From L.Driessen <@t> orthop.umcn.nl Thu Jun 1 00:37:21 2006 From: L.Driessen <@t> orthop.umcn.nl (L.Driessen@orthop.umcn.nl) Date: Thu Jun 1 00:37:26 2006 Subject: [Histonet] RE: Extra hard PMMA? Message-ID: <5169A2936294864EACC9DD433D003E7408D484@umcnet30.umcn.nl> Hello Paul, I've done some testing on this, together with mechanical loading. The hardest plastic contained 90 ml MMA, 10 ml Dibutylphtalate and 1 gr ?,? Azobutyronitril. Mechanical testing with a preload of 10N and a mainload of 40N gave an dentation of 0,04 mm. Further reduction in softener didn't lead to harder plastic. Reduction of hardener also didn't have any extra effect except for much shrinking. L?on Driessen Orthopaedisch Research Lab UMC St. Radboudziekenhuis, Nijmegen 024-3615145/3614932 l.driessen@orthop.umcn.nl Date: Wed, 31 May 2006 12:15:03 -0400 From: "Monfils, Paul" Subject: [Histonet] Extra hard PMMA? To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <09C945920A6B654199F7A58A1D7D1FDE0171771F@lsexch.lsmaster.lifespan.org> Content-Type: text/plain; charset="iso-8859-1" Does anyone have experience in using harder than usual PMMA for sectioning extremely hard specimens? What kind of softener, and how much, do you add to the methacrylate? Is it possible to section pure PMMA without any softeners added? (using a powerful motorized sliding microtome and tungsten carbide knives) From kemlo.rogerson <@t> waht.swest.nhs.uk Thu Jun 1 01:57:30 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Jun 1 01:57:07 2006 Subject: [Histonet] NOW Alcohol / Xylene storage in flammable storagec abinet Message-ID: Alcohol floats unless physically made to mix with water, xylene floats even if mixed; sprinklers appear to be an efficient system in disseminating the fire all over the place. Chip Fat fires in the UK must emulate flaming paraffin wax and we incinerate loads of betas by them getting drunk, putting on the chip pan, it igniting and them waking up in a drunken stupor and throwing water at it. In an attempt to save this valuable commodity we ask them to put a dampened cloth over it after turning of the flames; wonder where they get that from. Their trousers? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 The greatest mistake in the treatment of diseases is that there are physicians for the body and physicians for the soul, although the two cannot be separated. --Plato This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From carl.hobbs <@t> kcl.ac.uk Thu Jun 1 02:14:15 2006 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Thu Jun 1 02:14:36 2006 Subject: [Histonet] re:Abs against phosphoproteins Message-ID: <001301c6854a$fdf4f0c0$112b5c9f@Carlos> I agree with Andrea. Sure, fixation ( and don't forget pwax processing) may well destroy/mask your protein irreversibly. Such that no amount of Ag retrieval will work. Always is a "luck of the draw" situation. Also, if the Ab is raised against a peptide, there is no guarantee that it will recognise the native protein, either. The only way to confirm that your pwax- negative staining is "real", would be to use T/C cells that have been, for eg, irradiated, then fixed and stained for your p-Histone. Then, you would make a pellet of another plate of irradiated- fixed cells and process that to pwax. If the Ab is negative on these cells, whatever pretreatment you do, then it ain't gonna work on pwax sections, full stop. If you must use archival material, then try to find another Ab, raised for the same protein. I'm sure others will make more positive comments/ give alternative suggestions. Try asking here too.... http://www.immunoportal.com/index.php Best wishes Carl From AnthonyH <@t> chw.edu.au Thu Jun 1 02:17:14 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Jun 1 02:17:28 2006 Subject: [Histonet] NOW Alcohol / Xylene storage in flammable storagecabinet Message-ID: Ah Kemlo, You always make me ponder and imagine the situation... And then I laugh - well done Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: Kemlo Rogerson [mailto:kemlo.rogerson@waht.swest.nhs.uk] Sent: Thursday, 1 June 2006 4:58 PM To: Tony Henwood; Rene J Buesa; Deltour, Douglas D. (HM2); bamoe@gundluth.org; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] NOW Alcohol / Xylene storage in flammable storagecabinet Alcohol floats unless physically made to mix with water, xylene floats even if mixed; sprinklers appear to be an efficient system in disseminating the fire all over the place. Chip Fat fires in the UK must emulate flaming paraffin wax and we incinerate loads of betas by them getting drunk, putting on the chip pan, it igniting and them waking up in a drunken stupor and throwing water at it. In an attempt to save this valuable commodity we ask them to put a dampened cloth over it after turning of the flames; wonder where they get that from. Their trousers? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 The greatest mistake in the treatment of diseases is that there are physicians for the body and physicians for the soul, although the two cannot be separated. --Plato This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From livieira <@t> ualg.pt Thu Jun 1 05:51:44 2006 From: livieira <@t> ualg.pt (Lina Vieira) Date: Thu Jun 1 05:54:11 2006 Subject: [Histonet] mitosis and meiosis Message-ID: <00b201c68569$5f6bd300$2914100a@labhistologia> I wanted to thank all who had take your advice about the methods for microscopic demonstration of mitosis and meiosis. I forget to explain that I need definitive preparations! An I don?t have mammalians tissues disponibles, only fish and plant tissues! Best Regards Lina Lina Vieira Universidade do Algarve ITUCCA Lab Histologia Campus de Gambelas 8000-117 Faro Portugal From DELONG_CYNTHIA_A <@t> LILLY.COM Thu Jun 1 07:02:11 2006 From: DELONG_CYNTHIA_A <@t> LILLY.COM (Cynthia A Delong) Date: Thu Jun 1 07:02:18 2006 Subject: [Histonet] Attention: Joe Galbraith - Tau AT8 Message-ID: This message is to Joe Galbraith- I was searching the Histonet archives and I ran across his protocol for Tau AT8 from endogen. It said "1:2000 dil, no pretreatment, 15-15 dakoautostainer" I was needing more details. I have been working with the biotinylated MN1020B and also I tried the MN1020. Joe or anyone else who has gotten this antibody to work in paraffin 8 um sections please send me the protocol. I tried the biotinylated in HRP. Antigen retrieval in both citrate and formic acid 3% Hydrogen Peroxide in PBS 5 min, Power Block 5 min dilutions ranging from 100-2000, for 2 hrs and ON Vector ABC 30 min - 1 hr, DAB 5- 10 min. I just received the MN1020 and tried it no pretreament, 3% Hydrogen peroxide 5 min, Power Block 5 min, 1:2000 antibody 15 minutes, 1:500- Southern Biotech goat anti mouse kappa FITC- 30 min. Thank you Cindy Cynthia A DeLong LRL - Corporate Center DC0533 - 48B/3042 355 E Merrill St Indianapolis, IN 46225 phone: 317-276-7635 fax: 317-277-6146 From mauger <@t> email.chop.edu Thu Jun 1 07:21:33 2006 From: mauger <@t> email.chop.edu (Joanne Mauger) Date: Thu Jun 1 07:22:12 2006 Subject: [Histonet] antibodies against phosphorylated proteins Message-ID: Heike, We use H2A.X on human FFPE tissue. The dilution is 1:50 and HIER with citrate buffer, pH 6. We use ABC detection. Works well. Jo Mauger >>> "Heike Grabsch" 05/31/06 10:32 AM >>> I am working with formalin fixed paraffin embedded tissue and trying to detect phosphorylated proteins by immunohistochemistry. The antibodies are meant to be specific for the phosphorylated protein only. The one antibody I am trying to work up in particular is the phospho-H2AX from Upstate. Is there anybody out there who has an idea whether and if then how fixation may effect phosphorylation sides? Can they be lost or destroyed due to fixation? So the staining could be false negative? I hope somebody can comment on this Thanks, Heike Dr Heike Grabsch, MRCPath MD Gastrointestinal Research Group Pathology and Tumour Biology JIF Building, Level 4, Room 4.13 Leeds Institute for Molecular Medicine St James's University Hospital Beckett Street Leeds LS9 7TF United Kingdom phone 0044 113 3438626 fax 0044 113 3438431 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lcastillos <@t> icoria.com Thu Jun 1 07:40:30 2006 From: lcastillos <@t> icoria.com (Castillos, Luminita) Date: Thu Jun 1 07:40:37 2006 Subject: [Histonet] NK markers for human endometrial tissue Message-ID: > Hello, > Are there any NK markers for endometrial tissue that work using > immunohistochemistry procedures on FFPE human tissues? Also, I would > like to ask if somebody can share with me some H&E pictures of ectopic > versus eutopic endometrium and what is the real definition of these > two types of endometrium?. > Thanks in advance, > Luminita, > > > > Luminita Castillos, Ph.D. > Research Scientist > Cogenics(r), A Division of Clinical Data(r) > Comprehensive Pharmacogenomics & Molecular Services(tm) > 108 Alexader Dr. > RTP, NC 27709 > Tel: 919-425-2967 > www.cogenics.com > > > > From lao_ji <@t> yahoo.com Thu Jun 1 08:56:12 2006 From: lao_ji <@t> yahoo.com (Jimmy Lao) Date: Thu Jun 1 08:56:18 2006 Subject: [Histonet] Re: Cre, eGFP, and B-Gal Antibodies In-Reply-To: Message-ID: <20060601135612.88278.qmail@web30709.mail.mud.yahoo.com> Rhonda, I feel the same way to Teri Johnson, Here is my working lab protocol website, at least B-gal staining there, we used Biogenesis goat-anti B gal, in some tissue work but backgound still here. I also want to hnow what others comments. Good Luck! Jimmy http://saturn.med.nyu.edu/research/dg/joynerlab/protocols.html --- "Johnson, Teri" wrote: > Hi Rhonda, > > I wish you nothing but luck with these! Out of the > three, I'd say you will probably have success with > the eGFP - it has been used successfully quite a bit > in publications. We use Novus Biologicals rabbit > polyclonal eGFP (new purified antibody) and we > really like the results we're getting so far. > > Regarding Cre and B-gal, we have had no luck with > either of them. The cre antibodies have not worked > at all (but I think should work well on cultured > cells), and the B-gal has inconsistent staining that > I can't mirror with x-gal staining. Between the > histology facility and the researchers at our > institute, I think we've tried practically every Cre > and B-gal commercially available and we're not > getting any usable results. > > Let me know if you get some off list replies, I'm > all ears (eyes!). > > Best wishes, > > Teri Johnson > Stowers Institute for Medical Research > Kansas City, MO 64110 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From kappeler <@t> patho.unibe.ch Thu Jun 1 08:55:36 2006 From: kappeler <@t> patho.unibe.ch (Andi Kappeler) Date: Thu Jun 1 08:56:20 2006 Subject: [Histonet] IHC for pancreatic trypsin Message-ID: <023e01c68583$2114d060$27955c82@patho.unibe.ch> I am looking for an antibody against pancreatic trypsin that will work on FFPE tissue (--> acinar cell carcinoma, pancreas). Any suggestions? A paper by Ohike et al. (Virchows Arch 2004; 445:231) indicates a monoclonal (clone??) from Ventrex Labs, Portland OR, however I have difficulties in locating Ventrex on the web (or otherwise). Does anybody have contact information for Ventrex? Thanks a lot! Andi Kappeler Institute of Pathology, University of Bern, Switzerland From yichaowu <@t> hotmail.com Thu Jun 1 09:29:56 2006 From: yichaowu <@t> hotmail.com (yichao wu) Date: Thu Jun 1 09:30:03 2006 Subject: [Histonet] SIGN OFF HISTONET Message-ID: Sincerely yours, Yichao WU Email: yichaowu@hotmail.com or wuyc_nju@yahoo.com From kappeler <@t> patho.unibe.ch Thu Jun 1 10:28:06 2006 From: kappeler <@t> patho.unibe.ch (Andi Kappeler) Date: Thu Jun 1 10:28:53 2006 Subject: [Histonet] IHC for pancreatic trypsin References: <355C35514FEAC9458F75947F5270974D67CEC3@usctmx1103.merck.com> Message-ID: <026401c68590$0d38aa00$27955c82@patho.unibe.ch> Thanks Brett! ... and as usual: never trust the Materials & Methods section of a paper: Of cause they had put Portland OR there. It is my impression anyway, that in many papers the person who wrote the M&M section does not even know where the lab is... Regards, Andi ----- Original Message ----- From: "Connolly, Brett M" To: "'Andi Kappeler'" Sent: Thursday, June 01, 2006 5:02 PM Subject: RE: [Histonet] IHC for pancreatic trypsin > Andi, > > I did a little searching, they are/were at 217 Read Street, Portland, > Maine > 04104 (not Portland, Oregon) and are a subsidiary of Hycor Biomedical. > > Maybe if you can find contact info for Hycor they can help you. > > Regards, > Brett > > Brett M. Connolly, Ph.D. > Merck & Co., Inc. > MRL, Imaging Research > WP-44K > PO Box 4 > West Point, PA 19486 > PH 215-652-2501 > fax. 215-993-6803 > e-mail. brett_connolly@merck.com > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andi > Kappeler > Sent: Thursday, June 01, 2006 9:56 AM > To: Histonet > Subject: [Histonet] IHC for pancreatic trypsin > > > I am looking for an antibody against pancreatic trypsin that will work on > FFPE tissue (--> acinar cell carcinoma, pancreas). Any suggestions? A > paper > by Ohike et al. (Virchows Arch 2004; 445:231) indicates a monoclonal > (clone??) from Ventrex Labs, Portland OR, however I have difficulties in > locating Ventrex on the web (or otherwise). Does anybody have contact > information for Ventrex? Thanks a lot! > > Andi Kappeler > Institute of Pathology, University of Bern, Switzerland > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------------------------------------------------------ > Notice: This e-mail message, together with any attachments, contains > information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New > Jersey, USA 08889), and/or its affiliates (which may be known outside the > United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as > Banyu) that may be confidential, proprietary copyrighted and/or legally > privileged. It is intended solely for the use of the individual or entity > named on this message. If you are not the intended recipient, and have > received this message in error, please notify us immediately by reply > e-mail and then delete it from your system. > ------------------------------------------------------------------------------ > From H.I.Grabsch <@t> leeds.ac.uk Thu Jun 1 10:51:07 2006 From: H.I.Grabsch <@t> leeds.ac.uk (Heike Grabsch) Date: Thu Jun 1 10:51:39 2006 Subject: [Histonet] antibodies against phosphorylated proteins Message-ID: Jo, What do you use as positive control tissue, please? Heike > -----Original Message----- > From: Joanne Mauger [mailto:mauger@email.chop.edu] > Sent: 01 June 2006 13:22 > To: Heike Grabsch; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] antibodies against phosphorylated proteins > > Heike, > > We use H2A.X on human FFPE tissue. The dilution is 1:50 and HIER with > citrate buffer, pH 6. We use ABC detection. Works well. > > Jo Mauger > > >>> "Heike Grabsch" 05/31/06 10:32 AM >>> > I am working with formalin fixed paraffin embedded tissue and trying > to > detect phosphorylated proteins by immunohistochemistry. The antibodies > are meant to be specific for the phosphorylated protein only. > The one antibody I am trying to work up in particular is the > phospho-H2AX from Upstate. > Is there anybody out there who has an idea whether and if then how > fixation may effect phosphorylation sides? Can they be lost or > destroyed > due to fixation? So the staining could be false negative? > I hope somebody can comment on this > > Thanks, > Heike > > Dr Heike Grabsch, MRCPath MD > Gastrointestinal Research Group > Pathology and Tumour Biology > JIF Building, Level 4, Room 4.13 > Leeds Institute for Molecular Medicine > St James's University Hospital > Beckett Street > Leeds > LS9 7TF > United Kingdom > > phone 0044 113 3438626 > fax 0044 113 3438431 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Reuel.Cornelia <@t> tsrh.org Thu Jun 1 11:33:01 2006 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Thu Jun 1 11:35:03 2006 Subject: [Histonet] Inflammatory markers and Apoptotic markers Message-ID: Hello, I just wanted to know what inflammatory cells markers for Formalin fixed tissue for IHC. I have CD8,CD4,CD 20,LCA,CD 68.Is there any additional markers that you routinely do for inflammatory cells. The other thing, Can anyone suggest a marker for apoptosis base on acute inflammatory response? I hope my question make sense, I am asked to do IHC in formalin fixed tissue on inflammatory cells on a research project. Thank you. Reuel Cornelia TSRH Dallas, TX 214-559-7766 ******************************************************************************************************************* Texas Scottish Rite Hospital for Children is one of the nation's leading pediatric centers for the treatment of orthopedic conditions and learning disorders, like dyslexia. This email transmission and/or its attachments may contain confidential health information, intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing it to any other party unless required to do so by law and is required to delete/destroy the information after its stated need has been fulfilled. If you are not the intended recipient, any disclosure, copying, distribution or action taken in reliance on the contents of this email transmission is prohibited. If you have received this information in error, please notify the sender immediately and delete this information. We appreciate your efforts to protect the children's confidential information. ******************************************************************************************************************* From mauger <@t> email.chop.edu Thu Jun 1 11:47:33 2006 From: mauger <@t> email.chop.edu (Joanne Mauger) Date: Thu Jun 1 11:48:06 2006 Subject: [Histonet] antibodies against phosphorylated proteins Message-ID: Heike- We use tonsil. Jo >>> "Heike Grabsch" 06/01/06 11:51 AM >>> Jo, What do you use as positive control tissue, please? Heike > -----Original Message----- > From: Joanne Mauger [mailto:mauger@email.chop.edu] > Sent: 01 June 2006 13:22 > To: Heike Grabsch; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] antibodies against phosphorylated proteins > > Heike, > > We use H2A.X on human FFPE tissue. The dilution is 1:50 and HIER with > citrate buffer, pH 6. We use ABC detection. Works well. > > Jo Mauger > > >>> "Heike Grabsch" 05/31/06 10:32 AM >>> > I am working with formalin fixed paraffin embedded tissue and trying > to > detect phosphorylated proteins by immunohistochemistry. The antibodies > are meant to be specific for the phosphorylated protein only. > The one antibody I am trying to work up in particular is the > phospho-H2AX from Upstate. > Is there anybody out there who has an idea whether and if then how > fixation may effect phosphorylation sides? Can they be lost or > destroyed > due to fixation? So the staining could be false negative? > I hope somebody can comment on this > > Thanks, > Heike > > Dr Heike Grabsch, MRCPath MD > Gastrointestinal Research Group > Pathology and Tumour Biology > JIF Building, Level 4, Room 4.13 > Leeds Institute for Molecular Medicine > St James's University Hospital > Beckett Street > Leeds > LS9 7TF > United Kingdom > > phone 0044 113 3438626 > fax 0044 113 3438431 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Graham.Boorse <@t> asu.edu Thu Jun 1 12:25:56 2006 From: Graham.Boorse <@t> asu.edu (Graham Boorse) Date: Thu Jun 1 12:26:03 2006 Subject: [Histonet] Microm HM 500M and HM 310 Instruction manual Message-ID: <099A0F91F8205A44B7C9E8C17A33C85812047C@westex3.west.asu.edu> Hello Experts, I am a new faculty member at Arizona State University that has inherited a Microm HM 500M cryostat and a Microm HM 310 rotary microtome as part of my startup package. Unfortunately instruction manuals were not included. I am trying to find someone who may have the same pieces of equipment including the instruction manuals that could provide me a copy through the mail. I, of course, would be willing to compensate for copy services, postage, and time spent on this project. Thanks Graham Boorse ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Graham C. Boorse, Ph.D. Assistant Professor Department of Integrated Natural Sciences Arizona State University at the West Campus Mail Code 2352 PO Box 37100, Phoenix, AZ 85069 Phone (602)543-6936; Fax (602)543-6073 Graham.Boorse@asu.edu ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ From TJasper <@t> smdc.org Thu Jun 1 12:54:21 2006 From: TJasper <@t> smdc.org (Jasper, Thomas G.) Date: Thu Jun 1 12:54:40 2006 Subject: FW: [Histonet] Ventana Benchmark Message-ID: <1A9F2A6C5762524799A816F1F09744CF1E0ABC@SCREECH.ntcampus.smdc.org> -----Original Message----- From: Jasper, Thomas G. Sent: Thursday, June 01, 2006 11:47 AM To: 'Sanders, Julie, VHACIN' Cc: 'histonet-bounces@lists.utsouthwestern.edu' Subject: RE: [Histonet] Ventana Benchmark Hello all, I've been following this thread with some interest and must say there are good points all around. It just goes to show that life is not black and white, a lot of grey areas here. I'm certain we all agree we wish things weren't so costly. I guess what I try and do is look at "real cost" that is, for us (clinical) within our scope of service. Someone made a very good point about research vs. clinical and the time constraints etc. I certainly agree that the research world (diverse as it is) for the most part can probably afford(real cost now)to spend time that clinical services don't have. I have worked in both worlds. Our service has done the...Ventana - Dako - Dako/Ventana - Ventana dance with automated IHC which I suppose validates another point someone made about customer loyalty etc. We currently run 3 Ventana Benchmark XTs and 2 NexES. Do I think the costs are high? Sure. Again what is it worth to you? I'm not for gouging patients with lab testing bills, but seriously, at least in the good ol' USA, you can look at many other factors which have led to skyrocketing health care costs. Number one on my HIT PARADE are the insurance companies. Don't be fooled folks, these organizations are the bane of health care. My wife worked for many years as a patient account rep in a clinical setting. Reimbursement is an unbelievably convoluted game. And like most high stakes games, the House has the odds in their favor. But, I digress. What we are looking for is consistency, speed, reproducibility and reliability. I also like to believe that part of the cost to my institution goes to well educated people working in technical support roles etc. Good technical support is extremely valuable. I am very much in favor of having well educated staff that understand the science behind the technology. We do everything within our power to maintain that here. Again, I realize this may not be something everyone, everywhere can do. So for those of you reading this...what's my point? Well, I'm willing to pay what I have to, to run Ventana...at least right now. And don't get me wrong I think Dako is a good company, new computer system and all. Both have had their ups and downs. Also, to the person whose boss is asking all the time about why the high costs and how to reduce them. I would suggest looking outside of Histology. Comparitively speaking within Laboratory Services, Histology equipment, reagents, staff, etc. are not overly costly. I sit in meetings and listen to what other departments need to budget for things and my jaw just drops. And to add even more perspective, I had this fact pointed out to me last month during National Laboratory Week...4 cents out of every US healthcare dollar spent goes to lab services, over 80% of patient care decisions made are based on lab testing. That's a helluva deal! Makes Histology spending seem pretty tame doesn't it. So do what you gotta do as cost effectively as possible. One size does not fit all. Thanks for the soapbox! Thomas Jasper HT (ASCP) BAS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Sanders, Julie, VHACIN Sent: Tuesday, May 30, 2006 6:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana Benchmark I agree with Joe, the cost of running the Ventana is high. My chief is always trying to figure out a way for us to not do immunos in house just because of this. With that said, our Ventana sales rep has given us the best prices possible for the volume we run, which is not that high compared to large hospitals/labs. I wish there were some way to keep the cost down for running this particular machine (we have a Benchmark XT), but there isn't and costs can only go up, as they do every year. However, we like Ventana, the technology, tech support, and sales support. Our pathologists would walk if we ever stopped using it as it has provided them with the best, most reliable technology we have found on the market. "How come every one talks about the reliability and walk away technology, but no one ever talks about the price of running these machines? Shouldn't cost be a factor also? I've been in budget meetings all week. The only thing I heard was how expensive it is to run immunos." Julie Sanders, BA, HT(ASCP) Supervisor, Anatomic Pathology Cincinnati VAMC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From detmar <@t> mshri.on.ca Thu Jun 1 13:29:42 2006 From: detmar <@t> mshri.on.ca (Jacqui Detmar) Date: Thu Jun 1 13:29:52 2006 Subject: [Histonet] caspase-8 antibody for IHC Message-ID: Hi all. I have been looking for a mouse-specific anti-caspase-8 antibody that is good for IHC. I can find a lot of antibodies against caspase-8 for Western blotting, but so far, only 2 for IHC (that are mouse-specific!). The two that I have narrowed down are from ABR (PA1-21140) and Abcam (ab4052). I was wondering if anyone has any experience with these antibodies and/or can recommend an antibody from another source. The sections I will be probing with the antibody are murine placental and ovarian tissue. Thanks a bunch, Jacqui Detmar, Ph.D. candidate Samuel Lunenfeld Research Institute, Mount Sinai Hospital Toronto, Ontario, Canada From carl.hobbs <@t> kcl.ac.uk Thu Jun 1 14:14:19 2006 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Thu Jun 1 14:15:08 2006 Subject: [Histonet] re: GAD vs. paraformaldehyde Message-ID: Without going into the debate, may I offer two example pics of Chemicon's GAD 65/67 Ab for you to look at? Here: http://www.immunoportal.com/index.php (in the Immunohisto picture gallery) It seems, to me, to work very well in pwax sections of rat brain, after HIER; brain was immersion - fixed in 4% Formalin in PBS pH7.3( made using the commercially available 37% Formalin solution). It works equally well in PFA-derived fixing fluid, made to the same recipe, whether immersion -fixed or perfusion -fixed, IMHO. Best wishes Carl From carolb <@t> phys.mcw.edu Thu Jun 1 14:31:22 2006 From: carolb <@t> phys.mcw.edu (Bobrowitz, Carol) Date: Thu Jun 1 14:31:29 2006 Subject: [Histonet] att: Bryan D. Llewellyn Message-ID: <8F78639AC56F4143B267FE5F5A1B92C80E4301@guyton.phys.mcw.edu> Hello Bryan, My co-worker just brought in a copy of a special stain, MSB for Fibrin, copied from the StainsFile site. As I was gathering information I noticed when I clicked on Martius Yellow CI# 10315 its shows up as Fast Yellow CI#13015. I was wondering which ??? Yellow is required to do the MSB stain. Fast Yellow, CI# 13015, is used in the Wallart & Honette's Trichrome stain. Your help will be appreciated Thank you, Carol Ann Bobrowitz Department of Physiology - Histology Medical College of Wisconsin cbobrowi@mcw.edu From sae2001 <@t> med.cornell.edu Thu Jun 1 14:52:06 2006 From: sae2001 <@t> med.cornell.edu (Scott A. Ely) Date: Thu Jun 1 14:52:17 2006 Subject: [Histonet] IP stainers Message-ID: <3f50d238218ae.447f0d26@med.cornell.edu> Andrea, We have many of the Vision Biosystems (Bond Max) machines and thery are excellent. Scott Ely, MD MPH Section of Hematopathology Department of Pathology Weill Medical College of Cornell University New York Presbyterian Hospital 525 E. 68th Street New York, NY 10021 PH: 212-746-2442 Legal Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the original intended recipient(s) selected by Dr. Ely and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the recipient specified by Dr. Ely, please contact the sender by reply e-mail and destroy all copies of the original message. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea C. Bilger Sent: Wednesday, 31 May 2006 11:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ventana vs Dako I ave been reading with interest the mailings about the 2 top IP stainers. We have 2 Ventana XT's in our lab. Have found them very expensive to operate as everyone else has. The Pathologists wanted us to continue with Ventana due to only having one knowlegable IP tech and wanting consistancy. You have to have some knowlege of IP's even to operate the Ventana machines. Some reps make it sound goof proof but it isn't. We have had trouble with consistancy in a couple of detection kits. One was stronger and all our stains were too hot. Adjusted the stains and the next kit was back to the weaker strength. Had to adjust all the stains back again. What a pain in the ...... Has anyone had any experience with another IP stainer from Vision Biosystems? I looked at one at a National Histo convention and it sounded like a cross between the Dako and the Ventana. I think it was more of an open system but used bar codes on the slides. Antigen retrieval was done on the stainer. Reagents were supposed to be cheaper and not proprietary. It had a feature where you could start a stat stain after the run was started. I would be interested to hear anyones experience with this machine. Andrea From jtrynak <@t> hotmail.com Thu Jun 1 16:03:24 2006 From: jtrynak <@t> hotmail.com (John Rynak) Date: Thu Jun 1 16:03:32 2006 Subject: [Histonet] Wanted: Histology Instrumentation Sales Professionals - Various Regions Message-ID: Regional Account Representative - Clinical Instrumentation / Consumables Experiencing tremendous growth within their North American operations. This growth is due to the recent launch of their FDA approved automated histology instrumentation system, and the growing antibody and reagent consumable product line for the histology market. Due to the new launch of these products they are currently looking for Senior Account Representatives, within the following regions: Pacific Northwest Coast (CA-WA), Upper Midwest (MN, WI, ND,SD), Chicago Metro, Southeast (VA, NC, SC) Southcentral (TEXAS), Metro NYC, and New England. The ideal candidate will have had 3+ years of experience selling capital equipment and associated consumables into a clinical diagnostics laboratory. This person will have had prior experience selling to end user or laboratory manager ideally within the histopathology sector, and a BS/MS in a life science discipline. This position requires the ability to travel 75% of the time in a multi-state region. If you have any further questions feel free to contact me at 617-661-1067 or via email at jtrynak@scigenium.com. If you know of someone who may be interested in this opening, please have him or her forward a Word copy of his or her resume to me. Thank you very much for taking the time. I look forward to hearing from you soon. Best regards, John Rynak SciGenium 617-661-1067 [1]jtrynak@scigenium.com References 1. mailto:jtrynak@scigenium.com From JWEEMS <@t> sjha.org Thu Jun 1 18:46:53 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Thu Jun 1 18:46:18 2006 Subject: [Histonet] Red gram stain Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202736CE7@sjhaexc02.sjha.org> No answers? j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Weems, Joyce Sent: Friday, May 26, 2006 1:09 PM To: Histonet Subject: [Histonet] Red gram stain Hello All, One of our pathologists has just seen a reference to a "Red Gram Stain" for histoplasmosis in the Color Atlas of Surgical Pathology by Gutherie and Fawkes, pg 98, frame 298. Is anyone familiar with this stain? Thanks, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From pathrm35 <@t> adelphia.net Thu Jun 1 19:29:46 2006 From: pathrm35 <@t> adelphia.net (Ron Martin) Date: Thu Jun 1 19:29:58 2006 Subject: [Histonet] histotechs grossing and CLIA regs (again) Message-ID: <000a01c685db$a85802b0$27233418@Pathrm35> Fellow techs, I know this topic has been mentioned several times in the past but here I go again. I think the general interpretation ( from previous postings) on CLIA regs for histotechs grossing is the following: 1) After 1995 a person must have a minmum of a AS degree in a science ( Bio, Chem, Biochem, MLT, etc). 2) Before 1995 no degree but documented training in grossing. My questions are: Do these CLIA regs apply to all independent labs including POL's (Physician Office Labs)? I am currently with a POL derm lab and this question has come up. Also, in the state of Florida, is a state of Florida tech license required to gross ( in my case all derms)? Sorry to rehash old topics but I just need some additional input. Thanks, Ron Martin From a.tuck <@t> uq.edu.au Fri Jun 2 02:19:27 2006 From: a.tuck <@t> uq.edu.au (Mr Andrew Tuck) Date: Fri Jun 2 02:19:38 2006 Subject: [Histonet] Whole brain perfusion of mice Message-ID: Hi I was looking to use micro CT (computer tomography X ray) to examine newborn (P0) mice brains. The area we are most interested in is measuring the ventricle size in these animals. However we also want to look at the size and shape of other regions such as fiber tracts, etc..In the past this was done by doing 50um slices, putting these on slides, photographing the slices and using software to calculate the ventricle size. Using micro CT offers the opportunity to image a whole intact brain. However, in the first scan we performed of a fixed mouse brain you could see the shaodw outline of the brain but no internal detail. Osmium tetroxide is the heavy metal of choice to produce good X ray contrast, but does anyone know how you can perfuse an entire intact brain with it ? I have not been able to find any protocols, or previous articles which have done this. Also I have read that osmium does not penetrate whole tissue very well. If this is the case, is there another fixative that would give good X ray contrast and penetrate the tissue well? Thanks Andrew Tuck Research Assistant Queensland Centre for Schizophrenia Research School of Biomedical Sciences University of Queensland Australia Tel: 07 3346 2368 From Olivier.Leroux <@t> UGent.be Fri Jun 2 03:31:44 2006 From: Olivier.Leroux <@t> UGent.be (Olivier Leroux) Date: Fri Jun 2 03:31:55 2006 Subject: [Histonet] section adhesion on slides Message-ID: <1149237104.447ff770acb17@mail.ugent.be> Hello, Currently we are using vectabond coating to treat our slides for a good adhesion of technovit 7100 sections. Does anybody know a cheaper alternative? regards, Olivier -- Olivier Leroux Ghent University Department of Biology - Pteridological Section K.L. Ledeganckstraat 35 B-9000 Belgium http://www.pteridology.ugent.be Olivier.Leroux@UGent.be From pex0220 <@t> yahoo.com.cn Fri Jun 2 06:08:07 2006 From: pex0220 <@t> yahoo.com.cn (pex) Date: Fri Jun 2 06:08:23 2006 Subject: [Histonet] Microwave for antigen retrieval Message-ID: <20060602110807.41455.qmail@web15207.mail.cnb.yahoo.com> Hello, I have got several leg paraffin sections from newborn mice to do immunofluorescence. Now I would like to know if I can use microwave method for antigen retrieval and it will destroy the cartilage. Thank you. Guofeng __________________________________________________ ¸Ï¿ì×¢²áÑÅ»¢³¬´óÈÝÁ¿Ãâ·ÑÓÊÏä? http://cn.mail.yahoo.comFrom akbitting <@t> geisinger.edu Fri Jun 2 07:20:12 2006 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Jun 2 07:20:54 2006 Subject: [Histonet] Sakura coverslipper Model 6400 Message-ID: Hi Histonetters, I'm demoing this automated glass coverslipper for two weeks. The person who currently repairs (frequently) my tape coverslippers said "if I think I have problems now, moving to a glass coverslipper will be even worse". What are your opinions? Thanks, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From DDDeltour <@t> mar.med.navy.mil Fri Jun 2 07:44:17 2006 From: DDDeltour <@t> mar.med.navy.mil (Deltour, Douglas D. (HM2)) Date: Fri Jun 2 07:44:34 2006 Subject: [Histonet] Sakura coverslipper Model 6400 Message-ID: <3F500F8B416C554EBB21FF16642F72E959CEB1@marxchg03.mar.med.navy.mil> I will NEVER go back to glass. Our Sakura tissue-tek SCA coverslippers are workhorses. They have not needed service in over three years. The previous supervisor ordered a Tissue-Tek Glass coverslipper and it is collecting dust. It is not as fast as the tape and the techs feel the need to watch it as it coverslipps because they do not trust it. There are many myths and reasons that people do not like tape coverslippers. I will let the others get into those reasons but for my ulcer I would not have anything else but the tape. Good luck! Douglas Deltour HT(ASCP) -----Original Message----- From: Angela Bitting [mailto:akbitting@geisinger.edu] Sent: Friday, June 02, 2006 8:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura coverslipper Model 6400 Hi Histonetters, I'm demoing this automated glass coverslipper for two weeks. The person who currently repairs (frequently) my tape coverslippers said "if I think I have problems now, moving to a glass coverslipper will be even worse". What are your opinions? Thanks, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charles.Embrey <@t> carle.com Fri Jun 2 08:35:35 2006 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Fri Jun 2 08:35:28 2006 Subject: [Histonet] histotechs grossing and CLIA regs (again) Message-ID: CLIA regs apply to any lab that bills government insurance (Medicare, Medicaid, Tricare) or does work for any clinic or pathologist that bills those companies. One point is that your #1 response is slightly flawed. The person doesn't actually have to have the Associate Degree if they have the minimum collage hours in certain areas. The long answer from CLIA '88 is: 1. An earned associate degree in a laboratory science or medical laboratory technology, obtained from an accredited institution, OR 2. Education/training equivalent to the above that includes at least 60 semester hours or equivalent from an accredited institution. This education must include 24 semester hours of medical laboratory technology courses, OR 24 semester hours of science courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination. In addition, the individual must have laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, the CAHEA, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above), OR at least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. As to Florida license I would guess it would require at least an HT but that is only a guess. I would have to defer that answer to someone more versed in Florida law. Good Luck Charles Embrey Jr., PA(ASCP) www.GreyRealm.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ron Martin Sent: Thursday, June 01, 2006 7:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] histotechs grossing and CLIA regs (again) Fellow techs, I know this topic has been mentioned several times in the past but here I go again. I think the general interpretation ( from previous postings) on CLIA regs for histotechs grossing is the following: 1) After 1995 a person must have a minmum of a AS degree in a science ( Bio, Chem, Biochem, MLT, etc). 2) Before 1995 no degree but documented training in grossing. My questions are: Do these CLIA regs apply to all independent labs including POL's (Physician Office Labs)? I am currently with a POL derm lab and this question has come up. Also, in the state of Florida, is a state of Florida tech license required to gross ( in my case all derms)? Sorry to rehash old topics but I just need some additional input. Thanks, Ron Martin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Fri Jun 2 08:43:20 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Jun 2 08:42:59 2006 Subject: [Histonet] Whole brain perfusion of mice In-Reply-To: References: Message-ID: <44804078.8010503@umdnj.edu> Hi Andrew: 1. If you want "the opportunity to image a whole intact brain" with CT, why fix at all? What artifacts, if any, does fixation induce? Maybe fixation lowers contrast? Have you looked at a live mouse brain with CT? When CT is done on human brains, good contrast is obtained without fixation. 2. Osmium does not penetrate tissue well, 1 mm deep at best so even perfusing won't do much good. And how do you know osmium will increase the contrast with CT? Geoff Mr Andrew Tuck wrote: >Hi > >I was looking to use micro CT (computer tomography X ray) to examine >newborn (P0) mice brains. The area we are most interested in is >measuring the ventricle size in these animals. However we also want to >look at the size and shape of other regions such as fiber tracts, >etc..In the past this was done by doing 50um slices, putting these on >slides, photographing the slices and using software to calculate the >ventricle size. > >Using micro CT offers the opportunity to image a whole intact brain. >However, in the first scan we performed of a fixed mouse brain you could >see the shaodw outline of the brain but no internal detail. Osmium >tetroxide is the heavy metal of choice to produce good X ray contrast, >but does anyone know how you can perfuse an entire intact brain with it >? I have not been able to find any protocols, or previous articles which >have done this. Also I have read that osmium does not penetrate whole >tissue very well. If this is the case, is there another fixative that >would give good X ray contrast and penetrate the tissue well? > > >Thanks > >Andrew Tuck >Research Assistant >Queensland Centre for Schizophrenia Research >School of Biomedical Sciences >University of Queensland >Australia >Tel: 07 3346 2368 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From mcauliff <@t> umdnj.edu Fri Jun 2 08:51:25 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Jun 2 08:50:58 2006 Subject: [Histonet] section adhesion on slides In-Reply-To: <1149237104.447ff770acb17@mail.ugent.be> References: <1149237104.447ff770acb17@mail.ugent.be> Message-ID: <4480425D.5040605@umdnj.edu> Hi Olivier: Coat your own slides. Here is the method I use. "Silane" or APES coating <> Excellent for sections that will be subjected to trypsin digestion or other harsh treatments (saponification). Add 2 ml of 3-aminopropyltriethoxysilane (Sigma A-3648) to 98 ml of acetone. Dip clean slides in this mixture for 5 seconds, drain and rinse in two changes of acetone, 1 minute each. After about 35-40 slides discard the first acetone, move the second rinse up to be the first rinse and make a fresh second rinse. Air dry the slides in a vertical position and store in a dust free box. Keeps indefinitely. <> Rentrop, M., B. Knapp, H. Winter and J. Schweizer. Aminoalkylsilane-treated slides as support for in situ hybridization of keratin cDNA's to frozen tissue sections under varying fixation and pretreatment conditions. Histochemical J. 18:271-276, 1986. Note that clean is in bold type. I have never trusted "precleaned" slides, probably because I am old enough to remember when precleaned meant the absence of large pieces of dirt and not much else. I always wash my precleaned slides in hot, soapy water, rinse thoroughly, rinse in distilled water, dry in a clean (not paraffin) oven. Then coat with silane, albumin, gelatin, poly-L-lysine, etc. Geoff Olivier Leroux wrote: >Hello, > >Currently we are using vectabond coating to treat our slides for a good adhesion >of technovit 7100 sections. Does anybody know a cheaper alternative? > >regards, > >Olivier > >-- >Olivier Leroux >Ghent University >Department of Biology - Pteridological Section >K.L. Ledeganckstraat 35 >B-9000 Belgium > >http://www.pteridology.ugent.be >Olivier.Leroux@UGent.be > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From mcauliff <@t> umdnj.edu Fri Jun 2 08:53:47 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Jun 2 08:53:16 2006 Subject: [Histonet] Whole brain perfusion of mice again In-Reply-To: References: Message-ID: <448042EB.2050108@umdnj.edu> One more thing. Since a newborn mouse brain is not fully developed, don't expect to resolve the same anatomy seen in an adult brain. Geoff Mr Andrew Tuck wrote: >Hi > >I was looking to use micro CT (computer tomography X ray) to examine >newborn (P0) mice brains. The area we are most interested in is >measuring the ventricle size in these animals. However we also want to >look at the size and shape of other regions such as fiber tracts, >etc..In the past this was done by doing 50um slices, putting these on >slides, photographing the slices and using software to calculate the >ventricle size. > >Using micro CT offers the opportunity to image a whole intact brain. >However, in the first scan we performed of a fixed mouse brain you could >see the shaodw outline of the brain but no internal detail. Osmium >tetroxide is the heavy metal of choice to produce good X ray contrast, >but does anyone know how you can perfuse an entire intact brain with it >? I have not been able to find any protocols, or previous articles which >have done this. Also I have read that osmium does not penetrate whole >tissue very well. If this is the case, is there another fixative that >would give good X ray contrast and penetrate the tissue well? > > >Thanks > >Andrew Tuck >Research Assistant >Queensland Centre for Schizophrenia Research >School of Biomedical Sciences >University of Queensland >Australia >Tel: 07 3346 2368 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From cwscouten <@t> myneurolab.com Fri Jun 2 09:04:28 2006 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Fri Jun 2 09:04:48 2006 Subject: [Histonet] Whole brain perfusion of mice Message-ID: <5784D843593D874C93E9BADCB87342AB0130702D@tpiserver03.Coretech-holdings.com> The ventricle size changes dramatically with traditional perfusion, and some with any perfusion or with anoxia. If measuring ventrical size is the goal, you must work with living breathing animals. Blood flow gets very close to every cell in the brain, and so perfusion would get any perfused substance very close, within a mm surely, to any cell in the brain. Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Geoff McAuliffe Sent: Friday, June 02, 2006 8:43 AM To: Mr Andrew Tuck Cc: Histonet List Subject: Re: [Histonet] Whole brain perfusion of mice Hi Andrew: 1. If you want "the opportunity to image a whole intact brain" with CT, why fix at all? What artifacts, if any, does fixation induce? Maybe fixation lowers contrast? Have you looked at a live mouse brain with CT? When CT is done on human brains, good contrast is obtained without fixation. 2. Osmium does not penetrate tissue well, 1 mm deep at best so even perfusing won't do much good. And how do you know osmium will increase the contrast with CT? Geoff Mr Andrew Tuck wrote: >Hi > >I was looking to use micro CT (computer tomography X ray) to examine >newborn (P0) mice brains. The area we are most interested in is >measuring the ventricle size in these animals. However we also want to >look at the size and shape of other regions such as fiber tracts, >etc..In the past this was done by doing 50um slices, putting these on >slides, photographing the slices and using software to calculate the >ventricle size. > >Using micro CT offers the opportunity to image a whole intact brain. >However, in the first scan we performed of a fixed mouse brain you >could see the shaodw outline of the brain but no internal detail. >Osmium tetroxide is the heavy metal of choice to produce good X ray >contrast, but does anyone know how you can perfuse an entire intact >brain with it ? I have not been able to find any protocols, or previous >articles which have done this. Also I have read that osmium does not >penetrate whole tissue very well. If this is the case, is there another >fixative that would give good X ray contrast and penetrate the tissue well? > > >Thanks > >Andrew Tuck >Research Assistant >Queensland Centre for Schizophrenia Research School of Biomedical >Sciences University of Queensland Australia >Tel: 07 3346 2368 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DBaker <@t> sarapath.com Fri Jun 2 09:16:48 2006 From: DBaker <@t> sarapath.com (Dee Baker) Date: Fri Jun 2 09:16:29 2006 Subject: [Histonet] Liquid nitrogen procedure Message-ID: <211AEAE1E7C4974BB8B62A7BF8E4FF80063BA9@webber_nt.sarapath.com> We are currently looking into obtaining and using liquid nitrogen in our frozen section room. Does anyone have a procedure for the use and handling and use of liquid nitrogen. Thanks Dee **************************************************************************** SARASOTA PATHOLOGY CONFIDENTIALITY NOTICE: The information contained in this E-Mail message may be privileged, confidential, and protected under applicable law and is intended solely for the use of the individual or en- tity to whom it is addressed. If you are not the intended recipient or the employee or agent responsible for delivering the message to the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited. It is strictly prohi- bited from using the internet email system to send messages with patient identifiable information per HIPAA Privacy regulations and Sarasota Patho- logy policy. If you have received this communication in error, please notify the sender immediately and delete this message. **************************************************************************** From dsnider <@t> shrinenet.org Fri Jun 2 09:17:27 2006 From: dsnider <@t> shrinenet.org (Snider, Deanna) Date: Fri Jun 2 09:17:35 2006 Subject: [Histonet] Hacker HistoEDGE embedding center Message-ID: <84BE46B37B314D409C5A17B7BAB022D6925B18@IDC-EX-VS01.shriners.cc> Hello again. I am still researching embedding centers for our facility. I was recently contacted by Hacker Instruments and am going to demo this unit. Has anyone here ever used this one before? I had never heard of it until the Hacker rep contacted me. Deanna Snider HT ASCP Lead Histotechnician Research Laboratory Shriners Hospital for Children Cincinnati, Oh 513-872-6388 CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients. If you are not the intended recipient, (or authorized to receive for the recipient) you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please destroy all copies of this communication and any attachments and contact the sender by reply e-mail or telephone (813) 281-0300. From Bauer.Karen <@t> mayo.edu Fri Jun 2 09:32:07 2006 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen) Date: Fri Jun 2 09:32:58 2006 Subject: FW: [Histonet] Sakura coverslipper Model 6400 References: <3F500F8B416C554EBB21FF16642F72E959CEB1@marxchg03.mar.med.navy.mil> Message-ID: ________________________________ From: Bauer, Karen Sent: Fri 6/2/2006 9:31 AM To: Deltour, Douglas D. (HM2) Subject: RE: [Histonet] Sakura coverslipper Model 6400 Douglas, How does the tape last over the years? Right now, we coverslip manually, but have been thinking about getting a coverslipper in the near future. I've heard mixed emotions when it comes to tape or glass coverslips. We save our slides for 20 years. Does the tape stay adhered? I remember receiving a case a while ago that had tape coverslips on them and they were all falling off. Some of the patient tissues adhered to the coverslip so it created quite a mess trying to mount the rigid tape coverslip back onto the slide. Needless to say, they were not pretty when we got them back on. Just wondering, Karen Bauer HT(ASCP) ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Deltour, Douglas D. (HM2) Sent: Fri 6/2/2006 7:44 AM To: 'Angela Bitting'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Sakura coverslipper Model 6400 I will NEVER go back to glass. Our Sakura tissue-tek SCA coverslippers are workhorses. They have not needed service in over three years. The previous supervisor ordered a Tissue-Tek Glass coverslipper and it is collecting dust. It is not as fast as the tape and the techs feel the need to watch it as it coverslipps because they do not trust it. There are many myths and reasons that people do not like tape coverslippers. I will let the others get into those reasons but for my ulcer I would not have anything else but the tape. Good luck! Douglas Deltour HT(ASCP) -----Original Message----- From: Angela Bitting [mailto:akbitting@geisinger.edu] Sent: Friday, June 02, 2006 8:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura coverslipper Model 6400 Hi Histonetters, I'm demoing this automated glass coverslipper for two weeks. The person who currently repairs (frequently) my tape coverslippers said "if I think I have problems now, moving to a glass coverslipper will be even worse". What are your opinions? Thanks, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. From carolb <@t> phys.mcw.edu Fri Jun 2 09:36:05 2006 From: carolb <@t> phys.mcw.edu (Bobrowitz, Carol) Date: Fri Jun 2 09:36:09 2006 Subject: [Histonet] Martius Yellow Message-ID: <8F78639AC56F4143B267FE5F5A1B92C80E4304@guyton.phys.mcw.edu> I'm looking to purchase Martius Yellow CI #10315. Aldrich catalog # S480541, Martius Yellow is listed under Acid Yellow 24 CI#10315 and lists for 25 mg for $50.00. Aldrich catalog # 377767, Martius Yellow, dye content 85%, does not have a CI# and it lists for 25 grams for $39.30. Does it make a difference if you choose to use the Martius Yellow with no CI #? (Thanks Kathy J. for your information) The difference in price makes a difference. Thank you, Carol Ann Bobrowitz Department of Physiology - Histology Medical College of Wisconsin cbobrowi@mcw.edu From DDDeltour <@t> mar.med.navy.mil Fri Jun 2 09:47:58 2006 From: DDDeltour <@t> mar.med.navy.mil (Deltour, Douglas D. (HM2)) Date: Fri Jun 2 09:48:08 2006 Subject: [Histonet] Sakura coverslipper Model 6400 Message-ID: <3F500F8B416C554EBB21FF16642F72E959CEB4@marxchg03.mar.med.navy.mil> Karen, That is the biggest complaint that I hear from people. I actually have not seen this from any of our cases. We keep ours for ten years so I can not comment on twenty years. I have been told (from numerous people) that if you properly dehydrate and clear the slides then this does not happen. If the alcohols and xylenes on your stainer are changed regularly then the tape coming off is not an issue. I have also heard that if the slides are stored in a humid location that it may cause this issue. Douglas Deltour HT(ASCP) -----Original Message----- From: Bauer, Karen [mailto:Bauer.Karen@mayo.edu] Sent: Friday, June 02, 2006 10:32 AM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] Sakura coverslipper Model 6400 ________________________________ From: Bauer, Karen Sent: Fri 6/2/2006 9:31 AM To: Deltour, Douglas D. (HM2) Subject: RE: [Histonet] Sakura coverslipper Model 6400 Douglas, How does the tape last over the years? Right now, we coverslip manually, but have been thinking about getting a coverslipper in the near future. I've heard mixed emotions when it comes to tape or glass coverslips. We save our slides for 20 years. Does the tape stay adhered? I remember receiving a case a while ago that had tape coverslips on them and they were all falling off. Some of the patient tissues adhered to the coverslip so it created quite a mess trying to mount the rigid tape coverslip back onto the slide. Needless to say, they were not pretty when we got them back on. Just wondering, Karen Bauer HT(ASCP) ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Deltour, Douglas D. (HM2) Sent: Fri 6/2/2006 7:44 AM To: 'Angela Bitting'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Sakura coverslipper Model 6400 I will NEVER go back to glass. Our Sakura tissue-tek SCA coverslippers are workhorses. They have not needed service in over three years. The previous supervisor ordered a Tissue-Tek Glass coverslipper and it is collecting dust. It is not as fast as the tape and the techs feel the need to watch it as it coverslipps because they do not trust it. There are many myths and reasons that people do not like tape coverslippers. I will let the others get into those reasons but for my ulcer I would not have anything else but the tape. Good luck! Douglas Deltour HT(ASCP) -----Original Message----- From: Angela Bitting [mailto:akbitting@geisinger.edu] Sent: Friday, June 02, 2006 8:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura coverslipper Model 6400 Hi Histonetters, I'm demoing this automated glass coverslipper for two weeks. The person who currently repairs (frequently) my tape coverslippers said "if I think I have problems now, moving to a glass coverslipper will be even worse". What are your opinions? Thanks, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Fri Jun 2 09:47:21 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Jun 2 09:49:04 2006 Subject: [Histonet] Sakura coverslipper Model 6400 References: Message-ID: Believe your eyes - we got rid of our tape coverslipper and replaced it with the glass. Our slides from previous years usually need to be recut - a disaster for cases with levels! ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bauer, Karen Sent: Fri 6/2/2006 10:32 AM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] Sakura coverslipper Model 6400 ________________________________ From: Bauer, Karen Sent: Fri 6/2/2006 9:31 AM To: Deltour, Douglas D. (HM2) Subject: RE: [Histonet] Sakura coverslipper Model 6400 Douglas, How does the tape last over the years? Right now, we coverslip manually, but have been thinking about getting a coverslipper in the near future. I've heard mixed emotions when it comes to tape or glass coverslips. We save our slides for 20 years. Does the tape stay adhered? I remember receiving a case a while ago that had tape coverslips on them and they were all falling off. Some of the patient tissues adhered to the coverslip so it created quite a mess trying to mount the rigid tape coverslip back onto the slide. Needless to say, they were not pretty when we got them back on. Just wondering, Karen Bauer HT(ASCP) ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Deltour, Douglas D. (HM2) Sent: Fri 6/2/2006 7:44 AM To: 'Angela Bitting'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Sakura coverslipper Model 6400 I will NEVER go back to glass. Our Sakura tissue-tek SCA coverslippers are workhorses. They have not needed service in over three years. The previous supervisor ordered a Tissue-Tek Glass coverslipper and it is collecting dust. It is not as fast as the tape and the techs feel the need to watch it as it coverslipps because they do not trust it. There are many myths and reasons that people do not like tape coverslippers. I will let the others get into those reasons but for my ulcer I would not have anything else but the tape. Good luck! Douglas Deltour HT(ASCP) -----Original Message----- From: Angela Bitting [mailto:akbitting@geisinger.edu] Sent: Friday, June 02, 2006 8:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura coverslipper Model 6400 Hi Histonetters, I'm demoing this automated glass coverslipper for two weeks. The person who currently repairs (frequently) my tape coverslippers said "if I think I have problems now, moving to a glass coverslipper will be even worse". What are your opinions? Thanks, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Fri Jun 2 09:50:10 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Jun 2 09:51:47 2006 Subject: [Histonet] Sakura coverslipper Model 6400 References: Message-ID: I don't find this to be true - we have 1000 blocks (give or take 150 blocks) so we have a load of slides and real good luck with the Leica CV5030 coverslippers - we have three of them. ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Angela Bitting Sent: Fri 6/2/2006 8:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura coverslipper Model 6400 Hi Histonetters, I'm demoing this automated glass coverslipper for two weeks. The person who currently repairs (frequently) my tape coverslippers said "if I think I have problems now, moving to a glass coverslipper will be even worse". What are your opinions? Thanks, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Bauer.Karen <@t> mayo.edu Fri Jun 2 09:56:54 2006 From: Bauer.Karen <@t> mayo.edu (Bauer, Karen) Date: Fri Jun 2 09:57:35 2006 Subject: [Histonet] Sakura coverslipper Model 6400 References: <3F500F8B416C554EBB21FF16642F72E959CEB4@marxchg03.mar.med.navy.mil> Message-ID: Thanks Douglas, good to know. Karen ________________________________ From: Deltour, Douglas D. (HM2) [mailto:DDDeltour@mar.med.navy.mil] Sent: Fri 6/2/2006 9:47 AM To: 'Bauer, Karen'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Sakura coverslipper Model 6400 Karen, That is the biggest complaint that I hear from people. I actually have not seen this from any of our cases. We keep ours for ten years so I can not comment on twenty years. I have been told (from numerous people) that if you properly dehydrate and clear the slides then this does not happen. If the alcohols and xylenes on your stainer are changed regularly then the tape coming off is not an issue. I have also heard that if the slides are stored in a humid location that it may cause this issue. Douglas Deltour HT(ASCP) -----Original Message----- From: Bauer, Karen [mailto:Bauer.Karen@mayo.edu] Sent: Friday, June 02, 2006 10:32 AM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] Sakura coverslipper Model 6400 ________________________________ From: Bauer, Karen Sent: Fri 6/2/2006 9:31 AM To: Deltour, Douglas D. (HM2) Subject: RE: [Histonet] Sakura coverslipper Model 6400 Douglas, How does the tape last over the years? Right now, we coverslip manually, but have been thinking about getting a coverslipper in the near future. I've heard mixed emotions when it comes to tape or glass coverslips. We save our slides for 20 years. Does the tape stay adhered? I remember receiving a case a while ago that had tape coverslips on them and they were all falling off. Some of the patient tissues adhered to the coverslip so it created quite a mess trying to mount the rigid tape coverslip back onto the slide. Needless to say, they were not pretty when we got them back on. Just wondering, Karen Bauer HT(ASCP) ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Deltour, Douglas D. (HM2) Sent: Fri 6/2/2006 7:44 AM To: 'Angela Bitting'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Sakura coverslipper Model 6400 I will NEVER go back to glass. Our Sakura tissue-tek SCA coverslippers are workhorses. They have not needed service in over three years. The previous supervisor ordered a Tissue-Tek Glass coverslipper and it is collecting dust. It is not as fast as the tape and the techs feel the need to watch it as it coverslipps because they do not trust it. There are many myths and reasons that people do not like tape coverslippers. I will let the others get into those reasons but for my ulcer I would not have anything else but the tape. Good luck! Douglas Deltour HT(ASCP) -----Original Message----- From: Angela Bitting [mailto:akbitting@geisinger.edu] Sent: Friday, June 02, 2006 8:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura coverslipper Model 6400 Hi Histonetters, I'm demoing this automated glass coverslipper for two weeks. The person who currently repairs (frequently) my tape coverslippers said "if I think I have problems now, moving to a glass coverslipper will be even worse". What are your opinions? Thanks, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. From Nancy.Temple <@t> ssfhs.org Fri Jun 2 10:06:52 2006 From: Nancy.Temple <@t> ssfhs.org (Temple Nancy) Date: Fri Jun 2 10:07:11 2006 Subject: [Histonet] Sakura coverslipper Model 6400 Message-ID: I have used both tape coverslipper and glass (Leica) coverslipper. We(especially our pathologists)prefer the glass coverslips over tape. Nancy Temple HT(ASCP) Histology/Cytology Supervisor St. Francis Hospital Indianapolis, In -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, June 02, 2006 8:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura coverslipper Model 6400 Hi Histonetters, I'm demoing this automated glass coverslipper for two weeks. The person who currently repairs (frequently) my tape coverslippers said "if I think I have problems now, moving to a glass coverslipper will be even worse". What are your opinions? Thanks, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From limla <@t> mail.nih.gov Fri Jun 2 10:09:54 2006 From: limla <@t> mail.nih.gov (Lim, Langston (NIH/NCI) [E]) Date: Fri Jun 2 10:09:58 2006 Subject: FW: [Histonet] Sakura coverslipper Model 6400 Message-ID: Langston Lim, HT (ASCP) Tissue Array Research Program DHHS/NIH/NCI/CCR/LP 301-402-4927 -----Original Message----- From: Lim, Langston (NIH/NCI) [E] Sent: Friday, June 02, 2006 11:08 AM To: 'Bonner, Janet' Subject: RE: [Histonet] Sakura coverslipper Model 6400 I agree with Karen. Tape coverslips are a pain when it comes to recoverslipping (tissues sticking to the coverslip) and do fall off in the long run and not good for imaging either because of the glare. Also performing a tissue transfer is just about impossible. Langston Lim, HT (ASCP) Tissue Array Research Program DHHS/NIH/NCI/CCR/LP -----Original Message----- From: Bonner, Janet [mailto:Janet.Bonner@FLHOSP.ORG] Sent: Friday, June 02, 2006 10:47 AM To: Bauer, Karen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Sakura coverslipper Model 6400 Believe your eyes - we got rid of our tape coverslipper and replaced it with the glass. Our slides from previous years usually need to be recut - a disaster for cases with levels! ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bauer, Karen Sent: Fri 6/2/2006 10:32 AM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] Sakura coverslipper Model 6400 ________________________________ From: Bauer, Karen Sent: Fri 6/2/2006 9:31 AM To: Deltour, Douglas D. (HM2) Subject: RE: [Histonet] Sakura coverslipper Model 6400 Douglas, How does the tape last over the years? Right now, we coverslip manually, but have been thinking about getting a coverslipper in the near future. I've heard mixed emotions when it comes to tape or glass coverslips. We save our slides for 20 years. Does the tape stay adhered? I remember receiving a case a while ago that had tape coverslips on them and they were all falling off. Some of the patient tissues adhered to the coverslip so it created quite a mess trying to mount the rigid tape coverslip back onto the slide. Needless to say, they were not pretty when we got them back on. Just wondering, Karen Bauer HT(ASCP) ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Deltour, Douglas D. (HM2) Sent: Fri 6/2/2006 7:44 AM To: 'Angela Bitting'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Sakura coverslipper Model 6400 I will NEVER go back to glass. Our Sakura tissue-tek SCA coverslippers are workhorses. They have not needed service in over three years. The previous supervisor ordered a Tissue-Tek Glass coverslipper and it is collecting dust. It is not as fast as the tape and the techs feel the need to watch it as it coverslipps because they do not trust it. There are many myths and reasons that people do not like tape coverslippers. I will let the others get into those reasons but for my ulcer I would not have anything else but the tape. Good luck! Douglas Deltour HT(ASCP) -----Original Message----- From: Angela Bitting [mailto:akbitting@geisinger.edu] Sent: Friday, June 02, 2006 8:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura coverslipper Model 6400 Hi Histonetters, I'm demoing this automated glass coverslipper for two weeks. The person who currently repairs (frequently) my tape coverslippers said "if I think I have problems now, moving to a glass coverslipper will be even worse". What are your opinions? Thanks, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From froyer <@t> bitstream.net Fri Jun 2 10:18:31 2006 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Jun 2 10:18:38 2006 Subject: [Histonet] Sakura coverslipper Model 6400 In-Reply-To: Message-ID: <006201c68657$cef00130$7701a80a@Ford> -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Temple Nancy Sent: Friday, June 02, 2006 10:07 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Sakura coverslipper Model 6400 I have used both tape coverslipper and glass (Leica) coverslipper. We(especially our pathologists)prefer the glass coverslips over tape. Nancy Temple HT(ASCP) Histology/Cytology Supervisor St. Francis Hospital Indianapolis, In ---------------------------------------- So... you first put on a tape coverslip and then add a glass coverslip? Is this done manually or by sequential automated coverslippers? Isn't this a little redundant? Or is it that your pathologists are into double jeopardy? ;-) ...sorry couldn't resist. (LOL) ~ Ford Ford M. Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. Golden Valley, MN 55427-3601 From vazquezr <@t> ohsu.edu Fri Jun 2 10:35:37 2006 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Jun 2 10:39:19 2006 Subject: [Histonet] Liquid nitrogen procedure Message-ID: Wear eye glasses and gloves when handling. Don't stand against the wall when filling your cryogun, wear long sleeves. Be careful of splashes and misfires of gun. If you are sitting at a cryostat and you place the gun on the top, make sure the nozzle is facing away from you or anyone else. Not to offend any rocket scientist :>)but you don't have to be a rocket scientist, just use common sense and caution. Have a great day and weekend. Robyn OHSU From DeBrosse_Beatrice <@t> Allergan.com Fri Jun 2 10:42:38 2006 From: DeBrosse_Beatrice <@t> Allergan.com (DeBrosse_Beatrice) Date: Fri Jun 2 10:42:59 2006 Subject: [Histonet] Sakura coverslipper Model 6400 Message-ID: We have a Sakura glass coverslipper and are semi-happy with it. Some days it works perfectly fine, on others anything but. Beatrice DeBrosse-Serra HT(ASCP)QIHC Allergan, Inc. 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5116 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, June 02, 2006 5:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura coverslipper Model 6400 Hi Histonetters, I'm demoing this automated glass coverslipper for two weeks. The person who currently repairs (frequently) my tape coverslippers said "if I think I have problems now, moving to a glass coverslipper will be even worse". What are your opinions? Thanks, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From CBono <@t> epl-inc.com Fri Jun 2 11:04:03 2006 From: CBono <@t> epl-inc.com (Cyndi Bono) Date: Fri Jun 2 11:02:41 2006 Subject: [Histonet] Looking for Mary Gessford Message-ID: Hi all. I'm trying to contact Mary Gessford. If anyone knows where to find her, let me know or tell her to contact me. Thanks Cyndi Bono, HT(ASCP) Client Services Director EPL, Inc. PO Box 474 Herndon, VA 20172-0474 Tel: 703-471-7060 X221 Fax: 703-471-8447 email: cbono@epl-inc.com From Jackie.O'Connor <@t> abbott.com Fri Jun 2 11:14:01 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Jun 2 11:14:29 2006 Subject: [Histonet] TMA help request In-Reply-To: <026401c68590$0d38aa00$27955c82@patho.unibe.ch> Message-ID: I'm doing TMA's using a Beecher manual arrayer. I basically taught myself how to do it, and I need some advice on my technique. Is there anyone in the Chicago/Northern Illinois/Southern Wisconsin area who would be willing to let me stop by and observe your TMA assembly process? Thanks. Jackie O'Connor From sluhisto <@t> yahoo.com Fri Jun 2 11:21:52 2006 From: sluhisto <@t> yahoo.com (Histology SLU) Date: Fri Jun 2 11:21:58 2006 Subject: [Histonet] Ventant Benchmark In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD320263E11C@sjhaexc02.sjha.org> Message-ID: <20060602162152.5390.qmail@web51006.mail.yahoo.com> Dito to Joyce's reply. We also are on the list to get the newest Dako Technology, the Eridan, hopefully by the end of summer. And that one IS a true walk away. It will deparaffinize thru the end of staining. And it is continuous load. But is still open. Susan "Weems, Joyce" wrote: That's what I was saying too. Even with all our cost cutting attempts, do enough immunos to support 3 reagent-leased DAKO instruments. They're walk away too = after you get them loaded anyway! - and the reagents are not nearly as expensive. We titer all the antibodies that are available - a savings over the prepared Abs. The company has been a great partner for our laboratory. j Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Joe Nocito Sent: Sat 5/27/2006 6:58 PM To: Malcolm McCallum; Scott A. Ely; histonet@lists.utsouthwestern.edu Cc: histonet-owner@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventant Benchmark I'm not saying do it manually. I'm saying that there are less expensive ways to perform immunos than using Ventana's high cost reagents. Joe ----- Original Message ----- From: "Malcolm McCallum" To: "Joe Nocito" ; "Scott A. Ely" ; Cc: Sent: Saturday, May 27, 2006 10:39 AM Subject: RE: [Histonet] Ventant Benchmark Undoubtedly the cost and maintenance of automation is cheaper than the cost of additional employees salaries and benefits accompanied by associated duplication of manual systems. Additionally, equipment is depreciable, whereas human resources are a cost. VISIT THE JOURNAL HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-223-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Joe Nocito Sent: Sat 5/27/2006 8:29 AM To: Scott A. Ely; histonet@lists.utsouthwestern.edu Cc: histonet-owner@lists.utsouthwestern.edu Subject: Re: [Histonet] Ventant Benchmark flaming time How come every one talks about the reliability and walk away technology, but no one ever talks about the price of running these machines? Shouldn't cost be a factor also? I've been in budget meetings all week. The only thing I heard was how expensive it is to run immunos. Yeah, we have two XTs, what do you expect? The opinions stated here are that of the author only and does not express the opinions of his employer, lawyers, siblings, significant others and their lawyers. Oh, by the way, I told the same thing to my Ventana Rep on Friday. He knows, so y'all don't need to call my CEO. Y'all know who you are. Let the flaming begin. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Scott A. Ely" To: Cc: Sent: Friday, May 26, 2006 11:58 AM Subject: [Histonet] Ventant Benchmark > We are considering buying some new equipment. I would like to know who > uses the Ventana Benchmark > immunostainer or other immunostainers and what you like and don't like > about it. > > Scott Ely, MD MPH > Section of Hematopathology > Department of Pathology > Weill Medical College of Cornell University > New York Presbyterian Hospital > 525 E. 68th Street > New York, NY 10021 > PH: 212-746-2442 > > Legal Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of the original > intended recipient(s) selected by Dr. Ely and may contain confidential and > privileged information. Any > unauthorized review, use, disclosure or distribution is prohibited. If you > are not the recipient specified by Dr. > Ely, please contact the sender by reply e-mail and destroy all copies of > the original message. > > ----- Original Message ----- > From: histonet-request@lists.utsouthwestern.edu > Date: Friday, May 26, 2006 12:29 pm > Subject: Histonet Digest, Vol 30, Issue 39 > > -------------------------------------------------------------------------------- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -------------------------------------------------------------------------------- > -------------------------------------------------------------------------------- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.7.1/348 - Release Date: 5/25/2006 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.7.2/349 - Release Date: 5/26/2006 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From melaniepride <@t> hotmail.com Fri Jun 2 11:22:13 2006 From: melaniepride <@t> hotmail.com (melanie pride) Date: Fri Jun 2 11:22:24 2006 Subject: [Histonet] Sakura coverslipper Model 6400 In-Reply-To: Message-ID: I agreee with the automated coverslipper. I have had good luck with it in the past, and if you do a lot of coverslipping, its a dream come true ;) P. Melanie Pride Department of Surgery IU School of Medicine [1]ppride@iupui.edu ______________________________________________________________ From: "Bonner, Janet" To: "Angela Bitting" ,histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Sakura coverslipper Model 6400 Date: Fri, 2 Jun 2006 10:50:10 -0400 >I don't find this to be true - we have 1000 blocks (give or take 150 blocks) so we have a load of slides and real good luck with the Leica CV5030 coverslippers - we have three of them. > >________________________________ > >From: histonet-bounces@lists.utsouthwestern.edu on behalf of Angela Bitting >Sent: Fri 6/2/2006 8:20 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Sakura coverslipper Model 6400 > > > >Hi Histonetters, > >I'm demoing this automated glass coverslipper for two weeks. The person who currently repairs (frequently) my tape coverslippers said "if I think I have problems now, moving to a glass coverslipper will be even worse". > >What are your opinions? > >Thanks, >Angie > >Angela Bitting, HT(ASCP) >Technical Specialist, Histology >Geisinger Medical Center >100 N Academy Ave. MC 23-00 >Danville, PA 17822 >phone 570-214-9634 >fax 570-271-5916 > > > >IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet References Visible links 1. mailto:ppride@iupui.edu Hidden links: 2. http://www.americanmessaging.net/sendpage_1way.html From HornHV <@t> archildrens.org Fri Jun 2 11:38:06 2006 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Jun 2 11:38:36 2006 Subject: [Histonet] Sakura coverslipper Model 6400 Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C63038BE0CE@EMAIL.archildrens.org> I, too, have seen several cases with the tape coverslip coming off from slides sent to us. And the worst part is the tissue is adhered to the coverslip and not the slide. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen Sent: Friday, June 02, 2006 9:57 AM To: Deltour, Douglas D. (HM2); Bauer, Karen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Sakura coverslipper Model 6400 Thanks Douglas, good to know. Karen ________________________________ From: Deltour, Douglas D. (HM2) [mailto:DDDeltour@mar.med.navy.mil] Sent: Fri 6/2/2006 9:47 AM To: 'Bauer, Karen'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Sakura coverslipper Model 6400 Karen, That is the biggest complaint that I hear from people. I actually have not seen this from any of our cases. We keep ours for ten years so I can not comment on twenty years. I have been told (from numerous people) that if you properly dehydrate and clear the slides then this does not happen. If the alcohols and xylenes on your stainer are changed regularly then the tape coming off is not an issue. I have also heard that if the slides are stored in a humid location that it may cause this issue. Douglas Deltour HT(ASCP) -----Original Message----- From: Bauer, Karen [mailto:Bauer.Karen@mayo.edu] Sent: Friday, June 02, 2006 10:32 AM To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] Sakura coverslipper Model 6400 ________________________________ From: Bauer, Karen Sent: Fri 6/2/2006 9:31 AM To: Deltour, Douglas D. (HM2) Subject: RE: [Histonet] Sakura coverslipper Model 6400 Douglas, How does the tape last over the years? Right now, we coverslip manually, but have been thinking about getting a coverslipper in the near future. I've heard mixed emotions when it comes to tape or glass coverslips. We save our slides for 20 years. Does the tape stay adhered? I remember receiving a case a while ago that had tape coverslips on them and they were all falling off. Some of the patient tissues adhered to the coverslip so it created quite a mess trying to mount the rigid tape coverslip back onto the slide. Needless to say, they were not pretty when we got them back on. Just wondering, Karen Bauer HT(ASCP) ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Deltour, Douglas D. (HM2) Sent: Fri 6/2/2006 7:44 AM To: 'Angela Bitting'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Sakura coverslipper Model 6400 I will NEVER go back to glass. Our Sakura tissue-tek SCA coverslippers are workhorses. They have not needed service in over three years. The previous supervisor ordered a Tissue-Tek Glass coverslipper and it is collecting dust. It is not as fast as the tape and the techs feel the need to watch it as it coverslipps because they do not trust it. There are many myths and reasons that people do not like tape coverslippers. I will let the others get into those reasons but for my ulcer I would not have anything else but the tape. Good luck! Douglas Deltour HT(ASCP) -----Original Message----- From: Angela Bitting [mailto:akbitting@geisinger.edu] Sent: Friday, June 02, 2006 8:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura coverslipper Model 6400 Hi Histonetters, I'm demoing this automated glass coverslipper for two weeks. The person who currently repairs (frequently) my tape coverslippers said "if I think I have problems now, moving to a glass coverslipper will be even worse". What are your opinions? Thanks, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From c.ingles <@t> hosp.wisc.edu Fri Jun 2 11:38:43 2006 From: c.ingles <@t> hosp.wisc.edu (Ingles Claire) Date: Fri Jun 2 11:38:48 2006 Subject: [Histonet] Sakura coverslipper Model 6400 References: Message-ID: <583D3E9A1E843445BD54E461D1A2F6F317A04F55@UWHIS-XCHNG2.uwhis.hosp.wisc.edu> I have worked with both tape and glass. Both have had problems and both have their advantages. Yes, tape is faster and has fewer bubbles, but we had problems with ours too. We used to have to keep adjusting the flow of xylene, sometimes several times a day. The tape rolls are really expensive, and they tend to start peeling away from the slide after a while and they have to be re-coverslipped again. Glass (Leica) doesn't really have trouble with bubbles, but sometimes the coverslips stick together. We don't worry about walking away from it. Drawbacks are that it does go a bit slower, but still faster than hand coverslipping. Also, only one rack of slides can be put on at a time. Claire Ingles Mohs Clinic UW Hospital & Clinics Madison WI ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Angela Bitting Sent: Fri 6/2/2006 7:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura coverslipper Model 6400 Hi Histonetters, I'm demoing this automated glass coverslipper for two weeks. The person who currently repairs (frequently) my tape coverslippers said "if I think I have problems now, moving to a glass coverslipper will be even worse". What are your opinions? Thanks, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JSCHUMA1 <@t> Fairview.org Fri Jun 2 11:51:43 2006 From: JSCHUMA1 <@t> Fairview.org (Schumacher, Jennifer J) Date: Fri Jun 2 11:51:50 2006 Subject: [Histonet] Sakura coverslipper Model 6400 Message-ID: I agree. The tape coverslipper was faster and more of a workhorse than the Sakura glass model. But, the pathologists prefer glass and the slides look better when you have to go back to them. Our Sakura glass coverslipper works well some days, and is problematic on others. It takes time to get to know the quirks and causes, and sometimes the position of the moon seems to affect it . . . Jennifer Schumacher CLS Lead, Pathology Riverside Campus University of Minnesota Medical Center, Fairview 2450 Riverside Ave Minneapolis, MN 55454 Phone 612.273.2883 Pager 612.899.6038 Fax 612.273.9124 email jschuma1@fairview.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DeBrosse_Beatrice Sent: Friday, June 02, 2006 10:43 AM To: Angela Bitting; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Sakura coverslipper Model 6400 We have a Sakura glass coverslipper and are semi-happy with it. Some days it works perfectly fine, on others anything but. Beatrice DeBrosse-Serra HT(ASCP)QIHC Allergan, Inc. 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5116 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Friday, June 02, 2006 5:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura coverslipper Model 6400 Hi Histonetters, I'm demoing this automated glass coverslipper for two weeks. The person who currently repairs (frequently) my tape coverslippers said "if I think I have problems now, moving to a glass coverslipper will be even worse". What are your opinions? Thanks, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lrichey <@t> u.washington.edu Fri Jun 2 11:51:57 2006 From: lrichey <@t> u.washington.edu (Lori Richey) Date: Fri Jun 2 11:52:27 2006 Subject: [Histonet] Sakura coverslipper Model 6400 In-Reply-To: References: Message-ID: <44806CAD.2080808@u.washington.edu> The glass coverslipper works well.It requires daily cleaning, whether it is alarming or not. If the maintenance is done daily, its a good coverslipper. The pathologists love the glass. The plastic doesn't preserve the section very well. Often times, the section will come off the slide onto the plastic coverslip after a few years. Angela Bitting wrote: >Hi Histonetters, > >I'm demoing this automated glass coverslipper for two weeks. The person who currently repairs (frequently) my tape coverslippers said "if I think I have problems now, moving to a glass coverslipper will be even worse". > >What are your opinions? > >Thanks, >Angie > >Angela Bitting, HT(ASCP) >Technical Specialist, Histology >Geisinger Medical Center >100 N Academy Ave. MC 23-00 >Danville, PA 17822 >phone 570-214-9634 >fax 570-271-5916 > > > >IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From jqb7 <@t> cdc.gov Fri Jun 2 11:49:19 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/NCID/VR)) Date: Fri Jun 2 11:58:15 2006 Subject: [Histonet] Sakura coverslipper Model 6400 Message-ID: The new Leica allows for 2 or 3 racks of slides to be loaded at once. Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Friday, June 02, 2006 12:39 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Sakura coverslipper Model 6400 I have worked with both tape and glass. Both have had problems and both have their advantages. Yes, tape is faster and has fewer bubbles, but we had problems with ours too. We used to have to keep adjusting the flow of xylene, sometimes several times a day. The tape rolls are really expensive, and they tend to start peeling away from the slide after a while and they have to be re-coverslipped again. Glass (Leica) doesn't really have trouble with bubbles, but sometimes the coverslips stick together. We don't worry about walking away from it. Drawbacks are that it does go a bit slower, but still faster than hand coverslipping. Also, only one rack of slides can be put on at a time. Claire Ingles Mohs Clinic UW Hospital & Clinics Madison WI ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Angela Bitting Sent: Fri 6/2/2006 7:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura coverslipper Model 6400 Hi Histonetters, I'm demoing this automated glass coverslipper for two weeks. The person who currently repairs (frequently) my tape coverslippers said "if I think I have problems now, moving to a glass coverslipper will be even worse". What are your opinions? Thanks, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Fri Jun 2 12:06:24 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Jun 2 12:06:26 2006 Subject: AW: [Histonet] att: Bryan D. Llewellyn In-Reply-To: <8F78639AC56F4143B267FE5F5A1B92C80E4301@guyton.phys.mcw.edu> Message-ID: <000001c68666$e141f460$eeeea8c0@SERVER01> Conns Biological Stains: Martius Yellow CI 10315 for MSB Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Bobrowitz, Carol Gesendet: Donnerstag, 01. Juni 2006 21:31 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] att: Bryan D. Llewellyn Hello Bryan, My co-worker just brought in a copy of a special stain, MSB for Fibrin, copied from the StainsFile site. As I was gathering information I noticed when I clicked on Martius Yellow CI# 10315 its shows up as Fast Yellow CI#13015. I was wondering which ??? Yellow is required to do the MSB stain. Fast Yellow, CI# 13015, is used in the Wallart & Honette's Trichrome stain. Your help will be appreciated Thank you, Carol Ann Bobrowitz Department of Physiology - Histology Medical College of Wisconsin cbobrowi@mcw.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Fri Jun 2 12:20:51 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Jun 2 12:20:48 2006 Subject: AW: [Histonet] Sakura coverslipper Model 6400 In-Reply-To: Message-ID: <000101c68668$e599f1f0$eeeea8c0@SERVER01> We had a tape coverslipper, tried the Sakura glass coverslipper and bought the Leica coverslipper in combination with the stainer. It was a good choice. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Angela Bitting Gesendet: Freitag, 02. Juni 2006 14:20 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Sakura coverslipper Model 6400 Hi Histonetters, I'm demoing this automated glass coverslipper for two weeks. The person who currently repairs (frequently) my tape coverslippers said "if I think I have problems now, moving to a glass coverslipper will be even worse". What are your opinions? Thanks, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From quinntl <@t> umkc.edu Fri Jun 2 12:22:45 2006 From: quinntl <@t> umkc.edu (Quinn, Tim L.) Date: Fri Jun 2 12:22:52 2006 Subject: [Histonet] Bulk DAB Message-ID: Dear Listies, I would like to start using 250 mL tubs for DAB staining to get staining time standardized. Is there a source for large purchase of reagents at an affordable price? Cheers, Timothy L. Quinn Senior Lab Technician Missouri University at Kansas City Medical School- Med Lab 2411 Holmes Kansas City, MO 64108 816-235-1904 quinntl@umkc.edu From sbreeden <@t> nmda.nmsu.edu Fri Jun 2 13:33:50 2006 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Jun 2 13:33:54 2006 Subject: [Histonet] Tape Coverslippers Rule! Message-ID: Long Live Tape! I've been monitoring several of these tape vs glass conversations and I notice some salient points are missing from the debate: first, tape requires xylene - no substitutes; if your drip rate is 4-5 drips per slide, the tape adheres well; finally, if you must remove the coverslip, submerge the slide in acetone for 5-10 minutes and the tape shrivels and can be lifted off without removing the tissue; then run back thru xylene and re-cover (or whatever). The glass coverslippers give me the willies - watching all those minutely adjustable parts whipping around....brrrr..... All you Glass Coverslipper Devotees - I'm wearing my flameproof 'jammies - go for it! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87108 From Reuel.Cornelia <@t> tsrh.org Fri Jun 2 12:49:59 2006 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Fri Jun 2 14:59:37 2006 Subject: [Histonet] looking for a new tissue processor Message-ID: I just wanted to hear from anyone what tissue processor would you like to recommend to change our Shandon hypercenter XP. We have our machine for 12 years now and it is still running smoothly without any problem.Shandon Service contract will discontinue to service this machine which has been running for 7 years although we found contractors who are still willing to maintain our machine. I just wanted a feedback and I will look into it. Thank you. What can one mind do without histonet? ******************************************************************************************************************* Texas Scottish Rite Hospital for Children is one of the nation's leading pediatric centers for the treatment of orthopedic conditions and learning disorders, like dyslexia. This email transmission and/or its attachments may contain confidential health information, intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing it to any other party unless required to do so by law and is required to delete/destroy the information after its stated need has been fulfilled. If you are not the intended recipient, any disclosure, copying, distribution or action taken in reliance on the contents of this email transmission is prohibited. If you have received this information in error, please notify the sender immediately and delete this information. We appreciate your efforts to protect the children's confidential information. ******************************************************************************************************************* From histo20 <@t> hotmail.com Fri Jun 2 15:13:01 2006 From: histo20 <@t> hotmail.com (Paula Wilder) Date: Fri Jun 2 15:13:09 2006 Subject: [Histonet] Scoring of ER/PR and ISH Message-ID: Hello Histonet! Can anyone help me with this CAP question? Our pathologist would like to know how everyone scores their ER/PRs and ISH. Any help would be greatly appreciated!!! Paula Wilder St. Joseph Medical Center Towson, MD 21204 From mgdelaware <@t> comcast.net Fri Jun 2 15:15:46 2006 From: mgdelaware <@t> comcast.net (Marian Powers) Date: Fri Jun 2 15:15:52 2006 Subject: [Histonet] MCL CONTROL SLIDES Message-ID: <000801c68681$55d84da0$3227c847@D7XQNX91> Hello, looking to purchase mantle cell lymphoma control slides, any recommendations? Thanks in advance, Marian From mgdelaware <@t> comcast.net Fri Jun 2 15:17:26 2006 From: mgdelaware <@t> comcast.net (Marian Powers) Date: Fri Jun 2 15:17:30 2006 Subject: [Histonet] Laminin Message-ID: <000f01c68681$912b8750$3227c847@D7XQNX91> Hello, We are unable to validate Dako Laminin with Ventana. Any one successful and willing to share your protocol. Thanks in advance, Marian From Rcartun <@t> harthosp.org Fri Jun 2 15:40:55 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Jun 2 15:41:35 2006 Subject: [Histonet] Scoring of ER/PR and ISH In-Reply-To: References: Message-ID: <44806A1702000077000004CD@hcnwgwds01.hh.chs> We use Dr. Craig Allred's scoring system (see Mod Pathol 1998;11(2):155-168) for ER/PR. What do you mean by "ISH"? Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Paula Wilder" 06/02/06 4:13 PM >>> Hello Histonet! Can anyone help me with this CAP question? Our pathologist would like to know how everyone scores their ER/PRs and ISH. Any help would be greatly appreciated!!! Paula Wilder St. Joseph Medical Center Towson, MD 21204 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From portera <@t> msu.edu Fri Jun 2 15:51:11 2006 From: portera <@t> msu.edu (Amy Porter) Date: Fri Jun 2 15:49:50 2006 Subject: [Histonet] looking for a new tissue processor References: Message-ID: <000301c68686$47962640$8e7a0923@HistoJJ> We have the Thermo Electron Excelsior in our laboratory and we love it. I would do a demo if possible. ----- Original Message ----- From: "Reuel Cornelia" To: Sent: Friday, June 02, 2006 1:49 PM Subject: [Histonet] looking for a new tissue processor >I just wanted to hear from anyone what tissue processor would you like > to recommend to change our Shandon hypercenter XP. We have our machine > for 12 years now and it is still running smoothly without any > problem.Shandon Service contract will discontinue to service this > machine which has been running for 7 years although we found contractors > who are still willing to maintain our machine. I just wanted a feedback > and I will look into it. Thank you. What can one mind do without > histonet? > > ******************************************************************************************************************* > Texas Scottish Rite Hospital for Children is one of the nation's leading > pediatric centers for the > treatment of orthopedic conditions and learning disorders, like dyslexia. > This email transmission > and/or its attachments may contain confidential health information, > intended only for the use of the individual or entity named above. > > The authorized recipient of this information is prohibited from disclosing > it to any other party unless > required to do so by law and is required to delete/destroy the information > after its stated need has > been fulfilled. If you are not the intended recipient, any disclosure, > copying, distribution or action > taken in reliance on the contents of this email transmission is > prohibited. If you have received this > information in error, please notify the sender immediately and delete this > information. > > We appreciate your efforts to protect the children's confidential > information. > ******************************************************************************************************************* > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From djamesnz <@t> orcon.net.nz Fri Jun 2 15:53:21 2006 From: djamesnz <@t> orcon.net.nz (Darren James) Date: Fri Jun 2 15:53:55 2006 Subject: [Histonet] Sakura coverslipper Model 6400 In-Reply-To: Message-ID: <007601c68686$9bf1c2d0$0301010a@DAZZA> Hi there, I have used both tape and glass coverslippers extensively and while the tape coverslippers are very fast they do have their drawbacks. I accumulated quite a collection of slides over a period of time, most were coverslipped with tape. When I opened the slide box a few months ago I had a great collection of blank slides along with another collection of tape complete with sections. Apparently the tissue has a higher affinity for the tape than glass. I have heard that a second generation of tape has been released which largely resolves this but I have not had experience with this. In my experience with glass coverslippers (of many brands and models) I have encountered good and bad. They can break down frequently but other times they run for weeks with no problem. I have seen a great little glass coverslipper which is reported to be the fastest on the market. It has an incredibly small footprint and completes the whole coverslipping process in 2 actions. It is made by some guys in Norway (I think). Another thing I didn't like about tape was the continual variation of the surface i.e. it is never totally flat, unlike glass. The pathologists didn't seem to mind though and they were the ones reporting. Removing the tape is also a bit of an art form. If you just leave it to soak you can end up with a sticky mess. If you time it right however it peels right off. With glass you leave it in xylene until the coverslip falls off. Guess it's 6 of one and half a dozen of the other. Have a good one. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bauer, Karen Sent: Saturday, 3 June 2006 2:32 a.m. To: histonet@lists.utsouthwestern.edu Subject: FW: [Histonet] Sakura coverslipper Model 6400 ________________________________ From: Bauer, Karen Sent: Fri 6/2/2006 9:31 AM To: Deltour, Douglas D. (HM2) Subject: RE: [Histonet] Sakura coverslipper Model 6400 Douglas, How does the tape last over the years? Right now, we coverslip manually, but have been thinking about getting a coverslipper in the near future. I've heard mixed emotions when it comes to tape or glass coverslips. We save our slides for 20 years. Does the tape stay adhered? I remember receiving a case a while ago that had tape coverslips on them and they were all falling off. Some of the patient tissues adhered to the coverslip so it created quite a mess trying to mount the rigid tape coverslip back onto the slide. Needless to say, they were not pretty when we got them back on. Just wondering, Karen Bauer HT(ASCP) ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Deltour, Douglas D. (HM2) Sent: Fri 6/2/2006 7:44 AM To: 'Angela Bitting'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Sakura coverslipper Model 6400 I will NEVER go back to glass. Our Sakura tissue-tek SCA coverslippers are workhorses. They have not needed service in over three years. The previous supervisor ordered a Tissue-Tek Glass coverslipper and it is collecting dust. It is not as fast as the tape and the techs feel the need to watch it as it coverslipps because they do not trust it. There are many myths and reasons that people do not like tape coverslippers. I will let the others get into those reasons but for my ulcer I would not have anything else but the tape. Good luck! Douglas Deltour HT(ASCP) -----Original Message----- From: Angela Bitting [mailto:akbitting@geisinger.edu] Sent: Friday, June 02, 2006 8:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sakura coverslipper Model 6400 Hi Histonetters, I'm demoing this automated glass coverslipper for two weeks. The person who currently repairs (frequently) my tape coverslippers said "if I think I have problems now, moving to a glass coverslipper will be even worse". What are your opinions? Thanks, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jxccny <@t> yahoo.com Fri Jun 2 20:04:38 2006 From: jxccny <@t> yahoo.com (x j) Date: Fri Jun 2 20:04:41 2006 Subject: [Histonet] Anti-Glutamate Decarboxylase 65 & 67 (GAD 65/67) exp. In-Reply-To: <20060531181246.97679.qmail@web51409.mail.yahoo.com> Message-ID: <20060603010438.91623.qmail@web51411.mail.yahoo.com> Who has the experiences on Anti-Glutamate Decarboxylase 65 & 67 (GAD 65/67) experiments? We use Anti-Glutamate Decarboxylase 65 & 67 (Chemcon, Cat. #: AB1511) as primary antibody, Biotin-SP-AffiniPure Goat Anti-Rabbit IgG (Jackson, Cat. #: 111-065-144) as secondary antibody. The serum is from goat. We stain mouse sections. The problem is that the experiments worked perfect every time before. But the God is making jokes with me now: none of recent experiments works. Any suggestions are highly appreciated. __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From H.I.Grabsch <@t> leeds.ac.uk Sun Jun 4 05:48:54 2006 From: H.I.Grabsch <@t> leeds.ac.uk (Heike Grabsch) Date: Sun Jun 4 05:49:03 2006 Subject: [Histonet] (no subject) Message-ID: Dear Tim, ScyTek in the US do sell bulk reagents for immunohisotchemsitry which are more or less ready to use. On the other hand you may consider going back to the 'good old days' where we used to make up the DAB from either from actual DAB powder or tablets. Heike Dr Heike Grabsch, MRCPath MD Gastrointestinal Research Group Pathology and Tumour Biology Leeds Institute of Molecular Medicine St James's University Hospital JIF Building, Level 4 Beckett Street Leeds LS9 7TF Date: Fri, 2 Jun 2006 12:22:45 -0500 From: "Quinn, Tim L." Subject: [Histonet] Bulk DAB To: Message-ID: Content-Type: text/plain; charset="us-ascii" Dear Listies, I would like to start using 250 mL tubs for DAB staining to get staining time standardized. Is there a source for large purchase of reagents at an affordable price? Cheers, Timothy L. Quinn Senior Lab Technician Missouri University at Kansas City Medical School- Med Lab 2411 Holmes Kansas City, MO 64108 816-235-1904 quinntl@umkc.edu From yeepengtiang <@t> hotmail.com Sun Jun 4 10:47:07 2006 From: yeepengtiang <@t> hotmail.com (tiang yeepeng) Date: Sun Jun 4 10:47:11 2006 Subject: [Histonet] section adhesion on slides Message-ID: I coat my slides with poly-L-lysine but it seemed that I lost many cells during the washing steps in IHC. I use a centrifuge my cells onto the slides using the cytospin. Any idea or comment, Geoff? With regards, TIANG YEE PENG Department of Medical Microbiology, Faculty of Medicine, University of Malaya. _________________________________________________________________ It's the future, it's here, and it's free: Windows Live Mail beta http://www2.imagine-msn.com/minisites/mail/Default.aspx?locale=en-us From carl.hobbs <@t> kcl.ac.uk Sun Jun 4 11:37:16 2006 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Sun Jun 4 11:37:46 2006 Subject: [Histonet] re: Bulk DAB Message-ID: My recipe here: http://www.immunoportal.com/index.php Great site: not mine but I contribute. I must admit that I no longer heat before adding water to DAB. It dissolves well in RT water. Hope that's helpful From langxingpan <@t> pantomics.com Sun Jun 4 13:37:01 2006 From: langxingpan <@t> pantomics.com (Langxingpan) Date: Sun Jun 4 13:37:10 2006 Subject: [Histonet] RE: Histonet Digest, Vol 31, Issue 5 Message-ID: <000d01c68805$df1fb910$210110ac@Pantomics> Dear Tiang Yee, You can coat your slides using the following method. Slide Coating with 3 - aminopropyltriethoxysilane (APES; Sigma A-3648) 1. Soak glass slides in acetone containing 2% APES for 1 to 5 mins; 2. Wash the slides in acetone or distilled water briefly; 3. Dry the slides at 42 ?C. All the best, Langxing Pan, M.D., Ph.D. Pantomics, Inc. 457 Mariposa Street San Francisco, CA 94107 Direct: 1-415-863-2380 langxingpan@pantomics.com www.pantomics.com Message: 2 Date: Sun, 4 Jun 2006 15:47:07 +0000 From: "tiang yeepeng" Subject: Re: [Histonet] section adhesion on slides To: , Message-ID: Content-Type: text/plain; charset="iso-8859-1" I coat my slides with poly-L-lysine but it seemed that I lost many cells during the washing steps in IHC. I use a centrifuge my cells onto the slides using the cytospin. Any idea or comment, Geoff? With regards, TIANG YEE PENG Department of Medical Microbiology, Faculty of Medicine, University of Malaya. From DOBLE <@t> PARTNERS.ORG Sun Jun 4 14:23:49 2006 From: DOBLE <@t> PARTNERS.ORG (Oble, Darryl A.,M.D.) Date: Sun Jun 4 14:23:56 2006 Subject: [Histonet] autoclave vs. filter of RBC lysis buffer References: <20060604170155.5C875190029@phsmgmx3.partners.org> Message-ID: I was wondering whether anyone knew why RBC lysis buffer is always filtered and not autoclaved. Below are the ingredients. I saw some material on the internet about corrosion of the autoclave, but this was for a different NH4Cl containing solution. Thanks for any help that you can offer. 4.14g NH4Cl 0.5g KHCO3 0.018g EDTA dissolve in water adjust pH to 7.35 with 0.1NaOH Total volume 500ml Regards, Frustrated Scientist -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of histonet-request@lists.utsouthwestern.edu Sent: Sun 6/4/2006 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 31, Issue 5 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. (no subject) (Heike Grabsch) 2. Re: section adhesion on slides (tiang yeepeng) 3. re: Bulk DAB (Carl Hobbs) ---------------------------------------------------------------------- Message: 1 Date: Sun, 4 Jun 2006 11:48:54 +0100 From: "Heike Grabsch" Subject: [Histonet] (no subject) To: Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" Dear Tim, ScyTek in the US do sell bulk reagents for immunohisotchemsitry which are more or less ready to use. On the other hand you may consider going back to the 'good old days' where we used to make up the DAB from either from actual DAB powder or tablets. Heike Dr Heike Grabsch, MRCPath MD Gastrointestinal Research Group Pathology and Tumour Biology Leeds Institute of Molecular Medicine St James's University Hospital JIF Building, Level 4 Beckett Street Leeds LS9 7TF Date: Fri, 2 Jun 2006 12:22:45 -0500 From: "Quinn, Tim L." Subject: [Histonet] Bulk DAB To: Message-ID: Content-Type: text/plain; charset="us-ascii" Dear Listies, I would like to start using 250 mL tubs for DAB staining to get staining time standardized. Is there a source for large purchase of reagents at an affordable price? Cheers, Timothy L. Quinn Senior Lab Technician Missouri University at Kansas City Medical School- Med Lab 2411 Holmes Kansas City, MO 64108 816-235-1904 quinntl@umkc.edu ------------------------------ Message: 2 Date: Sun, 4 Jun 2006 15:47:07 +0000 From: "tiang yeepeng" Subject: Re: [Histonet] section adhesion on slides To: , Message-ID: Content-Type: text/plain; charset="iso-8859-1" I coat my slides with poly-L-lysine but it seemed that I lost many cells during the washing steps in IHC. I use a centrifuge my cells onto the slides using the cytospin. Any idea or comment, Geoff? With regards, TIANG YEE PENG Department of Medical Microbiology, Faculty of Medicine, University of Malaya. _________________________________________________________________ It's the future, it's here, and it's free: Windows Live Mail beta http://www2.imagine-msn.com/minisites/mail/Default.aspx?locale=en-us ------------------------------ Message: 3 Date: Sun, 4 Jun 2006 17:37:16 +0100 From: "Carl Hobbs" Subject: [Histonet] re: Bulk DAB To: "Histonet" Message-ID: Content-Type: text/plain; charset="iso-8859-1" My recipe here: http://www.immunoportal.com/index.php Great site: not mine but I contribute. I must admit that I no longer heat before adding water to DAB. It dissolves well in RT water. Hope that's helpful ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 31, Issue 5 *************************************** From langxingpan <@t> pantomics.com Sun Jun 4 16:18:11 2006 From: langxingpan <@t> pantomics.com (Langxingpan) Date: Sun Jun 4 16:18:20 2006 Subject: [Histonet] RE: Histonet Digest, Vol 31, Issue 4 Message-ID: <000001c6881c$635710f0$210110ac@Pantomics> Dear Marian, We are providing IHC tissue array controls for laboratory use. Our lymphoma panels contain tissue array controls for cyclin D1, bcl-2, bcl-6, kappa and lambda light chains. All these are currently being validated in our collaborative IHC centers. They should be ready quite soon. Please check with me in two weeks. Regards, Langxing Pan, M.D., Ph.D. Pantomics, Inc. 457 Mariposa Street San Francisco, CA 94107 Direct: 1-415-863-2380 langxingpan@pantomics.com www.pantomics.com Message: 7 Date: Fri, 2 Jun 2006 16:15:46 -0400 From: "Marian Powers" Subject: [Histonet] MCL CONTROL SLIDES To: Message-ID: <000801c68681$55d84da0$3227c847@D7XQNX91> Content-Type: text/plain; charset="iso-8859-1" Hello, looking to purchase mantle cell lymphoma control slides, any recommendations? Thanks in advance, Marian*************************************** From langxingpan <@t> pantomics.com Mon Jun 5 03:06:59 2006 From: langxingpan <@t> pantomics.com (Langxingpan) Date: Mon Jun 5 03:07:10 2006 Subject: [Histonet] RE: Histonet Digest, Vol 31, Issue 4 Message-ID: <000001c68877$061b0e40$210110ac@Pantomics> Dear Paula, You presumably mean ER/PR IHC scoring system rather than ISH. The key element in the ER IHC scoring is the percentage of the nuclear staining. Like many other labs, we set a "positive" ER result at a minimum of 5% nuclear staining. The scoring includes "-" (<5%), "+" (5%~30%), "++" (30%~80%) and "+++" (>80%). We also incorporate staining intensity as well, but this will be recorded with reference to our positive control. Our ER control is tissue array-based, which contains three cases of "-~+", "++" and "+++" ER expressers. We produce these and other IHC control arrays. If you want to have a try, I can send you some of the ER test control Other people may use 10% of nuclear staining as the threshold for ER positivity. However, NIH recommends that any positive nuclear ER staining is considered as a positive result. Regards, Langxing Pan, M.D., Ph.D. Pantomics, Inc. 457 Mariposa Street San Francisco, CA 94107 Direct: 415-863-2380 Fax: 510-653-1227 langxingpan@pantomics.com www.pantomics.com Message: 9 Date: Fri, 02 Jun 2006 16:40:55 -0400 From: "Richard Cartun" Subject: Re: [Histonet] Scoring of ER/PR and ISH To: "Paula Wilder" , Message-ID: <44806A1702000077000004CD@hcnwgwds01.hh.chs> Content-Type: text/plain; charset=US-ASCII We use Dr. Craig Allred's scoring system (see Mod Pathol 1998;11(2):155-168) for ER/PR. What do you mean by "ISH"? Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Paula Wilder" 06/02/06 4:13 PM >>> Hello Histonet! Can anyone help me with this CAP question? Our pathologist would like to know how everyone scores their ER/PRs and ISH. Any help would be greatly appreciated!!! Paula Wilder St. Joseph Medical Center Towson, MD 21204 From timo.vaisanen <@t> oulu.fi Mon Jun 5 08:53:32 2006 From: timo.vaisanen <@t> oulu.fi (Timo =?iso-8859-1?b?VuRpc+RuZW4=?=) Date: Mon Jun 5 08:53:36 2006 Subject: [Histonet] (no subject) Message-ID: <1149515612.4484375ca04cc@webmail.oulu.fi> Dear all, Has anyone encountered the following problem? The surface of newly embedded paraffin blocks start to become concave only after few days. This is not a problem with normal tissue specimens but with for example small biopsies the uneveness creates difficulties. Biopsies on the edge of the block run out if you try to trim the block enough to get the samples in the middle of the block to the same section with the other samples. I quess paraffin can contract but I have always thought that this takes years. Could there be something wrong with the paraffin? Any suggestions? Thank's in advance, Timo Timo V?is?nen PhD Oulu University Hospital Department of Pathology Finland From JWEEMS <@t> sjha.org Mon Jun 5 08:58:22 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Mon Jun 5 08:57:41 2006 Subject: [Histonet] (no subject) Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD320295FD83@sjhaexc02.sjha.org> This sounds like your tissue is not well dehydrated and cleared. When those steps are inadequate, the paraffin cannot impregnate the tissue adequately. The alcohol and xylene then evaporate, leaving the sinkhole in the tissue. My 2 cents worth, j:>) Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Timo V?is?nen Sent: Monday, June 05, 2006 9:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Dear all, Has anyone encountered the following problem? The surface of newly embedded paraffin blocks start to become concave only after few days. This is not a problem with normal tissue specimens but with for example small biopsies the uneveness creates difficulties. Biopsies on the edge of the block run out if you try to trim the block enough to get the samples in the middle of the block to the same section with the other samples. I quess paraffin can contract but I have always thought that this takes years. Could there be something wrong with the paraffin? Any suggestions? Thank's in advance, Timo Timo V?is?nen PhD Oulu University Hospital Department of Pathology Finland _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From hej01 <@t> health.state.ny.us Mon Jun 5 09:06:36 2006 From: hej01 <@t> health.state.ny.us (Helen E Johnson) Date: Mon Jun 5 09:06:49 2006 Subject: [Histonet] IHC in Free-Floating Sections Message-ID: Hi Histonetters, Immunohistochemistry will be performed using free-floating tissue sections. How will the sections be mounted on slides and coverslipped? Will special slides and mounting media need to be used? Helen Johnson (hej01@health.state.ny.us) From Eric.C.Kellar <@t> questdiagnostics.com Mon Jun 5 09:58:09 2006 From: Eric.C.Kellar <@t> questdiagnostics.com (Kellar, Eric C) Date: Mon Jun 5 09:58:51 2006 Subject: [Histonet] (no subject) Message-ID: <6843061CE6B98E4B96590D4F299618F801583BC1@qdcws0117.us.qdx.com> Timo, I agree with Joyce! Your infiltrating paraffins could also be contaminated with a carry over of xylene during processing. If your final paraffins are not changed frequently enough, too much residual xylene deposits into the paraffins (you can usually smell it). This can result in sinkholes and an uneven block face, as the xylene evaporates out. Eric C. Kellar Quest Diagnostics, Inc Miami -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Weems, Joyce Sent: Monday, June 05, 2006 9:58 AM To: Timo V?is?nen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (no subject) This sounds like your tissue is not well dehydrated and cleared. When those steps are inadequate, the paraffin cannot impregnate the tissue adequately. The alcohol and xylene then evaporate, leaving the sinkhole in the tissue. My 2 cents worth, j:>) Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Timo V?is?nen Sent: Monday, June 05, 2006 9:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Dear all, Has anyone encountered the following problem? The surface of newly embedded paraffin blocks start to become concave only after few days. This is not a problem with normal tissue specimens but with for example small biopsies the uneveness creates difficulties. Biopsies on the edge of the block run out if you try to trim the block enough to get the samples in the middle of the block to the same section with the other samples. I quess paraffin can contract but I have always thought that this takes years. Could there be something wrong with the paraffin? Any suggestions? Thank's in advance, Timo Timo V?is?nen PhD Oulu University Hospital Department of Pathology Finland _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. ============================================================================== The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. ============================================================================== From Olivier.Leroux <@t> UGent.be Mon Jun 5 13:34:27 2006 From: Olivier.Leroux <@t> UGent.be (Olivier Leroux) Date: Mon Jun 5 13:34:41 2006 Subject: [Histonet] coatings for slides Message-ID: <1149532467.44847933d7572@mail.ugent.be> Best, Thank you for all the reactions regarding slidecoatings! regards, Olivier -- Olivier Leroux Ghent University Department of Biology - Pteridological Section K.L. Ledeganckstraat 35 B-9000 Belgium http://www.pteridology.ugent.be Olivier.Leroux@UGent.be From Steven2146 <@t> aol.com Mon Jun 5 13:46:15 2006 From: Steven2146 <@t> aol.com (Steven2146@aol.com) Date: Mon Jun 5 13:46:30 2006 Subject: [Histonet] bone decalcification Message-ID: <4b6.12a3da9.31b5d5f7@aol.com> Hello... I was wondering if anyone has a procedure for a rapid bone decalcification prior to frozen sectioning? Thanks Steven From rjbuesa <@t> yahoo.com Mon Jun 5 14:56:49 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jun 5 14:56:54 2006 Subject: [Histonet] (no subject) In-Reply-To: <1149515612.4484375ca04cc@webmail.oulu.fi> Message-ID: <20060605195649.14500.qmail@web61212.mail.yahoo.com> Timo: Which is your processing protocol? How frequently do you change your reagents? I have been told that in your country xylene is being susbtituted by isopropanol. Are you using a xylene substitute? It seems that the tissues are reaching the paraffin with a large "carry over" from the previous reagents and the problem you describe was quite frequent in the past when aniline oil and benzene were carried over. On such cases the paraffin around the tissue not only contracted but became darker and softer. Try to check the changing schedule on your reagents; probably that will be the cause. Hope this will help you. Ren? J. Timo V?is?nen wrote: Dear all, Has anyone encountered the following problem? The surface of newly embedded paraffin blocks start to become concave only after few days. This is not a problem with normal tissue specimens but with for example small biopsies the uneveness creates difficulties. Biopsies on the edge of the block run out if you try to trim the block enough to get the samples in the middle of the block to the same section with the other samples. I quess paraffin can contract but I have always thought that this takes years. Could there be something wrong with the paraffin? Any suggestions? Thank's in advance, Timo Timo V?is?nen PhD Oulu University Hospital Department of Pathology Finland _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. From lu_ze <@t> sbcglobal.net Mon Jun 5 15:02:50 2006 From: lu_ze <@t> sbcglobal.net (Ze Lu) Date: Mon Jun 5 15:02:56 2006 Subject: [Histonet] Anti roll plate on IEC plus cryotome, need help Message-ID: <02a701c688db$05e7cad0$0602a8c0@OPTIMUM2> Hi, histonet friends, We have an old IEC plus cryotome. We changed to a disposable blade system. According to the IEC plus mannual, we adjust the anti roll plate. However, we tried couple sectioning, the section always curved and get folded. I used different crytome, for all of them, there is only small angle (or space) between blade holder and anti roll plate where secion go through, that section can not be folded after leave the block. However for IEC plus, it really has big angle according to their description. I think someone know what I am talking about if he used this model. Can someone share his experience on this? Also, we are going to change the anti roll plate, can someone kindly let us know which product will fit to this model. We appreciate very much. ===================== Ze Lu, Ph.D., Optimum Therapeutics, LLC From pmarcum <@t> vet.upenn.edu Mon Jun 5 16:10:54 2006 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Mon Jun 5 16:11:05 2006 Subject: [Histonet] Pennsylvania Delegates for teh NSH Meeting in Phoenix Message-ID: <6.1.1.1.2.20060605170714.0197a618@mail.vet.upenn.edu> Good Afternoon All PA NSH Members and Region 2, If you are going to the NSH meeting and can act as a delegate to the House of Delegates for PA please notify either myself or Gloria Limetti as soon as possible. The HOD meets in Wednesday evening at 7PM. You can reach Gloria at glorialimetti@yahoo.com Thanks and have a great meeting! Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From gcallis <@t> montana.edu Mon Jun 5 16:39:21 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Jun 5 16:39:29 2006 Subject: [Histonet] Anti roll plate on IEC plus cryotome, need help In-Reply-To: <02a701c688db$05e7cad0$0602a8c0@OPTIMUM2> References: <02a701c688db$05e7cad0$0602a8c0@OPTIMUM2> Message-ID: <6.0.0.22.1.20060605152034.01b3afe0@gemini.msu.montana.edu> The antiroll plate for this old IEC was probably developed for use with c profile steel knives. I suggest you take the plate off and learn to do frozen sections using a sable brush, or some type of brush that works best for you. A very nice tutorial on how to use brush technic is found at www.pathologyinnovations.com Talk to a technical services person at Triangle Biomedical Systems (?) (TBS) about a different or workable antiroll plate or before you spend the money on a device that will be useless. TBS has a website and they sell IEC's. Personally, we prefer no antiroll plate and do brush technic for 99% of our cryostat work - it is faster, no readjustment of plates, etc. At 02:02 PM 6/5/2006, you wrote: >Hi, histonet friends, > >We have an old IEC plus cryotome. We changed to a disposable blade system. >According to the IEC plus mannual, we adjust the anti roll plate. However, >we tried couple sectioning, the section always curved and get folded. I >used different crytome, for all of them, there is only small angle (or >space) between blade holder and anti roll plate where secion go through, >that section can not be folded after leave the block. However for IEC >plus, it really has big angle according to their description. I think >someone know what I am talking about if he used this model. Can someone >share his experience on this? Also, we are going to change the anti roll >plate, can someone kindly let us know which product will fit to this >model. We appreciate very much. > > > >===================== >Ze Lu, Ph.D., >Optimum Therapeutics, LLC > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From Barry.R.Rittman <@t> uth.tmc.edu Mon Jun 5 16:47:54 2006 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Mon Jun 5 16:47:59 2006 Subject: [Histonet] Anti roll plate on IEC plus cryotome, need help Message-ID: I have not used an IEC for some time but there were always problems with the anti roll plate. We ended up cutting glass ones of our own (from a 3 x 2" glass slide), adding a thin strip of sellotape along each side to provide clearance for the section and then spraying the surface with a Teflon spray to prevent sections sticking. These plates were significantly better than the plastic anti roll plates. We never had much luck getting good serial sections of skin using the brush technique but as Gayle says whatever works for you. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Monday, June 05, 2006 4:39 PM To: Ze Lu; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Anti roll plate on IEC plus cryotome, need help The antiroll plate for this old IEC was probably developed for use with c profile steel knives. I suggest you take the plate off and learn to do frozen sections using a sable brush, or some type of brush that works best for you. A very nice tutorial on how to use brush technic is found at www.pathologyinnovations.com Talk to a technical services person at Triangle Biomedical Systems (?) (TBS) about a different or workable antiroll plate or before you spend the money on a device that will be useless. TBS has a website and they sell IEC's. Personally, we prefer no antiroll plate and do brush technic for 99% of our cryostat work - it is faster, no readjustment of plates, etc. At 02:02 PM 6/5/2006, you wrote: >Hi, histonet friends, > >We have an old IEC plus cryotome. We changed to a disposable blade system. >According to the IEC plus mannual, we adjust the anti roll plate. However, >we tried couple sectioning, the section always curved and get folded. I >used different crytome, for all of them, there is only small angle (or >space) between blade holder and anti roll plate where secion go through, >that section can not be folded after leave the block. However for IEC >plus, it really has big angle according to their description. I think >someone know what I am talking about if he used this model. Can someone >share his experience on this? Also, we are going to change the anti roll >plate, can someone kindly let us know which product will fit to this >model. We appreciate very much. > > > >===================== >Ze Lu, Ph.D., >Optimum Therapeutics, LLC > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From omnivore98 <@t> yahoo.com Mon Jun 5 21:22:24 2006 From: omnivore98 <@t> yahoo.com (Heather Renko) Date: Mon Jun 5 21:22:30 2006 Subject: [Histonet] Automation Ventana vs. Dako Message-ID: <20060606022224.48239.qmail@web31304.mail.mud.yahoo.com> As an ex Ventana Tech Rep (not sales) I can say that you really have to judge what instrument is best suited for your volume and your staffing. Ventana is a great company with a great product but, very expensive. Can you rely on it-yes, Can any tech run it with minimal training-yes. Have they had lot issues with detection, sure but name a company that hasn't run into that at some point. Dako on the other hand is very reliable and has the market on good antibodies. Can anyone run it-not without a bit of hands training. It seems to be more affordable than Ventana but, you have to have a good staff that can consistently reproduce steps and make up dilutions. With Ventana you can cut, dry, put it on the instrument and then walk away-reproducible and consistent pretty much every time. With Ventana you can also create an open system and run some great Dako antibodies or any other vendors products for that fact. Your only marrried to their detection. Both companies seem to have good tech support and Dako has allot of really sharp publications to get you off and running. Unfortunately in today's histology labs we are seeing such an increase in staffing changes that it is sometimes better to pay the extra money and let the Ventana Benchmark do the work and not worry about it. On the other hand if you have a good tech staff that can be "cooks in the kitchen" than Dako seems to be a pretty good instrument too. I welcome you to listen to your peers-its just simply finding out which company will suit your needs and what your budget will endure. Just my un-bias two cents. I wish you all the best in your decison making process. Heather __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From AnthonyH <@t> chw.edu.au Mon Jun 5 22:15:29 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Jun 5 22:15:38 2006 Subject: [Histonet] Concave wax blocks Message-ID: Timo, The Concave wax blocks problem occurs when there is a little too much xylene left in the wax blocks or if the blocks are insufficiently processed. Check the age of the last wax (how many blocks have been processed) or you might need to increase the time of processing. Is the vacuum still working? What seems to happen is that as the wax block dries, xylene &/or alcohol evaporates causing concavity of the blocks. To fix it you will need to melt the blocks and re-embed them in fresh wax (to obtain quick sections). For long term correction, leave the blocks in molten wax to fully remove any trace solvents and re-embed. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Timo V?is?nen Sent: Monday, 5 June 2006 11:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Dear all, Has anyone encountered the following problem? The surface of newly embedded paraffin blocks start to become concave only after few days. This is not a problem with normal tissue specimens but with for example small biopsies the uneveness creates difficulties. Biopsies on the edge of the block run out if you try to trim the block enough to get the samples in the middle of the block to the same section with the other samples. I quess paraffin can contract but I have always thought that this takes years. Could there be something wrong with the paraffin? Any suggestions? Thank's in advance, Timo Timo V?is?nen PhD Oulu University Hospital Department of Pathology Finland _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From kemlo.rogerson <@t> waht.swest.nhs.uk Tue Jun 6 02:11:52 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Jun 6 02:11:24 2006 Subject: [Histonet] bone decalcification Message-ID: Make thin slices of bone and suspend in 10% soln of EDTA in 0.1M Tris buffer pH 6.95 (add KOH pellets to achieve that). Keep soln in cold room (4 degrees C) and stir with magnetic stirrer. Add bone which ought to decalcify within 24 hours. Freeze slices mount on chuck and cut sections; the knife may have to be cooled with a block of CO2 ice or to cool the microtome. Courtesy of the great God R.D. Lillie. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Not all that is gold glitters, not all who wander are lost. --Tolkein This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From abright <@t> brightinstruments.com Tue Jun 6 04:40:19 2006 From: abright <@t> brightinstruments.com (Alan Bright) Date: Tue Jun 6 04:29:28 2006 Subject: [Histonet] Anti roll plate on IEC plus cryotome, need help Message-ID: Dear Ze, The MagnaCut Anti-Roll Plate ( Part No: 50300-1) pack of 3 with handle, is an ideal product for your problem. This anti-roll plate is simply placed onto the knife or blade and self adjusts for instant flat sections they are inexpensive and fool proof. Need I say anymore. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype User ID: dazzle0 -----Original Message----- From: Ze Lu [mailto:lu_ze@sbcglobal.net] Sent: 05 June 2006 21:03 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Anti roll plate on IEC plus cryotome, need help Hi, histonet friends, We have an old IEC plus cryotome. We changed to a disposable blade system. According to the IEC plus mannual, we adjust the anti roll plate. However, we tried couple sectioning, the section always curved and get folded. I used different crytome, for all of them, there is only small angle (or space) between blade holder and anti roll plate where secion go through, that section can not be folded after leave the block. However for IEC plus, it really has big angle according to their description. I think someone know what I am talking about if he used this model. Can someone share his experience on this? Also, we are going to change the anti roll plate, can someone kindly let us know which product will fit to this model. We appreciate very much. ===================== Ze Lu, Ph.D., Optimum Therapeutics, LLC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Diane.Gladney <@t> se.amedd.army.mil Tue Jun 6 05:35:12 2006 From: Diane.Gladney <@t> se.amedd.army.mil (Gladney, Diane C Ms MACH) Date: Tue Jun 6 05:35:49 2006 Subject: [Histonet] looking for a new tissue processor In-Reply-To: Message-ID: <4D55B2E997EFAE4DA6081DDE100B8302E714D2@amedmlsermc133> We bought a Leica ASP 300 last May and have purchased another one to replace our 25+ year old VIP. This processor is very user friendly and has so many features that are a histotech's dream come true. The service personnel are top notch and have always given me immediate help. I have had few problems with this processor and what problems I did have were corrected almost immediately. I was assured that if they could not fix the problem to my satisfaction that I would be given a new processor to replace it. These processors are assembled in Germany then shipped to the USA. So, anything can happen in shipment to damage the processor. Included in the cost of the processor is a trip to Chicago to receive training on the equipment. This training is very useful. The staff in Chicago are very knowledgeable and are always available to answer any questions when you return to work. I have an outstanding Sales Representative that has helped me tremendously. She stays in contact with me even after the sale. I can call on her anytime if I am having problems and she will get me the help that I need. I know that maybe all Sales Representatives are not as diligent as she is, but she is a credit to the caliber of employees that work for Leica. Thanks, Diane Diane C. Gladney, HT (ASCP) Supervisor, Histology Dept. Moncrief Army Community Hospital Dept. of Pathology 4500 Stuart St. FT. Jackson, SC 29207 Email: diane.gladney@se.amedd.army.mil Phone: 803-751-2530 FAX: 803-751-7829 DSN: 734-2530 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Reuel Cornelia Sent: Friday, June 02, 2006 1:50 PM To: histonet@pathology.swmed.edu Subject: [Histonet] looking for a new tissue processor I just wanted to hear from anyone what tissue processor would you like to recommend to change our Shandon hypercenter XP. We have our machine for 12 years now and it is still running smoothly without any problem.Shandon Service contract will discontinue to service this machine which has been running for 7 years although we found contractors who are still willing to maintain our machine. I just wanted a feedback and I will look into it. Thank you. What can one mind do without histonet? ************************************************************************ ******************************************* Texas Scottish Rite Hospital for Children is one of the nation's leading pediatric centers for the treatment of orthopedic conditions and learning disorders, like dyslexia. This email transmission and/or its attachments may contain confidential health information, intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing it to any other party unless required to do so by law and is required to delete/destroy the information after its stated need has been fulfilled. If you are not the intended recipient, any disclosure, copying, distribution or action taken in reliance on the contents of this email transmission is prohibited. If you have received this information in error, please notify the sender immediately and delete this information. We appreciate your efforts to protect the children's confidential information. ************************************************************************ ******************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cfrenchko <@t> cytomyx.com Tue Jun 6 06:53:04 2006 From: cfrenchko <@t> cytomyx.com (Carla Frenchko) Date: Tue Jun 6 06:50:05 2006 Subject: [Histonet] Automation Ventana vs. Dako Message-ID: <47715D62D777924289EA32B06544309E0301CA@cytom-us-01.cytomyx.com.local> With all due respect to Heather; but the reason the Ventana is not really an "open" system is that you are "married" to a particular vendor's antibodies and detection. In a true "open" system most any company's antibodies will run with the instrument's detection (specifically speaking of both the Dako and Biogenex instruments having run both). Sincerely, Carla -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heather Renko Sent: Monday, June 05, 2006 10:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automation Ventana vs. Dako As an ex Ventana Tech Rep (not sales) I can say that you really have to judge what instrument is best suited for your volume and your staffing. Ventana is a great company with a great product but, very expensive. Can you rely on it-yes, Can any tech run it with minimal training-yes. Have they had lot issues with detection, sure but name a company that hasn't run into that at some point. Dako on the other hand is very reliable and has the market on good antibodies. Can anyone run it-not without a bit of hands training. It seems to be more affordable than Ventana but, you have to have a good staff that can consistently reproduce steps and make up dilutions. With Ventana you can cut, dry, put it on the instrument and then walk away-reproducible and consistent pretty much every time. With Ventana you can also create an open system and run some great Dako antibodies or any other vendors products for that fact. Your only marrried to their detection. Both companies seem to have good tech support and Dako has allot of really sharp publications to get you off and running. Unfortunately in today's histology labs we are seeing such an increase in staffing changes that it is sometimes better to pay the extra money and let the Ventana Benchmark do the work and not worry about it. On the other hand if you have a good tech staff that can be "cooks in the kitchen" than Dako seems to be a pretty good instrument too. I welcome you to listen to your peers-its just simply finding out which company will suit your needs and what your budget will endure. Just my un-bias two cents. I wish you all the best in your decison making process. Heather __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From la.sebree <@t> hosp.wisc.edu Tue Jun 6 07:52:30 2006 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Tue Jun 6 07:52:37 2006 Subject: [Histonet] Automation Ventana vs. Dako Message-ID: Misconception here: you can run antibodies from any company on Ventana instruments either by putting them in a user-fillable Ventana dispenser or by manually applying the antibody in a titration mode run. We do both of these as well as use some of Ventana's antibodies. The only thing you are "married to" is their detection chemistries, biotin block (if using) and amplification (if using). You can employ your own enzyme digestion reagent and counterstain if desired. If performing antigen retrieval on line, you also are wed to Ventana's Cell Conditioning solutions (HIER buffers)....although nothing prevents you from using your own HIER methods and reagents off-line which is what we do when using our NexES instrument. Just trying to clear a few things up. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carla Frenchko Sent: Tuesday, June 06, 2006 6:53 AM To: Heather Renko; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Automation Ventana vs. Dako With all due respect to Heather; but the reason the Ventana is not really an "open" system is that you are "married" to a particular vendor's antibodies and detection. In a true "open" system most any company's antibodies will run with the instrument's detection (specifically speaking of both the Dako and Biogenex instruments having run both). Sincerely, Carla -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heather Renko Sent: Monday, June 05, 2006 10:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automation Ventana vs. Dako As an ex Ventana Tech Rep (not sales) I can say that you really have to judge what instrument is best suited for your volume and your staffing. Ventana is a great company with a great product but, very expensive. Can you rely on it-yes, Can any tech run it with minimal training-yes. Have they had lot issues with detection, sure but name a company that hasn't run into that at some point. Dako on the other hand is very reliable and has the market on good antibodies. Can anyone run it-not without a bit of hands training. It seems to be more affordable than Ventana but, you have to have a good staff that can consistently reproduce steps and make up dilutions. With Ventana you can cut, dry, put it on the instrument and then walk away-reproducible and consistent pretty much every time. With Ventana you can also create an open system and run some great Dako antibodies or any other vendors products for that fact. Your only marrried to their detection. Both companies seem to have good tech support and Dako has allot of really sharp publications to get you off and running. Unfortunately in today's histology labs we are seeing such an increase in staffing changes that it is sometimes better to pay the extra money and let the Ventana Benchmark do the work and not worry about it. On the other hand if you have a good tech staff that can be "cooks in the kitchen" than Dako seems to be a pretty good instrument too. I welcome you to listen to your peers-its just simply finding out which company will suit your needs and what your budget will endure. Just my un-bias two cents. I wish you all the best in your decison making process. Heather __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cfrenchko <@t> cytomyx.com Tue Jun 6 08:32:18 2006 From: cfrenchko <@t> cytomyx.com (Carla Frenchko) Date: Tue Jun 6 08:29:15 2006 Subject: [Histonet] Automation Ventana vs. Dako Message-ID: <47715D62D777924289EA32B06544309E0301CD@cytom-us-01.cytomyx.com.local> Linda you write much more eloquently than I. What I was trying to convey is that in an open system any antibody or reagent can be used on the system, including detection. Carla -----Original Message----- From: Sebree Linda A. [mailto:la.sebree@hosp.wisc.edu] Sent: Tuesday, June 06, 2006 8:53 AM To: Carla Frenchko; Heather Renko; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Automation Ventana vs. Dako Misconception here: you can run antibodies from any company on Ventana instruments either by putting them in a user-fillable Ventana dispenser or by manually applying the antibody in a titration mode run. We do both of these as well as use some of Ventana's antibodies. The only thing you are "married to" is their detection chemistries, biotin block (if using) and amplification (if using). You can employ your own enzyme digestion reagent and counterstain if desired. If performing antigen retrieval on line, you also are wed to Ventana's Cell Conditioning solutions (HIER buffers)....although nothing prevents you from using your own HIER methods and reagents off-line which is what we do when using our NexES instrument. Just trying to clear a few things up. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carla Frenchko Sent: Tuesday, June 06, 2006 6:53 AM To: Heather Renko; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Automation Ventana vs. Dako With all due respect to Heather; but the reason the Ventana is not really an "open" system is that you are "married" to a particular vendor's antibodies and detection. In a true "open" system most any company's antibodies will run with the instrument's detection (specifically speaking of both the Dako and Biogenex instruments having run both). Sincerely, Carla -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heather Renko Sent: Monday, June 05, 2006 10:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automation Ventana vs. Dako As an ex Ventana Tech Rep (not sales) I can say that you really have to judge what instrument is best suited for your volume and your staffing. Ventana is a great company with a great product but, very expensive. Can you rely on it-yes, Can any tech run it with minimal training-yes. Have they had lot issues with detection, sure but name a company that hasn't run into that at some point. Dako on the other hand is very reliable and has the market on good antibodies. Can anyone run it-not without a bit of hands training. It seems to be more affordable than Ventana but, you have to have a good staff that can consistently reproduce steps and make up dilutions. With Ventana you can cut, dry, put it on the instrument and then walk away-reproducible and consistent pretty much every time. With Ventana you can also create an open system and run some great Dako antibodies or any other vendors products for that fact. Your only marrried to their detection. Both companies seem to have good tech support and Dako has allot of really sharp publications to get you off and running. Unfortunately in today's histology labs we are seeing such an increase in staffing changes that it is sometimes better to pay the extra money and let the Ventana Benchmark do the work and not worry about it. On the other hand if you have a good tech staff that can be "cooks in the kitchen" than Dako seems to be a pretty good instrument too. I welcome you to listen to your peers-its just simply finding out which company will suit your needs and what your budget will endure. Just my un-bias two cents. I wish you all the best in your decison making process. Heather __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From John.Thweatt <@t> va.gov Tue Jun 6 08:46:19 2006 From: John.Thweatt <@t> va.gov (Thweatt, John T) Date: Tue Jun 6 08:46:26 2006 Subject: [Histonet] Farewell to the VA, Texas (and the histonet for now) Message-ID: <631842D8371C4143A8C422186903F54628E9D3@VHAV18MSGA1.v18.med.va.gov> Dear All, I will soon be leaving the VA (U.S. Department of Veteran's Affairs) Hospital after much painstaking thought and deliberation. It may seem a little sudden, but it was a decision that has been brewing for months. It is hard to depart and walk away after 16 years with the VA, 14 at this VA in Amarillo, but in order to pursue one opportunity, you have to leave and let another go. Unfortunately you cannot have your cake and eat it too, that is what makes it so hard. I am leaving my home state of Texas and seeking work and adventure in the great state of Washington. It is sad and difficult, but I must say goodbye now to all my good friends and fellow histotechs from the great state of Texas. I can only hope now that the histology community would take me in and accept my company (and employment) in my new home state of Washington! I think back and reflect on what makes the histonet so unique and great and what immediately comes to mind is the great bond of people out there in the Histology community that are willing to reach out and help others along the way in their career, just as you have helped in mine. It is as if the "Histonet" takes on an identity of its own, a dynamic "histoforce" if you may. If there was ever a question or a concern in this growing field of ours, one just had to post and wait just a few hours before a whole host of replies would appear from all over our nation, and now all over the world as well. What a great system! Who ever created it is nothing short of genius! And let us not forget the hosting sponsors. Without them, this idea or concept would not be possible. The multipurpose index-able database would also fail to exist. If you were ever interested in a certain topic, stay tuned as the posts appear, there is something out there for everyone, beginner or novice. I have really benefited from such a wealth of information from such a seasoned pool of experts, and I am also not afraid to return the favor should a question arise that I can help someone with. I always enjoy reading the posts from the regulars on the "net" and hope to be reunited with you all in the very near future. A million thoughts are going through my mind at this time. Certain key words and phrases are buzzing through my head to the point of distraction. I feel obligated to say something cleaver, or witty or even somewhat wise, so that you all would remember me by, but I am asking the impossible as I know that people do not want to hear all this; the bottom line is I am absolutely awful when it comes to say goodbye. I prefer to say so-long for now as I intend to return to the histonet soon once I get moved, settled and back to work in Washington. Sometimes you have to be willing to take on some risks, to go forward and step out there and make this big change of location and scenery to the Pacific Northwest, and this again is part of it. Vision without action, is only a dream. That is why we must go to pursue that dream and that also means having no regrets in doing it. My last day with the VA in the Histology lab is this coming Friday, June 9th It has been fun. For now you will all be missed. ************************************ Tom Thweatt, HT (ASCP) Amarillo VA Health Care System Pathology and Laboratory Medicine Service Anatomical Pathology 6010 Amarillo Boulevard West Amarillo, TX 79106 806-355-9703 x 7071 fax 806-354-7865 From sae2001 <@t> med.cornell.edu Tue Jun 6 09:39:30 2006 From: sae2001 <@t> med.cornell.edu (Scott A. Ely) Date: Tue Jun 6 09:40:00 2006 Subject: [Histonet] Dako Eridan Message-ID: <8addb144df2.44855b62@med.cornell.edu> Do any of you use the Dako Eridan IHC stainer? What do you think of it? Scott Ely, MD MPH Section of Hematopathology Department of Pathology Weill Medical College of Cornell University New York Presbyterian Hospital 525 E. 68th Street New York, NY 10021 PH: 212-746-2442 Legal Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the original intended recipient(s) selected by Dr. Ely and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the recipient specified by Dr. Ely, please contact the sender by reply e-mail and destroy all copies of the original message. From Wanda.Smith <@t> HCAhealthcare.com Tue Jun 6 10:06:29 2006 From: Wanda.Smith <@t> HCAhealthcare.com (Smith Wanda) Date: Tue Jun 6 10:06:34 2006 Subject: [Histonet] Yellow Walls in the Lab Message-ID: Good Morning Netters, As much as I hate to admit this...the walls in my lab where the tissue processors, embedding center, UPS for the embedding center, and microtomes are, they are turning yellow. It has been happening gradually, however now when the histotechs wipe the counters each day, the paper towel is yellow. It almost looks like nicotine tar, but they assure me they are not smoking in there in the mornings!!! My lab is 3 years old and they designed it with adequate ventilation to remove fumes, but I'm at a loss on this one. Please help!!!!! Wanda From stern <@t> ipmc.cnrs.fr Tue Jun 6 10:21:17 2006 From: stern <@t> ipmc.cnrs.fr (Alejandro Ortiz Stern) Date: Tue Jun 6 10:17:17 2006 Subject: [Histonet] Help with Lung architecture Message-ID: <44859D6D.7060109@ipmc.cnrs.fr> Hi, I am currently searching for a protocol to maintain Lung structure intact while avoiding intra tracheal instillation of liquids since I am very interested in cells that lie in the outer-most layers of the lung (cells that would normally become detached upon lung lavage) Or if such cells remain attached during paraformaldehyde instillation or OCT instillation that would also be very valuable information (I have no idea). If anyone has any suggestions, or knows how to fix/cut air inflated lungs (for example if the trachea is closed before the opening of the thoracic cavity or so) I would be extremely grateful for the advise. The idea is to use these sections for confocal microscopy. Thank You All in advance. -- Alejandro Ortiz Stern M.D. Ph.D., Postdoctoral Fellow Institut National de la Sant? et de la Recherche M?dicale, E0344 Universit? de Nice-Sophia-Antipolis Institut de Pharmacologie Mol?culaire et Cellulaire 660 Route des Lucioles - 06560 Valbonne - FRANCE TEL: 33 (4) 93 95 77 80 - FAX: 33 (4) 93 95 77 08 From rjbuesa <@t> yahoo.com Tue Jun 6 10:41:56 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jun 6 10:42:05 2006 Subject: [Histonet] Yellow Walls in the Lab In-Reply-To: Message-ID: <20060606154156.44264.qmail@web61213.mail.yahoo.com> Wanda: Probably this is a combination between your lab environment and the type/quality of the paint used. Try first to find out the type of paint used and if it is of the alkyd type. White alkid wall paints become yellowish when there is not enough light and also in the presence of ammonia and chlorine. Try to find out the amount of light in your lab and note if yellowing is more acute in the less exposed to light areas. Sometimes ammonia is used as a "sectioning helper" of hard blocks and chlorine is sometimes used to clean/disinfect counter surfaces. A combination of all these factors can be the cause for your wall yellowing. Hope this will help you. Ren? J. Smith Wanda wrote: Good Morning Netters, As much as I hate to admit this...the walls in my lab where the tissue processors, embedding center, UPS for the embedding center, and microtomes are, they are turning yellow. It has been happening gradually, however now when the histotechs wipe the counters each day, the paper towel is yellow. It almost looks like nicotine tar, but they assure me they are not smoking in there in the mornings!!! My lab is 3 years old and they designed it with adequate ventilation to remove fumes, but I'm at a loss on this one. Please help!!!!! Wanda _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ring'em or ping'em. Make PC-to-phone calls as low as 1?