jkiernan@uwo.ca] Sent: 09 March 2006 16:07 To: Jakub Ot?hal; Histonet Subject: Re: [Histonet] Where to purchase India Ink >From an art shop, but make sure it really is Indian ink, not just any black ink. (Indian ink is colloidal carbon in water with some sort of binding polymer like dextrin or a protein.) I've used a make called Pelikan for marking, things, injecting blood vessels (mixed with gelatin) and by IV injection to label phagocytic cells in rats (it circulates for a minute or two and is then nearly all lodged in the liver and spleen). There will be other makes too. A Google search for "pelikan indian ink" (in quotes) brings up 102 hits, including vendors and biological applications. Omit the quotes and there are 33000 hits. Change indian to india and there are 67000 hits. The stuff shouldn't be too hard to find! -- ------------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan[AT]uwo.ca http://publish.uwo.ca/~jkiernan/ http://instruct.uwo.ca/anatomy/530/index.htm _______________________________ Jakub Ot?hal wrote: > > Hi histoneters, > > Does anybody know where to purchase India Ink for easy vizualization of > cerebral vascularization? > > > > Thanks for any suggestion > > > > jakub > > > > -------------------------------------------------------------------------- > > Jakub Otahal MD,PhD > > Department of Developemental Epileptology > > Institute of Physiology > > Czech Academy of Sciences > > Videnska 1083 > > 142 20 Prague 4 > > Czech Republic > > tel: +420 241062495 > > jotahal@ftvs.cuni.cz > > ------------------------------------------------------------------------- > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ploykasek <@t> phenopath.com Thu Mar 9 10:18:06 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Thu Mar 9 10:18:28 2006 Subject: [Histonet] Negative IHC control Message-ID: Just a few more thoughts on the negative control issue. Everyone has raised very good & valid points. For the CAP checklist, it is good to remember the intent behind the question. I think a vital piece of information, is to know your IHC 'system' - antibodies, detection system, pretreatment, etc... This involves both the pathologist and the technologist. Both should have enough knowledge & experience to determine specificity & sensitivity of all of the components of the system. If you are not familiar with your antibodies & how they look, all the possible negative controls won?t help much. It is important when bringing ?on-line? new antibodies to test them on many tissues- both tumor & normal that should show positivity & negativity. Determine what the specificity & sensitivity is in your lab. There are varying pre-analytical factors that can contribute to non-specific staining. If you are very familiar with your ?system?, than running multiple negative controls is not necessary. In the clinical setting, it is important not to waste a patient?s precious diagnostic biopsy by running every possible permutation of negative control ? I have seen this happen on more than one occasion. Just my thoughts on the issue. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From dellav <@t> MUSC.EDU Thu Mar 9 10:27:00 2006 From: dellav <@t> MUSC.EDU (Vinnie Della Speranza) Date: Thu Mar 9 10:32:32 2006 Subject: [Histonet] Cap check list question concerning microwaves Message-ID: your facility should have a radiation safety officer who could provide the leakage testing you need. in some facilities Biomedical Engineering performs the service. chances are this capability is already available in your facility. Vinnie Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, SC 29425 Ph: 843-792-6353 fax: 843-792-8974 >>> "Scott, Allison D" 03/09/06 10:53AM >>> Hello to all in histoland. I just received my cap self checklist. There is a new section concerning microwaves. We very seldom use our microwave, and we have never had it checked for leaks. How are others handling the new questions? Thanks in advance. Allison Scott Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cpomajzl <@t> cpllabs.com Thu Mar 9 10:57:46 2006 From: cpomajzl <@t> cpllabs.com (Chris Pomajzl) Date: Thu Mar 9 10:55:13 2006 Subject: [Histonet] Cap check list question concerning microwaves References: <1872B4A455B7974391609AD8034C79FC066FFC@LBEXCH01.hchd.local> Message-ID: <007f01c6439a$974c1c50$26fca8c0@CSP> I found a website that sells microwave leakage detectors. ( http://www.amprobe.com/cgi-bin/pdc/viewprod.cgi?pid=72&tid=2&type=hvac ) They cost about $35-$40. The CAP question asks that you record and document this information on an annual basis, so you might want to purchase one. I would imagine that an outside company would charge you out the wazzoo to perform this simple check. ----- Original Message ----- From: "Scott, Allison D" To: Sent: Thursday, March 09, 2006 9:53 AM Subject: [Histonet] Cap check list question concerning microwaves Hello to all in histoland. I just received my cap self checklist. There is a new section concerning microwaves. We very seldom use our microwave, and we have never had it checked for leaks. How are others handling the new questions? Thanks in advance. Allison Scott Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Mar 9 11:28:26 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 9 11:28:31 2006 Subject: [Histonet] Cap check list question concerning microwaves In-Reply-To: <1872B4A455B7974391609AD8034C79FC066FFC@LBEXCH01.hchd.local> Message-ID: <20060309172826.48696.qmail@web61222.mail.yahoo.com> Allison: Sometimes the X-Rays dept. personnel can check your MW oven for leakage. Ren? J. "Scott, Allison D" wrote: Hello to all in histoland. I just received my cap self checklist. There is a new section concerning microwaves. We very seldom use our microwave, and we have never had it checked for leaks. How are others handling the new questions? Thanks in advance. Allison Scott Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Mail Use Photomail to share photos without annoying attachments. From jkiernan <@t> uwo.ca Thu Mar 9 12:19:58 2006 From: jkiernan <@t> uwo.ca (John A. Kiernan) Date: Thu Mar 9 12:20:05 2006 Subject: [Histonet] Problem with frozen sections for LCM References: <44104B53.50509@siumed.edu> Message-ID: <441071CE.41C60A4B@uwo.ca> The cause of your trouble is putting the specimen into liquid nitrogen, which forms an insulating blanket of nitrogen gas around the immersed object. Try putting your hand in the liquid N2, and see how long it takes to freeze it solid. :) The archives at www.histosearch.com contain many Histonet hints about how to freeze tissue. Yours is a question that is frequently asked. -- ------------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan[AT]uwo.ca http://publish.uwo.ca/~jkiernan/ http://instruct.uwo.ca/anatomy/530/index.htm _______________________________ Joel Reichensperger wrote: > > Hi everyone, > > We are having an issue with ice artifact in our sections of fresh frozen > mouse brain for use with LCM (laser capture microdissection). Currently, > we remove the brain and immediately place it in liquid nitrogen and then > store it at -80 until we are ready to section it. We cut 10um sections > and once the sections are placed on the slide, they are put in a slide > box that is kept on dry ice until all the slides are cut. At this point > the box is placed back into the -80 until we are ready to stain them. > Once ready to do the stain (Cresyl violet), I remove the slide and place > it in ice cold acetone for 5 minutes to fix the sections. Should we be > fixing the sections right after we cut them and before we put them in > the freezer? The outer regions of the brain are the areas that look > terrible, while the center morphology looks nearly perfect. > Unfortunately, we are interested in the basal forebrain. Any help would > be appreciated. > > Thanks > > -- > Joel Reichensperger > Researcher II > Southern Illinois University School of Medicine > (217)545-7292 Phone > (217)545-0145 Fax > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ja.mitchell <@t> hosp.wisc.edu Thu Mar 9 12:29:56 2006 From: ja.mitchell <@t> hosp.wisc.edu (Mitchell Jean A.) Date: Thu Mar 9 12:30:02 2006 Subject: [Histonet] NSH 2006 Awards/Scholarships Message-ID: <583D3E9A1E843445BD54E461D1A2F6F3192C1925@uwhis-xchng2.hosp.wisc.edu> A wonderful financial benefit of your NSH membership is the Annual Awards & Scholarship program. This week NSH members should be receiving a fantastic brochure on the 2006 Award/Scholarship offerings that NSH members and students in approved schools of Histotechnology can apply or be nominated for. The awards listing and forms are also available on the NSH website www.nsh.org Please review the awards and their criteria and consider applying for an award(s) that you are eligible for. Or nominate a colleague that is deserving of recognition of their achievements and contributions to the profession of histotechnology. All submissions and nominations must be postmarked by May 1, 2006. Recipients of the 2006 NSH Awards & Scholarships will be honored at the 32nd Annual Symposium/Convention, September 8-13, 2006 in Phoenix. Thank-you and Good Luck! Jean Mitchell Chair, 2006 NSH Awards Committee From immuno33 <@t> yahoo.com Thu Mar 9 12:47:58 2006 From: immuno33 <@t> yahoo.com (immuno33) Date: Thu Mar 9 12:48:02 2006 Subject: [Histonet] Billing charges for Microarray analysis Message-ID: <20060309184759.99834.qmail@web51715.mail.yahoo.com> Hi all! Does anyone here routinely charge for services related to Microarray analysis? I am starting to do some analysis on 444 core arrays and trying to figure out how much to charge the client and what everyone else is doing. Thanks for any help! John __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From LuckG <@t> empirehealth.org Thu Mar 9 15:44:50 2006 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Thu Mar 9 15:44:55 2006 Subject: [Histonet] Cap check list question concerning microwaves Message-ID: Allison, An engineer from our physical plant did it for me. Turns out they had an older instrument in house that had to be used frequently when microwave use and distribution with-in the hospital was a newer issue. Worked fine for us. Try that approach. Good luck, Greg Greg Luck, BS, HT(ASCP) Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 Phone 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmedicalcenter.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Sent: Thursday, March 09, 2006 9:28 AM To: Scott, Allison D; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cap check list question concerning microwaves Allison: Sometimes the X-Rays dept. personnel can check your MW oven for leakage. Ren? J. "Scott, Allison D" wrote: Hello to all in histoland. I just received my cap self checklist. There is a new section concerning microwaves. We very seldom use our microwave, and we have never had it checked for leaks. How are others handling the new questions? Thanks in advance. Allison Scott Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Mail Use Photomail to share photos without annoying attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From srwilkes <@t> hotmail.com Thu Mar 9 22:29:01 2006 From: srwilkes <@t> hotmail.com (Steven Wilkes) Date: Thu Mar 9 22:29:07 2006 Subject: [Histonet] Histology position in DC area Message-ID: I am currently seeking a full or part time histology position in the Washington DC area. I have 2 years of full time Histology experience, as well as 4 summers while in high school/college. Are they any openings available? Thanks Steven srwilkes@hotmail.com From Joseph.Garvin <@t> NUIGALWAY.IE Fri Mar 10 04:53:54 2006 From: Joseph.Garvin <@t> NUIGALWAY.IE (0104684s) Date: Fri Mar 10 04:57:23 2006 Subject: [Histonet] cryostat mounting Message-ID: <441156C4@bodkin.nuigalway.ie> Has anyone any experience of mounting frozen tissue sections onto the chuck in a cryostat using water as opposed to OCT From jqb7 <@t> cdc.gov Fri Mar 10 05:16:01 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Fri Mar 10 05:17:53 2006 Subject: [Histonet] Leica ST5020 Multistainer and coverlsipper Message-ID: hi everyone: Not long ago I sent out a request regarding the Leica Autostainer and transfer station/coverslipping unit. I actually meant to ask about their Multistainer unit. Please email me with any feedback you care to share. Thanks, Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov From rjbuesa <@t> yahoo.com Fri Mar 10 07:50:13 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 10 07:50:15 2006 Subject: Fwd: Re: [Histonet] cryostat mounting Message-ID: <20060310135013.99610.qmail@web61217.mail.yahoo.com> [As shared response] Rene J Buesa wrote: Date: Fri, 10 Mar 2006 05:47:20 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] cryostat mounting To: 0104684s Hi Joseph: Distilled water is what I always used when doing direct immunofluorescence (DIF) in skin biopsies, either punch or shave. I did it in the following way: 1- you set the holder in the thermal absorption bar in the side of the cryostat 2- you should have at least 1 pair of fine tipped forceps also inside the cryostat so they are cold enough 3- you hold a dropper with distilled water with your left hand and with your right hand you hold the skin biopsy with the fine tipped forceps 4- you add a small amount of water (about 0.5 mL) on the chuck and as the water begins to solidify you set the skin sample over that drop of water that is starting to become ice 5- once the skin is in place on the water/ice interface, you keep adding water and building a small and round ice block over it 6- the ones I enjoyed most doing were those around the skin shave biopsies.It was like building a cocoon around them! 7- once the skin biopsy is totally covered with the ice, you take it in the chuck to the cryostat and start sectioning. The sections (over a very cold permanent or steel blade) slide beautifully and when picked up with the slide (always much wamer) immediately melts out leaving the skin section on it. I personally prefer cutting skin biopsis for DIF when they are embedded in water, rather than in OCT ("too messy" for my preference). Hope this description will help you! Ren? J. 0104684s wrote: Has anyone any experience of mounting frozen tissue sections onto the chuck in a cryostat using water as opposed to OCT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Mail Bring photos to life! New PhotoMail makes sharing a breeze. --------------------------------- Relax. Yahoo! Mail virus scanning helps detect nasty viruses! From Malcolm.McCallum <@t> tamut.edu Fri Mar 10 08:05:51 2006 From: Malcolm.McCallum <@t> tamut.edu (Malcolm McCallum) Date: Fri Mar 10 08:09:35 2006 Subject: [Histonet] cryostat mounting Message-ID: I missed the original question, but if using cryostat and trying to mount tissue slide, why not just have the prepped slide at room temperature and then place the frozen section on it. The second the section hits the slide it will melt and capillary action will hold it to the slide. THats the way we did years ago (haven't used cryostat in a long time). ONly problem may come from melting and freezing the tissue if you need to refreeze the section. Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-233-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Fri 3/10/2006 7:50 AM To: histonet@lists.utsouthwestern.edu Subject: Fwd: Re: [Histonet] cryostat mounting [As shared response] Rene J Buesa wrote: Date: Fri, 10 Mar 2006 05:47:20 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] cryostat mounting To: 0104684s Hi Joseph: Distilled water is what I always used when doing direct immunofluorescence (DIF) in skin biopsies, either punch or shave. I did it in the following way: 1- you set the holder in the thermal absorption bar in the side of the cryostat 2- you should have at least 1 pair of fine tipped forceps also inside the cryostat so they are cold enough 3- you hold a dropper with distilled water with your left hand and with your right hand you hold the skin biopsy with the fine tipped forceps 4- you add a small amount of water (about 0.5 mL) on the chuck and as the water begins to solidify you set the skin sample over that drop of water that is starting to become ice 5- once the skin is in place on the water/ice interface, you keep adding water and building a small and round ice block over it 6- the ones I enjoyed most doing were those around the skin shave biopsies.It was like building a cocoon around them! 7- once the skin biopsy is totally covered with the ice, you take it in the chuck to the cryostat and start sectioning. The sections (over a very cold permanent or steel blade) slide beautifully and when picked up with the slide (always much wamer) immediately melts out leaving the skin section on it. I personally prefer cutting skin biopsis for DIF when they are embedded in water, rather than in OCT ("too messy" for my preference). Hope this description will help you! Ren? J. 0104684s wrote: Has anyone any experience of mounting frozen tissue sections onto the chuck in a cryostat using water as opposed to OCT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Mail Bring photos to life! New PhotoMail makes sharing a breeze. --------------------------------- Relax. Yahoo! Mail virus scanning helps detect nasty viruses! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From igor.nasonkin <@t> jhmi.edu Fri Mar 10 08:52:33 2006 From: igor.nasonkin <@t> jhmi.edu (IGOR NASONKIN) Date: Fri Mar 10 08:53:36 2006 Subject: [Histonet] how to unfold section after mounting? Message-ID: <66776412de32.44114c61@jhmimail.jhmi.edu> dear histonetters, Does anyone have experience trying to unfold frozen (30 micron) section after IF staining and mounting (dried overnight, mounted in dpx after dehydration)? My guess is once it binds it is bound forever. A small piece with important and nice staining folded on itself, it could be seen only after high mag. Wondering if the section could be somehow separated from fisher brand superfrost plus slide by light steaming or with excess of Triton or else to remount. would appreciate ideas or practical advice, thanks! Dr. Igor O. Nasonkin Ph.D. Postdoctoral Fellow Pathology/Neuropathology Johns Hopkins University Ross Research Bldg Rm 563 720 Rutland Ave Baltimore MD 21205 tel: 617-388-4104 Igor.Nasonkin@jhmi.edu From kemlo.rogerson <@t> waht.swest.nhs.uk Fri Mar 10 09:03:30 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Mar 10 09:03:49 2006 Subject: [Histonet] how to unfold section after mounting? Message-ID: Dunno if this helps, probably not, but if I had sections that had curled I put them onto the glass slide, flooded it with alcohol and then put the sections back onto the water bath; the differences in something made the sections uncurl. I can't remember what the differences were; density? Viscosity? Anyway what ever it was the section uncurled; hope that helps. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: IGOR NASONKIN [mailto:igor.nasonkin@jhmi.edu] Sent: Friday, March 10, 2006 2:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] how to unfold section after mounting? dear histonetters, Does anyone have experience trying to unfold frozen (30 micron) section after IF staining and mounting (dried overnight, mounted in dpx after dehydration)? My guess is once it binds it is bound forever. A small piece with important and nice staining folded on itself, it could be seen only after high mag. Wondering if the section could be somehow separated from fisher brand superfrost plus slide by light steaming or with excess of Triton or else to remount. would appreciate ideas or practical advice, thanks! Dr. Igor O. Nasonkin Ph.D. Postdoctoral Fellow Pathology/Neuropathology Johns Hopkins University Ross Research Bldg Rm 563 720 Rutland Ave Baltimore MD 21205 tel: 617-388-4104 Igor.Nasonkin@jhmi.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From funderwood <@t> mcohio.org Fri Mar 10 09:08:58 2006 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Fri Mar 10 09:09:22 2006 Subject: [Histonet] how to unfold section after mounting? Message-ID: A couple weeks ago I received a flyer, I believe it was from Newcomer supply,concerning a new product they have . It's supposed to be able to remove a mounted section from the slide. Phooey, I knew I should have saved the literature. I'm sure if you call them and describe what you're looking for they'll be able to help. Newcomer Supply 1.800.383.7799 Fred >>> IGOR NASONKIN 03/10/06 09:52AM >>> dear histonetters, Does anyone have experience trying to unfold frozen (30 micron) section after IF staining and mounting (dried overnight, mounted in dpx after dehydration)? My guess is once it binds it is bound forever. A small piece with important and nice staining folded on itself, it could be seen only after high mag. Wondering if the section could be somehow separated from fisher brand superfrost plus slide by light steaming or with excess of Triton or else to remount. would appreciate ideas or practical advice, thanks! Dr. Igor O. Nasonkin Ph.D. Postdoctoral Fellow Pathology/Neuropathology Johns Hopkins University Ross Research Bldg Rm 563 720 Rutland Ave Baltimore MD 21205 tel: 617-388-4104 Igor.Nasonkin@jhmi.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Fri Mar 10 09:20:01 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Mar 10 09:20:01 2006 Subject: [Histonet] how to unfold section after mounting? Message-ID: Isn't it the eddies formed from the alcohol/water mixing - rather like Marilyn Monroe's skirt blowing up. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:kemlo.rogerson@waht.swest.nhs.uk] Sent: 10 March 2006 15:04 To: 'Igor.Nasonkin@jhmi.edu'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] how to unfold section after mounting? Dunno if this helps, probably not, but if I had sections that had curled I put them onto the glass slide, flooded it with alcohol and then put the sections back onto the water bath; the differences in something made the sections uncurl. I can't remember what the differences were; density? Viscosity? Anyway what ever it was the section uncurled; hope that helps. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: IGOR NASONKIN [mailto:igor.nasonkin@jhmi.edu] Sent: Friday, March 10, 2006 2:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] how to unfold section after mounting? dear histonetters, Does anyone have experience trying to unfold frozen (30 micron) section after IF staining and mounting (dried overnight, mounted in dpx after dehydration)? My guess is once it binds it is bound forever. A small piece with important and nice staining folded on itself, it could be seen only after high mag. Wondering if the section could be somehow separated from fisher brand superfrost plus slide by light steaming or with excess of Triton or else to remount. would appreciate ideas or practical advice, thanks! Dr. Igor O. Nasonkin Ph.D. Postdoctoral Fellow Pathology/Neuropathology Johns Hopkins University Ross Research Bldg Rm 563 720 Rutland Ave Baltimore MD 21205 tel: 617-388-4104 Igor.Nasonkin@jhmi.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Fri Mar 10 09:20:23 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Fri Mar 10 09:21:43 2006 Subject: [Histonet] how to unfold section after mounting? Message-ID: It's called Mount-Quick but I think it is more a tissue transfer medium, but I could be wrong. Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fred Underwood Sent: Friday, March 10, 2006 10:09 AM To: Igor.Nasonkin@jhmi.edu; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] how to unfold section after mounting? A couple weeks ago I received a flyer, I believe it was from Newcomer supply,concerning a new product they have . It's supposed to be able to remove a mounted section from the slide. Phooey, I knew I should have saved the literature. I'm sure if you call them and describe what you're looking for they'll be able to help. Newcomer Supply 1.800.383.7799 Fred >>> IGOR NASONKIN 03/10/06 09:52AM >>> dear histonetters, Does anyone have experience trying to unfold frozen (30 micron) section after IF staining and mounting (dried overnight, mounted in dpx after dehydration)? My guess is once it binds it is bound forever. A small piece with important and nice staining folded on itself, it could be seen only after high mag. Wondering if the section could be somehow separated from fisher brand superfrost plus slide by light steaming or with excess of Triton or else to remount. would appreciate ideas or practical advice, thanks! Dr. Igor O. Nasonkin Ph.D. Postdoctoral Fellow Pathology/Neuropathology Johns Hopkins University Ross Research Bldg Rm 563 720 Rutland Ave Baltimore MD 21205 tel: 617-388-4104 Igor.Nasonkin@jhmi.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo.rogerson <@t> waht.swest.nhs.uk Fri Mar 10 09:27:17 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Mar 10 09:27:32 2006 Subject: [Histonet] how to unfold section after mounting? Message-ID: If it wasn't a flippant remark thereby exposing me to being told off by people, I'd reply to that but I can't so I won't, but it would have been amusing. -----Original Message----- From: Marshall Terry Dr, Consultant Histopathologist [mailto:Terry.Marshall@rothgen.nhs.uk] Sent: Friday, March 10, 2006 3:20 PM To: Kemlo Rogerson; Igor.Nasonkin@jhmi.edu; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] how to unfold section after mounting? Isn't it the eddies formed from the alcohol/water mixing - rather like Marilyn Monroe's skirt blowing up. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:kemlo.rogerson@waht.swest.nhs.uk] Sent: 10 March 2006 15:04 To: 'Igor.Nasonkin@jhmi.edu'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] how to unfold section after mounting? Dunno if this helps, probably not, but if I had sections that had curled I put them onto the glass slide, flooded it with alcohol and then put the sections back onto the water bath; the differences in something made the sections uncurl. I can't remember what the differences were; density? Viscosity? Anyway what ever it was the section uncurled; hope that helps. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: IGOR NASONKIN [mailto:igor.nasonkin@jhmi.edu] Sent: Friday, March 10, 2006 2:53 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] how to unfold section after mounting? dear histonetters, Does anyone have experience trying to unfold frozen (30 micron) section after IF staining and mounting (dried overnight, mounted in dpx after dehydration)? My guess is once it binds it is bound forever. A small piece with important and nice staining folded on itself, it could be seen only after high mag. Wondering if the section could be somehow separated from fisher brand superfrost plus slide by light steaming or with excess of Triton or else to remount. would appreciate ideas or practical advice, thanks! Dr. Igor O. Nasonkin Ph.D. Postdoctoral Fellow Pathology/Neuropathology Johns Hopkins University Ross Research Bldg Rm 563 720 Rutland Ave Baltimore MD 21205 tel: 617-388-4104 Igor.Nasonkin@jhmi.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From KDwyer3322 <@t> aol.com Fri Mar 10 09:32:09 2006 From: KDwyer3322 <@t> aol.com (KDwyer3322@aol.com) Date: Fri Mar 10 09:32:17 2006 Subject: [Histonet] Texas Society for Histotechnology Symposum/Convention Message-ID: <90.70948223.3142f5f9@aol.com> To All, The Texas Society for Histotechnology will be holding is annual convention March 31-April 2, 2006 in Corpus Christi Texas. For hotel information and program please go to txsh.org. Currently the hotel block is full but other hotel rooms are available around the city. Please contact Judy Webb at 817-927-1024 or Veronica Davis at 972-579-8291 for more information. Thanks and we hope to see you there. From d.fuster <@t> ub.edu Fri Mar 10 09:54:09 2006 From: d.fuster <@t> ub.edu (Dolors Fuster) Date: Fri Mar 10 09:54:26 2006 Subject: [Histonet] Normal solution Message-ID: <4411A121.9020104@ub.edu> *Hi histonetters! I was looking in archives how to made a Normal solution and found the following explanation:* ok....here we go. a 1N solution equals 1 gram-equivalent weight of compound in a liter of water. the gram-equivalent weight is determined by dividing the molecular weight by the number of hydrogen ions (or -OH groups) per formula. so, in the case of sodium hydroxide (NaOH....a single hydroxyl group) the gram-equivalent weight is 40g (ok....i'm rounding a bit for simplicity). so a 1N solution would be 40g/L.....therefore, a 2N solution would be 80g/L and a 5N solution would be 200g/L. carrie kyle-byrne ucdavis-cahfs *It's a really good explanation for me but I still have a question. Have I to follow the same procedure with liquids? What have I to do if I need a Normal solution of hydrocloric acid, nitric acid, iso-propylic alcohol or any other liquid compound? Have I to use the same amount of ml than gr used with solids applying the same formula? Thanks in advance * * Dolors Fuster T?cnic Especialista en Anatomia Patologica i Citologia Facultat de Medicina Dep. d'Anatomia i Embriologia Humana Universitat de Barcelona* From bruyntjes <@t> voeding.tno.nl Fri Mar 10 10:19:38 2006 From: bruyntjes <@t> voeding.tno.nl (Bruijntjes, J.P.) Date: Fri Mar 10 10:19:51 2006 Subject: [Histonet] vibratome sectioning of brain tissues Message-ID: <3B070848E7C2204F9DEB8BCFD767728004A698C3@ntexch1.voeding.tno.nl> Hi all A colleague of me works with rat brains and tries to prepare vibratome section but with hardly no result. Main problem is thin/thick sections. This is what she has done with the tissues ect: Brain tissue: perfused with and fixed pre-embedding in 4% Formaline Embedding procedure: -during 24 H hemispheres were washed in running tap water -during the following 24 H hemispheres were infiltrated with gelatin (from bovine skin type B) solution, 12.5% w/w at 37? -during the next 24 H hemispsheres were infiltrated with gelatin, 25% w/w at 37? -after coagulation (approx 30 min) at 2-10 ? gelatin blocks were further fixed during 4 days in 4% formaline (2-10 ?). A (new) vibratome, HM 650 V, was used for sectioning. Intended section thickness: 35 ?m The buffertray was filled with PBS and crushed ice She have tried many different combinations of the following settings: -knife angle: 10-40 ? (approx) -frequency: 30-100 Hz -amplitude: 0.3-1.2 mm -speed: 1.5-5.0 mm/s She is running out of ideas. Suggestions are very welcome!!! Joost Bruijntjes TNO Quality of Life Toxicology and Applied Pharmacology Zeist Holland This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html From rjbuesa <@t> yahoo.com Fri Mar 10 10:36:38 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 10 10:36:42 2006 Subject: [Histonet] Normal solution In-Reply-To: <4411A121.9020104@ub.edu> Message-ID: <20060310163638.78622.qmail@web61214.mail.yahoo.com> No, you cannot. The thing is that in order to have the needed MASS from a liquid chemical you have to introduce in the calculation 2 other variables: 1- density of the chemical (= g/mL), and 2- concentration (% of the chemical in liquid form). For example: lets suppose you want to prepare a 1 Normal solution of HCl (hydrochloric acid). In this case: the molecular weight (MW) = 36.5 the density = 1.19 g/mL (find that in the label) and purity (or concentration) = 37% (also found in the label). So in order to have a 1N solution of HCl you will need: 36.5 g DIVIDED by (1.19 g/mL x 0.37) = 82.9 mL of H Cl + distilled water UP TO 1,000 mL Hope I have been apble to explain it! Ren? J. Dolors Fuster wrote: *Hi histonetters! I was looking in archives how to made a Normal solution and found the following explanation:* ok....here we go. a 1N solution equals 1 gram-equivalent weight of compound in a liter of water. the gram-equivalent weight is determined by dividing the molecular weight by the number of hydrogen ions (or -OH groups) per formula. so, in the case of sodium hydroxide (NaOH....a single hydroxyl group) the gram-equivalent weight is 40g (ok....i'm rounding a bit for simplicity). so a 1N solution would be 40g/L.....therefore, a 2N solution would be 80g/L and a 5N solution would be 200g/L. carrie kyle-byrne ucdavis-cahfs *It's a really good explanation for me but I still have a question. Have I to follow the same procedure with liquids? What have I to do if I need a Normal solution of hydrocloric acid, nitric acid, iso-propylic alcohol or any other liquid compound? Have I to use the same amount of ml than gr used with solids applying the same formula? Thanks in advance * * Dolors Fuster T?cnic Especialista en Anatomia Patologica i Citologia Facultat de Medicina Dep. d'Anatomia i Embriologia Humana Universitat de Barcelona* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Mail Bring photos to life! New PhotoMail makes sharing a breeze. From ree3 <@t> leicester.ac.uk Fri Mar 10 10:57:45 2006 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri Mar 10 10:57:57 2006 Subject: [Histonet] tunel kits Message-ID: I am after a Tunel kit that works on formalin fixed paraffin processed sections of mouse tissues, many thanks. Richard Edwards MRC TOXICOLOGY UNIT LEICESTER. U.K..... From fawn <@t> cs.cmu.edu Fri Mar 10 11:35:15 2006 From: fawn <@t> cs.cmu.edu (Fawn Jones) Date: Fri Mar 10 11:35:27 2006 Subject: [Histonet] Cutting foams with microtome Message-ID: <4411B8D3.4060304@cs.cmu.edu> Dear histonetters, I have someone using my lab who is cutting some paraffin embedded foam infiltrated with cells. She is having difficulty getting any sections as the foam is falling apart. Does anybody have a technique for this type of material that they would like to share? Fawn Jones From nair.ashwin <@t> gmail.com Fri Mar 10 11:52:47 2006 From: nair.ashwin <@t> gmail.com (Ashwin Nair) Date: Fri Mar 10 11:53:04 2006 Subject: [Histonet] Cutting foams with microtome In-Reply-To: <4411B8D3.4060304@cs.cmu.edu> References: <4411B8D3.4060304@cs.cmu.edu> Message-ID: Hi, I tried sectioning PLLA scaffolds using frozen embedding technique and it worked ok. Its painfully difficult. I believe tape transfer is a better way, I am not sure as I have not done it. Linda, at Clemson Univ suggested this a while back when I had some trouble with sectioning. Ashwin On 3/10/06, Fawn Jones wrote: > > Dear histonetters, > > I have someone using my lab who is cutting some paraffin embedded foam > infiltrated with cells. She is having difficulty getting any sections > as the foam is falling apart. Does anybody have a technique for this > type of material that they would like to share? > > Fawn Jones > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Thank you. Ashwin Nair From lcjunca <@t> gmail.com Fri Mar 10 11:55:10 2006 From: lcjunca <@t> gmail.com (Luiz Carlos Junqueira) Date: Fri Mar 10 11:55:27 2006 Subject: [Histonet] =?iso-8859-1?q?FURTHER_INFORMATION_REGARDING_THE_PICR?= =?iso-8859-1?q?OS=CDRIUS-POLARIZATION_METHOD?= Message-ID: <001401c6446b$cb81f900$191596c8@nomenxse3cen4t> FURTHER INFORMATION REGARDING THE PICROS?RIUS-POLARIZATION METHOD As I have found in Internet questions regarding the use of the picros?rius-polarization method, these additional informations regarding its use may be useful. 1-This method considered to be "one of the best understood techniques of collagen histochemistry" presentes the following characteristics: 2-The picros?rius solution can last for more than 10 years in a dark bottle. Slides stained by it do not fade in 20 years; 3-I always pre-stain slides of paraffin embedded material initially with PS and after brief washing with Harris Hematoxylin. I feel that this produces far better results; 4-If you use cheap photographic polaroid filters you will obtain a nice blue background, but if you use them repeatedly, for long periods, you might end up with an inflammation of the conjunctiva. With more expensive microscopic polarizing filters (I use a Nikon) you will get a totally black background and with a much higher resolution; 5-This will permit you to observe birefringence in basement membranes. See Junqueira et.al-Evidence for collagen molecular orientation in basement membranes-Histochem. J.15: 785,1983; 6-I tried several ways of staining resin (historesin) embedded material with no success, probably due to S?rius high molecular weight (+-1000); 7-Picros?rius stains cartilages collagen well only after pre-digestion with papain, that extracts a cloud of proteoglycans. The figures thus obtained, remember the Saint Chapel glass windows in a sunny day; 8-Thin birrefringent collagen fibers can be adult reticular fibers (type III) or young collagen (type I) fibers. Thus, distinction between these two types of collagen fibers cannot be made during developing processes; 9-Not all S?rius Red batches work well. The only I use is the F3B200 from Mowbay Chemical Co, Union New Jersey USA. Before its use in Histology, its cost was approximately U$30,00 per pound. 10-Compared with Picros?rius, the trichrome staining procedures tested, do not stain collagen specifically or electively; 11-Picros?rius stain collagen specifically. The only exceptions are some rare secretory salivary gland cells that contain basic cytoplasmic proteins, however they are not birrefringent. In some species the queratin present in the queratinized layer of the skin can light up. No other exceptions were observed from fishes to mammals. To distinguish birrefringent from refringent material it is necessary to rotate the specimen using a microscope equipped with circularly motion. Only birrefringent material changes its color; 12-Both formaldeyde and Bouin give good results with picros?rius. Very thin sections do not give good information regarding the dispersed disposition of collagen in these sections; 13-The Picros?rius-polarization method has been widely used in research to study collagen as shown by the 57.000 references in "S?rius collagen" in Google in 06/3/2006. Figures obtained with this procedure are of exceptionally high didactic quality, and would, with great advantage, substitute the usual haematoxylin-eosin or trichrome preparations. Despite this, figures using this method described approximately 35 years ago, appeared only in 35 micrographs in a recent text book (Basic Histology, Junqueira & Carneiro, 11th edition, McGraw-Hill). More information on this method is obtainable in the review that presents most of the literature regarding its physico-chemical basis and its use in other fields (Montes and Junqueira: The use of picros?rius polarization method for the study of biopatology of collagen. Mem. Inst. Oswaldo Cruz Vol. 86, Suppl.III, 1-11,1991). For futher information my address is: L.C.Junqueira Po?os de Caldas-MG- Brazil From bob-meyer <@t> northwestern.edu Fri Mar 10 12:01:13 2006 From: bob-meyer <@t> northwestern.edu (bob-meyer@northwestern.edu) Date: Fri Mar 10 12:01:20 2006 Subject: [Histonet] tunel kits Message-ID: <20060310180113.64AC335C90@casbah.it.northwestern.edu> We use the ApopTag Plus Peroxidase In Situ apoptosis detection kit from Chemicon International. Cat # S7101. Bob Meyer Northwestern University ==============Original message text=============== On Fri, 10 Mar 2006 4:57:45 pm +0000 "Edwards, R.E." wrote: I am after a Tunel kit that works on formalin fixed paraffin processed sections of mouse tissues, many thanks. Richard Edwards MRC TOXICOLOGY UNIT LEICESTER. U.K..... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ===========End of original message text=========== From cpgarcia <@t> salk.edu Fri Mar 10 12:07:33 2006 From: cpgarcia <@t> salk.edu (Carlos G. Perez-Garcia) Date: Fri Mar 10 12:07:15 2006 Subject: [Histonet] ish Message-ID: <16C98B4F-7275-4B91-9879-8A0919A737E5@salk.edu> hi all i am looking for a good protocol for in situ hybridization in Bouin fixed tissue. If anybody has one i would really appreciate it. best carlos Carlos G. Perez-Garcia, Ph.D. Molecular Neurobiology Lab (MNL-O) The Salk Institute 10010 North Torrey Pines Road 92037 La Jolla, CA USA cpgarcia@salk.edu Fax: 858 558 6207 From dobbin <@t> upei.ca Fri Mar 10 11:16:03 2006 From: dobbin <@t> upei.ca (Greg Dobbin) Date: Fri Mar 10 12:11:13 2006 Subject: [Histonet] Normal solution In-Reply-To: <20060310163638.78622.qmail@web61214.mail.yahoo.com> References: <4411A121.9020104@ub.edu> Message-ID: <44117C13.2482.1012648@acad1.cs.upei.ca> Good explanation Rene! But I would just caution Dolors to ALWAYS add acid to water never water to acid (too much heat generated-could erupt all over you). Add the 82.9 ml of conc. HCl to say 800 ml of water, then quench to 1 L. Cheers! Greg Date sent: Fri, 10 Mar 2006 08:36:38 -0800 (PST) From: Rene J Buesa To: d.fuster@ub.edu, histonet@lists.utsouthwestern.edu Copies to: Subject: Re: [Histonet] Normal solution > No, you cannot. The thing is that in order to have the needed MASS > from a liquid chemical you have to introduce in the calculation 2 > other variables: > 1- density of the chemical (= g/mL), and > 2- concentration (% of the chemical in liquid form). > For example: lets suppose you want to prepare a 1 Normal solution of > HCl (hydrochloric acid). In this case: the molecular weight (MW) = > 36.5 the density = 1.19 g/mL (find that in the label) and purity (or > concentration) = 37% (also found in the label). So in order to have > a 1N solution of HCl you will need: > > 36.5 g DIVIDED by (1.19 g/mL x 0.37) = 82.9 mL of H Cl + distilled > water UP TO 1,000 mL Hope I have been apble to explain it! Ren? J. > > Dolors Fuster wrote: > *Hi histonetters! > > I was looking in archives how to made a Normal solution and found the > following explanation:* > > ok....here we go. a 1N solution equals 1 gram-equivalent weight of > compound in a liter of water. the gram-equivalent weight is determined > by dividing the molecular weight by the number of hydrogen ions (or > -OH groups) per formula. so, in the case of sodium hydroxide > (NaOH....a single hydroxyl group) the gram-equivalent weight is 40g > (ok....i'm rounding a bit for simplicity). so a 1N solution would be > 40g/L.....therefore, a 2N solution would be 80g/L and a 5N solution > would be 200g/L. > > carrie kyle-byrne > ucdavis-cahfs > > > *It's a really good explanation for me but I still have a question. > Have I to follow the same procedure with liquids? What have I to do if > I need a Normal solution of hydrocloric acid, nitric acid, > iso-propylic alcohol or any other liquid compound? Have I to use the > same amount of ml than gr used with solids applying the same formula? > > Thanks in advance > > * > * > Dolors Fuster > T?cnic Especialista en Anatomia Patologica i Citologia > Facultat de Medicina > Dep. d'Anatomia i Embriologia Humana > Universitat de Barcelona* > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Yahoo! Mail > Bring photos to life! New PhotoMail makes sharing a breeze. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Fri Mar 10 13:22:18 2006 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Mar 10 13:22:22 2006 Subject: [Histonet] Friday Hour of Fuming Message-ID: I suggest we have a Friday Hour of Fuming in order to allow us to rid ourselves of the mental flotsam and jetsam that have accumulated during the week. For example.... I'd like to Fume against "lip drip". It would seem to me that after years and years of R&D, someone could perfect a liter/gallon container that didn't piddle all over everything when pouring. The beaker people fixed that YEARS ago. A small adjustment in the shape of the lip would solve that annoyance! I feel SO much better! Next? Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services POBox 4700 Albuquerque, NM 87196 (505)841-2576 (505)841-2518 FAX From funderwood <@t> mcohio.org Fri Mar 10 13:29:07 2006 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Fri Mar 10 13:29:39 2006 Subject: [Histonet] Friday Hour of Fuming Message-ID: Along those lines. Have you ever ordered a pitcher of your favorite beverage and had your waitress (excuse me, wait person) pour the drink out of the side of the pitcher. Instead of the front like it was designed. >>> "Breeden, Sara" 03/10/06 02:22PM >>> I suggest we have a Friday Hour of Fuming in order to allow us to rid ourselves of the mental flotsam and jetsam that have accumulated during the week. For example.... I'd like to Fume against "lip drip". It would seem to me that after years and years of R&D, someone could perfect a liter/gallon container that didn't piddle all over everything when pouring. The beaker people fixed that YEARS ago. A small adjustment in the shape of the lip would solve that annoyance! I feel SO much better! Next? Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services POBox 4700 Albuquerque, NM 87196 (505)841-2576 (505)841-2518 FAX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Fri Mar 10 13:31:28 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Mar 10 13:31:36 2006 Subject: [Histonet] vibratome sectioning of brain tissues Message-ID: <09C945920A6B654199F7A58A1D7D1FDE01717687@lsexch.lsmaster.lifespan.org> I often section rat brains on the vibratome, and I must say my preparation is far simpler than yours. After fixation in formalin I rinse briefly in PBS, transfer the brain to 3% agarose at 37 degrees for 15 minutes, then place in 6% agarose in a plastic embedding mold and transfer to the refrigerator (4 degrees) until fully gelled (about 10 minutes). I remove the block from the mold, trim its base by hand with a disposable microtome blade to ensure a flat surface, blot that surface dry, and cement it to a metal block with cyanoacrylate glue using gentle pressure for about a minute, then place it in the block holder and begin sectioning. The whole process from fixed specimen to start of sectioning takes about 40 minutes. Vibratome specimens don't have to be infiltrated. They only have to be physically supported by the surrounding medium. I use the 3% agarose treatment in order to create a better bond between the embedding medium and the surface of the tissue. I don't like gelatin. It doesn't yield as rigid a block as agarose, even at higher concentrations. I use a 20 degree knife angle; a fairly high amplitude (7-8 out of a possible 10); and a slow speed (2-3 out of a possible 10). The water bath contains PBS with ice. Thick/thin sectioning has three likely causes. First, the block is insufficiently rigid, that is, of too low a density; second, the agarose block is not firmly bonded to the block holder surface; or third, the blade extends too high above the blade holder, so that the blade flexes as it cuts through the block. This causes the blade to dig into the block, forming a thicker section, which results in the next section being too thin, because some of the tissue that belonged to the second section went into the first section. Regards, Paul Monfils > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > Bruijntjes, J.P. > Sent: Friday, March 10, 2006 8:19 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] vibratome sectioning of brain tissues > > Hi all > > > > A colleague of me works with rat brains and tries to prepare vibratome > section but with hardly no result. > > Main problem is thin/thick sections. > > > > This is what she has done with the tissues ect: > > > > Brain tissue: perfused with and fixed pre-embedding in 4% Formaline > > > > Embedding procedure: > > -during 24 H hemispheres were washed in running tap water > > -during the following 24 H hemispheres were infiltrated with gelatin (from > bovine skin > > type B) solution, 12.5% w/w at 37? > > -during the next 24 H hemispsheres were infiltrated with gelatin, 25% w/w > at 37? > > -after coagulation (approx 30 min) at 2-10 ? gelatin blocks were further > fixed during 4 > > days in 4% formaline (2-10 ?). > > > > A (new) vibratome, HM 650 V, was used for sectioning. > > Intended section thickness: 35 ?m > > The buffertray was filled with PBS and crushed ice > > > > She have tried many different combinations of the following settings: > > -knife angle: 10-40 ? (approx) > > -frequency: 30-100 Hz > > -amplitude: 0.3-1.2 mm > > -speed: 1.5-5.0 mm/s > > > > She is running out of ideas. Suggestions are very welcome!!! > > > > > > Joost Bruijntjes > > TNO Quality of Life > > Toxicology and Applied Pharmacology > > Zeist > > Holland > > > > > > > This e-mail and its contents are subject to the DISCLAIMER at > http://www.tno.nl/disclaimer/email.html > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Eric.C.Kellar <@t> questdiagnostics.com Fri Mar 10 13:32:23 2006 From: Eric.C.Kellar <@t> questdiagnostics.com (Kellar, Eric C) Date: Fri Mar 10 13:32:34 2006 Subject: [Histonet] RE: cutting foams with microtome Message-ID: <6843061CE6B98E4B96590D4F299618F801583BB5@qdcws0117.us.qdx.com> Fawn, If, you are speaking of the open celled, blue foam biopsy pads, then yes. I have had some success cutting them, but it's tricky. I face the foam pad embedded cassette at the microtome. I then place a warm water soaked Kimwipe over the faced surface (while still in the chuck holder) for a few minutes. Remove the Kimwipe and spray the entire surface with Frostbite(1,1,1,2 - tetrafluoroethane). The colder the paraffin becomes, the better the foam pad seems to section. The use of low profile, disposable blades also aid in the sectioning. Hope this helps! Eric C. Kellar Histology/Immunohistochemistry Quest Diagnostics Inc. Miami Dear histonetters, I have someone using my lab who is cutting some paraffin embedded foam infiltrated with cells. She is having difficulty getting any sections as the foam is falling apart. Does anybody have a technique for this type of material that they would like to share? Fawn Jones ============================================================================== The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. ============================================================================== From PMonfils <@t> Lifespan.org Fri Mar 10 13:35:45 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Mar 10 13:35:52 2006 Subject: [Histonet] Cutting foams with microtome Message-ID: <09C945920A6B654199F7A58A1D7D1FDE01717689@lsexch.lsmaster.lifespan.org> I'm not entirely sure what is meant by "foam", but I assume it is one of the polymer materials which are formed into implantable "sponges", implanted in animals, and later harvested? If so, I section such materials frequently. The first consideration of course is that the specific polymer is resistant to the reagents used in processing. Some polymers are highly resistant to typical hydrocarbon clearing agents and some are not. Some will undergo various degrees of breakdown in such solvents, and some may dissolve completely. Some polymers, in order to get them into paraffin without damage, must be processed with an alternative clearing agent. Having cleared that hurdle, the principle challenge is getting the sponge thoroughly infiltrated with paraffin. Some polymer sponges have a more "open" configuration than others, that is to say something entering a given pore of the sponge could find its way through to the other side of the specimen without running into "dead ends". The more open the structure is, the more freely solvents can flow through the material. But some such materials have closed cells or dead end channels which make infiltration more difficult. Also, such closed areas can trap water, or air. After fixation is complete I transfer such materials (in cassettes if they will fit) to 70% ethanol, and place them in the vacuum oven (no heat, just full vacuum) until bubbles stop rising from the specimens (15 or 20 minutes is usually sufficient). I then process them on a Tissue-Tek VIP processor, using alternating vacuum and pressure on all stations, on a 40 hour cycle (quitting time of day 1 until morning of day 3). Then embed as usual, and I find they section with little difficulty. I allow the sections to float on the water bath for 2 to 3 minutes before picking them up, and I use a chrome gelatin product (Sta-On from Surgipath) in the water bath to attach the sections to the slides. Positively charged slides won't work well on this kind of material because polymers don't have the complementary net negative charge that tissues typically have. Paul M. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Fawn > Jones > Sent: Friday, March 10, 2006 9:35 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Cutting foams with microtome > > Dear histonetters, > > I have someone using my lab who is cutting some paraffin embedded foam > infiltrated with cells. She is having difficulty getting any sections > as the foam is falling apart. Does anybody have a technique for this > type of material that they would like to share? > > Fawn Jones > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From jqb7 <@t> cdc.gov Fri Mar 10 13:39:05 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Fri Mar 10 13:43:51 2006 Subject: [Histonet] Friday Hour of Fuming Message-ID: Yep, but it was explained to me that it was to allow for fresh ice to make its way into your glass along with the beverage. Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fred Underwood Sent: Friday, March 10, 2006 2:29 PM To: histonet@lists.utsouthwestern.edu; sbreeden@nmdamail.nmsu.edu Subject: Re: [Histonet] Friday Hour of Fuming Along those lines. Have you ever ordered a pitcher of your favorite beverage and had your waitress (excuse me, wait person) pour the drink out of the side of the pitcher. Instead of the front like it was designed. >>> "Breeden, Sara" 03/10/06 02:22PM >>> I suggest we have a Friday Hour of Fuming in order to allow us to rid ourselves of the mental flotsam and jetsam that have accumulated during the week. For example.... I'd like to Fume against "lip drip". It would seem to me that after years and years of R&D, someone could perfect a liter/gallon container that didn't piddle all over everything when pouring. The beaker people fixed that YEARS ago. A small adjustment in the shape of the lip would solve that annoyance! I feel SO much better! Next? Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services POBox 4700 Albuquerque, NM 87196 (505)841-2576 (505)841-2518 FAX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From glenn_krasinski <@t> yahoo.com Fri Mar 10 14:36:37 2006 From: glenn_krasinski <@t> yahoo.com (Glenn Krasinski) Date: Fri Mar 10 14:36:41 2006 Subject: [Histonet] H2DCFDA and Antifadent.... Message-ID: <20060310203637.62743.qmail@web37511.mail.mud.yahoo.com> I am using H2DCFDA for the detection of cellular reactive oxygen species following treatment with various agents. Cells are subsequently mounted in anti-fadent with DAPI (Slow Fade Gold with DAPI). The green fluorescence appears awfully weak. I have noticed this in past experiments looking at cytochrome c release with FITC detection. Could the antifadent be the culprit? Don't some antifadent products quench fluorescein right-off-the-bat? Would it be best to just do a standard aqueous mount and be extra careful with light exposure? Many thanks for your assistance. Glenn M. Krasinski Senior Associate Scientist I, Biology Attenuon, LLC 11535 Sorrento Valley Road, Suite 401 San Diego, CA 92121 Phone: (858) 720-8797 E-dress: krasinski@attenuon.com --------------------------------- Yahoo! Mail Use Photomail to share photos without annoying attachments. From sjchtascp <@t> yahoo.com Fri Mar 10 15:07:37 2006 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Fri Mar 10 15:07:40 2006 Subject: [Histonet] Cell blocks, Message-ID: <20060310210737.44560.qmail@web38211.mail.mud.yahoo.com> I'm looking for a way to make simple cell blocks. I work in research so I need something that would cover routine cells and cell cultures?? Steve --------------------------------- Yahoo! Mail Bring photos to life! New PhotoMail makes sharing a breeze. From kelseymichele <@t> hotmail.com Fri Mar 10 15:09:22 2006 From: kelseymichele <@t> hotmail.com (Kelsey Peek) Date: Fri Mar 10 15:09:30 2006 Subject: [Histonet] Feelings Message-ID: How does it make you feel when you find pathology in a tissue sample? Does it make you sad or are you just doing your job? _________________________________________________________________ Is your PC infected? Get a FREE online computer virus scan from McAfee® Security. http://clinic.mcafee.com/clinic/ibuy/campaign.asp?cid=3963 From c.ingles <@t> hosp.wisc.edu Fri Mar 10 15:11:53 2006 From: c.ingles <@t> hosp.wisc.edu (Ingles Claire) Date: Fri Mar 10 15:11:59 2006 Subject: [Histonet] Friday Hour of Fuming References: Message-ID: <583D3E9A1E843445BD54E461D1A2F6F317A04F1A@uwhis-xchng2.hosp.wisc.edu> Normally I would join in the Friday Fuming (and probably will on a regular basis in the future), but I want to add a little hope to my fellow hard working histotech. I got a surprise raise today!!! I guess nagging does help sometimes. Our union (yes we HAVE to belong to a union here) has re-negotiated our contract and we were bumped up a pay level. I guess that softens the ego blow that we belong to the same union as the housekeeping and food service people, and not with the rest of the clinical core lab staff union. (I have NO idea how that came about, don't ask). So all those salary surveys may be worth something after all. Keep up the fight! Miracles do happen. Claire Ingles Mohs Clinic UW Madison Hospital ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bartlett, Jeanine Sent: Fri 3/10/2006 1:39 PM To: Fred Underwood; histonet@lists.utsouthwestern.edu; sbreeden@nmdamail.nmsu.edu Subject: RE: [Histonet] Friday Hour of Fuming Yep, but it was explained to me that it was to allow for fresh ice to make its way into your glass along with the beverage. Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fred Underwood Sent: Friday, March 10, 2006 2:29 PM To: histonet@lists.utsouthwestern.edu; sbreeden@nmdamail.nmsu.edu Subject: Re: [Histonet] Friday Hour of Fuming Along those lines. Have you ever ordered a pitcher of your favorite beverage and had your waitress (excuse me, wait person) pour the drink out of the side of the pitcher. Instead of the front like it was designed. >>> "Breeden, Sara" 03/10/06 02:22PM >>> I suggest we have a Friday Hour of Fuming in order to allow us to rid ourselves of the mental flotsam and jetsam that have accumulated during the week. For example.... I'd like to Fume against "lip drip". It would seem to me that after years and years of R&D, someone could perfect a liter/gallon container that didn't piddle all over everything when pouring. The beaker people fixed that YEARS ago. A small adjustment in the shape of the lip would solve that annoyance! I feel SO much better! Next? Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services POBox 4700 Albuquerque, NM 87196 (505)841-2576 (505)841-2518 FAX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.ingles <@t> hosp.wisc.edu Fri Mar 10 15:19:20 2006 From: c.ingles <@t> hosp.wisc.edu (Ingles Claire) Date: Fri Mar 10 15:19:25 2006 Subject: [Histonet] Feelings References: Message-ID: <583D3E9A1E843445BD54E461D1A2F6F317A04F1B@uwhis-xchng2.hosp.wisc.edu> It's a little of both for me. When we lab rats see an unusual cancer we think it's really cool from an academic point of view. But when we get large layer after large layer from the same patient, we feel sorry for them. But I work in a Mohs lab and all of our specimens are of a stat nature. Most of our patient base is older as well, and when I see a frail old lady wheeled in for her third excision that day, my heart goes out. We also have a lot of chronic patients that keep coming back every few months, and when we see them on the schedule for the day, my co-workers and I just kind of sigh, put on our work faces and are determined to get the patient clear of cancer for the day. To know that we are curing patients on a daily basis helps keep me here though. Claire Ingles UW Moh Clinic Madison WI ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kelsey Peek Sent: Fri 3/10/2006 3:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Feelings How does it make you feel when you find pathology in a tissue sample? Does it make you sad or are you just doing your job? _________________________________________________________________ Is your PC infected? Get a FREE online computer virus scan from McAfee? Security. http://clinic.mcafee.com/clinic/ibuy/campaign.asp?cid=3963 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From failm <@t> musc.edu Fri Mar 10 15:20:28 2006 From: failm <@t> musc.edu (Mildred Fail) Date: Fri Mar 10 15:20:56 2006 Subject: [Histonet] Feelings Message-ID: If I've been given no info just a stain to perform, excitement First, It's look at that!! then it's poor patient But the hem-path doc will tell me a bit about the pt then the reaction is oh poor thing then cool staining. Rena Fail >>> "Kelsey Peek" 03/10/06 04:09PM >>> How does it make you feel when you find pathology in a tissue sample? Does it make you sad or are you just doing your job? _________________________________________________________________ Is your PC infected? Get a FREE online computer virus scan from McAfee? Security. http://clinic.mcafee.com/clinic/ibuy/campaign.asp?cid=3963 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HParker <@t> Skaggs.Net Fri Mar 10 15:26:29 2006 From: HParker <@t> Skaggs.Net (Parker, Helayne) Date: Fri Mar 10 15:27:01 2006 Subject: [Histonet] Pathology Programs Message-ID: <2FABC6145388F24EBA8CBD6EC165BCCB02AFBA1E@mail1-schc.skaggs.net> Hi Gang, I was just wondering if anyone has any input on a good Pathology Program out there. One that is user friendly and good for the clerical people, technical people and MOST IMPORTANTLY the Pathologist. We do about 10K surgical cases a year and 500 cytology cases. Helayne Parker, HT (A.S.C.P.) Histology Section Head Skaggs Community Health Center Branson, Missouri hparker@skaggs.net From LGaliotto <@t> nch.org Fri Mar 10 15:32:06 2006 From: LGaliotto <@t> nch.org (Galiotto, Laura) Date: Fri Mar 10 15:32:11 2006 Subject: [Histonet] Help with Carnoy Message-ID: <270614B321ACB44D8C1D91F4F921FDC3036CBC06@NCH01EX02.nch.org> Is anyone currently using Carnoy for fat dissolution in an attempt to retrieve lymph nodes particularly in colon resections? If so pleases advise of process and disposal. The name of manufacturer or vender would also be helpful. Laura Galiotto, HT (ASCP) Histology Facilitator Northwest Community Hospital 800 West Central Road Arlington Heights, Il 60005 847-618-6190 From Jackie.O'Connor <@t> abbott.com Fri Mar 10 15:58:23 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Mar 10 15:58:47 2006 Subject: [Histonet] Feelings Message-ID: I never liked dealing with live patients - I hated assisting FNA's and bone marrows. In the lab, I only had to put a name to a sample - in the clinic it became a person - not that the samples were treated with any less regard, mind you - - -I treated every specimen like it was gold because it could mean life or death to a person. I just couldn't forget the faces - mice are so much easier - they all have the same face. "Mildred Fail" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/10/2006 03:20 PM To: , cc: (bcc: Jackie M O'Connor/LAKE/GPRD/ABBOTT) Subject: Re: [Histonet] Feelings If I've been given no info just a stain to perform, excitement First, It's look at that!! then it's poor patient But the hem-path doc will tell me a bit about the pt then the reaction is oh poor thing then cool staining. Rena Fail >>> "Kelsey Peek" 03/10/06 04:09PM >>> How does it make you feel when you find pathology in a tissue sample? Does it make you sad or are you just doing your job? _________________________________________________________________ Is your PC infected? Get a FREE online computer virus scan from McAfee? Security. http://clinic.mcafee.com/clinic/ibuy/campaign.asp?cid=3963 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pathrm35 <@t> adelphia.net Fri Mar 10 16:13:53 2006 From: pathrm35 <@t> adelphia.net (pathrm35@adelphia.net) Date: Fri Mar 10 16:13:57 2006 Subject: [Histonet] Tissue Tek questions Message-ID: <3754788.1142028833820.JavaMail.root@web16> Fellow techs, Does anyone have any info or experience with the following Tissue Tek instruments: 1) Tissue Tek Xpress Rapid Tissue Processor 2) Tissue Tek AutoTec Automated Embedding System 3) Tissue Tek Prisma/Film Automated Slide Stainer and Coverslipper Fell free to contact me directly. Thanks, Ron Martin From igor.nasonkin <@t> jhmi.edu Fri Mar 10 16:17:20 2006 From: igor.nasonkin <@t> jhmi.edu (IGOR NASONKIN) Date: Fri Mar 10 16:17:25 2006 Subject: [Histonet] dehydration->xylene->DPX for immunofluorescence? what is your opinion? Message-ID: Dear histonetters, What is your practical experience with using dehydration->xylene->DPX mounting of fluorescently labeled sections? My graduate lab used only aqueous medium for coverslipping fluorescently labeled sections. I always heard alcohol-xylene is a no-no for fluorescence, even here (my postdoc.lab). However my PI insists on using alcohol dehydration and mounting in xylene-based DPX because "xylene removes lipids from brain sections and makes the signal more crisp. I tried both, and both (aqueous and xylene-based methods) work and produce nice fluorescent signal. Is there a general guideline here which method is technically correct? Thans for your response, Igor From jnocito <@t> satx.rr.com Sat Mar 11 06:37:07 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sat Mar 11 06:35:14 2006 Subject: [Histonet] Help with Carnoy References: <270614B321ACB44D8C1D91F4F921FDC3036CBC06@NCH01EX02.nch.org> Message-ID: <002101c64508$82b25ce0$e8bd0b43@yourxhtr8hvc4p> Laura, I started using Carnoy's again because I was being told by my pathologists that I wasn't retrieving all the lymph nodes in colon and breast cases. I hope you have a well-ventilated area to work in because the fumes get pretty strong. We put the used Carnoy's in our alcohol/xylene waste containers to be carried off. Good luck Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Galiotto, Laura" To: Sent: Friday, March 10, 2006 3:32 PM Subject: [Histonet] Help with Carnoy Is anyone currently using Carnoy for fat dissolution in an attempt to retrieve lymph nodes particularly in colon resections? If so pleases advise of process and disposal. The name of manufacturer or vender would also be helpful. Laura Galiotto, HT (ASCP) Histology Facilitator Northwest Community Hospital 800 West Central Road Arlington Heights, Il 60005 847-618-6190 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.375 / Virus Database: 268.2.1/278 - Release Date: 3/9/2006 From jnocito <@t> satx.rr.com Sat Mar 11 06:47:55 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sat Mar 11 06:45:57 2006 Subject: [Histonet] Friday Hour of Fuming References: Message-ID: <003401c6450a$050019c0$e8bd0b43@yourxhtr8hvc4p> My turn. I'm preparing to take the PA exam. The doctors I work for have a private lab and are contracted out to a hospital. They all thought it was a great idea that I start grossing at the hospital every afternoon to increase my knowledge (yeah, ok.) Last Saturday I started loosing my voice and came down with bronchitis. I went to work on Monday and struggled all day while dictating. I called the evening supervisor to tell him I won't be in Tuesday because I was going to the doctor to get checked out. My medical director came in Tuesday and was told by my morning supervisor that I was sick. Thursday, he came in and asked how I was feeling. I told him my voice was getting better. Then he told me that I should have contacted the pathologist scheduled to gross and tell him I was sick. Now I tell you, these two pathologists have their offices side by side. Don't you think my medical director should have told the other pathologist that I was sick? Am I in outer space or something? Reality check here. I told my medical director that at least six people knew (including him) and I ass u me d someone would have passed the word. All I could say was that if I could talk, I would've called. Aagggggggggggg!!!!! ----- Original Message ----- From: "Fred Underwood" To: ; Sent: Friday, March 10, 2006 1:29 PM Subject: Re: [Histonet] Friday Hour of Fuming > Along those lines. Have you ever ordered a pitcher of your favorite > beverage and had your waitress (excuse me, wait person) pour the drink > out of the side of the pitcher. Instead of the front like it was > designed. > >>>> "Breeden, Sara" 03/10/06 02:22PM >>> > I suggest we have a Friday Hour of Fuming in order to allow us to rid > ourselves of the mental flotsam and jetsam that have accumulated > during > the week. For example.... I'd like to Fume against "lip drip". It > would > seem to me that after years and years of R&D, someone could perfect a > liter/gallon container that didn't piddle all over everything when > pouring. The beaker people fixed that YEARS ago. A small adjustment > in > the shape of the lip would solve that annoyance! > > I feel SO much better! Next? > > Sally Breeden, HT(ASCP) > New Mexico Department of Agriculture > Veterinary Diagnostic Services > POBox 4700 > Albuquerque, NM 87196 > (505)841-2576 > (505)841-2518 FAX > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.375 / Virus Database: 268.2.1/278 - Release Date: 3/9/2006 > > From Bartholomew2001 <@t> aol.com Sat Mar 11 09:10:02 2006 From: Bartholomew2001 <@t> aol.com (Bartholomew2001@aol.com) Date: Sat Mar 11 09:10:09 2006 Subject: [Histonet] No Practical for HT in 2007(or after?) Message-ID: <284.6f03cd0.3144424a@aol.com> Good Day Everyone! I was wondering if anyone can confirm that the HT practical will be discontinued in 2007 (and thereafter)? Thanks! From gu.lang <@t> gmx.at Sat Mar 11 09:27:27 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Mar 11 09:27:27 2006 Subject: [Histonet] ISH-question Message-ID: <000301c64520$4e621a80$eeeea8c0@SERVER01> Hi, I fear this is a really silly question, but hope I get an answer: Is Aqua dest. a liquid of high or low stringency? Thank you Gudrun Lang From JWEEMS <@t> sjha.org Sat Mar 11 09:58:15 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Sat Mar 11 09:58:35 2006 Subject: [Histonet] Help with Carnoy References: <270614B321ACB44D8C1D91F4F921FDC3036CBC06@NCH01EX02.nch.org> <002101c64508$82b25ce0$e8bd0b43@yourxhtr8hvc4p> Message-ID: <83AACDB0810528418AA106F9AE9B7F7E01825335@sjhaexc02.sjha.org> Where do you get your chloroform? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Joe Nocito Sent: Sat 3/11/2006 7:37 AM To: Galiotto, Laura; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Help with Carnoy Laura, I started using Carnoy's again because I was being told by my pathologists that I wasn't retrieving all the lymph nodes in colon and breast cases. I hope you have a well-ventilated area to work in because the fumes get pretty strong. We put the used Carnoy's in our alcohol/xylene waste containers to be carried off. Good luck Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Galiotto, Laura" To: Sent: Friday, March 10, 2006 3:32 PM Subject: [Histonet] Help with Carnoy Is anyone currently using Carnoy for fat dissolution in an attempt to retrieve lymph nodes particularly in colon resections? If so pleases advise of process and disposal. The name of manufacturer or vender would also be helpful. Laura Galiotto, HT (ASCP) Histology Facilitator Northwest Community Hospital 800 West Central Road Arlington Heights, Il 60005 847-618-6190 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.375 / Virus Database: 268.2.1/278 - Release Date: 3/9/2006 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From JWEEMS <@t> sjha.org Sat Mar 11 10:01:52 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Sat Mar 11 10:05:10 2006 Subject: [Histonet] Pathology Programs References: <2FABC6145388F24EBA8CBD6EC165BCCB02AFBA1E@mail1-schc.skaggs.net> Message-ID: <83AACDB0810528418AA106F9AE9B7F7E01825336@sjhaexc02.sjha.org> I'm thinking you are asking about computer programs? There are several - Copath the best that I have heard of - designed by a pathologist so it is good for anatomic path. Although I have not used it, I hear good things about it. I am currently using Triple G, which I cannot highly recommend. Good luck, Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Parker, Helayne Sent: Fri 3/10/2006 4:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pathology Programs Hi Gang, I was just wondering if anyone has any input on a good Pathology Program out there. One that is user friendly and good for the clerical people, technical people and MOST IMPORTANTLY the Pathologist. We do about 10K surgical cases a year and 500 cytology cases. Helayne Parker, HT (A.S.C.P.) Histology Section Head Skaggs Community Health Center Branson, Missouri hparker@skaggs.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From poru715 <@t> comcast.net Sat Mar 11 11:46:08 2006 From: poru715 <@t> comcast.net (poru715@comcast.net) Date: Sat Mar 11 11:46:14 2006 Subject: [Histonet] Pathology Programs Message-ID: <031120061746.16857.44130CE00004E43A000041D92207021053CACEC99A9D019F@comcast.net> Copath by far........we have 35K surgicals per year with over 20K cyto. It is also easy to apply other computer programs such as with slide etchers because of easy compatibility. I've used other systems such as Sunquest and Meditech but Copath seems to be the best for the overall Anatomic Path department (ex. cyto, histo, pathologists, and clerical). -------------- Original message -------------- From: "Weems, Joyce" > I'm thinking you are asking about computer programs? > There are several - Copath the best that I have heard of - designed by a > pathologist so it is good for anatomic path. Although I have not used it, I hear > good things about it. > > I am currently using Triple G, which I cannot highly recommend. > > Good luck, > Joyce > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Parker, Helayne > Sent: Fri 3/10/2006 4:26 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Pathology Programs > > Hi Gang, > I was just wondering if anyone has any input on a good Pathology > Program out there. One that is user friendly and good for the clerical > people, technical people and MOST IMPORTANTLY the Pathologist. We do > about 10K surgical cases a year and 500 cytology cases. > > Helayne Parker, HT (A.S.C.P.) > Histology Section Head > Skaggs Community Health Center > Branson, Missouri > hparker@skaggs.net > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > Confidentiality Notice ** The information contained in this message may be > privileged and is confidential information intended for the use of the addressee > listed above. If you are neither the intended recipient nor the employee or > agent responsible for delivering this message to the intended recipient, you are > hereby notified that any disclosure, copying, distribution or the taking of any > action in reliance on the contents of this information is strictly prohibited. > If you have received this communication in error, please notify us immediately > by replying to the message and deleting it from your computer. Thank you. Saint > Joseph's Health System, Inc. From jnocito <@t> satx.rr.com Sat Mar 11 18:13:06 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sat Mar 11 18:11:10 2006 Subject: [Histonet] Help with Carnoy References: <270614B321ACB44D8C1D91F4F921FDC3036CBC06@NCH01EX02.nch.org> <002101c64508$82b25ce0$e8bd0b43@yourxhtr8hvc4p> <83AACDB0810528418AA106F9AE9B7F7E01825335@sjhaexc02.sjha.org> Message-ID: <001701c64569$bd74f840$e8bd0b43@yourxhtr8hvc4p> I buy mine from Poly Scientific ----- Original Message ----- From: "Weems, Joyce" To: "Joe Nocito" ; "Galiotto, Laura" ; Sent: Saturday, March 11, 2006 9:58 AM Subject: RE: [Histonet] Help with Carnoy > Where do you get your chloroform? > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Joe Nocito > Sent: Sat 3/11/2006 7:37 AM > To: Galiotto, Laura; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Help with Carnoy > > Laura, > I started using Carnoy's again because I was being told by my pathologists > that I wasn't retrieving all the lymph nodes in colon and breast cases. I > hope you have a well-ventilated area to work in because the fumes get > pretty > strong. We put the used Carnoy's in our alcohol/xylene waste containers to > be carried off. Good luck > > Joe Nocito BS, HT(ASCP)QIHC > Histology Manager > Pathology Reference Lab > San Antonio, TX > ----- Original Message ----- > From: "Galiotto, Laura" > To: > Sent: Friday, March 10, 2006 3:32 PM > Subject: [Histonet] Help with Carnoy > > > > Is anyone currently using Carnoy for fat dissolution in an attempt to > retrieve lymph nodes particularly in colon resections? If so pleases > advise > of process and disposal. The name of manufacturer or vender would also be > helpful. > Laura Galiotto, HT (ASCP) > Histology Facilitator > > Northwest Community Hospital > 800 West Central Road > Arlington Heights, Il 60005 > 847-618-6190 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.375 / Virus Database: 268.2.1/278 - Release Date: 3/9/2006 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > Confidentiality Notice ** The information contained in this message may be > privileged and is confidential information intended for the use of the > addressee listed above. If you are neither the intended recipient nor the > employee or agent responsible for delivering this message to the intended > recipient, you are hereby notified that any disclosure, copying, > distribution or the taking of any action in reliance on the contents of > this information is strictly prohibited. If you have received this > communication in error, please notify us immediately by replying to the > message and deleting it from your computer. Thank you. Saint Joseph's > Health System, Inc. > -------------------------------------------------------------------------------- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -------------------------------------------------------------------------------- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.375 / Virus Database: 268.2.1/279 - Release Date: 3/10/2006 From arunams <@t> interchange.ubc.ca Sat Mar 11 20:56:17 2006 From: arunams <@t> interchange.ubc.ca (arunams) Date: Sat Mar 11 20:56:23 2006 Subject: [Histonet] Help... ATPase staining Message-ID: <2812657.1142132177583.JavaMail.myubc2@portal9.itservices.ubc.ca> Hi All, I need some help with ATPase staining. Does any one have a good protocol? Mine use Sodium barbital with I can get from any where. Does any one know substitute or a protocol without Sodium barbital? Thanks for your help. Aruna From jcox90 <@t> yahoo.com Sun Mar 12 14:24:10 2006 From: jcox90 <@t> yahoo.com (Jill Cox) Date: Sun Mar 12 14:24:14 2006 Subject: [Histonet] Gram Stain kit Message-ID: <20060312202410.87544.qmail@web52102.mail.yahoo.com> Hello Histonetters, We just purchased a Ventana special stainer but it's not capable of performing a Gram stain. Does anyone know of a good kit to purchase??? Harleco was recommended but would still like to see what everyone else is using, thanks, jill From cwscouten <@t> myneurolab.com Sun Mar 12 17:19:48 2006 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Sun Mar 12 17:20:21 2006 Subject: [Histonet] vibratome sectioning of brain tissues Message-ID: <5784D843593D874C93E9BADCB87342AB01306BED@tpiserver03.Coretech-holdings.com> "Vibratome(TM)" is a registerd trademark of Vibratome company, formerly TPI. You have a vibrating blade microtome from another company, not a Vibratome. Thick Thin sections are most often caused by too shallow a blade angle. Steeper usually helps. However, thick thin can have other causes, such as the pedestal not rigidly locked to the instrument, or the blade not tightly locked down, or not a fresh sharp blade, or bad tissue quality. If you have tried blade angles to no avail, try grasping the tissue pedestal, then the blade carriage, and see if anything is loose or wobbles. If nothing interesting found, I would worry about tissue quality problems due to cooking the tissue in warm gelatin. Or the wash in tap water. Even partially fixed tissue can be damaged by tonicity challenges, although 25 hours in 4% formalin should mostly prevent that. But rinse in a volume of PBS instead. Many Vibratome users use no embedding. Glue the fixed brain to the pedestal, cutting a flat surface to glue down, and begin sectioning. The further from the pedestal you are cutting, the more flex in the system, and the more likely is thick thin, so try to block the brain down to the chunk you need by cutting off the ends far from the part you need. Let me know if any of this helps. Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bruijntjes, J.P. Sent: Friday, March 10, 2006 10:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] vibratome sectioning of brain tissues Hi all A colleague of me works with rat brains and tries to prepare vibratome section but with hardly no result. Main problem is thin/thick sections. This is what she has done with the tissues ect: Brain tissue: perfused with and fixed pre-embedding in 4% Formaline Embedding procedure: -during 24 H hemispheres were washed in running tap water -during the following 24 H hemispheres were infiltrated with gelatin (from bovine skin type B) solution, 12.5% w/w at 37? -during the next 24 H hemispsheres were infiltrated with gelatin, 25% w/w at 37? -after coagulation (approx 30 min) at 2-10 ? gelatin blocks were further fixed during 4 days in 4% formaline (2-10 ?). A (new) vibratome, HM 650 V, was used for sectioning. Intended section thickness: 35 ?m The buffertray was filled with PBS and crushed ice She have tried many different combinations of the following settings: -knife angle: 10-40 ? (approx) -frequency: 30-100 Hz -amplitude: 0.3-1.2 mm -speed: 1.5-5.0 mm/s She is running out of ideas. Suggestions are very welcome!!! Joost Bruijntjes TNO Quality of Life Toxicology and Applied Pharmacology Zeist Holland This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histology.bc <@t> shaw.ca Sun Mar 12 18:11:21 2006 From: histology.bc <@t> shaw.ca (Paul Bradbury) Date: Sun Mar 12 18:11:21 2006 Subject: [Histonet] Gram Stain kit and other stuff In-Reply-To: <20060312202410.87544.qmail@web52102.mail.yahoo.com> References: <20060312202410.87544.qmail@web52102.mail.yahoo.com> Message-ID: <4414B8A9.3090800@shaw.ca> I have a fundamental question to ask here. Why on earth would anyone want to automate Gram's stain? It is the simplest, quickest stain in the Histotechnologist's repertoire. It is also the cheapest stain to perform; less than $20.00 worth of reagents will last for years and stain hundreds of slides. Why buy an overpriced kit and then take up space on a machine that could be used for staining procedures that truly benefit from automation. One of last week's messages requested information about a supplier for Carnoy's fixative. Any laboratory worker can make Carnoy; it's not difficult! It's also a fraction of the price of the "commercial" Carnoy. Every other solution used in the Histology laboratory can be prepared for a fraction of the commercial cost. Another thread last week discussed the rising costs of procedures, billing, and the seemingly endless increases in health care expenditures. Yet, we seem to be becomimg more and more reliant on buying kits for every method and purchasing commercially-prepared reagents for every application. I am not advocating going back to doing everything manually, using home-made reagents, but I think we have to be more aware of the inflated costs involved in the use of automated techniques and commercially prepared reagents. Many hospitals or universities offer rewards to employees who suggest cost-saving ideas. You see where I am going with this? Just my thoughts for what they are worth, Paul Bradbury Kamloops, BC, Canada >Hello Histonetters, > We just purchased a Ventana special stainer but it's not capable of performing a Gram stain. Does anyone know of a good kit to purchase??? Harleco was recommended but would still like to see what everyone else is using, thanks, jill > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From kemlo.rogerson <@t> waht.swest.nhs.uk Mon Mar 13 02:31:16 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Mar 13 02:30:41 2006 Subject: [Histonet] Feelings Message-ID: I didn't put it there, the Pathology that is. Someone or something else did and we are now in the realms of faith (or lack of it!). Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 "Following the line of least resistance makes both men and rivers crooked" This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Kelsey Peek [mailto:kelseymichele@hotmail.com] Sent: Friday, March 10, 2006 9:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Feelings How does it make you feel when you find pathology in a tissue sample? Does it make you sad or are you just doing your job? _________________________________________________________________ Is your PC infected? Get a FREE online computer virus scan from McAfee(r) Security. http://clinic.mcafee.com/clinic/ibuy/campaign.asp?cid=3963 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike.kirby <@t> nhls.ac.za Mon Mar 13 08:08:51 2006 From: mike.kirby <@t> nhls.ac.za (Mike Kirby) Date: Mon Mar 13 08:05:41 2006 Subject: [Histonet] Use of cellular (mobile) phones in the Lab Message-ID: <452D0F6B16AA6E4092F6D3E9A9D97A620B7EBD@nhlsgpm002.NHLS.AC.ZA> Hi Guys. This is a slightly "off topic" query, but I need to draw up a policy on the use of cellular (mobile) phones in the Lab environment and would appreciate input from the Anatomical Pathology side. The main reasons for not using a phone are: 1. Danger of infection (primarily in the Micro/Virology Labs) 2. Interference with some electronic circuitry (primarily with the analytical units in the Biochemistry, Immunology & Haematology labs) 3. Interruption of the work flow and annoying to others (all labs). Is there any reason/s that you can think off pertaining to the Histopathology/Cytopathology labs? Thanks in advance. Mike Kirby National Health Lab Service Johannesburg South Africa. P.S. I'll accept replies from any Australians, that is if you are speaking to us after our one run win in the Cricket! (smirk, smirk!). ********************************************************************************** The views expressed in this email are, unless otherwise stated, those of the author and not those of the National Health Laboratory Services or its management. The information in this e-mail is confidential and is intended solely for the addressee. Access to this e-mail by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken or omitted in reliance on this, is prohibited and may be unlawful. Whilst all reasonable steps are taken to ensure the accuracy and integrity of information and data transmitted electronically and to preserve the confidentiality thereof, no liability or responsibility whatsoever is accepted if information or data is, for whatever reason, corrupted or does not reach its intended destination. *********************************************************************************** From Kathy.Johnston <@t> CLS.ab.ca Mon Mar 13 08:45:07 2006 From: Kathy.Johnston <@t> CLS.ab.ca (Kathy.Johnston@CLS.ab.ca) Date: Mon Mar 13 08:45:16 2006 Subject: [Histonet] Gram Stain kit Message-ID: <4BC300747AF87A48BCDF8E48BC2885CE013652E6@mail1.calgary.com> Our sites away from our special stains department are using the Brown and Hopps kit from Polyscientific for weekend on call usage and find it quite nice to use. I am using the same methodology on a regular basis in Sp Stains, but find that I go through too much reagent to use the kit. I prefer to make up my own. Kathy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jill Cox Sent: March 12, 2006 1:24 PM To: Histonet@Lists. Utsouthwestern. Edu Subject: [Histonet] Gram Stain kit Hello Histonetters, We just purchased a Ventana special stainer but it's not capable of performing a Gram stain. Does anyone know of a good kit to purchase??? Harleco was recommended but would still like to see what everyone else is using, thanks, jill _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify the sender immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. From rjbuesa <@t> yahoo.com Mon Mar 13 08:48:22 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 13 08:48:26 2006 Subject: [Histonet] Use of cellular (mobile) phones in the Lab In-Reply-To: <452D0F6B16AA6E4092F6D3E9A9D97A620B7EBD@nhlsgpm002.NHLS.AC.ZA> Message-ID: <20060313144822.20915.qmail@web61216.mail.yahoo.com> Hi Kirby: The essential reason I used to curtail (first) and elminate (later) the use of cell phones in the lab was the interruption of work flow. While at the bench they should have their cell phones "off" and their mesages to be transferred to their voice-mail feature. Those could be answered during break and lunch/dinner time. This is just a matter of definig/applying a policy that has to be followed. If there was an emergency the mesage could be related to the receptionist. There was some resistance at the beginning, but was accepted at the end. There were no cases of real emergencies and all adopted a message that said: "If this is an emergency call-----(and gave the operators switch board phone)" and this operator was instructed to contact the laboratory inmediately. Hope this will help you. Ren? J. Mike Kirby wrote: Hi Guys. This is a slightly "off topic" query, but I need to draw up a policy on the use of cellular (mobile) phones in the Lab environment and would appreciate input from the Anatomical Pathology side. The main reasons for not using a phone are: 1. Danger of infection (primarily in the Micro/Virology Labs) 2. Interference with some electronic circuitry (primarily with the analytical units in the Biochemistry, Immunology & Haematology labs) 3. Interruption of the work flow and annoying to others (all labs). Is there any reason/s that you can think off pertaining to the Histopathology/Cytopathology labs? Thanks in advance. Mike Kirby National Health Lab Service Johannesburg South Africa. P.S. I'll accept replies from any Australians, that is if you are speaking to us after our one run win in the Cricket! (smirk, smirk!). ********************************************************************************** The views expressed in this email are, unless otherwise stated, those of the author and not those of the National Health Laboratory Services or its management. The information in this e-mail is confidential and is intended solely for the addressee. Access to this e-mail by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken or omitted in reliance on this, is prohibited and may be unlawful. Whilst all reasonable steps are taken to ensure the accuracy and integrity of information and data transmitted electronically and to preserve the confidentiality thereof, no liability or responsibility whatsoever is accepted if information or data is, for whatever reason, corrupted or does not reach its intended destination. *********************************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Mail Use Photomail to share photos without annoying attachments. From DeBrosse_Beatrice <@t> Allergan.com Mon Mar 13 09:16:09 2006 From: DeBrosse_Beatrice <@t> Allergan.com (DeBrosse_Beatrice) Date: Mon Mar 13 09:16:45 2006 Subject: [Histonet] Gram Stain kit and other stuff Message-ID: Well said. Beatrice DeBrosse-Serra HT(ASCP)QIHC Allergan, Inc. 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5116 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paul Bradbury Sent: Sunday, March 12, 2006 4:11 PM To: HistoNet Server Subject: [Histonet] Gram Stain kit and other stuff I have a fundamental question to ask here. Why on earth would anyone want to automate Gram's stain? It is the simplest, quickest stain in the Histotechnologist's repertoire. It is also the cheapest stain to perform; less than $20.00 worth of reagents will last for years and stain hundreds of slides. Why buy an overpriced kit and then take up space on a machine that could be used for staining procedures that truly benefit from automation. One of last week's messages requested information about a supplier for Carnoy's fixative. Any laboratory worker can make Carnoy; it's not difficult! It's also a fraction of the price of the "commercial" Carnoy. Every other solution used in the Histology laboratory can be prepared for a fraction of the commercial cost. Another thread last week discussed the rising costs of procedures, billing, and the seemingly endless increases in health care expenditures. Yet, we seem to be becomimg more and more reliant on buying kits for every method and purchasing commercially-prepared reagents for every application. I am not advocating going back to doing everything manually, using home-made reagents, but I think we have to be more aware of the inflated costs involved in the use of automated techniques and commercially prepared reagents. Many hospitals or universities offer rewards to employees who suggest cost-saving ideas. You see where I am going with this? Just my thoughts for what they are worth, Paul Bradbury Kamloops, BC, Canada >Hello Histonetters, > We just purchased a Ventana special stainer but it's not capable of performing a Gram stain. Does anyone know of a good kit to purchase??? Harleco was recommended but would still like to see what everyone else is using, thanks, jill > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Susan.Ferrigon <@t> sanofi-aventis.com Mon Mar 13 09:17:52 2006 From: Susan.Ferrigon <@t> sanofi-aventis.com (Susan.Ferrigon@sanofi-aventis.com) Date: Mon Mar 13 09:18:06 2006 Subject: [Histonet] ICC -Kupffer cells Message-ID: Hi I am having trouble with staining of Kupffer cells, I am using Serotec F4/80 and have lots of help with protocols from some kind Histonetters, my question is, has anyone had good results with tissues which have been fixed for any length of time ( ie: 2 years). Thanks Susan ------------------------------ Le pr?sent message ainsi que ses ?ventuelles pi?ces jointes est exclusivement destin? au(x) destinataire(s), personnes physiques ou morales, qu'il d?signe. Il constitue de ce fait une correspondance ? caract?re priv? et peut contenir des informations confidentielles. Si ce message vous est parvenu par erreur, nous vous remercions d'en aviser imm?diatement l'exp?diteur par retour de ce courrier ?lectronique puis de le d?truire, ainsi que ses ?ventuellement pi?ces jointes, sans en conserver de copie. This message, including any attachment, is intended for the use of the individual or entity to which it is addressed. It is therefore to be considered as a private correspondence which may contain confidential information. If you are not the intended recipient, please advise the sender immediately by reply e.mail and delete this message and any attachment thereto without retaining a copy. ------------------------------ From PMonfils <@t> Lifespan.org Mon Mar 13 09:47:20 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Mon Mar 13 09:47:33 2006 Subject: [Histonet] Cell blocks, Message-ID: <09C945920A6B654199F7A58A1D7D1FDE0171768C@lsexch.lsmaster.lifespan.org> Here's my standard cell block technique, which I think is pretty simple: Get the cells into suspension in a centrifuge tube. Add a volume of 10% buffered formalin equal to the volume of the suspension (resulting in 5% buffered formalin) and mix by inversion or vortexing. Allow to fix for 10 minutes or longer. Add 1 drop of Mayer's albumin per ml of sample and mix by inversion or vortexing. Spin down and gently remove supernatant, by pipetting or pouring. Gently add 50% ethanol (hold the tube at a 45 degree angle and slowly pipette the alcohol onto the side of the tube, not directly onto the cells). Allow to stand 10 to 30 minutes, depending on the volume of the cell block. Pour off, replace with 70% ethanol. Allow to stand a similar time. Pour off, replace with 95% ethanol. Allow to stand a similar time. Remove cell block from centrifuge tube, wrap gently in lens paper, place in cassette, process and embed. The albumin coats the cells with a thin film of coagulable material, which subsequently coagulates in the alcohols, binding the cells together into a block. The 50% alcohol should be added carefully, so as not to cause turbulence that might bring some cells back into suspension. The 50% alcohol coagulates the albumin sufficiently so that the subsequent alcohols can be added less gently. Other proteinaceous material like gelatin or serum could substitute for albumin. The cell block shrinks somewhat during dehydration, just as tissue does, and usually comes loose from the bottom of the tube as a result. If it does not come loose on its own, just touching it with the tip of a probe will usually free it. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > Steven Coakley > Sent: Friday, March 10, 2006 1:07 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] Cell blocks, > > I'm looking for a way to make simple cell blocks. I work in research so I > need something that would cover routine cells and cell cultures?? > > Steve > > > --------------------------------- > Yahoo! Mail > Bring photos to life! New PhotoMail makes sharing a breeze. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From tkngflght <@t> yahoo.com Mon Mar 13 10:12:07 2006 From: tkngflght <@t> yahoo.com (Cheryl) Date: Mon Mar 13 10:12:12 2006 Subject: [Histonet] Using kits vs. making up your own In-Reply-To: <4414B8A9.3090800@shaw.ca> Message-ID: <20060313161207.30157.qmail@web50909.mail.yahoo.com> Just an FYI---Jill is a really good and efficient tech and a good steward. She's starting a new lab from the ground and they open--I think it's this week?!! Likely part of her reasons for picking up a kit is convenience. It comes ready to go, complete with a procedure so she can use the stain and come back around when time permits and redo everything for the best cost and time efficiency and stay within ASCP/JCAHO/NCCLS/OSHA (the list is endless) rules. She's been pedal to the metal for a couple of months and it comes together--is it today?! I totally agree, purchasing things that can be made quickly and easily is often more costly---and powder dyes last longer than made up reagent. We should also advocate recycling whenever possible. Most automated stainers are generally cost efficient and make the most of the tech time available.The greatest cost of making up one's own reagents and recycling is often wage for the tech. With the HUGE shortage of skilled techs, making up all of one's reagents, hand staining, and even setting up and maintaining a distiller is not always feasable. I'm glad someone noted the waste/expense of buying pre-made--but in Jill's defense--sometimes labs are in situations that warrant convenience over expense. Her situation is one-- at least until the lab is up and running! GO JILL!! Cheryl Kerry Full Staff Inc. Staffing the AP lab by helping one tech at a time. 281.852.9457 From judith_pardue <@t> memorial.org Mon Mar 13 11:28:11 2006 From: judith_pardue <@t> memorial.org (Pardue, Judith) Date: Mon Mar 13 11:28:16 2006 Subject: [Histonet] ATPase Message-ID: YOU CAN BUY SODIUM BARBITAL FROM SIGMA. This message and accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. "Sections 2510-2521", and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From arunams <@t> interchange.ubc.ca Mon Mar 13 11:47:57 2006 From: arunams <@t> interchange.ubc.ca (arunams) Date: Mon Mar 13 11:48:03 2006 Subject: [Histonet] ATPase Message-ID: <4161378.1142272077695.JavaMail.myubc2@portal9.itservices.ubc.ca> Hi there, Problem is that we need a health canada permit to buy sodium barbital in canada, which I don't have. Thanks aruna -----Original Message----- > Date: Mon Mar 13 09:28:11 PST 2006 > From: "Pardue, Judith" > Subject: [Histonet] ATPase > To: histonet@lists.utsouthwestern.edu > > YOU CAN BUY SODIUM BARBITAL FROM SIGMA. > > This message and accompanying documents are covered by the Electronic Communications Privacy Act, 18 U.S.C. "Sections 2510-2521", and contain information intended for the specified individual(s) only. This information is confidential. If you are not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, copying, or the taking of any action based on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cpgarcia <@t> salk.edu Mon Mar 13 12:38:26 2006 From: cpgarcia <@t> salk.edu (Carlos G. Perez-Garcia) Date: Mon Mar 13 12:38:03 2006 Subject: [Histonet] in situ hybridization Message-ID: Hi all, i am looking for a good protocol for ISH in Bouin's fixed tissue, can anybody suggest me one? best Carlos Carlos G. Perez-Garcia, Ph.D. Molecular Neurobiology Lab (MNL-O) The Salk Institute 10010 North Torrey Pines Road 92037 La Jolla, CA USA cpgarcia@salk.edu Fax: 858 558 6207 From gcallis <@t> montana.edu Mon Mar 13 13:12:53 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Mar 13 13:13:06 2006 Subject: [Histonet] H2DCFDA fading, photobleaching and antifade mounting medias Message-ID: <6.0.0.22.1.20060313115408.01b0fe20@gemini.msu.montana.edu> Glenn, You wrote: I am using H2DCFDA for the detection of cellular reactive oxygen species following treatment with various agents. Cells are subsequently mounted in anti-fadent with DAPI (Slow Fade Gold with DAPI). The green fluorescence appears awfully weak. I have noticed this in past experiments looking at cytochrome c release with FITC detection. Could the antifadent be the culprit? Don't some antifadent products quench fluorescein right-off-the-bat? Would it be best to just do a standard aqueous mount and be extra careful with light exposure? Reply: What you are experiencing is NOT quenching but photobleaching. FITC is far easier to photobleach when exposed to UV excitation and not performing the staining in the dark (under cover!) The mounting media helps prevent photobleaching but not 100%, however if you use Alexa 488 from Molecular Probes instead of FITC as the fluorophore, you will probably have far better retention of fluorescnce with the Alexa's. It definitely will be brighter and you can look at it longer with UV light. It is not only mounting media but also the choice of fluorophore. There are several mounting medias that Molecular Probes did a comparison on and showed(results available online)which mounting medias worked better for fluoresecence. You can learn a great deal about fluorsceinated fluorophores (FITC, TRITC, Texas Red) versus Alexa dyes that are sulfonated with the latter be superior. These are more more resistant to photobleaching and definitely worth a try. Molecular Probes Handbook explains this, although it is a rather "physical chemistry" oriented subject. The original publication in J HIstochem Cytochem by the Hauglands who developed the use of Alexa dyes is a good read. Microscopy Today just had a wonderful comparison of mounting medias for IFA by Collins, go back in Histonet Archives to access the website for this free article. I have never had a mounting media fade FITC immediately, but I do enjoy good fluroescent signal retention with this after using Molecular Probes hard set Prolong Gold ready to use with or without DAPI. I have use AquaMount, but examination of slide is same day - photobleaching generally occurs more at time of UV light excitation so we take photos at time of viewing sections. There are some ways to increase the signal - we use Strepavidin-Alex dyes with biotinylated secondary or a biotinylated primary to amplify more - Hope this helps. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From FDixon <@t> rd.us.loreal.com Mon Mar 13 16:40:17 2006 From: FDixon <@t> rd.us.loreal.com (DIXON Felicia) Date: Mon Mar 13 16:41:08 2006 Subject: [Histonet] Question Message-ID: I am having major problems sectioning human hair that is embed in nitrocellulose and paraffin. I have tried cutting with a glass knife and a low profile blade. I am working from a literature method but am new to embedding and mictrotomy. In addition to problems with cutting uniform cross-sections, I am losing a lot of fibers after clearing with xylenes and then oxidizing with performic acid. If anybody could give me suggestions on the best way to embed and cut hair then how to keep the cross-sections on the slide for treatment, I would greatly appreciate it. Felicia Dixon This message and any attachments (hereunder the « message ») are confidential and intended solely for the addressees. If you receive this message in error, please delete it and immediately notify the sender. If the reader of this message is not the intended recipient, you are hereby notified that any unauthorized use, copying or dissemination is prohibited. E-mails are susceptible to alteration. Neither L'OREAL nor any of its subsidiaries or affiliates shall be liable for the message if altered, changed or falsified. ============================================================================== From ploykasek <@t> phenopath.com Mon Mar 13 16:57:24 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Mon Mar 13 16:57:54 2006 Subject: [Histonet] Use of cellular (mobile) phones in the Lab In-Reply-To: <452D0F6B16AA6E4092F6D3E9A9D97A620B7EBD@nhlsgpm002.NHLS.AC.ZA> Message-ID: I have mixed feelings on the cell phone issue. So far, it has not been enough of an interruption to have to form a policy or limit it. It might be a matter of, do the employees have access to a phone & time to make personal phone calls? On another slightly "off topic" - how do people feel about the use of portable music devices by co-workers?? Patti Loykasek > Hi Guys. > > This is a slightly "off topic" query, but I need to draw up a policy on the > use of cellular (mobile) phones in the Lab environment and would appreciate > input from the Anatomical Pathology side. > > The main reasons for not using a phone are: > > 1. Danger of infection (primarily in the Micro/Virology Labs) > 2. Interference with some electronic circuitry (primarily with the analytical > units in the Biochemistry, Immunology & Haematology labs) > 3. Interruption of the work flow and annoying to others (all labs). > > Is there any reason/s that you can think off pertaining to the > Histopathology/Cytopathology labs? > > Thanks in advance. > > Mike Kirby > National Health Lab Service > Johannesburg > South Africa. > > P.S. I'll accept replies from any Australians, that is if you are speaking to > us after our one run win in the Cricket! (smirk, smirk!). > > ****************************************************************************** > **** > The views expressed in this email are, unless otherwise stated, those of the > author and not those of the National Health Laboratory Services or its > management. The information in this e-mail is confidential and is intended > solely for the addressee. > Access to this e-mail by anyone else is unauthorized. If you are not the > intended recipient, any disclosure, copying, distribution or any action taken > or omitted in reliance on this, is prohibited and may be unlawful. > Whilst all reasonable steps are taken to ensure the accuracy and integrity of > information and data transmitted electronically and to preserve the > confidentiality thereof, no liability or responsibility whatsoever is accepted > if information or data is, for whatever reason, corrupted or does not reach > its intended destination. > > ****************************************************************************** > ***** > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From tkngflght <@t> yahoo.com Mon Mar 13 18:45:09 2006 From: tkngflght <@t> yahoo.com (Cheryl) Date: Mon Mar 13 18:45:15 2006 Subject: [Histonet] Use of cellular (mobile) phones in the Lab In-Reply-To: Message-ID: <20060314004509.71885.qmail@web50911.mail.yahoo.com> I've been in a number of labs (close to 40!) and the policies were as different as each of the labs. Of course safety and analyser interference are of primary import--you may have to have a different set of rules by section for this reason. On break time people can do as they please as long as it doesn't interfere with the rest of the lab...but you want your people to be relaxed and comfortable at work. When I was in charge I always asked them as a group what they thought was fair...and worked from that. They most often came up with rules more strict than I would've!! (There will always be those who abuse the rules, but you know to reign them in in private and not punish the group for their choices.) As long as the lab phone is available to make and receive calls from kids and significant others, the school nurse, etc, we usually left personal phones in the lockers and only brought them into the lab when something important was going on (offer on a house, kid sick at home, family member in labor!), being sure to let everyone know a call was expected so no one got upset at the 'exception' to the rule. They were always encouraged to give out the lab phone for important stuff and everyone cooperated. As for MP3 players...this reduces the 'talk talk' between staff and lets more mistakes get through!! Can't overhear the pathologist asking a co-worker about something only YOU know the answer to when you have a headset on!! It also reduced the 'social' aspect between coworkers. Plug in and tune out--never thought it a good idea. I have worked in labs where everyone wore a headset--it was mostly because they didn't want to be there and didn't like each other!! I'd rather have the additional background noise of a communal radio (usually bought one GOOD one for each lab if they wanted one-I had to listen too!) and rotate days on who got to pick the station. As usual, this is my two cents...might not be the same for all. Rock on! Cheryl K. > Hi Guys. > > This is a slightly "off topic" query, but I need to draw up a policy on the > use of cellular (mobile) phones in the Lab environment and would appreciate > input from the Anatomical Pathology side. > > The main reasons for not using a phone are: > > 1. Danger of infection (primarily in the Micro/Virology Labs) > 2. Interference with some electronic circuitry (primarily with the analytical > units in the Biochemistry, Immunology & Haematology labs) > 3. Interruption of the work flow and annoying to others (all labs). > > Is there any reason/s that you can think off pertaining to the > Histopathology/Cytopathology labs? > > Thanks in advance. > > Mike Kirby > National Health Lab Service > Johannesburg > South Africa. > > P.S. I'll accept replies from any Australians, that is if you are speaking to > us after our one run win in the Cricket! (smirk, smirk!). > > ****************************************************************************** > **** > The views expressed in this email are, unless otherwise stated, those of the > author and not those of the National Health Laboratory Services or its > management. The information in this e-mail is confidential and is intended > solely for the addressee. > Access to this e-mail by anyone else is unauthorized. If you are not the > intended recipient, any disclosure, copying, distribution or any action taken > or omitted in reliance on this, is prohibited and may be unlawful. > Whilst all reasonable steps are taken to ensure the accuracy and integrity of > information and data transmitted electronically and to preserve the > confidentiality thereof, no liability or responsibility whatsoever is accepted > if information or data is, for whatever reason, corrupted or does not reach > its intended destination. > > ****************************************************************************** > ***** > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From conniegrubaugh <@t> hotmail.com Mon Mar 13 20:19:54 2006 From: conniegrubaugh <@t> hotmail.com (connie grubaugh) Date: Mon Mar 13 20:20:05 2006 Subject: [Histonet] Use of cellular (mobile) phones in the Lab In-Reply-To: Message-ID: On the cell phone issue. We were having problems with just a few people that would be on their cell phones all the time. So the rule is now that we can take incoming calls but they must be kept short and we must leave the area and either go to the break room or out of the lab. A few of us do not want to be without our phones and it is mainly that sometimes a family member needs to get in to contact with us immedately. As for ipods and such we all have them where I work and it is mainly because not everyone wants to listen to the same radio station or the same music. Mostly I do not want to listen to the complaining that is going on. I listen to books while I'm working. connie grubaugh

Connie G.

>From: Patti Loykasek >To: histonet >Subject: Re: [Histonet] Use of cellular (mobile) phones in the Lab >Date: Mon, 13 Mar 2006 14:57:24 -0800 > >I have mixed feelings on the cell phone issue. So far, it has not been >enough of an interruption to have to form a policy or limit it. It might be >a matter of, do the employees have access to a phone & time to make >personal phone calls? > >On another slightly "off topic" - how do people feel about the use of >portable music devices by co-workers?? > >Patti Loykasek > > > > Hi Guys. > > > > This is a slightly "off topic" query, but I need to draw up a policy on >the > > use of cellular (mobile) phones in the Lab environment and would >appreciate > > input from the Anatomical Pathology side. > > > > The main reasons for not using a phone are: > > > > 1. Danger of infection (primarily in the Micro/Virology Labs) > > 2. Interference with some electronic circuitry (primarily with the >analytical > > units in the Biochemistry, Immunology & Haematology labs) > > 3. Interruption of the work flow and annoying to others (all labs). > > > > Is there any reason/s that you can think off pertaining to the > > Histopathology/Cytopathology labs? > > > > Thanks in advance. > > > > Mike Kirby > > National Health Lab Service > > Johannesburg > > South Africa. > > > > P.S. I'll accept replies from any Australians, that is if you are >speaking to > > us after our one run win in the Cricket! (smirk, smirk!). > > > > >****************************************************************************** > > **** > > The views expressed in this email are, unless otherwise stated, those of >the > > author and not those of the National Health Laboratory Services or its > > management. The information in this e-mail is confidential and is >intended > > solely for the addressee. > > Access to this e-mail by anyone else is unauthorized. If you are not the > > intended recipient, any disclosure, copying, distribution or any action >taken > > or omitted in reliance on this, is prohibited and may be unlawful. > > Whilst all reasonable steps are taken to ensure the accuracy and >integrity of > > information and data transmitted electronically and to preserve the > > confidentiality thereof, no liability or responsibility whatsoever is >accepted > > if information or data is, for whatever reason, corrupted or does not >reach > > its intended destination. > > > > >****************************************************************************** > > ***** > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >------------------------------------------------------------------------- >This e-mail message, including any attachments, is for the sole use of >the intended recipients and may contain privileged information. Any >unauthorized review, use, disclosure or distribution is prohibited. If >you are not the intended recipient, please contact the sender by e-mail >and destroy all copies of the original message, or you may call PhenoPath >Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mbmphoto <@t> gmail.com Mon Mar 13 22:17:44 2006 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Mon Mar 13 22:17:18 2006 Subject: [Histonet] Erma large sledge microtme Message-ID: <3CB4B256-0379-4989-A7DA-19FAB0F594AA@gmail.com> We have in our neurological department an Erma Inc. large sledge (sliding) microtome cat # 08-100-4. Although I understand this microtome belongs to our department NO ONE knows how to use it, there is NO manual for this equipment and it looks like there are a few missing parts. Now, I have used a sliding microtome for the past 17 years & I have seen several different models, but I have not seen or heard of this name brand before. I have gone on the web to search for the company & found out that the instrument is made in Japan. So, if there is ANYONE out there who uses or has used this microtome - I would greatly appreciate it if you would contact me for information. This offers also goes for any vendors too. I look forward to hearing from anyone. Yours Maria Bartola Mejia University of California San Francisco (UCSF) Department of Neurological Surgery 1855 Folsom Street San Francisco, CA, 94103 Email: mbmphoto@gmail.com Phone: (415)-514-2954 From kemlo.rogerson <@t> waht.swest.nhs.uk Tue Mar 14 02:16:57 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Mar 14 02:16:16 2006 Subject: [Histonet] Use of cellular (mobile) phones in the Lab Message-ID: I gather that the analogue phones were the ones implicated in causing problems; as far as I am aware the digital ones don't unless they are in the very close vicinity of sensitive equipment. Here it is not allowed to have your phones on as Intensive Care is above our heads, literally. That phones addle your brain is another matter and looking at some of our youngsters I wonder. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 "Following the line of least resistance makes both men and rivers crooked" This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Patti Loykasek [mailto:ploykasek@phenopath.com] Sent: Monday, March 13, 2006 10:57 PM To: histonet Subject: Re: [Histonet] Use of cellular (mobile) phones in the Lab I have mixed feelings on the cell phone issue. So far, it has not been enough of an interruption to have to form a policy or limit it. It might be a matter of, do the employees have access to a phone & time to make personal phone calls? On another slightly "off topic" - how do people feel about the use of portable music devices by co-workers?? Patti Loykasek > Hi Guys. > > This is a slightly "off topic" query, but I need to draw up a policy on the > use of cellular (mobile) phones in the Lab environment and would appreciate > input from the Anatomical Pathology side. > > The main reasons for not using a phone are: > > 1. Danger of infection (primarily in the Micro/Virology Labs) > 2. Interference with some electronic circuitry (primarily with the analytical > units in the Biochemistry, Immunology & Haematology labs) > 3. Interruption of the work flow and annoying to others (all labs). > > Is there any reason/s that you can think off pertaining to the > Histopathology/Cytopathology labs? > > Thanks in advance. > > Mike Kirby > National Health Lab Service > Johannesburg > South Africa. > > P.S. I'll accept replies from any Australians, that is if you are speaking to > us after our one run win in the Cricket! (smirk, smirk!). > > **************************************************************************** ** > **** > The views expressed in this email are, unless otherwise stated, those of the > author and not those of the National Health Laboratory Services or its > management. The information in this e-mail is confidential and is intended > solely for the addressee. > Access to this e-mail by anyone else is unauthorized. If you are not the > intended recipient, any disclosure, copying, distribution or any action taken > or omitted in reliance on this, is prohibited and may be unlawful. > Whilst all reasonable steps are taken to ensure the accuracy and integrity of > information and data transmitted electronically and to preserve the > confidentiality thereof, no liability or responsibility whatsoever is accepted > if information or data is, for whatever reason, corrupted or does not reach > its intended destination. > > **************************************************************************** ** > ***** > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Tue Mar 14 04:05:23 2006 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Tue Mar 14 04:06:23 2006 Subject: [Histonet] Use of cellular (mobile) phones in the Lab In-Reply-To: <452D0F6B16AA6E4092F6D3E9A9D97A620B7EBD@nhlsgpm002.NHLS.AC.ZA> Message-ID: <000001c6474e$cfa3d680$503dd445@HPPav2> In our hospital, administration has stated exactly WHERE cell phones can be used (main lobbies, cafeterias, outside). Everywhere else, the cell phones are not allowed. They must be in the OFF position, not just in the stand-by mode. The hospital had our biomed people testing all floors and all areas, to see where the cell phones interfered with electronic circuitry, especially the heart telemetry which is set up all over the hospital (so patients being monitored can walk outside or go to the library, etc.). Then the hospital just made it uniform for everyone (in other words, it didn't matter if there was no interference on the 6th floor, but there was on the 7th floor, the only place either floor could use it was lobbies, outside, etc.). Makes it easier to enforce, and less confusing for visitors and patients. It looks like you might be working in a private lab, that doesn't have a policy to begin with. I thought I would mention it to those working in hospitals, to check what the policy of their institution is. (P.S. The same applies to personal pagers. They are to be in the OFF position. There are phones in the labs, if there is a family emergency.) Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Kirby Sent: Monday, March 13, 2006 9:09 AM To: Histonet (E-mail) Subject: [Histonet] Use of cellular (mobile) phones in the Lab Hi Guys. This is a slightly "off topic" query, but I need to draw up a policy on the use of cellular (mobile) phones in the Lab environment and would appreciate input from the Anatomical Pathology side. The main reasons for not using a phone are: 1. Danger of infection (primarily in the Micro/Virology Labs) 2. Interference with some electronic circuitry (primarily with the analytical units in the Biochemistry, Immunology & Haematology labs) 3. Interruption of the work flow and annoying to others (all labs). Is there any reason/s that you can think off pertaining to the Histopathology/Cytopathology labs? Thanks in advance. Mike Kirby National Health Lab Service Johannesburg South Africa. P.S. I'll accept replies from any Australians, that is if you are speaking to us after our one run win in the Cricket! (smirk, smirk!). **************************************************************************** ****** The views expressed in this email are, unless otherwise stated, those of the author and not those of the National Health Laboratory Services or its management. The information in this e-mail is confidential and is intended solely for the addressee. Access to this e-mail by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken or omitted in reliance on this, is prohibited and may be unlawful. Whilst all reasonable steps are taken to ensure the accuracy and integrity of information and data transmitted electronically and to preserve the confidentiality thereof, no liability or responsibility whatsoever is accepted if information or data is, for whatever reason, corrupted or does not reach its intended destination. **************************************************************************** ******* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Tue Mar 14 04:43:49 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Tue Mar 14 04:43:58 2006 Subject: [Histonet] Gram Stain kit Message-ID: Jill: The Brown and Brenn kit from PolyScientific works very well for us. www.polyrnd.com Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jill Cox Sent: Sunday, March 12, 2006 3:24 PM To: Histonet@Lists. Utsouthwestern. Edu Subject: [Histonet] Gram Stain kit Hello Histonetters, We just purchased a Ventana special stainer but it's not capable of performing a Gram stain. Does anyone know of a good kit to purchase??? Harleco was recommended but would still like to see what everyone else is using, thanks, jill _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Tue Mar 14 06:23:07 2006 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Tue Mar 14 06:23:17 2006 Subject: [Histonet] fish histology Message-ID: Anyone know of a fish histology/histopathology website???I am aware of the fathead minnow histology site, which is pretty good but has a number of gaps. Many thanks Richard Edwards MRC TOXICOLOGY UNIT LEICESTER. U.K.... From MSafron <@t> wilresearch.com Tue Mar 14 08:44:04 2006 From: MSafron <@t> wilresearch.com (Mike Safron) Date: Tue Mar 14 08:45:38 2006 Subject: [Histonet] Leica IPS Slide label system Message-ID: Does anybody have feedback, positive or negative, regarding the Leica IPS Slide label system. We are looking at purchasing this to replace out our current slide etcher. Please email any comments to msafron@wilresearch.com Michael Safron A.S.,HT(ASCP) Manager, Histology WIL Research Laboratories LLC 419-289-8700 ext: 3040 Ashland, Ohio 44805 From lesley.bechtold <@t> jax.org Tue Mar 14 09:41:00 2006 From: lesley.bechtold <@t> jax.org (Lesley Bechtold) Date: Tue Mar 14 09:43:16 2006 Subject: [Histonet] Lac Z automated stainer Message-ID: <20060314104100880.00000003156@spikey> Hi Histonetters! Does anyone know of a stainer that does a good job automating Lac Z staining (on slides)? And if you do know one, approximately how much does it cost? Thanks in advance! Lesley Lesley S. Bechtold Senior Manager, Histopathology & Microscopy Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 From O.M.Gallagher <@t> sheffield.ac.uk Tue Mar 14 10:30:44 2006 From: O.M.Gallagher <@t> sheffield.ac.uk (Orla Gallagher) Date: Tue Mar 14 10:31:10 2006 Subject: [Histonet] Aqueous mountant for fluorescence work Message-ID: <4416EFB4.27186.175CD4C@localhost> Dear Histonet, Does anyone know of an aqueous mounting medium which doesn't fade fluorescently-labelled cell preparations and which sets hard for convenient storage? Thanks, Orla ****************************** Ms.Orla Gallagher Academic Unit of Bone Biology Division of Clinical Sciences (South) D Floor (DU20) Medical School Henry Wellcome Laboratories for Medical Research University of Sheffield Beech Hill Road Sheffield S10 2RX www.sheffield.ac.uk E-mail:o.m.gallagher@sheffield.ac.uk Tel: 0114 271 3783(lab) 0114 271 3954(office) Fax: 0114 271 1711 From dennijc <@t> vetmed.auburn.edu Tue Mar 14 10:30:23 2006 From: dennijc <@t> vetmed.auburn.edu (John C. Dennis) Date: Tue Mar 14 10:31:15 2006 Subject: [Histonet] Vectashield Hardset Mountant Message-ID: <5.1.1.5.1.20060314102747.02422e50@mailhost.vetmed.auburn.edu> Dear all: Has anyone tried Vectashield Hardset? Good results? I work in Alabama where the humidity is pretty good most days and I notice that this stuff is water soluble. A potential problem? Your advice and counsel will be appreciated. Thanks, John C. Dennis From gcallis <@t> montana.edu Tue Mar 14 11:07:57 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Mar 14 11:08:08 2006 Subject: [Histonet] Aqueous mountant for fluorescence work In-Reply-To: <4416EFB4.27186.175CD4C@localhost> References: <4416EFB4.27186.175CD4C@localhost> Message-ID: <6.0.0.22.1.20060314100422.01b3b020@gemini.msu.montana.edu> Molecular Probes Prolong Gold antifade ready to use, a hard set. You still need to seal it but do this with diluted permanent mounting media (use the same solvent contained in the mounting media). If you do NOT seal it, it will retract over time. Fingernail polish is not ideal if GFP is involved, it can cause the GFP to lose fluorescence. Vector Laboratories makes a hard set, Vectashield Hard Set. Both companies also have these mounting medias with DAPI if you wish to have your nuclei stained. At 09:30 AM 3/14/2006, you wrote: >Dear Histonet, > >Does anyone know of an aqueous mounting medium which doesn't >fade fluorescently-labelled cell preparations and which sets hard for >convenient storage? > >Thanks, >Orla >****************************** > >Ms.Orla Gallagher >Academic Unit of Bone Biology >Division of Clinical Sciences (South) >D Floor (DU20) Medical School >Henry Wellcome Laboratories for Medical Research >University of Sheffield >Beech Hill Road >Sheffield S10 2RX >www.sheffield.ac.uk > >E-mail:o.m.gallagher@sheffield.ac.uk >Tel: 0114 271 3783(lab) > 0114 271 3954(office) >Fax: 0114 271 1711 > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From dpahisto <@t> yahoo.com Tue Mar 14 11:46:45 2006 From: dpahisto <@t> yahoo.com (Cindy DuBois) Date: Tue Mar 14 11:46:49 2006 Subject: [Histonet] Education requirements Message-ID: <20060314174645.53997.qmail@web33401.mail.mud.yahoo.com> I was curious whatever happened with the proposed continuing education requirements to maintain ASCP HT certification? Was it implemented and if so what are the terms? We are having a harder time getting our lab director to pay for such things as the NSH teleconferences and the society meetings. But if this was a requirement to maintain licensure, I am sure it would not be an issue. Cindy DuBois, HT ASCP Integrated Pathology --------------------------------- Yahoo! Mail Use Photomail to share photos without annoying attachments. From jhaviland <@t> mdanderson.org Tue Mar 14 12:01:05 2006 From: jhaviland <@t> mdanderson.org (jhaviland@mdanderson.org) Date: Tue Mar 14 12:01:12 2006 Subject: [Histonet] Joie C. Haviland/MDACC is out of the office. Message-ID: I will be out of the office starting 03/14/2006 and will not return until 03/17/2006. I will respond to your message when I return. From kmilne <@t> bccancer.bc.ca Tue Mar 14 12:11:33 2006 From: kmilne <@t> bccancer.bc.ca (Milne, Katy) Date: Tue Mar 14 12:11:39 2006 Subject: [Histonet] F4/80 vs CD68 Message-ID: <07979E76B0869D4E8C9FE4AA9FC065780A64B4@srvex03.phsabc.ehcnet.ca> Hi everyone, I'm aiming to look at macrophages in formalin-fixed paraffin-embedded mouse tumour tissue, I've been looking at the archives and there seems to be a lot of people that swear by F4/80 and others that are with the CD68 camp. I'm wondering if there is a specific reason to use one over the other or if they are interchangeable and it's just a matter of finding an antibody that works well. If anyone knows why or if one is better that the other in terms of expression levels on different types of macrophages, your input would be greatly appreciated. Thanks, Katy Milne Deeley Research Centre, BC Cancer Agency, 2410 Lee Avenue, Victoria BC V8R6V5 From emerald_lake77 <@t> yahoo.com Tue Mar 14 13:11:12 2006 From: emerald_lake77 <@t> yahoo.com (GT Hebert) Date: Tue Mar 14 13:11:16 2006 Subject: [Histonet] F4/80 vs CD68 In-Reply-To: <07979E76B0869D4E8C9FE4AA9FC065780A64B4@srvex03.phsabc.ehcnet.ca> Message-ID: <20060314191112.50112.qmail@web31704.mail.mud.yahoo.com> Hello Katy, In my experience, I have gotten Serotec's F4/80 antibody to work with FFPE mouse tissue ONLY within spleen and liver samples. However, when trying to detect macrophages within mouse aortic sinus lesions, I am unable to get positive results. I switched to CD68 and got excellent results. I have used many different fixatives (PLP, 4% PF, 10%NBF) and different retrieval methods for F4/80 and (contrary to literature) have not been able to get decent positive results -- except for those listed as positive controls, namely cells within the spleen and the Kupffer cells in liver. I look forward to other people's responses. Gustave T. Hebert Scientist II Cardiovascular and Metabolic Diseases Wyeth Research Cambridge MA "Milne, Katy" wrote: Hi everyone, I'm aiming to look at macrophages in formalin-fixed paraffin-embedded mouse tumour tissue, I've been looking at the archives and there seems to be a lot of people that swear by F4/80 and others that are with the CD68 camp. I'm wondering if there is a specific reason to use one over the other or if they are interchangeable and it's just a matter of finding an antibody that works well. If anyone knows why or if one is better that the other in terms of expression levels on different types of macrophages, your input would be greatly appreciated. Thanks, Katy Milne Deeley Research Centre, BC Cancer Agency, 2410 Lee Avenue, Victoria BC V8R6V5 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. --------------------------------- Yahoo! Mail Bring photos to life! New PhotoMail makes sharing a breeze. From TJJ <@t> Stowers-Institute.org Tue Mar 14 13:14:15 2006 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Tue Mar 14 13:14:31 2006 Subject: [Histonet] Re: Vectashield Hardset Mountant Message-ID: John, we tried it, didn't like it. I found that it didn't spread well, and when dry would leave huge air pockets or air bubbles. I could never really put "enough" mounting medium on...any more and the coverslip would have slid around out of place. We switched to the ProLong Gold, anti-fade. It's expensive, but given the fact we're a service facility, it's worth it to use the right tool for the job for our researchers. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64133 From NMargaryan <@t> childrensmemorial.org Tue Mar 14 13:15:48 2006 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Tue Mar 14 13:14:52 2006 Subject: [Histonet] Keratin and Vimentin Message-ID: <63B8B599DE283148B92E83C78B32C15D01C76869@cmhexbe2.childrensmemorial.org> Hi Histoland, I need to stain (IHC) mouse paraffin-embedded formalin fixed tissue for Keratin and Vimentin antibody. Could you, please suggest me a good Ab and send a protocol? Thanks in advance, Naira Naira V. Margaryan, D.V.M., Ph.D. Research Associate III Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3394 Tel: 773-755-6570/ext-8 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 From cfranci <@t> rigel.com Tue Mar 14 13:16:16 2006 From: cfranci <@t> rigel.com (Christian Franci) Date: Tue Mar 14 13:16:23 2006 Subject: [Histonet] Frozen sections Message-ID: greetings and salutations! Recently, I've had problems with my tissues sticking to the slides. It's quite possible that I got a bad batch of slides that aren't as charged as they should be. Since I got so many samples already sectioned, does anyone have any pointers or tricks so that I can use the samples that are ready to be stained without watching them peel off? Thanks Chris From rgrow <@t> bmnet.com Tue Mar 14 13:19:20 2006 From: rgrow <@t> bmnet.com (rgrow@bmnet.com) Date: Tue Mar 14 13:19:27 2006 Subject: [Histonet] Re: Pathology Programs In-Reply-To: Message-ID: WindoPath by Psyce Systems has performed well for our pathology department. Renee Grow, BA., HT (ASCP) rgrow@bmnet.com Histology Supervisor Blount Memorial Hospital 907 E. Lamar Alexander Pkwy. Maryville, TN 37804-5016 (865) 977-4744 (865) 977-5766 Fax histonet-request@ lists.utsouthwest ern.edu To Sent by: histonet@lists.utsouthwestern.edu histonet-bounces@ cc lists.utsouthwest ern.edu Subject Histonet Digest, Vol 28, Issue 19 03/11/2006 01:02 PM Please respond to histonet@lists.ut southwestern.edu Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Pathology Programs (poru715@comcast.net) ---------------------------------------------------------------------- Message: 1 Date: Sat, 11 Mar 2006 17:46:08 +0000 From: poru715@comcast.net Subject: RE: [Histonet] Pathology Programs To: "Weems, Joyce" , "Parker, Helayne" , Message-ID: <031120061746.16857.44130 CE00004E43A000041D92207021053CACEC99A9D019F@comcast.net> Content-Type: text/plain; charset="us-ascii" Copath by far........we have 35K surgicals per year with over 20K cyto. It is also easy to apply other computer programs such as with slide etchers because of easy compatibility. I've used other systems such as Sunquest and Meditech but Copath seems to be the best for the overall Anatomic Path department (ex. cyto, histo, pathologists, and clerical). -------------- Original message -------------- From: "Weems, Joyce" > I'm thinking you are asking about computer programs? > There are several - Copath the best that I have heard of - designed by a > pathologist so it is good for anatomic path. Although I have not used it, I hear > good things about it. > > I am currently using Triple G, which I cannot highly recommend. > > Good luck, > Joyce > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Parker, Helayne > Sent: Fri 3/10/2006 4:26 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Pathology Programs > > Hi Gang, > I was just wondering if anyone has any input on a good Pathology > Program out there. One that is user friendly and good for the clerical > people, technical people and MOST IMPORTANTLY the Pathologist. We do > about 10K surgical cases a year and 500 cytology cases. > > Helayne Parker, HT (A.S.C.P.) > Histology Section Head > Skaggs Community Health Center > Branson, Missouri > hparker@skaggs.net > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > Confidentiality Notice ** The information contained in this message may be > privileged and is confidential information intended for the use of the addressee > listed above. If you are neither the intended recipient nor the employee or > agent responsible for delivering this message to the intended recipient, you are > hereby notified that any disclosure, copying, distribution or the taking of any > action in reliance on the contents of this information is strictly prohibited. > If you have received this communication in error, please notify us immediately > by replying to the message and deleting it from your computer. Thank you. Saint > Joseph's Health System, Inc. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 28, Issue 19 **************************************** From TJJ <@t> Stowers-Institute.org Tue Mar 14 13:20:46 2006 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Tue Mar 14 13:21:00 2006 Subject: [Histonet] Re: in situ hybridization (bouin's fix) Message-ID: Carlos, I have not performed ISH on Bouin's fixed samples yet. Frankly, I think it's a good idea to stay away from Bouins because of the acetic acid in the fixative. Most ISH protocols warn against using acid decalcifiers on samples slated for ISH. If that is all you have to work with, I would use a standard ISH protocol and make no modifications and see if you get any result. Make sure you use sense and anti-sense probe and compare the two. Make sure you use a sample known to be positive for your target of interest. Are you using DNA or RNA probes, and is your target cellular DNA, cellular RNA, or viral? Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64133 From clarissabush <@t> sbcglobal.net Tue Mar 14 13:24:09 2006 From: clarissabush <@t> sbcglobal.net (CM Bush) Date: Tue Mar 14 13:24:14 2006 Subject: [Histonet] Would like to post histo job. Message-ID: <20060314192409.55741.qmail@web80332.mail.yahoo.com> Dear Histonet, We would like to post a histotechnician job opening on Histonet. Just wanted to make sure it is alright to do so here. Thank you very much. Clarissa Bush From liz <@t> premierlab.com Tue Mar 14 13:38:09 2006 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Tue Mar 14 13:38:14 2006 Subject: [Histonet] F4/80 vs CD68 In-Reply-To: <07979E76B0869D4E8C9FE4AA9FC065780A64B4@srvex03.phsabc.ehcnet.ca> Message-ID: <000001c6479e$d30bf960$c6d48a80@Chlipala> Katy I have used both F4/80 and CD68 antibodies from serotec for mouse macrophages. F4/80 detects a protein that is expressed by murine macrophages. This antibody binds to macrophages from different sites including the peritoneal caviety, lung, spleen, and thymus, to blood monocytes and to macrophages derived from bone marrow precursors in culture. This antigen is not expressed by lymphocytes or polymorphonuclear cells. This antibody will also stain kupfer cells in the liver. Mouse bone marrow should be approximately 30% positive. If you look at references regarding the presence of F4/80 you will find that expression of the protein changes dependent upon macrophage activation. This antibody will not detect aveolar macrophages, but I have seen it stain macrophages in the TB infected mouse lung. I consider it more of a activated macrophage marker and have used it for both frozen and paraffin sections. The rat anti-mouse CD68 from serotec is directed against macrosialin a heavily glycosylated transmembrane protein that is specifically expressed by tissue macrophages, langerhans cells and at low levels by dendritic cells. This antibody will detect aveolar macrophages. As far as GT's comments I have not experienced that with F4/80 especially if he is looking at aortic root sections in mouse. I have used both CD68 and F4/80 to detect macrophages in mouse aortic root samples. We routinely use F4/80 to stain for macrophages in tumor xenografts. I have protocols and images of both. GT I have some images of both F4/80 and CD68 in mouse aortic root sections. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Milne, Katy Sent: Tuesday, March 14, 2006 11:12 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] F4/80 vs CD68 Hi everyone, I'm aiming to look at macrophages in formalin-fixed paraffin-embedded mouse tumour tissue, I've been looking at the archives and there seems to be a lot of people that swear by F4/80 and others that are with the CD68 camp. I'm wondering if there is a specific reason to use one over the other or if they are interchangeable and it's just a matter of finding an antibody that works well. If anyone knows why or if one is better that the other in terms of expression levels on different types of macrophages, your input would be greatly appreciated. Thanks, Katy Milne Deeley Research Centre, BC Cancer Agency, 2410 Lee Avenue, Victoria BC V8R6V5 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1.1442 (20060314) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From akbitting <@t> geisinger.edu Tue Mar 14 13:56:28 2006 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Tue Mar 14 13:57:02 2006 Subject: [Histonet] Ventana Special Stains Message-ID: I'm looking for someone who is doing their PAS stains with the Ventana Special Stainer. I'm not happy with the way my liver bxs are looking and am looking for some advice. Thanks in advance. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From tfranzod <@t> dal.ca Tue Mar 14 14:05:41 2006 From: tfranzod <@t> dal.ca (Tamara Franz-Odendaal) Date: Tue Mar 14 14:06:00 2006 Subject: [Histonet] alkaline phosphatase in cartilage Message-ID: <00e101c647a2$b15d17a0$6d20ad81@tam> I am looking for a protocol to do Alkaline phosphatase staining of cartilage in 2cm fish by whole-mount. I have a protocol for sections and am wondering how much longer I need to stain to be sure that staining has penetrated everywhere and also whether I need to replace the solution every few hours. Anyone have any insights? Thanks Tamara Dr. Tamara Franz-Odendaal Post-doctoral fellow Biology Dept., Dalhousie University Halifax, NS, B3H 4J1 Canada From Yves.Heremans <@t> vub.ac.be Tue Mar 14 14:25:10 2006 From: Yves.Heremans <@t> vub.ac.be (Yves Heremans) Date: Tue Mar 14 14:25:17 2006 Subject: [Histonet] double/triple staining with streptavidin-Alexa Fluor Message-ID: <20060314202510.3C8828D01@mach.vub.ac.be> Dear HistoNetters, Is there a way to perform double (or triple) staining using secondary, biotinylated antibodies with streptavidin-Alexa Fluor conjugates, for instance, anti-rabbit, biotinylated secondary antibody A, detected with streptavidin-AF488 in combination with anti-mouse, biotinylated secondary antibody B, detected with streptavidin-AF555, preventing cross-reaction ? Will sequential staining (i.e. first anti-rabbit and SA488, then anti-mouse and SA555) saturate all biotin binding sites from the first step ? Yves From ana.merino-trigo <@t> wanadoo.fr Tue Mar 14 14:31:00 2006 From: ana.merino-trigo <@t> wanadoo.fr (ana.merino-trigo) Date: Tue Mar 14 14:31:18 2006 Subject: [Histonet] please unsubscribe References: <20060314202510.3C8828D01@mach.vub.ac.be> Message-ID: <02da01c647a6$364d80f0$5b790a0a@BABA> Please unsubscribe, Thanks everyone for everything that I learnt with you Ana From Melissa.Gonzalez <@t> cellgenesys.com Tue Mar 14 14:37:26 2006 From: Melissa.Gonzalez <@t> cellgenesys.com (Melissa Gonzalez) Date: Tue Mar 14 14:37:38 2006 Subject: [Histonet] Automated Slide Labeler Message-ID: Dear all, We are looking into purchasing an automatic slide etcher/labeler to label slides. I was hoping to get some feedback about brands/ cost and how happy people are with theirs. Thanks in advance, Melissa From jkiernan <@t> uwo.ca Tue Mar 14 14:49:43 2006 From: jkiernan <@t> uwo.ca (John A. Kiernan) Date: Tue Mar 14 14:49:45 2006 Subject: [Histonet] Re: in situ hybridization (bouin's fix) References: Message-ID: <44172C67.2A07FAAD@uwo.ca> That's right. Picric acid removes some of the bases from DNA, exposing deoxyribose units, which can react as aldehydes. It's not a full-blown Feulgen hydrolysis, but it's going that way. Hybridization histochemistry requires the purine and pyrimidine bases of the DNA in the tissue to be all present and correct, to pair with those of the probe. If you're using in situ hybridization to detect messenger RNA, then Bouin's may well be even more destructive because acid hydrolysis breaks RNA into soluble fragments. John Kiernan Anatomy, UWO London, Canada ------------------------------ "Johnson, Teri" wrote: > > Carlos, > > I have not performed ISH on Bouin's fixed samples yet. Frankly, I think > it's a good idea to stay away from Bouins because of the acetic acid in > the fixative. Most ISH protocols warn against using acid decalcifiers on > samples slated for ISH. If that is all you have to work with, I would > use a standard ISH protocol and make no modifications and see if you get > any result. Make sure you use sense and anti-sense probe and compare > the two. Make sure you use a sample known to be positive for your > target of interest. > > Are you using DNA or RNA probes, and is your target cellular DNA, > cellular RNA, or viral? > > Teri Johnson, HT(ASCP)QIHC > Managing Director Histology Facility > Stowers Institute for Medical Research > 1000 E. 50th St. > Kansas City, MO 64133 > From mward <@t> wfubmc.edu Tue Mar 14 14:51:07 2006 From: mward <@t> wfubmc.edu (Martha Ward) Date: Tue Mar 14 14:52:27 2006 Subject: [Histonet] Renal biopsies Message-ID: <61135F0455D33347B5AAE209B903A30412E645F8@EXCHVS2.medctr.ad.wfubmc.edu> I want to get a feel for how others receiving renal biopsies handle specimens from outside their institution. Currently outside clients divide the biopsy and submit it to us in 10% NBF, Zeus transport media for IMF and 2.5% gluteraldehyde for EM. One of our outside physicians wants to perform the biopsy at a neighboring hospital (located about a mile from us), have it packaged for transport to us by their pathology department and sent to us on moistened gauze for us to assess and divide appropriately. (This arises from some situations where the material was not divided optimally for the various procedures.) I have reservations about this but I would like to hear how other institutions handle this. Thanks in advance for your help with this. You can respond to me directly or to the whole group. Martha Ward, MT (ASCP) QIHC Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 From gcallis <@t> montana.edu Tue Mar 14 15:13:49 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Mar 14 15:14:00 2006 Subject: [Histonet] double/triple staining with streptavidin-Alexa Fluor In-Reply-To: <20060314202510.3C8828D01@mach.vub.ac.be> References: <20060314202510.3C8828D01@mach.vub.ac.be> Message-ID: <6.0.0.22.1.20060314135243.01b41140@gemini.msu.montana.edu> Yes, however, using an avidin/biotin blocks before each antibody to ensure 1) quenching endog biotin before first and quenching any residual biotin left over from first antibody sequence before you do the second primary sequence. We do double IFA staining with two rat antimouse monoclonals in the following way with one purified monoclonal and one biotinylated monoclonal primary. Frozen sections fixed with acetone alcohol mixture at RT after overnight drying Normal serum block matched to host of secondary used Strepavidin/biotin block (Vector) Rat antiMouse monoclonal Donkey antiRat F(ab')2 frag of IgG conjugated to RRX Rat serum block, 5% for 15 minutes Primary #2 rat antiMouse-biotinylated Strepavidin-Alexa 488 Prolong gold antifade with OR without DAPI If we do something like an Goat anti GFP/Donkey antigoat-FITC and Rat antiMouse CD11c-biotinylated/Strepavidin-Alexa 555, we shorten our protocol time considerably. A cocktail of Goat antiGFP and Rat antiMouse CD11c, followed by Dk antiGoat secondary-FITC, and then SA-488. This gets rid of so much sequential staining and blocking because that is all done in the beginning with Normal serum and the avidin/biotin blocking. To do this, "Will sequential staining (i.e. first anti-rabbit and SA488, then anti-mouse and SA555) saturate all biotin binding sites from the first step ?" I would advise doing either Strepavidin/biotin block before each primary (one could use Avidin/biotin block) particularly before the second primary to ensure saturation of biotin binding sites. We prefer to do a secondary conjugated with fluorophore for one of the antibodies, and make sure your secondaries ARE adsorbed to the species being stained and do NOT cross react in any way. Some people have success using secondaries conjugated to Alexa fluorophores (you can do your own conjugations with their kits) and not end up with so much Strepavidin fluorophores in the protocol which makes for a LONG day of staining. At 01:25 PM 3/14/2006, you wrote: >Dear HistoNetters, > >Is there a way to perform double (or triple) staining using secondary, >biotinylated antibodies with streptavidin-Alexa Fluor conjugates, for >instance, anti-rabbit, biotinylated secondary antibody A, detected with >streptavidin-AF488 in combination with anti-mouse, biotinylated secondary >antibody B, detected with streptavidin-AF555, preventing cross-reaction ? >Will sequential staining (i.e. first anti-rabbit and SA488, then >anti-mouse and SA555) saturate all biotin binding sites from the first step ? > >Yves > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From Melissa.Gonzalez <@t> cellgenesys.com Tue Mar 14 16:29:32 2006 From: Melissa.Gonzalez <@t> cellgenesys.com (Melissa Gonzalez) Date: Tue Mar 14 16:29:42 2006 Subject: [Histonet] RE:H2DCFDA fading,photobleaching and antifade Message-ID: Glenn, I agree with Gayle's comments, and for those of you out there still using anything besides Alexa Fluors, please switch! It will save you so much headache, they are superior to anything I've tried thus far. The Molecular Probes website and handbook are excellent. I still use Vectashield Hardset most of the time however, just because I have not personally observed an improvement over using ProLong Gold mounting media. I also have not seen any amplification in signal by using Streptavidin conjugates of Alexa's, so I usually don't bother with those. But everyone's situation is different, so I never rule anything out. The next thing on my list to try are Qdots, but I've heard mixed reviews. Good luck! Melissa Melissa A. Gonzalez R&D Histology Cell Genesys South San Francisco, CA ph(650) 266-3168 fx(650) 266-3080 Glenn, You wrote: I am using H2DCFDA for the detection of cellular reactive oxygen species following treatment with various agents. Cells are subsequently mounted in anti-fadent with DAPI (Slow Fade Gold with DAPI). The green fluorescence appears awfully weak. I have noticed this in past experiments looking at cytochrome c release with FITC detection. Could the antifadent be the culprit? Don't some antifadent products quench fluorescein right-off-the-bat? Would it be best to just do a standard aqueous mount and be extra careful with light exposure? Reply: What you are experiencing is NOT quenching but photobleaching. FITC is far easier to photobleach when exposed to UV excitation and not performing the staining in the dark (under cover!) The mounting media helps prevent photobleaching but not 100%, however if you use Alexa 488 from Molecular Probes instead of FITC as the fluorophore, you will probably have far better retention of fluorescnce with the Alexa's. It definitely will be brighter and you can look at it longer with UV light. It is not only mounting media but also the choice of fluorophore. There are several mounting medias that Molecular Probes did a comparison on and showed(results available online)which mounting medias worked better for fluoresecence. You can learn a great deal about fluorsceinated fluorophores (FITC, TRITC, Texas Red) versus Alexa dyes that are sulfonated with the latter be superior. These are more more resistant to photobleaching and definitely worth a try. Molecular Probes Handbook explains this, although it is a rather "physical chemistry" oriented subject. The original publication in J HIstochem Cytochem by the Hauglands who developed the use of Alexa dyes is a good read. Microscopy Today just had a wonderful comparison of mounting medias for IFA by Collins, go back in Histonet Archives to access the website for this free article. I have never had a mounting media fade FITC immediately, but I do enjoy good fluroescent signal retention with this after using Molecular Probes hard set Prolong Gold ready to use with or without DAPI. I have use AquaMount, but examination of slide is same day - photobleaching generally occurs more at time of UV light excitation so we take photos at time of viewing sections. There are some ways to increase the signal - we use Strepavidin-Alex dyes with biotinylated secondary or a biotinylated primary to amplify more - Hope this helps. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From AnthonyH <@t> chw.edu.au Tue Mar 14 17:11:44 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Mar 14 17:11:58 2006 Subject: [Histonet] Renal biopsies Message-ID: If the sample is to arrive within 4-5 hours of collection, I would recommend placing the biopsies in Hanks culture media and transport at 4oC. The samples will keep quite well. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward Sent: Wednesday, 15 March 2006 7:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Renal biopsies I want to get a feel for how others receiving renal biopsies handle specimens from outside their institution. Currently outside clients divide the biopsy and submit it to us in 10% NBF, Zeus transport media for IMF and 2.5% gluteraldehyde for EM. One of our outside physicians wants to perform the biopsy at a neighboring hospital (located about a mile from us), have it packaged for transport to us by their pathology department and sent to us on moistened gauze for us to assess and divide appropriately. (This arises from some situations where the material was not divided optimally for the various procedures.) I have reservations about this but I would like to hear how other institutions handle this. Thanks in advance for your help with this. You can respond to me directly or to the whole group. Martha Ward, MT (ASCP) QIHC Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From ja.mitchell <@t> hosp.wisc.edu Tue Mar 14 17:20:41 2006 From: ja.mitchell <@t> hosp.wisc.edu (Mitchell Jean A.) Date: Tue Mar 14 17:20:46 2006 Subject: [Histonet] Tri-State Spring Symposium Message-ID: <583D3E9A1E843445BD54E461D1A2F6F3192C1947@uwhis-xchng2.hosp.wisc.edu> 2006 Tri-State Spring Symposium "Are You Ready For Some Histo?" Hosted by: histology societies of Iowa, Minnesota & Wisconsin Dates: April 19-21, 2006 Meeting Location: Madison Concourse Hotel, Madison, Wisconsin Two-Day Registration - $100.00 Includes: pizza party, vendor exhibits, breaks, lunches, banquet, seminars, choice of afternoon workshops. Workshop topics: collection and management techniques of small tissue specimens, troubleshooting IHC, applying lean principles to the histology laboratory, specimen labeling and tracking, implementing microwave technology, eye structures and techniques of gross examination, cost accounting for the histology laboratory, vibrating blade microtomy. Seminar topics: breast biopsies, introduction to electron microscopy techniques, histology of the exceptional and edible, real forensics in a CSI world Come join us in Madison!! For additional information please contact one of the states representatives; Iowa - Judi Stasko (jstasko@nadc.wsda.gov) Minnesota - Lois Rowe (rowe.lois@mayo.edu) Wisconsin - Jean Mitchell (ja.mitchell@hosp.wisc.edu) From Erin.Wrona <@t> kp.org Tue Mar 14 17:41:16 2006 From: Erin.Wrona <@t> kp.org (Erin.Wrona@kp.org) Date: Tue Mar 14 17:41:42 2006 Subject: [Histonet] Control block and slide management Message-ID: Hello everyone, I am trying to come up with a system for monitoring our control block and slide inventory. How are you all doing it? We have controls for about 120 antibodies, and we often find out that we are out of slides just as we need a control, or find the control block is exhausted just as we need to cut it. My lab director thinks that one of you wonderful people probably has a great system already in place and that we could learn from your experience! Thanks in advance, Erin Wrona Kaiser Permanente San Francisco CONFIDENTIAL OR PRIVILEGED: This communication contains information intended only for the use of the individuals to whom it is addressed and may contain information that is privileged, confidential or exempt from other disclosure under applicable law. If you are not the intended recipient, you are notified that any disclosure, printing, copying, distribution or use of the contents is prohibited. If you have received this in error, please notify the sender immediately by telephone or by returning it by reply email and then permanently deleting the communication from your system. Thank you. From Erin.Wrona <@t> kp.org Tue Mar 14 17:42:07 2006 From: Erin.Wrona <@t> kp.org (Erin.Wrona@kp.org) Date: Tue Mar 14 17:42:25 2006 Subject: [Histonet] Control block and slide management Message-ID: Hello everyone, I am trying to come up with a system for monitoring our control block and slide inventory. How are you all doing it? We have controls for about 120 antibodies, and we often find out that we are out of slides just as we need a control, or find the control block is exhausted just as we need to cut it. My lab director thinks that one of you wonderful people probably has a great system already in place and that we could learn from your experience! Thanks in advance, Erin Wrona Kaiser Permanente San Francisco CONFIDENTIAL OR PRIVILEGED: This communication contains information intended only for the use of the individuals to whom it is addressed and may contain information that is privileged, confidential or exempt from other disclosure under applicable law. If you are not the intended recipient, you are notified that any disclosure, printing, copying, distribution or use of the contents is prohibited. If you have received this in error, please notify the sender immediately by telephone or by returning it by reply email and then permanently deleting the communication from your system. Thank you. From ZhiqingX <@t> orthosurg.ucsf.edu Tue Mar 14 18:57:21 2006 From: ZhiqingX <@t> orthosurg.ucsf.edu (Xing, Zhiqing) Date: Tue Mar 14 19:01:00 2006 Subject: [Histonet] Blood smear Message-ID: <319CB5A5FC297A43944F5939EF6A96C301537887@sfgh05.som.ucsf.edu> I am trying to do X-gal staining on mouse blood smears. But the blood film keeps coming off the slide. I believe the smear is thin enough. After making the smear, I dried it overnight at RT or 2-3 hours in vacuum. Then if I fix it with 100% methanol, the slide would be OK, the blood doesn't come off during the staining, but I don't get any X-gal stain. I tried fixation with acetone for 20 min, the blood stayed on the slide when in acetone, but it came off when transfered to staining solution. I also tried fixation with glutaraldehyde or PFA, the blood just comes off even in the fixative solutions. In the begining, I was using Superfrost plus slides. Somebody on the histonet said the blood smear might not like the plus slides. So I changed to the regular slides, but the problem remains. Can anyone give me some suggestions? Thanks. From lpwenk <@t> sbcglobal.net Tue Mar 14 19:26:52 2006 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Tue Mar 14 19:27:52 2006 Subject: [Histonet] No Practical for HT in 2007(or after?) In-Reply-To: <284.6f03cd0.3144424a@aol.com> Message-ID: <000301c647cf$8aa80f70$cb21d445@HPPav2> Hi - Yes, the HT and HTL practical exams are being discontinued starting in 2007. In other words, 2006 is the last year it will be required. (If you're not interested, please hit "delete" at this point, as I'm going to get wordy.) There was information on the ASCP Board of Registry (BOR) web page. However, ASCP recently redid their entire web page, and this information is in queue to be put back up on the new pages. (In other words, they didn't drop it, it just hasn't been put back up.) I'll fill you in on what I know, learned by reading an email sent to all HT/HTL program directors about 2 months ago, and what was talked about at educator's forum at the 2005 NSH meeting in Florida. Also, I talked with Dr. Blair Holladay (head of ASCP BOR) when the email first came out, and with Gerri Piscorski (exam manager) (312-541-4887), after that, to get more information. Histotechs were the only category still required to do a practical. Med techs don't have to prove they can make a blood smear or can do a Gram stain or can streak an agar plate. Cytotechs don't have to prove they can make a FNA smear or do a Pap stain. Phlebotomists don't have to prove they can get blood from someone with collapsed veins, or do a heel stick on a newborn. All other categories are tested by just a written exam. The majority of histotechs pass the practical portion. Of those that don't pass the practical, the majority of these also did not pass the written portion. In other words, very few people pass the written but fail the practical. Most either pass the practical/fail the written, or they fail both parts, or they pass both parts. So the written portion of the HT/HTL exam was a better indicator of who would pass/fail both parts, than the practical exam. The cost of the grading of the practical exams was very high - flying histotechs and pathologists into Chicago from all over the country, putting them up in hotels for the weekend, feeding them. The graders were not being extravagent - pizza for lunch, sharing cabs to the grading center, etc. The number of slides was reduced from 15 to 9, in part to reduce the number of graders needed, thus reducing the cost. (By statistically picking the right combination of tissues and stains, 9 slides were giving the same pass/fail rate as the previous 15 slides.) The fee charged to the HT/HTL candidates did not cover the cost of grading the practical exam. Not even when an additional $75 fee was added. So ASCP BOR was losing money with each practical exam. Due to concerns about HIPAA, confidentiality, shipping, etc., many candidates were having difficult times obtaining tissue (especially students in college based histotech programs, that were doing rotations in hospital labs for, say, 2 months). This idea of dropping the practical exam has been around for quite a few years. Reducing the number of slides on the practical from 15 to 9 was a compromise, a stepping stone if you would. The people on the ASCP BOR Histotechnology Exam Committee have been taking pictures of poor staining and sectioning artifacts. These will be incorporated into future written exams as troubleshooting and problem-solving questions. What are the causes and how to correct thick/thin sections, wrinkles, folds, splits, microchatter, etc. So the exam is being re-written to incorporate more staining and sectioning problems. Remember, the ASCP BOR Histology Exam Committee with histotechs very involved with NSH, were involved in this discussion/decision. NSH Board has a representative on the Histology Exam Committee/ASCP Board of Governors, who was involved with this topic. ASCP BOR also talked with program directors of HT/HTL schools about this at the NSH convention, asking their opinion. And this topic has been discussed at various committees that I've been on, for the past several years. So it's been coming, slowly, for somewhere over the last 6 years, maybe closer to 10 years, that I'm aware of. Now, some questions you may be having - What is going to happen to the people who didn't pass the practical last year or the year before (2005, 2004)? Each one has been sent a letter, informing them at they must take and pass the practical in 2006 (this year). If they don't pass the practical this year, then they will not have passed their HT or HTL exam. - What about the people taking the HT/HTL exam for the first time this year (2006), who don't pass? They will be given one more year (2007) to take the "make up" practical, in order to pass their HT/HTL exam. So, for just these few people, they will be allowed to take the practical in 2007. But no one else. - How will I know if someone I'm thinking about hiring can really cut or stain, if they don't have to do a practical exam for ASCP? My suggestion - having them do a "practical exam" as part of their interview with you. Hand them 6 blocks, put them in front of a microtome, and give them 20 minutes to section. Then interview them more (to use up time during the drying), and have them load the H&E stainer. While the slides are staining, have a folder of slides for them to look at - some H&E, some special stains. Ask them to identify the stains or the tissue or what's wrong with a poorly stained slide. As long as you use the same type of tissues in the blocks, and the same stained slides, and have EVERY candidate do the test - this is perfectly acceptable way to assess someone you are interviewing for a position. By this time, the H&Es are done. Have them coverslip them. If you still have questions, PLEASE contact Gerry (see direct phone number above). She is very willing to talk with people about this (or any other question you have about the HT/HTL exams. ASCP is THE best place to go to for questions about ASCP. Peggy A. Wenk, HTL(ASCP)SLS Program Director, HT/HTL Programs William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bartholomew2001@aol.com Sent: Saturday, March 11, 2006 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] No Practical for HT in 2007(or after?) Good Day Everyone! I was wondering if anyone can confirm that the HT practical will be discontinued in 2007 (and thereafter)? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Tue Mar 14 19:37:33 2006 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Tue Mar 14 19:38:35 2006 Subject: [Histonet] Education requirements In-Reply-To: <20060314174645.53997.qmail@web33401.mail.mud.yahoo.com> Message-ID: <000901c647d1$086a50c0$cb21d445@HPPav2> It's called the CMP = certification maintenance program. http://www.ascp.org/Certification/AssessmentProducts/CMP/?sm=BOR1 For those who took and passed their ASCP certification after Jan. 1, 2004, their certification is good for only 3 years. After the 3 years, if they want to retain their certification, they either have to take the exam all over again, or they have to provide documentation of a minimum of 36 hours of continuing education (CE) during the intervening 3 years. And they have to keep doing this every three year. For those who took and passed their ASCP certification before Dec. 31, 2003, they are "grandfathered" in. They are certified for life, and do not have to provide any proof of CE. If they want to, they can voluntarily participate in the CMP, and provide the 36 hours of CE. Go to the above web site, and find out exactly how many hours of CE are needed in which topics, and what type of CE can count as how many hours. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy DuBois Sent: Tuesday, March 14, 2006 12:47 PM To: Histonet Subject: [Histonet] Education requirements I was curious whatever happened with the proposed continuing education requirements to maintain ASCP HT certification? Was it implemented and if so what are the terms? We are having a harder time getting our lab director to pay for such things as the NSH teleconferences and the society meetings. But if this was a requirement to maintain licensure, I am sure it would not be an issue. Cindy DuBois, HT ASCP Integrated Pathology --------------------------------- Yahoo! Mail Use Photomail to share photos without annoying attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abright <@t> brightinstruments.com Wed Mar 15 04:04:47 2006 From: abright <@t> brightinstruments.com (Alan Bright) Date: Wed Mar 15 03:55:31 2006 Subject: [Histonet] Mohs frozen Sections Message-ID: I am in the final stages of unique development to aid Mohs frozen sectioning. It would be of great assistance from those of you that carry out this study, if you could inform me if you have Liquid Nitrogen available in your laboratory? Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com From Jacqueline.Malam <@t> rli.mbht.nhs.uk Wed Mar 15 04:44:24 2006 From: Jacqueline.Malam <@t> rli.mbht.nhs.uk (Malam Jacqueline) Date: Wed Mar 15 04:49:32 2006 Subject: [Histonet] Control blocks and slides Message-ID: How about - whoever takes the last one out of the stock box replaces with fresh supply - or everyone monitors stock levels - or give the responsibility of stock level monitoring to someone - or monthly audit Jacqui Malam Lancaster UK DISCLAIMER: This e-mail is confidential and privileged. If you are not the intended recipient please accept our apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform postmaster@rli.mbht.nhs.uk that this message has gone astray before deleting it. Comments or opinions expressed in this email are those of their respective contributors only. The views expressed do not represent the views of the Trust, its management or employees. Morecambe Bay Hospitals NHS Trust is not responsible and disclaims any and all liability for the content of comments written within.Thank you for your co-operation. From O.M.Gallagher <@t> sheffield.ac.uk Wed Mar 15 07:46:54 2006 From: O.M.Gallagher <@t> sheffield.ac.uk (Orla Gallagher) Date: Wed Mar 15 07:47:08 2006 Subject: [Histonet] Fluorescent labelled TRAP staining Message-ID: <44181ACD.26192.E4A7F3@localhost> Dear Histonetters, Thanks for your helpful replies to my query about fluorescence- friendly aqueous mounting media. Our research group routinely use histochemical TRAP staining to identify osteoclasts in decalcified paraffin-embedded bone (using naphthol AS-BI phosphate and pararosaniline). We've also been labelling LR White resin-embedded bones with tetracycyline and calcein and wondered if we could co-localise these fluorescent labels with a fluorescent marker for osteoclasts within the same tissue section? We've found a paper which uses ELF97 phosphatase substrate from Molecular Probes, but would like to know if anyone has tried this on resin-embedded tissues. Thanks, Orla ****************************** Ms.Orla Gallagher Academic Unit of Bone Biology Division of Clinical Sciences (South) D Floor (DU20) Medical School Henry Wellcome Laboratories for Medical Research University of Sheffield Beech Hill Road Sheffield S10 2RX www.sheffield.ac.uk E-mail:o.m.gallagher@sheffield.ac.uk Tel: 0114 271 3783(lab) 0114 271 3954(office) Fax: 0114 271 1711 From SARAH.REEVES <@t> ekht.nhs.uk Wed Mar 15 07:58:00 2006 From: SARAH.REEVES <@t> ekht.nhs.uk (SARAH REEVES) Date: Wed Mar 15 07:58:58 2006 Subject: [Histonet] alcian blue deposit Message-ID: Does anyone suffer with alcian blue deposit? How do you remedy this? ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of East Kent Hospitals NHS Trust. If you are not the intended recipient, be advised that you have received this email in error and that any use, dissemination, forwarding, printing or copying of this email is strictly prohibited. If you have received this email in error please notify the system manager at the following email address: root.postmaster@ekht.nhs.uk www.kentandmedway.nhs.uk This footnote also confirms that although this email message has been swept by MIMEsweeper for the presence of computer viruses, it is strongly recommended that you carry out your own virus scan of this message and any attachments. www.mimesweeper.com ********************************************************************** From Bartholomew2001 <@t> aol.com Wed Mar 15 08:31:40 2006 From: Bartholomew2001 <@t> aol.com (Bartholomew2001@aol.com) Date: Wed Mar 15 08:31:50 2006 Subject: [Histonet] Hmmmm(Certification Changes) Message-ID: <25c.8a778c3.31497f4c@aol.com> Should one suppose that information pertaining to big changes in the Certification process should be a little higher on the priority list? It is lovely that the ASCP decided to redo their entire web site; I am just delighted that the online store works. A little thing like ending the practical for HT/HTL..Bahhhh! Let's put that on the back burner..in six point type! A little searching on the HISTONET archives reveals very LITTLE discussion (read: NONE) about these changes. I do see, however, a lot of posts championing the BOR and its process. Now, surely, this would be the place to put us all in the loop for a change that has been developing for years (if I might paraphrase). Any emails or discussions about this never made it to this public forum. Or, do I mean Republic forum... Any way you slice it, to arbitrarily void someone's passing score on a computer exam- with a late letter- is not only unfair, but a breach of contract. I wonder if this will bring out the lambs or lions in some. From dmccaig <@t> ckha.on.ca Wed Mar 15 10:33:10 2006 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Wed Mar 15 10:35:57 2006 Subject: [Histonet] T Cell Heat retrieval using microwave pressure cooker Message-ID: Hi I am trying to demonstrate T cells using Polyclonal Rabbit Anti human CD3, T cell (DAKO ENVISION). The control tissue is tonsil and using a manual procedure. I have increased the time in the microwave pressure cooker from 5 up to 30 minutes (pH 6 buffer) and have no success. The antibody is incubated for 30 minutes (recommended) but I have gone as high as 1 hour. I am using AEC and Mayers counterstain. Any help or advise would be greatly appreciated. Diana From rjbuesa <@t> yahoo.com Wed Mar 15 10:42:53 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 15 10:42:58 2006 Subject: [Histonet] Control block and slide management In-Reply-To: Message-ID: <20060315164253.31073.qmail@web61225.mail.yahoo.com> Erin: This is how I used to do it: 1- have as few block controls as possible, meaning try to use tissues that are a good control for as many antibodies as possible; 2- on Mondays (usually a slow day in our lab since we used to do late Friday's and Saturday's cases on Sunday) the boxes with the control slides were checked and new were cut if needed. To determine which blocks/controls to cut, we kept a record of tests perform daily that was "totaled" on Fridays. This system worked good for us, BUT BEWARE, never have control slides for more than 2 weeks of being cut sitting in the box; you may find that their "reactivity" may have beed substantially reduced. I found that the controls more easy to "fade" or present reduced reactivity were: Actin; all BCLs; many CDs (3;4;5;10;15;23;25;30;43;56;57;79a; CK20 & CK 34BE12; CMV; E-Cadhedrin; HBcAG; HBsAg; HPV; HSV I&II; Lambda; Myosin; NSE; P53; PSA and TTF-1 If you want I can send you a paper of mine with those findings. Hope this will help you! Ren? J. Erin.Wrona@kp.org wrote: Hello everyone, I am trying to come up with a system for monitoring our control block and slide inventory. How are you all doing it? We have controls for about 120 antibodies, and we often find out that we are out of slides just as we need a control, or find the control block is exhausted just as we need to cut it. My lab director thinks that one of you wonderful people probably has a great system already in place and that we could learn from your experience! Thanks in advance, Erin Wrona Kaiser Permanente San Francisco CONFIDENTIAL OR PRIVILEGED: This communication contains information intended only for the use of the individuals to whom it is addressed and may contain information that is privileged, confidential or exempt from other disclosure under applicable law. If you are not the intended recipient, you are notified that any disclosure, printing, copying, distribution or use of the contents is prohibited. If you have received this in error, please notify the sender immediately by telephone or by returning it by reply email and then permanently deleting the communication from your system. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Relax. Yahoo! Mail virus scanning helps detect nasty viruses! From Bauer.Karen <@t> mayo.edu Wed Mar 15 11:02:16 2006 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen) Date: Wed Mar 15 11:02:43 2006 Subject: [Histonet] Mayer's Hematoxylin Message-ID: Hello to all, I'm looking for a Pre-Made Mayer's Hematoxylin that we can use for our routine H&E staining procedure. We make up our Mayer's Hematoxylin from scratch, but have recently been given permission to buy something that is pre-made. We've tried Harris, but the doctors do not like the differentiation that take place with using that kind of hematoxylin. (We stain by hand, so one tech's dip might be longer or shorter than another tech's dip in the differentiator.) They are seeing variations in nuclear staining, no matter how careful we are with our "dips". I'm looking for something that stains tissues in 5-10 minutes and does not need differentiation. Everything that I've tried so far is not working out to their satisfaction. We use a Gill's III for our FS set up, and they do not want that either (for the routine H&E's). Does anyone have any ideas or tips for me? I'll be greatly appreciative. Thanks in advance, Karen Bauer HT(ASCP) Histology Supervisor Luther Hospital Eau Claire, WI ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. From Dmborel <@t> aol.com Wed Mar 15 11:22:43 2006 From: Dmborel <@t> aol.com (Dmborel@aol.com) Date: Wed Mar 15 11:22:49 2006 Subject: [Histonet] (no subject) Message-ID: <315.27b056.3149a763@aol.com> test From Dmborel <@t> aol.com Wed Mar 15 11:27:09 2006 From: Dmborel <@t> aol.com (Dmborel@aol.com) Date: Wed Mar 15 11:27:20 2006 Subject: [Histonet] Bid Contract Position in Topeka, Kansas Message-ID: <2d0.51adcaa.3149a86d@aol.com> We are in the process of bidding on a large volume contract. If successful, we will need to staff a shift from 1:00 PM to 9:30 PM Monday to Friday for individuals to perform gross tissue exams of skin biopsies and to perform routine histology. This could be filled by a single full time person or a shared position with two or more individuals. Interested parties please contact: David M. Borel, M.D., Medical Director Telephone (785) 272-8246 Website _www.dermpathdx.com_ (http://www.dermpathdx.com/) Email dmborel@aol.com From liz <@t> premierlab.com Wed Mar 15 11:34:12 2006 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Mar 15 11:34:28 2006 Subject: [Histonet] T Cell Heat retrieval using microwave pressure cooker In-Reply-To: Message-ID: <000001c64856$ac776150$c6d48a80@Chlipala> Diana We used dako's ph9 retreival in the pascel with good results and envision rabbit as a detection system. We were staining human thymus grafts in mouse tissue. 30 minute incubation at 1:100. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Wednesday, March 15, 2006 9:33 AM To: Histonet (E-mail) Subject: [Histonet] T Cell Heat retrieval using microwave pressure cooker Hi I am trying to demonstrate T cells using Polyclonal Rabbit Anti human CD3, T cell (DAKO ENVISION). The control tissue is tonsil and using a manual procedure. I have increased the time in the microwave pressure cooker from 5 up to 30 minutes (pH 6 buffer) and have no success. The antibody is incubated for 30 minutes (recommended) but I have gone as high as 1 hour. I am using AEC and Mayers counterstain. Any help or advise would be greatly appreciated. Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1.1444 (20060315) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From PMonfils <@t> Lifespan.org Wed Mar 15 11:46:17 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Mar 15 11:46:25 2006 Subject: [Histonet] Mayer's Hematoxylin Message-ID: <09C945920A6B654199F7A58A1D7D1FDE01717698@lsexch.lsmaster.lifespan.org> Mayer's Hematoxylin can be purchased from Rowley Biochemical Institute, as can a wide range of dyes (both dry powders and readymade solutions), which in fact is the only thing they sell. I have used their products for years and have rarely run into any problems. When I did have one problem with a lot of orcein, they promptly dealt with it, sending me samples of other lots at no charge until I found one that worked to my satisfaction. http://www.rowleybio.com/ > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > Bauer, Karen > Sent: Wednesday, March 15, 2006 9:02 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Mayer's Hematoxylin > > Hello to all, > > I'm looking for a Pre-Made Mayer's Hematoxylin that we can use for our > routine H&E staining procedure. We make up our Mayer's Hematoxylin from > scratch, but have recently been given permission to buy something that is > pre-made. We've tried Harris, but the doctors do not like the > differentiation that take place with using that kind of hematoxylin. (We > stain by hand, so one tech's dip might be longer or shorter than another > tech's dip in the differentiator.) They are seeing variations in nuclear > staining, no matter how careful we are with our "dips". > > I'm looking for something that stains tissues in 5-10 minutes and does not > need differentiation. Everything that I've tried so far is not working > out to their satisfaction. We use a Gill's III for our FS set up, and > they do not want that either (for the routine H&E's). > > Does anyone have any ideas or tips for me? I'll be greatly appreciative. > > Thanks in advance, > > Karen Bauer HT(ASCP) > Histology Supervisor > Luther Hospital > Eau Claire, WI > ********************Confidentiality Notice******************** > > > > This message is intended for the sole use of the individual and entity to > whom it is addressed, and may contain information that is privileged, > confidential and exempt from disclosure under applicable law. Any > unauthorized review, use, disclosure or distribution of this email > message, including any attachment, is prohibited. If you are not the > intended recipient, please advise the sender by reply email and destroy > all copies of the original message. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From mauger <@t> email.chop.edu Wed Mar 15 12:04:59 2006 From: mauger <@t> email.chop.edu (Joanne Mauger) Date: Wed Mar 15 12:05:43 2006 Subject: [Histonet] T Cell Heat retrieval using microwave pressure cooker Message-ID: Diana, Try using EDTA buffer (pH 8) for retrieval. It works for us! Jo >>> "Diana McCaig" 03/15/06 11:33 AM >>> Hi I am trying to demonstrate T cells using Polyclonal Rabbit Anti human CD3, T cell (DAKO ENVISION). The control tissue is tonsil and using a manual procedure. I have increased the time in the microwave pressure cooker from 5 up to 30 minutes (pH 6 buffer) and have no success. The antibody is incubated for 30 minutes (recommended) but I have gone as high as 1 hour. I am using AEC and Mayers counterstain. Any help or advise would be greatly appreciated. Diana _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hej01 <@t> health.state.ny.us Wed Mar 15 12:06:55 2006 From: hej01 <@t> health.state.ny.us (Helen E Johnson) Date: Wed Mar 15 12:07:12 2006 Subject: [Histonet] Bouin Solution Message-ID: Hi Histonetters, Can someone please tell me what is the standard Bouin fixation time for mouse small intestine. Thanks. Helen (hej01@health.state.ny.us) From ploykasek <@t> phenopath.com Wed Mar 15 12:20:58 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Wed Mar 15 12:21:18 2006 Subject: [Histonet] T Cell Heat retrieval using microwave pressure cooker In-Reply-To: Message-ID: Just an fyi for a process that we use whenever working up a new antibody. We read the data sheet, read any applicable literature. We usually "bracket" the recommended titer, ex- if data sheet recommends 1:50 we would do 1:25, 1:50, 1:100. We try 3-4 different pretreatments with these 3 titers. If data sheet or literature recommend a certain pretreatment we try that + at least 2 other different pretreatment. For example, if a citrate heat pretreatment is recommended, we would try citrate & heat, EDTA or TRIS & heat, and an enzyme. After this initial round, we usually have some idea of which pretreatment will work, and a better idea of titer. Usually have to do 1 more round using best pretreatment with different titers or maybe add a different control. Then we select titer & pretreatment, and start the validation (a whole 'nother story). Hope this helps. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > > Hi > I am trying to demonstrate T cells using Polyclonal Rabbit Anti human CD3, T > cell (DAKO ENVISION). The control tissue is tonsil and using a manual > procedure. I have increased the time in the microwave pressure cooker from 5 > up to 30 minutes (pH 6 buffer) and have no success. The antibody is incubated > for 30 minutes (recommended) but I have gone as high as 1 hour. I am using > AEC and Mayers counterstain. Any help or advise would be greatly appreciated. > Diana > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From sjchtascp <@t> yahoo.com Wed Mar 15 12:26:00 2006 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Wed Mar 15 12:26:04 2006 Subject: [Histonet] Brdu IHC on histogel Message-ID: <20060315182600.96052.qmail@web38215.mail.mud.yahoo.com> Has anyone out there done Brdu IHC staining on cultured cells in a histogel cell block FFPE. We are having no luck staining cultured cell for Brdu in well plates Steve --------------------------------- Yahoo! Mail Use Photomail to share photos without annoying attachments. From JKOBLER <@t> PARTNERS.ORG Wed Mar 15 12:55:49 2006 From: JKOBLER <@t> PARTNERS.ORG (Kobler, James) Date: Wed Mar 15 12:55:53 2006 Subject: [Histonet] question Message-ID: I'm soliciting advice about the best ways to examine fat tissue histologically. We are preparing fat tissue for autografting (reinjection through a needle) and want to look at damage to the fat tissue caused by extrusion through a needle. Subcutaneous fat is surgically excised, scraped to remove most of the fibrous component, stuffed into a syringe and then extruded out through a fine needle under considerable pressure. I would appreciate any suggestions about the best ways to compare normal fat tissue with the extruded fat 'goop'. In particular we would like to be able to quantify the number of viable cells. Thanks, Jim Kobler, Ph.D., Dept. of Surgery, Harvard Medical School From gcallis <@t> montana.edu Wed Mar 15 13:34:36 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Mar 15 13:34:56 2006 Subject: Choices for Re: [Histonet] Mayer's Hematoxylin In-Reply-To: References: Message-ID: <6.0.0.22.1.20060315122420.01b094f8@gemini.msu.montana.edu> Karen, There are many options - Richard Allan Mayers, Poly Scientific, Newcomers Supply, Thermo may have it too. Gill II or other progressive hematoxylins (Richard Allan Hematoxylin 1 and their classic 7211 (Hope I have that correct?) all work well too, staining in 1 1/2 min or so, no differentiation is really needed with progressive hematoxylins, just bluing and the appropriate water rinses. Have Richard Allan send you some samples to try, and see which one you like the best. They have an excellent staining manual or troubleshooting guide on their website. This guide will tell you how to have optimal staining results (let your pathologists see your results) with timing suggestions, etc. And that is where you would have to make adjustments - time in stain will be important. Our students and other techs never have to worry about the dipping factor, it is pretty much "recipe" using a timer and we have no staining problems. Gill III is the strongest of the Gills, you should try Gill 1, and Gill II - we used Gill II on tissue sections for years - Thermo has this also. Gill I is half as strong as Gill II which is half as strong as Gill III, so the concentration of the dye may mean less staining time in hematoxylin. Good luck At 10:02 AM 3/15/2006, you wrote: >Hello to all, > >I'm looking for a Pre-Made Mayer's Hematoxylin that we can use for our >routine H&E staining procedure. We make up our Mayer's Hematoxylin from >scratch, but have recently been given permission to buy something that is >pre-made. We've tried Harris, but the doctors do not like the >differentiation that take place with using that kind of hematoxylin. (We >stain by hand, so one tech's dip might be longer or shorter than another >tech's dip in the differentiator.) They are seeing variations in nuclear >staining, no matter how careful we are with our "dips". > >I'm looking for something that stains tissues in 5-10 minutes and does not >need differentiation. Everything that I've tried so far is not working >out to their satisfaction. We use a Gill's III for our FS set up, and >they do not want that either (for the routine H&E's). > >Does anyone have any ideas or tips for me? I'll be greatly appreciative. > >Thanks in advance, > >Karen Bauer HT(ASCP) >Histology Supervisor >Luther Hospital >Eau Claire, WI >********************Confidentiality Notice******************** > > > >This message is intended for the sole use of the individual and entity to >whom it is addressed, and may contain information that is privileged, >confidential and exempt from disclosure under applicable law. Any >unauthorized review, use, disclosure or distribution of this email >message, including any attachment, is prohibited. If you are not the >intended recipient, please advise the sender by reply email and destroy >all copies of the original message. Thank you. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From ploykasek <@t> phenopath.com Wed Mar 15 13:35:34 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Wed Mar 15 13:35:59 2006 Subject: [Histonet] Mayer's Hematoxylin In-Reply-To: Message-ID: Karen, our research department uses Mayer's hematolxylin from BBC. They use it as an IHC counter stain, and it works well. We have not used it for H&E. The phone # for BBC is 360-629-4477. Patti Loykasek Hello to all, > > I'm looking for a Pre-Made Mayer's Hematoxylin that we can use for our routine > H&E staining procedure. We make up our Mayer's Hematoxylin from scratch, but > have recently been given permission to buy something that is pre-made. We've > tried Harris, but the doctors do not like the differentiation that take place > with using that kind of hematoxylin. (We stain by hand, so one tech's dip > might be longer or shorter than another tech's dip in the differentiator.) > They are seeing variations in nuclear staining, no matter how careful we are > with our "dips". > > I'm looking for something that stains tissues in 5-10 minutes and does not > need differentiation. Everything that I've tried so far is not working out to > their satisfaction. We use a Gill's III for our FS set up, and they do not > want that either (for the routine H&E's). > > Does anyone have any ideas or tips for me? I'll be greatly appreciative. > > Thanks in advance, > > Karen Bauer HT(ASCP) > Histology Supervisor > Luther Hospital > Eau Claire, WI > ********************Confidentiality Notice******************** > > > > This message is intended for the sole use of the individual and entity to whom > it is addressed, and may contain information that is privileged, confidential > and exempt from disclosure under applicable law. Any unauthorized review, use, > disclosure or distribution of this email message, including any attachment, is > prohibited. If you are not the intended recipient, please advise the sender by > reply email and destroy all copies of the original message. Thank you. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From gcallis <@t> montana.edu Wed Mar 15 13:39:07 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Mar 15 13:39:19 2006 Subject: Mouse intestine in [Histonet] Bouin Solution In-Reply-To: References: Message-ID: <6.0.0.22.1.20060315123643.01b56cc8@gemini.msu.montana.edu> We would fix it no more than overnight although 72 hours is permissable, transfer to 70% and do a couple of changes in this alcohol to help remove picric acid. Hopefully you are rinsing the fecal contents out before doing this, we would actually then fill the intestine with Bouins using a syringe. Messy but really fixes villi nicely. At 11:06 AM 3/15/2006, you wrote: >Hi Histonetters, > Can someone please tell me what is the standard Bouin fixation time >for mouse small intestine. >Thanks. Helen (hej01@health.state.ny.us) > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 From runyanc <@t> mail.nih.gov Wed Mar 15 14:03:56 2006 From: runyanc <@t> mail.nih.gov (Runyan, Caroline (NIH/NIMH) [F]) Date: Wed Mar 15 14:04:03 2006 Subject: [Histonet] c-fos immuno in nonhuman primate Message-ID: Hello, Has anyone stained primate tissue for c-fos? If so, what antibody was used and how was the signal:noise? I've been trying to pick one out to try but none look like they would be too great. Thanks, Caroline From john.mcginley <@t> colostate.edu Wed Mar 15 14:12:42 2006 From: john.mcginley <@t> colostate.edu (John McGinley) Date: Wed Mar 15 14:12:53 2006 Subject: [Histonet] IHCRG listserv Message-ID: <003301c6486c$d0f47f70$23255281@CAS.ColoState.EDU> Hi, For those of you who are current members of the NSH Immunohistochemistry Resource Group (IHCRG) please read below. The IHCRG listserv that was hosted on neo.agsci.colostate.edu is no longer active due to an update problem that arose on Monday morning, essentially crippling the mailman software used to manage and distribute email for this listserv. An attempt was made to restore key files from backup copies. However, the software updates which caused the problem changed file locations and dependencies so the backups were useless. While re-installing the software is an option the same problem is very likely to rear it's ugly head again in the near future. Rather than installing a fresh copy of mailman and re-creating the listserv I have decided to move it to a commercial provider for reasons delineated below. 1. Like most of you I'm a bench tech and not a systems administrator. 2. I need a listserv that works for me, not the other way around. 3. I need less stress in my life, not more. I have already moved the lists for the Colorado state society to Google Groups, which I tested earlier this week and the results were promising. There are no banner ads in email messages like there are with other free groups like Yahoo. I will be setting up the new list for the IHCRG tonight. However, due to the large number of members in this group it is likely to be flagged for approval before the group can be activated. The approval process takes about 24-48 hrs. Once approved members will receive a message from "noreply@googlegroups.com" with the following subject line: "You have been added to IHCRG". The body will say something like " mcginleyj@gmail.com has added you to the IHCRG group with this message: ...". If Google decides not to approve my request for direct subscriptions you may receive an invitation message to the group rather than a subscription notice. A link should be provided for you to join the group in the event of invitation only requests. In the meantime, please do not send messages to the old address (ihcrg@neo.agsci.colostate.edu). The new address will be available soon and hopefully this will be the last move for the IHCRG listserv (fingers crossed). Thank you for you patience. Regards, John McGinley, IHCRG webmaster and listserv moderator From jkiernan <@t> uwo.ca Wed Mar 15 15:08:51 2006 From: jkiernan <@t> uwo.ca (John A. Kiernan) Date: Wed Mar 15 15:08:57 2006 Subject: [Histonet] Blood smear References: <319CB5A5FC297A43944F5939EF6A96C301537887@sfgh05.som.ucsf.edu> Message-ID: <44188263.A8A6F834@uwo.ca> Dear Xing Zhiqing, You report two problems: 1. Detachment from the slide, and 2. Failure to detect beta-galactosidase activity with the X-gal substrate. Some of your methods are decidedly unconventional! Vacuum drying? 20 mins in acetone? Glutaraldehyde? Are you also using an unconventional X-gal method? The conventional method has been used for many years and is considered very reliable. Have you tried it? Problem 1. Detachment. A blood film made in the usual way stays very solidly on the slide. See any practical microtechnique book, or get a haematology technician to show you how. The film has to be spread in the correct way, and the slides must be clean (meaning no grease or dust). Positively charged slides should be OK, but people were making blood films 100 years ago with ordinary glass slides. Drying takes minutes. Methanol fixation takes seconds. Acetone (why?) also works almost instantaneously. Glutaraldehyde is a fixative for electron microscopy that inhibits enzymes (though some activity can be spared). Denaturation with a liquid such as methanol or acetone also inhibits enzyme activity. Why are you trying all these unusual preparative methods? Problem 2. No blue product. The simplest explanation is that no blood cells contain a beta-galactosidase. Have you done the method with a similarly prepared film of blood (or other cells) known to give a positive reaction for beta-galactosidase? Which enzyme are you after? Are you staining for endogenous beta-galactosidase (a lysosomal enzyme that works at pH~5) or for the bacterial lac-Z enzyme (pH~7+)? The lac-Z gene is often coupled to more interesting genes in transfection experiments etc. Cells that express the interesting gene also make a beta-galactosidase that can generate an insoluble blue pigment from X-gal at pH>7. John Kiernan Anatomy, UWO London, Canada ________________ "Xing, Zhiqing" wrote: > > I am trying to do X-gal staining on mouse blood smears. But the blood > film keeps coming off the slide. I believe the smear is thin enough. > After making the smear, I dried it overnight at RT or 2-3 hours in > vacuum. Then if I fix it with 100% methanol, the slide would be OK, the > blood doesn't come off during the staining, but I don't get any X-gal > stain. I tried fixation with acetone for 20 min, the blood stayed on the > slide when in acetone, but it came off when transfered to staining > solution. I also tried fixation with glutaraldehyde or PFA, the blood > just comes off even in the fixative solutions. In the begining, I was > using Superfrost plus slides. Somebody on the histonet said the blood > smear might not like the plus slides. So I changed to the regular > slides, but the problem remains. > Can anyone give me some suggestions? Thanks. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Wed Mar 15 15:46:04 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Mar 15 15:46:16 2006 Subject: Beat Gal Re: [Histonet] Blood smear In-Reply-To: <44188263.A8A6F834@uwo.ca> References: <319CB5A5FC297A43944F5939EF6A96C301537887@sfgh05.som.ucsf.edu> <44188263.A8A6F834@uwo.ca> Message-ID: <6.0.0.22.1.20060315143401.01b33fa0@gemini.msu.montana.edu> A very reliable Beta Gal Staining kit can be purchased from Roche (cat.no. 1 828 673). For proper staining, there are fixation requirements to optimize staining but a good positive control (we used cells transfected with a B galactosidaase encoding construct) as it is really necessary to know if kit i.e. staining is working correctly. The kit insert is highly informative and probably found on Roch website. >Problem 2. No blue product. >The simplest explanation is that no blood cells >contain a beta-galactosidase. Have you done the >method with a similarly prepared film of blood (or >other cells) known to give a positive reaction for >beta-galactosidase? > >Which enzyme are you after? >Are you staining for endogenous beta-galactosidase >(a lysosomal enzyme that works at pH~5) or for the >bacterial lac-Z enzyme (pH~7+)? The lac-Z gene is >often coupled to more interesting genes in >transfection experiments etc. Cells that express >the interesting gene also make a >beta-galactosidase that can generate an insoluble >blue pigment from X-gal at pH>7. > >John Kiernan >Anatomy, UWO >London, Canada >________________ >"Xing, Zhiqing" wrote: > > > > I am trying to do X-gal staining on mouse blood smears. But the blood > > film keeps coming off the slide. I believe the smear is thin enough. > > After making the smear, I dried it overnight at RT or 2-3 hours in > > vacuum. Then if I fix it with 100% methanol, the slide would be OK, the > > blood doesn't come off during the staining, but I don't get any X-gal > > stain. I tried fixation with acetone for 20 min, the blood stayed on the > > slide when in acetone, but it came off when transfered to staining > > solution. I also tried fixation with glutaraldehyde or PFA, the blood > > just comes off even in the fixative solutions. In the begining, I was > > using Superfrost plus slides. Somebody on the histonet said the blood > > smear might not like the plus slides. So I changed to the regular > > slides, but the problem remains. > > Can anyone give me some suggestions? Thanks. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From JMacDonald <@t> mtsac.edu Wed Mar 15 16:35:30 2006 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Mar 15 16:35:47 2006 Subject: [Histonet] Mayer's Hematoxylin Message-ID: Karen, Our students are using Hematoxylin 1, by Richard staining by hand, so there is great variation of tec students, but very little in the staining of the nuclei.&nb Richard-Allan has a great chart for optimizing the H&E with their r eagents. Jennifer MacDonald -----histonet-bounces@lists.utsouthweste To: From: "Bauer, Sent by: histonet-bounces@lists.uts Date: 03/15/2006 09:02AM Subject: [Histonet] Mayer's Hello to all, I'm looking for a Pre-Made Mayer's H for our routine H&E staining procedure. &nbs Mayer's Hematoxylin from scratch, but have recently been g permission to buy something that is pre-made. We've tried Harris , but the doctors do not like the differentiation that take place with usin tech's dip migh the differentiator.) &nbs staining, no matter how careful we I'm looking for something that stains ti does not need differentiation. Everything t is not working out to their satisfaction. We us for our FS set up, and they do not want that either (for the routine H&E's). Does anyone have any ideas or tips for me? &nbs appreciative. Thanks in advance, Karen Baue Histology Supervisor Luther Hospital Eau Claire, ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and en tity to whom it is addressed, and may contain information that is privilege applicable law. Any unauth distribution of this email message, inclu prohibited. If you are not the intended recipient, the sender by reply email and destroy all copies of the origi message. Thank you. ______________ ______________________ 5F__ Histonet mailing list Histonet@lists.utsouth [1]http://lists.utsouthwestern.edu/mailman/listinfo References 1. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/ From fmonson <@t> wcupa.edu Wed Mar 15 17:07:54 2006 From: fmonson <@t> wcupa.edu (Monson, Frederick ) Date: Wed Mar 15 17:08:05 2006 Subject: [Histonet] Blood smear Message-ID: Dear Zhiging Xing, I have never lost even a part of a blood smear, or a mesentery spread, from a clean slide after proper drying. Although it has long been clear that slides, out of the box, are considered clean, in fact, slides in an opened box can pick up all kinds of 'bad' karma in a functioning laboratory. Thus, throughout my career, I have always kept a scrupulously cleaned jar filled with 70% ethanol into which I have placed slides, out-of-the-box, that I have cleaned with Lava soap (between thumb and (sore) fore finger (left hand!)), then, rinsed in a stream of running hot water, immersed in cold water in a beaker (then run for 5 minutes), and finally 5x rinses with deionized water before being relocated into the 70% ethanol jar, one at a time (with gloves on!!!). Needless to say, such practices have conspired to keep me from earning a living in histotechnology, but I have NEVER lost even a part of a blood smear to such slides after the smear was FULLY air dried!! Even when I have rushed, only the thicker parts of the smear are at risk of 'falling' off. The feathered edges, bless them, always remain intact for quality analysis, and the basophil(e)s never disintegrate unless they are weak. Cheers to you and to all, and please forgive my rip-arte'. Fred (Monson) Frederick C. Monson, PhD Technical Director Light, Electron, X-Ray and Scanning Probe Imaging and Analysis Center (LEXSPIAC) Large Scientific Instrument Core Geology, West Chester University S. Church St. and W. Rosedale Ave. West Chester, PA, 19320 610-738-0437 fmonson@wcupa.edu URL: http://darwin.wcupa.edu/cgi-bin/casireserve.cgi ============================================ Knowledge is the key to happiness. Ignorance might be the key to contentment. ============================================ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Xing, Zhiqing Sent: Tuesday, March 14, 2006 7:57 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blood smear I am trying to do X-gal staining on mouse blood smears. But the blood film keeps coming off the slide. I believe the smear is thin enough. After making the smear, I dried it overnight at RT or 2-3 hours in vacuum. Then if I fix it with 100% methanol, the slide would be OK, the blood doesn't come off during the staining, but I don't get any X-gal stain. I tried fixation with acetone for 20 min, the blood stayed on the slide when in acetone, but it came off when transfered to staining solution. I also tried fixation with glutaraldehyde or PFA, the blood just comes off even in the fixative solutions. In the begining, I was using Superfrost plus slides. Somebody on the histonet said the blood smear might not like the plus slides. So I changed to the regular slides, but the problem remains. Can anyone give me some suggestions? Thanks. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AFoshey <@t> chw.org Wed Mar 15 17:24:55 2006 From: AFoshey <@t> chw.org (Foshey, Annette) Date: Wed Mar 15 17:24:59 2006 Subject: [Histonet] Zinc formalin as a fixative Message-ID: <9E6D52F532809247BDA1783680E92C565868B5@CHWEXC.chwi.chswi.org> Our new medical director is requesting to use Zinc formalin. I have not used this fixative before and I need your help. We have a small volume and place everything on one processor for an overnight run. Please give me your insights. We use VIP embedding medium for paraffin and use graded alcohols (70, 80, 95, and 100) and Xylene on the processor. Thank you in advance for your information, Annette Foshey, HT Team Leader Children's Hospital of Wisconsin ************************** Children's Hospital and Health System recognizes that unencrypted e-mail is insecure and does not guarantee confidentiality. The confidentiality of replies to this message cannot be guaranteed unless the replies are encrypted. From JMacDonald <@t> mtsac.edu Wed Mar 15 21:13:57 2006 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Mar 15 21:14:14 2006 Subject: [Histonet] California Society for Histotechnology Message-ID: The program is now available for the CSH symposium to be held in May. If you are interested in an email version please let me now.&nbs to get you Jennifer MacDonald From peptolab <@t> hamptons.com Wed Mar 15 23:04:29 2006 From: peptolab <@t> hamptons.com (Jeff Silverman) Date: Wed Mar 15 23:04:35 2006 Subject: [Histonet] Macrophages- take your pick or use them all Message-ID: <000501c648b7$1b360d20$6401a8c0@jeffrey028c8d9> Katy, Each of these have their pitfalls, limitations, and functional implications and could form a useful panel to characterize monocyte/macrophage/histiocyte populations and differentiation. F4/80 antigen is an approximately 125 kDa transmembrane protein expressed by a majority of mature macrophages. However, other cell types such as Langerhans cells and liver Kupffer cells have been reported to express this antigen. Expression of F4/80 commences during early myeloid development with approximately 30% of BM cells staining. F4/80 is upregulated on all BM cells stimulated in vitro with M-CSF. It has been shown that some cytokines downregulate the expression of F4/80 resulting in lack of F4/80 antigen on a subpopulation of macrophages, especially in the lymphoid microenvironment in vivo. CD68 is a cytoplasmic antigen, a membrane glycoprotein closely associated with lysosomes. Langerhans' cells and other dendritic cells are commonly CD68 negative. CD68 is expressed throughout monocyte differentiation, usually more intense in macrophages than monocytes. Granulocytic precursors and mast cells may also exhibit CD68 positivity. Factor-XIIIa-positive dendrocytes are resident dermal and stromal cells in collagenous connective tissue that belong to the monocyte-macrophage-dendrocyte lineage. Factor XIIIa is a tissue tranglutaminase that catalyses the cross linkage of fibrin and many other matrix proteins, and it modulates fibroblast cytoskeleton and adhesion. FXIIIa+ denritic histiocytes are numerous in and are considered to play a pivotal role in the skin response to injury, wound healing, and inflammation and form large populations in many so-called fibrohistiocytic tumors. These cells appear to differentiate into CD68+ phagocytic macrophages, especially in xanthogranuloma. They can be rounded monocytoid, epithelioid to histiocytoid or quite dendritic and fibroblast-like. Jeff Silverman HT HTL QIHC (ASCP) Southside Hospital North Shore Long Island Jewish Health System Bay Shore, New York USA From c.m.vanderloos <@t> amc.uva.nl Thu Mar 16 01:53:52 2006 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Thu Mar 16 01:54:02 2006 Subject: [Histonet] RE: T Cell Heat retrieval using microwave pressure Message-ID: <26f8c3026f6a3b.26f6a3b26f8c30@amc.uva.nl> Diana, I am using the rabbit anti-CD3 monoclonal antibody (SP7) from LabVision at 1:500 (60 min, RT) visualized by EnVision anti-rabbit/HRP and DAB+. HIER is done with Tris-EDTA pH9.0 (15 min boiling + 10 min cool-down). In case you prefer citrate pH6.0 then go down to 1:200 with the primary. Good luck! Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands phone: +31 20 5665631 Date: Wed, 15 Mar 2006 11:33:10 -0500 From: "Diana McCaig" Subject: [Histonet] T Cell Heat retrieval using microwave pressure cooker To: "Histonet \(E-mail\)" [1]histonet@lists.utsouthwestern.edu Hi I am trying to demonstrate T cells using Polyclonal Rabbit Anti human CD3, T cell (DAKO ENVISION). The control tissue is tonsil and using a manual procedure. I have increased the time in the microwave pressure cooker from 5 up to 30 minutes (pH 6 buffer) and have no success. The antibody is incubated for 30 minutes (recommended) but I have gone as high as 1 hour. I am using AEC and Mayers counterstain. Any help or advise would be greatly appreciated. Diana References 1. mailto:histonet@lists.utsouthwestern.edu From c.m.vanderloos <@t> amc.uva.nl Thu Mar 16 02:10:08 2006 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Thu Mar 16 02:10:16 2006 Subject: [Histonet] Re: double/triple staining with streptavidin-Alexa Message-ID: <26ff1c326fc394.26fc39426ff1c3@amc.uva.nl> Dear Yves, What Gayle Callis already mentioned about avidin-biotin blocking before the first and the second sequence is indeed the only thing you can do when combining two biotin techniques in double staining. However, in general I don't recommend this for multiple staining. To my opinion there is still a great risk for false positive results. As Gayle described, the multistep procedure combining one unlabeled and one biotinylated antibody works fine. I would like to add the option of biotinylating or the labeling with an Alexa fluorochrome of one of your primaries using the Zenon kit. Good luck! Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands phone: +31 20 5665631 Date: Tue, 14 Mar 2006 14:13:49 -0700 From: Gayle Callis Subject: Re: [Histonet] double/triple staining with streptavidin-Alexa Fluor To: Yves Heremans , Histonet@lists.utsouthwestern.edu Yes, however, using an avidin/biotin blocks before each antibody to ensure 1) quenching endog biotin before first and quenching any residual biotin left over from first antibody sequence before you do the second primary sequence. We do double IFA staining with two rat antimouse monoclonals in the following way with one purified monoclonal and one biotinylated monoclonal primary. Frozen sections fixed with acetone alcohol mixture at RT after overnight drying Normal serum block matched to host of secondary used Strepavidin/biotin block (Vector) Rat antiMouse monoclonal Donkey antiRat F(ab')2 frag of IgG conjugated to RRX Rat serum block, 5% for 15 minutes Pri! mary #2 r From kemlo.rogerson <@t> waht.swest.nhs.uk Thu Mar 16 02:33:20 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Mar 16 02:32:39 2006 Subject: [Histonet] Zinc formalin as a fixative Message-ID: Don't leave tissue in zinc formalin too long it will go very hard. Some recommend washing out the zinc before processing but that always confused me as I assume you wash out (or reverse) the effects of formalin fixation depending therefore more on the zinc. Haematoxylin staining is enhance because I assume zinc acts as a mordant and binds it to nucleic acids; in my experience it can result in a very 'strong' nuclear stain with some 'overstaining' of the cytoplasm. I assume it is used because of its reduced toxity compared to lead, but I assume it is still toxic to some degree; I thought it was implicated in dementia but how would I know I used it too much in the past. Now where is my slide rule? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 "Following the line of least resistance makes both men and rivers crooked" This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Foshey, Annette [mailto:AFoshey@chw.org] Sent: Wednesday, March 15, 2006 11:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Zinc formalin as a fixative Our new medical director is requesting to use Zinc formalin. I have not used this fixative before and I need your help. We have a small volume and place everything on one processor for an overnight run. Please give me your insights. We use VIP embedding medium for paraffin and use graded alcohols (70, 80, 95, and 100) and Xylene on the processor. Thank you in advance for your information, Annette Foshey, HT Team Leader Children's Hospital of Wisconsin ************************** Children's Hospital and Health System recognizes that unencrypted e-mail is insecure and does not guarantee confidentiality. The confidentiality of replies to this message cannot be guaranteed unless the replies are encrypted. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo.rogerson <@t> waht.swest.nhs.uk Thu Mar 16 02:52:09 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Mar 16 02:51:26 2006 Subject: [Histonet] question Message-ID: Lipid Autoradiography may be the answer; label the removed fat with an isotope (tritium is favoured as its beta particle track is about 1.5 u) and then redetect with a nuclear track emulsion. I suppose you stain a 'control' slide with a fat stain (Oil red O or Sudan Black) then superimpose the results of the autoradiography. If you are interested then I can give you the appropriate references. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 "Following the line of least resistance makes both men and rivers crooked" This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Kobler, James [mailto:JKOBLER@PARTNERS.ORG] Sent: Wednesday, March 15, 2006 6:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] question I'm soliciting advice about the best ways to examine fat tissue histologically. We are preparing fat tissue for autografting (reinjection through a needle) and want to look at damage to the fat tissue caused by extrusion through a needle. Subcutaneous fat is surgically excised, scraped to remove most of the fibrous component, stuffed into a syringe and then extruded out through a fine needle under considerable pressure. I would appreciate any suggestions about the best ways to compare normal fat tissue with the extruded fat 'goop'. In particular we would like to be able to quantify the number of viable cells. Thanks, Jim Kobler, Ph.D., Dept. of Surgery, Harvard Medical School _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Eric <@t> ategra.com Wed Mar 15 16:27:37 2006 From: Eric <@t> ategra.com (Eric Dye (ext 223)) Date: Thu Mar 16 07:16:09 2006 Subject: [Histonet] State of Florida: HistologyTechnologists and Supervisors - Great Location- Great Pay!!!!! Message-ID: Fellow-Histonetters - I am presently on a search for one of my best clients in South Florida. They are seeking to hire Florida Lic'd Histo Technologists and Supervisors. The positions offer a great opportunity and great benefits. Must be Licensed in Florida and a Certified (ASCP) Histologist. 1. BENCH HISTOTECHNOLOGIST Day Shift Monday thru Friday No Weekends, No Call Routine Histology-No Grossing Full Benefits 2. 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Eric - 800 466 9919 ext 223 PS: If you are interested any other state, also please call me at 800 466 9919 ext 223 --------------------------------------------------------- Note: this message is intended for: Fellow-Histonetters at histonet@lists.utsouthwestern.edu --------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing 800 466 9919 ext 223 - voice 407 671 6075 - fax eric@ategra.com To Learn More About Ategra: http://www.ategra.com ---------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. ---------------------------------------------------------- From cpomajzl <@t> cpllabs.com Thu Mar 16 07:49:03 2006 From: cpomajzl <@t> cpllabs.com (Chris Pomajzl) Date: Thu Mar 16 07:46:34 2006 Subject: [Histonet] Large Sections: Prostate Message-ID: <002c01c64900$63013910$26fca8c0@CSP> One of our pathologists is interested in getting a whole section of a bisected prostate with subsequent staining. This would require very large glass slides and a special microtome (I would imagine). This is for research purposes. Does anyone have this capability, or do you have information on where it can be done? Thanks in advance. Chris Pomajzl, HTL (ASCP) Histology Supervisor Clinical Pathology Laboratories, Inc. 9200 Wall Street Austin, Texas 78754 512.873.1660 (o) 512.873.5004 (f) cpomajzl@cpllabs.com CONFIDENTIALITY NOTICE: This communication is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If you are not the intended recipient, you are notified that any use, dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this message in error, please notify the sender immediately and destroy this communication. From kemlo.rogerson <@t> waht.swest.nhs.uk Thu Mar 16 08:06:42 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Mar 16 08:06:05 2006 Subject: [Histonet] Large Sections: Prostate Message-ID: Loads of UK labs are using large processing cassettes and then slides to deal with prostates and neck dissection specimens; I don't think you need a large microtome just the large cassette holders; any of the Histology Supply Companies should have what you need. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 "Following the line of least resistance makes both men and rivers crooked" This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Chris Pomajzl [mailto:cpomajzl@cpllabs.com] Sent: Thursday, March 16, 2006 1:49 PM To: HISTONET Subject: [Histonet] Large Sections: Prostate One of our pathologists is interested in getting a whole section of a bisected prostate with subsequent staining. This would require very large glass slides and a special microtome (I would imagine). This is for research purposes. Does anyone have this capability, or do you have information on where it can be done? Thanks in advance. Chris Pomajzl, HTL (ASCP) Histology Supervisor Clinical Pathology Laboratories, Inc. 9200 Wall Street Austin, Texas 78754 512.873.1660 (o) 512.873.5004 (f) cpomajzl@cpllabs.com CONFIDENTIALITY NOTICE: This communication is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If you are not the intended recipient, you are notified that any use, dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this message in error, please notify the sender immediately and destroy this communication. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> Mercer.edu Thu Mar 16 09:19:46 2006 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Thu Mar 16 09:20:17 2006 Subject: [Histonet] Large Sections: Prostate In-Reply-To: <002c01c64900$63013910$26fca8c0@CSP> Message-ID: <01M04AVO2IRY8WZLNQ@Macon2.Mercer.edu> Hi Chris, Actually you can do this yourself, I have mounted the prostate gland on a 2 by 2 slide if not too large. Make sure the knife clearance is adequate for larger blocks on your microtome or it will not work. You will have to have a sliding microtome for the job. The embedded block can be mounted on a block of wood the size of the cassette to fit into your chuck. The tissue is processed pretty much like your regular specimens if cut thin enough. The specimen can be wrapped in lens paper used to clean scopes by stapling the edges. After processing, use a slide box to embed in paraffin and cut off the excess after solidified. Mount it on your wooden block by heating the back of the paraffin block and submerging into ice water. The 2 by 2 slide may be purchased from a photography shop or some slide vendors can supply these. I have adapted one of the 30 slide racks for the 2 X 2 slides, had the guys in maintenance cut one side down and reassembled it, but some vendors also sell that size rack. If you need more details you may contact me. Shirley Powell -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chris Pomajzl Sent: Thursday, March 16, 2006 8:49 AM To: HISTONET Subject: [Histonet] Large Sections: Prostate One of our pathologists is interested in getting a whole section of a bisected prostate with subsequent staining. This would require very large glass slides and a special microtome (I would imagine). This is for research purposes. Does anyone have this capability, or do you have information on where it can be done? Thanks in advance. Chris Pomajzl, HTL (ASCP) Histology Supervisor Clinical Pathology Laboratories, Inc. 9200 Wall Street Austin, Texas 78754 512.873.1660 (o) 512.873.5004 (f) cpomajzl@cpllabs.com CONFIDENTIALITY NOTICE: This communication is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If you are not the intended recipient, you are notified that any use, dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this message in error, please notify the sender immediately and destroy this communication. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Maxim_71 <@t> mail.ru Thu Mar 16 09:15:57 2006 From: Maxim_71 <@t> mail.ru (Maxim Peshkov) Date: Thu Mar 16 09:23:17 2006 Subject: [Histonet] Mayer's Hematoxylin Message-ID: <112751109.20060316181557@mail.ru> Try follows: "Mayer - HCl Alum Hematoxylin Hematoxylin 1g Aluminum ammonium sulphate 50g Distilled water 1L Sodium iodate 0.2g Hydrochloric acid 1 mL Compounding procedure Dissolve the alum and hematoxylin in the water. Add the sodium iodate, and bring to the boil for a few minutes. Cool to room temperature and filter. Add the concentrated hydrochloric acid. Notes and comments The solution may be used immediately. It is stable for a few months only. Calcium deposits are removed. Staining time 5 - 10 minutes. Staining characteristics A progressive formulation. Gives very precise nuclear staining. No other structures are stained. Suggested use Counterstaining nuclei. Precisely nuclear H&E." For more detail address to Bryan Llewellin. (More thank You, Bryan!) This info I was found in www.Stainsfile.info on 2003 year. Now his no there. This recipe work very well for me over 1 year. In this solution I add 10 drops of Tween 20 per 1 L. This prevented form the surface film. Sincerely, Maxim Peshkov. Taganrog. Russia. maito: Maxim_71@mail.ru From rjbuesa <@t> yahoo.com Thu Mar 16 09:46:39 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 16 09:46:43 2006 Subject: [Histonet] Large Sections: Prostate In-Reply-To: <002c01c64900$63013910$26fca8c0@CSP> Message-ID: <20060316154639.61181.qmail@web61219.mail.yahoo.com> Chris: On a separate e-mail I am forwarding a photo file with all the components I made in our lab (cassettes, molds and holging blocks) to section whole prostates. At the start we used a regular rotary microtome with enough block clearance, but as the work increased we bought a Leitz sliding microtome. Ren? J. Chris Pomajzl wrote: One of our pathologists is interested in getting a whole section of a bisected prostate with subsequent staining. This would require very large glass slides and a special microtome (I would imagine). This is for research purposes. Does anyone have this capability, or do you have information on where it can be done? Thanks in advance. Chris Pomajzl, HTL (ASCP) Histology Supervisor Clinical Pathology Laboratories, Inc. 9200 Wall Street Austin, Texas 78754 512.873.1660 (o) 512.873.5004 (f) cpomajzl@cpllabs.com CONFIDENTIALITY NOTICE: This communication is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If you are not the intended recipient, you are notified that any use, dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this message in error, please notify the sender immediately and destroy this communication. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Mail Use Photomail to share photos without annoying attachments. From gcallis <@t> montana.edu Thu Mar 16 09:50:57 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Mar 16 09:51:07 2006 Subject: [Histonet] Large Sections: Prostate In-Reply-To: <01M04AVO2IRY8WZLNQ@Macon2.Mercer.edu> References: <002c01c64900$63013910$26fca8c0@CSP> <01M04AVO2IRY8WZLNQ@Macon2.Mercer.edu> Message-ID: <6.0.0.22.1.20060316083309.01b61b90@gemini.msu.montana.edu> Dear Shirley et al. I loved your description of how to work with large tissues!! Another embedding suggestion: If you have an oven set at 60C, preheat your wooden block (use hard wood and not soft pine) in the 60 C oven.. Then add paraffin to your preheated embedding mold, fill with paraffin, add tissue, then place a 60C preheated plastic cassette bottom on top of tissue as a spacer, then place the heated wooden block on top of the spacer. Remove this whole combination of things from oven and place on embedding center to harden paraffin or on top of a solid block of ice in a cake pan. We found this to be less messy than mounting a block holder into paraffin and very fast when dealing with numerous blocks. The wooden block will be firmly embedded in back of block to keep things very solid during sectioning. An excellent embedding mold is the L's with metal plate, you can shape the L's into block size you need, and embed outside an oven, try EM Sciences. Release of block is instantaneous after paraffin hardens. I have seen people use the ring style plastic molds in the same way to hold the block in a microtome, but the harder back is superior than something paraffin filled - more stable. Thermo Electron i.e. Shandon had an article on how to do this some years ago, you could ask them and look at the photos. There used to be hard rubber blocks and may still be available, check Electron Microscopy Sciences on the off chance they have these. They are excellent for large paraffin blocks, harder than soft wood. If I still had mine, I would send off to you - alas - long gone! We used to do this with large bone slabs (distal ends of sheep tibias) for sledge microtome sectioning. You can buy 2 x 2 slides from Brain Research and coverglasses too. Also Arthur Thomas has a huge selection of oversize coverglasses at various thicknesses. Check with Erie Scientific for oversize slides, we used to be able to buy large size from Fisher, but Thomas or Brain Research had them in catalogs. Good luck At 08:19 AM 3/16/2006, you wrote: >Hi Chris, > >Actually you can do this yourself, I have mounted the prostate gland on a 2 >by 2 slide if not too large. Make sure the knife clearance is adequate for >larger blocks on your microtome or it will not work. You will have to have >a sliding microtome for the job. The embedded block can be mounted on a >block of wood the size of the cassette to fit into your chuck. The tissue >is processed pretty much like your regular specimens if cut thin enough. >The specimen can be wrapped in lens paper used to clean scopes by stapling >the edges. After processing, use a slide box to embed in paraffin and cut >off the excess after solidified. Mount it on your wooden block by heating >the back of the paraffin block and submerging into ice water. The 2 by 2 >slide may be purchased from a photography shop or some slide vendors can >supply these. I have adapted one of the 30 slide racks for the 2 X 2 >slides, had the guys in maintenance cut one side down and reassembled it, >but some vendors also sell that size rack. If you need more details you may >contact me. > >Shirley Powell Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 From llewllew <@t> shaw.ca Thu Mar 16 09:59:02 2006 From: llewllew <@t> shaw.ca (Bryan Llewellyn) Date: Thu Mar 16 09:59:10 2006 Subject: [Histonet] Mayer's Hematoxylin References: <112751109.20060316181557@mail.ru> Message-ID: <002301c64912$8c033fe0$690a4246@yourlk4rlmsu> Thanks for the mention of StainsFile. The hemalum given is still there, but under the author's name. It is Krutsay's Hemalum. I had it in there twice, so removed the version without the identification. Look at http://stainsfile.info/StainsFile/stain/hematoxylin/aluminum/krutsay.htm Bryan Llewellyn ----- Original Message ----- From: "Maxim Peshkov" To: Cc: Sent: Thursday, March 16, 2006 7:15 AM Subject: Re: [Histonet] Mayer's Hematoxylin > Try follows: > > "Mayer - HCl > Alum Hematoxylin > Hematoxylin 1g > Aluminum ammonium sulphate 50g > Distilled water 1L > Sodium iodate 0.2g > Hydrochloric acid 1 mL > > Compounding procedure > Dissolve the alum and hematoxylin in the water. > Add the sodium iodate, and bring to the boil for > a few minutes. > Cool to room temperature and filter. > Add the concentrated hydrochloric acid. > > Notes and comments > The solution may be used immediately. > It is stable for a few months only. > Calcium deposits are removed. > > Staining time > 5 - 10 minutes. > > Staining characteristics > A progressive formulation. > Gives very precise nuclear staining. > No other structures are stained. > > Suggested use > Counterstaining nuclei. > Precisely nuclear H&E." > > For more detail address to Bryan Llewellin. > (More thank You, Bryan!) > This info I was found in www.Stainsfile.info on > 2003 year. Now his no there. > This recipe work very well for me over 1 year. > In this solution I add 10 drops of Tween 20 per 1 > L. This prevented form the surface film. > > Sincerely, > Maxim Peshkov. > Taganrog. > Russia. > maito: Maxim_71@mail.ru > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From dusko.trajkovic <@t> pfizer.com Thu Mar 16 10:34:59 2006 From: dusko.trajkovic <@t> pfizer.com (Trajkovic, Dusko) Date: Thu Mar 16 10:35:42 2006 Subject: [Histonet] Large Sections: Prostate Message-ID: <3AD0BD3142459B4E9B12CBEAFF2B89B2D897EC@lajamrexm01.amer.pfizer.com> I don't know how big the prostate specimen would be, but I have processed whole cross sections of adult dog brain in large cassettes from Surgipath. They also sell the embedding molds, 2x3 slides and coverglass. The only other thing you would need is an adjusting chuck to accommodate the large block. Dusko Trajkovic -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Thursday, March 16, 2006 7:51 AM To: Shirley Powell; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Large Sections: Prostate Dear Shirley et al. I loved your description of how to work with large tissues!! Another embedding suggestion: If you have an oven set at 60C, preheat your wooden block (use hard wood and not soft pine) in the 60 C oven.. Then add paraffin to your preheated embedding mold, fill with paraffin, add tissue, then place a 60C preheated plastic cassette bottom on top of tissue as a spacer, then place the heated wooden block on top of the spacer. Remove this whole combination of things from oven and place on embedding center to harden paraffin or on top of a solid block of ice in a cake pan. We found this to be less messy than mounting a block holder into paraffin and very fast when dealing with numerous blocks. The wooden block will be firmly embedded in back of block to keep things very solid during sectioning. An excellent embedding mold is the L's with metal plate, you can shape the L's into block size you need, and embed outside an oven, try EM Sciences. Release of block is instantaneous after paraffin hardens. I have seen people use the ring style plastic molds in the same way to hold the block in a microtome, but the harder back is superior than something paraffin filled - more stable. Thermo Electron i.e. Shandon had an article on how to do this some years ago, you could ask them and look at the photos. There used to be hard rubber blocks and may still be available, check Electron Microscopy Sciences on the off chance they have these. They are excellent for large paraffin blocks, harder than soft wood. If I still had mine, I would send off to you - alas - long gone! We used to do this with large bone slabs (distal ends of sheep tibias) for sledge microtome sectioning. You can buy 2 x 2 slides from Brain Research and coverglasses too. Also Arthur Thomas has a huge selection of oversize coverglasses at various thicknesses. Check with Erie Scientific for oversize slides, we used to be able to buy large size from Fisher, but Thomas or Brain Research had them in catalogs. Good luck At 08:19 AM 3/16/2006, you wrote: >Hi Chris, > >Actually you can do this yourself, I have mounted the prostate gland on a 2 >by 2 slide if not too large. Make sure the knife clearance is adequate for >larger blocks on your microtome or it will not work. You will have to have >a sliding microtome for the job. The embedded block can be mounted on a >block of wood the size of the cassette to fit into your chuck. The tissue >is processed pretty much like your regular specimens if cut thin enough. >The specimen can be wrapped in lens paper used to clean scopes by stapling >the edges. After processing, use a slide box to embed in paraffin and cut >off the excess after solidified. Mount it on your wooden block by heating >the back of the paraffin block and submerging into ice water. The 2 by 2 >slide may be purchased from a photography shop or some slide vendors can >supply these. I have adapted one of the 30 slide racks for the 2 X 2 >slides, had the guys in maintenance cut one side down and reassembled it, >but some vendors also sell that size rack. If you need more details you may >contact me. > >Shirley Powell Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately. From meint002 <@t> umn.edu Thu Mar 16 10:41:36 2006 From: meint002 <@t> umn.edu (meint002) Date: Thu Mar 16 10:41:40 2006 Subject: [Histonet] (no subject) Message-ID: <200603161641.k2GGfaIN018931@saturn.software.umn.edu> Dear Histos, I am trying to make a Gram's Iodine solution (1g iodine, 2g potassium iodide, 100ml distilled water to start)and I can't get the iodine to go into solution. If I use a stir bar the iodine is attracted to the magnet and doesn't come off. I've also tried heat and that doesn't seem to work either. All the text books I've looked at say shake until dissolved. Tried that, the iodine just sits in the bottom of the beaker and nothing. I don't remember having this problem before in school. All suggestions are welcome. Thanks in advance. Joyce Meints From kemlo.rogerson <@t> waht.swest.nhs.uk Thu Mar 16 10:55:32 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Mar 16 10:54:52 2006 Subject: [Histonet] (no subject) Message-ID: Dissolve the potassium iodide in 1 to 2 times its weight of water then the iodine will dissolve in the concentrated solution; when dissolved add the rest of the water. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 "Following the line of least resistance makes both men and rivers crooked" This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: meint002 [mailto:meint002@umn.edu] Sent: Thursday, March 16, 2006 4:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Dear Histos, I am trying to make a Gram's Iodine solution (1g iodine, 2g potassium iodide, 100ml distilled water to start)and I can't get the iodine to go into solution. If I use a stir bar the iodine is attracted to the magnet and doesn't come off. I've also tried heat and that doesn't seem to work either. All the text books I've looked at say shake until dissolved. Tried that, the iodine just sits in the bottom of the beaker and nothing. I don't remember having this problem before in school. All suggestions are welcome. Thanks in advance. Joyce Meints _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Thu Mar 16 11:11:47 2006 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Thu Mar 16 11:13:05 2006 Subject: [Histonet] QIHC Study Texts Message-ID: <84A42E6F38BE8A45AAB03628C8C80D12064FE9@dynams.dynacaremilwaukee.com> All, I have a tech that will be sitting for her QIHC qualification test and she wanted me to ask the histonet for appropriate text suggestions. All suggestions are welcome. Thank-you In Advance, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI From jkiernan <@t> uwo.ca Thu Mar 16 11:13:26 2006 From: jkiernan <@t> uwo.ca (John A. Kiernan) Date: Thu Mar 16 11:13:35 2006 Subject: [Histonet] (no subject) References: <200603161641.k2GGfaIN018931@saturn.software.umn.edu> Message-ID: <44199CB6.CC9667DC@uwo.ca> Could your "iodine" really be iron or magnetic iron oxide? Magnets don't pick up bits of iodine! Iodine is insoluble in water but it dissolves quite quickly in an aqueous solution of potassium iodide. It's good to see that you're correctly calling the solution Gram's iodine. Many people call it Lugol's, which is a stronger solution (6% iodine in 4% KI). -- ------------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan[AT]uwo.ca http://publish.uwo.ca/~jkiernan/ http://instruct.uwo.ca/anatomy/530/index.htm _______________________________ meint002 wrote: > > Dear Histos, > > I am trying to make a Gram's Iodine solution (1g iodine, 2g potassium iodide, > 100ml distilled water to start)and I can't get the iodine to go into > solution. If I use a stir bar the iodine is attracted to the magnet and > doesn't come off. I've also tried heat and that doesn't seem to work either. > All the text books I've looked at say shake until dissolved. Tried that, > the iodine just sits in the bottom of the beaker and nothing. > > I don't remember having this problem before in school. All suggestions are > welcome. Thanks in advance. > > Joyce Meints > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Karen.Heckford <@t> CHW.edu Thu Mar 16 11:12:43 2006 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Thu Mar 16 11:22:13 2006 Subject: [Histonet] VIP E300 Problems Message-ID: Good Morning Histonetters, I was wondering if anyone has had any problems getting replacement containers or any other parts for that matter for the VIP Tissue Processor E300. I am also experiencing a problem with water getting pulled out of my filter water and coming out of a hole where my last paraffin is placed. This has caused me a great deal of grief because my tissues are than infiltrated with water downed paraffin. This has happened at least three times. I have had Biomedical personnel fix the problem each time. Is it time to retire the old thing????? This just happened to me again and I now have a Migraine coming on!! Any suggestions!!!! Karen Heckford HT (ASCP) CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 1-415-668-1000 Ext. 6167 kheckfor@chw.edu From jfish <@t> gladstone.ucsf.edu Thu Mar 16 11:36:42 2006 From: jfish <@t> gladstone.ucsf.edu (Jo Dee Fish) Date: Thu Mar 16 11:36:49 2006 Subject: [Histonet] (no subject) In-Reply-To: <200603161641.k2GGfaIN018931@saturn.software.umn.edu> Message-ID: <000b01c64920$301bfec0$8903010a@JFISH> Joyce, When I make it up I shake the dry Iodine and dry Potassium Iodide together vigorously, then add the distilled water slowly while stirring with a stir bar until dissolved. It does take a while to fully dissolve. Good luck, Jo Dee Jo Dee Fish Research Technologist III Gladstone Institute of Cardiovascular Disease Telephone: (415) 734-2567 Fax: (415) 355-0230 E-mail: jfish@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of meint002 Sent: Thursday, March 16, 2006 8:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Dear Histos, I am trying to make a Gram's Iodine solution (1g iodine, 2g potassium iodide, 100ml distilled water to start)and I can't get the iodine to go into solution. If I use a stir bar the iodine is attracted to the magnet and doesn't come off. I've also tried heat and that doesn't seem to work either. All the text books I've looked at say shake until dissolved. Tried that, the iodine just sits in the bottom of the beaker and nothing. I don't remember having this problem before in school. All suggestions are welcome. Thanks in advance. Joyce Meints _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tfranzod <@t> dal.ca Thu Mar 16 11:42:04 2006 From: tfranzod <@t> dal.ca (Tamara Franz-Odendaal) Date: Thu Mar 16 11:42:27 2006 Subject: [Histonet] bone IHC Message-ID: <006101c64920$f678c990$6d20ad81@tam> Anyone with experience in collagen I staining on decalcified bone paraffin sections? Would this require more digestion than say collagen II on cartilage. any other differences? Collagen II is working perfectly but collagen I refuses to work. Thanks. Tamara Tamara Franz-Odendaal Biology Dept., Dalhousie University Halifax, NS, B3H 4J1 Canada From dsantana <@t> pmaonline.com Thu Mar 16 11:55:07 2006 From: dsantana <@t> pmaonline.com (Santana, Diane) Date: Thu Mar 16 11:57:56 2006 Subject: [Histonet] microscopes Message-ID: <4C96AA62BADFD81180CB0002A5AD67FB07113908@MAILPMA> I am looking at microscopes for kidney bx's. My dept will start assisting with the bx's after the bx has been removed. I have not done this in YEARS... then we watch the tissue, if it floated it was probably fat, if it sank to the bottom of the container it was more than likely tissue. So I am looking for a scope that I can see if I have tissue and some light reading as a refresher course. Thanks Diane From liz <@t> premierlab.com Thu Mar 16 11:57:58 2006 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Mar 16 11:58:07 2006 Subject: [Histonet] bone IHC In-Reply-To: <006101c64920$f678c990$6d20ad81@tam> Message-ID: <000c01c64923$29535d10$627e8a80@Chlipala> Tamara We use proteinase K digestion for Collagen I on formic acid decalcifed bone sections, we get our Collagen I from Biogenesis. For Collagen II we use no digestion and get our antibody from MD Biosciences. Its pricy but we find it works the best for us. I have tried the lab vision antibody and had some problems with it. I like the MD biosciences antibody because it's a rabbit polycolonal and a lot of our samples are generated in mouse, but we also run it on rat, sheep, and goat specimens. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tamara Franz-Odendaal Sent: Thursday, March 16, 2006 10:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] bone IHC Anyone with experience in collagen I staining on decalcified bone paraffin sections? Would this require more digestion than say collagen II on cartilage. any other differences? Collagen II is working perfectly but collagen I refuses to work. Thanks. Tamara Tamara Franz-Odendaal Biology Dept., Dalhousie University Halifax, NS, B3H 4J1 Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1.1446 (20060316) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From jcline <@t> wchsys.org Thu Mar 16 11:50:03 2006 From: jcline <@t> wchsys.org (Joyce Cline) Date: Thu Mar 16 12:06:33 2006 Subject: [Histonet] RE: Ventana Special Stains In-Reply-To: Message-ID: <000001c64922$117258a0$1d2a14ac@wchsys.org> I use the Ventana S.S. for our liver panel, what questions do you have? I have been through several problems with the staining quality. My work number is 301-665-4980. ________________________________________________________________________ __ Subject: [Histonet] Ventana Special Stains I'm looking for someone who is doing their PAS stains with the Ventana Special Stainer. I'm not happy with the way my liver bxs are looking and am looking for some advice. Thanks in advance. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From pwg1 <@t> cdc.gov Thu Mar 16 12:23:17 2006 From: pwg1 <@t> cdc.gov (Greer, Patricia) Date: Thu Mar 16 12:30:20 2006 Subject: [Histonet] (no subject) Message-ID: Joyce, In order to get the iodine into solution, first dissolve the 2g potassium iodide in 4-5 ml of water and dissolve the 1g of iodine in this. Dilute to 100 ml. These instructions are not often printed with the formula for making Gram's iodine and I remember how frustrating it was the first time I tried to make it up. Then I read the directions in Culling's "Handbook of Histopathological and Histochemical Techniques" Hope this helps. Pat Greer Infectious Disease Pathology Activity Centers for Disease Control and Prevention Mail Stop G-32 Atlanta, GA 30333 404-639-2811 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of meint002 Sent: Thursday, March 16, 2006 11:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Dear Histos, I am trying to make a Gram's Iodine solution (1g iodine, 2g potassium iodide, 100ml distilled water to start)and I can't get the iodine to go into solution. If I use a stir bar the iodine is attracted to the magnet and doesn't come off. I've also tried heat and that doesn't seem to work either. All the text books I've looked at say shake until dissolved. Tried that, the iodine just sits in the bottom of the beaker and nothing. I don't remember having this problem before in school. All suggestions are welcome. Thanks in advance. Joyce Meints _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Stacy_McLaughlin <@t> cooley-dickinson.org Thu Mar 16 12:30:20 2006 From: Stacy_McLaughlin <@t> cooley-dickinson.org (Stacy McLaughlin) Date: Thu Mar 16 12:30:27 2006 Subject: [Histonet] Microwave ovens Message-ID: Hi Everyone, I need info on vendors for microwave ovens used for special stains. What kinds are you using? Thanks in advance. Stacy Stacy McLaughlin HT (ASCP) Lead Tech, Histology Cooley Dickinson Hospital From cormier <@t> MIT.EDU Thu Mar 16 12:37:15 2006 From: cormier <@t> MIT.EDU (Kathy Cormier) Date: Thu Mar 16 12:37:25 2006 Subject: [Histonet] in situ hybridizers Message-ID: <000a01c64928$a5ecb1f0$92003712@mit.edu> Greetings 'Netters, We are currently looking into purchasing an in situ hybridizer. What are some of the features that we should be looking for? Fast ramp times? Small footprint? This is for research, so we need to be a bit flexible, but this is new territory for me, so I am vernturing into the unknown. Thanks for the help! Kathy DCM MIT From koellinr <@t> amgen.com Thu Mar 16 12:45:15 2006 From: koellinr <@t> amgen.com (Koelling, Ray) Date: Thu Mar 16 12:47:25 2006 Subject: [Histonet] (no subject) Message-ID: <16834C6DFFA6004C88DE4507FB8AE54403947C47@wa-mb4-sea.amgen.com> For those struggling to make up Gram's Iodine, you should look up what a chaotropic agent is (Potassium Iodide is one)and why then mixing things just as Pat wrote makes this so easy. Ray Raymond Koelling Research Scientist Pathology Amgen Corporation Seattle, WA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Greer, Patricia Sent: Thursday, March 16, 2006 10:23 AM To: meint002; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (no subject) Joyce, In order to get the iodine into solution, first dissolve the 2g potassium iodide in 4-5 ml of water and dissolve the 1g of iodine in this. Dilute to 100 ml. These instructions are not often printed with the formula for making Gram's iodine and I remember how frustrating it was the first time I tried to make it up. Then I read the directions in Culling's "Handbook of Histopathological and Histochemical Techniques" Hope this helps. Pat Greer Infectious Disease Pathology Activity Centers for Disease Control and Prevention Mail Stop G-32 Atlanta, GA 30333 404-639-2811 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of meint002 Sent: Thursday, March 16, 2006 11:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Dear Histos, I am trying to make a Gram's Iodine solution (1g iodine, 2g potassium iodide, 100ml distilled water to start)and I can't get the iodine to go into solution. If I use a stir bar the iodine is attracted to the magnet and doesn't come off. I've also tried heat and that doesn't seem to work either. All the text books I've looked at say shake until dissolved. Tried that, the iodine just sits in the bottom of the beaker and nothing. I don't remember having this problem before in school. All suggestions are welcome. Thanks in advance. Joyce Meints _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Stacy_McLaughlin <@t> cooley-dickinson.org Thu Mar 16 13:01:46 2006 From: Stacy_McLaughlin <@t> cooley-dickinson.org (Stacy McLaughlin) Date: Thu Mar 16 13:01:54 2006 Subject: [Histonet] Endogenous Biotin and CAP regulations Message-ID: Would anyone be willing to share how they address this issue, regarding CAP reg: ANP.22615 "If the laboratory uses an avidin-biotin complex (ABC) detection system (or a related system such as streptavidin-biotin or neutravidin-biotin), is there a policy that addresses nonspecific false positive staining from endogenous biotin? We're currently using the Ventana Nexes, with biotin-streptavidin detection system. Are there specific vendors your recommend for these reagents? Your answers are appreciated! Stacy Stacy McLaughlin HT (ASCP) Lead Tech, Histology Coolely Dickinson Hospital Stacy McLaughlin HT (ASCP) Lead Tech, Histology From jqb7 <@t> cdc.gov Thu Mar 16 13:03:20 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Thu Mar 16 13:12:13 2006 Subject: [Histonet] (no subject) Message-ID: We were all taught this back in histo. school......and Sheehan, while not giving the details as to why, describes the "recipe" much as Pat wrote. Jeanine Bartlett, BS, HT(ASCP) (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Koelling, Ray Sent: Thursday, March 16, 2006 1:45 PM To: Greer, Patricia; meint002; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (no subject) For those struggling to make up Gram's Iodine, you should look up what a chaotropic agent is (Potassium Iodide is one)and why then mixing things just as Pat wrote makes this so easy. Ray Raymond Koelling Research Scientist Pathology Amgen Corporation Seattle, WA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Greer, Patricia Sent: Thursday, March 16, 2006 10:23 AM To: meint002; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (no subject) Joyce, In order to get the iodine into solution, first dissolve the 2g potassium iodide in 4-5 ml of water and dissolve the 1g of iodine in this. Dilute to 100 ml. These instructions are not often printed with the formula for making Gram's iodine and I remember how frustrating it was the first time I tried to make it up. Then I read the directions in Culling's "Handbook of Histopathological and Histochemical Techniques" Hope this helps. Pat Greer Infectious Disease Pathology Activity Centers for Disease Control and Prevention Mail Stop G-32 Atlanta, GA 30333 404-639-2811 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of meint002 Sent: Thursday, March 16, 2006 11:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Dear Histos, I am trying to make a Gram's Iodine solution (1g iodine, 2g potassium iodide, 100ml distilled water to start)and I can't get the iodine to go into solution. If I use a stir bar the iodine is attracted to the magnet and doesn't come off. I've also tried heat and that doesn't seem to work either. All the text books I've looked at say shake until dissolved. Tried that, the iodine just sits in the bottom of the beaker and nothing. I don't remember having this problem before in school. All suggestions are welcome. Thanks in advance. Joyce Meints _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lrichey <@t> u.washington.edu Thu Mar 16 13:17:58 2006 From: lrichey <@t> u.washington.edu (Lori Richey) Date: Thu Mar 16 13:18:11 2006 Subject: [Histonet] Mayer's Hematoxylin In-Reply-To: References: Message-ID: <4419B9E6.4060604@u.washington.edu> Richard Allen makes a Mayer's Hematoxylin. It is included in the Congo Red kit that we buy, but I'm sure its available as a single item. Bauer, Karen wrote: >Hello to all, > >I'm looking for a Pre-Made Mayer's Hematoxylin that we can use for our routine H&E staining procedure. We make up our Mayer's Hematoxylin from scratch, but have recently been given permission to buy something that is pre-made. We've tried Harris, but the doctors do not like the differentiation that take place with using that kind of hematoxylin. (We stain by hand, so one tech's dip might be longer or shorter than another tech's dip in the differentiator.) They are seeing variations in nuclear staining, no matter how careful we are with our "dips". > >I'm looking for something that stains tissues in 5-10 minutes and does not need differentiation. Everything that I've tried so far is not working out to their satisfaction. We use a Gill's III for our FS set up, and they do not want that either (for the routine H&E's). > >Does anyone have any ideas or tips for me? I'll be greatly appreciative. > >Thanks in advance, > >Karen Bauer HT(ASCP) >Histology Supervisor >Luther Hospital >Eau Claire, WI >********************Confidentiality Notice******************** > > > >This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From cking <@t> rallansci.com Thu Mar 16 13:23:03 2006 From: cking <@t> rallansci.com (King, Curtis - RAS) Date: Thu Mar 16 13:23:10 2006 Subject: [Histonet] Mayer's Hematoxylin Message-ID: <490C1EC04BA10F4891494D0ED756AC9303C94D2E@usmi0012k03.rallansci.apogent.com> Hello All, I see there has been a lot of activity concerning the Richard Allan Scientific Hematoxylin. We do have a Modified Mayer's Hematoxylin cat#72804. Feel free to contact me with any questions regarding our complete reagent line. Curtis D. King, HT(ASCP) Tech Rite Consultant Richard Allan Scientific 4481 Campus Drive Kalamazoo, MI 49008 Phone: 800-522-7270 ext 672 Fax: 296-372-2734 E-Mail: cking@rallansci.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Lori Richey Sent: Thursday, March 16, 2006 2:18 PM To: Bauer, Karen Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Mayer's Hematoxylin Richard Allen makes a Mayer's Hematoxylin. It is included in the Congo Red kit that we buy, but I'm sure its available as a single item. Bauer, Karen wrote: >Hello to all, > >I'm looking for a Pre-Made Mayer's Hematoxylin that we can use for our routine H&E staining procedure. We make up our Mayer's Hematoxylin from scratch, but have recently been given permission to buy something that is pre-made. We've tried Harris, but the doctors do not like the differentiation that take place with using that kind of hematoxylin. (We stain by hand, so one tech's dip might be longer or shorter than another tech's dip in the differentiator.) They are seeing variations in nuclear staining, no matter how careful we are with our "dips". > >I'm looking for something that stains tissues in 5-10 minutes and does not need differentiation. Everything that I've tried so far is not working out to their satisfaction. We use a Gill's III for our FS set up, and they do not want that either (for the routine H&E's). > >Does anyone have any ideas or tips for me? I'll be greatly appreciative. > >Thanks in advance, > >Karen Bauer HT(ASCP) >Histology Supervisor >Luther Hospital >Eau Claire, WI >********************Confidentiality Notice******************** > > > >This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From eearle <@t> abrazohealth.com Thu Mar 16 13:23:34 2006 From: eearle <@t> abrazohealth.com (Earle, Elizabeth) Date: Thu Mar 16 13:23:40 2006 Subject: [Histonet] PowerPath Message-ID: <4727B24D9A8575479222635B57E3B3DB0183727B@mail.vhswest.local> Any PowerPath users, could you please email me directly? I have a lot of questions. Whether you were involved with setup and implementation or were/are a user at the bench, I could use as much information as anyone can share. Thanks Elizabeth From JurciseJ <@t> pediatrics.ohio-state.edu Thu Mar 16 13:27:06 2006 From: JurciseJ <@t> pediatrics.ohio-state.edu (Jurcisek, Joseph) Date: Thu Mar 16 13:28:19 2006 Subject: [Histonet] dual immunofluorescence Message-ID: <714B9F12B4E18C4C843B66E8E190F2ADB1ED4A@res2k3ms01.CRII.ORG> I would like to label some frozen sections with 2-3 antibodies directed against different antigenic sites. I would like to use fluorescence to identify these Abs. What is the best way to accomplish this. I have seen protocols where both primary Abs are mixed in a cocktail as well as protocols where the primary, secondary, and tertiary (if warranted)is completed for one antigen and then the process repeated for the second. I have run both of the Abs singly on similar tissue with decent results but now I would like to label the same tissue with both. Any help is greatly appreciated. Joe Joseph A. Jurcisek Senior Research Associate Center for Microbial Pathogenesis Columbus Children's Research Institute 700 Children's Dr. Columbus, OH 43205-2696 (614) 355-3521 fax (614) 722-2818 jurcisej@ccri.net From jcresor <@t> lcpath.com Thu Mar 16 14:34:57 2006 From: jcresor <@t> lcpath.com (Jennifer N. Cresor) Date: Thu Mar 16 14:35:06 2006 Subject: [Histonet] Sure-path Autocyte users Message-ID: <200603162035.k2GKZ1r21476@plus34.host4u.net> Hello, Does anyone coat their own slides with something besides Tripath slide coat? Who supplies it? Any info would be helplful, thanks! Jennifer jcresor@lcpath.com From mparker <@t> epl-inc.com Thu Mar 16 14:53:36 2006 From: mparker <@t> epl-inc.com (Mary Parker) Date: Thu Mar 16 14:53:43 2006 Subject: [Histonet] unsubscribe Message-ID: From akbitting <@t> geisinger.edu Thu Mar 16 15:34:45 2006 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Thu Mar 16 15:35:14 2006 Subject: [Histonet] Large Sections: Prostate Message-ID: We've been cutting whole mount prostates here for about a year now. Leica has a Supercassette clamp for their 2265 Microtome which will hold the Supercassettes from Surgipath. We mount our sections on slides we purchase from Brain Research Labs. We stain them using special baskets with adapters that will fit onto a Sakura 2000 or 601 stainer. Those are sold by Sakura. And finally, our coverglass is purchased from Brain Research Labs as well. BRL also sells cardboard slide folders for the larger slides. If I can be of any more help, please let me know. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From AnthonyH <@t> chw.edu.au Thu Mar 16 15:42:18 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Mar 16 15:42:30 2006 Subject: [Histonet] (no subject) Message-ID: Tro dissolve the iodine, make up your potassium iodide solution first. You will find that the iodine will dissolve quite readily in this. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of meint002 Sent: Friday, 17 March 2006 3:42 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Dear Histos, I am trying to make a Gram's Iodine solution (1g iodine, 2g potassium iodide, 100ml distilled water to start)and I can't get the iodine to go into solution. If I use a stir bar the iodine is attracted to the magnet and doesn't come off. I've also tried heat and that doesn't seem to work either. All the text books I've looked at say shake until dissolved. Tried that, the iodine just sits in the bottom of the beaker and nothing. I don't remember having this problem before in school. All suggestions are welcome. Thanks in advance. Joyce Meints _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From meint002 <@t> umn.edu Thu Mar 16 15:49:54 2006 From: meint002 <@t> umn.edu (meint002) Date: Thu Mar 16 15:49:59 2006 Subject: [Histonet] Gram's iodine mystery solved Message-ID: <200603162149.k2GLns4d019075@vanguard.software.umn.edu> Dear Histos, Thanks to all for your advice about making Gram's Iodine soln. There was something wrong about the reagent iodine I was using. I borrowed the proper iodine from another histo lab (thanks Luann) and now all is just fine. Dissolving it in the Potassium iodide worked like a charm. Joyce Meints Histologist University of Minnesota Paul and Sheila Wellstone Muscular Dystrophy Center MMC 206 420 Delaware St. SE Minneapolis, MN 55455-0392 e-mail: meint002@umn.edu lab phone: 612-626-4703 From joycejudge259 <@t> hotmail.com Thu Mar 16 16:11:13 2006 From: joycejudge259 <@t> hotmail.com (joyce judge) Date: Thu Mar 16 16:11:23 2006 Subject: [Histonet] TNF alpha Message-ID: I am looking for an antibody that is specific for Human TNF alpha and works well in FFPE tissue, any help would be appreciated. Joyce Judge Novartis From b003046 <@t> nf.au.dk Thu Mar 16 16:38:26 2006 From: b003046 <@t> nf.au.dk (b003046@nf.au.dk) Date: Thu Mar 16 16:38:34 2006 Subject: [Histonet] Cell survival Message-ID: <1142548706.4419e8e2440c3@webmail.nf.au.dk> Hi, I am looking for a way to detect cell survival in paraffin-embedded tissue in rats. Does anybody have a suggestion? Any help would be appreciated. kind regards Mette K. Hagensen From tpmorken <@t> labvision.com Thu Mar 16 16:57:06 2006 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Thu Mar 16 16:57:14 2006 Subject: [Histonet] California Society for Histotechnology Annual Symposium Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA20328D74D@usca0082k08.labvision.apogent.com> Details and registration forms for the California Society for Histotechnology's Annual Symposium on May 18-21 in Costs Mesa, CA can be downloaded from the CSH website: http://www.californiahistology.org/ Tim Morken From ploykasek <@t> phenopath.com Thu Mar 16 17:06:36 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Thu Mar 16 17:07:00 2006 Subject: [Histonet] Amyloid Message-ID: I am hoping someone can help. I'm looking for an antibody to fibrinogen A alpha chain. An antibody referenced in a NEJM June 2002 is no longer available. Thanks for the help. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From pruegg <@t> ihctech.net Thu Mar 16 17:30:51 2006 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Thu Mar 16 17:30:57 2006 Subject: [Histonet] Cd8 on rat tissue Message-ID: <200603162330.k2GNUhm6008348@chip.viawest.net> I was wondering what is being used for CD8 labeling of rat tissue? I assume that it still has to be done on frozen tissue? I have done a lot of rat anti-cd8 on mouse tissue using BD antibody but now I need to do it on rat tissue. Thanks, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 216 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From mbmphoto <@t> gmail.com Thu Mar 16 19:53:00 2006 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Thu Mar 16 19:52:35 2006 Subject: [Histonet] need anti-hrGFP polyclonal Antibody Message-ID: Can anyone recommend a good working primary antibody for anti-hrGFP (humanized Renilla Green Fluorescent protein) for primate & rodent brain tissue. Tissue has been fixed using an aldehyde fixative. Sections are cut at 40um for free-floating frozen sections. Please let me hear from vendors too. Any assistance you can provide will be greatly appreciated. Yours Maria Bartola Mejia University of California San Francisco (UCSF) Department of Neurological Surgery San Francisco, CA 94103 From clcses <@t> gmail.com Thu Mar 16 20:48:31 2006 From: clcses <@t> gmail.com (Carmen Contreras-Sesvold) Date: Thu Mar 16 20:48:36 2006 Subject: [Histonet] endogenous red autofluorescence quenching Message-ID: <46a3be380603161848u5fa8c38ck554abe3112ced585@mail.gmail.com> I am looking for a method to quench or block endogenous red autofluorescence. The sudan black information for quenching green autofluorecsence worked great!!! Thank you histonetters for all the advice. Greatfully, Carmen From jkiernan <@t> uwo.ca Thu Mar 16 22:42:40 2006 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Thu Mar 16 22:41:47 2006 Subject: [Histonet] Gram's iodine mystery solved In-Reply-To: <200603162149.k2GLns4d019075@vanguard.software.umn.edu> References: <200603162149.k2GLns4d019075@vanguard.software.umn.edu> Message-ID: <441A3E40.6000708@uwo.ca> Is this the end of the matter? Who swapped your iodine for iron filings? Do you have an enemy doing horrid things with the chemicals on your shelves? -- ------------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan[AT]uwo.ca http://publish.uwo.ca/~jkiernan/ http://instruct.uwo.ca/anatomy/530/index.htm _______________________________ meint002 wrote: > Dear Histos, > > Thanks to all for your advice about making Gram's Iodine soln. > > There was something wrong about the reagent iodine I was using. I borrowed > the proper iodine from another histo lab (thanks Luann) and now all is just > fine. Dissolving it in the Potassium iodide worked like a charm. > > > Joyce Meints > Histologist > > University of Minnesota > Paul and Sheila Wellstone Muscular Dystrophy Center > MMC 206 > 420 Delaware St. SE > Minneapolis, MN 55455-0392 > > e-mail: meint002@umn.edu > lab phone: 612-626-4703 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo.rogerson <@t> waht.swest.nhs.uk Fri Mar 17 02:23:21 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Mar 17 02:22:47 2006 Subject: [Histonet] (no subject) Message-ID: While I beg to differ; Gram Weigert's iodine is in fact 1g of iodine with 2g of potassium iodide in 100 mls dist water and is the beast in question. Gram's iodine is 1g of iodine with 2g of potassium iodide in 300 mls dist water and is therefore more dilute than 'meint200' wanted. Lugol's "rubefacient solution" is 1g of iodine with 2g of potassium iodide in 12 mls of dist water; as you say the stronger solution but I don't 'do' %. Test: Cowdry's iodine and Langeron's iodine, any idea? I did stifle the comment about magnetic iodine but I assume 'reagent' iodine is different over there. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 "Following the line of least resistance makes both men and rivers crooked" This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: John A. Kiernan [mailto:jkiernan@uwo.ca] Sent: Thursday, March 16, 2006 5:13 PM To: meint002; Histonet Subject: Re: [Histonet] (no subject) Could your "iodine" really be iron or magnetic iron oxide? Magnets don't pick up bits of iodine! Iodine is insoluble in water but it dissolves quite quickly in an aqueous solution of potassium iodide. It's good to see that you're correctly calling the solution Gram's iodine. Many people call it Lugol's, which is a stronger solution (6% iodine in 4% KI). -- ------------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan[AT]uwo.ca http://publish.uwo.ca/~jkiernan/ http://instruct.uwo.ca/anatomy/530/index.htm _______________________________ meint002 wrote: > > Dear Histos, > > I am trying to make a Gram's Iodine solution (1g iodine, 2g potassium iodide, > 100ml distilled water to start)and I can't get the iodine to go into > solution. If I use a stir bar the iodine is attracted to the magnet and > doesn't come off. I've also tried heat and that doesn't seem to work either. > All the text books I've looked at say shake until dissolved. Tried that, > the iodine just sits in the bottom of the beaker and nothing. > > I don't remember having this problem before in school. All suggestions are > welcome. Thanks in advance. > > Joyce Meints > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From abright <@t> brightinstruments.com Fri Mar 17 06:02:53 2006 From: abright <@t> brightinstruments.com (Alan Bright) Date: Fri Mar 17 05:53:34 2006 Subject: [Histonet] Mohs frozen Sections Message-ID: Many Thanks to those of you that kindly replied to my email below. Your information was most informative and will now enable me to complete my development which I am sure will be of great use to Histologist too. I will keep you informed when this development is completed and let you have full details. I would also welcome an answer from those of you that have not had a chance to reply yet. Happy St Patrick's Day Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com -----Original Message----- From: Alan Bright Sent: 15 March 2006 10:05 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mohs frozen Sections I am in the final stages of unique development to aid Mohs frozen sectioning. It would be of great assistance from those of you that carry out this study, if you could inform me if you have Liquid Nitrogen available in your laboratory? Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sharon.willman <@t> bms.com Fri Mar 17 06:10:05 2006 From: sharon.willman <@t> bms.com (Sharon E Willman) Date: Fri Mar 17 06:10:10 2006 Subject: [Histonet] Sudan Black Staining Message-ID: <441AA71D.2060900@bms.com> Hi, I am working on the Sudan Black stain. Do the slides need to be read immediately due to bleeding of the stain after approximately 24 hours? Thanks for any information you can give me about the stain. Sharon Willman From Rachael_Emerson <@t> URMC.Rochester.edu Fri Mar 17 06:42:38 2006 From: Rachael_Emerson <@t> URMC.Rochester.edu (Emerson, Rachael) Date: Fri Mar 17 06:42:55 2006 Subject: [Histonet] Double stain ? Message-ID: Hello. I am attempting to do a sequential double stain with 2 different antibodies. Unfortunately, one requires using Triton-X 100 for antigen retrieval and the other requires using trypsin. I've run trials using several different antigen retrieval methods and these are the only ones that work. I'm afraid that performing 2 different retrieval steps will cause the stain not to work. I would really appreciate any thoughts or suggestions. I am working with paraformaldehyde fixed mouse embryos. Thanks Rachael Emerson From Jackie.O'Connor <@t> abbott.com Fri Mar 17 06:51:56 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Mar 17 06:52:20 2006 Subject: [Histonet] Somewhat rhetorical question regarding antibody incubation time Message-ID: If you have an antibody that the manufacturer recommends overnight incubation at 4C at 1:1200 dilution - is there anything to be gained from increasing the concentration (1:600) and incubating for one hour RT instead? From susan.wells <@t> bms.com Fri Mar 17 06:53:19 2006 From: susan.wells <@t> bms.com (Susan Q Wells) Date: Fri Mar 17 06:56:29 2006 Subject: [Histonet] Cd8 on rat tissue In-Reply-To: <200603162330.k2GNUhm6008348@chip.viawest.net> References: <200603162330.k2GNUhm6008348@chip.viawest.net> Message-ID: <441AB13F.30805@bms.com> I have used mouse anti-rat from BD at .1ug/ml on frozen rat sections with Dako's LSAB2 rat kit (standard protocol) with good results. Patsy Ruegg wrote: >I was wondering what is being used for CD8 labeling of rat tissue? I assume >that it still has to be done on frozen tissue? I have done a lot of rat >anti-cd8 on mouse tissue using BD antibody but now I need to do it on rat >tissue. >Thanks, >Patsy > >Patsy Ruegg, HT(ASCP)QIHC >IHCtech, LLC >Fitzsimmons BioScience Park >12635 Montview Blvd. Suite 216 >Aurora, CO 80010 >P-720-859-4060 >F-720-859-4110 >wk email pruegg@ihctech.net >web site www.ihctech.net > > >This email is confidential and intended solely for the use of the Person(s) >('the intended recipient') to whom it was addressed. Any views or opinions >presented are solely those of the author. It may contain information that is >privileged & confidential within the meaning of applicable law. Accordingly >any dissemination, distribution, copying, or other use of this message, or >any of its contents, by any person other than the intended recipient may >constitute a breach of civil or criminal law and is strictly prohibited. If >you are NOT the intended recipient please contact the sender and dispose of >this e-mail as soon as possible. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Doug.Geddes <@t> lhsc.on.ca Fri Mar 17 07:06:53 2006 From: Doug.Geddes <@t> lhsc.on.ca (Doug Geddes) Date: Fri Mar 17 07:07:40 2006 Subject: [Histonet] Commercial micro waves Message-ID: Would anyone be will to share any information good or bad about commercial mircro waves for antigen retrieval, vendors welcomed. Doug Geddes BSc., MLT Dept of Pathology London Health Sciences Centre London, Ontario, Canada ----------------------------------------- This information is directed in confidence solely to the person named above and may contain confidential and/or privileged material. This information may not otherwise be distributed, copied or disclosed. If you have received this e-mail in error, please notify the sender immediately via a return e-mail and destroy original message. Thank you for your cooperation. From ROrr <@t> enh.org Fri Mar 17 07:32:28 2006 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Fri Mar 17 07:32:34 2006 Subject: [Histonet] RE: Histonet Digest, Vol 28, Issue 27 Message-ID: Stacy, I wrote a sentence describing the issues of background staining. I then described what detection kits we use in our lab. We use both polymer(Dako/Biocare) and the Ventana I view on the benchmark. I also added instructions to determine if Endogenous Biotin is evident (omitting primary and secondary and just adding the label and chromogen on one slide, just the chromogen on a second slide...) I put something in there about following manufacturer's recommendations on the data sheets. I addressed protein blocks and endogenous peroxidase all in the same section and called it "Blocking Reagents". I was just inspected in February and the inspectors and I scrutinized that regulation especially since it was new. I passed so I'm thinking what I wrote was adequate. I'll go ahead and send you the excerpt with my references in a separate file. Let me know if you can't receive files from strangers. I can fax if you'd like Have a great weekend Becky Becky Orr CLA,HT(ASCP) IHC Lead Evanston Northwestern Healthcare 847-570-2771 - Message: 6 Date: Thu, 16 Mar 2006 14:01:46 -0500 From: "Stacy McLaughlin" Subject: [Histonet] Endogenous Biotin and CAP regulations To: Message-ID: Content-Type: text/plain; charset="us-ascii" Would anyone be willing to share how they address this issue, regarding CAP reg: ANP.22615 "If the laboratory uses an avidin-biotin complex (ABC) detection system (or a related system such as streptavidin-biotin or neutravidin-biotin), is there a policy that addresses nonspecific false positive staining from endogenous biotin? We're currently using the Ventana Nexes, with biotin-streptavidin detection system. Are there specific vendors your recommend for these reagents? Your answers are appreciated! Stacy Stacy McLaughlin HT (ASCP) Lead Tech, Histology Coolely Dickinson Hospital Stacy McLaughlin HT (ASCP) Lead Tech, Histology From ROrr <@t> enh.org Fri Mar 17 08:03:28 2006 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Fri Mar 17 08:03:33 2006 Subject: [Histonet] double stain Message-ID: Rachel, I have encountered similar issues in my journey through doublestaining. >From what I know of Triton X, it's mainly a surfactant. I'm not sure of the pH. There are solutions on the market that most likely have some type of surfactant in them, hence all the soapy bubbles in the rinsing. What solutions have you tried? What I have been successful with in this type scenario is trying a combination of either a lighter (less harsh HEIR) and then either a diluted enzyme or less time in the enzyme. Can you alter the concentration of the antibodies? One using the triton might need higher concentration, or more time. When you say sequentially, are you taking one ab all the way to the chromogen then starting the second one? I would imagine this is your protocol, but that would be good to know. Becky Orr CLA,HT(ASCP) IHC Lead Evanston Northwestern Healthcare 847-570-2771 Message: 28 Date: Fri, 17 Mar 2006 07:42:38 -0500 From: "Emerson, Rachael" Subject: [Histonet] Double stain ? To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello. I am attempting to do a sequential double stain with 2 different antibodies. Unfortunately, one requires using Triton-X 100 for antigen retrieval and the other requires using trypsin. I've run trials using several different antigen retrieval methods and these are the only ones that work. I'm afraid that performing 2 different retrieval steps will cause the stain not to work. I would really appreciate any thoughts or suggestions. I am working with paraformaldehyde fixed mouse embryos. Thanks Rachael Emerson ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 28, Issue 27 **************************************** From PMcArdle <@t> ebsciences.com Fri Mar 17 08:10:51 2006 From: PMcArdle <@t> ebsciences.com (Phil McArdle) Date: Fri Mar 17 08:11:04 2006 Subject: [Histonet] Commercial micro waves In-Reply-To: References: Message-ID: <441AC36B.70202@ebsciences.com> Hi: Check out http://www.ebsstore.com/control/category/~category_id=C Please feel free to contact me with any questions or issues you may have. Best regards, Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. Tel: 800.992.9037 x 341 Cell: 860.597.6796 Fax: 860.653.0422 PMcardle@ebsciences.com www.ebsciences.com "ADDING BRILLIANCE TO YOUR VISION" "I hate quotations. Tell me what you know." Ralph Waldo Emerson Doug Geddes wrote: > Would anyone be will to share any information good or bad about > commercial mircro waves for antigen retrieval, vendors welcomed. > > > Doug Geddes BSc., MLT > Dept of Pathology > London Health Sciences Centre > London, Ontario, Canada > > ----------------------------------------- > This information is directed in confidence solely to the person > named above and may contain confidential and/or privileged > material. This information may not otherwise be distributed, > copied or disclosed. If you have received this e-mail in error, > please notify the sender immediately via a return e-mail and > destroy original message. Thank you for your cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ROrr <@t> enh.org Fri Mar 17 09:04:02 2006 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Fri Mar 17 09:04:18 2006 Subject: [Histonet] Training Med Techs Message-ID: Hello everyone. I would appreciate any feedback from those of you who may have had to train MT's (ASCP) to work in Histology. They would be trained as histo techs with the intent to promote them into Anatomic Pathology (Histology) management positions. Candid comments welcome, especially from MT's who now work in histology! To me it would be like trying to train a policeman to be a fireman, it's a career, not a job, right? We see a HT shortage in the Chicago area, but I am unsure how to address this. Degreed individuals have proven critical thinking skills via a traditional education pathway, so I see the advantages, but to ignore very capable HT managers with proven management and organizational skills via non traditional pathways is becoming an issue with me. I mean it's not like Non degreed HT's are stooopid or something. Thank you Becky Becky Orr CLA,HT(ASCP) IHC Lead Evanston Northwestern Healthcare 847-570-2771 From rjbuesa <@t> yahoo.com Fri Mar 17 09:04:50 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 17 09:04:55 2006 Subject: [Histonet] Somewhat rhetorical question regarding antibody incubation time In-Reply-To: Message-ID: <20060317150450.43863.qmail@web61217.mail.yahoo.com> If it works it will expedite your TAT and I would certainly recommend that approach, just one thing though: you cannot equate a 2:1 increased concentration with a 16:1 reduction of incubation time + a 6:1 fold increase of temperature. You should make several tests to find out the correct new dilution factor that will allow you to change the incubation time and temperature to what you desire. Hope this will help you! Ren? J. Jackie M O'Connor wrote: If you have an antibody that the manufacturer recommends overnight incubation at 4C at 1:1200 dilution - is there anything to be gained from increasing the concentration (1:600) and incubating for one hour RT instead? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Travel Find great deals to the top 10 hottest destinations! From KDwyer3322 <@t> aol.com Fri Mar 17 09:15:43 2006 From: KDwyer3322 <@t> aol.com (KDwyer3322@aol.com) Date: Fri Mar 17 09:15:54 2006 Subject: [Histonet] New NY regulations Message-ID: <22b.87e55fa.314c2c9f@aol.com> Histonetters from New York: As stated below will the new regulations affect histotechnicians? Any help would be appreciated. Thanks, Kathy Dwyer The New York State Office of the Professions has posted updated information on our state licencing of clinical laboratory technologists,? technicians and Cytotechnologists as pasted below the direct link. http://www.op.nysed.gov/clp-mar06update.htm March 2006 The State Education Department, with the assistance of the State Board for Clinical Laboratory Technology and interested parties are in the process of developing draft regulations that will be necessary for the licensure of the professionals as clinical laboratory technologists, Cytotechnologists, and clinical laboratory technicians. Once these draft regulations are completed, they will be submitted to all interested parties and published in the State Register for comment. At the end of this process, regulations will be presented for consideration and action by the Board of Regents. It is anticipated that this process will be completed by mid-summer in 2006. Applications packets for licensure will not be available until the regulations are enacted. Prior to the enactment of the regulations, the Department cannot provide any specific information to individuals regarding licensure under the "Special Provisions" or "grandparenting.." As soon as the regulations are enacted, this information will be available. As soon as the regulations are enacted by the State Board of Regents, the Department will publish a Guide to Practice for the professions of Clinical Laboratory Technology, Clinical Laboratory Technician, and Cytotechnology, as well as an application packet for these professions. When they are available, the applications will be published on this website and can be downloaded and submitted to the Department. Applicants who wish to receive a hard copy of the Guide to Practice and the Application Packet may submit an e-mail request for the information now, but should not expect to receive this information before the summer of 2006. A notice will be put on this website when the applications are ready to be mailed. Clinical Laboratory Technicians, Clinical Laboratory Technologists and Cytotechnologists who want to request to be placed on a mailing list now for a hard copy of the application packet and receive it in the summer of 2006 should send an e-mail to OPFORMS@mail.nysed.gov, giving their name and address, and specify the packet they want by: Code 92A and profession title: Clinical Laboratory Technologist/Clinical Laboratory Technician, or Code 93A and profession title: Cytotechnology. http://www.op.nysed.gov/clp-mar06update.htm Page last updated: 03/15/2006 12:07:36 From MElliott <@t> mrl.ubc.ca Fri Mar 17 09:20:07 2006 From: MElliott <@t> mrl.ubc.ca (Mark Elliott) Date: Fri Mar 17 09:20:35 2006 Subject: [Histonet] Region IX Education Day Message-ID: <441A6327020000D60000464E@mail.mrl.ubc.ca> Hi everyone For anyone wanting an excuse to visit the wonderful city of Montreal, we have the perfect reason-Region IX is hosting an Education Day on May 6th, 2006 in this vibrant city. We are having 5 speakers on a variety of topics and 13 vendors will be present showcasing their products. Registration deadline is April 14th. Please visit http://www.nshregionix.org/education.html for more information, including registration forms, speaker schedule and hotel information. Hope to see you there! Mark Elliott Region IX Education Committee Chair From Eric.C.Kellar <@t> questdiagnostics.com Fri Mar 17 09:24:16 2006 From: Eric.C.Kellar <@t> questdiagnostics.com (Kellar, Eric C) Date: Fri Mar 17 09:24:33 2006 Subject: [Histonet] RE: Sudan Black Staining Message-ID: <6843061CE6B98E4B96590D4F299618F801583BB6@qdcws0117.us.qdx.com> To slow Sudan Black B (a nonionic, hydrophobic dye) 'bleeding' on unfixed cryostat sections, try running a duplicate slide and compare with this method: 1. Air dry duplicate slide after staining. 2. Clear slide through 3 changes of fresh Xylene or Xylene Substitute. 3. Glass coverslip using a permanent mounting media. The synthetic mountant will eventually dissolve some of the dye-lipid complex, but it will give you a longer window of time to microscopically examine your slides. Eric C. Kellar Histology/Immunohistochemistry Quest Diagnostics Inc. Miami Hi, I am working on the Sudan Black stain. Do the slides need to be read immediately due to bleeding of the stain after approximately 24 hours? Thanks for any information you can give me about the stain. Sharon Willman ============================================================================== The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. ============================================================================== From ROrr <@t> enh.org Fri Mar 17 09:27:50 2006 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Fri Mar 17 09:27:54 2006 Subject: [Histonet] clarifying the MT -HT training Message-ID: In clarification, I don't think I worded that right. It's a salary compensation issue. A non degreed HT is expected to take on the same responsibilities as a histology supervisor for 10K less than a trained MT crossing over to HT. So to reiterate, it's not so much being eligible to handle to management, it's about taking less salary for the same responsibility. Thank you all for the feedback this is seems like a warm issue! Becky Orr CLA,HT(ASCP) IHC Lead Evanston Northwestern Healthcare 847-570-2771 From asmith <@t> mail.barry.edu Fri Mar 17 09:35:48 2006 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Mar 17 09:35:53 2006 Subject: [Histonet] Gram's iodine mystery solved Message-ID: <5D2189E74151CC42BEC02906BA8996322B919F@exchsrv01.barrynet.barry.edu> Shockingly, labels are not always correct. I have a jar labelled "indigocarmine" whose contents is insoluble in water. I once (20 years ago) opened a brand new bottle labelled "acetic anhydride" and caught a whiff of isoamyl acetate. The contents also boiled at the boiling point of isoamyl acetate (4 C higher than acetic anhydride), and it did not dissolve in hot water. I have also borrowed a bottle of bromine from a colleague (25 years ago at another school) and found that it performed brominations fairly well, but required an elevated temperature to do them. I never did find out what the contaminant was or how it got there. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Kiernan Sent: Thursday, March 16, 2006 11:43 PM To: meint002 Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Gram's iodine mystery solved Is this the end of the matter? Who swapped your iodine for iron filings? Do you have an enemy doing horrid things with the chemicals on your shelves? -- ------------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan[AT]uwo.ca http://publish.uwo.ca/~jkiernan/ http://instruct.uwo.ca/anatomy/530/index.htm _______________________________ meint002 wrote: > Dear Histos, > > Thanks to all for your advice about making Gram's Iodine soln. > > There was something wrong about the reagent iodine I was using. I borrowed > the proper iodine from another histo lab (thanks Luann) and now all is just > fine. Dissolving it in the Potassium iodide worked like a charm. > > > Joyce Meints > Histologist > > University of Minnesota > Paul and Sheila Wellstone Muscular Dystrophy Center > MMC 206 > 420 Delaware St. SE > Minneapolis, MN 55455-0392 > > e-mail: meint002@umn.edu > lab phone: 612-626-4703 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From gcallis <@t> montana.edu Fri Mar 17 10:04:50 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Mar 17 10:04:56 2006 Subject: An aside on viewing kidney biopsies Re: [Histonet] microscopes In-Reply-To: <4C96AA62BADFD81180CB0002A5AD67FB07113908@MAILPMA> References: <4C96AA62BADFD81180CB0002A5AD67FB07113908@MAILPMA> Message-ID: <6.0.0.22.1.20060317090020.01b46438@gemini.msu.montana.edu> We supplied laboratories with slides that have the wells in them, commonly used for viewing larger creatures like insects, etc. These are sold by Fisher, etc The biospy was floated in PBS, and you can actually use any microscope at lowest power or stereomicroscope to look for glomeruli. This prevented crushing and flattening the biopsy, a problem we encountered far to many times when bx was examined between a slide and coverslip immediately after removal. At 10:55 AM 3/16/2006, you wrote: >I am looking at microscopes for kidney bx's. My dept will start assisting >with the bx's after the bx has been removed. I have not done this in >YEARS... then we watch the tissue, if it floated it was probably fat, if it >sank to the bottom of the container it was more than likely tissue. So I am >looking for a scope that I can see if I have tissue and some light reading >as a refresher course. Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 From jladuc <@t> trudeauinstitute.org Fri Mar 17 10:23:54 2006 From: jladuc <@t> trudeauinstitute.org (Judith LaDuc) Date: Fri Mar 17 10:17:22 2006 Subject: [Histonet] New NY regulations In-Reply-To: <22b.87e55fa.314c2c9f@aol.com> References: <22b.87e55fa.314c2c9f@aol.com> Message-ID: <441A9AB5.2A60.00B8.0@trudeauinstitute.org> Hi Kathy and NY Histotechicians/technologists, Yes, the new regulations will affect us. We are classified under the heading of clinical laboratory technicians/technologists and are subject to licensure. I would recommend signing up for the mailing list and checking into the website routinely. The following is a post from the Histonet archives that has a few links and a timeline of events. http://www.histosearch.com/histonet/Jan06/HistonetNYSLicensure.html Dr. Kathleen Doyle from NYS will be presenting an informational session at the Region 1 meeting in Saratoga Springs on Friday, April 28th. The Region 1 program (April 28th & 29th) can be accessed at http://www.nyhisto.org Judy LaDuc, BS HTL ASCP President, NYS Histotechnological Society >>> 03/17/06 10:15 am >>> Histonetters from New York: As stated below will the new regulations affect histotechnicians? Any help would be appreciated. Thanks, Kathy Dwyer The New York State Office of the Professions has posted updated information on our state licencing of clinical laboratory technologists, technicians and Cytotechnologists as pasted below the direct link. http://www.op.nysed.gov/clp- mar06update.htm March 2006 The State Education Department, with the assistance of the State Board for Clinical Laboratory Technology and interested parties are in the process of developing draft regulations that will be necessary for the licensure of the professionals as clinical laboratory technologists, Cytotechnologists, and clinical laboratory technicians. Once these draft regulations are completed, they will be submitted to all interested parties and published in the State Register for comment. At the end of this process, regulations will be presented for consideration and action by the Board of Regents. It is anticipated that this process will be completed by mid- summer in 2006. Applications packets for licensure will not be available until the regulations are enacted. Prior to the enactment of the regulations, the Department cannot provide any specific information to individuals regarding licensure under the "Special Provisions" or "grandparenting.." As soon as the regulations are enacted, this information will be available. As soon as the regulations are enacted by the State Board of Regents, the Department will publish a Guide to Practice for the professions of Clinical Laboratory Technology, Clinical Laboratory Technician, and Cytotechnology, as well as an application packet for these professions. When they are available, the applications will be published on this website and can be downloaded and submitted to the Department. Applicants who wish to receive a hard copy of the Guide to Practice and the Application Packet may submit an e- mail request for the information now, but should not expect to receive this information before the summer of 2006. A notice will be put on this website when the applications are ready to be mailed. Clinical Laboratory Technicians, Clinical Laboratory Technologists and Cytotechnologists who want to request to be placed on a mailing list now for a hard copy of the application packet and receive it in the summer of 2006 should send an e- mail to OPFORMS@mail.nysed.gov, giving their name and address, and specify the packet they want by: Code 92A and profession title: Clinical Laboratory Technologist/Clinical Laboratory Technician, or Code 93A and profession title: Cytotechnology. http://www.op.nysed.gov/clp- mar06update.htm Page last updated: 03/15/2006 12:07:36 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From detmar <@t> mshri.on.ca Fri Mar 17 10:34:15 2006 From: detmar <@t> mshri.on.ca (Jacqui Detmar) Date: Fri Mar 17 10:34:22 2006 Subject: [Histonet] immunohistochemistry and TUNEL background staining Message-ID: Hi all. I have been doing IHC and TUNELs on mouse placental tissues for the past 4 years and have been having problems with oodles of background staining during the past 6 months or so. I have tried doing avidin-biotin and streptavidin-biotin blocking and also using ABC (purchased from Vector) in high pH (TBS, pH 9.4) or high salt (TBS [NaCl = 0.5M], pH 7.6) solutions. Nothing seems to be working. The IHC using biotinylated secondary antibodies and the TUNEL is done using biotinylated dUTP. I have tried using old sections that I *know* have worked in the past and I am getting the same type of background staining as my newer blocks (i.e. the problem is likely not due to fixation (always the same...24 hours in 10% phosphate-buffered formalin), nor due to differences in paraffin embedding). So, could my xylene and ethanol solutions be a problem? We get the ethanol in-house here at the hospital and the Xylene is purchased through Fisher (X16-4). What about differences in TBS or PBS (I have tried using both to see if the problem would go away and it doesn't make a difference). Any advice would be appreciated. Regards, Jacqui Detmar, Ph.D. student Samuel Lunenfeld Research Institute Mount Sinai Hospital, Toronto, Ontario, Canada From jkiernan <@t> uwo.ca Fri Mar 17 11:03:44 2006 From: jkiernan <@t> uwo.ca (John A. Kiernan) Date: Fri Mar 17 11:03:50 2006 Subject: [Histonet] Sudan Black Staining References: <441AA71D.2060900@bms.com> Message-ID: <441AEBF0.58CE6C9C@uwo.ca> Sudan black B shouldn't bleed. After staining, rinse in 70% alcohol until non-lipid structures are destained, wash in water, and mount in an aqueous medium. The slides are good for months - probably longer. -- ------------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan[AT]uwo.ca http://publish.uwo.ca/~jkiernan/ http://instruct.uwo.ca/anatomy/530/index.htm _______________________________ Sharon E Willman wrote: > > Hi, > I am working on the Sudan Black stain. Do the slides need to be read > immediately due to bleeding of the stain after approximately 24 hours? > > Thanks for any information you can give me about the stain. > Sharon Willman > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Fri Mar 17 12:02:59 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Mar 17 12:03:11 2006 Subject: [Histonet] Somewhat rhetorical question regarding antibody incubation time In-Reply-To: References: Message-ID: <6.0.0.22.1.20060317105958.01bb2be0@gemini.msu.montana.edu> Jackie, Probably depends on what kind of fixation used too? PFA? NBF? It is always fun to try to beat a recommended time frame with a dilution panel starting at 10 ug/ml and/or 4 hrs instead of overnight. So many options to play with - At 05:51 AM 3/17/2006, you wrote: >If you have an antibody that the manufacturer recommends overnight >incubation at 4C at 1:1200 dilution - >is there anything to be gained from increasing the concentration (1:600) >and incubating for one hour RT instead? Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 From tfranzod <@t> dal.ca Fri Mar 17 12:28:31 2006 From: tfranzod <@t> dal.ca (Tamara Franz-Odendaal) Date: Fri Mar 17 12:29:02 2006 Subject: [Histonet] Re: question regarding antibody incubation time References: <200603171809.k2HI9Ztd011980@KIL-MX-1.UCIS.Dal.Ca> Message-ID: <00da01c649f0$9debe8e0$6d20ad81@tam> In response to the query of altering antiobdy dilution and reducing time. In my experience, a dilution of 1:1200 at 4C overnight is equivalent to 1:1200 for 1 hour at 37C. Increasing the concentration may create background staining problems despite the change in temp. I routinely use the same dilution overnight at 4C as I do for 1 hour at 37C with the same results. This said a dilution of 1:1200 sounds extreme to me, but if this is what the manufacturer recommends for the method you are using (IHC not western or ELISA) then this is a good starting point. Tamara Dr. Tamara Franz-Odendaal Biology Dept., Dalhousie University Halifax, NS, B3H 4J1 Canada ----- Original Message ----- From: To: Sent: Friday, March 17, 2006 2:09 PM Subject: Histonet Digest, Vol 28, Issue 28 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Somewhat rhetorical question regarding antibody incubation time (Jackie M O'Connor) 2. Re: Cd8 on rat tissue (Susan Q Wells) 3. Commercial micro waves (Doug Geddes) 4. RE: Histonet Digest, Vol 28, Issue 27 (Orr, Rebecca) 5. double stain (Orr, Rebecca) 6. Re: Commercial micro waves (Phil McArdle) 7. Training Med Techs (Orr, Rebecca) 8. Re: Somewhat rhetorical question regarding antibody incubation time (Rene J Buesa) 9. New NY regulations (KDwyer3322@aol.com) 10. Region IX Education Day (Mark Elliott) 11. RE: Sudan Black Staining (Kellar, Eric C) 12. clarifying the MT -HT training (Orr, Rebecca) 13. RE: Gram's iodine mystery solved (Smith, Allen) 14. An aside on viewing kidney biopsies Re: [Histonet] microscopes (Gayle Callis) 15. Re: New NY regulations (Judith LaDuc) 16. immunohistochemistry and TUNEL background staining (Jacqui Detmar) 17. Re: Sudan Black Staining (John A. Kiernan) ---------------------------------------------------------------------- Message: 1 Date: Fri, 17 Mar 2006 06:51:56 -0600 From: "Jackie M O'Connor" Subject: [Histonet] Somewhat rhetorical question regarding antibody incubation time To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="us-ascii" If you have an antibody that the manufacturer recommends overnight incubation at 4C at 1:1200 dilution - is there anything to be gained from increasing the concentration (1:600) and incubating for one hour RT instead? ------------------------------ Message: 2 Date: Fri, 17 Mar 2006 07:53:19 -0500 From: Susan Q Wells Subject: Re: [Histonet] Cd8 on rat tissue To: Patsy Ruegg Cc: histonet@pathology.swmed.edu Message-ID: <441AB13F.30805@bms.com> Content-Type: text/plain; format=flowed; charset=us-ascii I have used mouse anti-rat from BD at .1ug/ml on frozen rat sections with Dako's LSAB2 rat kit (standard protocol) with good results. Patsy Ruegg wrote: >I was wondering what is being used for CD8 labeling of rat tissue? I assume >that it still has to be done on frozen tissue? I have done a lot of rat >anti-cd8 on mouse tissue using BD antibody but now I need to do it on rat >tissue. >Thanks, >Patsy > >Patsy Ruegg, HT(ASCP)QIHC >IHCtech, LLC >Fitzsimmons BioScience Park >12635 Montview Blvd. Suite 216 >Aurora, CO 80010 >P-720-859-4060 >F-720-859-4110 >wk email pruegg@ihctech.net >web site www.ihctech.net > > >This email is confidential and intended solely for the use of the Person(s) >('the intended recipient') to whom it was addressed. Any views or opinions >presented are solely those of the author. It may contain information that is >privileged & confidential within the meaning of applicable law. Accordingly >any dissemination, distribution, copying, or other use of this message, or >any of its contents, by any person other than the intended recipient may >constitute a breach of civil or criminal law and is strictly prohibited. If >you are NOT the intended recipient please contact the sender and dispose of >this e-mail as soon as possible. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 3 Date: Fri, 17 Mar 2006 08:06:53 -0500 From: "Doug Geddes" Subject: [Histonet] Commercial micro waves To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Would anyone be will to share any information good or bad about commercial mircro waves for antigen retrieval, vendors welcomed. Doug Geddes BSc., MLT Dept of Pathology London Health Sciences Centre London, Ontario, Canada ----------------------------------------- This information is directed in confidence solely to the person named above and may contain confidential and/or privileged material. This information may not otherwise be distributed, copied or disclosed. If you have received this e-mail in error, please notify the sender immediately via a return e-mail and destroy original message. Thank you for your cooperation. ------------------------------ Message: 4 Date: Fri, 17 Mar 2006 07:32:28 -0600 From: "Orr, Rebecca" Subject: [Histonet] RE: Histonet Digest, Vol 28, Issue 27 To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Stacy, I wrote a sentence describing the issues of background staining. I then described what detection kits we use in our lab. We use both polymer(Dako/Biocare) and the Ventana I view on the benchmark. I also added instructions to determine if Endogenous Biotin is evident (omitting primary and secondary and just adding the label and chromogen on one slide, just the chromogen on a second slide...) I put something in there about following manufacturer's recommendations on the data sheets. I addressed protein blocks and endogenous peroxidase all in the same section and called it "Blocking Reagents". I was just inspected in February and the inspectors and I scrutinized that regulation especially since it was new. I passed so I'm thinking what I wrote was adequate. I'll go ahead and send you the excerpt with my references in a separate file. Let me know if you can't receive files from strangers. I can fax if you'd like Have a great weekend Becky Becky Orr CLA,HT(ASCP) IHC Lead Evanston Northwestern Healthcare 847-570-2771 - Message: 6 Date: Thu, 16 Mar 2006 14:01:46 -0500 From: "Stacy McLaughlin" Subject: [Histonet] Endogenous Biotin and CAP regulations To: Message-ID: Content-Type: text/plain; charset="us-ascii" Would anyone be willing to share how they address this issue, regarding CAP reg: ANP.22615 "If the laboratory uses an avidin-biotin complex (ABC) detection system (or a related system such as streptavidin-biotin or neutravidin-biotin), is there a policy that addresses nonspecific false positive staining from endogenous biotin? We're currently using the Ventana Nexes, with biotin-streptavidin detection system. Are there specific vendors your recommend for these reagents? Your answers are appreciated! Stacy Stacy McLaughlin HT (ASCP) Lead Tech, Histology Coolely Dickinson Hospital Stacy McLaughlin HT (ASCP) Lead Tech, Histology ------------------------------ Message: 5 Date: Fri, 17 Mar 2006 08:03:28 -0600 From: "Orr, Rebecca" Subject: [Histonet] double stain To: Message-ID: Content-Type: text/plain; charset="US-ASCII" Rachel, I have encountered similar issues in my journey through doublestaining. >From what I know of Triton X, it's mainly a surfactant. I'm not sure of the pH. There are solutions on the market that most likely have some type of surfactant in them, hence all the soapy bubbles in the rinsing. What solutions have you tried? What I have been successful with in this type scenario is trying a combination of either a lighter (less harsh HEIR) and then either a diluted enzyme or less time in the enzyme. Can you alter the concentration of the antibodies? One using the triton might need higher concentration, or more time. When you say sequentially, are you taking one ab all the way to the chromogen then starting the second one? I would imagine this is your protocol, but that would be good to know. Becky Orr CLA,HT(ASCP) IHC Lead Evanston Northwestern Healthcare 847-570-2771 Message: 28 Date: Fri, 17 Mar 2006 07:42:38 -0500 From: "Emerson, Rachael" Subject: [Histonet] Double stain ? To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hello. I am attempting to do a sequential double stain with 2 different antibodies. Unfortunately, one requires using Triton-X 100 for antigen retrieval and the other requires using trypsin. I've run trials using several different antigen retrieval methods and these are the only ones that work. I'm afraid that performing 2 different retrieval steps will cause the stain not to work. I would really appreciate any thoughts or suggestions. I am working with paraformaldehyde fixed mouse embryos. Thanks Rachael Emerson ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 28, Issue 27 **************************************** ------------------------------ Message: 6 Date: Fri, 17 Mar 2006 09:10:51 -0500 From: Phil McArdle Subject: Re: [Histonet] Commercial micro waves To: Doug Geddes , histonet@lists.utsouthwestern.edu Message-ID: <441AC36B.70202@ebsciences.com> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hi: Check out http://www.ebsstore.com/control/category/~category_id=C Please feel free to contact me with any questions or issues you may have. Best regards, Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. Tel: 800.992.9037 x 341 Cell: 860.597.6796 Fax: 860.653.0422 PMcardle@ebsciences.com www.ebsciences.com "ADDING BRILLIANCE TO YOUR VISION" "I hate quotations. Tell me what you know." Ralph Waldo Emerson Doug Geddes wrote: > Would anyone be will to share any information good or bad about > commercial mircro waves for antigen retrieval, vendors welcomed. > > > Doug Geddes BSc., MLT > Dept of Pathology > London Health Sciences Centre > London, Ontario, Canada > > ----------------------------------------- > This information is directed in confidence solely to the person > named above and may contain confidential and/or privileged > material. This information may not otherwise be distributed, > copied or disclosed. If you have received this e-mail in error, > please notify the sender immediately via a return e-mail and > destroy original message. Thank you for your cooperation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Fri, 17 Mar 2006 09:04:02 -0600 From: "Orr, Rebecca" Subject: [Histonet] Training Med Techs To: Cc: "Delk, Linda" Message-ID: Content-Type: text/plain; charset="US-ASCII" Hello everyone. I would appreciate any feedback from those of you who may have had to train MT's (ASCP) to work in Histology. They would be trained as histo techs with the intent to promote them into Anatomic Pathology (Histology) management positions. Candid comments welcome, especially from MT's who now work in histology! To me it would be like trying to train a policeman to be a fireman, it's a career, not a job, right? We see a HT shortage in the Chicago area, but I am unsure how to address this. Degreed individuals have proven critical thinking skills via a traditional education pathway, so I see the advantages, but to ignore very capable HT managers with proven management and organizational skills via non traditional pathways is becoming an issue with me. I mean it's not like Non degreed HT's are stooopid or something. Thank you Becky Becky Orr CLA,HT(ASCP) IHC Lead Evanston Northwestern Healthcare 847-570-2771 ------------------------------ Message: 8 Date: Fri, 17 Mar 2006 07:04:50 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Somewhat rhetorical question regarding antibody incubation time To: Jackie M O'Connor , histonet@lists.utsouthwestern.edu Message-ID: <20060317150450.43863.qmail@web61217.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 If it works it will expedite your TAT and I would certainly recommend that approach, just one thing though: you cannot equate a 2:1 increased concentration with a 16:1 reduction of incubation time + a 6:1 fold increase of temperature. You should make several tests to find out the correct new dilution factor that will allow you to change the incubation time and temperature to what you desire. Hope this will help you! Ren? J. Jackie M O'Connor wrote: If you have an antibody that the manufacturer recommends overnight incubation at 4C at 1:1200 dilution - is there anything to be gained from increasing the concentration (1:600) and incubating for one hour RT instead? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Travel Find great deals to the top 10 hottest destinations! ------------------------------ Message: 9 Date: Fri, 17 Mar 2006 10:15:43 EST From: KDwyer3322@aol.com Subject: [Histonet] New NY regulations To: histonet@lists.utsouthwestern.edu Message-ID: <22b.87e55fa.314c2c9f@aol.com> Content-Type: text/plain; charset="ISO-8859-1" Histonetters from New York: As stated below will the new regulations affect histotechnicians? Any help would be appreciated. Thanks, Kathy Dwyer The New York State Office of the Professions has posted updated information on our state licencing of clinical laboratory technologists, technicians and Cytotechnologists as pasted below the direct link. http://www.op.nysed.gov/clp-mar06update.htm March 2006 The State Education Department, with the assistance of the State Board for Clinical Laboratory Technology and interested parties are in the process of developing draft regulations that will be necessary for the licensure of the professionals as clinical laboratory technologists, Cytotechnologists, and clinical laboratory technicians. Once these draft regulations are completed, they will be submitted to all interested parties and published in the State Register for comment. At the end of this process, regulations will be presented for consideration and action by the Board of Regents. It is anticipated that this process will be completed by mid-summer in 2006. Applications packets for licensure will not be available until the regulations are enacted. Prior to the enactment of the regulations, the Department cannot provide any specific information to individuals regarding licensure under the "Special Provisions" or "grandparenting.." As soon as the regulations are enacted, this information will be available. As soon as the regulations are enacted by the State Board of Regents, the Department will publish a Guide to Practice for the professions of Clinical Laboratory Technology, Clinical Laboratory Technician, and Cytotechnology, as well as an application packet for these professions. When they are available, the applications will be published on this website and can be downloaded and submitted to the Department. Applicants who wish to receive a hard copy of the Guide to Practice and the Application Packet may submit an e-mail request for the information now, but should not expect to receive this information before the summer of 2006. A notice will be put on this website when the applications are ready to be mailed. Clinical Laboratory Technicians, Clinical Laboratory Technologists and Cytotechnologists who want to request to be placed on a mailing list now for a hard copy of the application packet and receive it in the summer of 2006 should send an e-mail to OPFORMS@mail.nysed.gov, giving their name and address, and specify the packet they want by: Code 92A and profession title: Clinical Laboratory Technologist/Clinical Laboratory Technician, or Code 93A and profession title: Cytotechnology. http://www.op.nysed.gov/clp-mar06update.htm Page last updated: 03/15/2006 12:07:36 ------------------------------ Message: 10 Date: Fri, 17 Mar 2006 07:20:07 -0800 From: "Mark Elliott" Subject: [Histonet] Region IX Education Day To: Message-ID: <441A6327020000D60000464E@mail.mrl.ubc.ca> Content-Type: text/plain; charset=US-ASCII Hi everyone For anyone wanting an excuse to visit the wonderful city of Montreal, we have the perfect reason-Region IX is hosting an Education Day on May 6th, 2006 in this vibrant city. We are having 5 speakers on a variety of topics and 13 vendors will be present showcasing their products. Registration deadline is April 14th. Please visit http://www.nshregionix.org/education.html for more information, including registration forms, speaker schedule and hotel information. Hope to see you there! Mark Elliott Region IX Education Committee Chair ------------------------------ Message: 11 Date: Fri, 17 Mar 2006 10:24:16 -0500 From: "Kellar, Eric C" Subject: [Histonet] RE: Sudan Black Staining To: histonet@lists.utsouthwestern.edu Message-ID: <6843061CE6B98E4B96590D4F299618F801583BB6@qdcws0117.us.qdx.com> Content-Type: text/plain; charset=iso-8859-1 To slow Sudan Black B (a nonionic, hydrophobic dye) 'bleeding' on unfixed cryostat sections, try running a duplicate slide and compare with this method: 1. Air dry duplicate slide after staining. 2. Clear slide through 3 changes of fresh Xylene or Xylene Substitute. 3. Glass coverslip using a permanent mounting media. The synthetic mountant will eventually dissolve some of the dye-lipid complex, but it will give you a longer window of time to microscopically examine your slides. Eric C. Kellar Histology/Immunohistochemistry Quest Diagnostics Inc. Miami Hi, I am working on the Sudan Black stain. Do the slides need to be read immediately due to bleeding of the stain after approximately 24 hours? Thanks for any information you can give me about the stain. Sharon Willman ============================================================================ == The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. ============================================================================ == ------------------------------ Message: 12 Date: Fri, 17 Mar 2006 09:27:50 -0600 From: "Orr, Rebecca" Subject: [Histonet] clarifying the MT -HT training To: Message-ID: Content-Type: text/plain; charset="US-ASCII" In clarification, I don't think I worded that right. It's a salary compensation issue. A non degreed HT is expected to take on the same responsibilities as a histology supervisor for 10K less than a trained MT crossing over to HT. So to reiterate, it's not so much being eligible to handle to management, it's about taking less salary for the same responsibility. Thank you all for the feedback this is seems like a warm issue! Becky Orr CLA,HT(ASCP) IHC Lead Evanston Northwestern Healthcare 847-570-2771 ------------------------------ Message: 13 Date: Fri, 17 Mar 2006 10:35:48 -0500 From: "Smith, Allen" Subject: RE: [Histonet] Gram's iodine mystery solved To: "John Kiernan" Cc: histonet@lists.utsouthwestern.edu Message-ID: <5D2189E74151CC42BEC02906BA8996322B919F@exchsrv01.barrynet.barry.edu> Content-Type: text/plain; charset="US-ASCII" Shockingly, labels are not always correct. I have a jar labelled "indigocarmine" whose contents is insoluble in water. I once (20 years ago) opened a brand new bottle labelled "acetic anhydride" and caught a whiff of isoamyl acetate. The contents also boiled at the boiling point of isoamyl acetate (4 C higher than acetic anhydride), and it did not dissolve in hot water. I have also borrowed a bottle of bromine from a colleague (25 years ago at another school) and found that it performed brominations fairly well, but required an elevated temperature to do them. I never did find out what the contaminant was or how it got there. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Kiernan Sent: Thursday, March 16, 2006 11:43 PM To: meint002 Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Gram's iodine mystery solved Is this the end of the matter? Who swapped your iodine for iron filings? Do you have an enemy doing horrid things with the chemicals on your shelves? -- ------------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan[AT]uwo.ca http://publish.uwo.ca/~jkiernan/ http://instruct.uwo.ca/anatomy/530/index.htm _______________________________ meint002 wrote: > Dear Histos, > > Thanks to all for your advice about making Gram's Iodine soln. > > There was something wrong about the reagent iodine I was using. I borrowed > the proper iodine from another histo lab (thanks Luann) and now all is just > fine. Dissolving it in the Potassium iodide worked like a charm. > > > Joyce Meints > Histologist > > University of Minnesota > Paul and Sheila Wellstone Muscular Dystrophy Center > MMC 206 > 420 Delaware St. SE > Minneapolis, MN 55455-0392 > > e-mail: meint002@umn.edu > lab phone: 612-626-4703 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) ------------------------------ Message: 14 Date: Fri, 17 Mar 2006 09:04:50 -0700 From: Gayle Callis Subject: An aside on viewing kidney biopsies Re: [Histonet] microscopes To: "Santana, Diane" , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20060317090020.01b46438@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed We supplied laboratories with slides that have the wells in them, commonly used for viewing larger creatures like insects, etc. These are sold by Fisher, etc The biospy was floated in PBS, and you can actually use any microscope at lowest power or stereomicroscope to look for glomeruli. This prevented crushing and flattening the biopsy, a problem we encountered far to many times when bx was examined between a slide and coverslip immediately after removal. At 10:55 AM 3/16/2006, you wrote: >I am looking at microscopes for kidney bx's. My dept will start assisting >with the bx's after the bx has been removed. I have not done this in >YEARS... then we watch the tissue, if it floated it was probably fat, if it >sank to the bottom of the container it was more than likely tissue. So I am >looking for a scope that I can see if I have tissue and some light reading >as a refresher course. Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 ------------------------------ Message: 15 Date: Fri, 17 Mar 2006 11:23:54 -0500 From: "Judith LaDuc" Subject: Re: [Histonet] New NY regulations To: , Message-ID: <441A9AB5.2A60.00B8.0@trudeauinstitute.org> Content-Type: text/plain; charset=US-ASCII Hi Kathy and NY Histotechicians/technologists, Yes, the new regulations will affect us. We are classified under the heading of clinical laboratory technicians/technologists and are subject to licensure. I would recommend signing up for the mailing list and checking into the website routinely. The following is a post from the Histonet archives that has a few links and a timeline of events. http://www.histosearch.com/histonet/Jan06/HistonetNYSLicensure.html Dr. Kathleen Doyle from NYS will be presenting an informational session at the Region 1 meeting in Saratoga Springs on Friday, April 28th. The Region 1 program (April 28th & 29th) can be accessed at http://www.nyhisto.org Judy LaDuc, BS HTL ASCP President, NYS Histotechnological Society >>> 03/17/06 10:15 am >>> Histonetters from New York: As stated below will the new regulations affect histotechnicians? Any help would be appreciated. Thanks, Kathy Dwyer The New York State Office of the Professions has posted updated information on our state licencing of clinical laboratory technologists, technicians and Cytotechnologists as pasted below the direct link. http://www.op.nysed.gov/clp- mar06update.htm March 2006 The State Education Department, with the assistance of the State Board for Clinical Laboratory Technology and interested parties are in the process of developing draft regulations that will be necessary for the licensure of the professionals as clinical laboratory technologists, Cytotechnologists, and clinical laboratory technicians. Once these draft regulations are completed, they will be submitted to all interested parties and published in the State Register for comment. At the end of this process, regulations will be presented for consideration and action by the Board of Regents. It is anticipated that this process will be completed by mid- summer in 2006. Applications packets for licensure will not be available until the regulations are enacted. Prior to the enactment of the regulations, the Department cannot provide any specific information to individuals regarding licensure under the "Special Provisions" or "grandparenting.." As soon as the regulations are enacted, this information will be available. As soon as the regulations are enacted by the State Board of Regents, the Department will publish a Guide to Practice for the professions of Clinical Laboratory Technology, Clinical Laboratory Technician, and Cytotechnology, as well as an application packet for these professions. When they are available, the applications will be published on this website and can be downloaded and submitted to the Department. Applicants who wish to receive a hard copy of the Guide to Practice and the Application Packet may submit an e- mail request for the information now, but should not expect to receive this information before the summer of 2006. A notice will be put on this website when the applications are ready to be mailed. Clinical Laboratory Technicians, Clinical Laboratory Technologists and Cytotechnologists who want to request to be placed on a mailing list now for a hard copy of the application packet and receive it in the summer of 2006 should send an e- mail to OPFORMS@mail.nysed.gov, giving their name and address, and specify the packet they want by: Code 92A and profession title: Clinical Laboratory Technologist/Clinical Laboratory Technician, or Code 93A and profession title: Cytotechnology. http://www.op.nysed.gov/clp- mar06update.htm Page last updated: 03/15/2006 12:07:36 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Fri, 17 Mar 2006 11:34:15 -0500 From: "Jacqui Detmar" Subject: [Histonet] immunohistochemistry and TUNEL background staining To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi all. I have been doing IHC and TUNELs on mouse placental tissues for the past 4 years and have been having problems with oodles of background staining during the past 6 months or so. I have tried doing avidin-biotin and streptavidin-biotin blocking and also using ABC (purchased from Vector) in high pH (TBS, pH 9.4) or high salt (TBS [NaCl = 0.5M], pH 7.6) solutions. Nothing seems to be working. The IHC using biotinylated secondary antibodies and the TUNEL is done using biotinylated dUTP. I have tried using old sections that I *know* have worked in the past and I am getting the same type of background staining as my newer blocks (i.e. the problem is likely not due to fixation (always the same...24 hours in 10% phosphate-buffered formalin), nor due to differences in paraffin embedding). So, could my xylene and ethanol solutions be a problem? We get the ethanol in-house here at the hospital and the Xylene is purchased through Fisher (X16-4). What about differences in TBS or PBS (I have tried using both to see if the problem would go away and it doesn't make a difference). Any advice would be appreciated. Regards, Jacqui Detmar, Ph.D. student Samuel Lunenfeld Research Institute Mount Sinai Hospital, Toronto, Ontario, Canada ------------------------------ Message: 17 Date: Fri, 17 Mar 2006 12:03:44 -0500 From: "John A. Kiernan" Subject: Re: [Histonet] Sudan Black Staining To: Sharon E Willman Cc: histonet@lists.utsouthwestern.edu Message-ID: <441AEBF0.58CE6C9C@uwo.ca> Content-Type: text/plain; charset=us-ascii Sudan black B shouldn't bleed. After staining, rinse in 70% alcohol until non-lipid structures are destained, wash in water, and mount in an aqueous medium. The slides are good for months - probably longer. -- ------------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan[AT]uwo.ca http://publish.uwo.ca/~jkiernan/ http://instruct.uwo.ca/anatomy/530/index.htm _______________________________ Sharon E Willman wrote: > > Hi, > I am working on the Sudan Black stain. Do the slides need to be read > immediately due to bleeding of the stain after approximately 24 hours? > > Thanks for any information you can give me about the stain. > Sharon Willman > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 28, Issue 28 **************************************** From cpgarcia <@t> salk.edu Fri Mar 17 12:40:47 2006 From: cpgarcia <@t> salk.edu (Carlos G. Perez-Garcia) Date: Fri Mar 17 12:40:18 2006 Subject: [Histonet] Re: message 16 tunel problems In-Reply-To: <200603171808.k2HI8jtN008923@emex1.salk.edu> References: <200603171808.k2HI8jtN008923@emex1.salk.edu> Message-ID: <0218D53D-FE43-45A3-A7A1-685CB248FA60@salk.edu> Hi, I suggested you to boiling samples in citrate buffer at ph 6 it can be helpful to your problems of background, i work in brain and is very useful for tunel, mostly in paraffin sections. best c Carlos G. Perez-Garcia, Ph.D. Molecular Neurobiology Lab (MNL-O) The Salk Institute 10010 North Torrey Pines Road 92037 La Jolla, CA USA cpgarcia@salk.edu Fax: 858 558 6207 On Mar 17, 2006, at 10:08 AM, histonet- request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Somewhat rhetorical question regarding antibody incubation > time (Jackie M O'Connor) > 2. Re: Cd8 on rat tissue (Susan Q Wells) > 3. Commercial micro waves (Doug Geddes) > 4. RE: Histonet Digest, Vol 28, Issue 27 (Orr, Rebecca) > 5. double stain (Orr, Rebecca) > 6. Re: Commercial micro waves (Phil McArdle) > 7. Training Med Techs (Orr, Rebecca) > 8. Re: Somewhat rhetorical question regarding antibody > incubation time (Rene J Buesa) > 9. New NY regulations (KDwyer3322@aol.com) > 10. Region IX Education Day (Mark Elliott) > 11. RE: Sudan Black Staining (Kellar, Eric C) > 12. clarifying the MT -HT training (Orr, Rebecca) > 13. RE: Gram's iodine mystery solved (Smith, Allen) > 14. An aside on viewing kidney biopsies Re: [Histonet] > microscopes (Gayle Callis) > 15. Re: New NY regulations (Judith LaDuc) > 16. immunohistochemistry and TUNEL background staining (Jacqui > Detmar) > 17. Re: Sudan Black Staining (John A. Kiernan) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 17 Mar 2006 06:51:56 -0600 > From: "Jackie M O'Connor" > Subject: [Histonet] Somewhat rhetorical question regarding antibody > incubation time > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > If you have an antibody that the manufacturer recommends overnight > incubation at 4C at 1:1200 dilution - > is there anything to be gained from increasing the concentration > (1:600) > and incubating for one hour RT instead? > > > > ------------------------------ > > Message: 2 > Date: Fri, 17 Mar 2006 07:53:19 -0500 > From: Susan Q Wells > Subject: Re: [Histonet] Cd8 on rat tissue > To: Patsy Ruegg > Cc: histonet@pathology.swmed.edu > Message-ID: <441AB13F.30805@bms.com> > Content-Type: text/plain; format=flowed; charset=us-ascii > > I have used mouse anti-rat from BD at .1ug/ml on frozen rat sections > with Dako's LSAB2 rat kit (standard protocol) with good results. > > Patsy Ruegg wrote: > >> I was wondering what is being used for CD8 labeling of rat >> tissue? I assume >> that it still has to be done on frozen tissue? I have done a lot >> of rat >> anti-cd8 on mouse tissue using BD antibody but now I need to do it >> on rat >> tissue. >> Thanks, >> Patsy >> >> Patsy Ruegg, HT(ASCP)QIHC >> IHCtech, LLC >> Fitzsimmons BioScience Park >> 12635 Montview Blvd. Suite 216 >> Aurora, CO 80010 >> P-720-859-4060 >> F-720-859-4110 >> wk email pruegg@ihctech.net >> web site www.ihctech.net >> >> >> This email is confidential and intended solely for the use of the >> Person(s) >> ('the intended recipient') to whom it was addressed. Any views or >> opinions >> presented are solely those of the author. It may contain >> information that is >> privileged & confidential within the meaning of applicable law. >> Accordingly >> any dissemination, distribution, copying, or other use of this >> message, or >> any of its contents, by any person other than the intended >> recipient may >> constitute a breach of civil or criminal law and is strictly >> prohibited. If >> you are NOT the intended recipient please contact the sender and >> dispose of >> this e-mail as soon as possible. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > > > > ------------------------------ > > Message: 3 > Date: Fri, 17 Mar 2006 08:06:53 -0500 > From: "Doug Geddes" > Subject: [Histonet] Commercial micro waves > To: > Message-ID: > Content-Type: text/plain; charset="US-ASCII" > > Would anyone be will to share any information good or bad about > commercial mircro waves for antigen retrieval, vendors welcomed. > > > Doug Geddes BSc., MLT > Dept of Pathology > London Health Sciences Centre > London, Ontario, Canada > > ----------------------------------------- > This information is directed in confidence solely to the person > named above and may contain confidential and/or privileged > material. This information may not otherwise be distributed, > copied or disclosed. If you have received this e-mail in error, > please notify the sender immediately via a return e-mail and > destroy original message. Thank you for your cooperation. > > > > > ------------------------------ > > Message: 4 > Date: Fri, 17 Mar 2006 07:32:28 -0600 > From: "Orr, Rebecca" > Subject: [Histonet] RE: Histonet Digest, Vol 28, Issue 27 > To: > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > Stacy, > I wrote a sentence describing the issues of background staining. I > then > described what detection kits we use in our lab. We use both > polymer(Dako/Biocare) and the Ventana I view on the benchmark. I > also > added instructions to determine if Endogenous Biotin is evident > (omitting primary and secondary and just adding the label and > chromogen > on one slide, just the chromogen on a second slide...) > I put something in there about following manufacturer's > recommendations > on the data sheets. I addressed protein blocks and endogenous > peroxidase > all in the same section and called it "Blocking Reagents". > I was just inspected in February and the inspectors and I scrutinized > that regulation especially since it was new. I passed so I'm thinking > what I wrote was adequate. I'll go ahead and send you the excerpt > with > my references in a separate file. Let me know if you can't receive > files from strangers. I can fax if you'd like > Have a great weekend > Becky > > Becky Orr CLA,HT(ASCP) > IHC Lead > Evanston Northwestern Healthcare > 847-570-2771 > > > - > > Message: 6 > Date: Thu, 16 Mar 2006 14:01:46 -0500 > From: "Stacy McLaughlin" > Subject: [Histonet] Endogenous Biotin and CAP regulations > To: > Message-ID: > > dickinson.org >> > > Content-Type: text/plain; charset="us-ascii" > > > Would anyone be willing to share how they address this issue, > regarding > CAP reg: ANP.22615 "If the laboratory uses an avidin-biotin > complex > (ABC) detection system (or a related system such as > streptavidin-biotin > or neutravidin-biotin), is there a policy that addresses > nonspecific > false positive staining from endogenous biotin? > We're currently using the Ventana Nexes, with biotin-streptavidin > detection system. > Are there specific vendors your recommend for these reagents? > Your answers are appreciated! > Stacy > Stacy McLaughlin HT (ASCP) > Lead Tech, Histology > Coolely Dickinson Hospital > > > > Stacy McLaughlin HT (ASCP) > Lead Tech, Histology > > > > > > > ------------------------------ > > Message: 5 > Date: Fri, 17 Mar 2006 08:03:28 -0600 > From: "Orr, Rebecca" > Subject: [Histonet] double stain > To: > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > Rachel, > I have encountered similar issues in my journey through > doublestaining. > >> From what I know of Triton X, it's mainly a surfactant. I'm not sure > of the pH. There are solutions on the market that most likely have > some > type of surfactant in them, hence all the soapy bubbles in the > rinsing. > What solutions have you tried? > > What I have been successful with in this type scenario is trying a > combination of either a lighter (less harsh HEIR) and then either a > diluted enzyme or less time in the enzyme. Can you alter the > concentration of the antibodies? One using the triton might need > higher > concentration, or more time. > When you say sequentially, are you taking one ab all the way to the > chromogen then starting the second one? I would imagine this is your > protocol, but that would be good to know. > > Becky Orr CLA,HT(ASCP) > IHC Lead > Evanston Northwestern Healthcare > 847-570-2771 > > > > > Message: 28 > Date: Fri, 17 Mar 2006 07:42:38 -0500 > From: "Emerson, Rachael" > Subject: [Histonet] Double stain ? > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > Hello. I am attempting to do a sequential double stain with 2 > different > antibodies. > Unfortunately, one requires using Triton-X 100 for antigen > retrieval and > the > other requires using trypsin. > I've run trials using several different antigen retrieval methods and > these > are the only ones that work. > I'm afraid that performing 2 different retrieval steps will cause the > stain > not to work. I would really appreciate any thoughts or suggestions. > I am working with paraformaldehyde fixed mouse embryos. > > > Thanks > Rachael Emerson > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 28, Issue 27 > **************************************** > > > > > > ------------------------------ > > Message: 6 > Date: Fri, 17 Mar 2006 09:10:51 -0500 > From: Phil McArdle > Subject: Re: [Histonet] Commercial micro waves > To: Doug Geddes , > histonet@lists.utsouthwestern.edu > Message-ID: <441AC36B.70202@ebsciences.com> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Hi: > > Check out http://www.ebsstore.com/control/category/~category_id=C > > Please feel free to contact me with any questions or issues you may > have. > > Best regards, > > Phil McArdle > Microwave Product Manager > Energy Beam Sciences, Inc. > Tel: 800.992.9037 x 341 > Cell: 860.597.6796 > Fax: 860.653.0422 > PMcardle@ebsciences.com > www.ebsciences.com > "ADDING BRILLIANCE TO YOUR VISION" > > "I hate quotations. Tell me what you know." Ralph Waldo Emerson > > > Doug Geddes wrote: >> Would anyone be will to share any information good or bad about >> commercial mircro waves for antigen retrieval, vendors welcomed. >> >> >> Doug Geddes BSc., MLT >> Dept of Pathology >> London Health Sciences Centre >> London, Ontario, Canada >> >> ----------------------------------------- >> This information is directed in confidence solely to the person >> named above and may contain confidential and/or privileged >> material. This information may not otherwise be distributed, >> copied or disclosed. If you have received this e-mail in error, >> please notify the sender immediately via a return e-mail and >> destroy original message. Thank you for your cooperation. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 7 > Date: Fri, 17 Mar 2006 09:04:02 -0600 > From: "Orr, Rebecca" > Subject: [Histonet] Training Med Techs > To: > Cc: "Delk, Linda" > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > Hello everyone. > > I would appreciate any feedback from those of you who may have had to > train MT's (ASCP) to work in Histology. > > They would be trained as histo techs with the intent to promote them > into Anatomic Pathology (Histology) management positions. > > Candid comments welcome, especially from MT's who now work in > histology! > > To me it would be like trying to train a policeman to be a fireman, > it's a career, not a job, right? > > > > We see a HT shortage in the Chicago area, but I am unsure how to > address > this. > > > > Degreed individuals have proven critical thinking skills via a > traditional education pathway, so I see the advantages, but to ignore > very capable HT managers with proven management and organizational > skills via non traditional pathways is becoming an issue with me. > > I mean it's not like Non degreed HT's are stooopid or something. > > > > Thank you > > Becky > > > > Becky Orr CLA,HT(ASCP) > > IHC Lead > > Evanston Northwestern Healthcare > > 847-570-2771 > > > > > > ------------------------------ > > Message: 8 > Date: Fri, 17 Mar 2006 07:04:50 -0800 (PST) > From: Rene J Buesa > Subject: Re: [Histonet] Somewhat rhetorical question regarding > antibody incubation time > To: Jackie M O'Connor , > histonet@lists.utsouthwestern.edu > Message-ID: <20060317150450.43863.qmail@web61217.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > If it works it will expedite your TAT and I would certainly > recommend that approach, just one thing though: you cannot equate a > 2:1 increased concentration with a 16:1 reduction of incubation > time + a 6:1 fold increase of temperature. > You should make several tests to find out the correct new > dilution factor that will allow you to change the incubation time > and temperature to what you desire. > Hope this will help you! > Ren? J. > > Jackie M O'Connor wrote: > If you have an antibody that the manufacturer recommends overnight > incubation at 4C at 1:1200 dilution - > is there anything to be gained from increasing the concentration > (1:600) > and incubating for one hour RT instead? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Yahoo! Travel > Find great deals to the top 10 hottest destinations! > > ------------------------------ > > Message: 9 > Date: Fri, 17 Mar 2006 10:15:43 EST > From: KDwyer3322@aol.com > Subject: [Histonet] New NY regulations > To: histonet@lists.utsouthwestern.edu > Message-ID: <22b.87e55fa.314c2c9f@aol.com> > Content-Type: text/plain; charset="ISO-8859-1" > > Histonetters from New York: > As stated below will the new regulations affect histotechnicians? > Any help > would be appreciated. > Thanks, > Kathy Dwyer > > > > The New York State Office of the Professions has posted updated > information > on our state licencing of clinical laboratory technologists, > technicians and > Cytotechnologists as pasted below the direct link. > > http://www.op.nysed.gov/clp-mar06update.htm > > March 2006 > The State Education Department, with the assistance of the State > Board for > Clinical Laboratory Technology and interested parties are in the > process of > developing draft regulations that will be necessary for the > licensure of the > professionals as clinical laboratory technologists, > Cytotechnologists, and clinical > laboratory technicians. Once these draft regulations are completed, > they will > be submitted to all interested parties and published in the State > Register > for comment. At the end of this process, regulations will be > presented for > consideration and action by the Board of Regents. It is anticipated > that this > process will be completed by mid-summer in 2006. Applications > packets for licensure > will not be available until the regulations are enacted. Prior to the > enactment of the regulations, the Department cannot provide any > specific information > to individuals regarding licensure under the "Special Provisions" or > "grandparenting.." As soon as the regulations are enacted, this > information will be > available. > > As soon as the regulations are enacted by the State Board of > Regents, the > Department will publish a Guide to Practice for the professions of > Clinical > Laboratory Technology, Clinical Laboratory Technician, and > Cytotechnology, as well > as an application packet for these professions. When they are > available, the > applications will be published on this website and can be > downloaded and > submitted to the Department. Applicants who wish to receive a hard > copy of the Guide > to Practice and the Application Packet may submit an e-mail request > for the > information now, but should not expect to receive this information > before the > summer of 2006. A notice will be put on this website when the > applications are > ready to be mailed. > > Clinical Laboratory Technicians, Clinical Laboratory Technologists and > Cytotechnologists who want to request to be placed on a mailing > list now for a hard > copy of the application packet and receive it in the summer of 2006 > should > send an e-mail to OPFORMS@mail.nysed.gov, giving their name and > address, and > specify the packet they want by: > > Code 92A and profession title: Clinical Laboratory Technologist/ > Clinical > Laboratory Technician, or > Code 93A and profession title: Cytotechnology. > > http://www.op.nysed.gov/clp-mar06update.htm > Page last updated: 03/15/2006 12:07:36 > > > > > > ------------------------------ > > Message: 10 > Date: Fri, 17 Mar 2006 07:20:07 -0800 > From: "Mark Elliott" > Subject: [Histonet] Region IX Education Day > To: > Message-ID: <441A6327020000D60000464E@mail.mrl.ubc.ca> > Content-Type: text/plain; charset=US-ASCII > > Hi everyone > For anyone wanting an excuse to visit the wonderful city of > Montreal, we have the perfect reason-Region IX is hosting an > Education Day on May 6th, 2006 in this vibrant city. We are having > 5 speakers on a variety of topics and 13 vendors will be present > showcasing their products. Registration deadline is April 14th. > Please visit http://www.nshregionix.org/education.html > for more information, including registration forms, speaker > schedule and hotel information. > Hope to see you there! > Mark Elliott > Region IX Education Committee Chair > > > > > ------------------------------ > > Message: 11 > Date: Fri, 17 Mar 2006 10:24:16 -0500 > From: "Kellar, Eric C" > Subject: [Histonet] RE: Sudan Black Staining > To: histonet@lists.utsouthwestern.edu > Message-ID: > <6843061CE6B98E4B96590D4F299618F801583BB6@qdcws0117.us.qdx.com> > Content-Type: text/plain; charset=iso-8859-1 > > To slow Sudan Black B (a nonionic, hydrophobic dye) 'bleeding' on > unfixed cryostat sections, try running a duplicate slide and > compare with this method: > > 1. Air dry duplicate slide after staining. > 2. Clear slide through 3 changes of fresh Xylene or Xylene Substitute. > 3. Glass coverslip using a permanent mounting media. > > The synthetic mountant will eventually dissolve some of the dye- > lipid complex, but it will give you a longer window of time to > microscopically examine your slides. > > > Eric C. Kellar > Histology/Immunohistochemistry > Quest Diagnostics Inc. Miami > > > Hi, > I am working on the Sudan Black stain. Do the slides need to be read > immediately due to bleeding of the stain after approximately 24 hours? > > Thanks for any information you can give me about the stain. > Sharon Willman > > > ====================================================================== > ======== > The contents of this message, together with any attachments, are > intended only for the use of the person(s) to which they are > addressed and may contain confidential and/or privileged > information. Further, any medical information herein is > confidential and protected by law. It is unlawful for unauthorized > persons to use, review, copy, disclose, or disseminate confidential > medical information. If you are not the intended recipient, > immediately advise the sender and delete this message and any > attachments. Any distribution, or copying of this message, or any > attachment, is prohibited. > ====================================================================== > ======== > > > > > ------------------------------ > > Message: 12 > Date: Fri, 17 Mar 2006 09:27:50 -0600 > From: "Orr, Rebecca" > Subject: [Histonet] clarifying the MT -HT training > To: > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > In clarification, > > I don't think I worded that right. It's a salary compensation > issue. A > non degreed HT is expected to take on the same responsibilities as a > histology supervisor for 10K less than a trained MT crossing over to > HT. > > So to reiterate, it's not so much being eligible to handle to > management, it's about taking less salary for the same responsibility. > > > > Thank you all for the feedback this is seems like a warm issue! > > > > > > Becky Orr CLA,HT(ASCP) > > IHC Lead > > Evanston Northwestern Healthcare > > 847-570-2771 > > > > > > ------------------------------ > > Message: 13 > Date: Fri, 17 Mar 2006 10:35:48 -0500 > From: "Smith, Allen" > Subject: RE: [Histonet] Gram's iodine mystery solved > To: "John Kiernan" > Cc: histonet@lists.utsouthwestern.edu > Message-ID: > <5D2189E74151CC42BEC02906BA8996322B919F@exchsrv01.barrynet.barry.edu> > Content-Type: text/plain; charset="US-ASCII" > > Shockingly, labels are not always correct. I have a jar labelled > "indigocarmine" whose contents is insoluble in water. > I once (20 years ago) opened a brand new bottle labelled "acetic > anhydride" and caught a whiff of isoamyl acetate. The contents > also boiled > at the boiling point of isoamyl acetate (4 C higher than acetic > anhydride), > and it did not dissolve in hot water. > I have also borrowed a bottle of bromine from a colleague (25 > years ago > at another school) and found that it performed brominations fairly > well, but > required an elevated temperature to do them. I never did find out > what the > contaminant was or how it got there. > > Allen A. Smith, Ph.D. > Professor of Anatomy > Barry University School of Graduate Medical Sciences > Podiatric Medicine and Surgery > Miami Shores, Florida 33161 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > John Kiernan > Sent: Thursday, March 16, 2006 11:43 PM > To: meint002 > Cc: histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Gram's iodine mystery solved > > Is this the end of the matter? Who swapped your > iodine for iron filings? Do you have an enemy > doing horrid things with the chemicals on your > shelves? > -- > ------------------------------- > John A. Kiernan > Department of Anatomy and Cell Biology > The University of Western Ontario > London, Canada N6A 5C1 > kiernan[AT]uwo.ca > http://publish.uwo.ca/~jkiernan/ > http://instruct.uwo.ca/anatomy/530/index.htm > _______________________________ > meint002 wrote: >> Dear Histos, >> >> Thanks to all for your advice about making Gram's Iodine soln. >> >> There was something wrong about the reagent iodine I was using. I > borrowed >> the proper iodine from another histo lab (thanks Luann) and now >> all is > just >> fine. Dissolving it in the Potassium iodide worked like a charm. >> >> >> Joyce Meints >> Histologist >> >> University of Minnesota >> Paul and Sheila Wellstone Muscular Dystrophy Center >> MMC 206 >> 420 Delaware St. SE >> Minneapolis, MN 55455-0392 >> >> e-mail: meint002@umn.edu >> lab phone: 612-626-4703 >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > The information transmitted is intended only for the person or > entity to which it is addressed and may contain confidential, and/ > or privileged material. No confidentiality or privilege is waived > or lost by any errant transmission. If you receive this message in > error, please immediately delete it and all copies of it from your > system and notify the sender. E-mail transmission cannot be > guaranteed to be secure or error-free as information could be > intercepted, corrupted, lost, destroyed, arrive late or incomplete, > or contain viruses. > Barry University - Miami Shores, FL (http://www.barry.edu) > > > > ------------------------------ > > Message: 14 > Date: Fri, 17 Mar 2006 09:04:50 -0700 > From: Gayle Callis > Subject: An aside on viewing kidney biopsies Re: [Histonet] > microscopes > To: "Santana, Diane" , > Histonet@lists.utsouthwestern.edu > Message-ID: > <6.0.0.22.1.20060317090020.01b46438@gemini.msu.montana.edu> > Content-Type: text/plain; charset="us-ascii"; format=flowed > > We supplied laboratories with slides that have the wells in them, > commonly > used for viewing larger creatures like insects, etc. These are > sold by > Fisher, etc The biospy was floated in PBS, and you can actually use > any > microscope at lowest power or stereomicroscope to look for > glomeruli. This prevented crushing and flattening the biopsy, a > problem > we encountered far to many times when bx was examined between a > slide and > coverslip immediately after removal. > > At 10:55 AM 3/16/2006, you wrote: >> I am looking at microscopes for kidney bx's. My dept will start >> assisting >> with the bx's after the bx has been removed. I have not done this in >> YEARS... then we watch the tissue, if it floated it was probably >> fat, if it >> sank to the bottom of the container it was more than likely >> tissue. So I am >> looking for a scope that I can see if I have tissue and some light >> reading >> as a refresher course. > > Gayle Callis HTL, HT, MT(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University > Bozeman MT 59717 > > > > > ------------------------------ > > Message: 15 > Date: Fri, 17 Mar 2006 11:23:54 -0500 > From: "Judith LaDuc" > Subject: Re: [Histonet] New NY regulations > To: , > Message-ID: <441A9AB5.2A60.00B8.0@trudeauinstitute.org> > Content-Type: text/plain; charset=US-ASCII > > Hi Kathy and NY Histotechicians/technologists, > > Yes, the new regulations will affect us. We are classified under the > heading of clinical laboratory technicians/technologists and are > subject > to licensure. I would recommend signing up for the mailing list and > checking into the website routinely. > > The following is a post from the Histonet archives that has a few > links > and a timeline of events. > http://www.histosearch.com/histonet/Jan06/HistonetNYSLicensure.html > Dr. Kathleen Doyle from NYS will be presenting an informational > session > at the Region 1 meeting in Saratoga Springs on Friday, April 28th. The > Region 1 program (April 28th & 29th) can be accessed at > http://www.nyhisto.org > > Judy LaDuc, BS HTL ASCP > President, NYS Histotechnological Society > >>>> 03/17/06 10:15 am >>> > Histonetters from New York: > As stated below will the new regulations affect histotechnicians? Any > help > would be appreciated. > Thanks, > Kathy Dwyer > > > > The New York State Office of the Professions has posted updated > information > on our state licencing of clinical laboratory technologists, > technicians and > Cytotechnologists as pasted below the direct link. > > http://www.op.nysed.gov/clp- mar06update.htm > > March 2006 > The State Education Department, with the assistance of the State Board > for > Clinical Laboratory Technology and interested parties are in the > process of > developing draft regulations that will be necessary for the licensure > of the > professionals as clinical laboratory technologists, Cytotechnologists, > and clinical > laboratory technicians. Once these draft regulations are completed, > they will > be submitted to all interested parties and published in the State > Register > for comment. At the end of this process, regulations will be presented > for > consideration and action by the Board of Regents. It is anticipated > that this > process will be completed by mid- summer in 2006. Applications packets > for licensure > will not be available until the regulations are enacted. Prior to the > enactment of the regulations, the Department cannot provide any > specific information > to individuals regarding licensure under the "Special Provisions" or > "grandparenting.." As soon as the regulations are enacted, this > information will be > available. > > As soon as the regulations are enacted by the State Board of Regents, > the > Department will publish a Guide to Practice for the professions of > Clinical > Laboratory Technology, Clinical Laboratory Technician, and > Cytotechnology, as well > as an application packet for these professions. When they are > available, the > applications will be published on this website and can be downloaded > and > submitted to the Department. Applicants who wish to receive a hard > copy > of the Guide > to Practice and the Application Packet may submit an e- mail request > for the > information now, but should not expect to receive this information > before the > summer of 2006. A notice will be put on this website when the > applications are > ready to be mailed. > > Clinical Laboratory Technicians, Clinical Laboratory Technologists and > > Cytotechnologists who want to request to be placed on a mailing list > now for a hard > copy of the application packet and receive it in the summer of 2006 > should > send an e- mail to OPFORMS@mail.nysed.gov, giving their name and > address, and > specify the packet they want by: > > Code 92A and profession title: Clinical Laboratory > Technologist/Clinical > Laboratory Technician, or > Code 93A and profession title: Cytotechnology. > > http://www.op.nysed.gov/clp- mar06update.htm > Page last updated: 03/15/2006 12:07:36 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 16 > Date: Fri, 17 Mar 2006 11:34:15 -0500 > From: "Jacqui Detmar" > Subject: [Histonet] immunohistochemistry and TUNEL background staining > To: > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > Hi all. I have been doing IHC and TUNELs on mouse placental > tissues for the past 4 years and have been having problems with > oodles of background staining during the past 6 months or so. I > have tried doing avidin-biotin and streptavidin-biotin blocking and > also using ABC (purchased from Vector) in high pH (TBS, pH 9.4) or > high salt (TBS [NaCl = 0.5M], pH 7.6) solutions. Nothing seems to > be working. The IHC using biotinylated secondary antibodies and > the TUNEL is done using biotinylated dUTP. I have tried using old > sections that I *know* have worked in the past and I am getting the > same type of background staining as my newer blocks (i.e. the > problem is likely not due to fixation (always the same...24 hours > in 10% phosphate-buffered formalin), nor due to differences in > paraffin embedding). > > So, could my xylene and ethanol solutions be a problem? We get the > ethanol in-house here at the hospital and the Xylene is purchased > through Fisher (X16-4). What about differences in TBS or PBS (I > have tried using both to see if the problem would go away and it > doesn't make a difference). > > Any advice would be appreciated. > > Regards, > > Jacqui Detmar, Ph.D. student > Samuel Lunenfeld Research Institute > Mount Sinai Hospital, > Toronto, Ontario, Canada > > > ------------------------------ > > Message: 17 > Date: Fri, 17 Mar 2006 12:03:44 -0500 > From: "John A. Kiernan" > Subject: Re: [Histonet] Sudan Black Staining > To: Sharon E Willman > Cc: histonet@lists.utsouthwestern.edu > Message-ID: <441AEBF0.58CE6C9C@uwo.ca> > Content-Type: text/plain; charset=us-ascii > > Sudan black B shouldn't bleed. After staining, > rinse in 70% alcohol until non-lipid structures > are destained, wash in water, and mount in an > aqueous medium. The slides are good for months - > probably longer. > -- > ------------------------------- > John A. Kiernan > Department of Anatomy and Cell Biology > The University of Western Ontario > London, Canada N6A 5C1 > kiernan[AT]uwo.ca > http://publish.uwo.ca/~jkiernan/ > http://instruct.uwo.ca/anatomy/530/index.htm > _______________________________ > Sharon E Willman wrote: >> >> Hi, >> I am working on the Sudan Black stain. Do the slides need to be read >> immediately due to bleeding of the stain after approximately 24 >> hours? >> >> Thanks for any information you can give me about the stain. >> Sharon Willman >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 28, Issue 28 > **************************************** > From TJJ <@t> Stowers-Institute.org Fri Mar 17 13:12:55 2006 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Fri Mar 17 13:13:16 2006 Subject: [Histonet] Re: Somewhat rhetorical question regarding antibody incubation time Message-ID: Dear Jackie, I think it depends on the antibody. I found this explanation on antibody equilibrium at the Chemicon website (http://www.chemicon.com/resource/ANT101/a1.asp): "The time taken to reach equilibrium is dependent on the rate of diffusion and the affinity of the antibody for the antigen, and can vary widely. The affinity constant for antibody-antigen binding can span a wide range, extending from below 105 mol-1 to above 1012 mol-1. Affinity constants can be affected by temperature, pH and solvent. Affinity constants can be determined for monoclonal antibodies, but not for polyclonal antibodies, as multiple bondings take place between polyclonal antibodies and their antigens." Some antibodies may react at room temp for one hour at 2x the standard dilution (which is used at 4 degrees C). Other times you can use the exact same concentration as you would overnight in the fridge, and your staining is as intense, but with possibly more background. If you're trying to automate the antibody by bringing it to room temp, try a variety of antibody concentrations and see if you can duplicate the results you get from the refrigerated incubation. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64133 From TJJ <@t> Stowers-Institute.org Fri Mar 17 13:19:28 2006 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Fri Mar 17 13:19:43 2006 Subject: [Histonet] Re: Double stain ? Message-ID: Rachel, Are you using chromogenic or fluorescent visualization? If chromogenic, then do a sequential double stain using the Triton treatment and antibody complex first, use a stable chromogen (like DAB), and then do your enzyme digestion and second antibody complex. If fluorescent, that makes things a lot trickier. Sometimes we just can't do fluorescent doublestaining because the pretreatments are incompatible. The answer then is to use serial sections and you can often do single staining on each slide, and then compare the same area in adjacent sections. Not the best, but it's certainly a reasonable alternative if the chromogenic techniques with DAB aren't suitable (as with expected co-expression). Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64133 From japoteete <@t> saintfrancis.com Fri Mar 17 14:05:23 2006 From: japoteete <@t> saintfrancis.com (Poteete, Jacquie A.) Date: Fri Mar 17 14:06:01 2006 Subject: [Histonet] Training Med Techs Message-ID: Well, I guess I'll stick my neck out, since I haven't seen many replies. I'm an MT(ASCP)QIHC working as IHC Lead Technologist. When I interviewed for this position, there were evidently no acceptable candidates from Histology. I believe that I got the job because I'm an older tech who went to Medical Technology School right after Columbus discovered America, and we were required to complete a Histology rotation back then. It's a shame it isn't still required. I certainly don't believe I'm any better qualified than a Histologist, but I was in the right place at the right time. Actually, I will always be grateful to Patti Loykasek who moved to Phenopath, for providing the opening for the position, and for having the skill to train me in the bare basics of the job in 5 days. It makes no sense to me why a qualifed histotech would be passed over in favor of a medical technologist, because both are perfectly capable of learning management, organizational, or technical skills. A degree does not necessarily make anyone a good medical technologist (even though it is required) or a good histologist, but many institutions want those initials behind your name. Jacquie Poteete MT(ASCP)QIHC Lead Technologist, IHC Laboratory Saint Francis Hospital, Tulsa, Oklahoma japoteete@saintfrancis.com -----Original Message----- From: Orr, Rebecca [mailto:ROrr@enh.org] Sent: Friday, March 17, 2006 9:04 AM To: histonet@lists.utsouthwestern.edu Cc: Delk, Linda Subject: [Histonet] Training Med Techs Hello everyone. I would appreciate any feedback from those of you who may have had to train MT's (ASCP) to work in Histology. They would be trained as histo techs with the intent to promote them into Anatomic Pathology (Histology) management positions. Candid comments welcome, especially from MT's who now work in histology! To me it would be like trying to train a policeman to be a fireman, it's a career, not a job, right? We see a HT shortage in the Chicago area, but I am unsure how to address this. Degreed individuals have proven critical thinking skills via a traditional education pathway, so I see the advantages, but to ignore very capable HT managers with proven management and organizational skills via non traditional pathways is becoming an issue with me. I mean it's not like Non degreed HT's are stooopid or something. Thank you Becky Becky Orr CLA,HT(ASCP) IHC Lead Evanston Northwestern Healthcare 847-570-2771 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********* Email Confidentiality Statement ********* Visit http://www.saintfrancis.com/emailconf.asp From JMacDonald <@t> mtsac.edu Fri Mar 17 14:20:22 2006 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Fri Mar 17 14:20:36 2006 Subject: [Histonet] Training Med Techs Message-ID: Becky, I am a Med Tech who works in Histology. Th receive is a definite asset in the Histology fiel are in immunology, chemistry, hematology and micro which overlap into Histo. That said, I do not think t should receive the management positions over certified Histot The position should go to the person best suited for the job, a many times it is the Histotech that is better qualified. I do beli eve though, that the Histotech should be certified. Jennifer MacD -----histonet-bounces@lists.utsouthwes To: From: "Orr, Sent by: histonet-bounces@lists.utsouthwe Date: 03/17/2006 07:04AM cc: "Delk, Linda" jhmi.edu Fri Mar 17 14:56:21 2006 From: igor.nasonkin <@t> jhmi.edu (IGOR NASONKIN) Date: Fri Mar 17 14:56:29 2006 Subject: [Histonet] pls recommend reliable antibody: cytoplasmic, neuronal, not human Message-ID: Dear histonetters, Can you recommend a reliable antibody which stains cytoplasm of neurons, works in rat but does not crossreact with human cells. will need it for frozen sections. Thank you! Dr. Igor O. Nasonkin Ph.D. Postdoctoral Fellow Pathology/Neuropathology Johns Hopkins University Ross Research Bldg Rm 563 720 Rutland Ave Baltimore MD 21205 tel: 617-388-4104 Igor.Nasonkin@jhmi.edu From bjdewe <@t> aol.com Fri Mar 17 15:08:39 2006 From: bjdewe <@t> aol.com (bjdewe@aol.com) Date: Fri Mar 17 15:08:50 2006 Subject: [Histonet] CD31 Message-ID: <8C8181E19022BA8-1C00-953@FWM-D36.sysops.aol.com> I know it's probably in the archives but can anyone give me the name and manufacturer of a CD31 antibody that work well on rat tissue? Cheers, Lorie ******************************************************************* Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s)and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ******************************************************************* From froyer <@t> bitstream.net Fri Mar 17 15:25:01 2006 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Mar 17 15:25:13 2006 Subject: [Histonet] Training Med Techs In-Reply-To: Message-ID: <001d01c64a09$40f113e0$6f01a80a@fords> Becky, The short answer is... This can not be done. Med Techs can not be trained! Do not try this at home!! Seriously though... I am no longer a practicing Laboratory Scientist (nee Med. Tech. (ASCP)), but when I was I did supervise a Histology Lab at one time. My situation was that, like Jacquie, I went through the program back when Adam was just a little boy. In our year of residency, we were required to have 8 weeks rotation through Histology. There were approximately 30 Histology questions on our M.T. Registry exam. (and they were hard too!) So I had both practical and didactic exposure to the world of Histology. I deeply regret that current M.Ts. are not given this same opportunity. Near the end of my M.T. career, the lab that I was associated with had 30 M.Ts. (24 hr. shift), 3 H.Ts., and one Cytologist. Every once in awhile, due to vacations and sick leave, they would have no one to cover Histology. I and one other MT were recruited to cover in these times of need. When the supervisory Histo Tech retired, they hired a recent HT graduate as a replacement. The Supervisory position was offered to all three H.Ts., but they all turned it down. None of them wanted to be manager, though the two older ones were more than qualified. Long story short, I was offered the position, and I took it. (I also had to split my time with the Special Chemistry Dept., but that's another story). This is not, as you say, trying to "train a policeman to be a fireman". Anatomical and Clinical Laboratory Science are compatible in a basic sense. I do agree that a qualified Histo Tech would be first choice for a management position in a Histology Lab. I feel from your message, however, that this is not the real concern. I would have to ask... are sure that a future placement as a Histology manager the true reason you are being asked to train M.Ts. in histology? Or is this an assumption? If it is not, have you made your interest known to the Administrative Director (Head Pathologist?), that you would be qualified for a management position and that you wish to be considered? Is this an issue of having a 4-year college degree? Do you have a degree? Is it required for a management position at your hospital? If it is not, you (or one of you H.T. colleges) should make it know that you are the better person for the job. You could turn out looking like a hero because you saved that lab all that money in training a M.T. when there is a H.T. already trained who can do the job. Go for it!! ~ Ford Ford M. Royer, B.Sc., MT(ASCP) Sales Manager, Histology Product Division Minnesota Medical Specialists, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 763-542-8725 phone 888-790-9686 toll free 763-546-4830 fax email web -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Poteete, Jacquie A. Sent: Friday, March 17, 2006 2:05 PM To: 'Orr, Rebecca'; histonet@lists.utsouthwestern.edu Cc: Delk, Linda Subject: RE: [Histonet] Training Med Techs Well, I guess I'll stick my neck out, since I haven't seen many replies. I'm an MT(ASCP)QIHC working as IHC Lead Technologist. When I interviewed for this position, there were evidently no acceptable candidates from Histology. I believe that I got the job because I'm an older tech who went to Medical Technology School right after Columbus discovered America, and we were required to complete a Histology rotation back then. It's a shame it isn't still required. I certainly don't believe I'm any better qualified than a Histologist, but I was in the right place at the right time. Actually, I will always be grateful to Patti Loykasek who moved to Phenopath, for providing the opening for the position, and for having the skill to train me in the bare basics of the job in 5 days. It makes no sense to me why a qualifed histotech would be passed over in favor of a medical technologist, because both are perfectly capable of learning management, organizational, or technical skills. A degree does not necessarily make anyone a good medical technologist (even though it is required) or a good histologist, but many institutions want those initials behind your name. Jacquie Poteete MT(ASCP)QIHC Lead Technologist, IHC Laboratory Saint Francis Hospital, Tulsa, Oklahoma japoteete@saintfrancis.com -----Original Message----- From: Orr, Rebecca [mailto:ROrr@enh.org] Sent: Friday, March 17, 2006 9:04 AM To: histonet@lists.utsouthwestern.edu Cc: Delk, Linda Subject: [Histonet] Training Med Techs Hello everyone. I would appreciate any feedback from those of you who may have had to train MT's (ASCP) to work in Histology. They would be trained as histo techs with the intent to promote them into Anatomic Pathology (Histology) management positions. Candid comments welcome, especially from MT's who now work in histology! To me it would be like trying to train a policeman to be a fireman, it's a career, not a job, right? We see a HT shortage in the Chicago area, but I am unsure how to address this. Degreed individuals have proven critical thinking skills via a traditional education pathway, so I see the advantages, but to ignore very capable HT managers with proven management and organizational skills via non traditional pathways is becoming an issue with me. I mean it's not like Non degreed HT's are stooopid or something. Thank you Becky Becky Orr CLA,HT(ASCP) IHC Lead Evanston Northwestern Healthcare 847-570-2771 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********* Email Confidentiality Statement ********* Visit http://www.saintfrancis.com/emailconf.asp _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From clcses <@t> gmail.com Fri Mar 17 15:37:47 2006 From: clcses <@t> gmail.com (Carmen Contreras-Sesvold) Date: Fri Mar 17 15:37:54 2006 Subject: [Histonet] sudan black information "the cliff notes" Message-ID: <46a3be380603171337o1d617eb2r9495e38a1681924b@mail.gmail.com> {Here are the "histonet" notes I saved regarding the use of sudan black B, like I said, I have a lot to be thankful for} By John Kiernan: Entering autofluorescence as a search term at http://www.histosearch.com brought up 100 hits all dated in the last 3 years. This is a problem many people have, and the number and variety of remedies indicate that there's no perfect solution. In one recent comparative study, Baschong, Suetterlin and Laeng (J. Histochem. Cytochem. 49:1565-1571, 2001) found that staining for 30 min with 0.1% sudan black B in 70% alcohol, after immunofluorescent staining, solved most autofluorescence problems. After staining the dye was washed off with a jet of a modified Hanks's solution and then immersed in the same solution for 10 minutes. The authors said this washing was necessary to avoid formation of a black precipitate. By Gayle Callis: (In response to autofluorescent in red and green channels) Sounds like you might be seeing lipofuschin granules, common autofluorescent intracellular structures in brain. To reduce autofluroescence see Baschong W, Suetterlin R, Laeng RH. Control of autofluorescence of archival formaldehyde-fixed, paraffin-embedded tissue in confocal laser scanning microscopy (CLSM). J Histochem Cytochem. 2001 Dec;49(12):1565-72. {[PMID: 11724904} They describe studies using ammonia-ethanol, borohydride, and sudan Black B alone or in combination, with a variety of fixation and section prep methods compatible with immunolabeling. You can find other literature regarding the application of these reagents to autofluorescence problems. Expect some trial-and-error to figure out what works best for your particular specimens. {I have read it and found it helpful} By Paul M.: Autofluorescence in CNS tissue, as well as some other tissues, is often due to the presence of lipofuscin. Staining with Sudan Black B after immunolabeling is often effective in suppressing such background autofluorescence. Make up 0.l% solution in 70% ethanol. Heat to boiling, then cool and filter. Optimum staining time may vary somewhat from one tissue to another and for sections of different thickness, but 10 minutes usually provides an acceptable level of suppression, and often complete suppression of autofluorescence. Quickly rinse off the stain with water or buffer, several rapid changes, and coverslip as usual. By me: I have stored the above solution for a month now at RT. And have used it on 10-20 micron thick sections and have optimal reduction of green autofluorescence in about 5 minutes of exposure. I rinse my slides in a Copeland jar filled with PBS on a platform rocker for 3 minutes and coverslip with prolong as usual. From wood <@t> dcpah.msu.edu Fri Mar 17 16:55:08 2006 From: wood <@t> dcpah.msu.edu (Thomas Wood) Date: Fri Mar 17 16:58:00 2006 Subject: [Histonet] CD 31 sheep Message-ID: Does anyone have a working Antibody and protocol for CD 31 in Sheep? -Tom Wood Diagnostic Center for Population and Animal Health Michigan State University Wood@dcpah.msu.edu From kfrese <@t> mail.med.upenn.edu Fri Mar 17 17:03:31 2006 From: kfrese <@t> mail.med.upenn.edu (Kris Frese) Date: Fri Mar 17 17:03:37 2006 Subject: [Histonet] RNALater and cryosectioning Message-ID: <441B4043.30402@mail.med.upenn.edu> Dear Histonetters, I have been trying to section RNALater-treated murine pancreas for a month now and failing miserably. I either get excessive tearing or the tissue fails to cut entirely. Upon searching, I noticed that several years ago it was mentioned that sectioning RNALater-treated tissue is impossible. Has anyone had success with this or is it now general knowledge that it cannot be done? If you were successful, what did you do? Thanks in advance for any help. Kris -- Postdoctoral Researcher Tuveson lab University of Pennsylvania Abramson Family Cancer Research Institute 421 Curie Blvd. 520 BRBII/III Philadelphia, PA 19104 Tel: (215)746-7781 Fax: (215)573-2486 Email: kfrese@mail.med.upenn.edu From pathrm35 <@t> adelphia.net Fri Mar 17 19:40:20 2006 From: pathrm35 <@t> adelphia.net (Ron Martin) Date: Fri Mar 17 19:40:28 2006 Subject: [Histonet] seeking histo position Message-ID: <000f01c64a2c$ec666db0$27233418@Pathrm35> Slightly used and abused histotech looking for a good home. Prefer private lab, second or third shift in southeastern US. May consider contract or temp position. Please contact me for resume. Thanks, Ron Martin, BS, HT (ASCP) HTL, QIHC From mbmphoto <@t> gmail.com Sat Mar 18 11:14:47 2006 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Sat Mar 18 11:14:19 2006 Subject: [Histonet] need hrGFP (humanized Renilla Green Fluorescent Protein) protocol Message-ID: If you are working using either the monoclonal or polyclonal hrGFP (humanized Renilla Green Fluorescent Protein) antibody for IHC, I hope you'd be willing to share your protocol. Please contact me. Maria Bartola Mejia University of California San Francisco Department of Neurological Surgery San Francisco, CA 94103 From marjoh3 <@t> telus.net Sat Mar 18 18:17:58 2006 From: marjoh3 <@t> telus.net (Marilyn Johnson) Date: Sat Mar 18 18:18:01 2006 Subject: [Histonet] CD4 & CD8 by Immunohistochemistry Message-ID: <004401c64aea$93c4d1b0$6501a8c0@VALUED20606295> Hi Histonetters, In approximately two months, our Lab. will be initiating a new project using skin sections of mice. We will be using IHC to demonstrate infiltrates at vaccine sites including CD4, CD8, B-cells, monocytes, macrophages, etc. Any assistance would be greatly appreciated. Thank you in advance. Marilyn Johnson Alberta Agriculture Edmonton, Alberta, Canada From franzef <@t> gmx.de Sun Mar 19 02:18:20 2006 From: franzef <@t> gmx.de (=?ISO-8859-1?Q?Franz-Josef_M=FCller?=) Date: Sun Mar 19 02:18:31 2006 Subject: [Histonet] lectin stains and antigen retrival Message-ID: Dear Histonetters, does anybody know if lectin stains are stable after the tissue section (20u, paraffin embedded) was subjected to heat antigen retrieval in citric acid pH 6.0 ? Thanks for your help in advance! Cheers franzef Dr. med. Franz-Josef M?ller Zentrum f?r Integrative Psychiatrie, Kiel Zellbiologisches Labor Niemannsweg 147 24105 Kiel GERMANY Tel.: +49 431 597 4169 From rjbuesa <@t> yahoo.com Sun Mar 19 09:35:56 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Mar 19 09:36:01 2006 Subject: [Histonet] lectin stains and antigen retrival In-Reply-To: Message-ID: <20060319153557.93612.qmail@web61214.mail.yahoo.com> Franz-Josef: Lectins, as you know, will react with carbohydrates in the tissue. The lectin will attach to the carbohydrates, and the anti-lectin-antibody will bind to the lectin identifying the carbohydrates sites in a 2 steps (indirect) reaction. Since carhohydrates are not affected with formalin fixation as proteins do, it is not necessary to subject the sections to antigen retrieval (HIER) this being a step you can avoid if just trying to use the lectins. They should be stable after HIER since some carbohydrate detecting techniques (like PAS) are done in acid pH solutions. Hope this will help! Ren? J. Franz-Josef M?ller wrote: Dear Histonetters, does anybody know if lectin stains are stable after the tissue section (20u, paraffin embedded) was subjected to heat antigen retrieval in citric acid pH 6.0 ? Thanks for your help in advance! Cheers franzef Dr. med. Franz-Josef M?ller Zentrum f?r Integrative Psychiatrie, Kiel Zellbiologisches Labor Niemannsweg 147 24105 Kiel GERMANY Tel.: +49 431 597 4169 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Mail Bring photos to life! New PhotoMail makes sharing a breeze. From jerry.santiago <@t> jax.ufl.edu Sun Mar 19 10:23:43 2006 From: jerry.santiago <@t> jax.ufl.edu (Santiago, Jerry) Date: Sun Mar 19 10:23:50 2006 Subject: [Histonet] Florida Society For Histotechnology Annual Meeting Message-ID: The Florida Society for Histotechnology is preparing for their Annual Symposium May 5 - 7, 2006 Embassy Suites Deerfield Beach Florida. All Information regarding registration, hotel and exhibiton can be accessed through our website at www.fshgroup.org. Online registration is now available.We will be offering 16 workshops and the exhibit will consist of approximately 40 exhibitors. Exhibitors can download an exhibiton form from the website and mail to the address printed. Remember this is a licensure renewal year and all your mandatory's will be offered during this symposium. Any questions please feel free to contact Susan Clark at 954-987-2020 X 5371 From pruegg <@t> ihctech.net Sun Mar 19 11:33:42 2006 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Sun Mar 19 11:33:56 2006 Subject: [Histonet] cd8 on rats Message-ID: <200603191733.k2JHXh46094702@pro12.abac.com> OK, I think I have some good advise on doing CD8 on frozen rat tissue, now has anyone gotten CD8 to work of ffpe rat tissue yet. I know, I know, this question comes up all the time for mice and I have been given procedures that I struggled over with no success, but I was hoping it might be more promising for rats. Patsy BTW Those signed up to the IHCRG list serve. We are switching host servers so be patient while we get back up to speed. Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net website www.ihctech.net From Kdrummey <@t> msn.com Sun Mar 19 18:20:21 2006 From: Kdrummey <@t> msn.com (Karen Drummey) Date: Sun Mar 19 18:20:32 2006 Subject: [Histonet] Re: Recent changes in HT Certification Message-ID: Evening: I am curious if anyone out there is aware of the reasoning behind the discontinuation of the ASCP certification for HT's as of December 2006? I was told, upon graduation, that there was a five year window within which to become registered. I graduated many years ago with a B.A., M.A.(eq) but returned to study for the AAS in Applied Lab Science and graduated with a 4.0 in Histotechnology in May 2005. I took the computerized test immediately and passed with a very high score. So many histotechs are needed; however, most of the hiring managers (many of whom I hope read this E-mail) base everyone's skill level on their current education ONLY and the lack of an ASCP certification. There is no established program in the Continental United States that culminates in the acquisition of both the HT (or HTL) degree and the ASCP certification. I welcome being corrected if someone knows of such a program. It took me several months to gain employment and I am limited in time and acquisition of tissue at this point. I was not too concerned because I believed that I had ample time to become registered. I received a letter last week from the Board of Registry directing me to sign up immediately for the November practical because the ASCP would no longer be available to HT graduates after December 2006. My main question is ....What are the implications of this move? What good is an ASCP certification if it is obsolete one month after grading? My academic advisor was not made aware of this move until I wrote and called. The program coordinators are not being informed by the Board of Registry and it is simply not just for people to be sucked into a program, allowed to pay for an education only to graduate without the ability to get the certification they will be asked for just to be considered for a hospital or lab based position. I have found the whole experience frustrating and in a constant state of flux. The HT's and HTL's with years of experience believe that all newcomers are academicians who cannot perform the tasks of the lab. The problem that I have seen is that there are no real "teachers" or instructors left in the world of Histotechnology. Everyone is an administrator and a student must force themselves into the program and into the mix of the lab in order to learn anything. I was "tested" by a HT veteran of 14 years who had to use Carson's textbook just to denote the color of collagen in a Trichrome stain. She then told my advisor that I passed the test and could even "pronounce all the big words without looking at the book!" Is this the REAL world of "old" Histology. Funny, I graduated with my first degree in the late '80's and I never met a researcher in Biological Sciences who required a textbook to test my knowledge. I have written to the Board of Registry and, of course, heard nothing. This is a sweeping and unjust move on a recent graduate and, even given the abilities, one may not be able to live, breathe and eat Histotechnology in order to meet a goal imposed at the eleventh hour. If anyone knows anything about this ruling and why these changes are being introduced so swiftly, please respond to KDrummey@msncom. If anyone can justify the ruling I would be most happy to hear from you and, if you are a Board Member, maybe you could reimburse my tuitions and expenses for the last two years I have spent proving that I remember what I learned 20 years ago and then justify the end of career that never got off the ground. Thank You...KDrummey@msn.com From Barry.R.Rittman <@t> uth.tmc.edu Sun Mar 19 22:32:07 2006 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Sun Mar 19 22:35:03 2006 Subject: [Histonet] lectin stains and antigen retrival Message-ID: I should add to the discusion that carbohydrates may be attached to proteins or lipids and those attached to lipids as in glycolipids may be removed especially if xylene is used in processing. This will affect the lectin binding for some lectins. Personal observation. Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Sun 3/19/2006 9:35 AM To: Franz-Josef "M?ller; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] lectin stains and antigen retrival Franz-Josef: Lectins, as you know, will react with carbohydrates in the tissue. The lectin will attach to the carbohydrates, and the anti-lectin-antibody will bind to the lectin identifying the carbohydrates sites in a 2 steps (indirect) reaction. Since carhohydrates are not affected with formalin fixation as proteins do, it is not necessary to subject the sections to antigen retrieval (HIER) this being a step you can avoid if just trying to use the lectins. They should be stable after HIER since some carbohydrate detecting techniques (like PAS) are done in acid pH solutions. Hope this will help! Ren? J. Franz-Josef M?ller wrote: Dear Histonetters, does anybody know if lectin stains are stable after the tissue section (20u, paraffin embedded) was subjected to heat antigen retrieval in citric acid pH 6.0 ? Thanks for your help in advance! Cheers franzef Dr. med. Franz-Josef M?ller Zentrum f?r Integrative Psychiatrie, Kiel Zellbiologisches Labor Niemannsweg 147 24105 Kiel GERMANY Tel.: +49 431 597 4169 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Mail Bring photos to life! New PhotoMail makes sharing a breeze. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Mon Mar 20 04:04:19 2006 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Mon Mar 20 04:05:26 2006 Subject: [Histonet] Re: Recent changes in HT Certification In-Reply-To: Message-ID: <000c01c64c05$a7cb64f0$cd3ed445@HPPav2> Karen - First of all - congratulations on passing the written/computer portion of the exam. This is the hardest part for candidates to pass. Most people, if they pass the written, also pass the practical. So the hardest part is done for you. Now onto the practical part, which you needed to do anyway to become a certified histotech. It just looks like this will happen a little sooner than you had planned. I don't know if you saw my very long Histonet discussion on 03/14/2006, so I'm including it below, after my comments. Program Directors of NAACLS accredited HT/HTL programs were notified about 2 months ago via email, that the PRACTICAL portion of the HT and HTL exams was being dropped - that 2006 would be the last year it was required. In the future, just the written (computer) portion of the HT and HTL exam will be required to be a certified HT/HTL. Just like MT/MLT/CT only have to take their written portions to become certified - they don't have to take a practical exam. So the HT certification is NOT being discontinued. Just the practical requirement is being discontinued. The written/computer portion only will be given in the future. (Also, a little aside - as of January 2005, the window of taking and passing both parts (written and practical) of the HT/HTL exams was changed from 5 years to 3 years. Candidates who passed the written in 2005 (like yourself) now had 3 years in which to take and pass the practical exam. That should have been in the letter all 2005 candidates received, and program directors were notified of this change last year, also.) Please note - it was program directors of NAACLS accredited HT/HTL programs that were notified of the 3 year change and the practical change, NOT academic advisors at colleges. I don't know, maybe this is one and the same person for you. Maybe it's two different people. Concerning your statement that there is no established program in the US that culminates in the HT degree and the ASCP certification. That is correct. The ONLY way to become ASCP certified is to take and pass the ASCP Board of Registry (BOR) certification exam. It's the same way with nurses - they complete nursing school and then they take their Boards separately. There is no nursing school that automatically says "you are a Board certified nurse" immediately upon graduation. In order to take the HT/HTL exam, candidates must either: - complete a NAACLS accredited histotechnology program, OR - earn their college degree with the correct amount of biology and chemistry AND obtain 1 year full time histotechnology experience. What attending a NAACLS school does is allow someone to take the ASCP BOR certification exam IMMEDIATELY upon graduation from the NAACLS program, vs. having to earn an associate degree and then obtain 1 year full time histology experience (on-the-job experience (OJT)) before being allowed to take the HT exam (or having to earn a bachelor's degree and then obtain 1 year full time histology experience before being allowed to take the HTL exam). So attending a NAACLS accredited program shortens this time frame to take the certification exam by 1 year. Also, the majority of NAACLS graduates pass the HT/HTL exams, while the majority of OJT graduates do not pass. The passing score that you have earned on the written is NOT "obsolete one month after grading". That written/computer score is still good, but the practical portion still needs to be taken and passed before becoming a certified histotech. Passing only one portion is not enough to be considered "certified". Since the practical requirement is being dropped after 2006, all candidates who previously passed the written portion must now pass the practical by the end of the 2006, in order to be ASCP certified. Candidates have between now and the deadline for the November practical grading to cut and stain nine (9) slides. If candidates who previously passed the written don't take the practical in 2006, or if they fail the 2006 practical, they still can retake the written portion of the NEW HT exam in 2007 and pass that way. So there are still options out there for these candidates. Hopefully the lab where you are working has enough tissue variety for you to obtain the tissues for the practical portion. If you need any help obtaining some of the tissues that are required, let the Histonetters know. We've helped other people obtain the one or two "hard to get" tissues. As my comments from last week (see below) state, call Gerry Piscorski at ASCP BOR for more information about why the practical was dropped, or to talk about your concerns about your status. She is definitely willing to talk with people. This is definitely a big change for the histotech community. As for your concerns about the quality of your training, please call NAACLS (773-714-8880). They are the agency that accredits HT/HTL programs (as well as MT/MLT/etc.). They need to hear your concerns and problems with the rotation part of your program. Comments from last week below. Peggy A. Wenk, HTL(ASCP)SLS Program Director, HT and HTL Programs William Beaumont Hospital Royal Oak, MI 48073 - - - COMMENTS FROM LAST WEEK: Yes, the HT and HTL practical exams are being discontinued starting in 2007. In other words, 2006 is the last year it will be required. (If you're not interested, please hit "delete" at this point, as I'm going to get wordy.) There was information on the ASCP Board of Registry (BOR) web page. However, ASCP recently redid their entire web page, and this information is in queue to be put back up on the new pages. (In other words, they didn't drop it, it just hasn't been put back up.) I'll fill you in on what I know, learned by reading an email sent to all HT/HTL program directors about 2 months ago, and what was talked about at educator's forum at the 2005 NSH meeting in Florida. Also, I talked with Dr. Blair Holladay (head of ASCP BOR) when the email first came out, and with Gerri Piscorski (exam manager) (312-541-4887), after that, to get more information. Histotechs were the only category still required to do a practical. Med techs don't have to prove they can make a blood smear or can do a Gram stain or can streak an agar plate. Cytotechs don't have to prove they can make a FNA smear or do a Pap stain. Phlebotomists don't have to prove they can get blood from someone with collapsed veins, or do a heel stick on a newborn. All other categories are tested by just a written exam. The majority of histotechs pass the practical portion. Of those that don't pass the practical, the majority of these also did not pass the written portion. In other words, very few people pass the written but fail the practical. Most either pass the practical/fail the written, or they fail both parts, or they pass both parts. So the written portion of the HT/HTL exam was a better indicator of who would pass/fail both parts, than the practical exam. The cost of the grading of the practical exams was very high - flying histotechs and pathologists into Chicago from all over the country, putting them up in hotels for the weekend, feeding them. The graders were not being extravagent - pizza for lunch, sharing cabs to the grading center, etc. The number of slides was reduced from 15 to 9, in part to reduce the number of graders needed, thus reducing the cost. (By statistically picking the right combination of tissues and stains, 9 slides were giving the same pass/fail rate as the previous 15 slides.) The fee charged to the HT/HTL candidates did not cover the cost of grading the practical exam. Not even when an additional $75 fee was added. So ASCP BOR was losing money with each practical exam. Due to concerns about HIPAA, confidentiality, shipping, etc., many candidates were having difficult times obtaining tissue (especially students in college based histotech programs, that were doing rotations in hospital labs for, say, 2 months). This idea of dropping the practical exam has been around for quite a few years. Reducing the number of slides on the practical from 15 to 9 was a compromise, a stepping stone if you would. The people on the ASCP BOR Histotechnology Exam Committee have been taking pictures of poor staining and sectioning artifacts. These will be incorporated into future written exams as troubleshooting and problem-solving questions. What are the causes and how to correct thick/thin sections, wrinkles, folds, splits, microchatter, etc. So the exam is being re-written to incorporate more staining and sectioning problems. Remember, the ASCP BOR Histology Exam Committee with histotechs very involved with NSH, were involved in this discussion/decision. NSH Board has a representative on the Histology Exam Committee/ASCP Board of Governors, who was involved with this topic. ASCP BOR also talked with program directors of HT/HTL schools about this at the NSH convention, asking their opinion. And this topic has been discussed at various committees that I've been on, for the past several years. So it's been coming, slowly, for somewhere over the last 6 years, maybe closer to 10 years, that I'm aware of. Now, some questions you may be having - What is going to happen to the people who didn't pass the practical last year or the year before (2005, 2004)? Each one has been sent a letter, informing them at they must take and pass the practical in 2006 (this year). If they don't pass the practical this year, then they will not have passed their HT or HTL exam. - What about the people taking the HT/HTL exam for the first time this year (2006), who don't pass? They will be given one more year (2007) to take the "make up" practical, in order to pass their HT/HTL exam. So, for just these few people, they will be allowed to take the practical in 2007. But no one else. - How will I know if someone I'm thinking about hiring can really cut or stain, if they don't have to do a practical exam for ASCP? My suggestion - having them do a "practical exam" as part of their interview with you. Hand them 6 blocks, put them in front of a microtome, and give them 20 minutes to section. Then interview them more (to use up time during the drying), and have them load the H&E stainer. While the slides are staining, have a folder of slides for them to look at - some H&E, some special stains. Ask them to identify the stains or the tissue or what's wrong with a poorly stained slide. As long as you use the same type of tissues in the blocks, and the same stained slides, and have EVERY candidate do the test - this is perfectly acceptable way to assess someone you are interviewing for a position. By this time, the H&Es are done. Have them coverslip them. If you still have questions, PLEASE contact Gerry (see direct phone number above). She is very willing to talk with people about this (or any other question you have about the HT/HTL exams. ASCP is THE best place to go to for questions about ASCP. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Karen Drummey Sent: Sunday, March 19, 2006 7:20 PM To: histonet Subject: [Histonet] Re: Recent changes in HT Certification Evening: I am curious if anyone out there is aware of the reasoning behind the discontinuation of the ASCP certification for HT's as of December 2006? I was told, upon graduation, that there was a five year window within which to become registered. I graduated many years ago with a B.A., M.A.(eq) but returned to study for the AAS in Applied Lab Science and graduated with a 4.0 in Histotechnology in May 2005. I took the computerized test immediately and passed with a very high score. So many histotechs are needed; however, most of the hiring managers (many of whom I hope read this E-mail) base everyone's skill level on their current education ONLY and the lack of an ASCP certification. There is no established program in the Continental United States that culminates in the acquisition of both the HT (or HTL) degree and the ASCP certification. I welcome being corrected if someone knows of such a program. It took me several months to gain employment and I am limited in time and acquisition of tissue at this point. I was not too concerned because I believed that I had ample time to become registered. I received a letter last week from the Board of Registry directing me to sign up immediately for the November practical because the ASCP would no longer be available to HT graduates after December 2006. My main question is ....What are the implications of this move? What good is an ASCP certification if it is obsolete one month after grading? My academic advisor was not made aware of this move until I wrote and called. The program coordinators are not being informed by the Board of Registry and it is simply not just for people to be sucked into a program, allowed to pay for an education only to graduate without the ability to get the certification they will be asked for just to be considered for a hospital or lab based position. I have found the whole experience frustrating and in a constant state of flux. The HT's and HTL's with years of experience believe that all newcomers are academicians who cannot perform the tasks of the lab. The problem that I have seen is that there are no real "teachers" or instructors left in the world of Histotechnology. Everyone is an administrator and a student must force themselves into the program and into the mix of the lab in order to learn anything. I was "tested" by a HT veteran of 14 years who had to use Carson's textbook just to denote the color of collagen in a Trichrome stain. She then told my advisor that I passed the test and could even "pronounce all the big words without looking at the book!" Is this the REAL world of "old" Histology. Funny, I graduated with my first degree in the late '80's and I never met a researcher in Biological Sciences who required a textbook to test my knowledge. I have written to the Board of Registry and, of course, heard nothing. This is a sweeping and unjust move on a recent graduate and, even given the abilities, one may not be able to live, breathe and eat Histotechnology in order to meet a goal imposed at the eleventh hour. If anyone knows anything about this ruling and why these changes are being introduced so swiftly, please respond to KDrummey@msncom. If anyone can justify the ruling I would be most happy to hear from you and, if you are a Board Member, maybe you could reimburse my tuitions and expenses for the last two years I have spent proving that I remember what I learned 20 years ago and then justify the end of career that never got off the ground. Thank You...KDrummey@msn.com_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo.rogerson <@t> waht.swest.nhs.uk Mon Mar 20 04:30:35 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Mar 20 04:29:54 2006 Subject: [Histonet] Re: Recent changes in HT Certification Message-ID: I think what I find interesting is the idea that a HistoTech/ Biomedical scientist has to do a Practical. In the old days of 'Finals' that is exactly what you did; cut a section of breast on a desert island with an old tin trunk and razor blade and rum. But suddenly it changed and it all became theoretical. BSc(Hons) Biomedical Science or even MSc Biomedical Science has a practical element I concede but can we really say we passed a Practical Exam for something that is fundamentally a practical job? Gues it's Ok for Haematologists or Biochemists are they don't use their hands much, only for pushing buttons, but Microbiologists and Histologists still have to be 'practical', don't they? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Lee & Peggy Wenk [mailto:lpwenk@sbcglobal.net] Sent: Monday, March 20, 2006 10:04 AM To: 'Karen Drummey' Cc: 'Histonet' Subject: RE: [Histonet] Re: Recent changes in HT Certification Karen - First of all - congratulations on passing the written/computer portion of the exam. This is the hardest part for candidates to pass. Most people, if they pass the written, also pass the practical. So the hardest part is done for you. Now onto the practical part, which you needed to do anyway to become a certified histotech. It just looks like this will happen a little sooner than you had planned. I don't know if you saw my very long Histonet discussion on 03/14/2006, so I'm including it below, after my comments. Program Directors of NAACLS accredited HT/HTL programs were notified about 2 months ago via email, that the PRACTICAL portion of the HT and HTL exams was being dropped - that 2006 would be the last year it was required. In the future, just the written (computer) portion of the HT and HTL exam will be required to be a certified HT/HTL. Just like MT/MLT/CT only have to take their written portions to become certified - they don't have to take a practical exam. So the HT certification is NOT being discontinued. Just the practical requirement is being discontinued. The written/computer portion only will be given in the future. (Also, a little aside - as of January 2005, the window of taking and passing both parts (written and practical) of the HT/HTL exams was changed from 5 years to 3 years. Candidates who passed the written in 2005 (like yourself) now had 3 years in which to take and pass the practical exam. That should have been in the letter all 2005 candidates received, and program directors were notified of this change last year, also.) Please note - it was program directors of NAACLS accredited HT/HTL programs that were notified of the 3 year change and the practical change, NOT academic advisors at colleges. I don't know, maybe this is one and the same person for you. Maybe it's two different people. Concerning your statement that there is no established program in the US that culminates in the HT degree and the ASCP certification. That is correct. The ONLY way to become ASCP certified is to take and pass the ASCP Board of Registry (BOR) certification exam. It's the same way with nurses - they complete nursing school and then they take their Boards separately. There is no nursing school that automatically says "you are a Board certified nurse" immediately upon graduation. In order to take the HT/HTL exam, candidates must either: - complete a NAACLS accredited histotechnology program, OR - earn their college degree with the correct amount of biology and chemistry AND obtain 1 year full time histotechnology experience. What attending a NAACLS school does is allow someone to take the ASCP BOR certification exam IMMEDIATELY upon graduation from the NAACLS program, vs. having to earn an associate degree and then obtain 1 year full time histology experience (on-the-job experience (OJT)) before being allowed to take the HT exam (or having to earn a bachelor's degree and then obtain 1 year full time histology experience before being allowed to take the HTL exam). So attending a NAACLS accredited program shortens this time frame to take the certification exam by 1 year. Also, the majority of NAACLS graduates pass the HT/HTL exams, while the majority of OJT graduates do not pass. The passing score that you have earned on the written is NOT "obsolete one month after grading". That written/computer score is still good, but the practical portion still needs to be taken and passed before becoming a certified histotech. Passing only one portion is not enough to be considered "certified". Since the practical requirement is being dropped after 2006, all candidates who previously passed the written portion must now pass the practical by the end of the 2006, in order to be ASCP certified. Candidates have between now and the deadline for the November practical grading to cut and stain nine (9) slides. If candidates who previously passed the written don't take the practical in 2006, or if they fail the 2006 practical, they still can retake the written portion of the NEW HT exam in 2007 and pass that way. So there are still options out there for these candidates. Hopefully the lab where you are working has enough tissue variety for you to obtain the tissues for the practical portion. If you need any help obtaining some of the tissues that are required, let the Histonetters know. We've helped other people obtain the one or two "hard to get" tissues. As my comments from last week (see below) state, call Gerry Piscorski at ASCP BOR for more information about why the practical was dropped, or to talk about your concerns about your status. She is definitely willing to talk with people. This is definitely a big change for the histotech community. As for your concerns about the quality of your training, please call NAACLS (773-714-8880). They are the agency that accredits HT/HTL programs (as well as MT/MLT/etc.). They need to hear your concerns and problems with the rotation part of your program. Comments from last week below. Peggy A. Wenk, HTL(ASCP)SLS Program Director, HT and HTL Programs William Beaumont Hospital Royal Oak, MI 48073 - - - COMMENTS FROM LAST WEEK: Yes, the HT and HTL practical exams are being discontinued starting in 2007. In other words, 2006 is the last year it will be required. (If you're not interested, please hit "delete" at this point, as I'm going to get wordy.) There was information on the ASCP Board of Registry (BOR) web page. However, ASCP recently redid their entire web page, and this information is in queue to be put back up on the new pages. (In other words, they didn't drop it, it just hasn't been put back up.) I'll fill you in on what I know, learned by reading an email sent to all HT/HTL program directors about 2 months ago, and what was talked about at educator's forum at the 2005 NSH meeting in Florida. Also, I talked with Dr. Blair Holladay (head of ASCP BOR) when the email first came out, and with Gerri Piscorski (exam manager) (312-541-4887), after that, to get more information. Histotechs were the only category still required to do a practical. Med techs don't have to prove they can make a blood smear or can do a Gram stain or can streak an agar plate. Cytotechs don't have to prove they can make a FNA smear or do a Pap stain. Phlebotomists don't have to prove they can get blood from someone with collapsed veins, or do a heel stick on a newborn. All other categories are tested by just a written exam. The majority of histotechs pass the practical portion. Of those that don't pass the practical, the majority of these also did not pass the written portion. In other words, very few people pass the written but fail the practical. Most either pass the practical/fail the written, or they fail both parts, or they pass both parts. So the written portion of the HT/HTL exam was a better indicator of who would pass/fail both parts, than the practical exam. The cost of the grading of the practical exams was very high - flying histotechs and pathologists into Chicago from all over the country, putting them up in hotels for the weekend, feeding them. The graders were not being extravagent - pizza for lunch, sharing cabs to the grading center, etc. The number of slides was reduced from 15 to 9, in part to reduce the number of graders needed, thus reducing the cost. (By statistically picking the right combination of tissues and stains, 9 slides were giving the same pass/fail rate as the previous 15 slides.) The fee charged to the HT/HTL candidates did not cover the cost of grading the practical exam. Not even when an additional $75 fee was added. So ASCP BOR was losing money with each practical exam. Due to concerns about HIPAA, confidentiality, shipping, etc., many candidates were having difficult times obtaining tissue (especially students in college based histotech programs, that were doing rotations in hospital labs for, say, 2 months). This idea of dropping the practical exam has been around for quite a few years. Reducing the number of slides on the practical from 15 to 9 was a compromise, a stepping stone if you would. The people on the ASCP BOR Histotechnology Exam Committee have been taking pictures of poor staining and sectioning artifacts. These will be incorporated into future written exams as troubleshooting and problem-solving questions. What are the causes and how to correct thick/thin sections, wrinkles, folds, splits, microchatter, etc. So the exam is being re-written to incorporate more staining and sectioning problems. Remember, the ASCP BOR Histology Exam Committee with histotechs very involved with NSH, were involved in this discussion/decision. NSH Board has a representative on the Histology Exam Committee/ASCP Board of Governors, who was involved with this topic. ASCP BOR also talked with program directors of HT/HTL schools about this at the NSH convention, asking their opinion. And this topic has been discussed at various committees that I've been on, for the past several years. So it's been coming, slowly, for somewhere over the last 6 years, maybe closer to 10 years, that I'm aware of. Now, some questions you may be having - What is going to happen to the people who didn't pass the practical last year or the year before (2005, 2004)? Each one has been sent a letter, informing them at they must take and pass the practical in 2006 (this year). If they don't pass the practical this year, then they will not have passed their HT or HTL exam. - What about the people taking the HT/HTL exam for the first time this year (2006), who don't pass? They will be given one more year (2007) to take the "make up" practical, in order to pass their HT/HTL exam. So, for just these few people, they will be allowed to take the practical in 2007. But no one else. - How will I know if someone I'm thinking about hiring can really cut or stain, if they don't have to do a practical exam for ASCP? My suggestion - having them do a "practical exam" as part of their interview with you. Hand them 6 blocks, put them in front of a microtome, and give them 20 minutes to section. Then interview them more (to use up time during the drying), and have them load the H&E stainer. While the slides are staining, have a folder of slides for them to look at - some H&E, some special stains. Ask them to identify the stains or the tissue or what's wrong with a poorly stained slide. As long as you use the same type of tissues in the blocks, and the same stained slides, and have EVERY candidate do the test - this is perfectly acceptable way to assess someone you are interviewing for a position. By this time, the H&Es are done. Have them coverslip them. If you still have questions, PLEASE contact Gerry (see direct phone number above). She is very willing to talk with people about this (or any other question you have about the HT/HTL exams. ASCP is THE best place to go to for questions about ASCP. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Karen Drummey Sent: Sunday, March 19, 2006 7:20 PM To: histonet Subject: [Histonet] Re: Recent changes in HT Certification Evening: I am curious if anyone out there is aware of the reasoning behind the discontinuation of the ASCP certification for HT's as of December 2006? I was told, upon graduation, that there was a five year window within which to become registered. I graduated many years ago with a B.A., M.A.(eq) but returned to study for the AAS in Applied Lab Science and graduated with a 4.0 in Histotechnology in May 2005. I took the computerized test immediately and passed with a very high score. So many histotechs are needed; however, most of the hiring managers (many of whom I hope read this E-mail) base everyone's skill level on their current education ONLY and the lack of an ASCP certification. There is no established program in the Continental United States that culminates in the acquisition of both the HT (or HTL) degree and the ASCP certification. I welcome being corrected if someone knows of such a program. It took me several months to gain employment and I am limited in time and acquisition of tissue at this point. I was not too concerned because I believed that I had ample time to become registered. I received a letter last week from the Board of Registry directing me to sign up immediately for the November practical because the ASCP would no longer be available to HT graduates after December 2006. My main question is ....What are the implications of this move? What good is an ASCP certification if it is obsolete one month after grading? My academic advisor was not made aware of this move until I wrote and called. The program coordinators are not being informed by the Board of Registry and it is simply not just for people to be sucked into a program, allowed to pay for an education only to graduate without the ability to get the certification they will be asked for just to be considered for a hospital or lab based position. I have found the whole experience frustrating and in a constant state of flux. The HT's and HTL's with years of experience believe that all newcomers are academicians who cannot perform the tasks of the lab. The problem that I have seen is that there are no real "teachers" or instructors left in the world of Histotechnology. Everyone is an administrator and a student must force themselves into the program and into the mix of the lab in order to learn anything. I was "tested" by a HT veteran of 14 years who had to use Carson's textbook just to denote the color of collagen in a Trichrome stain. She then told my advisor that I passed the test and could even "pronounce all the big words without looking at the book!" Is this the REAL world of "old" Histology. Funny, I graduated with my first degree in the late '80's and I never met a researcher in Biological Sciences who required a textbook to test my knowledge. I have written to the Board of Registry and, of course, heard nothing. This is a sweeping and unjust move on a recent graduate and, even given the abilities, one may not be able to live, breathe and eat Histotechnology in order to meet a goal imposed at the eleventh hour. If anyone knows anything about this ruling and why these changes are being introduced so swiftly, please respond to KDrummey@msncom. If anyone can justify the ruling I would be most happy to hear from you and, if you are a Board Member, maybe you could reimburse my tuitions and expenses for the last two years I have spent proving that I remember what I learned 20 years ago and then justify the end of career that never got off the ground. Thank You...KDrummey@msn.com_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Mon Mar 20 09:28:54 2006 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Mon Mar 20 09:29:02 2006 Subject: [Histonet] mucicarmine Message-ID: <0E394B648E5284478A6CCB78E5AFDA270171FCE1@hpes1.HealthPartners.int> We have been doing our mucicarmine on the Nexus special stainer and due to the fact that we do not do a lot of these, I decided to bring it back to handstaining. I am using the Southgate's modification of Mayer's Technique and having a very hard time getting the stain to look nice and sharp! Does anyone have any procedures they use or suggestions as to what could be our problem? Our muci is not staining the bright red and even the nuclei are not stained very dark. We have tried various times and strengths of the mucicarmine and even from two different companies! Help and thanks for any and all suggestions! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From anne-sophie.martinez <@t> unicaen.fr Mon Mar 20 09:55:25 2006 From: anne-sophie.martinez <@t> unicaen.fr (Anne-Sophie MARTINEZ) Date: Mon Mar 20 09:52:41 2006 Subject: [Histonet] TEM and oysters Message-ID: <441ED06D.1020404@unicaen.fr> Hi everybody. I have 2 questions concerning TEM. (1) I have fixed gonads of adult oysters for TEM with glutaraldehyde, post-fixed with osmium and done the embedding in Epon. Is there any way I can you do immunocytochemistry with antibodies on these samples? (2) How can I decalcify the shelves of larvae and juveniles oysters for TEM without damaging the tissue? Could anyone help me please? Thanks for your suggestions. Anne-Sophie From ROrr <@t> enh.org Mon Mar 20 10:11:28 2006 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Mon Mar 20 10:11:33 2006 Subject: [Histonet] MT's and HT's Message-ID: Thank you to each and everyone who replied to me privately on the Issue of HT's and MT's as managers. It turned from a warm topic to a very HOT topic. Someone asked for some feedback, because a lot of the respondents did not want to publicize their opinions. I'll be very general here. Most of the opinion is that all the MT's that are managers were trained and had a propensity for Histology work. Most took pay cuts to work as histo techs. Most commented that the better salaries arrived with management positions. I have received comments from many saying MT's cannot perform with the same skills as an HT. I also received the exact opposite, that they are MORE capable! There is varying opinion about the COMPLEXITY of testing in the histology lab. There is also varying opinion about critical thinking skills that are learned in a formal setting(university) and those same skills developed specifically on the job (HT with 10 years experience.) The pay in the histo lab seems to be lower because the complexity of testing is presumed to be less. That's the feedback, not necessarily my opinion. Thanks again to everyone. I've gotten the feedback I needed. Becky Becky Orr CLA,HT(ASCP) IHC Lead Evanston Northwestern Healthcare 847-570-2771 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Sunday, March 19, 2006 11:25 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 28, Issue 31 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. CD4 & CD8 by Immunohistochemistry (Marilyn Johnson) 2. lectin stains and antigen retrival (Franz-Josef M?ller) 3. Re: lectin stains and antigen retrival (Rene J Buesa) 4. Florida Society For Histotechnology Annual Meeting (Santiago, Jerry) 5. cd8 on rats (pruegg@ihctech.net) ---------------------------------------------------------------------- Message: 1 Date: Sat, 18 Mar 2006 17:17:58 -0700 From: "Marilyn Johnson" Subject: [Histonet] CD4 & CD8 by Immunohistochemistry To: Message-ID: <004401c64aea$93c4d1b0$6501a8c0@VALUED20606295> Content-Type: text/plain; charset="iso-8859-1" Hi Histonetters, In approximately two months, our Lab. will be initiating a new project using skin sections of mice. We will be using IHC to demonstrate infiltrates at vaccine sites including CD4, CD8, B-cells, monocytes, macrophages, etc. Any assistance would be greatly appreciated. Thank you in advance. Marilyn Johnson Alberta Agriculture Edmonton, Alberta, Canada ------------------------------ Message: 2 Date: Sun, 19 Mar 2006 09:18:20 +0100 From: Franz-Josef M?ller Subject: [Histonet] lectin stains and antigen retrival To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1; delsp=yes; format=flowed Dear Histonetters, does anybody know if lectin stains are stable after the tissue section (20u, paraffin embedded) was subjected to heat antigen retrieval in citric acid pH 6.0 ? Thanks for your help in advance! Cheers franzef Dr. med. Franz-Josef M?ller Zentrum f?r Integrative Psychiatrie, Kiel Zellbiologisches Labor Niemannsweg 147 24105 Kiel GERMANY Tel.: +49 431 597 4169 ------------------------------ Message: 3 Date: Sun, 19 Mar 2006 07:35:56 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] lectin stains and antigen retrival To: Franz-Josef "M?ller" , histonet@lists.utsouthwestern.edu Message-ID: <20060319153557.93612.qmail@web61214.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Franz-Josef: Lectins, as you know, will react with carbohydrates in the tissue. The lectin will attach to the carbohydrates, and the anti-lectin-antibody will bind to the lectin identifying the carbohydrates sites in a 2 steps (indirect) reaction. Since carhohydrates are not affected with formalin fixation as proteins do, it is not necessary to subject the sections to antigen retrieval (HIER) this being a step you can avoid if just trying to use the lectins. They should be stable after HIER since some carbohydrate detecting techniques (like PAS) are done in acid pH solutions. Hope this will help! Ren? J. Franz-Josef M?ller wrote: Dear Histonetters, does anybody know if lectin stains are stable after the tissue section (20u, paraffin embedded) was subjected to heat antigen retrieval in citric acid pH 6.0 ? Thanks for your help in advance! Cheers franzef Dr. med. Franz-Josef M?ller Zentrum f?r Integrative Psychiatrie, Kiel Zellbiologisches Labor Niemannsweg 147 24105 Kiel GERMANY Tel.: +49 431 597 4169 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Mail Bring photos to life! New PhotoMail makes sharing a breeze. ------------------------------ Message: 4 Date: Sun, 19 Mar 2006 11:23:43 -0500 From: "Santiago, Jerry" Subject: [Histonet] Florida Society For Histotechnology Annual Meeting To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" The Florida Society for Histotechnology is preparing for their Annual Symposium May 5 - 7, 2006 Embassy Suites Deerfield Beach Florida. All Information regarding registration, hotel and exhibiton can be accessed through our website at www.fshgroup.org. Online registration is now available.We will be offering 16 workshops and the exhibit will consist of approximately 40 exhibitors. Exhibitors can download an exhibiton form from the website and mail to the address printed. Remember this is a licensure renewal year and all your mandatory's will be offered during this symposium. Any questions please feel free to contact Susan Clark at 954-987-2020 X 5371 ------------------------------ Message: 5 Date: Sun, 19 Mar 2006 10:33:42 -0700 From: pruegg@ihctech.net Subject: [Histonet] cd8 on rats To: Message-ID: <200603191733.k2JHXh46094702@pro12.abac.com> Content-Type: text/plain; charset="us-ascii" OK, I think I have some good advise on doing CD8 on frozen rat tissue, now has anyone gotten CD8 to work of ffpe rat tissue yet. I know, I know, this question comes up all the time for mice and I have been given procedures that I struggled over with no success, but I was hoping it might be more promising for rats. Patsy BTW Those signed up to the IHCRG list serve. We are switching host servers so be patient while we get back up to speed. Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net website www.ihctech.net ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 28, Issue 31 **************************************** From rjbuesa <@t> yahoo.com Mon Mar 20 10:19:02 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 20 10:19:06 2006 Subject: [Histonet] mucicarmine In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA270171FCE1@hpes1.HealthPartners.int> Message-ID: <20060320161902.88485.qmail@web61216.mail.yahoo.com> Are you using the left-over of the not so frequently used staining kit? How old are the solutions? I always stained nuclei first, followed by the mucicarmin sol. (35 secs. boost in the MW oven) followed by 1 hour at 60?C in the water bath. From that moment on everything should be done as quickly as possible: rinse in water, quick rinse in 95% ethanol; quickly (only a few seconds) in a strong (2% alc.) fast green solution; followed by a very fast dehydration/clearing (only 1-2 dips in the alcohols). Hope this will help you. Ren? J. "Webb, Dorothy L" wrote: We have been doing our mucicarmine on the Nexus special stainer and due to the fact that we do not do a lot of these, I decided to bring it back to handstaining. I am using the Southgate's modification of Mayer's Technique and having a very hard time getting the stain to look nice and sharp! Does anyone have any procedures they use or suggestions as to what could be our problem? Our muci is not staining the bright red and even the nuclei are not stained very dark. We have tried various times and strengths of the mucicarmine and even from two different companies! Help and thanks for any and all suggestions! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Mail Use Photomail to share photos without annoying attachments. From NMargaryan <@t> childrensmemorial.org Mon Mar 20 10:36:41 2006 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Mon Mar 20 10:35:36 2006 Subject: [Histonet] (no subject) Message-ID: <63B8B599DE283148B92E83C78B32C15D01C76AE2@cmhexbe2.childrensmemorial.org> Hi Histonetters, I will be using IHC to demonstrate CD34 and PSMA expression on human prostate sections. Any assistance with best Abs and protocols would be greatly appreciated. Thank you in advance, Naira Naira V. Margaryan, D.V.M., Ph.D. Research Associate III Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3394 Tel: 773-755-6570/ext-8 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 From pruegg <@t> ihctech.net Mon Mar 20 10:58:17 2006 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Mon Mar 20 10:58:30 2006 Subject: [Histonet] FW: csh meeting April 28-29,2006 Message-ID: <200603201658.k2KGwFWg069286@pro12.abac.com> JOIN THE COLORADO SOCIETY FOR HISTOTECHNOLOGY AT OUR STATE MEETING BEING HELD APRIL 28 AND 29, 2006 IN BEAUTIFUL COLORADO SPRINGS, CO. Cheyenne Mountain Resort 3225 Broadmoor Valley Road Colorado Springs, CO 80906 Ph: (800) 428-8886 www.cheyennemountain.com ATTENDEES AND VENDORS CAN REGISTER ONLINE AT: WWW.COLORADOHISTO.ORG PROGRAM SESSION 1: FRIDAY AFTERNOON, APRIL 28, 2006 1:00 PM - 2:30 PM, Presentations 1-A. What is radio frequency energy? How its use during surgery can cause staining artifacts. Ms. Janet Maass, HT, HTL - Valley Lab, Boulder, CO This workshop is a brief explanation of what RF energy is and how its use in surgery affects histological stains. CEU credit hours: 1.5 1-B. Unsolvable Mysteries I Ms. Deb Flynn, HT, QIHC - Technical Marketing Rep.,- Ventana Medical Systems, Tucson, AZ. Mr. Mike Reichenbach, HT, QIHC - Senior Technical Marketing Rep. - Ventana Medical Systems, Tucson, AZ. This workshop will give attendees a better understanding of how complex or "unsolvable" cases can be diagnosed, leading to a better course of treatment for patients. CEU credit hours: 1.5 _____ 2:30 PM - 3:00 PM, Break _____ 3:00 PM - 4:30 PM, Presentations 2-A. A Family Affair - The Formation & Development of Team Histology Mr. H. Skip Brown, B.A., HT (ASCP), Applications Manager - McCormick Scientific, St. Louis,MO. This is a workshop on learning how to work in a team dynamic in Histology. CEU credit hours: 1.5 2-B. Unsolvable Mysteries II Ms. Deb Flynn, HT, QIHC - Technical Marketing Rep.,- Ventana Medical Systems, Tucson, AZ. Mr. Mike Reichenbach, HT, QIHC - Senior Technical Marketing Rep. - Ventana Medical Systems, Tucson, AZ. This workshop will give attendees a better understanding of how complex or "unsolvable" cases can be diagnosed, leading to a better course of treatment for patients. CEU credit hours: 1.5 4:30 PM - 7:00 PM, Social SESSION 2: SATURDAY MORNING, APRIL 29, 2006 8:30 AM - 10:00 AM, Presentations 3-A. Basic Dynamics of Fixation & Processing Mr. H. Skip Brown, B.A., HT (ASCP), Applications Manager - McCormick Scientific, St. Louis,MO. This workshop will cover the theory of fixation/processing, dynamics of fluid transfer in tissue, optimal or end point and microwave fixation &processing. Different instruments and cassettes that enhance fluid transfer will be introduced. CEU credit hours: 1.5 3-B. Variant Creutzfeldt Jakob Disease: Not your classic CJD Ms. Konnie Zeitner, HT, HTL, SLS - Anatomic Pathology Manager Nebraska Med. Ctr., Omaha, NE This workshop is a discussion on a variant CJD and how it differs from classic CJD in presentation and length of time from onset to death. CEU credit hours: 1.5 _____ 10:00 AM - 10:30 AM, Break SESSION 2: SATURDAY MORNING, APRIL 29, 2006 10:30 AM - 12:00 PM, Presentations 4-A. Determining the Post Mortem Interval (PMI) Dr. Stephen Cina, M.D., Weld County Coroner, Pathologist, Mckee Med. Ctr., Loveland, CO This workshop will delve into what the PMI is and how it is determined off of a "best estimate" rather than a finite determination. (Graphic material to be presented) CEU credit hours: 1.5 4-B. Am I Really Ready for this CAP Inspection Ms. Christine "Charlie" Dorner, HT, QIHC - Immuno Manager - Vision Biosytems, Norwell, MA. Ms. Leslie Kerr, Field Support Specialist - Vision Biosytems, Norwell, MA. This workshop will cover preparation for the first or repeat CAP inspection. The topics include: preparation, new questions, and overall preparation for the big inspection. It will be open format for participants to discuss past or present concerns. CEU credit hours: 1.5 _____ 12:00 PM - 1:30 PM Lunch & CSH Business Meeting _____ SESSION 3: SATURDAY AFTERNOON, APRIL 29, 2006 1:30 PM - 3:00 PM, Presentations 5-A. Basic Tissue Gross Dissection and Description I Mr. Lance Erickson, HTL- Anatomic Path Supervisor Primary Children's Med. Ctr., Salt Lake, UT This workshop is a basic overview of the requirements, techniques, and specimen standards of gross dissection and description. CEU credit hours: 1.5 5-B. From Bench Tech to Manager: Be Successful by Valuing People I Ms. Konnie Zeitner, HT, HTL, SLS - Anatomic Pathology Manager Nebraska Med. Ctr., Omaha, NE This workshop will present ideas on focusing on people and principles, developing communication skills and believing your own personal potential in making the move from Tech to supervisor and or manager. CEU credit hours: 1.5 _____ _____ 3:00 PM - 4:30 PM, Presentations 6-A. Basic Tissue Gross Dissection and Description II Mr. Lance Erickson, HTL- Anatomic Path Supervisor Primary Children's Med. Ctr., Salt Lake, UT This workshop is a basic overview of the requirements, techniques, and specimen standards of gross dissection and description. CEU credit hours: 1.5 6-B. From Bench Tech to Manager: Be Successful by Valuing People II Ms. Konnie Zeitner, HT, HTL, SLS - Anatomic Pathology Manager Nebraska Med. Ctr., Omaha, NE This workshop will present ideas on focusing on people and principles, developing communication skills and believing your own personal potential in making the move from Tech to supervisor and or manager. CEU credit hours: 1.5 _____ Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net website www.ihctech.net From gcallis <@t> montana.edu Mon Mar 20 11:36:44 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Mar 20 11:36:48 2006 Subject: [Histonet] CD 31 sheep In-Reply-To: References: Message-ID: <6.0.0.22.1.20060320103451.01b65510@gemini.msu.montana.edu> There is a clone from SEROTEC, according to their veterinary antibody chart that is supposed to work - CO.3E1D4, if this is what you are looking for. No other information, unless you contact SEROTEC technical services for particulars. At 03:55 PM 3/17/2006, you wrote: >Does anyone have a working Antibody and protocol for CD 31 in Sheep? > >-Tom Wood >Diagnostic Center for Population and Animal Health >Michigan State University >Wood@dcpah.msu.edu Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From wood <@t> dcpah.msu.edu Mon Mar 20 11:53:21 2006 From: wood <@t> dcpah.msu.edu (Thomas Wood) Date: Mon Mar 20 11:56:21 2006 Subject: Fwd: [Histonet] CD4 & CD8 by Immunohistochemistry Message-ID: Invitrogen (formerly Caltag) has a B-cell marker that works great on mouse tissue it is CD45r (B220) both purified and biotin labelled. I used it on our new Bond Max autostainer from Vision Bio Sytems and had excellant results with FFPE tissue. -Tom Wood Michigan State University Diagnostic Center for Population and Animal Health >>> "Marilyn Johnson" 03/18/06 07:17PM >>> Hi Histonetters, In approximately two months, our Lab. will be initiating a new project using skin sections of mice. We will be using IHC to demonstrate infiltrates at vaccine sites including CD4, CD8, B-cells, monocytes, macrophages, etc. Any assistance would be greatly appreciated. Thank you in advance. Marilyn Johnson Alberta Agriculture Edmonton, Alberta, Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Mar 20 11:56:13 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Mar 20 11:56:24 2006 Subject: [Histonet] (no subject) In-Reply-To: <63B8B599DE283148B92E83C78B32C15D01C76AE2@cmhexbe2.childrensmemorial.org> Message-ID: <20060320175613.75065.qmail@web61217.mail.yahoo.com> Naira: I used CD34 from Becton Dickinson, HIER citrate buffer at pH6 ; 1:100 dilution, placenta as control with Dako autostainer and EnVision+(detection)System. Never used PSMA Hope this will help you. Ren? J. "Margaryan, Naira" wrote: Hi Histonetters, I will be using IHC to demonstrate CD34 and PSMA expression on human prostate sections. Any assistance with best Abs and protocols would be greatly appreciated. Thank you in advance, Naira Naira V. Margaryan, D.V.M., Ph.D. Research Associate III Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3394 Tel: 773-755-6570/ext-8 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Mail Bring photos to life! New PhotoMail makes sharing a breeze. From gcallis <@t> montana.edu Mon Mar 20 12:11:18 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Mar 20 12:11:22 2006 Subject: [Histonet] lectin stains and antigen retrival In-Reply-To: References: Message-ID: <6.0.0.22.1.20060320105732.01b79d30@gemini.msu.montana.edu> Yes, lectins are stable although trypsin enzyme digestion at 37C may be better than citrate buffer retrieval. Also, there are buffer considerations for staining, i.e. TRIS Lectin buffer with Mg and Ca added. The book gives the recipe for this and with some lectins PBS should be avoided and also normal serums. AND there is an excellent book on Lectin Histochemistry , a concise practical handbook SA Brooks et al, ISBN#1859961002 that gives IHC staining methods, etc. It is not expensive and Springer Verlag is the publisher source. 01:18 AM 3/19/2006, you wrote: >Dear Histonetters, >does anybody know if lectin stains are stable after the tissue >section (20u, paraffin embedded) was subjected to heat antigen >retrieval in citric acid pH 6.0 ? >Thanks for your help in advance! >Cheers >franzef > >Dr. med. Franz-Josef M?ller >Zentrum f?r Integrative Psychiatrie, Kiel >Zellbiologisches Labor >Niemannsweg 147 >24105 Kiel >GERMANY > >Tel.: +49 431 597 4169 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From PMonfils <@t> Lifespan.org Mon Mar 20 12:24:09 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Mon Mar 20 12:24:22 2006 Subject: [Histonet] TEM and oysters Message-ID: <09C945920A6B654199F7A58A1D7D1FDE017176A2@lsexch.lsmaster.lifespan.org> (1) For most types of antigens, not likely. Glutaraldehyde crosslinks proteins so aggressively that most antigenicity is greatly reduced or completely destroyed. If immunolabeling is the objective before the fact, the tissue should be fixed very conservatively, for example 0.25% glutaraldehyde for a half hour to one hour. Following normal glutaraldehyde fixation with osmium introduces additional problems, as osmium greatly denatures proteins, even those crosslinked by glutaraldehyde, and actually dissolves some pre-fixed proteins out of the tissue by breaking the glutaraldehyde bonds. The only successful attempt at immunolabeling glut/osmium fixed tissue that I can recall was a paper written by David Knibbs around 1992-1994 in which he described a method for immunolabeling (using colloidal gold-labeled antibodies) various kinds of intermediate filaments. I believe the abstract of that paper would be in the proceedings of the American Society of Cell Biology. (2) You cannot "decalcify" mollusc shells in the same sense that you can decalify bone because bone consists of a solid tissue matrix infused with calcium salts, while a molluscan shell is composed almost completely of calcium salts deposited by the animal's mantle, with only intermittent traces of protein. So, if you expose the shell to decalcifying agents, the shell will dissolve completely. If the loss of the shell is not a problem, then it can be dissolved away much more rapidly than a bone of equivalent size can be decalcified, since the bone matrix presents a barrier to the penetration of the decalcifying solution. When I taught chemistry years ago, I did a demo by filling a 1000 ml glass cylinder with 10% hydrochloric acid and dropping in a clam shell. A dense cloud of bubbles would immediately erupt, causing the liquid to "boil" as the calcium carbonate of the shell was transformed into carbon dioxide, and the shell would be completely gone in about two minutes. So, theoretically any decalcifying method could be used to remove the shell from a molluscan specimen. If you consider acid methods too harsh, I would recommend a chelation method such as disodium EDTA. I never tried this on a mollusc shell, but I see no reason why it wouldn't work. You'll have to experiment to find an optimum exposure time, but again it should be much shorter than the time required for decalcifying bone. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > Anne-Sophie MARTINEZ > Sent: Monday, March 20, 2006 7:55 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] TEM and oysters > > Hi everybody. I have 2 questions concerning TEM. > (1) I have fixed gonads of adult oysters for TEM with glutaraldehyde, > post-fixed with osmium and done the embedding in Epon. Is there any way > I can you do immunocytochemistry with antibodies on these samples? > (2) How can I decalcify the shelves of larvae and juveniles oysters for > TEM without damaging the tissue? > Could anyone help me please? Thanks for your suggestions. > Anne-Sophie > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From TJJ <@t> Stowers-Institute.org Mon Mar 20 12:47:51 2006 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Mon Mar 20 12:48:10 2006 Subject: [Histonet] Re: mucicarmine Message-ID: Dorothy, It's been a while since I've done one, but I remember we got our best results using Weigert's Iron Hematoxylin staining first, and then using Sigma's mucicarmine stain. I don't quite remember if we used it undiluted, but I think we did use it more concentrated than their protocol called for. Where I trained, we made up the mucicarmine stain and used undiluted stock to get brighter pink/rose staining results. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64133 From TJJ <@t> Stowers-Institute.org Mon Mar 20 12:58:05 2006 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Mon Mar 20 12:58:31 2006 Subject: [Histonet] 2006 Missouri Symposium - June 2-3 St. Louis, MO Message-ID: If anyone is interested in coming to our fantastic meeting and you need the registration forms and other information, please contact: Sharon Walsh (MO Society President): userwalsh@aol.com Teri Johnson (Exhibit coordinator): tjj@stowers-institute.org Rosetta Barkley (Registrar): rbarkley2@kumc.edu Our deepest gratitude is extended to all the exhibitors, sponsors and speakers that make the MSH meeting possible. MISSOURI SOCIETY FOR HISTOTECHNOLOGY Presents Meet me in St. Louis The 29th Annual Spring Symposium For Continuing Education and Histopathologic Technique June 2-3, 2006 Embassy Suites Hotel-St. Louis Downtown GENERAL INFORMATION Date: June 2-3, 2006 Meeting Location: Embassy Suites Hotel-St. Louis Downtown 901 North 1st Street St. Louis, Missouri 63102 314-241-4200 www.stlouisdowntown.embassysuites.com Room Reservations should be made directly with the Embassy Suites, St. Louis, MO. Hotel reservations deadline is May 2, 2006. To secure the symposium rates, make your reservations early. The Chicago Cubs and the Cardinal's are playing this weekend in St. Louis, no rooms will be held after May 2nd, and it will be almost impossible to get a room in St. Louis. Make your reservations early. Room Rates: $119.00 Thursday (6/1) and Friday (6/2) Each suite is beautifully decorated with a private bedroom and spacious living room. All of our suites are fully equipped with two televisions, a refrigerator, microwave oven, coffee maker, two telephones with data ports and a well lit dining/work table. The lobby atrium provides guests a complimentary full cooked-to-order breakfast and an Evening Managers Reception including your favorite beverages every evening. (Free food and Drink) Attractions include the President Casino and the St.Louis Arch. SCHEDULE Friday, June 2, 2006 7:30 - 8:00 REGISTRATION 8:15 - 8:30 President Message- Sharon Ann Walsh 8:30 - Noon SCIENTIFIC SESSIONS 8:30 - 9:15 Reagent Alcohol, What's it all about? Pamela Marcum, University of PA, School of Veterinary Medicine, Kennett Square, PA (Sponsored by Poly Scientific) 9:15 - 10:00 Importance of Special Stains in Renal Pathology Cherese Cortese, M.D., Associate Professor, Department of Pathology St. Louis University, St. Louis, Missouri 10:00 - 11:00 COFFEE BREAK & EXHIBITS 11:00 - 11:45 EM for the Histotechnologist Randy D. Tindall, EM Specialist, Electron Microscopy Core Facility, University of Missouri, Columbia 11:45 - 1:00 AWARDS LUNCHEON: Region V Update, Konnie Zeitner WORKSHOPS 1:30 - 4:30 3:00 - 3:30 COFFEE BREAK & EXHIBITS Workshop #1 Double Immuno Staining Wet Workshop, Tara Kennedy, Applications Specialist, Biocare Medical Workshop #2 Histology Nuts and Bolts. Back to the Basics, Mari Ann Mailhiot BA HT(ASCP), Specimen Applications Specialist, Leica Microsystems Workshop #3 Stars of the Silver Screen, Trouble shooting Silver Stains, Joan M. Vesey HT(ASCP), Richard-Allan Scientific 5:30-7:30 P.M. Upper Garden Atrium, Embassy Suites Pizza Party Saturday, June 3, 2006 7:30 - 8:00 REGISTRATION 8:00 - 8:15 Saturday Welcome- Sharon Ann Walsh, President MSH 8:30 - 11:30 SCIENTIFIC SESSIONS 8:30 - 9:30 Basics for using Pro-EX-C in the Detection of High Grade SC from Atypical Squamous Metaplasia Lourdes Ylagen, Department of Pathology and Immunology, Division of Surgical Pathology, Washington University School of Medicine, St. Louis, Missouri 9:30 - 10:30 Safety in the Laboratory, What we take for granted. Sylvia Casey 10:30-11:00 MSH Business Meeting (The meeting will be held in the general session room immediately following the scientific session. All are welcome to attend). 11:00 - 12:30 Exhibit Hall Open. (Ballpark Break) WORKSHOPS 1:00 - 4:00 Workshop #4 Preparing for a CAP Inspection, Charlie Dorner, Vision Biosystems Workshop #5 Histologic Techniques in Hematopathology, Clinical Applications and Troubleshooting, Sharad Mathur, M.D., VAMC, Kansas City, Stacey Merica, HT(ASCP), VAMC, Kansas City Workshop #6 Is your Processor Fighting You, Fight Back! Pamela Marcum, University of PA, School of Veterinary Medicine, Kennett Square, PA (Sponsored by Poly Scientific) All workshops are CEU approved. Advance Registration must be postmarked by May 27th, 2006 (no refunds after this date). From DWilliams <@t> ciit.org Mon Mar 20 14:08:30 2006 From: DWilliams <@t> ciit.org (Delorise Williams) Date: Mon Mar 20 14:08:38 2006 Subject: [Histonet] North Carolina Spring Mtg. Message-ID: <12816CB59E68184F9F5F28063E68B04702A60D85@xsrvr.ciit.org> The North Carolina Society of Histopathology Technologists will be hosting their annual spring meeting at the Greensboro/ Highpoint Airport Marriott in Greensboro, NC. on April 21-22, 2006. We welcome you to come and experience North Carolina's beautiful springtime scenery. We have a very interesting program planned with such presenters as Peggy Wenk, Robert Lott, Lamar & Wanda Jones, Connie Wavrin and John Wilson from Ventana. For more info, contact me at dwilliams@ciit.org Delorise Williams CIIT Centers for Health Research PO Box 12137 Research Triangle Park, NC 27709 (919) 558-1200 Voice Mail-(919) 558-1252 Fax-(919) 558-1300 From Kathy.Johnston <@t> CLS.ab.ca Mon Mar 20 15:25:30 2006 From: Kathy.Johnston <@t> CLS.ab.ca (Kathy.Johnston@CLS.ab.ca) Date: Mon Mar 20 15:25:36 2006 Subject: [Histonet] Re: mucicarmine Message-ID: <4BC300747AF87A48BCDF8E48BC2885CE013652F2@mail1.calgary.com> We use the same stock here (Sigma) and dilute it 1:3 with dH2O. We do use Harris hematoxylin instead though. I have heard that you can use a tartrazine counterstain to emphasize the mucin staining if that is something your pathologists prefer. Kathy Johnston Tech II Special Stains Anatomic Pathology- DSC Calgary Laboratory Services 770-3572 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Johnson, Teri Sent: March 20, 2006 11:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: mucicarmine Dorothy, It's been a while since I've done one, but I remember we got our best results using Weigert's Iron Hematoxylin staining first, and then using Sigma's mucicarmine stain. I don't quite remember if we used it undiluted, but I think we did use it more concentrated than their protocol called for. Where I trained, we made up the mucicarmine stain and used undiluted stock to get brighter pink/rose staining results. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64133 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify the sender immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. From gcallis <@t> montana.edu Mon Mar 20 15:28:46 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Mar 20 15:28:50 2006 Subject: [Histonet] Human CD31, CD34, von Willebrands etc publication Message-ID: <6.0.0.22.1.20060320140932.01b0a7a8@gemini.msu.montana.edu> There has been a great deal of discussion about some of these markers and this publication in J Histochemistry Cytochemistry just came out. If you need a copy, I will happy to forward a pdf of the article privately. Immunohistochemical Expression of Endothelial Markers CD31, CD34, von Willebrand Factor, and Fli-1 in Normal Human Tissues Marc P. Pusztaszeri, Walter Seelentag and Fred T. Bosman Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From gcallis <@t> montana.edu Mon Mar 20 16:19:22 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Mar 20 16:19:35 2006 Subject: [Histonet] Rat CD8 marker attn: Patsy Ruegg Message-ID: <6.0.0.22.1.20060320151602.01b0b040@gemini.msu.montana.edu> Patsy, There is an excellent article (even though from 1995) on Rat CD8 (OX 8) along with other rat CD markers and murine CD markers. The authors used PLP as the fixative for paraffin embedded tissue. Immunohistochemical detection of T cell subsets and other leukocytes in paraffin embedded rat and mouse tissues with monoclonal antibodies. J Histochem Cytochem 45(3):313-320,1995 Whiteland JL et al. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From anne-sophie.martinez <@t> unicaen.fr Tue Mar 21 02:15:42 2006 From: anne-sophie.martinez <@t> unicaen.fr (Anne-Sophie MARTINEZ) Date: Tue Mar 21 02:12:57 2006 Subject: [Histonet] (pas de sujet) Message-ID: <441FB62E.5070000@unicaen.fr> Many thanks for your answers! ASM From lpwenk <@t> sbcglobal.net Tue Mar 21 04:12:03 2006 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Tue Mar 21 04:13:14 2006 Subject: [Histonet] MVP II Message-ID: <001701c64ccf$e6b52410$7e31d445@HPPav2> Have an old MVP II tissue processor, desperately needing several alcohol/xylene containers. Leaking! So if anyone (including vendors) has some old containers laying around, I'm more than happy to pay for shipping. Sorry vendors, I'm run a school, and have no money to buy a new or used tisse processor. Would have a little money to buy the containers. Will also entertain suggestions on a type of "glue" I could use to plug the leaks, that resists alcohols and xylenes. That one's got me stumped! Thanks. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 From anne-sophie.martinez <@t> unicaen.fr Tue Mar 21 07:13:35 2006 From: anne-sophie.martinez <@t> unicaen.fr (Anne-Sophie MARTINEZ) Date: Tue Mar 21 07:10:50 2006 Subject: [Histonet] oysters larvae again Message-ID: <441FFBFF.2040707@unicaen.fr> Hello, (1) I plan to fix oysters larvae/juveniles (4 month) for in toto ISH. Which fixative is the best : Davidson (10% formaldehyde 37-40%, 30% ethanol 95%, 60% seawater, 10% acetic acid) or MEMPFA-T (0,1 M Mops pH 7,4; 2 mM EGTA; 1 mM MgSO4; 4% paraformaldehyde; 0,1% Tween 20)? (2) Would it be possible to use the MEMPFA-T to fix and "decalcify" the shelves of the juveniles for TEM (embedding in type lowyril, unicril, LR-white resins) instead of classical fixative (4% PFA) and then EDTA? Thank you very much again. Anne-Sophie From dpconsult <@t> earthlink.net Tue Mar 21 07:49:46 2006 From: dpconsult <@t> earthlink.net (Histonews) Date: Tue Mar 21 07:49:59 2006 Subject: [Histonet] Illinois Society for Histotechnologists - Annual Symposium May 18 & 19, 2006 Message-ID: Illinois Society for Histotechnologists Annual Symposium Convention May 18 & 19, 2006 Lisle Hilton, Lisle, IL Histotechs Searching for the Tree of Knowledge The Illinois Society for Histotechnologists invites you to join us for the Annual Symposium/Convention which will be held this year at the beautiful Hilton Lisle/Naperville on May 18 &19, 2006. Once again the 10 half-day workshops will be presented along with one half day of lectures. All workshops are approved for continuing education Hilton Lisle/Naperville 3003 Corporate West Drive Lisle, Illinois 630-505-0900 www.lislenaperville.hilton.com Room rates for the 2006 ISH Symposium are $99 -$109 Please call by April 15 and mention the ISH when making your reservation to receive this rate. [The program and registration is included in this message below] Any questions??? Eileen Dusek: e_dusek1@comcast.net (630) 527-3545 Judy Bordyn: jborydn@comcast.net Educational Programs Thursday May 18 7:00 - 8:00 Registration [Rolls, coffee and juice provided] Morning Lectures 8:00 - 8:15 President?s Welcome Jane Chladny 8:15 - 9:00 Cytology for Histologists Michelle Benruebin 9:00 - 10:15 Future of Histology Jim Robinson 10:15 - 10:45 Break with Vendors 10:45 - 11:15 Transmissible Spongiform Encephalopathies: A brief, Prospective from the Animal World 11:15 - 12:00 Histology Jeopardy Ray Ortiz 12:00 - 1:00 Lunch 1:00 - 4:30 Afternoon Workshops [ 2:30 - 3:30 Break with Vendors] #1 A Brave New World: An Introduction to Molecular Pathology. Dr. Thomas Haas #2 Dilutions, Titrations and then Some. Jim Chalmers #3 New Direction in the CAP Laboratory Accreditation Program Dr. Francis E. Sharkey Friday May 19 7:00 - 8:00 Registration 8:00 - 11:45 Morning Workshops [ 9:45 - 10:30 Break with Vendors] #4 DIY Basic Repair and Maintenance of the Equipment in Your Lab Matt Mincer and Stanley Weglarz #5 Florescence In Situ Hybridization (FISH), Theory and Application on Paraffin Embedded Tissue Yelina X. Noskina #6 DAKO The Continuum from Antibodies to Personalized Medicine Mary Cheles 11:45 - 1:00 Lunch & Awards Ceremony #7 Emerging Infectious Diseases and Zoonotic Agents; the new, the old and ugly Maureen Doran and Jane Chladny #8 How to Motivate your Team Sally Johnson #9 Histology Nuts and Bolts- Back to the Basics MariAnn Mailhoit WORKSHOP DETAILS #1 The last several decades have shown an increase in the role of genetics in a variety of laboratory testing methods. Diagnostic molecular pathology has become one of the fastest growing fields in pathology, and promises to continue to do so. This seminar will present basic genetic and molecular principles that will allow continued familiarity with ongoing rapid progress and advancement seen with this relatively new discipline. In Molecular Pathology, molecular biology methods are used to test DNA and/or RNA from patients specimens, diagnose disease, direct choice of therapy, detect residual/recurrent disease after therapy and provide prognostic information for the patient. The intent of this course will be to provide a foundation in molecular pathology laboratory. Certain diseases will be presented as case studies and illustration of methods used. Additionally, new areas of research and development in molecular pathology will be examined. #2. This is a presentation that takes the ?student? back to some basic laboratory practices. It will discuss what dilutions and titrations are and how, when and why to set them up in a lab. Basic pipette use, calibration and record keeping will be reviewed as well as different of pipettes. Formulas for primary dilutions and making them from a working stock will be discussed. Record keeping, ?Spec Sheet? and control tissue will also be presented. A cost comparison between concentrates and predilutes, showing the pros and cons for each. #3 This session will focus on changes to the CAP Laboratory Accreditation process and inspection checklists. Topics will include an overview of a new development in the accreditation program (e.g., unannounced inspections, new Team Leader Checklists, Inspector tools and inspection techniques, focus on patient safety, mandatory team leader training, and Anatomic Pathology Checklists changes to Histology). The participants? will understand the rationale behind these new developments #4 Let?s face it: Life in the lab is stressful enough without having to worry whether your equipment will work when you get there, Over the years we have found that many problems can be resolved quite easily and with no technical background. This workshop is designed to help the Histotech resolve many of these issues without having to ?call the service guys?. We will attempt to show you as many quick and easy fixes as we can. We will also discuss maintenance and some do? s and don?ts that will help prevent future problems. Hopefully you find this information helpful and use it to avoid potential needless service calls #5 Although FISH has been a technique in use sine 1984 it was not commonly available fro cytogenetic analysis until early 1990?s, with the use of FISH on paraffin embedded tissue sections first described in 1992. While the principals of FISH technology are straightforward, proper technique is important in obtaining accurate results. FISH technologies have become a cornerstone in genetic research and the development of personalized drug therapies. It is in the area of clinical assessment where FISH already provides valuable information to physicians. Proper technique for the use of FISH on paraffin sections, once established can be used for a myriad of applications. One such FISH assay is the determination of HER2 status currently provides valuable information regarding prognostic outcomes and predicts therapeutic value. Overall, FISH ,a powerful tool in the area of molecular and cytogenitic research, has expanded into the field of Histology, where future assays may progress to the level that HER2 FISH status holds today. #6 Dako Targeted-therapy. Predictive medicine. What does all this mean and where does the histology community fit in? The Analysis of a patient has historically relied on morphology and the evaluation of individual antibodies on pathological tissue. Therapeutic antibodies have become important strategy in the treatment of various solid tumors requiring involvement by pathology and laboratory medicine to meet the challenge. In the future, personalized medicine will define the effects of a therapy based on an individual?s genes and protein profile. In this workshop we will review this continuum leading us towards personalized medicine. #7 What are the risks of handling biohazards such as Tuberculosis, herpes B-virus and rabies. Discussion will include recommended procedures for bioterrorism agents such as tularemia and anthrax, also emerging diseases, like West Nile virus, SARS, avian flu and transmissible spongiform encephalopathies. Handling of these and other biohazards will be covered in this workshop. Exposure assessment, evaluation of risks and biosafety levels will be addressed, as well as containment, engineering controls, personal protective equipment and decontamination. Case histories of zoonotic exposure in laboratory workers will be used to predict future incident #8. How to motivate your team is a dynamic and emergent seminar based on research and practicality. It offers hands on strategy for communication and ideas to enhance component to enjoy your job and be successful and versatile. This seminar will also reveal the secrets on how to tap into your teams talents and arrive at great quality with an improved turn around time for your organization. #9.Ever wonder why we do things the way we do them? As a participant you will learn things like: What does formalin, alcohol , and xylene or xylene substitute do to the tissue during processing? What is the proper way to embed skin? Does it really matter how I turn the wheel on my microtome. H&E stains are yucky, where and how do I resolve the problem? How thin ins too thin and how thick is too thick. Whether you are a newcomer or an old schooler, a review of basic Histology is essential. INSTITUITIONAL PASS 2-Day Pass $300 _______ [Two techs from one institution may be present at any given time during both days of the meeting] (Maximum of 8 different people) Facility Information: ___________________________________________ ___________________________________________ ___________________________________________ Thursday May 18 Morning Session, LECTURE Tech #1_________________________________ Tech #2_________________________________ Afternoon Session, WORKSHOP Tech #1_________________________________ Tech #2_________________________________ Friday, May 19 Morning Session, WORKSHOP Tech #1_________________________________ Tech #2_________________________________ Afternoon Session, WORKSHOP Tech #1_________________________________ Tech #2_________________________________ 1 Day Pass $175_________ [Two Techs from one institution may be present at any given time on Thursday OR Friday] (Maximum of 4 different people) Facility Information __________________________________________________ __________________________________________________ ___________________________________________________ Please choose day techs will be attending: __________May 18 __________May 19 Morning Session List Lecture or Workshop Tech #1__________________________________________ Tech #2 _________________________________________ Afternoon Session, List Workshop Tech #1__________________________________________ Tech #2__________________________________________ 2006 ISH SYMPOSIUM / CONVENTION REGISTRATION FORM Please return by April15, 2006 $30_______ Registration Fee (non refundable)___Non Members [Includes ISH Membership] $10_______ Registration Fee (non refundable)___Members Thursday May 18 $45_______ Lecture Session (Morning) Afternoon Workshops (Choose 1) $45_______#1 Brave New World, Molecular Pathology $45_______#2 Dilution, Titration and Then Some $45_______#3 New Direction in the CAP Lab. Accreditation Friday May 19 Morning Workshops (Choose 1) $45_______#4 DIY Repair and Maintenance of Equipment $45_______#5 FISH on Paraffin Embedded Tissue $45_______#6 Continuum from Antibody to Personalized Medicine Afternoon Workshops (Choose 1) $45_______#7 Emerging Infectious Diseases $45_______#8 How to Motivate your Team $45_______#9 Histology Nuts and Bolts LUNCH IS INCLUDED BOTH DAYS TO ALL ATTENDEES Name________________________________________________________ Home Address: ________________________________________________ _________________________________________________ Home Phone_______________________ Work Address: _________________________________________________ _________________________________________________ Work Phone _______________________ Would you prefer information sent to home or work?_______________ eMail Address _____________________________________________________ I would prefer to be contacted primarily by email. YES_____NO______ All information will be used only for ISH membership purposes Please return by April 15, with check payable to: Illinois Society for Histotechnologists C/O Judy Bordyn 532 65th Street Downers Grove, Ill 60516 Any questions??? E-mail Judy at jborydn@comcast.net or Eileen Dusek at e_dusek1@comcast.net (630) 527-3545 From jstn192 <@t> yahoo.com Tue Mar 21 07:56:40 2006 From: jstn192 <@t> yahoo.com (Justin Thomas) Date: Tue Mar 21 07:56:44 2006 Subject: [Histonet] RE: Cheap Accessories Message-ID: <20060321135640.40293.qmail@web35712.mail.mud.yahoo.com> I am head of histology/pathology at a hospital in California, and I am putting the word out for members to respond....there is a company that can make identical microwave/any other accessories made of hard or soft material to accommodate equipment relatively economical! I have ordered a number of different accessories from this company, and they have great service. I think each member will be pleased with their services. Just reply to my email if you are interested....and i will give you their contact information ! -Thanks --------------------------------- Relax. Yahoo! Mail virus scanning helps detect nasty viruses! From ROrr <@t> enh.org Tue Mar 21 08:20:08 2006 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Tue Mar 21 08:20:16 2006 Subject: [Histonet] desperately seeking Chewie Message-ID: I'm looking for Chewie from Yuma Regional. I evidently wrote your email address wrong and can't get that information through to you. It keeps bouncing back Let me know! Bec Becky Orr CLA,HT(ASCP) IHC Lead Evanston Northwestern Healthcare 847-570-2771 From cwscouten <@t> myneurolab.com Tue Mar 21 08:43:45 2006 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Tue Mar 21 08:44:19 2006 Subject: [Histonet] Looking for Richard Thrift Message-ID: <5784D843593D874C93E9BADCB87342AB01306C41@tpiserver03.Coretech-holdings.com> I have an old email address for Richard Thrift and need to contact him. Anybody know where he is now? Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com From Thelda.Atkin <@t> US.ARMY.MIL Thu Mar 9 10:24:15 2006 From: Thelda.Atkin <@t> US.ARMY.MIL (Atkin, Thelda J LTC WRAIR Wash-DC) Date: Tue Mar 21 08:58:57 2006 Subject: [Histonet] Histotechnician Position announcement Walter Reed Army Institute of Research- Comparative Pathology Message-ID: DEPARTMENT OF THE ARMY Vacancy Announcement Number: NEBB06178220 Opening Date: March 10, 2006 Closing Date: March 20, 2006 Position: HISTOPATHOLOGY TECHNICIAN, DE-0646-3 Salary: $44,856 - $70,558 Annual Place of Work: WALTER REED ARMY INSTITUTE OF RESEARCH, DIVISION OF PATHOLOGY, DEPARTMENT OF COMPARATIVE PATHOLOGY, SILVER SPRING, MD Position Status: This is a Permanent position. -- Full Time Number of Vacancy: 01 Duties: Work involves knowledge of all histopathology procedures to ensure quality and promptness. Must provide expert supervision and technical skills to produce tissue samples suitable for microscopic examination by pathologists and other investigators. The work can be very tedious and requires significant patience to produce high quality stained specimens of 2-20 micron thickness. Candidate is expected to perform unusual techniques, such as frozen microtomy, immunohistochemistry and plastic embedding/microtomy. Work involves leading three or more employees in accomplishing their work. Must have the ability to assist a team through knowledge and application of leadership and team building skills and techniques such as group facilitation, coordination, coaching, problem solving, interpersonal communication, and integration of work processes and products. About the Position: This position is for a Lead Histotechnologist who will be responsible for all aspects of the operation and management of the Department of Comparative Pathologys Histology Laboratory. The lab produces an average of 16,000 slides and 10,000 paraffin blocks a year in support of the Walter Reed Army Institute of Research and Navy Research Medical Command experimental protocols and the Division of Veterinary Medicines quality assurance program. Who May Apply: (Click on Who May Apply) * Veterans eligible under Veterans Employment Opportunities Act of 1998. (VEOA) * Interagency Career Transition Assistance Plan (ICTAP) eligibles. * Defense Civilian Intelligence Personnel System (DCIPS) eligibles. * All Federal employees serving on a career or career-conditional appointment. * Department of Defense employees serving on a Career or Career Conditional Appointment. * Current Army employees with competitive status (includes Army employees serving on a career or career-conditional appointment). * Reinstatement eligibles. Qualifications: Click on link below to view qualification standard. General Schedule * 1. The Walter Reed Army Institute of Research is participating in an alternative personnel system known as the Personnel Demonstration Project. This position incorporates the GS-09 to GS-11 level. In keeping with the Demonstration pay fixing policies, employees already in the band will not receive a pay increase. 2. SPECIALIZED EXPERIENCE: Candidates for this position must show in their resume that they have one year of specialized experience and training that provided knowledge of histopathology procedures to ensure quality and promptness. This year of specialized experience must have included responsibility for performing unusual techniques, such as frozen microtomy, immunohistochemistry and plastic embedding / microtomy. * GS-06 and above: One year of experience directly related to this occupation equivalent to the next lower grade. Graduate education or an internship meets the experience required when it is directly related to the work of the position. * The experience described in your resume will be evaluated and screened for the Office of Personnel Management's basic qualifications requirements, and the skills needed to perform the duties of this position as described in this vacancy announcement. * Foreign education must be evaluated for U.S. equivalency in order to be considered for this position. Please include this information in your resume. * One year of experience in the same or similar work equivalent to at least the next lower grade or level requiring application of the knowledge, skills, and abilities of the position being filled. * Only degrees from an accredited college or university recognized by the Department of Education are acceptable to meet positive education requirements or to substitute education for experience. For additional information, please go to the Office of Personnel Management (OPM) and U.S. Department of Education websites at - http://www.opm.gov/qualifications and http://www.ed.gov/admins/finaid/accred/index.html Other Information: (Click on Other Information) * The Department of Defense (DoD) policy on employment of annuitants issued March 18, 2004 will be used in determining eligibility of annuitants. The DoD policy is available on http://www.cpms.osd.mil/fas/staffing/pdf/rem_ann.pdf * Permanent Change of Station (PCS) expenses are not authorized. * Temporary Duty (TDY) travel is 10 percent. Other Advantages: The Walter Reed Army Institute of Research is 10 minutes from Washington, D.C. If you would like more information about our installation, please visit our website at http://wrair-www.army.mil Other Requirements: (Click on Other Requirements) * Must be able to obtain and maintain a Secret security clearance. * A medical examination is required. * You will be required to provide proof of U.S. Citizenship. * Extended probationary period for the Engineers and Scientists Occupational Family will be three years. Extended probationary period for all other Occupational Families will be two years. * Male applicants born after December 31, 1959 must complete a Pre-Employment Certification Statement for Selective Service Registration. * Direct Deposit of Pay is Required. * This is a DOD Demonstration Project position. How to Apply: (Click on How to Apply) * Resumes must be received by the closing date of this announcement. * Self-nomination must be submitted by the closing date. * Resume must be on file in our centralized database. * Announcements close at 12:00am (midnight) Eastern Time. If your resume is currently in our central database, you may click here to Self Nominate Click here to use the Army Resume Builder to create your resume. Follow the instructions in this vacancy announcement to apply for the job. Point of Contact: Central Resume Processing Center, 410-306-0137, applicanthelp@cpsrxtp.belvoir.army.mil THE DEPARTMENT OF DEFENSE IS AN EQUAL OPPORTUNITY EMPLOYER From beth_zink <@t> yahoo.com Tue Mar 21 08:52:25 2006 From: beth_zink <@t> yahoo.com (beth zink) Date: Tue Mar 21 09:02:19 2006 Subject: [Histonet] hello Message-ID: <20060321145225.2646.qmail@web60123.mail.yahoo.com> Could you please send me directions on how to ask questions and get responses? Thanks Beth --------------------------------- Yahoo! Mail Bring photos to life! New PhotoMail makes sharing a breeze. From ploykasek <@t> phenopath.com Tue Mar 21 09:35:50 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Tue Mar 21 09:36:15 2006 Subject: [Histonet] Job posting Message-ID: I'm posting this job opening for a friend in research who is having trouble with her internet connection. This position is located north of Seattle. HISTOLOGIST MDS Pharma Services, Division of Efficacy-Pharmacology, is a therapeutically focused contract research organization that specializes in bone and central nervous system (CNS) biologies. We are currently seeking an experienced histologist to primarily participate in the Company?s pre-clinical bone research programs. Responsibilities will include hard tissue processing, embedding, thin sectioning, ground sections (using the EXAKT system), staining of both thin and ground sections, and the development and validation of laboratory techniques in support of client-sponsored research programs. Specific experience in orthopedic histological techniques is highly desired. Qualified applicants will possess a BS/MS degree and/or HT/HTL certification, 4+ years of related histology experience and documented expertise in hard tissue preparation and sectioning (usually MMA), and use of the EXAKT system. Candidates with prior experience working in a GLP compliant laboratory and expertise with histomorphometry will be considered superior. MDS Pharma Services offers an energetic and challenging research environment with a competitive compensation and benefits package including equity participation. Interested applicants can apply via the MDS Pharma Services website located at www.mdsps.com . The position can be found by searching for jobs in the United States, and location, Bothell-West. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From Charles.Embrey <@t> carle.com Tue Mar 21 09:38:51 2006 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Tue Mar 21 09:38:57 2006 Subject: [Histonet] hello Message-ID: You just did. That is all there is to it. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of beth zink Sent: Tuesday, March 21, 2006 8:52 AM To: histonet@pathology.swmed.edu Subject: [Histonet] hello Could you please send me directions on how to ask questions and get responses? Thanks Beth --------------------------------- Yahoo! Mail Bring photos to life! New PhotoMail makes sharing a breeze. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Tue Mar 21 10:23:04 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Tue Mar 21 10:23:16 2006 Subject: [Histonet] oysters larvae again Message-ID: <09C945920A6B654199F7A58A1D7D1FDE017176A4@lsexch.lsmaster.lifespan.org> I don't have a background in ISH, so I can't offer an opinion on the best fixative for that purpose. Regarding your second question, while I'm not familiar with either fixative, I can see that the Davidson fixative, containing 10% acetic acid and no buffers, would decalcify (dissolve) larval oyster shells. But the MEMPFA-T appears to be made up in pH 7.4 buffer, in which case I don't see how it would decalcify. In any case, I am wondering why you are using a formaldehyde-based fixative for TEM? Ordinarily a glutaraldehyde-based fixative would be preferable for TEM. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > Anne-Sophie MARTINEZ > Sent: Tuesday, March 21, 2006 5:13 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] oysters larvae again > > Hello, > (1) I plan to fix oysters larvae/juveniles (4 month) for in toto ISH. > Which fixative is the best : Davidson (10% formaldehyde 37-40%, 30% > ethanol 95%, 60% seawater, 10% acetic acid) or MEMPFA-T (0,1 M Mops pH > 7,4; 2 mM EGTA; 1 mM MgSO4; 4% paraformaldehyde; 0,1% Tween 20)? > (2) Would it be possible to use the MEMPFA-T to fix and "decalcify" the > shelves of the juveniles for TEM (embedding in type lowyril, unicril, > LR-white resins) instead of classical fixative (4% PFA) and then EDTA? > Thank you very much again. > Anne-Sophie > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From b003046 <@t> nf.au.dk Tue Mar 21 10:23:48 2006 From: b003046 <@t> nf.au.dk (b003046@nf.au.dk) Date: Tue Mar 21 10:23:55 2006 Subject: [Histonet] Lineage Negative Cells Message-ID: <1142958228.44202894e08a0@webmail.nf.au.dk> Hi, I am looking for a method to exclude lineage negative cells (Lin-) in rats in immunohistochemistry (paraffin-embedded tissue). Which antibodies should I use and is it possible to buy a detection kit? Any help would be appreciated. kind regards Mette K. Hagensen From plucas <@t> biopath.org Tue Mar 21 13:05:02 2006 From: plucas <@t> biopath.org (Paula Lucas) Date: Tue Mar 21 13:06:16 2006 Subject: [Histonet] Sakura Finetek Tissue-Tek Xpress Message-ID: <000201c64d1a$5c3e19e0$7c01a8c0@biopath.org> If anyone is using this processor, could you please give me your comments regarding the use of the instrument and the quality of the tissues? Thanks in advance. Paula Bio-Path Lab From jqb7 <@t> cdc.gov Tue Mar 21 13:16:44 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Tue Mar 21 13:19:24 2006 Subject: [Histonet] Sakura Finetek Tissue-Tek Xpress Message-ID: We have had ours since December 2005 and are very pleased with it. I will be happy to answer any specific questions you may have. Basically, it is extremely simple to use and the quality of the processed tissues and the subsequent staining has been superb. Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Paula Lucas Sent: Tuesday, March 21, 2006 2:05 PM To: Histonet (E-mail) Subject: [Histonet] Sakura Finetek Tissue-Tek Xpress If anyone is using this processor, could you please give me your comments regarding the use of the instrument and the quality of the tissues? Thanks in advance. Paula Bio-Path Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From glenn_krasinski <@t> yahoo.com Tue Mar 21 13:44:42 2006 From: glenn_krasinski <@t> yahoo.com (Glenn Krasinski) Date: Tue Mar 21 13:44:46 2006 Subject: [Histonet] H2DCFDA staining nuclear or perinuclear? Message-ID: <20060321194442.2169.qmail@web37502.mail.mud.yahoo.com> I have already bugged the flowers today with my H2DCFDA saga. Now this question is for my brothers/sisters on Histonet. We are noting staining with H2DCFDA to be nuclear and perinuclear in A431 cells both untreated and treated with compound. (and not super pretty I might add). In Li et al JBC Vol. 281, No.3, pp.1495-1505. There are some ultra pretty pictures showing it to be perinucluear at a similar concnetration (10uM H2DCFDA). Any ideas? Comments? Personal experiences? Many thanks. Glenn M. Krasinski Flow Jock in Retirement San Diego, CA --------------------------------- Yahoo! Travel Find great deals to the top 10 hottest destinations! From sbreeden <@t> nmda.nmsu.edu Tue Mar 21 14:08:47 2006 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Mar 21 14:08:53 2006 Subject: [Histonet] NM SOCIETY FOR HISTOLOGY Message-ID: Reading these postings about other State meetings reminds me that I need to post a notice about New Mexico. Although it was hoped to have an Annual Conference in June of 2006, the fact that the NMSH has been "dormant" for over two years has made a meeting this year impractical. We'll continue to try to "rejuvenate" and hope to be able to plan a meeting in the near future. Vendors, please take note and accept my apologies for not posting this earlier; the decision was made in the very recent past. Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services POBox 4700 Albuquerque, NM 87196 (505)841-2576 (505)841-2518 FAX From hwang78 <@t> gmail.com Tue Mar 21 14:19:21 2006 From: hwang78 <@t> gmail.com (mickywang) Date: Tue Mar 21 14:19:29 2006 Subject: [Histonet] urgent for second antibody Message-ID: Hi all, I always use vector's kits to work IHC and they are fine.... I just found my biotinylated anti-mouse IgG reagent is done but I still have other reagents. My question is if I can use the second antibody from DAKO LSAB+ kit to replace vector's second antibody? Any suggestion is highly appreciated. Thanks From gpbnas <@t> yahoo.es Tue Mar 21 14:33:39 2006 From: gpbnas <@t> yahoo.es (Guillermo Palao) Date: Tue Mar 21 14:33:45 2006 Subject: [Histonet] urgent for second antibody In-Reply-To: Message-ID: <20060321203339.8449.qmail@web26211.mail.ukl.yahoo.com> I use Jackson's secondary-biotinylated antibodies with Vector ABC. In mouse kidney samples the Jackson mouse pre-adsorbed donkey antirabbit detected rabbit primary without crossreacting with deposited mouse IgG, whereas Vector goat anti-rabbit did cross-react. Hope this helps, Guillermo mickywang escribi?: Hi all, I always use vector's kits to work IHC and they are fine.... I just found my biotinylated anti-mouse IgG reagent is done but I still have other reagents. My question is if I can use the second antibody from DAKO LSAB+ kit to replace vector's second antibody? Any suggestion is highly appreciated. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- LLama Gratis a cualquier PC del Mundo. Llamadas a fijos y m?viles desde 1 c?ntimo por minuto. http://es.voice.yahoo.com From Charlotte.Kopczynski <@t> baycare.org Tue Mar 21 14:38:05 2006 From: Charlotte.Kopczynski <@t> baycare.org (Kopczynski, Charlotte) Date: Tue Mar 21 14:38:01 2006 Subject: [Histonet] Job Openings Message-ID: <5452D66669CFC540B0452115ED26AF1F040E5810@bcexch02.bcad.baycare.org> Baycare Health System in the Tampa Bay area has Histology Openings for the following positions: Mease Hospital in Dunedin, Fl. These positions will start at Mease Hospital with transition to Morton Plant Hospital when Histology processing is consolidated for Morton Plant Mease Health Care Histologist Midnight - 8:30 AM Histologist 6:30 AM - 3:00 PM St. Joseph's Hospital in Tampa, Fl. Lead Histologist 10:00 PM - 6:30 AM PRN Histologist - varied hours St. Anthony's Hospital in St. Petersburg, Fl. Histologist - Fulltime Flexible Hours Lead Histologist - 7:30 AM - 4:00 PM Thanks, Charlotte Kopczynski,HTL(ASCP) Baycare Pathology Manager Phone: 727-461-8246 Fax: 727-462-7597 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 28, Issue 33 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: lectin stains and antigen retrival (Gayle Callis) 2. RE: TEM and oysters (Monfils, Paul) 3. Re: mucicarmine (Johnson, Teri) 4. 2006 Missouri Symposium - June 2-3 St. Louis, MO (Johnson, Teri) 5. North Carolina Spring Mtg. (Delorise Williams) 6. RE: Re: mucicarmine (Kathy.Johnston@CLS.ab.ca) 7. Human CD31, CD34, von Willebrands etc publication (Gayle Callis) 8. Rat CD8 marker attn: Patsy Ruegg (Gayle Callis) 9. (pas de sujet) (Anne-Sophie MARTINEZ) 10. MVP II (Lee & Peggy Wenk) 11. oysters larvae again (Anne-Sophie MARTINEZ) 12. Illinois Society for Histotechnologists - Annual Symposium May 18 & 19, 2006 (Histonews) 13. RE: Cheap Accessories (Justin Thomas) 14. desperately seeking Chewie (Orr, Rebecca) 15. Looking for Richard Thrift (Charles Scouten) 16. Histotechnician Position announcement Walter Reed Army Institute of Research- Comparative Pathology (Atkin, Thelda J LTC WRAIR Wash-DC) 17. hello (beth zink) 18. Job posting (Patti Loykasek) 19. RE: hello (Charles.Embrey) 20. RE: oysters larvae again (Monfils, Paul) 21. Lineage Negative Cells (b003046@nf.au.dk) ---------------------------------------------------------------------- Message: 1 Date: Mon, 20 Mar 2006 11:11:18 -0700 From: Gayle Callis Subject: Re: [Histonet] lectin stains and antigen retrival To: Franz-Josef M?ller , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20060320105732.01b79d30@gemini.msu.montana.edu> Content-Type: text/plain; charset="iso-8859-1"; format=flowed Yes, lectins are stable although trypsin enzyme digestion at 37C may be better than citrate buffer retrieval. Also, there are buffer considerations for staining, i.e. TRIS Lectin buffer with Mg and Ca added. The book gives the recipe for this and with some lectins PBS should be avoided and also normal serums. AND there is an excellent book on Lectin Histochemistry , a concise practical handbook SA Brooks et al, ISBN#1859961002 that gives IHC staining methods, etc. It is not expensive and Springer Verlag is the publisher source. 01:18 AM 3/19/2006, you wrote: >Dear Histonetters, >does anybody know if lectin stains are stable after the tissue >section (20u, paraffin embedded) was subjected to heat antigen >retrieval in citric acid pH 6.0 ? >Thanks for your help in advance! >Cheers >franzef > >Dr. med. Franz-Josef M?ller >Zentrum f?r Integrative Psychiatrie, Kiel >Zellbiologisches Labor >Niemannsweg 147 >24105 Kiel >GERMANY > >Tel.: +49 431 597 4169 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) ------------------------------ Message: 2 Date: Mon, 20 Mar 2006 13:24:09 -0500 From: "Monfils, Paul" Subject: RE: [Histonet] TEM and oysters To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <09C945920A6B654199F7A58A1D7D1FDE017176A2@lsexch.lsmaster.lifespan.org> Content-Type: text/plain; charset="iso-8859-1" (1) For most types of antigens, not likely. Glutaraldehyde crosslinks proteins so aggressively that most antigenicity is greatly reduced or completely destroyed. If immunolabeling is the objective before the fact, the tissue should be fixed very conservatively, for example 0.25% glutaraldehyde for a half hour to one hour. Following normal glutaraldehyde fixation with osmium introduces additional problems, as osmium greatly denatures proteins, even those crosslinked by glutaraldehyde, and actually dissolves some pre-fixed proteins out of the tissue by breaking the glutaraldehyde bonds. The only successful attempt at immunolabeling glut/osmium fixed tissue that I can recall was a paper written by David Knibbs around 1992-1994 in which he described a method for immunolabeling (using colloidal gold-labeled antibodies) various kinds of intermediate filaments. I believe the abstract of that paper would be in the proceedings of the American Society of Cell Biology. (2) You cannot "decalcify" mollusc shells in the same sense that you can decalify bone because bone consists of a solid tissue matrix infused with calcium salts, while a molluscan shell is composed almost completely of calcium salts deposited by the animal's mantle, with only intermittent traces of protein. So, if you expose the shell to decalcifying agents, the shell will dissolve completely. If the loss of the shell is not a problem, then it can be dissolved away much more rapidly than a bone of equivalent size can be decalcified, since the bone matrix presents a barrier to the penetration of the decalcifying solution. When I taught chemistry years ago, I did a demo by filling a 1000 ml glass cylinder with 10% hydrochloric acid and dropping in a clam shell. A dense cloud of bubbles would immediately erupt, causing the liquid to "boil" as the calcium carbonate of the shell was transformed into carbon dioxide, and the shell would be completely gone in about two minutes. So, theoretically any decalcifying method could be used to remove the shell from a molluscan specimen. If you consider acid methods too harsh, I would recommend a chelation method such as disodium EDTA. I never tried this on a mollusc shell, but I see no reason why it wouldn't work. You'll have to experiment to find an optimum exposure time, but again it should be much shorter than the time required for decalcifying bone. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > Anne-Sophie MARTINEZ > Sent: Monday, March 20, 2006 7:55 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] TEM and oysters > > Hi everybody. I have 2 questions concerning TEM. > (1) I have fixed gonads of adult oysters for TEM with glutaraldehyde, > post-fixed with osmium and done the embedding in Epon. Is there any way > I can you do immunocytochemistry with antibodies on these samples? > (2) How can I decalcify the shelves of larvae and juveniles oysters for > TEM without damaging the tissue? > Could anyone help me please? Thanks for your suggestions. > Anne-Sophie > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 3 Date: Mon, 20 Mar 2006 12:47:51 -0600 From: "Johnson, Teri" Subject: [Histonet] Re: mucicarmine To: Message-ID: Content-Type: text/plain; charset="us-ascii" Dorothy, It's been a while since I've done one, but I remember we got our best results using Weigert's Iron Hematoxylin staining first, and then using Sigma's mucicarmine stain. I don't quite remember if we used it undiluted, but I think we did use it more concentrated than their protocol called for. Where I trained, we made up the mucicarmine stain and used undiluted stock to get brighter pink/rose staining results. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64133 ------------------------------ Message: 4 Date: Mon, 20 Mar 2006 12:58:05 -0600 From: "Johnson, Teri" Subject: [Histonet] 2006 Missouri Symposium - June 2-3 St. Louis, MO To: Message-ID: Content-Type: text/plain; charset="us-ascii" If anyone is interested in coming to our fantastic meeting and you need the registration forms and other information, please contact: Sharon Walsh (MO Society President): userwalsh@aol.com Teri Johnson (Exhibit coordinator): tjj@stowers-institute.org Rosetta Barkley (Registrar): rbarkley2@kumc.edu Our deepest gratitude is extended to all the exhibitors, sponsors and speakers that make the MSH meeting possible. MISSOURI SOCIETY FOR HISTOTECHNOLOGY Presents Meet me in St. Louis The 29th Annual Spring Symposium For Continuing Education and Histopathologic Technique June 2-3, 2006 Embassy Suites Hotel-St. Louis Downtown GENERAL INFORMATION Date: June 2-3, 2006 Meeting Location: Embassy Suites Hotel-St. Louis Downtown 901 North 1st Street St. Louis, Missouri 63102 314-241-4200 www.stlouisdowntown.embassysuites.com Room Reservations should be made directly with the Embassy Suites, St. Louis, MO. Hotel reservations deadline is May 2, 2006. To secure the symposium rates, make your reservations early. The Chicago Cubs and the Cardinal's are playing this weekend in St. Louis, no rooms will be held after May 2nd, and it will be almost impossible to get a room in St. Louis. Make your reservations early. Room Rates: $119.00 Thursday (6/1) and Friday (6/2) Each suite is beautifully decorated with a private bedroom and spacious living room. All of our suites are fully equipped with two televisions, a refrigerator, microwave oven, coffee maker, two telephones with data ports and a well lit dining/work table. The lobby atrium provides guests a complimentary full cooked-to-order breakfast and an Evening Managers Reception including your favorite beverages every evening. (Free food and Drink) Attractions include the President Casino and the St.Louis Arch. SCHEDULE Friday, June 2, 2006 7:30 - 8:00 REGISTRATION 8:15 - 8:30 President Message- Sharon Ann Walsh 8:30 - Noon SCIENTIFIC SESSIONS 8:30 - 9:15 Reagent Alcohol, What's it all about? Pamela Marcum, University of PA, School of Veterinary Medicine, Kennett Square, PA (Sponsored by Poly Scientific) 9:15 - 10:00 Importance of Special Stains in Renal Pathology Cherese Cortese, M.D., Associate Professor, Department of Pathology St. Louis University, St. Louis, Missouri 10:00 - 11:00 COFFEE BREAK & EXHIBITS 11:00 - 11:45 EM for the Histotechnologist Randy D. Tindall, EM Specialist, Electron Microscopy Core Facility, University of Missouri, Columbia 11:45 - 1:00 AWARDS LUNCHEON: Region V Update, Konnie Zeitner WORKSHOPS 1:30 - 4:30 3:00 - 3:30 COFFEE BREAK & EXHIBITS Workshop #1 Double Immuno Staining Wet Workshop, Tara Kennedy, Applications Specialist, Biocare Medical Workshop #2 Histology Nuts and Bolts. Back to the Basics, Mari Ann Mailhiot BA HT(ASCP), Specimen Applications Specialist, Leica Microsystems Workshop #3 Stars of the Silver Screen, Trouble shooting Silver Stains, Joan M. Vesey HT(ASCP), Richard-Allan Scientific 5:30-7:30 P.M. Upper Garden Atrium, Embassy Suites Pizza Party Saturday, June 3, 2006 7:30 - 8:00 REGISTRATION 8:00 - 8:15 Saturday Welcome- Sharon Ann Walsh, President MSH 8:30 - 11:30 SCIENTIFIC SESSIONS 8:30 - 9:30 Basics for using Pro-EX-C in the Detection of High Grade SC from Atypical Squamous Metaplasia Lourdes Ylagen, Department of Pathology and Immunology, Division of Surgical Pathology, Washington University School of Medicine, St. Louis, Missouri 9:30 - 10:30 Safety in the Laboratory, What we take for granted. Sylvia Casey 10:30-11:00 MSH Business Meeting (The meeting will be held in the general session room immediately following the scientific session. All are welcome to attend). 11:00 - 12:30 Exhibit Hall Open. (Ballpark Break) WORKSHOPS 1:00 - 4:00 Workshop #4 Preparing for a CAP Inspection, Charlie Dorner, Vision Biosystems Workshop #5 Histologic Techniques in Hematopathology, Clinical Applications and Troubleshooting, Sharad Mathur, M.D., VAMC, Kansas City, Stacey Merica, HT(ASCP), VAMC, Kansas City Workshop #6 Is your Processor Fighting You, Fight Back! Pamela Marcum, University of PA, School of Veterinary Medicine, Kennett Square, PA (Sponsored by Poly Scientific) All workshops are CEU approved. Advance Registration must be postmarked by May 27th, 2006 (no refunds after this date). ------------------------------ Message: 5 Date: Mon, 20 Mar 2006 15:08:30 -0500 From: "Delorise Williams" Subject: [Histonet] North Carolina Spring Mtg. To: Cc: Delorise Williams , david Weil , Lisa Gates , joanna.c.barton@gsk.com, Wanda Jones Message-ID: <12816CB59E68184F9F5F28063E68B04702A60D85@xsrvr.ciit.org> Content-Type: text/plain; charset="us-ascii" The North Carolina Society of Histopathology Technologists will be hosting their annual spring meeting at the Greensboro/ Highpoint Airport Marriott in Greensboro, NC. on April 21-22, 2006. We welcome you to come and experience North Carolina's beautiful springtime scenery. We have a very interesting program planned with such presenters as Peggy Wenk, Robert Lott, Lamar & Wanda Jones, Connie Wavrin and John Wilson from Ventana. For more info, contact me at dwilliams@ciit.org Delorise Williams CIIT Centers for Health Research PO Box 12137 Research Triangle Park, NC 27709 (919) 558-1200 Voice Mail-(919) 558-1252 Fax-(919) 558-1300 ------------------------------ Message: 6 Date: Mon, 20 Mar 2006 14:25:30 -0700 From: Subject: RE: [Histonet] Re: mucicarmine To: Message-ID: <4BC300747AF87A48BCDF8E48BC2885CE013652F2@mail1.calgary.com> Content-Type: text/plain; charset="iso-8859-1" We use the same stock here (Sigma) and dilute it 1:3 with dH2O. We do use Harris hematoxylin instead though. I have heard that you can use a tartrazine counterstain to emphasize the mucin staining if that is something your pathologists prefer. Kathy Johnston Tech II Special Stains Anatomic Pathology- DSC Calgary Laboratory Services 770-3572 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Johnson, Teri Sent: March 20, 2006 11:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: mucicarmine Dorothy, It's been a while since I've done one, but I remember we got our best results using Weigert's Iron Hematoxylin staining first, and then using Sigma's mucicarmine stain. I don't quite remember if we used it undiluted, but I think we did use it more concentrated than their protocol called for. Where I trained, we made up the mucicarmine stain and used undiluted stock to get brighter pink/rose staining results. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64133 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify the sender immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. ------------------------------ Message: 7 Date: Mon, 20 Mar 2006 14:28:46 -0700 From: Gayle Callis Subject: [Histonet] Human CD31, CD34, von Willebrands etc publication To: Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20060320140932.01b0a7a8@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed There has been a great deal of discussion about some of these markers and this publication in J Histochemistry Cytochemistry just came out. If you need a copy, I will happy to forward a pdf of the article privately. Immunohistochemical Expression of Endothelial Markers CD31, CD34, von Willebrand Factor, and Fli-1 in Normal Human Tissues Marc P. Pusztaszeri, Walter Seelentag and Fred T. Bosman Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) ------------------------------ Message: 8 Date: Mon, 20 Mar 2006 15:19:22 -0700 From: Gayle Callis Subject: [Histonet] Rat CD8 marker attn: Patsy Ruegg To: Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20060320151602.01b0b040@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Patsy, There is an excellent article (even though from 1995) on Rat CD8 (OX 8) along with other rat CD markers and murine CD markers. The authors used PLP as the fixative for paraffin embedded tissue. Immunohistochemical detection of T cell subsets and other leukocytes in paraffin embedded rat and mouse tissues with monoclonal antibodies. J Histochem Cytochem 45(3):313-320,1995 Whiteland JL et al. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) ------------------------------ Message: 9 Date: Tue, 21 Mar 2006 09:15:42 +0100 From: Anne-Sophie MARTINEZ Subject: [Histonet] (pas de sujet) To: histonet@lists.utsouthwestern.edu Message-ID: <441FB62E.5070000@unicaen.fr> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Many thanks for your answers! ASM ------------------------------ Message: 10 Date: Tue, 21 Mar 2006 05:12:03 -0500 From: "Lee & Peggy Wenk" Subject: [Histonet] MVP II To: "'Histonet'" Message-ID: <001701c64ccf$e6b52410$7e31d445@HPPav2> Content-Type: text/plain; charset="us-ascii" Have an old MVP II tissue processor, desperately needing several alcohol/xylene containers. Leaking! So if anyone (including vendors) has some old containers laying around, I'm more than happy to pay for shipping. Sorry vendors, I'm run a school, and have no money to buy a new or used tisse processor. Would have a little money to buy the containers. Will also entertain suggestions on a type of "glue" I could use to plug the leaks, that resists alcohols and xylenes. That one's got me stumped! Thanks. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 ------------------------------ Message: 11 Date: Tue, 21 Mar 2006 14:13:35 +0100 From: Anne-Sophie MARTINEZ Subject: [Histonet] oysters larvae again To: histonet@lists.utsouthwestern.edu Message-ID: <441FFBFF.2040707@unicaen.fr> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hello, (1) I plan to fix oysters larvae/juveniles (4 month) for in toto ISH. Which fixative is the best : Davidson (10% formaldehyde 37-40%, 30% ethanol 95%, 60% seawater, 10% acetic acid) or MEMPFA-T (0,1 M Mops pH 7,4; 2 mM EGTA; 1 mM MgSO4; 4% paraformaldehyde; 0,1% Tween 20)? (2) Would it be possible to use the MEMPFA-T to fix and "decalcify" the shelves of the juveniles for TEM (embedding in type lowyril, unicril, LR-white resins) instead of classical fixative (4% PFA) and then EDTA? Thank you very much again. Anne-Sophie ------------------------------ Message: 12 Date: Tue, 21 Mar 2006 07:49:46 -0600 From: "Histonews" Subject: [Histonet] Illinois Society for Histotechnologists - Annual Symposium May 18 & 19, 2006 To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Illinois Society for Histotechnologists Annual Symposium Convention May 18 & 19, 2006 Lisle Hilton, Lisle, IL Histotechs Searching for the Tree of Knowledge The Illinois Society for Histotechnologists invites you to join us for the Annual Symposium/Convention which will be held this year at the beautiful Hilton Lisle/Naperville on May 18 &19, 2006. Once again the 10 half-day workshops will be presented along with one half day of lectures. All workshops are approved for continuing education Hilton Lisle/Naperville 3003 Corporate West Drive Lisle, Illinois 630-505-0900 www.lislenaperville.hilton.com Room rates for the 2006 ISH Symposium are $99 -$109 Please call by April 15 and mention the ISH when making your reservation to receive this rate. [The program and registration is included in this message below] Any questions??? Eileen Dusek: e_dusek1@comcast.net (630) 527-3545 Judy Bordyn: jborydn@comcast.net Educational Programs Thursday May 18 7:00 - 8:00 Registration [Rolls, coffee and juice provided] Morning Lectures 8:00 - 8:15 President's Welcome Jane Chladny 8:15 - 9:00 Cytology for Histologists Michelle Benruebin 9:00 - 10:15 Future of Histology Jim Robinson 10:15 - 10:45 Break with Vendors 10:45 - 11:15 Transmissible Spongiform Encephalopathies: A brief, Prospective from the Animal World 11:15 - 12:00 Histology Jeopardy Ray Ortiz 12:00 - 1:00 Lunch 1:00 - 4:30 Afternoon Workshops [ 2:30 - 3:30 Break with Vendors] #1 A Brave New World: An Introduction to Molecular Pathology. Dr. Thomas Haas #2 Dilutions, Titrations and then Some. Jim Chalmers #3 New Direction in the CAP Laboratory Accreditation Program Dr. Francis E. Sharkey Friday May 19 7:00 - 8:00 Registration 8:00 - 11:45 Morning Workshops [ 9:45 - 10:30 Break with Vendors] #4 DIY Basic Repair and Maintenance of the Equipment in Your Lab Matt Mincer and Stanley Weglarz #5 Florescence In Situ Hybridization (FISH), Theory and Application on Paraffin Embedded Tissue Yelina X. Noskina #6 DAKO The Continuum from Antibodies to Personalized Medicine Mary Cheles 11:45 - 1:00 Lunch & Awards Ceremony #7 Emerging Infectious Diseases and Zoonotic Agents; the new, the old and ugly Maureen Doran and Jane Chladny #8 How to Motivate your Team Sally Johnson #9 Histology Nuts and Bolts- Back to the Basics MariAnn Mailhoit WORKSHOP DETAILS #1 The last several decades have shown an increase in the role of genetics in a variety of laboratory testing methods. Diagnostic molecular pathology has become one of the fastest growing fields in pathology, and promises to continue to do so. This seminar will present basic genetic and molecular principles that will allow continued familiarity with ongoing rapid progress and advancement seen with this relatively new discipline. In Molecular Pathology, molecular biology methods are used to test DNA and/or RNA from patients specimens, diagnose disease, direct choice of therapy, detect residual/recurrent disease after therapy and provide prognostic information for the patient. The intent of this course will be to provide a foundation in molecular pathology laboratory. Certain diseases will be presented as case studies and illustration of methods used. Additionally, new areas of research and development in molecular pathology will be examined. #2. This is a presentation that takes the "student" back to some basic laboratory practices. It will discuss what dilutions and titrations are and how, when and why to set them up in a lab. Basic pipette use, calibration and record keeping will be reviewed as well as different of pipettes. Formulas for primary dilutions and making them from a working stock will be discussed. Record keeping, "Spec Sheet" and control tissue will also be presented. A cost comparison between concentrates and predilutes, showing the pros and cons for each. #3 This session will focus on changes to the CAP Laboratory Accreditation process and inspection checklists. Topics will include an overview of a new development in the accreditation program (e.g., unannounced inspections, new Team Leader Checklists, Inspector tools and inspection techniques, focus on patient safety, mandatory team leader training, and Anatomic Pathology Checklists changes to Histology). The participants' will understand the rationale behind these new developments #4 Let's face it: Life in the lab is stressful enough without having to worry whether your equipment will work when you get there, Over the years we have found that many problems can be resolved quite easily and with no technical background. This workshop is designed to help the Histotech resolve many of these issues without having to "call the service guys". We will attempt to show you as many quick and easy fixes as we can. We will also discuss maintenance and some do' s and don'ts that will help prevent future problems. Hopefully you find this information helpful and use it to avoid potential needless service calls #5 Although FISH has been a technique in use sine 1984 it was not commonly available fro cytogenetic analysis until early 1990's, with the use of FISH on paraffin embedded tissue sections first described in 1992. While the principals of FISH technology are straightforward, proper technique is important in obtaining accurate results. FISH technologies have become a cornerstone in genetic research and the development of personalized drug therapies. It is in the area of clinical assessment where FISH already provides valuable information to physicians. Proper technique for the use of FISH on paraffin sections, once established can be used for a myriad of applications. One such FISH assay is the determination of HER2 status currently provides valuable information regarding prognostic outcomes and predicts therapeutic value. Overall, FISH ,a powerful tool in the area of molecular and cytogenitic research, has expanded into the field of Histology, where future assays may progress to the level that HER2 FISH status holds today. #6 Dako Targeted-therapy. Predictive medicine. What does all this mean and where does the histology community fit in? The Analysis of a patient has historically relied on morphology and the evaluation of individual antibodies on pathological tissue. Therapeutic antibodies have become important strategy in the treatment of various solid tumors requiring involvement by pathology and laboratory medicine to meet the challenge. In the future, personalized medicine will define the effects of a therapy based on an individual's genes and protein profile. In this workshop we will review this continuum leading us towards personalized medicine. #7 What are the risks of handling biohazards such as Tuberculosis, herpes B-virus and rabies. Discussion will include recommended procedures for bioterrorism agents such as tularemia and anthrax, also emerging diseases, like West Nile virus, SARS, avian flu and transmissible spongiform encephalopathies. Handling of these and other biohazards will be covered in this workshop. Exposure assessment, evaluation of risks and biosafety levels will be addressed, as well as containment, engineering controls, personal protective equipment and decontamination. Case histories of zoonotic exposure in laboratory workers will be used to predict future incident #8. How to motivate your team is a dynamic and emergent seminar based on research and practicality. It offers hands on strategy for communication and ideas to enhance component to enjoy your job and be successful and versatile. This seminar will also reveal the secrets on how to tap into your teams talents and arrive at great quality with an improved turn around time for your organization. #9.Ever wonder why we do things the way we do them? As a participant you will learn things like: What does formalin, alcohol , and xylene or xylene substitute do to the tissue during processing? What is the proper way to embed skin? Does it really matter how I turn the wheel on my microtome. H&E stains are yucky, where and how do I resolve the problem? How thin ins too thin and how thick is too thick. Whether you are a newcomer or an old schooler, a review of basic Histology is essential. INSTITUITIONAL PASS 2-Day Pass $300 _______ [Two techs from one institution may be present at any given time during both days of the meeting] (Maximum of 8 different people) Facility Information: ___________________________________________ ___________________________________________ ___________________________________________ Thursday May 18 Morning Session, LECTURE Tech #1_________________________________ Tech #2_________________________________ Afternoon Session, WORKSHOP Tech #1_________________________________ Tech #2_________________________________ Friday, May 19 Morning Session, WORKSHOP Tech #1_________________________________ Tech #2_________________________________ Afternoon Session, WORKSHOP Tech #1_________________________________ Tech #2_________________________________ 1 Day Pass $175_________ [Two Techs from one institution may be present at any given time on Thursday OR Friday] (Maximum of 4 different people) Facility Information __________________________________________________ __________________________________________________ ___________________________________________________ Please choose day techs will be attending: __________May 18 __________May 19 Morning Session List Lecture or Workshop Tech #1__________________________________________ Tech #2 _________________________________________ Afternoon Session, List Workshop Tech #1__________________________________________ Tech #2__________________________________________ 2006 ISH SYMPOSIUM / CONVENTION REGISTRATION FORM Please return by April15, 2006 $30_______ Registration Fee (non refundable)___Non Members [Includes ISH Membership] $10_______ Registration Fee (non refundable)___Members Thursday May 18 $45_______ Lecture Session (Morning) Afternoon Workshops (Choose 1) $45_______#1 Brave New World, Molecular Pathology $45_______#2 Dilution, Titration and Then Some $45_______#3 New Direction in the CAP Lab. Accreditation Friday May 19 Morning Workshops (Choose 1) $45_______#4 DIY Repair and Maintenance of Equipment $45_______#5 FISH on Paraffin Embedded Tissue $45_______#6 Continuum from Antibody to Personalized Medicine Afternoon Workshops (Choose 1) $45_______#7 Emerging Infectious Diseases $45_______#8 How to Motivate your Team $45_______#9 Histology Nuts and Bolts LUNCH IS INCLUDED BOTH DAYS TO ALL ATTENDEES Name________________________________________________________ Home Address: ________________________________________________ _________________________________________________ Home Phone_______________________ Work Address: _________________________________________________ _________________________________________________ Work Phone _______________________ Would you prefer information sent to home or work?_______________ eMail Address _____________________________________________________ I would prefer to be contacted primarily by email. YES_____NO______ All information will be used only for ISH membership purposes Please return by April 15, with check payable to: Illinois Society for Histotechnologists C/O Judy Bordyn 532 65th Street Downers Grove, Ill 60516 Any questions??? E-mail Judy at jborydn@comcast.net or Eileen Dusek at e_dusek1@comcast.net (630) 527-3545 ------------------------------ Message: 13 Date: Tue, 21 Mar 2006 05:56:40 -0800 (PST) From: Justin Thomas Subject: [Histonet] RE: Cheap Accessories To: histonet@lists.utsouthwestern.edu Message-ID: <20060321135640.40293.qmail@web35712.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I am head of histology/pathology at a hospital in California, and I am putting the word out for members to respond....there is a company that can make identical microwave/any other accessories made of hard or soft material to accommodate equipment relatively economical! I have ordered a number of different accessories from this company, and they have great service. I think each member will be pleased with their services. Just reply to my email if you are interested....and i will give you their contact information ! -Thanks --------------------------------- Relax. Yahoo! Mail virus scanning helps detect nasty viruses! ------------------------------ Message: 14 Date: Tue, 21 Mar 2006 08:20:08 -0600 From: "Orr, Rebecca" Subject: [Histonet] desperately seeking Chewie To: Message-ID: Content-Type: text/plain; charset="US-ASCII" I'm looking for Chewie from Yuma Regional. I evidently wrote your email address wrong and can't get that information through to you. It keeps bouncing back Let me know! Bec Becky Orr CLA,HT(ASCP) IHC Lead Evanston Northwestern Healthcare 847-570-2771 ------------------------------ Message: 15 Date: Tue, 21 Mar 2006 08:43:45 -0600 From: "Charles Scouten" Subject: [Histonet] Looking for Richard Thrift To: Message-ID: <5784D843593D874C93E9BADCB87342AB01306C41@tpiserver03.Coretech-holdings.com> Content-Type: text/plain; charset="us-ascii" I have an old email address for Richard Thrift and need to contact him. Anybody know where he is now? Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com ------------------------------ Message: 16 Date: Thu, 9 Mar 2006 11:24:15 -0500 From: "Atkin, Thelda J LTC WRAIR Wash-DC" Subject: [Histonet] Histotechnician Position announcement Walter Reed Army Institute of Research- Comparative Pathology To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" DEPARTMENT OF THE ARMY Vacancy Announcement Number: NEBB06178220 Opening Date: March 10, 2006 Closing Date: March 20, 2006 Position: HISTOPATHOLOGY TECHNICIAN, DE-0646-3 Salary: $44,856 - $70,558 Annual Place of Work: WALTER REED ARMY INSTITUTE OF RESEARCH, DIVISION OF PATHOLOGY, DEPARTMENT OF COMPARATIVE PATHOLOGY, SILVER SPRING, MD Position Status: This is a Permanent position. -- Full Time Number of Vacancy: 01 Duties: Work involves knowledge of all histopathology procedures to ensure quality and promptness. Must provide expert supervision and technical skills to produce tissue samples suitable for microscopic examination by pathologists and other investigators. The work can be very tedious and requires significant patience to produce high quality stained specimens of 2-20 micron thickness. Candidate is expected to perform unusual techniques, such as frozen microtomy, immunohistochemistry and plastic embedding/microtomy. Work involves leading three or more employees in accomplishing their work. Must have the ability to assist a team through knowledge and application of leadership and team building skills and techniques such as group facilitation, coordination, coaching, problem solving, interpersonal communication, and integration of work processes and products. About the Position: This position is for a Lead Histotechnologist who will be responsible for all aspects of the operation and management of the Department of Comparative Pathologys Histology Laboratory. The lab produces an average of 16,000 slides and 10,000 paraffin blocks a year in support of the Walter Reed Army Institute of Research and Navy Research Medical Command experimental protocols and the Division of Veterinary Medicines quality assurance program. Who May Apply: (Click on Who May Apply) * Veterans eligible under Veterans Employment Opportunities Act of 1998. (VEOA) * Interagency Career Transition Assistance Plan (ICTAP) eligibles. * Defense Civilian Intelligence Personnel System (DCIPS) eligibles. * All Federal employees serving on a career or career-conditional appointment. * Department of Defense employees serving on a Career or Career Conditional Appointment. * Current Army employees with competitive status (includes Army employees serving on a career or career-conditional appointment). * Reinstatement eligibles. Qualifications: Click on link below to view qualification standard. General Schedule * 1. The Walter Reed Army Institute of Research is participating in an alternative personnel system known as the Personnel Demonstration Project. This position incorporates the GS-09 to GS-11 level. In keeping with the Demonstration pay fixing policies, employees already in the band will not receive a pay increase. 2. SPECIALIZED EXPERIENCE: Candidates for this position must show in their resume that they have one year of specialized experience and training that provided knowledge of histopathology procedures to ensure quality and promptness. This year of specialized experience must have included responsibility for performing unusual techniques, such as frozen microtomy, immunohistochemistry and plastic embedding / microtomy. * GS-06 and above: One year of experience directly related to this occupation equivalent to the next lower grade. Graduate education or an internship meets the experience required when it is directly related to the work of the position. * The experience described in your resume will be evaluated and screened for the Office of Personnel Management's basic qualifications requirements, and the skills needed to perform the duties of this position as described in this vacancy announcement. * Foreign education must be evaluated for U.S. equivalency in order to be considered for this position. Please include this information in your resume. * One year of experience in the same or similar work equivalent to at least the next lower grade or level requiring application of the knowledge, skills, and abilities of the position being filled. * Only degrees from an accredited college or university recognized by the Department of Education are acceptable to meet positive education requirements or to substitute education for experience. For additional information, please go to the Office of Personnel Management (OPM) and U.S. Department of Education websites at - http://www.opm.gov/qualifications and http://www.ed.gov/admins/finaid/accred/index.html Other Information: (Click on Other Information) * The Department of Defense (DoD) policy on employment of annuitants issued March 18, 2004 will be used in determining eligibility of annuitants. The DoD policy is available on http://www.cpms.osd.mil/fas/staffing/pdf/rem_ann.pdf * Permanent Change of Station (PCS) expenses are not authorized. * Temporary Duty (TDY) travel is 10 percent. Other Advantages: The Walter Reed Army Institute of Research is 10 minutes from Washington, D.C. If you would like more information about our installation, please visit our website at http://wrair-www.army.mil Other Requirements: (Click on Other Requirements) * Must be able to obtain and maintain a Secret security clearance. * A medical examination is required. * You will be required to provide proof of U.S. Citizenship. * Extended probationary period for the Engineers and Scientists Occupational Family will be three years. Extended probationary period for all other Occupational Families will be two years. * Male applicants born after December 31, 1959 must complete a Pre-Employment Certification Statement for Selective Service Registration. * Direct Deposit of Pay is Required. * This is a DOD Demonstration Project position. How to Apply: (Click on How to Apply) * Resumes must be received by the closing date of this announcement. * Self-nomination must be submitted by the closing date. * Resume must be on file in our centralized database. * Announcements close at 12:00am (midnight) Eastern Time. If your resume is currently in our central database, you may click here to Self Nominate Click here to use the Army Resume Builder to create your resume. Follow the instructions in this vacancy announcement to apply for the job. Point of Contact: Central Resume Processing Center, 410-306-0137, applicanthelp@cpsrxtp.belvoir.army.mil THE DEPARTMENT OF DEFENSE IS AN EQUAL OPPORTUNITY EMPLOYER ------------------------------ Message: 17 Date: Tue, 21 Mar 2006 06:52:25 -0800 (PST) From: beth zink Subject: [Histonet] hello To: histonet@pathology.swmed.edu Message-ID: <20060321145225.2646.qmail@web60123.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Could you please send me directions on how to ask questions and get responses? Thanks Beth --------------------------------- Yahoo! Mail Bring photos to life! New PhotoMail makes sharing a breeze. ------------------------------ Message: 18 Date: Tue, 21 Mar 2006 07:35:50 -0800 From: Patti Loykasek Subject: [Histonet] Job posting To: histonet Message-ID: Content-Type: text/plain; charset="ISO-8859-1" I'm posting this job opening for a friend in research who is having trouble with her internet connection. This position is located north of Seattle. HISTOLOGIST MDS Pharma Services, Division of Efficacy-Pharmacology, is a therapeutically focused contract research organization that specializes in bone and central nervous system (CNS) biologies. We are currently seeking an experienced histologist to primarily participate in the Company?s pre-clinical bone research programs. Responsibilities will include hard tissue processing, embedding, thin sectioning, ground sections (using the EXAKT system), staining of both thin and ground sections, and the development and validation of laboratory techniques in support of client-sponsored research programs. Specific experience in orthopedic histological techniques is highly desired. Qualified applicants will possess a BS/MS degree and/or HT/HTL certification, 4+ years of related histology experience and documented expertise in hard tissue preparation and sectioning (usually MMA), and use of the EXAKT system. Candidates with prior experience working in a GLP compliant laboratory and expertise with histomorphometry will be considered superior. MDS Pharma Services offers an energetic and challenging research environment with a competitive compensation and benefits package including equity participation. Interested applicants can apply via the MDS Pharma Services website located at www.mdsps.com . The position can be found by searching for jobs in the United States, and location, Bothell-West. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. ------------------------------ Message: 19 Date: Tue, 21 Mar 2006 09:38:51 -0600 From: "Charles.Embrey" Subject: RE: [Histonet] hello To: "beth zink" Cc: Histonet@pathology.swmed.edu Message-ID: Content-Type: text/plain; charset="us-ascii" You just did. That is all there is to it. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of beth zink Sent: Tuesday, March 21, 2006 8:52 AM To: histonet@pathology.swmed.edu Subject: [Histonet] hello Could you please send me directions on how to ask questions and get responses? Thanks Beth --------------------------------- Yahoo! Mail Bring photos to life! New PhotoMail makes sharing a breeze. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 20 Date: Tue, 21 Mar 2006 11:23:04 -0500 From: "Monfils, Paul" Subject: RE: [Histonet] oysters larvae again To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <09C945920A6B654199F7A58A1D7D1FDE017176A4@lsexch.lsmaster.lifespan.org> Content-Type: text/plain; charset="iso-8859-1" I don't have a background in ISH, so I can't offer an opinion on the best fixative for that purpose. Regarding your second question, while I'm not familiar with either fixative, I can see that the Davidson fixative, containing 10% acetic acid and no buffers, would decalcify (dissolve) larval oyster shells. But the MEMPFA-T appears to be made up in pH 7.4 buffer, in which case I don't see how it would decalcify. In any case, I am wondering why you are using a formaldehyde-based fixative for TEM? Ordinarily a glutaraldehyde-based fixative would be preferable for TEM. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > Anne-Sophie MARTINEZ > Sent: Tuesday, March 21, 2006 5:13 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] oysters larvae again > > Hello, > (1) I plan to fix oysters larvae/juveniles (4 month) for in toto ISH. > Which fixative is the best : Davidson (10% formaldehyde 37-40%, 30% > ethanol 95%, 60% seawater, 10% acetic acid) or MEMPFA-T (0,1 M Mops pH > 7,4; 2 mM EGTA; 1 mM MgSO4; 4% paraformaldehyde; 0,1% Tween 20)? > (2) Would it be possible to use the MEMPFA-T to fix and "decalcify" the > shelves of the juveniles for TEM (embedding in type lowyril, unicril, > LR-white resins) instead of classical fixative (4% PFA) and then EDTA? > Thank you very much again. > Anne-Sophie > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 21 Date: Tue, 21 Mar 2006 17:23:48 +0100 From: b003046@nf.au.dk Subject: [Histonet] Lineage Negative Cells To: "histonet@lists.utsouthwestern.edu" Message-ID: <1142958228.44202894e08a0@webmail.nf.au.dk> Content-Type: text/plain; charset=ISO-8859-1 Hi, I am looking for a method to exclude lineage negative cells (Lin-) in rats in immunohistochemistry (paraffin-embedded tissue). Which antibodies should I use and is it possible to buy a detection kit? Any help would be appreciated. kind regards Mette K. Hagensen ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 28, Issue 33 **************************************** Confidential: This electronic message and all contents contain information from BayCare Health System which may be privileged, confidential or otherwise protected from disclosure. The information is intended to be for the addressee only. If you are not the addressee, any disclosure, copy, distribution or use of the contents of this message is prohibited. If you have received this electronic message in error, please notify the sender and destroy the original message and all copies. From rjacquet <@t> hotmail.com Tue Mar 21 14:39:12 2006 From: rjacquet <@t> hotmail.com (Robin Jacquet) Date: Tue Mar 21 14:39:19 2006 Subject: [Histonet] Re RNA Later and cryosect In-Reply-To: Message-ID: Hi, We've found a quick rinse in cold DEPC treated water before embedding in OCT helps. But we do hard tissues and so I'm not sure about pancreas. Robin Jacquet From SHARON.OSBORN <@t> SPCORP.COM Tue Mar 21 13:20:35 2006 From: SHARON.OSBORN <@t> SPCORP.COM (Osborn, Sharon) Date: Tue Mar 21 14:56:12 2006 Subject: [Histonet] RE: mucicarmine Message-ID: <9A919A5D70313A4D9C56A025710874080C71C7@kenmsg40.us.schp.com> Dorothy, I did the mucicarmine for a year on cryptococcus research project. Therefore, I have a procedure that works great. Basically, it is the American Master Tech Scientific Muci-10 Procedure with modifications that we found made improvements to the staining procedure. If interested, I am happy to send the procedure to you. We kept the Muci-10 concentrate in the refrigerator and let it come to room Temp before use. Sharon Osborn DNAX, SP BioPharma Palo Alto, CA 650.496.6539 Message: 5 Date: Mon, 20 Mar 2006 09:28:54 -0600 From: "Webb, Dorothy L" Subject: [Histonet] mucicarmine To: Histonet@lists.utsouthwestern.edu Message-ID: <0E394B648E5284478A6CCB78E5AFDA270171FCE1@hpes1.HealthPartners.int> Content-Type: text/plain; charset="US-ASCII" We have been doing our mucicarmine on the Nexus special stainer and due to the fact that we do not do a lot of these, I decided to bring it back to handstaining. I am using the Southgate's modification of Mayer's Technique and having a very hard time getting the stain to look nice and sharp! Does anyone have any procedures they use or suggestions as to what could be our problem? Our muci is not staining the bright red and even the nuclei are not stained very dark. We have tried various times and strengths of the mucicarmine and even from two different companies! Help and thanks for any and all suggestions! _ ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From sbreeden <@t> nmda.nmsu.edu Tue Mar 21 15:24:24 2006 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Mar 21 15:24:29 2006 Subject: [Histonet] How come....? Message-ID: How come I get answers to my posting before I ever SEE the latest histonet? Hmmm....? Is it because New Mexico's internet-work is still operated by Pony Express? Or was it telegraph? Can't remember... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services POBox 4700 Albuquerque, NM 87196 (505)841-2576 (505)841-2518 FAX From POWELL_SA <@t> Mercer.edu Tue Mar 21 15:31:15 2006 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Tue Mar 21 15:31:46 2006 Subject: [Histonet] How come....? In-Reply-To: Message-ID: <01M0BNAY4Q168WZYEC@Macon2.Mercer.edu> The pigeons are tired. :) sp -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Tuesday, March 21, 2006 4:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How come....? How come I get answers to my posting before I ever SEE the latest histonet? Hmmm....? Is it because New Mexico's internet-work is still operated by Pony Express? Or was it telegraph? Can't remember... Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services POBox 4700 Albuquerque, NM 87196 (505)841-2576 (505)841-2518 FAX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sbreeden <@t> nmda.nmsu.edu Tue Mar 21 15:48:52 2006 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Mar 21 15:48:56 2006 Subject: [Histonet] Internet Speedless Message-ID: So, now I have the Friday Hour of Fuming subject all ready for this Friday. So bear with me, all this joviality. My work is done for the day and I'm just having a bit of R&R. Have a great rest-of-YOUR-day, folks! Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services POBox 4700 Albuquerque, NM 87196 (505)841-2576 (505)841-2518 FAX From jstn192 <@t> yahoo.com Tue Mar 21 16:42:40 2006 From: jstn192 <@t> yahoo.com (Justin Thomas) Date: Tue Mar 21 16:42:42 2006 Subject: [Histonet] RE: Inexpensive microtome knife sharpening Message-ID: <20060321224240.13786.qmail@web35712.mail.mud.yahoo.com> Hello all, this is Dr. Thomas again, of C.A. The same company that has inexpensive microwave accessories also has a microtome knife sharpening and reconditioning service. If you are looking to cut laboratory and hospital cost, this company is who you should turn to with the purchasing of microwave accessories and your knife sharpening and reconditioning. They resharpen/recondition all our knifes now, we are extremely satisfied with their prices and services. Give me your contact info if you want me to forward it the this company. -thanks, Dr. Thomas --------------------------------- Yahoo! Mail Bring photos to life! New PhotoMail makes sharing a breeze. From jstn192 <@t> yahoo.com Tue Mar 21 16:42:43 2006 From: jstn192 <@t> yahoo.com (Justin Thomas) Date: Tue Mar 21 16:42:47 2006 Subject: [Histonet] RE: Inexpensive microtome knife sharpening Message-ID: <20060321224243.73382.qmail@web35714.mail.mud.yahoo.com> Hello all, this is Dr. Thomas again, of C.A. The same company that has inexpensive microwave accessories also has a microtome knife sharpening and reconditioning service. If you are looking to cut laboratory and hospital cost, this company is who you should turn to with the purchasing of microwave accessories and your knife sharpening and reconditioning. They resharpen/recondition all our knifes now, we are extremely satisfied with their prices and services. Give me your contact info if you want me to forward it the this company. -thanks, Dr. Thomas --------------------------------- Yahoo! Mail Use Photomail to share photos without annoying attachments. From Rcartun <@t> harthosp.org Tue Mar 21 17:20:34 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Mar 21 17:21:16 2006 Subject: [Histonet] p16 antibody Message-ID: We have been using Novocastra's p16 antibody (distributed by Vision BioSystems here in the USA). I have been told that their antibody is back-ordered. I know DAKO sells a p16 kit, but I'm only interested in getting the antibody. Are there any other sources for p16 that work in formalin-fixed, paraffin-embedded tissues? Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From ylee <@t> bccancer.bc.ca Tue Mar 21 17:26:09 2006 From: ylee <@t> bccancer.bc.ca (Lee, Yin Ping) Date: Tue Mar 21 17:26:15 2006 Subject: [Histonet] rabbit monoclonal ER-SP1 and PR-SP2 Message-ID: <57AD92D1EDD1854BAAC02DD7DDAF0E360172E023@srvex10.phsabc.ehcnet.ca> We are testing rabbit monoclonal ER-SP1 and PR-SP2 from Vector. Can anyone share the experience with us, such as antigen retrevial method, dilution etc? Thanks in advance! Yin Ping Lee Histopathology BC Cancer Agency Vancouver, BC Canada From dpconsult <@t> earthlink.net Tue Mar 21 17:29:51 2006 From: dpconsult <@t> earthlink.net (HISTOLOGY SOCIETY OF OHIO) Date: Tue Mar 21 17:30:06 2006 Subject: [Histonet] Histology Society of Ohio - 32nd Annual Meeting - May 12 - 13, 2006 Message-ID: HISTOLOGY SOCIETY OF OHIO Please mark your calendars for May 12th and 13th. Our 32nd Annual Meeting will be held at the Fairborn Holiday Inn We will send details when they are finalized. Thank You. Mary Abbuhl President mary.abbuhl@uhhs.com From kwuny <@t> email.cs.nsw.gov.au Tue Mar 21 17:39:14 2006 From: kwuny <@t> email.cs.nsw.gov.au (Young Kwun) Date: Tue Mar 21 17:35:20 2006 Subject: [Histonet] p16 antibody In-Reply-To: Message-ID: <200603221035549.SM03936@crgcsls814> Richard, NeoMarkers (LAB VISION) has several p16 monoclonal antibodies for FFPE tissues. Biocare has one p16 clone (JC8) too. We've been using NeoMarkers p16 (clone 16P07) and happy with the results. Cheers, Young Young Kwun Senior Hospital Scientist Dept of Anatomical Pathology Concord Hospital Concord NSW 2139 Australia Phone:61-2-9767 6075 Fax:61-2-9767 8427 Email:kwuny@email.cs.nsw.gov.au -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Wednesday, 22 March 2006 10:21 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 antibody We have been using Novocastra's p16 antibody (distributed by Vision BioSystems here in the USA). I have been told that their antibody is back-ordered. I know DAKO sells a p16 kit, but I'm only interested in getting the antibody. Are there any other sources for p16 that work in formalin-fixed, paraffin-embedded tissues? Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____________________________________________________________________ This email has been scanned for the Sydney South West Area Health Service by the MessageLabs Email Security System. SSWAHS regularly monitors emails and attachments to ensure compliance with the NSW Government's Electronic Messaging Policy. "This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please destroy it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of the Sydney South West Area Health Service." From Linresearch <@t> aol.com Tue Mar 21 17:55:02 2006 From: Linresearch <@t> aol.com (Linresearch@aol.com) Date: Tue Mar 21 17:55:13 2006 Subject: [Histonet] Ultracut Microtome Message-ID: <27b.72072f3.3151ec56@aol.com> Hi, I am in need of an operator manual for the MT-7000, Ultracut EM microtome. I would apppreciate a copy or a source of one. Lin From RCHIOVETTI <@t> aol.com Tue Mar 21 21:23:12 2006 From: RCHIOVETTI <@t> aol.com (RCHIOVETTI@aol.com) Date: Tue Mar 21 21:23:25 2006 Subject: [Histonet] Ultracut Microtome Message-ID: <334.755bcf.31521d20@aol.com> In a message dated 3/21/2006 4:55:41 PM US Mountain Standard Time, Linresearch@aol.com writes: > Hi, > I am in need of an operator manual for the MT-7000, Ultracut EM microtome. > I would apppreciate a copy or a source of one. > Lin > Hi Lin, The MT-7000 Ultramicrotome was made by RMC, which is now part of Boeckeler Instruments in Tucson, Arizona. You can contact them at: 800.552.2262 Or on the Internet at: www.rmcproducts.com Best regards, Bob Robert (Bob) Chiovetti, Ph.D. The Microscope Works Arizona's Microscopy Resource 132 North Elster Drive Tucson, AZ 85710-3212 USA Tel./Fax 520-546-4986 Member, Arizona Small Business Association - ASBA From Jacqueline.Malam <@t> rli.mbht.nhs.uk Wed Mar 22 02:33:44 2006 From: Jacqueline.Malam <@t> rli.mbht.nhs.uk (Malam Jacqueline) Date: Wed Mar 22 02:34:05 2006 Subject: [Histonet] P16 source Message-ID: Try Vector labs - web site is www.vectorlabs.com Jacqui Malam Lancaster UK DISCLAIMER: This e-mail is confidential and privileged. If you are not the intended recipient please accept our apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform postmaster@rli.mbht.nhs.uk that this message has gone astray before deleting it. Comments or opinions expressed in this email are those of their respective contributors only. The views expressed do not represent the views of the Trust, its management or employees. Morecambe Bay Hospitals NHS Trust is not responsible and disclaims any and all liability for the content of comments written within.Thank you for your co-operation. From David.Muskett <@t> RLC.NHS.UK Wed Mar 22 04:46:43 2006 From: David.Muskett <@t> RLC.NHS.UK (Muskett David) Date: Wed Mar 22 04:46:52 2006 Subject: [Histonet] Meditech - Snomed codes Message-ID: Dear Histonetters I am writing with a query about Meditech and Snomed codes. I am currently struggling entering the full range of codes present in the chunky micro glossary book. Is any one else suffering with the same problem? We have contacted Meditech but they don't seem very sure what we are complaining about. If anyone has any SOPs they would be willing to share I would be very grateful. Regards David David Muskett Chief Biomedical Scientist, Histology Royal Liverpool Children's NHS Trust Eaton Road Liverpool L12 2AP Internal telephone x3615 Telephone (0151) 293 3656 Fax (0151) 293 3617 http://nhsald02/histopathology From jnocito <@t> satx.rr.com Wed Mar 22 06:08:42 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Mar 22 06:08:47 2006 Subject: [Histonet] Flame Cabinets Message-ID: <00fd01c64da9$5d2d00c0$e8bd0b43@yourxhtr8hvc4p> With the risk of being flamed, I'm having a large discussion (ok an argument) with my CEO and his sidekick about have flame cabinets vented. We are supposed to move into our new building in two days and I'm getting stonewalled by the engineer and the plumber about venting the flame cabinets. Of course, I've been stonewalled the entire process. The engineer is about 6'6", I'm 5'7" and I want to rip his eyes out and do something really nasty to his eye sockets, but that's another story. The sidekick came from our largest competitor because he was a friend. I know, we won't go there. The plans I set up are not the plans now. The first thing I wanted was to have the flame cabinets vented (that was back in last October). Now it's a problem. I think the dumb ass plumber doesn't want to do it. Yesterday, I had to go around all of San Antonio just to find plugs to plug the holes in the cabinets where they were vented in my current lab because the plumber didn't want to have the responsibility of the cabinets. Oh yeah, by the way, the name of the company is Bent Plumbing, no kidding. This should have alerted the powers at be. Thanks for you help. I just don't have the time to research this. Flame on. Joe the Toe From mparker <@t> epl-inc.com Wed Mar 22 06:44:33 2006 From: mparker <@t> epl-inc.com (Mary Parker) Date: Wed Mar 22 06:44:33 2006 Subject: [Histonet] unsubscribe Message-ID: Histology Lab Manager EPL, Inc. PO Box 12766 RTP, N.C. 27709 919 998-9407 From GDawson <@t> dynacaremilwaukee.com Wed Mar 22 07:19:18 2006 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Wed Mar 22 07:19:22 2006 Subject: [Histonet] p16 antibody Message-ID: Richard, I like Cell Marque's p16 antibody very much. Glen Dawson IHC Manager Milwaukee, WI -----Original Message----- From: Richard Cartun [mailto:Rcartun@harthosp.org] Sent: Tuesday, March 21, 2006 5:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] p16 antibody We have been using Novocastra's p16 antibody (distributed by Vision BioSystems here in the USA). I have been told that their antibody is back-ordered. I know DAKO sells a p16 kit, but I'm only interested in getting the antibody. Are there any other sources for p16 that work in formalin-fixed, paraffin-embedded tissues? Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike.kirby <@t> nhls.ac.za Wed Mar 22 07:57:03 2006 From: mike.kirby <@t> nhls.ac.za (Mike Kirby) Date: Wed Mar 22 07:52:53 2006 Subject: [Histonet] Training Med Techs - some candid comments Message-ID: <452D0F6B16AA6E4092F6D3E9A9D97A620F7D7C@nhlsgpm002.NHLS.AC.ZA> Dear Ms Orr. You may not have wanted a fight on your hands but now you've got one! Climbs on soapbox and starts tirade! Your comment " Training a MT to be a Histo Tech is like trying to train a policeman to be a fireman" rather sticks in the craw, and I would like to follow with a counter comment, "Can a Histo Tech be trained to be a fireman?" as anyone can be shown how to connect a hose to a hydrant and point it at the flames. Granted, Histopatholgy is very much a "hands on" profession, requiring fine manual dexterity and concentration, but it's no better than operating a high output Biochemistry/ Haematology analyser or cross-matching a pint of blood, where a wrong result can kill a patient. Plus you operate under a fraction of the stress we are subjected to - try working for a full day, and then doing another 13 hours of call out duty, and then you are expected to report for normal duty next day! It's a gross insult to insinuate that ours is a "job" while yours is a "career". As students, we spent 5 - 6 months working in every division that was available in the lab - Chempath, Haem, Parasitology, Micro, Cyto, Immunology, Human genetics, blood transfusion, and yes, even Histopath. When we passed our finals, we were allowed to choose which discipline we wanted to further our careers, and each year, without fail, the majority would choose anything but Histopath, as it was considered a boring "dead end division" (Yes, pun intended). As for management positions, if you can run a Chempath or Micro Dept, then you can run a Histopath Dept, as the same managerial systems apply, regardless of the discipline, it's just the practical applications of the work in hand that differ. Histopathology is just one of the services in the medical world, you don't walk on water, and neither do we. As far as I am concerned, once trained as a general Med Tech, you can be trained in virtually any other discipline, unless you are like "two left thumbs" me, who after 35 years on the bench, still cannot cut a half decent section or make a passable blood smear. (But I am good as a manager!) End of tirade - climbs off soapbox, puts on helmet, and climbs into bunker, to await the verbal barrage that's about to be unleashed................ Mr.M.Kirby Johannesburg South Africa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Orr, Rebecca Sent: 17 March 2006 17:04 To: histonet@lists.utsouthwestern.edu Cc: Delk, Linda Subject: [Histonet] Training Med Techs Hello everyone. I would appreciate any feedback from those of you who may have had to train MT's (ASCP) to work in Histology. They would be trained as histo techs with the intent to promote them into Anatomic Pathology (Histology) management positions. Candid comments welcome, especially from MT's who now work in histology! To me it would be like trying to train a policeman to be a fireman, it's a career, not a job, right? We see a HT shortage in the Chicago area, but I am unsure how to address this. Degreed individuals have proven critical thinking skills via a traditional education pathway, so I see the advantages, but to ignore very capable HT managers with proven management and organizational skills via non traditional pathways is becoming an issue with me. I mean it's not like Non degreed HT's are stooopid or something. Thank you Becky Becky Orr CLA,HT(ASCP) IHC Lead Evanston Northwestern Healthcare 847-570-2771 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************** The views expressed in this email are, unless otherwise stated, those of the author and not those of the National Health Laboratory Services or its management. The information in this e-mail is confidential and is intended solely for the addressee. Access to this e-mail by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken or omitted in reliance on this, is prohibited and may be unlawful. Whilst all reasonable steps are taken to ensure the accuracy and integrity of information and data transmitted electronically and to preserve the confidentiality thereof, no liability or responsibility whatsoever is accepted if information or data is, for whatever reason, corrupted or does not reach its intended destination. *********************************************************************************** From cpomajzl <@t> cpllabs.com Wed Mar 22 08:12:11 2006 From: cpomajzl <@t> cpllabs.com (Chris Pomajzl) Date: Wed Mar 22 08:09:49 2006 Subject: [Histonet] Training Med Techs - some candid comments References: <452D0F6B16AA6E4092F6D3E9A9D97A620F7D7C@nhlsgpm002.NHLS.AC.ZA> Message-ID: <004101c64dba$9d8765f0$26fca8c0@CSP> Lighten up Frances! ----- Original Message ----- From: "Mike Kirby" To: "Histonet (E-mail)" Sent: Wednesday, March 22, 2006 7:57 AM Subject: [Histonet] Training Med Techs - some candid comments Dear Ms Orr. You may not have wanted a fight on your hands but now you've got one! Climbs on soapbox and starts tirade! Your comment " Training a MT to be a Histo Tech is like trying to train a policeman to be a fireman" rather sticks in the craw, and I would like to follow with a counter comment, "Can a Histo Tech be trained to be a fireman?" as anyone can be shown how to connect a hose to a hydrant and point it at the flames. Granted, Histopatholgy is very much a "hands on" profession, requiring fine manual dexterity and concentration, but it's no better than operating a high output Biochemistry/ Haematology analyser or cross-matching a pint of blood, where a wrong result can kill a patient. Plus you operate under a fraction of the stress we are subjected to - try working for a full day, and then doing another 13 hours of call out duty, and then you are expected to report for normal duty next day! It's a gross insult to insinuate that ours is a "job" while yours is a "career". As students, we spent 5 - 6 months working in every division that was available in the lab - Chempath, Haem, Parasitology, Micro, Cyto, Immunology, Human genetics, blood transfusion, and yes, even Histopath. When we passed our finals, we were allowed to choose which discipline we wanted to further our careers, and each year, without fail, the majority would choose anything but Histopath, as it was considered a boring "dead end division" (Yes, pun intended). As for management positions, if you can run a Chempath or Micro Dept, then you can run a Histopath Dept, as the same managerial systems apply, regardless of the discipline, it's just the practical applications of the work in hand that differ. Histopathology is just one of the services in the medical world, you don't walk on water, and neither do we. As far as I am concerned, once trained as a general Med Tech, you can be trained in virtually any other discipline, unless you are like "two left thumbs" me, who after 35 years on the bench, still cannot cut a half decent section or make a passable blood smear. (But I am good as a manager!) End of tirade - climbs off soapbox, puts on helmet, and climbs into bunker, to await the verbal barrage that's about to be unleashed................ Mr.M.Kirby Johannesburg South Africa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Orr, Rebecca Sent: 17 March 2006 17:04 To: histonet@lists.utsouthwestern.edu Cc: Delk, Linda Subject: [Histonet] Training Med Techs Hello everyone. I would appreciate any feedback from those of you who may have had to train MT's (ASCP) to work in Histology. They would be trained as histo techs with the intent to promote them into Anatomic Pathology (Histology) management positions. Candid comments welcome, especially from MT's who now work in histology! To me it would be like trying to train a policeman to be a fireman, it's a career, not a job, right? We see a HT shortage in the Chicago area, but I am unsure how to address this. Degreed individuals have proven critical thinking skills via a traditional education pathway, so I see the advantages, but to ignore very capable HT managers with proven management and organizational skills via non traditional pathways is becoming an issue with me. I mean it's not like Non degreed HT's are stooopid or something. Thank you Becky Becky Orr CLA,HT(ASCP) IHC Lead Evanston Northwestern Healthcare 847-570-2771 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************************************************** ****** The views expressed in this email are, unless otherwise stated, those of the author and not those of the National Health Laboratory Services or its management. The information in this e-mail is confidential and is intended solely for the addressee. Access to this e-mail by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken or omitted in reliance on this, is prohibited and may be unlawful. Whilst all reasonable steps are taken to ensure the accuracy and integrity of information and data transmitted electronically and to preserve the confidentiality thereof, no liability or responsibility whatsoever is accepted if information or data is, for whatever reason, corrupted or does not reach its intended destination. **************************************************************************** ******* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Wed Mar 22 09:01:03 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Mar 22 09:01:13 2006 Subject: [Histonet] Training Med Techs - some candid comments Message-ID: <83AACDB0810528418AA106F9AE9B7F7E01D34E26@sjhaexc02.sjha.org> One of the problems with email is not being able to see how the post is being presented. Mike, in the US we Histotechs have to travel the road of the red-headed step child, and fight for recognition that techs outside the US have without question. At least you are all lumped into the same pile, as well as I can see - all well educated and trained in all the lab sciences. In the US, Histology is now left out of Med Tech training programs. Anatomic Pathology is very foreign to most Med techs - to give you an example... Several years ago when setting up the computer programs for AP - with a Med Tech directing - we in AP including the docs, were all trying to make panels for all the stains, and trying to explain that we didn't do panels on everything. We finally realized it was computer language and made it though. The obvious difference came with the Med Tech questioned why the patient had to come in every hour for a cervical biopsy when I made specimen codes for Cervical Biopsy - 1:00 and so forth around the clock. I don't think Becky meant anything detrimental - just musing about the differences, and how just because we are red headed step children we can't be look at as managers... My 2 cents. J Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 404-851-7831 - fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Chris Pomajzl Sent: Wednesday, March 22, 2006 9:12 AM To: HISTONET Subject: Re: [Histonet] Training Med Techs - some candid comments Lighten up Frances! ----- Original Message ----- From: "Mike Kirby" To: "Histonet (E-mail)" Sent: Wednesday, March 22, 2006 7:57 AM Subject: [Histonet] Training Med Techs - some candid comments Dear Ms Orr. You may not have wanted a fight on your hands but now you've got one! Climbs on soapbox and starts tirade! Your comment " Training a MT to be a Histo Tech is like trying to train a policeman to be a fireman" rather sticks in the craw, and I would like to follow with a counter comment, "Can a Histo Tech be trained to be a fireman?" as anyone can be shown how to connect a hose to a hydrant and point it at the flames. Granted, Histopatholgy is very much a "hands on" profession, requiring fine manual dexterity and concentration, but it's no better than operating a high output Biochemistry/ Haematology analyser or cross-matching a pint of blood, where a wrong result can kill a patient. Plus you operate under a fraction of the stress we are subjected to - try working for a full day, and then doing another 13 hours of call out duty, and then you are expected to report for normal duty next day! It's a gross insult to insinuate that ours is a "job" while yours is a "career". As students, we spent 5 - 6 months working in every division that was available in the lab - Chempath, Haem, Parasitology, Micro, Cyto, Immunology, Human genetics, blood transfusion, and yes, even Histopath. When we passed our finals, we were allowed to choose which discipline we wanted to further our careers, and each year, without fail, the majority would choose anything but Histopath, as it was considered a boring "dead end division" (Yes, pun intended). As for management positions, if you can run a Chempath or Micro Dept, then you can run a Histopath Dept, as the same managerial systems apply, regardless of the discipline, it's just the practical applications of the work in hand that differ. Histopathology is just one of the services in the medical world, you don't walk on water, and neither do we. As far as I am concerned, once trained as a general Med Tech, you can be trained in virtually any other discipline, unless you are like "two left thumbs" me, who after 35 years on the bench, still cannot cut a half decent section or make a passable blood smear. (But I am good as a manager!) End of tirade - climbs off soapbox, puts on helmet, and climbs into bunker, to await the verbal barrage that's about to be unleashed................ Mr.M.Kirby Johannesburg South Africa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Orr, Rebecca Sent: 17 March 2006 17:04 To: histonet@lists.utsouthwestern.edu Cc: Delk, Linda Subject: [Histonet] Training Med Techs Hello everyone. I would appreciate any feedback from those of you who may have had to train MT's (ASCP) to work in Histology. They would be trained as histo techs with the intent to promote them into Anatomic Pathology (Histology) management positions. Candid comments welcome, especially from MT's who now work in histology! To me it would be like trying to train a policeman to be a fireman, it's a career, not a job, right? We see a HT shortage in the Chicago area, but I am unsure how to address this. Degreed individuals have proven critical thinking skills via a traditional education pathway, so I see the advantages, but to ignore very capable HT managers with proven management and organizational skills via non traditional pathways is becoming an issue with me. I mean it's not like Non degreed HT's are stooopid or something. Thank you Becky Becky Orr CLA,HT(ASCP) IHC Lead Evanston Northwestern Healthcare 847-570-2771 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************************************************** ****** The views expressed in this email are, unless otherwise stated, those of the author and not those of the National Health Laboratory Services or its management. The information in this e-mail is confidential and is intended solely for the addressee. Access to this e-mail by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken or omitted in reliance on this, is prohibited and may be unlawful. Whilst all reasonable steps are taken to ensure the accuracy and integrity of information and data transmitted electronically and to preserve the confidentiality thereof, no liability or responsibility whatsoever is accepted if information or data is, for whatever reason, corrupted or does not reach its intended destination. **************************************************************************** ******* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From jstn192 <@t> yahoo.com Wed Mar 22 09:05:48 2006 From: jstn192 <@t> yahoo.com (Justin Thomas) Date: Wed Mar 22 09:05:51 2006 Subject: [Histonet] ATTN: Tissue Processors (microwave users) Message-ID: <20060322150548.75930.qmail@web35711.mail.mud.yahoo.com> I am head of histology/pathology at a hospital in California, and I am putting the word out for members to respond....there is a company that can make identical microwave/any other accessories made of hard or soft material to accommodate lab equipment relatively economical! I have ordered a number of different accessories from this company, and they have great service and pricing. I think each member will be pleased with their services. Just reply to my email if you are interested with your contact infomation and i will forward it to the company. -thanks, Thomas --------------------------------- Yahoo! Mail Bring photos to life! New PhotoMail makes sharing a breeze. From Charles.Embrey <@t> carle.com Wed Mar 22 09:30:38 2006 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Wed Mar 22 09:30:52 2006 Subject: [Histonet] Training Med Techs - some candid comments Message-ID: Wow Mike, I think you misunderstood Becky's comments. I don't think she ever meant that Histology was a career and Lab was a job. She meant that both are career choices not just jobs. The big problem with e-mail is that it is so limited in communicating precise thoughts that it is so easy to misunderstand someone's meaning. I guess that is why people started using smileys or "emoticons" ;) . You are partially right when you say that histology can be a "dead-end" job. With registration requirements in the US having been lower for HT vs. MT it was natural to assume that the better educated would make better supervisors. I have to agree that in a strict "paperwork only" management environment this makes sense but most histology supervisors need to be working, hands on people that can troubleshoot well. The HTL certification denotes the higher education and many of these individuals are used in management positions but there are simply not enough to go around. The newest trend, that I haven't seen mentioned in this thread, is the use of certified Pathologists' Assistants as managers. These individuals, by rule, possess at least a Bachelor's degree with the majority possessing a Master's degree. They normally do a pretty good job supervising but most are not very good at troubleshooting unless they had a prior job as an HT. Always remember that level of degree is not always directly proportional to ability. Some of the more intelligent, wisest, people I know are high school diploma histotechs. I guess the bottom line is that virtually anyone can be trained to adequately manage from the paperwork standpoint and prior knowledge of the task makes one more effective as a "hands on" manager. Managing people and motivating them however, takes real skill and talent. A talented, well trained HT can make a wonderful manager. Becky is simply experiencing the same shortage of histology personnel the rest of the country is facing and asking a simple question to try and decide what is best for her lab. You bit her head off unnecessarily and owe her an apology. Charles Embrey PA(ASCP), HT Histology Manager Urbana IL -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Kirby Sent: Wednesday, March 22, 2006 7:57 AM To: Histonet (E-mail) Subject: [Histonet] Training Med Techs - some candid comments Dear Ms Orr. You may not have wanted a fight on your hands but now you've got one! Climbs on soapbox and starts tirade! Your comment " Training a MT to be a Histo Tech is like trying to train a policeman to be a fireman" rather sticks in the craw, and I would like to follow with a counter comment, "Can a Histo Tech be trained to be a fireman?" as anyone can be shown how to connect a hose to a hydrant and point it at the flames. Granted, Histopatholgy is very much a "hands on" profession, requiring fine manual dexterity and concentration, but it's no better than operating a high output Biochemistry/ Haematology analyser or cross-matching a pint of blood, where a wrong result can kill a patient. Plus you operate under a fraction of the stress we are subjected to - try working for a full day, and then doing another 13 hours of call out duty, and then you are expected to report for normal duty next day! It's a gross insult to insinuate that ours is a "job" while yours is a "career". As students, we spent 5 - 6 months working in every division that was available in the lab - Chempath, Haem, Parasitology, Micro, Cyto, Immunology, Human genetics, blood transfusion, and yes, even Histopath. When we passed our finals, we were allowed to choose which discipline we wanted to further our careers, and each year, without fail, the majority would choose anything but Histopath, as it was considered a boring "dead end division" (Yes, pun intended). As for management positions, if you can run a Chempath or Micro Dept, then you can run a Histopath Dept, as the same managerial systems apply, regardless of the discipline, it's just the practical applications of the work in hand that differ. Histopathology is just one of the services in the medical world, you don't walk on water, and neither do we. As far as I am concerned, once trained as a general Med Tech, you can be trained in virtually any other discipline, unless you are like "two left thumbs" me, who after 35 years on the bench, still cannot cut a half decent section or make a passable blood smear. (But I am good as a manager!) End of tirade - climbs off soapbox, puts on helmet, and climbs into bunker, to await the verbal barrage that's about to be unleashed................ Mr.M.Kirby Johannesburg South Africa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Orr, Rebecca Sent: 17 March 2006 17:04 To: histonet@lists.utsouthwestern.edu Cc: Delk, Linda Subject: [Histonet] Training Med Techs Hello everyone. I would appreciate any feedback from those of you who may have had to train MT's (ASCP) to work in Histology. They would be trained as histo techs with the intent to promote them into Anatomic Pathology (Histology) management positions. Candid comments welcome, especially from MT's who now work in histology! To me it would be like trying to train a policeman to be a fireman, it's a career, not a job, right? We see a HT shortage in the Chicago area, but I am unsure how to address this. Degreed individuals have proven critical thinking skills via a traditional education pathway, so I see the advantages, but to ignore very capable HT managers with proven management and organizational skills via non traditional pathways is becoming an issue with me. I mean it's not like Non degreed HT's are stooopid or something. Thank you Becky Becky Orr CLA,HT(ASCP) IHC Lead Evanston Northwestern Healthcare 847-570-2771 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************ ********** The views expressed in this email are, unless otherwise stated, those of the author and not those of the National Health Laboratory Services or its management. The information in this e-mail is confidential and is intended solely for the addressee. Access to this e-mail by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken or omitted in reliance on this, is prohibited and may be unlawful. Whilst all reasonable steps are taken to ensure the accuracy and integrity of information and data transmitted electronically and to preserve the confidentiality thereof, no liability or responsibility whatsoever is accepted if information or data is, for whatever reason, corrupted or does not reach its intended destination. ************************************************************************ *********** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DDDeltour <@t> mar.med.navy.mil Wed Mar 22 09:32:28 2006 From: DDDeltour <@t> mar.med.navy.mil (DDDeltour@mar.med.navy.mil) Date: Wed Mar 22 09:32:56 2006 Subject: [Histonet] Training Med Techs - some candid comments Message-ID: <3F500F8B416C554EBB21FF16642F72E959CE02@marxchg03.mar.med.navy.mil> Relax Mike. I think you missed the point completely. Becky did not disrespect the MT community. She is frustrated with the lack of good Histology techs in her area. Before histology schools, the only way to get into histology was to be a lab worker (med tech) and stumble into it. That practice is not recommended/preferred anymore. It is not because med techs are not capable to learn, but because histology has advanced where specialized training is needed for the individual. To Becky, I would say "build it and they will come". First realize that the shortage is not just in your area. You have to go above and beyond to recruit people to your area/facility. Try to work with your HR department (I know good luck) and compare the benefits, wages, and bonuses to other facilities in your area. After you have tried that compare your facility to the rest of the US. It may help if you can toss in a space heater too. Chicago is way cold. :) Douglas D. Deltour HT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Kirby Sent: Wednesday, March 22, 2006 8:57 AM To: Histonet (E-mail) Subject: [Histonet] Training Med Techs - some candid comments Dear Ms Orr. You may not have wanted a fight on your hands but now you've got one! Climbs on soapbox and starts tirade! Your comment " Training a MT to be a Histo Tech is like trying to train a policeman to be a fireman" rather sticks in the craw, and I would like to follow with a counter comment, "Can a Histo Tech be trained to be a fireman?" as anyone can be shown how to connect a hose to a hydrant and point it at the flames. Granted, Histopatholgy is very much a "hands on" profession, requiring fine manual dexterity and concentration, but it's no better than operating a high output Biochemistry/ Haematology analyser or cross-matching a pint of blood, where a wrong result can kill a patient. Plus you operate under a fraction of the stress we are subjected to - try working for a full day, and then doing another 13 hours of call out duty, and then you are expected to report for normal duty next day! It's a gross insult to insinuate that ours is a "job" while yours is a "career". As students, we spent 5 - 6 months working in every division that was available in the lab - Chempath, Haem, Parasitology, Micro, Cyto, Immunology, Human genetics, blood transfusion, and yes, even Histopath. When we passed our finals, we were allowed to choose which discipline we wanted to further our careers, and each year, without fail, the majority would choose anything but Histopath, as it was considered a boring "dead end division" (Yes, pun intended). As for management positions, if you can run a Chempath or Micro Dept, then you can run a Histopath Dept, as the same managerial systems apply, regardless of the discipline, it's just the practical applications of the work in hand that differ. Histopathology is just one of the services in the medical world, you don't walk on water, and neither do we. As far as I am concerned, once trained as a general Med Tech, you can be trained in virtually any other discipline, unless you are like "two left thumbs" me, who after 35 years on the bench, still cannot cut a half decent section or make a passable blood smear. (But I am good as a manager!) End of tirade - climbs off soapbox, puts on helmet, and climbs into bunker, to await the verbal barrage that's about to be unleashed................ Mr.M.Kirby Johannesburg South Africa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Orr, Rebecca Sent: 17 March 2006 17:04 To: histonet@lists.utsouthwestern.edu Cc: Delk, Linda Subject: [Histonet] Training Med Techs Hello everyone. I would appreciate any feedback from those of you who may have had to train MT's (ASCP) to work in Histology. They would be trained as histo techs with the intent to promote them into Anatomic Pathology (Histology) management positions. Candid comments welcome, especially from MT's who now work in histology! To me it would be like trying to train a policeman to be a fireman, it's a career, not a job, right? We see a HT shortage in the Chicago area, but I am unsure how to address this. Degreed individuals have proven critical thinking skills via a traditional education pathway, so I see the advantages, but to ignore very capable HT managers with proven management and organizational skills via non traditional pathways is becoming an issue with me. I mean it's not like Non degreed HT's are stooopid or something. Thank you Becky Becky Orr CLA,HT(ASCP) IHC Lead Evanston Northwestern Healthcare 847-570-2771 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************************************************** ****** The views expressed in this email are, unless otherwise stated, those of the author and not those of the National Health Laboratory Services or its management. The information in this e-mail is confidential and is intended solely for the addressee. Access to this e-mail by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken or omitted in reliance on this, is prohibited and may be unlawful. Whilst all reasonable steps are taken to ensure the accuracy and integrity of information and data transmitted electronically and to preserve the confidentiality thereof, no liability or responsibility whatsoever is accepted if information or data is, for whatever reason, corrupted or does not reach its intended destination. **************************************************************************** ******* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo.rogerson <@t> waht.swest.nhs.uk Wed Mar 22 09:51:41 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Mar 22 09:51:00 2006 Subject: [Histonet] Training Med Techs - some candid comments Message-ID: The analogies in the UK are MLAs who had no career structure but it has become apparent to me that they are a rich vein of BMSs (HistoTech). They are intelligent and committed but for a variety of reasons never got a degree. We now recognise them as Healthcare Scientist (which we all are in the UK Labs, Physiotherapy, etc.,) and they can be trained to the post of Healthcare Practitioner. They can also take a Foundation Degree to become a qualified BMS and prove to be committed Staff who rarely leaves. I think what needs to be done is to eradicate the barriers between your Staff groups and through training and education make it possible for one group to move to the next; the so called 'skills escalator'. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Weems, Joyce [mailto:JWEEMS@sjha.org] Sent: Wednesday, March 22, 2006 3:01 PM To: HISTONET Subject: RE: [Histonet] Training Med Techs - some candid comments One of the problems with email is not being able to see how the post is being presented. Mike, in the US we Histotechs have to travel the road of the red-headed step child, and fight for recognition that techs outside the US have without question. At least you are all lumped into the same pile, as well as I can see - all well educated and trained in all the lab sciences. In the US, Histology is now left out of Med Tech training programs. Anatomic Pathology is very foreign to most Med techs - to give you an example... Several years ago when setting up the computer programs for AP - with a Med Tech directing - we in AP including the docs, were all trying to make panels for all the stains, and trying to explain that we didn't do panels on everything. We finally realized it was computer language and made it though. The obvious difference came with the Med Tech questioned why the patient had to come in every hour for a cervical biopsy when I made specimen codes for Cervical Biopsy - 1:00 and so forth around the clock. I don't think Becky meant anything detrimental - just musing about the differences, and how just because we are red headed step children we can't be look at as managers... My 2 cents. J Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 404-851-7831 - fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Chris Pomajzl Sent: Wednesday, March 22, 2006 9:12 AM To: HISTONET Subject: Re: [Histonet] Training Med Techs - some candid comments Lighten up Frances! ----- Original Message ----- From: "Mike Kirby" To: "Histonet (E-mail)" Sent: Wednesday, March 22, 2006 7:57 AM Subject: [Histonet] Training Med Techs - some candid comments Dear Ms Orr. You may not have wanted a fight on your hands but now you've got one! Climbs on soapbox and starts tirade! Your comment " Training a MT to be a Histo Tech is like trying to train a policeman to be a fireman" rather sticks in the craw, and I would like to follow with a counter comment, "Can a Histo Tech be trained to be a fireman?" as anyone can be shown how to connect a hose to a hydrant and point it at the flames. Granted, Histopatholgy is very much a "hands on" profession, requiring fine manual dexterity and concentration, but it's no better than operating a high output Biochemistry/ Haematology analyser or cross-matching a pint of blood, where a wrong result can kill a patient. Plus you operate under a fraction of the stress we are subjected to - try working for a full day, and then doing another 13 hours of call out duty, and then you are expected to report for normal duty next day! It's a gross insult to insinuate that ours is a "job" while yours is a "career". As students, we spent 5 - 6 months working in every division that was available in the lab - Chempath, Haem, Parasitology, Micro, Cyto, Immunology, Human genetics, blood transfusion, and yes, even Histopath. When we passed our finals, we were allowed to choose which discipline we wanted to further our careers, and each year, without fail, the majority would choose anything but Histopath, as it was considered a boring "dead end division" (Yes, pun intended). As for management positions, if you can run a Chempath or Micro Dept, then you can run a Histopath Dept, as the same managerial systems apply, regardless of the discipline, it's just the practical applications of the work in hand that differ. Histopathology is just one of the services in the medical world, you don't walk on water, and neither do we. As far as I am concerned, once trained as a general Med Tech, you can be trained in virtually any other discipline, unless you are like "two left thumbs" me, who after 35 years on the bench, still cannot cut a half decent section or make a passable blood smear. (But I am good as a manager!) End of tirade - climbs off soapbox, puts on helmet, and climbs into bunker, to await the verbal barrage that's about to be unleashed................ Mr.M.Kirby Johannesburg South Africa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Orr, Rebecca Sent: 17 March 2006 17:04 To: histonet@lists.utsouthwestern.edu Cc: Delk, Linda Subject: [Histonet] Training Med Techs Hello everyone. I would appreciate any feedback from those of you who may have had to train MT's (ASCP) to work in Histology. They would be trained as histo techs with the intent to promote them into Anatomic Pathology (Histology) management positions. Candid comments welcome, especially from MT's who now work in histology! To me it would be like trying to train a policeman to be a fireman, it's a career, not a job, right? We see a HT shortage in the Chicago area, but I am unsure how to address this. Degreed individuals have proven critical thinking skills via a traditional education pathway, so I see the advantages, but to ignore very capable HT managers with proven management and organizational skills via non traditional pathways is becoming an issue with me. I mean it's not like Non degreed HT's are stooopid or something. Thank you Becky Becky Orr CLA,HT(ASCP) IHC Lead Evanston Northwestern Healthcare 847-570-2771 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************************************************** ****** The views expressed in this email are, unless otherwise stated, those of the author and not those of the National Health Laboratory Services or its management. The information in this e-mail is confidential and is intended solely for the addressee. Access to this e-mail by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken or omitted in reliance on this, is prohibited and may be unlawful. Whilst all reasonable steps are taken to ensure the accuracy and integrity of information and data transmitted electronically and to preserve the confidentiality thereof, no liability or responsibility whatsoever is accepted if information or data is, for whatever reason, corrupted or does not reach its intended destination. **************************************************************************** ******* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From GDawson <@t> dynacaremilwaukee.com Wed Mar 22 10:43:12 2006 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Wed Mar 22 10:43:14 2006 Subject: [Histonet] Training Med Techs - some candid comments Message-ID: Mr. Kirby, There is so much I'd like to say but my fellow histotechs have said most of it already. I must say that I absolutely LOVED the "we work under a fraction of the pressure as an MT" who must fret for many minutes before hitting the button on the machine that does all the work. Come on now, I'm getting sick to my stomach. I'm sure my fellow techs will be overly nice to a person who is completely undeserving so I won't grant you the same courtesy. Histology doesn't abide fools so do the world a favor and stay out of the field. Your disdain for histology and the people who do the work should steer you in the direction of getting off the histonet so it doesn't somehow "taint" you. Now, show some intelligence and don't act surprised when you get flamed by histotechs after you insult them on their list server. Glen Dawson IHC Manager Milwaukee, WI -----Original Message----- From: Chris Pomajzl [mailto:cpomajzl@cpllabs.com] Sent: Wednesday, March 22, 2006 8:12 AM To: HISTONET Subject: Re: [Histonet] Training Med Techs - some candid comments Lighten up Frances! ----- Original Message ----- From: "Mike Kirby" To: "Histonet (E-mail)" Sent: Wednesday, March 22, 2006 7:57 AM Subject: [Histonet] Training Med Techs - some candid comments Dear Ms Orr. You may not have wanted a fight on your hands but now you've got one! Climbs on soapbox and starts tirade! Your comment " Training a MT to be a Histo Tech is like trying to train a policeman to be a fireman" rather sticks in the craw, and I would like to follow with a counter comment, "Can a Histo Tech be trained to be a fireman?" as anyone can be shown how to connect a hose to a hydrant and point it at the flames. Granted, Histopatholgy is very much a "hands on" profession, requiring fine manual dexterity and concentration, but it's no better than operating a high output Biochemistry/ Haematology analyser or cross-matching a pint of blood, where a wrong result can kill a patient. Plus you operate under a fraction of the stress we are subjected to - try working for a full day, and then doing another 13 hours of call out duty, and then you are expected to report for normal duty next day! It's a gross insult to insinuate that ours is a "job" while yours is a "career". As students, we spent 5 - 6 months working in every division that was available in the lab - Chempath, Haem, Parasitology, Micro, Cyto, Immunology, Human genetics, blood transfusion, and yes, even Histopath. When we passed our finals, we were allowed to choose which discipline we wanted to further our careers, and each year, without fail, the majority would choose anything but Histopath, as it was considered a boring "dead end division" (Yes, pun intended). As for management positions, if you can run a Chempath or Micro Dept, then you can run a Histopath Dept, as the same managerial systems apply, regardless of the discipline, it's just the practical applications of the work in hand that differ. Histopathology is just one of the services in the medical world, you don't walk on water, and neither do we. As far as I am concerned, once trained as a general Med Tech, you can be trained in virtually any other discipline, unless you are like "two left thumbs" me, who after 35 years on the bench, still cannot cut a half decent section or make a passable blood smear. (But I am good as a manager!) End of tirade - climbs off soapbox, puts on helmet, and climbs into bunker, to await the verbal barrage that's about to be unleashed................ Mr.M.Kirby Johannesburg South Africa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Orr, Rebecca Sent: 17 March 2006 17:04 To: histonet@lists.utsouthwestern.edu Cc: Delk, Linda Subject: [Histonet] Training Med Techs Hello everyone. I would appreciate any feedback from those of you who may have had to train MT's (ASCP) to work in Histology. They would be trained as histo techs with the intent to promote them into Anatomic Pathology (Histology) management positions. Candid comments welcome, especially from MT's who now work in histology! To me it would be like trying to train a policeman to be a fireman, it's a career, not a job, right? We see a HT shortage in the Chicago area, but I am unsure how to address this. Degreed individuals have proven critical thinking skills via a traditional education pathway, so I see the advantages, but to ignore very capable HT managers with proven management and organizational skills via non traditional pathways is becoming an issue with me. I mean it's not like Non degreed HT's are stooopid or something. Thank you Becky Becky Orr CLA,HT(ASCP) IHC Lead Evanston Northwestern Healthcare 847-570-2771 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************************************************** ****** The views expressed in this email are, unless otherwise stated, those of the author and not those of the National Health Laboratory Services or its management. The information in this e-mail is confidential and is intended solely for the addressee. Access to this e-mail by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken or omitted in reliance on this, is prohibited and may be unlawful. Whilst all reasonable steps are taken to ensure the accuracy and integrity of information and data transmitted electronically and to preserve the confidentiality thereof, no liability or responsibility whatsoever is accepted if information or data is, for whatever reason, corrupted or does not reach its intended destination. **************************************************************************** ******* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cdemarinis <@t> SARATOGACARE.ORG Wed Mar 22 10:34:14 2006 From: cdemarinis <@t> SARATOGACARE.ORG (Demarinis, Carolyn) Date: Wed Mar 22 11:29:14 2006 Subject: [Histonet] CAP - ANP.11713 Phase II Message-ID: There is a new question on the CAP checklist: ANP.11713 - Phase II "Is there documented evidence of daily review of technical quality of histologic preparations by the pathologist?" Our present procedure is the supervisor/technologist documents daily review, but it is very clear that CAP is requiring the pathologist to initial a document daily proving they have reviewed the technical quality of slides. How are other hospitals satisfying this requirement? It seems crazy to make a pathologist initial a document daily, when it is evident they review hundreds of slides a day, and they would certainly report a problem to the supervisor when the stain is unacceptable. From RBARNHART <@t> summithealth.org Wed Mar 22 11:44:08 2006 From: RBARNHART <@t> summithealth.org (Rebecca Barnhart) Date: Wed Mar 22 11:44:48 2006 Subject: [Histonet] CAP - ANP.11713 Phase II Message-ID: I created a database that we enter our daily count (the date, first and last case number, the number of blocks and slides). Also there are 2 prompts to be checked by the pathologist, one for H & E and the other for cytology stain. We run a control as the first and last slide everyday. If the stain is ok he puts a check in both boxes, if there is a problem there is a spot to enter comments. I started doing it this way so that if there is a problem with the stain I can easily go back and see what cases it affected. I have a report that I can print to show the quality of stain if an inspector request it. Previously we had a sheet the pathologist kept at his desk and check each day if the stain was ok and make comments if not. I also use the spot for comments to document if we have changed something (trying a different stain, new paraffin, added time to a step on the processor or stainer, etc). So if there are questions or we need to compare slides (because of a new product or new procedure) I have a report that I run and I can easily pull the slides. I also use this database to enter all statistical information. So at any time I can give the pathologist or manager any number for anatomic pathology. >>> "Demarinis, Carolyn" 3/22/2006 11:34:14 AM >>> There is a new question on the CAP checklist: ANP.11713 - Phase II "Is there documented evidence of daily review of technical quality of histologic preparations by the pathologist?" Our present procedure is the supervisor/technologist documents daily review, but it is very clear that CAP is requiring the pathologist to initial a document daily proving they have reviewed the technical quality of slides. How are other hospitals satisfying this requirement? It seems crazy to make a pathologist initial a document daily, when it is evident they review hundreds of slides a day, and they would certainly report a problem to the supervisor when the stain is unacceptable. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Wed Mar 22 11:37:15 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Mar 22 12:29:41 2006 Subject: [Histonet] CAP - ANP.11713 Phase II Message-ID: <83AACDB0810528418AA106F9AE9B7F7E01D34E34@sjhaexc02.sjha.org> We give the control to the pathologist daily - who initials satisfactory or not... problems are dealt with by techs usually before path gets here tho. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Demarinis, Carolyn Sent: Wednesday, March 22, 2006 11:34 AM To: HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] CAP - ANP.11713 Phase II There is a new question on the CAP checklist: ANP.11713 - Phase II "Is there documented evidence of daily review of technical quality of histologic preparations by the pathologist?" Our present procedure is the supervisor/technologist documents daily review, but it is very clear that CAP is requiring the pathologist to initial a document daily proving they have reviewed the technical quality of slides. How are other hospitals satisfying this requirement? It seems crazy to make a pathologist initial a document daily, when it is evident they review hundreds of slides a day, and they would certainly report a problem to the supervisor when the stain is unacceptable. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From GoodwinD <@t> pahosp.com Wed Mar 22 11:46:10 2006 From: GoodwinD <@t> pahosp.com (Goodwin, Diana) Date: Wed Mar 22 12:29:46 2006 Subject: [Histonet] guidelines for Her2 i nterpretation Message-ID: <80CDD9C3FEEAFD4982B114C4A6DFD00EE4A3D4@uphsmbx2.UPHS.PENNHEALTH.PRV> I have been out of the loop with Her- 2 for a while, and the last I knew, it was left up to the individual labs to establish standards among the interpreting physicians for interpretation, pretty much based on the original Herceptest guidelines. Have any other standards been established? If so, would someone please provide me with a reference? Much obliged, Diana Goodwin Supervisor, Anatomic Pathology Pennsylvania Hospital Preston 655-C ph. 215-829-6532 fax 215-829-7564 e-mail goodwind@[pahosp.com From HornHV <@t> archildrens.org Wed Mar 22 11:54:56 2006 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Mar 22 12:29:50 2006 Subject: [Histonet] CAP - ANP.11713 Phase II Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C63038BDFFC@EMAIL.archildrens.org> We send out a QC sheet daily with slides for the day. The docs sign off on this sheet noting the quality of the slides and return it to our lab. On the sheet it is just a matter of them checking off the slide review and if they are acceptable or not. HH -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Demarinis, Carolyn Sent: Wednesday, March 22, 2006 10:34 AM To: HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] CAP - ANP.11713 Phase II There is a new question on the CAP checklist: ANP.11713 - Phase II "Is there documented evidence of daily review of technical quality of histologic preparations by the pathologist?" Our present procedure is the supervisor/technologist documents daily review, but it is very clear that CAP is requiring the pathologist to initial a document daily proving they have reviewed the technical quality of slides. How are other hospitals satisfying this requirement? It seems crazy to make a pathologist initial a document daily, when it is evident they review hundreds of slides a day, and they would certainly report a problem to the supervisor when the stain is unacceptable. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From ROrr <@t> enh.org Wed Mar 22 12:33:00 2006 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Wed Mar 22 12:33:05 2006 Subject: [Histonet] The MT thing Message-ID: AW I just love you guys! Everyone has good points and I appreciate Mike's input. The thing is I still don't have a supervisor and I'll be very sad to leave my cushy IHC queendom to go do it myself. Let's be done with this and move on. Becky Orr CLA,HT(ASCP) IHC Lead Evanston Northwestern Healthcare 847-570-2771 From meint002 <@t> umn.edu Wed Mar 22 13:19:26 2006 From: meint002 <@t> umn.edu (meint002) Date: Wed Mar 22 13:19:31 2006 Subject: [Histonet] DAB in tablet form Message-ID: <200603221919.k2MJJQ8t021230@vanguard.software.umn.edu> Dear Histos, Due to the hazards of working with 3,3’ diaminobenzidine tetrahydrochloride (DAB) for muscle enzyme staining, I am considering purchasing DAB as either 5mg, or 10mg tablets. Some companies have them dissolve in water and others in a buffer solution. I am looking for input from anyone who has used these tablets. Do you like them, do they work well, staining results, which company, etc.... I know that you can also get DAB in sealed separate vials, but it is much more expensive and we are in an academic research setting where cost is a consideration. I am particularly interested in using them in the Cytochrome Oxidase stain. I have tried many procedures for this stain and my Dr. is not all that pleased with the results. If anyone has a good procedure for this stain, I would be grateful if you would send me a copy. I am revamping this lab and have had a lot of questions for the histonet that you have been very helpful in addressing. Thanks for all the suggestions in advance. Joyce Meints Histologist University of Minnesota Paul and Sheila Wellstone Muscular Dystrophy Center MMC 206 420 Washington Ave. SE Minneapolis, MN 55455-0392 e-mail: meint002@umn.edu Fax: 612-625-8488 lab phone: 612-626-4703 From Thomas.Crowell <@t> biogenidec.com Wed Mar 22 13:40:35 2006 From: Thomas.Crowell <@t> biogenidec.com (Thomas Crowell) Date: Wed Mar 22 13:41:47 2006 Subject: [Histonet] Fixation sensitive IHC markers In-Reply-To: Message-ID: Dear Histonetters, Can anyone suggest an antibody that I could use on human FFPE samples that shows relative degrees of reactivity, based on variable factors such as fixation time or tissue autolysis? I would like to assess the reactivity of commercially available tissue bank samples. Any suggestions would be appreciated. Tom Crowell Biogen Idec Cambridge, MA From la.sebree <@t> hosp.wisc.edu Wed Mar 22 14:00:54 2006 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Wed Mar 22 14:01:01 2006 Subject: [Histonet] Fixation sensitive IHC markers Message-ID: Our pathologists have relied on a vimentin stain to assess the quality of fixation and processing. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Crowell Sent: Wednesday, March 22, 2006 1:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fixation sensitive IHC markers Dear Histonetters, Can anyone suggest an antibody that I could use on human FFPE samples that shows relative degrees of reactivity, based on variable factors such as fixation time or tissue autolysis? I would like to assess the reactivity of commercially available tissue bank samples. Any suggestions would be appreciated. Tom Crowell Biogen Idec Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From la.sebree <@t> hosp.wisc.edu Wed Mar 22 14:10:49 2006 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Wed Mar 22 14:10:55 2006 Subject: [Histonet] CAP - ANP.11713 Phase II Message-ID: In our lab (IHC & ISH), every case that goes out to the pathologist is accompanied by the original request form that has a "QA" form on the back. The pathologist is obligated to select "satisfactory" or "unsatisfactory" in response to the statement: "I have reviewed the patient and positive/negative control slides associated with the immunostains requested on this case and find them satisfactory/unsatisfactory" and initial his/her response. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rebecca Barnhart Sent: Wednesday, March 22, 2006 11:44 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] CAP - ANP.11713 Phase II I created a database that we enter our daily count (the date, first and last case number, the number of blocks and slides). Also there are 2 prompts to be checked by the pathologist, one for H & E and the other for cytology stain. We run a control as the first and last slide everyday. If the stain is ok he puts a check in both boxes, if there is a problem there is a spot to enter comments. I started doing it this way so that if there is a problem with the stain I can easily go back and see what cases it affected. I have a report that I can print to show the quality of stain if an inspector request it. Previously we had a sheet the pathologist kept at his desk and check each day if the stain was ok and make comments if not. I also use the spot for comments to document if we have changed something (trying a different stain, new paraffin, added time to a step on the processor or stainer, etc). So if there are questions or we need to compare slides (because of a new product or new procedure) I have a report that I run and I can easily pull the slides. I also use this database to enter all statistical information. So at any time I can give the pathologist or manager any number for anatomic pathology. >>> "Demarinis, Carolyn" 3/22/2006 11:34:14 AM >>> There is a new question on the CAP checklist: ANP.11713 - Phase II "Is there documented evidence of daily review of technical quality of histologic preparations by the pathologist?" Our present procedure is the supervisor/technologist documents daily review, but it is very clear that CAP is requiring the pathologist to initial a document daily proving they have reviewed the technical quality of slides. How are other hospitals satisfying this requirement? It seems crazy to make a pathologist initial a document daily, when it is evident they review hundreds of slides a day, and they would certainly report a problem to the supervisor when the stain is unacceptable. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Wed Mar 22 14:41:08 2006 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Wed Mar 22 14:40:51 2006 Subject: [Histonet] DAB in tablet form In-Reply-To: <200603221919.k2MJJQ8t021230@vanguard.software.umn.edu> References: <200603221919.k2MJJQ8t021230@vanguard.software.umn.edu> Message-ID: I too am in academic labs so relate to the cost concern; however, I strongly recommend DAB in liquid kit form - either DAB+ from DAKO or from Zymed. They are liquid ready to use concentrates diluted into buffer either supplied with the kit or you provide yourself. I have used both for years and years now with excellent results. Worth the extra bucks (about 80-100 dollars for 110 mls which is actually quite a lot) for consistent results time after time after time - without the hazards of working with powder or solids. Good luck! Andrea >Dear Histos, > >Due to the hazards of working with 3,3? diaminobenzidine tetrahydrochloride >(DAB) for muscle enzyme staining, I am considering purchasing DAB as either >5mg, or 10mg tablets. Some companies have them dissolve in water and others >in a buffer solution. > >I am looking for input from anyone who has used these tablets. Do you like >them, do they work well, staining results, which company, etc.... I know >that you can also get DAB in sealed separate vials, but it is much more >expensive and we are in an academic research setting where cost is a >consideration. > >I am particularly interested in using them in the Cytochrome Oxidase stain. >I have tried many procedures for this stain and my Dr. is not all that >pleased with the results. If anyone has a good procedure for this stain, I >would be grateful if you would send me a copy. > >I am revamping this lab and have had a lot of questions for the histonet that >you have been very helpful in addressing. > >Thanks for all the suggestions in advance. > > >Joyce Meints >Histologist > >University of Minnesota >Paul and Sheila Wellstone Muscular Dystrophy Center >MMC 206 >420 Washington Ave. SE >Minneapolis, MN 55455-0392 > >e-mail: meint002@umn.edu >Fax: 612-625-8488 >lab phone: 612-626-4703 -- From ploykasek <@t> phenopath.com Wed Mar 22 14:45:12 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Wed Mar 22 14:45:35 2006 Subject: [Histonet] guidelines for Her2 interpretation In-Reply-To: <80CDD9C3FEEAFD4982B114C4A6DFD00EE4A3D4@uphsmbx2.UPHS.PENNHEALTH.PRV> Message-ID: Diana, yes the standard is the Herceptest guideline, as far as I know. I haven't looked at some of the more recent FDA approved tests. I highly recommend correlating your scores with Her2 FISH results. Also, if you have >1 pathologist, the pathologists should periodically review cases together so that their eyes stay "calibrated" to the same level of staining/scoring. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA I have been out of the loop with Her- 2 for a while, and the last I > knew, it was left up to the individual labs to establish standards among > the interpreting physicians for interpretation, pretty much based on > the original Herceptest guidelines. Have any other standards been > established? If so, would someone please provide me with a reference? > > Much obliged, > Diana Goodwin > Supervisor, Anatomic Pathology > Pennsylvania Hospital > Preston 655-C > ph. 215-829-6532 > fax 215-829-7564 > e-mail goodwind@[pahosp.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From dpconsult <@t> earthlink.net Wed Mar 22 14:03:34 2006 From: dpconsult <@t> earthlink.net (Dick Paulson [Source Medical Products]) Date: Wed Mar 22 14:58:00 2006 Subject: [Histonet] p16 antibody Message-ID: Dr. Cartun, Diagnostic BioSystems can provide the p16 antibody. Clone(JC8) Isotype(IgG2a) If you have any questions, please contact me directly. Source Medical Products Dick Paulson dpconsult@earthlink.net Phone (800) 393-6345 ---- Original Message ---- We have been using Novocastra's p16 antibody (distributed by Vision BioSystems here in the USA). I have been told that their antibody is back-ordered. I know DAKO sells a p16 kit, but I'm only interested in getting the antibody. Are there any other sources for p16 that work in formalin-fixed, paraffin-embedded tissues? Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From bamoe <@t> gundluth.org Wed Mar 22 14:58:31 2006 From: bamoe <@t> gundluth.org (bamoe@gundluth.org) Date: Wed Mar 22 14:58:37 2006 Subject: [Histonet] Contaminated Alcohols from VIP processors Message-ID: Hello all - Has anyone had experience with contaminated alcohols coming off a Tissue-Tek VIP processor? We have two processors - one is a Miles VIP 3000, purchased in 1993, the other is a Sakura VIP E300 purchased in 1996. We have started experiencing the problem on both machines. Our processing schedule utilizes 2 stations each of formalin, 80% ETOH, recycled 95% ETOH, absolute ETOH (pure, not reagent grade), xylene, 3 or 4 paraffin stations, 1 cleaning xylene, 1 cleaning absolute ETOH. The formalins and 80% are not contaminated, however the 95% and absolutes show cloudiness when flushed with water down the drain. (We are assuming that it's xylene causing this, but have not done analysis to confirm.) Preventative maintenance was just done last week on both machines (by our in-house biomed personnel) and we still have the cloudiness in the 95% and absolute alcohols. We have ruled out any problems with our recycler and know that the alcohols are clean when they are put on the processor. Any comments would be greatly appreciated! Barb Moe Gundersen Lutheran Medical Center La Crosse WI bamoe@gundluth.org From gcallis <@t> montana.edu Wed Mar 22 15:27:28 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Mar 22 15:27:38 2006 Subject: Avoiding Re: [Histonet] DAB in tablet form In-Reply-To: References: <200603221919.k2MJJQ8t021230@vanguard.software.umn.edu> Message-ID: <6.0.0.22.1.20060322142207.01b3db88@gemini.msu.montana.edu> I totally agree with Andrea. Another superb two part DAB substrate kit is Pierce DAB (brown) $65 for 250 ml kit or Pierce CN/DAB (black) $78 for 250 ml kit, and you can make up as much or little as you want for number of slides in a run. Acadamic labs often get discounted pricing and free shipping other than hazardous material costs, this can be purchased through Fisher and probably VWR too. At 01:41 PM 3/22/2006, you wrote: >I too am in academic labs so relate to the cost concern; however, I >strongly recommend DAB in liquid kit form - either DAB+ from DAKO or from >Zymed. They are liquid ready to use concentrates diluted into buffer >either supplied with the kit or you provide yourself. > >I have used both for years and years now with excellent results. Worth the >extra bucks (about 80-100 dollars for 110 mls which is actually quite a >lot) for consistent results time after time after time - without the >hazards of working with powder or solids. > >Good luck! >Andrea Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From rjbuesa <@t> yahoo.com Wed Mar 22 15:30:00 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 22 15:30:07 2006 Subject: [Histonet] DAB in tablet form In-Reply-To: <200603221919.k2MJJQ8t021230@vanguard.software.umn.edu> Message-ID: <20060322213000.38655.qmail@web61221.mail.yahoo.com> Joyce: I used for years the tablets with excellent results. I prefer the tablets and to dissolve them when needed. Ren? J. meint002 wrote: Dear Histos, Due to the hazards of working with 3,3? diaminobenzidine tetrahydrochloride (DAB) for muscle enzyme staining, I am considering purchasing DAB as either 5mg, or 10mg tablets. Some companies have them dissolve in water and others in a buffer solution. I am looking for input from anyone who has used these tablets. Do you like them, do they work well, staining results, which company, etc.... I know that you can also get DAB in sealed separate vials, but it is much more expensive and we are in an academic research setting where cost is a consideration. I am particularly interested in using them in the Cytochrome Oxidase stain. I have tried many procedures for this stain and my Dr. is not all that pleased with the results. If anyone has a good procedure for this stain, I would be grateful if you would send me a copy. I am revamping this lab and have had a lot of questions for the histonet that you have been very helpful in addressing. Thanks for all the suggestions in advance. Joyce Meints Histologist University of Minnesota Paul and Sheila Wellstone Muscular Dystrophy Center MMC 206 420 Washington Ave. SE Minneapolis, MN 55455-0392 e-mail: meint002@umn.edu Fax: 612-625-8488 lab phone: 612-626-4703 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. PC-to-Phone calls for ridiculously low rates. From Charles.Embrey <@t> carle.com Wed Mar 22 15:33:19 2006 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Wed Mar 22 15:33:38 2006 Subject: [Histonet] Contaminated Alcohols from VIP processors Message-ID: I had the same problem and worked with my sales rep. I was finally told that what I was reporting was impossible. When it was verified by equipment repair, my rep told me that the bottom line was that Sakura sold me a tissue processor and it did process tissue. They made no promise that I would be able to recycle the reagents so the matter was closed. Needless to say, I replaced the VIP with a TBS unit and now have no problem recycling my alcohol. Charles Embrey PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of bamoe@gundluth.org Sent: Wednesday, March 22, 2006 2:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Contaminated Alcohols from VIP processors Hello all - Has anyone had experience with contaminated alcohols coming off a Tissue-Tek VIP processor? We have two processors - one is a Miles VIP 3000, purchased in 1993, the other is a Sakura VIP E300 purchased in 1996. We have started experiencing the problem on both machines. Our processing schedule utilizes 2 stations each of formalin, 80% ETOH, recycled 95% ETOH, absolute ETOH (pure, not reagent grade), xylene, 3 or 4 paraffin stations, 1 cleaning xylene, 1 cleaning absolute ETOH. The formalins and 80% are not contaminated, however the 95% and absolutes show cloudiness when flushed with water down the drain. (We are assuming that it's xylene causing this, but have not done analysis to confirm.) Preventative maintenance was just done last week on both machines (by our in-house biomed personnel) and we still have the cloudiness in the 95% and absolute alcohols. We have ruled out any problems with our recycler and know that the alcohols are clean when they are put on the processor. Any comments would be greatly appreciated! Barb Moe Gundersen Lutheran Medical Center La Crosse WI bamoe@gundluth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From peptolab <@t> hamptons.com Wed Mar 22 16:08:34 2006 From: peptolab <@t> hamptons.com (Jeff Silverman) Date: Wed Mar 22 16:09:01 2006 Subject: [Histonet] CAP Standard ANP.11713 - Phase II Message-ID: <000001c64dfd$297e3740$6401a8c0@jeffrey028c8d9> Previously, either myself of the lead tech for the day would examine a control slide- a tonsil or fallopian tube from the day's workload, to document proper staining and processing. To comply with this standard during our December survey, we created a daily form (yet another) distributed to whichever of our three pathologists is signing out that day. Any deficiencies or problems in a specific slide including staining issues, folds, wrinkles, or scratches, incomplete sections, misoriented tissues, floaters or contaminants, bubbles under the coverslip, mislabeling are listed by the docs. The histotech involved must address the problem and document what was done to remedy the situation. This provides a tool for continuing quality improvement so trends can be identified and individuals with chronic problems can be retrained/counseled. Jeffrey Silverman HT HTL QIHC (ASCP) Pathologists' Assistant Southside Hospital Bay Shore New York USA From Luis.Chiriboga <@t> med.nyu.edu Wed Mar 22 17:02:36 2006 From: Luis.Chiriboga <@t> med.nyu.edu (Luis Chiriboga) Date: Wed Mar 22 16:59:16 2006 Subject: [Histonet] Fixation sensitive IHC markers In-Reply-To: Message-ID: you might want to take a look at: "Assessment of antigen damage in immunohistochemistry. the Vimentin internal control" by Hector Battifora. American Journal of Clinical Pathology November 1991 96(5) 669. Hope it helps Luis ____________________________________ Luis Chiriboga Ph.D. NYU Cancer Institute and Bellevue Hospital Center New York University School Of Medicine Department Of Pathology 4W27 462 First Avenue New York, N.Y. 10016 W(212) 562-4667. F(212) 263-2041 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Thomas Crowell Sent: Wednesday, March 22, 2006 2:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fixation sensitive IHC markers Dear Histonetters, Can anyone suggest an antibody that I could use on human FFPE samples that shows relative degrees of reactivity, based on variable factors such as fixation time or tissue autolysis? I would like to assess the reactivity of commercially available tissue bank samples. Any suggestions would be appreciated. Tom Crowell Biogen Idec Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Wed Mar 22 17:20:21 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Mar 22 17:20:29 2006 Subject: [Histonet] Contaminated Alcohols from VIP processors Message-ID: <09C945920A6B654199F7A58A1D7D1FDE017176AA@lsexch.lsmaster.lifespan.org> I have noticed the same thing, and I don't think the cloudiness is due to xylene. I believe it is due to water-insoluble substances, particularly lipids, which have leached out of the tissue samples into the stronger alcohols. Because these substances are water-insoluble they don't tend to dissolve in the formalin or the weaker alcohols where a lot of water is present, but they do dissolve somewhat in the stronger alcohols. Also, because they are water-insoluble, they precipitate (become cloudy) when water is added to the alcohol, as in flushing down the drain. Because I'm in a service research lab, I often run one kind of tissue per processing run, and sometimes only one kind of tissue for several days. I have noticed that the degree of cloudiness relates not only to the number of specimens I have run since the last solvent change, but also very much to the kind of tissues that were processed. I can run kidney or lung or muscle specimens all week and there is minimal cloudiness when I discard the alcohols. But if I process a large number of skin or breast or colon or other fatty samples, the alcohol turns milky white when it hits the water. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > bamoe@gundluth.org > Sent: Wednesday, March 22, 2006 12:58 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Contaminated Alcohols from VIP processors > > > > > > Hello all - > > Has anyone had experience with contaminated alcohols coming off a > Tissue-Tek VIP processor? > > We have two processors - one is a Miles VIP 3000, purchased in 1993, the > other is a Sakura VIP E300 purchased in 1996. > We have started experiencing the problem on both machines. > > Our processing schedule utilizes 2 stations each of formalin, 80% ETOH, > recycled 95% ETOH, absolute ETOH (pure, not reagent grade), xylene, 3 or 4 > paraffin stations, 1 cleaning xylene, 1 cleaning absolute ETOH. > > The formalins and 80% are not contaminated, however the 95% and absolutes > show cloudiness when flushed with water down the drain. (We are assuming > that it's xylene causing this, but have not done analysis to confirm.) > > Preventative maintenance was just done last week on both machines (by our > in-house biomed personnel) and we still have the cloudiness in the 95% and > absolute alcohols. > > We have ruled out any problems with our recycler and know that the > alcohols > are clean when they are put on the processor. > > Any comments would be greatly appreciated! > > Barb Moe > Gundersen Lutheran Medical Center > La Crosse WI > bamoe@gundluth.org > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From nellisk <@t> mail.nih.gov Wed Mar 22 17:25:57 2006 From: nellisk <@t> mail.nih.gov (Nellis, Kevin Lee (NIH/NCI) [E]) Date: Wed Mar 22 17:26:04 2006 Subject: [Histonet] National Search for Histology Supervisor at the National Cancer Institute; up to $84,559 per year Message-ID: Dear Collegues: The NCI Laboratory of Pathology is are actively recruting for a Histology Supervisor position. The job announcement is available on the www.USAJobs.gov website. The position number is NCI-06-116352. You may review the full details at: http://jobsearch.usajobs.opm.gov/getjob.asp?JobID=41029650 There are three different government salary ranges for this position based on the level of experience of the candidate. Please see the full positing for details for examples of specialized experience. If a GS-10 level candidate is selected, the selected individual will be placed in a Career Development Program. A successful candidate can be promoted to the full level of a GS-12, during his/her career. The salary ranges including locality pay are: GS-10, Salary range $ 49,397 - $ 64,213 GS-11, Salary range $ 54,272 - $ 70,558 GS-12, Salary range $ 65,048 - $ 84,559 RELOCATION EXPENSES AND RECRUITMENT BONUS MAY BE PAID. Interested parties may apply online at on the www.USAJobs.gov website or you send an application directly to Ernesto Corrales. Be sure to read the announcement carefully and supply all the information. Applicants MUST submit a supplemental statement to their resume or application addressing each of the specific KSAs. Only the most highly qualified candidates will be referred to the hiring manager. The application deadline is Tuesday, April 11, 2006. For information about the NIH and surrounding areas, please see www.nih.gov For information about benefits and awards please see: http://hr.od.nih.gov/default.htm Thank you for your interest. Please forward this e-mail to anyone that might consider this exciting opportunity. Kevin L. Nellis, M.S., M.T. (A.S.C.P.) Clinical Laboratory Manager Laboratory of Pathology Center for Cancer Research National Cancer Institute National Institutes of Health U.S. Department of Health and Human Services 10 Center Drive, Room 2A33, MSC 1500 Bethesda, MD 20892 OFFICE: (301) 594-9532 FAX: (301) 402-0043 http://home.ncifcrf.gov/ccr/lop/Clinical/default.asp From JMacDonald <@t> mtsac.edu Thu Mar 23 00:12:57 2006 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Mar 23 00:13:18 2006 Subject: [Histonet] Training Med Techs - some candid comments Message-ID: Dear Mr. Kirby, I do not usually get involved in the mu but your response has changed that. Your Becky and to all Histotechs. Becky was not ins interpretation is that she is frustrated with Hi overlooked for management positions in favor of Med Techs.&n train a Med Tech with no histology experience if you have a very c apable Histotech available? She did not mean to imply that Histotechn meant that o train somebody on t correct me if I am wrong. As a Med Tech, I have worked in all areas of those fancy biochemistry and hematology analyzers, as matching blood for an ER patient. I know what stress i work in histology where things can also go wrong and depend the laboratory the turn-around-time expectations can be unreasonable . What we do in Histology can also impact a patient's health. A lthough the Pathologist makes the diagnosis, it is made on slides that we h grievous pr your ability hands throughout < gruelin Techs. Many h There are many of us wh considered a boring "dead end division" field because we truly enjoy wh the respect we get from people like Jennifer MacDonald -----histonet-bounces@lists.utsouthwestern.edu wrote To: "Histonet \(E-mail\)" Fro Sent by: histonet-bounces@ Date: 03/22/2006 05:57AM Subject: [Histonet] Dear Ms Orr. You ma one! Cli Your comment " Training a MT to be train a policeman to be a fireman" rather s I would like to follow with a counter comment, "C can be shown how to the flames. Granted, His requiring fine manual dext better than operating a high output Bi analyser or cross-matching a pint of blood, where a can kill a patient. Plus you operate under a fraction of the stress we are subjected to - try working for a full day, and then doi expected to report f It's a gross insult to insinuate that ours is a "career". As students, we spent 5 - 6 mo was available in the lab - Chempath, Ha Cyto, Immunology, Human genetics, blood transfusio even Histopath. When we passed our finals, we were allowed to c hoose which discipline we wanted to further our careers, and each year, wit Histopath, as it was cons (Yes, pun intended). As fo Dept, then you c systems apply, regardless o practical applications of the work in hand Histopathology is just one of the services in the medi you don't walk on water, and neither do we. As far as I am conce rned, once trained as a general Med Tech, you can be trained in virtually a thumbs" me, who after 35 half decent section or make a passa good as a manager!) End of tirade - climb into bunker, to await the verbal unleashed................ Mr.M.Kirby J South Africa -----Original Message----- Fr [[1]mailto:histonet-bounces@lists.utsou Of Orr, Rebecca Sent: 17 March 2006 17:04 To Cc: &n Subject: [Histonet] Training Med Techs H I would appreciate any feedback from those of you who may to train MT's (ASCP) to work in Histology. They would be train them into Anatomic Patho Candid comments welcome, especial histology! To me it would be like trying to fireman, it's a career, not a job, right? We see a HT shortage in the Chicago area, but I am unsure how to addre Degreed individuals have proven critical thinkin traditional education pathway, so I see the advantages, but ignore very capable HT managers with proven management and organizationa an issue with me.I mean it's not like Non degreed HT's are stooopid or something. < Becky Becky Orr CLA,HT(ASCP) IHC Lead Eva 847-570-2771 ____ ______________________ 5F__ Histonet mailing Histonet@lists.utsouthwestern.edu [2]http://lists.utsou ************************ ********************************************************** The views exp those of the author and Laboratory Services or its management. The e-mail is confidential and is intended solely for the Access to this e-mail by anyone else is unauthorized. If you not the intended recipient, any disclosure, copying, distribution or an and may be unl Whilst all reasonable steps are taken to ensure the accuracy and integrity of information and data transmitted electronically and to preserv responsibility whatsoever is for whatever reason, corrupted or does destination. ******************************* **************************************************** _ ______________________ 5F__ _____________________ Histonet Histonet@lists.utsouthwestern.edu [3]http://li References 1. 3D"mailto:histonet-bounces@li 2. 3D"http://lists.utsout=/ 3. 3D"http://lis=/ From JEllin <@t> yumaregional.org Thu Mar 23 06:34:29 2006 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Thu Mar 23 06:34:48 2006 Subject: [Histonet] MAK 6 HELP PLEASE!!!!!!!!!!! Message-ID: Hello everyone I need some really big help we are trying to add an additional antibody to our system MAK6 from Zymed. We are using Ventana stainers, one is a Benchmark the other is LT. If anyone out there is using this antibody on these stainers and having success, PLEASE!!!!!!!!!!!! let me have your protocol for pariffin embeded and formalin fixed tissue. Jesus Ellin Yuma Regional Medical Center From iatomatt <@t> yahoo.co.uk Thu Mar 23 09:00:04 2006 From: iatomatt <@t> yahoo.co.uk (MattG) Date: Thu Mar 23 09:00:08 2006 Subject: [Histonet] Fw: Thermo/ Leica Message-ID: <001501c64e8a$785f4240$6501a8c0@acer311vpbceh0> Does anyone have a current email address for Pauline Fisher of Thermo (formerly Shandon)? I'd also be grateful if someone could send me an electronic version of the user manual for the Leica TP1050. If there are any UK based Leica or Thermo reps on here, could one of you please drop me an email as I have a couple of questions I'd like to ask. Thanks, Matt Griffiths ___________________________________________________________ Win a BlackBerry device from O2 with Yahoo!. Enter now. http://www.yahoo.co.uk/blackberry From Janet.Bonner <@t> FLHOSP.ORG Thu Mar 23 09:34:15 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Thu Mar 23 09:38:26 2006 Subject: [Histonet] Contaminated Alcohols from VIP processors References: Message-ID: I experienced this problem before and discovered the 95% ETOH was the culprit ( the 100%s could be white due to the 95% carried over into them). The company is going to deny this of course, but our supplier later came back to say there was a manufacturing problem that was not picked up on in their QC. Try another brand before you do anything, or call your supplier to ask if there are any other reported problems. -Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of bamoe@gundluth.org Sent: Wed 3/22/2006 3:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Contaminated Alcohols from VIP processors Hello all - Has anyone had experience with contaminated alcohols coming off a Tissue-Tek VIP processor? We have two processors - one is a Miles VIP 3000, purchased in 1993, the other is a Sakura VIP E300 purchased in 1996. We have started experiencing the problem on both machines. Our processing schedule utilizes 2 stations each of formalin, 80% ETOH, recycled 95% ETOH, absolute ETOH (pure, not reagent grade), xylene, 3 or 4 paraffin stations, 1 cleaning xylene, 1 cleaning absolute ETOH. The formalins and 80% are not contaminated, however the 95% and absolutes show cloudiness when flushed with water down the drain. (We are assuming that it's xylene causing this, but have not done analysis to confirm.) Preventative maintenance was just done last week on both machines (by our in-house biomed personnel) and we still have the cloudiness in the 95% and absolute alcohols. We have ruled out any problems with our recycler and know that the alcohols are clean when they are put on the processor. Any comments would be greatly appreciated! Barb Moe Gundersen Lutheran Medical Center La Crosse WI bamoe@gundluth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mprice26 <@t> juno.com Thu Mar 23 10:11:00 2006 From: mprice26 <@t> juno.com (mprice26@juno.com) Date: Thu Mar 23 10:11:53 2006 Subject: [Histonet] RE:JOB DESCRIPTION FOR HISTOLOGY ASSISTANT Message-ID: <20060323.081133.783.179@webmail01.nyc.untd.com> HI HISTONETTERS, Can someone be so kind as to share a job description with me for a histology dept.assistant/aide (Non Registered). Thank you. Marsha Price From kbradshaw <@t> lcpath.com Thu Mar 23 10:24:09 2006 From: kbradshaw <@t> lcpath.com (Kari Bradshaw) Date: Thu Mar 23 10:24:18 2006 Subject: [Histonet] RE:JOB DESCRIPTION FOR HISTOLOGY ASSISTANT In-Reply-To: <20060323.081133.783.179@webmail01.nyc.untd.com> Message-ID: <200603231624.k2NGOC122950@plus34.host4u.net> POSITION: HISTOLOGY ASSISTANT SUPERVISOR: Histology Supervisor DEPARTMENT: Histology JOB SUMMARY Performs histology preparation, entry and recording functions in accordance with departmental procedures and policies. NATURE AND SCOPE Reports directly to the histology supervisor. Requires patient contact when obtaining specimens in a high stress environment for the patient because of the nature of the test. Extensive contact with clients, laboratory and hospital personnel is experienced in person, by phone or in written communication. This position description includes only essential functions of the position. This in no way states or implies that these are the only duties to be performed by the incumbent. The incumbent may be required to follow other job related instruction and to perform other job-related duties requested by the supervisor. ESSENTIAL FUNCTIONS Specimen Preparation . Accession specimens . Coverslip and label slides following guidelines outlined in the Histology Procedure Manual. . Maintain a clean and safe prep work area according to LCP Infection/Exposure Control and Chemical Hygiene guidelines. . Assist pathologist and/or histotechnologist with specimen grossing. Supply And Maintenance Responsibilities . Prepares and labels necessary stains and reagents according to procedures outlined in the procedure manual. . Maintains record keeping logs according to policy manual guidelines. . Maintains equipment according to company procedures Computer Responsibilities . Enters and prints necessary labels. . Prints necessary patient history information Support Responsibilities . Files histology requisitions and slides . Fills client supply orders . Wash and maintain glass and plastic ware . Ensures proper disposal of wet tissue and chemicals as per company policy. . May assist with the orientation and training of other employees. . Maintains confidentiality regarding all phases of laboratory work and findings. MINIMUM QUALIFICATIONS . High school diploma or equivalent . Demonstrate accurate attention to detail PHYSICAL DEMANDS AND WORKING ENVIRONMENT This position requires mental agility and physical dexterity along with the ability to follow through with often times mundane tasks. Attention to detail is required. Must be able to exercise patience and accuracy in the face of multiple interruptions. Observes strict safety precautions when handling biological specimens and chemicals. Employee Signature Supervisor Signature -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mprice26@juno.com Sent: Thursday, March 23, 2006 8:11 AM To: HISTONET@lists.utsouthwestern.edu Subject: [Histonet] RE:JOB DESCRIPTION FOR HISTOLOGY ASSISTANT HI HISTONETTERS, Can someone be so kind as to share a job description with me for a histology dept.assistant/aide (Non Registered). Thank you. Marsha Price _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Thu Mar 23 10:54:41 2006 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Thu Mar 23 10:54:46 2006 Subject: [Histonet] P16 antibody Message-ID: <0E394B648E5284478A6CCB78E5AFDA270171FCF6@hpes1.HealthPartners.int> We currently use Clone 16P04 from Cell Marque and have great results with it!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From HParker <@t> Skaggs.Net Thu Mar 23 11:14:15 2006 From: HParker <@t> Skaggs.Net (Parker, Helayne) Date: Thu Mar 23 11:14:45 2006 Subject: [Histonet] CAP Guidelines for Microwaves Message-ID: <2FABC6145388F24EBA8CBD6EC165BCCB02AFBA27@mail1-schc.skaggs.net> I was wondering if anyone new what CAP require of us if we have a common household microwave that we use for special stains. Helayne Parker,HT (ASCP) Histology Section Head Skaggs Community Health Center Branson, Missouri From jkiernan <@t> uwo.ca Thu Mar 23 11:15:27 2006 From: jkiernan <@t> uwo.ca (John A. Kiernan) Date: Thu Mar 23 11:15:28 2006 Subject: [Histonet] oysters larvae again References: <09C945920A6B654199F7A58A1D7D1FDE017176A4@lsexch.lsmaster.lifespan.org> Message-ID: <4422D7AF.46AE7D26@uwo.ca> Anne Sophie's MEMPFA-T fixative (0,1 M Mops pH 7,4; 2 mM EGTA; 1 mM MgSO4; 4% paraformaldehyde; 0,1% Tween 20) does contain EGTA, which chelates calcium ions, but 2mm seems intuitively to be very weak. Are the shells of oyster larvae extremely thin? I've not come across this MEMPFA-T fixative before. Could anyone provide a reference for it? -- ------------------------------- John A. Kiernan Department of Anatomy and Cell Biology The University of Western Ontario London, Canada N6A 5C1 kiernan[AT]uwo.ca http://publish.uwo.ca/~jkiernan/ http://instruct.uwo.ca/anatomy/530/index.htm _______________________________ "Monfils, Paul" wrote: > > I don't have a background in ISH, so I can't offer an opinion on the best > fixative for that purpose. > > Regarding your second question, while I'm not familiar with either fixative, > I can see that the Davidson fixative, containing 10% acetic acid and no > buffers, would decalcify (dissolve) larval oyster shells. But the MEMPFA-T > appears to be made up in pH 7.4 buffer, in which case I don't see how it > would decalcify. In any case, I am wondering why you are using a > formaldehyde-based fixative for TEM? Ordinarily a glutaraldehyde-based > fixative would be preferable for TEM. > > > ---------- > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > > Anne-Sophie MARTINEZ > > Sent: Tuesday, March 21, 2006 5:13 AM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] oysters larvae again > > > > Hello, > > (1) I plan to fix oysters larvae/juveniles (4 month) for in toto ISH. > > Which fixative is the best : Davidson (10% formaldehyde 37-40%, 30% > > ethanol 95%, 60% seawater, 10% acetic acid) or MEMPFA-T (0,1 M Mops pH > > 7,4; 2 mM EGTA; 1 mM MgSO4; 4% paraformaldehyde; 0,1% Tween 20)? > > (2) Would it be possible to use the MEMPFA-T to fix and "decalcify" the > > shelves of the juveniles for TEM (embedding in type lowyril, unicril, > > LR-white resins) instead of classical fixative (4% PFA) and then EDTA? > > Thank you very much again. > > Anne-Sophie > > > > From tpmorken <@t> labvision.com Thu Mar 23 11:26:43 2006 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Thu Mar 23 11:26:48 2006 Subject: [Histonet] RE: Training med techs - some candid comments Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA20328D778@usca0082k08.labvision.apogent.com> M. Kirby wrote: "Plus you operate under a fraction of the stress we [med techs] are subjected to..." This made me a laugh - M. Kirby has obviously never had to spend hours cutting dozens of frozens sections as fast as possible while the patient is on the table and the surgeons and pathologists breath heaviliy over his shoulder in anticipation! That aside, he does highlight one point - in the system he works in, South Africa med techs went through histopath training during med tech training. Much of the world does it this way. The US does not, and has not for several decades. So he is advocating from a very different viewpoint than those of us in the US. While he is correct that a paper-pusher is a paper-pusher no matter where they push their paper, to be a really effective manager of a small department, it is best to have extensive practical experience in that department. I have seen some institutions handle this is different ways if a suitable paper-pusher is not available among the histology staff - usually it involves giving the paper to be pushed to a med tech who has left the bench and has been highly trained in paper-pushing and leaving the technical aspects of the job to the experts. Tim Morken From rjbuesa <@t> yahoo.com Thu Mar 23 11:35:25 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 23 11:35:30 2006 Subject: [Histonet] CAP Guidelines for Microwaves In-Reply-To: <2FABC6145388F24EBA8CBD6EC165BCCB02AFBA27@mail1-schc.skaggs.net> Message-ID: <20060323173525.60019.qmail@web61217.mail.yahoo.com> Helayne: In short, the message from CAP is: "do not use them!" Unfortunate though! Ren? J. "Parker, Helayne" wrote: I was wondering if anyone new what CAP require of us if we have a common household microwave that we use for special stains. Helayne Parker,HT (ASCP) Histology Section Head Skaggs Community Health Center Branson, Missouri _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Blab-away for as little as 1?/min. Make PC-to-Phone Calls using Yahoo! Messenger with Voice. From peptolab <@t> hamptons.com Thu Mar 23 12:54:19 2006 From: peptolab <@t> hamptons.com (peptolab) Date: Thu Mar 23 12:46:07 2006 Subject: [Histonet] (no subject) Message-ID: <380-22006342318541925@hamptons.com> Barb, You did not mention- how are the tissues?? Cloudiness of 95 and absolute when washing down the drain is perfectly normal and is due to all the dissolved fat from your specimens. Jeff Silverman Southside Hospital From amdomenick <@t> yahoo.com Thu Mar 23 13:33:34 2006 From: amdomenick <@t> yahoo.com (Angie Domenick) Date: Thu Mar 23 13:33:40 2006 Subject: [Histonet] Control block and slide management In-Reply-To: Message-ID: <20060323193334.5023.qmail@web31405.mail.mud.yahoo.com> We write on a log sheet when we notice a particular set of control slides is getting low. The person who notices there are only about 10 or so slides left in the box writes down on the log the name of the control needed, the date, how many are left and their initials. Then as someone has some down time during the day, they can cut a new batch. They write down on the log how many slides they cut and the date they were done along with their initials. As for control blocks, there are only a few of us who look up old cases for new controls when we start running out of blocks. We don't really have a system but we try to keep several blocks for each control in a box in the numerical order that corresponds to our antibody and special stain list. Angie Domenick --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. From Jackie.O'Connor <@t> abbott.com Thu Mar 23 15:01:07 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Mar 23 15:01:31 2006 Subject: [Histonet] Santa Cruz anti mouse CD31 Message-ID: Has anyone had any luck with the newest lots of Santa Cruz PECAM-1 SC1506 in FFPE murine tissue? Jackie O' From cquinlan <@t> wlgore.com Thu Mar 23 15:22:50 2006 From: cquinlan <@t> wlgore.com (Christie M Quinlan) Date: Thu Mar 23 15:22:58 2006 Subject: [Histonet] collagen I & III immuno doublestaining Message-ID: Hi all, Has anyone had any experience using collagen I and III antibodies for doublestaining on human tissue? If so, can you recommend the vendor or vendors that you had successful results with. Thanks in advance, Christie Quinlan W.L.Gore & Assoc. 4100 W. Kiltie Ln. Flagstaff, AZ 86001 928-213-1293 cquinlan@wlgore.com From emerald_lake77 <@t> yahoo.com Thu Mar 23 15:40:28 2006 From: emerald_lake77 <@t> yahoo.com (GT Hebert) Date: Thu Mar 23 15:40:35 2006 Subject: [Histonet] Santa Cruz anti mouse CD31 In-Reply-To: Message-ID: <20060323214028.8976.qmail@web31715.mail.mud.yahoo.com> Jackie, I have made a number of attempts, but without luck. The staining pattern just isn't what is should be (or better said, what it used to be). Santa Cruz sent me a free sample from there newest batch. I will keep Histonet posted if things change for the better. Gustave Gustave T. Hebert Scientist II Cardiovascular and Metabolic Diseases Wyeth Research Cambridge MA Jackie M O'Connor wrote: Has anyone had any luck with the newest lots of Santa Cruz PECAM-1 SC1506 in FFPE murine tissue? Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- New Yahoo! Messenger with Voice. Call regular phones from your PC for low, low rates. From jnocito <@t> pathreflab.com Thu Mar 23 15:51:19 2006 From: jnocito <@t> pathreflab.com (Joe Nocito) Date: Thu Mar 23 15:49:28 2006 Subject: [Histonet] CAP Guidelines for Microwaves In-Reply-To: <20060323173525.60019.qmail@web61217.mail.yahoo.com> Message-ID: I don't believe CAP is saying not to use them. They are saying use them safely with ventilation and make sure they don't leak radiation. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, March 23, 2006 11:35 AM To: Parker, Helayne; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] CAP Guidelines for Microwaves Helayne: In short, the message from CAP is: "do not use them!" Unfortunate though! Ren? J. "Parker, Helayne" wrote: I was wondering if anyone new what CAP require of us if we have a common household microwave that we use for special stains. Helayne Parker,HT (ASCP) Histology Section Head Skaggs Community Health Center Branson, Missouri _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Blab-away for as little as 1?/min. Make PC-to-Phone Calls using Yahoo! Messenger with Voice. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Mar 23 16:19:11 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Mar 23 16:19:14 2006 Subject: [Histonet] CAP Guidelines for Microwaves Message-ID: <20060323221911.90980.qmail@web61223.mail.yahoo.com> Joe: It is not external ventilation to the MW oven what CAP wants, is a vent connected from the microwave to an outlet and there is not a single household MW oven with that characteristec so, for all practical purposes the mensage keeps being: don't use them (the household appliance) for histology work, specially if tissue processing is the objective of the use. Ren? J. Joe Nocito wrote: I don't believe CAP is saying not to use them. They are saying use them safely with ventilation and make sure they don't leak radiation. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, March 23, 2006 11:35 AM To: Parker, Helayne; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] CAP Guidelines for Microwaves Helayne: In short, the message from CAP is: "do not use them!" Unfortunate though! Ren? J. "Parker, Helayne" wrote: I was wondering if anyone new what CAP require of us if we have a common household microwave that we use for special stains. Helayne Parker,HT (ASCP) Histology Section Head Skaggs Community Health Center Branson, Missouri _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Blab-away for as little as 1?/min. Make PC-to-Phone Calls using Yahoo! Messenger with Voice. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Blab-away for as little as 1?/min. Make PC-to-Phone Calls using Yahoo! Messenger with Voice. From jwatson <@t> gnf.org Thu Mar 23 16:28:52 2006 From: jwatson <@t> gnf.org (James Watson) Date: Thu Mar 23 16:29:06 2006 Subject: [Histonet] Santa Cruz anti mouse CD31 Message-ID: What lot numbers are you working with? And at what concentrations? I have been using lot # A2605 and have gotten staining, but they have not given as strong a signal as I would like. James Watson HT, ASCP Facilities Manager of Histology GNF, Genomics Institute of the Novartis Research Foundation Room C015 858-332-4647 jwatson@gnf.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Thursday, March 23, 2006 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Santa Cruz anti mouse CD31 Has anyone had any luck with the newest lots of Santa Cruz PECAM-1 SC1506 in FFPE murine tissue? Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Thu Mar 23 16:38:27 2006 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Mar 23 16:38:37 2006 Subject: [Histonet] collagen I & III immuno doublestaining In-Reply-To: Message-ID: <000001c64eca$80d01a40$0300a8c0@Chlipala> Christie We have stained for both collagen I and III on human dermis samples, are antibodies come from Biogenesis. They are both rabbit polyclonals, but we have never tried double staining. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Christie M Quinlan Sent: Thursday, March 23, 2006 2:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] collagen I & III immuno doublestaining Hi all, Has anyone had any experience using collagen I and III antibodies for doublestaining on human tissue? If so, can you recommend the vendor or vendors that you had successful results with. Thanks in advance, Christie Quinlan W.L.Gore & Assoc. 4100 W. Kiltie Ln. Flagstaff, AZ 86001 928-213-1293 cquinlan@wlgore.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1.1456 (20060323) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From mbmphoto <@t> gmail.com Thu Mar 23 09:46:31 2006 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Thu Mar 23 17:25:50 2006 Subject: [Histonet] colored OC T media - not TBS Message-ID: <0917AA63-EEDD-4543-9BAA-622E4A41044C@gmail.com> Does anyone know where I can buy "colored" OCT freezing media that is NOT TBS media? Many thanks. Maria Bartola Mejia From jwatson <@t> gnf.org Thu Mar 23 18:24:44 2006 From: jwatson <@t> gnf.org (James Watson) Date: Thu Mar 23 18:24:54 2006 Subject: [Histonet] San Diego Chapter of the California Society for Histotechnology Message-ID: We are restarting the San Diego Chapter of the California Society of Histotechnology. March 30th we will be having our second meeting at the Genomics Institute of the Novartis Research Foundation (GNF) in La Jolla at 5:30. Our first meeting was sponsored by Kevin Kirwan of McBain Instruments (thank you for dinner) and the second meeting will be sponsored by John Stachacz of Thermo Electron Corporation. You can visit the S.D. Chapter yahoo group page at http://groups.yahoo.com/group/sdhisto/ . We are looking for ideas for discussions/seminars that will interest both the clinical and research histology technicians. At the first meeting ideas like visiting the labs at the zoo and/or wild animal park, Microscopic Anatomy, Safety, Differences in Research from Clinical labs, and regulatory things were discussed. If you are interested in becoming involved, attending, sponsoring a meeting, giving a seminar, or just meeting fellow histo techs; please e-mail Donna Harclerode at dharclerode@cytoritx.com or myself at jwatson@gnf.org and join the chapter.. Come to the meeting on March 30th, have dinner, and meet your fellow San Diego area histo techs. We will be discussing future plans of the chapter and having an informal discussion about the histology lab and the work done here at the GNF histology lab for both the drug discovery and genomics departments. Please E-mail Donna or myself if you plan on attending so we can get enough food for everyone and for directions. James Watson HT, ASCP Facilities Manager of Histology GNF, Genomics Institute of the Novartis Research Foundation Room C015 858-332-4647 jwatson@gnf.org From jnocito <@t> satx.rr.com Thu Mar 23 18:42:19 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Mar 24 00:07:19 2006 Subject: [Histonet] CAP Guidelines for Microwaves References: <20060323221911.90980.qmail@web61223.mail.yahoo.com> Message-ID: <00e901c64f09$1497f200$e8bd0b43@yourxhtr8hvc4p> but what about using a table top fume absorber with charcoal filters? CAP writes these questions so ambiguously that it becomes a joke. This is the main reason why I have a negative attitude towards CAP. Joe ----- Original Message ----- From: "Rene J Buesa" To: "Joe Nocito" ; "'Parker, Helayne'" ; Sent: Thursday, March 23, 2006 4:19 PM Subject: RE: [Histonet] CAP Guidelines for Microwaves > Joe: > It is not external ventilation to the MW oven what CAP wants, is a vent > connected from the microwave to an outlet and there is not a single > household MW oven with that characteristec so, for all practical purposes > the mensage keeps being: don't use them (the household appliance) for > histology work, specially if tissue processing is the objective of the > use. > Ren? J. > > Joe Nocito wrote: > I don't believe CAP is saying not to use them. They are saying use them > safely with ventilation and make sure they don't leak radiation. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J > Buesa > Sent: Thursday, March 23, 2006 11:35 AM > To: Parker, Helayne; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] CAP Guidelines for Microwaves > > Helayne: > In short, the message from CAP is: "do not use them!" > Unfortunate though! > Ren? J. > > "Parker, Helayne" wrote: > I was wondering if anyone new what CAP require of us if we have a common > household microwave that we use for special stains. > > Helayne Parker,HT (ASCP) > Histology Section Head > Skaggs Community Health Center > Branson, Missouri > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Blab-away for as little as 1?/min. Make PC-to-Phone Calls using Yahoo! > Messenger with Voice. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > --------------------------------- > Blab-away for as little as 1?/min. Make PC-to-Phone Calls using Yahoo! > Messenger with Voice. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.385 / Virus Database: 268.3.0/290 - Release Date: 3/23/2006 > > From lpwenk <@t> sbcglobal.net Fri Mar 24 03:29:29 2006 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri Mar 24 03:30:42 2006 Subject: [Histonet] Training Med Techs - some candid comments In-Reply-To: <3F500F8B416C554EBB21FF16642F72E959CE02@marxchg03.mar.med.navy.mil> Message-ID: <004401c64f25$74044920$0739d445@HPPav2> Another way to "build it" is to build more histotech programs in the US. In the early 1980's (pre-DRG), there were 50 accredited HT/HTL programs. As of today, there are 24. If anyone is interested, I'm presenting a workshop on how to create an accredited HT/HTL program at the NSH Symposium in Phoenix, AZ on Sunday September 10, from 1-4:30 pm, workshop #28. I'll talk about working with community colleges and other hospitals/labs, to ease the burden of one hospital doing it all. The tentative schedule can be found on the NSH webpage. http://www.nsh.org/conventions/program2006.html Final version and registration in April. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DDDeltour@mar.med.navy.mil Sent: Wednesday, March 22, 2006 10:32 AM To: mike.kirby@nhls.ac.za; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Training Med Techs - some candid comments Relax Mike. I think you missed the point completely. Becky did not disrespect the MT community. She is frustrated with the lack of good Histology techs in her area. Before histology schools, the only way to get into histology was to be a lab worker (med tech) and stumble into it. That practice is not recommended/preferred anymore. It is not because med techs are not capable to learn, but because histology has advanced where specialized training is needed for the individual. To Becky, I would say "build it and they will come". First realize that the shortage is not just in your area. You have to go above and beyond to recruit people to your area/facility. Try to work with your HR department (I know good luck) and compare the benefits, wages, and bonuses to other facilities in your area. After you have tried that compare your facility to the rest of the US. It may help if you can toss in a space heater too. Chicago is way cold. :) Douglas D. Deltour HT(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mike Kirby Sent: Wednesday, March 22, 2006 8:57 AM To: Histonet (E-mail) Subject: [Histonet] Training Med Techs - some candid comments Dear Ms Orr. You may not have wanted a fight on your hands but now you've got one! Climbs on soapbox and starts tirade! Your comment " Training a MT to be a Histo Tech is like trying to train a policeman to be a fireman" rather sticks in the craw, and I would like to follow with a counter comment, "Can a Histo Tech be trained to be a fireman?" as anyone can be shown how to connect a hose to a hydrant and point it at the flames. Granted, Histopatholgy is very much a "hands on" profession, requiring fine manual dexterity and concentration, but it's no better than operating a high output Biochemistry/ Haematology analyser or cross-matching a pint of blood, where a wrong result can kill a patient. Plus you operate under a fraction of the stress we are subjected to - try working for a full day, and then doing another 13 hours of call out duty, and then you are expected to report for normal duty next day! It's a gross insult to insinuate that ours is a "job" while yours is a "career". As students, we spent 5 - 6 months working in every division that was available in the lab - Chempath, Haem, Parasitology, Micro, Cyto, Immunology, Human genetics, blood transfusion, and yes, even Histopath. When we passed our finals, we were allowed to choose which discipline we wanted to further our careers, and each year, without fail, the majority would choose anything but Histopath, as it was considered a boring "dead end division" (Yes, pun intended). As for management positions, if you can run a Chempath or Micro Dept, then you can run a Histopath Dept, as the same managerial systems apply, regardless of the discipline, it's just the practical applications of the work in hand that differ. Histopathology is just one of the services in the medical world, you don't walk on water, and neither do we. As far as I am concerned, once trained as a general Med Tech, you can be trained in virtually any other discipline, unless you are like "two left thumbs" me, who after 35 years on the bench, still cannot cut a half decent section or make a passable blood smear. (But I am good as a manager!) End of tirade - climbs off soapbox, puts on helmet, and climbs into bunker, to await the verbal barrage that's about to be unleashed................ Mr.M.Kirby Johannesburg South Africa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Orr, Rebecca Sent: 17 March 2006 17:04 To: histonet@lists.utsouthwestern.edu Cc: Delk, Linda Subject: [Histonet] Training Med Techs Hello everyone. I would appreciate any feedback from those of you who may have had to train MT's (ASCP) to work in Histology. They would be trained as histo techs with the intent to promote them into Anatomic Pathology (Histology) management positions. Candid comments welcome, especially from MT's who now work in histology! To me it would be like trying to train a policeman to be a fireman, it's a career, not a job, right? We see a HT shortage in the Chicago area, but I am unsure how to address this. Degreed individuals have proven critical thinking skills via a traditional education pathway, so I see the advantages, but to ignore very capable HT managers with proven management and organizational skills via non traditional pathways is becoming an issue with me. I mean it's not like Non degreed HT's are stooopid or something. Thank you Becky Becky Orr CLA,HT(ASCP) IHC Lead Evanston Northwestern Healthcare 847-570-2771 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet **************************************************************************** ****** The views expressed in this email are, unless otherwise stated, those of the author and not those of the National Health Laboratory Services or its management. The information in this e-mail is confidential and is intended solely for the addressee. Access to this e-mail by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken or omitted in reliance on this, is prohibited and may be unlawful. Whilst all reasonable steps are taken to ensure the accuracy and integrity of information and data transmitted electronically and to preserve the confidentiality thereof, no liability or responsibility whatsoever is accepted if information or data is, for whatever reason, corrupted or does not reach its intended destination. **************************************************************************** ******* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Fri Mar 24 04:47:18 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Fri Mar 24 04:47:26 2006 Subject: [Histonet] CAP Guidelines for Microwaves Message-ID: I also thought that it had to do with combustibility.....that the laboratory-grade microwaves were safer in the event of an explosive incident. Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, March 23, 2006 7:42 PM To: Rene J Buesa; Joe Nocito; 'Parker, Helayne'; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] CAP Guidelines for Microwaves but what about using a table top fume absorber with charcoal filters? CAP writes these questions so ambiguously that it becomes a joke. This is the main reason why I have a negative attitude towards CAP. Joe ----- Original Message ----- From: "Rene J Buesa" To: "Joe Nocito" ; "'Parker, Helayne'" ; Sent: Thursday, March 23, 2006 4:19 PM Subject: RE: [Histonet] CAP Guidelines for Microwaves > Joe: > It is not external ventilation to the MW oven what CAP wants, is a vent > connected from the microwave to an outlet and there is not a single > household MW oven with that characteristec so, for all practical purposes > the mensage keeps being: don't use them (the household appliance) for > histology work, specially if tissue processing is the objective of the > use. > Ren? J. > > Joe Nocito wrote: > I don't believe CAP is saying not to use them. They are saying use them > safely with ventilation and make sure they don't leak radiation. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J > Buesa > Sent: Thursday, March 23, 2006 11:35 AM > To: Parker, Helayne; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] CAP Guidelines for Microwaves > > Helayne: > In short, the message from CAP is: "do not use them!" > Unfortunate though! > Ren? J. > > "Parker, Helayne" wrote: > I was wondering if anyone new what CAP require of us if we have a common > household microwave that we use for special stains. > > Helayne Parker,HT (ASCP) > Histology Section Head > Skaggs Community Health Center > Branson, Missouri > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Blab-away for as little as 1?/min. Make PC-to-Phone Calls using Yahoo! > Messenger with Voice. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > --------------------------------- > Blab-away for as little as 1?/min. Make PC-to-Phone Calls using Yahoo! > Messenger with Voice. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.385 / Virus Database: 268.3.0/290 - Release Date: 3/23/2006 > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mike.kirby <@t> nhls.ac.za Fri Mar 24 05:41:41 2006 From: mike.kirby <@t> nhls.ac.za (Mike Kirby) Date: Fri Mar 24 05:37:43 2006 Subject: [Histonet] Training med techs - some candid comments - PART II Message-ID: <452D0F6B16AA6E4092F6D3E9A9D97A620F8091@nhlsgpm002.NHLS.AC.ZA> Honourable Histologists. It is possible that I've misinterpreted Ms Orr's statements, and was overly sensitive, but for those who've been on the Net for some time will remember a similar fight about two years ago, when one of the subscribers brought up the same subject, and cast serious aspersions about us MT's and our alleged "inability" to be trained in Histopath techniques. I have no less respect for you than any other of the disciplines in the Med Tech world. I just get annoyed when one discipline bad mouths another (Inadvertently it would appear in Ms Orr's case). Lets call it a draw and leave it there. Oh, and Mr. Morken, for the record, I have cut frozen sections while the Path & surgeon were fulminating over my shoulder, and I might add, on one of those old fashioned sledge type microtomes that clamped on the bench or table and was attached to a cylinder of CO2 gas, which was used to freeze the specimen and chill the knife. It was an art, not a science, you had to ensure that the tissue was frozen enough to stick to the horizontal chuck, by blasting it with the gas, and that the knife was a few degrees warmer so you could cut a section. Try it sometime. Mr.M.Kirby Johannesburg South Africa. ********************************************************************************** The views expressed in this email are, unless otherwise stated, those of the author and not those of the National Health Laboratory Services or its management. The information in this e-mail is confidential and is intended solely for the addressee. Access to this e-mail by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken or omitted in reliance on this, is prohibited and may be unlawful. Whilst all reasonable steps are taken to ensure the accuracy and integrity of information and data transmitted electronically and to preserve the confidentiality thereof, no liability or responsibility whatsoever is accepted if information or data is, for whatever reason, corrupted or does not reach its intended destination. *********************************************************************************** From akbitting <@t> geisinger.edu Fri Mar 24 06:09:15 2006 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Mar 24 06:09:34 2006 Subject: [Histonet] CAP Guidelines for Microwaves Message-ID: Are any of the chemicals we heat in our microwaves toxic? I don't think most special stains solutions fall in that catagory. That's the first question to answer before getting to wrapped up in this ventilation issue? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 >>> "Joe Nocito" 3/23/2006 7:42 PM >>> but what about using a table top fume absorber with charcoal filters? CAP writes these questions so ambiguously that it becomes a joke. This is the main reason why I have a negative attitude towards CAP. Joe ----- Original Message ----- From: "Rene J Buesa" To: "Joe Nocito" ; "'Parker, Helayne'" ; Sent: Thursday, March 23, 2006 4:19 PM Subject: RE: [Histonet] CAP Guidelines for Microwaves > Joe: > It is not external ventilation to the MW oven what CAP wants, is a vent > connected from the microwave to an outlet and there is not a single > household MW oven with that characteristec so, for all practical purposes > the mensage keeps being: don't use them (the household appliance) for > histology work, specially if tissue processing is the objective of the > use. > Ren? J. > > Joe Nocito wrote: > I don't believe CAP is saying not to use them. They are saying use them > safely with ventilation and make sure they don't leak radiation. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J > Buesa > Sent: Thursday, March 23, 2006 11:35 AM > To: Parker, Helayne; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] CAP Guidelines for Microwaves > > Helayne: > In short, the message from CAP is: "do not use them!" > Unfortunate though! > Ren? J. > > "Parker, Helayne" wrote: > I was wondering if anyone new what CAP require of us if we have a common > household microwave that we use for special stains. > > Helayne Parker,HT (ASCP) > Histology Section Head > Skaggs Community Health Center > Branson, Missouri > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Blab-away for as little as 1?/min. Make PC-to-Phone Calls using Yahoo! > Messenger with Voice. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > --------------------------------- > Blab-away for as little as 1?/min. Make PC-to-Phone Calls using Yahoo! > Messenger with Voice. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.385 / Virus Database: 268.3.0/290 - Release Date: 3/23/2006 > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From jqb7 <@t> cdc.gov Fri Mar 24 06:16:34 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine) Date: Fri Mar 24 06:17:26 2006 Subject: [Histonet] CAP Guidelines for Microwaves Message-ID: Heating Bouin's is an issue, I would think. Jeanine Bartlett, BS, HT(ASCP) (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, March 24, 2006 7:09 AM To: histonet@lists.utsouthwestern.edu; jnocito@pathreflab.com; jnocito@satx.rr.com; HParker@Skaggs.Net; rjbuesa@yahoo.com Subject: Re: [Histonet] CAP Guidelines for Microwaves Are any of the chemicals we heat in our microwaves toxic? I don't think most special stains solutions fall in that catagory. That's the first question to answer before getting to wrapped up in this ventilation issue? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 >>> "Joe Nocito" 3/23/2006 7:42 PM >>> but what about using a table top fume absorber with charcoal filters? CAP writes these questions so ambiguously that it becomes a joke. This is the main reason why I have a negative attitude towards CAP. Joe ----- Original Message ----- From: "Rene J Buesa" To: "Joe Nocito" ; "'Parker, Helayne'" ; Sent: Thursday, March 23, 2006 4:19 PM Subject: RE: [Histonet] CAP Guidelines for Microwaves > Joe: > It is not external ventilation to the MW oven what CAP wants, is a vent > connected from the microwave to an outlet and there is not a single > household MW oven with that characteristec so, for all practical purposes > the mensage keeps being: don't use them (the household appliance) for > histology work, specially if tissue processing is the objective of the > use. > Ren? J. > > Joe Nocito wrote: > I don't believe CAP is saying not to use them. They are saying use them > safely with ventilation and make sure they don't leak radiation. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J > Buesa > Sent: Thursday, March 23, 2006 11:35 AM > To: Parker, Helayne; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] CAP Guidelines for Microwaves > > Helayne: > In short, the message from CAP is: "do not use them!" > Unfortunate though! > Ren? J. > > "Parker, Helayne" wrote: > I was wondering if anyone new what CAP require of us if we have a common > household microwave that we use for special stains. > > Helayne Parker,HT (ASCP) > Histology Section Head > Skaggs Community Health Center > Branson, Missouri > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Blab-away for as little as 1?/min. Make PC-to-Phone Calls using Yahoo! > Messenger with Voice. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > --------------------------------- > Blab-away for as little as 1?/min. Make PC-to-Phone Calls using Yahoo! > Messenger with Voice. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.385 / Virus Database: 268.3.0/290 - Release Date: 3/23/2006 > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmcardle <@t> ebsciences.com Fri Mar 24 07:22:10 2006 From: pmcardle <@t> ebsciences.com (Phil McArdle) Date: Fri Mar 24 07:22:21 2006 Subject: [Histonet] CAP Guidelines for Microwaves In-Reply-To: <2FABC6145388F24EBA8CBD6EC165BCCB02AFBA27@mail1-schc.skaggs.net> References: <2FABC6145388F24EBA8CBD6EC165BCCB02AFBA27@mail1-schc.skaggs.net> Message-ID: <4423F282.1090903@ebsciences.com> Hi: First of all, yes, I'm with a vendor who makes laboratory microwaves, so you can decide how much of an axe I have to grind, or even if you want to read this: Energy Beam Sciences, a pioneer in the use of microwaves in histology, has maintained for over a decade that consumer-grade microwaves have no place in a laboratory setting. Admittedly, over the years some users have managed to achieve varying levels of success in some "non-tissue-processing" applications like staining. However, I'd argue that a high number of failures due to this same approach has served to regress the art via anecdotal microwave "horror stories." Now that microwaves (both consumer-grade and true laboratory instrumentation) are in routine and widespread use in the histology laboratory, it should be unsurprising that CAP is taking this issue very seriously. CAP is not alone; microwaves in histology have been receiving increased interest from standards institutes such as CLSI (formerly NCCLS; see their publication GP-28A), and significantly, governmental agencies. Besides Europe's strict IVDD regulations, in the USA you have OSHA: see 29 CFR 1910.303(b)(2) which simply states that no food-grade microwave should be used for anything other than its intended purpose. From a workplace safety standpoint, the fact that consumer microwaves don't provide chamber fume extraction is extremely significant. Even seemingly benign reagents, when heated by any means, can easily produce high levels of vapor. Unimportant? Can't smell anything? Anyone who's suffered through O-Chem knows you can't go by smell or immediate symptoms, and it's commonly recognized that chronic exposure to pollutants is to be avoided. Consumer grade microwaves are prone to hot and cold spots within their chambers. In the proper context, this doesn't really matter; we've all had overcooked or undercooked meals (if we're unlucky, sometimes in the same meal!), but we live with it. This illustrates a lack of consistency or repeatability, the very factors that can prove problematic in a laboratory setting, for obvious reasons. In addition, generally speaking, consumer microwaves aren't designed or built to take the day-in-and-day-out demands of the histology laboratory, don't have calibrated output, and don't provide the means to measure output power, for example. These are not deficiencies, given the proper context: heating leftovers or reheating cold coffee at home... what's the worst that can happen? Now compare that with the worst that could happen with a biopsy. There's also the question of liability exposure. If your laboratory were party to any kind of lawsuit, whether as plaintiff or defendant, how comfortable would you be if it came out that a consumer appliance was being used, rather than appropriate laboratory instrumentation, even if it had nothing to do with the issue at hand? Now how does that $69 Wal-Mart microwave stack up? Late in 2005, after a series of conversations and e-mails with CAP representatives (and CLSI, whose publications CAP references), Energy Beam Sciences developed some recommendations to help with these new checklist items. An Acrobat file of said recommendations may be found on our website at http://www.ebsciences.com/pdf/EBS_CAP_RECOMMEND.pdf Also, feel free to browse our "library" section for articles, papers and protocols. Seriously, while I'd love to sell you one of our microwaves, I'm probably more interested in "advancing the art." And that means getting consumer microwaves out, and true laboratory microwaves in, even if they're not ours. End of rant and shameless commerce! Best regards, Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. Tel: 800.992.9037 x 341 Cell: 860.597.6796 Fax: 860.653.0422 PMcardle@ebsciences.com www.ebsciences.com "ADDING BRILLIANCE TO YOUR VISION" "I hate quotations. Tell me what you know." Ralph Waldo Emerson Parker, Helayne wrote: > I was wondering if anyone new what CAP require of us if we have a common > household microwave that we use for special stains. > > Helayne Parker,HT (ASCP) > Histology Section Head > Skaggs Community Health Center > Branson, Missouri > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. Tel: 800.992.9037 x 341 Cell: 860.597.6796 Fax: 860.653.0422 PMcardle@ebsciences.com www.ebsciences.com "ADDING BRILLIANCE TO YOUR VISION" "I hate quotations. Tell me what you know." Ralph Waldo Emerson From mariaforzan <@t> hotmail.com Fri Mar 24 07:46:25 2006 From: mariaforzan <@t> hotmail.com (maria forzan) Date: Fri Mar 24 07:46:36 2006 Subject: [Histonet] tryptase IHC Message-ID: Hello! We are having some trouble with our IHC stain for mast cell tryptase (Dako AA1 clone). We seem to get granular intracytoplasmic staining in several cells that are probably not mast cells but histiocytes or neoplastic plasma cells. I know that the antibody is very specific for mast cells and should not label anything else. So, does anybody have any ideas? Could it be the retrieval? Or the secondary? We use EnVision so it should not be a problem. Does anybody have any suggestions or has encounter a similar problem? Maria Forzan Finn Pathologists 44 (0)1379 854180 "Pebbles are inevitable" From kmerriam2003 <@t> yahoo.com Fri Mar 24 08:05:17 2006 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Fri Mar 24 08:05:22 2006 Subject: [Histonet] Santa Cruz anti mouse CD31 In-Reply-To: <20060323214028.8976.qmail@web31715.mail.mud.yahoo.com> Message-ID: <20060324140518.95935.qmail@web50315.mail.yahoo.com> I have used Santa Cruz's Rabbit anti-PECAM1 (CD31), but a different catalog #sc-28188. I use it at a 1:100 for FFPE mouse tissue; AR in steamer with Biocare Reveal. Kim GT Hebert wrote: Jackie, I have made a number of attempts, but without luck. The staining pattern just isn't what is should be (or better said, what it used to be). Santa Cruz sent me a free sample from there newest batch. I will keep Histonet posted if things change for the better. Gustave Gustave T. Hebert Scientist II Cardiovascular and Metabolic Diseases Wyeth Research Cambridge MA Jackie M O'Connor wrote: Has anyone had any luck with the newest lots of Santa Cruz PECAM-1 SC1506 in FFPE murine tissue? Jackie O' _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- New Yahoo! Messenger with Voice. Call regular phones from your PC for low, low rates. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Kim Merriam Novartis Cambridge, MA --------------------------------- Yahoo! Messenger with Voice. PC-to-Phone calls for ridiculously low rates. From hej01 <@t> health.state.ny.us Fri Mar 24 08:12:35 2006 From: hej01 <@t> health.state.ny.us (Helen E Johnson) Date: Fri Mar 24 08:12:47 2006 Subject: [Histonet] fibrin stain Message-ID: Hi Histonetters, Can someone recommend a good fibrin stain for mouse tissue and the protocol for it. Thanks. Helen (hej01@health.state.ny.us) From ploykasek <@t> phenopath.com Fri Mar 24 10:07:27 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri Mar 24 10:07:47 2006 Subject: [Histonet] tryptase IHC In-Reply-To: Message-ID: Maria, We are using a different clone - clone G3 from Chemicon. In our validation we did see some cross reactivity with basophils. It was positive on all cases of mast cell disease we tested & negative on other neoplasms & hematopoietic cell types. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Hello! > > We are having some trouble with our IHC stain for mast cell tryptase > (Dako AA1 clone). We seem to get granular intracytoplasmic staining > in several cells that are probably not mast cells but histiocytes or > neoplastic plasma cells. I know that the antibody is very specific > for mast cells and should not label anything else. So, does anybody > have any ideas? Could it be the retrieval? Or the secondary? We use > EnVision so it should not be a problem. > > Does anybody have any suggestions or has encounter a similar problem? > > Maria Forzan > > Finn Pathologists > > 44 (0)1379 854180 > > "Pebbles are inevitable" > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From MAUGER <@t> email.chop.edu Fri Mar 24 10:11:57 2006 From: MAUGER <@t> email.chop.edu (Joanne Mauger) Date: Fri Mar 24 10:12:38 2006 Subject: [Histonet] CD11b Message-ID: Hi All, Does anyone know of a CD11b antibody that works on FFPE? Thanks, Jo >>> Patti Loykasek 03/24/06 11:07 AM >>> Maria, We are using a different clone - clone G3 from Chemicon. In our validation we did see some cross reactivity with basophils. It was positive on all cases of mast cell disease we tested & negative on other neoplasms & hematopoietic cell types. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Hello! > > We are having some trouble with our IHC stain for mast cell tryptase > (Dako AA1 clone). We seem to get granular intracytoplasmic staining > in several cells that are probably not mast cells but histiocytes or > neoplastic plasma cells. I know that the antibody is very specific > for mast cells and should not label anything else. So, does anybody > have any ideas? Could it be the retrieval? Or the secondary? We use > EnVision so it should not be a problem. > > Does anybody have any suggestions or has encounter a similar problem? > > Maria Forzan > > Finn Pathologists > > 44 (0)1379 854180 > > "Pebbles are inevitable" > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LuckG <@t> empirehealth.org Fri Mar 24 10:25:32 2006 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Fri Mar 24 10:25:49 2006 Subject: [Histonet] CAP Guidelines for Microwaves Message-ID: Oh Why Not, I've taken the position (that from the CAP comment section of this question) that use of a non commercial grade microwave is still safe and usable provided you limit its use to heating of reagents such as PBS, agar, and tris or citrate buffers. Greg Luck Anatomic Pathology Supervisor Deaconess Med Center / EHS 800 W. 5th Ave Spokane, WA 99204 Phone 509.473.7394 Fax 509.473.7133 luckg@empirehealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Sent: Friday, March 24, 2006 4:17 AM To: Angela Bitting; histonet@lists.utsouthwestern.edu; jnocito@pathreflab.com; jnocito@satx.rr.com; HParker@Skaggs.Net; rjbuesa@yahoo.com Subject: RE: [Histonet] CAP Guidelines for Microwaves Heating Bouin's is an issue, I would think. Jeanine Bartlett, BS, HT(ASCP) (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, March 24, 2006 7:09 AM To: histonet@lists.utsouthwestern.edu; jnocito@pathreflab.com; jnocito@satx.rr.com; HParker@Skaggs.Net; rjbuesa@yahoo.com Subject: Re: [Histonet] CAP Guidelines for Microwaves Are any of the chemicals we heat in our microwaves toxic? I don't think most special stains solutions fall in that catagory. That's the first question to answer before getting to wrapped up in this ventilation issue? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 >>> "Joe Nocito" 3/23/2006 7:42 PM >>> but what about using a table top fume absorber with charcoal filters? CAP writes these questions so ambiguously that it becomes a joke. This is the main reason why I have a negative attitude towards CAP. Joe ----- Original Message ----- From: "Rene J Buesa" To: "Joe Nocito" ; "'Parker, Helayne'" ; Sent: Thursday, March 23, 2006 4:19 PM Subject: RE: [Histonet] CAP Guidelines for Microwaves > Joe: > It is not external ventilation to the MW oven what CAP wants, is a vent > connected from the microwave to an outlet and there is not a single > household MW oven with that characteristec so, for all practical purposes > the mensage keeps being: don't use them (the household appliance) for > histology work, specially if tissue processing is the objective of the > use. > Ren? J. > > Joe Nocito wrote: > I don't believe CAP is saying not to use them. They are saying use them > safely with ventilation and make sure they don't leak radiation. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J > Buesa > Sent: Thursday, March 23, 2006 11:35 AM > To: Parker, Helayne; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] CAP Guidelines for Microwaves > > Helayne: > In short, the message from CAP is: "do not use them!" > Unfortunate though! > Ren? J. > > "Parker, Helayne" wrote: > I was wondering if anyone new what CAP require of us if we have a common > household microwave that we use for special stains. > > Helayne Parker,HT (ASCP) > Histology Section Head > Skaggs Community Health Center > Branson, Missouri > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Blab-away for as little as 1?/min. Make PC-to-Phone Calls using Yahoo! > Messenger with Voice. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > --------------------------------- > Blab-away for as little as 1?/min. Make PC-to-Phone Calls using Yahoo! > Messenger with Voice. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.385 / Virus Database: 268.3.0/290 - Release Date: 3/23/2006 > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Mar 24 10:41:37 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 24 10:41:40 2006 Subject: [Histonet] CAP Guidelines for Microwaves In-Reply-To: <00e901c64f09$1497f200$e8bd0b43@yourxhtr8hvc4p> Message-ID: <20060324164137.24260.qmail@web61219.mail.yahoo.com> Joe: If you are using a MW oven that cannot vent out the internal air volume and you decide to do a "quick" fixation of a sample, the moment you open the door you, and your environment, will be contaminated with noxious HOT formalin fumes. It does not matter how much charcoal you place near the MW oven. Besides CAP, OSHA 29 CFR 1910.303(b)(2) states: "listed or labelled equipment shall be used or installed in accordance with any instrument included in the listing or labelling" in short meaning, you cannot use an instrument for other purpose different to what it was designed, and household MW oven were designed to heat and cook foods, not to process tissues. Besides that, all manufacturer now have a disclaimer stating that use of the MW oven for purposes other than they are intended to, void any guarantees. The ONLY solution would be to use the household MW oven INSIDE a fume hood. The new CAP Anatomic Pathology Checklist for its Accreditation Program ANP.29430 Microwave devices state that used OUTSIDE a fume hood should have an INTEGRAL fume extractor that needs to be properly connected to a house air handling system or approved stand alone laboratory air handling system. I still think that, at least for tissue processing, the message stands valid: "do not use them!" By the way, this has nothing to do with any "popularity contest" that for sure CAP will never win! Ren? J. Joe Nocito wrote: but what about using a table top fume absorber with charcoal filters? CAP writes these questions so ambiguously that it becomes a joke. This is the main reason why I have a negative attitude towards CAP. Joe ----- Original Message ----- From: "Rene J Buesa" To: "Joe Nocito" ; "'Parker, Helayne'" ; Sent: Thursday, March 23, 2006 4:19 PM Subject: RE: [Histonet] CAP Guidelines for Microwaves > Joe: > It is not external ventilation to the MW oven what CAP wants, is a vent > connected from the microwave to an outlet and there is not a single > household MW oven with that characteristec so, for all practical purposes > the mensage keeps being: don't use them (the household appliance) for > histology work, specially if tissue processing is the objective of the > use. > Ren? J. > > Joe Nocito wrote: > I don't believe CAP is saying not to use them. They are saying use them > safely with ventilation and make sure they don't leak radiation. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J > Buesa > Sent: Thursday, March 23, 2006 11:35 AM > To: Parker, Helayne; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] CAP Guidelines for Microwaves > > Helayne: > In short, the message from CAP is: "do not use them!" > Unfortunate though! > Ren? J. > > "Parker, Helayne" wrote: > I was wondering if anyone new what CAP require of us if we have a common > household microwave that we use for special stains. > > Helayne Parker,HT (ASCP) > Histology Section Head > Skaggs Community Health Center > Branson, Missouri > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Blab-away for as little as 1?/min. Make PC-to-Phone Calls using Yahoo! > Messenger with Voice. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > --------------------------------- > Blab-away for as little as 1?/min. Make PC-to-Phone Calls using Yahoo! > Messenger with Voice. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.385 / Virus Database: 268.3.0/290 - Release Date: 3/23/2006 > > --------------------------------- Yahoo! Messenger with Voice. PC-to-Phone calls for ridiculously low rates. From rjbuesa <@t> yahoo.com Fri Mar 24 10:54:33 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 24 10:54:37 2006 Subject: [Histonet] CAP Guidelines for Microwaves In-Reply-To: Message-ID: <20060324165433.73544.qmail@web61214.mail.yahoo.com> Greg; That is essentially my point. In the same way that you never would try to enter the Monaco race with a small FIAT 600 (but with a Porsche) you should never try to do things with a household MW oven that will require a lab designed one. Things I did (for years) with my 2 MW ovens: dry slides, all sorts of boosts to special stains, heating the citrate buffer before the HIER procedure and things of that sort. Never to process tissue (although I had to confess some "indiscretionts in that realm"). Regarding durability, my MW was used for more than 10 years continuously. Regarding reproducibility: it CAN be obtained as long as you calibrate it and know their capabilities [I even published a paper on that topic]. Now to the original question about CAP regulations and household MW ovens: the answre and approach from their point of view is the same: "do NOT used them" [for that purpose]. Ren? J. "Luck, Greg D." wrote: Oh Why Not, I've taken the position (that from the CAP comment section of this question) that use of a non commercial grade microwave is still safe and usable provided you limit its use to heating of reagents such as PBS, agar, and tris or citrate buffers. Greg Luck Anatomic Pathology Supervisor Deaconess Med Center / EHS 800 W. 5th Ave Spokane, WA 99204 Phone 509.473.7394 Fax 509.473.7133 luckg@empirehealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Sent: Friday, March 24, 2006 4:17 AM To: Angela Bitting; histonet@lists.utsouthwestern.edu; jnocito@pathreflab.com; jnocito@satx.rr.com; HParker@Skaggs.Net; rjbuesa@yahoo.com Subject: RE: [Histonet] CAP Guidelines for Microwaves Heating Bouin's is an issue, I would think. Jeanine Bartlett, BS, HT(ASCP) (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, March 24, 2006 7:09 AM To: histonet@lists.utsouthwestern.edu; jnocito@pathreflab.com; jnocito@satx.rr.com; HParker@Skaggs.Net; rjbuesa@yahoo.com Subject: Re: [Histonet] CAP Guidelines for Microwaves Are any of the chemicals we heat in our microwaves toxic? I don't think most special stains solutions fall in that catagory. That's the first question to answer before getting to wrapped up in this ventilation issue? Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 >>> "Joe Nocito" 3/23/2006 7:42 PM >>> but what about using a table top fume absorber with charcoal filters? CAP writes these questions so ambiguously that it becomes a joke. This is the main reason why I have a negative attitude towards CAP. Joe ----- Original Message ----- From: "Rene J Buesa" To: "Joe Nocito" ; "'Parker, Helayne'" ; Sent: Thursday, March 23, 2006 4:19 PM Subject: RE: [Histonet] CAP Guidelines for Microwaves > Joe: > It is not external ventilation to the MW oven what CAP wants, is a vent > connected from the microwave to an outlet and there is not a single > household MW oven with that characteristec so, for all practical purposes > the mensage keeps being: don't use them (the household appliance) for > histology work, specially if tissue processing is the objective of the > use. > Ren? J. > > Joe Nocito wrote: > I don't believe CAP is saying not to use them. They are saying use them > safely with ventilation and make sure they don't leak radiation. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J > Buesa > Sent: Thursday, March 23, 2006 11:35 AM > To: Parker, Helayne; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] CAP Guidelines for Microwaves > > Helayne: > In short, the message from CAP is: "do not use them!" > Unfortunate though! > Ren? J. > > "Parker, Helayne" wrote: > I was wondering if anyone new what CAP require of us if we have a common > household microwave that we use for special stains. > > Helayne Parker,HT (ASCP) > Histology Section Head > Skaggs Community Health Center > Branson, Missouri > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Blab-away for as little as 1?/min. Make PC-to-Phone Calls using Yahoo! > Messenger with Voice. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > --------------------------------- > Blab-away for as little as 1?/min. Make PC-to-Phone Calls using Yahoo! > Messenger with Voice. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.385 / Virus Database: 268.3.0/290 - Release Date: 3/23/2006 > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- New Yahoo! Messenger with Voice. Call regular phones from your PC and save big. From cpomajzl <@t> cpllabs.com Fri Mar 24 11:56:47 2006 From: cpomajzl <@t> cpllabs.com (Chris Pomajzl) Date: Fri Mar 24 11:54:26 2006 Subject: Fw: [Histonet] CAP Guidelines for Microwaves Message-ID: <005b01c64f6c$52643900$26fca8c0@CSP> Here is my $0.02 Ideally of course it would be better and wiser to have a laboratory designed industrial microwave oven in the histology lab. Now... does that mean that it is practical? Not necessarily. It is my impression that CAP merely wants laboratories to monitor and document the use and temperature reproducability of any and all MW ovens used in the lab. Seeing as how this is new wave of technology in the lab, I seriously doubt that anyone is suggesting that MW ovens not be used. They are too important to the functioning in today's laboratory. I think that CAP just wants to make sure that their use is strictly monitored and recorded. ----- Original Message ----- From: "Rene J Buesa" To: "Luck, Greg D." ; "'Bartlett, Jeanine'" ; "Angela Bitting" ; ; ; ; Sent: Friday, March 24, 2006 10:54 AM Subject: RE: [Histonet] CAP Guidelines for Microwaves > Greg; > That is essentially my point. In the same way that you never would try to enter the Monaco race with a small FIAT 600 (but with a Porsche) you should never try to do things with a household MW oven that will require a lab designed one. > Things I did (for years) with my 2 MW ovens: dry slides, all sorts of boosts to special stains, heating the citrate buffer before the HIER procedure and things of that sort. > Never to process tissue (although I had to confess some "indiscretionts in that realm"). > Regarding durability, my MW was used for more than 10 years continuously. > Regarding reproducibility: it CAN be obtained as long as you calibrate it and know their capabilities [I even published a paper on that topic]. > Now to the original question about CAP regulations and household MW ovens: the answre and approach from their point of view is the same: "do NOT used them" [for that purpose]. > Ren? J. > > "Luck, Greg D." wrote: > Oh Why Not, > > I've taken the position (that from the CAP comment section of this question) > that use of a non commercial grade microwave is still safe and usable > provided you limit its use to heating of reagents such as PBS, agar, and > tris or citrate buffers. > > Greg Luck > Anatomic Pathology Supervisor > Deaconess Med Center / EHS > 800 W. 5th Ave > Spokane, WA 99204 > Phone 509.473.7394 > Fax 509.473.7133 > luckg@empirehealth.org > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > Sent: Friday, March 24, 2006 4:17 AM > To: Angela Bitting; histonet@lists.utsouthwestern.edu; > jnocito@pathreflab.com; jnocito@satx.rr.com; HParker@Skaggs.Net; > rjbuesa@yahoo.com > Subject: RE: [Histonet] CAP Guidelines for Microwaves > > Heating Bouin's is an issue, I would think. > > > Jeanine Bartlett, BS, HT(ASCP) > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela > Bitting > Sent: Friday, March 24, 2006 7:09 AM > To: histonet@lists.utsouthwestern.edu; jnocito@pathreflab.com; > jnocito@satx.rr.com; HParker@Skaggs.Net; rjbuesa@yahoo.com > Subject: Re: [Histonet] CAP Guidelines for Microwaves > > Are any of the chemicals we heat in our microwaves toxic? I don't think most > special stains solutions fall in that catagory. That's the first question to > answer before getting to wrapped up in this ventilation issue? > > Angela Bitting, HT(ASCP) > Technical Specialist, Histology > Geisinger Medical Center > 100 N Academy Ave. MC 23-00 > Danville, PA 17822 > phone 570-214-9634 > fax 570-271-5916 > > >>> "Joe Nocito" 3/23/2006 7:42 PM >>> > > but what about using a table top fume absorber with charcoal filters? CAP > writes these questions so ambiguously that it becomes a joke. This is the > main reason why I have a negative attitude towards CAP. > > Joe > ----- Original Message ----- > From: "Rene J Buesa" > To: "Joe Nocito" ; "'Parker, Helayne'" > ; > Sent: Thursday, March 23, 2006 4:19 PM > Subject: RE: [Histonet] CAP Guidelines for Microwaves > > > > Joe: > > It is not external ventilation to the MW oven what CAP wants, is a vent > > connected from the microwave to an outlet and there is not a single > > household MW oven with that characteristec so, for all practical purposes > > the mensage keeps being: don't use them (the household appliance) for > > histology work, specially if tissue processing is the objective of the > > use. > > Ren? J. > > > > Joe Nocito wrote: > > I don't believe CAP is saying not to use them. They are saying use them > > safely with ventilation and make sure they don't leak radiation. > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J > > Buesa > > Sent: Thursday, March 23, 2006 11:35 AM > > To: Parker, Helayne; histonet@lists.utsouthwestern.edu > > Subject: Re: [Histonet] CAP Guidelines for Microwaves > > > > Helayne: > > In short, the message from CAP is: "do not use them!" > > Unfortunate though! > > Ren? J. > > > > "Parker, Helayne" wrote: > > I was wondering if anyone new what CAP require of us if we have a common > > household microwave that we use for special stains. > > > > Helayne Parker,HT (ASCP) > > Histology Section Head > > Skaggs Community Health Center > > Branson, Missouri > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > > --------------------------------- > > Blab-away for as little as 1?/min. Make PC-to-Phone Calls using Yahoo! > > Messenger with Voice. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > > > > > > --------------------------------- > > Blab-away for as little as 1?/min. Make PC-to-Phone Calls using Yahoo! > > Messenger with Voice. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > > No virus found in this incoming message. > > Checked by AVG Free Edition. > > Version: 7.1.385 / Virus Database: 268.3.0/290 - Release Date: 3/23/2006 > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > IMPORTANT WARNING: The information in this message (and the documents > attached to it, if any) is confidential and may be legally privileged. It is > intended solely for the addressee. Access to this message by anyone else is > unauthorized. If you are not the intended recipient, any disclosure, > copying, distribution or any action taken, or omitted to be taken, in > reliance on it is prohibited and may be unlawful. If you have received this > message in error, please delete all electronic copies of this message (and > the documents attached to it, if any), destroy any hard copies you may have > created and notify me immediately by replying to this email. Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > New Yahoo! Messenger with Voice. Call regular phones from your PC and save big. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From leslie.duncan <@t> bms.com Fri Mar 24 12:28:22 2006 From: leslie.duncan <@t> bms.com (Leslie D Duncan) Date: Fri Mar 24 12:31:26 2006 Subject: [Histonet] Staining Glycol Methacrylate Message-ID: <44243A46.3050508@bms.com> Hi, I am experimenting with glycol methacrylate embedded rodent testes. I am looking for a good H&E staining protocol to use that will give results comparable to paraffin-embedded tissue (per request of my management). We have dehydrated on the tissue processor to 100% ethanol, ilfiltrated and embedded with JB-4, and sectioned at 1-2 microns. The sections are placed on Poly L-lysine slides. Any protocols, ideas, and/or suggestions will be greatly appreciated! Thank you, Leslie Duncan Bristol-Myers Squibb, PRI From Tracey.Lenek <@t> CLS.ab.ca Fri Mar 24 12:35:08 2006 From: Tracey.Lenek <@t> CLS.ab.ca (Tracey.Lenek@CLS.ab.ca) Date: Fri Mar 24 12:35:14 2006 Subject: [Histonet] Ventana IVIEW Detection Kits Message-ID: <4BC300747AF87A48BCDF8E48BC2885CE02C1D598@mail1.calgary.com> Just a general question for all the Ventana automated IHC equipment users - this time last year we found that the IVIEW detection kits were overstaining. Now we are experiencing weak staining. Has anyone else found variabilities with the staining in their kits? We have relayed our issues to Ventana but as usual are the only ones complaining. Tracey CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify the sender immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. From sjchtascp <@t> yahoo.com Fri Mar 24 12:08:33 2006 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Fri Mar 24 13:08:38 2006 Subject: [Histonet] endothelial marker FFPE Rat Message-ID: <20060324180833.66058.qmail@web38202.mail.mud.yahoo.com> Has anyone any knowledge of any endothelial markers, such as CD31, RECA, ect.... for FFPE for a rat muscle angiogenesis study. Steve --------------------------------- Blab-away for as little as 1?/min. Make PC-to-Phone Calls using Yahoo! Messenger with Voice. From gpbnas <@t> yahoo.es Fri Mar 24 13:12:57 2006 From: gpbnas <@t> yahoo.es (Guillermo Palao) Date: Fri Mar 24 13:13:05 2006 Subject: [Histonet] RE: anti-human TNFR-1 anti-mouse TNFR-1 and 2 In-Reply-To: <4422D22D.5070507@chiron.com> Message-ID: <20060324191258.74730.qmail@web26210.mail.ukl.yahoo.com> Again, any suggestions for good commercially available antibody for mouse TNRs 1 and 2 (CD120 a,b) are most welcome. Thanks for all previous and future help in saving time with painful optimizations of non-working antibodies. Guillermo Alessandro Serra escribi?: Dear All Has anybody used succesfully any commercially available anti-human TNFR-1 (CD120a)? If yes, could you please be so kind to tell me which one? Thank you very much for your help Alessandro Serra, BSc. --------------------------------- LLama Gratis a cualquier PC del Mundo. Llamadas a fijos y m?viles desde 1 c?ntimo por minuto. http://es.voice.yahoo.com From liz <@t> premierlab.com Fri Mar 24 13:31:52 2006 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Mar 24 13:32:07 2006 Subject: [Histonet] endothelial marker FFPE Rat In-Reply-To: <20060324180833.66058.qmail@web38202.mail.mud.yahoo.com> Message-ID: <000a01c64f79$9a72f4e0$0300a8c0@Chlipala> Steve I have used the goat-anti mouse PECAM-1 from santa cruz for rat tissue and it works. The only thing is that I happen to have the older lot number that works in FFPE tissues. I don't know what newer lots will do. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Coakley Sent: Friday, March 24, 2006 11:09 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] endothelial marker FFPE Rat Has anyone any knowledge of any endothelial markers, such as CD31, RECA, ect.... for FFPE for a rat muscle angiogenesis study. Steve --------------------------------- Blab-away for as little as 1?/min. Make PC-to-Phone Calls using Yahoo! Messenger with Voice. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From la.sebree <@t> hosp.wisc.edu Fri Mar 24 13:36:22 2006 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Fri Mar 24 13:36:27 2006 Subject: [Histonet] Ventana IVIEW Detection Kits Message-ID: We have not experienced that phenomenon ever that I can recall. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tracey.Lenek@CLS.ab.ca Sent: Friday, March 24, 2006 12:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana IVIEW Detection Kits Just a general question for all the Ventana automated IHC equipment users - this time last year we found that the IVIEW detection kits were overstaining. Now we are experiencing weak staining. Has anyone else found variabilities with the staining in their kits? We have relayed our issues to Ventana but as usual are the only ones complaining. Tracey CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify the sender immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Fri Mar 24 13:40:43 2006 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Mar 24 13:40:52 2006 Subject: [Histonet] peripheral axons in soft tissue Message-ID: <000001c64f7a$d6a91f10$0300a8c0@Chlipala> Hello Everyone Is there a stain that I can use that will detect peripheral nerve (axons) in soft tissue. I have checked around a bit and it looks like performing a Bodian would work. I would appreciate any input or advice on this. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 From dusko.trajkovic <@t> pfizer.com Fri Mar 24 13:46:35 2006 From: dusko.trajkovic <@t> pfizer.com (Trajkovic, Dusko) Date: Fri Mar 24 13:46:48 2006 Subject: [Histonet] endothelial marker FFPE Rat Message-ID: <3AD0BD3142459B4E9B12CBEAFF2B89B202A94F4F@lajamrexm01.amer.pfizer.com> I had Santa Cruz goat antibody PECAM-1 sc-1505 (lot# G1103) that worked well on rat tissue, however the new lot number (C2405) which I received last week, is not working well at all. I will be getting a new lot number from them next Tuesday. I will post my results later in the week. Dusko Trajkovic -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Friday, March 24, 2006 11:32 AM To: 'Steven Coakley'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] endothelial marker FFPE Rat Steve I have used the goat-anti mouse PECAM-1 from santa cruz for rat tissue and it works. The only thing is that I happen to have the older lot number that works in FFPE tissues. I don't know what newer lots will do. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Coakley Sent: Friday, March 24, 2006 11:09 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] endothelial marker FFPE Rat Has anyone any knowledge of any endothelial markers, such as CD31, RECA, ect.... for FFPE for a rat muscle angiogenesis study. Steve --------------------------------- Blab-away for as little as 1?/min. Make PC-to-Phone Calls using Yahoo! Messenger with Voice. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately. From MMcCOY <@t> lakelandregional.org Fri Mar 24 14:18:53 2006 From: MMcCOY <@t> lakelandregional.org (Mary McCoy) Date: Fri Mar 24 14:19:20 2006 Subject: [Histonet] Job openings in Michigan Message-ID: Lakeland Regional Health System Position: Histotechnologist Salary: Competitive Place of work: Lakeland Hospital, St. Joseph, MI Position status: Two, full-time permanent positions Number of Vacant Positions: 02 Description of position: Performs technical functions necessary in preparation of tissues for microscopic examination by the pathologist; assists the coordinator with quality assurance monitoring; management of the immunohistochemical (IHC) stains and associated quality control; assists the coordinator with the implementation of new procedures and the evaluation of new instruments; and assists the coordinator with training as necessary. HT or HTL (ASCP) certification is preferred; we will consider a Bachelors degree in a related field and training in the department. Lakeland offers a comprehensive benefits package, competitive salaries, PTO, educational reimbursement, and much more. Lakeland is located on the shores of beautiful Lake Michigan and is just 90 minutes from Chicago. Please send cover letter and resume to Marianna Cerecke, Lakeland Regional Health System, 6418 Deans Hill Rd, Berrien Center, MI 49102. Or fax to: 269-473-3069. For questions call 269-473-3069 or visit our website at www.lakelandhealth.org to apply on-line. From asmith <@t> mail.barry.edu Fri Mar 24 15:08:38 2006 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Mar 24 15:08:44 2006 Subject: [Histonet] peripheral axons in soft tissue Message-ID: <5D2189E74151CC42BEC02906BA8996322B91AD@exchsrv01.barrynet.barry.edu> In my hands, the most reliable technique for demonstrating peripheral nerves is Kiernan's physical developer method. [pp. 371-372 of the 3rd edition of J.A. Kiernan's HISTOLOGICAL AND HISTOCHEMICAL METHODS.] Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Friday, March 24, 2006 2:41 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] peripheral axons in soft tissue Hello Everyone Is there a stain that I can use that will detect peripheral nerve (axons) in soft tissue. I have checked around a bit and it looks like performing a Bodian would work. I would appreciate any input or advice on this. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From jstn192 <@t> yahoo.com Fri Mar 24 15:37:00 2006 From: jstn192 <@t> yahoo.com (Justin Thomas) Date: Fri Mar 24 15:37:03 2006 Subject: [Histonet] RE: Input on Milestone RHS-1 and RHS-2 Message-ID: <20060324213700.4795.qmail@web35704.mail.mud.yahoo.com> is anyone familiar with the milestone RHS-1 and RHS-2 microwaves? If so, what are your pros and cons for the two units. Thanks in advance for any input! -thomas --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. From mgdelaware <@t> comcast.net Fri Mar 24 16:27:07 2006 From: mgdelaware <@t> comcast.net (Marian powers) Date: Fri Mar 24 16:26:17 2006 Subject: [Histonet] Autodial alarms Message-ID: <000801c64f92$163b1c70$be4dff45@MEGAN> Looking for an autodial alarm for a tissue processor, any recommendations? Thanks in advance. Marian From kccatunda <@t> terra.com.br Fri Mar 24 18:08:12 2006 From: kccatunda <@t> terra.com.br (Katia Cristina Catunda) Date: Fri Mar 24 18:08:25 2006 Subject: [Histonet] Hybrid capture, CISH References: <2533F91DF0FC3F48AE4919B3197765ED2E9619@GUR-PR-EXA1.Ranbaxy.com> Message-ID: <004401c64fa0$35052890$17d0fea9@privatexx> Dear collegues, I would like some information about vendors for Hybrid Capture equipments and supplies as for CISH, can anybody help me (just know Digene and Zymer respectively). And how about some tips for Hybridizators? We've found from boekel and zymed with a great price diferenciation. Thanks Katia From jeannie_heck <@t> yahoo.com Sat Mar 25 09:16:17 2006 From: jeannie_heck <@t> yahoo.com (Jeannie Heck) Date: Sat Mar 25 09:16:25 2006 Subject: [Histonet] Xylene exposure Message-ID: <20060325151617.49980.qmail@web30705.mail.mud.yahoo.com> What special precautions, if any, does your facility make for pregnant employees whose job duties involve the use of, or exposure to xylene? __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From rjbuesa <@t> yahoo.com Sat Mar 25 11:29:41 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Mar 25 11:29:48 2006 Subject: [Histonet] Xylene exposure In-Reply-To: <20060325151617.49980.qmail@web30705.mail.mud.yahoo.com> Message-ID: <20060325172941.74110.qmail@web61213.mail.yahoo.com> Three in my staff confronted that situation (not simultaneously!!) and I tried to rotate each to tasks with much less exposure. On the other hand our monitoring system always came as "0" (13 ppm / hour) which is approximately 9% for xylene STEL and 13% for xylene TWA exposure limits. I always found that reasignment of tasks is the best solution but it requires a strongly implemented crosstraining program. Hope this will help! Ren? J. Jeannie Heck wrote: What special precautions, if any, does your facility make for pregnant employees whose job duties involve the use of, or exposure to xylene? __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- New Yahoo! Messenger with Voice. Call regular phones from your PC and save big. From gu.lang <@t> gmx.at Sat Mar 25 12:41:28 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sat Mar 25 12:41:27 2006 Subject: AW: [Histonet] Xylene exposure In-Reply-To: <20060325151617.49980.qmail@web30705.mail.mud.yahoo.com> Message-ID: <000001c6503b$ba791db0$eeeea8c0@SERVER01> We are lucky to have xylen-free rooms, where pregnant colleagues can cut and do administrative duties. In general they are not allowed to get in touch with xylen, formalin and fresh material. Gudrun Lang Austria -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Jeannie Heck Gesendet: Samstag, 25. M?rz 2006 16:16 An: Histonet e-mail ?'s Betreff: [Histonet] Xylene exposure What special precautions, if any, does your facility make for pregnant employees whose job duties involve the use of, or exposure to xylene? __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Sat Mar 25 12:47:20 2006 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Sat Mar 25 12:48:01 2006 Subject: [Histonet] RE:H&E on GMA sections Message-ID: Well.....after leaving my slide-mounted GMA-embedded sections in a 60C oven for an hour. I'll rinse them in tapwater and then leave them in Hx for about 20mins. After that, I'll rinse them in tapwater, have a look down the m'scope to note the intensity of Hx staining and differentiate in 0.5% HCL in 70% Alc ( water if you want)until I think that the balance is good. Then I will leave in 0.5% Eosin for as long as I want ( say, 20mins). Then wash in tapwater until I achieve the best Hx-Eosin balance. I then blot dry my resin sections and place them in the same oven to dry...min an hour. Then put them in xylene and , after 15-odd mins ..mount in DPX! No times are critical, IMHO. That's the great thing about H&E....just get a routine that is reproducable/consistent for your lab. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 28, Issue 41 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Staining Glycol Methacrylate (Leslie D Duncan) 2. Ventana IVIEW Detection Kits (Tracey.Lenek@CLS.ab.ca) 3. endothelial marker FFPE Rat (Steven Coakley) 4. RE: anti-human TNFR-1 anti-mouse TNFR-1 and 2 (Guillermo Palao) 5. RE: endothelial marker FFPE Rat (Elizabeth Chlipala) 6. RE: Ventana IVIEW Detection Kits (Sebree Linda A.) 7. peripheral axons in soft tissue (Elizabeth Chlipala) 8. RE: endothelial marker FFPE Rat (Trajkovic, Dusko) 9. Job openings in Michigan (Mary McCoy) 10. RE: peripheral axons in soft tissue (Smith, Allen) 11. RE: Input on Milestone RHS-1 and RHS-2 (Justin Thomas) 12. Autodial alarms (Marian powers) 13. Hybrid capture, CISH (Katia Cristina Catunda) 14. Xylene exposure (Jeannie Heck) 15. Re: Xylene exposure (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Fri, 24 Mar 2006 12:28:22 -0600 From: Leslie D Duncan Subject: [Histonet] Staining Glycol Methacrylate To: histonet@lists.utsouthwestern.edu Message-ID: <44243A46.3050508@bms.com> Content-Type: text/plain; charset="iso-8859-1" Hi, I am experimenting with glycol methacrylate embedded rodent testes. I am looking for a good H&E staining protocol to use that will give results comparable to paraffin-embedded tissue (per request of my management). We have dehydrated on the tissue processor to 100% ethanol, ilfiltrated and embedded with JB-4, and sectioned at 1-2 microns. The sections are placed on Poly L-lysine slides. Any protocols, ideas, and/or suggestions will be greatly appreciated! Thank you, Leslie Duncan Bristol-Myers Squibb, PRI ------------------------------ Message: 2 Date: Fri, 24 Mar 2006 11:35:08 -0700 From: Subject: [Histonet] Ventana IVIEW Detection Kits To: Message-ID: <4BC300747AF87A48BCDF8E48BC2885CE02C1D598@mail1.calgary.com> Content-Type: text/plain; charset="iso-8859-1" Just a general question for all the Ventana automated IHC equipment users - this time last year we found that the IVIEW detection kits were overstaining. Now we are experiencing weak staining. Has anyone else found variabilities with the staining in their kits? We have relayed our issues to Ventana but as usual are the only ones complaining. Tracey CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify the sender immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. ------------------------------ Message: 3 Date: Fri, 24 Mar 2006 10:08:33 -0800 (PST) From: Steven Coakley Subject: [Histonet] endothelial marker FFPE Rat To: Histonet@lists.utsouthwestern.edu Message-ID: <20060324180833.66058.qmail@web38202.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Has anyone any knowledge of any endothelial markers, such as CD31, RECA, ect.... for FFPE for a rat muscle angiogenesis study. Steve --------------------------------- Blab-away for as little as 1"/min. Make PC-to-Phone Calls using Yahoo! Messenger with Voice. ------------------------------ Message: 4 Date: Fri, 24 Mar 2006 20:12:57 +0100 (CET) From: Guillermo Palao Subject: [Histonet] RE: anti-human TNFR-1 anti-mouse TNFR-1 and 2 To: Histonet Message-ID: <20060324191258.74730.qmail@web26210.mail.ukl.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Again, any suggestions for good commercially available antibody for mouse TNRs 1 and 2 (CD120 a,b) are most welcome. Thanks for all previous and future help in saving time with painful optimizations of non-working antibodies. Guillermo Alessandro Serra escribis: Dear All Has anybody used succesfully any commercially available anti-human TNFR-1 (CD120a)? If yes, could you please be so kind to tell me which one? Thank you very much for your help Alessandro Serra, BSc. --------------------------------- LLama Gratis a cualquier PC del Mundo. Llamadas a fijos y msviles desde 1 cintimo por minuto. http://es.voice.yahoo.com ------------------------------ Message: 5 Date: Fri, 24 Mar 2006 12:31:52 -0700 From: "Elizabeth Chlipala" Subject: RE: [Histonet] endothelial marker FFPE Rat To: "'Steven Coakley'" , Message-ID: <000a01c64f79$9a72f4e0$0300a8c0@Chlipala> Content-Type: text/plain; charset="iso-8859-1" Steve I have used the goat-anti mouse PECAM-1 from santa cruz for rat tissue and it works. The only thing is that I happen to have the older lot number that works in FFPE tissues. I don't know what newer lots will do. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Coakley Sent: Friday, March 24, 2006 11:09 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] endothelial marker FFPE Rat Has anyone any knowledge of any endothelial markers, such as CD31, RECA, ect.... for FFPE for a rat muscle angiogenesis study. Steve --------------------------------- Blab-away for as little as 1"/min. Make PC-to-Phone Calls using Yahoo! Messenger with Voice. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 24 Mar 2006 13:36:22 -0600 From: "Sebree Linda A." Subject: RE: [Histonet] Ventana IVIEW Detection Kits To: , Message-ID: Content-Type: text/plain; charset="US-ASCII" We have not experienced that phenomenon ever that I can recall. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tracey.Lenek@CLS.ab.ca Sent: Friday, March 24, 2006 12:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana IVIEW Detection Kits Just a general question for all the Ventana automated IHC equipment users - this time last year we found that the IVIEW detection kits were overstaining. Now we are experiencing weak staining. Has anyone else found variabilities with the staining in their kits? We have relayed our issues to Ventana but as usual are the only ones complaining. Tracey CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify the sender immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Fri, 24 Mar 2006 12:40:43 -0700 From: "Elizabeth Chlipala" Subject: [Histonet] peripheral axons in soft tissue To: Message-ID: <000001c64f7a$d6a91f10$0300a8c0@Chlipala> Content-Type: text/plain; charset="us-ascii" Hello Everyone Is there a stain that I can use that will detect peripheral nerve (axons) in soft tissue. I have checked around a bit and it looks like performing a Bodian would work. I would appreciate any input or advice on this. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 ------------------------------ Message: 8 Date: Fri, 24 Mar 2006 11:46:35 -0800 From: "Trajkovic, Dusko" Subject: RE: [Histonet] endothelial marker FFPE Rat To: "Steven Coakley" , Message-ID: <3AD0BD3142459B4E9B12CBEAFF2B89B202A94F4F@lajamrexm01.amer.pfizer.com> Content-Type: text/plain; charset="iso-8859-1" I had Santa Cruz goat antibody PECAM-1 sc-1505 (lot# G1103) that worked well on rat tissue, however the new lot number (C2405) which I received last week, is not working well at all. I will be getting a new lot number from them next Tuesday. I will post my results later in the week. Dusko Trajkovic -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Friday, March 24, 2006 11:32 AM To: 'Steven Coakley'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] endothelial marker FFPE Rat Steve I have used the goat-anti mouse PECAM-1 from santa cruz for rat tissue and it works. The only thing is that I happen to have the older lot number that works in FFPE tissues. I don't know what newer lots will do. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Coakley Sent: Friday, March 24, 2006 11:09 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] endothelial marker FFPE Rat Has anyone any knowledge of any endothelial markers, such as CD31, RECA, ect.... for FFPE for a rat muscle angiogenesis study. Steve --------------------------------- Blab-away for as little as 1"/min. Make PC-to-Phone Calls using Yahoo! Messenger with Voice. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately. ------------------------------ Message: 9 Date: Fri, 24 Mar 2006 15:18:53 -0500 From: "Mary McCoy" Subject: [Histonet] Job openings in Michigan To: histonet@lists.utsouthwestern.edu Cc: Marianna Cerecke Message-ID: Content-Type: text/plain; charset=us-ascii Lakeland Regional Health System Position: Histotechnologist Salary: Competitive Place of work: Lakeland Hospital, St. Joseph, MI Position status: Two, full-time permanent positions Number of Vacant Positions: 02 Description of position: Performs technical functions necessary in preparation of tissues for microscopic examination by the pathologist; assists the coordinator with quality assurance monitoring; management of the immunohistochemical (IHC) stains and associated quality control; assists the coordinator with the implementation of new procedures and the evaluation of new instruments; and assists the coordinator with training as necessary. HT or HTL (ASCP) certification is preferred; we will consider a Bachelors degree in a related field and training in the department. Lakeland offers a comprehensive benefits package, competitive salaries, PTO, educational reimbursement, and much more. Lakeland is located on the shores of beautiful Lake Michigan and is just 90 minutes from Chicago. Please send cover letter and resume to Marianna Cerecke, Lakeland Regional Health System, 6418 Deans Hill Rd, Berrien Center, MI 49102. Or fax to: 269-473-3069. For questions call 269-473-3069 or visit our website at www.lakelandhealth.org to apply on-line. ------------------------------ Message: 10 Date: Fri, 24 Mar 2006 16:08:38 -0500 From: "Smith, Allen" Subject: RE: [Histonet] peripheral axons in soft tissue To: "Elizabeth Chlipala" Cc: histonet@lists.utsouthwestern.edu Message-ID: <5D2189E74151CC42BEC02906BA8996322B91AD@exchsrv01.barrynet.barry.edu> Content-Type: text/plain; charset="us-ascii" In my hands, the most reliable technique for demonstrating peripheral nerves is Kiernan's physical developer method. [pp. 371-372 of the 3rd edition of J.A. Kiernan's HISTOLOGICAL AND HISTOCHEMICAL METHODS.] Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Friday, March 24, 2006 2:41 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] peripheral axons in soft tissue Hello Everyone Is there a stain that I can use that will detect peripheral nerve (axons) in soft tissue. I have checked around a bit and it looks like performing a Bodian would work. I would appreciate any input or advice on this. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) ------------------------------ Message: 11 Date: Fri, 24 Mar 2006 13:37:00 -0800 (PST) From: Justin Thomas Subject: [Histonet] RE: Input on Milestone RHS-1 and RHS-2 To: histonet@lists.utsouthwestern.edu Message-ID: <20060324213700.4795.qmail@web35704.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 is anyone familiar with the milestone RHS-1 and RHS-2 microwaves? If so, what are your pros and cons for the two units. Thanks in advance for any input! -thomas --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2"/min or less. ------------------------------ Message: 12 Date: Fri, 24 Mar 2006 17:27:07 -0500 From: "Marian powers" Subject: [Histonet] Autodial alarms To: Message-ID: <000801c64f92$163b1c70$be4dff45@MEGAN> Content-Type: text/plain; charset="iso-8859-1" Looking for an autodial alarm for a tissue processor, any recommendations? Thanks in advance. Marian ------------------------------ Message: 13 Date: Fri, 24 Mar 2006 21:08:12 -0300 From: "Katia Cristina Catunda" Subject: [Histonet] Hybrid capture, CISH To: Message-ID: <004401c64fa0$35052890$17d0fea9@privatexx> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Dear collegues, I would like some information about vendors for Hybrid Capture equipments and supplies as for CISH, can anybody help me (just know Digene and Zymer respectively). And how about some tips for Hybridizators? We've found from boekel and zymed with a great price diferenciation. Thanks Katia ------------------------------ Message: 14 Date: Sat, 25 Mar 2006 07:16:17 -0800 (PST) From: Jeannie Heck Subject: [Histonet] Xylene exposure To: Histonet e-mail ?'s Message-ID: <20060325151617.49980.qmail@web30705.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 What special precautions, if any, does your facility make for pregnant employees whose job duties involve the use of, or exposure to xylene? __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com ------------------------------ Message: 15 Date: Sat, 25 Mar 2006 09:29:41 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Xylene exposure To: Jeannie Heck , Histonet e-mail ?'s Message-ID: <20060325172941.74110.qmail@web61213.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Three in my staff confronted that situation (not simultaneously!!) and I tried to rotate each to tasks with much less exposure. On the other hand our monitoring system always came as "0" (13 ppm / hour) which is approximately 9% for xylene STEL and 13% for xylene TWA exposure limits. I always found that reasignment of tasks is the best solution but it requires a strongly implemented crosstraining program. Hope this will help! Reni J. Jeannie Heck wrote: What special precautions, if any, does your facility make for pregnant employees whose job duties involve the use of, or exposure to xylene? __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- New Yahoo! Messenger with Voice. Call regular phones from your PC and save big. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 28, Issue 41 **************************************** -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.385 / Virus Database: 268.2.6/287 - Release Date: 21/03/2006 From RCHIOVETTI <@t> aol.com Sat Mar 25 20:21:20 2006 From: RCHIOVETTI <@t> aol.com (RCHIOVETTI@aol.com) Date: Sat Mar 25 20:21:30 2006 Subject: [Histonet] colored OC T media - not TBS Message-ID: <24a.947e488.315754a0@aol.com> In a message dated 3/23/2006 4:32:05 PM US Mountain Standard Time, mbmphoto@gmail.com writes: > Does anyone know where I can buy "colored" OCT freezing media that is > NOT TBS media? > > Many thanks. > > Maria Bartola Mejia > > Maria, Try Richard-Allan Scientific. Go to: http://www.rallansci.com Click on the link to "histology," then "cryotomy." There are five colors to choose from. Perhaps this is what you're looking for? Cheers, Bob Robert (Bob) Chiovetti, Ph.D. The Microscope Works Arizona's Microscopy Resource 132 North Elster Drive Tucson, AZ 85710-3212 USA Tel./Fax 520-546-4986 Manufacturers' Representatives Member, Arizona Small Business Association - ASBA From RCHIOVETTI <@t> aol.com Sat Mar 25 20:35:36 2006 From: RCHIOVETTI <@t> aol.com (RCHIOVETTI@aol.com) Date: Sat Mar 25 20:35:45 2006 Subject: [Histonet] Autodial alarms Message-ID: <30b.10a2cf6.315757f8@aol.com> In a message dated 3/24/2006 3:26:45 PM US Mountain Standard Time, mgdelaware@comcast.net writes: > Looking for an autodial alarm for a tissue processor, any recommendations? > Thanks in advance. > > Marian > Marian, We used to buy autodialers from Radio Shack a few years ago when they had a hard-wired model (connected the two leads from the processor to the back of the dialer). The current models are now wireless; I haven't tried one of the new models, but they should work fine. Go to your local Radio Shack and look at the automatic dialers in their home security section. I think it's called "Plug 'n Power Wireless Dialer," or something like that. I think the new ones handle contacts that are both types...normally open and normally closed. Check the processor's technical specs, and the staff can probably advise you at the Radio Shack store. Cheers, Bob Robert (Bob) Chiovetti, Ph.D. The Microscope Works Arizona's Microscopy Resource 132 North Elster Drive Tucson, AZ 85710-3212 USA Tel./Fax 520-546-4986 Member, Arizona Small Business Association - ASBA From jnocito <@t> satx.rr.com Sun Mar 26 06:05:37 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sun Mar 26 06:05:43 2006 Subject: [Histonet] CAP Guidelines for Microwaves References: <20060324164137.24260.qmail@web61219.mail.yahoo.com> Message-ID: <002201c650cd$986a5120$e8bd0b43@yourxhtr8hvc4p> Okay, I did put my microwave in a fume hood. I don't process tissue in the microwave. I did have a "laboratory" grade tissue processing microwave processor that fried my specimens because the magnetron went bad. I have been using household microwaves for special staining and immunos. Hey, I remember when Biogenex first came out with microwave antigen retrieval using a lead based liquid and I don't have an residual effects, residual effects, residual eeefects. As many of you know, we will all see what happens during onsite CAP inspections. Speaking of CAP inspections, remember, starting in April, CAP will start their so called "non- announced" inspections. Have a happy weekend all. I'm still trying to unpack the lab by tomorrow, because we have to be fully functional by Monday. What day is this? Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Rene J Buesa" To: "Joe Nocito" ; "Joe Nocito" ; "'Parker, Helayne'" ; Sent: Friday, March 24, 2006 10:41 AM Subject: Re: [Histonet] CAP Guidelines for Microwaves > Joe: > If you are using a MW oven that cannot vent out the internal air volume > and you decide to do a "quick" fixation of a sample, the moment you open > the door you, and your environment, will be contaminated with noxious HOT > formalin fumes. It does not matter how much charcoal you place near the MW > oven. > Besides CAP, OSHA 29 CFR 1910.303(b)(2) states: "listed or labelled > equipment shall be used or installed in accordance with any instrument > included in the listing or labelling" in short meaning, you cannot use an > instrument for other purpose different to what it was designed, and > household MW oven were designed to heat and cook foods, not to process > tissues. > Besides that, all manufacturer now have a disclaimer stating that use of > the MW oven for purposes other than they are intended to, void any > guarantees. > > The ONLY solution would be to use the household MW oven INSIDE a fume > hood. > The new CAP Anatomic Pathology Checklist for its Accreditation Program > ANP.29430 Microwave devices state that used OUTSIDE a fume hood should > have an INTEGRAL fume extractor that needs to be properly connected to a > house air handling system or approved stand alone laboratory air handling > system. > > I still think that, at least for tissue processing, the message stands > valid: "do not use them!" > By the way, this has nothing to do with any "popularity contest" that for > sure CAP will never win! > Ren? J. > > Joe Nocito wrote: > but what about using a table top fume absorber with charcoal filters? CAP > writes these questions so ambiguously that it becomes a joke. This is the > main reason why I have a negative attitude towards CAP. > > Joe > ----- Original Message ----- > From: "Rene J Buesa" > To: "Joe Nocito" ; "'Parker, Helayne'" > ; > Sent: Thursday, March 23, 2006 4:19 PM > Subject: RE: [Histonet] CAP Guidelines for Microwaves > > >> Joe: >> It is not external ventilation to the MW oven what CAP wants, is a vent >> connected from the microwave to an outlet and there is not a single >> household MW oven with that characteristec so, for all practical purposes >> the mensage keeps being: don't use them (the household appliance) for >> histology work, specially if tissue processing is the objective of the >> use. >> Ren? J. >> >> Joe Nocito wrote: >> I don't believe CAP is saying not to use them. They are saying use them >> safely with ventilation and make sure they don't leak radiation. >> >> -----Original Message----- >> From: histonet-bounces@lists.utsouthwestern.edu >> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J >> Buesa >> Sent: Thursday, March 23, 2006 11:35 AM >> To: Parker, Helayne; histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] CAP Guidelines for Microwaves >> >> Helayne: >> In short, the message from CAP is: "do not use them!" >> Unfortunate though! >> Ren? J. >> >> "Parker, Helayne" wrote: >> I was wondering if anyone new what CAP require of us if we have a common >> household microwave that we use for special stains. >> >> Helayne Parker,HT (ASCP) >> Histology Section Head >> Skaggs Community Health Center >> Branson, Missouri >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> >> --------------------------------- >> Blab-away for as little as 1?/min. Make PC-to-Phone Calls using Yahoo! >> Messenger with Voice. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> >> >> >> --------------------------------- >> Blab-away for as little as 1?/min. Make PC-to-Phone Calls using Yahoo! >> Messenger with Voice. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> -- >> No virus found in this incoming message. >> Checked by AVG Free Edition. >> Version: 7.1.385 / Virus Database: 268.3.0/290 - Release Date: 3/23/2006 >> >> > > > > > > > --------------------------------- > Yahoo! Messenger with Voice. PC-to-Phone calls for ridiculously low rates. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.385 / Virus Database: 268.3.1/292 - Release Date: 3/24/2006 > > From jnocito <@t> satx.rr.com Sun Mar 26 06:17:06 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sun Mar 26 06:17:12 2006 Subject: [Histonet] Apology Message-ID: <008601c650cf$330dc2b0$e8bd0b43@yourxhtr8hvc4p> I apologize for using improper language in my first posting of venting the flame cabinets posted last week. I've been preparing my lab for a move into a new location, plus studying for my Pathologist Assistant's exam. Working long hours and studying has lowered my professionalism and has stained my reputation. I hope you all can find it in your hearts and minds to forgive me. I have unintentionally offended some people due to fatigue. My defenses were down. I have learned my lesson. In the future I will not post anything to the Histonet when I am in the condition I am currently in. Thank you. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX From jnocito <@t> satx.rr.com Sun Mar 26 06:21:13 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sun Mar 26 06:21:18 2006 Subject: [Histonet] Xylene exposure References: <20060325151617.49980.qmail@web30705.mail.mud.yahoo.com> Message-ID: <00a001c650cf$c633e560$e8bd0b43@yourxhtr8hvc4p> Jeannie, we do not let any pregnant employee near xylene nor formalin. I had two pregnant employees due at the same time. I was able to set up to cutting stations in a different location. They cut surgicals, recuts, special, immunos and did the paper work. It was tough during maternity leave, but the pathologists were understanding. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Jeannie Heck" To: "Histonet e-mail ?'s" Sent: Saturday, March 25, 2006 9:16 AM Subject: [Histonet] Xylene exposure > What special precautions, if any, does your facility > make for pregnant employees whose job duties involve > the use of, or exposure to xylene? > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.385 / Virus Database: 268.3.1/292 - Release Date: 3/24/2006 > > From Jackie.O'Connor <@t> abbott.com Sun Mar 26 15:55:02 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Sun Mar 26 15:55:28 2006 Subject: [Histonet] Xylene exposure Message-ID: Is there a new 'atmosphere' regarding pregnant women in histology? Is there new info on xylene and formalin dangers to fetuses? What about other histo chemicals, i.e., silver nitrate? Methanol? Aniline dyes? I'm not challenging this information - I'm just curious if there is something new I haven't heard about. Jackie O' "Joe Nocito" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/26/2006 06:21 AM To: "Jeannie Heck" , "Histonet e-mail ?'s" cc: (bcc: Jackie M O'Connor/LAKE/GPRD/ABBOTT) Subject: Re: [Histonet] Xylene exposure Jeannie, we do not let any pregnant employee near xylene nor formalin. I had two pregnant employees due at the same time. I was able to set up to cutting stations in a different location. They cut surgicals, recuts, special, immunos and did the paper work. It was tough during maternity leave, but the pathologists were understanding. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Jeannie Heck" To: "Histonet e-mail ?'s" Sent: Saturday, March 25, 2006 9:16 AM Subject: [Histonet] Xylene exposure > What special precautions, if any, does your facility > make for pregnant employees whose job duties involve > the use of, or exposure to xylene? > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.385 / Virus Database: 268.3.1/292 - Release Date: 3/24/2006 > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Mar 26 18:42:16 2006 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Sun Mar 26 18:42:12 2006 Subject: [Histonet] RE:H&E on GMA sections In-Reply-To: Message-ID: <200603270042.k2R0fucx072795@pro12.abac.com> Leslie, You are going to have a hard time getting 1-2 micron GMA sections to look just like 4-6 micron ffpe sections with H&E. Besides the obvious thickness difference the stain has to pass thru the GMA as it is not removed. In my experience this takes longer with more concentrated stain. I use Gill's#3 hematoxylin for 15min. wash in tap h20 to achieve bluing. I use a commercial alcoholic eosin/phloxine. After Hematox. And wash I airdry the sections standing up (10-20 min. in my dry climate) I then dip briefly in 95% alcohol, dip about 90 times (I keep it moving) in the Eosin, then quickly thru 2 chgs. Of 95 ETOH, about 5 dips ea., then 2 chgs. Of 100%etoh, once you get past the alcohol with water in it you can slow down, it is the water/alcohol mix that causes GMA to lift off the slide in my experience, I usually go into 100 about 1 min. ea chg then in 3 xylenes and permount. This is the only stain I do for GMA that I don't airdry the sections and coverslip with just a brief dip in xylene, the reason I use alcohol for the HE is because I use an alcoholic eosin, even when I use aqueous eosin I go thru Etoh (albeit quickly) to get the differentiation of the eosin we desire. Every other stain I do for GMA, if it ends in water, I airdry the section, dip once in xylene and permount. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carl Hobbs Sent: Saturday, March 25, 2006 11:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE:H&E on GMA sections Well.....after leaving my slide-mounted GMA-embedded sections in a 60C oven for an hour. I'll rinse them in tapwater and then leave them in Hx for about 20mins. After that, I'll rinse them in tapwater, have a look down the m'scope to note the intensity of Hx staining and differentiate in 0.5% HCL in 70% Alc ( water if you want)until I think that the balance is good. Then I will leave in 0.5% Eosin for as long as I want ( say, 20mins). Then wash in tapwater until I achieve the best Hx-Eosin balance. I then blot dry my resin sections and place them in the same oven to dry...min an hour. Then put them in xylene and , after 15-odd mins ..mount in DPX! No times are critical, IMHO. That's the great thing about H&E....just get a routine that is reproducable/consistent for your lab. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: None To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 28, Issue 41 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Staining Glycol Methacrylate (Leslie D Duncan) 2. Ventana IVIEW Detection Kits (Tracey.Lenek@CLS.ab.ca) 3. endothelial marker FFPE Rat (Steven Coakley) 4. RE: anti-human TNFR-1 anti-mouse TNFR-1 and 2 (Guillermo Palao) 5. RE: endothelial marker FFPE Rat (Elizabeth Chlipala) 6. RE: Ventana IVIEW Detection Kits (Sebree Linda A.) 7. peripheral axons in soft tissue (Elizabeth Chlipala) 8. RE: endothelial marker FFPE Rat (Trajkovic, Dusko) 9. Job openings in Michigan (Mary McCoy) 10. RE: peripheral axons in soft tissue (Smith, Allen) 11. RE: Input on Milestone RHS-1 and RHS-2 (Justin Thomas) 12. Autodial alarms (Marian powers) 13. Hybrid capture, CISH (Katia Cristina Catunda) 14. Xylene exposure (Jeannie Heck) 15. Re: Xylene exposure (Rene J Buesa) ---------------------------------------------------------------------- Message: 1 Date: Fri, 24 Mar 2006 12:28:22 -0600 From: Leslie D Duncan Subject: [Histonet] Staining Glycol Methacrylate To: histonet@lists.utsouthwestern.edu Message-ID: <44243A46.3050508@bms.com> Content-Type: text/plain; charset="iso-8859-1" Hi, I am experimenting with glycol methacrylate embedded rodent testes. I am looking for a good H&E staining protocol to use that will give results comparable to paraffin-embedded tissue (per request of my management). We have dehydrated on the tissue processor to 100% ethanol, ilfiltrated and embedded with JB-4, and sectioned at 1-2 microns. The sections are placed on Poly L-lysine slides. Any protocols, ideas, and/or suggestions will be greatly appreciated! Thank you, Leslie Duncan Bristol-Myers Squibb, PRI ------------------------------ Message: 2 Date: Fri, 24 Mar 2006 11:35:08 -0700 From: Subject: [Histonet] Ventana IVIEW Detection Kits To: Message-ID: <4BC300747AF87A48BCDF8E48BC2885CE02C1D598@mail1.calgary.com> Content-Type: text/plain; charset="iso-8859-1" Just a general question for all the Ventana automated IHC equipment users - this time last year we found that the IVIEW detection kits were overstaining. Now we are experiencing weak staining. Has anyone else found variabilities with the staining in their kits? We have relayed our issues to Ventana but as usual are the only ones complaining. Tracey CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify the sender immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. ------------------------------ Message: 3 Date: Fri, 24 Mar 2006 10:08:33 -0800 (PST) From: Steven Coakley Subject: [Histonet] endothelial marker FFPE Rat To: Histonet@lists.utsouthwestern.edu Message-ID: <20060324180833.66058.qmail@web38202.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Has anyone any knowledge of any endothelial markers, such as CD31, RECA, ect.... for FFPE for a rat muscle angiogenesis study. Steve --------------------------------- Blab-away for as little as 1"/min. Make PC-to-Phone Calls using Yahoo! Messenger with Voice. ------------------------------ Message: 4 Date: Fri, 24 Mar 2006 20:12:57 +0100 (CET) From: Guillermo Palao Subject: [Histonet] RE: anti-human TNFR-1 anti-mouse TNFR-1 and 2 To: Histonet Message-ID: <20060324191258.74730.qmail@web26210.mail.ukl.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Again, any suggestions for good commercially available antibody for mouse TNRs 1 and 2 (CD120 a,b) are most welcome. Thanks for all previous and future help in saving time with painful optimizations of non-working antibodies. Guillermo Alessandro Serra escribis: Dear All Has anybody used succesfully any commercially available anti-human TNFR-1 (CD120a)? If yes, could you please be so kind to tell me which one? Thank you very much for your help Alessandro Serra, BSc. --------------------------------- LLama Gratis a cualquier PC del Mundo. Llamadas a fijos y msviles desde 1 cintimo por minuto. http://es.voice.yahoo.com ------------------------------ Message: 5 Date: Fri, 24 Mar 2006 12:31:52 -0700 From: "Elizabeth Chlipala" Subject: RE: [Histonet] endothelial marker FFPE Rat To: "'Steven Coakley'" , Message-ID: <000a01c64f79$9a72f4e0$0300a8c0@Chlipala> Content-Type: text/plain; charset="iso-8859-1" Steve I have used the goat-anti mouse PECAM-1 from santa cruz for rat tissue and it works. The only thing is that I happen to have the older lot number that works in FFPE tissues. I don't know what newer lots will do. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Coakley Sent: Friday, March 24, 2006 11:09 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] endothelial marker FFPE Rat Has anyone any knowledge of any endothelial markers, such as CD31, RECA, ect.... for FFPE for a rat muscle angiogenesis study. Steve --------------------------------- Blab-away for as little as 1"/min. Make PC-to-Phone Calls using Yahoo! Messenger with Voice. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 24 Mar 2006 13:36:22 -0600 From: "Sebree Linda A." Subject: RE: [Histonet] Ventana IVIEW Detection Kits To: , Message-ID: Content-Type: text/plain; charset="US-ASCII" We have not experienced that phenomenon ever that I can recall. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tracey.Lenek@CLS.ab.ca Sent: Friday, March 24, 2006 12:35 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana IVIEW Detection Kits Just a general question for all the Ventana automated IHC equipment users - this time last year we found that the IVIEW detection kits were overstaining. Now we are experiencing weak staining. Has anyone else found variabilities with the staining in their kits? We have relayed our issues to Ventana but as usual are the only ones complaining. Tracey CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify the sender immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Fri, 24 Mar 2006 12:40:43 -0700 From: "Elizabeth Chlipala" Subject: [Histonet] peripheral axons in soft tissue To: Message-ID: <000001c64f7a$d6a91f10$0300a8c0@Chlipala> Content-Type: text/plain; charset="us-ascii" Hello Everyone Is there a stain that I can use that will detect peripheral nerve (axons) in soft tissue. I have checked around a bit and it looks like performing a Bodian would work. I would appreciate any input or advice on this. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 ------------------------------ Message: 8 Date: Fri, 24 Mar 2006 11:46:35 -0800 From: "Trajkovic, Dusko" Subject: RE: [Histonet] endothelial marker FFPE Rat To: "Steven Coakley" , Message-ID: <3AD0BD3142459B4E9B12CBEAFF2B89B202A94F4F@lajamrexm01.amer.pfizer.com> Content-Type: text/plain; charset="iso-8859-1" I had Santa Cruz goat antibody PECAM-1 sc-1505 (lot# G1103) that worked well on rat tissue, however the new lot number (C2405) which I received last week, is not working well at all. I will be getting a new lot number from them next Tuesday. I will post my results later in the week. Dusko Trajkovic -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Friday, March 24, 2006 11:32 AM To: 'Steven Coakley'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] endothelial marker FFPE Rat Steve I have used the goat-anti mouse PECAM-1 from santa cruz for rat tissue and it works. The only thing is that I happen to have the older lot number that works in FFPE tissues. I don't know what newer lots will do. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven Coakley Sent: Friday, March 24, 2006 11:09 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] endothelial marker FFPE Rat Has anyone any knowledge of any endothelial markers, such as CD31, RECA, ect.... for FFPE for a rat muscle angiogenesis study. Steve --------------------------------- Blab-away for as little as 1"/min. Make PC-to-Phone Calls using Yahoo! Messenger with Voice. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately. ------------------------------ Message: 9 Date: Fri, 24 Mar 2006 15:18:53 -0500 From: "Mary McCoy" Subject: [Histonet] Job openings in Michigan To: histonet@lists.utsouthwestern.edu Cc: Marianna Cerecke Message-ID: Content-Type: text/plain; charset=us-ascii Lakeland Regional Health System Position: Histotechnologist Salary: Competitive Place of work: Lakeland Hospital, St. Joseph, MI Position status: Two, full-time permanent positions Number of Vacant Positions: 02 Description of position: Performs technical functions necessary in preparation of tissues for microscopic examination by the pathologist; assists the coordinator with quality assurance monitoring; management of the immunohistochemical (IHC) stains and associated quality control; assists the coordinator with the implementation of new procedures and the evaluation of new instruments; and assists the coordinator with training as necessary. HT or HTL (ASCP) certification is preferred; we will consider a Bachelors degree in a related field and training in the department. Lakeland offers a comprehensive benefits package, competitive salaries, PTO, educational reimbursement, and much more. Lakeland is located on the shores of beautiful Lake Michigan and is just 90 minutes from Chicago. Please send cover letter and resume to Marianna Cerecke, Lakeland Regional Health System, 6418 Deans Hill Rd, Berrien Center, MI 49102. Or fax to: 269-473-3069. For questions call 269-473-3069 or visit our website at www.lakelandhealth.org to apply on-line. ------------------------------ Message: 10 Date: Fri, 24 Mar 2006 16:08:38 -0500 From: "Smith, Allen" Subject: RE: [Histonet] peripheral axons in soft tissue To: "Elizabeth Chlipala" Cc: histonet@lists.utsouthwestern.edu Message-ID: <5D2189E74151CC42BEC02906BA8996322B91AD@exchsrv01.barrynet.barry.edu> Content-Type: text/plain; charset="us-ascii" In my hands, the most reliable technique for demonstrating peripheral nerves is Kiernan's physical developer method. [pp. 371-372 of the 3rd edition of J.A. Kiernan's HISTOLOGICAL AND HISTOCHEMICAL METHODS.] Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elizabeth Chlipala Sent: Friday, March 24, 2006 2:41 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] peripheral axons in soft tissue Hello Everyone Is there a stain that I can use that will detect peripheral nerve (axons) in soft tissue. I have checked around a bit and it looks like performing a Bodian would work. I would appreciate any input or advice on this. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) ------------------------------ Message: 11 Date: Fri, 24 Mar 2006 13:37:00 -0800 (PST) From: Justin Thomas Subject: [Histonet] RE: Input on Milestone RHS-1 and RHS-2 To: histonet@lists.utsouthwestern.edu Message-ID: <20060324213700.4795.qmail@web35704.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 is anyone familiar with the milestone RHS-1 and RHS-2 microwaves? If so, what are your pros and cons for the two units. Thanks in advance for any input! -thomas --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2"/min or less. ------------------------------ Message: 12 Date: Fri, 24 Mar 2006 17:27:07 -0500 From: "Marian powers" Subject: [Histonet] Autodial alarms To: Message-ID: <000801c64f92$163b1c70$be4dff45@MEGAN> Content-Type: text/plain; charset="iso-8859-1" Looking for an autodial alarm for a tissue processor, any recommendations? Thanks in advance. Marian ------------------------------ Message: 13 Date: Fri, 24 Mar 2006 21:08:12 -0300 From: "Katia Cristina Catunda" Subject: [Histonet] Hybrid capture, CISH To: Message-ID: <004401c64fa0$35052890$17d0fea9@privatexx> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Dear collegues, I would like some information about vendors for Hybrid Capture equipments and supplies as for CISH, can anybody help me (just know Digene and Zymer respectively). And how about some tips for Hybridizators? We've found from boekel and zymed with a great price diferenciation. Thanks Katia ------------------------------ Message: 14 Date: Sat, 25 Mar 2006 07:16:17 -0800 (PST) From: Jeannie Heck Subject: [Histonet] Xylene exposure To: Histonet e-mail ?'s Message-ID: <20060325151617.49980.qmail@web30705.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 What special precautions, if any, does your facility make for pregnant employees whose job duties involve the use of, or exposure to xylene? __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com ------------------------------ Message: 15 Date: Sat, 25 Mar 2006 09:29:41 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Xylene exposure To: Jeannie Heck , Histonet e-mail ?'s Message-ID: <20060325172941.74110.qmail@web61213.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Three in my staff confronted that situation (not simultaneously!!) and I tried to rotate each to tasks with much less exposure. On the other hand our monitoring system always came as "0" (13 ppm / hour) which is approximately 9% for xylene STEL and 13% for xylene TWA exposure limits. I always found that reasignment of tasks is the best solution but it requires a strongly implemented crosstraining program. Hope this will help! Reni J. Jeannie Heck wrote: What special precautions, if any, does your facility make for pregnant employees whose job duties involve the use of, or exposure to xylene? __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- New Yahoo! Messenger with Voice. Call regular phones from your PC and save big. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 28, Issue 41 **************************************** -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.385 / Virus Database: 268.2.6/287 - Release Date: 21/03/2006 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Sun Mar 26 18:52:11 2006 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Sun Mar 26 18:53:19 2006 Subject: [Histonet] CD11b In-Reply-To: Message-ID: <200603270051.k2R0pqin076164@pro12.abac.com> Jo, I tried several cd11b's on ffpe tissue to no avail, I switched to F4/80 clone for macs and it does work on ffpe. The one I have is rat anti ms I believe, what species are you trying to label? Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Mauger Sent: Friday, March 24, 2006 9:12 AM To: mariaforzan@hotmail.com; histonet@pathology.swmed.edu; ploykasek@phenopath.com Subject: [Histonet] CD11b Hi All, Does anyone know of a CD11b antibody that works on FFPE? Thanks, Jo >>> Patti Loykasek 03/24/06 11:07 AM >>> Maria, We are using a different clone - clone G3 from Chemicon. In our validation we did see some cross reactivity with basophils. It was positive on all cases of mast cell disease we tested & negative on other neoplasms & hematopoietic cell types. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Hello! > > We are having some trouble with our IHC stain for mast cell tryptase > (Dako AA1 clone). We seem to get granular intracytoplasmic staining > in several cells that are probably not mast cells but histiocytes or > neoplastic plasma cells. I know that the antibody is very specific > for mast cells and should not label anything else. So, does anybody > have any ideas? Could it be the retrieval? Or the secondary? We use > EnVision so it should not be a problem. > > Does anybody have any suggestions or has encounter a similar problem? > > Maria Forzan > > Finn Pathologists > > 44 (0)1379 854180 > > "Pebbles are inevitable" > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Sun Mar 26 18:57:06 2006 From: tkngflght <@t> yahoo.com (Cheryl) Date: Sun Mar 26 18:57:10 2006 Subject: [Histonet] Xylene, unborn babies and a parallel note about thyroid function in female histotechs In-Reply-To: Message-ID: <20060327005706.1002.qmail@web50914.mail.yahoo.com> In considering precautions for mom, I think it's more of an issue of why take the risk if it's not necessary regardless of what OSHA and all the other regulating bodies say about exposure. We each can decide as an individual to stay or go in any lab, or wear a respirator or not, an unborn fetus cannot. After the fact is too late should there BE some grounds for caution. I have carried a baby while working in a lab, wore a negative pressure respirator whenever exposure was unavoidable, and was GREATFUL to my peers for exchanging duties with me when pregnant--and my girl was born a full month early. Liability for exposures and problems caused during gestation have a 19 year liability duration. Who's to say what the regulations will be a few years from now? So from the perspective of both the health of the baby and sound management of the lab, caution is the better part of wise management. Balance is the key. There are preventable exposures. There are good working practices, personal protective wear and mechanical safety precautions that everyone should observe. There are also unavoidable risks. I'm not suggesting wrapping the mom-to-be up in aluminum foil and having her stand in the fume hood for nine months. In all of my labs when someone was pregnant, we rotated them out of changing machines (in addition to the fumes there were lifting issues) and did not require them to change the recyclers. If we couldn't (or the lab wasn't terribly 'clean') we provided respirators that were comfortable and changed the cannisters often, even through the months of feeding the little one after birth. I remember making these accommodations over 20 years ago (my girl is 10)...taking care of the people is what we are supposed to do. Think about what labs were like 20 years ago...open vats of xylene and bare hands. Xylene soaked rags for cleaning all the countertops and hot xylene to speed things up. But now we know better, don't we? It might not be something you'd be aware of now, but consider 15 or 20 years down the line when researchers prove (hypothetically) that all women who carried children while working in chemical environments, breathing low levels of xylene and formalin, caused their children to lose 10 or 20 points of IQ? I'd not sleep well at night. Have you ever polled large groups of techs who've worked in histo over 10 years--long enough to be before good clean air regs? Half of the women have some sort of thyroid disorder (it is just women--higher body fat levels) ...hypo or hyper or Hashi's or something...there is NO documentation of the effect of xylene on thyroid function...but there will be someday. If you hesitate to institute changes to accommodate your mom-to-be, poll your employees. They will rise to the occasion and surprise you in what they are willing to do for each other. Best wishes for a happy and healthy new histo-baby!! Cheryl Jackie M O'Connor wrote: Is there a new 'atmosphere' regarding pregnant women in histology? Is there new info on xylene and formalin dangers to fetuses? What about other histo chemicals, i.e., silver nitrate? Methanol? Aniline dyes? I'm not challenging this information - I'm just curious if there is something new I haven't heard about. Jackie O' "Joe Nocito" Sent by: histonet-bounces@lists.utsouthwestern.edu 03/26/2006 06:21 AM To: "Jeannie Heck" , "Histonet e-mail ?'s" cc: (bcc: Jackie M O'Connor/LAKE/GPRD/ABBOTT) Subject: Re: [Histonet] Xylene exposure Jeannie, we do not let any pregnant employee near xylene nor formalin. I had two pregnant employees due at the same time. I was able to set up to cutting stations in a different location. They cut surgicals, recuts, special, immunos and did the paper work. It was tough during maternity leave, but the pathologists were understanding. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Jeannie Heck" To: "Histonet e-mail ?'s" Sent: Saturday, March 25, 2006 9:16 AM Subject: [Histonet] Xylene exposure > What special precautions, if any, does your facility > make for pregnant employees whose job duties involve > the use of, or exposure to xylene? > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.385 / Virus Database: 268.3.1/292 - Release Date: 3/24/2006 > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PKamalavenkatesh <@t> wockhardtin.com Sun Mar 26 22:17:36 2006 From: PKamalavenkatesh <@t> wockhardtin.com (PKamalavenkatesh@wockhardtin.com) Date: Sun Mar 26 22:12:42 2006 Subject: [Histonet] Kwik-Diff stain kit (Cat No. 9990702)- procedure reg Message-ID: Dear Members, We have recently procured Kwik-Diff stain kit (Cat No. 9990702) from thermo Shandon. Unfortunately, we haven?t received the product insert and staining procedure from the manufacturers. Our repetitive communications with their Indian agent resulted no progress in this issue. It would be grateful if any of our histonetters who are using this stain kit can get us the staining procedure for the same. REGARDS Dr.P.Kamalavenkatesh Pre clinical safety evaluation division New Drug Discovery- Biology Wockhardt Research Center E.mail : Pkamalavenkatesh@wockhardtin.com Please Visit our New Corporate Web Site www.wockhardt.com --------------------- Disclaimer ------------------------------------------------------------------------------ Information transmitted by this E-MAIL is proprietary to Wockhardt Ltd. and/ or its Group Companies and/or,its Customers and is intended for use only by the individual or entity to which it is addressed, and may contain information that is privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. --------------------------------------------------------------------------------------------------------------- From kemlo.rogerson <@t> waht.swest.nhs.uk Mon Mar 27 01:31:40 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Mar 27 01:31:01 2006 Subject: [Histonet] Kwik-Diff stain kit (Cat No. 9990702)- procedure r eg Message-ID: If memory serves you have a pale blue fixative, 30s with dipping, then an orange one, 30s with dipping, then a dark blue one, 30s with dipping. Wash off with water then voila. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 "Following the line of least resistance makes both men and rivers crooked" This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: PKamalavenkatesh@wockhardtin.com [mailto:PKamalavenkatesh@wockhardtin.com] Sent: Monday, March 27, 2006 5:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Kwik-Diff stain kit (Cat No. 9990702)- procedure reg Dear Members, We have recently procured Kwik-Diff stain kit (Cat No. 9990702) from thermo Shandon. Unfortunately, we haven't received the product insert and staining procedure from the manufacturers. Our repetitive communications with their Indian agent resulted no progress in this issue. It would be grateful if any of our histonetters who are using this stain kit can get us the staining procedure for the same. REGARDS Dr.P.Kamalavenkatesh Pre clinical safety evaluation division New Drug Discovery- Biology Wockhardt Research Center E.mail : Pkamalavenkatesh@wockhardtin.com Please Visit our New Corporate Web Site www.wockhardt.com --------------------- Disclaimer ---------------------------------------------------------------------------- -- Information transmitted by this E-MAIL is proprietary to Wockhardt Ltd. and/ or its Group Companies and/or,its Customers and is intended for use only by the individual or entity to which it is addressed, and may contain information that is privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. ---------------------------------------------------------------------------- ----------------------------------- From JurciseJ <@t> pediatrics.ohio-state.edu Mon Mar 27 06:26:49 2006 From: JurciseJ <@t> pediatrics.ohio-state.edu (Jurcisek, Joseph) Date: Mon Mar 27 06:28:00 2006 Subject: [Histonet] Staining Glycol Methacrylate Message-ID: <714B9F12B4E18C4C843B66E8E190F2ADB1ED59@res2k3ms01.CRII.ORG> Leslie, I used to do a lot of JB-4 work. We had an HEA stain (Hematoxylin, Eosin, Azure) that we really liked. Here is the protocol Reagents Hematoxylin (we used Harris) Eosin 0.5% buffered Eosin (buffered in 0.01M phosphate buffer pH 5) Azure 0.1% buffered Azure II (buffered in phosphate buffer pH2.8) Procedure Hematoxylin 1.5 min tap water rinse Eosin 20 seconds di H2O rinse di H2O rinse Azure 1.5-2 min di H2O rinse di H2O rinse Ethylene Glycol monomethyl ether 1 quick dip di H2O rinse Since JB-4 is miscible in water, there is no need to hydrate through graded alcohols. I also have a staining protocol that used microwaves and create a very vibrant stain. Unfortunately it is buried in ancient files right now and will require some digging. If you are interested, let me know and I will do it. Joe Joseph A. Jurcisek Senior Research Associate Center for Microbial Pathogenesis Columbus Children's Research Institute 700 Children's Dr. Columbus, OH 43205-2696 (614) 355-3521 fax (614) 722-2818 jurcisej@ccri.net -----Original Message----- From: Leslie D Duncan [mailto:leslie.duncan@bms.com] Sent: Friday, March 24, 2006 1:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Staining Glycol Methacrylate Hi, I am experimenting with glycol methacrylate embedded rodent testes. I am looking for a good H&E staining protocol to use that will give results comparable to paraffin-embedded tissue (per request of my management). We have dehydrated on the tissue processor to 100% ethanol, ilfiltrated and embedded with JB-4, and sectioned at 1-2 microns. The sections are placed on Poly L-lysine slides. Any protocols, ideas, and/or suggestions will be greatly appreciated! Thank you, Leslie Duncan Bristol-Myers Squibb, PRI From MAUGER <@t> email.chop.edu Mon Mar 27 07:19:07 2006 From: MAUGER <@t> email.chop.edu (Joanne Mauger) Date: Mon Mar 27 07:19:45 2006 Subject: [Histonet] CD11b Message-ID: Patsy, I'm staining human tissue. Where do you get the F4/80? Thanks, Jo >>> "patsy ruegg" 03/26/06 7:52 PM >>> Jo, I tried several cd11b's on ffpe tissue to no avail, I switched to F4/80 clone for macs and it does work on ffpe. The one I have is rat anti ms I believe, what species are you trying to label? Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joanne Mauger Sent: Friday, March 24, 2006 9:12 AM To: mariaforzan@hotmail.com; histonet@pathology.swmed.edu; ploykasek@phenopath.com Subject: [Histonet] CD11b Hi All, Does anyone know of a CD11b antibody that works on FFPE? Thanks, Jo >>> Patti Loykasek 03/24/06 11:07 AM >>> Maria, We are using a different clone - clone G3 from Chemicon. In our validation we did see some cross reactivity with basophils. It was positive on all cases of mast cell disease we tested & negative on other neoplasms & hematopoietic cell types. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Hello! > > We are having some trouble with our IHC stain for mast cell tryptase > (Dako AA1 clone). We seem to get granular intracytoplasmic staining > in several cells that are probably not mast cells but histiocytes or > neoplastic plasma cells. I know that the antibody is very specific > for mast cells and should not label anything else. So, does anybody > have any ideas? Could it be the retrieval? Or the secondary? We use > EnVision so it should not be a problem. > > Does anybody have any suggestions or has encounter a similar problem? > > Maria Forzan > > Finn Pathologists > > 44 (0)1379 854180 > > "Pebbles are inevitable" > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jacqueline.Malam <@t> rli.mbht.nhs.uk Mon Mar 27 07:23:33 2006 From: Jacqueline.Malam <@t> rli.mbht.nhs.uk (Malam Jacqueline) Date: Mon Mar 27 07:23:59 2006 Subject: [Histonet] iView detection kits Message-ID: Just occasionally I have noticed a slight variation; but more with the haematoxylin 1 reagent Jacqui Malam Lancaster UK DISCLAIMER: This e-mail is confidential and privileged. If you are not the intended recipient please accept our apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform postmaster@rli.mbht.nhs.uk that this message has gone astray before deleting it. Comments or opinions expressed in this email are those of their respective contributors only. The views expressed do not represent the views of the Trust, its management or employees. Morecambe Bay Hospitals NHS Trust is not responsible and disclaims any and all liability for the content of comments written within.Thank you for your co-operation. From ROrr <@t> enh.org Mon Mar 27 07:38:05 2006 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Mon Mar 27 07:38:10 2006 Subject: [Histonet] I view kits Message-ID: Hi Tracey, I use the IVIEW kits on a Benchmark (still praying for an XT to drop from heaven). I have not seen anything that you mention in the lot- to- lot consistency of these kits. As much as it might be the kits, it MAY be something else on the instrument that is affecting your results. My first question would be if you are seeing light results on EVERY stain? If you are seeing normal staining on ANY slide on the run I would tend to R/o the kit. If all stains look light, I would have the instrument checked over, you might have either a clog or a leak somewhere. Does the XT still have vortex mixers? If so those might be clogged. Is the staining uniformly light across the slide or are you seeing Patchy staining, with some areas staining ok and other areas light? This could be a bunch of things unrelated to the instrument... I'll be happy to troubleshoot with you privately to see if we can nail down the problem. Or I would recommend contacting the in house tech support at Ventana, they can help you through this. Another person in Canada is Linda Elliott, she used to work at Credit Valley, I'm not sure if she is still there, but she has a lot of experience with the Ventana kits. Hope this helps, Becky Becky Orr CLA,HT(ASCP) IHC Lead Evanston Northwestern Healthcare 847-570-2771 Message: 2 Date: Fri, 24 Mar 2006 11:35:08 -0700 From: Subject: [Histonet] Ventana IVIEW Detection Kits To: Message-ID: <4BC300747AF87A48BCDF8E48BC2885CE02C1D598@mail1.calgary.com> Content-Type: text/plain; charset="iso-8859-1" Just a general question for all the Ventana automated IHC equipment users - this time last year we found that the IVIEW detection kits were overstaining. Now we are experiencing weak staining. Has anyone else found variabilities with the staining in their kits? We have relayed our issues to Ventana but as usual are the only ones complaining. Tracey From LINDA.MARGRAF <@t> childrens.com Mon Mar 27 08:42:34 2006 From: LINDA.MARGRAF <@t> childrens.com (LINDA MARGRAF) Date: Mon Mar 27 08:42:58 2006 Subject: [Histonet] Re: Help with the Histonet, please help! Message-ID: Dear John ( and other Histonetters please take note): The reason your email did not go through the Histonet system out to the membership is that your email address on the list of subscribers differs from the address you sent your message from. The server will only send messages out from members whose address matches EXACTLY the one on the list. In your case John, the address on the list is ..@med.va.gov and not ..@va.gov as your message below shows. We block all mail from addresses not on our subscriber list to stop as much spam as possible (I clear out a lot of spam every day from the held mail). Anyway, to fix this you need to subscribe the new address to the list. If your email system randomly uses both addresses you can subscribe both but block the mail coming to you from one address so you don't get duplicate mail every day. Go to http://lists.utsouthwestern.edu/mailman/listinfo/histonet to change your address. Sorry it took a couple of days to respond. I am the only one who administers this list on a daily basis and I was out of town. Linda M Histonet administrator >>> "Thweatt, John T" 03/23/06 10:14 AM >>> Dear Histonet, I am having a little trouble of a technical nature that I am wondering if you could look into. The service is great and the response to questions is sometimes overwhelming. Recently my supervisor had a question that he wanted me to inquire about and naturally I turned to the histonet for assistance as it is a wonderful resource for us in this field. But when I sent in my inquiry, I received this strange message about "my inquiry is being reviewed by a moderator as I am a `non` member" What is that all about? I had not seen that before. Is this a new layer or something? I had been under the impression that I was a member of the group and I can post and messages would go through without this extra step. Can someone tell me what is going on? And what must I do to get my status back to the way it was when I can post without any issues? I would not post anything that is negative, derogatory or inflammatory to any of the members, so I cannot understand what has happened. I just need some help and maybe some answers. Your help is appreciated. Sincerely, ************************************ Tom Thweatt, HT (ASCP) Amarillo VA Health Care System Pathology and Laboratory Medicine Service Anatomical Pathology 6010 Amarillo Boulevard West Amarillo, TX 79106 806-355-9703 x 7071 fax 806-354-7865 <> From AFeatherstone <@t> KaleidaHealth.Org Mon Mar 27 08:47:26 2006 From: AFeatherstone <@t> KaleidaHealth.Org (Featherstone, Annette) Date: Mon Mar 27 08:47:39 2006 Subject: [Histonet] Cytochrome Oxidase Stain Message-ID: <9B4A77DF11463E4FB723D484214AE9BC26BECE@KALEXMB02.KaleidaHealth.org> PROCEDURE: Fixation: frozen tissue Technique: Unfixed frozen section at 10 microns thick. Quality Control: Muscle tissue Solutions: Reagents: 1. 0.1 M Sodium Acetate buffer pH 5.6 Sodium Acetate Trihydrate--------------------------1.360g Distilled water------------------------------------------100ml Adjust pH to 5.6 with acetic acid. Store in refrigerator. 2. 1% Manganese Chloride Manganese chloride------------------------------------1.0g Distilled water-------------------------------------------100ml 3. 0.1% Hydrogen Peroxide 30% Hydrogen peroxide---------------------------------0.1ml (50ul) Distilled water---------------------------------------------29.9ml (14.95) Mix just before use. 4. 1% Cupric Sulfate Cupric Sulfate---------------------------------------------1g Distilled water---------------------------------------------100ml 5. 1.0 N Sodium Hydroxide Sodium Hydroxide--------------------------------------4g Distilled water-------------------------------------------100ml 6. Incubating medium DAB-------------------------------------------------------0.03g 0.1 M sodium acetate buffer--------------------------13.5ml 1% manganese chloride--------------------------------1.5ml 0.1% hydrogen peroxide-------------------------------0.15ml Mix together just before use. Adjust pH to 5.5 with NaOH. Filter before use. Procedure: 1. Cut frozen sections at 10 microns. 2. Pour incubating solution over slides. Cover to prevent evaporation. 3. Incubate in 37?C oven for 1 hour. 4. Rinse in two changes distilled water, 5-10 seconds each. 5. Place in 1% cupric sulfate for 5 minutes. 6. Rinse in distilled water, 2-3 changes, 30 seconds total. 7. Dehydrate through graded alcohols, clear in xylene and coverslip. Results: Mitochondria------------------------------Brown Type I fibers-------------------------------Darker brown Type II fibers------------------------------Lighter brown Myofibrils----------------------------------Unstained DISTRIBUTION: This policy shall be distributed to all pathologists, technical and secretarial staff of the Department of Pathology and Laboratory Medicine. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Thursday, March 23, 2006 11:14 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 28, Issue 37 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: CAP - ANP.11713 Phase II (Weems, Joyce) 2. guidelines for Her2 i nterpretation (Goodwin, Diana) 3. RE: CAP - ANP.11713 Phase II (Horn, Hazel V) 4. The MT thing (Orr, Rebecca) 5. DAB in tablet form (meint002) 6. Fixation sensitive IHC markers (Thomas Crowell) 7. RE: Fixation sensitive IHC markers (Sebree Linda A.) 8. RE: CAP - ANP.11713 Phase II (Sebree Linda A.) 9. Re: DAB in tablet form (Andrea T. Hooper) 10. Re: guidelines for Her2 interpretation (Patti Loykasek) 11. p16 antibody (Dick Paulson [Source Medical Products]) 12. Contaminated Alcohols from VIP processors (bamoe@gundluth.org) 13. Avoiding Re: [Histonet] DAB in tablet form (Gayle Callis) 14. Re: DAB in tablet form (Rene J Buesa) 15. RE: Contaminated Alcohols from VIP processors (Charles.Embrey) 16. CAP Standard ANP.11713 - Phase II (Jeff Silverman) 17. RE: Fixation sensitive IHC markers (Luis Chiriboga) 18. RE: Contaminated Alcohols from VIP processors (Monfils, Paul) 19. National Search for Histology Supervisor at the National Cancer Institute; up to $84,559 per year (Nellis, Kevin Lee (NIH/NCI) [E]) 20. Re: Training Med Techs - some candid comments (Jennifer MacDonald) 21. MAK 6 HELP PLEASE!!!!!!!!!!! (Jesus Ellin) 22. Fw: Thermo/ Leica (MattG) 23. RE: Contaminated Alcohols from VIP processors (Bonner, Janet) 24. RE:JOB DESCRIPTION FOR HISTOLOGY ASSISTANT (mprice26@juno.com) ---------------------------------------------------------------------- Message: 1 Date: Wed, 22 Mar 2006 12:37:15 -0500 From: "Weems, Joyce" Subject: RE: [Histonet] CAP - ANP.11713 Phase II To: "Demarinis, Carolyn" , Message-ID: <83AACDB0810528418AA106F9AE9B7F7E01D34E34@sjhaexc02.sjha.org> Content-Type: text/plain; charset="US-ASCII" We give the control to the pathologist daily - who initials satisfactory or not... problems are dealt with by techs usually before path gets here tho. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Demarinis, Carolyn Sent: Wednesday, March 22, 2006 11:34 AM To: HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] CAP - ANP.11713 Phase II There is a new question on the CAP checklist: ANP.11713 - Phase II "Is there documented evidence of daily review of technical quality of histologic preparations by the pathologist?" Our present procedure is the supervisor/technologist documents daily review, but it is very clear that CAP is requiring the pathologist to initial a document daily proving they have reviewed the technical quality of slides. How are other hospitals satisfying this requirement? It seems crazy to make a pathologist initial a document daily, when it is evident they review hundreds of slides a day, and they would certainly report a problem to the supervisor when the stain is unacceptable. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. ------------------------------ Message: 2 Date: Wed, 22 Mar 2006 12:46:10 -0500 From: "Goodwin, Diana" Subject: [Histonet] guidelines for Her2 i nterpretation To: Message-ID: <80CDD9C3FEEAFD4982B114C4A6DFD00EE4A3D4@uphsmbx2.UPHS.PENNHEALTH.PRV> Content-Type: text/plain; charset="US-ASCII" I have been out of the loop with Her- 2 for a while, and the last I knew, it was left up to the individual labs to establish standards among the interpreting physicians for interpretation, pretty much based on the original Herceptest guidelines. Have any other standards been established? If so, would someone please provide me with a reference? Much obliged, Diana Goodwin Supervisor, Anatomic Pathology Pennsylvania Hospital Preston 655-C ph. 215-829-6532 fax 215-829-7564 e-mail goodwind@[pahosp.com ------------------------------ Message: 3 Date: Wed, 22 Mar 2006 11:54:56 -0600 From: "Horn, Hazel V" Subject: RE: [Histonet] CAP - ANP.11713 Phase II To: "Demarinis, Carolyn" , HISTONET@PATHOLOGY.SWMED.EDU Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C63038BDFFC@EMAIL.archildrens.org> Content-Type: text/plain; charset=us-ascii We send out a QC sheet daily with slides for the day. The docs sign off on this sheet noting the quality of the slides and return it to our lab. On the sheet it is just a matter of them checking off the slide review and if they are acceptable or not. HH -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Demarinis, Carolyn Sent: Wednesday, March 22, 2006 10:34 AM To: HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] CAP - ANP.11713 Phase II There is a new question on the CAP checklist: ANP.11713 - Phase II "Is there documented evidence of daily review of technical quality of histologic preparations by the pathologist?" Our present procedure is the supervisor/technologist documents daily review, but it is very clear that CAP is requiring the pathologist to initial a document daily proving they have reviewed the technical quality of slides. How are other hospitals satisfying this requirement? It seems crazy to make a pathologist initial a document daily, when it is evident they review hundreds of slides a day, and they would certainly report a problem to the supervisor when the stain is unacceptable. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== ------------------------------ Message: 4 Date: Wed, 22 Mar 2006 12:33:00 -0600 From: "Orr, Rebecca" Subject: [Histonet] The MT thing To: Message-ID: Content-Type: text/plain; charset="US-ASCII" AW I just love you guys! Everyone has good points and I appreciate Mike's input. The thing is I still don't have a supervisor and I'll be very sad to leave my cushy IHC queendom to go do it myself. Let's be done with this and move on. Becky Orr CLA,HT(ASCP) IHC Lead Evanston Northwestern Healthcare 847-570-2771 ------------------------------ Message: 5 Date: Wed, 22 Mar 2006 13:19:26 CST From: meint002 Subject: [Histonet] DAB in tablet form To: histonet@lists.utsouthwestern.edu Message-ID: <200603221919.k2MJJQ8t021230@vanguard.software.umn.edu> Content-Type: TEXT/plain; CHARSET=US-ASCII Dear Histos, Due to the hazards of working with 3,3' diaminobenzidine tetrahydrochloride (DAB) for muscle enzyme staining, I am considering purchasing DAB as either 5mg, or 10mg tablets. Some companies have them dissolve in water and others in a buffer solution. I am looking for input from anyone who has used these tablets. Do you like them, do they work well, staining results, which company, etc.... I know that you can also get DAB in sealed separate vials, but it is much more expensive and we are in an academic research setting where cost is a consideration. I am particularly interested in using them in the Cytochrome Oxidase stain. I have tried many procedures for this stain and my Dr. is not all that pleased with the results. If anyone has a good procedure for this stain, I would be grateful if you would send me a copy. I am revamping this lab and have had a lot of questions for the histonet that you have been very helpful in addressing. Thanks for all the suggestions in advance. Joyce Meints Histologist University of Minnesota Paul and Sheila Wellstone Muscular Dystrophy Center MMC 206 420 Washington Ave. SE Minneapolis, MN 55455-0392 e-mail: meint002@umn.edu Fax: 612-625-8488 lab phone: 612-626-4703 ------------------------------ Message: 6 Date: Wed, 22 Mar 2006 14:40:35 -0500 From: Thomas Crowell Subject: [Histonet] Fixation sensitive IHC markers To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Dear Histonetters, Can anyone suggest an antibody that I could use on human FFPE samples that shows relative degrees of reactivity, based on variable factors such as fixation time or tissue autolysis? I would like to assess the reactivity of commercially available tissue bank samples. Any suggestions would be appreciated. Tom Crowell Biogen Idec Cambridge, MA ------------------------------ Message: 7 Date: Wed, 22 Mar 2006 14:00:54 -0600 From: "Sebree Linda A." Subject: RE: [Histonet] Fixation sensitive IHC markers To: "Thomas Crowell" , Message-ID: Content-Type: text/plain; charset="US-ASCII" Our pathologists have relied on a vimentin stain to assess the quality of fixation and processing. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas Crowell Sent: Wednesday, March 22, 2006 1:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fixation sensitive IHC markers Dear Histonetters, Can anyone suggest an antibody that I could use on human FFPE samples that shows relative degrees of reactivity, based on variable factors such as fixation time or tissue autolysis? I would like to assess the reactivity of commercially available tissue bank samples. Any suggestions would be appreciated. Tom Crowell Biogen Idec Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 8 Date: Wed, 22 Mar 2006 14:10:49 -0600 From: "Sebree Linda A." Subject: RE: [Histonet] CAP - ANP.11713 Phase II To: "Rebecca Barnhart" , Message-ID: Content-Type: text/plain; charset="US-ASCII" In our lab (IHC & ISH), every case that goes out to the pathologist is accompanied by the original request form that has a "QA" form on the back. The pathologist is obligated to select "satisfactory" or "unsatisfactory" in response to the statement: "I have reviewed the patient and positive/negative control slides associated with the immunostains requested on this case and find them satisfactory/unsatisfactory" and initial his/her response. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rebecca Barnhart Sent: Wednesday, March 22, 2006 11:44 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] CAP - ANP.11713 Phase II I created a database that we enter our daily count (the date, first and last case number, the number of blocks and slides). Also there are 2 prompts to be checked by the pathologist, one for H & E and the other for cytology stain. We run a control as the first and last slide everyday. If the stain is ok he puts a check in both boxes, if there is a problem there is a spot to enter comments. I started doing it this way so that if there is a problem with the stain I can easily go back and see what cases it affected. I have a report that I can print to show the quality of stain if an inspector request it. Previously we had a sheet the pathologist kept at his desk and check each day if the stain was ok and make comments if not. I also use the spot for comments to document if we have changed something (trying a different stain, new paraffin, added time to a step on the processor or stainer, etc). So if there are questions or we need to compare slides (because of a new product or new procedure) I have a report that I run and I can easily pull the slides. I also use this database to enter all statistical information. So at any time I can give the pathologist or manager any number for anatomic pathology. >>> "Demarinis, Carolyn" 3/22/2006 11:34:14 AM >>> There is a new question on the CAP checklist: ANP.11713 - Phase II "Is there documented evidence of daily review of technical quality of histologic preparations by the pathologist?" Our present procedure is the supervisor/technologist documents daily review, but it is very clear that CAP is requiring the pathologist to initial a document daily proving they have reviewed the technical quality of slides. How are other hospitals satisfying this requirement? It seems crazy to make a pathologist initial a document daily, when it is evident they review hundreds of slides a day, and they would certainly report a problem to the supervisor when the stain is unacceptable. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Wed, 22 Mar 2006 15:41:08 -0500 From: "Andrea T. Hooper" Subject: Re: [Histonet] DAB in tablet form To: Histonet , meint002 Message-ID: Content-Type: text/plain; charset=iso-8859-1; format=flowed I too am in academic labs so relate to the cost concern; however, I strongly recommend DAB in liquid kit form - either DAB+ from DAKO or from Zymed. They are liquid ready to use concentrates diluted into buffer either supplied with the kit or you provide yourself. I have used both for years and years now with excellent results. Worth the extra bucks (about 80-100 dollars for 110 mls which is actually quite a lot) for consistent results time after time after time - without the hazards of working with powder or solids. Good luck! Andrea >Dear Histos, > >Due to the hazards of working with 3,3? diaminobenzidine tetrahydrochloride >(DAB) for muscle enzyme staining, I am considering purchasing DAB as either >5mg, or 10mg tablets. Some companies have them dissolve in water and others >in a buffer solution. > >I am looking for input from anyone who has used these tablets. Do you like >them, do they work well, staining results, which company, etc.... I know >that you can also get DAB in sealed separate vials, but it is much more >expensive and we are in an academic research setting where cost is a >consideration. > >I am particularly interested in using them in the Cytochrome Oxidase stain. >I have tried many procedures for this stain and my Dr. is not all that >pleased with the results. If anyone has a good procedure for this stain, I >would be grateful if you would send me a copy. > >I am revamping this lab and have had a lot of questions for the histonet that >you have been very helpful in addressing. > >Thanks for all the suggestions in advance. > > >Joyce Meints >Histologist > >University of Minnesota >Paul and Sheila Wellstone Muscular Dystrophy Center >MMC 206 >420 Washington Ave. SE >Minneapolis, MN 55455-0392 > >e-mail: meint002@umn.edu >Fax: 612-625-8488 >lab phone: 612-626-4703 -- ------------------------------ Message: 10 Date: Wed, 22 Mar 2006 12:45:12 -0800 From: Patti Loykasek Subject: Re: [Histonet] guidelines for Her2 interpretation To: "Goodwin, Diana" , histonet Message-ID: Content-Type: text/plain; charset="US-ASCII" Diana, yes the standard is the Herceptest guideline, as far as I know. I haven't looked at some of the more recent FDA approved tests. I highly recommend correlating your scores with Her2 FISH results. Also, if you have >1 pathologist, the pathologists should periodically review cases together so that their eyes stay "calibrated" to the same level of staining/scoring. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA I have been out of the loop with Her- 2 for a while, and the last I > knew, it was left up to the individual labs to establish standards among > the interpreting physicians for interpretation, pretty much based on > the original Herceptest guidelines. Have any other standards been > established? If so, would someone please provide me with a reference? > > Much obliged, > Diana Goodwin > Supervisor, Anatomic Pathology > Pennsylvania Hospital > Preston 655-C > ph. 215-829-6532 > fax 215-829-7564 > e-mail goodwind@[pahosp.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. ------------------------------ Message: 11 Date: Wed, 22 Mar 2006 14:03:34 -0600 From: "Dick Paulson [Source Medical Products]" Subject: [Histonet] p16 antibody To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Dr. Cartun, Diagnostic BioSystems can provide the p16 antibody. Clone(JC8) Isotype(IgG2a) If you have any questions, please contact me directly. Source Medical Products Dick Paulson dpconsult@earthlink.net Phone (800) 393-6345 ---- Original Message ---- We have been using Novocastra's p16 antibody (distributed by Vision BioSystems here in the USA). I have been told that their antibody is back-ordered. I know DAKO sells a p16 kit, but I'm only interested in getting the antibody. Are there any other sources for p16 that work in formalin-fixed, paraffin-embedded tissues? Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax ------------------------------ Message: 12 Date: Wed, 22 Mar 2006 14:58:31 -0600 From: bamoe@gundluth.org Subject: [Histonet] Contaminated Alcohols from VIP processors To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Hello all - Has anyone had experience with contaminated alcohols coming off a Tissue-Tek VIP processor? We have two processors - one is a Miles VIP 3000, purchased in 1993, the other is a Sakura VIP E300 purchased in 1996. We have started experiencing the problem on both machines. Our processing schedule utilizes 2 stations each of formalin, 80% ETOH, recycled 95% ETOH, absolute ETOH (pure, not reagent grade), xylene, 3 or 4 paraffin stations, 1 cleaning xylene, 1 cleaning absolute ETOH. The formalins and 80% are not contaminated, however the 95% and absolutes show cloudiness when flushed with water down the drain. (We are assuming that it's xylene causing this, but have not done analysis to confirm.) Preventative maintenance was just done last week on both machines (by our in-house biomed personnel) and we still have the cloudiness in the 95% and absolute alcohols. We have ruled out any problems with our recycler and know that the alcohols are clean when they are put on the processor. Any comments would be greatly appreciated! Barb Moe Gundersen Lutheran Medical Center La Crosse WI bamoe@gundluth.org ------------------------------ Message: 13 Date: Wed, 22 Mar 2006 14:27:28 -0700 From: Gayle Callis Subject: Avoiding Re: [Histonet] DAB in tablet form To: "Andrea T. Hooper" , Histonet@lists.utsouthwestern.edu Message-ID: <6.0.0.22.1.20060322142207.01b3db88@gemini.msu.montana.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed I totally agree with Andrea. Another superb two part DAB substrate kit is Pierce DAB (brown) $65 for 250 ml kit or Pierce CN/DAB (black) $78 for 250 ml kit, and you can make up as much or little as you want for number of slides in a run. Acadamic labs often get discounted pricing and free shipping other than hazardous material costs, this can be purchased through Fisher and probably VWR too. At 01:41 PM 3/22/2006, you wrote: >I too am in academic labs so relate to the cost concern; however, I >strongly recommend DAB in liquid kit form - either DAB+ from DAKO or from >Zymed. They are liquid ready to use concentrates diluted into buffer >either supplied with the kit or you provide yourself. > >I have used both for years and years now with excellent results. Worth the >extra bucks (about 80-100 dollars for 110 mls which is actually quite a >lot) for consistent results time after time after time - without the >hazards of working with powder or solids. > >Good luck! >Andrea Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) ------------------------------ Message: 14 Date: Wed, 22 Mar 2006 13:30:00 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] DAB in tablet form To: meint002 , histonet@lists.utsouthwestern.edu Message-ID: <20060322213000.38655.qmail@web61221.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Joyce: I used for years the tablets with excellent results. I prefer the tablets and to dissolve them when needed. Ren? J. meint002 wrote: Dear Histos, Due to the hazards of working with 3,3' diaminobenzidine tetrahydrochloride (DAB) for muscle enzyme staining, I am considering purchasing DAB as either 5mg, or 10mg tablets. Some companies have them dissolve in water and others in a buffer solution. I am looking for input from anyone who has used these tablets. Do you like them, do they work well, staining results, which company, etc.... I know that you can also get DAB in sealed separate vials, but it is much more expensive and we are in an academic research setting where cost is a consideration. I am particularly interested in using them in the Cytochrome Oxidase stain. I have tried many procedures for this stain and my Dr. is not all that pleased with the results. If anyone has a good procedure for this stain, I would be grateful if you would send me a copy. I am revamping this lab and have had a lot of questions for the histonet that you have been very helpful in addressing. Thanks for all the suggestions in advance. Joyce Meints Histologist University of Minnesota Paul and Sheila Wellstone Muscular Dystrophy Center MMC 206 420 Washington Ave. SE Minneapolis, MN 55455-0392 e-mail: meint002@umn.edu Fax: 612-625-8488 lab phone: 612-626-4703 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. PC-to-Phone calls for ridiculously low rates. ------------------------------ Message: 15 Date: Wed, 22 Mar 2006 15:33:19 -0600 From: "Charles.Embrey" Subject: RE: [Histonet] Contaminated Alcohols from VIP processors To: Cc: Histonet@pathology.swmed.edu Message-ID: Content-Type: text/plain; charset="us-ascii" I had the same problem and worked with my sales rep. I was finally told that what I was reporting was impossible. When it was verified by equipment repair, my rep told me that the bottom line was that Sakura sold me a tissue processor and it did process tissue. They made no promise that I would be able to recycle the reagents so the matter was closed. Needless to say, I replaced the VIP with a TBS unit and now have no problem recycling my alcohol. Charles Embrey PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of bamoe@gundluth.org Sent: Wednesday, March 22, 2006 2:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Contaminated Alcohols from VIP processors Hello all - Has anyone had experience with contaminated alcohols coming off a Tissue-Tek VIP processor? We have two processors - one is a Miles VIP 3000, purchased in 1993, the other is a Sakura VIP E300 purchased in 1996. We have started experiencing the problem on both machines. Our processing schedule utilizes 2 stations each of formalin, 80% ETOH, recycled 95% ETOH, absolute ETOH (pure, not reagent grade), xylene, 3 or 4 paraffin stations, 1 cleaning xylene, 1 cleaning absolute ETOH. The formalins and 80% are not contaminated, however the 95% and absolutes show cloudiness when flushed with water down the drain. (We are assuming that it's xylene causing this, but have not done analysis to confirm.) Preventative maintenance was just done last week on both machines (by our in-house biomed personnel) and we still have the cloudiness in the 95% and absolute alcohols. We have ruled out any problems with our recycler and know that the alcohols are clean when they are put on the processor. Any comments would be greatly appreciated! Barb Moe Gundersen Lutheran Medical Center La Crosse WI bamoe@gundluth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Wed, 22 Mar 2006 17:08:34 -0500 From: Jeff Silverman Subject: [Histonet] CAP Standard ANP.11713 - Phase II To: cdemarinis@SARATOGACARE.ORG Cc: histonet@lists.utsouthwestern.edu Message-ID: <000001c64dfd$297e3740$6401a8c0@jeffrey028c8d9> Content-Type: text/plain; charset=us-ascii Previously, either myself of the lead tech for the day would examine a control slide- a tonsil or fallopian tube from the day's workload, to document proper staining and processing. To comply with this standard during our December survey, we created a daily form (yet another) distributed to whichever of our three pathologists is signing out that day. Any deficiencies or problems in a specific slide including staining issues, folds, wrinkles, or scratches, incomplete sections, misoriented tissues, floaters or contaminants, bubbles under the coverslip, mislabeling are listed by the docs. The histotech involved must address the problem and document what was done to remedy the situation. This provides a tool for continuing quality improvement so trends can be identified and individuals with chronic problems can be retrained/counseled. Jeffrey Silverman HT HTL QIHC (ASCP) Pathologists' Assistant Southside Hospital Bay Shore New York USA ------------------------------ Message: 17 Date: Wed, 22 Mar 2006 18:02:36 -0500 From: Luis Chiriboga Subject: RE: [Histonet] Fixation sensitive IHC markers To: Thomas Crowell , histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii you might want to take a look at: "Assessment of antigen damage in immunohistochemistry. the Vimentin internal control" by Hector Battifora. American Journal of Clinical Pathology November 1991 96(5) 669. Hope it helps Luis ____________________________________ Luis Chiriboga Ph.D. NYU Cancer Institute and Bellevue Hospital Center New York University School Of Medicine Department Of Pathology 4W27 462 First Avenue New York, N.Y. 10016 W(212) 562-4667. F(212) 263-2041 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Thomas Crowell Sent: Wednesday, March 22, 2006 2:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fixation sensitive IHC markers Dear Histonetters, Can anyone suggest an antibody that I could use on human FFPE samples that shows relative degrees of reactivity, based on variable factors such as fixation time or tissue autolysis? I would like to assess the reactivity of commercially available tissue bank samples. Any suggestions would be appreciated. Tom Crowell Biogen Idec Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Wed, 22 Mar 2006 18:20:21 -0500 From: "Monfils, Paul" Subject: RE: [Histonet] Contaminated Alcohols from VIP processors To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <09C945920A6B654199F7A58A1D7D1FDE017176AA@lsexch.lsmaster.lifespan.org> Content-Type: text/plain; charset="iso-8859-1" I have noticed the same thing, and I don't think the cloudiness is due to xylene. I believe it is due to water-insoluble substances, particularly lipids, which have leached out of the tissue samples into the stronger alcohols. Because these substances are water-insoluble they don't tend to dissolve in the formalin or the weaker alcohols where a lot of water is present, but they do dissolve somewhat in the stronger alcohols. Also, because they are water-insoluble, they precipitate (become cloudy) when water is added to the alcohol, as in flushing down the drain. Because I'm in a service research lab, I often run one kind of tissue per processing run, and sometimes only one kind of tissue for several days. I have noticed that the degree of cloudiness relates not only to the number of specimens I have run since the last solvent change, but also very much to the kind of tissues that were processed. I can run kidney or lung or muscle specimens all week and there is minimal cloudiness when I discard the alcohols. But if I process a large number of skin or breast or colon or other fatty samples, the alcohol turns milky white when it hits the water. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > bamoe@gundluth.org > Sent: Wednesday, March 22, 2006 12:58 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Contaminated Alcohols from VIP processors > > > > > > Hello all - > > Has anyone had experience with contaminated alcohols coming off a > Tissue-Tek VIP processor? > > We have two processors - one is a Miles VIP 3000, purchased in 1993, the > other is a Sakura VIP E300 purchased in 1996. > We have started experiencing the problem on both machines. > > Our processing schedule utilizes 2 stations each of formalin, 80% ETOH, > recycled 95% ETOH, absolute ETOH (pure, not reagent grade), xylene, 3 or 4 > paraffin stations, 1 cleaning xylene, 1 cleaning absolute ETOH. > > The formalins and 80% are not contaminated, however the 95% and absolutes > show cloudiness when flushed with water down the drain. (We are assuming > that it's xylene causing this, but have not done analysis to confirm.) > > Preventative maintenance was just done last week on both machines (by our > in-house biomed personnel) and we still have the cloudiness in the 95% and > absolute alcohols. > > We have ruled out any problems with our recycler and know that the > alcohols > are clean when they are put on the processor. > > Any comments would be greatly appreciated! > > Barb Moe > Gundersen Lutheran Medical Center > La Crosse WI > bamoe@gundluth.org > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 19 Date: Wed, 22 Mar 2006 18:25:57 -0500 From: "Nellis, Kevin Lee \(NIH/NCI\) [E]" Subject: [Histonet] National Search for Histology Supervisor at the National Cancer Institute; up to $84,559 per year To: Message-ID: Content-Type: text/plain; charset="us-ascii" Dear Collegues: The NCI Laboratory of Pathology is are actively recruting for a Histology Supervisor position. The job announcement is available on the www.USAJobs.gov website. The position number is NCI-06-116352. You may review the full details at: http://jobsearch.usajobs.opm.gov/getjob.asp?JobID=41029650 There are three different government salary ranges for this position based on the level of experience of the candidate. Please see the full positing for details for examples of specialized experience. If a GS-10 level candidate is selected, the selected individual will be placed in a Career Development Program. A successful candidate can be promoted to the full level of a GS-12, during his/her career. The salary ranges including locality pay are: GS-10, Salary range $ 49,397 - $ 64,213 GS-11, Salary range $ 54,272 - $ 70,558 GS-12, Salary range $ 65,048 - $ 84,559 RELOCATION EXPENSES AND RECRUITMENT BONUS MAY BE PAID. Interested parties may apply online at on the www.USAJobs.gov website or you send an application directly to Ernesto Corrales. Be sure to read the announcement carefully and supply all the information. Applicants MUST submit a supplemental statement to their resume or application addressing each of the specific KSAs. Only the most highly qualified candidates will be referred to the hiring manager. The application deadline is Tuesday, April 11, 2006. For information about the NIH and surrounding areas, please see www.nih.gov For information about benefits and awards please see: http://hr.od.nih.gov/default.htm Thank you for your interest. Please forward this e-mail to anyone that might consider this exciting opportunity. Kevin L. Nellis, M.S., M.T. (A.S.C.P.) Clinical Laboratory Manager Laboratory of Pathology Center for Cancer Research National Cancer Institute National Institutes of Health U.S. Department of Health and Human Services 10 Center Drive, Room 2A33, MSC 1500 Bethesda, MD 20892 OFFICE: (301) 594-9532 FAX: (301) 402-0043 http://home.ncifcrf.gov/ccr/lop/Clinical/default.asp ------------------------------ Message: 20 Date: Wed, 22 Mar 2006 22:12:57 -0800 From: Jennifer MacDonald Subject: Re: [Histonet] Training Med Techs - some candid comments To: "Mike Kirby" Cc: "Histonet \(E-mail\)" Message-ID: Content-Type: text/plain; charset="ISO-8859-1" Dear Mr. Kirby, I do not usually get involved in the mu but your response has changed that. Your Becky and to all Histotechs. Becky was not ins interpretation is that she is frustrated with Hi overlooked for management positions in favor of Med Techs.&n train a Med Tech with no histology experience if you have a very c apable Histotech available? She did not mean to imply that Histotechn meant that o train somebody on t correct me if I am wrong. As a Med Tech, I have worked in all areas of those fancy biochemistry and hematology analyzers, as matching blood for an ER patient. I know what stress i work in histology where things can also go wrong and depend the laboratory the turn-around-time expectations can be unreasonable . What we do in Histology can also impact a patient's health. A lthough the Pathologist makes the diagnosis, it is made on slides that we h grievous pr your ability hands throughout < gruelin Techs. Many h There are many of us wh considered a boring "dead end division" field because we truly enjoy wh the respect we get from people like Jennifer MacDonald -----histonet-bounces@lists.utsouthwestern.edu wrote To: "Histonet \(E-mail\)" Fro Sent by: histonet-bounces@ Date: 03/22/2006 05:57AM Subject: [Histonet] Dear Ms Orr. You ma one! Cli Your comment " Training a MT to be train a policeman to be a fireman" rather s I would like to follow with a counter comment, "C can be shown how to the flames. Granted, His requiring fine manual dext better than operating a high output Bi analyser or cross-matching a pint of blood, where a can kill a patient. Plus you operate under a fraction of the stress we are subjected to - try working for a full day, and then doi expected to report f It's a gross insult to insinuate that ours is a "career". As students, we spent 5 - 6 mo was available in the lab - Chempath, Ha Cyto, Immunology, Human genetics, blood transfusio even Histopath. When we passed our finals, we were allowed to c hoose which discipline we wanted to further our careers, and each year, wit Histopath, as it was cons (Yes, pun intended). As fo Dept, then you c systems apply, regardless o practical applications of the work in hand Histopathology is just one of the services in the medi you don't walk on water, and neither do we. As far as I am conce rned, once trained as a general Med Tech, you can be trained in virtually a thumbs" me, who after 35 half decent section or make a passa good as a manager!) End of tirade - climb into bunker, to await the verbal unleashed................ Mr.M.Kirby J South Africa -----Original Message----- Fr [[1]mailto:histonet-bounces@lists.utsou Of Orr, Rebecca Sent: 17 March 2006 17:04 To Cc: &n Subject: [Histonet] Training Med Techs H I would appreciate any feedback from those of you who may to train MT's (ASCP) to work in Histology. They would be train them into Anatomic Patho Candid comments welcome, especial histology! To me it would be like trying to fireman, it's a career, not a job, right? We see a HT shortage in the Chicago area, but I am unsure how to addre Degreed individuals have proven critical thinkin traditional education pathway, so I see the advantages, but ignore very capable HT managers with proven management and organizationa an issue with me.I mean it's not like Non degreed HT's are stooopid or something. < Becky Becky Orr CLA,HT(ASCP) IHC Lead Eva 847-570-2771 ____ ______________________ 5F__ Histonet mailing Histonet@lists.utsouthwestern.edu [2]http://lists.utsou ************************ ********************************************************** The views exp those of the author and Laboratory Services or its management. The e-mail is confidential and is intended solely for the Access to this e-mail by anyone else is unauthorized. If you not the intended recipient, any disclosure, copying, distribution or an and may be unl Whilst all reasonable steps are taken to ensure the accuracy and integrity of information and data transmitted electronically and to preserv responsibility whatsoever is for whatever reason, corrupted or does destination. ******************************* **************************************************** _ ______________________ 5F__ _____________________ Histonet Histonet@lists.utsouthwestern.edu [3]http://li References 1. 3D"mailto:histonet-bounces@li 2. 3D"http://lists.utsout=/ 3. 