/min with Yahoo! Messenger with Voice. From tp2 <@t> medicine.wisc.edu Tue Jun 6 10:43:24 2006 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Tue Jun 6 10:43:59 2006 Subject: [Histonet] TDP52, EZH2, Hepsin Message-ID: Hi Everybody, I was just wondering if anybody had any experience with staining FFPE Human prostate for TDP52, EZH2, or Hepsin. I've given EZH2 a few tries now and the only thing that ever stains cytoplasm of smooth muscle, not the nuclei of cancer cells. Anyway, any information would be greatly appreciated. Have a great week. Tom Pier From pmarcum <@t> vet.upenn.edu Tue Jun 6 11:19:34 2006 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Tue Jun 6 11:19:44 2006 Subject: [Histonet] Slide stainers Message-ID: <6.1.1.1.2.20060606115001.019c2ff8@mail.vet.upenn.edu> Good Afternoon to All, We are planning the Pennsylvania State Histology Meeting in October. We are attempting to put together an option for several of the companies to do a special stains explanation of benefits and features for each in PowerPoint. Hopefully to help those who are looking for a special stain instrument or just need more information to see the differences. This would help with which instrument does which stains or has specials we might not think of routinely. We have a list of the following companies Sakura, TBS, Leica, Thermo Shandon, Hacker, Ventana who currently have instruments. Did we miss anyone? If the companies wish to reply directly with comments or to commit to this format please contact either myself or Gloria Limetti ASAP so we can change or improve this idea. Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From gcallis <@t> montana.edu Tue Jun 6 11:23:26 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Jun 6 11:23:44 2006 Subject: [Histonet] Help with Lung architecture In-Reply-To: <44859D6D.7060109@ipmc.cnrs.fr> References: <44859D6D.7060109@ipmc.cnrs.fr> Message-ID: <6.0.0.22.1.20060606100824.01b3d1d8@gemini.msu.montana.edu> I presume you are working with mice? Since you say you have no idea about sucess with OCT or fixed lungs, you could try filling the lungs with OCT, then doing frozen sections and looking for the cells? The same for filling with formalin or paraformaldehyde? Howeer, with the latter fixation and wanting to do CLSM, you will have increased autofluorescence. With lavage, you are instilling fluid then pulling the fluid with cells back out - basically a rinsing technic that probably mechanically dislodges the cells (You did not say what cells you are looking for? alveolar macrophages??? ). We stain OCT filled lungs for many kinds of cells using immunofluorescence staining. You can try vibrating microtome sections on air inflated lungs, but these will be thicker. One lab that specializes in respiratory diseases and works with mice has great success with this, and uses the autofluorescence levels as a counterfluorescence for cells stained using an antibody conjugated to red fluorophore - they obtain wonderful z stacks with this technic on sections that vary from 50 um and up. They use a Leica vibrating microtome. The only way I have success with air filled lungs is snap freezing the lungs, then cryosectioning with Instrumedics Cryojane tape transfer method - you can do any kind of staining after this type of cryosectioning. The instrument is expensive but highly useful for problematic tissues. You can go to their website and look at the instrument. My experience with air inflated lungs and frozen sections using standard cryotomy is a total failure, the lungs crumble like sand and all morphology is lost. At 09:21 AM 6/6/2006, you wrote: >Hi, > >I am currently searching for a protocol to maintain Lung structure >intact while avoiding intra tracheal instillation of liquids since I am >very interested in cells that lie in the outer-most layers of the lung >(cells that would normally become detached upon lung lavage) Or if such >cells remain attached during paraformaldehyde instillation or OCT >instillation that would also be very valuable information (I have no idea). > >If anyone has any suggestions, or knows how to fix/cut air inflated >lungs (for example if the trachea is closed before the opening of the >thoracic cavity or so) I would be extremely grateful for the advise. > >The idea is to use these sections for confocal microscopy. > >Thank You All in advance. > >-- >Alejandro Ortiz Stern M.D. Ph.D., >Postdoctoral Fellow >Institut National de la Sant? et de la Recherche M?dicale, E0344 >Universit? de Nice-Sophia-Antipolis >Institut de Pharmacologie Mol?culaire et Cellulaire >660 Route des Lucioles - 06560 Valbonne - FRANCE >TEL: 33 (4) 93 95 77 80 - FAX: 33 (4) 93 95 77 08 > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From ACortez <@t> hei.org Tue Jun 6 11:33:27 2006 From: ACortez <@t> hei.org (Cortez, Ana) Date: Tue Jun 6 11:33:33 2006 Subject: [Histonet] Microtomr servicing and repair Message-ID: <87449E4A2B01DA47B29424CE5D6E0F8319167A@hi0sml1.hei.org> Hi everybody, I would like to know if there is another company for microtome's repair and service, besides McBain (in California). Any suggestion would be appreciated. Thank you all. Have a great day!! Ana Cortez House Ear Institute 2100 West Third Street, Los Angeles, CA 90057 Tel: (213) 483-4431 Fax:(213) 413-6739 acortez@hei.com From stern <@t> ipmc.cnrs.fr Tue Jun 6 11:44:22 2006 From: stern <@t> ipmc.cnrs.fr (Alejandro Ortiz Stern) Date: Tue Jun 6 11:39:38 2006 Subject: [Histonet] Help with Lung architecture In-Reply-To: <6.0.0.22.1.20060606100824.01b3d1d8@gemini.msu.montana.edu> References: <44859D6D.7060109@ipmc.cnrs.fr> <6.0.0.22.1.20060606100824.01b3d1d8@gemini.msu.montana.edu> Message-ID: <4485B0E6.1020009@ipmc.cnrs.fr> Yes, I forgot to mention that I will be working with mice. I will be looking for both alveolar macrophages and dendritic cells, but my main concern is that although it is not a lavage per se, the instillation procedure might dislodge the cells on the "way in". I will look into the vibrating microtome to see if such an apparatus exists here, since I am pretty sure they will not buy one only for this particular line of research. Thank you again for the help and the prompt response. Gayle Callis wrote: > I presume you are working with mice? Since you say you have no idea > about sucess with OCT or fixed lungs, you could try filling the lungs > with OCT, then doing frozen sections and looking for the cells? The > same for filling with formalin or paraformaldehyde? Howeer, with the > latter fixation and wanting to do CLSM, you will have increased > autofluorescence. > > With lavage, you are instilling fluid then pulling the fluid with > cells back out - basically a rinsing technic that probably > mechanically dislodges the cells (You did not say what cells you are > looking for? alveolar macrophages??? ). We stain OCT filled lungs > for many kinds of cells using immunofluorescence staining. > > You can try vibrating microtome sections on air inflated lungs, but > these will be thicker. One lab that specializes in respiratory > diseases and works with mice has great success with this, and uses the > autofluorescence levels as a counterfluorescence for cells stained > using an antibody conjugated to red fluorophore - they obtain > wonderful z stacks with this technic on sections that vary from 50 um > and up. They use a Leica vibrating microtome. > > The only way I have success with air filled lungs is snap freezing the > lungs, then cryosectioning with Instrumedics Cryojane tape transfer > method - you can do any kind of staining after this type of > cryosectioning. The instrument is expensive but highly useful for > problematic tissues. You can go to their website and look at the > instrument. > > My experience with air inflated lungs and frozen sections using > standard cryotomy is a total failure, the lungs crumble like sand and > all morphology is lost. > > > At 09:21 AM 6/6/2006, you wrote: >> Hi, >> >> I am currently searching for a protocol to maintain Lung structure >> intact while avoiding intra tracheal instillation of liquids since I am >> very interested in cells that lie in the outer-most layers of the lung >> (cells that would normally become detached upon lung lavage) Or if such >> cells remain attached during paraformaldehyde instillation or OCT >> instillation that would also be very valuable information (I have no >> idea). >> >> If anyone has any suggestions, or knows how to fix/cut air inflated >> lungs (for example if the trachea is closed before the opening of the >> thoracic cavity or so) I would be extremely grateful for the advise. >> >> The idea is to use these sections for confocal microscopy. >> >> Thank You All in advance. >> >> -- >> Alejandro Ortiz Stern M.D. Ph.D., >> Postdoctoral Fellow >> Institut National de la Sant? et de la Recherche M?dicale, E0344 >> Universit? de Nice-Sophia-Antipolis >> Institut de Pharmacologie Mol?culaire et Cellulaire >> 660 Route des Lucioles - 06560 Valbonne - FRANCE >> TEL: 33 (4) 93 95 77 80 - FAX: 33 (4) 93 95 77 08 >> >> >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 > 406 994-4303 (FAX) > > > > -- Alejandro Ortiz Stern M.D. Ph.D., Postdoctoral Fellow Institut National de la Sant? et de la Recherche M?dicale, E0344 Universit? de Nice-Sophia-Antipolis Institut de Pharmacologie Mol?culaire et Cellulaire 660 Route des Lucioles - 06560 Valbonne - FRANCE TEL: 33 (4) 93 95 77 80 - FAX: 33 (4) 93 95 77 08 From cormier <@t> MIT.EDU Tue Jun 6 12:37:44 2006 From: cormier <@t> MIT.EDU (Kathy Cormier) Date: Tue Jun 6 12:37:59 2006 Subject: [Histonet] trying to locate MSDS for AO polishing compound Message-ID: <001401c6898f$eb785ba0$92003712@mit.edu> Hi Netters, I have just spent a fruitless hour hunting the MSDS net for a list of the ingredients for American Optical "AOlite polishing compound" circa 1970 ish? Yes, I foolishly found a bottle in deep storage. Yes, probably it's just mineral oil and some kind of grit, but, I need to dispose of this properly. AO has been out of business for a bit, so no help there, google no help, siri msds no help, ilpi no help, etc. I have tried all the MSDS websites that I know of, and there are many, and no luck...Anyone out there have any more hints/help? Thanks! Kathy Div Comp Med DCM MIT From RCHIOVETTI <@t> aol.com Tue Jun 6 12:59:29 2006 From: RCHIOVETTI <@t> aol.com (RCHIOVETTI@aol.com) Date: Tue Jun 6 12:59:48 2006 Subject: [Histonet] trying to locate MSDS for AO polishing compound Message-ID: <3f8.353dd36.31b71c81@aol.com> In a message dated 6/6/2006 10:38:35 AM US Mountain Standard Time, cormier@MIT.EDU writes: > I have just spent a fruitless hour hunting the MSDS net for a list of the > ingredients for American Optical "AOlite polishing compound" circa 1970 ish? > Yes, I foolishly found a bottle in deep storage. Yes, probably it's just > mineral oil and some kind of grit, but, I need to dispose of this properly. AO has > been out of business for a bit, so no help there, google no help, siri msds > no help, ilpi no help, etc. I have tried all the MSDS websites that I know > of, and there are many, and no luck...Anyone out there have any more > hints/help? Thanks! > > Kathy > Div Comp Med > DCM MIT > Hi Kathy, It's a long, *long* shot, but you might try calling Leica. AO was absorbed by Leica several years ago, back in the days before MSDS sheets were such a big deal, so info may be limited or nonexistent. The number is 1-800-248-0123. Ask for Don Birgerson. I don't think I've been able to stump Don yet, after all these years! Cheers, Bob Robert (Bob) Chiovetti, Ph.D. Owner The Microscope Works Arizona's Microscopy Resource 132 North Elster Drive Tucson, AZ 85710-3212 USA Tel./Fax 520-546-4986 Manufacturers' Representative Member, Arizona Small Business Association - ASBA From gcallis <@t> montana.edu Tue Jun 6 13:50:23 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Jun 6 13:50:38 2006 Subject: [Histonet] trying to locate MSDS for AO polishing compound In-Reply-To: <001401c6898f$eb785ba0$92003712@mit.edu> References: <001401c6898f$eb785ba0$92003712@mit.edu> Message-ID: <6.0.0.22.1.20060606124549.01b1b578@gemini.msu.montana.edu> Is this for knife sharpening? Leica should help since they acquired AO, there are still some of these knife sharpening devices out there, and maybe still sold? Leica should know what is in that compound. I have CC this message to Don Birgerson at Lecia, he will probably be able to help you. At 11:37 AM 6/6/2006, you wrote: >Hi Netters, > >I have just spent a fruitless hour hunting the MSDS net for a list of the >ingredients for American Optical "AOlite polishing compound" circa 1970 >ish? Yes, I foolishly found a bottle in deep storage. Yes, probably it's >just mineral oil and some kind of grit, but, I need to dispose of this >properly. AO has been out of business for a bit, so no help there, google >no help, siri msds no help, ilpi no help, etc. I have tried all the MSDS >websites that I know of, and there are many, and no luck...Anyone out >there have any more hints/help? Thanks! Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From MElliott <@t> mrl.ubc.ca Tue Jun 6 14:22:36 2006 From: MElliott <@t> mrl.ubc.ca (Mark Elliott) Date: Tue Jun 6 14:23:34 2006 Subject: [Histonet] overnight HIER Message-ID: <4485738C020000D60001B845@mail.mrl.ubc.ca> Hi everyone. In a meeting this morning someone mentionned that they had been toold to do an overnight HIER treatment. They had never heard of this and wanted to know more. Is anyone doing this? Is it simply heating the slides at say 60C for 12-18 hours in a water bath or is there more to it-what buffer, what temp?? Any information would be greatly appreciated. Thanks Mark Elliott Vancouver BC From la.sebree <@t> hosp.wisc.edu Tue Jun 6 14:26:54 2006 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Tue Jun 6 14:27:00 2006 Subject: [Histonet] overnight HIER Message-ID: Mark, In one of the workshops I attended in Toronto ( I wish I could remember the name of the presenter), they mentioned doing this but not at 60 degrees C but a lower temp. like 37 or 45. Hopefully this person will see your inquiry and respond. I believe they did this for finicky antigens and tissues that were prone to come off the slides. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Elliott Sent: Tuesday, June 06, 2006 2:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] overnight HIER Hi everyone. In a meeting this morning someone mentionned that they had been toold to do an overnight HIER treatment. They had never heard of this and wanted to know more. Is anyone doing this? Is it simply heating the slides at say 60C for 12-18 hours in a water bath or is there more to it-what buffer, what temp?? Any information would be greatly appreciated. Thanks Mark Elliott Vancouver BC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tpmorken <@t> labvision.com Tue Jun 6 14:52:37 2006 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Tue Jun 6 14:52:45 2006 Subject: [Histonet] overnight HIER Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA20328D995@usca0082k08.labvision.apogent.com> Mark, Some people are using this method in order to preserve delicate tissue that is often damaged from high temperature AR. At lower temperatures delicate tissue seems to stay on the slide better. The proceedures I have seen consist of intervals of 16 - 20 hours at 70C or so. Here is a paper: Low-Temperature, Heat Mediated Antigen Retrieval (LTHMAR) on Archival Lymphoid Sections. Applied Immunohistochemistry and Molecular Morphology. 7(4):289, Decempber 1999 Tim Morken Lab Vision - Neomarkers www.labvision.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark Elliott Sent: Tuesday, June 06, 2006 12:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] overnight HIER Hi everyone. In a meeting this morning someone mentionned that they had been toold to do an overnight HIER treatment. They had never heard of this and wanted to know more. Is anyone doing this? Is it simply heating the slides at say 60C for 12-18 hours in a water bath or is there more to it-what buffer, what temp?? Any information would be greatly appreciated. Thanks Mark Elliott Vancouver BC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Tue Jun 6 15:24:49 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Jun 6 15:24:22 2006 Subject: [Histonet] Help with Lung architecture In-Reply-To: <44859D6D.7060109@ipmc.cnrs.fr> References: <44859D6D.7060109@ipmc.cnrs.fr> Message-ID: <4485E491.8030205@umdnj.edu> Perhaps you could use fumes from paraformaldehyde or acrolein to fix the cells in place. If you hook the animal up to a respirator and have some sort of resevoir with the fixing fumes in it (under a hood of course) you might be able to retain the cells you are interested in. Geoff Alejandro Ortiz Stern wrote: > Hi, > > I am currently searching for a protocol to maintain Lung structure > intact while avoiding intra tracheal instillation of liquids since I am > very interested in cells that lie in the outer-most layers of the lung > (cells that would normally become detached upon lung lavage) Or if such > cells remain attached during paraformaldehyde instillation or OCT > instillation that would also be very valuable information (I have no > idea). > > If anyone has any suggestions, or knows how to fix/cut air inflated > lungs (for example if the trachea is closed before the opening of the > thoracic cavity or so) I would be extremely grateful for the advise. > > The idea is to use these sections for confocal microscopy. > > Thank You All in advance. > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From JMacDonald <@t> mtsac.edu Tue Jun 6 16:00:10 2006 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Jun 6 16:00:37 2006 Subject: [Histonet] Microtomr servicing and repair In-Reply-To: <87449E4A2B01DA47B29424CE5D6E0F8319167A@hi0sml1.hei.org> Message-ID: Mikron Instruments does microtome service and repair. They also do processors and microscopes. Mikron Instruments (800) 377-5395 www.mikronet.com Jennifer "Cortez, Ana" Sent by: histonet-bounces@lists.utsouthwestern.edu 06/06/2006 09:33 AM To cc Subject [Histonet] Microtomr servicing and repair Hi everybody, I would like to know if there is another company for microtome's repair and service, besides McBain (in California). Any suggestion would be appreciated. Thank you all. Have a great day!! Ana Cortez House Ear Institute 2100 West Third Street, Los Angeles, CA 90057 Tel: (213) 483-4431 Fax:(213) 413-6739 acortez@hei.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Tue Jun 6 16:46:34 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Jun 6 16:46:45 2006 Subject: [Histonet] overnight HIER In-Reply-To: References: Message-ID: <6.0.0.22.1.20060606154352.01b435e8@gemini.msu.montana.edu> Mark, Linda is correct on this and Jules Elias has a publication on this in J of Histechnology, low heat antigen retrieval. Access the publication via the NSH office at histo@NSH.org. I do not recall the buffer nor antigens he was retrieving, and not sure of the year of publication. At 01:26 PM 6/6/2006, you wrote: >Mark, > >In one of the workshops I attended in Toronto ( I wish I could remember >the name of the presenter), they mentioned doing this but not at 60 >degrees C but a lower temp. like 37 or 45. Hopefully this person will >see your inquiry and respond. I believe they did this for finicky >antigens and tissues that were prone to come off the slides. > >Linda Sebree, HT(ASCP) >University of Wisconsin Hospital & Clinics >IHC/ISH Laboratory >A4/204-3224 >600 Highland Ave. >Madison, WI 53792 >(608)265-6596 >FAX: (608)262-7174 > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mark >Elliott >Sent: Tuesday, June 06, 2006 2:23 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] overnight HIER > > >Hi everyone. In a meeting this morning someone mentionned that they had >been toold to do an overnight HIER treatment. They had never heard of >this and wanted to know more. Is anyone doing this? Is it simply >heating the slides at say 60C for 12-18 hours in a water bath or is >there more to it-what buffer, what temp?? Any information would be >greatly appreciated. >Thanks >Mark Elliott >Vancouver BC > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From jnocito <@t> satx.rr.com Tue Jun 6 18:29:58 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Jun 6 18:30:11 2006 Subject: [Histonet] Automation Ventana vs. Dako References: Message-ID: <002c01c689c1$212ba640$0b69ce44@yourxhtr8hvc4p> flaming time. If the whole idea of a machine is to walk away, why would anyone apply the primary manually? That defeats the purpose of the machine. If I have to apply one reagent manually, might as well apply it all manually. As far as using the refillable dispensers, you can only do that a certain number of times before you; A-use a new dispenser or B- use the same dispenser, but replace the barcode label. Either way, you are adding an additional cost that isn't formulated into the equation when Ventana gives someone a cost/slide. I call that "creative accounting". No, I am not a fan of Ventana ( can you tell?), but I have several friends that work for Ventana. It's not the people, just the corporate mentality I can't digest. So you see, no matter how you slice it, Ventana's machines do not have the "ability" to even be a partially open system. I know I'll be hearing about this again. So, as not to be a coward, here's my signature block. Hell, I'll even add the telephone number so y'all won't have to look it up. Those of you know where I work, I'll be expecting a phone call either to me or my CEO. You know who you are. I've been told by my CEO before to back off. It hasn't worked before, it won't happen this time either. One other thing. As always, my opinions do not reflect the opinions of my employers and even some of my co-workers, lawyers, housekeeping, used car salepeople, etc. Commence the flaming. (Is it Friday yet?) Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX 210-892-3747 (that's my direct line) ----- Original Message ----- From: "Sebree Linda A." To: "Carla Frenchko" ; "Heather Renko" ; Sent: Tuesday, June 06, 2006 7:52 AM Subject: RE: [Histonet] Automation Ventana vs. Dako Misconception here: you can run antibodies from any company on Ventana instruments either by putting them in a user-fillable Ventana dispenser or by manually applying the antibody in a titration mode run. We do both of these as well as use some of Ventana's antibodies. The only thing you are "married to" is their detection chemistries, biotin block (if using) and amplification (if using). You can employ your own enzyme digestion reagent and counterstain if desired. If performing antigen retrieval on line, you also are wed to Ventana's Cell Conditioning solutions (HIER buffers)....although nothing prevents you from using your own HIER methods and reagents off-line which is what we do when using our NexES instrument. Just trying to clear a few things up. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carla Frenchko Sent: Tuesday, June 06, 2006 6:53 AM To: Heather Renko; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Automation Ventana vs. Dako With all due respect to Heather; but the reason the Ventana is not really an "open" system is that you are "married" to a particular vendor's antibodies and detection. In a true "open" system most any company's antibodies will run with the instrument's detection (specifically speaking of both the Dako and Biogenex instruments having run both). Sincerely, Carla -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heather Renko Sent: Monday, June 05, 2006 10:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automation Ventana vs. Dako As an ex Ventana Tech Rep (not sales) I can say that you really have to judge what instrument is best suited for your volume and your staffing. Ventana is a great company with a great product but, very expensive. Can you rely on it-yes, Can any tech run it with minimal training-yes. Have they had lot issues with detection, sure but name a company that hasn't run into that at some point. Dako on the other hand is very reliable and has the market on good antibodies. Can anyone run it-not without a bit of hands training. It seems to be more affordable than Ventana but, you have to have a good staff that can consistently reproduce steps and make up dilutions. With Ventana you can cut, dry, put it on the instrument and then walk away-reproducible and consistent pretty much every time. With Ventana you can also create an open system and run some great Dako antibodies or any other vendors products for that fact. Your only marrried to their detection. Both companies seem to have good tech support and Dako has allot of really sharp publications to get you off and running. Unfortunately in today's histology labs we are seeing such an increase in staffing changes that it is sometimes better to pay the extra money and let the Ventana Benchmark do the work and not worry about it. On the other hand if you have a good tech staff that can be "cooks in the kitchen" than Dako seems to be a pretty good instrument too. I welcome you to listen to your peers-its just simply finding out which company will suit your needs and what your budget will endure. Just my un-bias two cents. I wish you all the best in your decison making process. Heather __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.8.2/357 - Release Date: 6/6/2006 From jnocito <@t> satx.rr.com Tue Jun 6 18:32:54 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Jun 6 18:33:02 2006 Subject: [Histonet] Yellow Walls in the Lab References: <20060606154156.44264.qmail@web61213.mail.yahoo.com> Message-ID: <004201c689c1$89414f50$0b69ce44@yourxhtr8hvc4p> gee, we have purple walls ( the interior designer calls it eggplant). I'll let y'all know what color we get in a couple of years, maybe sooner. Remember Bent plumbing? That's what I'm talking about. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Rene J Buesa" To: "Smith Wanda" ; Sent: Tuesday, June 06, 2006 10:41 AM Subject: Re: [Histonet] Yellow Walls in the Lab > Wanda: > Probably this is a combination between your lab environment and the > type/quality of the paint used. > Try first to find out the type of paint used and if it is of the alkyd > type. White alkid wall paints become yellowish when there is not enough > light and also in the presence of ammonia and chlorine. > Try to find out the amount of light in your lab and note if yellowing is > more acute in the less exposed to light areas. Sometimes ammonia is used > as a "sectioning helper" of hard blocks and chlorine is sometimes used to > clean/disinfect counter surfaces. > A combination of all these factors can be the cause for your wall > yellowing. > Hope this will help you. > Ren? J. > > Smith Wanda wrote: > Good Morning Netters, > As much as I hate to admit this...the walls in my lab where the tissue > processors, embedding center, UPS for the embedding center, and microtomes > are, they are turning yellow. It has been happening gradually, however now > when the histotechs wipe the counters each day, the paper towel is yellow. > It almost looks like nicotine tar, but they assure me they are not smoking > in there in the mornings!!! > My lab is 3 years old and they designed it with adequate ventilation to > remove fumes, but I'm at a loss on this one. Please help!!!!! > Wanda > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Ring'em or ping'em. Make PC-to-phone calls as low as 1?/min with Yahoo! > Messenger with Voice. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.394 / Virus Database: 268.8.2/357 - Release Date: 6/6/2006 > > From LuckG <@t> empirehealth.org Tue Jun 6 18:57:14 2006 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Tue Jun 6 18:57:21 2006 Subject: [Histonet] Job Opening Message-ID: <6BB8BC4519AAB844B174FC739A679BBC1C304F@IRMEXCH01.irm.inhs.org> Hello all, We have a full time, days, M-F histotech position open here at Deaconess Medical Center in Spokane, Washington. If you'd like more specifics about the job duties and the lab environment in general please contact me personally. I have included 3 web links below you can just click on to go to the websites where you can learn more about our Empire Health Services, Deaconess and Spokane and the surrounding area. We would like to fill this position by August 1st. Thanks!! www.visitspokane.c om www.deaconessmc.org www.empirehealth.org Greg Luck Anatomic Path Spvr Deaconess Med Cntr 800 W. 5th Ave Spokane, WA 99204 Offc 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmc.org From cbass <@t> bidmc.harvard.edu Wed Jun 7 00:13:01 2006 From: cbass <@t> bidmc.harvard.edu (Caroline Bass) Date: Wed Jun 7 00:13:18 2006 Subject: [Histonet] dapi and GFP problem problem Message-ID: <03BAA0B6-AA0B-4CAD-9A99-99CEF1F0A916@bidmc.harvard.edu> Hey, I have a GFP labeled g-protein coupled receptor that I am expressing in HEK 293 cells. I cultured the cells on collagen coated glass chamber slides, fixed the cells with 4% PFA, and then coverslipped with Prolong Gold antifade containing dapi. Before I coverslipped I checked the slide on a standard fluorescent microscope to make sure everything was expressing correctly. My transduced cells had a nice signal and my control was black. The day after coverslipping my control cells showed a noticeable green signal, not quite a bright as the GFP cells, but it is apparent. I took confocal pics and there was clearly some green in my control cells. Does anyone have a suggestions? I figure one of two things happened. Either some cells slid around when I pressed down on the coverslip or maybe I am getting some sort of bleedthrough. I am not very good with the confocal yet, so I don't know if I did something wrong here. Does anyone have a good protocol for growing 293 cells on glass slides? I have heard there of some super adherent clones of 293 that I would like to try, although I haven't found a source for them. Any and all suggestions would be appreciated. Thanks, Caroline From elke.genersch <@t> rz.hu-berlin.de Wed Jun 7 04:01:09 2006 From: elke.genersch <@t> rz.hu-berlin.de (Dr. Elke Genersch) Date: Wed Jun 7 04:01:27 2006 Subject: [Histonet] sectioning bee's heads and mites Message-ID: <5.1.0.14.1.20060607105605.02a312a8@popserv.rz.hu-berlin.de> For in situ hybridization we need to obtain sections from the heads of bees (not just brain!) and from whole mites. Has anyone experience with fixation, embedding and sectioning of such material ? Elke Genersch, PhD Elke Genersch, Ph.D. Vice Director Institute for Bee Research Friedrich-Engels-Stra?e 32 D - 16540 Hohen Neuendorf Tel.: +49 - (0)3303 - 293833 Fax: +49 - (0)3303 - 293840 e-mail: elke.genersch@rz.hu-berlin.de homepage: www.honigbiene.de Associated Member of the Zentrum f?r Infektionsbiologie und Immunit?t = ZIBI www.biologie.hu-berlin.de/~ZIBI/ From mike.kirby <@t> nhls.ac.za Wed Jun 7 04:57:22 2006 From: mike.kirby <@t> nhls.ac.za (Mike Kirby) Date: Wed Jun 7 04:49:30 2006 Subject: [Histonet] Neutralisation of 10% formalin using ammonia Message-ID: <452D0F6B16AA6E4092F6D3E9A9D97A6224EFD8@nhlsgpm002.NHLS.AC.ZA> Dear Histonetters. The method of neutralising 10% formalin using concentrated ammonia solution has been well documented, and no doubt used by many Labs to dispose of their formalin waste. My first question is, how does one verify that all the formalin has been neutralised? One reference states that when the pH changes from 6 to 8, then sufficient ammonia has been added, and by implication, all the formalin should have been neutralised. My second question is, would Schiffs Reagent be of any use as a double check in this methodology, because as we all know, it turns pink, even at very low concentrations of formalin, or would the final product, Hexamethylenetetramine, also react with Schiffs? Has anyone pursued this avenue? Mr.M.Kirby Johannesburg South Africa. ********************************************************************************** The views expressed in this email are, unless otherwise stated, those of the author and not those of the National Health Laboratory Services or its management. The information in this e-mail is confidential and is intended solely for the addressee. Access to this e-mail by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken or omitted in reliance on this, is prohibited and may be unlawful. Whilst all reasonable steps are taken to ensure the accuracy and integrity of information and data transmitted electronically and to preserve the confidentiality thereof, no liability or responsibility whatsoever is accepted if information or data is, for whatever reason, corrupted or does not reach its intended destination. *********************************************************************************** From Don.Birgerson <@t> leica-microsystems.com Wed Jun 7 07:40:30 2006 From: Don.Birgerson <@t> leica-microsystems.com (Don.Birgerson@leica-microsystems.com) Date: Wed Jun 7 07:40:42 2006 Subject: [Histonet] trying to locate MSDS for AO polishing compound In-Reply-To: <001401c6898f$eb785ba0$92003712@mit.edu> Message-ID: Hi Kathy, I have been following this question and thought I would voice my thoughts. This "AOlite" polishing compound did not come from our microtome knife sharpeners. I believe it was a product name of material to cut and polish prescription eyeglasses. Being from the '70s, there won't be a MSDS for this material. I am trying to confirm my guess by trying to contact any of AO's older opthalmic people. If you have a "eyeglass or Opthalmic dept" , you might see if they remember. Best regards, Don Don Birgerson Leica Microsystems Technical Assistance Center Don.Birgerson@Leica-Microsystems.Com 1-800-248-0123 ext 5918 "Kathy Cormier" Sent by: To histonet-bounces@ lists.utsouthwest cc ern.edu Subject [Histonet] trying to locate MSDS 06/06/2006 12:37 for AO polishing compound PM Hi Netters, I have just spent a fruitless hour hunting the MSDS net for a list of the ingredients for American Optical "AOlite polishing compound" circa 1970 ish? Yes, I foolishly found a bottle in deep storage. Yes, probably it's just mineral oil and some kind of grit, but, I need to dispose of this properly. AO has been out of business for a bit, so no help there, google no help, siri msds no help, ilpi no help, etc. I have tried all the MSDS websites that I know of, and there are many, and no luck...Anyone out there have any more hints/help? Thanks! Kathy Div Comp Med DCM MIT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From la.sebree <@t> hosp.wisc.edu Wed Jun 7 08:06:07 2006 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Wed Jun 7 08:06:13 2006 Subject: [Histonet] Automation Ventana vs. Dako Message-ID: Joe, We do manual applications of the antibody when it needs to be made up fresh or the frequency of that particular antibody is so low as to make it not economical to purchase a user-fillable dispenser. Our threshold of whether we automate an antibody is if we are getting enough requests so that we would use up the dispenses in a dispenser (100 or 250, depending on which you purchase) by the time the dispenser expires (I think its ~ 18 months). Having the option to automate an antibody or not is a nice flexibility feature although I know some/many? labs just automate every antibody they run; we choose not to. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Tuesday, June 06, 2006 6:30 PM To: Sebree Linda A.; Carla Frenchko; Heather Renko; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Automation Ventana vs. Dako flaming time. If the whole idea of a machine is to walk away, why would anyone apply the primary manually? That defeats the purpose of the machine. If I have to apply one reagent manually, might as well apply it all manually. As far as using the refillable dispensers, you can only do that a certain number of times before you; A-use a new dispenser or B- use the same dispenser, but replace the barcode label. Either way, you are adding an additional cost that isn't formulated into the equation when Ventana gives someone a cost/slide. I call that "creative accounting". No, I am not a fan of Ventana ( can you tell?), but I have several friends that work for Ventana. It's not the people, just the corporate mentality I can't digest. So you see, no matter how you slice it, Ventana's machines do not have the "ability" to even be a partially open system. I know I'll be hearing about this again. So, as not to be a coward, here's my signature block. Hell, I'll even add the telephone number so y'all won't have to look it up. Those of you know where I work, I'll be expecting a phone call either to me or my CEO. You know who you are. I've been told by my CEO before to back off. It hasn't worked before, it won't happen this time either. One other thing. As always, my opinions do not reflect the opinions of my employers and even some of my co-workers, lawyers, housekeeping, used car salepeople, etc. Commence the flaming. (Is it Friday yet?) Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX 210-892-3747 (that's my direct line) ----- Original Message ----- From: "Sebree Linda A." To: "Carla Frenchko" ; "Heather Renko" ; Sent: Tuesday, June 06, 2006 7:52 AM Subject: RE: [Histonet] Automation Ventana vs. Dako Misconception here: you can run antibodies from any company on Ventana instruments either by putting them in a user-fillable Ventana dispenser or by manually applying the antibody in a titration mode run. We do both of these as well as use some of Ventana's antibodies. The only thing you are "married to" is their detection chemistries, biotin block (if using) and amplification (if using). You can employ your own enzyme digestion reagent and counterstain if desired. If performing antigen retrieval on line, you also are wed to Ventana's Cell Conditioning solutions (HIER buffers)....although nothing prevents you from using your own HIER methods and reagents off-line which is what we do when using our NexES instrument. Just trying to clear a few things up. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carla Frenchko Sent: Tuesday, June 06, 2006 6:53 AM To: Heather Renko; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Automation Ventana vs. Dako With all due respect to Heather; but the reason the Ventana is not really an "open" system is that you are "married" to a particular vendor's antibodies and detection. In a true "open" system most any company's antibodies will run with the instrument's detection (specifically speaking of both the Dako and Biogenex instruments having run both). Sincerely, Carla -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heather Renko Sent: Monday, June 05, 2006 10:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automation Ventana vs. Dako As an ex Ventana Tech Rep (not sales) I can say that you really have to judge what instrument is best suited for your volume and your staffing. Ventana is a great company with a great product but, very expensive. Can you rely on it-yes, Can any tech run it with minimal training-yes. Have they had lot issues with detection, sure but name a company that hasn't run into that at some point. Dako on the other hand is very reliable and has the market on good antibodies. Can anyone run it-not without a bit of hands training. It seems to be more affordable than Ventana but, you have to have a good staff that can consistently reproduce steps and make up dilutions. With Ventana you can cut, dry, put it on the instrument and then walk away-reproducible and consistent pretty much every time. With Ventana you can also create an open system and run some great Dako antibodies or any other vendors products for that fact. Your only marrried to their detection. Both companies seem to have good tech support and Dako has allot of really sharp publications to get you off and running. Unfortunately in today's histology labs we are seeing such an increase in staffing changes that it is sometimes better to pay the extra money and let the Ventana Benchmark do the work and not worry about it. On the other hand if you have a good tech staff that can be "cooks in the kitchen" than Dako seems to be a pretty good instrument too. I welcome you to listen to your peers-its just simply finding out which company will suit your needs and what your budget will endure. Just my un-bias two cents. I wish you all the best in your decison making process. Heather __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.8.2/357 - Release Date: 6/6/2006 From Dorothy.L.Webb <@t> HealthPartners.Com Wed Jun 7 09:21:10 2006 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Wed Jun 7 09:21:20 2006 Subject: [Histonet] Tape coverslipping Message-ID: <0E394B648E5284478A6CCB78E5AFDA270171FE04@hpes1.HealthPartners.int> I have only used the tape method for automated coverslippers, but, have not seen any problems with the tape coming off, etc. even after MANY years!! I think what everyone has to make certain is that the pressure is adjusted high enough on the "bar" that pushes the tape onto the slide and make certain enough xylene is flowing, more than just a few drops. We had our biomed department adjust the tension on the spring so the bar produced more pressure when it went across the tape!! Having never used the glass coverslippers, but hearing enough concerns, I am not goin there until my tape coverslipper "kicks the bucket" !! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From rjbuesa <@t> yahoo.com Wed Jun 7 09:39:56 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 7 09:40:00 2006 Subject: [Histonet] Neutralisation of 10% formalin using ammonia In-Reply-To: <452D0F6B16AA6E4092F6D3E9A9D97A6224EFD8@nhlsgpm002.NHLS.AC.ZA> Message-ID: <20060607143956.14374.qmail@web61224.mail.yahoo.com> Mr. M: Schiff's reagent is perhaps the best, most sensitive and available reagent in any lab to test for the presence of aldehydes. If you "neutralized" formalin turns purplish with the addition of Schiff's reagent, it is not totally neutralized. That was my reagent of choice when I tested different formalin neutralizing products. Sakura provides a kit containing Schiff's reagent to test their neutralizing product. Ren? J. Mike Kirby wrote: Dear Histonetters. The method of neutralising 10% formalin using concentrated ammonia solution has been well documented, and no doubt used by many Labs to dispose of their formalin waste. My first question is, how does one verify that all the formalin has been neutralised? One reference states that when the pH changes from 6 to 8, then sufficient ammonia has been added, and by implication, all the formalin should have been neutralised. My second question is, would Schiffs Reagent be of any use as a double check in this methodology, because as we all know, it turns pink, even at very low concentrations of formalin, or would the final product, Hexamethylenetetramine, also react with Schiffs? Has anyone pursued this avenue? Mr.M.Kirby Johannesburg South Africa. ********************************************************************************** The views expressed in this email are, unless otherwise stated, those of the author and not those of the National Health Laboratory Services or its management. The information in this e-mail is confidential and is intended solely for the addressee. Access to this e-mail by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken or omitted in reliance on this, is prohibited and may be unlawful. Whilst all reasonable steps are taken to ensure the accuracy and integrity of information and data transmitted electronically and to preserve the confidentiality thereof, no liability or responsibility whatsoever is accepted if information or data is, for whatever reason, corrupted or does not reach its intended destination. *********************************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From LGaliotto <@t> nch.org Wed Jun 7 09:47:53 2006 From: LGaliotto <@t> nch.org (Galiotto, Laura) Date: Wed Jun 7 09:47:49 2006 Subject: [Histonet] Rapid processing fro breast core biopsies Message-ID: <270614B321ACB44D8C1D91F4F921FDC3036CBDA5@NCH01EX02.nch.org> Good morning fellow histotechs. Is there anyone that is currently processing breast core biopsies size 2.5-3 cm by .5 cm using any of the microwaves or the Sakura Express? If so please share your process including the TAT. Laura Galiotto, HT (ASCP) Histology Facilitator Northwest Community Hospital 800 West Central Road Arlington Heights, Il 60005 847-618-6190 ******************** PLEASE NOTE ******************** This E-mail/telefax message and any documents accompanying this transmission may contain information that is privileged, confidential, and/or exempt from disclosure under applicable law and is intended for the addressee(s) named above. If you are not the intended addressee/recipient, you are hereby notified that any use of, disclosure, copying, distribution, or reliance on the contents of this E-Mail/telefax information is strictly prohibited and may result in legal action against you. Please reply to the sender advising of the error in transmission and immediately delete/destroy the message and any accompanying documents. Thank you. From mauger <@t> email.chop.edu Wed Jun 7 09:53:31 2006 From: mauger <@t> email.chop.edu (Joanne Mauger) Date: Wed Jun 7 09:54:09 2006 Subject: [Histonet] lysis of RBC in FFPE Message-ID: Hi, Hope someone can help. We need a method for lysing RBCs in FFPE human tissue for special stains, not IHC. It is extremely bloody tissue. Thanks, Jo >>> "Joe Nocito" 06/06/06 7:29 PM >>> flaming time. If the whole idea of a machine is to walk away, why would anyone apply the primary manually? That defeats the purpose of the machine. If I have to apply one reagent manually, might as well apply it all manually. As far as using the refillable dispensers, you can only do that a certain number of times before you; A-use a new dispenser or B- use the same dispenser, but replace the barcode label. Either way, you are adding an additional cost that isn't formulated into the equation when Ventana gives someone a cost/slide. I call that "creative accounting". No, I am not a fan of Ventana ( can you tell?), but I have several friends that work for Ventana. It's not the people, just the corporate mentality I can't digest. So you see, no matter how you slice it, Ventana's machines do not have the "ability" to even be a partially open system. I know I'll be hearing about this again. So, as not to be a coward, here's my signature block. Hell, I'll even add the telephone number so y'all won't have to look it up. Those of you know where I work, I'll be expecting a phone call either to me or my CEO. You know who you are. I've been told by my CEO before to back off. It hasn't worked before, it won't happen this time either. One other thing. As always, my opinions do not reflect the opinions of my employers and even some of my co-workers, lawyers, housekeeping, used car salepeople, etc. Commence the flaming. (Is it Friday yet?) Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX 210-892-3747 (that's my direct line) ----- Original Message ----- From: "Sebree Linda A." To: "Carla Frenchko" ; "Heather Renko" ; Sent: Tuesday, June 06, 2006 7:52 AM Subject: RE: [Histonet] Automation Ventana vs. Dako Misconception here: you can run antibodies from any company on Ventana instruments either by putting them in a user-fillable Ventana dispenser or by manually applying the antibody in a titration mode run. We do both of these as well as use some of Ventana's antibodies. The only thing you are "married to" is their detection chemistries, biotin block (if using) and amplification (if using). You can employ your own enzyme digestion reagent and counterstain if desired. If performing antigen retrieval on line, you also are wed to Ventana's Cell Conditioning solutions (HIER buffers)....although nothing prevents you from using your own HIER methods and reagents off-line which is what we do when using our NexES instrument. Just trying to clear a few things up. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carla Frenchko Sent: Tuesday, June 06, 2006 6:53 AM To: Heather Renko; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Automation Ventana vs. Dako With all due respect to Heather; but the reason the Ventana is not really an "open" system is that you are "married" to a particular vendor's antibodies and detection. In a true "open" system most any company's antibodies will run with the instrument's detection (specifically speaking of both the Dako and Biogenex instruments having run both). Sincerely, Carla -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heather Renko Sent: Monday, June 05, 2006 10:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automation Ventana vs. Dako As an ex Ventana Tech Rep (not sales) I can say that you really have to judge what instrument is best suited for your volume and your staffing. Ventana is a great company with a great product but, very expensive. Can you rely on it-yes, Can any tech run it with minimal training-yes. Have they had lot issues with detection, sure but name a company that hasn't run into that at some point. Dako on the other hand is very reliable and has the market on good antibodies. Can anyone run it-not without a bit of hands training. It seems to be more affordable than Ventana but, you have to have a good staff that can consistently reproduce steps and make up dilutions. With Ventana you can cut, dry, put it on the instrument and then walk away-reproducible and consistent pretty much every time. With Ventana you can also create an open system and run some great Dako antibodies or any other vendors products for that fact. Your only marrried to their detection. Both companies seem to have good tech support and Dako has allot of really sharp publications to get you off and running. Unfortunately in today's histology labs we are seeing such an increase in staffing changes that it is sometimes better to pay the extra money and let the Ventana Benchmark do the work and not worry about it. On the other hand if you have a good tech staff that can be "cooks in the kitchen" than Dako seems to be a pretty good instrument too. I welcome you to listen to your peers-its just simply finding out which company will suit your needs and what your budget will endure. Just my un-bias two cents. I wish you all the best in your decison making process. Heather __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.8.2/357 - Release Date: 6/6/2006 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJasper <@t> smdc.org Wed Jun 7 10:10:49 2006 From: TJasper <@t> smdc.org (Jasper, Thomas G.) Date: Wed Jun 7 10:11:06 2006 Subject: [Histonet] Automation Ventana vs. Dako Message-ID: <1A9F2A6C5762524799A816F1F09744CF1E0AC5@SCREECH.ntcampus.smdc.org> Hey Joe, No flaming, what you're saying has validity. In thinking all this over it's never one size fits all (see my last post). I agree the pricing is high, but for us (and others probably) the "true cost" works out all right (for now anyway). Please don't get me wrong I think Dako is good stuff and other folks are solidly behind different automated systems. I'd like to believe these systems have their good points as well. And you know competition is good for everyone, so there you have it. Keep at it Joe, guys like you keep things honest and healthy. Tom J. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Joe Nocito Sent: Tuesday, June 06, 2006 6:30 PM To: Sebree Linda A.; Carla Frenchko; Heather Renko; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Automation Ventana vs. Dako flaming time. If the whole idea of a machine is to walk away, why would anyone apply the primary manually? That defeats the purpose of the machine. If I have to apply one reagent manually, might as well apply it all manually. As far as using the refillable dispensers, you can only do that a certain number of times before you; A-use a new dispenser or B- use the same dispenser, but replace the barcode label. Either way, you are adding an additional cost that isn't formulated into the equation when Ventana gives someone a cost/slide. I call that "creative accounting". No, I am not a fan of Ventana ( can you tell?), but I have several friends that work for Ventana. It's not the people, just the corporate mentality I can't digest. So you see, no matter how you slice it, Ventana's machines do not have the "ability" to even be a partially open system. I know I'll be hearing about this again. So, as not to be a coward, here's my signature block. Hell, I'll even add the telephone number so y'all won't have to look it up. Those of you know where I work, I'll be expecting a phone call either to me or my CEO. You know who you are. I've been told by my CEO before to back off. It hasn't worked before, it won't happen this time either. One other thing. As always, my opinions do not reflect the opinions of my employers and even some of my co-workers, lawyers, housekeeping, used car salepeople, etc. Commence the flaming. (Is it Friday yet?) Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX 210-892-3747 (that's my direct line) ----- Original Message ----- From: "Sebree Linda A." To: "Carla Frenchko" ; "Heather Renko" ; Sent: Tuesday, June 06, 2006 7:52 AM Subject: RE: [Histonet] Automation Ventana vs. Dako Misconception here: you can run antibodies from any company on Ventana instruments either by putting them in a user-fillable Ventana dispenser or by manually applying the antibody in a titration mode run. We do both of these as well as use some of Ventana's antibodies. The only thing you are "married to" is their detection chemistries, biotin block (if using) and amplification (if using). You can employ your own enzyme digestion reagent and counterstain if desired. If performing antigen retrieval on line, you also are wed to Ventana's Cell Conditioning solutions (HIER buffers)....although nothing prevents you from using your own HIER methods and reagents off-line which is what we do when using our NexES instrument. Just trying to clear a few things up. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carla Frenchko Sent: Tuesday, June 06, 2006 6:53 AM To: Heather Renko; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Automation Ventana vs. Dako With all due respect to Heather; but the reason the Ventana is not really an "open" system is that you are "married" to a particular vendor's antibodies and detection. In a true "open" system most any company's antibodies will run with the instrument's detection (specifically speaking of both the Dako and Biogenex instruments having run both). Sincerely, Carla -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heather Renko Sent: Monday, June 05, 2006 10:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automation Ventana vs. Dako As an ex Ventana Tech Rep (not sales) I can say that you really have to judge what instrument is best suited for your volume and your staffing. Ventana is a great company with a great product but, very expensive. Can you rely on it-yes, Can any tech run it with minimal training-yes. Have they had lot issues with detection, sure but name a company that hasn't run into that at some point. Dako on the other hand is very reliable and has the market on good antibodies. Can anyone run it-not without a bit of hands training. It seems to be more affordable than Ventana but, you have to have a good staff that can consistently reproduce steps and make up dilutions. With Ventana you can cut, dry, put it on the instrument and then walk away-reproducible and consistent pretty much every time. With Ventana you can also create an open system and run some great Dako antibodies or any other vendors products for that fact. Your only marrried to their detection. Both companies seem to have good tech support and Dako has allot of really sharp publications to get you off and running. Unfortunately in today's histology labs we are seeing such an increase in staffing changes that it is sometimes better to pay the extra money and let the Ventana Benchmark do the work and not worry about it. On the other hand if you have a good tech staff that can be "cooks in the kitchen" than Dako seems to be a pretty good instrument too. I welcome you to listen to your peers-its just simply finding out which company will suit your needs and what your budget will endure. Just my un-bias two cents. I wish you all the best in your decison making process. Heather __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.8.2/357 - Release Date: 6/6/2006 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From vanann702 <@t> skmc.gov.ae Wed Jun 7 10:37:23 2006 From: vanann702 <@t> skmc.gov.ae (Anne Van Binsbergen) Date: Wed Jun 7 10:36:58 2006 Subject: [Histonet] Neutralisation of 10% formalin using ammonia References: <20060607143956.14374.qmail@web61224.mail.yahoo.com> Message-ID: hey Mike and Rene! 'camel queens' use Neutralex by Sakura - its the best!!! hope you are both well - in your respective corners of the Histonet!! ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Wed 2006/06/07 06:39 PM To: Mike Kirby; Histonet (E-mail) Subject: Re: [Histonet] Neutralisation of 10% formalin using ammonia Mr. M: Schiff's reagent is perhaps the best, most sensitive and available reagent in any lab to test for the presence of aldehydes. If you "neutralized" formalin turns purplish with the addition of Schiff's reagent, it is not totally neutralized. That was my reagent of choice when I tested different formalin neutralizing products. Sakura provides a kit containing Schiff's reagent to test their neutralizing product. Ren? J. Mike Kirby wrote: Dear Histonetters. The method of neutralising 10% formalin using concentrated ammonia solution has been well documented, and no doubt used by many Labs to dispose of their formalin waste. My first question is, how does one verify that all the formalin has been neutralised? One reference states that when the pH changes from 6 to 8, then sufficient ammonia has been added, and by implication, all the formalin should have been neutralised. My second question is, would Schiffs Reagent be of any use as a double check in this methodology, because as we all know, it turns pink, even at very low concentrations of formalin, or would the final product, Hexamethylenetetramine, also react with Schiffs? Has anyone pursued this avenue? Mr.M.Kirby Johannesburg South Africa. ********************************************************************************** The views expressed in this email are, unless otherwise stated, those of the author and not those of the National Health Laboratory Services or its management. The information in this e-mail is confidential and is intended solely for the addressee. Access to this e-mail by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken or omitted in reliance on this, is prohibited and may be unlawful. Whilst all reasonable steps are taken to ensure the accuracy and integrity of information and data transmitted electronically and to preserve the confidentiality thereof, no liability or responsibility whatsoever is accepted if information or data is, for whatever reason, corrupted or does not reach its intended destination. *********************************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Wed Jun 7 10:53:05 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Jun 7 10:53:23 2006 Subject: [Histonet] dapi and GFP problem problem In-Reply-To: <03BAA0B6-AA0B-4CAD-9A99-99CEF1F0A916@bidmc.harvard.edu> References: <03BAA0B6-AA0B-4CAD-9A99-99CEF1F0A916@bidmc.harvard.edu> Message-ID: <6.0.0.22.1.20060607093827.01b499f8@gemini.msu.montana.edu> You are probably experiencing autofluorescence induced the aldehyde fixation. You can try Molecular Probes Image - IT image enhancer that is supposed to get rid of the autofluorescence. It is important to note that Prolong Gold antifade is supposed to sit overnight and not sealed with fingernail polish, by curing overnight, this mounting media, the refractive index gradually increses as it cures. This may be why the control appeared black just after you mounted the coverslip. You can tell the CLSM to get rid of the autofluorescence too, some might consider this cheating, but it can be done. What may be better is to NOT fix the cells, and view them with CLSM - GFP is designed for use with living cells and you may find the GFP is even brighter. The Clontech manual called Living Colours tells you a great deal about handling cells, etc with GFP. That manual can be accessed online in pdf form. Unfixed cells can also be mounted with Prolong Gold antifade w/ DAPI - we do this with unfixed frozen sections to avoid all fixation or you can add a DAPI solution to the unfixed cells, and view them without coverslipping or coverslipping with PBS. At 11:13 PM 6/6/2006, you wrote: >Hey, > >I have a GFP labeled g-protein coupled receptor that I am expressing >in HEK 293 cells. I cultured the cells on collagen coated glass >chamber slides, fixed the cells with 4% PFA, and then coverslipped >with Prolong Gold antifade containing dapi. Before I coverslipped I >checked the slide on a standard fluorescent microscope to make sure >everything was expressing correctly. My transduced cells had a nice >signal and my control was black. The day after coverslipping my >control cells showed a noticeable green signal, not quite a bright as >the GFP cells, but it is apparent. I took confocal pics and there >was clearly some green in my control cells. > >Does anyone have a suggestions? I figure one of two things >happened. Either some cells slid around when I pressed down on the >coverslip or maybe I am getting some sort of bleedthrough. I am not >very good with the confocal yet, so I don't know if I did something >wrong here. Does anyone have a good protocol for growing 293 cells >on glass slides? I have heard there of some super adherent clones of >293 that I would like to try, although I haven't found a source for >them. > >Any and all suggestions would be appreciated. > >Thanks, > >Caroline > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From algranth <@t> u.arizona.edu Wed Jun 7 10:53:56 2006 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Wed Jun 7 10:54:10 2006 Subject: [Histonet] sectioning bee's heads and mites In-Reply-To: <5.1.0.14.1.20060607105605.02a312a8@popserv.rz.hu-berlin.de > Message-ID: <4.3.2.7.2.20060607081912.025b8a90@algranth.inbox.email.arizona.edu> Dr. Genersch, From time to time I have to section insects and it is difficult since their little bodies are hard on the outside and processing makes them harder. I recently found a procedure that might help to make life a little easier when faced with sectioning these critters. It involves using Butanol and ETOH. See if you can find a copy of "Manual of Basic Techniques in Insect Histology" by Pedro Barbosa. In it is a procedure called Stiles' N-Butyl Alcohol Technic. It is very time consuming - takes days but could be worth it. I had to order in the book from another library since the library here didn't have a copy and the book is out of print. Let me know if you want the details of this procedure and I can scan the pages I copied before returning the book and email them to you. Unfortunately I have not used this procedure to tell you myself how it works because the project was completed about the same time I found the book. Also - another method that works OK: the investigator who brought the last insect project to my lab fixed whiteflies in Penfix (Richard Allen Scientific). He removed the wings and legs and continued fixing for 36 hrs. Rinsed in 70% ETOH and removed any remaining wings and legs he missed the first time. A tedious task for something as small as whiteflies! Glad he was doing it!!! Can you just picture this guy sitting there looking through the dissecting microscope pulling off their little legs and wings? Sounds evil! I processed on a MVP tissue processor, 15 min in each station starting at 70% ETOH then 80%, 2-95%, 3-100. All at room temp and vacuum used in the last station of the 95% and 100& ETOH. Then on to Xylene x2 for 20-30 minutes at Room temp and vacuum and pressure in the #2 station. Paraffin - infiltration is real important for good sectioning - I used all 4 paraffins at 60 degrees C - 30 min. in the first one and an hour in the remaining stations with vacuum and pressure. I embedded the critters - about 30-50 per block in Paraplast. The sections were OK - some knife marks but for the most part the sections were very good. I cut 5 micron sections on non-chilled blocks with minimal soaking in RT water for in-situ hybridization. IF THERE IS ANYBODY OUT THERE PROCESSING AND SECTIONING INSECTS WHO MAY HAVE SOME BETTER METHODS PLEASE SHARE THEM!!! Good luck! Andi Grantham At 11:01 AM 6/7/2006 +0200, Dr. Elke Genersch wrote: >For in situ hybridization we need to obtain sections from the heads of >bees (not just brain!) and from whole mites. Has anyone experience with >fixation, embedding and sectioning of such material ? > >Elke Genersch, PhD >Elke Genersch, Ph.D. >Vice Director >Institute for Bee Research >Friedrich-Engels-Stra?e 32 >D - 16540 Hohen Neuendorf > >Tel.: +49 - (0)3303 - 293833 >Fax: +49 - (0)3303 - 293840 >e-mail: elke.genersch@rz.hu-berlin.de >homepage: www.honigbiene.de > >Associated Member of the Zentrum f?r Infektionsbiologie und Immunit?t = ZIBI >www.biologie.hu-berlin.de/~ZIBI/ > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From Karen.Heckford <@t> CHW.edu Wed Jun 7 10:54:52 2006 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Wed Jun 7 10:55:28 2006 Subject: [Histonet] VIP E300 Message-ID: Hi Everyone, I need your help in locating the Catalog number for the Carbon Filter in the Tissue Tek VIP E300 tissue processor. I must have misplaced it. I thought I ordered them through Cardinal Health? Cheers, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathololgy Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu From jqb7 <@t> cdc.gov Wed Jun 7 11:19:48 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/NCID/VR)) Date: Wed Jun 7 11:24:43 2006 Subject: [Histonet] VIP E300 Message-ID: Sakura sells them: Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Wednesday, June 07, 2006 11:55 AM To: Histonet (E-mail) Subject: [Histonet] VIP E300 Hi Everyone, I need your help in locating the Catalog number for the Carbon Filter in the Tissue Tek VIP E300 tissue processor. I must have misplaced it. I thought I ordered them through Cardinal Health? Cheers, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathololgy Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu -------------- next part -------------- Sakura - View Items
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4899 ACTIVATED CARBON CARTRIDGE,VIP™5, VIP™ E150/E300, 2/CS 1 CASE $102.00

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From rjbuesa <@t> yahoo.com Wed Jun 7 11:38:32 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 7 11:38:40 2006 Subject: [Histonet] Neutralisation of 10% formalin using ammonia In-Reply-To: Message-ID: <20060607163832.42021.qmail@web61214.mail.yahoo.com> Anne: We are in the "same boat"; I also used Neutralex, but my answer was regarding as to how to make sure formalin was neutralized and even using Neutralex I tested always before discarfding it. I agree Sakura is the bast!! Hope you are well. Ren? J. Anne Van Binsbergen wrote: hey Mike and Rene! 'camel queens' use Neutralex by Sakura - its the best!!! hope you are both well - in your respective corners of the Histonet!! ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Wed 2006/06/07 06:39 PM To: Mike Kirby; Histonet (E-mail) Subject: Re: [Histonet] Neutralisation of 10% formalin using ammonia Mr. M: Schiff's reagent is perhaps the best, most sensitive and available reagent in any lab to test for the presence of aldehydes. If you "neutralized" formalin turns purplish with the addition of Schiff's reagent, it is not totally neutralized. That was my reagent of choice when I tested different formalin neutralizing products. Sakura provides a kit containing Schiff's reagent to test their neutralizing product. Ren? J. Mike Kirby wrote: Dear Histonetters. The method of neutralising 10% formalin using concentrated ammonia solution has been well documented, and no doubt used by many Labs to dispose of their formalin waste. My first question is, how does one verify that all the formalin has been neutralised? One reference states that when the pH changes from 6 to 8, then sufficient ammonia has been added, and by implication, all the formalin should have been neutralised. My second question is, would Schiffs Reagent be of any use as a double check in this methodology, because as we all know, it turns pink, even at very low concentrations of formalin, or would the final product, Hexamethylenetetramine, also react with Schiffs? Has anyone pursued this avenue? Mr.M.Kirby Johannesburg South Africa. ********************************************************************************** The views expressed in this email are, unless otherwise stated, those of the author and not those of the National Health Laboratory Services or its management. The information in this e-mail is confidential and is intended solely for the addressee. Access to this e-mail by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken or omitted in reliance on this, is prohibited and may be unlawful. Whilst all reasonable steps are taken to ensure the accuracy and integrity of information and data transmitted electronically and to preserve the confidentiality thereof, no liability or responsibility whatsoever is accepted if information or data is, for whatever reason, corrupted or does not reach its intended destination. *********************************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From Linke_Noelle <@t> Allergan.com Wed Jun 7 11:39:04 2006 From: Linke_Noelle <@t> Allergan.com (Linke_Noelle) Date: Wed Jun 7 11:39:23 2006 Subject: [Histonet] job posting Message-ID: Hi all, We have an open position, please see below... Thanks! Noelle Linke, MS, HTL(ASCP)QIHC Allergan, Inc 2525 Dupont Drive RD-2A Irvine, CA 92612 714-246-5568 Associate Professional / Professional, Histology (in Pathology Department) Histology Technician/Technologist Primary responsibilities include collection and trimming of multiple organs, specimen processing, embedding, sectioning, staining, and coverslipping, in a GLP regulated research facility. Competency with PC based standard office software, such as MS-Word, EXCEL and PowerPoint desirable. The successful candidate will be well organized, methodical, detail oriented, self-motivated, focused, dependable, and take pride in their work. Position requires a BS and minimum 2 years of relevant experience (Technician) or HT/HTL ASCP certification (Technologist). * AA/EOE M/F/D/V For consideration, please apply online at http://www.allergan.com/site/careers/home.asp by entering "histology" into the search line and completing the application steps for the above-referenced position or by emailing Willard_ryan@allergan.com From beckerm <@t> labcorp.com Wed Jun 7 11:38:50 2006 From: beckerm <@t> labcorp.com (Michele Becker) Date: Wed Jun 7 11:39:50 2006 Subject: [Histonet] VIP E300 Message-ID: We order it through Cardinal Health, M7323-11. >>> "Bartlett, Jeanine (CDC/NCID/VR)" 06/07/06 12:19 PM >>> Sakura sells them: Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Wednesday, June 07, 2006 11:55 AM To: Histonet (E-mail) Subject: [Histonet] VIP E300 Hi Everyone, I need your help in locating the Catalog number for the Carbon Filter in the Tissue Tek VIP E300 tissue processor. I must have misplaced it. I thought I ordered them through Cardinal Health? Cheers, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathololgy Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu ----------------------------------------- This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer@labcorp.com or call (877) 23-HIPAA / (877) 234-4722. From elke.genersch <@t> rz.hu-berlin.de Wed Jun 7 12:15:46 2006 From: elke.genersch <@t> rz.hu-berlin.de (Dr. Elke Genersch) Date: Wed Jun 7 12:16:10 2006 Subject: [Histonet] sectioning bee's heads and mites In-Reply-To: <4.3.2.7.2.20060607081912.025b8a90@algranth.inbox.email.ari zona.edu> References: <5.1.0.14.1.20060607105605.02a312a8@popserv.rz.hu-berlin.de > Message-ID: <5.1.0.14.1.20060607190511.02b45e10@popserv.rz.hu-berlin.de> Mr. Grantham, unfortunately, I can't do paraffin sections. I have to use historesin as embedding media since the sectioning is done in another lab where only this embedding method is used (and liked). We found a paper (Brazil et al., 2003; comparison of different fixation and infiltration techniques) where sand fly specimens were fixed in Bouin/Carnoy and embedded in historesin with OK results - so they wrote. It did not work out as described with our bee heads and mites. If you could scan and pdf the pages from the "Manual of bacis techniques in insect histology" you refer to - or any other pages relating to my problem - it would be great. We don't have the book in the library. Is Penfix compatible with historesin? Elke Genersch At 08:53 07.06.2006 -0700, Andrea Grantham wrote: >Dr. Genersch, > From time to time I have to section insects and it is difficult since > their little bodies are hard on the outside and processing makes them > harder. I recently found a procedure that might help to make life a > little easier when faced with sectioning these critters. It involves > using Butanol and ETOH. See if you can find a copy of "Manual of Basic > Techniques in Insect Histology" by Pedro Barbosa. In it is a procedure > called Stiles' N-Butyl Alcohol Technic. It is very time consuming - takes > days but could be worth it. I had to order in the book from another > library since the library here didn't have a copy and the book is out of > print. Let me know if you want the details of this procedure and I can > scan the pages I copied before returning the book and email them to you. > Unfortunately I have not used this procedure to tell you myself how it > works because the project was completed about the same time I found the book. >Also - another method that works OK: the investigator who brought the last >insect project to my lab fixed whiteflies in Penfix (Richard Allen >Scientific). He removed the wings and legs and continued fixing for 36 >hrs. Rinsed in 70% ETOH and removed any remaining wings and legs he missed >the first time. A tedious task for something as small as whiteflies! Glad >he was doing it!!! Can you just picture this guy sitting there looking >through the dissecting microscope pulling off their little legs and wings? >Sounds evil! >I processed on a MVP tissue processor, 15 min in each station starting at >70% ETOH then 80%, 2-95%, 3-100. All at room temp and vacuum used in the >last station of the 95% and 100& ETOH. Then on to Xylene x2 for 20-30 >minutes at Room temp and vacuum and pressure in the #2 station. Paraffin - >infiltration is real important for good sectioning - I used all 4 >paraffins at 60 degrees C - 30 min. in the first one and an hour in the >remaining stations with vacuum and pressure. I embedded the critters - >about 30-50 per block in Paraplast. >The sections were OK - some knife marks but for the most part the sections >were very good. I cut 5 micron sections on non-chilled blocks with minimal >soaking in RT water for in-situ hybridization. >IF THERE IS ANYBODY OUT THERE PROCESSING AND SECTIONING INSECTS WHO MAY >HAVE SOME BETTER METHODS PLEASE SHARE THEM!!! >Good luck! >Andi Grantham > > >At 11:01 AM 6/7/2006 +0200, Dr. Elke Genersch wrote: >>For in situ hybridization we need to obtain sections from the heads of >>bees (not just brain!) and from whole mites. Has anyone experience with >>fixation, embedding and sectioning of such material ? >> >>Elke Genersch, PhD >>Elke Genersch, Ph.D. >>Vice Director >>Institute for Bee Research >>Friedrich-Engels-Stra?e 32 >>D - 16540 Hohen Neuendorf >> >>Tel.: +49 - (0)3303 - 293833 >>Fax: +49 - (0)3303 - 293840 >>e-mail: elke.genersch@rz.hu-berlin.de >>homepage: www.honigbiene.de >> >>Associated Member of the Zentrum f?r Infektionsbiologie und Immunit?t = ZIBI >>www.biologie.hu-berlin.de/~ZIBI/ >> >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >..................................................................... >: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : >: Sr. Research Specialist University of Arizona : >: (office: AHSC 4212) P.O. Box 245044 : >: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : >: (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : >:...................................................................: > http://www.cba.arizona.edu/histology-lab.html > From Karen.Heckford <@t> CHW.edu Wed Jun 7 12:30:21 2006 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Wed Jun 7 12:30:42 2006 Subject: [Histonet] Jones silver Message-ID: Does anyone know if a Jones' silver kit exist and where would I purchase it. Thanks again, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathololgy Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu From Charlene.Henry <@t> STJUDE.ORG Wed Jun 7 12:52:50 2006 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Wed Jun 7 12:52:57 2006 Subject: [Histonet] Jones silver. . Message-ID: <5CB39BCA5724F349BCB748675C6CA1A2099A1CB6@SJMEMXMB02.stjude.sjcrh.local> If you have a Ventana special stain module, their Jones silver kit works great. Charlene Henry HT (ASCP), QIHC Histology/Immunohistochemistry Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Wednesday, June 07, 2006 12:30 PM To: Histonet (E-mail) Subject: [Histonet] Jones silver. . Does anyone know if a Jones' silver kit exist and where would I purchase it. Thanks again, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathololgy Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu From cbass <@t> bidmc.harvard.edu Wed Jun 7 13:28:16 2006 From: cbass <@t> bidmc.harvard.edu (Caroline Bass) Date: Wed Jun 7 13:28:30 2006 Subject: [Histonet] dapi and GFP problem problem In-Reply-To: <6.0.0.22.1.20060607093827.01b499f8@gemini.msu.montana.edu> References: <03BAA0B6-AA0B-4CAD-9A99-99CEF1F0A916@bidmc.harvard.edu> <6.0.0.22.1.20060607093827.01b499f8@gemini.msu.montana.edu> Message-ID: <2C73F053-06D6-493C-A045-CB2F71D98CD9@bidmc.harvard.edu> If you mount the unfixed cells do you have any special considerations? In other words do you have to view them right away, will the slide last, etc? Thanks, Caroline On Jun 7, 2006, at 11:53 AM, Gayle Callis wrote: > You are probably experiencing autofluorescence induced the aldehyde > fixation. You can try Molecular Probes Image - IT image enhancer > that is supposed to get rid of the autofluorescence. > > It is important to note that Prolong Gold antifade is supposed to > sit overnight and not sealed with fingernail polish, by curing > overnight, this mounting media, the refractive index gradually > increses as it cures. This may be why the control appeared black > just after you mounted the coverslip. > > You can tell the CLSM to get rid of the autofluorescence too, some > might consider this cheating, but it can be done. What may be > better is to NOT fix the cells, and view them with CLSM - GFP is > designed for use with living cells and you may find the GFP is even > brighter. The Clontech manual called Living Colours tells you a > great deal about handling cells, etc with GFP. That manual can be > accessed online in pdf form. > > Unfixed cells can also be mounted with Prolong Gold antifade w/ > DAPI - we do this with unfixed frozen sections to avoid all > fixation or you can add a DAPI solution to the unfixed cells, and > view them without coverslipping or coverslipping with PBS. > > At 11:13 PM 6/6/2006, you wrote: > >> Hey, >> >> I have a GFP labeled g-protein coupled receptor that I am expressing >> in HEK 293 cells. I cultured the cells on collagen coated glass >> chamber slides, fixed the cells with 4% PFA, and then coverslipped >> with Prolong Gold antifade containing dapi. Before I coverslipped I >> checked the slide on a standard fluorescent microscope to make sure >> everything was expressing correctly. My transduced cells had a nice >> signal and my control was black. The day after coverslipping my >> control cells showed a noticeable green signal, not quite a bright as >> the GFP cells, but it is apparent. I took confocal pics and there >> was clearly some green in my control cells. >> >> Does anyone have a suggestions? I figure one of two things >> happened. Either some cells slid around when I pressed down on the >> coverslip or maybe I am getting some sort of bleedthrough. I am not >> very good with the confocal yet, so I don't know if I did something >> wrong here. Does anyone have a good protocol for growing 293 cells >> on glass slides? I have heard there of some super adherent clones of >> 293 that I would like to try, although I haven't found a source for >> them. >> >> Any and all suggestions would be appreciated. >> >> Thanks, >> >> Caroline >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 > 406 994-4303 (FAX) > > > From chapcl <@t> yahoo.com Wed Jun 7 13:28:31 2006 From: chapcl <@t> yahoo.com (William Chappell) Date: Wed Jun 7 13:28:38 2006 Subject: [Histonet] Need help ASAP in Los Angeles Message-ID: <20060607182831.46624.qmail@web37206.mail.mud.yahoo.com> My name is William Chappell, I am currently living in Los Angeles pursueing an acting career. I spent the last six years as a traveling histologist all over the country, but never in Los Angeles. A television project I am currently working on needs to film me working at a microtome in a laboratory setting. We need to find this lab today or tomorrow (June 7, or 8, 2006). If you can help or have any leads drop me an email. chapcl@yahoo.com. Thanks From chapcl <@t> yahoo.com Wed Jun 7 13:52:19 2006 From: chapcl <@t> yahoo.com (William Chappell) Date: Wed Jun 7 13:52:26 2006 Subject: [Histonet] Need help ASAP in Los Angeles Message-ID: <20060607185219.89626.qmail@web37215.mail.mud.yahoo.com> Note, the Laboratory must be in Los Angeles or Orange County due to time contstraints. Thanks again. From Eric.C.Kellar <@t> questdiagnostics.com Wed Jun 7 14:10:03 2006 From: Eric.C.Kellar <@t> questdiagnostics.com (Kellar, Eric C) Date: Wed Jun 7 14:10:44 2006 Subject: [Histonet] Jones silver Message-ID: <6843061CE6B98E4B96590D4F299618F801583BC4@qdcws0117.us.qdx.com> Karen, There is a manual Jone's silver basement membrane kit for kidney available in prefilled 8oz bottles from PolyScientific Cat # k025 1-800-645-5825. Works for me! Eric C. Kellar Quest Diagnostics, Inc Miami -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Heckford, Karen - SMMC-SF Sent: Wednesday, June 07, 2006 1:30 PM To: Histonet (E-mail) Subject: [Histonet] Jones silver Does anyone know if a Jones' silver kit exist and where would I purchase it. Thanks again, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathololgy Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu ============================================================================== The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. ============================================================================== From anh2006 <@t> med.cornell.edu Wed Jun 7 14:21:39 2006 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Wed Jun 7 14:20:56 2006 Subject: [Histonet] dapi and GFP problem problem In-Reply-To: <2C73F053-06D6-493C-A045-CB2F71D98CD9@bidmc.harvard.edu> References: <03BAA0B6-AA0B-4CAD-9A99-99CEF1F0A916@bidmc.harvard.edu> <6.0.0.22.1.20060607093827.01b499f8@gemini.msu.montana.edu> <2C73F053-06D6-493C-A045-CB2F71D98CD9@bidmc.harvard.edu> Message-ID: I really do not think fixation is the problem. Speaking from experience, for monolayers, aldehyde autofluorescence is RARELY a problem. And without fixation your cells are not going to look so great and in fact might look absolutely horrible!!!! I feel fairly confident that the sealing of the Prolong Gold with Permount did the damage. Andrea >If you mount the unfixed cells do you have any special >considerations? In other words do you have to view them right away, >will the slide last, etc? > >Thanks, > >Caroline > > >On Jun 7, 2006, at 11:53 AM, Gayle Callis wrote: > >>You are probably experiencing autofluorescence induced the aldehyde >>fixation. You can try Molecular Probes Image - IT image enhancer >>that is supposed to get rid of the autofluorescence. >> >>It is important to note that Prolong Gold antifade is supposed to >>sit overnight and not sealed with fingernail polish, by curing >>overnight, this mounting media, the refractive index gradually >>increses as it cures. This may be why the control appeared black >>just after you mounted the coverslip. >> >>You can tell the CLSM to get rid of the autofluorescence too, some >>might consider this cheating, but it can be done. What may be >>better is to NOT fix the cells, and view them with CLSM - GFP is >>designed for use with living cells and you may find the GFP is even >>brighter. The Clontech manual called Living Colours tells you a >>great deal about handling cells, etc with GFP. That manual can be >>accessed online in pdf form. >> >>Unfixed cells can also be mounted with Prolong Gold antifade w/ >>DAPI - we do this with unfixed frozen sections to avoid all >>fixation or you can add a DAPI solution to the unfixed cells, and >>view them without coverslipping or coverslipping with PBS. -- From ROrr <@t> enh.org Wed Jun 7 15:43:34 2006 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Wed Jun 7 15:43:43 2006 Subject: [Histonet] Overnight HIER Message-ID: Mark, I would imagine a lower temp would work, I haven't tried that. I have had great success with running overnight in a 60'C environment. The Biocare pressure cookers can be programmed to run at a stable temp overnight. The staining is generally very good and the morphology is just perfect with a citrate solution. Morphology isn't as good with overnight using a high pH. Hope this helps. Becky Orr CLA,HT(ASCP) Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 From algranth <@t> u.arizona.edu Wed Jun 7 16:23:13 2006 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Wed Jun 7 16:23:22 2006 Subject: [Histonet] Need help ASAP in Los Angeles In-Reply-To: <20060607185219.89626.qmail@web37215.mail.mud.yahoo.com> Message-ID: <4.3.2.7.2.20060607142136.025dedb8@algranth.inbox.email.arizona.edu> You realize, of course, that you will have to announce on histonet when and where this film will be available for our expert screening. Andi Grantham At 11:52 AM 6/7/2006 -0700, William Chappell wrote: >Note, the Laboratory must be in Los Angeles or Orange >County due to time contstraints. Thanks again. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From gcallis <@t> montana.edu Wed Jun 7 16:42:30 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Jun 7 16:42:46 2006 Subject: At the risk of being punny Re: [Histonet] Need help ASAP in Los Angeles In-Reply-To: <4.3.2.7.2.20060607142136.025dedb8@algranth.inbox.email.ari zona.edu> References: <20060607185219.89626.qmail@web37215.mail.mud.yahoo.com> <4.3.2.7.2.20060607142136.025dedb8@algranth.inbox.email.arizona.edu> Message-ID: <6.0.0.22.1.20060607153138.01b31320@gemini.msu.montana.edu> Maybe a new CSI series, i.e. CSI-Los Angeles where he can section by the light of a mini-flashlight in a darkened room. Or maybe we can call him "The Orange County Chopper" so he can ride to work on a custom motorcycle aka "chopper" and section i.e. "chop", er, I mean section those blocks. He said he has expertise, so I doubt the "c" word applies. Gayle Callis At 03:23 PM 6/7/2006, you wrote: >You realize, of course, that you will have to announce on histonet when >and where this film will be available for our expert screening. > >Andi Grantham > > > >At 11:52 AM 6/7/2006 -0700, William Chappell wrote: >>Note, the Laboratory must be in Los Angeles or Orange >>County due to time contstraints. Thanks again. >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >..................................................................... >: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : >: Sr. Research Specialist University of Arizona : >: (office: AHSC 4212) P.O. Box 245044 : >: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : >: (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : >:...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Wed Jun 7 17:12:48 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Wed Jun 7 17:13:26 2006 Subject: At the risk of being punny Re: [Histonet] Need help ASAP in Los Angeles References: <6.0.0.22.1.20060607153138.01b31320@gemini.msu.montana.edu> Message-ID: ...or the Chapell Show....... ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Gayle Callis Sent: Wed 6/7/2006 5:42 PM To: Andrea Grantham; Histonet@lists.utsouthwestern.edu Subject: At the risk of being punny Re: [Histonet] Need help ASAP in Los Angeles Maybe a new CSI series, i.e. CSI-Los Angeles where he can section by the light of a mini-flashlight in a darkened room. Or maybe we can call him "The Orange County Chopper" so he can ride to work on a custom motorcycle aka "chopper" and section i.e. "chop", er, I mean section those blocks. He said he has expertise, so I doubt the "c" word applies. Gayle Callis At 03:23 PM 6/7/2006, you wrote: >You realize, of course, that you will have to announce on histonet when >and where this film will be available for our expert screening. > >Andi Grantham > > > >At 11:52 AM 6/7/2006 -0700, William Chappell wrote: >>Note, the Laboratory must be in Los Angeles or Orange >>County due to time contstraints. Thanks again. >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >..................................................................... >: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : >: Sr. Research Specialist University of Arizona : >: (office: AHSC 4212) P.O. Box 245044 : >: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : >: (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : >:...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From fslovinsky <@t> yahoo.com Wed Jun 7 17:24:50 2006 From: fslovinsky <@t> yahoo.com (Frank Slovinsky) Date: Wed Jun 7 17:24:55 2006 Subject: [Histonet] Olympus BHT microscope Message-ID: <20060607222450.92038.qmail@web32906.mail.mud.yahoo.com> I have available an Olympus BHT microscope, in excellent condition. The scope has all the objectives and has a travel case. __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From sjt2104 <@t> columbia.edu Wed Jun 7 17:26:28 2006 From: sjt2104 <@t> columbia.edu (Sebastien Thuault) Date: Wed Jun 7 17:26:27 2006 Subject: [Histonet] Nissl stain and shrinking Message-ID: Hi, I am trying to develop a way to do nissl stains on PFA fixed brain tissue without having to use the dehydration (ethanol) and clearing steps (xylene) steps, just the cresyl violet step. Has anyone tried that before? Do you have any advise? Thanks! Sebastien Thuault, PhD Center for Neurobiology and Behavior Columbia University PI Annex/Kolb Institute 1051 Riverside Drive New York, NY 10032 Phone: +1-212-543-5037 Fax: +1-212-795-7997 From sjt2104 <@t> columbia.edu Wed Jun 7 17:37:25 2006 From: sjt2104 <@t> columbia.edu (Sebastien Thuault) Date: Wed Jun 7 17:37:25 2006 Subject: [Histonet] mounting very thick specimen Message-ID: Does anyone have tips to mount very thick specimen (400 um) on slides in aqueous or resinous medium? Thanks! Sebastien Thuault, PhD Center for Neurobiology and Behavior Columbia University PI Annex/Kolb Institute 1051 Riverside Drive New York, NY 10032 Phone: +1-212-543-5037 Fax: +1-212-795-7997 From sjt2104 <@t> columbia.edu Wed Jun 7 17:57:00 2006 From: sjt2104 <@t> columbia.edu (Sebastien Thuault) Date: Wed Jun 7 17:56:58 2006 Subject: [Histonet] neuronal recontruction with polar tracer/ tissue clearing with glycerol / shrinkage issue Message-ID: Hi, Does anyone have experience with glycerol clearing? I am trying to clear very thick slices of brain tissue fixed in PFA (400 um) but do not want to use ethanol + xylene as I find the procedure makes the tissue shrink. I inject biocytin in one or two neurones and then reaveal morphology with a streptavidin coupled to peroxydase + DAB/H2O2 procedure. When I air dry the tissue and use ethanol and xylene, the slice shrinks a lot and the neuron's dendrites tends to become very sinuous. I find that using glycerol (increasing concentration from 25% to 100% in15% increments, 60 min in each bath) just after the DAB step prevents the distortion but is not as good as xylene to clear the tissue and very time consuming. Does anyone know of an alternative to limit tissue distortion/shrinkage and/or clearing? I f someone using the same morphological reconstruction technique has experience with this problem or has a protocol that does not lead to tissue distortion please post! Thanks! Sebastien Thuault, PhD Center for Neurobiology and Behavior Columbia University PI Annex/Kolb Institute 1051 Riverside Drive New York, NY 10032 Phone: +1-212-543-5037 Fax: +1-212-795-7997 From liz <@t> premierlab.com Wed Jun 7 18:01:19 2006 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Jun 7 17:59:35 2006 Subject: [Histonet] mounting very thick specimen In-Reply-To: Message-ID: <000e01c68a86$49b00140$0300a8c0@Chlipala> We routinely embed whole rat corneas. We use the smallest coverslip possible and place a good bit of mounting media on the slide and then place the cornea on the slide into the mounting media, from there we put a bit more mounting media on top of the specimen and then coverslip. We use a carrage bolt (3/8 x 4) with 3 hex nuts 3/8-16 that we place on top of the coverslip to get the specimen to remain flat when drying, it looks funny and you need to be careful when you place the bolt on, but it does work. We needed to do this because the cornea is not completely flat and even though it was notched a bit of weight on the coverglass helped the specimen remain flat. You might not need the bolts, if your specimen is nice and flat, you just need the right amount of mounting media. The amount that you need is going to be dependent upon the mounting media that you use, since no mounting media is the same and the amount of resin in the media is going to be different, you just have to work out the number of drops of media that you will need so you don't get any air bubbles once the media drys. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebastien Thuault Sent: Wednesday, June 07, 2006 4:37 PM To: Histonet Subject: [Histonet] mounting very thick specimen Does anyone have tips to mount very thick specimen (400 um) on slides in aqueous or resinous medium? Thanks! Sebastien Thuault, PhD Center for Neurobiology and Behavior Columbia University PI Annex/Kolb Institute 1051 Riverside Drive New York, NY 10032 Phone: +1-212-543-5037 Fax: +1-212-795-7997 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1.1584 (20060607) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From jnocito <@t> satx.rr.com Wed Jun 7 19:34:34 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Jun 7 19:34:40 2006 Subject: [Histonet] Automation Ventana vs. Dako References: <1A9F2A6C5762524799A816F1F09744CF1E0AC5@SCREECH.ntcampus.smdc.org> Message-ID: <002801c68a93$50e4b7f0$0b69ce44@yourxhtr8hvc4p> I know there isn't one stop shopping for immunos. WE have 5-6 different companies we purchase from because, let's face it, I might get a better titer from company A than company B. Company B might not have the exact clone I need or whatever. What's frying my cookies is that I told my company to look at other alternatives before leaping. They didn't, so now they come to me to fix it. I can't fix this one. I just am tired of hearing them whine about the cost. Maybe they'll listen next time. Joe ----- Original Message ----- From: "Jasper, Thomas G." To: "Joe Nocito" Cc: Sent: Wednesday, June 07, 2006 10:10 AM Subject: RE: [Histonet] Automation Ventana vs. Dako Hey Joe, No flaming, what you're saying has validity. In thinking all this over it's never one size fits all (see my last post). I agree the pricing is high, but for us (and others probably) the "true cost" works out all right (for now anyway). Please don't get me wrong I think Dako is good stuff and other folks are solidly behind different automated systems. I'd like to believe these systems have their good points as well. And you know competition is good for everyone, so there you have it. Keep at it Joe, guys like you keep things honest and healthy. Tom J. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Joe Nocito Sent: Tuesday, June 06, 2006 6:30 PM To: Sebree Linda A.; Carla Frenchko; Heather Renko; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Automation Ventana vs. Dako flaming time. If the whole idea of a machine is to walk away, why would anyone apply the primary manually? That defeats the purpose of the machine. If I have to apply one reagent manually, might as well apply it all manually. As far as using the refillable dispensers, you can only do that a certain number of times before you; A-use a new dispenser or B- use the same dispenser, but replace the barcode label. Either way, you are adding an additional cost that isn't formulated into the equation when Ventana gives someone a cost/slide. I call that "creative accounting". No, I am not a fan of Ventana ( can you tell?), but I have several friends that work for Ventana. It's not the people, just the corporate mentality I can't digest. So you see, no matter how you slice it, Ventana's machines do not have the "ability" to even be a partially open system. I know I'll be hearing about this again. So, as not to be a coward, here's my signature block. Hell, I'll even add the telephone number so y'all won't have to look it up. Those of you know where I work, I'll be expecting a phone call either to me or my CEO. You know who you are. I've been told by my CEO before to back off. It hasn't worked before, it won't happen this time either. One other thing. As always, my opinions do not reflect the opinions of my employers and even some of my co-workers, lawyers, housekeeping, used car salepeople, etc. Commence the flaming. (Is it Friday yet?) Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX 210-892-3747 (that's my direct line) ----- Original Message ----- From: "Sebree Linda A." To: "Carla Frenchko" ; "Heather Renko" ; Sent: Tuesday, June 06, 2006 7:52 AM Subject: RE: [Histonet] Automation Ventana vs. Dako Misconception here: you can run antibodies from any company on Ventana instruments either by putting them in a user-fillable Ventana dispenser or by manually applying the antibody in a titration mode run. We do both of these as well as use some of Ventana's antibodies. The only thing you are "married to" is their detection chemistries, biotin block (if using) and amplification (if using). You can employ your own enzyme digestion reagent and counterstain if desired. If performing antigen retrieval on line, you also are wed to Ventana's Cell Conditioning solutions (HIER buffers)....although nothing prevents you from using your own HIER methods and reagents off-line which is what we do when using our NexES instrument. Just trying to clear a few things up. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Carla Frenchko Sent: Tuesday, June 06, 2006 6:53 AM To: Heather Renko; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Automation Ventana vs. Dako With all due respect to Heather; but the reason the Ventana is not really an "open" system is that you are "married" to a particular vendor's antibodies and detection. In a true "open" system most any company's antibodies will run with the instrument's detection (specifically speaking of both the Dako and Biogenex instruments having run both). Sincerely, Carla -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heather Renko Sent: Monday, June 05, 2006 10:22 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automation Ventana vs. Dako As an ex Ventana Tech Rep (not sales) I can say that you really have to judge what instrument is best suited for your volume and your staffing. Ventana is a great company with a great product but, very expensive. Can you rely on it-yes, Can any tech run it with minimal training-yes. Have they had lot issues with detection, sure but name a company that hasn't run into that at some point. Dako on the other hand is very reliable and has the market on good antibodies. Can anyone run it-not without a bit of hands training. It seems to be more affordable than Ventana but, you have to have a good staff that can consistently reproduce steps and make up dilutions. With Ventana you can cut, dry, put it on the instrument and then walk away-reproducible and consistent pretty much every time. With Ventana you can also create an open system and run some great Dako antibodies or any other vendors products for that fact. Your only marrried to their detection. Both companies seem to have good tech support and Dako has allot of really sharp publications to get you off and running. Unfortunately in today's histology labs we are seeing such an increase in staffing changes that it is sometimes better to pay the extra money and let the Ventana Benchmark do the work and not worry about it. On the other hand if you have a good tech staff that can be "cooks in the kitchen" than Dako seems to be a pretty good instrument too. I welcome you to listen to your peers-its just simply finding out which company will suit your needs and what your budget will endure. Just my un-bias two cents. I wish you all the best in your decison making process. Heather __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.8.2/357 - Release Date: 6/6/2006 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.8.2/357 - Release Date: 6/6/2006 From ree3 <@t> leicester.ac.uk Thu Jun 8 04:11:08 2006 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Thu Jun 8 04:11:21 2006 Subject: At the risk of being puny Re: [Histonet] Need help ASAP inLos Angeles Message-ID: or "Easy Chopper" -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Bonner, Janet Sent: 07 June 2006 23:13 To: Gayle Callis; Andrea Grantham; Histonet@lists.utsouthwestern.edu Subject: RE: At the risk of being punny Re: [Histonet] Need help ASAP inLos Angeles ...or the Chapell Show....... ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Gayle Callis Sent: Wed 6/7/2006 5:42 PM To: Andrea Grantham; Histonet@lists.utsouthwestern.edu Subject: At the risk of being punny Re: [Histonet] Need help ASAP in Los Angeles Maybe a new CSI series, i.e. CSI-Los Angeles where he can section by the light of a mini-flashlight in a darkened room. Or maybe we can call him "The Orange County Chopper" so he can ride to work on a custom motorcycle aka "chopper" and section i.e. "chop", er, I mean section those blocks. He said he has expertise, so I doubt the "c" word applies. Gayle Callis At 03:23 PM 6/7/2006, you wrote: >You realize, of course, that you will have to announce on histonet when >and where this film will be available for our expert screening. > >Andi Grantham > > > >At 11:52 AM 6/7/2006 -0700, William Chappell wrote: >>Note, the Laboratory must be in Los Angeles or Orange >>County due to time contstraints. Thanks again. >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >..................................................................... >: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : >: Sr. Research Specialist University of Arizona : >: (office: AHSC 4212) P.O. Box 245044 : >: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : >: (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : >:...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Thu Jun 8 04:36:59 2006 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Jun 8 04:37:36 2006 Subject: [Histonet] mounting very thick specimen In-Reply-To: Message-ID: <000601c68adf$17e4ab80$1d1c2643@HPPav2> Years ago, I had to mount whole liver flukes, which were thick, but not as thick as your 400 um (0.4 mm). Here's how we did it - see if it works for you: Take another glass slide, and carefully break it into little pieces, like 1-2 mm square pieces. (The safety officer in me had to say carefully, and please use safety goggles.) Take 4 little pieces, and place them flat around the outside of the tissue - far enough away so if the pieces move, they won't scratch the tissue, but close enough so that they would be located under the coverslip. Use mounting media, and put LOTS of drops of it on the slide and around the pieces of broken glass. Thicker viscosity (such as slightly old mounting media with some of the solvent evaporated out) works a little better, as it won't run all over the slide and the counter before it sets/dries. Coverslip by hand with a glass slide. Slow motion coverslipping helps, so the glass pieces don't move and bubble won't form. Since a lot of mounting media is used, it can take several days to set. Until set, the coverslip is very easy to move, and the mounting media will ooze out all over the slide, counter, and fingers. To speed up drying, place the coverslipped slide flat in a 60 degree oven for a couple of hours to overnight, to allow mounting media to solidify. Set the glass slide on top of a flat lid from a coplin jar, on the screwtop lip (the flat top is resting down on the shelf in the oven). That way, if any mounting media did escape and flow to the backside of the glass slide, the slide isn't "glued" to the oven shelf. (Learned that one the hard way.) Sometimes, not enough mounting media is used, and when dried, it has pulled inward away from the edge of the coverslip. Just add some more drops of mounting media on the slide, touching the edge of the coverslip. The drop(s) should wick into the empty space(s). Place back into the oven for a couple of hours to solidify. The coverslip is now being held up away from the tissue by the thickness of the pieces of broken glass. Let us know if this technique works for your project. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebastien Thuault Sent: Wednesday, June 07, 2006 6:37 PM To: Histonet Subject: [Histonet] mounting very thick specimen Does anyone have tips to mount very thick specimen (400 um) on slides in aqueous or resinous medium? Thanks! Sebastien Thuault, PhD Center for Neurobiology and Behavior Columbia University PI Annex/Kolb Institute 1051 Riverside Drive New York, NY 10032 Phone: +1-212-543-5037 Fax: +1-212-795-7997 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From louise.renton <@t> gmail.com Thu Jun 8 05:44:00 2006 From: louise.renton <@t> gmail.com (louise renton) Date: Thu Jun 8 05:44:04 2006 Subject: [Histonet] buffer rinses Message-ID: Hi all, How long and/or vigorous should buffer rinses between immunohisto steps be(ffpe 4um sections)? I am rinsing 5mins with agitation in PBS +0.05% Tween. Is this too long? I am concerned that I am washing away signal yet if I use PBS alone there is overwhelming background and general muckiness -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From stamptrain <@t> yahoo.com Thu Jun 8 06:43:56 2006 From: stamptrain <@t> yahoo.com (Roger Moretz) Date: Thu Jun 8 06:44:03 2006 Subject: At the risk of being puny Re: [Histonet] Need help ASAP inLos Angeles In-Reply-To: Message-ID: <20060608114356.83518.qmail@web50305.mail.yahoo.com> or "Chop Till You Drop" Roger Moretz --- "Edwards, R.E." wrote: > or "Easy Chopper" > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On > Behalf Of Bonner, > Janet > Sent: 07 June 2006 23:13 > To: Gayle Callis; Andrea Grantham; > Histonet@lists.utsouthwestern.edu > Subject: RE: At the risk of being punny Re: > [Histonet] Need help ASAP > inLos Angeles > > > ...or the Chapell Show....... > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on > behalf of Gayle Callis > Sent: Wed 6/7/2006 5:42 PM > To: Andrea Grantham; > Histonet@lists.utsouthwestern.edu > Subject: At the risk of being punny Re: [Histonet] > Need help ASAP in Los Angeles > > > > Maybe a new CSI series, i.e. CSI-Los Angeles where > he can section by the > light of a mini-flashlight in a darkened room. > > Or maybe we can call him "The Orange County Chopper" > so he can ride to work > on a custom motorcycle aka "chopper" and section > i.e. "chop", er, I mean > section those blocks. He said he has expertise, so > I doubt the "c" word > applies. > > Gayle Callis > > > > At 03:23 PM 6/7/2006, you wrote: > >You realize, of course, that you will have to > announce on histonet when > >and where this film will be available for our > expert screening. > > > >Andi Grantham > > > > > > > >At 11:52 AM 6/7/2006 -0700, William Chappell wrote: > > >>Note, the Laboratory must be in Los Angeles or > Orange > >>County due to time contstraints. Thanks again. > >> > >>_______________________________________________ > >>Histonet mailing list > >>Histonet@lists.utsouthwestern.edu > >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > >..................................................................... > > >: Andrea Grantham, HT(ASCP) Dept. of Cell > Biology & Anatomy : > >: Sr. Research Specialist University of > Arizona : > >: (office: AHSC 4212) P.O. Box 245044 > : > >: (voice: 520-626-4415) Tucson, AZ > 85724-5044 USA : > >: (FAX: 520-626-2097) (email: > algranth@u.arizona.edu) : > >:...................................................................: > > > > http://www.cba.arizona.edu/histology-lab.html > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From ree3 <@t> leicester.ac.uk Thu Jun 8 06:52:19 2006 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Thu Jun 8 06:52:28 2006 Subject: At the risk of being puny Re: [Histonet] Need help ASAP inLos Angeles Message-ID: or "Choppers R Us" -----Original Message----- From: Roger Moretz [mailto:stamptrain@yahoo.com] Sent: 08 June 2006 12:44 To: Edwards, R.E.; Bonner, Janet; Gayle Callis; Andrea Grantham; Histonet@lists.utsouthwestern.edu Subject: RE: At the risk of being puny Re: [Histonet] Need help ASAP inLos Angeles or "Chop Till You Drop" Roger Moretz --- "Edwards, R.E." wrote: > or "Easy Chopper" > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On > Behalf Of Bonner, > Janet > Sent: 07 June 2006 23:13 > To: Gayle Callis; Andrea Grantham; > Histonet@lists.utsouthwestern.edu > Subject: RE: At the risk of being punny Re: > [Histonet] Need help ASAP > inLos Angeles > > > ...or the Chapell Show....... > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on > behalf of Gayle Callis > Sent: Wed 6/7/2006 5:42 PM > To: Andrea Grantham; > Histonet@lists.utsouthwestern.edu > Subject: At the risk of being punny Re: [Histonet] > Need help ASAP in Los Angeles > > > > Maybe a new CSI series, i.e. CSI-Los Angeles where > he can section by the > light of a mini-flashlight in a darkened room. > > Or maybe we can call him "The Orange County Chopper" > so he can ride to work > on a custom motorcycle aka "chopper" and section > i.e. "chop", er, I mean > section those blocks. He said he has expertise, so > I doubt the "c" word > applies. > > Gayle Callis > > > > At 03:23 PM 6/7/2006, you wrote: > >You realize, of course, that you will have to > announce on histonet when > >and where this film will be available for our > expert screening. > > > >Andi Grantham > > > > > > > >At 11:52 AM 6/7/2006 -0700, William Chappell wrote: > > >>Note, the Laboratory must be in Los Angeles or > Orange > >>County due to time contstraints. Thanks again. > >> > >>_______________________________________________ > >>Histonet mailing list > >>Histonet@lists.utsouthwestern.edu > >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > >..................................................................... > > >: Andrea Grantham, HT(ASCP) Dept. of Cell > Biology & Anatomy : > >: Sr. Research Specialist University of > Arizona : > >: (office: AHSC 4212) P.O. Box 245044 > : > >: (voice: 520-626-4415) Tucson, AZ > 85724-5044 USA : > >: (FAX: 520-626-2097) (email: > algranth@u.arizona.edu) : > >:...................................................................: > > > > http://www.cba.arizona.edu/histology-lab.html > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From sohail_e <@t> yahoo.com Thu Jun 8 07:17:21 2006 From: sohail_e <@t> yahoo.com (sohail ejaz) Date: Thu Jun 8 07:17:31 2006 Subject: [Histonet] Computer software to decrease background staining in Immunoflouresence staining Message-ID: <20060608121721.83285.qmail@web30603.mail.mud.yahoo.com> Hi every body Is there any one who can recommend me any Computer software to decrease background staining in Immunoflouresence staining. Regards Dr.Ejaz __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From rjbuesa <@t> yahoo.com Thu Jun 8 07:27:36 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jun 8 07:27:40 2006 Subject: [Histonet] buffer rinses In-Reply-To: Message-ID: <20060608122736.29017.qmail@web61211.mail.yahoo.com> Louise: When I did IHC manually I used to rinse in the following way: In a glass container with FRESH (unused) PBS buffer, there was a slide holder; I removed the slides from the wet chamber after the given incubation period was completed and transferred the slides (one at a time) to the slides holders. Once all were there, I just moved the holder with the slides several times (5-7) and that was it. Took the slides out (one at a time), wipe them back the slides and around the tissue and added the following reagent. While incubating I discarded the used PBS and added fresh one for the next washing. Washing was OK without background and strong signal. If you want you could have 2 containers with PBS and after washing in the first could go to the second. Washing never took more than 1 minutes if 2 washing containers were used. Hope this will help you. Ren? J. louise renton wrote: Hi all, How long and/or vigorous should buffer rinses between immunohisto steps be(ffpe 4um sections)? I am rinsing 5mins with agitation in PBS +0.05% Tween. Is this too long? I am concerned that I am washing away signal yet if I use PBS alone there is overwhelming background and general muckiness -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From lesley.bechtold <@t> jax.org Thu Jun 8 07:36:12 2006 From: lesley.bechtold <@t> jax.org (Lesley Bechtold) Date: Thu Jun 8 07:37:53 2006 Subject: [Histonet] Shipping slides to customers Message-ID: <20060608083612746.00000007956@spikey> Hi Histonetters, I have a question for those of you who ship slides to customers; how do you ship them (cardboard mailers? plastic slide boxes?) and how do you charge your customers? Do you charge them separately for the boxes and mailers or do you include that as part of the shipping fee? Thank you! Lesley Lesley S. Bechtold Senior Manager, Histopathology & Microscopy Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 From yeepengtiang <@t> hotmail.com Thu Jun 8 07:45:47 2006 From: yeepengtiang <@t> hotmail.com (tiang yeepeng) Date: Thu Jun 8 07:45:52 2006 Subject: [Histonet] Computer software to decrease background staining in Immunoflouresence staining Message-ID: Hi Ejaz, I think a comment from Gayle Callis in "[Histonet] dapi and GFP problem problem" mentioned about the software for your problem :) With regards, TIANG YEE PENG Department of Medical Microbiology, Faculty of Medicine, University of Malaya. _________________________________________________________________ Express yourself instantly with MSN Messenger! Download today it's FREE! http://messenger.msn.click-url.com/go/onm00200471ave/direct/01/ From c.m.vanderloos <@t> amc.uva.nl Thu Jun 8 08:05:31 2006 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Thu Jun 8 08:05:42 2006 Subject: [Histonet] RE: HIER Message-ID: <9faade9fc795.9fc7959faade@amc.uva.nl> Dear Mark, I can echo Rebecca here: overnight HIER works fine with citrate pH6.0. Indeed, Tris-EDTA pH9.0 is a bit aggressive to the tissue morphology. We found overnight HIER extremely helpful with fatty tissues that are prone for floating. I would like to add here that one should not expect wonders from overnight HIER: the staining intensity for most antibodies is nearly the same as with "normal" HIER (15 min 100C). Of course we have an exception here (so far): CD3. That one stained much stronger after overnight HIER than with "normal" HIER. Lots of success! Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands References Visible links Hidden links: 1. mailto:ROrr@enh.org From mcauliff <@t> umdnj.edu Thu Jun 8 08:24:43 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Jun 8 08:25:12 2006 Subject: [Histonet] Computer software to decrease background staining in Immunoflouresence staining In-Reply-To: <20060608121721.83285.qmail@web30603.mail.mud.yahoo.com> References: <20060608121721.83285.qmail@web30603.mail.mud.yahoo.com> Message-ID: <4488251B.5040203@umdnj.edu> If you improve the quality of your immunostaining you won't need to do it with at computer. Removing/reducing background with a computer program could be considered fraud. Most journals can detect such manipulations and will reject your work on that basis alone. Geoff sohail ejaz wrote: >Hi every body > > Is there any one who can recommend me any Computer software to decrease background staining in Immunoflouresence staining. > > Regards > > Dr.Ejaz > > > > __________________________________________________ >Do You Yahoo!? >Tired of spam? Yahoo! Mail has the best spam protection around >http://mail.yahoo.com >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From sbreeden <@t> nmda.nmsu.edu Thu Jun 8 08:59:15 2006 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Thu Jun 8 08:59:22 2006 Subject: [Histonet] XYL Substitutes vs IHC Message-ID: Has anyone had difficulty with BVD/CWD IHC results when the tissues/slides are processed through a xylene substitute (specifically in this case Sub-X)? I'm getting weak counterstaining and I'm wondering if this could be the source of the problems; I've pretty much ruled out mechanical or astrological causes. Any help would be greatly appreciated. I only use the substitute because of air quality issues within the lab although I still prefer xylene all around! Thanks! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87108 From cfavara <@t> niaid.nih.gov Thu Jun 8 09:08:56 2006 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID) [E]) Date: Thu Jun 8 09:09:01 2006 Subject: [Histonet] XYL Substitutes vs IHC In-Reply-To: Message-ID: Question before comment, is it only the counterstain that is weak? I will be interested to hear what other opinions are. I have used a substitute for more years than I want to think about and the results are more than adequate. I do however think that overall stains are not as crisp and bright as with previously used more harmful chemicals. Could be true or could be just fond memories of when I was far less busy, stressed and a hell of a lot younger! Cheers to all, c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives -----Original Message----- From: Breeden, Sara [mailto:sbreeden@nmda.nmsu.edu] Sent: Thursday, June 08, 2006 6:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] XYL Substitutes vs IHC Has anyone had difficulty with BVD/CWD IHC results when the tissues/slides are processed through a xylene substitute (specifically in this case Sub-X)? I'm getting weak counterstaining and I'm wondering if this could be the source of the problems; I've pretty much ruled out mechanical or astrological causes. Any help would be greatly appreciated. I only use the substitute because of air quality issues within the lab although I still prefer xylene all around! Thanks! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87108 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Thu Jun 8 09:27:26 2006 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Jun 8 09:25:42 2006 Subject: [Histonet] Shipping slides to customers In-Reply-To: <20060608083612746.00000007956@spikey> Message-ID: <000801c68b07$aa7d2d00$0300a8c0@Chlipala> Lesley We ship the blocks back in cardboard boxes (small part boxes) that we get from reliable office supply. Slides are shipped back in plastic slide boxes, we do not charge for the cost of the slide boxes or block boxes it is covered in our overall costs for the histology, but a lot of contract labs out there charge for slide boxes, we charge for shipping and handling when we ship the slides and blocks back unless we get a fed ex acct number from the client and then we will use that. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lesley Bechtold Sent: Thursday, June 08, 2006 6:36 AM To: Histology Network Subject: [Histonet] Shipping slides to customers Hi Histonetters, I have a question for those of you who ship slides to customers; how do you ship them (cardboard mailers? plastic slide boxes?) and how do you charge your customers? Do you charge them separately for the boxes and mailers or do you include that as part of the shipping fee? Thank you! Lesley Lesley S. Bechtold Senior Manager, Histopathology & Microscopy Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1.1586 (20060608) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From PMonfils <@t> Lifespan.org Thu Jun 8 09:30:45 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Jun 8 09:30:56 2006 Subject: [Histonet] mounting very thick specimen Message-ID: <09C945920A6B654199F7A58A1D7D1FDE01717731@lsexch.lsmaster.lifespan.org> Many years ago I worked for a biological supply company, making slides for sale to educational institutions. I used two products for the mounting of whole small invertebrates like planaria, daphnia, hydra, mites, etc. Depending on the dimensions of your "very thick specimens", one of these might be useful. The first is depression slides, also called concavity slides. These are glass slides, a little thicker than normal, with a round concavity about a half inch wide and a millimeter deep in the center of the slide. You slightly overfill the concavity with mounting medium, place the stained, dehydrated, cleared specimen into the medium, and apply a coverslip larger than the concavity. The medium flows out to the edges of the coverslip, and the slide looks just like a typical coverslipped slide, since the edges of the coverslip lie flat against the slide. The other method employed solvent resistant plastic rings which came in various diameters and depths. I used these primarily for mounting larger specimens like fleas, mosquitos, drosophila, etc. The round rings were flat on the edges and the top and bottom surfaces, in other words the cross section of one side of the ring would be square. I would prepare slides in advance by dipping a ring into diluted mounting medium or diluted polyvinyl acetate, placing it on a standard slide, and allowing it to dry, cementing the ring to the slide. Then the ring could be filled with mounting medium, a specimen placed into the mounting medium, and a round coverslip the same diameter as the ring placed on top. From hej01 <@t> health.state.ny.us Thu Jun 8 09:44:40 2006 From: hej01 <@t> health.state.ny.us (Helen E Johnson) Date: Thu Jun 8 09:45:12 2006 Subject: [Histonet] 2006 New York State Licensure Law Message-ID: To New York State Histonetters: I would like to hear from you if you have any concerns or comments on the proposed regulations to implement the Clinical Laboratory Technology Practice Act. (http://www.op.nysed.gov/clp.htm) Helen Johnson (hej01@health.state.ny.us) From Charles.Embrey <@t> carle.com Thu Jun 8 10:01:25 2006 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Thu Jun 8 10:01:32 2006 Subject: [Histonet] 2006 New York State Licensure Law Message-ID: I am not in New York but I looked over the proposed legislation and was concerned that there is mention of, "Histological Procedures": ? 8601. Definition of the practice of clinical laboratory technology and clinical laboratory technology practitioner. "Clinical laboratory technology" means the performance of microbiological, virological, serological, chemical, immunohematological, hematological, biophysical, cytogenetical, cytological or histological procedures and examinations But no mention of Histology Technicians, Histologists, Histotechnologists or Pathologists' Assistants. The proposal specifically mentions Medical Technologists and Cytotechnologists. What happened to the other categories? Can we be legislated out of a job? Charles Embrey Jr., PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Helen E Johnson Sent: Thursday, June 08, 2006 9:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] 2006 New York State Licensure Law To New York State Histonetters: I would like to hear from you if you have any concerns or comments on the proposed regulations to implement the Clinical Laboratory Technology Practice Act. (http://www.op.nysed.gov/clp.htm) Helen Johnson (hej01@health.state.ny.us) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Eric <@t> ategra.com Thu Jun 8 10:36:26 2006 From: Eric <@t> ategra.com (Eric Dye (ext 223)) Date: Thu Jun 8 10:23:43 2006 Subject: [Histonet] Temporary Histology opening- Name your price! Message-ID: Hi Fellow-Histonetters - Are you still at Your Histology Lab ? Right now I have a Temporary opening in New Hampshire- Routine Histology, and Mohs...Mon-Fri, no weekend, no call. 13 - 26 weeks... Also below is an updated listing of Histology permanent and temporary jobs I have throughout the country. If you are interested in any of the Histology j o b s listed below or anywhere else - please call me at 800-466-9919 ext 223 or cell - 407-756-5507. Most Histo Tech jobs are full-time dayshift (Except where indicated below) Here are some of my Newest Histology Jobs: ------------------------------------------------------ 01. Coastal, Georgia (close to S.C.) - HistoTech - Perm - must be A S C P (HT or HTL) - dayshift 02. South Florida - temp - HistoTech 03. Florida, West Coast - temp & perm - Bench Histotech 04. Pennsylvania, Pittsburgh - perm - Bench HistoTech - days 05. Virginia/D.C. Beltway East - Fairfax Area - Perm, Histo Tech 07. New Hampshire - Histotech Thank you, Eric Dye-Sr Allied Healthcare Recruiter 800-466-9919 ext 223 or Cell - 407-756-5507 P.S.: Feel free to pass along this email and my phone number to anyone who you think might be interested. P.S.S.: The clients are currently interviewing - and the job will close soon - so if you are interested, please call me today at 1-800-466-9919 ext 229 --------------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing 800 466 9919 ext 223 - voice 407 671 6075 - fax eric@ategra.com To Learn More About Ategra: http://www.ategra.com ---------------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. ---------------------------------------------------------------- ---------------------------------------------------------------- Note: this message is intended for: Fellow-Histonetters at histonet@lists.utsouthwestern.edu ---------------------------------------------------------------- From gcallis <@t> montana.edu Thu Jun 8 10:27:30 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Jun 8 10:27:38 2006 Subject: [Histonet] Computer software to decrease background staining in Immunoflouresence staining In-Reply-To: <4488251B.5040203@umdnj.edu> References: <20060608121721.83285.qmail@web30603.mail.mud.yahoo.com> <4488251B.5040203@umdnj.edu> Message-ID: <6.0.0.22.1.20060608092551.01b95be8@gemini.msu.montana.edu> Geoff is absolutely correct!!! Gayle CAllis At 07:24 AM 6/8/2006, you wrote: >If you improve the quality of your immunostaining you won't need to do it >with at computer. Removing/reducing background with a computer program >could be considered fraud. Most journals can detect such manipulations and >will reject your work on that basis alone. > >Geoff > >sohail ejaz wrote: > >>Hi every body >> >> Is there any one who can recommend me any Computer software to decrease >> background staining in Immunoflouresence staining. >> >> Regards >> >> Dr.Ejaz >> From TJasper <@t> smdc.org Thu Jun 8 10:30:07 2006 From: TJasper <@t> smdc.org (Jasper, Thomas G.) Date: Thu Jun 8 10:30:43 2006 Subject: [Histonet] Ford Royer Message-ID: <1A9F2A6C5762524799A816F1F09744CF1E0AC6@SCREECH.ntcampus.smdc.org> Am looking for you Ford. Drop me an e-mail (phone number would be good to). I've got some questions for you. Thanks Thomas Jasper HT (ASCP) BAS Anatomic Pathology Supervisor SMDC Clinical Laboratory Duluth, MN tjasper@smdc.org This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From froyer <@t> bitstream.net Thu Jun 8 10:51:46 2006 From: froyer <@t> bitstream.net (Ford Royer) Date: Thu Jun 8 10:51:59 2006 Subject: [Histonet] Ford Royer In-Reply-To: <1A9F2A6C5762524799A816F1F09744CF1E0AC6@SCREECH.ntcampus.smdc.org> Message-ID: <004401c68b13$72b96530$7701a80a@Ford> Hi Tom, You cal reach me at the numbers/email listed below. Ford M. Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL: 612-839-1046 Phone: 763-542-8725 Fax: 763-546-4830 eMail: froyer@bitstream.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jasper, Thomas G. Sent: Thursday, June 08, 2006 10:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ford Royer Am looking for you Ford. Drop me an e-mail (phone number would be good to). I've got some questions for you. Thanks Thomas Jasper HT (ASCP) BAS Anatomic Pathology Supervisor SMDC Clinical Laboratory Duluth, MN tjasper@smdc.org This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SHARON.OSBORN <@t> SPCORP.COM Thu Jun 8 11:57:22 2006 From: SHARON.OSBORN <@t> SPCORP.COM (Osborn, Sharon) Date: Thu Jun 8 11:57:27 2006 Subject: [Histonet] : Jones Silver Kit Message-ID: <9A919A5D70313A4D9C56A025710874080C72D9@kenmsg40.us.schp.com> Karen, Contact Ron Hightower at American MasterTech, PO Box 2539, Lodi, Ca. Phone is: 1.800.860.4073. About this time last year, Ron worked with me to develop the Jones Slver Stain in Kit form for a very large special project I was doing. The kit number created was CUKTJON; this was for 500ml of the solutions; cost was $250.00. You may not need this amount. I was doing hundreds of slides. Let me know how this works out for you. sharon osborn DNAX, SP BioPharma Palo Alto, CA Message: 2 Date: Wed, 7 Jun 2006 10:30:21 -0700 From: "Heckford, Karen - SMMC-SF" Subject: [Histonet] Jones silver To: "Histonet (E-mail)" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Does anyone know if a Jones' silver kit exist and where would I purchase it. Thanks again, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathololgy Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From Lori.Karnes <@t> saskatoonhealthregion.ca Thu Jun 8 12:06:14 2006 From: Lori.Karnes <@t> saskatoonhealthregion.ca (Karnes, Lori SktnHR) Date: Thu Jun 8 12:06:38 2006 Subject: [Histonet] unsubscribe Message-ID: <7A83E83CB5B81A4A83F6A8268392B0C2848576@stampy.sktnhr.ca> Lori Karnes ART Tech III, Histology Laboratory Saskatoon Health Region Phone: (306) 655-8197 Email: lori.karnes@saskatoonhealthregion.ca From c.m.vanderloos <@t> amc.uva.nl Thu Jun 8 12:47:01 2006 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Thu Jun 8 12:47:13 2006 Subject: [Histonet] RE: Computer software to decrease background Message-ID: Dear all, There is software (+ hardware) indeed to separate autofluorescence from real fluorescence. Spectral imaging is the way to unmix signals based on their spectral characteristics. Autofluorescence has a slightly different spectral characteristic than "real" fluorescence and can therefore be nicely separated from each other. At the CRI website ([1]http://www.cri-inc.com/products/nuance.asp) you should be able to find some good examples of it. Sorry folks I work with that great system now for a couple of months for both brightfield and fluorescence, but I never considered it as being fraud....... NB: you will see it isn't "fraud" in NSH workshop #2: "Immunoenzymatic multiple staining methods - A practical overview and possibilities of multi-color imaging by spectral analysis" by Chris van der Loos and Richard Levenson. (Saturday September 9th, whole day) Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Thu, 08 Jun 2006 09:27:30 -0600 From: Gayle Callis Subject: Re: [Histonet] Computer software to decrease background staining in Immunoflouresence staining To: Geoff McAuliffe , Histonet@lists.utsouthwestern.edu, [2]Histonet@lists.utsouthwestern.edu Geoff is absolutely correct!!! Gayle CAllis At 07:24 AM 6/8/2006, you wrote: >If you improve the quality of your immunostaining you won't need to do it >with at computer. Removing/reducing background with a computer program >could be considered fraud. Most journals can detect such manipulations and >will reject your work on that basis alone. > >Geoff > >sohail ejaz wrote: > >>Hi every body >> >> Is there any one who can recommend me any Computer software to decrease >> backgroun! d stainin References Visible links 1. http://www.cri-inc.com/products/nuance.asp 2. mailto:Histonet@lists.utsouthwestern.edu Hidden links: 3. http://www.cri-inc.com/ From pruegg <@t> ihctech.net Thu Jun 8 13:03:49 2006 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Thu Jun 8 13:03:47 2006 Subject: [Histonet] brain tissue sections Message-ID: <200606081803.k58I3a9U017990@chip.viawest.net> We are having a terrible time with our ffpe 4 micron rat brain tissue sections falling off the slides, we are using silane coated slides, air drying on edge on a small fan, then heating on a slide warmer flat at 55dc for 1 hour to overnight. Please help. The sections are falling off during deparaffinizing thru xylene and alcohols, haven't even gotten to HIER and IHC yet. thanks for your help, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From DOOLEEO <@t> shands.ufl.edu Thu Jun 8 13:05:19 2006 From: DOOLEEO <@t> shands.ufl.edu (Elaine Dooley) Date: Thu Jun 8 13:05:59 2006 Subject: [Histonet] BKV background Message-ID: Dear Histonetters, In the last couple of weeks we have been getting nuclear staining on the edges of most of our kidney biopsies when we stain them for BKV. Our negative control is negative. (both the negative and the BKV slides are treated with a protease digestion and are blocked for endogenous biotin). The control tissue we have is a large piece of autopsy kidney positive for BKV. It does not have nuclear staining on the edges. In the past we never seen nuclear staining on the edges of our needle biopsies. The processing area says they have not changed anything in the processing of kidney needle biopsies. We use Chemicons BKV large T antigen antibody we have tried many different dilutions but so far we are still seeing the artifact. Has anyone out there had this problem? Elaine Dooley Shands Teaching Hospital 325-265-0111 ext. 72117 From NSEARCY <@t> swmail.sw.org Thu Jun 8 13:10:37 2006 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Thu Jun 8 13:11:00 2006 Subject: [Histonet] Warthin Starry Message-ID: I have a tech that's having problems with the Warthin-Starry. She believes its the citric acid. Can someone share their procedure (the simpler the better) and specifics on citric acid? I believe that she is getting a large amt. of precipitate when she mixes silver?? Thanks From cfavara <@t> niaid.nih.gov Thu Jun 8 13:30:39 2006 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID) [E]) Date: Thu Jun 8 13:30:48 2006 Subject: [Histonet] brain tissue sections In-Reply-To: <200606081803.k58I3a9U017990@chip.viawest.net> Message-ID: Patsy, Has anything changed with fixation or processing? You most likely have thought of this but it is the first thing that came to mind. c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives -----Original Message----- From: Patsy Ruegg [mailto:pruegg@ihctech.net] Sent: Thursday, June 08, 2006 11:04 AM To: histonet@pathology.swmed.edu Subject: [Histonet] brain tissue sections We are having a terrible time with our ffpe 4 micron rat brain tissue sections falling off the slides, we are using silane coated slides, air drying on edge on a small fan, then heating on a slide warmer flat at 55dc for 1 hour to overnight. Please help. The sections are falling off during deparaffinizing thru xylene and alcohols, haven't even gotten to HIER and IHC yet. thanks for your help, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ander093 <@t> tc.umn.edu Thu Jun 8 13:32:50 2006 From: ander093 <@t> tc.umn.edu (LuAnn Anderson) Date: Thu Jun 8 13:33:01 2006 Subject: [Histonet] Fwd: Re: brain tissue sections Message-ID: <6.2.3.4.0.20060608133140.02fd8350@ander093.email.umn.edu> > >Hi Patsy, >Try air drying overnight before the 55 dg warmer. I always air dry >overnight for specials and I never lose sections. Good luck. > >LuAnn Anderson HT(ASCP) >Neuropathology lab >University of Minnesota > > >At 01:01 PM 6/8/2006, you wrote: >>We are having a terrible time with our ffpe rat brain tissue >>sections falling off the slides, we are using silane coated slides, >>air drying on edge on a small fan, then heating on a slide warmer >>flat at 55dc for 1 hour to overnight. Please help. The sections >>are falling off during deparaffinizing thru xylene and alcohols, >>haven't even gotten to HIER and IHC yet. >>thanks for your help, >>Patsy >> >>Patsy Ruegg, HT(ASCP)QIHC >>IHCtech, LLC >>Fitzsimmons BioScience Park >>12635 Montview Blvd. Suite 215 >>Aurora, CO 80010 >>P-720-859-4060 >>F-720-859-4110 >>wk email pruegg@ihctech.net >>web site www.ihctech.net >> >> >>This email is confidential and intended solely for the use of the >>Person(s) ('the intended recipient') to whom it was addressed. Any >>views or opinions presented are solely those of the author. It may >>contain information that is privileged & confidential within the >>meaning of applicable law. Accordingly any dissemination, >>distribution, copying, or other use of this message, or any of its >>contents, by any person other than the intended recipient may >>constitute a breach of civil or criminal law and is strictly >>prohibited. If you are NOT the intended recipient please contact >>the sender and dispose of this e-mail as soon as possible. >> >> >>--~--~---------~--~----~------------~-------~--~----~ >>You received this message because you are subscribed to the Google >>Groups "ihcrg" group. >>To post to this group, send email to ihcrg@googlegroups.com >>To unsubscribe from this group, send email to >>ihcrg-unsubscribe@googlegroups.com >>For more options, visit this group at http://groups.google.com/group/ihcrg >>-~----------~----~----~----~------~----~------~--~--- From cwscouten <@t> myneurolab.com Thu Jun 8 13:38:58 2006 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Thu Jun 8 13:39:32 2006 Subject: [Histonet] brain tissue sections Message-ID: <5784D843593D874C93E9BADCB87342AB0130709E@tpiserver03.Coretech-holdings.com> I used silane to coat glass pipettes so proteins would not stick. You seem to be using it as the glue for proteins. I would look into what silane does and is for if I were you. It is a silicone compound that can coat glass with a silicone layer. Nothing sticks well to silicone. Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg Sent: Thursday, June 08, 2006 1:04 PM To: histonet@pathology.swmed.edu Subject: [Histonet] brain tissue sections We are having a terrible time with our ffpe 4 micron rat brain tissue sections falling off the slides, we are using silane coated slides, air drying on edge on a small fan, then heating on a slide warmer flat at 55dc for 1 hour to overnight. Please help. The sections are falling off during deparaffinizing thru xylene and alcohols, haven't even gotten to HIER and IHC yet. thanks for your help, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cfavara <@t> niaid.nih.gov Thu Jun 8 13:51:30 2006 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID) [E]) Date: Thu Jun 8 13:51:37 2006 Subject: [Histonet] BKV background In-Reply-To: Message-ID: This is interesting to me as I was having a similar problem with an antibody that I use frequently on murine brain and had cytoplasmic staining in neurons but only on the outer edge of the tissue and the outer portion of the brain [away from the midline]. I took different blocks of brain tissue, same strain, same age but midline and did not get the staining. I did not notice this staining of neurons when I initially used this block. My simple thinking is that is may be what I am calling an edge effect of fixation and or processing. Most outer portion of tissue is exposed to fix and processing reagents for a bit longer may result over processed tissue. In my experience over processed tissue is more likely to have problems with artifacts. I am sure someone will have a different explanation! c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives -----Original Message----- From: Elaine Dooley [mailto:DOOLEEO@shands.ufl.edu] Sent: Thursday, June 08, 2006 11:05 AM To: Histonet@pathology.swmed.edu Subject: [Histonet] BKV background Dear Histonetters, In the last couple of weeks we have been getting nuclear staining on the edges of most of our kidney biopsies when we stain them for BKV. Our negative control is negative. (both the negative and the BKV slides are treated with a protease digestion and are blocked for endogenous biotin). The control tissue we have is a large piece of autopsy kidney positive for BKV. It does not have nuclear staining on the edges. In the past we never seen nuclear staining on the edges of our needle biopsies. The processing area says they have not changed anything in the processing of kidney needle biopsies. We use Chemicons BKV large T antigen antibody we have tried many different dilutions but so far we are still seeing the artifact. Has anyone out there had this problem? Elaine Dooley Shands Teaching Hospital 325-265-0111 ext. 72117 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nsnwl <@t> neuro.hfh.edu Thu Jun 8 13:52:48 2006 From: nsnwl <@t> neuro.hfh.edu (Nancy Lemke) Date: Thu Jun 8 13:53:04 2006 Subject: [Histonet] brain tissue sections Message-ID: <794b74e37b3096bd1c6b6a81832a3c4a@neuro.hfh.edu> Hi Patsy, The problem you describe is because the sections have not dried enough. LuAnn is exactly correct about air-drying over night. If you don't air-dry, the sections can look like they have exploded off of the slide as soon as you hit the xylene. This tip was given to me many eons ago by a technical specialist at Miles in Elkhart, Ind. He worked with VIP processors and had encountered this problem with one of his customers. After trying everything, they accidentally left the sections in a rack overnight and the problem was gone! I have blessed him many times over the years for saving the remaining shreds of my sanity. It does not matter if you use plain glass, gel-coated or charged slides. Rodent brains need the extra room temperature drying and I even do the same with human samples because I believe in superstitious behavior! Nancy Lemke Research Coordinator Hermelin Brain Tumor Center Henry Ford Hospital Detroit -----Original message----- From: "Patsy Ruegg" pruegg@ihctech.net Date: Thu, 08 Jun 2006 14:04:26 -0400 To: histonet@pathology.swmed.edu Subject: [Histonet] brain tissue sections > We are having a terrible time with our ffpe 4 micron rat brain tissue > sections falling off the slides, we are using silane coated slides, air > drying on edge on a small fan, then heating on a slide warmer flat at 55dc > for 1 hour to overnight. Please help. The sections are falling off during > deparaffinizing thru xylene and alcohols, haven't even gotten to HIER and > IHC yet. > thanks for your help, > Patsy > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech, LLC > Fitzsimmons BioScience Park > 12635 Montview Blvd. Suite 215 > Aurora, CO 80010 > P-720-859-4060 > F-720-859-4110 > wk email pruegg@ihctech.net > web site www.ihctech.net > > > This email is confidential and intended solely for the use of the Person(s) > ('the intended recipient') to whom it was addressed. Any views or opinions > presented are solely those of the author. It may contain information that is > privileged & confidential within the meaning of applicable law. Accordingly > any dissemination, distribution, copying, or other use of this message, or > any of its contents, by any person other than the intended recipient may > constitute a breach of civil or criminal law and is strictly prohibited. If > you are NOT the intended recipient please contact the sender and dispose of > this e-mail as soon as possible. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ============================================================================== Go to http://henryford.com We're Henry Ford. We Can. HFHS CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. 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If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================== From pruegg <@t> ihctech.net Thu Jun 8 15:20:03 2006 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Thu Jun 8 15:20:08 2006 Subject: [Histonet] brain tissue sections In-Reply-To: <794b74e37b3096bd1c6b6a81832a3c4a@neuro.hfh.edu> Message-ID: <200606082019.k58KJo9U021509@chip.viawest.net> Thanks everybody for the help with the rat brains falling off, I am not having this problem with any other tissue except the brains, when I say silane coated slides I mean plus charged slides (APES) I buy commercially from several vendors, I was under the impression that all plus charged slides were charged by coating with silane, so to me silane coated slides and plus charged slides were the same thing????? I cut sections at 4 microns and airdried for only 20 min before heating at 55dc. The consenses seems to be to airdry overnight before heating the next day and I am trying that today. Thanks again, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: Nancy Lemke [mailto:nsnwl@neuro.hfh.edu] Sent: Thursday, June 08, 2006 11:53 AM To: Patsy Ruegg; histonet@pathology.swmed.edu Subject: Re: [Histonet] brain tissue sections Hi Patsy, The problem you describe is because the sections have not dried enough. LuAnn is exactly correct about air-drying over night. If you don't air-dry, the sections can look like they have exploded off of the slide as soon as you hit the xylene. This tip was given to me many eons ago by a technical specialist at Miles in Elkhart, Ind. He worked with VIP processors and had encountered this problem with one of his customers. After trying everything, they accidentally left the sections in a rack overnight and the problem was gone! I have blessed him many times over the years for saving the remaining shreds of my sanity. It does not matter if you use plain glass, gel-coated or charged slides. Rodent brains need the extra room temperature drying and I even do the same with human samples because I believe in superstitious behavior! Nancy Lemke Research Coordinator Hermelin Brain Tumor Center Henry Ford Hospital Detroit -----Original message----- From: "Patsy Ruegg" pruegg@ihctech.net Date: Thu, 08 Jun 2006 14:04:26 -0400 To: histonet@pathology.swmed.edu Subject: [Histonet] brain tissue sections > We are having a terrible time with our ffpe 4 micron rat brain tissue > sections falling off the slides, we are using silane coated slides, > air drying on edge on a small fan, then heating on a slide warmer flat > at 55dc for 1 hour to overnight. Please help. The sections are > falling off during deparaffinizing thru xylene and alcohols, haven't > even gotten to HIER and IHC yet. > thanks for your help, > Patsy > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech, LLC > Fitzsimmons BioScience Park > 12635 Montview Blvd. Suite 215 > Aurora, CO 80010 > P-720-859-4060 > F-720-859-4110 > wk email pruegg@ihctech.net > web site www.ihctech.net > > > This email is confidential and intended solely for the use of the > Person(s) ('the intended recipient') to whom it was addressed. Any > views or opinions presented are solely those of the author. It may > contain information that is privileged & confidential within the > meaning of applicable law. Accordingly any dissemination, > distribution, copying, or other use of this message, or any of its > contents, by any person other than the intended recipient may > constitute a breach of civil or criminal law and is strictly > prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ============================================================================ == Go to http://henryford.com We're Henry Ford. We Can. HFHS CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. 