3D"http://lis=/ ------------------------------ Message: 21 Date: Thu, 23 Mar 2006 05:34:29 -0700 From: "Jesus Ellin" Subject: [Histonet] MAK 6 HELP PLEASE!!!!!!!!!!! To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Hello everyone I need some really big help we are trying to add an additional antibody to our system MAK6 from Zymed. We are using Ventana stainers, one is a Benchmark the other is LT. If anyone out there is using this antibody on these stainers and having success, PLEASE!!!!!!!!!!!! let me have your protocol for pariffin embeded and formalin fixed tissue. Jesus Ellin Yuma Regional Medical Center ------------------------------ Message: 22 Date: Thu, 23 Mar 2006 15:00:04 -0000 From: "MattG" Subject: [Histonet] Fw: Thermo/ Leica To: Message-ID: <001501c64e8a$785f4240$6501a8c0@acer311vpbceh0> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=response Does anyone have a current email address for Pauline Fisher of Thermo (formerly Shandon)? I'd also be grateful if someone could send me an electronic version of the user manual for the Leica TP1050. If there are any UK based Leica or Thermo reps on here, could one of you please drop me an email as I have a couple of questions I'd like to ask. Thanks, Matt Griffiths ___________________________________________________________ Win a BlackBerry device from O2 with Yahoo!. Enter now. http://www.yahoo.co.uk/blackberry ------------------------------ Message: 23 Date: Thu, 23 Mar 2006 10:34:15 -0500 From: "Bonner, Janet" Subject: RE: [Histonet] Contaminated Alcohols from VIP processors To: bamoe@gundluth.org, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=iso-8859-1 I experienced this problem before and discovered the 95% ETOH was the culprit ( the 100%s could be white due to the 95% carried over into them). The company is going to deny this of course, but our supplier later came back to say there was a manufacturing problem that was not picked up on in their QC. Try another brand before you do anything, or call your supplier to ask if there are any other reported problems. -Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of bamoe@gundluth.org Sent: Wed 3/22/2006 3:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Contaminated Alcohols from VIP processors Hello all - Has anyone had experience with contaminated alcohols coming off a Tissue-Tek VIP processor? We have two processors - one is a Miles VIP 3000, purchased in 1993, the other is a Sakura VIP E300 purchased in 1996. We have started experiencing the problem on both machines. Our processing schedule utilizes 2 stations each of formalin, 80% ETOH, recycled 95% ETOH, absolute ETOH (pure, not reagent grade), xylene, 3 or 4 paraffin stations, 1 cleaning xylene, 1 cleaning absolute ETOH. The formalins and 80% are not contaminated, however the 95% and absolutes show cloudiness when flushed with water down the drain. (We are assuming that it's xylene causing this, but have not done analysis to confirm.) Preventative maintenance was just done last week on both machines (by our in-house biomed personnel) and we still have the cloudiness in the 95% and absolute alcohols. We have ruled out any problems with our recycler and know that the alcohols are clean when they are put on the processor. Any comments would be greatly appreciated! Barb Moe Gundersen Lutheran Medical Center La Crosse WI bamoe@gundluth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 24 Date: Thu, 23 Mar 2006 16:11:00 GMT From: "mprice26@juno.com" Subject: [Histonet] RE:JOB DESCRIPTION FOR HISTOLOGY ASSISTANT To: HISTONET@LISTS.UTSOUTHWESTERN.EDU Message-ID: <20060323.081133.783.179@webmail01.nyc.untd.com> Content-Type: text/plain HI HISTONETTERS, Can someone be so kind as to share a job description with me for a histology dept.assistant/aide (Non Registered). Thank you. Marsha Price ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 28, Issue 37 **************************************** CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From vazquezr <@t> ohsu.edu Mon Mar 27 09:07:03 2006 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Mon Mar 27 09:07:27 2006 Subject: [Histonet] colored OC T media - not TBS Message-ID: Maria, We use the same OCT and add food coloring to it...works perfect. Make it in advanced and put it on a rotator after you shake it up. And you can experiment with different colors. Hope that helps... Robyn OHSU >>> "Maria Mejia" 3/23/2006 7:46 AM >>> Does anyone know where I can buy "colored" OCT freezing media that is NOT TBS media? Many thanks. Maria Bartola Mejia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mgdelaware <@t> comcast.net Mon Mar 27 11:46:12 2006 From: mgdelaware <@t> comcast.net (Marian powers) Date: Mon Mar 27 11:45:19 2006 Subject: [Histonet] Mini auto stainers Message-ID: <000c01c651c6$56ab7710$be4dff45@MEGAN> Looking for a mini auto stainer other than Thermo's linistat. Thanks in advance. Marian From Jerry <@t> ralambusa.com Mon Mar 27 12:13:00 2006 From: Jerry <@t> ralambusa.com (Jerry Helisek) Date: Mon Mar 27 12:14:24 2006 Subject: [Histonet] Embedding Plant Tissue (Mainly, Corn & Soybean) Message-ID: <3855F92002259948A66A8CA2D16E3A4F457B@server.ralambusa.com> What would the best "wax" to use for embedding Plant tissue... mainly Corn & Soybean. Thank Jerry Helisek From fawn <@t> cs.cmu.edu Mon Mar 27 13:05:56 2006 From: fawn <@t> cs.cmu.edu (Fawn Jones) Date: Mon Mar 27 13:05:58 2006 Subject: [Histonet] T Blue staining procedure Message-ID: <44283794.10301@cs.cmu.edu> Hi histonetters, I was wondering if anyone happens to have a good Toluidine Blue staining procedure for MMA embedded slides. Any help is greatly appreciated. Fawn Jones From jtrynak <@t> hotmail.com Mon Mar 27 13:09:56 2006 From: jtrynak <@t> hotmail.com (John Rynak) Date: Mon Mar 27 13:10:01 2006 Subject: [Histonet] Wanted: Application Specialist - IHC TX or TN Message-ID: Application Specialist - IHC SciGenium's Client is experiencing tremendous growth within their North American operations. This growth is due to the recent launch of their FDA approved automated histology instrumentation system, and the growing antibody and reagent consumable product line for the histology market. Due to the new launch of these products they are currently looking for several Application Specialist - IHC, in the following regions : Southwest Region (TX Houston or Dallas ) and TN Position provides technical applications support for our next generation range of automated histopathology products. This is a very hands on role where your key tasks will include: · Building an in-depth understanding of our new product technologies and their applications in a diagnostic histopathology environment; · Customer liaison by providing scientific and laboratory expertise for in-field installations, training and trouble-shooting; · Customer support Help-line for remote problem solving; · Designing and performing experiments to investigate and solve tough technical applications problems; and · Preparing problem resolution reports and imparting your findings and knowledge to internal and field based Product Engineers and Technical Managers. To be successful in this role you will possess: · Excellent problem solving and analytical skills · The ability to communicate with front-end users, technical design engineers and pragmatic sales force members. · A tertiary qualification in a relevant science stream (eg. Medical Laboratory Science) · Practical experience in bench-level histopathology, including clinical immunohistochemistry · Demonstrated use of automated scientific instrumentation BS/ MS in Life Sciences and ASCP Certification Interest in using PC based software and maintenance of automated scientific instrumentation will be highly advantageous. In addition, you are extremely detailed and analytically minded with a strong sense of customer focus. Interested parties should forward a resume to resume@scigenium.com Send to : SciGenium PO 380916 Cambridge, MA 02238 From LuckG <@t> empirehealth.org Mon Mar 27 12:34:01 2006 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Mon Mar 27 13:21:19 2006 Subject: [Histonet] PAX-2 Ab. Message-ID: Hello, Could someone supply me with the/a vendor for an antibody called "PAX-2" for FFPE. Thanks, Greg Greg Luck Anatomic Pathology Supervisor Deaconess Med Center / EHS 800 W. 5th Ave Spokane, WA 99204 Phone 509.473.7394 Fax 509.473.7133 luckg@empirehealth.org From Jeanne.Reis <@t> biogenidec.com Mon Mar 27 14:00:06 2006 From: Jeanne.Reis <@t> biogenidec.com (Jeanne Reis) Date: Mon Mar 27 14:00:14 2006 Subject: [Histonet] Re: Histonet Digest, Vol 28, Issue 44 In-Reply-To: Message-ID: Robyn, Try Thermo for frozen embedding matrix. I've used for years. Jeanne From gcallis <@t> montana.edu Mon Mar 27 14:18:43 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Mar 27 14:19:27 2006 Subject: [Histonet] T Blue staining procedure In-Reply-To: <44283794.10301@cs.cmu.edu> References: <44283794.10301@cs.cmu.edu> Message-ID: <6.0.0.22.1.20060327131744.01b4e720@gemini.msu.montana.edu> The best one I have used was developed by Diane Sterchi, and is a publication in J of Histotechnology. I will attach it privately. At 12:05 PM 3/27/2006, you wrote: >Hi histonetters, > >I was wondering if anyone happens to have a good Toluidine Blue staining >procedure for MMA embedded slides. Any help is greatly appreciated. > >Fawn Jones > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From Margaret.Perry <@t> sdstate.edu Mon Mar 27 17:01:11 2006 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Mon Mar 27 17:01:17 2006 Subject: [Histonet] IHC testing Message-ID: I have used the HistoQip program and learned a lot. I'm now interested in finding a program like it for IHC. Does anyone out there in histoland know where I could find such a program if one exists? Margaret Perry HT(ASCP) IHC Lab Manager Veterinary Science South Dakota State University From failm <@t> musc.edu Mon Mar 27 17:14:18 2006 From: failm <@t> musc.edu (Mildred Fail) Date: Mon Mar 27 17:17:59 2006 Subject: [Histonet] IHC testing Message-ID: CAP Rena Fail >>> "Perry, Margaret" 03/27/06 18:04 PM >>> I have used the HistoQip program and learned a lot. I'm now interested in finding a program like it for IHC. Does anyone out there in histoland know where I could find such a program if one exists? Margaret Perry HT(ASCP) IHC Lab Manager Veterinary Science South Dakota State University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From failm <@t> musc.edu Mon Mar 27 17:16:00 2006 From: failm <@t> musc.edu (Mildred Fail) Date: Mon Mar 27 17:19:39 2006 Subject: [Histonet] IHC testing Message-ID: CAP offers a similar program for IHC twice a year Rena Fail Rena Fail >>> "Perry, Margaret" 03/27/06 18:04 PM >>> I have used the HistoQip program and learned a lot. I'm now interested in finding a program like it for IHC. Does anyone out there in histoland know where I could find such a program if one exists? Margaret Perry HT(ASCP) IHC Lab Manager Veterinary Science South Dakota State University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From amosbrooks <@t> gmail.com Mon Mar 27 18:22:24 2006 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Mon Mar 27 18:22:28 2006 Subject: [Histonet] Tissue Request: Rapid Processor Message-ID: <582736990603271622k5a477428i8bef1871c9558343@mail.gmail.com> Hi, I would like to ask if there is anyone that happens to be using the Sakura Rapid Tissue Processor that would be willing to send a sample of tissue to me. Our lab is getting one and I would like to try working with some tissue that has been processed in this manner in order to try to get a head start on optimizing cutting and testing and such. I am ideally interested in tonsil or lymph node and colon, but I would not be adverse to other types as well. Let me know if you would be willing to share. Thanks, Amos Brooks From cmalc <@t> unimelb.edu.au Mon Mar 27 19:34:18 2006 From: cmalc <@t> unimelb.edu.au (Cathy Malcontenti-Wilson) Date: Mon Mar 27 18:34:38 2006 Subject: [Histonet] Eosin Y alcoholic with or without phloxine? Message-ID: <6.2.1.2.2.20060328111844.02dcf5e0@mail.staff.unimelb.edu.au> Dear All, I would like to ask about a particular problem with eosin-Y staining. I am using Eosin Y alcoholic without phloxine (from Sigma) and for some reason, all of the eosin staining comes out of the tissue as it is being dehydrated. I can actually see that it just drains out. I know that this is part of the differentiation process but it just all comes out. We use the same staining set for mouse liver tumour tissues with no problem. These are routinely processed (by a hospital pathology lab) rat rectum slides. Is the phloxine important for "keeping in the stain"? The sections are quite small. Should I be using a different eosin? Any advice on this matter would be greatly appreciated. Cathy From jnocito <@t> satx.rr.com Mon Mar 27 20:23:07 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Mar 27 20:23:08 2006 Subject: [Histonet] Venting Part II Message-ID: <00c001c66a62$4aa4a040$e8bd0b43@yourxhtr8hvc4p> Okay, as I said in my apology posting, I used "dumb ass" to describe the plumber who did not want to vent the flame cabinets. Please give me an alternative phrase for me to use because I don't understand the following events: 1. what do you call it when you put in a drench shower/eyewash station combo, but don't put in a drain? 2. what do you call it when you leave a 2" pipe open near the bottom of the shower/eyewash so that when you test the eyewash station, a rush of water soaks your shoes? 3. what do you call it when you put in a deionized water station, but don't know what a conductivity light is? So when you ask the plumber "how do you when to change the tank if there is no light", and the plumber just says "I don't know"?. 4. what do you call it when you blow a test ball through the drain system to test the drain system, yet forget to take the test ball out of the drain and water backs up into your new lab causing a huge flood in the lab? Then have to call in a competitor to help you fix it. 5. what do you call it when you try to light a gas water heater for 30 minutes, just to be told by some one else that there is no gas meter hooked up because you forgot to get the meter from the city? 6. what do you call it when you are soldering a pipe and activate the fire alarm system? I fell like Jeff Foxworthy "You might be a redneck if". Can some one please come up with an alternative phrase other than "dumb ass"? These events really happened this weekend in my new $1.5 million lab. I know, it sounds like fiction, but trust me, it's the real thing. So you see my dilemma. And for that one person who I offended in the past, I may be unprofessional in my language and conduct, but I do know MY job. Thanks for listening. In advance, I am sorry for the harsh language once again, but I can't come up with the words to accurately describe this "professional" plumber. Let the flaming begin (I'll just have to get another plumber to help put out the flames) Joe From Jackie.O'Connor <@t> abbott.com Tue Mar 28 07:05:11 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Tue Mar 28 07:05:36 2006 Subject: [Histonet] Venting Part II Message-ID: I think you call that a Union Man. "Joe Nocito" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/27/2006 08:23 PM To: "histonet" cc: (bcc: Jackie M O'Connor/LAKE/GPRD/ABBOTT) Subject: [Histonet] Venting Part II Okay, as I said in my apology posting, I used "dumb ass" to describe the plumber who did not want to vent the flame cabinets. Please give me an alternative phrase for me to use because I don't understand the following events: 1. what do you call it when you put in a drench shower/eyewash station combo, but don't put in a drain? 2. what do you call it when you leave a 2" pipe open near the bottom of the shower/eyewash so that when you test the eyewash station, a rush of water soaks your shoes? 3. what do you call it when you put in a deionized water station, but don't know what a conductivity light is? So when you ask the plumber "how do you when to change the tank if there is no light", and the plumber just says "I don't know"?. 4. what do you call it when you blow a test ball through the drain system to test the drain system, yet forget to take the test ball out of the drain and water backs up into your new lab causing a huge flood in the lab? Then have to call in a competitor to help you fix it. 5. what do you call it when you try to light a gas water heater for 30 minutes, just to be told by some one else that there is no gas meter hooked up because you forgot to get the meter from the city? 6. what do you call it when you are soldering a pipe and activate the fire alarm system? I fell like Jeff Foxworthy "You might be a redneck if". Can some one please come up with an alternative phrase other than "dumb ass"? These events really happened this weekend in my new $1.5 million lab. I know, it sounds like fiction, but trust me, it's the real thing. So you see my dilemma. And for that one person who I offended in the past, I may be unprofessional in my language and conduct, but I do know MY job. Thanks for listening. In advance, I am sorry for the harsh language once again, but I can't come up with the words to accurately describe this "professional" plumber. Let the flaming begin (I'll just have to get another plumber to help put out the flames) Joe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> u.arizona.edu Tue Mar 28 07:39:02 2006 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Tue Mar 28 07:39:09 2006 Subject: [Histonet] Embedding Plant Tissue (Mainly, Corn & Soybean) In-Reply-To: <3855F92002259948A66A8CA2D16E3A4F457B@server.ralambusa.com> Message-ID: <4.3.2.7.2.20060328063217.024c49a8@algranth.inbox.email.arizona.edu> Jerry, I use regular Paraplast or I have used Richard Allan Type 6 to embed leaves and stems from plants like alfalfa and even cactus spines. For seeds you might want to use a harder paraffin. I checked in Ruzin's, Plant Microtechnique and Microscopy and he doesn't suggest any one brand of paraffin. Andi Grantham At 01:13 PM 3/27/2006 -0500, Jerry Helisek wrote: >What would the best "wax" to use for embedding Plant tissue... mainly >Corn & Soybean. > > > >Thank > > > >Jerry Helisek > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From Diane.Gladney <@t> se.amedd.army.mil Tue Mar 28 09:09:39 2006 From: Diane.Gladney <@t> se.amedd.army.mil (Gladney, Diane C Ms MACH) Date: Tue Mar 28 09:10:30 2006 Subject: [Histonet] Venting Part II Message-ID: <4D55B2E997EFAE4DA6081DDE100B8302CCFE68@amedmlsermc133.amed.ds.army.mil> You can always say what Jeff Foxworthy's cohort says: "Here's your sign!" Diane C. Gladney, HT (ASCP) Supervisor, Histology Dept. Moncrief Army Community Hospital Dept. of Pathology 4500 Stuart St. FT. Jackson, SC 29207 Phone: 803-751-3530 FAX: 803-751-7829 DSN: 734-2530 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Thursday, April 27, 2006 9:23 PM To: histonet Subject: [Histonet] Venting Part II Okay, as I said in my apology posting, I used "dumb ass" to describe the plumber who did not want to vent the flame cabinets. Please give me an alternative phrase for me to use because I don't understand the following events: 1. what do you call it when you put in a drench shower/eyewash station combo, but don't put in a drain? 2. what do you call it when you leave a 2" pipe open near the bottom of the shower/eyewash so that when you test the eyewash station, a rush of water soaks your shoes? 3. what do you call it when you put in a deionized water station, but don't know what a conductivity light is? So when you ask the plumber "how do you when to change the tank if there is no light", and the plumber just says "I don't know"?. 4. what do you call it when you blow a test ball through the drain system to test the drain system, yet forget to take the test ball out of the drain and water backs up into your new lab causing a huge flood in the lab? Then have to call in a competitor to help you fix it. 5. what do you call it when you try to light a gas water heater for 30 minutes, just to be told by some one else that there is no gas meter hooked up because you forgot to get the meter from the city? 6. what do you call it when you are soldering a pipe and activate the fire alarm system? I fell like Jeff Foxworthy "You might be a redneck if". Can some one please come up with an alternative phrase other than "dumb ass"? These events really happened this weekend in my new $1.5 million lab. I know, it sounds like fiction, but trust me, it's the real thing. So you see my dilemma. And for that one person who I offended in the past, I may be unprofessional in my language and conduct, but I do know MY job. Thanks for listening. In advance, I am sorry for the harsh language once again, but I can't come up with the words to accurately describe this "professional" plumber. Let the flaming begin (I'll just have to get another plumber to help put out the flames) Joe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pathrm35 <@t> adelphia.net Tue Mar 28 09:11:45 2006 From: pathrm35 <@t> adelphia.net (pathrm35@adelphia.net) Date: Tue Mar 28 09:11:53 2006 Subject: [Histonet] Venting Part II Message-ID: <9598424.1143558705623.JavaMail.root@web22> ---- Jackie M O'Connor wrote: > I think you call that a Union Man. > Joe, I went through this when we built our new ab last year. I dumped all these issues off on the general contractor. But then again, I was the on who had to put in the ceiling tiles among other things. Building a new lab is a very time consuming and mentally consuming event. We had excellent plumbers but we sure did pay for them. Ron Martin > > > > > > "Joe Nocito" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 04/27/2006 08:23 PM > > > To: "histonet" > cc: (bcc: Jackie M O'Connor/LAKE/GPRD/ABBOTT) > Subject: [Histonet] Venting Part II > > > Okay, as I said in my apology posting, I used "dumb ass" to describe > the plumber who did not want to vent the flame cabinets. Please give me an > alternative phrase for me to use because I don't understand the following > events: > 1. what do you call it when you put in a drench shower/eyewash station > combo, but don't put in a drain? > 2. what do you call it when you leave a 2" pipe open near the bottom > of the shower/eyewash so that when you test the eyewash station, a rush of > water soaks your shoes? > 3. what do you call it when you put in a deionized water station, but > don't know what a conductivity light is? So when you ask the plumber "how > do you when to change the tank if there is no light", and the plumber just > says "I don't know"?. > 4. what do you call it when you blow a test ball through the drain > system to test the drain system, yet forget to take the test ball out of > the drain and water backs up into your new lab causing a huge flood in the > lab? Then have to call in a competitor to help you fix it. > 5. what do you call it when you try to light a gas water heater for > 30 minutes, just to be told by some one else that there is no gas meter > hooked up because you forgot to get the meter from the city? > 6. what do you call it when you are soldering a pipe and activate the > fire alarm system? > > I fell like Jeff Foxworthy "You might be a redneck if". > > Can some one please come up with an alternative phrase other than "dumb > ass"? These events really happened this weekend in my new $1.5 million > lab. I know, it sounds like fiction, but trust me, it's the real thing. So > you see my dilemma. And for that one person who I offended in the past, I > may be unprofessional in my language and conduct, but I do know MY job. > > Thanks for listening. In advance, I am sorry for the harsh language once > again, but I can't come up with the words to accurately describe this > "professional" plumber. > > Let the flaming begin (I'll just have to get another plumber to help put > out the flames) > > Joe > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Tue Mar 28 09:55:00 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Tue Mar 28 09:55:10 2006 Subject: [Histonet] Eosin Y alcoholic with or without phloxine? Message-ID: <09C945920A6B654199F7A58A1D7D1FDE017176B1@lsexch.lsmaster.lifespan.org> If your procedure works well on most tissues, but doesn't work on one type of tissue from one source, I would look to the tissue, not the staining solution, for the explanation, particularly the fixative used on the rat tissues. The phloxine, when present, has no direct effect on the action of the eosin. Phloxine is just used to provide a greater range of pink hues than you can get with eosin alone. The description of the Sigma product in the catalog sounds pretty straightforward - 0.5% solution in acidified 90% alcohol. That's what I use, though I mix my own solution, and I do include phloxine. If you have a problem tissue that doesn't retain eosin well, there are a few things you can try. First, you can further acidify the eosin by adding 2 ml acetic acid per 100 ml of stain. Second, after your hematoxylin bluing step and subsequent washing, immerse the slides in 4% aqueous acetic acid for 30 to 60 seconds before going into the eosin. Third, don't leave the slides in the dehydration alcohols longer than necessary, particularly the alcohols less than absolute in concentration. Many labs use much longer dehydration times than are actually necessary. If you use 70% alcohol in your dehydration series, skip that step. Go directly from the eosin into 95% alcohol, and leave the slides in 95% only briefly (20 dips) before moving on to the absolute alcohols. 95% ethanol dissolves eosin out of tissue fairly quickly, much more quickly than absolute ethanol does. If the above steps don't solve the problem, you can try (1) using eosin with phloxine. While the phloxine doesn't affect the action of the eosin, the tissue's inability to bind eosin doesn't necessarily mean that it won't bind phloxine, so you may get a satisfactory stain using the combination of the two similar dyes. Or (2) try dehydrating with a 50:50 mix of ethanol and isopropanol instead of pure ethanol. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cathy > Malcontenti-Wilson > Sent: Monday, March 27, 2006 5:34 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Eosin Y alcoholic with or without phloxine? > > Dear All, > I would like to ask about a particular problem with eosin-Y staining. I am > > using Eosin Y alcoholic without phloxine (from Sigma) and for some reason, > > all of the eosin staining comes out of the tissue as it is being > dehydrated. I can actually see that it just drains out. I know that this > is > part of the differentiation process but it just all comes out. We use the > same staining set for mouse liver tumour tissues with no problem. These > are > routinely processed (by a hospital pathology lab) rat rectum slides. Is > the phloxine important for "keeping in the stain"? The sections are quite > small. Should I be using a different eosin? > Any advice on this matter would be greatly appreciated. > Cathy > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From leslie.duncan <@t> bms.com Tue Mar 28 10:14:42 2006 From: leslie.duncan <@t> bms.com (Leslie D Duncan) Date: Tue Mar 28 10:16:02 2006 Subject: [Histonet] GMA and Humidity? Message-ID: <442960F2.80202@bms.com> Hello, I am new to the GMA process - we just started method development about a month ago. Thank you for all of your replies regarding H&E staining. I haven't had the opportunity to try any of them yet, but it's on my list for this week. My new question is - I have been reading a lot regarding GMA and humidity. We are currently using the JB-4 kit, and have not had any problems yet; however, it's still chilly here and the humidity hasn't kicked in yet. I live in southern Indiana, the heart of humidity country in the summer and fall!! And our building facilitators sometime have trouble controlling the humidity inside when it's 90 degrees with 95% humidity outside. What type of problems should I expect, and is there something that gives less humidity problems than JB-4? Thank you, Leslie Duncan Bristol-Myers Squibb, PRI From settembr <@t> umdnj.edu Tue Mar 28 10:36:50 2006 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Tue Mar 28 10:38:11 2006 Subject: [Histonet] Insulin Growth Factor-1 help Message-ID: Hello, I have been trying to get IGF-1 to work on formalin fixed paraffin embedded human tissue and it's not working. I am using Santa Cruz' IGF-1 rabbit polyclonal, (cat# sc-9013) I have tried all sorts of retrievals and all sorts of dilutions with my anti mouse/anti rabbit universal detection kit. Things usually work one way or another and I am at my wits end. Can someone who has gotten this antibody to work on FFPE human tissue please let me know what Vendor you use, retrieval, dilution, detection system? Thank you so much for any help. Dana Settembre UMDNJ - University Hospital Newark, NJ USA From gcallis <@t> montana.edu Tue Mar 28 10:59:51 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Mar 28 11:00:03 2006 Subject: [Histonet] GMA and Humidity? In-Reply-To: <442960F2.80202@bms.com> References: <442960F2.80202@bms.com> Message-ID: <6.0.0.22.1.20060328095040.01b671a8@gemini.msu.montana.edu> The GMA will be affected by humidity to the point that softened plastic is sometimes a problem to section. Just place your blocks in a dessicator or some type of plastic box with a lid (rubbermaid, etc) containing a dessicant i.e. anhydrous calcium chloride inside a mesh bag (recycled knee high nylons are good!) contains this stuff. Let blocks sit overnight or several hours to keep plastic from water exposure. Drierite is a good dessicant , comes in bulk, and is anhydrous calcium chloride with a blue indicator so you know when it is exhausted - it turns pinkish. 16 mesh silica gel with indicator from Fisher work is another and also good and doesn't exfoliate fine CaCL2 dust. 09:14 AM 3/28/2006, you wrote: >Hello, > >I am new to the GMA process - we just started method development about a >month ago. Thank you for all of your replies regarding H&E staining. I >haven't had the opportunity to try any of them yet, but it's on my list >for this week. > >My new question is - I have been reading a lot regarding GMA and >humidity. We are currently using the JB-4 kit, and have not had any >problems yet; however, it's still chilly here and the humidity hasn't >kicked in yet. I live in southern Indiana, the heart of humidity country >in the summer and fall!! And our building facilitators sometime have >trouble controlling the humidity inside when it's 90 degrees with 95% >humidity outside. What type of problems should I expect, and is there >something that gives less humidity problems than JB-4? >Thank you, >Leslie Duncan >Bristol-Myers Squibb, PRI > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From rjbuesa <@t> yahoo.com Tue Mar 28 11:24:14 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 28 11:24:20 2006 Subject: [Histonet] Venting Part II In-Reply-To: <00c001c66a62$4aa4a040$e8bd0b43@yourxhtr8hvc4p> Message-ID: <20060328172414.45339.qmail@web61224.mail.yahoo.com> Joe: Why don't you just call him what he is: "incompetent" avoiding any more "colorful" but less accurate expletives? Besides there are very inteligent (real) asses out in the Zoo (or in the Afican savannas)! Ren? J. Joe Nocito wrote: Okay, as I said in my apology posting, I used "dumb ass" to describe the plumber who did not want to vent the flame cabinets. Please give me an alternative phrase for me to use because I don't understand the following events: 1. what do you call it when you put in a drench shower/eyewash station combo, but don't put in a drain? 2. what do you call it when you leave a 2" pipe open near the bottom of the shower/eyewash so that when you test the eyewash station, a rush of water soaks your shoes? 3. what do you call it when you put in a deionized water station, but don't know what a conductivity light is? So when you ask the plumber "how do you when to change the tank if there is no light", and the plumber just says "I don't know"?. 4. what do you call it when you blow a test ball through the drain system to test the drain system, yet forget to take the test ball out of the drain and water backs up into your new lab causing a huge flood in the lab? Then have to call in a competitor to help you fix it. 5. what do you call it when you try to light a gas water heater for 30 minutes, just to be told by some one else that there is no gas meter hooked up because you forgot to get the meter from the city? 6. what do you call it when you are soldering a pipe and activate the fire alarm system? I fell like Jeff Foxworthy "You might be a redneck if". Can some one please come up with an alternative phrase other than "dumb ass"? These events really happened this weekend in my new $1.5 million lab. I know, it sounds like fiction, but trust me, it's the real thing. So you see my dilemma. And for that one person who I offended in the past, I may be unprofessional in my language and conduct, but I do know MY job. Thanks for listening. In advance, I am sorry for the harsh language once again, but I can't come up with the words to accurately describe this "professional" plumber. Let the flaming begin (I'll just have to get another plumber to help put out the flames) Joe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1¢/min. From JPaulB42 <@t> aol.com Tue Mar 28 11:44:03 2006 From: JPaulB42 <@t> aol.com (JPaulB42@aol.com) Date: Tue Mar 28 11:44:13 2006 Subject: [Histonet] microwave processing??? Message-ID: <2c4.6571c8d.315acfe3@aol.com> Hello Fellow Histonetters: We are currently planning to bring microwave technology into our lab. The problem being no one has any experience with processing tissue with microwave. I was hoping someone could guide me to some information about this techniques and to answer other question as we go along. Any and all help would be greatly appreciated. Sincerely, Jim Bradford From Barry.R.Rittman <@t> uth.tmc.edu Tue Mar 28 12:00:58 2006 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Tue Mar 28 12:01:04 2006 Subject: [Histonet] Venting Part II Message-ID: Rene There is good news in that asses (mules) in the zoo are sterile and therefore do not reproduce. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, March 28, 2006 11:24 AM To: Joe Nocito; histonet Subject: Re: [Histonet] Venting Part II Joe: Why don't you just call him what he is: "incompetent" avoiding any more "colorful" but less accurate expletives? Besides there are very inteligent (real) asses out in the Zoo (or in the Afican savannas)! Ren? J. Joe Nocito wrote: Okay, as I said in my apology posting, I used "dumb ass" to describe the plumber who did not want to vent the flame cabinets. Please give me an alternative phrase for me to use because I don't understand the following events: 1. what do you call it when you put in a drench shower/eyewash station combo, but don't put in a drain? 2. what do you call it when you leave a 2" pipe open near the bottom of the shower/eyewash so that when you test the eyewash station, a rush of water soaks your shoes? 3. what do you call it when you put in a deionized water station, but don't know what a conductivity light is? So when you ask the plumber "how do you when to change the tank if there is no light", and the plumber just says "I don't know"?. 4. what do you call it when you blow a test ball through the drain system to test the drain system, yet forget to take the test ball out of the drain and water backs up into your new lab causing a huge flood in the lab? Then have to call in a competitor to help you fix it. 5. what do you call it when you try to light a gas water heater for 30 minutes, just to be told by some one else that there is no gas meter hooked up because you forgot to get the meter from the city? 6. what do you call it when you are soldering a pipe and activate the fire alarm system? I fell like Jeff Foxworthy "You might be a redneck if". Can some one please come up with an alternative phrase other than "dumb ass"? These events really happened this weekend in my new $1.5 million lab. I know, it sounds like fiction, but trust me, it's the real thing. So you see my dilemma. And for that one person who I offended in the past, I may be unprofessional in my language and conduct, but I do know MY job. Thanks for listening. In advance, I am sorry for the harsh language once again, but I can't come up with the words to accurately describe this "professional" plumber. Let the flaming begin (I'll just have to get another plumber to help put out the flames) Joe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1¢/min. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Mar 28 12:04:49 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 28 12:04:56 2006 Subject: [Histonet] microwave processing??? In-Reply-To: <2c4.6571c8d.315acfe3@aol.com> Message-ID: <20060328180450.19693.qmail@web61222.mail.yahoo.com> Jim: 1-If your intent is to buy a tissue processor that uses MW technology, the vendor for sure will provide you with the required protocols. 2-If you are just buying a laboratory designed MW oven to process tissue with personal intervention in each step, that is something else and you will need to use help from others that have done that. If you are going to follow "Rute 1" you have nothing to worry about, the technology works. If you are going to follow "Rute 2" besides the imput you may receive from colleagues, I would suggest to you to try to obtain the following 2 books: "The Microwave Tool Book" by Login and Dvorak. They tell you about generalities (that you need to kow) and about fixation (it does not go farther than that), and "Microwaves for the Art of Microscopy" by Kok and Boon. This is more specialized and can guide you through the whole process including staining. Following "Rute 2" will be venturous and difficult and you will need all the help you can get. There are also many discussions on the subject in Histonet archives and many publications. Hope this will help you! Ren? J. JPaulB42@aol.com wrote: Hello Fellow Histonetters: We are currently planning to bring microwave technology into our lab. The problem being no one has any experience with processing tissue with microwave. I was hoping someone could guide me to some information about this techniques and to answer other question as we go along. Any and all help would be greatly appreciated. Sincerely, Jim Bradford _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Blab-away for as little as 1?/min. Make PC-to-Phone Calls using Yahoo! Messenger with Voice. From ploykasek <@t> phenopath.com Tue Mar 28 12:26:38 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Tue Mar 28 12:27:03 2006 Subject: [Histonet] Venting Part II In-Reply-To: <00c001c66a62$4aa4a040$e8bd0b43@yourxhtr8hvc4p> Message-ID: Joe - I can truly sympathize with you since we moved into a new building about 3 yrs ago & certain contractors issues are still haunting us. Just one note - there might be a valid reason for not installing drains at the shower - you local regulations may not allow it. We don't have one because the city doesn't want any chemical contamination in their drainage system (save the salmon!). Good luck at your new location, enjoy it. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA Okay, as I said in my apology posting, I used "dumb ass" to describe the > plumber who did not want to vent the flame cabinets. Please give me an > alternative phrase for me to use because I don't understand the following > events: > 1. what do you call it when you put in a drench shower/eyewash station > combo, but don't put in a drain? > 2. what do you call it when you leave a 2" pipe open near the bottom of the > shower/eyewash so that when you test the eyewash station, a rush of water > soaks your shoes? > 3. what do you call it when you put in a deionized water station, but don't > know what a conductivity light is? So when you ask the plumber "how do you > when to change the tank if there is no light", and the plumber just says "I > don't know"?. > 4. what do you call it when you blow a test ball through the drain system to > test the drain system, yet forget to take the test ball out of the drain and > water backs up into your new lab causing a huge flood in the lab? Then have to > call in a competitor to help you fix it. > 5. what do you call it when you try to light a gas water heater for 30 > minutes, just to be told by some one else that there is no gas meter hooked up > because you forgot to get the meter from the city? > 6. what do you call it when you are soldering a pipe and activate the fire > alarm system? > > I fell like Jeff Foxworthy "You might be a redneck if". > > Can some one please come up with an alternative phrase other than "dumb ass"? > These events really happened this weekend in my new $1.5 million lab. I know, > it sounds like fiction, but trust me, it's the real thing. So you see my > dilemma. And for that one person who I offended in the past, I may be > unprofessional in my language and conduct, but I do know MY job. > > Thanks for listening. In advance, I am sorry for the harsh language once > again, but I can't come up with the words to accurately describe this > "professional" plumber. > > Let the flaming begin (I'll just have to get another plumber to help put out > the flames) > > Joe > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From pmcardle <@t> ebsciences.com Tue Mar 28 13:03:14 2006 From: pmcardle <@t> ebsciences.com (Phil McArdle) Date: Tue Mar 28 13:03:32 2006 Subject: [Histonet] microwave processing??? In-Reply-To: <2c4.6571c8d.315acfe3@aol.com> References: <2c4.6571c8d.315acfe3@aol.com> Message-ID: <44298872.4050201@ebsciences.com> Hello: Kok and Boon, absolutely. For more tips and protocols, check out the "library" section of www.ebsciences.com. Some are dated and we're constantly updating our site, but should be helpful. Best regards, Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. Tel: 800.992.9037 x 341 Cell: 860.597.6796 Fax: 860.653.0422 PMcardle@ebsciences.com www.ebsciences.com "ADDING BRILLIANCE TO YOUR VISION" "I hate quotations. Tell me what you know." Ralph Waldo Emerson JPaulB42@aol.com wrote: > Hello Fellow Histonetters: > > We are currently planning to bring microwave technology into our lab. The > problem being no one has any experience with processing tissue with microwave. I > was hoping someone could guide me to some information about this techniques > and to answer other question as we go along. Any and all help would be > greatly appreciated. > > Sincerely, > > Jim Bradford > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- From RSTAFFORD <@t> mhc.net Tue Mar 28 13:06:57 2006 From: RSTAFFORD <@t> mhc.net (Rosemary Stafford) Date: Tue Mar 28 13:07:28 2006 Subject: [Histonet] We are puzzled by CAP question ANP.21850. It suggests the use of internal controls for renal bxs. Message-ID: We are puzzled by CAP question ANP.21850. It suggests the use of internal controls for renal bxs. We do Direct Immunofluorescence on skin biopsies only. What internal controls are other people using for their skin bxs?. Also, has anyone found a source for a positive sausage control for DIF? What are other people using for their positive controls? Thanks for the help. Rosemary From vazquezr <@t> ohsu.edu Tue Mar 28 13:21:37 2006 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Tue Mar 28 13:22:02 2006 Subject: [Histonet] We are puzzled by CAP question ANP.21850. It suggests the use of internal controls for re Message-ID: rosemary, We are using known positive patients that has been read out as our internal positive controls. Robyn OHSU >>> "Rosemary Stafford" 3/28/2006 11:06 AM >>> We are puzzled by CAP question ANP.21850. It suggests the use of internal controls for renal bxs. We do Direct Immunofluorescence on skin biopsies only. What internal controls are other people using for their skin bxs?. Also, has anyone found a source for a positive sausage control for DIF? What are other people using for their positive controls? Thanks for the help. Rosemary _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From srodriguez <@t> phenopath.com Tue Mar 28 13:28:16 2006 From: srodriguez <@t> phenopath.com (Stephanie Rodriguez) Date: Tue Mar 28 13:28:42 2006 Subject: [Histonet] Re: Pax-2 (Greg Luck) In-Reply-To: Message-ID: Greg, We use a polyclonal (rabbit) anti-PAX-2 from Zymed Labs. (800-874-4494; www.zymed.com) We use it at 1:100 with a citrate (pH 6.0) pretreatment and Dako Rabbit Envision/DAB detection. Hope this helps! Stephanie Rodriguez, BS, HTL(ASCP), QIHC Phenopath Laboratories Seattle, WA (206) 374-9000 > Message: 4 > Date: Mon, 27 Mar 2006 10:34:01 -0800 > From: "Luck, Greg D." > Subject: [Histonet] PAX-2 Ab. > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Hello, > > > > Could someone supply me with the/a vendor for an antibody called "PAX-2" > for FFPE. Thanks, Greg > > > > Greg Luck > > Anatomic Pathology Supervisor > > Deaconess Med Center / EHS > > 800 W. 5th Ave > > Spokane, WA 99204 > > Phone 509.473.7394 > > Fax 509.473.7133 > > luckg@empirehealth.org ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From rjbuesa <@t> yahoo.com Tue Mar 28 13:56:52 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 28 13:56:55 2006 Subject: [Histonet] We are puzzled by CAP question ANP.21850. It suggests the use of internal controls for renal bxs. In-Reply-To: Message-ID: <20060328195652.75356.qmail@web61220.mail.yahoo.com> Rosemary: I used FS sections of positive skin cases kept at -80?C until needed. I kept renewing the controls with new cases when the controls were all used. Ren? J. Rosemary Stafford wrote: We are puzzled by CAP question ANP.21850. It suggests the use of internal controls for renal bxs. We do Direct Immunofluorescence on skin biopsies only. What internal controls are other people using for their skin bxs?. Also, has anyone found a source for a positive sausage control for DIF? What are other people using for their positive controls? Thanks for the help. Rosemary _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- New Yahoo! Messenger with Voice. Call regular phones from your PC and save big. From kbroomal <@t> NEMOURS.ORG Tue Mar 28 14:15:58 2006 From: kbroomal <@t> NEMOURS.ORG (Kristen Broomall) Date: Tue Mar 28 14:16:22 2006 Subject: [Histonet] Venting Part II Message-ID: <6E41111281623B4B8A9AB8F9A7EA3437824505@wlmmsx02.nemours.org> I'll forward that to my very hardworking, intelligent, and diligent boyfriend who is a "Union Man". Perhaps dumbass is a better description for a single person who is not a good worker than to generalize that an entire group of people are incompetent. Kristen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jackie M O'Connor Sent: Tuesday, March 28, 2006 8:05 AM To: Joe Nocito Cc: histonet-bounces@lists.utsouthwestern.edu; histonet Subject: Re: [Histonet] Venting Part II I think you call that a Union Man. "Joe Nocito" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/27/2006 08:23 PM To: "histonet" cc: (bcc: Jackie M O'Connor/LAKE/GPRD/ABBOTT) Subject: [Histonet] Venting Part II Okay, as I said in my apology posting, I used "dumb ass" to describe the plumber who did not want to vent the flame cabinets. Please give me an alternative phrase for me to use because I don't understand the following events: 1. what do you call it when you put in a drench shower/eyewash station combo, but don't put in a drain? 2. what do you call it when you leave a 2" pipe open near the bottom of the shower/eyewash so that when you test the eyewash station, a rush of water soaks your shoes? 3. what do you call it when you put in a deionized water station, but don't know what a conductivity light is? So when you ask the plumber "how do you when to change the tank if there is no light", and the plumber just says "I don't know"?. 4. what do you call it when you blow a test ball through the drain system to test the drain system, yet forget to take the test ball out of the drain and water backs up into your new lab causing a huge flood in the lab? Then have to call in a competitor to help you fix it. 5. what do you call it when you try to light a gas water heater for 30 minutes, just to be told by some one else that there is no gas meter hooked up because you forgot to get the meter from the city? 6. what do you call it when you are soldering a pipe and activate the fire alarm system? I fell like Jeff Foxworthy "You might be a redneck if". Can some one please come up with an alternative phrase other than "dumb ass"? These events really happened this weekend in my new $1.5 million lab. I know, it sounds like fiction, but trust me, it's the real thing. So you see my dilemma. And for that one person who I offended in the past, I may be unprofessional in my language and conduct, but I do know MY job. Thanks for listening. In advance, I am sorry for the harsh language once again, but I can't come up with the words to accurately describe this "professional" plumber. Let the flaming begin (I'll just have to get another plumber to help put out the flames) Joe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hej01 <@t> health.state.ny.us Tue Mar 28 14:31:11 2006 From: hej01 <@t> health.state.ny.us (Helen E Johnson) Date: Tue Mar 28 14:31:30 2006 Subject: [Histonet] Martius Scarlet Blue Method Message-ID: Hi Histonetters, Does anyone know if there is a vendor that sells a kit for the Martius Scarlet Blue method. Thanks. Helen (hej01@health.state.ny.us) From LGaliotto <@t> nch.org Tue Mar 28 15:04:37 2006 From: LGaliotto <@t> nch.org (Galiotto, Laura) Date: Tue Mar 28 15:04:41 2006 Subject: [Histonet] Cryojane Message-ID: <270614B321ACB44D8C1D91F4F921FDC3036CBC6B@NCH01EX02.nch.org> Can anyone give pros and cons with CryoJane (frozensections) especially concerns with Liquid Nitrogen. Laura Galiotto, HT (ASCP) Histology Facilitator Northwest Community Hospital 800 West Central Road Arlington Heights, Il 60005 847-618-6190 From hts <@t> gator.net Tue Mar 28 15:12:41 2006 From: hts <@t> gator.net (hts@gator.net) Date: Tue Mar 28 15:12:44 2006 Subject: [Histonet] Looking for jobs in Colorado Message-ID: <1581.65.81.70.231.1143580361.squirrel@webmail.gru.net> My wife and I are looking for histotech positions in the Boulder/Fort Collins area. Melissa has 2 1/2 years experience and Paul has 1 3/4 years experience. We should both be licensed HT(ASCP) by mid-May. We have both passed the written and our practical has been sent off and is due to be graded in April. We currently work in a reference lab in Gainesville, FL. We have a wide range of experience in research, vet-path and special projects. Paul has experience in grossing and Melissa is very proficient in microtomy. Thank you, Paul Cremers From asmith <@t> mail.barry.edu Tue Mar 28 15:32:47 2006 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Tue Mar 28 15:32:53 2006 Subject: [Histonet] Venting Part II Message-ID: <5D2189E74151CC42BEC02906BA8996322B91B0@exchsrv01.barrynet.barry.edu> The city isn't thinking very hard. 1/2 inch of water over the floor is going to be mopped up into a bucket. Guess where the bucket will be emptied? Emergency procedures are not the same as routine procedures. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Tuesday, March 28, 2006 1:27 PM To: Joe Nocito; histonet Subject: Re: [Histonet] Venting Part II Joe - I can truly sympathize with you since we moved into a new building about 3 yrs ago & certain contractors issues are still haunting us. Just one note - there might be a valid reason for not installing drains at the shower - you local regulations may not allow it. We don't have one because the city doesn't want any chemical contamination in their drainage system (save the salmon!). Good luck at your new location, enjoy it. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA Okay, as I said in my apology posting, I used "dumb ass" to describe the > plumber who did not want to vent the flame cabinets. Please give me an > alternative phrase for me to use because I don't understand the following > events: > 1. what do you call it when you put in a drench shower/eyewash station > combo, but don't put in a drain? > 2. what do you call it when you leave a 2" pipe open near the bottom of the > shower/eyewash so that when you test the eyewash station, a rush of water > soaks your shoes? > 3. what do you call it when you put in a deionized water station, but don't > know what a conductivity light is? So when you ask the plumber "how do you > when to change the tank if there is no light", and the plumber just says "I > don't know"?. > 4. what do you call it when you blow a test ball through the drain system to > test the drain system, yet forget to take the test ball out of the drain and > water backs up into your new lab causing a huge flood in the lab? Then have to > call in a competitor to help you fix it. > 5. what do you call it when you try to light a gas water heater for 30 > minutes, just to be told by some one else that there is no gas meter hooked up > because you forgot to get the meter from the city? > 6. what do you call it when you are soldering a pipe and activate the fire > alarm system? > > I fell like Jeff Foxworthy "You might be a redneck if". > > Can some one please come up with an alternative phrase other than "dumb ass"? > These events really happened this weekend in my new $1.5 million lab. I know, > it sounds like fiction, but trust me, it's the real thing. So you see my > dilemma. And for that one person who I offended in the past, I may be > unprofessional in my language and conduct, but I do know MY job. > > Thanks for listening. In advance, I am sorry for the harsh language once > again, but I can't come up with the words to accurately describe this > "professional" plumber. > > Let the flaming begin (I'll just have to get another plumber to help put out > the flames) > > Joe > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From Claudia.Freyer <@t> princehenrys.org Tue Mar 28 15:42:46 2006 From: Claudia.Freyer <@t> princehenrys.org (Claudia Freyer) Date: Tue Mar 28 15:43:14 2006 Subject: [Histonet] mouse antibodies on mouse tissue Message-ID: Dear Histonetter, I am confused about the use of monoclonal antibodies produced in mice against endogenous proteins in mouse tissue. I thought an animal would not produce antibodies against its own proteins, since this would cause an autoimmune response. Would anybody know why mouse monoclonals detect epitopes on mouse proteins? Many thanks. Claudia Dr Claudia Freyer Prince Henry's Institute of Medical Research Victoria Australia From Amanda.Garcia <@t> TriadHospitals.com Tue Mar 28 15:48:43 2006 From: Amanda.Garcia <@t> TriadHospitals.com (Garcia, Amanda) Date: Tue Mar 28 15:48:48 2006 Subject: [Histonet] Histology lab Message-ID: <8B63039C9DF4554C8FDBF31235F0E148012069A0@CPRTEVS02.triadhospitals.net> We are going to be moving our histology lab to an area that is 9 x 11 (yes, that's right). The equipment that we have is one microtome ( and all the accessories that go along with it), one embedding unit, one processor (vip bench model), a grossing station, computer, printer, specimen storage, manual coverslipping station & manual staining station. I am pretty sure I didn't leave anything out but if I did, hopefully it's not anything too big. Any input on space saving design would be appreciated. Thanks, Amy > Amanda (Amy) Garcia > Histology/Pathology > College Station Medical Center > (979) 680-5372 office > (979) 696-5446 fax > *Mailto:amanda.garcia@triadhospitals.com > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the e-mail or any > of its attachments, please be advised that you have received this > e-mail in error and that any use, dissemination, distribution, > forwarding, printing, or copying of this e-mail or any attached files > is strictly prohibited. If you have received this e-mail in error, > please immediately purge it and all attachments and notify the sender > by reply e-mail or contact the sender at the number listed. > > > From rjbuesa <@t> yahoo.com Tue Mar 28 16:13:14 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Mar 28 16:13:18 2006 Subject: [Histonet] Histology lab In-Reply-To: <8B63039C9DF4554C8FDBF31235F0E148012069A0@CPRTEVS02.triadhospitals.net> Message-ID: <20060328221314.29666.qmail@web61220.mail.yahoo.com> Amanda: Try to have a long working table all along the walls from and to the door opening. You could add a small "protruding" table just in front of the door to half the width of the room to increase the bench area and that will also serve as a "division" between grossing/processing/embedding activities from sectioning/staining/coverslipping in the other. If you want I could draw a small scheme for you. Many, I really mean many years ago, I used to work in a smaller space and I had an annual workload of about 8000 blocks. Hope this will help! Ren? J. "Garcia, Amanda" wrote: We are going to be moving our histology lab to an area that is 9 x 11 (yes, that's right). The equipment that we have is one microtome ( and all the accessories that go along with it), one embedding unit, one processor (vip bench model), a grossing station, computer, printer, specimen storage, manual coverslipping station & manual staining station. I am pretty sure I didn't leave anything out but if I did, hopefully it's not anything too big. Any input on space saving design would be appreciated. Thanks, Amy > Amanda (Amy) Garcia > Histology/Pathology > College Station Medical Center > (979) 680-5372 office > (979) 696-5446 fax > *Mailto:amanda.garcia@triadhospitals.com > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the e-mail or any > of its attachments, please be advised that you have received this > e-mail in error and that any use, dissemination, distribution, > forwarding, printing, or copying of this e-mail or any attached files > is strictly prohibited. If you have received this e-mail in error, > please immediately purge it and all attachments and notify the sender > by reply e-mail or contact the sender at the number listed. > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Blab-away for as little as 1?/min. Make PC-to-Phone Calls using Yahoo! Messenger with Voice. From davmpec <@t> hotmail.com Tue Mar 28 17:58:26 2006 From: davmpec <@t> hotmail.com (David M. Peck) Date: Tue Mar 28 17:58:31 2006 Subject: [Histonet] MAK 6 HELP PLEASE!!!!!!!!!!! In-Reply-To: Message-ID: Jesus I have usedMAK 6 in the past and I simply used Ventana Protease 1 for 6 minutes and then used an apprioate bilution/incubation time for the primary and it was no problem. David Peck HT,HTL(ASCP) >From: "Jesus Ellin" >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] MAK 6 HELP PLEASE!!!!!!!!!!! >Date: Thu, 23 Mar 2006 05:34:29 -0700 > >Hello everyone I need some really big help we are trying to add an >additional antibody to our system MAK6 from Zymed. We are using Ventana >stainers, one is a Benchmark the other is LT. If anyone out there is using >this antibody on these stainers and having success, PLEASE!!!!!!!!!!!! let >me have your protocol for pariffin embeded and formalin fixed tissue. > > >Jesus Ellin >Yuma Regional Medical Center > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Malcolm.McCallum <@t> tamut.edu Tue Mar 28 18:13:11 2006 From: Malcolm.McCallum <@t> tamut.edu (Malcolm McCallum) Date: Tue Mar 28 18:13:42 2006 Subject: [Histonet] Faculty Vacancy Announcement Message-ID: If you are looking for a faculty position. The below vacancy is currently available at my institution. People are nice, we are building a new science building, and have major expansion planned due (in part) to major investments by a local Lawyer and Ross Perot. Check out the link: http://www.tamut.edu/endow/index.html Hope this helps one of you folks out! Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-233-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html Texas A&M University-Texarkana Position Announcement Assistant/Associate Professor of Biology Required qualifications: * An earned doctorate in Biology or a closely related field. Candidates with evidence of completion of all degree requirements prior to employment will be evaluated, but, employment is contingent upon award of the doctorate. * A minimum of one year teaching experience at the community or senior college level. Preferred qualifications: * Evidence of significant teaching experience at the university level. * Record of research & scholarly activity. * Involvement in professional organizations. * Experience in acquiring external funding and/or research grants appropriate to the rank of an assistant professor. * Record of advising students on research projects leading to publications or presentations at scientific meetings. * Record of teaching and researching in one or more areas of Organismal Biology (Herpetology, Mammalogy, Ichthyology, Field Biology). * Ability to teach a broad range of courses, including but not limited to Genetics, Cell Biology, and General Ecology. Responsibilities: * Teach undergraduate biology courses including Herpetology, Mammalogy, Ichthyology, Field Biology, Genetics, Cell Biology, General Ecology, Vertebrate Histology, Parasitology, and Forensic Science. * Maintain an active research program leading to publication and acquiring research funding. * Advise and supervise undergraduate students in projects and research activities. * Perform appropriate levels of service within the university, regionally, and/or nationally. Salary: Competitive and commensurate with experience and rank. Projected Starting Date Date: Fall 2006 Semester. Position will remain open until filled. However, the screening of completed applications will begin on November 1, 2005. Application Requirements: Letter of interest, current curriculum vitae, copies of university transcripts (official transcripts required prior to employment), and three letters of current professional reference. Material should be submitted to: Dr. David W. Allard Texas A&M University-Texarkana P.O. Box 5518, 2600 North Robison Road Texarkana, TX 75505-5518 david.allard@tamut.edu 903-223-3131 Location and Environment: A&M-Texarkana is currently an upper-division and graduate public institution with an academic enrollment of approximately 1,650 students. Texas House Bill 1566 authorizes the University to admit freshmen and sophomores as soon as we move to our new campus. The University is located in Texarkana, Texas, approximately half way between Dallas, Texas, and Little Rock, Arkansas. The State of Texas requires Selective Service registration or exemption for all newly hired employees. Texas A&M University-Texarkana is an Equal Opportunity Employer. This is a security sensitive position. Criminal background check will be conducted on finalists. Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-233-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html From jnocito <@t> satx.rr.com Tue Mar 28 19:34:49 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Mar 28 19:34:50 2006 Subject: [Histonet] Venting Part II References: Message-ID: <009b01c66b24$b47be150$e8bd0b43@yourxhtr8hvc4p> All the lab water waste is sent to a dilution tank so as not to contaminate anything in case of a water pipe breakage, or here in San Antonio when we have flash floods, everything mixes with everything.. Joe ----- Original Message ----- From: "Patti Loykasek" To: "Joe Nocito" ; "histonet" Sent: Tuesday, March 28, 2006 1:26 PM Subject: Re: [Histonet] Venting Part II > Joe - I can truly sympathize with you since we moved into a new building > about 3 yrs ago & certain contractors issues are still haunting us. Just > one > note - there might be a valid reason for not installing drains at the > shower > - you local regulations may not allow it. We don't have one because the > city > doesn't want any chemical contamination in their drainage system (save the > salmon!). > Good luck at your new location, enjoy it. > > > Patti Loykasek BS, HTL, QIHC > PhenoPath Laboratories > Seattle, WA > > > > > Okay, as I said in my apology posting, I used "dumb ass" to describe the >> plumber who did not want to vent the flame cabinets. Please give me an >> alternative phrase for me to use because I don't understand the following >> events: >> 1. what do you call it when you put in a drench shower/eyewash station >> combo, but don't put in a drain? >> 2. what do you call it when you leave a 2" pipe open near the bottom >> of the >> shower/eyewash so that when you test the eyewash station, a rush of water >> soaks your shoes? >> 3. what do you call it when you put in a deionized water station, but >> don't >> know what a conductivity light is? So when you ask the plumber "how do >> you >> when to change the tank if there is no light", and the plumber just says >> "I >> don't know"?. >> 4. what do you call it when you blow a test ball through the drain >> system to >> test the drain system, yet forget to take the test ball out of the drain >> and >> water backs up into your new lab causing a huge flood in the lab? Then >> have to >> call in a competitor to help you fix it. >> 5. what do you call it when you try to light a gas water heater for 30 >> minutes, just to be told by some one else that there is no gas meter >> hooked up >> because you forgot to get the meter from the city? >> 6. what do you call it when you are soldering a pipe and activate the >> fire >> alarm system? >> >> I fell like Jeff Foxworthy "You might be a redneck if". >> >> Can some one please come up with an alternative phrase other than "dumb >> ass"? >> These events really happened this weekend in my new $1.5 million lab. I >> know, >> it sounds like fiction, but trust me, it's the real thing. So you see my >> dilemma. And for that one person who I offended in the past, I may be >> unprofessional in my language and conduct, but I do know MY job. >> >> Thanks for listening. In advance, I am sorry for the harsh language once >> again, but I can't come up with the words to accurately describe this >> "professional" plumber. >> >> Let the flaming begin (I'll just have to get another plumber to help put >> out >> the flames) >> >> Joe >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------------------------------------------------- > This e-mail message, including any attachments, is for the sole use of > the intended recipients and may contain privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If > you are not the intended recipient, please contact the sender by e-mail > and destroy all copies of the original message, or you may call PhenoPath > Laboratories, Seattle, WA U.S.A. at (206) 374-9000. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > Internal Virus Database is out-of-date. > Checked by AVG Free Edition. > Version: 7.1.385 / Virus Database: 268.3.2/294 - Release Date: 3/27/2006 > > From jnocito <@t> satx.rr.com Tue Mar 28 19:37:02 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Mar 28 19:37:04 2006 Subject: [Histonet] Venting Part II References: <20060328172414.45339.qmail@web61224.mail.yahoo.com> Message-ID: <00a701c66b25$068af5d0$e8bd0b43@yourxhtr8hvc4p> but incompetent just doesn't give the same vivid picture ----- Original Message ----- From: "Rene J Buesa" To: "Joe Nocito" ; "histonet" Sent: Tuesday, March 28, 2006 12:24 PM Subject: Re: [Histonet] Venting Part II > Joe: > Why don't you just call him what he is: "incompetent" avoiding any more > "colorful" but less accurate expletives? > Besides there are very inteligent (real) asses out in the Zoo (or in the > Afican savannas)! > Ren? J. > > Joe Nocito wrote: > Okay, as I said in my apology posting, I used "dumb ass" to describe the > plumber who did not want to vent the flame cabinets. Please give me an > alternative phrase for me to use because I don't understand the following > events: > 1. what do you call it when you put in a drench shower/eyewash station > combo, but don't put in a drain? > 2. what do you call it when you leave a 2" pipe open near the bottom of > the shower/eyewash so that when you test the eyewash station, a rush of > water soaks your shoes? > 3. what do you call it when you put in a deionized water station, but > don't know what a conductivity light is? So when you ask the plumber "how > do you when to change the tank if there is no light", and the plumber just > says "I don't know"?. > 4. what do you call it when you blow a test ball through the drain system > to test the drain system, yet forget to take the test ball out of the > drain and water backs up into your new lab causing a huge flood in the > lab? Then have to call in a competitor to help you fix it. > 5. what do you call it when you try to light a gas water heater for 30 > minutes, just to be told by some one else that there is no gas meter > hooked up because you forgot to get the meter from the city? > 6. what do you call it when you are soldering a pipe and activate the fire > alarm system? > > I fell like Jeff Foxworthy "You might be a redneck if". > > Can some one please come up with an alternative phrase other than "dumb > ass"? These events really happened this weekend in my new $1.5 million > lab. I know, it sounds like fiction, but trust me, it's the real thing. So > you see my dilemma. And for that one person who I offended in the past, I > may be unprofessional in my language and conduct, but I do know MY job. > > Thanks for listening. In advance, I am sorry for the harsh language once > again, but I can't come up with the words to accurately describe this > "professional" plumber. > > Let the flaming begin (I'll just have to get another plumber to help put > out the flames) > > Joe > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great > rates starting at 1¢/min. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > Internal Virus Database is out-of-date. > Checked by AVG Free Edition. > Version: 7.1.385 / Virus Database: 268.3.2/294 - Release Date: 3/27/2006 > > From louise.renton <@t> gmail.com Wed Mar 29 04:10:18 2006 From: louise.renton <@t> gmail.com (louise renton) Date: Wed Mar 29 05:10:36 2006 Subject: [Histonet] BSA in PBS Message-ID: Hello all, would someone please remind me of the concentration of Bovine serum albumin to put in PBS buffer to reduce background staining (IHC)? Also, if I don't have a microwave, pressure cooker, rice/vegetable steamer, or autoclave - whats another way to do heat mediated antigen retrieval? Best regards and thanks in advance -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "Does a conceited cowboy, only ride on pompous grass?. From rjbuesa <@t> yahoo.com Wed Mar 29 07:30:27 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 29 07:30:32 2006 Subject: [Histonet] BSA in PBS In-Reply-To: Message-ID: <20060329133027.84261.qmail@web61225.mail.yahoo.com> Hello Louise: Bovine albumen at 1% but if you are going to prepare a solution that will last you for more than 1 week it would be good to add 100 mg of sodium azide. Also the PBS should have 0.05% Tween 20 The only thing that I could recommend is lieu of all the tings you mention is to have a large metal pot filled with water and a strong heat source (like a Bunsen burner). Place the container with the buffer (without the slides) and heat the water to boiling point. When both the water in the pot and the buffer in the container reach isothermal conditions (100?C depending on atmospheric pressure) add the slides. Make sure that there is enough water in the pot to keep the water boiling through the duration of HIER. This (very old) arrangement for a water bath is known in some countries as "Mary (the alchemist) bath" and for sure will allow a good and isothermic HIER. It will require more time, though. Hope this will help you! Ren? J. louise renton wrote: Hello all, would someone please remind me of the concentration of Bovine serum albumin to put in PBS buffer to reduce background staining (IHC)? Also, if I don't have a microwave, pressure cooker, rice/vegetable steamer, or autoclave - whats another way to do heat mediated antigen retrieval? Best regards and thanks in advance -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "Does a conceited cowboy, only ride on pompous grass?. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. PC-to-Phone calls for ridiculously low rates. From GDawson <@t> dynacaremilwaukee.com Wed Mar 29 07:52:52 2006 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Wed Mar 29 07:52:48 2006 Subject: [Histonet] Venting Part II Message-ID: UHHHGG!!!! Kristen, Joe is making a general popular culture statement that is mixed with a bit of humor. Believe me, people are so easily offended on this list that the last thing we need is people to be getting offended on behalf of others as well. Political Correctness is the enemy of us all. When I see a word like dumbass on a post, quite honestly, it is a breath of fresh air. Everyone needs to thicken up their skins a bit before the rest of the world finds out just how WEAK we have all become. Glen Dawson IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Kristen Broomall Sent: Tuesday, March 28, 2006 2:16 PM To: 'Jackie M O'Connor'; Joe Nocito Cc: histonet-bounces@lists.utsouthwestern.edu; histonet Subject: RE: [Histonet] Venting Part II I'll forward that to my very hardworking, intelligent, and diligent boyfriend who is a "Union Man". Perhaps dumbass is a better description for a single person who is not a good worker than to generalize that an entire group of people are incompetent. Kristen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jackie M O'Connor Sent: Tuesday, March 28, 2006 8:05 AM To: Joe Nocito Cc: histonet-bounces@lists.utsouthwestern.edu; histonet Subject: Re: [Histonet] Venting Part II I think you call that a Union Man. "Joe Nocito" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/27/2006 08:23 PM To: "histonet" cc: (bcc: Jackie M O'Connor/LAKE/GPRD/ABBOTT) Subject: [Histonet] Venting Part II Okay, as I said in my apology posting, I used "dumb ass" to describe the plumber who did not want to vent the flame cabinets. Please give me an alternative phrase for me to use because I don't understand the following events: 1. what do you call it when you put in a drench shower/eyewash station combo, but don't put in a drain? 2. what do you call it when you leave a 2" pipe open near the bottom of the shower/eyewash so that when you test the eyewash station, a rush of water soaks your shoes? 3. what do you call it when you put in a deionized water station, but don't know what a conductivity light is? So when you ask the plumber "how do you when to change the tank if there is no light", and the plumber just says "I don't know"?. 4. what do you call it when you blow a test ball through the drain system to test the drain system, yet forget to take the test ball out of the drain and water backs up into your new lab causing a huge flood in the lab? Then have to call in a competitor to help you fix it. 5. what do you call it when you try to light a gas water heater for 30 minutes, just to be told by some one else that there is no gas meter hooked up because you forgot to get the meter from the city? 6. what do you call it when you are soldering a pipe and activate the fire alarm system? I fell like Jeff Foxworthy "You might be a redneck if". Can some one please come up with an alternative phrase other than "dumb ass"? These events really happened this weekend in my new $1.5 million lab. I know, it sounds like fiction, but trust me, it's the real thing. So you see my dilemma. And for that one person who I offended in the past, I may be unprofessional in my language and conduct, but I do know MY job. Thanks for listening. In advance, I am sorry for the harsh language once again, but I can't come up with the words to accurately describe this "professional" plumber. Let the flaming begin (I'll just have to get another plumber to help put out the flames) Joe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Malcolm.McCallum <@t> tamut.edu Wed Mar 29 07:58:19 2006 From: Malcolm.McCallum <@t> tamut.edu (Malcolm McCallum) Date: Wed Mar 29 07:59:19 2006 Subject: [Histonet] RE: Faculty Vacancy Announcement (revised) Message-ID: I accidentally posted a previous advertisement instead of the current vacancy. The correct post is below! Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-233-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html Position Announcement Assistant Professor of Biology Texas A&M University-Texarkana (A&M-Texarkana), a member of The Texas A&M University System, invites applications for an Assistant Professor of Biology Position with an effective target date of Fall 2006 Semester. This is a full-time, 9-month, tenure-track position which will be located on the A&M-Texarkana branch campus at Northeast Texas Community College near Mount Pleasant, Texas. Qualifications: Required * An earned doctorate in Biology or a closely related field. Candidates with evidence of completion of all degree requirements prior to employment will be evaluated, but, employment is contingent upon award of the doctorate. Preferred * Equivalent of one year teaching experience at the community or senior college level * Record of research & scholarly activity * Involvement in professional organizations * Experience in acquiring external funding and/or research grants appropriate to the rank of an assistant professor * Record of advising students on research projects leading to publications or presentations at scientific meetings * Ability to teach a broad range of courses, including but not limited to Genetics, Cell Biology, Invertebrate Zoology General Ecology, General Biology, Microbiology and Human Anatomy and Physiology Responsibilities * This position will involve teaching lower-level classes for Northeast Texas Community College (such as General Biology, Microbiology and Human Anatomy and Physiology) and upper-level classes for A&M-Texarkana (such as Genetics, Cell Biology, Invertebrate Zoology, and General Ecology). * Maintain an active research program leading to publication and acquiring research funding * Advise and supervise undergraduate students in projects and research activities * Perform appropriate levels of service within the university, regionally, and/or nationally Nine-Month Salary: Competitive and based on experience and rank. Projected Starting Date: Fall 2006 Semester. Position will remain open until filled. However, the screening of completed applications will begin on May 1, 2006. Application Requirements: Letter of interest, current curriculum vitae, copies of university transcripts (official transcripts required prior to employment), and three letters of current professional reference.. Contact Person: Dr. David Allard Texas A&M University-Texarkana P.O. Box 5518 Texarkana, Texas 75505-5518 903-223-3131. E-Mail: david.allard@tamut.edu Location and Environment: A&M-Texarkana is currently an upper-division and graduate public institution with an academic enrollment of approximately 1,600 students. Texas House Bill 1566 authorizes the University to admit freshmen and sophomores as soon as we move to our new campus. The University is located in Texarkana, Texas, approximately half way between Dallas, Texas, and Little Rock, Arkansas. Texas A&M University-Texarkana is an Equal Opportunity Employer. Security Sensitive: The State of Texas requires Selective Service registration or exemption for all newly hired employees. This is a security sensitive position. Criminal background check will be conducted on finalists. From limla <@t> mail.nih.gov Wed Mar 29 08:13:33 2006 From: limla <@t> mail.nih.gov (Lim, Langston (NIH/NCI) [E]) Date: Wed Mar 29 08:13:41 2006 Subject: [Histonet] Venting Part II Message-ID: Let's put this to rest already! Joe made a poor choice of words. He apologized, let's all be the professionals that we are and move on. Langston Lim, HT (ASCP) DHHS/NIH/NCI/Laboratory of Pathology Tissue Array Research Program -----Original Message----- From: Dawson, Glen [mailto:GDawson@dynacaremilwaukee.com] Sent: Wednesday, March 29, 2006 8:53 AM Cc: histonet-bounces@lists.utsouthwestern.edu; histonet Subject: RE: [Histonet] Venting Part II UHHHGG!!!! Kristen, Joe is making a general popular culture statement that is mixed with a bit of humor. Believe me, people are so easily offended on this list that the last thing we need is people to be getting offended on behalf of others as well. Political Correctness is the enemy of us all. When I see a word like dumbass on a post, quite honestly, it is a breath of fresh air. Everyone needs to thicken up their skins a bit before the rest of the world finds out just how WEAK we have all become. Glen Dawson IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Kristen Broomall Sent: Tuesday, March 28, 2006 2:16 PM To: 'Jackie M O'Connor'; Joe Nocito Cc: histonet-bounces@lists.utsouthwestern.edu; histonet Subject: RE: [Histonet] Venting Part II I'll forward that to my very hardworking, intelligent, and diligent boyfriend who is a "Union Man". Perhaps dumbass is a better description for a single person who is not a good worker than to generalize that an entire group of people are incompetent. Kristen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jackie M O'Connor Sent: Tuesday, March 28, 2006 8:05 AM To: Joe Nocito Cc: histonet-bounces@lists.utsouthwestern.edu; histonet Subject: Re: [Histonet] Venting Part II I think you call that a Union Man. "Joe Nocito" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/27/2006 08:23 PM To: "histonet" cc: (bcc: Jackie M O'Connor/LAKE/GPRD/ABBOTT) Subject: [Histonet] Venting Part II Okay, as I said in my apology posting, I used "dumb ass" to describe the plumber who did not want to vent the flame cabinets. Please give me an alternative phrase for me to use because I don't understand the following events: 1. what do you call it when you put in a drench shower/eyewash station combo, but don't put in a drain? 2. what do you call it when you leave a 2" pipe open near the bottom of the shower/eyewash so that when you test the eyewash station, a rush of water soaks your shoes? 3. what do you call it when you put in a deionized water station, but don't know what a conductivity light is? So when you ask the plumber "how do you when to change the tank if there is no light", and the plumber just says "I don't know"?. 4. what do you call it when you blow a test ball through the drain system to test the drain system, yet forget to take the test ball out of the drain and water backs up into your new lab causing a huge flood in the lab? Then have to call in a competitor to help you fix it. 5. what do you call it when you try to light a gas water heater for 30 minutes, just to be told by some one else that there is no gas meter hooked up because you forgot to get the meter from the city? 6. what do you call it when you are soldering a pipe and activate the fire alarm system? I fell like Jeff Foxworthy "You might be a redneck if". Can some one please come up with an alternative phrase other than "dumb ass"? These events really happened this weekend in my new $1.5 million lab. I know, it sounds like fiction, but trust me, it's the real thing. So you see my dilemma. And for that one person who I offended in the past, I may be unprofessional in my language and conduct, but I do know MY job. Thanks for listening. In advance, I am sorry for the harsh language once again, but I can't come up with the words to accurately describe this "professional" plumber. Let the flaming begin (I'll just have to get another plumber to help put out the flames) Joe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> Stowers-Institute.org Wed Mar 29 08:32:59 2006 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Wed Mar 29 08:33:24 2006 Subject: [Histonet] RE: Mouse antibodies on mouse tissue References: Message-ID: Dear Claudia, I think your confusion might be due to the fact the monoclonal antibodies produced in mice are generally against HUMAN proteins. Using them on mouse tissue relies on interspecies protein homology, i.e. the amino acid structure is similiar enough to allow binding. Most anti-mouse antibodies (created by injecting mouse proteins) are made in rat. Does this help? Teri Johnson Stowers Institute for Medical Research Kansas City, MO 64110 From kbroomal <@t> NEMOURS.ORG Wed Mar 29 08:37:59 2006 From: kbroomal <@t> NEMOURS.ORG (Kristen Broomall) Date: Wed Mar 29 08:38:20 2006 Subject: [Histonet] Venting Part II Message-ID: <6E41111281623B4B8A9AB8F9A7EA3437824509@wlmmsx02.nemours.org> Don't flame me for defending union workers. I have no problem with Joe or anyone else who needs to accurately describe poor workers with colorful language (and I don't think dumb ass is all that bad!). Where did you get that from? Did I write invisible words against Joe that only you can read? Why don't you say something to Jackie who just offended everyone involved with unions? From the messages sent to me thanking me for speaking up, I can tell you that her comment annoyed more people than just me. Kristen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Dawson, Glen Sent: Wednesday, March 29, 2006 8:53 AM Cc: histonet-bounces@lists.utsouthwestern.edu; histonet Subject: RE: [Histonet] Venting Part II UHHHGG!!!! Kristen, Joe is making a general popular culture statement that is mixed with a bit of humor. Believe me, people are so easily offended on this list that the last thing we need is people to be getting offended on behalf of others as well. Political Correctness is the enemy of us all. When I see a word like dumbass on a post, quite honestly, it is a breath of fresh air. Everyone needs to thicken up their skins a bit before the rest of the world finds out just how WEAK we have all become. Glen Dawson IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Kristen Broomall Sent: Tuesday, March 28, 2006 2:16 PM To: 'Jackie M O'Connor'; Joe Nocito Cc: histonet-bounces@lists.utsouthwestern.edu; histonet Subject: RE: [Histonet] Venting Part II I'll forward that to my very hardworking, intelligent, and diligent boyfriend who is a "Union Man". Perhaps dumbass is a better description for a single person who is not a good worker than to generalize that an entire group of people are incompetent. Kristen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jackie M O'Connor Sent: Tuesday, March 28, 2006 8:05 AM To: Joe Nocito Cc: histonet-bounces@lists.utsouthwestern.edu; histonet Subject: Re: [Histonet] Venting Part II I think you call that a Union Man. "Joe Nocito" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/27/2006 08:23 PM To: "histonet" cc: (bcc: Jackie M O'Connor/LAKE/GPRD/ABBOTT) Subject: [Histonet] Venting Part II Okay, as I said in my apology posting, I used "dumb ass" to describe the plumber who did not want to vent the flame cabinets. Please give me an alternative phrase for me to use because I don't understand the following events: 1. what do you call it when you put in a drench shower/eyewash station combo, but don't put in a drain? 2. what do you call it when you leave a 2" pipe open near the bottom of the shower/eyewash so that when you test the eyewash station, a rush of water soaks your shoes? 3. what do you call it when you put in a deionized water station, but don't know what a conductivity light is? So when you ask the plumber "how do you when to change the tank if there is no light", and the plumber just says "I don't know"?. 4. what do you call it when you blow a test ball through the drain system to test the drain system, yet forget to take the test ball out of the drain and water backs up into your new lab causing a huge flood in the lab? Then have to call in a competitor to help you fix it. 5. what do you call it when you try to light a gas water heater for 30 minutes, just to be told by some one else that there is no gas meter hooked up because you forgot to get the meter from the city? 6. what do you call it when you are soldering a pipe and activate the fire alarm system? I fell like Jeff Foxworthy "You might be a redneck if". Can some one please come up with an alternative phrase other than "dumb ass"? These events really happened this weekend in my new $1.5 million lab. I know, it sounds like fiction, but trust me, it's the real thing. So you see my dilemma. And for that one person who I offended in the past, I may be unprofessional in my language and conduct, but I do know MY job. Thanks for listening. In advance, I am sorry for the harsh language once again, but I can't come up with the words to accurately describe this "professional" plumber. Let the flaming begin (I'll just have to get another plumber to help put out the flames) Joe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lksell <@t> aol.com Wed Mar 29 08:38:45 2006 From: lksell <@t> aol.com (lksell@aol.com) Date: Wed Mar 29 08:39:01 2006 Subject: [Histonet] Her2 Fish Testing with Sakura Rapid Tissue Processor Message-ID: <8C821559E71DC62-5FC-7329@MBLK-R05.sysops.aol.com> Is there anyone out there who is doing Her2 Fish testing with breast core biopsies that have been processed on the Rapid Tissue Processor from Sakura. Leslie Sell Technical Coordinator Presbyterian Hospital Charlotte, NC From c.m.vanderloos <@t> amc.uva.nl Wed Mar 29 08:45:22 2006 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Wed Mar 29 08:45:57 2006 Subject: [Histonet] RE: mouse antibodies on mouse tissue Message-ID: <10745410a3b0.10a3b0107454@amc.uva.nl> Dear Claudia, The mouse monoclonal antibodies were never raised against mouse antigens at all. The point is that the original mice were immunized with antigens isolated mostly from human sources. Later it appeared that the antigens in question are very similar among several animal species. In fact so similar that the mouse monoclonal antibody also reacts with that same epitope in mouse tissue. Hope this helps, Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Wed, 29 Mar 2006 07:42:46 +1000 From: Claudia Freyer Subject: [Histonet] mouse antibodies on mouse tissue To: histonet@lists.utsouthwestern.edu Dear Histonetter, I am confused about the use of monoclonal antibodies produced in mice against endogenous proteins in mouse tissue. I thought an animal would not produce antibodies against its own proteins, since this would cause an autoimmune response. Would anybody know why mouse monoclonals detect epitopes on mouse proteins? Many thanks. Claudia Dr Claudia Freyer Prince Henry's Institute of Medical Research Victoria Australia From funderwood <@t> mcohio.org Wed Mar 29 08:46:22 2006 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Wed Mar 29 08:46:44 2006 Subject: [Histonet] spring ahead Message-ID: A reminder to save some of you a phone call. The VIP 5 does not automatically adjust to daylight savings, which is this upcoming weekend. From froyer <@t> bitstream.net Wed Mar 29 09:01:59 2006 From: froyer <@t> bitstream.net (Ford Royer) Date: Wed Mar 29 09:02:13 2006 Subject: [Histonet] Venting Part II In-Reply-To: <5D2189E74151CC42BEC02906BA8996322B91B0@exchsrv01.barrynet.barry.edu> Message-ID: <008301c65341$bb11bf80$6f01a80a@fords> I responded off list to this originally but I will add one comment to the list. Another reason that drains are not provided for emergency showers is that they (the shower and the drain) are not used on a regular basis... sometime never used in the life of the building. There is a danger that if the drains dry out that deadly sewer gas could back up into the room. This is true even with "waterless" trap drains. ~ Ford Ford M. Royer, MT(ASCP) Minnesota Medical Specialists, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 763-542-8725 phone 888-790-9686 toll free 763-546-4830 fax email web -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith, Allen Sent: Tuesday, March 28, 2006 3:33 PM To: Patti Loykasek Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Venting Part II The city isn't thinking very hard. 1/2 inch of water over the floor is going to be mopped up into a bucket. Guess where the bucket will be emptied? Emergency procedures are not the same as routine procedures. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Tuesday, March 28, 2006 1:27 PM To: Joe Nocito; histonet Subject: Re: [Histonet] Venting Part II Joe - I can truly sympathize with you since we moved into a new building about 3 yrs ago & certain contractors issues are still haunting us. Just one note - there might be a valid reason for not installing drains at the shower - you local regulations may not allow it. We don't have one because the city doesn't want any chemical contamination in their drainage system (save the salmon!). Good luck at your new location, enjoy it. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA Okay, as I said in my apology posting, I used "dumb ass" to describe the > plumber who did not want to vent the flame cabinets. Please give me an > alternative phrase for me to use because I don't understand the following > events: > 1. what do you call it when you put in a drench shower/eyewash station > combo, but don't put in a drain? > 2. what do you call it when you leave a 2" pipe open near the bottom of the > shower/eyewash so that when you test the eyewash station, a rush of water > soaks your shoes? > 3. what do you call it when you put in a deionized water station, but don't > know what a conductivity light is? So when you ask the plumber "how do you > when to change the tank if there is no light", and the plumber just says "I > don't know"?. > 4. what do you call it when you blow a test ball through the drain system to > test the drain system, yet forget to take the test ball out of the drain and > water backs up into your new lab causing a huge flood in the lab? Then have to > call in a competitor to help you fix it. > 5. what do you call it when you try to light a gas water heater for 30 > minutes, just to be told by some one else that there is no gas meter hooked up > because you forgot to get the meter from the city? > 6. what do you call it when you are soldering a pipe and activate the fire > alarm system? > > I fell like Jeff Foxworthy "You might be a redneck if". > > Can some one please come up with an alternative phrase other than "dumb ass"? > These events really happened this weekend in my new $1.5 million lab. I know, > it sounds like fiction, but trust me, it's the real thing. So you see my > dilemma. And for that one person who I offended in the past, I may be > unprofessional in my language and conduct, but I do know MY job. > > Thanks for listening. In advance, I am sorry for the harsh language once > again, but I can't come up with the words to accurately describe this > "professional" plumber. > > Let the flaming begin (I'll just have to get another plumber to help put out > the flames) > > Joe > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Wed Mar 29 09:06:50 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed Mar 29 09:07:15 2006 Subject: [Histonet] Re: Unions Message-ID: While my sense of humor may be somewhat skewed at times, it is never my intention to offend, irritate, annoy or otherwise displease anyone - period. For those hard working individuals who may belong to unions, my son-in-law the pipefitter included, I salute you. Please end this here. From vazquezr <@t> ohsu.edu Wed Mar 29 09:35:47 2006 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Wed Mar 29 09:36:14 2006 Subject: [Histonet] Venting Part II Message-ID: Glen, Thank you, thank you....I have seen this posting for the last two days or so and quite frankly, I am surprised that it has been allowed to go on as long as it has...I remember when acouple of the histo responders were making light of my name ROBYN and I light-heartedly responded, because I have a "great sense of humor" and you know what? I was cut off at the knees (sorry if I offended anybody with no legs) and was told that this should all stop with the commentary. Joe you do have valid complaints,, people lighten up. Now that I was able to vent, everyone have a nice day.... Robyn OHSU >>> "Dawson, Glen" 3/29/2006 5:52 AM >>> UHHHGG!!!! Kristen, Joe is making a general popular culture statement that is mixed with a bit of humor. Believe me, people are so easily offended on this list that the last thing we need is people to be getting offended on behalf of others as well. Political Correctness is the enemy of us all. When I see a word like dumbass on a post, quite honestly, it is a breath of fresh air. Everyone needs to thicken up their skins a bit before the rest of the world finds out just how WEAK we have all become. Glen Dawson IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Kristen Broomall Sent: Tuesday, March 28, 2006 2:16 PM To: 'Jackie M O'Connor'; Joe Nocito Cc: histonet-bounces@lists.utsouthwestern.edu; histonet Subject: RE: [Histonet] Venting Part II I'll forward that to my very hardworking, intelligent, and diligent boyfriend who is a "Union Man". Perhaps dumbass is a better description for a single person who is not a good worker than to generalize that an entire group of people are incompetent. Kristen -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jackie M O'Connor Sent: Tuesday, March 28, 2006 8:05 AM To: Joe Nocito Cc: histonet-bounces@lists.utsouthwestern.edu; histonet Subject: Re: [Histonet] Venting Part II I think you call that a Union Man. "Joe Nocito" Sent by: histonet-bounces@lists.utsouthwestern.edu 04/27/2006 08:23 PM To: "histonet" cc: (bcc: Jackie M O'Connor/LAKE/GPRD/ABBOTT) Subject: [Histonet] Venting Part II Okay, as I said in my apology posting, I used "dumb ass" to describe the plumber who did not want to vent the flame cabinets. Please give me an alternative phrase for me to use because I don't understand the following events: 1. what do you call it when you put in a drench shower/eyewash station combo, but don't put in a drain? 2. what do you call it when you leave a 2" pipe open near the bottom of the shower/eyewash so that when you test the eyewash station, a rush of water soaks your shoes? 3. what do you call it when you put in a deionized water station, but don't know what a conductivity light is? So when you ask the plumber "how do you when to change the tank if there is no light", and the plumber just says "I don't know"?. 4. what do you call it when you blow a test ball through the drain system to test the drain system, yet forget to take the test ball out of the drain and water backs up into your new lab causing a huge flood in the lab? Then have to call in a competitor to help you fix it. 5. what do you call it when you try to light a gas water heater for 30 minutes, just to be told by some one else that there is no gas meter hooked up because you forgot to get the meter from the city? 6. what do you call it when you are soldering a pipe and activate the fire alarm system? I fell like Jeff Foxworthy "You might be a redneck if". Can some one please come up with an alternative phrase other than "dumb ass"? These events really happened this weekend in my new $1.5 million lab. I know, it sounds like fiction, but trust me, it's the real thing. So you see my dilemma. And for that one person who I offended in the past, I may be unprofessional in my language and conduct, but I do know MY job. Thanks for listening. In advance, I am sorry for the harsh language once again, but I can't come up with the words to accurately describe this "professional" plumber. Let the flaming begin (I'll just have to get another plumber to help put out the flames) Joe _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Amanda.Garcia <@t> TriadHospitals.com Wed Mar 29 09:44:38 2006 From: Amanda.Garcia <@t> TriadHospitals.com (Garcia, Amanda) Date: Wed Mar 29 09:44:47 2006 Subject: [Histonet] pams Message-ID: <8B63039C9DF4554C8FDBF31235F0E14801206BD0@CPRTEVS02.triadhospitals.net> Could someone please forward a procedure for mircrowave pams procedure. thanks > Amanda (Amy) Garcia > Histology/Pathology > College Station Medical Center > (979) 680-5372 office > (979) 696-5446 fax > *Mailto:amanda.garcia@triadhospitals.com > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the e-mail or any > of its attachments, please be advised that you have received this > e-mail in error and that any use, dissemination, distribution, > forwarding, printing, or copying of this e-mail or any attached files > is strictly prohibited. If you have received this e-mail in error, > please immediately purge it and all attachments and notify the sender > by reply e-mail or contact the sender at the number listed. > > > From LRaff <@t> lab.uropartners.com Wed Mar 29 09:47:46 2006 From: LRaff <@t> lab.uropartners.com (Lester Raff) Date: Wed Mar 29 09:47:49 2006 Subject: [Histonet] Technologist for Cytology and Fish Message-ID: <5DA1CA5D0B98A84985B545A24423B822A791@UPLAB01.uplab.local> Hello all: We are a small private single specialty lab, and will be branching off into cytology and FISH. Can anyone give me an idea as to the salary range for a lead tech in those areas? I would also like to hear from interested candidates. We are in the Chicago metro area. Thanks, Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory ph: 708-486-0076 fax: 708-486-0080 From aep10 <@t> cornell.edu Wed Mar 29 09:52:59 2006 From: aep10 <@t> cornell.edu (Anna Elisse Beaudin) Date: Wed Mar 29 09:53:02 2006 Subject: [Histonet] IHC double-labeling -- same secondary? Message-ID: <1841.128.253.96.73.1143647579.squirrel@webmail.cornell.edu> Hi all, I am performing double labeling IHC on mouse brain tissue with two primaries from different species, one raised in rat, the other in goat. I was wondering if it was kosher (or even preferred) to use both a donkey anti-rat secondary and a donkey-anti goat secondary. if so, can I incubate with both together as a cocktail? my donkey anti-rat is flourophore conjugated, and I was planning to use biotinylated donkey anti-goat followed with a flourophore-conjugated streptavidin. any suggestions/comments would be greatly appreciated. Thanks in advance, Anna Beaudin Division of Nutritional Sciences Cornell University From micro <@t> superlink.net Wed Mar 29 10:15:52 2006 From: micro <@t> superlink.net (Markus F. Meyenhofer) Date: Wed Mar 29 10:16:03 2006 Subject: [Histonet] Re: Unions References: Message-ID: <05c001c6534c$0f0259b0$7a893cd1@DJ4VDH31> Yeah! Get back to work! ----- Original Message ----- From: "Jackie M O'Connor" To: Sent: Wednesday, March 29, 2006 10:06 AM Subject: [Histonet] Re: Unions > While my sense of humor may be somewhat skewed at times, it is never my > intention to offend, irritate, annoy or otherwise displease anyone - > period. > For those hard working individuals who may belong to unions, my son-in-law > the pipefitter included, I salute you. Please end this here. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From liz <@t> premierlab.com Wed Mar 29 10:35:27 2006 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Mar 29 10:35:36 2006 Subject: [Histonet] porcine alpha smooth muscle actina and cytokeratin 7 Message-ID: <000501c6534e$c9126be0$0300a8c0@Chlipala> Hello all I was wondering if anyone new of an alpha smooth muslce actin and cytokeratin 7 antibody that will react with porcine cells only and not react with mouse or dog. I'm guessing that there might not be any, but I though I would post this anyway. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 From Karen.Heckford <@t> CHW.edu Wed Mar 29 11:44:08 2006 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Wed Mar 29 11:48:26 2006 Subject: [Histonet] Dermatology Dictation Message-ID: Hi Everyone, I am looking for a good publication on how to gross and dictate human dermatology. Any help would much appreciated. Thanks, Karen Heckford HT (ASCP) CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 1-415-668-1000 Ext. 6167 kheckfor@chw.edu From koellinr <@t> amgen.com Wed Mar 29 11:53:11 2006 From: koellinr <@t> amgen.com (Koelling, Ray) Date: Wed Mar 29 11:55:01 2006 Subject: [Histonet] mouse antibodies on mouse tissue Message-ID: <16834C6DFFA6004C88DE4507FB8AE54403947C76@wa-mb4-sea.amgen.com> Claudia, the other responders I think have a different interpretation of what I think you are asking since you asked about endogenous mouse proteins and nothing about human. Indeed there are mouse antibodies directed specifically against mouse proteins. We use them all the time. They are available commercially from many sources. Cell proteins from a strain of mouse injected into another mouse of same strain (syngeneic meaning identical) would be tolerated and it would be tough to make antibodies in that fashion. Actually you can but it is beyond this discussion. Cell proteins from a strain of mouse injected into a mouse of a different strain (allogenic meaning same species but different at some genetic loci) will certainly create antibodies. An example I'm looking at is an antibody to I-A (of d). It is a mouse monoclonal. The immunogen was mouse cells of a different strain. So the antibody (a mouse monoclonal) reacts to MHC class II alloantigen of certain haplotypes but not another. There are many, many antibodies like this. The short story is that mouse monoclonal antibodies can be made against mouse protein targets and there are actually other ways to do this than is addressed above. I think this is what you were asking but the others may have addressed the question more correctly. Ray Raymond Koelling Pathology Research Scientist Amgen Corp. Seattle, WA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Claudia Freyer Sent: Tuesday, March 28, 2006 1:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] mouse antibodies on mouse tissue Dear Histonetter, I am confused about the use of monoclonal antibodies produced in mice against endogenous proteins in mouse tissue. I thought an animal would not produce antibodies against its own proteins, since this would cause an autoimmune response. Would anybody know why mouse monoclonals detect epitopes on mouse proteins? Many thanks. Claudia Dr Claudia Freyer Prince Henry's Institute of Medical Research Victoria Australia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From asmith <@t> mail.barry.edu Wed Mar 29 11:56:12 2006 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Wed Mar 29 11:56:18 2006 Subject: [Histonet] Venting Part II Message-ID: <5D2189E74151CC42BEC02906BA8996322B91B1@exchsrv01.barrynet.barry.edu> I stand corrected, at least for facilities where the shower is separate from the eye wash station. Our shower and eye wash station use a common drain, and we flush the rust out of the eye wash station once a week. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ford Royer Sent: Wednesday, March 29, 2006 10:02 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Venting Part II I responded off list to this originally but I will add one comment to the list. Another reason that drains are not provided for emergency showers is that they (the shower and the drain) are not used on a regular basis... sometime never used in the life of the building. There is a danger that if the drains dry out that deadly sewer gas could back up into the room. This is true even with "waterless" trap drains. ~ Ford Ford M. Royer, MT(ASCP) Minnesota Medical Specialists, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 763-542-8725 phone 888-790-9686 toll free 763-546-4830 fax email web -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Smith, Allen Sent: Tuesday, March 28, 2006 3:33 PM To: Patti Loykasek Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Venting Part II The city isn't thinking very hard. 1/2 inch of water over the floor is going to be mopped up into a bucket. Guess where the bucket will be emptied? Emergency procedures are not the same as routine procedures. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Tuesday, March 28, 2006 1:27 PM To: Joe Nocito; histonet Subject: Re: [Histonet] Venting Part II Joe - I can truly sympathize with you since we moved into a new building about 3 yrs ago & certain contractors issues are still haunting us. Just one note - there might be a valid reason for not installing drains at the shower - you local regulations may not allow it. We don't have one because the city doesn't want any chemical contamination in their drainage system (save the salmon!). Good luck at your new location, enjoy it. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA Okay, as I said in my apology posting, I used "dumb ass" to describe the > plumber who did not want to vent the flame cabinets. Please give me an > alternative phrase for me to use because I don't understand the following > events: > 1. what do you call it when you put in a drench shower/eyewash station > combo, but don't put in a drain? > 2. what do you call it when you leave a 2" pipe open near the bottom of the > shower/eyewash so that when you test the eyewash station, a rush of water > soaks your shoes? > 3. what do you call it when you put in a deionized water station, but don't > know what a conductivity light is? So when you ask the plumber "how do you > when to change the tank if there is no light", and the plumber just says "I > don't know"?. > 4. what do you call it when you blow a test ball through the drain system to > test the drain system, yet forget to take the test ball out of the drain and > water backs up into your new lab causing a huge flood in the lab? Then have to > call in a competitor to help you fix it. > 5. what do you call it when you try to light a gas water heater for 30 > minutes, just to be told by some one else that there is no gas meter hooked up > because you forgot to get the meter from the city? > 6. what do you call it when you are soldering a pipe and activate the fire > alarm system? > > I fell like Jeff Foxworthy "You might be a redneck if". > > Can some one please come up with an alternative phrase other than "dumb ass"? > These events really happened this weekend in my new $1.5 million lab. I know, > it sounds like fiction, but trust me, it's the real thing. So you see my > dilemma. And for that one person who I offended in the past, I may be > unprofessional in my language and conduct, but I do know MY job. > > Thanks for listening. In advance, I am sorry for the harsh language once > again, but I can't come up with the words to accurately describe this > "professional" plumber. > > Let the flaming begin (I'll just have to get another plumber to help put out > the flames) > > Joe > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From jason.burrill <@t> crl.com Wed Mar 29 12:25:00 2006 From: jason.burrill <@t> crl.com (jason.burrill@crl.com) Date: Wed Mar 29 12:25:20 2006 Subject: [Histonet] Health and Safety Guidelines for the Lab by L Montgomery needed Message-ID: Hello all, I am in the process of taking the SLS (Specialist in Laboratory Safety) certification from ASCP and can not locate a new or used text of "Health and Safety Guidelines for the Laboratory" by Lynn Montgomery. I have searched the internet, Amazon and contacted ASCP with no luck (out of print), if anyone has any suggestions on where else I can look I would really appreciate it. Thanks in advance for your help. Jason Jason D. Burrill Manager, Histology Charles River Laboratories 251 Ballardvale Street Wilmington, MA 01887 ph: 978-658-6000 ext 1652 fax: 978-988-8793 E-mail: jason.burrill@crl.com From sbreeden <@t> nmda.nmsu.edu Wed Mar 29 12:26:06 2006 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Mar 29 12:26:11 2006 Subject: [Histonet] Dagnabbit! Message-ID: Rats and mice! Another good Friday Hour of Flaming (a.k.a. Fuming) subject I missed! And I do so enjoy a good verbal scuffle in the morning. Could y'all please save these pseudo-histologic fumings for the Appointed Hour, for heaven's sake? Sally Breeden, HT(ASCP) New Mexico Department of Agriculture Veterinary Diagnostic Services POBox 4700 Albuquerque, NM 87196 (505)841-2576 (505)841-2518 FAX From DavidH <@t> marketlabinc.com Wed Mar 29 12:35:24 2006 From: DavidH <@t> marketlabinc.com (David Haagsma) Date: Wed Mar 29 12:35:34 2006 Subject: [Histonet] Health and Safety Guidelines for the Lab by L Montgomeryneeded Message-ID: <19E3602A16438E48B51A4250CA04B5F6591564@exchange.marketlab.com> Jason, Check this link to find it - This is a specialty book dealer in Natick Mass. http://www.labsafety.org/store/enter.html?lang=en-us&target=d7_02.html Hope this helps. Dave Haagsma Research Market Manager MarketLab Inc. 6850 Southbelt Dr Caledonia MI 49316 616-871-3247 800-237-3604 Fax 616-656-2475 Unique and Hard to Find Products for your Laboratory http://www.marketlabinc.com/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jason.burrill@crl.com Sent: Wednesday, March 29, 2006 1:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Health and Safety Guidelines for the Lab by L Montgomeryneeded Hello all, I am in the process of taking the SLS (Specialist in Laboratory Safety) certification from ASCP and can not locate a new or used text of "Health and Safety Guidelines for the Laboratory" by Lynn Montgomery. I have searched the internet, Amazon and contacted ASCP with no luck (out of print), if anyone has any suggestions on where else I can look I would really appreciate it. Thanks in advance for your help. Jason Jason D. Burrill Manager, Histology Charles River Laboratories 251 Ballardvale Street Wilmington, MA 01887 ph: 978-658-6000 ext 1652 fax: 978-988-8793 E-mail: jason.burrill@crl.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wfubmc.edu Wed Mar 29 12:52:16 2006 From: mward <@t> wfubmc.edu (Martha Ward) Date: Wed Mar 29 12:53:40 2006 Subject: [Histonet] Glycophorin A Message-ID: <61135F0455D33347B5AAE209B903A304132A3AD4@EXCHVS2.medctr.ad.wfubmc.edu> One of our Pathologists have asked about working this antibody up to add to our menu. It is available from several vendors but I wanted to check and see which is most commonly used. We will be using it on the Ventana NexEs. Thanks in advance for your help with this. Martha Ward, MT ASCP (QIHC) Wake Forest University Baptist Medical Center From Janet.Bonner <@t> FLHOSP.ORG Wed Mar 29 12:53:08 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Wed Mar 29 12:55:00 2006 Subject: [Histonet] spring ahead References: Message-ID: Thank you Fred! I called Sakura and veified that the E300 does not change automatically either as some states do not observe Daylight Savings Time (like Arizona) ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Fred Underwood Sent: Wed 3/29/2006 9:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] spring ahead A reminder to save some of you a phone call. The VIP 5 does not automatically adjust to daylight savings, which is this upcoming weekend. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From settembr <@t> umdnj.edu Wed Mar 29 13:02:38 2006 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Wed Mar 29 13:03:55 2006 Subject: [Histonet] Glycophorin A Message-ID: Hello Martha, I use Glycophorin A from Dako cat# M0819 Dana Settembre UMDNJ - University Hospital Newark, NJ >>> Martha Ward 03/29/06 1:52 PM >>> One of our Pathologists have asked about working this antibody up to add to our menu. It is available from several vendors but I wanted to check and see which is most commonly used. We will be using it on the Ventana NexEs. Thanks in advance for your help with this. Martha Ward, MT ASCP (QIHC) Wake Forest University Baptist Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jeannette.Mitchell <@t> vtmednet.org Wed Mar 29 13:15:59 2006 From: Jeannette.Mitchell <@t> vtmednet.org (Mitchell, Jeannette M.) Date: Wed Mar 29 13:16:21 2006 Subject: [Histonet] (no subject) Message-ID: Hello everyone, One of the pathologist at Fletcher Allen ask me to post the following question: Is there an established protocol for the number of levels to be done on cervical biopsies and what documentation is this protocol based on ? i.e. study, journal article ? Jeannette Mitchell FAHC Burlington,VT Confidentiality Notice: This message, and any attachments, may contain information that is confidential, privileged, and/or protected from disclosure under state and federal laws that deal with the privacy and security of medical information. If you received this message in error or through inappropriate means, please reply to this message to notify the Sender that the message was received by you in error, and then permanently delete this message from all storage media, without forwarding or retaining a copy. From Jeannette.Mitchell <@t> vtmednet.org Wed Mar 29 13:17:40 2006 From: Jeannette.Mitchell <@t> vtmednet.org (Mitchell, Jeannette M.) Date: Wed Mar 29 13:17:44 2006 Subject: [Histonet] job opening Message-ID: Histotechnician Fletcher Allen Health Care, a level I trauma center with a level III NICU is a 500-bed teaching hospital affiliated with the University of Vermont. Fletcher Allen Health Care is the third largest employer in the state of Vermont, and we are seeking a full-time Histotechnician to join our team. Our Pathology department processes over 30,000 surgical cases and 500 autopsies a year. Histotechs perform routine special stains, automated Immunohistochemistry, and muscle histochemistries. The ideal candidate will have an Associate or Bachelor's degree and HT and/or HTL certification (or eligible). Off shifts may be required. Our employees enjoy a comprehensive benefit package including medical, dental, vision, retirement, life insurance and a paid time off. We also offer a generous relocation package that includes moving assistance and temporary housing! To apply use our on-line resume builder at www.fletcherallen.org or email your cover letter and resume to: fahcjobs@vtmednet.org (no attachment) or mail to: Human Resources, FAHC, 111 Colchester Ave., Burlington, VT 05401. Scannable resumes should be on white paper with standard fonts, no bold, underline or italics. Please list posting number 05-2419 when applying. EOE Confidentiality Notice: This message, and any attachments, may contain information that is confidential, privileged, and/or protected from disclosure under state and federal laws that deal with the privacy and security of medical information. If you received this message in error or through inappropriate means, please reply to this message to notify the Sender that the message was received by you in error, and then permanently delete this message from all storage media, without forwarding or retaining a copy. From ROrr <@t> enh.org Wed Mar 29 13:18:30 2006 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Wed Mar 29 13:18:37 2006 Subject: [Histonet] RE: Histonet Digest, Vol 28, Issue 47 Message-ID: I can start whining about Med Techs again... Would that help change the subject? Becky Orr CLA,HT(ASCP) IHC Lead Evanston Northwestern Healthcare 847-570-2771 From c.m.vanderloos <@t> amc.uva.nl Wed Mar 29 13:29:43 2006 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Wed Mar 29 13:30:52 2006 Subject: [Histonet] RE: IHC double-labeling -- same secondary? Message-ID: Hi Anna Elisse, To my opinion there is a strong preference of using second step reagents of the same host. That makes it very safe to mix them in a cocktail (time saving!) without any chance of unexpected cross-reactions. In your case you could mix donkey anti-rat/FLUO + donkey anti-goat/biotin. Don't forget that you could mix your primaries in a cocktail as well! Good luck, Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Wed, 29 Mar 2006 10:52:59 -0500 (EST) From: "Anna Elisse Beaudin" Subject: [Histonet] IHC double-labeling -- same secondary? To: Histonet@lists.utsouthwestern.edu Hi all, I am performing double labeling IHC on mouse brain tissue with two primaries from different species, one raised in rat, the other in goat. I was wondering if it was kosher (or even preferred) to use both a donkey anti-rat secondary and a donkey-anti goat secondary. if so, can I incubate with both together as a cocktail? my donkey anti-rat is flourophore conjugated, and I was planning to use biotinylated donkey anti-goat followed with a flourophore-conjugated streptavidin. any suggestions/comments would be greatly appreciated. Thanks in advance, Anna Beaudin Division of Nutritional Sciences Cornell University From RohrT <@t> nyackhospital.org Wed Mar 29 13:39:25 2006 From: RohrT <@t> nyackhospital.org (Theresa Rohr) Date: Wed Mar 29 13:41:55 2006 Subject: [Histonet] CD10 Message-ID: Hello, I was wondering if anyone has any suggestions regarding CD10. I have the Dako Autostainer and use the Envision+ system and a Steamer for Antigen Retrieval. Since Dako only has an antibody for use on Frozen sections, I am searching for one for FFPE tissue. My Dako Rep actually suggested Novocastro as having a great antibody. Does anyone out there agree/disagree??? At what dilution???? What I already researched looks like this is not a cheap antibody in terms of dillution. I would appreciate any help. Thanks so much, Theresa Rohr, Nyack Hospital rohrt@nyackhospital.org Theresa Rohr, BA, HT(ASCP) Section Head, Histology Nyack Hospital 160 North Midland Avenue Nyack, New York 10960 phone 845-348-2276 fax 845-348-8430 Nyack Hospital is required by Law to protect the privacy of health information under HIPAA Rules and Regulations Part 11, Article 45 CFR, Parts 160-164. This email, including attachments, is intended for the exclusive use of the person or the entity to which it is addressed. It may contain confidential or legally protected information. The authorized recipient is prohibited from disclosing this information to any other party and is required to destroy this information after its stated need has been fulfilled. If the recipient or reader of this email is not the intended recipient or her/his authorized agent, the reader is hereby notified that any dissemination, distribution or copying of this email is prohibited. If you believe that you have received this email in error, please advise the sender immediately by reply email and then delete this email immediately. From ROrr <@t> enh.org Wed Mar 29 13:43:22 2006 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Wed Mar 29 13:47:41 2006 Subject: [Histonet] RE: Double staining Message-ID: Anna, I am very interested in this. I am not a chemist, so I would welcome more educated predictions. But theoretically, this secondary cocktail MIGHT work. My exposure to this is very limited and is based on my experience with the Biocare doublestain secondary cocktails. You would need to get more information on the actual chemistry of the secondary components...would the flourophore "smother" out the other secondary? I would question how the biotinylated component would cross react with the flourophore...I have no direct experience to this. Then there's the dilution of each when they are placed together... I see the main question is how well the flourophore can cohabitate with your biotinylated secondary Have you tried each stain separately? Then doing the double stain the "traditional" way? If you could get a base line, that might help to see what happens when you start making the voodoo brew. Let me know how this goes! Becky Orr CLA,HT(ASCP) IHC Lead Evanston Northwestern Healthcare 847-570-2771 From settembr <@t> umdnj.edu Wed Mar 29 13:47:27 2006 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Wed Mar 29 13:48:13 2006 Subject: [Histonet] CD10 Message-ID: Hello Theresa, I use CD10 from NovoCastra at a 1:40 dilution. I use it with Trilogy in the steamer from Cell Marque as retrieval. I also use Env+ and the Dako Autostainer. Good Luck Dana Settembre University Hospital - UMDNJ Newark, NJ >>> Theresa Rohr 03/29/06 2:39 PM >>> Hello, I was wondering if anyone has any suggestions regarding CD10. I have the Dako Autostainer and use the Envision+ system and a Steamer for Antigen Retrieval. Since Dako only has an antibody for use on Frozen sections, I am searching for one for FFPE tissue. My Dako Rep actually suggested Novocastro as having a great antibody. Does anyone out there agree/disagree??? At what dilution???? What I already researched looks like this is not a cheap antibody in terms of dillution. I would appreciate any help. Thanks so much, Theresa Rohr, Nyack Hospital rohrt@nyackhospital.org Theresa Rohr, BA, HT(ASCP) Section Head, Histology Nyack Hospital 160 North Midland Avenue Nyack, New York 10960 phone 845-348-2276 fax 845-348-8430 Nyack Hospital is required by Law to protect the privacy of health information under HIPAA Rules and Regulations Part 11, Article 45 CFR, Parts 160-164. This email, including attachments, is intended for the exclusive use of the person or the entity to which it is addressed. It may contain confidential or legally protected information. The authorized recipient is prohibited from disclosing this information to any other party and is required to destroy this information after its stated need has been fulfilled. If the recipient or reader of this email is not the intended recipient or her/his authorized agent, the reader is hereby notified that any dissemination, distribution or copying of this email is prohibited. If you believe that you have received this email in error, please advise the sender immediately by reply email and then delete this email immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Wed Mar 29 14:35:26 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Mar 29 14:35:40 2006 Subject: Double IFA staining per Re: [Histonet] RE: Double staining In-Reply-To: References: Message-ID: <6.0.0.22.1.20060329125826.01b3d540@gemini.msu.montana.edu> At 12:43 PM 3/29/2006, you wrote: >Anna, >I am very interested in this. I am not a chemist, so I would welcome more >educated predictions. >But theoretically, this secondary cocktail MIGHT work. My exposure to this >is very limited and is based on my experience with the Biocare doublestain >secondary cocktails. You would need to get more information on the actual >chemistry of the >secondary components...would the flourophore "smother" out the other >secondary? No, in fact you can mix two secondaries each conjugated to different fluorophores if you wanted to. >I would question how the biotinylated component would cross react with the >flourophore Bioinylated secondary cannot react with a fluorophore - it needs the next reagent, a Strepavidin-fluorophore (per the first question asked). >...I have no direct experience to this. >Then there's the dilution of each when they are placed together. We merely make up one antibody and dilute the second in correct volume of the first primary dilution to have the correct concentration of our second primary. The diluent would be the same since the host of these two secondaries is identical (we would probably use 5% donkey serum). Not a difficult task, and a little math. >I see the main question is how well the flourophore can cohabitate with >your biotinylated secondary No reason not to, however the main thing is to protect this step from light during incubation to prevent photobleaching of the fluorophore. >Have you tried each stain separately? An excellent suggestion and the best way to determine optimal results with EACH antibody and then combine in a cocktail for later staining (faster, to be sure!) >Then doing the double stain the "traditional" way? What do you mean by "traditional" way, a sequential staining? Please elaborate with a protocol? If you have not already done so, I strongly suggest you access Chris van der Loos's Immunoenzyme Multiple Staining Methods and see how cocktails of primaries and secondaries, etc can be cleverly used efficiently and without problems. He is also presenting an all day indepth workshop on multiple staining at NSH in Phoenix this September. >If you could get a base line, that might help to see what happens when you >start making the voodoo brew. We love our voo doo brews, see the following example and if my second primary antibody had been purified, I would have happily mixed the fluorophore conjugated secondary with a biotinylated secondary, and then the SA-Alexa dye. Goat antiGFP in a cocktail with biotinylated CD11c (dendritic cell marker) rat antiMouse Donkey anti Goat-FITC followed by Strepavidin-Alexa 555 in a diluent that does NOT contain any normal serums - we cannot cocktail the Dk antiGoat FITC which we would dilute in donkey serum since SA can bind to any endogenous biotin in the normal serum (a warning given by Molecular Probes) People doing FACS work are always making cocktails of their antibodies conjugated to fluorophores, sometime more than two or three without problems. The only problem I ever had with mouse CD marker for an IFA stain was using a directly CD4-FITC - there was no fluorescence visible. There is some physical barrier here, a spatial or stoichometric (sp?) problem/configuration that did not allow the FITC to glow. It was sequestered in some way. In our lab, we never use kits for double IFA work, it is all inhouse dilutions of stock/concentrated of primaries, secondaries, and Strepavidin Alexa dyes, something very typical with research labs at times. The biggest problem with some of the kit secondary cocktails i.e. multilinks is that you don't end up with one of the links (mouse?) becoming a problem with background/unwanted cross reactions - something that would affect our mouse tissue work. But it is sooo much fun Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 From rjbuesa <@t> yahoo.com Wed Mar 29 14:39:54 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 29 14:39:59 2006 Subject: [Histonet] CD10 In-Reply-To: Message-ID: <20060329203954.50304.qmail@web61225.mail.yahoo.com> Theresa: I used CD10 from Novocastra at a dilution of 1:5 with pH6 HIER I know of a lab in the UK that dilutes it 1:10 (and seem to work OK, I have seen their slides). Hope this will help! Ren? J. Theresa Rohr wrote: Hello, I was wondering if anyone has any suggestions regarding CD10. I have the Dako Autostainer and use the Envision+ system and a Steamer for Antigen Retrieval. Since Dako only has an antibody for use on Frozen sections, I am searching for one for FFPE tissue. My Dako Rep actually suggested Novocastro as having a great antibody. Does anyone out there agree/disagree??? At what dilution???? What I already researched looks like this is not a cheap antibody in terms of dillution. I would appreciate any help. Thanks so much, Theresa Rohr, Nyack Hospital rohrt@nyackhospital.org Theresa Rohr, BA, HT(ASCP) Section Head, Histology Nyack Hospital 160 North Midland Avenue Nyack, New York 10960 phone 845-348-2276 fax 845-348-8430 Nyack Hospital is required by Law to protect the privacy of health information under HIPAA Rules and Regulations Part 11, Article 45 CFR, Parts 160-164. This email, including attachments, is intended for the exclusive use of the person or the entity to which it is addressed. It may contain confidential or legally protected information. The authorized recipient is prohibited from disclosing this information to any other party and is required to destroy this information after its stated need has been fulfilled. If the recipient or reader of this email is not the intended recipient or her/his authorized agent, the reader is hereby notified that any dissemination, distribution or copying of this email is prohibited. If you believe that you have received this email in error, please advise the sender immediately by reply email and then delete this email immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. PC-to-Phone calls for ridiculously low rates. From ploykasek <@t> phenopath.com Wed Mar 29 14:49:46 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Wed Mar 29 14:50:11 2006 Subject: [Histonet] CD10 In-Reply-To: Message-ID: Hi Theresa. We use the Novocastro antibody at 1:100 with a heat citrate pretreatment, and Env+ detection done on the Dako Autostainer. For this antibody our pretreatment is done in a microwave pressure cooker, perhaps that is why our titer is more dilute than some others have reported. It is a nice antibody, but not cheap. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Hello, > > I was wondering if anyone has any suggestions regarding CD10. I have the Dako > Autostainer and use the Envision+ system and a Steamer for Antigen Retrieval. > > Since Dako only has an antibody for use on Frozen sections, I am searching for > one for FFPE tissue. My Dako Rep actually suggested Novocastro as having a > great antibody. > > Does anyone out there agree/disagree??? > At what dilution???? > > What I already researched looks like this is not a cheap antibody in terms of > dillution. > > I would appreciate any help. > > Thanks so much, > Theresa Rohr, Nyack Hospital > rohrt@nyackhospital.org > > Theresa Rohr, BA, HT(ASCP) > Section Head, Histology > Nyack Hospital > 160 North Midland Avenue > Nyack, New York 10960 > phone 845-348-2276 > fax 845-348-8430 > > Nyack Hospital is required by Law to protect the privacy of health information > under HIPAA Rules and Regulations Part 11, Article 45 CFR, Parts 160-164. This > email, including attachments, is intended for the exclusive use of the person > or the entity to which it is addressed. It may contain confidential or legally > protected information. The authorized recipient is prohibited from disclosing > this information to any other party and is required to destroy this > information after its stated need has been fulfilled. If the recipient or > reader of this email is not the intended recipient or her/his authorized > agent, the reader is hereby notified that any dissemination, distribution or > copying of this email is prohibited. If you believe that you have received > this email in error, please advise the sender immediately by reply email and > then delete this email immediately. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From Rcartun <@t> harthosp.org Wed Mar 29 14:56:20 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Mar 29 15:04:20 2006 Subject: [Histonet] Rapid Decal Message-ID: Is anyone using "Rapid Decal" for bone marrow core biopsies? If so, how do you like it (advantages vs. disadvantages)? Where do you get it? Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From JPaulB42 <@t> aol.com Wed Mar 29 15:32:55 2006 From: JPaulB42 <@t> aol.com (JPaulB42@aol.com) Date: Wed Mar 29 15:33:11 2006 Subject: [Histonet] Tissue hardening Message-ID: <2bb.7a5b275.315c5707@aol.com> Greeting all, My next question is on tissue hardening. We are using the Milestone RHS-1 microwave processor. There is a program for tissue hardening but no time frame. Any suggestions on how long to what volume of tissue to use? And how about which to use, either 10% formalin at 50*C or PBS at 65*C? I am just starting this project so please bare with me as I will probably ask many questions as I go along. Thank you for all your assistance. Jim Bradford From Rcartun <@t> harthosp.org Wed Mar 29 15:38:40 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Mar 29 15:43:08 2006 Subject: [Histonet] CD10 Message-ID: A 1:5 (or 1:10) dilution of an antibody doesn't sound very practical in terms of cost per slide. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> Rene J Buesa 03/29/06 03:39PM >>> Theresa: I used CD10 from Novocastra at a dilution of 1:5 with pH6 HIER I know of a lab in the UK that dilutes it 1:10 (and seem to work OK, I have seen their slides). Hope this will help! Ren? J. Theresa Rohr wrote: Hello, I was wondering if anyone has any suggestions regarding CD10. I have the Dako Autostainer and use the Envision+ system and a Steamer for Antigen Retrieval. Since Dako only has an antibody for use on Frozen sections, I am searching for one for FFPE tissue. My Dako Rep actually suggested Novocastro as having a great antibody. Does anyone out there agree/disagree??? At what dilution???? What I already researched looks like this is not a cheap antibody in terms of dillution. I would appreciate any help. Thanks so much, Theresa Rohr, Nyack Hospital rohrt@nyackhospital.org Theresa Rohr, BA, HT(ASCP) Section Head, Histology Nyack Hospital 160 North Midland Avenue Nyack, New York 10960 phone 845-348-2276 fax 845-348-8430 Nyack Hospital is required by Law to protect the privacy of health information under HIPAA Rules and Regulations Part 11, Article 45 CFR, Parts 160-164. This email, including attachments, is intended for the exclusive use of the person or the entity to which it is addressed. It may contain confidential or legally protected information. The authorized recipient is prohibited from disclosing this information to any other party and is required to destroy this information after its stated need has been fulfilled. If the recipient or reader of this email is not the intended recipient or her/his authorized agent, the reader is hereby notified that any dissemination, distribution or copying of this email is prohibited. If you believe that you have received this email in error, please advise the sender immediately by reply email and then delete this email immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. PC-to-Phone calls for ridiculously low rates. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Mar 29 15:43:38 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Mar 29 15:43:45 2006 Subject: [Histonet] Rapid Decal In-Reply-To: Message-ID: <20060329214338.29943.qmail@web61219.mail.yahoo.com> Richard: For core bone marrow core biopsies I always used EDTA pH7 Any "rapid decalcifyer" was counterproductive with regards to staining quality. Ren? J. Richard Cartun wrote: Is anyone using "Rapid Decal" for bone marrow core biopsies? If so, how do you like it (advantages vs. disadvantages)? Where do you get it? Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- New Yahoo! Messenger with Voice. Call regular phones from your PC for low, low rates. From anh2006 <@t> med.cornell.edu Wed Mar 29 15:44:44 2006 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Wed Mar 29 15:44:20 2006 Subject: [Histonet] RE: Double staining In-Reply-To: References: Message-ID: Hi Anna, Your experiment will work! We do this all the time here with very nice results and it also sounds like you know what you are talking about. I suggest you check to make sure the secondaries are highly cross adsorbed and if not, buy ones which are (Jackson ImmunoResearch sells awesome secondaries). I also strongly suggest you run each independently to make sure everything is in order. I normally do this alongside my double stains each time. Also as Chris suggested you can even incubate your primaries together making your experiment even additionally streamlined. And of course remember to block appropriately. Good luck, Andrea >Anna, >I am very interested in this. >I am not a chemist, so I would welcome more educated predictions. >But theoretically, this secondary cocktail MIGHT work. My exposure to >this is very limited and is based on my experience with the Biocare >doublestain secondary cocktails. >You would need to get more information on the actual chemistry of the >secondary components...would the flourophore "smother" out the other >secondary? I would question how the biotinylated component would cross >react with the flourophore...I have no direct experience to this. >Then there's the dilution of each when they are placed together... >I see the main question is how well the flourophore can cohabitate with >your biotinylated secondary > >Have you tried each stain separately? >Then doing the double stain the "traditional" way? > >If you could get a base line, that might help to see what happens when >you start making the voodoo brew. > >Let me know how this goes! > > Becky Orr CLA,HT(ASCP) >IHC Lead >Evanston Northwestern Healthcare >847-570-2771 > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- From AnthonyH <@t> chw.edu.au Wed Mar 29 16:02:35 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Mar 29 16:02:51 2006 Subject: [Histonet] CD10 Message-ID: We use Novocastra's NCL-CD10-270 1/100 dilution with EDTA retrieval (20min) on the Bond Max Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, 30 March 2006 7:40 AM To: Theresa Rohr; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] CD10 Theresa: I used CD10 from Novocastra at a dilution of 1:5 with pH6 HIER I know of a lab in the UK that dilutes it 1:10 (and seem to work OK, I have seen their slides). Hope this will help! Ren? J. Theresa Rohr wrote: Hello, I was wondering if anyone has any suggestions regarding CD10. I have the Dako Autostainer and use the Envision+ system and a Steamer for Antigen Retrieval. Since Dako only has an antibody for use on Frozen sections, I am searching for one for FFPE tissue. My Dako Rep actually suggested Novocastro as having a great antibody. Does anyone out there agree/disagree??? At what dilution???? What I already researched looks like this is not a cheap antibody in terms of dillution. I would appreciate any help. Thanks so much, Theresa Rohr, Nyack Hospital rohrt@nyackhospital.org Theresa Rohr, BA, HT(ASCP) Section Head, Histology Nyack Hospital 160 North Midland Avenue Nyack, New York 10960 phone 845-348-2276 fax 845-348-8430 Nyack Hospital is required by Law to protect the privacy of health information under HIPAA Rules and Regulations Part 11, Article 45 CFR, Parts 160-164. This email, including attachments, is intended for the exclusive use of the person or the entity to which it is addressed. It may contain confidential or legally protected information. The authorized recipient is prohibited from disclosing this information to any other party and is required to destroy this information after its stated need has been fulfilled. If the recipient or reader of this email is not the intended recipient or her/his authorized agent, the reader is hereby notified that any dissemination, distribution or copying of this email is prohibited. If you believe that you have received this email in error, please advise the sender immediately by reply email and then delete this email immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. PC-to-Phone calls for ridiculously low rates. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From tmhpath <@t> amigo.net Wed Mar 29 16:07:58 2006 From: tmhpath <@t> amigo.net (Michelle D. Moore) Date: Wed Mar 29 16:10:18 2006 Subject: [Histonet] Med Tech forum like Histonet? Message-ID: <001d01c6537d$3d067a90$ef00a8c0@tmhpath1> Hello hope everyone is having a good day :) I have a friend that is a Med Tech and she was wondering if there was a forum like the Histonet only for Med Techs. She is in need of another resource for some information. If you are a Med Tech and can help please let me know or if you would be willing to consult with her on some issues that would be nice also. Thank you for you time and help. Have a Grateful Day :) Michelle D. Moore HT(ASCP) TMH 785 Russell St Craig, CO 81625 tmhpath@amigo.net From dpconsult <@t> earthlink.net Wed Mar 29 17:18:01 2006 From: dpconsult <@t> earthlink.net (Dick Paulson [Source Medical Products]) Date: Wed Mar 29 17:18:11 2006 Subject: [Histonet] Microwave Tissue hardening Message-ID: Jim, The best place to start would be to contact Donna Willis at Milestone Medical. Donna is the National Applications Specialist. Phone : (972) 606-9986 EMail : donna@milestonemed.com Source Medical Products Dick Paulson ---- Original Message ---- Greeting all, My next question is on tissue hardening. We are using the Milestone RHS-1 microwave processor. There is a program for tissue hardening but no time frame. Any suggestions on how long to what volume of tissue to use? And how about which to use, either 10% formalin at 50*C or PBS at 65*C? I am just starting this project so please bare with me as I will probably ask many questions as I go along. Thank you for all your assistance. Jim Bradford From amosbrooks <@t> gmail.com Wed Mar 29 17:56:26 2006 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Wed Mar 29 17:56:29 2006 Subject: [Histonet] Re: Her2 Fish Testing with Sakura Rapid Tissue Message-ID: <582736990603291556q6c6b68b9vfdd96a5cbe7b1177@mail.gmail.com> Hi, If anyone has an answer to Leslie's question could you please post it to the list, or CC me as well as I am interested in this as well. Our lab is going to be getting one of these shortly as well. Thanks, Amos Brooks Date: Wed, 29 Mar 2006 09:38:45 -0500 > From: lksell@aol.com > Subject: [Histonet] Her2 Fish Testing with Sakura Rapid Tissue > Processor > To: Histonet@lists.utsouthwestern.edu > Message-ID: <8C821559E71DC62-5FC-7329@MBLK-R05.sysops.aol.com> > Content-Type: text/plain; charset="us-ascii" > > Is there anyone out there who is doing Her2 Fish testing with breast core > biopsies that have been processed on the Rapid Tissue Processor from Sakura. > > Leslie Sell > Technical Coordinator > Presbyterian Hospital > Charlotte, NC From Kim.Osullivan <@t> med.monash.edu.au Wed Mar 29 18:46:33 2006 From: Kim.Osullivan <@t> med.monash.edu.au (Kim O'Sullivan) Date: Wed Mar 29 18:47:07 2006 Subject: [Histonet] Frozen embedding of lungs Message-ID: <130.194.113.116.1143679180@my.monash.edu.au> Hi all, Does any one out there have a successful method for inflating mouse lungs to freeze. We currently freeze them unfixed in OCT, but of course the morphology is shocking as the lungs have collapsed. Is there a method that preserves the morphololgy without collapsing or imploding the lungs. Any advice will be appreciated Kim O'Sullivan Centre for Inflammatory Diseases Department of Medicine Monash University Melbourne Australia From miller <@t> coho.net Wed Mar 29 18:49:23 2006 From: miller <@t> coho.net (Diane G. Miller) Date: Wed Mar 29 18:49:52 2006 Subject: [Histonet] Med Tech forum like Histonet? References: <001d01c6537d$3d067a90$ef00a8c0@tmhpath1> Message-ID: <091801c65393$ca0ac840$0200a8c0@desktop> There is a forum, here is the site: MEDLAB-L: http://www.ualberta.ca/~pletendr/MEDLAB-L.html Diane ----- Original Message ----- From: Michelle D. Moore To: Histonet Sent: Wednesday, March 29, 2006 2:07 PM Subject: [Histonet] Med Tech forum like Histonet? Hello hope everyone is having a good day :) I have a friend that is a Med Tech and she was wondering if there was a forum like the Histonet only for Med Techs. She is in need of another resource for some information. If you are a Med Tech and can help please let me know or if you would be willing to consult with her on some issues that would be nice also. Thank you for you time and help. Have a Grateful Day :) Michelle D. Moore HT(ASCP) TMH 785 Russell St Craig, CO 81625 tmhpath@amigo.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jkiernan <@t> uwo.ca Wed Mar 29 22:47:41 2006 From: jkiernan <@t> uwo.ca (John Kiernan) Date: Wed Mar 29 22:46:48 2006 Subject: [Histonet] Frozen embedding of lungs In-Reply-To: <130.194.113.116.1143679180@my.monash.edu.au> References: <130.194.113.116.1143679180@my.monash.edu.au> Message-ID: <442B62ED.10302@uwo.ca> Why not inflate with air (Pasteur pipette in trachea) and then plunge into isopentane (cooled by liquid nitrogen) or acetone (with dry ice ion it). Neither is ideal, but both freezing methods protect quite well against ice crystal damage; the crystal holes are much smaller than cells. Coating a specimen with goop before freezing ensures that the cells of the specimen are the last and most slowly frozen things in the blob. That makes for maximum damage by ice crystal formation. With slow freezing most of the the ice crystal holes are about the same size as a cell, and the tissue architecture is 100% wrecked. Unfixed objects must be frozen alost instantly. A blob of hydrophilic polymer can be added afterwards to protect against freeze-drying of stored specimen. OCT (whatever it is; ?polyvinyl alcohol) is a physical support for specimens being sectioned with a cryostat or an olde-worlde freezing microtome. It does not infiltrate the tissue in the way paraffin wax enters every dehydrated crevice. Most of the functions of OCT are duplicated by other hydrohilic polymers such as gelatin or agar. The abbreviation OCT is for Optimum Cutting Temperature. That has nothing to do with protection against freezing artifacts. It relates to the hardness of the polymer at some temperature that was, many years ago, considered optimal for cutting frozen sections. John Kiernan London, Canada ----------------------- Kim O'Sullivan wrote: > > Hi all, > > Does any one out there have a successful method for inflating mouse lungs to freeze. We currently freeze them unfixed in OCT, but of course the morphology is shocking as the lungs have collapsed. Is there a method that preserves the morphololgy without collapsing or imploding the lungs. > > Any advice will be appreciated > > Kim O'Sullivan > Centre for Inflammatory Diseases > Department of Medicine > Monash University > Melbourne > Australia > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo.rogerson <@t> waht.swest.nhs.uk Thu Mar 30 01:20:36 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Mar 30 01:19:54 2006 Subject: [Histonet] Frozen embedding of lungs Message-ID: When I was a pup I made a little 'lung tank'; you construct a piece of apparatus with a header tank at height to produce 1 atmosphere of pressure (can't remember the calculation but I think it was a metre), fill header tank with 10% buffered formalin and attached to trachea with a pipe. The pressure will inflate the lung but formalin will drip out; I put a reservoir beneath and then pumped the formalin back up to the header. I never used a respirator or anything and I guess that's why people say I look young for me age; use a respirator!!! I used it a lot for miner's black lung in an area that had coal mining for pneocony.... pneumoconiu..... um... Miner's Black lung! Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 "Following the line of least resistance makes both men and rivers crooked" This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Kim O'Sullivan [mailto:Kim.Osullivan@med.monash.edu.au] Sent: Thursday, March 30, 2006 1:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen embedding of lungs Hi all, Does any one out there have a successful method for inflating mouse lungs to freeze. We currently freeze them unfixed in OCT, but of course the morphology is shocking as the lungs have collapsed. Is there a method that preserves the morphololgy without collapsing or imploding the lungs. Any advice will be appreciated Kim O'Sullivan Centre for Inflammatory Diseases Department of Medicine Monash University Melbourne Australia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gillian.2.brown <@t> gsk.com Thu Mar 30 01:56:37 2006 From: gillian.2.brown <@t> gsk.com (gillian.2.brown@gsk.com) Date: Thu Mar 30 01:56:56 2006 Subject: [Histonet] Frozen embedding of lungs In-Reply-To: <130.194.113.116.1143679180@my.monash.edu.au> Message-ID: Hi Kim, this is what we are doing for Laser Capture Microdissection. If you are only doing IHC you could leave out the DEPC or even add in some protease inhibitors. We find it is better to replace the air with something. I have actually laid the lungs out as the pair onto their dorsal surface rather than cutting into pieces, it takes a little longer to freeze but we have no ice damage except in the muscle surrounding the oesophagus which has remained attached. The thoracic cavity is opened carefully to avoid puncturing the lungs. The trachea is exposed by a midline neck incision and an Angiocathw catheter (22GA, 1IN) (Becton Dickinson Co., Franklin Lakes, NJ) is inserted in the trachea and tied with 4-O silk (Ethicon Inc., Somerville, NJ) to secure the catheter. By gentle pressure applied to a 1.0 cm3 syringe attached to the catheter the lungs are inflated with Tissue-Tek OCT (Sakura Finetek USA, Torrance, CA) (50% v/v) in RNase (DPEC [diethylpyrocarbonate])- free phosphate-buffered saline with 10% sucrose. The total volume instilled is, 0.6 ml. The lungs are removed from the thoracic cavity gently and then cut sagitally into several pieces ,10 mm x 10 mm, placed in the base of cryomolds (Sakura ), covered with additional Tissue-Tek OCT, and frozen immediately in liquid nitrogen-cold 2-methylbutane (usually ,10 s). The pieces of lung are kept flat in the bottom of the cryomold to maximize the area of tissue that can be sectioned. The frozen tissue blocks are stored individually, wrapped in aluminum foil, at -80oC until sectioning begins. The tissue is sectioned at 7 micron using a cryostat and a minimum of 20 sections per animal is prepared for LCM. The frozen sections are placed on plain glass slides (no adhesive) and then immediately fixed in 100% ethanol for 1 min, followed by rehydration through 95, 70, and 50% ethanol diluted in DPEC-deionized H2O (5 s each). The sections are then stained with 0.5% (w/v) Nissl (cresyl violet acetate) (Sigma Chemical, St Louis, MO)/0.1 M sodium acetate-DPEC buffer for 1 min; dehydrate in graded ethanols (5 s each); and clean in xylene for 5 min. The slides are allowed to air-dry completely and are then stored desiccated to prevent activation of endogenous RNase in the tissues. Gill Brown Target Localisation Target Discovery GlaxoSmithKline Medicines Research Centre, "Kim O'Sullivan" Sent by: histonet-bounces@lists.utsouthwestern.edu 30-Mar-2006 01:46 To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Frozen embedding of lungs Hi all, Does any one out there have a successful method for inflating mouse lungs to freeze. We currently freeze them unfixed in OCT, but of course the morphology is shocking as the lungs have collapsed. Is there a method that preserves the morphololgy without collapsing or imploding the lungs. Any advice will be appreciated Kim O'Sullivan Centre for Inflammatory Diseases Department of Medicine Monash University Melbourne Australia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louise.renton <@t> gmail.com Thu Mar 30 02:00:43 2006 From: louise.renton <@t> gmail.com (louise renton) Date: Thu Mar 30 02:00:54 2006 Subject: [Histonet] colour change - slightly OT Message-ID: Hello all, in order to take things away from the (by now) heated discussions on unions and med techs, how about solving a little chemical mystery? My colleague is trying out a MMA home brew consisting of: MMA powder, di butyl pthalate (?sp) with the accelerator dimethyl analine. On placing these blocks in the incubator at 56deg C overnight, some of the blocks turned a deep mahogany brown. Those that were not exposed to air (ie kept in sealed bottles while polymerization took place) did not acchieve this dark hue, but remained a sort of translucent straw colour. SO- whats the chemistry here? any ideas - I am merely curious - the inetegrity pf the sections has not been compromised best regards -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "Does a conceited cowboy, only ride on pompous grass?. From louise.renton <@t> gmail.com Thu Mar 30 02:06:00 2006 From: louise.renton <@t> gmail.com (louise renton) Date: Thu Mar 30 02:06:08 2006 Subject: [Histonet] colour change - slightly OT Message-ID: Hello all, in order to take things away from the (by now) heated discussions on unions and med techs, how about solving a little chemical mystery? My colleague is trying out a MMA home brew consisting of: MMA powder, di butyl pthalate (?sp) with the accelerator dimethyl analine. On placing these blocks in the incubator at 56deg C overnight, some of the blocks turned a deep mahogany brown. Those that were not exposed to air (ie kept in sealed bottles while polymerization took place) did not acchieve this dark hue, but remained a sort of translucent straw colour. SO- whats the chemistry here? any ideas - I am merely curious - the inetegrity pf the sections has not been compromised best regards -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "Does a conceited cowboy, only ride on pompous grass?. From gmondragon <@t> gsopath.com Thu Mar 30 02:22:53 2006 From: gmondragon <@t> gsopath.com (Gus Mondragon) Date: Thu Mar 30 02:23:07 2006 Subject: [Histonet] P-75 NGFR5 Message-ID: <03B63DE44B5C014D84F9A9D8A968B5174C4D5A@lithium.corp.gsopath.com> This New Growth Factor monoclonal (P75) has been discontinued by vendor can someone tell me of an alternative antibody and vendor I can use. Thanks Gus Mondragon gmondragon@gsopath.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, March 27, 2006 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 28, Issue 44 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: colored OC T media - not TBS (Robyn Vazquez) 2. Mini auto stainers (Marian powers) ---------------------------------------------------------------------- Message: 1 Date: Mon, 27 Mar 2006 07:07:03 -0800 From: "Robyn Vazquez" Subject: Re: [Histonet] colored OC T media - not TBS To: mbmphoto@gmail.com, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Maria, We use the same OCT and add food coloring to it...works perfect. Make it in advanced and put it on a rotator after you shake it up. And you can experiment with different colors. Hope that helps... Robyn OHSU >>> "Maria Mejia" 3/23/2006 7:46 AM >>> Does anyone know where I can buy "colored" OCT freezing media that is NOT TBS media? Many thanks. Maria Bartola Mejia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Mon, 27 Mar 2006 12:46:12 -0500 From: "Marian powers" Subject: [Histonet] Mini auto stainers To: Message-ID: <000c01c651c6$56ab7710$be4dff45@MEGAN> Content-Type: text/plain; charset="iso-8859-1" Looking for a mini auto stainer other than Thermo's linistat. Thanks in advance. Marian ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 28, Issue 44 **************************************** From M.Prideaux <@t> sheffield.ac.uk Thu Mar 30 04:09:51 2006 From: M.Prideaux <@t> sheffield.ac.uk (Matt Prideaux) Date: Thu Mar 30 04:09:59 2006 Subject: [Histonet] Fw: Thermo/ Leica In-Reply-To: <001501c64e8a$785f4240$6501a8c0@acer311vpbceh0> References: <001501c64e8a$785f4240$6501a8c0@acer311vpbceh0> Message-ID: <1143713391.442bae6fa3529@webmail.shef.ac.uk> The leica rep that we deal with is called Anthony Johnson and he seems fairly helpful. His email address is anthony.johnson@leica-microsystems.com Matt -- Matt Prideaux Academic Unit of Bone Biology Division of Clinical Sciences (South) D Floor (DU20) Medical School Henry Wellcome Laboratories for Medical Research University of Sheffield Beech Hill Road Sheffield S10 2RX Tel: 0114 271 3783 Fax: 0114 271 1711 Quoting MattG : > Does anyone have a current email address for Pauline Fisher of Thermo > (formerly Shandon)? > > I'd also be grateful if someone could send me an electronic version of the > user manual for the Leica TP1050. > > If there are any UK based Leica or Thermo reps on here, could one of you > please drop me an email as I have a couple of questions I'd like to ask. > > Thanks, > > Matt Griffiths > > > > ___________________________________________________________ > Win a BlackBerry device from O2 with Yahoo!. Enter now. > http://www.yahoo.co.uk/blackberry > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ctsblack <@t> capeheart.uct.ac.za Thu Mar 30 05:45:52 2006 From: ctsblack <@t> capeheart.uct.ac.za (Melanie Black) Date: Thu Mar 30 05:52:13 2006 Subject: [Histonet] GMA & Humidity Message-ID: Dear All, I would like to ask about a particular problem with eosin-Y staining. I am using Eosin Y alcoholic without phloxine (from Sigma) and for some reason, all of the eosin staining comes out of the tissue as it is being dehydrated. I can actually see that it just drains out. I know that this is part of the differentiation process but it just all comes out. We use the same staining set for mouse liver tumour tissues with no problem. These are routinely processed (by a hospital pathology lab) rat rectum slides. Is the phloxine important for "keeping in the stain"? The sections are quite small. Should I be using a different eosin? Any advice on this matter would be greatly appreciated. Cathy Cathy JB 4 resin is one of the few resins that is water soluble, and complete dehydration of tissue is NOT required. In the processing of this resin, clearing agents like xylene & chloroform are unnecessary. I have used this resin many times, specially for tissue containing gels etc. Regards Melanie. -- Cardiovascular Research Unit Div. of Cardiothoracic Surgery Chris Barnard Building University of Cape Town Anzio Road Observatory 7925 Republic of South Africa Tel +27 21 406-6589 Cel +27 82 469-3352 Fax +27 21 448-5935 From pedro.louro <@t> spcorp.com Thu Mar 30 06:28:22 2006 From: pedro.louro <@t> spcorp.com (Louro, Pedro) Date: Thu Mar 30 06:28:53 2006 Subject: [Histonet] Frozen embedding of lungs Message-ID: Hi Kim, I have done this procedure before with good results. I used a 50:50 ratio of OCT and saline mixed well together into a syringe, then inflate the lungs with this mixture. I tried to use all OCT but it was impossible to inflate the lungs because the OCT was so thick. After inflation with the 50:50 ratio, I froze the lungs in OCT either of two ways: 1) Plastic embedding mold with OCT into Methyl Butane that is cooled with liquid nitrogen OR 2) Metal embedding mold with OCT into a slushy mix consisting of dry ice and Methyl Butane. Both give good results Hope this helps Pedro Louro Schering-Plough Research Institute -----Original Message----- From: Kim O'Sullivan [mailto:Kim.Osullivan@med.monash.edu.au] Sent: Wednesday, March 29, 2006 7:47 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen embedding of lungs Hi all, Does any one out there have a successful method for inflating mouse lungs to freeze. We currently freeze them unfixed in OCT, but of course the morphology is shocking as the lungs have collapsed. Is there a method that preserves the morphololgy without collapsing or imploding the lungs. Any advice will be appreciated Kim O'Sullivan Centre for Inflammatory Diseases Department of Medicine Monash University Melbourne Australia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From dobbin <@t> upei.ca Thu Mar 30 06:48:55 2006 From: dobbin <@t> upei.ca (Greg Dobbin) Date: Thu Mar 30 06:50:53 2006 Subject: [Histonet] Med Tech forum like Histonet? In-Reply-To: <001d01c6537d$3d067a90$ef00a8c0@tmhpath1> Message-ID: <442B9B77.9777.EE88A@acad1.cs.upei.ca> There is Medlab-L. (L for listserv). It is run out of the Univ of Alberta. Don't have the coordinates but Google will probably find it. Greg From: "Michelle D. Moore" To: "Histonet" Date sent: Wed, 29 Mar 2006 15:07:58 -0700 Organization: The Memorial Hospital Subject: [Histonet] Med Tech forum like Histonet? > Hello hope everyone is having a good day :) I have a friend that is a > Med Tech and she was wondering if there was a forum like the Histonet > only for Med Techs. She is in need of another resource for some > information. If you are a Med Tech and can help please let me know or > if you would be willing to consult with her on some issues that would > be nice also. Thank you for you time and help. Have a Grateful Day :) > > Michelle D. Moore HT(ASCP) > TMH > 785 Russell St > Craig, CO 81625 > tmhpath@amigo.net > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LGaliotto <@t> nch.org Thu Mar 30 07:40:27 2006 From: LGaliotto <@t> nch.org (Galiotto, Laura) Date: Thu Mar 30 07:40:34 2006 Subject: [Histonet] Breast core biopsies, same day results by frozen sections Message-ID: <270614B321ACB44D8C1D91F4F921FDC3036CBC74@NCH01EX02.nch.org> Is there anyone performing same day results on breast biopsies by frozen sections? If so please share the pros and cons. Has there been any false negative results? Laura Galiotto, HT (ASCP) Histology Facilitator Northwest Community Hospital 800 West Central Road Arlington Heights, Il 60005 847-618-6190 From GDawson <@t> dynacaremilwaukee.com Thu Mar 30 08:04:40 2006 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Thu Mar 30 08:04:30 2006 Subject: [Histonet] Glycophorin A Message-ID: Same Here. Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Dana Settembre Sent: Wednesday, March 29, 2006 1:03 PM To: histonet@lists.utsouthwestern.edu; Martha Ward Subject: Re: [Histonet] Glycophorin A Hello Martha, I use Glycophorin A from Dako cat# M0819 Dana Settembre UMDNJ - University Hospital Newark, NJ >>> Martha Ward 03/29/06 1:52 PM >>> One of our Pathologists have asked about working this antibody up to add to our menu. It is available from several vendors but I wanted to check and see which is most commonly used. We will be using it on the Ventana NexEs. Thanks in advance for your help with this. Martha Ward, MT ASCP (QIHC) Wake Forest University Baptist Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bill501 <@t> mindspring.com Thu Mar 30 08:14:33 2006 From: bill501 <@t> mindspring.com (Bill Blank) Date: Thu Mar 30 08:14:48 2006 Subject: [Histonet] Breast core biopsies, same day results by frozen sections In-Reply-To: <270614B321ACB44D8C1D91F4F921FDC3036CBC74@NCH01EX02.nch.org> References: <270614B321ACB44D8C1D91F4F921FDC3036CBC74@NCH01EX02.nch.org> Message-ID: Anyone using a FS on a breast biopsy to make a therapeutic decision is a barbarian and an idiot. Ditto if you waste core biopsy tissue on a FS. Bill At 7:40 AM -0600 3/30/06, Galiotto, Laura wrote: >Is there anyone performing same day results on breast biopsies by >frozen sections? If so please share the pros and cons. Has there >been any false negative results? -- I would be glad now to flee the shadow-shifting way back into the Otherwhere, to my own fair moon and softer sunlight. From Charles.Embrey <@t> carle.com Thu Mar 30 08:17:13 2006 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Thu Mar 30 08:17:31 2006 Subject: [Histonet] Breast core biopsies, same day results by frozen sections Message-ID: What a scary thought. The first real question is: What would be the benefit? Are you going to do the lumpectomy or mastectomy immediately following the frozen section result? Fat is difficult to cut on frozen and I shudder to think of all the wasted (and now non-diagnosable) tissue you will incur trying to get an adequate section. To me, a frozen section is only of value if it will directly impact a surgical decision that will result in immediate action. The biopsy is a unique specimen and even if you were to attempt to get more tissue you can't be certain that it will yield the same result. I can't see possibly wasting it on an unnecessary frozen section. Charles Embrey PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Galiotto, Laura Sent: Thursday, March 30, 2006 7:40 AM To: HISTONET@LISTS.UTSOUTHWESTERN.EDU Subject: [Histonet] Breast core biopsies, same day results by frozen sections Is there anyone performing same day results on breast biopsies by frozen sections? If so please share the pros and cons. Has there been any false negative results? Laura Galiotto, HT (ASCP) Histology Facilitator Northwest Community Hospital 800 West Central Road Arlington Heights, Il 60005 847-618-6190 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo.rogerson <@t> waht.swest.nhs.uk Thu Mar 30 08:32:20 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Mar 30 08:31:35 2006 Subject: [Histonet] Breast core biopsies, same day results by frozen sections Message-ID: Must agree totally with that. Rapid processing and then a conventional H&E can be done in less than 3 hours; the same day. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 "Following the line of least resistance makes both men and rivers crooked" This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Bill Blank [mailto:bill501@mindspring.com] Sent: Thursday, March 30, 2006 3:15 PM To: HISTONET@LISTS.UTSOUTHWESTERN.EDU Subject: Re: [Histonet] Breast core biopsies, same day results by frozen sections Anyone using a FS on a breast biopsy to make a therapeutic decision is a barbarian and an idiot. Ditto if you waste core biopsy tissue on a FS. Bill At 7:40 AM -0600 3/30/06, Galiotto, Laura wrote: >Is there anyone performing same day results on breast biopsies by >frozen sections? If so please share the pros and cons. Has there >been any false negative results? -- I would be glad now to flee the shadow-shifting way back into the Otherwhere, to my own fair moon and softer sunlight. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cormier <@t> MIT.EDU Thu Mar 30 08:57:29 2006 From: cormier <@t> MIT.EDU (Kathy Cormier) Date: Thu Mar 30 08:57:16 2006 Subject: [Histonet] brady labexpert labeler Message-ID: <003901c6540a$442995e0$92003712@mit.edu> Greeting Histonet, Has anyone in research tried out this device or anything similar? We are thinking about getting this device to label all our hundreds of serology and PCR tubes... Thanks! Kathy DCM MIT From leswes <@t> shaw.ca Thu Mar 30 09:12:49 2006 From: leswes <@t> shaw.ca (Lesley Weston) Date: Thu Mar 30 09:11:28 2006 Subject: [Histonet] Health and Safety Guidelines for the Lab by L Montgomery needed In-Reply-To: Message-ID: You could try Abebooks at http://www.abebooks.com/ They seem to be able to find everything. Lesley Weston > From: jason.burrill@crl.com > Date: Wed, 29 Mar 2006 13:25:00 -0500 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Health and Safety Guidelines for the Lab by L Montgomery > needed > > Hello all, > I am in the process of taking the SLS (Specialist in Laboratory Safety) > certification from ASCP and can not locate a new or used text of "Health > and Safety Guidelines for the Laboratory" by Lynn Montgomery. I have > searched the internet, Amazon and contacted ASCP with no luck (out of > print), if anyone has any suggestions on where else I can look I would > really appreciate it. > Thanks in advance for your help. > Jason > > > Jason D. Burrill > Manager, Histology > Charles River Laboratories > 251 Ballardvale Street > Wilmington, MA 01887 > ph: 978-658-6000 ext 1652 > fax: 978-988-8793 > E-mail: jason.burrill@crl.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> u.arizona.edu Thu Mar 30 09:18:16 2006 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Thu Mar 30 09:18:28 2006 Subject: [Histonet] insect histology book inquiry Message-ID: <4.3.2.7.2.20060330081133.00cdc960@algranth.inbox.email.arizona.edu> Good Thursday morning! Is there anybody out there in histoland who might have a copy of the now out of print book, A Manual of Basic Techniques in Insect Histology by P. Barbosa, Autumn Publishers, 1974? I'm looking for details on Stile's butyl alcohol technique that was discussed in a paper on whiteflies and Barbosa was cited. A jillion thanks to anybody who can provide this technique! Andi Grantham ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From gcallis <@t> montana.edu Thu Mar 30 09:24:18 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Mar 30 09:24:25 2006 Subject: Detailed reply on how to Re: [Histonet] Frozen embedding of lungs In-Reply-To: <130.194.113.116.1143679180@my.monash.edu.au> References: <130.194.113.116.1143679180@my.monash.edu.au> Message-ID: <6.0.0.22.1.20060330074745.01b6b008@gemini.msu.montana.edu> We fill the lungs with OCT (other cryoembedding medias may not work well if they have only been tested on human tissues). We discovered this the hard way, so test your cryomedia for murine work. Equipment needed 3 ml syringe with dulled 18 gauge needle. You do NOT want to have the sharp cutting edges left on the bevel. Fill syringe with OCT and make sure there are no bubbles in OCT. Tissue Tek plastic embedding molds, dry ice/isopentane slurry for snap freezing. NO CRYOSTAT freezing. If you want another snap freezing method that eliminates the solvent, let me know, I will attach privately. Cuticle scissors (stainless steel) found at drugstores, they are curved, cheap and have the finest tips for what you are going to do. These are excellent dissection tools for tiny murine tissues. Some type of blunt probe. Mosquito hemostats with narrow, fine ends. Euthanize the mouse with anesthetic overdose or CO2 gas since cervical dislocation disrupts tissues and trachea. Open abdomen up to lower jaw to expose lungs with heart attached by cutting through the ribs and splaying these out so the lung and heart are visible along with the trachea. Severe liver attachments from the lung cavity, and bleed out the animal by severing ascending aorta and veins behind the intestines, and use a PBS damp gauze sponge to soak up excess blood. Make sure you DO NOT CUT THE TRACHEA nor disrupt it but gently expose it by teasing away muscle and fascia around trachea. A blunt probe works very well for this purpose. To minimize fur flying around, wet the animal with PBS, not alcohol (that is a fixative) before opening the abdomen, pin down legs, nose with hypodermic needles so body does not move around. About half way down the trachea from the jaw area (better to be closer to jaw than ribs), make a v shaped cut on the very top of the trachea but DO NOT CUT across the trachea or the trachea retracts into lung cavity. Use the fine tipped scissors to make the v shaped cut. Insert 18 gauge needle into v shaped cut on top of the trachea. Keep the needle flat and do not shove it in with force, you do not want to puncture the trachea, and slide it so you can see the dulled bevel facing up but into the lumen of the trachea. Inject OCT into the lung and watch it fill but use only 2 to 2 1/2 mls or you will blow away the alveoli. You want the lungs to be filled in the most lifelike form and size - not a tissue balloon. Once you have filled the lung, take the mosquito hemostat, clamp off the trachea below the needle bevel so you can gently lift and cut the trachea. Gently dissect the lung with heart from pleural cavity - it will come out intact using the hemostat to prevent OCT leakage from the lung (backwash!). Using curved scissors, remove heart from the lung (unless you want to see heart) embed the lung whole OR cut off the lobe you need, and snap freeze immediately. Blocks can be stored at -80C for future dates ( years later!) We cryosection our OCT filled lungs at -20C, and never have problems with morphology nor exploded alveoli. The latter is because care is take to NOT overfill the lungs with OCT. The OCT does NOT have to be diluted, if it is, it tends to leak or diffuse out from tissue. We have even used Thermo Electrons green cryoembedding media which creates an interesting contrast of lung tissue to the green goo but does not affect the sectioning qualities! If you have any questions, contact me. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From Barry.R.Rittman <@t> uth.tmc.edu Thu Mar 30 09:27:16 2006 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Thu Mar 30 09:27:20 2006 Subject: [Histonet] insect histology book inquiry Message-ID: Andi I think that the N-butyl alcohol method is OK and some original methods used this because it is miscible with paraffin wax. However if an intermediary agent is not used then although the tissue remains softer than when using ethanol, the butyl alcohol takes much longer to be replaced by the paraffin wax. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea Grantham Sent: Thursday, March 30, 2006 9:18 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] insect histology book inquiry Good Thursday morning! Is there anybody out there in histoland who might have a copy of the now out of print book, A Manual of Basic Techniques in Insect Histology by P. Barbosa, Autumn Publishers, 1974? I'm looking for details on Stile's butyl alcohol technique that was discussed in a paper on whiteflies and Barbosa was cited. A jillion thanks to anybody who can provide this technique! Andi Grantham ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From froyer <@t> bitstream.net Thu Mar 30 09:45:11 2006 From: froyer <@t> bitstream.net (Ford Royer) Date: Thu Mar 30 09:45:27 2006 Subject: [Histonet] Breast core biopsies, same day results by frozen sections In-Reply-To: Message-ID: <002e01c65410$ee8e4930$6f01a80a@fords> WOW... have times changed(?). In my day (1970s), the SOP was to do STAT frozen sections on breast biopsies right in the surgery suite while the patient was still under anesthesia. The rationale was that if the biopsy was positive, they could continue with the mastectomy while the pt. was still under. The feeling (that I was told at the time) was that it was too dangerous to put the patient under general anesthesia twice; once for the biopsy, and then again a few days later for the mastectomy. Of course back then, they felt that if you extracted all the patient's teeth that it would cure their arthritis, and of course frontal lobotomies where state of the art for those with schizophrenia. ~ Ford Ford M. Royer, MT(ASCP) Minnesota Medical Specialists, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 763-542-8725 phone 888-790-9686 toll free 763-546-4830 fax email web -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles.Embrey Sent: Thursday, March 30, 2006 8:17 AM To: Galiotto, Laura; HISTONET@LISTS.UTSOUTHWESTERN.EDU Subject: RE: [Histonet] Breast core biopsies,same day results by frozen sections What a scary thought. The first real question is: What would be the benefit? Are you going to do the lumpectomy or mastectomy immediately following the frozen section result? Fat is difficult to cut on frozen and I shudder to think of all the wasted (and now non-diagnosable) tissue you will incur trying to get an adequate section. To me, a frozen section is only of value if it will directly impact a surgical decision that will result in immediate action. The biopsy is a unique specimen and even if you were to attempt to get more tissue you can't be certain that it will yield the same result. I can't see possibly wasting it on an unnecessary frozen section. Charles Embrey PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Galiotto, Laura Sent: Thursday, March 30, 2006 7:40 AM To: HISTONET@LISTS.UTSOUTHWESTERN.EDU Subject: [Histonet] Breast core biopsies, same day results by frozen sections Is there anyone performing same day results on breast biopsies by frozen sections? If so please share the pros and cons. Has there been any false negative results? Laura Galiotto, HT (ASCP) Histology Facilitator Northwest Community Hospital 800 West Central Road Arlington Heights, Il 60005 847-618-6190 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Thu Mar 30 09:48:44 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Thu Mar 30 09:50:39 2006 Subject: [Histonet] Rapid Decal References: Message-ID: We get our Rapid Decal Immuno from BCC company (1-800-635-4477). We have 14 Pathologists and they are extremely pleased with the results. Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Richard Cartun Sent: Wed 3/29/2006 3:56 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Rapid Decal Is anyone using "Rapid Decal" for bone marrow core biopsies? If so, how do you like it (advantages vs. disadvantages)? Where do you get it? Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From oshel1pe <@t> cmich.edu Thu Mar 30 10:01:40 2006 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Thu Mar 30 10:01:51 2006 Subject: [Histonet] insect histology book inquiry In-Reply-To: <4.3.2.7.2.20060330081133.00cdc960@algranth.inbox.email.arizona.edu> References: <4.3.2.7.2.20060330081133.00cdc960@algranth.inbox.email.arizona.edu> Message-ID: Andrea, "Stiles' n-butyl alcohol technique Recommended as particularly useful for small insects. Formula: Solution A ethyl alcohol (45%) 90 pt n-butyl alcohol 10 pt Solution B ethyl alcohol (62%) 80 pt n-butyl alcohol 20 pt Solution C ethyl alcohol (77%) 65 pt n-butyl alcohol 35 pt Solution D ethyl alcohol (90%) 45pt n-butyl alcohol 55 pt Solution E ethyl alcohol (abs) 2 pt n-butyl alcohol 1 pt Solution F Paraffin (m.p. 56-58 deg C) 2 pt n-butyl alcohol 1 pt Procedure: 1. After fixation (e.g. Gilson's) place in ethyl alcohol (35%) for 30 min. to 1 hr. 2. Transfer to solution A for 2 hr. 3. Transfer to solution B for 2 hr. 4. Transfer to solution C for 4 hr. 5. Transfer to solution D for 6 hr. to several days. 6. Transfer to solution E for 6 hr. to overnight (with one change) 7. Transfer through 2 changes of n-butyl alcohol (at several hours interval). 8. Transfer to solution F in covered dish (in oven) for 12 to 24 hr. 9. Uncover, dish until n-butyl odor dissipates. 10. Transfer to paraffin (optional). 11. A long infiltration period (4-5 days) is recommended for best results (causes less hardening). 12. Embed in paraffin wax (56-58 deg C m.p.) with slight addition of bayberry wax (<=3%). Note: 1. The choice of fixative is important so that no hardening factors are added to the procedure. 2. Specimens may be left in n-butyl (step 7) for a very long period of time." ref. cited: Stiles, K.A. 1934. Normal butyl alcohol technic for animal tissues with special reference to insects. Stain Technol. 9:97-100. In: Barbosa, P. 1974. Manual of Basic Techniques in Insect Histology. Autumn Publishers, Amherst, MA. Sounds like a relaxing technique. Phil >Good Thursday morning! >Is there anybody out there in histoland who might have a copy of the >now out of print book, A Manual of Basic Techniques in Insect >Histology by P. Barbosa, Autumn Publishers, 1974? >I'm looking for details on Stile's butyl alcohol technique that was >discussed in a paper on whiteflies and Barbosa was cited. >A jillion thanks to anybody who can provide this technique! >Andi Grantham >..................................................................... >: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : >: Sr. Research Specialist University of Arizona : >: (office: AHSC 4212) P.O. Box 245044 : >: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : >: (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : >:...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Philip Oshel Microscopy Facility Supervisor Department of Biology Central Michigan University 024C Brooks Hall Mt. Pleasant, MI 48859 (989) 774-3576 From lpjones <@t> srhs-pa.org Thu Mar 30 11:04:30 2006 From: lpjones <@t> srhs-pa.org (Jones, Laura) Date: Thu Mar 30 11:04:35 2006 Subject: [Histonet] CAP Immuno Survey Slides Message-ID: <8E7AD740937B954F947F0DB4467EFEE056E973@mail.srhs-pa.org> Hello Histoland. We were wondering if anyone else who may have participated in the recent CAP survey had experienced any problems with the labels on the slides. We performed some of the survey by hand (our machine is down!) using the Shandon Sequenza coverplates, and it seemed like the label adhesive was blocking the reagents from passing onto the slide. We then repeated some of the slides on a demo machine, after verifying our antibodies, and even then it seemed like that adhesive was interfering with the reagents. We also lost any info we had written on the slide labels, even with the impervious markers, during automated processing. We didn't remember our survey slides ever having labels before, and are just curious if anyone else had encountered this. Thanks in advance! The Histochicks at Sharon Regional, Sharon, PA From lrichey <@t> u.washington.edu Thu Mar 30 11:29:07 2006 From: lrichey <@t> u.washington.edu (Lori Richey) Date: Thu Mar 30 11:29:30 2006 Subject: [Histonet] CD10 In-Reply-To: References: Message-ID: <442C1563.3030209@u.washington.edu> We use Novocastro CD10 at 1:50 diluted in Monet Blue. Pre treatment is 15 min microwave in EDTA pH 8, biotinylated secondary, with Elite (Vecta Stain ABC kit) Theresa Rohr wrote: >Hello, > >I was wondering if anyone has any suggestions regarding CD10. I have the Dako Autostainer and use the Envision+ system and a Steamer for Antigen Retrieval. > >Since Dako only has an antibody for use on Frozen sections, I am searching for one for FFPE tissue. My Dako Rep actually suggested Novocastro as having a great antibody. > >Does anyone out there agree/disagree??? >At what dilution???? > >What I already researched looks like this is not a cheap antibody in terms of dillution. > >I would appreciate any help. > >Thanks so much, >Theresa Rohr, Nyack Hospital >rohrt@nyackhospital.org > >Theresa Rohr, BA, HT(ASCP) >Section Head, Histology >Nyack Hospital >160 North Midland Avenue >Nyack, New York 10960 >phone 845-348-2276 >fax 845-348-8430 > >Nyack Hospital is required by Law to protect the privacy of health information under HIPAA Rules and Regulations Part 11, Article 45 CFR, Parts 160-164. This email, including attachments, is intended for the exclusive use of the person or the entity to which it is addressed. It may contain confidential or legally protected information. The authorized recipient is prohibited from disclosing this information to any other party and is required to destroy this information after its stated need has been fulfilled. If the recipient or reader of this email is not the intended recipient or her/his authorized agent, the reader is hereby notified that any dissemination, distribution or copying of this email is prohibited. If you believe that you have received this email in error, please advise the sender immediately by reply email and then delete this email immediately. > ! > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From juan.gutierrez <@t> christushealth.org Thu Mar 30 11:44:47 2006 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Thu Mar 30 11:47:00 2006 Subject: [Histonet] CAP Immuno Survey Slides Message-ID: We pulled the labels off. What a pain in the .... To: CAP, please don't do that again. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jones, Laura Sent: Thursday, March 30, 2006 11:05 AM To: Histonet (E-mail) Subject: [Histonet] CAP Immuno Survey Slides Hello Histoland. We were wondering if anyone else who may have participated in the recent CAP survey had experienced any problems with the labels on the slides. We performed some of the survey by hand (our machine is down!) using the Shandon Sequenza coverplates, and it seemed like the label adhesive was blocking the reagents from passing onto the slide. We then repeated some of the slides on a demo machine, after verifying our antibodies, and even then it seemed like that adhesive was interfering with the reagents. We also lost any info we had written on the slide labels, even with the impervious markers, during automated processing. We didn't remember our survey slides ever having labels before, and are just curious if anyone else had encountered this. Thanks in advance! The Histochicks at Sharon Regional, Sharon, PA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Thu Mar 30 11:59:26 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Mar 30 12:10:20 2006 Subject: [Histonet] CAP Immuno Survey Slides Message-ID: I agree. I will forward this to a member of the CAP Cell Markers Committee. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "GUTIERREZ, JUAN" 03/30/06 12:44PM >>> We pulled the labels off. What a pain in the .... To: CAP, please don't do that again. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jones, Laura Sent: Thursday, March 30, 2006 11:05 AM To: Histonet (E-mail) Subject: [Histonet] CAP Immuno Survey Slides Hello Histoland. We were wondering if anyone else who may have participated in the recent CAP survey had experienced any problems with the labels on the slides. We performed some of the survey by hand (our machine is down!) using the Shandon Sequenza coverplates, and it seemed like the label adhesive was blocking the reagents from passing onto the slide. We then repeated some of the slides on a demo machine, after verifying our antibodies, and even then it seemed like that adhesive was interfering with the reagents. We also lost any info we had written on the slide labels, even with the impervious markers, during automated processing. We didn't remember our survey slides ever having labels before, and are just curious if anyone else had encountered this. Thanks in advance! The Histochicks at Sharon Regional, Sharon, PA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From settembr <@t> umdnj.edu Thu Mar 30 12:39:30 2006 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Thu Mar 30 12:40:32 2006 Subject: [Histonet] CAP Immuno Survey Slides Message-ID: Please do not send us slides with labels. They were really difficult to work with. Dana Settembre University Hospital - UMDNJ Newark, NJ >>> Richard Cartun 03/30/06 12:59 PM >>> I agree. I will forward this to a member of the CAP Cell Markers Committee. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "GUTIERREZ, JUAN" 03/30/06 12:44PM >>> We pulled the labels off. What a pain in the .... To: CAP, please don't do that again. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jones, Laura Sent: Thursday, March 30, 2006 11:05 AM To: Histonet (E-mail) Subject: [Histonet] CAP Immuno Survey Slides Hello Histoland. We were wondering if anyone else who may have participated in the recent CAP survey had experienced any problems with the labels on the slides. We performed some of the survey by hand (our machine is down!) using the Shandon Sequenza coverplates, and it seemed like the label adhesive was blocking the reagents from passing onto the slide. We then repeated some of the slides on a demo machine, after verifying our antibodies, and even then it seemed like that adhesive was interfering with the reagents. We also lost any info we had written on the slide labels, even with the impervious markers, during automated processing. We didn't remember our survey slides ever having labels before, and are just curious if anyone else had encountered this. Thanks in advance! The Histochicks at Sharon Regional, Sharon, PA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From livieira <@t> ualg.pt Thu Mar 30 13:11:06 2006 From: livieira <@t> ualg.pt (Lina Vieira) Date: Thu Mar 30 13:12:47 2006 Subject: [Histonet] Aluminium localization Message-ID: <007301c6542d$b28574f0$2914100a@labhistologia> In return to the Aluminium localization, I would now if an excessive time of fixation (in formaldeide), or an excessive time of etanol 70% imersion (for example 4 days)can induce false negative results to the aluminum? Thanks in advance for any information you can provide, Lina Vieira University of Algarve Portugal _______________________________________________ From GDawson <@t> dynacaremilwaukee.com Thu Mar 30 13:14:47 2006 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Thu Mar 30 13:14:41 2006 Subject: [Histonet] CAP Immuno Survey Slides Message-ID: All, I've talked to the CAP about this issue and they said that they would "take it under advisement". Just goes to show you that those in charge of sending out these CAP surveys for IHC have no experience in the actual staining of IHC slides. Also, the labels they put on are heavy duty and the adhesive they leave behind is NOT condusive to automated coverslipping (with the Sakura Glass coverslipper). They tend to fall off the suction cup and get broken by the conveyor. I would strongly suggest coverslipping these slides by hand until the CAP decides to stop this craziness. Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Dana Settembre Sent: Thursday, March 30, 2006 12:40 PM To: JUAN GUTIERREZ; Richard Cartun; Histonet (E-mail); Laura Jones Subject: RE: [Histonet] CAP Immuno Survey Slides Please do not send us slides with labels. They were really difficult to work with. Dana Settembre University Hospital - UMDNJ Newark, NJ >>> Richard Cartun 03/30/06 12:59 PM >>> I agree. I will forward this to a member of the CAP Cell Markers Committee. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "GUTIERREZ, JUAN" 03/30/06 12:44PM >>> We pulled the labels off. What a pain in the .... To: CAP, please don't do that again. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jones, Laura Sent: Thursday, March 30, 2006 11:05 AM To: Histonet (E-mail) Subject: [Histonet] CAP Immuno Survey Slides Hello Histoland. We were wondering if anyone else who may have participated in the recent CAP survey had experienced any problems with the labels on the slides. We performed some of the survey by hand (our machine is down!) using the Shandon Sequenza coverplates, and it seemed like the label adhesive was blocking the reagents from passing onto the slide. We then repeated some of the slides on a demo machine, after verifying our antibodies, and even then it seemed like that adhesive was interfering with the reagents. We also lost any info we had written on the slide labels, even with the impervious markers, during automated processing. We didn't remember our survey slides ever having labels before, and are just curious if anyone else had encountered this. Thanks in advance! The Histochicks at Sharon Regional, Sharon, PA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jason.burrill <@t> crl.com Thu Mar 30 13:22:19 2006 From: jason.burrill <@t> crl.com (jason.burrill@crl.com) Date: Thu Mar 30 13:22:44 2006 Subject: [Histonet] Re: Health and Safety Guidelines for the Lab by L Montgomery needed In-Reply-To: <19E3602A16438E48B51A4250CA04B5F6591564@exchange.marketlab.com> Message-ID: Thanks to all that responded and all your offers. Thanks to Victor Tobias I found a used copy at http://www.abebooks.com. It is a very difficult book to get your hands on. Jason Jason D. Burrill Manager, Histology Charles River Laboratories 251 Ballardvale Street Wilmington, MA 01887 ph: 978-658-6000 ext 1652 fax: 978-988-8793 E-mail: jason.burrill@crl.com From JMacDonald <@t> mtsac.edu Thu Mar 30 13:25:29 2006 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Mar 30 13:25:46 2006 Subject: [Histonet] eosin Y In-Reply-To: Message-ID: What is the pH of the eosin? Melanie Black Sent by: histonet-bounces@lists.utsouthwestern.edu 03/30/2006 03:45 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] GMA & Humidity Dear All, I would like to ask about a particular problem with eosin-Y staining. I am using Eosin Y alcoholic without phloxine (from Sigma) and for some reason, all of the eosin staining comes out of the tissue as it is being dehydrated. I can actually see that it just drains out. I know that this is part of the differentiation process but it just all comes out. We use the same staining set for mouse liver tumour tissues with no problem. These are routinely processed (by a hospital pathology lab) rat rectum slides. Is the phloxine important for "keeping in the stain"? The sections are quite small. Should I be using a different eosin? Any advice on this matter would be greatly appreciated. Cathy Cathy JB 4 resin is one of the few resins that is water soluble, and complete dehydration of tissue is NOT required. In the processing of this resin, clearing agents like xylene & chloroform are unnecessary. I have used this resin many times, specially for tissue containing gels etc. Regards Melanie. -- Cardiovascular Research Unit Div. of Cardiothoracic Surgery Chris Barnard Building University of Cape Town Anzio Road Observatory 7925 Republic of South Africa Tel +27 21 406-6589 Cel +27 82 469-3352 Fax +27 21 448-5935 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Thu Mar 30 13:45:14 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Mar 30 13:45:20 2006 Subject: [Histonet] Aluminium localization Message-ID: <09C945920A6B654199F7A58A1D7D1FDE017176B6@lsexch.lsmaster.lifespan.org> Storage of tissue in alcohol won't affect the presence or reactivity of aluminum. Formalin should not have a deleterious effect either, if it is properly buffered. I believe long-term storage in acidic formalin (or any acidic solution) might dissolve aluminum out of tissue, though I don't have any reference or direct experience to corroborate this. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Lina > Vieira > Sent: Thursday, March 30, 2006 11:11 AM > To: Histonet > Subject: [Histonet] Aluminium localization > > In return to the Aluminium localization, > I would now if an excessive time of fixation (in formaldeide), or an > excessive time of etanol 70% imersion (for example 4 days)can induce false > negative results to the aluminum? > > > Thanks in advance for any information you can provide, > > Lina Vieira > University of Algarve > Portugal > > _______________________________________________ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From MVaughan4 <@t> ucok.edu Thu Mar 30 14:19:37 2006 From: MVaughan4 <@t> ucok.edu (MVaughan4@ucok.edu) Date: Thu Mar 30 14:20:07 2006 Subject: [Histonet] Good used microtome for sale? In-Reply-To: Message-ID: Histonetters, I might be looking for a good used microtome in the next few months (provided the grant comes in). I have a good working AO820 from the 60's? but it has a lot of trouble sectioning anything less than 8 microns thick, and I don't have one of those newer disposable blade holders either. I will be glad to pay a reasonable price for it. Thanks. Mel Melville B. Vaughan, Ph. D. Assistant Professor Department of Biology University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm From JosefaNava <@t> texashealth.org Thu Mar 30 14:47:20 2006 From: JosefaNava <@t> texashealth.org (Nava, Josefa) Date: Thu Mar 30 14:48:04 2006 Subject: [Histonet] multi tissue block for keratin Message-ID: <2C515C1049EAF5459EFD8C9B929078A419463E@phdex03.txhealth.org> Hello Everyone, I need some suggestions please on what kind of tissues do I need to put together to build a multi control tissue for different subtypes of Keratin? Any additional information you can provide me is greatly appreciated on how to go about creating a multi tissue control block. Thank you so much. Josie Nava The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From jwatson <@t> gnf.org Thu Mar 30 14:51:12 2006 From: jwatson <@t> gnf.org (James Watson) Date: Thu Mar 30 14:51:20 2006 Subject: [Histonet] Marker for mouse vs human tissue Message-ID: I am looking for an antibody to differentiate human cells from mouse tissue in human implants in mouse. I have tried to locate a human mitochondrial marker, but have only been able to find it made in mouse. Any information would be appreciate. Thank you. James Watson HT, ASCP Facilities Manager of Histology GNF, Genomics Institute of the Novartis Research Foundation Room C015 858-332-4647 jwatson@gnf.org From kcai <@t> prosci-inc.com Thu Mar 30 16:13:21 2006 From: kcai <@t> prosci-inc.com (karen Cai) Date: Thu Mar 30 16:10:40 2006 Subject: [Histonet] paraffin tissue embedding and cutting Message-ID: <000e01c65447$288b0b10$7d01a8c0@prosci.com> Hello there, I have some questions regarding embedding and cutting paraffin tissue block: =20 1. After embedding, I found there are a lot of bubbles inside the paraffin tissue block (I put the mold at RT). Sometimes it has, sometimes not. I don=92t know why. How to avoid this? 2. Sometimes I found if I put the paraffin tissue sections into the 37C water bath, the paraffin will be diffused very quickly. Also the color of the whole paraffin tissue block look strange: white, not clear white. I am jus wondering whether the method of embedding tissue is wrong, or something else? =20 Any kind of help will be very appreciated, =20 Thanks in advance, =20 Best, Karen =20 =20 =20 --=20 No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.1.385 / Virus Database: 268.3.3/298 - Release Date: 3/30/2006 =20 From gcallis <@t> montana.edu Thu Mar 30 16:51:19 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Mar 30 16:51:27 2006 Subject: air filling Re: [Histonet] Frozen embedding of lungs In-Reply-To: <442B62ED.10302@uwo.ca> References: <130.194.113.116.1143679180@my.monash.edu.au> <442B62ED.10302@uwo.ca> Message-ID: <6.0.0.22.1.20060330121047.01b53bc0@gemini.msu.montana.edu> More on snap freezing murine lungs. and long discussion. Air filling a lung is not going to help with support during cryomicrotomy. The OCT must be in close contact with inner lung surfaces i.e replace the air, to ensure lung cryosections correctly. If not, air filled or partially air filled lungs (normally what you end up after a dissection and snap freezing, and what Kim was experiencing) still cryosections like a crumbling, overhanging sand dune. Air filling is really no different than taking a lung from an animal who has breathed and although the lung is collapsed enough air remains to give the crumbling section syndrome even after proper snap freezing. We have experienced this too many times until we performed OCT filling of the lung to replace the air. Lung morphology in our hands is intact and excellent. Some individuals fill or perfuse a lung with formalin or paraformaldehyde. Fixed lungs should be cryoprotected with 30% sucrose to replace the water (in the fixative ) to prevent large water ice crystal formation. Slow freezing creates the same problem by large water ice crystal formation in ANY fresh tissue. Water seems to be the enemy here. It is very important to snap freeze OCT filled then OCT surrounded/embedded tissue with a proper snap freezing setup as John described and never use a cryostat freezing apparatus that cannot achieve a cold enough temperature. We embed a whole lung in large plastic cryomolds, then snap freeze in a dry ice/isopentane or hexane mixture at approx -90C to have complete snap freezing of OCT filled lung in about 10 seconds or so. Liquid nitrogen cooled isopentane is just another method. That rapid freezing is critical for prevention of freezing artifact. One could choose to freeze the OCT filled lung without embedding, but Dodds taught us to coat fresh bone with a water soluble 70,000 PVA before immersing into a dry ice/hexane slurry. The MW weight of this PVA means the PVA molecule is too large for proper infiltration into tissue micro-spaces. Our Center for Biofilm Engineering indicated the PVA should be MW of 40,000 or less for infiltration purposes. For the tissue coating method, we dilute OCT (since it already contains PVA) with PBS (1:1) and dip the fresh tissue, OCT filled lung, or bone in this mixture prior to snap freezing. It could be assumed this thin OCT layer protects the tissue during -80C storage and maybe from direct contact with the hexane or isopentane although these may not affect the tissue in any way. OCT bottle has all ingredients stated clearly. OCT contains 10.24% w/w polyvinyl alcohol ,4.26% polyethylene glycol (the MW of PVA and PEG is not given for proprietary reasons) and 85.50% non-reacative ingredients which could be water. Dr. McCormick developed OCT for Tissue Tek many years ago and per private conversation he said could vary the ingredients for any given cryotomy temperature desired - very clever! The lung is NOT cryoprotected with OCT as that is not the purpose of this embedding media. In order to cryoprotect, an ingredient i.e.30% sucrose, should infiltrate into tissue micro-spaces to replace water. The MW of the PVA and PEG in OCT may be too great to achieve proper infiltration into tiny tissue spaces., hence the word 'surround'. It has been observed that some say they "infiltrate" with OCT by letting tissue sit overnight - and it may actually be more the OCT oozes into the irregular tissue spaces unless the space is big enough to let a large molecule in? Some mix OCT with 20% sucrose (1:1 mixture) and let this sit. The sucrose is probably what infiltrates the tissue spaces to replace water and NOT the PVA of PEG of the OCT. It would be an interesting experiment to take Thermo Electrons colored cryoembedding media and see if there is any infiltration into the tissue spaces - a line of green or blue in the tissue? The OCT bottle says this media is to "bind tissue to the specimen block and to surround and cover the tissue specimen". I interpret this as meaning NOT an infiltration medium like hot paraffin, but only as a means of supporting (air really doesn't do this very well) by direct contact with tissue surfaces for cryomicrotomy at optimal cutting temperature (we use it in a temperature range from -15C to -35C. If my thoughts are incorrect, hopefully Dr. McCormick is looking in and will make further comments. Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 At 09:47 PM 3/29/2006, you wrote: >Why not inflate with air (Pasteur pipette in >trachea) and then plunge into isopentane (cooled >by liquid nitrogen) or acetone (with dry ice ion >it). Neither is ideal, but both freezing methods >protect quite well against ice crystal damage; the >crystal holes are much smaller than cells. > >Coating a specimen with goop before freezing >ensures that the cells of the specimen are the >last and most slowly frozen things in the blob. >That makes for maximum damage by ice crystal >formation. With slow freezing most of the the ice >crystal holes are about the same size as a cell, >and the tissue architecture is 100% wrecked. >Unfixed objects must be frozen alost instantly. A >blob of hydrophilic polymer can be added >afterwards to protect against freeze-drying of >stored specimen. OCT (whatever it is; ?polyvinyl >alcohol) is a physical support for specimens being >sectioned with a cryostat or an olde-worlde >freezing microtome. It does not infiltrate the >tissue in the way paraffin wax enters every >dehydrated crevice. Most of the functions of OCT >are duplicated by other hydrohilic polymers such >as gelatin or agar. > >The abbreviation OCT is for Optimum Cutting >Temperature. That has nothing to do with >protection against freezing artifacts. It relates >to the hardness of the polymer at some temperature >that was, many years ago, considered optimal for >cutting frozen sections. > >John Kiernan >London, Canada >----------------------- > >Kim O'Sullivan wrote: >>Hi all, >>Does any one out there have a successful method for inflating mouse lungs >>to freeze. We currently freeze them unfixed in OCT, but of course the >>morphology is shocking as the lungs have collapsed. Is there a method >>that preserves the morphololgy without collapsing or imploding the lungs. >>Any advice will be appreciated >>Kim O'Sullivan >>Centre for Inflammatory Diseases >>Department of Medicine >>Monash University >>Melbourne >>Australia From gcallis <@t> montana.edu Thu Mar 30 17:01:53 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Mar 30 17:02:02 2006 Subject: [Histonet] insect histology book inquiry In-Reply-To: <4.3.2.7.2.20060330081133.00cdc960@algranth.inbox.email.ari zona.edu> References: <4.3.2.7.2.20060330081133.00cdc960@algranth.inbox.email.arizona.edu> Message-ID: <6.0.0.22.1.20060330160024.01b6f3f0@gemini.msu.montana.edu> Try to access the book via interlibrary loan with your university. We did this successfully with a very old book, long out of print. At 08:18 AM 3/30/2006, you wrote: >Good Thursday morning! >Is there anybody out there in histoland who might have a copy of the now >out of print book, A Manual of Basic Techniques in Insect Histology by P. >Barbosa, Autumn Publishers, 1974? Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 From wangche <@t> auburn.edu Thu Mar 30 22:48:42 2006 From: wangche <@t> auburn.edu (Chengming Wang) Date: Thu Mar 30 22:48:48 2006 Subject: [Histonet] Help needed!!! Immunofluorescence double staining ofwith mouse lung Message-ID: <442C604A020000AF0001A6EE@groupwise1.duc.auburn.edu> Dear ALL, This is Chengming from Auburn University, and this is my first time here, and I need your help with my frustrating double staining of immunofluorescence with mouse lung. This is cryosection of mouse lung, only fixed with 5-minute acetone without involvement of formalin or paraffin. I am trying to stain two targets with Alexa Fluro 488 and Alexa Fluro 594, and counter stain with DAPI. The individual staining works fine. However, I found that: when I stained with 2nd antibody of Alexa 594, I could see some signals in FITC channel (Alex 488). And this signal from Alexa 594 to 488 really bothers me when we performed double staining. I tried that the autofluorescence is very very weak in all cases. In reading the distributions of different fluorescence dyes, there should not be any signal spillovers from Alex 594 to 488. Any help or suggestions are highly appreciated!!! If you have experience with this, please let me know! Thanks in advance! Chengming Wang Auburn University From kemlo.rogerson <@t> waht.swest.nhs.uk Fri Mar 31 01:25:31 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Mar 31 01:24:52 2006 Subject: [Histonet] paraffin tissue embedding and cutting Message-ID: Either xylene contamination or wax contaminated. I have noticed if you use freshly melted wax then you get the bubbles, you may wish to put it in a vacuum to lower SVP which ought to 'draw out' the bubbles. Sometimes you find that the wax melts on the water bath as if its too hot; if the bath is at the optimum temperature then look at xylene contamination or something 'wrong' with the wax. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 "Following the line of least resistance makes both men and rivers crooked" This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: karen Cai [mailto:kcai@prosci-inc.com] Sent: Thursday, March 30, 2006 11:13 PM To: HISTONET@LISTS.UTSOUTHWESTERN.EDU Subject: [Histonet] paraffin tissue embedding and cutting Hello there, I have some questions regarding embedding and cutting paraffin tissue block: 1. After embedding, I found there are a lot of bubbles inside the paraffin tissue block (I put the mold at RT). Sometimes it has, sometimes not. I don't know why. How to avoid this? 2. Sometimes I found if I put the paraffin tissue sections into the 37C water bath, the paraffin will be diffused very quickly. Also the color of the whole paraffin tissue block look strange: white, not clear white. I am jus wondering whether the method of embedding tissue is wrong, or something else? Any kind of help will be very appreciated, Thanks in advance, Best, Karen -- No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.1.385 / Virus Database: 268.3.3/298 - Release Date: 3/30/2006 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo.rogerson <@t> waht.swest.nhs.uk Fri Mar 31 01:51:41 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Mar 31 01:50:58 2006 Subject: [Histonet] Breast core biopsies, same day results by frozen s ections Message-ID: Its also dangerous to make the wrong diagnosis, maybe back then patient's were not so litigatious. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 "Following the line of least resistance makes both men and rivers crooked" This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation -----Original Message----- From: Ford Royer [mailto:froyer@bitstream.net] Sent: Thursday, March 30, 2006 4:45 PM To: HISTONET@LISTS.UTSOUTHWESTERN.EDU Subject: RE: [Histonet] Breast core biopsies, same day results by frozen sections WOW... have times changed(?). In my day (1970s), the SOP was to do STAT frozen sections on breast biopsies right in the surgery suite while the patient was still under anesthesia. The rationale was that if the biopsy was positive, they could continue with the mastectomy while the pt. was still under. The feeling (that I was told at the time) was that it was too dangerous to put the patient under general anesthesia twice; once for the biopsy, and then again a few days later for the mastectomy. Of course back then, they felt that if you extracted all the patient's teeth that it would cure their arthritis, and of course frontal lobotomies where state of the art for those with schizophrenia. ~ Ford Ford M. Royer, MT(ASCP) Minnesota Medical Specialists, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 763-542-8725 phone 888-790-9686 toll free 763-546-4830 fax email web -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles.Embrey Sent: Thursday, March 30, 2006 8:17 AM To: Galiotto, Laura; HISTONET@LISTS.UTSOUTHWESTERN.EDU Subject: RE: [Histonet] Breast core biopsies,same day results by frozen sections What a scary thought. The first real question is: What would be the benefit? Are you going to do the lumpectomy or mastectomy immediately following the frozen section result? Fat is difficult to cut on frozen and I shudder to think of all the wasted (and now non-diagnosable) tissue you will incur trying to get an adequate section. To me, a frozen section is only of value if it will directly impact a surgical decision that will result in immediate action. The biopsy is a unique specimen and even if you were to attempt to get more tissue you can't be certain that it will yield the same result. I can't see possibly wasting it on an unnecessary frozen section. Charles Embrey PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Galiotto, Laura Sent: Thursday, March 30, 2006 7:40 AM To: HISTONET@LISTS.UTSOUTHWESTERN.EDU Subject: [Histonet] Breast core biopsies, same day results by frozen sections Is there anyone performing same day results on breast biopsies by frozen sections? If so please share the pros and cons. Has there been any false negative results? Laura Galiotto, HT (ASCP) Histology Facilitator Northwest Community Hospital 800 West Central Road Arlington Heights, Il 60005 847-618-6190 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GauchV <@t> mail.amc.edu Fri Mar 31 07:48:58 2006 From: GauchV <@t> mail.amc.edu (Vicki Gauch) Date: Fri Mar 31 07:48:47 2006 Subject: [Histonet] Disposal of specimens/formalin Message-ID: We have been recently informed that our method of disposal for our formalin filled containers will have to be changed. Currently we dispose of the filled containers (specimens and formalin) in double red biohazard bags which are then put in hardfiber boxes. I would be very interested in how other institutions are handling their waste disposal. Is anyone separating the formalin from the tissues in the containers prior to discard and if so, what type of procedure are they following to accomplish this? Any help would be greatly appreciated since we will have to implement some plan sooner rather than later. Thank you, Vicki AMCH ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. From Susan.Ferrigon <@t> sanofi-aventis.com Fri Mar 31 07:55:41 2006 From: Susan.Ferrigon <@t> sanofi-aventis.com (Susan.Ferrigon@sanofi-aventis.com) Date: Fri Mar 31 07:55:48 2006 Subject: [Histonet] DAB Message-ID: Hi Does anyone have a method or any experience on Nickel enhancement for DAB. Any advice would be greatly appreciated. Thanks Susan ------------------------------ Le pr?sent message ainsi que ses ?ventuelles pi?ces jointes est exclusivement destin? au(x) destinataire(s), personnes physiques ou morales, qu'il d?signe. Il constitue de ce fait une correspondance ? caract?re priv? et peut contenir des informations confidentielles. Si ce message vous est parvenu par erreur, nous vous remercions d'en aviser imm?diatement l'exp?diteur par retour de ce courrier ?lectronique puis de le d?truire, ainsi que ses ?ventuellement pi?ces jointes, sans en conserver de copie. This message, including any attachment, is intended for the use of the individual or entity to which it is addressed. It is therefore to be considered as a private correspondence which may contain confidential information. If you are not the intended recipient, please advise the sender immediately by reply e.mail and delete this message and any attachment thereto without retaining a copy. ------------------------------ From Susan.Ferrigon <@t> sanofi-aventis.com Fri Mar 31 08:04:18 2006 From: Susan.Ferrigon <@t> sanofi-aventis.com (Susan.Ferrigon@sanofi-aventis.com) Date: Fri Mar 31 08:04:30 2006 Subject: [Histonet] F4/ 80 Message-ID: Hi Thanks to everyone who have helped me so far with the problems I am having with my f4/80, however I have tried lots of the different bits of advise and still seem to be getting the same results, so I wondered if any one with lots of experience in f4/80 would be willing to let me have their email address so I can send some pictures to look at and I will send the protocol I am using and perhaps you could see where I going wrong. Thanks in advance Susan ------------------------------ Le pr?sent message ainsi que ses ?ventuelles pi?ces jointes est exclusivement destin? au(x) destinataire(s), personnes physiques ou morales, qu'il d?signe. Il constitue de ce fait une correspondance ? caract?re priv? et peut contenir des informations confidentielles. Si ce message vous est parvenu par erreur, nous vous remercions d'en aviser imm?diatement l'exp?diteur par retour de ce courrier ?lectronique puis de le d?truire, ainsi que ses ?ventuellement pi?ces jointes, sans en conserver de copie. This message, including any attachment, is intended for the use of the individual or entity to which it is addressed. It is therefore to be considered as a private correspondence which may contain confidential information. If you are not the intended recipient, please advise the sender immediately by reply e.mail and delete this message and any attachment thereto without retaining a copy. ------------------------------ From gpbnas <@t> yahoo.es Fri Mar 31 09:06:17 2006 From: gpbnas <@t> yahoo.es (Guillermo Palao) Date: Fri Mar 31 09:06:25 2006 Subject: [Histonet] RE: Double staining In-Reply-To: Message-ID: <20060331150617.44281.qmail@web26203.mail.ukl.yahoo.com> My two cents is just to follow Andrea's advice on using pre-adsorbed secondaries (I also use Jackson's with great results), it will really save you a lot of time wasted on optimizating conditions. Guillermo "Andrea T. Hooper" escribi?: Hi Anna, Your experiment will work! We do this all the time here with very nice results and it also sounds like you know what you are talking about. I suggest you check to make sure the secondaries are highly cross adsorbed and if not, buy ones which are (Jackson ImmunoResearch sells awesome secondaries). I also strongly suggest you run each independently to make sure everything is in order. I normally do this alongside my double stains each time. Also as Chris suggested you can even incubate your primaries together making your experiment even additionally streamlined. And of course remember to block appropriately. Good luck, Andrea >Anna, >I am very interested in this. >I am not a chemist, so I would welcome more educated predictions. >But theoretically, this secondary cocktail MIGHT work. My exposure to >this is very limited and is based on my experience with the Biocare >doublestain secondary cocktails. >You would need to get more information on the actual chemistry of the >secondary components...would the flourophore "smother" out the other >secondary? I would question how the biotinylated component would cross >react with the flourophore...I have no direct experience to this. >Then there's the dilution of each when they are placed together... >I see the main question is how well the flourophore can cohabitate with >your biotinylated secondary > >Have you tried each stain separately? >Then doing the double stain the "traditional" way? > >If you could get a base line, that might help to see what happens when >you start making the voodoo brew. > >Let me know how this goes! > > Becky Orr CLA,HT(ASCP) >IHC Lead >Evanston Northwestern Healthcare >847-570-2771 > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- LLama Gratis a cualquier PC del Mundo. Llamadas a fijos y m?viles desde 1 c?ntimo por minuto. http://es.voice.yahoo.com From gcallis <@t> montana.edu Fri Mar 31 09:24:47 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Mar 31 09:24:55 2006 Subject: [Histonet] Disposal of specimens/formalin In-Reply-To: References: Message-ID: <6.0.0.22.1.20060331082324.01b50988@gemini.msu.montana.edu> If shipping it becomes very expensive or some collection agency/lab has to do this at great expense, consider formalin recycling. I know of labs who use this and love it, it cut down on costs tremendously. At 06:48 AM 3/31/2006, you wrote: >We have been recently informed that our method of disposal for our >formalin filled containers will have to be changed. Currently we >dispose of the filled containers (specimens and formalin) in double red >biohazard bags which are then put in hardfiber boxes. I would be very >interested in how other institutions are handling their waste disposal. >Is anyone separating the formalin from the tissues in the containers >prior to discard and if so, what type of procedure are they following to >accomplish this? Any help would be greatly appreciated since we will >have to implement some plan sooner rather than later. >Thank you, > Vicki >AMCH > >----------------------------------------- >CONFIDENTIALITY NOTICE: This email and any attachments may contain >confidential information that is protected by law and is for the >sole use of the individuals or entities to which it is addressed. >If you are not the intended recipient, please notify the sender by >replying to this email and destroying all copies of the >communication and attachments. Further use, disclosure, copying, >distribution of, or reliance upon the contents of this email and >attachments is strictly prohibited. To contact Albany Medical >Center, or for a copy of our privacy practices, please visit us on >the Internet at www.amc.edu. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 From tkngflght <@t> yahoo.com Fri Mar 31 09:32:15 2006 From: tkngflght <@t> yahoo.com (Cheryl) Date: Fri Mar 31 09:32:22 2006 Subject: [Histonet] job opening: cool location--with a nice addition of temp to perm. Message-ID: <20060331153215.95634.qmail@web50906.mail.yahoo.com> Good Morning Histofolk-- I don't usually solicit candidates on this forum but I've been given a new twist on a job that I'D take if I could leave my current location.... Have you ever wanted to live in Alaska but were concerned once you got there you'd not like it and couldn't change your mind??? Here is your chance to give it a go--risk-free. I'm looking for an experienced tech--5+ years--for a GOOD and HAPPY lab in one of the larger cities in The Last Frontier. This is NOT a remote location, is an established, well-equipped lab, and they'd like to give you the chance to try it on for size before moving your life and family. You'd start as a temp employee of Full Staff and if you wanted to stay, would transfer to their employ with benies available on sign-on. Good relo and pay rates--they want to pay you MORE than midrange. If it didn't work out but you did a good job while there, you could continue as a temp on my roles or we'd work to find you another perm in the lower 48. If you are even JUST CURIOUS, please give me a call. DO NOT RESPOND ON THIS LIST---write to me at my business email or call-- admin@fullstaff.org 281.852.9457 o/f 281.883.8804 c Thanks for taking the time to read--if you refer someone for this, referral bonuses of $300 apply!! I'm always looking for travelers and help with permanent job placement (the focus of Full Staff) so any inquiries and referrals are welcome. Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one Tech at a time. 281.883.7704 c 281.852.9457 o admin@fullstaff.org Sign up for the FREE monthly newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. From tracy.bergeron <@t> crl.com Fri Mar 31 09:53:54 2006 From: tracy.bergeron <@t> crl.com (tracy.bergeron@crl.com) Date: Fri Mar 31 09:54:32 2006 Subject: [Histonet] Disposal of specimens/formalin In-Reply-To: <6.0.0.22.1.20060331082324.01b50988@gemini.msu.montana.edu> Message-ID: Using Recycling can be tricky if your lab does GLP work. I work in a research laboratory that does doe GLP work, and in part for that reason, we do not use any sort of recycled reagents. This means of course we produce a fairly large amount of waste that has to be removed regularly. For formalin and tissue disposal. We have a fume hood that has a large hole in the counter where a funnel which is attached to a 30 gallon drum sits. This way all tissue and formalin dumping is done in the fume hood, which reduces any possible exposure to formalin, and formaldehyde. We empty/ drain the formalin out of the cups or bags into the funnel. The tissue is put into an autoclavable bag. The bag is then sealed and put in the carcass freezer. That freezer is emptied on a daily basis, the bags are put into the hard fiber boxes then taken away. I believe our biowaste is incinerated. The formalin along with all other hazardous waste, is taken away on a regular basis by Onyx (http://www.onyx-environment.com). The histo lab at our facility is the largest generator of Haz Waste, though we have not made it to a high volume generator status, and are hoping to stay below it. I do not know the specifics on I hope this info is of some help. Tracy E. Bergeron, BS, HT (ASCP) Histotechnician Charles River Laboratories Wilmington, MA 978-658-6000 x 1229 Gayle Callis Sent by: histonet-bounces@lists.utsouthwestern.edu 03/31/2006 10:24 AM To "Vicki Gauch" , Histonet@lists.utsouthwestern.edu cc Subject Re: [Histonet] Disposal of specimens/formalin If shipping it becomes very expensive or some collection agency/lab has to do this at great expense, consider formalin recycling. I know of labs who use this and love it, it cut down on costs tremendously. At 06:48 AM 3/31/2006, you wrote: >We have been recently informed that our method of disposal for our >formalin filled containers will have to be changed. Currently we >dispose of the filled containers (specimens and formalin) in double red >biohazard bags which are then put in hardfiber boxes. I would be very >interested in how other institutions are handling their waste disposal. >Is anyone separating the formalin from the tissues in the containers >prior to discard and if so, what type of procedure are they following to >accomplish this? Any help would be greatly appreciated since we will >have to implement some plan sooner rather than later. >Thank you, > Vicki >AMCH > >----------------------------------------- >CONFIDENTIALITY NOTICE: This email and any attachments may contain >confidential information that is protected by law and is for the >sole use of the individuals or entities to which it is addressed. >If you are not the intended recipient, please notify the sender by >replying to this email and destroying all copies of the >communication and attachments. Further use, disclosure, copying, >distribution of, or reliance upon the contents of this email and >attachments is strictly prohibited. To contact Albany Medical >Center, or for a copy of our privacy practices, please visit us on >the Internet at www.amc.edu. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Fri Mar 31 09:58:51 2006 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri Mar 31 09:58:55 2006 Subject: [Histonet] microwave technology advance Message-ID: Has anyone any experience of the new laboratory microwave from APRUNO DIAGNOSTICS??; which not only has a processing and staining facility but also includes a limited diagnostic capability. Any feedback gratefully received. Thanks O.N.De-Frost.....MD.DCM.DSTP. From JWEEMS <@t> sjha.org Fri Mar 31 10:08:57 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Mar 31 10:09:00 2006 Subject: [Histonet] Disposal of specimens/formalin Message-ID: <83AACDB0810528418AA106F9AE9B7F7E01D34F10@sjhaexc02.sjha.org> We discard our smaller containers this way, but add formalin neutralizer to the bag. For the larger containers we separate the tissue from the formalin and then neutralize the formalin. We have on site disposal. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Vicki Gauch Sent: Friday, March 31, 2006 8:49 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Disposal of specimens/formalin We have been recently informed that our method of disposal for our formalin filled containers will have to be changed. Currently we dispose of the filled containers (specimens and formalin) in double red biohazard bags which are then put in hardfiber boxes. I would be very interested in how other institutions are handling their waste disposal. Is anyone separating the formalin from the tissues in the containers prior to discard and if so, what type of procedure are they following to accomplish this? Any help would be greatly appreciated since we will have to implement some plan sooner rather than later. Thank you, Vicki AMCH ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From leslie.duncan <@t> bms.com Fri Mar 31 10:11:44 2006 From: leslie.duncan <@t> bms.com (Leslie D Duncan) Date: Fri Mar 31 10:13:47 2006 Subject: [Histonet] Disposal of specimens/formalin In-Reply-To: References: Message-ID: <442D54C0.4080809@bms.com> Hi Vicki, Our formalin waste is picked up by Onyx also - they provide containers to collect the waste in, and pick it up on a regular basis (depending on your lab needs). I have no idea the cost, but they collect all of our hazardous waste including formic acid, bouins, alcohol, xylene, all expired chemicals/reagents that cannot be disposed of down the drain. Best of luck, Leslie Vicki Gauch wrote: >We have been recently informed that our method of disposal for our >formalin filled containers will have to be changed. Currently we >dispose of the filled containers (specimens and formalin) in double red >biohazard bags which are then put in hardfiber boxes. I would be very >interested in how other institutions are handling their waste disposal. >Is anyone separating the formalin from the tissues in the containers >prior to discard and if so, what type of procedure are they following to >accomplish this? Any help would be greatly appreciated since we will >have to implement some plan sooner rather than later. >Thank you, > Vicki >AMCH > >----------------------------------------- >CONFIDENTIALITY NOTICE: This email and any attachments may contain >confidential information that is protected by law and is for the >sole use of the individuals or entities to which it is addressed. >If you are not the intended recipient, please notify the sender by >replying to this email and destroying all copies of the >communication and attachments. Further use, disclosure, copying, >distribution of, or reliance upon the contents of this email and >attachments is strictly prohibited. To contact Albany Medical >Center, or for a copy of our privacy practices, please visit us on >the Internet at www.amc.edu. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From PIXLEYSK <@t> UCMAIL.UC.EDU Fri Mar 31 10:19:13 2006 From: PIXLEYSK <@t> UCMAIL.UC.EDU (Pixley, Sarah (pixleysk)) Date: Fri Mar 31 10:19:17 2006 Subject: [Histonet] RE: Dab enhancement Message-ID: Dear Susan: There are several ways to enhance. We have found the simplest for us is: Nickel enhancement of DAB: DAB (0.5 mg/ml) with 0.08% nickel sulfate and 3% H2O2: Volume needed: 1 ml 2% nickel sulfate 40 ul DAB Solution 1 ml 30% H2O2 10 ul To make: 2% Nickel sulfate, NiSO4.6H2O: 0.9 g into 45 ml E-pure dd H2O = 2% solution. Keep in Fridge. (Dilute: 1/25 to give a 0.08% solution.) NOTE: time to getting staining will be much quicker than without nickel. And this solution develops more precipitate than a non-nickel solution, so if you are doing a lengthy DAB staining because you have many samples, you may want to plan to make more DAB solution in the middle of the staining. Another easy trick is to treat your sections with cupric sulfate after staining (does not seem to be as dark or enhancing as adding nickel with the DAB--more brown than blue-black). Post-DAB enhancement 0.4% cupric sulfate (CuSO4?5H2O) In 0.9% NaCl Keep cupric sulfate at room temp. in closed glass bottle (good for many years). After DAB reaction has been completed and rinsed well with PBS or PB, immerse slides for short time, i.e. 5-20 mins, and then rinse with PBS, saline or water. Darkens regular DAB color to blacker shade. From rjbuesa <@t> yahoo.com Fri Mar 31 10:49:38 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Mar 31 10:49:43 2006 Subject: [Histonet] DAB In-Reply-To: Message-ID: <20060331164938.57675.qmail@web61223.mail.yahoo.com> Hi Susan: I always used nickel salts when I wanted to detect 2 different epitopes in the same section. One I detected with regular DAB to get the "classical" permanent brown-reddish color. To detect the second epitope I used a DAB modification with nickel salts that will give an also permanent signal, but this time "blue-purplish" in a way that I could visualize both in the same section. I prepared the second chromogen as follows: a- DAB ---- 6 mg b- PBS ---- 10 mL c- 3% H2O2 --- 160 ?L d- 16.2% aq. solution of nickel sulfate hexahydrated --- 120 ?L Mix like the regular DAB and apply to the section an let act for 6 minutes. I hope this will help you. Ren? J. Susan.Ferrigon@sanofi-aventis.com wrote: Hi Does anyone have a method or any experience on Nickel enhancement for DAB. Any advice would be greatly appreciated. Thanks Susan ------------------------------ Le pr?sent message ainsi que ses ?ventuelles pi?ces jointes est exclusivement destin? au(x) destinataire(s), personnes physiques ou morales, qu'il d?signe. Il constitue de ce fait une correspondance ? caract?re priv? et peut contenir des informations confidentielles. Si ce message vous est parvenu par erreur, nous vous remercions d'en aviser imm?diatement l'exp?diteur par retour de ce courrier ?lectronique puis de le d?truire, ainsi que ses ?ventuellement pi?ces jointes, sans en conserver de copie. This message, including any attachment, is intended for the use of the individual or entity to which it is addressed. It is therefore to be considered as a private correspondence which may contain confidential information. If you are not the intended recipient, please advise the sender immediately by reply e.mail and delete this message and any attachment thereto without retaining a copy. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- New Yahoo! Messenger with Voice. Call regular phones from your PC for low, low rates. From billingconsultants <@t> yahoo.com Fri Mar 31 10:56:52 2006 From: billingconsultants <@t> yahoo.com (Billing Consultants, LLC) Date: Fri Mar 31 10:56:58 2006 Subject: [Histonet] Attn: Vendors Message-ID: <20060331165652.37858.qmail@web54209.mail.yahoo.com> Hi everyone, A client is interested in purchasing a used H&E x-y stainer as well as looking into both used and new IHC stainers. Any information you have would be greatly appreciated. Louri Roberts histonet-request@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: CAP Immuno Survey Slides (Richard Cartun) 2. RE: CAP Immuno Survey Slides (Dana Settembre) 3. Aluminium localization (Lina Vieira) 4. RE: CAP Immuno Survey Slides (Dawson, Glen) 5. Re: Health and Safety Guidelines for the Lab by L Montgomery needed (jason.burrill@crl.com) 6. eosin Y (Jennifer MacDonald) 7. RE: Aluminium localization (Monfils, Paul) 8. Good used microtome for sale? (MVaughan4@ucok.edu) 9. multi tissue block for keratin (Nava, Josefa) 10. Marker for mouse vs human tissue (James Watson) 11. paraffin tissue embedding and cutting (karen Cai) 12. air filling Re: [Histonet] Frozen embedding of lungs (Gayle Callis) 13. Re: insect histology book inquiry (Gayle Callis) 14. Help needed!!! Immunofluorescence double staining ofwith mouse lung (Chengming Wang) 15. RE: paraffin tissue embedding and cutting (Kemlo Rogerson) 16. RE: Breast core biopsies, same day results by frozen s ections (Kemlo Rogerson) 17. Disposal of specimens/formalin (Vicki Gauch) 18. DAB (Susan.Ferrigon@sanofi-aventis.com) 19. F4/ 80 (Susan.Ferrigon@sanofi-aventis.com) 20. RE: RE: Double staining (Guillermo Palao) 21. Re: Disposal of specimens/formalin (Gayle Callis) 22. job opening: cool location--with a nice addition of temp to perm. (Cheryl) 23. Re: Disposal of specimens/formalin (tracy.bergeron@crl.com) 24. microwave technology advance (Edwards, R.E.) ---------------------------------------------------------------------- Message: 1 Date: Thu, 30 Mar 2006 12:59:26 -0500 From: "Richard Cartun" Subject: RE: [Histonet] CAP Immuno Survey Slides To: "JUAN GUTIERREZ" , "Histonet (E-mail)" , "Laura Jones" Message-ID: Content-Type: text/plain; charset=US-ASCII I agree. I will forward this to a member of the CAP Cell Markers Committee. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "GUTIERREZ, JUAN" 03/30/06 12:44PM >>> We pulled the labels off. What a pain in the .... To: CAP, please don't do that again. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jones, Laura Sent: Thursday, March 30, 2006 11:05 AM To: Histonet (E-mail) Subject: [Histonet] CAP Immuno Survey Slides Hello Histoland. We were wondering if anyone else who may have participated in the recent CAP survey had experienced any problems with the labels on the slides. We performed some of the survey by hand (our machine is down!) using the Shandon Sequenza coverplates, and it seemed like the label adhesive was blocking the reagents from passing onto the slide. We then repeated some of the slides on a demo machine, after verifying our antibodies, and even then it seemed like that adhesive was interfering with the reagents. We also lost any info we had written on the slide labels, even with the impervious markers, during automated processing. We didn't remember our survey slides ever having labels before, and are just curious if anyone else had encountered this. Thanks in advance! The Histochicks at Sharon Regional, Sharon, PA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Thu, 30 Mar 2006 13:39:30 -0500 From: "Dana Settembre" Subject: RE: [Histonet] CAP Immuno Survey Slides To: "JUAN GUTIERREZ" , "Richard Cartun" , "Histonet (E-mail)" , "Laura Jones" Message-ID: Content-Type: text/plain; charset=US-ASCII Please do not send us slides with labels. They were really difficult to work with. Dana Settembre University Hospital - UMDNJ Newark, NJ >>> Richard Cartun 03/30/06 12:59 PM >>> I agree. I will forward this to a member of the CAP Cell Markers Committee. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "GUTIERREZ, JUAN" 03/30/06 12:44PM >>> We pulled the labels off. What a pain in the .... To: CAP, please don't do that again. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jones, Laura Sent: Thursday, March 30, 2006 11:05 AM To: Histonet (E-mail) Subject: [Histonet] CAP Immuno Survey Slides Hello Histoland. We were wondering if anyone else who may have participated in the recent CAP survey had experienced any problems with the labels on the slides. We performed some of the survey by hand (our machine is down!) using the Shandon Sequenza coverplates, and it seemed like the label adhesive was blocking the reagents from passing onto the slide. We then repeated some of the slides on a demo machine, after verifying our antibodies, and even then it seemed like that adhesive was interfering with the reagents. We also lost any info we had written on the slide labels, even with the impervious markers, during automated processing. We didn't remember our survey slides ever having labels before, and are just curious if anyone else had encountered this. Thanks in advance! The Histochicks at Sharon Regional, Sharon, PA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Thu, 30 Mar 2006 20:11:06 +0100 From: "Lina Vieira" Subject: [Histonet] Aluminium localization To: "Histonet" Message-ID: <007301c6542d$b28574f0$2914100a@labhistologia> Content-Type: text/plain; charset="iso-8859-1" In return to the Aluminium localization, I would now if an excessive time of fixation (in formaldeide), or an excessive time of etanol 70% imersion (for example 4 days)can induce false negative results to the aluminum? Thanks in advance for any information you can provide, Lina Vieira University of Algarve Portugal _______________________________________________ ------------------------------ Message: 4 Date: Thu, 30 Mar 2006 13:14:47 -0600 From: "Dawson, Glen" Subject: RE: [Histonet] CAP Immuno Survey Slides To: "Histonet \(E-mail\)" Message-ID: Content-Type: text/plain; charset="iso-8859-1" All, I've talked to the CAP about this issue and they said that they would "take it under advisement". Just goes to show you that those in charge of sending out these CAP surveys for IHC have no experience in the actual staining of IHC slides. Also, the labels they put on are heavy duty and the adhesive they leave behind is NOT condusive to automated coverslipping (with the Sakura Glass coverslipper). They tend to fall off the suction cup and get broken by the conveyor. I would strongly suggest coverslipping these slides by hand until the CAP decides to stop this craziness. Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Dana Settembre Sent: Thursday, March 30, 2006 12:40 PM To: JUAN GUTIERREZ; Richard Cartun; Histonet (E-mail); Laura Jones Subject: RE: [Histonet] CAP Immuno Survey Slides Please do not send us slides with labels. They were really difficult to work with. Dana Settembre University Hospital - UMDNJ Newark, NJ >>> Richard Cartun 03/30/06 12:59 PM >>> I agree. I will forward this to a member of the CAP Cell Markers Committee. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "GUTIERREZ, JUAN" 03/30/06 12:44PM >>> We pulled the labels off. What a pain in the .... To: CAP, please don't do that again. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jones, Laura Sent: Thursday, March 30, 2006 11:05 AM To: Histonet (E-mail) Subject: [Histonet] CAP Immuno Survey Slides Hello Histoland. We were wondering if anyone else who may have participated in the recent CAP survey had experienced any problems with the labels on the slides. We performed some of the survey by hand (our machine is down!) using the Shandon Sequenza coverplates, and it seemed like the label adhesive was blocking the reagents from passing onto the slide. We then repeated some of the slides on a demo machine, after verifying our antibodies, and even then it seemed like that adhesive was interfering with the reagents. We also lost any info we had written on the slide labels, even with the impervious markers, during automated processing. We didn't remember our survey slides ever having labels before, and are just curious if anyone else had encountered this. Thanks in advance! The Histochicks at Sharon Regional, Sharon, PA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Thu, 30 Mar 2006 14:22:19 -0500 From: jason.burrill@crl.com Subject: [Histonet] Re: Health and Safety Guidelines for the Lab by L Montgomery needed To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Thanks to all that responded and all your offers. Thanks to Victor Tobias I found a used copy at http://www.abebooks.com. It is a very difficult book to get your hands on. Jason Jason D. Burrill Manager, Histology Charles River Laboratories 251 Ballardvale Street Wilmington, MA 01887 ph: 978-658-6000 ext 1652 fax: 978-988-8793 E-mail: jason.burrill@crl.com ------------------------------ Message: 6 Date: Thu, 30 Mar 2006 11:25:29 -0800 From: Jennifer MacDonald Subject: [Histonet] eosin Y To: Melanie Black Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" What is the pH of the eosin? Melanie Black Sent by: histonet-bounces@lists.utsouthwestern.edu 03/30/2006 03:45 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] GMA & Humidity Dear All, I would like to ask about a particular problem with eosin-Y staining. I am using Eosin Y alcoholic without phloxine (from Sigma) and for some reason, all of the eosin staining comes out of the tissue as it is being dehydrated. I can actually see that it just drains out. I know that this is part of the differentiation process but it just all comes out. We use the same staining set for mouse liver tumour tissues with no problem. These are routinely processed (by a hospital pathology lab) rat rectum slides. Is the phloxine important for "keeping in the stain"? The sections are quite small. Should I be using a different eosin? Any advice on this matter would be greatly appreciated. Cathy Cathy JB 4 resin is one of the few resins that is water soluble, and complete dehydration of tissue is NOT required. In the processing of this resin, clearing agents like xylene & chloroform are unnecessary. I have used this resin many times, specially for tissue containing gels etc. Regards Melanie. -- Cardiovascular Research Unit Div. of Cardiothoracic Surgery Chris Barnard Building University of Cape Town Anzio Road Observatory 7925 Republic of South Africa Tel +27 21 406-6589 Cel +27 82 469-3352 Fax +27 21 448-5935 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Thu, 30 Mar 2006 14:45:14 -0500 From: "Monfils, Paul" Subject: RE: [Histonet] Aluminium localization To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <09C945920A6B654199F7A58A1D7D1FDE017176B6@lsexch.lsmaster.lifespan.org> Content-Type: text/plain; charset="iso-8859-1" Storage of tissue in alcohol won't affect the presence or reactivity of aluminum. Formalin should not have a deleterious effect either, if it is properly buffered. I believe long-term storage in acidic formalin (or any acidic solution) might dissolve aluminum out of tissue, though I don't have any reference or direct experience to corroborate this. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Lina > Vieira > Sent: Thursday, March 30, 2006 11:11 AM > To: Histonet > Subject: [Histonet] Aluminium localization > > In return to the Aluminium localization, > I would now if an excessive time of fixation (in formaldeide), or an > excessive time of etanol 70% imersion (for example 4 days)can induce false > negative results to the aluminum? > > > Thanks in advance for any information you can provide, > > Lina Vieira > University of Algarve > Portugal > > _______________________________________________ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 8 Date: Thu, 30 Mar 2006 14:19:37 -0600 From: MVaughan4@ucok.edu Subject: [Histonet] Good used microtome for sale? To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Histonetters, I might be looking for a good used microtome in the next few months (provided the grant comes in). I have a good working AO820 from the 60's? but it has a lot of trouble sectioning anything less than 8 microns thick, and I don't have one of those newer disposable blade holders either. I will be glad to pay a reasonable price for it. Thanks. Mel Melville B. Vaughan, Ph. D. Assistant Professor Department of Biology University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm ------------------------------ Message: 9 Date: Thu, 30 Mar 2006 14:47:20 -0600 From: "Nava, Josefa" Subject: [Histonet] multi tissue block for keratin To: Message-ID: <2C515C1049EAF5459EFD8C9B929078A419463E@phdex03.txhealth.org> Content-Type: text/plain; charset="us-ascii" Hello Everyone, I need some suggestions please on what kind of tissues do I need to put together to build a multi control tissue for different subtypes of Keratin? Any additional information you can provide me is greatly appreciated on how to go about creating a multi tissue control block. Thank you so much. Josie Nava The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. ------------------------------ Message: 10 Date: Thu, 30 Mar 2006 12:51:12 -0800 From: "James Watson" Subject: [Histonet] Marker for mouse vs human tissue To: Message-ID: Content-Type: text/plain; charset="us-ascii" I am looking for an antibody to differentiate human cells from mouse tissue in human implants in mouse. I have tried to locate a human mitochondrial marker, but have only been able to find it made in mouse. Any information would be appreciate. Thank you. James Watson HT, ASCP Facilities Manager of Histology GNF, Genomics Institute of the Novartis Research Foundation Room C015 858-332-4647 jwatson@gnf.org ------------------------------ Message: 11 Date: Thu, 30 Mar 2006 14:13:21 -0800 From: "karen Cai" Subject: [Histonet] paraffin tissue embedding and cutting To: Message-ID: <000e01c65447$288b0b10$7d01a8c0@prosci.com> Content-Type: text/plain; charset="windows-1250" Hello there, I have some questions regarding embedding and cutting paraffin tissue block: 1. After embedding, I found there are a lot of bubbles inside the paraffin tissue block (I put the mold at RT). Sometimes it has, sometimes not. I don?t know why. How to avoid this? 2. Sometimes I found if I put the paraffin tissue sections into the 37C water bath, the paraffin will be diffused very quickly. Also the color of the whole paraffin tissue block look strange: white, not clear white. I am jus wondering whether the method of embedding tissue is wrong, or something else? Any kind of help will be very appreciated, Thanks in advance, Best, Karen -- No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.1.385 / Virus Database: 268.3.3/298 - Release Date: 3/30/2006 ------------------------------ Message: 12 Date: Thu, 30 Mar 2006 15:51:19 -0700 From: Gayle Callis === message truncated === --------------------------------- New Yahoo! Messenger with Voice. Call regular phones from your PC and save big. From rajs05281998 <@t> yahoo.com Fri Mar 31 11:11:26 2006 From: rajs05281998 <@t> yahoo.com (suresh mathew) Date: Fri Mar 31 11:11:30 2006 Subject: [Histonet] mouse labeling with tetracycline Message-ID: <20060331171126.51067.qmail@web31512.mail.mud.yahoo.com> hello: does any one out there have a protocol to use tetracycline to be injected into mice to perform histomorphometry. suresh mathew --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1¢/min. From LRaff <@t> lab.uropartners.com Fri Mar 31 11:34:07 2006 From: LRaff <@t> lab.uropartners.com (Lester Raff) Date: Fri Mar 31 11:34:12 2006 Subject: [Histonet] More on Frozen Section Breast Biopsies Message-ID: <5DA1CA5D0B98A84985B545A24423B822A797@UPLAB01.uplab.local> Laura, Yes, frozen section followed by immediate mastectomy was the standard of care up through at least the early 80's. It worked well on the fairly large, definite masses that were the standard. It also ensured that fresh tissue was available for ER/PR studies, that in those days were actually biochemical assays, not IHC stains. I wrote a paper back then (Raff LJ: Frozen Section Needle Biopsies of the Breast, A Technique of Limited Usefulness. Breast. 8:11-13, 1982) questioning the usefulness of frozen sections on core biopsies. The reasons frozen section of the breast have fallen out of favor are: 1. The popularity of needle biopsies and aspirates in diagnosis of breast lesions 2. The fact that many early, mammographicaly detected, breast lesions lack a definite mass and would be difficult to freeze. 3. Freezing can obscure detail needed for the diagnosis of some subtle lesions. 4. The desirability of allowing the patient to receive the results of their biopsy and have input into the therapeutic decisions. Hope this helps. Say hello to your pathologists for me. Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory ph: 708-486-0076 fax: 708-486-0080 From eshields <@t> bhset.org Fri Mar 31 12:10:17 2006 From: eshields <@t> bhset.org (E Sharon Shields) Date: Fri Mar 31 12:12:08 2006 Subject: [Histonet] TN meeting Message-ID: I need information concerning the TN meeting in June. I gave my mailing away now it looks like I may be able to attend. Thank you Sharon Shields Baptist Hospital of E TN 865 549-4351 Fax 856 632-5316 From Heather.A.Harper <@t> pcola.med.navy.mil Fri Mar 31 12:11:59 2006 From: Heather.A.Harper <@t> pcola.med.navy.mil (Heather.A.Harper@pcola.med.navy.mil) Date: Fri Mar 31 12:14:33 2006 Subject: [Histonet] Need input Message-ID: Hi Everybody, I am the supervisor of a small histology dept. I need input on when or who decides that you need to overhaul the routine you have in place. Is it the pathologists? I have always known a histology dept. to run with certain cut off times. I have a new pathologist, and she wants me to change my routine completely, so that I am doing the bulk of my work at the end of my shift, which is grossing. No matter what I say, she has an answer for every question, has justified it and I feel like what is the point of being a supervisor, if you are not allowed to run the dept. as one should. Our routine was great, no complaints, slides out in timely fashion, never behind but yet I feel why fix something that is not broken? Please give me input on your dept. Cut off times for recuts, special stains, accessioning. Our accepting of specimens cut off time is 0730. Everything gets accessioned. The new pathologist wants the cut off time to be 1100 a.m. I would normally start accessioning for the next day after lunch, 1230. She believes that those specimens should go on that days run. It's confusing but please tell me your routine. Thanks in advance. Heather From Janet.Bonner <@t> FLHOSP.ORG Fri Mar 31 12:56:39 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Mar 31 12:56:54 2006 Subject: [Histonet] Need input References: Message-ID: Our Routine is constantly changing on a weekly basis. ( We just learned we have to rotate on-call for the weekends.) The important thing is to keep the "powers that be" happy, as long as it does not compromise patient care. The supervisor does not necessarily make the rules, but more often enacts them. We are a 24 hour service Laboratory in a Very Big Hospital. Everything is done all the time. I remember feeling like you described just before it dawned on us we needed to go on a first and second shift and then a third shift and then on-call and then.... ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Heather.A.Harper@pcola.med.navy.mil Sent: Fri 3/31/2006 1:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need input Hi Everybody, I am the supervisor of a small histology dept. I need input on when or who decides that you need to overhaul the routine you have in place. Is it the pathologists? I have always known a histology dept. to run with certain cut off times. I have a new pathologist, and she wants me to change my routine completely, so that I am doing the bulk of my work at the end of my shift, which is grossing. No matter what I say, she has an answer for every question, has justified it and I feel like what is the point of being a supervisor, if you are not allowed to run the dept. as one should. Our routine was great, no complaints, slides out in timely fashion, never behind but yet I feel why fix something that is not broken? Please give me input on your dept. Cut off times for recuts, special stains, accessioning. Our accepting of specimens cut off time is 0730. Everything gets accessioned. The new pathologist wants the cut off time to be 1100 a.m. I would normally start accessioning for the next day after lunch, 1230. She believes that those specimens should go on that days run. It's confusing but please tell me your routine. Thanks in advance. Heather _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From binma2 <@t> web.de Fri Mar 31 13:30:36 2006 From: binma2 <@t> web.de (BIN MA) Date: Fri Mar 31 13:30:43 2006 Subject: [Histonet] Immunofluorscence staining on vibrotome sections Message-ID: <1885182490@web.de> hallo everyone, I am doing Immunofluorscence staining on vibrotome sections.My problems are: (1) How to premeablize the tissues? I used 130 um sections from lymph node and embedded in argarose.I tried 0.3% triton or high concentraion of triton x100.But it did not work so well. I also tried with ethanol method. (2) How can I do antigen retrival on these sections? otherwise I can only use polyclonal or some monoclonal antibodies? (3) any suggestion about the incubation time ? Normally I incubated on a shaking table at 4?C overnight. THANK YOU IN ADVANCE Bin Ma MD Molecular Biotechnology German Research Centre of Biotechnology Mascheroder Weg 1 D-38124 Braunschweig Germany Tel: 0049-531-6181293 Fax: 0049-531-6181202 Email: bma@gbf.de binma2@yahoo.com SMS schreiben mit WEB.DE FreeMail - einfach, schnell und kostenguenstig. Jetzt gleich testen! *http://f.web.de/?mc=021192* [http://f.web.de/?mc=021192] From specialstainsqueen <@t> hotmail.com Fri Mar 31 13:36:21 2006 From: specialstainsqueen <@t> hotmail.com (Sherri Anderson) Date: Fri Mar 31 13:36:27 2006 Subject: [Histonet] Fw: Thermo/ Leica In-Reply-To: <1143713391.442bae6fa3529@webmail.shef.ac.uk> Message-ID: Dear Matt: Pauline Fisher from Thermo retired a while ago (almost two years ago, I believe). You could contact Sheila Dandy at sheila.dandy@thermo.com with questions. She is a Product Manager who is based in the U.K. Depending upon what types of questions you have, she may direct your email to a more appropriate person (or persons)....but she would be a good starting point for you and is quite knowledgeable on a wide range of topics/products. Sincerely, Sherri >From: Matt Prideaux >To: MattG >CC: histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] Fw: Thermo/ Leica >Date: Thu, 30 Mar 2006 11:09:51 +0100 > > >The leica rep that we deal with is called Anthony Johnson and he seems >fairly >helpful. His email address is anthony.johnson@leica-microsystems.com > >Matt >-- >Matt Prideaux >Academic Unit of Bone Biology >Division of Clinical Sciences (South) >D Floor (DU20) Medical School >Henry Wellcome Laboratories for Medical Research >University of Sheffield >Beech Hill Road >Sheffield >S10 2RX > >Tel: 0114 271 3783 >Fax: 0114 271 1711 > > > >Quoting MattG : > > > Does anyone have a current email address for Pauline Fisher of Thermo > > (formerly Shandon)? > > > > I'd also be grateful if someone could send me an electronic version of >the > > user manual for the Leica TP1050. > > > > If there are any UK based Leica or Thermo reps on here, could one of you > > please drop me an email as I have a couple of questions I'd like to ask. > > > > Thanks, > > > > Matt Griffiths > > > > > > > > ___________________________________________________________ > > Win a BlackBerry device from O2 with Yahoo!. Enter now. > > http://www.yahoo.co.uk/blackberry > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Express yourself instantly with MSN Messenger! Download today - it's FREE! http://messenger.msn.click-url.com/go/onm00200471ave/direct/01/ From Luis.Chiriboga <@t> med.nyu.edu Fri Mar 31 14:15:26 2006 From: Luis.Chiriboga <@t> med.nyu.edu (Luis Chiriboga) Date: Fri Mar 31 14:11:43 2006 Subject: [Histonet] Region 1 Meeting Registration Reminder Message-ID: Hi Everyone Just a reminder that the 2006 Region 1/NYSHS symposium early registration deadline is April 7th. This years meeting is being held at the Saratoga Springs Holiday Inn, a few minutes walk from beautiful downtown Saratoga Springs, New York. The deadline for reserving a reduced rate hotel room is April 5th. Please visit www.nyhisto.org for a program and registration information. Hope to see you there NYSHS staff From mward <@t> wfubmc.edu Fri Mar 31 14:19:56 2006 From: mward <@t> wfubmc.edu (Martha Ward) Date: Fri Mar 31 14:21:22 2006 Subject: [Histonet] MART-1 on frozen sections Message-ID: <61135F0455D33347B5AAE209B903A304133774D7@EXCHVS2.medctr.ad.wfubmc.edu> I am posting this for a friend who works in a dermatology office lab. Her doctors want to start performing MART-1 on frozen sections. She is having to start from scratch, needing information on kits, equipment needed, etc. Any information I can pass along to her would be greatly appreciated. Thanks in advance for your help. Martha Ward Wake Forest University Baptist Medical Center From Allison_Scott <@t> hchd.tmc.edu Fri Mar 31 14:37:39 2006 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Fri Mar 31 14:37:45 2006 Subject: [Histonet] Lab assistantjob description/competency Message-ID: <1872B4A455B7974391609AD8034C79FC06700C@LBEXCH01.hchd.local> Hello to all in histoland. I hope everyone has a nice weekend . Would someone be willing to share a job description/competnecy for a lab assistant. I am trying to come up with a competency check list for this position. Thanks in advance. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From TJasper <@t> smdc.org Fri Mar 31 15:22:10 2006 From: TJasper <@t> smdc.org (Jasper, Thomas G.) Date: Fri Mar 31 15:21:47 2006 Subject: [Histonet] Need input Message-ID: <1A9F2A6C5762524799A816F1F09744CF1E0A1F@SCREECH.ntcampus.smdc.org> Heather, Tough question(s) to answer. Not knowing the scope of your service and the direction you're heading makes it difficult. Sounds as though your new pathologist has some definite ideas about those topics. I can only tell you about our service, so here it goes... We are a tertiary care facility, just under 400 beds. Our responsibility includes 2 smaller hospitals and multiple clinics in an extensive region. We had approximately 25,000 cases in 2005 and have had steady growth over the last 5 years of 5-10%. We are a 12 hour operation 5AM - 5PM, with pathologist, histologist and autopsy call coverage on weekends. Our pathologists are asked to refrain from calling in requests until 9AM. We will run IHC same day on orders received up until noon (some overnight runs after noon) specials (non-IHC), recuts and deepers up until 2PM. Anything after that is next day unless there is a good "patient care" reason. We accept specimens for accessioning until 4PM. Again, we will make exceptions if there are good "patient care" reasons. That's it in a nutshell. I have no idea what you're facing or what your service is expected to provide. Just off the cuff it sounds like everyone needs to get on the same page for starters. I would think your pathologists should respect your opinion re: what it takes, to get what you're expected to do, done. Good luck, tj -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Heather.A.Harper@pcola.med.navy.mil Sent: Friday, March 31, 2006 12:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Need input Hi Everybody, I am the supervisor of a small histology dept. I need input on when or who decides that you need to overhaul the routine you have in place. Is it the pathologists? I have always known a histology dept. to run with certain cut off times. I have a new pathologist, and she wants me to change my routine completely, so that I am doing the bulk of my work at the end of my shift, which is grossing. No matter what I say, she has an answer for every question, has justified it and I feel like what is the point of being a supervisor, if you are not allowed to run the dept. as one should. Our routine was great, no complaints, slides out in timely fashion, never behind but yet I feel why fix something that is not broken? Please give me input on your dept. Cut off times for recuts, special stains, accessioning. Our accepting of specimens cut off time is 0730. Everything gets accessioned. The new pathologist wants the cut off time to be 1100 a.m. I would normally start accessioning for the next day after lunch, 1230. She believes that those specimens should go on that days run. It's confusing but please tell me your routine. Thanks in advance. Heather _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From Luis.Chiriboga <@t> med.nyu.edu Fri Mar 31 15:37:01 2006 From: Luis.Chiriboga <@t> med.nyu.edu (Luis Chiriboga) Date: Fri Mar 31 15:33:23 2006 Subject: [Histonet] Antibody Recommendation Message-ID: Any recommendations for a cd11b antibody for use against mouse frozen tissue? If you have a reference from the literature even better.... TIA Luis From MVaughan4 <@t> ucok.edu Fri Mar 31 16:23:47 2006 From: MVaughan4 <@t> ucok.edu (MVaughan4@ucok.edu) Date: Fri Mar 31 16:24:17 2006 Subject: [Histonet] Re: marker for mouse vs. human In-Reply-To: Message-ID: James, If you were using immunofluorescence I could tell you that Hoescht staining will demostrate punctate staining in AT-rich regions of the mouse nucleus, whereas human nuclei stain with a smoky appearance. Whether you could use this same distinction using paraffin sections I don't know. Also there are human-specific vimentin (DAKO) and Involucrin (BTI) antibodies available that supposedly will not stain mouse. Mel Melville B. Vaughan, Ph. D. Assistant Professor Department of Biology University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm From Malcolm.McCallum <@t> tamut.edu Fri Mar 31 16:42:17 2006 From: Malcolm.McCallum <@t> tamut.edu (Malcolm McCallum) Date: Fri Mar 31 16:42:24 2006 Subject: [Histonet] advice needed on histo instrumentation Message-ID: Hello everyone, I need some guidance from the wise! I am trying to balance cost with usefulness! I am at a very small college, but we have some $ to purchase some instrumentation. I hope I don't sound clueless, mostly I am looking for advice and sources! I have always done my staining and tissue processing manually. I seldom do anything other than paraffin embedding, so would a tissue processor save me much time, is it worth the cost? which models are best for low volume use? I believe that an automatic stainer might allow me to run the stainer while I teach, manually changing solutions every 30 seconds in monotanous and quickly eats up a day! One study might have 200 to 300 slides on average with an occassional one having more and many having fewer. Basically I am describing reproductive cycles in amphibians and reptiles and doing basic morphology. Some experimental stuff to. What models might work best? Ultracold Freezer: The smallest size I can find is 5 cu.ft. should I go with that or 9 cu. ft? Just used to store some DNA samples and Enzyme samples for classes and limited research. best make model? prices? rotary microtome: I do have a Leica SM2000R sliding microtome, but I would prefer the rotary for most of the stuff I do! Any tips, advice on the best models? I am afraid of the used models! disposable versus permanent knife? Never used disposable, and I do like the permanent blade. Automatic Knife sharpener? I can only find one brand. where is the best place to get Slide warmers and Embedding Ovens? I have a TBS tissue embedding center (TEC-120 or 220 not sure which it is). I have never used one! Always used an embedding oven to embed tissues. What is the advantage? I already have an Olympus BX51 neumarski DIC. Is it possible and for how much to convert to fluorescence? would I need additional lenses? (current lenses: Plan 4x/0.10 [red stripe on it], UPlanF1 10x/0.30 [yellow stripe on it], UPlanF1 20x/0.50 infinity/0.17 [green], UPlanF1 40x/0.75 [blue], UplanF1 100x/1.30 oil infinity/0.17 [white]). I used a little bit of fluorescence during graduate studies and would like to develop my skills if it is possible! I also have an Olympus DP11 Camera system attached to the numarski. Would anyone be able to recommend an economical computer imaging system? This camera was here when I got here, we always used a computer uplink and using microcards is pretty inconvenient! I don't have any instructions for it, I know how to operate it, but I don't even know if it can be linked directly to a PC. This stuff was all left by the former faculty member and I have to admit there are probably some of you out there saying "I landed in heaven!" Anyway, any help in this regard would be great. I would really like to eventually develop a histotechniques course, and most of what I need is Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-233-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html From gibi55 <@t> yahoo.com Fri Mar 31 20:00:08 2006 From: gibi55 <@t> yahoo.com (Gino Bianchi) Date: Fri Mar 31 20:00:15 2006 Subject: [Histonet] looking for a tissue processor Message-ID: <20060401020008.22352.qmail@web30314.mail.mud.yahoo.com> Hi, I`m looking for a Citadel or Technicon tissue processor in working order. --------------------------------- Yahoo! Messenger with Voice. PC-to-Phone calls for ridiculously low rates. From gibi55 <@t> yahoo.com Fri Mar 31 20:02:47 2006 From: gibi55 <@t> yahoo.com (Gino Bianchi) Date: Fri Mar 31 20:02:51 2006 Subject: [Histonet] looking for a tissue processor Message-ID: <20060401020248.23127.qmail@web30314.mail.mud.yahoo.com> Hi, I?m looking for a Citadel or Technicon tissue processor in good shape. Dr. Gino I Bianchi gibi55@yahoo.com --------------------------------- New Yahoo! Messenger with Voice. Call regular phones from your PC and save big. From marjoh3 <@t> telus.net Fri Mar 31 20:50:33 2006 From: marjoh3 <@t> telus.net (Marilyn Johnson) Date: Fri Mar 31 20:50:36 2006 Subject: [Histonet] Tissue Tek II Embedding Center 4603 Message-ID: <001e01c65537$0ba2f8f0$6501a8c0@VALUED20606295> Hi Histonetters, I have an old Tissue Tek II embedding center, model 4603, that requires a heating element for the front working platform to place cassettes before embedding. I know this machine is obsolete, but are there still any parts available? Any replies would be greatly appreciated. Thank you in advance. Marilyn Johnson