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If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. ============================================================================ == From sjchtascp <@t> yahoo.com Thu Jun 8 15:27:36 2006 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Thu Jun 8 15:27:39 2006 Subject: [Histonet] muscle cross section Message-ID: <20060608202736.64493.qmail@web38214.mail.mud.yahoo.com> I'm having to process and embed rodent cross section muscle. Allot of the muscle is very small and irregular pieces when I recieve it for processing. By the time I'm ready to embed it, trying to find the cross section is almost anybodies guess. Lately I've been taking shallow cuts and staining them to make sure I have a fairly exceptable cross section. Makes for allot of extra work and sometime lost tissue. Has anyone any experiance and advice for this delemma. Thanks, steve __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From Rcartun <@t> harthosp.org Thu Jun 8 15:44:45 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Jun 8 15:45:38 2006 Subject: [Histonet] Diminishing support services Message-ID: <448853FD0200007700000626@hcnwgwds01.hh.chs> Are there any other hospitals out there where the pathologists accession cases and type their own reports? Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From HornHV <@t> archildrens.org Thu Jun 8 15:50:24 2006 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Jun 8 15:51:14 2006 Subject: [Histonet] Diminishing support services Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6BFA@EMAIL.archildrens.org> Uh....is this a joke??? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, June 08, 2006 3:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Diminishing support services Are there any other hospitals out there where the pathologists accession cases and type their own reports? Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From tpmorken <@t> labvision.com Thu Jun 8 15:57:54 2006 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Thu Jun 8 15:58:30 2006 Subject: [Histonet] Diminishing support services Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA20328D9AB@usca0082k08.labvision.apogent.com> Pathologists know how to type? Better not let that get out! Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, June 08, 2006 1:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Diminishing support services Are there any other hospitals out there where the pathologists accession cases and type their own reports? Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From John.Thweatt <@t> va.gov Thu Jun 8 15:58:59 2006 From: John.Thweatt <@t> va.gov (Thweatt, John T) Date: Thu Jun 8 15:59:07 2006 Subject: [Histonet] Farewell,Signing Off/Unsubscribe Message-ID: <631842D8371C4143A8C422186903F54628E9E9@VHAV18MSGA1.v18.med.va.gov> Dear All, The time has come for me to leave and sign off as I approach my final day here at the VA; but only for a little while as I hope to resume my same Histology duties in Washington in the very near future. So long for now. It is so good to hear from each of you as each one of you have so much to offer and share. I have learned something from each of you. It has been my pleasure. Sincerely, ************************************ Tom Thweatt, HT (ASCP) Amarillo VA Health Care System Pathology and Laboratory Medicine Service Anatomical Pathology 6010 Amarillo Boulevard West Amarillo, TX 79106 806-355-9703 x 7071 fax 806-354-7865 From Jackie.O'Connor <@t> abbott.com Thu Jun 8 16:03:24 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Jun 8 16:03:56 2006 Subject: [Histonet] Diminishing support services In-Reply-To: <448853FD0200007700000626@hcnwgwds01.hh.chs> Message-ID: What have you there? A 25 bed hospital? I didn't know pathologists could type . . . .:-)....... "Richard Cartun" Sent by: histonet-bounces@lists.utsouthwestern.edu 06/08/2006 03:44 PM To cc Subject [Histonet] Diminishing support services Are there any other hospitals out there where the pathologists accession cases and type their own reports? Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bwhitaker <@t> brownpathology.com Thu Jun 8 16:05:49 2006 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Thu Jun 8 16:03:57 2006 Subject: [Histonet] microarray instrumentation Message-ID: <000001c68b3f$517dfe90$3601a8c0@brownpathology.net> Hi Everyone, I'd like to hear any pros or cons about specific microarray instrumentation from users. Thanks! Bonnie Whitaker Lab Manager Brown & Associates Medical Laboratories 8076 El Rio Houston, Texas 77054 713-741-6677 From PMonfils <@t> Lifespan.org Thu Jun 8 16:06:58 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Jun 8 16:07:06 2006 Subject: [Histonet] muscle cross section Message-ID: <09C945920A6B654199F7A58A1D7D1FDE01717734@lsexch.lsmaster.lifespan.org> It may be easier to identify the direction the fibers run before processing the specimens. Place the specimen on a piece of filter paper under a dissecting scope. Roll it gently so that excess liquid is absorbed by the filter paper. If it's too wet, reflections from the liquid make it harder to see clearly. With small specimens of course you have to work fairly quickly to ensure that they don't dry too much. Once you establish the correct orientation, apply a tiny dot of india ink to the end of the specimen that will be on top at the moment of embedding - in other words, the end that will not be sectioned. Then just drop it back into the fixative. The tip of a sharp probe or needle of some kind can deliver a tiny dot of ink. Not a hypodermic needle though - that will draw up extra ink by capillary action, and may dispense more than you want. After processing, the ink will still be there, and you can orient the specimens by that mark even though you can't actually see the orientation directly. Also, not to state the obvious, perhaps you could speak to the persons preparing the specimens and ask them if it is possible to cut them so that they are longer than they are wide - in other words, so that the muscle fibers run parallel to the long axis of the specimen. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > Steven Coakley > Sent: Thursday, June 8, 2006 1:27 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] muscle cross section > > I'm having to process and embed rodent cross section muscle. Allot of the > muscle is very small and irregular pieces when I recieve it for > processing. By the time I'm ready to embed it, trying to find the cross > section is almost anybodies guess. Lately I've been taking shallow cuts > and staining them to make sure I have a fairly exceptable cross section. > Makes for allot of extra work and sometime lost tissue. Has anyone any > experiance and advice for this delemma. > > Thanks, > > steve > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Rcartun <@t> harthosp.org Thu Jun 8 16:07:00 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Jun 8 16:07:48 2006 Subject: [Histonet] Diminishing support services In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6BFA@EMAIL.archildrens.org> References: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6BFA@EMAIL.archildrens.org> Message-ID: <44885934020000770000062A@hcnwgwds01.hh.chs> Unfortunately, no. We have so little support in our accessioning/transcription area that some of us are accessioning our own consult cases and many of us are typing our own pathology reports. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Horn, Hazel V" 06/08/06 4:50 PM >>> Uh....is this a joke??? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, June 08, 2006 3:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Diminishing support services Are there any other hospitals out there where the pathologists accession cases and type their own reports? Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From JWEEMS <@t> sjha.org Thu Jun 8 16:12:11 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Thu Jun 8 16:11:34 2006 Subject: [Histonet] Diminishing support services Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD320295FE91@sjhaexc02.sjha.org> We have a part time pathologist who chooses to do his own reports. He does not accession at his regular job, but he has templates for everything - plugs those in and makes a few modifications as needed. He says it takes less time and is more efficient for him. j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard Cartun Sent: Thursday, June 08, 2006 5:07 PM To: Hazel V Horn; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Diminishing support services Unfortunately, no. We have so little support in our accessioning/transcription area that some of us are accessioning our own consult cases and many of us are typing our own pathology reports. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Horn, Hazel V" 06/08/06 4:50 PM >>> Uh....is this a joke??? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, June 08, 2006 3:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Diminishing support services Are there any other hospitals out there where the pathologists accession cases and type their own reports? Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From KParker <@t> mail.nih.gov Thu Jun 8 16:18:58 2006 From: KParker <@t> mail.nih.gov (Tuttle, Kimberly (NIH/NCI) [E]) Date: Thu Jun 8 16:19:07 2006 Subject: [Histonet] Diminishing support services In-Reply-To: <44885934020000770000062A@hcnwgwds01.hh.chs> Message-ID: Is there an issue with hiring more staff? Years ago, I worked in a clinical Lab where I was the only tech, I was able to get a few red cross volunteers for accessioning, filing ...etc. I have two summer students at the moment. -----Original Message----- From: Richard Cartun [mailto:Rcartun@harthosp.org] Sent: Thursday, June 08, 2006 4:07 PM To: Hazel V Horn; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Diminishing support services Unfortunately, no. We have so little support in our accessioning/transcription area that some of us are accessioning our own consult cases and many of us are typing our own pathology reports. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Horn, Hazel V" 06/08/06 4:50 PM >>> Uh....is this a joke??? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, June 08, 2006 3:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Diminishing support services Are there any other hospitals out there where the pathologists accession cases and type their own reports? Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ ------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Thu Jun 8 16:20:10 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Jun 8 16:22:13 2006 Subject: [Histonet] Diminishing support services In-Reply-To: References: <448853FD0200007700000626@hcnwgwds01.hh.chs> Message-ID: <44885C4A020000770000062F@hcnwgwds01.hh.chs> No, 850 bed, tertiary care facility. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Jackie M O'Connor" 06/08/06 5:03 PM >>> What have you there? A 25 bed hospital? I didn't know pathologists could type . . . .:-)....... "Richard Cartun" Sent by: histonet-bounces@lists.utsouthwestern.edu 06/08/2006 03:44 PM To cc Subject [Histonet] Diminishing support services Are there any other hospitals out there where the pathologists accession cases and type their own reports? Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From POWELL_SA <@t> Mercer.edu Thu Jun 8 16:26:01 2006 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Thu Jun 8 16:26:37 2006 Subject: [Histonet] Diminishing support services In-Reply-To: <44885934020000770000062A@hcnwgwds01.hh.chs> Message-ID: <01M3E288IDTM8WXJDW@Macon2.Mercer.edu> Some transcription can be done by voice recognition software. Although some of the medical terms may confuse the issue. A couple of my doctors use it when they are doing exams, seems to work for them. Also you may find that cross training the techs is an option too. I typed pathology reports after getting the slides out way back when life was much simpler. :) Of course you should pay them the transcriptionist salary as well. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, June 08, 2006 4:07 PM To: Hazel V Horn; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Diminishing support services Unfortunately, no. We have so little support in our accessioning/transcription area that some of us are accessioning our own consult cases and many of us are typing our own pathology reports. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Horn, Hazel V" 06/08/06 4:50 PM >>> Uh....is this a joke??? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, June 08, 2006 3:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Diminishing support services Are there any other hospitals out there where the pathologists accession cases and type their own reports? Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------------- -- The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================ == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bills <@t> icpmr.wsahs.nsw.gov.au Thu Jun 8 16:35:41 2006 From: bills <@t> icpmr.wsahs.nsw.gov.au (Bill Sinai) Date: Thu Jun 8 16:36:20 2006 Subject: [Histonet] Diminishing support services In-Reply-To: <44885C4A020000770000062F@wsahs.nsw.gov.au> Message-ID: <001701c68b43$7dafa2d0$0ecd080a@wsahs.nsw.gov.au> Bill Sinai Chief Hospital Scientist Tissue Pathology ICPMR Westmead Hospital Westmead NSW 2145 Phone 02 9845 7774 Fax 02 9687 2330 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, 9 June 2006 7:20 AM To: Jackie M O'Connor; histonet@lists.utsouthwestern.edu; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] Diminishing support services No, 850 bed, tertiary care facility. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Jackie M O'Connor" 06/08/06 5:03 PM >>> What have you there? A 25 bed hospital? I didn't know pathologists could type . . . .:-)....... "Richard Cartun" Sent by: histonet-bounces@lists.utsouthwestern.edu 06/08/2006 03:44 PM To cc Subject [Histonet] Diminishing support services Are there any other hospitals out there where the pathologists accession cases and type their own reports? Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. 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From nienhuis <@t> ucla.edu Thu Jun 8 16:55:13 2006 From: nienhuis <@t> ucla.edu (nienhuis@ucla.edu) Date: Thu Jun 8 16:55:20 2006 Subject: [Histonet] RE: Computer software to decrease background In-Reply-To: References: Message-ID: <20060608145513.1lriumdw8c4w8csc@mail.ucla.edu> There is also software that reduces background scatter from florescence that radiates 360 degrees from the object. Autoquant AutoDeblur has been used to good effect even on confocal images. $$$! Bob Nienhuis Quoting "C.M. van der Loos" : > > Dear all, > > There is software (+ hardware) indeed to separate autofluorescence > from real fluorescence. Spectral imaging is the way to unmix signals > based on their spectral characteristics. Autofluorescence has a > slightly different spectral characteristic than "real" fluorescence > and can therefore be nicely separated from each other. At the CRI > website ([1]http://www.cri-inc.com/products/nuance.asp) you should be > able to find some good examples of it. Sorry folks I work with that > great system now for a couple of months for both brightfield and > fluorescence, but I never considered it as being fraud....... > > NB: you will see it isn't "fraud" in NSH workshop #2: "Immunoenzymatic > multiple staining methods - A practical overview and possibilities of > multi-color imaging by spectral analysis" by Chris van der Loos and > Richard Levenson. (Saturday September 9th, whole day) > > Chris van der Loos, PhD > Dept. of Pathology > Academic Medical Center M2-230 > Meibergdreef 9 > NL-1105 AZ Amsterdam > The Netherlands > > Date: Thu, 08 Jun 2006 09:27:30 -0600 > From: Gayle Callis > Subject: Re: [Histonet] Computer software to decrease background > staining in Immunoflouresence staining > To: Geoff McAuliffe , > Histonet@lists.utsouthwestern.edu, > [2]Histonet@lists.utsouthwestern.edu > Geoff is absolutely correct!!! > Gayle CAllis > At 07:24 AM 6/8/2006, you wrote: > >If you improve the quality of your immunostaining you won't need to > do it > >with at computer. Removing/reducing background with a computer > program > >could be considered fraud. Most journals can detect such > manipulations and > >will reject your work on that basis alone. > > > >Geoff > > > >sohail ejaz wrote: > > > >>Hi every body > >> > >> Is there any one who can recommend me any Computer software to > decrease > >> backgroun! d stainin > > References > > Visible links > 1. http://www.cri-inc.com/products/nuance.asp > 2. mailto:Histonet@lists.utsouthwestern.edu > > Hidden links: > 3. http://www.cri-inc.com/ > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Rcartun <@t> harthosp.org Thu Jun 8 17:15:26 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Jun 8 17:16:15 2006 Subject: [Histonet] Diminishing support services In-Reply-To: <01M3E288IDTM8WXJDW@Macon2.Mercer.edu> References: <44885934020000770000062A@hcnwgwds01.hh.chs> <01M3E288IDTM8WXJDW@Macon2.Mercer.edu> Message-ID: <4488693E020000770000063C@hcnwgwds01.hh.chs> Yes, some of us are using "Dragon" voice recognition, but it is not the answer to a "transcriptionist shortage". Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> Shirley Powell 06/08/06 5:26 PM >>> Some transcription can be done by voice recognition software. Although some of the medical terms may confuse the issue. A couple of my doctors use it when they are doing exams, seems to work for them. Also you may find that cross training the techs is an option too. I typed pathology reports after getting the slides out way back when life was much simpler. :) Of course you should pay them the transcriptionist salary as well. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, June 08, 2006 4:07 PM To: Hazel V Horn; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Diminishing support services Unfortunately, no. We have so little support in our accessioning/transcription area that some of us are accessioning our own consult cases and many of us are typing our own pathology reports. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Horn, Hazel V" 06/08/06 4:50 PM >>> Uh....is this a joke??? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Thursday, June 08, 2006 3:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Diminishing support services Are there any other hospitals out there where the pathologists accession cases and type their own reports? Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------------- -- The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================ == _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Thu Jun 8 21:27:02 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Thu Jun 8 21:27:11 2006 Subject: [Histonet] Neutralisation of 10% formalin using ammonia Message-ID: Following this discussion, I have some queries. I believe that Hexamethylenetetramine is a fertiliser and together with the phosphate salts used in most NBF solutions would produce a rich solution to be tipping down the sink. Would this neutralised solution result in over-load of most sewage treatment plants resulting in unwanted blue-green algae blooms? Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, 8 June 2006 2:39 AM To: Anne Van Binsbergen; Mike Kirby; Histonet (E-mail) Subject: RE: [Histonet] Neutralisation of 10% formalin using ammonia Anne: We are in the "same boat"; I also used Neutralex, but my answer was regarding as to how to make sure formalin was neutralized and even using Neutralex I tested always before discarfding it. I agree Sakura is the bast!! Hope you are well. Ren? J. Anne Van Binsbergen wrote: hey Mike and Rene! 'camel queens' use Neutralex by Sakura - its the best!!! hope you are both well - in your respective corners of the Histonet!! ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Rene J Buesa Sent: Wed 2006/06/07 06:39 PM To: Mike Kirby; Histonet (E-mail) Subject: Re: [Histonet] Neutralisation of 10% formalin using ammonia Mr. M: Schiff's reagent is perhaps the best, most sensitive and available reagent in any lab to test for the presence of aldehydes. If you "neutralized" formalin turns purplish with the addition of Schiff's reagent, it is not totally neutralized. That was my reagent of choice when I tested different formalin neutralizing products. Sakura provides a kit containing Schiff's reagent to test their neutralizing product. Ren? J. Mike Kirby wrote: Dear Histonetters. The method of neutralising 10% formalin using concentrated ammonia solution has been well documented, and no doubt used by many Labs to dispose of their formalin waste. My first question is, how does one verify that all the formalin has been neutralised? One reference states that when the pH changes from 6 to 8, then sufficient ammonia has been added, and by implication, all the formalin should have been neutralised. My second question is, would Schiffs Reagent be of any use as a double check in this methodology, because as we all know, it turns pink, even at very low concentrations of formalin, or would the final product, Hexamethylenetetramine, also react with Schiffs? Has anyone pursued this avenue? Mr.M.Kirby Johannesburg South Africa. ********************************************************************************** The views expressed in this email are, unless otherwise stated, those of the author and not those of the National Health Laboratory Services or its management. The information in this e-mail is confidential and is intended solely for the addressee. Access to this e-mail by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken or omitted in reliance on this, is prohibited and may be unlawful. Whilst all reasonable steps are taken to ensure the accuracy and integrity of information and data transmitted electronically and to preserve the confidentiality thereof, no liability or responsibility whatsoever is accepted if information or data is, for whatever reason, corrupted or does not reach its intended destination. *********************************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From cpomajzl <@t> cpllabs.com Fri Jun 9 07:39:34 2006 From: cpomajzl <@t> cpllabs.com (Chris Pomajzl) Date: Fri Jun 9 07:35:23 2006 Subject: [Histonet] muscle cross section References: <20060608202736.64493.qmail@web38214.mail.mud.yahoo.com> Message-ID: <003d01c68bc1$c6a997b0$26fca8c0@CSP> If you have darkfield capabilities, you can look at your sections under a microscope. This can also be done with brightfield, but it is easier to do with darkfield. And just to be an anal-retentive jackass, here is a list of spelling errors in your post: allot --- a lot recieve --- receive anybodies --- anybody's (can't say that I've ever seen this one, gave me a chuckle, works well for IHC) exceptable --- acceptable experiance --- experience delemma --- dilemma Just an FYI should you ever submit a resume, procedure, or report. Happy Friday. I have a case of ice-cold Tecate with lime waiting for me when I get home. Come on over if you would like to join me. GO MAVS! ----- Original Message ----- From: "Steven Coakley" To: Sent: Thursday, June 08, 2006 3:27 PM Subject: [Histonet] muscle cross section > I'm having to process and embed rodent cross section muscle. Allot of the muscle is very small and irregular pieces when I recieve it for processing. By the time I'm ready to embed it, trying to find the cross section is almost anybodies guess. Lately I've been taking shallow cuts and staining them to make sure I have a fairly exceptable cross section. Makes for allot of extra work and sometime lost tissue. Has anyone any experiance and advice for this delemma. > > Thanks, > > steve > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Fri Jun 9 07:58:25 2006 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Fri Jun 9 07:58:34 2006 Subject: [Histonet] neutrophils Message-ID: <0E394B648E5284478A6CCB78E5AFDA270171FE10@hpes1.HealthPartners.int> We are staining some research tissue (rabbitt?) for neutrophils using the chloroacetate esterase kit from Sigma with an inflamed appendix for a control. The control turned out beautiful, but, nothing is showing up in the research tissue. We did not gross in the research tissue, only processed it according to our routine processing schedule. According to the "fellow" who is doing this project, the formalin was expired on the research tissue. Does anyone have any ideas on this case?? I am at a loss and the pathologist helping the "fellow" to read these tissues out is wanting me to come up with a solution, of course!! So, HELP and thanks, as always!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From Kathy.Johnston <@t> CLS.ab.ca Fri Jun 9 08:32:17 2006 From: Kathy.Johnston <@t> CLS.ab.ca (Kathy.Johnston@CLS.ab.ca) Date: Fri Jun 9 08:32:24 2006 Subject: [Histonet] neutrophils Message-ID: <4BC300747AF87A48BCDF8E48BC2885CE01365376@mail1.calgary.com> Hi Dorothy! We routinely use this kit for our Esterases, and it works well for us. The one thing you should tell your researcher, is that this kit is not specific to just neutrophils. It is specific to any cell in the granulocytic lineage, so your basophils, and eosinphils will also stain. We have had some research material stain negatively (this was mouse in our case) so I wonder if this kit is geared to human enzymatic activity specifically. I would try calling Sigma and see it this is the case. Otherwise, the insert states that there are a few things that can cause a false negative, although if your control works, I would say that it is something to do with the tissue itself (or the fixation). I don't think that expired formalin would do it as we get samples from Cameroon on a regular basis, and the formalin in them is definitely not great, and they work fine. This may not help much, but I would try Sigma first and see if the kit has been designed for non-human tissue! Best of Luck! Kathy Johnston Tech II Special Stains Anatomic Pathology Calgary Laboratory Services #9 - 3535 Research Road NW Calgary, AB. 403-770-3572 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Webb, Dorothy L Sent: June 9, 2006 6:58 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] neutrophils We are staining some research tissue (rabbitt?) for neutrophils using the chloroacetate esterase kit from Sigma with an inflamed appendix for a control. The control turned out beautiful, but, nothing is showing up in the research tissue. We did not gross in the research tissue, only processed it according to our routine processing schedule. According to the "fellow" who is doing this project, the formalin was expired on the research tissue. Does anyone have any ideas on this case?? I am at a loss and the pathologist helping the "fellow" to read these tissues out is wanting me to come up with a solution, of course!! So, HELP and thanks, as always!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. From kewleys <@t> health.missouri.edu Fri Jun 9 10:09:13 2006 From: kewleys <@t> health.missouri.edu (Kewley, Sharyl F.) Date: Fri Jun 9 10:09:19 2006 Subject: [Histonet] RE: Histonet Digest, Vol 31, Issue 13; Warthin Starry References: Message-ID: <93D0418A67D27C47BD90CF3A0DCC39F524A250@UM-XMAIL04.um.umsystem.edu> ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of histonet-request@lists.utsouthwestern.edu Sent: Thu 6/8/2006 4:33 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 31, Issue 13 Hi! Nita, When I worked at Dr. Freeman's lab in Dallas we did the Warthin & Starry. We were very careful about glassware, we made sure that it was "acid Cleaned", we also put everything, including the pipettes, in the oven at 60 degrees overnight and took it out as we were ready to use in the stain. It worked very well, however, I have not had that kind of luck elsewhere. My favorite procedure, and I am assuming you are staining for spirochetes, is the Stiener & Steiner. I always purchase the "Modified Silver Kit" from Sigma Aldrich and the results are great. I use the same kit for the Genta procedure for helicobacter. I believe the kit's catalog number is HT101, you might want to check that number. Hope this is helpful. If you have any further questions you could call me at 573-875-9354. Godspeed, Sharyl Kewley, HT (ASCP) Columbia Regional Hospital Columbia, Missouri ---------------------------------------------------------------------- ------------------------------ Message: 5 Date: Thu, 08 Jun 2006 13:10:37 -0500 From: "Nita Searcy" Subject: [Histonet] Warthin Starry To: Message-ID: Content-Type: text/plain; charset=US-ASCII I have a tech that's having problems with the Warthin-Starry. She believes its the citric acid. Can someone share their procedure (the simpler the better) and specifics on citric acid? I believe that she is getting a large amt. of precipitate when she mixes silver?? Thanks To post to this group, send email to ihcrg@googlegroups.com >>To unsubscribe from this group, send email to >>ihcrg-unsubscribe@googlegroups.com >>For more options, visit this group at http://groups.google.com/group/ihcrg >>-~----------~----~----~----~------~----~------~--~--- ------------------------------ From pruegg <@t> ihctech.net Fri Jun 9 11:11:10 2006 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Fri Jun 9 11:11:07 2006 Subject: [Histonet] whole eyes Message-ID: <200606091610.k59GAw9U003952@chip.viawest.net> Dear Eye People, It has been about 25 years since I processed eyes. I just got some fresh whole rabbit eyes that I want to fix and process into GMA. They came in a media with antibiotic in it one wet ice. I assume that I put them whole into cold formalin to maintain the shape, then at some point I will need to extract the fluid inside without collapsing so that I can process into GMA. I am going to use graded changes of the GMA as my dehydrating agent avoiding alcohol (this will be necessary for the final product which will contain hydrogel implant which does not survive solvent processing, thus the choice of GMA embedding). I seem to remember using a needle and syringe to extract some fluid and then replace it with the GMA in stages???? Thank you so much for your advise, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From cfavara <@t> niaid.nih.gov Fri Jun 9 11:30:02 2006 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID) [E]) Date: Fri Jun 9 11:30:10 2006 Subject: [Histonet] whole eyes In-Reply-To: <200606091610.k59GAw9U003952@chip.viawest.net> Message-ID: Patsy, I have been doing a fair number of eyes lately but in paraffin. I find that for optimal fixation it is helpful to inject the eye with fixative shortly after the necropsy. c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives -----Original Message----- From: Patsy Ruegg [mailto:pruegg@ihctech.net] Sent: Friday, June 09, 2006 9:11 AM To: histonet@pathology.swmed.edu Subject: [Histonet] whole eyes Dear Eye People, It has been about 25 years since I processed eyes. I just got some fresh whole rabbit eyes that I want to fix and process into GMA. They came in a media with antibiotic in it one wet ice. I assume that I put them whole into cold formalin to maintain the shape, then at some point I will need to extract the fluid inside without collapsing so that I can process into GMA. I am going to use graded changes of the GMA as my dehydrating agent avoiding alcohol (this will be necessary for the final product which will contain hydrogel implant which does not survive solvent processing, thus the choice of GMA embedding). I seem to remember using a needle and syringe to extract some fluid and then replace it with the GMA in stages???? Thank you so much for your advise, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ebreisch <@t> chsd.org Fri Jun 9 11:33:19 2006 From: Ebreisch <@t> chsd.org (Breisch, Eric) Date: Fri Jun 9 11:33:28 2006 Subject: [Histonet] policy and procedures Message-ID: <00D7298B3825D511BF540008C75F8A3C0CAD8300@excluster.chsd.org> Does anyone happen to have a policy and procedure regarding notification of the CAP office whenever the laboratory finds itself the subject of an investigation by a state or federal agency or adverse media attention related to laboratory performance? If you do would you please share the procedure with our laboratory? Many thanks in advance. Regards, Eric A. Breisch, Ph.D. Clinical Anatomist Department of Pathology Children's Hospital and Health Center 3020 Children's Way San Diego, CA 92123 Associate Clinical Professor of Anatomy Department of Surgery UCSD School of Medicine La Jolla, CA ebreisch@chsd.org From gu.lang <@t> gmx.at Fri Jun 9 11:40:00 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Jun 9 11:40:00 2006 Subject: [Histonet] Her2 FISH with or without CEP Message-ID: <000101c68be3$59954670$eeeea8c0@SERVER01> Hi histonetters, I have a question about the sense of using centromer-probes in the Her2-test. One opinion says, that a testsystem without centromer-probe, to recognise a polysomie, is not seriously. Only the real amplifications should be treated with herceptin, because the others don't respond to it. The harsh medication could even make more harms. The other opinion says. The number of polysomies under the Her3+ cases is too little to worry about. What do you think about it? Gudrun Lang Histolab Akh Linz, Austria From jhaviland <@t> mdanderson.org Fri Jun 9 12:29:00 2006 From: jhaviland <@t> mdanderson.org (jhaviland@mdanderson.org) Date: Fri Jun 9 12:29:09 2006 Subject: [Histonet] Vectastain elite kits Message-ID: Dear Histonetters: I am hoping someone has an answer : ) I had a crazy question run through my brain. Is my thinking cap flawed today...I am using a Vectastain Elite kit that is rabbit and one that is mouse. Is the ABC reagent the same in each kit and could be used interchangeable for either kit ( then I could save on making only one) or is it animal specific? Thanks Joie From sbreeden <@t> nmda.nmsu.edu Fri Jun 9 13:10:19 2006 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Jun 9 13:10:24 2006 Subject: [Histonet] Let's FLAME Message-ID: Lets flame the guy what said we was bad spellers! Whazzup with that? I done ben flamed for less - go get em! And than, y'all go check yur rezumaes...reesumees...story of yur life...oh, hell - curriculum vitae! Happy Flaming Friday! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87108 From sae2001 <@t> med.cornell.edu Fri Jun 9 13:14:30 2006 From: sae2001 <@t> med.cornell.edu (Scott A. Ely) Date: Fri Jun 9 13:14:40 2006 Subject: [Histonet] neutrophils Message-ID: <6b86b9677465.44898246@med.cornell.edu> Dorothy, Because neutropils contain myeloperoxidase, it you don't quench the tissue with h2o2 (as is done in IHC), they will turn DAB brown. This is a pretty good and simple way of detecting neutropils. I don't have a protocol off the top of my head, but if you do what is otherwise an IHC stain, only skip the h2o2 and don't use any antibody, all the neutrophils will be brown; other cells don't generally have any type of peroxidase. Also, at least in humans, CD15 is pretty neutropil specific and a good marker---if you want to go the IHC route. Scott Ely, MD MPH Section of Hematopathology Department of Pathology Weill Medical College of Cornell University New York Presbyterian Hospital 525 E. 68th Street New York, NY 10021 PH: 212-746-2442 Legal Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the original intended recipient(s) selected by Dr. Ely and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the recipient specified by Dr. Ely, please contact the sender by reply e-mail and destroy all copies of the original message. ----- Original Message ----- From: histonet-request@lists.utsouthwestern.edu Date: Friday, June 9, 2006 1:00 pm Subject: Histonet Digest, Vol 31, Issue 14 From gradice <@t> richmond.edu Fri Jun 9 13:26:37 2006 From: gradice <@t> richmond.edu (Gary Radice) Date: Fri Jun 9 13:27:34 2006 Subject: [Histonet] fixing sponge larvae for in situs Message-ID: <464184F7-0FAF-4655-AB73-F842D6B22E76@richmond.edu> I have a colleague who wants to do in situ hybridizations on sponge larvae (yes, sponges are animals and they have larval forms!). She tried fixing some in 4% paraformaldehyde in sea water, and the larvae exploded. She also tried Carnoy's fix, and 4% PFA in Millonig's phosphate buffer, and the same thing happened. The only thing I could think of to suggest was dropping to 1 or 2% PFA in sea water. I've seen references to using Bouins for light microscopy or osmium tetroxide for TEM, but I don't think either of these approaches would be good for in situs. Any other ideas? Gary P. Radice gradice@richmond.edu Department of Biology 804-289-8107 (voice) University of Richmond 804-289-8233 (FAX) Richmond VA 23173 http://www.richmond.edu/~gradice From ahaines <@t> vims.edu Fri Jun 9 13:53:54 2006 From: ahaines <@t> vims.edu (Ashley Haines) Date: Fri Jun 9 13:54:07 2006 Subject: FW: [Histonet] fixing sponge larvae for in situs Message-ID: <008201c68bf6$0e558860$be07468b@campus.vims.edu> Here are some thoughts that I am passing on from a friend with experience in this area: -----Original Message----- From: David Gauthier [mailto:gauthier@vims.edu] Sent: Friday, June 09, 2006 2:51 PM To: 'Ashley Haines' Subject: RE: [Histonet] fixing sponge larvae for in situs It sounds like a classical osmolality problem. The fact that they blow up in seawater is a little weird, but I'd suggest checking the osmolality of the fixative vs. whatever the larvae come out of. It may be that the 4%PFA is enough to change the osmolality sufficiently and the larvae are just that fragile. For something that small, I would think that 1-2% PFA would be sufficient. You might also ask if their PFA is fresh. It can do strange things when it gets old. Bouins and osmium are both really bad ideas. Dave -----Original Message----- From: Ashley Haines [mailto:ahaines@vims.edu] Sent: Friday, June 09, 2006 2:43 PM To: 'David Gauthier' Subject: FW: [Histonet] fixing sponge larvae for in situs Any thoughts or experience here? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gary Radice Sent: Friday, June 09, 2006 2:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fixing sponge larvae for in situs I have a colleague who wants to do in situ hybridizations on sponge larvae (yes, sponges are animals and they have larval forms!). She tried fixing some in 4% paraformaldehyde in sea water, and the larvae exploded. She also tried Carnoy's fix, and 4% PFA in Millonig's phosphate buffer, and the same thing happened. The only thing I could think of to suggest was dropping to 1 or 2% PFA in sea water. I've seen references to using Bouins for light microscopy or osmium tetroxide for TEM, but I don't think either of these approaches would be good for in situs. Any other ideas? Gary P. Radice gradice@richmond.edu Department of Biology 804-289-8107 (voice) University of Richmond 804-289-8233 (FAX) Richmond VA 23173 http://www.richmond.edu/~gradice _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cpomajzl <@t> cpllabs.com Fri Jun 9 14:00:33 2006 From: cpomajzl <@t> cpllabs.com (Chris Pomajzl) Date: Fri Jun 9 13:56:24 2006 Subject: [Histonet] Let's FLAME References: Message-ID: <000901c68bf6$fce37aa0$26fca8c0@CSP> Yeah, screw that guy! What a jerk! I hate those pompuss pricks that think their better then every body. Nevermind that englisch is are primary langwedge. ----- Original Message ----- From: "Breeden, Sara" To: Sent: Friday, June 09, 2006 1:10 PM Subject: [Histonet] Let's FLAME Lets flame the guy what said we was bad spellers! Whazzup with that? I done ben flamed for less - go get em! And than, y'all go check yur rezumaes...reesumees...story of yur life...oh, hell - curriculum vitae! Happy Flaming Friday! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87108 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dearolf <@t> hendrix.edu Fri Jun 9 14:20:48 2006 From: Dearolf <@t> hendrix.edu (Dearolf, Jennifer) Date: Fri Jun 9 14:20:54 2006 Subject: [Histonet] Freezing small pieces of skeletal muscle tissue Message-ID: <04BA43F7E88D84449A6B8234210833AC040EB8BA@hnxas02.hendrix.local> Greetings, Histonetters, I realize that freezing skeletal muscle tissue has been a topic many times before, but in going through the archives, I could not find anyone that was using the method that we are (and maybe that's the problem, since we are having issues with freezing artifact). Thus, I am writing the list to see if anyone has any suggestions for how we might modify our methods to have better results. We collect our tissue fresh and mount it in 5% gum tragacanth on cork blocks (6mm thick). To freeze the tissue, we put some isopentane in a frozen juice can (with the juice removed, of course!) and place the juice can in liquid nitrogen. The liquid nitrogen is contained in a small, styrofoam cooler. When the isopentane gets slushy, we freeze our samples for 60 seconds. We then toss the frozen samples into a cryostat set at -21C. Once the samples have warmed up and any liquid isopentane has evaporated, we wrap our samples in parafilm and store in cardboard boxes in a -70C freezer. To cut, we move the boxes back into the cryostat and allow to wam up to -21C for approximately an hour. Then, we cut. I used this method successfully with larger pieces of muscle, but we are now attempting to freeze some mouse and guinea pig muscles (diaphragm, scalenus, rectus thoracis, and rectus abdominus), and we are getting a ton of freezing artifact. To try to prevent the artifact, we reduced the freezing time to 20 seconds. We also tried wrapping our samples in small pieces of liver. Neither method seems to work consistently. I am at my wits end. Everytime I think we have the freezing artifact beaten, it comes back with a vengence. I would appreciate any advice. I will keep track of the temperature of the isopentane, since this seems to be an important variable to control (according to the discussions in the archives). I would like to ask, however, should the temperature be -150 or -160C when you freeze the muscle? Thanks for your help! Sincerely, Jenn Jennifer Dearolf, Ph.D. Assistant Professor Biology Department Hendrix College 1600 Washington Avenue Conway, AR 72032 (501) 450-4530 (office) (501) 450-4547 (fax) From froyer <@t> bitstream.net Fri Jun 9 15:05:22 2006 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Jun 9 15:05:33 2006 Subject: [Histonet] Let's FLAME In-Reply-To: <000901c68bf6$fce37aa0$26fca8c0@CSP> Message-ID: <002901c68c00$0a947d30$7701a80a@Ford> OH NO!! ON NO!! It's the spiling & gramma Police!!! RUN EVERBOUDY!! RUN!! Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. Golden Valley, MN 55427-3601 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chris Pomajzl Sent: Friday, June 09, 2006 2:01 PM To: HISTONET Subject: Re: [Histonet] Let's FLAME Yeah, screw that guy! What a jerk! I hate those pompuss pricks that think their better then every body. Nevermind that englisch is are primary langwedge. ----- Original Message ----- From: "Breeden, Sara" To: Sent: Friday, June 09, 2006 1:10 PM Subject: [Histonet] Let's FLAME Lets flame the guy what said we was bad spellers! Whazzup with that? I done ben flamed for less - go get em! And than, y'all go check yur rezumaes...reesumees...story of yur life...oh, hell - curriculum vitae! Happy Flaming Friday! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87108 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Jun 9 16:08:53 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 9 16:08:58 2006 Subject: [Histonet] fixing sponge larvae for in situs In-Reply-To: <464184F7-0FAF-4655-AB73-F842D6B22E76@richmond.edu> Message-ID: <20060609210853.36154.qmail@web61219.mail.yahoo.com> Gary: Larvae of Coelenterata and Platyhelmintes have to be narcotized before fixation, or they will explode. This is what is probably happening to the sponges planulae larvae. They could be narcotized either with menthol or a 2% hydrate chloral solution. Afterwards they could be fixed with a hot mercuric-acetic solution. Perhaps NBF could be used also. So, the kid of the thing is to narcotize the larvae before fixing them. Hope this will help you. Ren? J. Gary Radice wrote: I have a colleague who wants to do in situ hybridizations on sponge larvae (yes, sponges are animals and they have larval forms!). She tried fixing some in 4% paraformaldehyde in sea water, and the larvae exploded. She also tried Carnoy's fix, and 4% PFA in Millonig's phosphate buffer, and the same thing happened. The only thing I could think of to suggest was dropping to 1 or 2% PFA in sea water. I've seen references to using Bouins for light microscopy or osmium tetroxide for TEM, but I don't think either of these approaches would be good for in situs. Any other ideas? Gary P. Radice gradice@richmond.edu Department of Biology 804-289-8107 (voice) University of Richmond 804-289-8233 (FAX) Richmond VA 23173 http://www.richmond.edu/~gradice _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From LuckG <@t> empirehealth.org Fri Jun 9 16:43:59 2006 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Fri Jun 9 16:44:05 2006 Subject: [Histonet] Old Microtome Message-ID: <6BB8BC4519AAB844B174FC739A679BBC1C3069@IRMEXCH01.irm.inhs.org> Hello All, A group of physicians and technicians are going on a mission trip to a country in Africa in September. They approached me about acquiring an old unused microtome for them to take with them as there is no access to histology services where they are going. They have all the other equipment they need for histology services. If anyone has a microtome (functional not fancy) they would be willing to donate to this worthwhile mission I would be more than happy to pay for and facilitate the shipping of the microtome to them. Thanks, Greg Greg Luck Anatomic Path Spvr Deaconess Med Cntr 800 W. 5th Ave Spokane, WA 99204 Offc 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmc.org From jnocito <@t> satx.rr.com Fri Jun 9 06:00:44 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Jun 9 18:10:02 2006 Subject: [Histonet] DAB disposal Message-ID: <000501c68c19$d21e25e0$0b69ce44@yourxhtr8hvc4p> with the risk of sounding stupid (which happens to me often) how are people disposing their DAB contaminated Ventana waste? I mean, there is so little actual DAB present. I refuse to answer any questions that might incriminate me. I know what you are thinking. I have a new safety office who just happens to be certified in hazmat (yes, for those who know me, it's Hector). Hector keeps bugging me about it. For those who don't know us, Hector and I are joined at the hip. Last year in Ft. Lauderdale, everyone knew something was wrong because they saw Hector without me. No, we are not homosexuals, not that it matters. Hector was my histology instructor and we are really, really close friends. Oh d _ _ m, I'm sure I just offended somebody. I'm leaving now before I say something else wrong. It must be Friday. Why do I do this to myself? Have a safe weekend y'all. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX From jnocito <@t> satx.rr.com Fri Jun 9 18:13:10 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Jun 9 18:13:16 2006 Subject: [Histonet] Let's FLAME References: <002901c68c00$0a947d30$7701a80a@Ford> Message-ID: <001401c68c1a$46a059b0$0b69ce44@yourxhtr8hvc4p> let me attam, let me attam. I'll tearem lim from lim ----- Original Message ----- From: "Ford Royer" To: "'HISTONET'" Sent: Friday, June 09, 2006 3:05 PM Subject: RE: [Histonet] Let's FLAME > OH NO!! ON NO!! > > It's the spiling & gramma Police!!! > > RUN EVERBOUDY!! RUN!! > > > > Ford M. Royer, MT(ASCP) > Minnesota Medical, Inc. > Golden Valley, MN 55427-3601 > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chris > Pomajzl > Sent: Friday, June 09, 2006 2:01 PM > To: HISTONET > Subject: Re: [Histonet] Let's FLAME > > Yeah, screw that guy! What a jerk! I hate those pompuss pricks that think > their better then every body. Nevermind that englisch is are primary > langwedge. > > ----- Original Message ----- > From: "Breeden, Sara" > To: > Sent: Friday, June 09, 2006 1:10 PM > Subject: [Histonet] Let's FLAME > > > Lets flame the guy what said we was bad spellers! Whazzup with that? I > done ben flamed for less - go get em! And than, y'all go check yur > rezumaes...reesumees...story of yur life...oh, hell - curriculum vitae! > > > > Happy Flaming Friday! > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 700 > > Albuquerque, NM 87108 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.394 / Virus Database: 268.8.3/359 - Release Date: 6/8/2006 > > From zwiegers <@t> interchange.ubc.ca Fri Jun 9 19:21:39 2006 From: zwiegers <@t> interchange.ubc.ca (zwiegers) Date: Fri Jun 9 19:21:44 2006 Subject: [Histonet] Cryoprotection Message-ID: <1447968.1149898899292.JavaMail.myubc2@brahms.my.ubc.ca> Good day all, Recently, we have sacrificed and perfused a batch of mice, and due to inattentiveness, some tissues (brain, spinal cord, liver, and kidney) were protected in 3% sucrose (instead of 30%). These were frozen and stored at -80 C for over a week. As soon as we started slicing the brain(s), we noticed that the tissue was shattering. At first we tried to reduce the temperature by keeping the tissue at - 30 C overnight. The tissue was still shattering when we tried to slice it. Now, since we used expired OCT, we assumed that this could have caused the problem. We then tried to use new OCT on a batch of mice that were not frozen yet (using 3% sucrose)and the results were the same. Fortunately, we have not lost all of our tissue and they will just be transferred to a 30% sucrose solution. Now, the question is, what can we do with the frozen tissue? Is it at all possible to thaw the OCT and transfer the organs to a 30% sucrose soltution with minimal damage to tissue integrity? Thank you so much in advance Sara Sarband Pierre Zwiegers From omnivore98 <@t> yahoo.com Fri Jun 9 19:27:02 2006 From: omnivore98 <@t> yahoo.com (Heather Renko) Date: Fri Jun 9 19:27:07 2006 Subject: [Histonet] Richards Query-Diminishing support services Message-ID: <20060610002702.92251.qmail@web31314.mail.mud.yahoo.com> Richard, I can say that I have seen pathologists accession in their own cases at several different institutions in the late evening and on the weekends due to lack of staffing. I think its a shame having such highly trained pathologists doing tasks that a lab assistant or histology technician should be doing. Most histology techs are overworked too, so who is left? Unfortunately, in today's lab its not always easy to budget a FTE for these tasks throughout the day and weekends. I know in many instances this is why we see so much turnover with histology techs is that many times we end up being the lab assistant amongst the many other hats we wear. The pathologists need to be more assertive with the hospital administrators and insist on more FTE's. In a perfect world, I sure would hope to have more ancillary staff to help free up my pathologists. Heather __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From int09018 <@t> alphahunt.com Fri Jun 9 22:09:36 2006 From: int09018 <@t> alphahunt.com (HCS) Date: Fri Jun 9 22:10:02 2006 Subject: [Histonet] re:help needed for older tissue processor Message-ID: <000601c68c3b$4e78da60$6401a8c0@hp> Hi, I am looking for leads or any information on the RMC MVP 1 tissue processor also know as the Instrumentation Laboratory MVP. It is Model 1505. My biggest problem is that I need the code that allows me to go into the calibration and service screen on the menu. If anyone knows where I might try or who might have a similar machine I would appreciate knowing how to get in touch with them. The machine was originally sold by Fisher Scientific and was taken over by Instrumentation Laboratory and later it went to Ventana or RMC I am not sure which. I am in the Seattle area. So far with internet searches etc. I have not come up with much information. I am just hoping someone out there in histoland can come to my rescue. Thanks LeRoy Brown HT (ASCP) HTL 360-966-7300 www.histocs.com From yeepengtiang <@t> hotmail.com Fri Jun 9 23:53:18 2006 From: yeepengtiang <@t> hotmail.com (tiang yeepeng) Date: Fri Jun 9 23:53:24 2006 Subject: [Histonet] Immunofluorescence in exfoliated cells from the cervix uterine Message-ID: Dear all, I am currently staining for the E6 protein in exfoliated cells obtained from the uterine cervix by indirect immunofluorescence method. However, the noise was found to be terrible due to the presence of dirts and other particles eg: bacterias commonly found in the cervix and also the actinomycetes. Repeated washings with PBS were not able to rid those particles. Can anyone please advise? Thanks! With regards, TIANG YEE PENG Department of Medical Microbiology, Faculty of Medicine, University of Malaya. _________________________________________________________________ Express yourself instantly with MSN Messenger! Download today it's FREE! http://messenger.msn.click-url.com/go/onm00200471ave/direct/01/ From RCHIOVETTI <@t> aol.com Sat Jun 10 08:41:58 2006 From: RCHIOVETTI <@t> aol.com (RCHIOVETTI@aol.com) Date: Sat Jun 10 08:42:12 2006 Subject: [Histonet] re:help needed for older tissue processor Message-ID: <4bf.f90ce9.31bc2626@aol.com> In a message dated 6/9/2006 8:10:45 PM US Mountain Standard Time, int09018@alphahunt.com writes: > Hi, I am looking for leads or any information on the RMC MVP 1 tissue > processor also know as the Instrumentation Laboratory MVP. It is Model 1505. My > biggest problem is that I need the code that allows me to go into the > calibration and service screen on the menu. LeRoy, Call Ventana Medical Systems, (800) 227-2155 and ask for Tom Kennedy. He should be able to help you with the service code. Good Luck! Bob Robert (Bob) Chiovetti, Ph.D. Owner The Microscope Works Arizona's Microscopy Resource 132 North Elster Drive Tucson, AZ 85710-3212 USA Tel./Fax 520-546-4986 Manufacturers' Representative Member, Arizona Small Business Association - ASBA From LuckG <@t> empirehealth.org Sat Jun 10 16:17:05 2006 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Sat Jun 10 16:17:13 2006 Subject: [Histonet] re:help MVP/Ventana tissue processors Message-ID: <6BB8BC4519AAB844B174FC739A679BBC1C306C@IRMEXCH01.irm.inhs.org> To Leroy et.al., Try the code numbers 762 (and press enter on the keypad). I have both an older RMC instrument as well as a newer Ventana Renaissance. I can access the service diagnostics of both instruments with this code. I use this feature frequently to check that my remote alarms are functioning. The way I easily remember this code sequence is that 762 on your telephone keypad is (you guessed it) "RMC". Contact me if you need any help with your processor as both I and one of our biomed techs have a lot of experience with these instruments. Good luck, Greg Greg Luck, BS, HT(ASCP) Anatomic Pathology Supervisor Deaconess Medical Center 800 W. 5th Ave Spokane, WA 99204 Phone 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmc.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of RCHIOVETTI@aol.com Sent: Saturday, June 10, 2006 6:42 AM To: int09018@alphahunt.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] re:help needed for older tissue processor In a message dated 6/9/2006 8:10:45 PM US Mountain Standard Time, int09018@alphahunt.com writes: > Hi, I am looking for leads or any information on the RMC MVP 1 tissue > processor also know as the Instrumentation Laboratory MVP. It is > Model 1505. My biggest problem is that I need the code that allows me > to go into the calibration and service screen on the menu. LeRoy, Call Ventana Medical Systems, (800) 227-2155 and ask for Tom Kennedy. He should be able to help you with the service code. Good Luck! Bob Robert (Bob) Chiovetti, Ph.D. Owner The Microscope Works Arizona's Microscopy Resource 132 North Elster Drive Tucson, AZ 85710-3212 USA Tel./Fax 520-546-4986 Manufacturers' Representative Member, Arizona Small Business Association - ASBA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From katri <@t> cogeco.ca Sun Jun 11 18:29:07 2006 From: katri <@t> cogeco.ca (Katri Tuomala) Date: Sun Jun 11 18:29:12 2006 Subject: [Histonet] Freezing small pieces of skeletal muscle tissue References: <04BA43F7E88D84449A6B8234210833AC040EB8BA@hnxas02.hendrix.local> Message-ID: <006701c68dae$d5e7ec10$6a9a9618@Katri> Jenn, I'm sure you'll get some good advice after the weekend from people who do this all the time. My initial thought is, that your specimen should go from isopentane directly to -70C, not -20C, unless you cut it then. When you transfer the tissue to -70C, ice ctystal artifact will form, when tissue freezes slowly from -20 to -70. Just a thought... Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "Dearolf, Jennifer" To: Sent: Friday, June 09, 2006 3:20 PM Subject: [Histonet] Freezing small pieces of skeletal muscle tissue Greetings, Histonetters, I realize that freezing skeletal muscle tissue has been a topic many times before, but in going through the archives, I could not find anyone that was using the method that we are (and maybe that's the problem, since we are having issues with freezing artifact). Thus, I am writing the list to see if anyone has any suggestions for how we might modify our methods to have better results. We collect our tissue fresh and mount it in 5% gum tragacanth on cork blocks (6mm thick). To freeze the tissue, we put some isopentane in a frozen juice can (with the juice removed, of course!) and place the juice can in liquid nitrogen. The liquid nitrogen is contained in a small, styrofoam cooler. When the isopentane gets slushy, we freeze our samples for 60 seconds. We then toss the frozen samples into a cryostat set at -21C. Once the samples have warmed up and any liquid isopentane has evaporated, we wrap our samples in parafilm and store in cardboard boxes in a -70C freezer. To cut, we move the boxes back into the cryostat and allow to wam up to -21C for approximately an hour. Then, we cut. I used this method successfully with larger pieces of muscle, but we are now attempting to freeze some mouse and guinea pig muscles (diaphragm, scalenus, rectus thoracis, and rectus abdominus), and we are getting a ton of freezing artifact. To try to prevent the artifact, we reduced the freezing time to 20 seconds. We also tried wrapping our samples in small pieces of liver. Neither method seems to work consistently. I am at my wits end. Everytime I think we have the freezing artifact beaten, it comes back with a vengence. I would appreciate any advice. I will keep track of the temperature of the isopentane, since this seems to be an important variable to control (according to the discussions in the archives). I would like to ask, however, should the temperature be -150 or -160C when you freeze the muscle? Thanks for your help! Sincerely, Jenn Jennifer Dearolf, Ph.D. Assistant Professor Biology Department Hendrix College 1600 Washington Avenue Conway, AR 72032 (501) 450-4530 (office) (501) 450-4547 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TillRenee <@t> uams.edu Mon Jun 12 06:56:50 2006 From: TillRenee <@t> uams.edu (Till, Renee) Date: Mon Jun 12 06:56:47 2006 Subject: [Histonet] RE:Vectastain Elite kits Message-ID: <11F927674DEBDC43B960809A7403C5D2012AC5F6@MAILPED.ad.uams.edu> It's always worked fine for me. If I remember correctly, it does say something in the package insert about not exchanging reagents from different kits because they are optimized for that kit, but I've never had any problems. I've even used the blocking serum and the secondary from two different kits of the same species. Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72002 Office (501)364-2785 Fax (501)364-3161 Message: 1 Date: Fri, 9 Jun 2006 12:29:00 -0500 From: jhaviland@mdanderson.org Subject: [Histonet] Vectastain elite kits To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Dear Histonetters: I am hoping someone has an answer : ) I had a crazy question run through my brain. Is my thinking cap flawed today...I am using a Vectastain Elite kit that is rabbit and one that is mouse. Is the ABC reagent the same in each kit and could be used interchangeable for either kit ( then I could save on making only one) or is it animal specific? Thanks Joie ================================================================================================ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ================================================================================================ From TMcNemar <@t> lmhealth.org Mon Jun 12 07:58:47 2006 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Mon Jun 12 07:59:08 2006 Subject: [Histonet] DAB disposal Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F3C9@lmhsmail.lmhealth.org> I have the Dako unit. It separates the hazardous/non-hazardous waste. The DAB goes into a 20L carboy that is then hauled off-site for disposal. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Joe Nocito Sent: Friday, June 09, 2006 7:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DAB disposal with the risk of sounding stupid (which happens to me often) how are people disposing their DAB contaminated Ventana waste? I mean, there is so little actual DAB present. I refuse to answer any questions that might incriminate me. I know what you are thinking. I have a new safety office who just happens to be certified in hazmat (yes, for those who know me, it's Hector). Hector keeps bugging me about it. For those who don't know us, Hector and I are joined at the hip. Last year in Ft. Lauderdale, everyone knew something was wrong because they saw Hector without me. No, we are not homosexuals, not that it matters. Hector was my histology instructor and we are really, really close friends. Oh d _ _ m, I'm sure I just offended somebody. I'm leaving now before I say something else wrong. It must be Friday. Why do I do this to myself? Have a safe weekend y'all. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lcastillos <@t> icoria.com Mon Jun 12 08:12:17 2006 From: lcastillos <@t> icoria.com (Castillos, Luminita) Date: Mon Jun 12 08:12:41 2006 Subject: [Histonet] Tissue control for CD56 Message-ID: Good morning everyone, I am working with human endometrial FFPE tissue and I am doing IHC for NK using CD56 monoclonal antibody. I would like to ask which will be a good positive tissue control and a negative tissue control for CD56?. Also, if there is a vendor from where I can buy these tissue slides?. Thank you so much. Sincerely, Luminita Luminita Castillos, Ph.D. Research Scientist Cogenics(r), A Division of Clinical Data(r) Comprehensive Pharmacogenomics & Molecular Services(tm) 108 Alexader Dr. RTP, NC 27709 Tel: 919-425-2967 www.cogenics.com From vanann702 <@t> skmc.gov.ae Mon Jun 12 08:24:03 2006 From: vanann702 <@t> skmc.gov.ae (Anne Van Binsbergen) Date: Mon Jun 12 08:23:30 2006 Subject: [Histonet] DAB disposal Message-ID: Hi Tom Today we became proud owners of a brand new Dako unit and I had that exact thought in mind - what to do with a 20 litre bottle of dab-contaminated waste....every couple of weeks - here in the UAE we have little choice as there are no federal laws and no off-site waste companies to cart waste off even for a fee. I am stuck until someone in authority writes the book of rules. I just know that one of you will go 'gasp!shock!horror'! and insist that I be pro-active - I am - some of you know me to be 'tenacious' - this is correct - but even that has not helped me here. Advice has been offered, best practice quoted, OH&S quoted, internet sites accessed, printed, handed over in report form, promises are made by those in authority and then broken. We stockpile safely off site and wait This is true for ALL toxic waste in this laboratory, as well as the rest of the labs, together with mercury filled blood pressure cuffs, some broken, 'expired' rat poison, old mercury thermometers, 'unknown' unlabelled chemicals from shut down labs...some scary stuff But - I digress - back to the DAB....and how to manage the growing volume of dab waste..... Any suggestions.....??? Anyone.....??? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Monday, June 12, 2006 4:59 PM To: Joe Nocito; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] DAB disposal I have the Dako unit. It separates the hazardous/non-hazardous waste. The DAB goes into a 20L carboy that is then hauled off-site for disposal. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Joe Nocito Sent: Friday, June 09, 2006 7:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DAB disposal with the risk of sounding stupid (which happens to me often) how are people disposing their DAB contaminated Ventana waste? I mean, there is so little actual DAB present. I refuse to answer any questions that might incriminate me. I know what you are thinking. I have a new safety office who just happens to be certified in hazmat (yes, for those who know me, it's Hector). Hector keeps bugging me about it. For those who don't know us, Hector and I are joined at the hip. Last year in Ft. Lauderdale, everyone knew something was wrong because they saw Hector without me. No, we are not homosexuals, not that it matters. Hector was my histology instructor and we are really, really close friends. Oh d _ _ m, I'm sure I just offended somebody. I'm leaving now before I say something else wrong. It must be Friday. Why do I do this to myself? Have a safe weekend y'all. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> pathreflab.com Mon Jun 12 08:57:32 2006 From: jnocito <@t> pathreflab.com (Joe Nocito) Date: Mon Jun 12 08:55:32 2006 Subject: [Histonet] CAP question on humidity levels Message-ID: Okay, I can't stay quiet any longer. The new general checklist question asks if the temperature and humidity levels are monitored. Okay, but they don't state a range or what to do if the temp & humidity are out of range. Has any one come up with an idea? And people wonder why I have a problem with CAP. Happy Monday. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX From rjbuesa <@t> yahoo.com Mon Jun 12 09:15:33 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jun 12 09:15:40 2006 Subject: [Histonet] Tissue control for CD56 In-Reply-To: Message-ID: <20060612141533.17828.qmail@web61216.mail.yahoo.com> Luminita: For CD56 I used "brain" or "central nervous system" in general as a positive control.I never used any specific "negative" tissue, I just ran the negative slide without the primary Ab. The CD56 I used was from Novocastra with HIER at pH6 Hope this will help you. Ren? J. "Castillos, Luminita" wrote: Good morning everyone, I am working with human endometrial FFPE tissue and I am doing IHC for NK using CD56 monoclonal antibody. I would like to ask which will be a good positive tissue control and a negative tissue control for CD56?. Also, if there is a vendor from where I can buy these tissue slides?. Thank you so much. Sincerely, Luminita Luminita Castillos, Ph.D. Research Scientist Cogenics(r), A Division of Clinical Data(r) Comprehensive Pharmacogenomics & Molecular Services(tm) 108 Alexader Dr. RTP, NC 27709 Tel: 919-425-2967 www.cogenics.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From abright <@t> brightinstruments.com Mon Jun 12 09:28:27 2006 From: abright <@t> brightinstruments.com (Alan Bright) Date: Mon Jun 12 09:17:32 2006 Subject: [Histonet] Cryoprotection Message-ID: Dear Pierre, Brain should be sectioned at -8 to -12?C and if possible have the knife & anti-roll plate around -20?C. You are way too cold @ -30?C Liver & Kidney should be no lower than -18?C lower and you will just get dust. I hope this helps if any more info is required please get back to me. Happy sectioning III Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype User ID: dazzle0 -----Original Message----- From: zwiegers [mailto:zwiegers@interchange.ubc.ca] Sent: 10 June 2006 01:22 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryoprotection Good day all, Recently, we have sacrificed and perfused a batch of mice, and due to inattentiveness, some tissues (brain, spinal cord, liver, and kidney) were protected in 3% sucrose (instead of 30%). These were frozen and stored at -80 C for over a week. As soon as we started slicing the brain(s), we noticed that the tissue was shattering. At first we tried to reduce the temperature by keeping the tissue at - 30 C overnight. The tissue was still shattering when we tried to slice it. Now, since we used expired OCT, we assumed that this could have caused the problem. We then tried to use new OCT on a batch of mice that were not frozen yet (using 3% sucrose)and the results were the same. Fortunately, we have not lost all of our tissue and they will just be transferred to a 30% sucrose solution. Now, the question is, what can we do with the frozen tissue? Is it at all possible to thaw the OCT and transfer the organs to a 30% sucrose soltution with minimal damage to tissue integrity? Thank you so much in advance Sara Sarband Pierre Zwiegers _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Mon Jun 12 10:23:53 2006 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Mon Jun 12 10:24:00 2006 Subject: [Histonet] CAP question on humidity levels Message-ID: Joe, I posted a while ago on this same topic. It's just another regulation that some CAP shut-in came up with while hanging out in a dark room trying to justify his/her job. CAP just can't handle not coming up with at least a couple more crazy things to tack onto that CAP checklist every year. Next year I hear we'll need to note barometric pressure, solar wind levels & sunspot activity. Since they don't offer a range, I would suggest just writing your readings down in a tablet so that you can say you have them. Have Fun, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Joe Nocito Sent: Monday, June 12, 2006 7:58 AM To: Histonetters Subject: [Histonet] CAP question on humidity levels Okay, I can't stay quiet any longer. The new general checklist question asks if the temperature and humidity levels are monitored. Okay, but they don't state a range or what to do if the temp & humidity are out of range. Has any one come up with an idea? And people wonder why I have a problem with CAP. Happy Monday. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Mon Jun 12 10:28:57 2006 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Mon Jun 12 10:29:11 2006 Subject: [Histonet] DAB disposal Message-ID: <0BE6ADFAE4E7E04496BF21ABD346628005653BC4@EXCHANGE1.huntingtonhospital.com> We have our licensed hazardous waste hauler take it. I know there's not much DAB in the waste, but there's also the oily liquid coverslip that I don't want to pour down the drain. Laurie Colbert -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Friday, June 09, 2006 4:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DAB disposal with the risk of sounding stupid (which happens to me often) how are people disposing their DAB contaminated Ventana waste? I mean, there is so little actual DAB present. I refuse to answer any questions that might incriminate me. I know what you are thinking. I have a new safety office who just happens to be certified in hazmat (yes, for those who know me, it's Hector). Hector keeps bugging me about it. For those who don't know us, Hector and I are joined at the hip. Last year in Ft. Lauderdale, everyone knew something was wrong because they saw Hector without me. No, we are not homosexuals, not that it matters. Hector was my histology instructor and we are really, really close friends. Oh d _ _ m, I'm sure I just offended somebody. I'm leaving now before I say something else wrong. It must be Friday. Why do I do this to myself? Have a safe weekend y'all. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From fawn <@t> cs.cmu.edu Mon Jun 12 10:32:57 2006 From: fawn <@t> cs.cmu.edu (Fawn Jones) Date: Mon Jun 12 10:33:05 2006 Subject: [Histonet] viability question Message-ID: <448D8929.3000809@cs.cmu.edu> Dear histonetters, I am looking for some information for a friend. She wants to find out if her tissues (aortas) are live or not. Does anyone have any experience performing viability stains on tissue samples? She tried the Molecular Probes Live/Dead kit but found it insufficient due to permeabilty issues. She also suggested MTT, but we're not sure how well that stain works with tissues. Any help would be greatly appreciated. Thank you Fawn Jones HT(ASCP) From algranth <@t> u.arizona.edu Mon Jun 12 10:40:00 2006 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Mon Jun 12 10:40:09 2006 Subject: [Histonet] CAP question on humidity levels In-Reply-To: Message-ID: <4.3.2.7.2.20060612083605.00ce3e98@algranth.inbox.email.arizona.edu> I remember many years back that the lab where I worked in TX had to get a barometer in the lab as one of the outcomes of a CAP inspection and check the humidity - this wasn't in Histology but in the Chemistry lab. Maybe that section of the CAP checklist has the answer to your question. Or - maybe you could ask someone in chemistry and save yourself the work of trying to find the answer and only look it up if you have to. Andi At 10:23 AM 6/12/2006 -0500, Dawson, Glen wrote: >Joe, > >I posted a while ago on this same topic. It's just another regulation >that some CAP shut-in came up with while hanging out in a dark room trying >to justify his/her job. CAP just can't handle not coming up with at least >a couple more crazy things to tack onto that CAP checklist every >year. Next year I hear we'll need to note barometric pressure, solar wind >levels & sunspot activity. > >Since they don't offer a range, I would suggest just writing your readings >down in a tablet so that you can say you have them. > >Have Fun, > >Glen Dawson BS, HT & QIHC (ASCP) >IHC Manager >Milwaukee, WI > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Joe >Nocito >Sent: Monday, June 12, 2006 7:58 AM >To: Histonetters >Subject: [Histonet] CAP question on humidity levels > > > Okay, I can't stay quiet any longer. The new general checklist >question asks if the temperature and humidity levels are monitored. Okay, >but they don't state a range or what to do if the temp & humidity are out of >range. Has any one come up with an idea? And people wonder why I have a >problem with CAP. > >Happy Monday. > > > >Joe Nocito, BS, HT(ASCP) QIHC > >Histology Manager > >Pathology Reference Lab > >San Antonio, TX > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From JMacDonald <@t> mtsac.edu Mon Jun 12 10:58:56 2006 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Mon Jun 12 10:59:05 2006 Subject: [Histonet] CAP question on humidity levels In-Reply-To: <20060612140429.4D45F1670B0@barracuda.mtsac.edu> Message-ID: Blood bank room temperatures are critical for correct testing. This is on the general checklist, so it applies to the lab as a whole. "Joe Nocito" Sent by: histonet-bounces@lists.utsouthwestern.edu 06/12/2006 06:57 AM To "Histonetters" cc Subject [Histonet] CAP question on humidity levels Okay, I can't stay quiet any longer. The new general checklist question asks if the temperature and humidity levels are monitored. Okay, but they don't state a range or what to do if the temp & humidity are out of range. Has any one come up with an idea? And people wonder why I have a problem with CAP. Happy Monday. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From timothy.macatee <@t> med.nyu.edu Mon Jun 12 10:50:06 2006 From: timothy.macatee <@t> med.nyu.edu (Timothy Macatee) Date: Mon Jun 12 11:21:23 2006 Subject: [Histonet] Minimizing shrinkage Message-ID: I have someone who wants to measure cardiac vessel diameter. Does anyone have an idea of the best process to avoid shrinkage of the tissue? Notice, how I made no reference to any situation comedy television shows. Tim -- Tim Macatee Research Histology Core New York University School of Medicine 550 First Ave. Department of Pathology. Medical Science Building - Room 521 New York, N.Y. 10016 (212) 263-3888 From David.Edmondson <@t> christie-tr.nwest.nhs.uk Mon Jun 12 11:23:39 2006 From: David.Edmondson <@t> christie-tr.nwest.nhs.uk (Edmondson David (RBV) NHS Christie Tr) Date: Mon Jun 12 11:23:50 2006 Subject: [Histonet] Old Microtome.........knives Message-ID: <3C83687E8F6AE04792E361ABE2D385B8418129@cht-mail2-2k.xchristie.nhs.uk> In the context of limited availability of servicing, I wonder at Cambridge rockers, they were not so flash but the worst servicing was a new piece of twine and a spring. The only rocker I know of, left over, came from a cryostat set up and has a limited cutting stroke. But..... If anyone wants a collection of Heiffer knives and various grades of Alumina then please to ask. They have not rusted away in spite of twenty years and more in sheds. Also have a couple of regular stones and a rather long one. What is the smooth creamy coloured sharpening stone called, is it from somewhere like Kansas? And "Paul springs", that control the cam that engages in the ratchet advance. Dave Manchester UK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Luck, Greg D. Sent: 09 June 2006 22:44 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Old Microtome Hello All, A group of physicians and technicians are going on a mission trip to a country in Africa in September. They approached me about acquiring an old unused microtome for them to take with them as there is no access to histology services where they are going. They have all the other equipment they need for histology services. If anyone has a microtome (functional not fancy) they would be willing to donate to this worthwhile mission I would be more than happy to pay for and facilitate the shipping of the microtome to them. Thanks, Greg Greg Luck Anatomic Path Spvr Deaconess Med Cntr 800 W. 5th Ave Spokane, WA 99204 Offc 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************** This e-mail and any files transmitted with it are confidential and solely for the use of the intended recipient. If you have received this e-mail in error you should not disseminate, distribute or copy it. Please notify the sender immediately and delete this e-mail from your system. ******************************************************************************************************************* From cpomajzl <@t> cpllabs.com Mon Jun 12 11:38:38 2006 From: cpomajzl <@t> cpllabs.com (Chris Pomajzl) Date: Mon Jun 12 11:34:32 2006 Subject: [Histonet] Minimizing shrinkage References: Message-ID: <008801c68e3e$a8394580$26fca8c0@CSP> Don't put them in the pool. (sorry, had to say it) We used to infuse vessels with a plastic polymer. Perhaps if you do this, you could measure the diameter of the plastic assuming it would not shrink. Also, what type of animal are we talking about? ----- Original Message ----- From: "Timothy Macatee" To: Sent: Monday, June 12, 2006 10:50 AM Subject: [Histonet] Minimizing shrinkage > I have someone who wants to measure cardiac vessel diameter. Does anyone > have an idea of the best process to avoid shrinkage of the tissue? Notice, > how I made no reference to any situation comedy television shows. > > Tim > -- > Tim Macatee > Research Histology Core > New York University School of Medicine > 550 First Ave. > Department of Pathology. > Medical Science Building - Room 521 > New York, N.Y. 10016 > (212) 263-3888 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dsantana <@t> pmaonline.com Mon Jun 12 11:40:25 2006 From: dsantana <@t> pmaonline.com (Santana, Diane) Date: Mon Jun 12 11:39:24 2006 Subject: [Histonet] why Message-ID: <4C96AA62BADFD81180CB0002A5AD67FB07113A3E@MAILPMA> Hi I am looking for some ideas to help with a problem I am still having with processing my GI bx's. What would cause my tissue to be raw? Not all, not some, only 1 or 2 pieces. Example if I have a case with 4 bx's, maybe only 1 is raw, the other 3 are beautiful. All my solutions test at the right % and temperatures are fine. Question again, why does 1 piece of tissue come out raw, when the other pieces are fine? thanks in advance, Diane Santana PMA Haverhill, Mass. From Jackie.O'Connor <@t> abbott.com Mon Jun 12 11:50:24 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Mon Jun 12 11:50:53 2006 Subject: [Histonet] why In-Reply-To: <4C96AA62BADFD81180CB0002A5AD67FB07113A3E@MAILPMA> Message-ID: By raw I assume you mean "underprocesssed" (which isn't even a word) Anyway - do you use biopsy pads for processing? Jackie O' "Santana, Diane" Sent by: histonet-bounces@lists.utsouthwestern.edu 06/12/2006 11:40 AM To "'histonet@lists.utsouthwestern.edu'" cc Subject [Histonet] why Hi I am looking for some ideas to help with a problem I am still having with processing my GI bx's. What would cause my tissue to be raw? Not all, not some, only 1 or 2 pieces. Example if I have a case with 4 bx's, maybe only 1 is raw, the other 3 are beautiful. All my solutions test at the right % and temperatures are fine. Question again, why does 1 piece of tissue come out raw, when the other pieces are fine? thanks in advance, Diane Santana PMA Haverhill, Mass. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cwscouten <@t> myneurolab.com Mon Jun 12 11:56:24 2006 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Mon Jun 12 11:56:59 2006 Subject: [Histonet] Minimizing shrinkage Message-ID: <5784D843593D874C93E9BADCB87342AB013070CD@tpiserver03.Coretech-holdings.com> See the article in Microscopy Today. Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chris Pomajzl Sent: Monday, June 12, 2006 11:39 AM To: HISTONET Subject: Re: [Histonet] Minimizing shrinkage Don't put them in the pool. (sorry, had to say it) We used to infuse vessels with a plastic polymer. Perhaps if you do this, you could measure the diameter of the plastic assuming it would not shrink. Also, what type of animal are we talking about? ----- Original Message ----- From: "Timothy Macatee" To: Sent: Monday, June 12, 2006 10:50 AM Subject: [Histonet] Minimizing shrinkage > I have someone who wants to measure cardiac vessel diameter. Does anyone > have an idea of the best process to avoid shrinkage of the tissue? Notice, > how I made no reference to any situation comedy television shows. > > Tim > -- > Tim Macatee > Research Histology Core > New York University School of Medicine > 550 First Ave. > Department of Pathology. > Medical Science Building - Room 521 > New York, N.Y. 10016 > (212) 263-3888 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> Stowers-Institute.org Mon Jun 12 12:11:30 2006 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Mon Jun 12 12:11:51 2006 Subject: [Histonet] Re: Why (1 GI biopsy not processed, others are beautiful) Message-ID: Diane, Do you use screen cassettes? If so, do you make sure they are submerged, or do you have them loose in your tissue processor chamber? I don't know if anybody else has noticed this or has become alarmed by it, but the screen cassettes trap solution and usually have at least one trapped air bubble in there. It's theoretically possible one of your biopsies are sitting up in the air bubble and not getting solution. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From EJones <@t> Ventanamed.com Mon Jun 12 12:19:26 2006 From: EJones <@t> Ventanamed.com (Emma JONES) Date: Mon Jun 12 12:19:36 2006 Subject: [Histonet] RE: Disposal of Ventana DAB waste Message-ID: Hi Joe,=20 A specific answer to your question, regarding Ventana not DAKO waste. = The volume of DAB in the waste is very small and diluted considerably = by the other solvent free solutions. It can be thrown down the sink, washed down with plenty of water. However there is a technique for deactivation of DAB which is applicable = to any form of DAB waste. Prepare a 2 mol/litre H2SO4 solution, and a 0.2 mol/litre KMnO4 solution - Add 50 ml of each solution to the Ventana DAB waste to be destroyed - Let the 3 solutions react overnight - Discard the solution into a regular sink, and wash down with plenty = of water. Hope this is of use,=20 Best regards Emma --=20 No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.8.3/359 - Release Date: = 08/06/2006 =20 From heidgordon <@t> yahoo.com Mon Jun 12 12:24:36 2006 From: heidgordon <@t> yahoo.com (heidi gordon) Date: Mon Jun 12 12:24:41 2006 Subject: [Histonet] formalin disposal Message-ID: <20060612172436.91656.qmail@web30715.mail.mud.yahoo.com> I work at a facility that processes its own tissue. We keep the tissue blocks in a bucket of formalin until it is put on the processor at the end of the day. I have been disposing of it at the end of each day. I am wondering how often most facilities dispose of this formalin. Everyday? When it looks dirty? __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From mcauliff <@t> umdnj.edu Mon Jun 12 12:44:57 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Mon Jun 12 12:44:32 2006 Subject: [Histonet] viability question In-Reply-To: <448D8929.3000809@cs.cmu.edu> References: <448D8929.3000809@cs.cmu.edu> Message-ID: <448DA819.9080605@umdnj.edu> It depends on the storage conditions. If the tissue is stored in a physiological buffer with oxygen bubbled through it and somewhere near 37 degrees C, probably yes. If not ..........? Geoff Fawn Jones wrote: > Dear histonetters, > > I am looking for some information for a friend. She wants to find out > if her tissues (aortas) are live or not. Does anyone have any > experience performing viability stains on tissue samples? She tried > the Molecular Probes Live/Dead kit but found it insufficient due to > permeabilty issues. She also suggested MTT, but we're not sure how > well that stain works with tissues. Any help would be greatly > appreciated. > > Thank you > Fawn Jones HT(ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From gcallis <@t> montana.edu Mon Jun 12 12:57:27 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Jun 12 12:57:30 2006 Subject: Curious about this reply Re: [Histonet] RE: Disposal of Ventana DAB waste In-Reply-To: References: Message-ID: <6.0.0.22.1.20060612112719.01b43b78@gemini.msu.montana.edu> Emma, Is this what Ventana advises? Labs should check with their local waste water treatment plant before TREATING anything or putting "diluted" DAB down the drain. If laboratories are generating this kind of waste on a weekly basis, this adds up over time. In general, waste water plants do not like this and EPA does monitor water around here. Montana has discharge rules (maybe for larger industries) but our city does not want medicines, household cleaners, solvents, fertilizers, pesticides, oil, etc, flushed down the drains and warn residents frequently about this in their water bills. Wouldn't it be better to collect and have it hauled away for proper chemical disposal than add even "minute" amounts of a potential carcinogen to our water supply. We use very little DAB in our lab, but all chromogens AEC, DAB, permanent red, etc, (very low volume usage) are collected for chemical waste pickup and proper disposal. An interesting sidelight, tested water wells in parts of Montana now have traces of sunscreen chemical, medicines, herbicides, nitrates and nitrites. So much for the "pristine" environment and pure water out in the Wild Wild West /Rocky Mountain region. Drink beer and brush you teeth with it too, it may be safer when you visit some Montana dude ranch! Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 At 11:19 AM 6/12/2006, you wrote: >Hi Joe, >A specific answer to your question, regarding Ventana not DAKO waste. The >volume of DAB in the waste is very small and diluted considerably by the >other solvent free solutions. >It can be thrown down the sink, washed down with plenty of water. > >However there is a technique for deactivation of DAB which is applicable >to any form of DAB waste. >Prepare a 2 mol/litre H2SO4 solution, and a 0.2 mol/litre KMnO4 solution > - Add 50 ml of each solution to the Ventana DAB waste to be destroyed > - Let the 3 solutions react overnight > - Discard the solution into a regular sink, and wash down with > plenty of water. > >Hope this is of use, >Best regards >Emma > > >-- >No virus found in this outgoing message. >Checked by AVG Free Edition. >Version: 7.1.394 / Virus Database: 268.8.3/359 - Release Date: 08/06/2006 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AFeatherstone <@t> KaleidaHealth.Org Mon Jun 12 13:00:21 2006 From: AFeatherstone <@t> KaleidaHealth.Org (Featherstone, Annette) Date: Mon Jun 12 13:00:30 2006 Subject: [Histonet] RE: Histonet Digest, Vol 31, Issue 17 Message-ID: <9B4A77DF11463E4FB723D484214AE9BCA552B1@KALEXMB02.KaleidaHealth.org> We have been noticing a marked difference in our ER and PR's on breast tissue based on fixation. The shorter the fixation time, the lighter the staining. We of course have time restraints for turn around time and many pathologists are wanting their cases put through the same day as the surgery. Are there any thoughts out there on how to provide quicker but adequate fixation? We have tried alcoholic formalin but it takes out the inks for the margins. We have also used straight 37% formalin. Any suggestions would be greatly appreciated. Annette Featherstone -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, June 12, 2006 13:03 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 31, Issue 17 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Freezing small pieces of skeletal muscle tissue (Katri Tuomala) 2. RE:Vectastain Elite kits (Till, Renee) 3. RE: DAB disposal (Tom McNemar) 4. Tissue control for CD56 (Castillos, Luminita) 5. RE: DAB disposal (Anne Van Binsbergen) 6. CAP question on humidity levels (Joe Nocito) 7. Re: Tissue control for CD56 (Rene J Buesa) 8. RE: Cryoprotection (Alan Bright) 9. RE: CAP question on humidity levels (Dawson, Glen) 10. RE: DAB disposal (Laurie Colbert) 11. viability question (Fawn Jones) 12. RE: CAP question on humidity levels (Andrea Grantham) 13. Re: CAP question on humidity levels (Jennifer MacDonald) 14. Minimizing shrinkage (Timothy Macatee) 15. RE: Old Microtome.........knives (Edmondson David (RBV) NHS Christie Tr) 16. Re: Minimizing shrinkage (Chris Pomajzl) 17. why (Santana, Diane) 18. Re: why (Jackie M O'Connor) 19. RE: Minimizing shrinkage (Charles Scouten) ---------------------------------------------------------------------- Message: 1 Date: Sun, 11 Jun 2006 19:29:07 -0400 From: "Katri Tuomala" Subject: Re: [Histonet] Freezing small pieces of skeletal muscle tissue To: "Dearolf, Jennifer" , Message-ID: <006701c68dae$d5e7ec10$6a9a9618@Katri> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Jenn, I'm sure you'll get some good advice after the weekend from people who do this all the time. My initial thought is, that your specimen should go from isopentane directly to -70C, not -20C, unless you cut it then. When you transfer the tissue to -70C, ice ctystal artifact will form, when tissue freezes slowly from -20 to -70. Just a thought... Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "Dearolf, Jennifer" To: Sent: Friday, June 09, 2006 3:20 PM Subject: [Histonet] Freezing small pieces of skeletal muscle tissue Greetings, Histonetters, I realize that freezing skeletal muscle tissue has been a topic many times before, but in going through the archives, I could not find anyone that was using the method that we are (and maybe that's the problem, since we are having issues with freezing artifact). Thus, I am writing the list to see if anyone has any suggestions for how we might modify our methods to have better results. We collect our tissue fresh and mount it in 5% gum tragacanth on cork blocks (6mm thick). To freeze the tissue, we put some isopentane in a frozen juice can (with the juice removed, of course!) and place the juice can in liquid nitrogen. The liquid nitrogen is contained in a small, styrofoam cooler. When the isopentane gets slushy, we freeze our samples for 60 seconds. We then toss the frozen samples into a cryostat set at -21C. Once the samples have warmed up and any liquid isopentane has evaporated, we wrap our samples in parafilm and store in cardboard boxes in a -70C freezer. To cut, we move the boxes back into the cryostat and allow to wam up to -21C for approximately an hour. Then, we cut. I used this method successfully with larger pieces of muscle, but we are now attempting to freeze some mouse and guinea pig muscles (diaphragm, scalenus, rectus thoracis, and rectus abdominus), and we are getting a ton of freezing artifact. To try to prevent the artifact, we reduced the freezing time to 20 seconds. We also tried wrapping our samples in small pieces of liver. Neither method seems to work consistently. I am at my wits end. Everytime I think we have the freezing artifact beaten, it comes back with a vengence. I would appreciate any advice. I will keep track of the temperature of the isopentane, since this seems to be an important variable to control (according to the discussions in the archives). I would like to ask, however, should the temperature be -150 or -160C when you freeze the muscle? Thanks for your help! Sincerely, Jenn Jennifer Dearolf, Ph.D. Assistant Professor Biology Department Hendrix College 1600 Washington Avenue Conway, AR 72032 (501) 450-4530 (office) (501) 450-4547 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Mon, 12 Jun 2006 06:56:50 -0500 From: "Till, Renee" Subject: [Histonet] RE:Vectastain Elite kits To: histonet@lists.utsouthwestern.edu Message-ID: <11F927674DEBDC43B960809A7403C5D2012AC5F6@MAILPED.ad.uams.edu> Content-Type: text/plain; charset=us-ascii It's always worked fine for me. If I remember correctly, it does say something in the package insert about not exchanging reagents from different kits because they are optimized for that kit, but I've never had any problems. I've even used the blocking serum and the secondary from two different kits of the same species. Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72002 Office (501)364-2785 Fax (501)364-3161 Message: 1 Date: Fri, 9 Jun 2006 12:29:00 -0500 From: jhaviland@mdanderson.org Subject: [Histonet] Vectastain elite kits To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Dear Histonetters: I am hoping someone has an answer : ) I had a crazy question run through my brain. Is my thinking cap flawed today...I am using a Vectastain Elite kit that is rabbit and one that is mouse. Is the ABC reagent the same in each kit and could be used interchangeable for either kit ( then I could save on making only one) or is it animal specific? Thanks Joie ================================================================================================ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ================================================================================================ ------------------------------ Message: 3 Date: Mon, 12 Jun 2006 08:58:47 -0400 From: "Tom McNemar" Subject: RE: [Histonet] DAB disposal To: "Joe Nocito" , Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F3C9@lmhsmail.lmhealth.org> Content-Type: text/plain; charset="iso-8859-1" I have the Dako unit. It separates the hazardous/non-hazardous waste. The DAB goes into a 20L carboy that is then hauled off-site for disposal. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Joe Nocito Sent: Friday, June 09, 2006 7:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DAB disposal with the risk of sounding stupid (which happens to me often) how are people disposing their DAB contaminated Ventana waste? I mean, there is so little actual DAB present. I refuse to answer any questions that might incriminate me. I know what you are thinking. I have a new safety office who just happens to be certified in hazmat (yes, for those who know me, it's Hector). Hector keeps bugging me about it. For those who don't know us, Hector and I are joined at the hip. Last year in Ft. Lauderdale, everyone knew something was wrong because they saw Hector without me. No, we are not homosexuals, not that it matters. Hector was my histology instructor and we are really, really close friends. Oh d _ _ m, I'm sure I just offended somebody. I'm leaving now before I say something else wrong. It must be Friday. Why do I do this to myself? Have a safe weekend y'all. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Mon, 12 Jun 2006 09:12:17 -0400 From: "Castillos, Luminita" Subject: [Histonet] Tissue control for CD56 To: Message-ID: Content-Type: text/plain; charset="us-ascii" Good morning everyone, I am working with human endometrial FFPE tissue and I am doing IHC for NK using CD56 monoclonal antibody. I would like to ask which will be a good positive tissue control and a negative tissue control for CD56?. Also, if there is a vendor from where I can buy these tissue slides?. Thank you so much. Sincerely, Luminita Luminita Castillos, Ph.D. Research Scientist Cogenics(r), A Division of Clinical Data(r) Comprehensive Pharmacogenomics & Molecular Services(tm) 108 Alexader Dr. RTP, NC 27709 Tel: 919-425-2967 www.cogenics.com ------------------------------ Message: 5 Date: Mon, 12 Jun 2006 17:24:03 +0400 From: "Anne Van Binsbergen" Subject: RE: [Histonet] DAB disposal To: "Tom McNemar" , "Joe Nocito" , Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi Tom Today we became proud owners of a brand new Dako unit and I had that exact thought in mind - what to do with a 20 litre bottle of dab-contaminated waste....every couple of weeks - here in the UAE we have little choice as there are no federal laws and no off-site waste companies to cart waste off even for a fee. I am stuck until someone in authority writes the book of rules. I just know that one of you will go 'gasp!shock!horror'! and insist that I be pro-active - I am - some of you know me to be 'tenacious' - this is correct - but even that has not helped me here. Advice has been offered, best practice quoted, OH&S quoted, internet sites accessed, printed, handed over in report form, promises are made by those in authority and then broken. We stockpile safely off site and wait This is true for ALL toxic waste in this laboratory, as well as the rest of the labs, together with mercury filled blood pressure cuffs, some broken, 'expired' rat poison, old mercury thermometers, 'unknown' unlabelled chemicals from shut down labs...some scary stuff But - I digress - back to the DAB....and how to manage the growing volume of dab waste..... Any suggestions.....??? Anyone.....??? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Monday, June 12, 2006 4:59 PM To: Joe Nocito; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] DAB disposal I have the Dako unit. It separates the hazardous/non-hazardous waste. The DAB goes into a 20L carboy that is then hauled off-site for disposal. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Joe Nocito Sent: Friday, June 09, 2006 7:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DAB disposal with the risk of sounding stupid (which happens to me often) how are people disposing their DAB contaminated Ventana waste? I mean, there is so little actual DAB present. I refuse to answer any questions that might incriminate me. I know what you are thinking. I have a new safety office who just happens to be certified in hazmat (yes, for those who know me, it's Hector). Hector keeps bugging me about it. For those who don't know us, Hector and I are joined at the hip. Last year in Ft. Lauderdale, everyone knew something was wrong because they saw Hector without me. No, we are not homosexuals, not that it matters. Hector was my histology instructor and we are really, really close friends. Oh d _ _ m, I'm sure I just offended somebody. I'm leaving now before I say something else wrong. It must be Friday. Why do I do this to myself? Have a safe weekend y'all. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Mon, 12 Jun 2006 08:57:32 -0500 From: "Joe Nocito" Subject: [Histonet] CAP question on humidity levels To: "Histonetters" Message-ID: Content-Type: text/plain; charset="us-ascii" Okay, I can't stay quiet any longer. The new general checklist question asks if the temperature and humidity levels are monitored. Okay, but they don't state a range or what to do if the temp & humidity are out of range. Has any one come up with an idea? And people wonder why I have a problem with CAP. Happy Monday. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX ------------------------------ Message: 7 Date: Mon, 12 Jun 2006 07:15:33 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Tissue control for CD56 To: "Castillos, Luminita" , histonet@lists.utsouthwestern.edu Message-ID: <20060612141533.17828.qmail@web61216.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Luminita: For CD56 I used "brain" or "central nervous system" in general as a positive control.I never used any specific "negative" tissue, I just ran the negative slide without the primary Ab. The CD56 I used was from Novocastra with HIER at pH6 Hope this will help you. Ren? J. "Castillos, Luminita" wrote: Good morning everyone, I am working with human endometrial FFPE tissue and I am doing IHC for NK using CD56 monoclonal antibody. I would like to ask which will be a good positive tissue control and a negative tissue control for CD56?. Also, if there is a vendor from where I can buy these tissue slides?. Thank you so much. Sincerely, Luminita Luminita Castillos, Ph.D. Research Scientist Cogenics(r), A Division of Clinical Data(r) Comprehensive Pharmacogenomics & Molecular Services(tm) 108 Alexader Dr. RTP, NC 27709 Tel: 919-425-2967 www.cogenics.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com ------------------------------ Message: 8 Date: Mon, 12 Jun 2006 15:28:27 +0100 From: "Alan Bright" Subject: RE: [Histonet] Cryoprotection To: "zwiegers" , Message-ID: Content-Type: text/plain; charset="iso-8859-1" Dear Pierre, Brain should be sectioned at -8 to -12?C and if possible have the knife & anti-roll plate around -20?C. You are way too cold @ -30?C Liver & Kidney should be no lower than -18?C lower and you will just get dust. I hope this helps if any more info is required please get back to me. Happy sectioning III Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype User ID: dazzle0 -----Original Message----- From: zwiegers [mailto:zwiegers@interchange.ubc.ca] Sent: 10 June 2006 01:22 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryoprotection Good day all, Recently, we have sacrificed and perfused a batch of mice, and due to inattentiveness, some tissues (brain, spinal cord, liver, and kidney) were protected in 3% sucrose (instead of 30%). These were frozen and stored at -80 C for over a week. As soon as we started slicing the brain(s), we noticed that the tissue was shattering. At first we tried to reduce the temperature by keeping the tissue at - 30 C overnight. The tissue was still shattering when we tried to slice it. Now, since we used expired OCT, we assumed that this could have caused the problem. We then tried to use new OCT on a batch of mice that were not frozen yet (using 3% sucrose)and the results were the same. Fortunately, we have not lost all of our tissue and they will just be transferred to a 30% sucrose solution. Now, the question is, what can we do with the frozen tissue? Is it at all possible to thaw the OCT and transfer the organs to a 30% sucrose soltution with minimal damage to tissue integrity? Thank you so much in advance Sara Sarband Pierre Zwiegers _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 12 Jun 2006 10:23:53 -0500 From: "Dawson, Glen" Subject: RE: [Histonet] CAP question on humidity levels To: "Joe Nocito" , "Histonetters" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Joe, I posted a while ago on this same topic. It's just another regulation that some CAP shut-in came up with while hanging out in a dark room trying to justify his/her job. CAP just can't handle not coming up with at least a couple more crazy things to tack onto that CAP checklist every year. Next year I hear we'll need to note barometric pressure, solar wind levels & sunspot activity. Since they don't offer a range, I would suggest just writing your readings down in a tablet so that you can say you have them. Have Fun, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Joe Nocito Sent: Monday, June 12, 2006 7:58 AM To: Histonetters Subject: [Histonet] CAP question on humidity levels Okay, I can't stay quiet any longer. The new general checklist question asks if the temperature and humidity levels are monitored. Okay, but they don't state a range or what to do if the temp & humidity are out of range. Has any one come up with an idea? And people wonder why I have a problem with CAP. Happy Monday. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Mon, 12 Jun 2006 08:28:57 -0700 From: "Laurie Colbert" Subject: RE: [Histonet] DAB disposal To: "Joe Nocito" , "Histonet (E-mail)" Message-ID: <0BE6ADFAE4E7E04496BF21ABD346628005653BC4@EXCHANGE1.huntingtonhospital.com> Content-Type: text/plain; charset="iso-8859-1" We have our licensed hazardous waste hauler take it. I know there's not much DAB in the waste, but there's also the oily liquid coverslip that I don't want to pour down the drain. Laurie Colbert -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Friday, June 09, 2006 4:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DAB disposal with the risk of sounding stupid (which happens to me often) how are people disposing their DAB contaminated Ventana waste? I mean, there is so little actual DAB present. I refuse to answer any questions that might incriminate me. I know what you are thinking. I have a new safety office who just happens to be certified in hazmat (yes, for those who know me, it's Hector). Hector keeps bugging me about it. For those who don't know us, Hector and I are joined at the hip. Last year in Ft. Lauderdale, everyone knew something was wrong because they saw Hector without me. No, we are not homosexuals, not that it matters. Hector was my histology instructor and we are really, really close friends. Oh d _ _ m, I'm sure I just offended somebody. I'm leaving now before I say something else wrong. It must be Friday. Why do I do this to myself? Have a safe weekend y'all. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Mon, 12 Jun 2006 11:32:57 -0400 From: Fawn Jones Subject: [Histonet] viability question To: histonet@lists.utsouthwestern.edu Message-ID: <448D8929.3000809@cs.cmu.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Dear histonetters, I am looking for some information for a friend. She wants to find out if her tissues (aortas) are live or not. Does anyone have any experience performing viability stains on tissue samples? She tried the Molecular Probes Live/Dead kit but found it insufficient due to permeabilty issues. She also suggested MTT, but we're not sure how well that stain works with tissues. Any help would be greatly appreciated. Thank you Fawn Jones HT(ASCP) ------------------------------ Message: 12 Date: Mon, 12 Jun 2006 08:40:00 -0700 From: Andrea Grantham Subject: RE: [Histonet] CAP question on humidity levels To: "Dawson, Glen" , "Joe Nocito" , "Histonetters" Message-ID: <4.3.2.7.2.20060612083605.00ce3e98@algranth.inbox.email.arizona.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed I remember many years back that the lab where I worked in TX had to get a barometer in the lab as one of the outcomes of a CAP inspection and check the humidity - this wasn't in Histology but in the Chemistry lab. Maybe that section of the CAP checklist has the answer to your question. Or - maybe you could ask someone in chemistry and save yourself the work of trying to find the answer and only look it up if you have to. Andi At 10:23 AM 6/12/2006 -0500, Dawson, Glen wrote: >Joe, > >I posted a while ago on this same topic. It's just another regulation >that some CAP shut-in came up with while hanging out in a dark room trying >to justify his/her job. CAP just can't handle not coming up with at least >a couple more crazy things to tack onto that CAP checklist every >year. Next year I hear we'll need to note barometric pressure, solar wind >levels & sunspot activity. > >Since they don't offer a range, I would suggest just writing your readings >down in a tablet so that you can say you have them. > >Have Fun, > >Glen Dawson BS, HT & QIHC (ASCP) >IHC Manager >Milwaukee, WI > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Joe >Nocito >Sent: Monday, June 12, 2006 7:58 AM >To: Histonetters >Subject: [Histonet] CAP question on humidity levels > > > Okay, I can't stay quiet any longer. The new general checklist >question asks if the temperature and humidity levels are monitored. Okay, >but they don't state a range or what to do if the temp & humidity are out of >range. Has any one come up with an idea? And people wonder why I have a >problem with CAP. > >Happy Monday. > > > >Joe Nocito, BS, HT(ASCP) QIHC > >Histology Manager > >Pathology Reference Lab > >San Antonio, TX > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html ------------------------------ Message: 13 Date: Mon, 12 Jun 2006 08:58:56 -0700 From: Jennifer MacDonald Subject: Re: [Histonet] CAP question on humidity levels To: "Joe Nocito" Cc: Histonetters , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Blood bank room temperatures are critical for correct testing. This is on the general checklist, so it applies to the lab as a whole. "Joe Nocito" Sent by: histonet-bounces@lists.utsouthwestern.edu 06/12/2006 06:57 AM To "Histonetters" cc Subject [Histonet] CAP question on humidity levels Okay, I can't stay quiet any longer. The new general checklist question asks if the temperature and humidity levels are monitored. Okay, but they don't state a range or what to do if the temp & humidity are out of range. Has any one come up with an idea? And people wonder why I have a problem with CAP. Happy Monday. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Mon, 12 Jun 2006 11:50:06 -0400 From: Timothy Macatee Subject: [Histonet] Minimizing shrinkage To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII I have someone who wants to measure cardiac vessel diameter. Does anyone have an idea of the best process to avoid shrinkage of the tissue? Notice, how I made no reference to any situation comedy television shows. Tim -- Tim Macatee Research Histology Core New York University School of Medicine 550 First Ave. Department of Pathology. Medical Science Building - Room 521 New York, N.Y. 10016 (212) 263-3888 ------------------------------ Message: 15 Date: Mon, 12 Jun 2006 17:23:39 +0100 From: "Edmondson David \(RBV\) NHS Christie Tr" Subject: RE: [Histonet] Old Microtome.........knives To: "Luck, Greg D." , Message-ID: <3C83687E8F6AE04792E361ABE2D385B8418129@cht-mail2-2k.xchristie.nhs.uk> Content-Type: text/plain; charset="us-ascii" In the context of limited availability of servicing, I wonder at Cambridge rockers, they were not so flash but the worst servicing was a new piece of twine and a spring. The only rocker I know of, left over, came from a cryostat set up and has a limited cutting stroke. But..... If anyone wants a collection of Heiffer knives and various grades of Alumina then please to ask. They have not rusted away in spite of twenty years and more in sheds. Also have a couple of regular stones and a rather long one. What is the smooth creamy coloured sharpening stone called, is it from somewhere like Kansas? And "Paul springs", that control the cam that engages in the ratchet advance. Dave Manchester UK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Luck, Greg D. Sent: 09 June 2006 22:44 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Old Microtome Hello All, A group of physicians and technicians are going on a mission trip to a country in Africa in September. They approached me about acquiring an old unused microtome for them to take with them as there is no access to histology services where they are going. They have all the other equipment they need for histology services. If anyone has a microtome (functional not fancy) they would be willing to donate to this worthwhile mission I would be more than happy to pay for and facilitate the shipping of the microtome to them. Thanks, Greg Greg Luck Anatomic Path Spvr Deaconess Med Cntr 800 W. 5th Ave Spokane, WA 99204 Offc 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************** This e-mail and any files transmitted with it are confidential and solely for the use of the intended recipient. If you have received this e-mail in error you should not disseminate, distribute or copy it. Please notify the sender immediately and delete this e-mail from your system. ******************************************************************************************************************* ------------------------------ Message: 16 Date: Mon, 12 Jun 2006 11:38:38 -0500 From: "Chris Pomajzl" Subject: Re: [Histonet] Minimizing shrinkage To: "HISTONET" Message-ID: <008801c68e3e$a8394580$26fca8c0@CSP> Content-Type: text/plain; charset="iso-8859-1" Don't put them in the pool. (sorry, had to say it) We used to infuse vessels with a plastic polymer. Perhaps if you do this, you could measure the diameter of the plastic assuming it would not shrink. Also, what type of animal are we talking about? ----- Original Message ----- From: "Timothy Macatee" To: Sent: Monday, June 12, 2006 10:50 AM Subject: [Histonet] Minimizing shrinkage > I have someone who wants to measure cardiac vessel diameter. Does anyone > have an idea of the best process to avoid shrinkage of the tissue? Notice, > how I made no reference to any situation comedy television shows. > > Tim > -- > Tim Macatee > Research Histology Core > New York University School of Medicine > 550 First Ave. > Department of Pathology. > Medical Science Building - Room 521 > New York, N.Y. 10016 > (212) 263-3888 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Mon, 12 Jun 2006 12:40:25 -0400 From: "Santana, Diane" Subject: [Histonet] why To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <4C96AA62BADFD81180CB0002A5AD67FB07113A3E@MAILPMA> Content-Type: text/plain; charset="iso-8859-1" Hi I am looking for some ideas to help with a problem I am still having with processing my GI bx's. What would cause my tissue to be raw? Not all, not some, only 1 or 2 pieces. Example if I have a case with 4 bx's, maybe only 1 is raw, the other 3 are beautiful. All my solutions test at the right % and temperatures are fine. Question again, why does 1 piece of tissue come out raw, when the other pieces are fine? thanks in advance, Diane Santana PMA Haverhill, Mass. ------------------------------ Message: 18 Date: Mon, 12 Jun 2006 11:50:24 -0500 From: "Jackie M O'Connor" Subject: Re: [Histonet] why To: "Santana, Diane" Cc: "'histonet@lists.utsouthwestern.edu'" , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" By raw I assume you mean "underprocesssed" (which isn't even a word) Anyway - do you use biopsy pads for processing? Jackie O' "Santana, Diane" Sent by: histonet-bounces@lists.utsouthwestern.edu 06/12/2006 11:40 AM To "'histonet@lists.utsouthwestern.edu'" cc Subject [Histonet] why Hi I am looking for some ideas to help with a problem I am still having with processing my GI bx's. What would cause my tissue to be raw? Not all, not some, only 1 or 2 pieces. Example if I have a case with 4 bx's, maybe only 1 is raw, the other 3 are beautiful. All my solutions test at the right % and temperatures are fine. Question again, why does 1 piece of tissue come out raw, when the other pieces are fine? thanks in advance, Diane Santana PMA Haverhill, Mass. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Mon, 12 Jun 2006 11:56:24 -0500 From: "Charles Scouten" Subject: RE: [Histonet] Minimizing shrinkage To: "Chris Pomajzl" , "HISTONET" Message-ID: <5784D843593D874C93E9BADCB87342AB013070CD@tpiserver03.Coretech-holdings.com> Content-Type: text/plain; charset="us-ascii" See the article in Microscopy Today. Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chris Pomajzl Sent: Monday, June 12, 2006 11:39 AM To: HISTONET Subject: Re: [Histonet] Minimizing shrinkage Don't put them in the pool. (sorry, had to say it) We used to infuse vessels with a plastic polymer. Perhaps if you do this, you could measure the diameter of the plastic assuming it would not shrink. Also, what type of animal are we talking about? ----- Original Message ----- From: "Timothy Macatee" To: Sent: Monday, June 12, 2006 10:50 AM Subject: [Histonet] Minimizing shrinkage > I have someone who wants to measure cardiac vessel diameter. Does anyone > have an idea of the best process to avoid shrinkage of the tissue? Notice, > how I made no reference to any situation comedy television shows. > > Tim > -- > Tim Macatee > Research Histology Core > New York University School of Medicine > 550 First Ave. > Department of Pathology. > Medical Science Building - Room 521 > New York, N.Y. 10016 > (212) 263-3888 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 31, Issue 17 **************************************** CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. 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From bhewlett <@t> cogeco.ca Mon Jun 12 13:11:16 2006 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Mon Jun 12 13:11:24 2006 Subject: [Histonet] Old Microtome.........knives References: <3C83687E8F6AE04792E361ABE2D385B8418129@cht-mail2-2k.xchristie.nhs.uk> Message-ID: <000701c68e4b$99be3a30$6500a8c0@mainbox> Dave, Ahh, Cambridge rockers, simple but potentially dangerous piece of kit. We used piano wire not twine! The creamy coloured stone sounds like an 'Arkansaw' stone, and that's PAWL springs. Bryan ----- Original Message ----- From: "Edmondson David (RBV) NHS Christie Tr" To: "Luck, Greg D." ; Sent: Monday, June 12, 2006 12:23 PM Subject: RE: [Histonet] Old Microtome.........knives In the context of limited availability of servicing, I wonder at Cambridge rockers, they were not so flash but the worst servicing was a new piece of twine and a spring. The only rocker I know of, left over, came from a cryostat set up and has a limited cutting stroke. But..... If anyone wants a collection of Heiffer knives and various grades of Alumina then please to ask. They have not rusted away in spite of twenty years and more in sheds. Also have a couple of regular stones and a rather long one. What is the smooth creamy coloured sharpening stone called, is it from somewhere like Kansas? And "Paul springs", that control the cam that engages in the ratchet advance. Dave Manchester UK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Luck, Greg D. Sent: 09 June 2006 22:44 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Old Microtome Hello All, A group of physicians and technicians are going on a mission trip to a country in Africa in September. They approached me about acquiring an old unused microtome for them to take with them as there is no access to histology services where they are going. They have all the other equipment they need for histology services. If anyone has a microtome (functional not fancy) they would be willing to donate to this worthwhile mission I would be more than happy to pay for and facilitate the shipping of the microtome to them. Thanks, Greg Greg Luck Anatomic Path Spvr Deaconess Med Cntr 800 W. 5th Ave Spokane, WA 99204 Offc 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************** This e-mail and any files transmitted with it are confidential and solely for the use of the intended recipient. If you have received this e-mail in error you should not disseminate, distribute or copy it. Please notify the sender immediately and delete this e-mail from your system. ******************************************************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AFeatherstone <@t> KaleidaHealth.Org Mon Jun 12 13:19:38 2006 From: AFeatherstone <@t> KaleidaHealth.Org (Featherstone, Annette) Date: Mon Jun 12 13:19:47 2006 Subject: [Histonet] RE: Histonet Digest, Vol 31, Issue 17 Message-ID: <9B4A77DF11463E4FB723D484214AE9BCA552B2@KALEXMB02.KaleidaHealth.org> We have our DAB waste disposed of by Safety Kleen. Annette Featherstone -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, June 12, 2006 13:03 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 31, Issue 17 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Freezing small pieces of skeletal muscle tissue (Katri Tuomala) 2. RE:Vectastain Elite kits (Till, Renee) 3. RE: DAB disposal (Tom McNemar) 4. Tissue control for CD56 (Castillos, Luminita) 5. RE: DAB disposal (Anne Van Binsbergen) 6. CAP question on humidity levels (Joe Nocito) 7. Re: Tissue control for CD56 (Rene J Buesa) 8. RE: Cryoprotection (Alan Bright) 9. RE: CAP question on humidity levels (Dawson, Glen) 10. RE: DAB disposal (Laurie Colbert) 11. viability question (Fawn Jones) 12. RE: CAP question on humidity levels (Andrea Grantham) 13. Re: CAP question on humidity levels (Jennifer MacDonald) 14. Minimizing shrinkage (Timothy Macatee) 15. RE: Old Microtome.........knives (Edmondson David (RBV) NHS Christie Tr) 16. Re: Minimizing shrinkage (Chris Pomajzl) 17. why (Santana, Diane) 18. Re: why (Jackie M O'Connor) 19. RE: Minimizing shrinkage (Charles Scouten) ---------------------------------------------------------------------- Message: 1 Date: Sun, 11 Jun 2006 19:29:07 -0400 From: "Katri Tuomala" Subject: Re: [Histonet] Freezing small pieces of skeletal muscle tissue To: "Dearolf, Jennifer" , Message-ID: <006701c68dae$d5e7ec10$6a9a9618@Katri> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Jenn, I'm sure you'll get some good advice after the weekend from people who do this all the time. My initial thought is, that your specimen should go from isopentane directly to -70C, not -20C, unless you cut it then. When you transfer the tissue to -70C, ice ctystal artifact will form, when tissue freezes slowly from -20 to -70. Just a thought... Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "Dearolf, Jennifer" To: Sent: Friday, June 09, 2006 3:20 PM Subject: [Histonet] Freezing small pieces of skeletal muscle tissue Greetings, Histonetters, I realize that freezing skeletal muscle tissue has been a topic many times before, but in going through the archives, I could not find anyone that was using the method that we are (and maybe that's the problem, since we are having issues with freezing artifact). Thus, I am writing the list to see if anyone has any suggestions for how we might modify our methods to have better results. We collect our tissue fresh and mount it in 5% gum tragacanth on cork blocks (6mm thick). To freeze the tissue, we put some isopentane in a frozen juice can (with the juice removed, of course!) and place the juice can in liquid nitrogen. The liquid nitrogen is contained in a small, styrofoam cooler. When the isopentane gets slushy, we freeze our samples for 60 seconds. We then toss the frozen samples into a cryostat set at -21C. Once the samples have warmed up and any liquid isopentane has evaporated, we wrap our samples in parafilm and store in cardboard boxes in a -70C freezer. To cut, we move the boxes back into the cryostat and allow to wam up to -21C for approximately an hour. Then, we cut. I used this method successfully with larger pieces of muscle, but we are now attempting to freeze some mouse and guinea pig muscles (diaphragm, scalenus, rectus thoracis, and rectus abdominus), and we are getting a ton of freezing artifact. To try to prevent the artifact, we reduced the freezing time to 20 seconds. We also tried wrapping our samples in small pieces of liver. Neither method seems to work consistently. I am at my wits end. Everytime I think we have the freezing artifact beaten, it comes back with a vengence. I would appreciate any advice. I will keep track of the temperature of the isopentane, since this seems to be an important variable to control (according to the discussions in the archives). I would like to ask, however, should the temperature be -150 or -160C when you freeze the muscle? Thanks for your help! Sincerely, Jenn Jennifer Dearolf, Ph.D. Assistant Professor Biology Department Hendrix College 1600 Washington Avenue Conway, AR 72032 (501) 450-4530 (office) (501) 450-4547 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Mon, 12 Jun 2006 06:56:50 -0500 From: "Till, Renee" Subject: [Histonet] RE:Vectastain Elite kits To: histonet@lists.utsouthwestern.edu Message-ID: <11F927674DEBDC43B960809A7403C5D2012AC5F6@MAILPED.ad.uams.edu> Content-Type: text/plain; charset=us-ascii It's always worked fine for me. If I remember correctly, it does say something in the package insert about not exchanging reagents from different kits because they are optimized for that kit, but I've never had any problems. I've even used the blocking serum and the secondary from two different kits of the same species. Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72002 Office (501)364-2785 Fax (501)364-3161 Message: 1 Date: Fri, 9 Jun 2006 12:29:00 -0500 From: jhaviland@mdanderson.org Subject: [Histonet] Vectastain elite kits To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Dear Histonetters: I am hoping someone has an answer : ) I had a crazy question run through my brain. Is my thinking cap flawed today...I am using a Vectastain Elite kit that is rabbit and one that is mouse. Is the ABC reagent the same in each kit and could be used interchangeable for either kit ( then I could save on making only one) or is it animal specific? Thanks Joie ================================================================================================ Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ================================================================================================ ------------------------------ Message: 3 Date: Mon, 12 Jun 2006 08:58:47 -0400 From: "Tom McNemar" Subject: RE: [Histonet] DAB disposal To: "Joe Nocito" , Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F3C9@lmhsmail.lmhealth.org> Content-Type: text/plain; charset="iso-8859-1" I have the Dako unit. It separates the hazardous/non-hazardous waste. The DAB goes into a 20L carboy that is then hauled off-site for disposal. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Joe Nocito Sent: Friday, June 09, 2006 7:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DAB disposal with the risk of sounding stupid (which happens to me often) how are people disposing their DAB contaminated Ventana waste? I mean, there is so little actual DAB present. I refuse to answer any questions that might incriminate me. I know what you are thinking. I have a new safety office who just happens to be certified in hazmat (yes, for those who know me, it's Hector). Hector keeps bugging me about it. For those who don't know us, Hector and I are joined at the hip. Last year in Ft. Lauderdale, everyone knew something was wrong because they saw Hector without me. No, we are not homosexuals, not that it matters. Hector was my histology instructor and we are really, really close friends. Oh d _ _ m, I'm sure I just offended somebody. I'm leaving now before I say something else wrong. It must be Friday. Why do I do this to myself? Have a safe weekend y'all. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 4 Date: Mon, 12 Jun 2006 09:12:17 -0400 From: "Castillos, Luminita" Subject: [Histonet] Tissue control for CD56 To: Message-ID: Content-Type: text/plain; charset="us-ascii" Good morning everyone, I am working with human endometrial FFPE tissue and I am doing IHC for NK using CD56 monoclonal antibody. I would like to ask which will be a good positive tissue control and a negative tissue control for CD56?. Also, if there is a vendor from where I can buy these tissue slides?. Thank you so much. Sincerely, Luminita Luminita Castillos, Ph.D. Research Scientist Cogenics(r), A Division of Clinical Data(r) Comprehensive Pharmacogenomics & Molecular Services(tm) 108 Alexader Dr. RTP, NC 27709 Tel: 919-425-2967 www.cogenics.com ------------------------------ Message: 5 Date: Mon, 12 Jun 2006 17:24:03 +0400 From: "Anne Van Binsbergen" Subject: RE: [Histonet] DAB disposal To: "Tom McNemar" , "Joe Nocito" , Message-ID: Content-Type: text/plain; charset="US-ASCII" Hi Tom Today we became proud owners of a brand new Dako unit and I had that exact thought in mind - what to do with a 20 litre bottle of dab-contaminated waste....every couple of weeks - here in the UAE we have little choice as there are no federal laws and no off-site waste companies to cart waste off even for a fee. I am stuck until someone in authority writes the book of rules. I just know that one of you will go 'gasp!shock!horror'! and insist that I be pro-active - I am - some of you know me to be 'tenacious' - this is correct - but even that has not helped me here. Advice has been offered, best practice quoted, OH&S quoted, internet sites accessed, printed, handed over in report form, promises are made by those in authority and then broken. We stockpile safely off site and wait This is true for ALL toxic waste in this laboratory, as well as the rest of the labs, together with mercury filled blood pressure cuffs, some broken, 'expired' rat poison, old mercury thermometers, 'unknown' unlabelled chemicals from shut down labs...some scary stuff But - I digress - back to the DAB....and how to manage the growing volume of dab waste..... Any suggestions.....??? Anyone.....??? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Monday, June 12, 2006 4:59 PM To: Joe Nocito; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] DAB disposal I have the Dako unit. It separates the hazardous/non-hazardous waste. The DAB goes into a 20L carboy that is then hauled off-site for disposal. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Joe Nocito Sent: Friday, June 09, 2006 7:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DAB disposal with the risk of sounding stupid (which happens to me often) how are people disposing their DAB contaminated Ventana waste? I mean, there is so little actual DAB present. I refuse to answer any questions that might incriminate me. I know what you are thinking. I have a new safety office who just happens to be certified in hazmat (yes, for those who know me, it's Hector). Hector keeps bugging me about it. For those who don't know us, Hector and I are joined at the hip. Last year in Ft. Lauderdale, everyone knew something was wrong because they saw Hector without me. No, we are not homosexuals, not that it matters. Hector was my histology instructor and we are really, really close friends. Oh d _ _ m, I'm sure I just offended somebody. I'm leaving now before I say something else wrong. It must be Friday. Why do I do this to myself? Have a safe weekend y'all. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Mon, 12 Jun 2006 08:57:32 -0500 From: "Joe Nocito" Subject: [Histonet] CAP question on humidity levels To: "Histonetters" Message-ID: Content-Type: text/plain; charset="us-ascii" Okay, I can't stay quiet any longer. The new general checklist question asks if the temperature and humidity levels are monitored. Okay, but they don't state a range or what to do if the temp & humidity are out of range. Has any one come up with an idea? And people wonder why I have a problem with CAP. Happy Monday. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX ------------------------------ Message: 7 Date: Mon, 12 Jun 2006 07:15:33 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Tissue control for CD56 To: "Castillos, Luminita" , histonet@lists.utsouthwestern.edu Message-ID: <20060612141533.17828.qmail@web61216.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Luminita: For CD56 I used "brain" or "central nervous system" in general as a positive control.I never used any specific "negative" tissue, I just ran the negative slide without the primary Ab. The CD56 I used was from Novocastra with HIER at pH6 Hope this will help you. Ren? J. "Castillos, Luminita" wrote: Good morning everyone, I am working with human endometrial FFPE tissue and I am doing IHC for NK using CD56 monoclonal antibody. I would like to ask which will be a good positive tissue control and a negative tissue control for CD56?. Also, if there is a vendor from where I can buy these tissue slides?. Thank you so much. Sincerely, Luminita Luminita Castillos, Ph.D. Research Scientist Cogenics(r), A Division of Clinical Data(r) Comprehensive Pharmacogenomics & Molecular Services(tm) 108 Alexader Dr. RTP, NC 27709 Tel: 919-425-2967 www.cogenics.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com ------------------------------ Message: 8 Date: Mon, 12 Jun 2006 15:28:27 +0100 From: "Alan Bright" Subject: RE: [Histonet] Cryoprotection To: "zwiegers" , Message-ID: Content-Type: text/plain; charset="iso-8859-1" Dear Pierre, Brain should be sectioned at -8 to -12?C and if possible have the knife & anti-roll plate around -20?C. You are way too cold @ -30?C Liver & Kidney should be no lower than -18?C lower and you will just get dust. I hope this helps if any more info is required please get back to me. Happy sectioning III Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype User ID: dazzle0 -----Original Message----- From: zwiegers [mailto:zwiegers@interchange.ubc.ca] Sent: 10 June 2006 01:22 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cryoprotection Good day all, Recently, we have sacrificed and perfused a batch of mice, and due to inattentiveness, some tissues (brain, spinal cord, liver, and kidney) were protected in 3% sucrose (instead of 30%). These were frozen and stored at -80 C for over a week. As soon as we started slicing the brain(s), we noticed that the tissue was shattering. At first we tried to reduce the temperature by keeping the tissue at - 30 C overnight. The tissue was still shattering when we tried to slice it. Now, since we used expired OCT, we assumed that this could have caused the problem. We then tried to use new OCT on a batch of mice that were not frozen yet (using 3% sucrose)and the results were the same. Fortunately, we have not lost all of our tissue and they will just be transferred to a 30% sucrose solution. Now, the question is, what can we do with the frozen tissue? Is it at all possible to thaw the OCT and transfer the organs to a 30% sucrose soltution with minimal damage to tissue integrity? Thank you so much in advance Sara Sarband Pierre Zwiegers _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 12 Jun 2006 10:23:53 -0500 From: "Dawson, Glen" Subject: RE: [Histonet] CAP question on humidity levels To: "Joe Nocito" , "Histonetters" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Joe, I posted a while ago on this same topic. It's just another regulation that some CAP shut-in came up with while hanging out in a dark room trying to justify his/her job. CAP just can't handle not coming up with at least a couple more crazy things to tack onto that CAP checklist every year. Next year I hear we'll need to note barometric pressure, solar wind levels & sunspot activity. Since they don't offer a range, I would suggest just writing your readings down in a tablet so that you can say you have them. Have Fun, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Joe Nocito Sent: Monday, June 12, 2006 7:58 AM To: Histonetters Subject: [Histonet] CAP question on humidity levels Okay, I can't stay quiet any longer. The new general checklist question asks if the temperature and humidity levels are monitored. Okay, but they don't state a range or what to do if the temp & humidity are out of range. Has any one come up with an idea? And people wonder why I have a problem with CAP. Happy Monday. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Mon, 12 Jun 2006 08:28:57 -0700 From: "Laurie Colbert" Subject: RE: [Histonet] DAB disposal To: "Joe Nocito" , "Histonet (E-mail)" Message-ID: <0BE6ADFAE4E7E04496BF21ABD346628005653BC4@EXCHANGE1.huntingtonhospital.com> Content-Type: text/plain; charset="iso-8859-1" We have our licensed hazardous waste hauler take it. I know there's not much DAB in the waste, but there's also the oily liquid coverslip that I don't want to pour down the drain. Laurie Colbert -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Friday, June 09, 2006 4:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DAB disposal with the risk of sounding stupid (which happens to me often) how are people disposing their DAB contaminated Ventana waste? I mean, there is so little actual DAB present. I refuse to answer any questions that might incriminate me. I know what you are thinking. I have a new safety office who just happens to be certified in hazmat (yes, for those who know me, it's Hector). Hector keeps bugging me about it. For those who don't know us, Hector and I are joined at the hip. Last year in Ft. Lauderdale, everyone knew something was wrong because they saw Hector without me. No, we are not homosexuals, not that it matters. Hector was my histology instructor and we are really, really close friends. Oh d _ _ m, I'm sure I just offended somebody. I'm leaving now before I say something else wrong. It must be Friday. Why do I do this to myself? Have a safe weekend y'all. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Mon, 12 Jun 2006 11:32:57 -0400 From: Fawn Jones Subject: [Histonet] viability question To: histonet@lists.utsouthwestern.edu Message-ID: <448D8929.3000809@cs.cmu.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Dear histonetters, I am looking for some information for a friend. She wants to find out if her tissues (aortas) are live or not. Does anyone have any experience performing viability stains on tissue samples? She tried the Molecular Probes Live/Dead kit but found it insufficient due to permeabilty issues. She also suggested MTT, but we're not sure how well that stain works with tissues. Any help would be greatly appreciated. Thank you Fawn Jones HT(ASCP) ------------------------------ Message: 12 Date: Mon, 12 Jun 2006 08:40:00 -0700 From: Andrea Grantham Subject: RE: [Histonet] CAP question on humidity levels To: "Dawson, Glen" , "Joe Nocito" , "Histonetters" Message-ID: <4.3.2.7.2.20060612083605.00ce3e98@algranth.inbox.email.arizona.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed I remember many years back that the lab where I worked in TX had to get a barometer in the lab as one of the outcomes of a CAP inspection and check the humidity - this wasn't in Histology but in the Chemistry lab. Maybe that section of the CAP checklist has the answer to your question. Or - maybe you could ask someone in chemistry and save yourself the work of trying to find the answer and only look it up if you have to. Andi At 10:23 AM 6/12/2006 -0500, Dawson, Glen wrote: >Joe, > >I posted a while ago on this same topic. It's just another regulation >that some CAP shut-in came up with while hanging out in a dark room trying >to justify his/her job. CAP just can't handle not coming up with at least >a couple more crazy things to tack onto that CAP checklist every >year. Next year I hear we'll need to note barometric pressure, solar wind >levels & sunspot activity. > >Since they don't offer a range, I would suggest just writing your readings >down in a tablet so that you can say you have them. > >Have Fun, > >Glen Dawson BS, HT & QIHC (ASCP) >IHC Manager >Milwaukee, WI > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Joe >Nocito >Sent: Monday, June 12, 2006 7:58 AM >To: Histonetters >Subject: [Histonet] CAP question on humidity levels > > > Okay, I can't stay quiet any longer. The new general checklist >question asks if the temperature and humidity levels are monitored. Okay, >but they don't state a range or what to do if the temp & humidity are out of >range. Has any one come up with an idea? And people wonder why I have a >problem with CAP. > >Happy Monday. > > > >Joe Nocito, BS, HT(ASCP) QIHC > >Histology Manager > >Pathology Reference Lab > >San Antonio, TX > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html ------------------------------ Message: 13 Date: Mon, 12 Jun 2006 08:58:56 -0700 From: Jennifer MacDonald Subject: Re: [Histonet] CAP question on humidity levels To: "Joe Nocito" Cc: Histonetters , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Blood bank room temperatures are critical for correct testing. This is on the general checklist, so it applies to the lab as a whole. "Joe Nocito" Sent by: histonet-bounces@lists.utsouthwestern.edu 06/12/2006 06:57 AM To "Histonetters" cc Subject [Histonet] CAP question on humidity levels Okay, I can't stay quiet any longer. The new general checklist question asks if the temperature and humidity levels are monitored. Okay, but they don't state a range or what to do if the temp & humidity are out of range. Has any one come up with an idea? And people wonder why I have a problem with CAP. Happy Monday. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Mon, 12 Jun 2006 11:50:06 -0400 From: Timothy Macatee Subject: [Histonet] Minimizing shrinkage To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII I have someone who wants to measure cardiac vessel diameter. Does anyone have an idea of the best process to avoid shrinkage of the tissue? Notice, how I made no reference to any situation comedy television shows. Tim -- Tim Macatee Research Histology Core New York University School of Medicine 550 First Ave. Department of Pathology. Medical Science Building - Room 521 New York, N.Y. 10016 (212) 263-3888 ------------------------------ Message: 15 Date: Mon, 12 Jun 2006 17:23:39 +0100 From: "Edmondson David \(RBV\) NHS Christie Tr" Subject: RE: [Histonet] Old Microtome.........knives To: "Luck, Greg D." , Message-ID: <3C83687E8F6AE04792E361ABE2D385B8418129@cht-mail2-2k.xchristie.nhs.uk> Content-Type: text/plain; charset="us-ascii" In the context of limited availability of servicing, I wonder at Cambridge rockers, they were not so flash but the worst servicing was a new piece of twine and a spring. The only rocker I know of, left over, came from a cryostat set up and has a limited cutting stroke. But..... If anyone wants a collection of Heiffer knives and various grades of Alumina then please to ask. They have not rusted away in spite of twenty years and more in sheds. Also have a couple of regular stones and a rather long one. What is the smooth creamy coloured sharpening stone called, is it from somewhere like Kansas? And "Paul springs", that control the cam that engages in the ratchet advance. Dave Manchester UK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Luck, Greg D. Sent: 09 June 2006 22:44 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Old Microtome Hello All, A group of physicians and technicians are going on a mission trip to a country in Africa in September. They approached me about acquiring an old unused microtome for them to take with them as there is no access to histology services where they are going. They have all the other equipment they need for histology services. If anyone has a microtome (functional not fancy) they would be willing to donate to this worthwhile mission I would be more than happy to pay for and facilitate the shipping of the microtome to them. Thanks, Greg Greg Luck Anatomic Path Spvr Deaconess Med Cntr 800 W. 5th Ave Spokane, WA 99204 Offc 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************** This e-mail and any files transmitted with it are confidential and solely for the use of the intended recipient. If you have received this e-mail in error you should not disseminate, distribute or copy it. Please notify the sender immediately and delete this e-mail from your system. ******************************************************************************************************************* ------------------------------ Message: 16 Date: Mon, 12 Jun 2006 11:38:38 -0500 From: "Chris Pomajzl" Subject: Re: [Histonet] Minimizing shrinkage To: "HISTONET" Message-ID: <008801c68e3e$a8394580$26fca8c0@CSP> Content-Type: text/plain; charset="iso-8859-1" Don't put them in the pool. (sorry, had to say it) We used to infuse vessels with a plastic polymer. Perhaps if you do this, you could measure the diameter of the plastic assuming it would not shrink. Also, what type of animal are we talking about? ----- Original Message ----- From: "Timothy Macatee" To: Sent: Monday, June 12, 2006 10:50 AM Subject: [Histonet] Minimizing shrinkage > I have someone who wants to measure cardiac vessel diameter. Does anyone > have an idea of the best process to avoid shrinkage of the tissue? Notice, > how I made no reference to any situation comedy television shows. > > Tim > -- > Tim Macatee > Research Histology Core > New York University School of Medicine > 550 First Ave. > Department of Pathology. > Medical Science Building - Room 521 > New York, N.Y. 10016 > (212) 263-3888 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Mon, 12 Jun 2006 12:40:25 -0400 From: "Santana, Diane" Subject: [Histonet] why To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <4C96AA62BADFD81180CB0002A5AD67FB07113A3E@MAILPMA> Content-Type: text/plain; charset="iso-8859-1" Hi I am looking for some ideas to help with a problem I am still having with processing my GI bx's. What would cause my tissue to be raw? Not all, not some, only 1 or 2 pieces. Example if I have a case with 4 bx's, maybe only 1 is raw, the other 3 are beautiful. All my solutions test at the right % and temperatures are fine. Question again, why does 1 piece of tissue come out raw, when the other pieces are fine? thanks in advance, Diane Santana PMA Haverhill, Mass. ------------------------------ Message: 18 Date: Mon, 12 Jun 2006 11:50:24 -0500 From: "Jackie M O'Connor" Subject: Re: [Histonet] why To: "Santana, Diane" Cc: "'histonet@lists.utsouthwestern.edu'" , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" By raw I assume you mean "underprocesssed" (which isn't even a word) Anyway - do you use biopsy pads for processing? Jackie O' "Santana, Diane" Sent by: histonet-bounces@lists.utsouthwestern.edu 06/12/2006 11:40 AM To "'histonet@lists.utsouthwestern.edu'" cc Subject [Histonet] why Hi I am looking for some ideas to help with a problem I am still having with processing my GI bx's. What would cause my tissue to be raw? Not all, not some, only 1 or 2 pieces. Example if I have a case with 4 bx's, maybe only 1 is raw, the other 3 are beautiful. All my solutions test at the right % and temperatures are fine. Question again, why does 1 piece of tissue come out raw, when the other pieces are fine? thanks in advance, Diane Santana PMA Haverhill, Mass. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 19 Date: Mon, 12 Jun 2006 11:56:24 -0500 From: "Charles Scouten" Subject: RE: [Histonet] Minimizing shrinkage To: "Chris Pomajzl" , "HISTONET" Message-ID: <5784D843593D874C93E9BADCB87342AB013070CD@tpiserver03.Coretech-holdings.com> Content-Type: text/plain; charset="us-ascii" See the article in Microscopy Today. Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chris Pomajzl Sent: Monday, June 12, 2006 11:39 AM To: HISTONET Subject: Re: [Histonet] Minimizing shrinkage Don't put them in the pool. (sorry, had to say it) We used to infuse vessels with a plastic polymer. Perhaps if you do this, you could measure the diameter of the plastic assuming it would not shrink. Also, what type of animal are we talking about? ----- Original Message ----- From: "Timothy Macatee" To: Sent: Monday, June 12, 2006 10:50 AM Subject: [Histonet] Minimizing shrinkage > I have someone who wants to measure cardiac vessel diameter. Does anyone > have an idea of the best process to avoid shrinkage of the tissue? Notice, > how I made no reference to any situation comedy television shows. > > Tim > -- > Tim Macatee > Research Histology Core > New York University School of Medicine > 550 First Ave. > Department of Pathology. > Medical Science Building - Room 521 > New York, N.Y. 10016 > (212) 263-3888 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 31, Issue 17 **************************************** CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From Mary.Vaughan <@t> RoswellPark.org Mon Jun 12 13:31:31 2006 From: Mary.Vaughan <@t> RoswellPark.org (Vaughan, Mary) Date: Mon Jun 12 13:31:09 2006 Subject: [Histonet] core service labs Message-ID: <6FF91AE4F1DC7743A6466E334EB865AE0BE0F29C@VERITY.roswellpark.org> Greetings Everyone- At the recent Region I Meeting/Symposium a colleague Dr. Luis Chiriboga and I were discussing core service labs. We both work at Cancer centers and had a lot to talk about. We would be interested in setting up a group for people that work in ANY type of core lab. By core lab we mean one that provides, at a cost, routine and experimental histopathology services for non-clinical based users. We're in the process of compiling an agenda of items/issues that we feel are very important to address. We do understand that in most cases, those of us in core labs are very busy, so this won't take a lot of your time. At this point we ask only that you identify yourselves to us [PLEASE reply to mary.vaughan@roswellpark.org -not to the Histonet] and we'll develop the group list. We'll then ask for thoughts and suggestions for the list of agenda items. Thank you all for your time. Best Regards, Mary Vaughan HT (ASCP) Research Specialist Roswell Park Cancer Institute ASB-212 Elm & Carlton Streets Buffalo, N Y 14263 phone: 716.845.3006 FAX: 716.845.3489 and Luis Chiriboga Ph.D. NYU Cancer Institute and Bellevue Hospital Center New York University School Of Medicine Department Of Pathology 4W27 462 First Avenue New York, N.Y. 10016 W(212) 562-4667. F(212) 263-2041 This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. From HornHV <@t> archildrens.org Mon Jun 12 13:36:27 2006 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon Jun 12 13:37:08 2006 Subject: Curious about this reply Re: [Histonet] RE: Disposal of Ventana DAB waste Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6C07@EMAIL.archildrens.org> I agree with Gayle. I don't think it's wise to put in the drain. Our water department will not let ANY amount of DAB go down the drain no matter how small the quantity. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: Monday, June 12, 2006 12:57 PM To: Emma JONES; Histonet@lists.utsouthwestern.edu Subject: Curious about this reply Re: [Histonet] RE: Disposal of Ventana DAB waste Emma, Is this what Ventana advises? Labs should check with their local waste water treatment plant before TREATING anything or putting "diluted" DAB down the drain. If laboratories are generating this kind of waste on a weekly basis, this adds up over time. In general, waste water plants do not like this and EPA does monitor water around here. Montana has discharge rules (maybe for larger industries) but our city does not want medicines, household cleaners, solvents, fertilizers, pesticides, oil, etc, flushed down the drains and warn residents frequently about this in their water bills. Wouldn't it be better to collect and have it hauled away for proper chemical disposal than add even "minute" amounts of a potential carcinogen to our water supply. We use very little DAB in our lab, but all chromogens AEC, DAB, permanent red, etc, (very low volume usage) are collected for chemical waste pickup and proper disposal. An interesting sidelight, tested water wells in parts of Montana now have traces of sunscreen chemical, medicines, herbicides, nitrates and nitrites. So much for the "pristine" environment and pure water out in the Wild Wild West /Rocky Mountain region. Drink beer and brush you teeth with it too, it may be safer when you visit some Montana dude ranch! Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 At 11:19 AM 6/12/2006, you wrote: >Hi Joe, >A specific answer to your question, regarding Ventana not DAKO waste. The >volume of DAB in the waste is very small and diluted considerably by the >other solvent free solutions. >It can be thrown down the sink, washed down with plenty of water. > >However there is a technique for deactivation of DAB which is applicable >to any form of DAB waste. >Prepare a 2 mol/litre H2SO4 solution, and a 0.2 mol/litre KMnO4 solution > - Add 50 ml of each solution to the Ventana DAB waste to be destroyed > - Let the 3 solutions react overnight > - Discard the solution into a regular sink, and wash down with > plenty of water. > >Hope this is of use, >Best regards >Emma > > >-- >No virus found in this outgoing message. >Checked by AVG Free Edition. >Version: 7.1.394 / Virus Database: 268.8.3/359 - Release Date: 08/06/2006 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From Rcartun <@t> harthosp.org Mon Jun 12 13:38:24 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Mon Jun 12 13:39:09 2006 Subject: [Histonet] ER - fixation Message-ID: <448D7C6002000077000006AD@hcnwgwds01.hh.chs> Hello Annette: I could not reply to your original e-mail. Regarding ER and fixation, see Goldstein NS, et al.: Minimum formalin fixation time for consistent ER IHC staining of invasive breast carcinoma. Am J Clin Pathol 2003;120:86-92. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From PatPatterson <@t> mhd.com Mon Jun 12 13:41:49 2006 From: PatPatterson <@t> mhd.com (Patterson, Pat) Date: Mon Jun 12 13:41:17 2006 Subject: [Histonet] RE: Histonet - Screened cassettes Message-ID: <293C7C19EFF7D611AE1A0002A53F8114164AC283@omega.mhd.com> Teri and Diane- We have noticed the same issue you both have mentioned. We stopped using the screened cassettes and now have stopped using the all plastic Micro Cassettes. We have great biopsies for days then 1 or 2 a day would be unprocessed - then we'd have no problems. Just the inconsistency of it made us move to wrapping biopsies again. We're testing both a new processor (maybe our old VIP is part of the problem) and new cassettes - the new McCormick cassettes. Pat Patterson Methodist Dallas Medical Center patpatterson@mhd.com ---------------------------------------------------------------------- Message: 1 Date: Mon, 12 Jun 2006 12:11:30 -0500 From: "Johnson, Teri" Subject: [Histonet] Re: Why (1 GI biopsy not processed, others are beautiful) To: Message-ID: Content-Type: text/plain; charset="us-ascii" Diane, Do you use screen cassettes? If so, do you make sure they are submerged, or do you have them loose in your tissue processor chamber? I don't know if anybody else has noticed this or has become alarmed by it, but the screen cassettes trap solution and usually have at least one trapped air bubble in there. It's theoretically possible one of your biopsies are sitting up in the air bubble and not getting solution. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 ***** *********************************************************************** This electronic transmission contains information from Methodist Health System and should be considered confidential and privileged. The information contained in the above messages is intended only for the use of the individual(s) and entity(ies) named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution, or use of this information is prohibited. If you receive this transmission in error, please notify the sender immediately by return e-mail. Methodist Health System, its subsidiaries and affiliates hereby claim all applicable privileges related to the transmission of this communication. From PMonfils <@t> Lifespan.org Mon Jun 12 13:52:59 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Mon Jun 12 13:53:13 2006 Subject: [Histonet] viability question Message-ID: <09C945920A6B654199F7A58A1D7D1FDE0171773B@lsexch.lsmaster.lifespan.org> A reagent we often use in flow cytometry for viability assessment of cells in suspension is fluorescein diacetate. It quickly permeates cell membranes, and living cells rapidly convert it into fluorescein through the action of cytoplasmic esterases. Fluorescein diacetate is not fluorescent, but fluorescein is. Therefore if the cells become fluorescent in the presence of FD, they are alive. I have not tried this with whole tissues, but it's a quick and easy method to try (assuming of course that you have a fluorescence microscope available). Fluorescence is detectable by flow cytometry within a few seconds of adding the reagent to the cells. It might take a little longer before fluorescence is directly visible to the eye because the cytometer is much more sensitive than the human eye is. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Fawn > Jones > Sent: Monday, June 12, 2006 8:32 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] viability question > > Dear histonetters, > > I am looking for some information for a friend. She wants to find out > if her tissues (aortas) are live or not. Does anyone have any > experience performing viability stains on tissue samples? She tried the > Molecular Probes Live/Dead kit but found it insufficient due to > permeabilty issues. She also suggested MTT, but we're not sure how well > that stain works with tissues. Any help would be greatly appreciated. > > Thank you > Fawn Jones HT(ASCP) > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From bhewlett <@t> cogeco.ca Mon Jun 12 14:10:02 2006 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Mon Jun 12 14:10:09 2006 Subject: [Histonet] ER - fixation References: <448D7C6002000077000006AD@hcnwgwds01.hh.chs> Message-ID: <000f01c68e53$cf289190$6500a8c0@mainbox> Hi Annette, I concur with Richard's recommendation re the Goldstein article. However, I should point out that the suggested minimum 6-8 hours of fixation for ER will only provide approximately 95% confidence for a positive ER result. Low level ER expressers may still be missed, depending upon your IHC system sensitivity. In addition, 6-8 hours NBF fixation is way too short for reliable HER2 staining, and of course you may have to perform IHC for this marker on the same material!! 24 hours fixation in NBF at RT, or 18 hours at 37C should be considered the minimum time for reliable IHC on breast tumours. Bryan R. Hewlett Consultant Technologist Quality Management Program-Laboratory Services. Ontario, Canada. ----- Original Message ----- From: "Richard Cartun" To: ; Sent: Monday, June 12, 2006 2:38 PM Subject: [Histonet] ER - fixation > Hello Annette: > > I could not reply to your original e-mail. > > Regarding ER and fixation, see Goldstein NS, et al.: Minimum formalin > fixation time for consistent ER IHC staining of invasive breast > carcinoma. Am J Clin Pathol 2003;120:86-92. > > Richard > > Richard W. Cartun, Ph.D. > Director, Immunopathology & Histology > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-0174 Fax > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From DOOLEEO <@t> shands.ufl.edu Mon Jun 12 07:35:38 2006 From: DOOLEEO <@t> shands.ufl.edu (Elaine Dooley) Date: Mon Jun 12 14:10:18 2006 Subject: [Histonet] BKV background Message-ID: Dear Histonetters, I think I solved my problem I had with the edge artifact staining with BK virus antibody. (BK virus large T antigen). Instead of using Protease to unmask the antigen I changed to heat induced epitope retrieval with Trilogy (Cell Marque) and I also had to dilute the antibody much further with this retrieval and so far I have not seen the edge artifact again but I am doing some more today and hope they are good as well. Elaine Dooley Shands Teaching Hospital 352-265-0111 ext 72117 From lblazek <@t> digestivespecialists.com Mon Jun 12 14:16:05 2006 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Mon Jun 12 14:13:03 2006 Subject: [Histonet] RE: Histonet - Screened cassettes Message-ID: <6CBA6DC98A079D408C87250591D9DFB80233E05E@bruexchange.digestivespecialists.com> What are the new McCormick cassettes? Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patterson, Pat Sent: Monday, June 12, 2006 2:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] RE: Histonet - Screened cassettes Teri and Diane- We have noticed the same issue you both have mentioned. We stopped using the screened cassettes and now have stopped using the all plastic Micro Cassettes. We have great biopsies for days then 1 or 2 a day would be unprocessed - then we'd have no problems. Just the inconsistency of it made us move to wrapping biopsies again. We're testing both a new processor (maybe our old VIP is part of the problem) and new cassettes - the new McCormick cassettes. Pat Patterson Methodist Dallas Medical Center patpatterson@mhd.com ---------------------------------------------------------------------- Message: 1 Date: Mon, 12 Jun 2006 12:11:30 -0500 From: "Johnson, Teri" Subject: [Histonet] Re: Why (1 GI biopsy not processed, others are beautiful) To: Message-ID: Content-Type: text/plain; charset="us-ascii" Diane, Do you use screen cassettes? If so, do you make sure they are submerged, or do you have them loose in your tissue processor chamber? I don't know if anybody else has noticed this or has become alarmed by it, but the screen cassettes trap solution and usually have at least one trapped air bubble in there. It's theoretically possible one of your biopsies are sitting up in the air bubble and not getting solution. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 ***** *********************************************************************** This electronic transmission contains information from Methodist Health System and should be considered confidential and privileged. The information contained in the above messages is intended only for the use of the individual(s) and entity(ies) named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution, or use of this information is prohibited. If you receive this transmission in error, please notify the sender immediately by return e-mail. Methodist Health System, its subsidiaries and affiliates hereby claim all applicable privileges related to the transmission of this communication. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DOOLEEO <@t> shands.ufl.edu Mon Jun 12 10:18:58 2006 From: DOOLEEO <@t> shands.ufl.edu (Elaine Dooley) Date: Mon Jun 12 14:13:53 2006 Subject: [Histonet] T antigen vendor Message-ID: Dear Histonetters and Vendors, We used to get T antigen (Thomsen-Friedenreich Antigen) from DAKO but they have discontinued it. Does anyone out there know of another vendor for this antibody? Elaine Dooley Shands Teaching Hospital 352-265-0111 ext 7-2117 From brian.chelack <@t> usask.ca Mon Jun 12 13:18:54 2006 From: brian.chelack <@t> usask.ca (Brian Chelack) Date: Mon Jun 12 14:19:18 2006 Subject: [Histonet] CAP question on monitoring lab temp/humidity Message-ID: <001d01c68e4c$aa3cae40$4f13e980@PDS11> Hello Joe; If your goal is to minimize queries from the regulatory people then you might consider using a HOBO data logger which can be set to automatically take temperature and RH. This unit is real nice because you can program the interval time from a few seconds to many days. It will also download the data, graph the data, almost everything except your taxes and make your coffee. They are battery operated and portable. We have used them to monitor freezers, fridges, ambient temps. Mine is pretty ancient (kinda like me), so I'm sure they have come up with newer spiffier models and they aren't too expensive. Right now the CAP guys aren't asking for regulated levels, they are obviously just in the information gathering stage, and if they get an indication that temperature and relative humidity can affect lab results, you can be sure that this will become more of a pain. To be honest we found that relative humidity can affect the performance of our autostainers, but its the real low RH seen here in the winter that creates havoc (typically the lab can be down in the 10-15% RH in January, if you are wearing your silk jockeys, you can zap a spark at your equipment from a half an inch away- hard drives hate this sort of stuff). Back to the topic though, you could set up the equipment to record the data, graph it for yourself and have a look at it when the lab is having one of those weeks... or just show to the Cap guys the "monitored values". Brian Chelack Prairie Diagnostic Services Saskatoon, SK. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Joe Nocito Sent: Monday, June 12, 2006 7:58 AM To: Histonetters Subject: [Histonet] CAP question on humidity levels Okay, I can't stay quiet any longer. The new general checklist question asks if the temperature and humidity levels are monitored. Okay, but they don't state a range or what to do if the temp & humidity are out of range. Has any one come up with an idea? And people wonder why I have a problem with CAP. Happy Monday. Joe Nocito, BS, HT(ASCP) QIHC Histology Manager Pathology Reference Lab San Antonio, TX Brian Chelack Prairie Diagnostic Services 2604-52 Campus Drive Saskatoon SK S7N 5B4 306-966-7241 From cfavara <@t> niaid.nih.gov Mon Jun 12 11:36:22 2006 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID) [E]) Date: Mon Jun 12 14:21:31 2006 Subject: [Histonet] IHC on Ticks? Message-ID: All, Has anyone done this? c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives From langxingpan <@t> pantomics.com Mon Jun 12 14:49:37 2006 From: langxingpan <@t> pantomics.com (Langxing Pan) Date: Mon Jun 12 14:49:48 2006 Subject: [Histonet] Re: Tissue control for CD56 (langxing Pan) Message-ID: <003101c68e59$573c21f0$210110ac@Pantomics> Dear Castillos, We use brain tissue for CD56 IHC control. You can also use melanoma tissue as IHC control for CD56. We have a tissue array product, UNC241 (http://www.pantomics.com/products/UNC241.htm), that can serve as IHC positive control for CD56 as we as controls for >90% of the markers currently used in routine IHC. I can send you some samples to try if you like. Sincerely, Langxing Pan, M.D., Ph.D. Pantomics, Inc. 457 Mariposa Street San Francisco, CA 94107 Direct: 1-415-863-2380 Fax: 1-510-653-1227 langxingpan@pantomics.com www.pantomics.com Message: 4 Date: Mon, 12 Jun 2006 09:12:17 -0400 From: "Castillos, Luminita" Subject: [Histonet] Tissue control for CD56 To: Message-ID: Content-Type: text/plain; charset="us-ascii" Good morning everyone, I am working with human endometrial FFPE tissue and I am doing IHC for NK using CD56 monoclonal antibody. I would like to ask which will be a good positive tissue control and a negative tissue control for CD56?. Also, if there is a vendor from where I can buy these tissue slides?. Thank you so much. Sincerely, Luminita Luminita Castillos, Ph.D. Research Scientist Cogenics(r), A Division of Clinical Data(r) Comprehensive Pharmacogenomics & Molecular Services(tm) 108 Alexader Dr. RTP, NC 27709 Tel: 919-425-2967 www.cogenics.com From hborgeri <@t> wfubmc.edu Mon Jun 12 15:08:43 2006 From: hborgeri <@t> wfubmc.edu (Hermina Borgerink) Date: Mon Jun 12 15:09:09 2006 Subject: [Histonet] Corynebacterium and anthrax bacillus antibodies on FFPE tissue Message-ID: <9AEEF1FB6254224AA355ED285F849165194B10B2@EXCHVS2.medctr.ad.wfubmc.edu> Does anyone know if currently there are antibodies available for the immunohistochemical demonstration on FFPE tissues for corynebacterium and the anthrax bacillus? If so, any information regarding vendor(s) would be greatly appreciated. Hermina Hermina M. Borgerink, BA, HT, HTL(ASCP)QIHC Wake Forest University Health Sciences Department of Pathology Medical Center Blvd. Winston-Salem, NC 27157 Tel. (336) 716-1538 Fax. (336) 716-1515 e-mail hborgeri@wfubmc.edu "Treat a man as he appears to be, and you make him worse. But treat a man as if he already were what he potentially could be, and you make him what he should be." (Goethe) This electronic message, including any attachments, is confidential and proprietary and is solely for the intended recipient. If you are not the intended recipient, this message was sent to you in error and you are hereby advised that any review, disclosure, copying, distribution or use of this message, or any of the information included therein, is unauthorized and strictly prohibited. If you have received this electronic transmission in error, please immediately notify the sender by reply and permanently delete all copies of this message and its attachments. Thank you. From michael.owen <@t> fda.hhs.gov Mon Jun 12 15:16:03 2006 From: michael.owen <@t> fda.hhs.gov (Owen, Michael P) Date: Mon Jun 12 15:16:27 2006 Subject: [Histonet] Corynebacterium and anthrax bacillus antibodies on FFPE tissue Message-ID: Dear Hermina, The CDC Laboratory Response Network (LRN) provides direct fluorescent antibodies (DFA) for detecting Bacillus anthracis to its member laboratories. The antibodies are restricted to the member facilities of the LRN for security reasons. The article below discusses a DFA method for detecting Bacillus anthracis. De BK, Bragg SL, Sanden GN, Wilson KE, Diem LA, Marston CK, et al. A two-component direct fluorescent-antibody assay for rapid identification of Bacillus anthracis. Emerg Infect Dis [serial online] 2002 Oct [date cited];8. Available from: URL: Is this information relevent to your question or are you asking about something very different? Sincerely, Michael Michael P. Owen, Regulatory Microbiologist U.S. FDA Pacific Regional Lab Northwest 22201 23rd Drive SE Bothell, WA 98021-4421 Phone: 425-483-4865 E-Mail: michael.owen@fda.hhs.gov From settembr <@t> umdnj.edu Mon Jun 12 16:06:50 2006 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Mon Jun 12 16:07:33 2006 Subject: [Histonet] HTLV-1 commercially available? Message-ID: Has anyone been using HTLV-1 on formalin fixed paraffin embedded human tissue? I am looking for vendor information and maybe the dilution too. Thanks. Dana Settembre University Hospital - UMDNJ Newark, NJ From mcauliff <@t> umdnj.edu Mon Jun 12 16:10:40 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Mon Jun 12 16:10:09 2006 Subject: [Histonet] Minimizing shrinkage In-Reply-To: References: Message-ID: <448DD850.1010006@umdnj.edu> Hi Tim: Any processing will induce some shrinkage so my first suggestion is to measure fresh or fixed tissue with a dissecting microscope. If that is not possible I suggest a thorough fixation in formalin or formalin+glutaraldehyde followed by embedding in an epoxy resin (Epon substitute). Much less shrinkage in epoxies as compared to wax or methacrylates. Geoff Timothy Macatee wrote: >I have someone who wants to measure cardiac vessel diameter. Does anyone >have an idea of the best process to avoid shrinkage of the tissue? Notice, >how I made no reference to any situation comedy television shows. > >Tim > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From jnocito <@t> satx.rr.com Mon Jun 12 18:22:26 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Jun 12 18:22:33 2006 Subject: [Histonet] CAP question on humidity levels References: Message-ID: <005401c68e77$11f4dbf0$0b69ce44@yourxhtr8hvc4p> I can understand temperature, but humidity? When I was AFIP, we had a terrible time with temperature. It was too cold in the winter (such that we had to wear our coats) and too hot in the summer (such that, we wore T-shirts and shorts. And yes, for those who are thinking this, we did have wet T-shirt contests and I always won.And it just wasn't because I was the boss either). This is the first time that I have seen this question on the General checklist. So what happens if we have a relative humidity of 85%? Okay, so we record it and gone about our business. This is a facility management problem. Again, just busy work. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Jennifer MacDonald" To: "Joe Nocito" Cc: "Histonetters" ; Sent: Monday, June 12, 2006 10:58 AM Subject: Re: [Histonet] CAP question on humidity levels > Blood bank room temperatures are critical for correct testing. This is on > the general checklist, so it applies to the lab as a whole. > > > > > > > "Joe Nocito" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 06/12/2006 06:57 AM > > To > "Histonetters" > cc > > Subject > [Histonet] CAP question on humidity levels > > > > > > > Okay, I can't stay quiet any longer. The new general checklist > question asks if the temperature and humidity levels are monitored. Okay, > but they don't state a range or what to do if the temp & humidity are out > of > range. Has any one come up with an idea? And people wonder why I have a > problem with CAP. > > Happy Monday. > > > > Joe Nocito, BS, HT(ASCP) QIHC > > Histology Manager > > Pathology Reference Lab > > San Antonio, TX > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.394 / Virus Database: 268.8.3/361 - Release Date: 6/11/2006 > > From jnocito <@t> satx.rr.com Mon Jun 12 18:34:12 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Jun 12 18:34:18 2006 Subject: [Histonet] formalin disposal References: <20060612172436.91656.qmail@web30715.mail.mud.yahoo.com> Message-ID: <009401c68e78$b6052cd0$0b69ce44@yourxhtr8hvc4p> we change it once a week and top it off as needed. By Friday, it looks pretty nasty, but we have fun guessing what color it's going to be. Look out, I see a CAP question looming in the background. ANP 1006500 How often is your storage formalin changed and is recorded? sorry, couldn't help it Joe ----- Original Message ----- From: "heidi gordon" To: Sent: Monday, June 12, 2006 12:24 PM Subject: [Histonet] formalin disposal >I work at a facility that processes its own tissue. > We keep the tissue blocks in a bucket of formalin > until it is put on the processor at the end of the > day. I have been disposing of it at the end of each > day. I am wondering how often most facilities dispose > of this formalin. Everyday? When it looks dirty? > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.394 / Virus Database: 268.8.3/361 - Release Date: 6/11/2006 > > From jnocito <@t> satx.rr.com Mon Jun 12 18:39:01 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Jun 12 18:39:09 2006 Subject: [Histonet] DAB disposal References: Message-ID: <00bb01c68e79$644eca30$0b69ce44@yourxhtr8hvc4p> thanks to all to answered my DAB waste question. Some suggestions were to use that DABout filtering system, adding bleach to the container and letting it soak overnight, sending the containers to CAP in Chicago. Oops, there I go again. I'm sorry. I'll try to behave. Yeah, right. Joe ----- Original Message ----- From: "Anne Van Binsbergen" To: "Tom McNemar" ; "Joe Nocito" ; Sent: Monday, June 12, 2006 8:24 AM Subject: RE: [Histonet] DAB disposal Hi Tom Today we became proud owners of a brand new Dako unit and I had that exact thought in mind - what to do with a 20 litre bottle of dab-contaminated waste....every couple of weeks - here in the UAE we have little choice as there are no federal laws and no off-site waste companies to cart waste off even for a fee. I am stuck until someone in authority writes the book of rules. I just know that one of you will go 'gasp!shock!horror'! and insist that I be pro-active - I am - some of you know me to be 'tenacious' - this is correct - but even that has not helped me here. Advice has been offered, best practice quoted, OH&S quoted, internet sites accessed, printed, handed over in report form, promises are made by those in authority and then broken. We stockpile safely off site and wait This is true for ALL toxic waste in this laboratory, as well as the rest of the labs, together with mercury filled blood pressure cuffs, some broken, 'expired' rat poison, old mercury thermometers, 'unknown' unlabelled chemicals from shut down labs...some scary stuff But - I digress - back to the DAB....and how to manage the growing volume of dab waste..... Any suggestions.....??? Anyone.....??? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Monday, June 12, 2006 4:59 PM To: Joe Nocito; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] DAB disposal I have the Dako unit. It separates the hazardous/non-hazardous waste. The DAB goes into a 20L carboy that is then hauled off-site for disposal. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Joe Nocito Sent: Friday, June 09, 2006 7:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DAB disposal with the risk of sounding stupid (which happens to me often) how are people disposing their DAB contaminated Ventana waste? I mean, there is so little actual DAB present. I refuse to answer any questions that might incriminate me. I know what you are thinking. I have a new safety office who just happens to be certified in hazmat (yes, for those who know me, it's Hector). Hector keeps bugging me about it. For those who don't know us, Hector and I are joined at the hip. Last year in Ft. Lauderdale, everyone knew something was wrong because they saw Hector without me. No, we are not homosexuals, not that it matters. Hector was my histology instructor and we are really, really close friends. Oh d _ _ m, I'm sure I just offended somebody. I'm leaving now before I say something else wrong. It must be Friday. Why do I do this to myself? Have a safe weekend y'all. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.8.3/361 - Release Date: 6/11/2006 From jnocito <@t> satx.rr.com Mon Jun 12 18:45:53 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Mon Jun 12 18:46:03 2006 Subject: [Histonet] why References: <4C96AA62BADFD81180CB0002A5AD67FB07113A3E@MAILPMA> Message-ID: <00f401c68e7a$57d48e60$0b69ce44@yourxhtr8hvc4p> Diane, do you the screened cassettes? I ran about 25 GI blocks this morning that sat fixing since Saturday and I had to reprocess 2 of them. This happens every so often and it still puzzles me. Sorry I don't have an answer, but you're not alone with this. There I said it, ( in public too) I don't have all the answers. Joe ----- Original Message ----- From: "Santana, Diane" To: Sent: Monday, June 12, 2006 11:40 AM Subject: [Histonet] why > Hi > I am looking for some ideas to help with a problem I am still having with > processing my GI bx's. What would cause my tissue to be raw? Not all, not > some, only 1 or 2 pieces. > Example if I have a case with 4 bx's, maybe only 1 is raw, the other 3 are > beautiful. All my solutions test at the right % and temperatures are fine. > Question again, why does 1 piece of tissue come out raw, when the other > pieces are fine? > thanks in advance, > Diane Santana > PMA > Haverhill, Mass. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.394 / Virus Database: 268.8.3/361 - Release Date: 6/11/2006 > > From ken.turner <@t> stonebow.otago.ac.nz Mon Jun 12 19:09:13 2006 From: ken.turner <@t> stonebow.otago.ac.nz (Ken Turner) Date: Mon Jun 12 19:09:20 2006 Subject: [Histonet] Anti-Fas Ligand antibody Message-ID: <7.0.1.0.0.20060613115337.01d91540@stonebow.otago.ac.nz> Hi everyone, Does anyone have any experience with Anti-Fas Ligand (IgG polyclonal rabbit) antibody and can recommend a HIER method to use on paraffin sections? I have a protocol to test but it merely suggests HIER will be necessary and gives no indication how severe this should be. Any help would be appreciated before I start 'playing' with the protocol. Cheers, Ken. Kenneth W Turner NZCS, BA(Hons), Dip.Teach. Senior Teaching Fellow Director Histology Service Unit Department of Pathology Dunedin School of Medicine e-mail ken.turner@stonebow.otago.ac.nz University of Otago Phone: (03) 479 7135 or (03) 479-7152 New Zealand Fax: (03) 479-7136 From micro <@t> superlink.net Mon Jun 12 19:14:06 2006 From: micro <@t> superlink.net (Markus F. Meyenhofer) Date: Mon Jun 12 19:14:17 2006 Subject: Fw: [Histonet] why Message-ID: <0aa401c68e7e$497bee40$50893cd1@DJ4VDH31> ----- Original Message ----- From: "Markus F. Meyenhofer" To: "Santana, Diane" Sent: Monday, June 12, 2006 8:03 PM Subject: Re: [Histonet] why > Air in your izzy bizzy fine mesh capsules!(fine mesh capsules are CRAP! > Air can not get out and exchange is minimal!) Use old fashion teabag-type > paper and wrap them ONCE, then use a regular cassette (one can put 4-6 > wrapped "packages" across into one capsule. > Regards, > Markus F. Meyenhofer > Microscopy Labs > ----- Original Message ----- > From: "Santana, Diane" > To: > Sent: Monday, June 12, 2006 12:40 PM > Subject: [Histonet] why > > >> Hi >> I am looking for some ideas to help with a problem I am still having with >> processing my GI bx's. What would cause my tissue to be raw? Not all, not >> some, only 1 or 2 pieces. >> Example if I have a case with 4 bx's, maybe only 1 is raw, the other 3 >> are >> beautiful. All my solutions test at the right % and temperatures are >> fine. >> Question again, why does 1 piece of tissue come out raw, when the other >> pieces are fine? >> thanks in advance, >> Diane Santana >> PMA >> Haverhill, Mass. >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > From tahseen <@t> brain.net.pk Mon Jun 12 20:18:24 2006 From: tahseen <@t> brain.net.pk (Muhammad Tahseen) Date: Mon Jun 12 20:20:48 2006 Subject: [Histonet] Computer software for record Gross description and Gross Images Message-ID: <00b301c68e87$462cad20$2fa2fea9@p> Hi every body Is there any one who can recommend me any Computer software to record gross description and gross images for transcription's. Our work lode is 100 specimen a day. Muhammad Tahseen, Histology Supervisor SKMCH&RC Lahore,Pakistan. tahseen@brain.net.pk From jzeliadt <@t> msn.com Mon Jun 12 23:04:40 2006 From: jzeliadt <@t> msn.com (JEFF TATUM ZELIADT) Date: Mon Jun 12 23:04:47 2006 Subject: [Histonet] remove my email address from your list Message-ID: From kutaisi13 <@t> hotmail.com Tue Jun 13 00:20:08 2006 From: kutaisi13 <@t> hotmail.com (david moses) Date: Tue Jun 13 00:20:15 2006 Subject: [Histonet] remove my email address from your list Message-ID: _________________________________________________________________ FREE pop-up blocking with the new MSN Toolbar [1]MSN Toolbar Get it now! References 1. http://g.msn.com/8HMBEN/2755??PS=47575 From louise.renton <@t> gmail.com Tue Jun 13 01:50:54 2006 From: louise.renton <@t> gmail.com (louise renton) Date: Tue Jun 13 01:58:07 2006 Subject: [Histonet] eosin storage Message-ID: Hi all, what is the best way to keep eosinY/phloxine mixture long term (aqueous sloution used for h&E)? I use the solution sporadically, and hate it when i get to the bottle and there is an alien life form evolving in it. Thymol seems no deterrrent. Could I aliquot it and freeze it? Autoclave and store in fridge? any thoughts? Best regards Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From Joseph.Garvin <@t> NUIGALWAY.IE Tue Jun 13 03:28:27 2006 From: Joseph.Garvin <@t> NUIGALWAY.IE (0104684s) Date: Tue Jun 13 03:32:19 2006 Subject: [Histonet] remove my email address from your list Message-ID: <448DA1CD@bodkin.nuigalway.ie> Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jacqueline.Malam <@t> rli.mbht.nhs.uk Tue Jun 13 03:45:08 2006 From: Jacqueline.Malam <@t> rli.mbht.nhs.uk (Malam Jacqueline) Date: Tue Jun 13 03:46:53 2006 Subject: [Histonet] CD56 pos control Message-ID: We use appendix which demonstrates the neural tissues there very well. Jacqui DISCLAIMER: This e-mail is confidential and privileged. If you are not the intended recipient please accept our apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform postmaster@rli.mbht.nhs.uk that this message has gone astray before deleting it. Comments or opinions expressed in this email are those of their respective contributors only. The views expressed do not represent the views of the Trust, its management or employees. University Hospitals of Morecambe Bay NHS Trust is not responsible and disclaims any and all liability for the content of comments written within.Thank you for your co-operation. From EJones <@t> Ventanamed.com Tue Jun 13 03:57:19 2006 From: EJones <@t> Ventanamed.com (Emma JONES) Date: Tue Jun 13 06:31:39 2006 Subject: [Histonet] RE: DAB disposal Message-ID: Hi, I agree, there are obviously limitations as to what should be thrown = down the sink, but not all labs have methods of disposal such as = companies to remove and dispose of. Or the money to invest in dab = removal systems. I have worked as a BMS in a number of labs, and I certainly do not know = of many labs who can capture and contain all their waste formalin whilst = doing grossing/cut up, contain the waste whilst performing special = stains, what happens to the xylene, alcohol, silver, and even the dab, = peroxide etc waste gathered whilst doing immuno manually? How about some = of the bleaches and detergents used to clean labs, and the disinfectants = used as infection control? The list goes on.......=20 The method I mentioned as far as I know has been used by a number of = labs for a long time, not just specifically Ventana. Joe you mentioned = you had used it, but perhaps in larger quantities. In a perfect, ideal, environmentally friendly world, we would not have = solvents etc, but we don't, and finding companies to remove lab waste = who do not charge high premiums that NHS labs, can afford is difficult. Certainly a discussion that needs to be addressed with regulations and = specific guidelines put firmly in place. Regards Emma F-----Original Message-----rom: = histonet-bounces@lists.utsouthwestern.edu = [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of = histonet-request@lists.utsouthwestern.edu Sent: Monday, June 12, 2006 7:23 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 31, Issue 18[Scanned] --=20 No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.8.3/359 - Release Date: = 08/06/2006 =20 ------------------------------ Message: 3 Date: Mon, 12 Jun 2006 10:24:36 -0700 (PDT) From: heidi gordon Subject: [Histonet] formalin disposal To: histonet@lists.utsouthwestern.edu Message-ID: <20060612172436.91656.qmail@web30715.mail.mud.yahoo.com> Content-Type: text/plain; charset=3Diso-8859-1 I work at a facility that processes its own tissue.=20 We keep the tissue blocks in a bucket of formalin until it is put on the processor at the end of the day. I have been disposing of it at the end of each day. I am wondering how often most facilities dispose of this formalin. Everyday? When it looks dirty? =20 __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around=20 http://mail.yahoo.com=20 Emma, Is this what Ventana advises? Labs should check with their local waste=20 water treatment plant before TREATING anything or putting "diluted" DAB=20 down the drain. If laboratories are generating this kind of waste on a=20 weekly basis, this adds up over time. In general, waste water plants do = not=20 like this and EPA does monitor water around here. Montana has discharge = rules (maybe for larger industries) but our city does not want = medicines,=20 household cleaners, solvents, fertilizers, pesticides, oil, etc, flushed = down the drains and warn residents frequently about this in their water = bills. Wouldn't it be better to collect and have it hauled away for proper=20 chemical disposal than add even "minute" amounts of a potential = carcinogen=20 to our water supply. We use very little DAB in our lab, but all=20 chromogens AEC, DAB, permanent red, etc, (very low volume usage) are=20 collected for chemical waste pickup and proper disposal. An interesting sidelight, tested water wells in parts of Montana now = have=20 traces of sunscreen chemical, medicines, herbicides, nitrates and=20 nitrites. So much for the "pristine" environment and pure water out in = the Wild Wild West /Rocky Mountain region. Drink beer and brush you = teeth=20 with it too, it may be safer when you visit some Montana dude ranch! Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 Message: 1 Date: Fri, 9 Jun 2006 12:29:00 -0500 From: jhaviland@mdanderson.org Subject: [Histonet] Vectastain elite kits To: histonet@lists.utsouthwestern.edu Message-ID =20 Dear Histonetters: I have the Dako unit. It separates the hazardous/non-hazardous waste. = The DAB goes into a 20L carboy that is then hauled off-site for = disposal. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org Hi Tom Today we became proud owners of a brand new Dako unit and I had that exact thought in mind - what to do with a 20 litre bottle of dab-contaminated waste....every couple of weeks - here in the UAE we have little choice as there are no federal laws and no off-site waste companies to cart waste off even for a fee.=20 I am stuck until someone in authority writes the book of rules.=20 I just know that one of you will go 'gasp!shock!horror'! and insist that I be pro-active - I am - some of you know me to be 'tenacious' - this is correct - but even that has not helped me here. Advice has been offered, best practice quoted, OH&S quoted, internet sites accessed, printed, handed over in report form, promises are made by those in authority and then broken. We stockpile safely off site and wait=20 This is true for ALL toxic waste in this laboratory, as well as the rest of the labs, together with mercury filled blood pressure cuffs, some broken, 'expired' rat poison, old mercury thermometers, 'unknown' unlabelled chemicals from shut down labs...some scary stuff But - I digress - back to the DAB....and how to manage the growing volume of dab waste..... Any suggestions.....??? Anyone.....??? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom McNemar Sent: Monday, June 12, 2006 4:59 PM To: Joe Nocito; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] DAB disposal I have the Dako unit. It separates the hazardous/non-hazardous waste. The DAB goes into a 20L carboy that is then hauled off-site for disposal. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org --=20 No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.8.3/359 - Release Date: = 08/06/2006 =20 From cornettl <@t> hotmail.com Tue Jun 13 07:24:19 2006 From: cornettl <@t> hotmail.com (Lorraine Cornett) Date: Tue Jun 13 07:24:26 2006 Subject: [Histonet] why In-Reply-To: <00f401c68e7a$57d48e60$0b69ce44@yourxhtr8hvc4p> Message-ID: We had this happen on occassion as well. We were using biopsy cassettes and determined that they (because of the small holes to keep tissue in) were allowing air bubbles to be in the cassette. If you had 1 piece out of 4 that happened to be in the air bubble, it wouldn't process properly. Don't know if this is the case in your lab, but we determined that to be our problem. Keep us all posted...another mystery of histology! Lorraine Cornett, HT (ASCP) Highlands Pathology Blue Ridge Division, Kingsport, TN 423 224-5793 fax 423 224-5349 >From: "Joe Nocito" >To: "Santana, Diane" >, >Subject: Re: [Histonet] why >Date: Mon, 12 Jun 2006 18:45:53 -0500 > >Diane, >do you the screened cassettes? I ran about 25 GI blocks this morning that >sat fixing since Saturday and I had to reprocess 2 of them. This happens >every so often and it still puzzles me. > Sorry I don't have an answer, but you're not alone with this. > There I said it, ( in public too) I don't have all the answers. > >Joe >----- Original Message ----- From: "Santana, Diane" > >To: >Sent: Monday, June 12, 2006 11:40 AM >Subject: [Histonet] why > > >>Hi >>I am looking for some ideas to help with a problem I am still having with >>processing my GI bx's. What would cause my tissue to be raw? Not all, not >>some, only 1 or 2 pieces. >>Example if I have a case with 4 bx's, maybe only 1 is raw, the other 3 are >>beautiful. All my solutions test at the right % and temperatures are fine. >>Question again, why does 1 piece of tissue come out raw, when the other >>pieces are fine? >>thanks in advance, >>Diane Santana >>PMA >>Haverhill, Mass. >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >>-- >>No virus found in this incoming message. >>Checked by AVG Free Edition. >>Version: 7.1.394 / Virus Database: 268.8.3/361 - Release Date: 6/11/2006 >> >> > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ FREE pop-up blocking with the new MSN Toolbar – get it now! http://toolbar.msn.click-url.com/go/onm00200415ave/direct/01/ From ROrr <@t> enh.org Tue Jun 13 07:43:54 2006 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Tue Jun 13 07:44:00 2006 Subject: [Histonet] Dab disposal Message-ID: Gayle brings up interesting points in what has been found in the well waters. As far as vendors supplying DAB disposal recommendations, they really can't commit, other than to have you check it out locally. Every local water plant has different procedures. I would check with my hospital's Haz Mat person and if that person isn't knowledgeable, give a call to the water treatment plant. I have been looking into the DAB OUT system from TBS, to at least separate the solid from the liquid. And then dispose of the filtered (solid?) waste Any opinions on this set up? Becky Orr CLA,HT(ASCP) Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 -----Original Message----- From stern <@t> ipmc.cnrs.fr Tue Jun 6 10:07:20 2006 From: stern <@t> ipmc.cnrs.fr (Alejandro Ortiz Stern) Date: Tue Jun 13 09:22:43 2006 Subject: [Histonet] Help with Lung architecture Message-ID: <44859A28.2070300@ipmc.cnrs.fr> Hi, I am currently searching for a protocol to maintain Lung structure intact while avoiding intra tracheal instillation of liquids since I am very interested in cells that lie in the outer-most layers of the lung (cells that would normally become detached upon lung lavage) Or if such cells remain attached during paraformaldehyde instillation or OCT instillation that would also be very valuable information (I have no idea). If anyone has any suggestions, or knows how to fix/cut air inflated lungs (for example if the trachea is closed before the opening of the thoracic cavity or so) I would be extremely grateful for the advise. The idea is to use these sections for confocal microscopy. Thank You All in advance. -- Alejandro Ortiz Stern M.D. Ph.D., Postdoctoral Fellow Institut National de la Sant? et de la Recherche M?dicale, E0344 Universit? de Nice-Sophia-Antipolis Institut de Pharmacologie Mol?culaire et Cellulaire 660 Route des Lucioles - 06560 Valbonne - FRANCE TEL: 33 (4) 93 95 77 80 - FAX: 33 (4) 93 95 77 08 From PMonfils <@t> Lifespan.org Tue Jun 13 09:24:06 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Tue Jun 13 09:24:16 2006 Subject: [Histonet] eosin storage Message-ID: <09C945920A6B654199F7A58A1D7D1FDE0171773C@lsexch.lsmaster.lifespan.org> Why not use an alcoholic solution instead of aqueous? I make up a 5X eosin/phloxine stock solution in 95% ethanol, then keep it on the shelf in a brown bottle. It keeps forever and nothing can grow in it. For use I dilute it 1 part stock to 4 parts 80% ethanol. Switching to alcoholic solutions has helped in some other cases too. Many years ago we did a Grocott fungus stain (GMS) and when we checked the slide microscopically we saw that there were a lot of fungal hyphae present. But then we noticed that the fungi were not silver stained. And that they were not in the same plane of focus as the tissue section. And that in some areas they extended out past the edges of the tissue section. The fungus was growing in the aqueous light green solution used as a counterstain. I switched to alcoholic light green (or fast green) solutions and have never had a problem since. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > louise renton > Sent: Monday, June 12, 2006 11:50 PM > To: Histonet@lists.utsouthwestern.edu > Subject: [Histonet] eosin storage > > Hi all, > > what is the best way to keep eosinY/phloxine mixture long term > (aqueous sloution used for h&E)? > I use the solution sporadically, and hate it when i get to the bottle > and there is an alien life form evolving in it. Thymol seems no > deterrrent. Could I aliquot it and freeze it? Autoclave and store in > fridge? any thoughts? > > Best regards > Louise Renton > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > "There are nights when the wolves are silent and only the moon howls". > George Carlin > No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From timo.vaisanen <@t> oulu.fi Tue Jun 13 09:33:58 2006 From: timo.vaisanen <@t> oulu.fi (Timo =?iso-8859-1?b?VuRpc+RuZW4=?=) Date: Tue Jun 13 09:34:05 2006 Subject: [Histonet] Trichrome staining and fibrin Message-ID: <1150209238.448eccd6cd4c0@webmail.oulu.fi> Hello all, I have struggled with getting Masson Trichrome (Goldner) staining to work with kidney biopsies. The problem is that our pathologist does not get positive fibrin staining to glomeruli in diseased kidneys. Those areas of the glomeruli that should contain fibrin, at least according to the pathologist, are green (Lichtgrun) and not red, as they should be, whatever I do. I have tried to enhance the staining with Bouin's fixative (+56C) pretreatment but it did not change the overall situation. However, the red stain was more intense in skin samples containing fibrin (formalin fixed). I have also tried another Trichrome staining with Crocein Scarlet 7B without any luck. In our lab kidney biopsies are routinely fixed with alcoholic Bouin's. As far as I know it should be compatible with the Masson protocol we use. Any ideas? Any good protocols that I could try? Thank's in advance, Timo From bhewlett <@t> cogeco.ca Tue Jun 13 09:52:05 2006 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Tue Jun 13 09:52:14 2006 Subject: [Histonet] Trichrome staining and fibrin References: <1150209238.448eccd6cd4c0@webmail.oulu.fi> Message-ID: <000701c68ef8$f0fa58d0$6500a8c0@mainbox> Timo, Try the Lendrum MSB trichrome variant, it was designed to demonstrate fibrin. I will send the method via a separate e-mail. Bryan ----- Original Message ----- From: "Timo V?is?nen" To: Sent: Tuesday, June 13, 2006 10:33 AM Subject: [Histonet] Trichrome staining and fibrin > Hello all, > > I have struggled with getting Masson Trichrome (Goldner) staining to work > with > kidney biopsies. The problem is that our pathologist does not get positive > fibrin staining to glomeruli in diseased kidneys. Those areas of the > glomeruli > that should contain fibrin, at least according to the pathologist, are > green > (Lichtgrun) and not red, as they should be, whatever I do. I have tried to > enhance the staining with Bouin's fixative (+56C) pretreatment but it did > not > change the overall situation. However, the red stain was more intense in > skin > samples containing fibrin (formalin fixed). I have also tried another > Trichrome > staining with Crocein Scarlet 7B without any luck. In our lab kidney > biopsies > are routinely fixed with alcoholic Bouin's. As far as I know it should be > compatible with the Masson protocol we use. Any ideas? Any good protocols > that > I could try? > > Thank's in advance, > > Timo > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Terry.Marshall <@t> rothgen.nhs.uk Tue Jun 13 10:27:56 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Tue Jun 13 10:25:03 2006 Subject: [Histonet] Trichrome staining and fibrin Message-ID: Bryan, You know full well that MSB required initial staining in mercury. I know that zinc is an adequate replacement. Attempting to get a consistent good red colour with formalin fixed material is futile, as is post-fixation. At times it works and at times not. IMO, Masson is useless for fibrin. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Bryan Hewlett [mailto:bhewlett@cogeco.ca] Sent: 13 June 2006 15:52 To: Timo V?is?nen; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Trichrome staining and fibrin Timo, Try the Lendrum MSB trichrome variant, it was designed to demonstrate fibrin. I will send the method via a separate e-mail. Bryan ----- Original Message ----- From: "Timo V?is?nen" To: Sent: Tuesday, June 13, 2006 10:33 AM Subject: [Histonet] Trichrome staining and fibrin > Hello all, > > I have struggled with getting Masson Trichrome (Goldner) staining to work > with > kidney biopsies. The problem is that our pathologist does not get positive > fibrin staining to glomeruli in diseased kidneys. Those areas of the > glomeruli > that should contain fibrin, at least according to the pathologist, are > green > (Lichtgrun) and not red, as they should be, whatever I do. I have tried to > enhance the staining with Bouin's fixative (+56C) pretreatment but it did > not > change the overall situation. However, the red stain was more intense in > skin > samples containing fibrin (formalin fixed). I have also tried another > Trichrome > staining with Crocein Scarlet 7B without any luck. In our lab kidney > biopsies > are routinely fixed with alcoholic Bouin's. As far as I know it should be > compatible with the Masson protocol we use. Any ideas? Any good protocols > that > I could try? > > Thank's in advance, > > Timo > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Heather.A.Harper <@t> pcola.med.navy.mil Tue Jun 13 10:21:38 2006 From: Heather.A.Harper <@t> pcola.med.navy.mil (Heather.A.Harper@pcola.med.navy.mil) Date: Tue Jun 13 10:27:00 2006 Subject: [Histonet] High Profile Micrtome blades Message-ID: I have a Leica Microtome 2235. Currently I am using the Leica 818 high profile blades, and they are terrible. They become dull immediately, slowing down my cutting, because it seems I am changing the blade for every block I cut. Does anyone know of a good high profile blade? I was told the high profile blade by DuraEdge is good. Any feedback would be appreciated. Heather A. Harper From rjbuesa <@t> yahoo.com Tue Jun 13 10:39:01 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jun 13 10:39:08 2006 Subject: [Histonet] High Profile Micrtome blades In-Reply-To: Message-ID: <20060613153901.95545.qmail@web61216.mail.yahoo.com> Heather: For me the best disposable blades (both low and high profile) are the "AccuEdge" by Sakura/Feather. Ren? J. Heather.A.Harper@pcola.med.navy.mil wrote: I have a Leica Microtome 2235. Currently I am using the Leica 818 high profile blades, and they are terrible. They become dull immediately, slowing down my cutting, because it seems I am changing the blade for every block I cut. Does anyone know of a good high profile blade? I was told the high profile blade by DuraEdge is good. Any feedback would be appreciated. Heather A. Harper _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From gcallis <@t> montana.edu Tue Jun 13 10:40:21 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Jun 13 10:40:40 2006 Subject: [Histonet] Help with Lung architecture In-Reply-To: <44859A28.2070300@ipmc.cnrs.fr> References: <44859A28.2070300@ipmc.cnrs.fr> Message-ID: <6.0.0.22.1.20060613093635.01b93980@gemini.msu.montana.edu> The only way we have been able to do this is using Instrumedics Cryojane tape transfer device. It is available in Europe, or go to their website to access information. This is the only way we could get 5 um thin sections of air filled lungs. The instrument is NOT cheap, but is certainly the only one we have found that will do this sectioning. II know people in United Kingdom have been able to purchase the instrument. OR use vibrating microtome sections, these will NOT be thin sections, more in the range of 50 um or more but suitable for CLSM and z stack imaging. At 09:07 AM 6/6/2006, you wrote: >Hi, > >I am currently searching for a protocol to maintain Lung structure >intact while avoiding intra tracheal instillation of liquids since I am >very interested in cells that lie in the outer-most layers of the lung >(cells that would normally become detached upon lung lavage) Or if such >cells remain attached during paraformaldehyde instillation or OCT >instillation that would also be very valuable information (I have no idea). > >If anyone has any suggestions, or knows how to fix/cut air inflated >lungs (for example if the trachea is closed before the opening of the >thoracic cavity or so) I would be extremely grateful for the advise. > >The idea is to use these sections for confocal microscopy. > >Thank You All in advance. > >-- >Alejandro Ortiz Stern M.D. Ph.D., >Postdoctoral Fellow >Institut National de la Sant? et de la Recherche M?dicale, E0344 >Universit? de Nice-Sophia-Antipolis >Institut de Pharmacologie Mol?culaire et Cellulaire >660 Route des Lucioles - 06560 Valbonne - FRANCE >TEL: 33 (4) 93 95 77 80 - FAX: 33 (4) 93 95 77 08 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From Diane.Gladney <@t> se.amedd.army.mil Tue Jun 13 10:42:50 2006 From: Diane.Gladney <@t> se.amedd.army.mil (Gladney, Diane C Ms MACH) Date: Tue Jun 13 10:44:20 2006 Subject: [Histonet] High Profile Micrtome blades In-Reply-To: Message-ID: <4D55B2E997EFAE4DA6081DDE100B8302E9D123@amedmlsermc133> C.L. Sturkey makes excellent high and low profile blades. I have been using them for years. Thanks, Diane Diane C. Gladney, HT (ASCP) Supervisor, Histology Dept. Moncrief Army Community Hospital Dept. of Pathology 4500 Stuart St. FT. Jackson, SC 29207 Email: diane.gladney@se.amedd.army.mil Phone: 803-751-2530 FAX: 803-751-7829 DSN: 734-2530 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heather.A.Harper@pcola.med.navy.mil Sent: Tuesday, June 13, 2006 11:22 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] High Profile Micrtome blades I have a Leica Microtome 2235. Currently I am using the Leica 818 high profile blades, and they are terrible. They become dull immediately, slowing down my cutting, because it seems I am changing the blade for every block I cut. Does anyone know of a good high profile blade? I was told the high profile blade by DuraEdge is good. Any feedback would be appreciated. Heather A. Harper _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Margaret.Perry <@t> sdstate.edu Tue Jun 13 10:48:41 2006 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Tue Jun 13 10:48:57 2006 Subject: [Histonet] substrates Message-ID: We are trying to do double staining and are looking for two different colors of substrate and we can't use DAB due to melanin. Does anyone know of a green stain that is xylene compatible? Vector has Nova Red and a blue color but we use hematoxylin as a counterstain. It would mean an extra step for us to counterstain in Methyl Green We use a DAKO autostainer. Margaret Perry HT(ASCP) South Dakota State University Animal Research and Diagnostic Lab Brookings SD 57007 Margaret.Perry@sdstate.edu From histosci <@t> shentel.net Tue Jun 13 10:52:09 2006 From: histosci <@t> shentel.net (HSRL) Date: Tue Jun 13 10:52:24 2006 Subject: [Histonet] High Profile Micrtome blades In-Reply-To: Message-ID: <00bb01c68f01$56a60d20$0500a8c0@HSRLMAIN> Heather, We bought about 30 packs of AccuEdge High profile blades back when we used high profile blades. If you are looking for a bargain on high profile high end blades, we would be willing to sell you the rest of our AccuEdge for about 1/2 the price we paid just to get rid of them. We paid nearly $100 per pack of 50, we would be willing to sell them to you for $50 per pack. If that is appealing to you, let me know. I can send you a left over pack to see how you like them. Let me know what you think. Have a good day, Beth Beth Poole HSRL, Inc.- A GLP Compliant Contract Laboratory 5930 Main Street Mount Jackson, Virginia 22842 540.477.4440 Fax:540.477.4448 beth@hsrl.org www.hsrl.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heather.A.Harper@pcola.med.navy.mil Sent: Tuesday, June 13, 2006 10:22 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] High Profile Micrtome blades I have a Leica Microtome 2235. Currently I am using the Leica 818 high profile blades, and they are terrible. They become dull immediately, slowing down my cutting, because it seems I am changing the blade for every block I cut. Does anyone know of a good high profile blade? I was told the high profile blade by DuraEdge is good. Any feedback would be appreciated. Heather A. Harper _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Margaret.Perry <@t> sdstate.edu Tue Jun 13 11:03:54 2006 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Tue Jun 13 11:04:01 2006 Subject: [Histonet] Coxiella burnetti Message-ID: Integrated Diagnostics has merged and is now PanBio. According to technical support the old antibody catalog number JW261 is no longer available. I have used several antibody search engines but have not been able to come up with another source. Does anyone know where I could get Coxiella burnetti and if it cross reacts with other things? Margaret Perry HT (ASCP) South Dakota State University Animal and Diagnostic Research Laboratory Brookings SD 57007 Margaret.Perry@sdstate.edu From bhewlett <@t> cogeco.ca Tue Jun 13 11:13:57 2006 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Tue Jun 13 11:14:03 2006 Subject: [Histonet] Trichrome staining and fibrin References: Message-ID: <001801c68f04$60390ab0$6500a8c0@mainbox> Terry, Actually NOT! True, Lendrum's original papers(1962) recommended fixation in picro-mercuric alcohol for 3 weeks (a tad unnecessary that!). However, both Prof. Lendrum and Bill Slidders also successfully used formaldehyde fixed material that was treated in Bouin immediately prior to staining. The key to all of the Masson Type trichromes on formaldehyde-fixed tissue, is to use a pretreatment in picric acid. This re-aligns the reactive side chains on the proteins, so that there is a predominance of basic amino groups and hence maximal binding of anionic dyes. Mercury fixation is simply not necessary! Nor is Zinc substitution. (see Troubleshooting Histology stains: Horobin and Bancroft 1998, page 129) The lack of consistency, in obtaining good red colour, is due variability in the staining procedure (the post dye water rinses), NOT the fixation. Once this is addressed, the inconsistency goes away. That is true for the original Masson, Picro-Mallory (all variants), MSB and Masson 44/41. Achieving consistent good red colour with formaldehyde fixed material is easy, if the staining mechanisms are understood and those unnecessary water rinses removed. Been doing it for over 40 years! Bryan (Engineers can prove that the bumble bee simply cannot fly. However, the bumble bee doesn't know that and flies anyway) ----- Original Message ----- From: "Marshall Terry Dr, Consultant Histopathologist" To: "Bryan Hewlett" ; "Timo V?is?nen" ; Sent: Tuesday, June 13, 2006 11:27 AM Subject: RE: [Histonet] Trichrome staining and fibrin Bryan, You know full well that MSB required initial staining in mercury. I know that zinc is an adequate replacement. Attempting to get a consistent good red colour with formalin fixed material is futile, as is post-fixation. At times it works and at times not. IMO, Masson is useless for fibrin. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Bryan Hewlett [mailto:bhewlett@cogeco.ca] Sent: 13 June 2006 15:52 To: Timo V?is?nen; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Trichrome staining and fibrin Timo, Try the Lendrum MSB trichrome variant, it was designed to demonstrate fibrin. I will send the method via a separate e-mail. Bryan ----- Original Message ----- From: "Timo V?is?nen" To: Sent: Tuesday, June 13, 2006 10:33 AM Subject: [Histonet] Trichrome staining and fibrin > Hello all, > > I have struggled with getting Masson Trichrome (Goldner) staining to work > with > kidney biopsies. The problem is that our pathologist does not get positive > fibrin staining to glomeruli in diseased kidneys. Those areas of the > glomeruli > that should contain fibrin, at least according to the pathologist, are > green > (Lichtgrun) and not red, as they should be, whatever I do. I have tried to > enhance the staining with Bouin's fixative (+56C) pretreatment but it did > not > change the overall situation. However, the red stain was more intense in > skin > samples containing fibrin (formalin fixed). I have also tried another > Trichrome > staining with Crocein Scarlet 7B without any luck. In our lab kidney > biopsies > are routinely fixed with alcoholic Bouin's. As far as I know it should be > compatible with the Masson protocol we use. Any ideas? Any good protocols > that > I could try? > > Thank's in advance, > > Timo > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Tue Jun 13 11:15:06 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Jun 13 11:15:18 2006 Subject: More on Re: [Histonet] RE: DAB disposal In-Reply-To: References: Message-ID: <6.0.0.22.1.20060613083149.01b52008@gemini.msu.montana.edu> There has already been a great deal of discussion about this problem, and specific guidelines are often in place and implemented, even in remote areas and for small(or large) laboratories. Have you done a Histonet Archive search on messages concerning this issue, it is there and been going on for a LONG time - well over 10 years or more - this is not a new issue. Our laboratory has been well aware of DAB treatment for disposal and did it for a time, but opted NOT to do it long before we were required by regulation to cease but collect. This treatment never addressed the by product issue i.e. what happens to the DAB after chemical treatment. Are the byproducts going to be just as bad or worse? If a laboratory can afford any kind of expensive immunostaining instrument such you sell, or even if they do manual IHC or other routine stains, then they should be able to afford collection/disposal services or seek such a service. We are a very small user of these compounds you mention i.e DAB, silver, any heavy metal compounds (chromic acid) even if only 25 mls of the stuff - it is collected for disposal. Solvents never go down the drain. As for bleaches, detergents, and disinfectants, wastewater plants do allow certain things but these are also common to households, not just laboratories. As far as I am concerned and there are a lot of others who might agree, ALL labs, no matter if they are the "little guy" need to address and implement chemical waste collection and disposal. The only time this could change is an exemption in a locale but laboratories need to find out if an exemption is in place before the dumping. Once again, users beware - check out what is allowed for your area. I'm curious - you never said if this is your company's stand for DAB disposal or if it is just your comment. Does Ventana advise/tell people to treat their DAB wastes for drain dumping? It certainly would not be a good policy to do so and does not encourage people to be responsible for their chemical usage. I liked the idea of one lady's suggestion to use TBS DAB-Out, maybe that is an answer and I compliment her on her seeking a solution to the problem. Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 At 02:57 AM 6/13/2006, you wrote: >Hi, >I agree, there are obviously limitations as to what should be thrown down >the sink, but not all labs have methods of disposal such as companies to >remove and dispose of. Or the money to invest in dab removal systems. >I have worked as a BMS in a number of labs, and I certainly do not know of >many labs who can capture and contain all their waste formalin whilst >doing grossing/cut up, contain the waste whilst performing special stains, >what happens to the xylene, alcohol, silver, and even the dab, peroxide >etc waste gathered whilst doing immuno manually? How about some of the >bleaches and detergents used to clean labs, and the disinfectants used as >infection control? The list goes on....... > >The method I mentioned as far as I know has been used by a number of labs >for a long time, not just specifically Ventana. Joe you mentioned you had >used it, but perhaps in larger quantities. > >In a perfect, ideal, environmentally friendly world, we would not have >solvents etc, but we don't, and finding companies to remove lab waste who >do not charge high premiums that NHS labs, can afford is difficult. > >Certainly a discussion that needs to be addressed with regulations and >specific guidelines put firmly in place. > >Regards >Emma > > >F-----Original Message-----rom: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >histonet-request@lists.utsouthwestern.edu >Sent: Monday, June 12, 2006 7:23 PM >To: histonet@lists.utsouthwestern.edu >Subject: Histonet Digest, Vol 31, Issue 18[Scanned] > > > > > >-- >No virus found in this outgoing message. >Checked by AVG Free Edition. >Version: 7.1.394 / Virus Database: 268.8.3/359 - Release Date: 08/06/2006 > > > > >------------------------------ > >Message: 3 >Date: Mon, 12 Jun 2006 10:24:36 -0700 (PDT) >From: heidi gordon >Subject: [Histonet] formalin disposal >To: histonet@lists.utsouthwestern.edu >Message-ID: <20060612172436.91656.qmail@web30715.mail.mud.yahoo.com> >Content-Type: text/plain; charset=iso-8859-1 > >I work at a facility that processes its own tissue. >We keep the tissue blocks in a bucket of formalin >until it is put on the processor at the end of the >day. I have been disposing of it at the end of each >day. I am wondering how often most facilities dispose >of this formalin. Everyday? When it looks dirty? > >__________________________________________________ >Do You Yahoo!? >Tired of spam? Yahoo! Mail has the best spam protection around >http://mail.yahoo.com > > > > > > > > >Emma, > >Is this what Ventana advises? Labs should check with their local waste >water treatment plant before TREATING anything or putting "diluted" DAB >down the drain. If laboratories are generating this kind of waste on a >weekly basis, this adds up over time. In general, waste water plants do not >like this and EPA does monitor water around here. Montana has discharge >rules (maybe for larger industries) but our city does not want medicines, >household cleaners, solvents, fertilizers, pesticides, oil, etc, flushed >down the drains and warn residents frequently about this in their water bills. > >Wouldn't it be better to collect and have it hauled away for proper >chemical disposal than add even "minute" amounts of a potential carcinogen >to our water supply. We use very little DAB in our lab, but all >chromogens AEC, DAB, permanent red, etc, (very low volume usage) are >collected for chemical waste pickup and proper disposal. > >An interesting sidelight, tested water wells in parts of Montana now have >traces of sunscreen chemical, medicines, herbicides, nitrates and >nitrites. So much for the "pristine" environment and pure water out in >the Wild Wild West /Rocky Mountain region. Drink beer and brush you teeth >with it too, it may be safer when you visit some Montana dude ranch! > >Gayle Callis HTL, HT, MT(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University >Bozeman MT 59717 > > > > > > > >Message: 1 > >Date: Fri, 9 Jun 2006 12:29:00 -0500 > >From: jhaviland@mdanderson.org > >Subject: [Histonet] Vectastain elite kits > >To: histonet@lists.utsouthwestern.edu > >Message-ID > > > >Dear Histonetters: > >I have the Dako unit. It separates the hazardous/non-hazardous >waste. The DAB goes into a 20L carboy that is then hauled off-site for >disposal. > > >Tom McNemar, HT(ASCP) >Histology Co-ordinator >Licking Memorial Health Systems >(740) 348-4163 >(740) 348-4166 >tmcnemar@lmhealth.org >www.LMHealth.org > > > >Hi Tom >Today we became proud owners of a brand new Dako unit and I had that >exact thought in mind - what to do with a 20 litre bottle of >dab-contaminated waste....every couple of weeks - here in the UAE we >have little choice as there are no federal laws and no off-site waste >companies to cart waste off even for a fee. >I am stuck until someone in authority writes the book of rules. >I just know that one of you will go 'gasp!shock!horror'! and insist that >I be pro-active - I am - some of you know me to be 'tenacious' - this is >correct - but even that has not helped me here. Advice has been offered, >best practice quoted, OH&S quoted, internet sites accessed, printed, >handed over in report form, promises are made by those in authority and >then broken. >We stockpile safely off site and wait >This is true for ALL toxic waste in this laboratory, as well as the rest >of the labs, together with mercury filled blood pressure cuffs, some >broken, 'expired' rat poison, old mercury thermometers, 'unknown' >unlabelled chemicals from shut down labs...some scary stuff >But - I digress - back to the DAB....and how to manage the growing >volume of dab waste..... >Any suggestions.....??? >Anyone.....??? > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom >McNemar >Sent: Monday, June 12, 2006 4:59 PM >To: Joe Nocito; histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] DAB disposal > >I have the Dako unit. It separates the hazardous/non-hazardous waste. >The DAB goes into a 20L carboy that is then hauled off-site for >disposal. > > >Tom McNemar, HT(ASCP) >Histology Co-ordinator >Licking Memorial Health Systems >(740) 348-4163 >(740) 348-4166 >tmcnemar@lmhealth.org >www.LMHealth.org > >-- >No virus found in this outgoing message. >Checked by AVG Free Edition. >Version: 7.1.394 / Virus Database: 268.8.3/359 - Release Date: 08/06/2006 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From J_Lahti <@t> Comcast.net Tue Jun 13 11:22:42 2006 From: J_Lahti <@t> Comcast.net (J Lahti) Date: Tue Jun 13 11:22:46 2006 Subject: [Histonet] POSITION AVAILABLE, NORTHBROOK, IL Message-ID: Histotech needed for Mohs surgical practice in Northbrook, IL FT/PT position available for a motivated individual in an expanding practice. Please reply to J_Lahti@Comcast.net or fax resumes to 847-272-4434. Sincerely, James Lahti, M.D., M.P.H. From aboris <@t> agh.org Tue Jun 13 11:46:08 2006 From: aboris <@t> agh.org (Anthony F. Boris) Date: Tue Jun 13 11:46:19 2006 Subject: [Histonet] why Message-ID: One thing that we found to help with the screened cassettes is to set the cassette on a paper towel during grossing and adding formalin to the cassette. Let it flow thru to the towel before closing. This seems to help the fluids enter when we put them into the bucket. And double check that they do not float up. You may have to give them a tap to displace that bubble. Since we have started this a year ago, we have only had a couple of problems. Tony -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Mon 6/12/2006 7:45 PM To: Santana, Diane; histonet@lists.utsouthwestern.edu Cc: Subject: Re: [Histonet] why Diane, do you the screened cassettes? I ran about 25 GI blocks this morning that sat fixing since Saturday and I had to reprocess 2 of them. This happens every so often and it still puzzles me. Sorry I don't have an answer, but you're not alone with this. There I said it, ( in public too) I don't have all the answers. Joe ----- Original Message ----- From: "Santana, Diane" To: Sent: Monday, June 12, 2006 11:40 AM Subject: [Histonet] why > Hi > I am looking for some ideas to help with a problem I am still having with > processing my GI bx's. What would cause my tissue to be raw? Not all, not > some, only 1 or 2 pieces. > Example if I have a case with 4 bx's, maybe only 1 is raw, the other 3 are > beautiful. All my solutions test at the right % and temperatures are fine. > Question again, why does 1 piece of tissue come out raw, when the other > pieces are fine? > thanks in advance, > Diane Santana > PMA > Haverhill, Mass. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.394 / Virus Database: 268.8.3/361 - Release Date: 6/11/2006 > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From EJones <@t> Ventanamed.com Tue Jun 13 11:57:17 2006 From: EJones <@t> Ventanamed.com (Emma JONES) Date: Tue Jun 13 11:57:27 2006 Subject: More on Re: [Histonet] RE: DAB disposal[Scanned] Message-ID: Absolutely agree with you. The less waste thrown down the sink the = better.=20 You will notice that Joe said he used the same method I mentioned for = his Dako waste. Maybe they recommended it, or perhaps like myself = (excuse me, Joe for assuming here) it is a method he has heard of and = used for a number of years, for me I knew of it a long time before = joining, or even knowing about Ventana. Various countries have a variety of methods of disposal, some can afford = disposal companies, some cannot, some have guidelines set up for = disposal, some do not. Within countries there is variation, and yes cost = is an issue. There are certainly kits that can be purchased for use with our = instruments for disposal of waste, but that is up to the individual lab. = Ultimately their preferred method of disposal is determined by their = local guidelines. =20 I too would prefer to see more fixed guidelines, but with such = lab/country variation will this be possible? If you have any recommendations please share.=20 Best regards Emma -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu]=20 Sent: Tuesday, June 13, 2006 5:15 PM To: Emma JONES; Histonet@lists.utsouthwestern.edu Subject: More on Re: [Histonet] RE: DAB disposal[Scanned] There has already been a great deal of discussion about this problem, = and=20 specific guidelines are often in place and implemented, even in remote=20 areas and for small(or large) laboratories. Have you done a Histonet=20 Archive search on messages concerning this issue, it is there and been=20 going on for a LONG time - well over 10 years or more - this is not a = new=20 issue. Our laboratory has been well aware of DAB treatment for disposal = and did it for a time, but opted NOT to do it long before we were = required=20 by regulation to cease but collect. This treatment never addressed = the=20 by product issue i.e. what happens to the DAB after chemical=20 treatment. Are the byproducts going to be just as bad or worse? If a laboratory can afford any kind of expensive immunostaining = instrument=20 such you sell, or even if they do manual IHC or other routine stains, = then=20 they should be able to afford collection/disposal services or seek such = a=20 service. We are a very small user of these compounds you mention i.e DAB, silver, = any heavy metal compounds (chromic acid) even if only 25 mls of the = stuff -=20 it is collected for disposal. Solvents never go down the drain. As for = bleaches, detergents, and disinfectants, wastewater plants do allow=20 certain things but these are also common to households, not just=20 laboratories. As far as I am concerned and there are a lot of others who might=20 agree, ALL labs, no matter if they are the "little guy" need to address = and implement chemical waste collection and disposal. The only time = this=20 could change is an exemption in a locale but laboratories need to find = out=20 if an exemption is in place before the dumping. Once again, users = beware -=20 check out what is allowed for your area. I'm curious - you never said if this is your company's stand for DAB=20 disposal or if it is just your comment. Does Ventana advise/tell people = to=20 treat their DAB wastes for drain dumping? It certainly would not be a = good=20 policy to do so and does not encourage people to be responsible for = their=20 chemical usage. I liked the idea of one lady's suggestion to use TBS DAB-Out, maybe = that=20 is an answer and I compliment her on her seeking a solution to the = problem. Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 --=20 No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.8.3/359 - Release Date: = 08/06/2006 =20 From gcallis <@t> montana.edu Tue Jun 13 12:42:36 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Jun 13 12:42:47 2006 Subject: [Histonet] DAB disposal continued In-Reply-To: References: Message-ID: <6.0.0.22.1.20060613112808.01af7968@gemini.msu.montana.edu> The method for DAB disposal was written up in the first edition of Dapson and Dapson Hazardous Waste book. I am not sure what is in their new edition. NSH may also have guidelines for DAB disposal from their Safety committee. I think we will see more and more guidelines, it has happened in our city/county/state/USA in the last year or so, and even for households. They hold local waste chemical disposal for households biyearly, but university is under the gun, and we abide by some very strict rules at all times. The cost may be an issue, but it may cost more when one is fined!! Unfortunately, that is something that can happen and when we least suspect it will happen. It is not nice to live under the threat of punishment. It is nice to know the kits are available - it would have been nice to know this when your first message came out sans treatment suggestions - a company should always strongly advise using the kits along with chemical safety, and that should be regardless of cost to avoid drain dumping of DAB waste. This is what I wondered about from your initial message and glad to hear Ventana has such thing available or can recommend a kit if they do not have one manufactured for them. It is a problem, but we have only seen the tip of the iceberg - we don't like to be regulated to death, but chemicaled to death isn't much better. At 10:57 AM 6/13/2006, you wrote: >Absolutely agree with you. The less waste thrown down the sink the better. > >You will notice that Joe said he used the same method I mentioned for his >Dako waste. Maybe they recommended it, or perhaps like myself (excuse me, >Joe for assuming here) it is a method he has heard of and used for a >number of years, for me I knew of it a long time before joining, or even >knowing about Ventana. > >Various countries have a variety of methods of disposal, some can afford >disposal companies, some cannot, some have guidelines set up for disposal, >some do not. Within countries there is variation, and yes cost is an issue. >There are certainly kits that can be purchased for use with our >instruments for disposal of waste, but that is up to the individual lab. >Ultimately their preferred method of disposal is determined by their local >guidelines. > >I too would prefer to see more fixed guidelines, but with such lab/country >variation will this be possible? > > >If you have any recommendations please share. > >Best regards >Emma Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From tilycyn <@t> vetmed.auburn.edu Tue Jun 13 13:26:26 2006 From: tilycyn <@t> vetmed.auburn.edu (Cindy Tily) Date: Tue Jun 13 13:26:39 2006 Subject: [Histonet] DAB waste Message-ID: <7.0.1.0.0.20060613131732.017a47a8@vetmed.auburn.edu> Hi Everyone, I rarely write in, but about this topic I feel strongly. Never should a well known carcinogen such as DAB be put down the drain,no matter how dilute (and yes, Ventana reps will tell you the very dilute DAB in the waste bottle is ok to poor down the drain). In this country (the USA) lack of resources and/or money is also a poor excuse (if you can afford using the Ventana system you can afford to have waste hauled off--no matter how many states it must travel). I try to convince folks this way-if that drain pipe went from your workplace, through the waste treatment plant, directly to your home would you allow your children/grandchildren/any loved one to drink it? Sorry for the rant but ignorance and excuses make me crazy! From lloyd.3 <@t> osu.edu Tue Jun 13 13:27:41 2006 From: lloyd.3 <@t> osu.edu (Mary Lloyd) Date: Tue Jun 13 13:28:11 2006 Subject: [Histonet] detecting sphingomyelin Message-ID: <6.0.0.22.2.20060613140613.01984448@pop.service.ohio-state.edu> Hello everyone, My name is Molly Rosebush and I am a resident in oral pathology at OSU working in Mary's lab. We recently received a biopsy from a patient with Niemann-Pick disease. The hallmark of this disorder is accumulation of sphingomyelin in "foam-like" cells within tissues. My question is this: Is there a histochemical or immunohistochemical method/technique for staining sphingomyelin in formalin-fixed tissue? We don't really see any "foam" cells in the specimen, but would like to be able to somehow detect sphingomyelin (if present) in the tissue. Thank you in advance for any help you can provide! --Molly From Heather.A.Harper <@t> pcola.med.navy.mil Tue Jun 13 13:23:06 2006 From: Heather.A.Harper <@t> pcola.med.navy.mil (Heather.A.Harper@pcola.med.navy.mil) Date: Tue Jun 13 13:28:25 2006 Subject: [Histonet] Who makes... Message-ID: Thanks for the input on blades. I'm trying to get free samples on some different blades but I do not know who makes DuraEdge. Can somebody give me a name, or or number I can call to ask for a sample of these blades. Thanks again. What would we do without histonetting. I find out more info on here than I can shake a lamb's tail to:-) Heather A. Harper Naval Hospital From POWELL_SA <@t> Mercer.edu Tue Jun 13 13:38:15 2006 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Tue Jun 13 13:38:52 2006 Subject: [Histonet] Who makes... In-Reply-To: Message-ID: <01M3KVSYQSDU8WXWSP@Macon2.Mercer.edu> Crescent makes them but a number of vendors sell them. Go to this link for the vendors http://www.crescentblades.com/our_products/distributor.asp Shirley Powell -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heather.A.Harper@pcola.med.navy.mil Sent: Tuesday, June 13, 2006 1:23 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Who makes... Thanks for the input on blades. I'm trying to get free samples on some different blades but I do not know who makes DuraEdge. Can somebody give me a name, or or number I can call to ask for a sample of these blades. Thanks again. What would we do without histonetting. I find out more info on here than I can shake a lamb's tail to:-) Heather A. Harper Naval Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SHARON.OSBORN <@t> SPCORP.COM Tue Jun 13 13:56:23 2006 From: SHARON.OSBORN <@t> SPCORP.COM (Osborn, Sharon) Date: Tue Jun 13 13:57:33 2006 Subject: [Histonet] RE: High Profile Blades Message-ID: <9A919A5D70313A4D9C56A025710874080C72EE@kenmsg40.us.schp.com> Heather, The best blade I have currently found is the Rchard-Alen Scientific Edge-Rite High Profile Microtome Blades. They come 50 to a pack. Cat# 4275H. I received a trial pack at a state/regional or national meeting (probably state/regional) and it sat around for a few years. Then, when I was having a particularly difficult time with current blades on some mouse guts and bones, I pulled these out and tried them. Immediately, I asked for some to be ordered and have not looked back. Ask the vendor for a sample or order a pack. Oh, they are also less expensive then what we were using! Fisher Scientific, uh.......Thermo-Shandon Fisher Scientific et. al. carries them routinely. Sharon Osborn DNAX, SP BioPharma Palo Alto, CA Date: Tue, 13 Jun 2006 10:21:38 -0500 From: Heather.A.Harper@pcola.med.navy.mil Subject: [Histonet] High Profile Micrtome blades To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain I have a Leica Microtome 2235. Currently I am using the Leica 818 high profile blades, and they are terrible. They become dull immediately, slowing down my cutting, because it seems I am changing the blade for every block I cut. Does anyone know of a good high profile blade? I was told the high profile blade by DuraEdge is good. Any feedback would be appreciated. Heather A. Harper ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From lmezo <@t> statlab.com Tue Jun 13 14:10:29 2006 From: lmezo <@t> statlab.com (Laura Kinney) Date: Tue Jun 13 14:10:41 2006 Subject: [Histonet] Who makes... In-Reply-To: <01M3KVSYQSDU8WXWSP@Macon2.Mercer.edu> Message-ID: <005e01c68f1d$0968bb40$0903a8c0@statlab.com> Hi, My name is Laura from StatLab Medical Producs. We carry the DuraEdge blades I would be happy to send you a sample!!! I do need your contact info, phone, address, etc. If you would like to give me a call or e-mail me I would be happy to help!!! Thanks, Laura M. Kinney (972) 315-5824 Direct (800) 442-3573 X 289 VM lkinney@statlab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shirley Powell Sent: Tuesday, June 13, 2006 1:38 PM To: Heather.A.Harper@pcola.med.navy.mil; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Who makes... Crescent makes them but a number of vendors sell them. Go to this link for the vendors http://www.crescentblades.com/our_products/distributor.asp Shirley Powell -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heather.A.Harper@pcola.med.navy.mil Sent: Tuesday, June 13, 2006 1:23 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Who makes... Thanks for the input on blades. I'm trying to get free samples on some different blades but I do not know who makes DuraEdge. Can somebody give me a name, or or number I can call to ask for a sample of these blades. Thanks again. What would we do without histonetting. I find out more info on here than I can shake a lamb's tail to:-) Heather A. Harper Naval Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mward <@t> wfubmc.edu Tue Jun 13 14:31:56 2006 From: mward <@t> wfubmc.edu (Martha Ward) Date: Tue Jun 13 14:32:02 2006 Subject: [Histonet] DAB waste Message-ID: <61135F0455D33347B5AAE209B903A3041485443F@EXCHVS2.medctr.ad.wfubmc.edu> We recently went through this disposal issue at our institution and we had the waste from our Ventana sent off for analysis. The report came back that, at least for our area and state, that it was OK for us to dispose of the waste down the drain. Martha Ward Wake Forest University Baptist Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy Tily Sent: Tuesday, June 13, 2006 2:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DAB waste Hi Everyone, I rarely write in, but about this topic I feel strongly. Never should a well known carcinogen such as DAB be put down the drain,no matter how dilute (and yes, Ventana reps will tell you the very dilute DAB in the waste bottle is ok to poor down the drain). In this country (the USA) lack of resources and/or money is also a poor excuse (if you can afford using the Ventana system you can afford to have waste hauled off--no matter how many states it must travel). I try to convince folks this way-if that drain pipe went from your workplace, through the waste treatment plant, directly to your home would you allow your children/grandchildren/any loved one to drink it? Sorry for the rant but ignorance and excuses make me crazy! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Tue Jun 13 15:21:00 2006 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Tue Jun 13 15:20:50 2006 Subject: [Histonet] DAB waste In-Reply-To: <61135F0455D33347B5AAE209B903A3041485443F@EXCHVS2.medctr.ad.wfubmc.edu> Message-ID: <200606132020.k5DKKevW020415@chip.viawest.net> I agree that Dab should not go down the drain but what are we supposed to do when we contact our institutions waste disposal department (they are the ones who are hauling this away) and they say they checked with the water department for our area and they say put it down the drain (the health and safety people stopped taken it from us), should we contract ourselves with someone outside the institution (I doubt if they would allow this)???? Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martha Ward Sent: Tuesday, June 13, 2006 12:32 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] DAB waste We recently went through this disposal issue at our institution and we had the waste from our Ventana sent off for analysis. The report came back that, at least for our area and state, that it was OK for us to dispose of the waste down the drain. Martha Ward Wake Forest University Baptist Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy Tily Sent: Tuesday, June 13, 2006 2:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DAB waste Hi Everyone, I rarely write in, but about this topic I feel strongly. Never should a well known carcinogen such as DAB be put down the drain,no matter how dilute (and yes, Ventana reps will tell you the very dilute DAB in the waste bottle is ok to poor down the drain). In this country (the USA) lack of resources and/or money is also a poor excuse (if you can afford using the Ventana system you can afford to have waste hauled off--no matter how many states it must travel). I try to convince folks this way-if that drain pipe went from your workplace, through the waste treatment plant, directly to your home would you allow your children/grandchildren/any loved one to drink it? Sorry for the rant but ignorance and excuses make me crazy! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Tue Jun 13 15:38:14 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Jun 13 15:38:25 2006 Subject: Compliments on disposal of RE: [Histonet] DAB waste In-Reply-To: <61135F0455D33347B5AAE209B903A3041485443F@EXCHVS2.medctr.ad .wfubmc.edu> References: <61135F0455D33347B5AAE209B903A3041485443F@EXCHVS2.medctr.ad.wfubmc.edu> Message-ID: <6.0.0.22.1.20060613143551.01b4b878@gemini.msu.montana.edu> Martha, My compliments on finding out what you were allowed to do - you went through the proper channels, and an example on how to do it for your area and state. At 01:31 PM 6/13/2006, you wrote: >We recently went through this disposal issue at our institution and we >had the waste from our Ventana sent off for analysis. The report came >back that, at least for our area and state, that it was OK for us to >dispose of the waste down the drain. > >Martha Ward >Wake Forest University Baptist Medical Center Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 From sjt2104 <@t> columbia.edu Tue Jun 13 16:01:19 2006 From: sjt2104 <@t> columbia.edu (Sebastien Thuault) Date: Tue Jun 13 16:01:09 2006 Subject: [Histonet] RE: DAB disposal Message-ID: Just to attract you attention on the fact that bleach may not be the best method to neutralise DAB. The following procedure was found in the histonet archives: Re: DAB disposal and "mutagenicity" << Previous Message | Next Message >> From: "J. A. Kiernan" (by way of histonet) To: histonet Reply-To: Content-Type: text/plain; charset="us-ascii" ---------------------------------------------------------------------------- ---- On Mon, 28 Dec 1998, A. Erickson wrote: > Could someone post the recipe/instructions for the dichromate/sulfuric > acid treatment for DAB? Thanks! It's actually a permanganate, not a dichromate treatment. The procedure for acid permanganate oxidation of spent DAB is as follows. The measurements need not be very accurate. An acid permanganate solution is made by dissolving 4 g KMnO4 in 100 ml of dilute sulphuric acid (made by adding 15 ml conc. H2SO4 slowly and carefully to 85 ml of water). This solution is stable. (My experience is that it's very good at cementing in place the glass stoppers or screw caps of bottles containing it. Add the solution for disposal to an excess of acidified permanganate and leave overnight (in a fume hood if the solution contained chloride ions, because these will end up as chlorine). Next day, neutralize with sodium hydroxide (carefully; the temperature will rise) and filter. Leave the filter paper to dry in the funnel, then put it in a plastic bag for disposal. If you have a large volume of DAB solution, carefully add sulphuric acid (150 ml for each litre) and then dissolve solid potassium permanganate (40 g for each litre). Reference: Lunn, G & Sansone, EB (1990). Destruction of Hazardous Chemicals in the Laboratory. New York: Wiley Interscience. John A. Kiernan, Department of Anatomy & Cell Biology, The University of Western Ontario, LONDON, Canada N6A 5C1 (kiernan@uwo.ca) Sebastien Thuault, PhD Center for Neurobiology and Behavior Columbia University PI Annex/Kolb Institute 1051 Riverside Drive New York, NY 10032 Phone: +1-212-543-5037 Fax: +1-212-795-7997 From Amanda.Garcia <@t> TriadHospitals.com Tue Jun 13 16:12:12 2006 From: Amanda.Garcia <@t> TriadHospitals.com (Garcia, Amanda) Date: Tue Jun 13 16:12:17 2006 Subject: [Histonet] Leasing histology equipment Message-ID: <8B63039C9DF4554C8FDBF31235F0E148018707A5@CPRTEVS02.triadhospitals.net> Does anyone know of a company that leases histology equipment? Thanks in advance for your help. > Amanda (Amy) Garcia > Histology/Pathology > College Station Medical Center > (979) 680-5372 office > (979) 696-5446 fax > *Mailto:amanda.garcia@triadhospitals.com > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the e-mail or any > of its attachments, please be advised that you have received this > e-mail in error and that any use, dissemination, distribution, > forwarding, printing, or copying of this e-mail or any attached files > is strictly prohibited. If you have received this e-mail in error, > please immediately purge it and all attachments and notify the sender > by reply e-mail or contact the sender at the number listed. > > > From ken.turner <@t> stonebow.otago.ac.nz Tue Jun 13 16:12:35 2006 From: ken.turner <@t> stonebow.otago.ac.nz (Ken Turner) Date: Tue Jun 13 16:12:44 2006 Subject: [Histonet] Microwave processor Message-ID: <7.0.1.0.0.20060614090943.01d945d8@stonebow.otago.ac.nz> Hi everyone, Does anyone have any experience using the Pathos microwave processor (Milestone Medical)? I would be interested in any comments you may have. Thanks, Ken. Kenneth W Turner NZCS, BA(Hons), Dip.Teach. Senior Teaching Fellow Director Histology Service Unit Department of Pathology Dunedin School of Medicine e-mail ken.turner@stonebow.otago.ac.nz University of Otago Phone: (03) 479 7135 or (03) 479-7152 New Zealand Fax: (03) 479-7136 From bills <@t> icpmr.wsahs.nsw.gov.au Tue Jun 13 16:14:51 2006 From: bills <@t> icpmr.wsahs.nsw.gov.au (Bill Sinai) Date: Tue Jun 13 16:15:09 2006 Subject: [Histonet] why In-Reply-To: Message-ID: <002801c68f2e$6904fd00$0ecd080a@wsahs.nsw.gov.au> One way to eliminate this problem is to put a drop of something like tween in your formalin or whatever solution you store the cassttes in prior to processing or the first solution on the processor. This reduces the problem of surface tension of fluids across the small holes. Bill Sinai Chief Hospital Scientist Tissue Pathology ICPMR Westmead Hospital Westmead NSW 2145 Phone 02 9845 7774 Fax 02 9687 2330 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anthony F. Boris Sent: Wednesday, 14 June 2006 2:46 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] why One thing that we found to help with the screened cassettes is to set the cassette on a paper towel during grossing and adding formalin to the cassette. Let it flow thru to the towel before closing. This seems to help the fluids enter when we put them into the bucket. And double check that they do not float up. You may have to give them a tap to displace that bubble. Since we have started this a year ago, we have only had a couple of problems. Tony -----Original Message----- From: Joe Nocito [mailto:jnocito@satx.rr.com] Sent: Mon 6/12/2006 7:45 PM To: Santana, Diane; histonet@lists.utsouthwestern.edu Cc: Subject: Re: [Histonet] why Diane, do you the screened cassettes? I ran about 25 GI blocks this morning that sat fixing since Saturday and I had to reprocess 2 of them. This happens every so often and it still puzzles me. Sorry I don't have an answer, but you're not alone with this. There I said it, ( in public too) I don't have all the answers. Joe ----- Original Message ----- From: "Santana, Diane" To: Sent: Monday, June 12, 2006 11:40 AM Subject: [Histonet] why > Hi > I am looking for some ideas to help with a problem I am still having with > processing my GI bx's. What would cause my tissue to be raw? Not all, not > some, only 1 or 2 pieces. > Example if I have a case with 4 bx's, maybe only 1 is raw, the other 3 are > beautiful. All my solutions test at the right % and temperatures are fine. > Question again, why does 1 piece of tissue come out raw, when the other > pieces are fine? > thanks in advance, > Diane Santana > PMA > Haverhill, Mass. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.394 / Virus Database: 268.8.3/361 - Release Date: 6/11/2006 > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. Sydney West Area Health Service (SWAHS) uses virus scanning software but excludes any liability for viruses contained in any email or attachment. This email may contain privileged and confidential information intended only for the use of the addressees named above. If you are not the intended recipient of this email, you are hereby notified that any use, dissemination, distribution, or reproduction of this email is prohibited. If you have received this email in error, please notify SWAHS immediately. Any views expressed in this email are those of the individual sender except where the sender expressly and with authority states them to be the views of SWAHS. From Joyce.Rush <@t> sjmcmn.org Tue Jun 13 16:15:47 2006 From: Joyce.Rush <@t> sjmcmn.org (Rush, Joyce) Date: Tue Jun 13 16:15:52 2006 Subject: [Histonet] DAB waste Message-ID: <2337362E8548BE4C85EE5DD5D526B0857444FB@sjw3smail2.SJMCMN.ORG> Interesting the difference state to state. We had our analysis done and everything from our stainer is deemed hazardous and must be disposed of as hazardous. Joyce Joyce A. Rush, BS, MT(ASCP) Laboratory Manager St Joseph's Medical Center 523 North Third Street Brainerd, MN 56401 Office:218-828-7500 Fax: 218-828-7510 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Martha Ward Sent: Tuesday, June 13, 2006 14:32 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] DAB waste We recently went through this disposal issue at our institution and we had the waste from our Ventana sent off for analysis. The report came back that, at least for our area and state, that it was OK for us to dispose of the waste down the drain. Martha Ward Wake Forest University Baptist Medical Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cindy Tily Sent: Tuesday, June 13, 2006 2:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] DAB waste Hi Everyone, I rarely write in, but about this topic I feel strongly. Never should a well known carcinogen such as DAB be put down the drain,no matter how dilute (and yes, Ventana reps will tell you the very dilute DAB in the waste bottle is ok to poor down the drain). In this country (the USA) lack of resources and/or money is also a poor excuse (if you can afford using the Ventana system you can afford to have waste hauled off--no matter how many states it must travel). I try to convince folks this way-if that drain pipe went from your workplace, through the waste treatment plant, directly to your home would you allow your children/grandchildren/any loved one to drink it? Sorry for the rant but ignorance and excuses make me crazy! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet DISCLAIMER: This email and any files transmitted with it are confidential and intended solely for the use of histonet@lists.utsouthwestern.edu. If you have received this email in error please notify Rush, Joyce and destroy/delete the message. Please note that any views or opinions presented in this email are solely those of the Author and do not necessarily represent those of the company. Finally, the recipient should check this email and any attachments for the presence of viruses. The Medical Center accepts no liability for any damage caused by any virus transmitted by this email. St. Joseph's Medical Center, 523 N. 3rd street, Brainerd, MN 56401 From POWELL_SA <@t> Mercer.edu Tue Jun 13 17:28:03 2006 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Tue Jun 13 17:28:41 2006 Subject: [Histonet] FW: Histology Supervisor at Atlanta VA Medical Center Message-ID: <01M3L3TV823K8WXYDT@Macon2.Mercer.edu> I am posting for a friend. Please do not respond to me, go to the link below for more information. Subject: Histology Supervisor at Atlanta VA Medical Center Importance: High PLEASE PASS THIS MESSAGE ALONG Opening for Histology Supervisor at Atlanta VA medical Center. Well compensated job in modern well equipped lab with modest work load and strong career growth potential. Application has to be completed and submitted on line by June 20, 2006 Go to usajobs.gov and search under Decatur OR Click on this link: http://jobsearch.usajobs.opm.gov/getjob.asp?JobID=44017211 &AVSDM=2006%2D06%2D12+21%3A49%3A40&Logo=0&q=decatur&FedEmp=N&sort=rv&vw=d&br d=3876&ss=0&FedPub=Y&SUBMIT1.x=0&SUBMIT1.y=0 From Shirley.Chu <@t> moldev.com Tue Jun 13 17:34:14 2006 From: Shirley.Chu <@t> moldev.com (Shirley Chu) Date: Tue Jun 13 17:34:36 2006 Subject: [Histonet] CA Delegates to the HOD at NSH in Phoenix Message-ID: <056AFE945F2C1F4292A1F8EBA9B84B5B713555@MI8NYCMAIL15.Mi8.com> Members of the CSH are needed to represent our state society in the HOD held during NSH meeting in Phoenix AZ. To qualify as a delegate, you must be a current member to both NSH and CSH one (1) year prior to the HOD meeting. The HOD will meet on Wed., Sept 13th at 7pm. For further information or to volunteer for this position, please contact: Shirley Chu at 510-675-6260 or e-mail: shirley.chu@moldev.com. Thanks. Shirley Chu Applications Scientist Molecular Devices Corp 510-675-6260 From sbanwait <@t> buckinstitute.org Tue Jun 13 18:11:40 2006 From: sbanwait <@t> buckinstitute.org (Surita Banwait) Date: Tue Jun 13 18:11:49 2006 Subject: [Histonet] mouse embryo slides Message-ID: Hello Does anyone know where I can buy mouse embryo sections- preferably E8 to E18 ? The only place I found online was EMD biosciences, and they discontinued them last year. I would like to use them for immunohistochemistry, preferably Bouin's fixed Paraffin Embedded tissue. Thanks -Surita Surita Banwait Morphology & Imaging Core Research Associate II Buck Institute for Age Research 8001 Redwood Blvd. Novato, CA 94945 415-209-2221 sbanwait@buckinstitute.org From jnocito <@t> satx.rr.com Tue Jun 13 18:56:32 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Jun 13 18:56:38 2006 Subject: More on Re: [Histonet] RE: DAB disposal References: <6.0.0.22.1.20060613083149.01b52008@gemini.msu.montana.edu> Message-ID: <007101c68f44$ff893cd0$0b69ce44@yourxhtr8hvc4p> please remember I asked about the Ventana DAB disposal. That's the point. All the money is going to pay Ventana. I don't have any extra money for disposal. I opted for my raise to be spread around to all my techs so they could get a little more. Which meant that all my 10 techs got a five cent raise. Once in a while, they'll help me take out the trash and clean the restrooms. I hope no one notices that I've been diluting the hand soap to a 1:10 dilution or that I've been separating the two-ply toilet paper to make it last longer. Ya gotta do what ya gotta do. Sorry, couldn't resist. Had a long meeting with my medical director, CEO and Ventana rep today. For those who know the joke, I was like the lady who tried to return the toaster in K-Mart and kept raising my arms yelling "pinch my n--ples, pinch my n--ples". I told the joke to my immuno tech and I thought she was going to an accident she was laughing so hard. And you think I'm funny on the Histonet. Try working with me. We have a couple of boxes of Depends just in case. Joe ----- Original Message ----- From: "Gayle Callis" To: "Emma JONES" ; Sent: Tuesday, June 13, 2006 11:15 AM Subject: More on Re: [Histonet] RE: DAB disposal > There has already been a great deal of discussion about this problem, and > specific guidelines are often in place and implemented, even in remote > areas and for small(or large) laboratories. Have you done a Histonet > Archive search on messages concerning this issue, it is there and been > going on for a LONG time - well over 10 years or more - this is not a new > issue. Our laboratory has been well aware of DAB treatment for disposal > and did it for a time, but opted NOT to do it long before we were required > by regulation to cease but collect. This treatment never addressed the > by product issue i.e. what happens to the DAB after chemical treatment. > Are the byproducts going to be just as bad or worse? > > If a laboratory can afford any kind of expensive immunostaining instrument > such you sell, or even if they do manual IHC or other routine stains, then > they should be able to afford collection/disposal services or seek such a > service. > > We are a very small user of these compounds you mention i.e DAB, silver, > any heavy metal compounds (chromic acid) even if only 25 mls of the > stuff - it is collected for disposal. Solvents never go down the drain. > As for bleaches, detergents, and disinfectants, wastewater plants do > allow certain things but these are also common to households, not just > laboratories. > > As far as I am concerned and there are a lot of others who might agree, > ALL labs, no matter if they are the "little guy" need to address and > implement chemical waste collection and disposal. The only time this > could change is an exemption in a locale but laboratories need to find out > if an exemption is in place before the dumping. Once again, users > beware - check out what is allowed for your area. > > I'm curious - you never said if this is your company's stand for DAB > disposal or if it is just your comment. Does Ventana advise/tell people > to treat their DAB wastes for drain dumping? It certainly would not be a > good policy to do so and does not encourage people to be responsible for > their chemical usage. > > I liked the idea of one lady's suggestion to use TBS DAB-Out, maybe that > is an answer and I compliment her on her seeking a solution to the > problem. > > Gayle Callis HTL, HT, MT(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University > Bozeman MT 59717 > > > > > At 02:57 AM 6/13/2006, you wrote: > > >>Hi, >>I agree, there are obviously limitations as to what should be thrown down >>the sink, but not all labs have methods of disposal such as companies to >>remove and dispose of. Or the money to invest in dab removal systems. >>I have worked as a BMS in a number of labs, and I certainly do not know of >>many labs who can capture and contain all their waste formalin whilst >>doing grossing/cut up, contain the waste whilst performing special stains, >>what happens to the xylene, alcohol, silver, and even the dab, peroxide >>etc waste gathered whilst doing immuno manually? How about some of the >>bleaches and detergents used to clean labs, and the disinfectants used as >>infection control? The list goes on....... >> >>The method I mentioned as far as I know has been used by a number of labs >>for a long time, not just specifically Ventana. Joe you mentioned you had >>used it, but perhaps in larger quantities. >> >>In a perfect, ideal, environmentally friendly world, we would not have >>solvents etc, but we don't, and finding companies to remove lab waste who >>do not charge high premiums that NHS labs, can afford is difficult. >> >>Certainly a discussion that needs to be addressed with regulations and >>specific guidelines put firmly in place. >> >>Regards >>Emma >> >> >>F-----Original Message-----rom: histonet-bounces@lists.utsouthwestern.edu >>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >>histonet-request@lists.utsouthwestern.edu >>Sent: Monday, June 12, 2006 7:23 PM >>To: histonet@lists.utsouthwestern.edu >>Subject: Histonet Digest, Vol 31, Issue 18[Scanned] >> >> >> >> >> >>-- >>No virus found in this outgoing message. >>Checked by AVG Free Edition. >>Version: 7.1.394 / Virus Database: 268.8.3/359 - Release Date: 08/06/2006 >> >> >> >> >>------------------------------ >> >>Message: 3 >>Date: Mon, 12 Jun 2006 10:24:36 -0700 (PDT) >>From: heidi gordon >>Subject: [Histonet] formalin disposal >>To: histonet@lists.utsouthwestern.edu >>Message-ID: <20060612172436.91656.qmail@web30715.mail.mud.yahoo.com> >>Content-Type: text/plain; charset=iso-8859-1 >> >>I work at a facility that processes its own tissue. >>We keep the tissue blocks in a bucket of formalin >>until it is put on the processor at the end of the >>day. I have been disposing of it at the end of each >>day. I am wondering how often most facilities dispose >>of this formalin. Everyday? When it looks dirty? >> >>__________________________________________________ >>Do You Yahoo!? >>Tired of spam? Yahoo! Mail has the best spam protection around >>http://mail.yahoo.com >> >> >> >> >> >> >> >> >>Emma, >> >>Is this what Ventana advises? Labs should check with their local waste >>water treatment plant before TREATING anything or putting "diluted" DAB >>down the drain. If laboratories are generating this kind of waste on a >>weekly basis, this adds up over time. In general, waste water plants do >>not >>like this and EPA does monitor water around here. Montana has discharge >>rules (maybe for larger industries) but our city does not want medicines, >>household cleaners, solvents, fertilizers, pesticides, oil, etc, flushed >>down the drains and warn residents frequently about this in their water >>bills. >> >>Wouldn't it be better to collect and have it hauled away for proper >>chemical disposal than add even "minute" amounts of a potential carcinogen >>to our water supply. We use very little DAB in our lab, but all >>chromogens AEC, DAB, permanent red, etc, (very low volume usage) are >>collected for chemical waste pickup and proper disposal. >> >>An interesting sidelight, tested water wells in parts of Montana now have >>traces of sunscreen chemical, medicines, herbicides, nitrates and >>nitrites. So much for the "pristine" environment and pure water out in >>the Wild Wild West /Rocky Mountain region. Drink beer and brush you >>teeth >>with it too, it may be safer when you visit some Montana dude ranch! >> >>Gayle Callis HTL, HT, MT(ASCP) >>Research Histopathology Supervisor >>Veterinary Molecular Biology >>Montana State University >>Bozeman MT 59717 >> >> >> >> >> >> >> >>Message: 1 >> >>Date: Fri, 9 Jun 2006 12:29:00 -0500 >> >>From: jhaviland@mdanderson.org >> >>Subject: [Histonet] Vectastain elite kits >> >>To: histonet@lists.utsouthwestern.edu >> >>Message-ID >> >> >> >>Dear Histonetters: >> >>I have the Dako unit. It separates the hazardous/non-hazardous waste. >>The DAB goes into a 20L carboy that is then hauled off-site for disposal. >> >> >>Tom McNemar, HT(ASCP) >>Histology Co-ordinator >>Licking Memorial Health Systems >>(740) 348-4163 >>(740) 348-4166 >>tmcnemar@lmhealth.org >>www.LMHealth.org >> >> >> >>Hi Tom >>Today we became proud owners of a brand new Dako unit and I had that >>exact thought in mind - what to do with a 20 litre bottle of >>dab-contaminated waste....every couple of weeks - here in the UAE we >>have little choice as there are no federal laws and no off-site waste >>companies to cart waste off even for a fee. >>I am stuck until someone in authority writes the book of rules. >>I just know that one of you will go 'gasp!shock!horror'! and insist that >>I be pro-active - I am - some of you know me to be 'tenacious' - this is >>correct - but even that has not helped me here. Advice has been offered, >>best practice quoted, OH&S quoted, internet sites accessed, printed, >>handed over in report form, promises are made by those in authority and >>then broken. >>We stockpile safely off site and wait >>This is true for ALL toxic waste in this laboratory, as well as the rest >>of the labs, together with mercury filled blood pressure cuffs, some >>broken, 'expired' rat poison, old mercury thermometers, 'unknown' >>unlabelled chemicals from shut down labs...some scary stuff >>But - I digress - back to the DAB....and how to manage the growing >>volume of dab waste..... >>Any suggestions.....??? >>Anyone.....??? >> >>-----Original Message----- >>From: histonet-bounces@lists.utsouthwestern.edu >>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tom >>McNemar >>Sent: Monday, June 12, 2006 4:59 PM >>To: Joe Nocito; histonet@lists.utsouthwestern.edu >>Subject: RE: [Histonet] DAB disposal >> >>I have the Dako unit. It separates the hazardous/non-hazardous waste. >>The DAB goes into a 20L carboy that is then hauled off-site for >>disposal. >> >> >>Tom McNemar, HT(ASCP) >>Histology Co-ordinator >>Licking Memorial Health Systems >>(740) 348-4163 >>(740) 348-4166 >>tmcnemar@lmhealth.org >>www.LMHealth.org >> >>-- >>No virus found in this outgoing message. >>Checked by AVG Free Edition. >>Version: 7.1.394 / Virus Database: 268.8.3/359 - Release Date: 08/06/2006 >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.394 / Virus Database: 268.8.3/362 - Release Date: 6/12/2006 > > From jnocito <@t> satx.rr.com Tue Jun 13 19:01:48 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Jun 13 19:01:54 2006 Subject: More on Re: [Histonet] RE: DAB disposal[Scanned] References: Message-ID: <007701c68f45$bc0b9970$0b69ce44@yourxhtr8hvc4p> Emma, you're excused (kidding). I need to contact the company hauling off my xylene/alcohol waste. Other than using the DAB OUT, which I heard clogs the DAB OUT fast because of the liquid coverslip, I'm trying to think of a way to reduce the amount of liquid being placed into the barrel. We run two machines three, maybe four times a day. that's a lot of waste. Ooops, gotta go, time to clean the restrooms Joe ----- Original Message ----- From: "Emma JONES" To: "Gayle Callis" Cc: Sent: Tuesday, June 13, 2006 11:57 AM Subject: RE: More on Re: [Histonet] RE: DAB disposal[Scanned] Absolutely agree with you. The less waste thrown down the sink the better. You will notice that Joe said he used the same method I mentioned for his Dako waste. Maybe they recommended it, or perhaps like myself (excuse me, Joe for assuming here) it is a method he has heard of and used for a number of years, for me I knew of it a long time before joining, or even knowing about Ventana. Various countries have a variety of methods of disposal, some can afford disposal companies, some cannot, some have guidelines set up for disposal, some do not. Within countries there is variation, and yes cost is an issue. There are certainly kits that can be purchased for use with our instruments for disposal of waste, but that is up to the individual lab. Ultimately their preferred method of disposal is determined by their local guidelines. I too would prefer to see more fixed guidelines, but with such lab/country variation will this be possible? If you have any recommendations please share. Best regards Emma -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: Tuesday, June 13, 2006 5:15 PM To: Emma JONES; Histonet@lists.utsouthwestern.edu Subject: More on Re: [Histonet] RE: DAB disposal[Scanned] There has already been a great deal of discussion about this problem, and specific guidelines are often in place and implemented, even in remote areas and for small(or large) laboratories. Have you done a Histonet Archive search on messages concerning this issue, it is there and been going on for a LONG time - well over 10 years or more - this is not a new issue. Our laboratory has been well aware of DAB treatment for disposal and did it for a time, but opted NOT to do it long before we were required by regulation to cease but collect. This treatment never addressed the by product issue i.e. what happens to the DAB after chemical treatment. Are the byproducts going to be just as bad or worse? If a laboratory can afford any kind of expensive immunostaining instrument such you sell, or even if they do manual IHC or other routine stains, then they should be able to afford collection/disposal services or seek such a service. We are a very small user of these compounds you mention i.e DAB, silver, any heavy metal compounds (chromic acid) even if only 25 mls of the stuff - it is collected for disposal. Solvents never go down the drain. As for bleaches, detergents, and disinfectants, wastewater plants do allow certain things but these are also common to households, not just laboratories. As far as I am concerned and there are a lot of others who might agree, ALL labs, no matter if they are the "little guy" need to address and implement chemical waste collection and disposal. The only time this could change is an exemption in a locale but laboratories need to find out if an exemption is in place before the dumping. Once again, users beware - check out what is allowed for your area. I'm curious - you never said if this is your company's stand for DAB disposal or if it is just your comment. Does Ventana advise/tell people to treat their DAB wastes for drain dumping? It certainly would not be a good policy to do so and does not encourage people to be responsible for their chemical usage. I liked the idea of one lady's suggestion to use TBS DAB-Out, maybe that is an answer and I compliment her on her seeking a solution to the problem. Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 -- No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.8.3/359 - Release Date: 08/06/2006 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.8.3/362 - Release Date: 6/12/2006 From louise.renton <@t> gmail.com Wed Jun 14 02:46:55 2006 From: louise.renton <@t> gmail.com (louise renton) Date: Wed Jun 14 02:47:02 2006 Subject: [Histonet] decal and ihc Message-ID: dear all, on pain of revisiting stuff that may or may not have been addressed before- I have just done some immuno on FFPE tissue that had been decalcified in a decal mix of 10% formic , 10% HCl and 1% Na Citrate. Duration in acidic soln is unknown, as these blocks date from before my employment here. On performing immuno, I found that the staining is "muddy" and non specific. Is this the usual consequence of decalcification or did I unwittingly do something wrong?. The antibody used has been optimised on normal FFPE tissue, and worked fine, with clean and crisp staining. I was always under the impression that decalcification destroyed antigenicity and thus caused less staining rather than more??!! Having given this scenario, is there anything I can try to improve matters? (poly rabbit antibody incubated overnight @4deg C. ABC amplification and DAB chromogen. No pretreatment performed, washes in PBS with tween) Best regards Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From vbaker60 <@t> yahoo.com Wed Jun 14 07:07:27 2006 From: vbaker60 <@t> yahoo.com (Victoria Baker) Date: Wed Jun 14 07:07:32 2006 Subject: [Histonet] Leasing histology equipment In-Reply-To: <8B63039C9DF4554C8FDBF31235F0E148018707A5@CPRTEVS02.triadhospitals.net> Message-ID: <20060614120727.84398.qmail@web52507.mail.yahoo.com> Amanda, There are leasing companies available if you want to purchase equipment from a particular manufacturer. At one of the institutions I worked at we had a leasing company that we worked with for much of our capital purchases. The leasing company purchases the equipment and "owns" it, your company then pays the leasing company X amount of payments until the conditions have been met for you to "own" it. You may need to check with your financial people if they have done this or if they already have one. Another avenue is to see if the the company you want to buy the equipment from has a plan that will allow you to lease the equipment. I might be off base, but with all the competition for "big" purchases of equipment many sales reps will go the extra yard to get the sale. I'm not a big fan of leasing as to me the only one who makes money in the end is the leasing company, but that just my opinion. One other thing that I ran into when it came into leasing of equipment is that many times it meant getting refurbished vs. new equipment. I wish you luck in your search. Vikki Baker --- "Garcia, Amanda" wrote: > Does anyone know of a company that leases histology > equipment? Thanks > in advance for your help. > > > Amanda (Amy) Garcia > > Histology/Pathology > > College Station Medical Center > > (979) 680-5372 office > > (979) 696-5446 fax > > *Mailto:amanda.garcia@triadhospitals.com > > > > This email and any files transmitted with it may > contain PRIVILEGED or > > CONFIDENTIAL information and may be read or used > only by the intended > > recipient. If you are not the intended recipient > of the e-mail or any > > of its attachments, please be advised that you > have received this > > e-mail in error and that any use, dissemination, > distribution, > > forwarding, printing, or copying of this e-mail or > any attached files > > is strictly prohibited. If you have received this > e-mail in error, > > please immediately purge it and all attachments > and notify the sender > > by reply e-mail or contact the sender at the > number listed. > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From gayle.brosnanwatters <@t> sru.edu Wed Jun 14 07:22:28 2006 From: gayle.brosnanwatters <@t> sru.edu (gayle brosnanwatters) Date: Wed Jun 14 07:22:36 2006 Subject: [Histonet] Leasing histology equipment Message-ID: <8172F14C39FCAF4E99E266F6756A368303260C94@msfexch01.srunet.sruad.edu> If you do a google for used lab equipement, you'll find it, Amanda, because I ran across some when I was looking for a place to sell mine. I can't remember who it was, but you should find the same ones I did. Gayle Brosnan-Watters -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Garcia, Amanda Sent: Tue 6/13/2006 5:12 PM To: histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] Leasing histology equipment Does anyone know of a company that leases histology equipment? Thanks in advance for your help. > Amanda (Amy) Garcia > Histology/Pathology > College Station Medical Center > (979) 680-5372 office > (979) 696-5446 fax > *Mailto:amanda.garcia@triadhospitals.com > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the e-mail or any > of its attachments, please be advised that you have received this > e-mail in error and that any use, dissemination, distribution, > forwarding, printing, or copying of this e-mail or any attached files > is strictly prohibited. If you have received this e-mail in error, > please immediately purge it and all attachments and notify the sender > by reply e-mail or contact the sender at the number listed. > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Wed Jun 14 07:39:24 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Wed Jun 14 07:36:33 2006 Subject: [Histonet] Trichrome staining and fibrin Message-ID: Well, that's telling me:-) All these years, the success I've had with primary mercury fixation (I had the original article once), and lack of success with anything other was entirely due to something else, the techs washing too much in one instance and little enough in another. Who would have thought it. However it may explain my observation that "At times it works and at times not." Actually, the only time you can be confident that you are looking at fibrin, is when it is in strands - easily recognised in H&E. To call anything else fibrin on any tinctorial stain is something akin to a leap of faith. But - I'll get you back re. your "bees can't fly" thing. Take this/that: :-) The "science has proved that bees can't fly" urban myth originated in a 1934 book by entomologist Antoine Magnan, who discussed a mathematical equation by Andre Sainte-Lague, an engineer. The equation proved that the maximum lift for an aircraft's wings could not be achieved at equivalent speeds of a bee. I.e., an airplane the size of a bee, moving as slowly as a bee, could not fly. Although this did not mean a bee can't fly (which after all does not have stationary wings like the posited teensy aircraft), nevertheless the idea that Magnan's book said bees oughtn't be able to fly began to spread. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Bryan Hewlett [mailto:bhewlett@cogeco.ca] Sent: 13 June 2006 17:14 To: Marshall Terry Dr, Consultant Histopathologist; Timo V?is?nen; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Trichrome staining and fibrin Terry, Actually NOT! True, Lendrum's original papers(1962) recommended fixation in picro-mercuric alcohol for 3 weeks (a tad unnecessary that!). However, both Prof. Lendrum and Bill Slidders also successfully used formaldehyde fixed material that was treated in Bouin immediately prior to staining. The key to all of the Masson Type trichromes on formaldehyde-fixed tissue, is to use a pretreatment in picric acid. This re-aligns the reactive side chains on the proteins, so that there is a predominance of basic amino groups and hence maximal binding of anionic dyes. Mercury fixation is simply not necessary! Nor is Zinc substitution. (see Troubleshooting Histology stains: Horobin and Bancroft 1998, page 129) The lack of consistency, in obtaining good red colour, is due variability in the staining procedure (the post dye water rinses), NOT the fixation. Once this is addressed, the inconsistency goes away. That is true for the original Masson, Picro-Mallory (all variants), MSB and Masson 44/41. Achieving consistent good red colour with formaldehyde fixed material is easy, if the staining mechanisms are understood and those unnecessary water rinses removed. Been doing it for over 40 years! Bryan (Engineers can prove that the bumble bee simply cannot fly. However, the bumble bee doesn't know that and flies anyway) ----- Original Message ----- From: "Marshall Terry Dr, Consultant Histopathologist" To: "Bryan Hewlett" ; "Timo V?is?nen" ; Sent: Tuesday, June 13, 2006 11:27 AM Subject: RE: [Histonet] Trichrome staining and fibrin Bryan, You know full well that MSB required initial staining in mercury. I know that zinc is an adequate replacement. Attempting to get a consistent good red colour with formalin fixed material is futile, as is post-fixation. At times it works and at times not. IMO, Masson is useless for fibrin. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Bryan Hewlett [mailto:bhewlett@cogeco.ca] Sent: 13 June 2006 15:52 To: Timo V?is?nen; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Trichrome staining and fibrin Timo, Try the Lendrum MSB trichrome variant, it was designed to demonstrate fibrin. I will send the method via a separate e-mail. Bryan ----- Original Message ----- From: "Timo V?is?nen" To: Sent: Tuesday, June 13, 2006 10:33 AM Subject: [Histonet] Trichrome staining and fibrin > Hello all, > > I have struggled with getting Masson Trichrome (Goldner) staining to work > with > kidney biopsies. The problem is that our pathologist does not get positive > fibrin staining to glomeruli in diseased kidneys. Those areas of the > glomeruli > that should contain fibrin, at least according to the pathologist, are > green > (Lichtgrun) and not red, as they should be, whatever I do. I have tried to > enhance the staining with Bouin's fixative (+56C) pretreatment but it did > not > change the overall situation. However, the red stain was more intense in > skin > samples containing fibrin (formalin fixed). I have also tried another > Trichrome > staining with Crocein Scarlet 7B without any luck. In our lab kidney > biopsies > are routinely fixed with alcoholic Bouin's. As far as I know it should be > compatible with the Masson protocol we use. Any ideas? Any good protocols > that > I could try? > > Thank's in advance, > > Timo > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Margaret.Perry <@t> sdstate.edu Wed Jun 14 09:08:36 2006 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Wed Jun 14 09:08:42 2006 Subject: [Histonet] substrates Message-ID: We are trying to do double staining and are looking for two different colors of substrate and we can't use DAB due to melanin. Does anyone know of a green stain that is xylene compatible? Vector has Nova Red and a blue color but we use hematoxylin as a counterstain. It would mean an extra step for us to counterstain in Methyl Green We use a DAKO autostainer. Margaret Perry HT(ASCP) South Dakota State University Animal Research and Diagnostic Lab Brookings SD 57007 Margaret.Perry@sdstate.edu From Paula.F.Conlon <@t> Lahey.org Wed Jun 14 09:16:10 2006 From: Paula.F.Conlon <@t> Lahey.org (Conlon, Paula F.) Date: Wed Jun 14 09:16:21 2006 Subject: [Histonet] toe nails Message-ID: Hi Histonetters, does anyone have a good procedure for sectioning nails and keeping them on the slides during h&e staining and pas staining?Most of the time our sections wash off. Thanks for your help. See our web page at http://www.lahey.org for a full directory of Lahey sites, staff, services and career opportunities. THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS ADDRESSED. IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended recipient, your use of this message for any purpose is strictly prohibited. If you have received this communication in error, please delete the message and notify the sender so that we may correct our records. From rjbuesa <@t> yahoo.com Wed Jun 14 09:23:19 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 14 09:23:24 2006 Subject: [Histonet] substrates In-Reply-To: Message-ID: <20060614142319.7044.qmail@web61216.mail.yahoo.com> Margaret: Confronted with that situation I would eliminate the melanine better. Oxidize it with 0.1% potassium permanganate for 2 hours; wash and remove the oxidized melanine with 0.3% oxalic acid followed by washing. The section should be free of melanine pigment/colour afterwards and you can use DAB (for brown) and DAB + Ni salts for dark blue as double substrates. Hope this will help you! Ren? J. "Perry, Margaret" wrote: We are trying to do double staining and are looking for two different colors of substrate and we can't use DAB due to melanin. Does anyone know of a green stain that is xylene compatible? Vector has Nova Red and a blue color but we use hematoxylin as a counterstain. It would mean an extra step for us to counterstain in Methyl Green We use a DAKO autostainer. Margaret Perry HT(ASCP) South Dakota State University Animal Research and Diagnostic Lab Brookings SD 57007 Margaret.Perry@sdstate.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From rjbuesa <@t> yahoo.com Wed Jun 14 09:25:13 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 14 09:25:20 2006 Subject: [Histonet] toe nails In-Reply-To: Message-ID: <20060614142513.15660.qmail@web61219.mail.yahoo.com> Paula: I am forwarding privately a summary on Toenails I prepared recently that I hope will answer your questions. Ren? J. "Conlon, Paula F." wrote: Hi Histonetters, does anyone have a good procedure for sectioning nails and keeping them on the slides during h&e staining and pas staining?Most of the time our sections wash off. Thanks for your help. See our web page at http://www.lahey.org for a full directory of Lahey sites, staff, services and career opportunities. THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS ADDRESSED. IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended recipient, your use of this message for any purpose is strictly prohibited. If you have received this communication in error, please delete the message and notify the sender so that we may correct our records. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From cpomajzl <@t> cpllabs.com Wed Jun 14 09:41:48 2006 From: cpomajzl <@t> cpllabs.com (Chris Pomajzl) Date: Wed Jun 14 09:37:24 2006 Subject: [Histonet] toe nails References: Message-ID: <002e01c68fc0$b2fb6330$26fca8c0@CSP> Good question. We have dealt with this issue for many years, and we have come up with a solution that has worked for some time. First of all, sections are picked up on "+" coated slides that have been smeared with egg albumin. We separate eggs from the grocery store and keep a stock of the egg white albumin in a bottle. Maybe 100ml at a time, keep it refrigerated. When cutting nails, put 2-3 drops of albumin on the slide and smear to cover all of the glass. We cut 3 sections of the nail specimen. 1 for H&E, 1 for PAS, and 1 extra for back-up. We bake the slides at 55'C overnight, and then at 70'C for 20 minutes prior to staining the following day. This seems to work very well for us. ----- Original Message ----- From: "Conlon, Paula F." To: "Histonet (E-mail)" Sent: Wednesday, June 14, 2006 9:16 AM Subject: [Histonet] toe nails Hi Histonetters, does anyone have a good procedure for sectioning nails and keeping them on the slides during h&e staining and pas staining?Most of the time our sections wash off. Thanks for your help. See our web page at http://www.lahey.org for a full directory of Lahey sites, staff, services and career opportunities. THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS ADDRESSED. IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended recipient, your use of this message for any purpose is strictly prohibited. If you have received this communication in error, please delete the message and notify the sender so that we may correct our records. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mbmphoto <@t> gmail.com Wed Jun 14 10:11:41 2006 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Wed Jun 14 10:10:59 2006 Subject: [Histonet] ScyTek's serum free pro-block protein Message-ID: To anyone who using ScyTek's serum free pro-block protein, please let me know what you think of it. In IHC, do you (only) use it as a diluent for the antibodies or also use it in the washes? Any information you can provide will be greatly appreciated. Yours Maria Bartola Mejia UCSF Depart. of Neurosurgery San Francisco, CA 94103 From aboris <@t> agh.org Wed Jun 14 10:51:21 2006 From: aboris <@t> agh.org (Anthony F. Boris) Date: Wed Jun 14 10:51:39 2006 Subject: [Histonet] toe nails Message-ID: We use charged slides from Premiere. keep in 65 degree oven for 30 minutes and stain. We have not had any fall off since we switched to these slides. When cutting you can use a 5% solution of KOh for a "surface decal". This breaks down the keratin a little bit (same stuff as in NAIR). Helps to get decent sectioning. Tony -----Original Message----- From: Chris Pomajzl [mailto:cpomajzl@cpllabs.com] Sent: Wed 6/14/2006 10:41 AM To: HISTONET Cc: Subject: Re: [Histonet] toe nails Good question. We have dealt with this issue for many years, and we have come up with a solution that has worked for some time. First of all, sections are picked up on "+" coated slides that have been smeared with egg albumin. We separate eggs from the grocery store and keep a stock of the egg white albumin in a bottle. Maybe 100ml at a time, keep it refrigerated. When cutting nails, put 2-3 drops of albumin on the slide and smear to cover all of the glass. We cut 3 sections of the nail specimen. 1 for H&E, 1 for PAS, and 1 extra for back-up. We bake the slides at 55'C overnight, and then at 70'C for 20 minutes prior to staining the following day. This seems to work very well for us. ----- Original Message ----- From: "Conlon, Paula F." To: "Histonet (E-mail)" Sent: Wednesday, June 14, 2006 9:16 AM Subject: [Histonet] toe nails Hi Histonetters, does anyone have a good procedure for sectioning nails and keeping them on the slides during h&e staining and pas staining?Most of the time our sections wash off. Thanks for your help. See our web page at http://www.lahey.org for a full directory of Lahey sites, staff, services and career opportunities. THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS ADDRESSED. IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended recipient, your use of this message for any purpose is strictly prohibited. If you have received this communication in error, please delete the message and notify the sender so that we may correct our records. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From drgeoffreywhite <@t> yahoo.com Wed Jun 14 10:52:10 2006 From: drgeoffreywhite <@t> yahoo.com (Geoffrey White) Date: Wed Jun 14 10:52:16 2006 Subject: [Histonet] Manual cover slip method - DiscoverSlip? Message-ID: <20060614155210.68428.qmail@web36513.mail.mud.yahoo.com> Dear all, at the time our research group is looking for new manual method for cover our slides during the hybridization. In the past we used standard glass cover slips but we are not really satisfied with this method. Just I saw at Biocompare a new method with the name DiscoverSlip. This technology looks very interesting. Have any work with DiscoverSlip or saw this technology working? Thanks Geoffrey __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From susan.wells <@t> bms.com Wed Jun 14 11:01:42 2006 From: susan.wells <@t> bms.com (Susan Q Wells) Date: Wed Jun 14 11:05:00 2006 Subject: [Histonet] substrates In-Reply-To: <20060614142319.7044.qmail@web61216.mail.yahoo.com> References: <20060614142319.7044.qmail@web61216.mail.yahoo.com> Message-ID: <449032E6.8030300@bms.com> You may lose your epitopes with this procedure, if so 10% H202 in PBS for 30 minutes may lighten the melanin pigment enough to view your chromagen. I recently used Vulcan Fast Red and Bajoran Purple from Biocare Medical with great success where melanin pigment was present. Sue Wells HT(ASCP), QIHC Rene J Buesa wrote: >Margaret: > Confronted with that situation I would eliminate the melanine better. Oxidize it with 0.1% potassium permanganate for 2 hours; wash and remove the oxidized melanine with 0.3% oxalic acid followed by washing. The section should be free of melanine pigment/colour afterwards and you can use DAB (for brown) and DAB + Ni salts for dark blue as double substrates. > Hope this will help you! > Ren? J. > >"Perry, Margaret" wrote: > We are trying to do double staining and are looking for two different >colors of substrate and we can't use DAB due to melanin. Does anyone >know of a green stain that is xylene compatible? Vector has Nova Red >and a blue color but we use hematoxylin as a counterstain. It would >mean an extra step for us to counterstain in Methyl Green > >We use a DAKO autostainer. > > > >Margaret Perry HT(ASCP) > >South Dakota State University > >Animal Research and Diagnostic Lab > >Brookings SD 57007 > >Margaret.Perry@sdstate.edu > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > __________________________________________________ >Do You Yahoo!? >Tired of spam? Yahoo! Mail has the best spam protection around >http://mail.yahoo.com >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From mtitford <@t> aol.com Wed Jun 14 11:14:59 2006 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Wed Jun 14 11:15:12 2006 Subject: [Histonet] Sphingomyelin Message-ID: <8C85DE4D15F5C0F-DE4-1999@mblk-r15.sysops.aol.com> Mary Lloyd and Dr Rosebush ask about sphingomyelin: I am no expert on sphingomyelin (underlined!) but had to suffer through classes on the subject during histotechnology training in England. As a histotechnologist, when you think about sphingomyelin, you think about methods that will stain normal myelin like Sudan black, PAS and acid hematein. In an excellent book edited by Isobel Filipe and Brian Lake "Histochemistry in Pathology" Churchill Livingstone (1983) there are several references to Neuman Pick disease and histochemical methods to demonstrate sphingomyelin, and cholesterol and gangliosides which can accompany the disease process. Mike Titford USA Pathology Mobile AL USA ________________________________________________________________________ Check out AOL.com today. Breaking news, video search, pictures, email and IM. All on demand. Always Free. From dsnider <@t> shrinenet.org Wed Jun 14 11:15:10 2006 From: dsnider <@t> shrinenet.org (Snider, Deanna) Date: Wed Jun 14 11:15:15 2006 Subject: [Histonet] High profile blades Message-ID: <84BE46B37B314D409C5A17B7BAB022D6980CA7@IDC-EX-VS01.shriners.cc> Message: 10 Date: Tue, 13 Jun 2006 10:21:38 -0500 From: Heather.A.Harper@pcola.med.navy.mil Subject: [Histonet] High Profile Micrtome blades To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain I have a Leica Microtome 2235. Currently I am using the Leica 818 high profile blades, and they are terrible. They become dull immediately, slowing down my cutting, because it seems I am changing the blade for every block I cut. Does anyone know of a good high profile blade? I was told the high profile blade by DuraEdge is good. Any feedback would be appreciated. Heather A. Harper Have you tried the Dura Edge line? They make a very good blade and very cost efficient. They come in regular, ceramic, and teflon caoted edges. I prefer the ceramic edge for my work. I have a contact number if you'd like to request a sample. Deanna Snider HT ASCP Shriners Hospital for Children Research Dept. Cincinnatti, Oh 513-872-6388 CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients. If you are not the intended recipient, (or authorized to receive for the recipient) you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please destroy all copies of this communication and any attachments and contact the sender by reply e-mail or telephone (813) 281-0300. From nair.ashwin <@t> gmail.com Wed Jun 14 11:43:47 2006 From: nair.ashwin <@t> gmail.com (Ashwin Nair) Date: Wed Jun 14 11:43:51 2006 Subject: [Histonet] Mechanical testing-Dallas-Fort Worth Message-ID: Hi, I was wondering if there were people doing tensile and compression testing of tissues and polymers in this forum? Also it would be great if someone could suggest a forum for discussing the same, if this isn't the right forum. I am trying to setup a mechanical tester (Instron Mini44) for compression and tensile testing. I had a few questions pertaining to the use of this machine and whether there were any labs in Dallas Fort Worth area already using this. I would appreciate any help in this area. Thank you. Ashwin From TJJ <@t> Stowers-Institute.org Wed Jun 14 13:01:47 2006 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Wed Jun 14 13:02:13 2006 Subject: [Histonet] Re: Substrates Message-ID: Margaret, This has been discussion on the Histonet before, and while I have not personally done this, I've heard it works: >>Try counterstaining the immuno slides with giemsa rather than performing the bleaching technique. I use the May Grunwald giemsa following DAB. The melanin pigment will stain green and contrasts nicely beside the brown DAB. Dr. H Kamino originally published counterstaining with azure B to color melanin. I learned this trick from her when she was the chief of dermatopathology here at Duke. We routinely use this combination. One note of interest, sometimes the DAB color reaction is enhanced or altered because of prior treatment of the tissue, such as antigen retrieval or enzyme digestion. Jim Burchette Duke Immunopathology<< You might also consider using Vector's Nova Red which works well, provides nice contrast with hematoxylin, and is stable to alcohol and xylene. That way you would only have to change the chromogen and nothing else in your protocol. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From Eric <@t> ategra.com Wed Jun 14 10:02:46 2006 From: Eric <@t> ategra.com (Eric Dye (ext 223)) Date: Wed Jun 14 13:04:49 2006 Subject: [Histonet] N E W list of Histology Openings - Interviewing and Hiring now - June 12 2006 Message-ID: Hi Fellow-Histonetters - is your phone number still phone1>> phone2>> phone3>> ? Below is an updated listing of Histology permanent and temporary jobs I have throughout the country. If you are interested in any of the Histology j o b s listed below or anywhere else - please call me at 800-466-9919 ext 223 or cell - 407-756-5507. Most Histo Tech jobs are permanent full-time dayshift. Here are some of my Newest Histology Jobs: ------------------------------------------------------ 01. Coastal, Georgia (close to S.C.) - HistoTech - Perm - must be A S C P (HT or HTL) - dayshift 02. Southeast Florida - temp - HistoTech 03. Florida, West Coast - both temp & perm openings- Bench Histotech 04. Pennsylvania, Pittsburgh - perm - Bench HistoTech - days 05. Virginia/D.C. Beltway East - Fairfax Area - Perm, Histo Tech / Histo Supervisor << - NEW !! 06. New Hampshire - - both temp & perm openings - Histotech << - NEW !! 07. Washington (State)- Perm - Histotech - days- Mon- Fri << - NEW !! 08. South Carolina - Perm - Supervisor and Bench - Histotech - Mon- Fri << - NEW !! 09. Boston Mass - HistoTech - one Senior HIstoTech, One not so Senior Histotech - days and early AMS << - NEW !! Thank you, Eric Dye-Sr Allied Healthcare Recruiter 800-466-9919 ext 223 or Cell - 407-756-5507 P.S.: Feel free to pass along this email and my phone number to anyone who you think might be interested. P.S.S.: The clients are currently interviewing - and the job will close soon - so if you are interested, please call me today at 1-800-466-9919 ext 229 --------------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing 800 466 9919 ext 223 - voice 407 671 6075 - fax eric@ategra.com To Learn More About Ategra: http://www.ategra.com ---------------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. ---------------------------------------------------------------- ---------------------------------------------------------------- Note: this message is intended for: Fellow-Histonetters at histonet@lists.utsouthwestern.edu ---------------------------------------------------------------- From godsgirlnow <@t> msn.com Wed Jun 14 14:44:58 2006 From: godsgirlnow <@t> msn.com (Roxanne Soto) Date: Wed Jun 14 14:45:49 2006 Subject: [Histonet] JOBS IN CENTRAL FLORIDA Message-ID: Hello everyone, We are a private Lab in Tampa and we are in need of Data Entry people, Lab Assistants, Histotechnicians, and Histotechnologists. We do mostly GA and GU specimens. Please feel free to email me for more information. Thank you Roxanne Soto Clinical Lab Supervisor PRP Tampa, Florida From gcallis <@t> montana.edu Wed Jun 14 15:55:33 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Jun 14 15:55:44 2006 Subject: [Histonet] Person looking for a Cryocut 1800 manual, please contact me Message-ID: <6.0.0.22.1.20060614144953.01b46988@gemini.msu.montana.edu> Would the person looking for a Leica Cryocut 1800 manual please contact me Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From lrichey <@t> u.washington.edu Wed Jun 14 18:15:38 2006 From: lrichey <@t> u.washington.edu (Lori Richey) Date: Wed Jun 14 18:15:53 2006 Subject: [Histonet] substrates In-Reply-To: References: Message-ID: <4490989A.2090200@u.washington.edu> We use Giemsa as a counter stain on tissue with internal melanin pigment. The Giemsa turns the melanin a dark blue green, which allows you to distinguish the brown DAB staining from the internal melanin pigment. This might be an idea. Perry, Margaret wrote: >We are trying to do double staining and are looking for two different >colors of substrate and we can't use DAB due to melanin. Does anyone >know of a green stain that is xylene compatible? Vector has Nova Red >and a blue color but we use hematoxylin as a counterstain. It would >mean an extra step for us to counterstain in Methyl Green > >We use a DAKO autostainer. > > > >Margaret Perry HT(ASCP) > >South Dakota State University > >Animal Research and Diagnostic Lab > >Brookings SD 57007 > >Margaret.Perry@sdstate.edu > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From bhewlett <@t> cogeco.ca Wed Jun 14 20:02:13 2006 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Wed Jun 14 20:02:19 2006 Subject: [Histonet] Trichrome staining and fibrin References: Message-ID: <001401c69017$56e7d7c0$6500a8c0@mainbox> Terry, I figured that one urban myth deserved another! Your comment on the leap of faith with most empirically derived tinctorial stains is certainly very true. But then, Histopathology is full of such leaps. e.g. simple descriptive terms like acidophilia, basophilia etc., which are of course dependent on technique. Another example is the infamous classification "Malignant Fibrous Histiocytoma" very few of which, it now turns out, have anything to do with Histiocytes. The magnitude of that leap of faith is always greatly reduced by using a standardized, reproducible, tinctorial stain with appropriate controls. If validation of these results by confirmation with biochemical analysis, histochemistry, immunohistochemistry or EM etc. follows, then, if it walks like a duck and quacks like a duck, chances are it's a ............! However, when definitive identification of a fibrinoid deposit is necessary, we have always confirmed the presence of fibrin by IHC. Bryan ----- Original Message ----- From: "Marshall Terry Dr,Consultant Histopathologist" To: "Bryan Hewlett" ; "Timo V?is?nen" ; Sent: Wednesday, June 14, 2006 8:39 AM Subject: RE: [Histonet] Trichrome staining and fibrin Well, that's telling me:-) All these years, the success I've had with primary mercury fixation (I had the original article once), and lack of success with anything other was entirely due to something else, the techs washing too much in one instance and little enough in another. Who would have thought it. However it may explain my observation that "At times it works and at times not." Actually, the only time you can be confident that you are looking at fibrin, is when it is in strands - easily recognised in H&E. To call anything else fibrin on any tinctorial stain is something akin to a leap of faith. But - I'll get you back re. your "bees can't fly" thing. Take this/that: :-) The "science has proved that bees can't fly" urban myth originated in a 1934 book by entomologist Antoine Magnan, who discussed a mathematical equation by Andre Sainte-Lague, an engineer. The equation proved that the maximum lift for an aircraft's wings could not be achieved at equivalent speeds of a bee. I.e., an airplane the size of a bee, moving as slowly as a bee, could not fly. Although this did not mean a bee can't fly (which after all does not have stationary wings like the posited teensy aircraft), nevertheless the idea that Magnan's book said bees oughtn't be able to fly began to spread. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Bryan Hewlett [mailto:bhewlett@cogeco.ca] Sent: 13 June 2006 17:14 To: Marshall Terry Dr, Consultant Histopathologist; Timo V?is?nen; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Trichrome staining and fibrin Terry, Actually NOT! True, Lendrum's original papers(1962) recommended fixation in picro-mercuric alcohol for 3 weeks (a tad unnecessary that!). However, both Prof. Lendrum and Bill Slidders also successfully used formaldehyde fixed material that was treated in Bouin immediately prior to staining. The key to all of the Masson Type trichromes on formaldehyde-fixed tissue, is to use a pretreatment in picric acid. This re-aligns the reactive side chains on the proteins, so that there is a predominance of basic amino groups and hence maximal binding of anionic dyes. Mercury fixation is simply not necessary! Nor is Zinc substitution. (see Troubleshooting Histology stains: Horobin and Bancroft 1998, page 129) The lack of consistency, in obtaining good red colour, is due variability in the staining procedure (the post dye water rinses), NOT the fixation. Once this is addressed, the inconsistency goes away. That is true for the original Masson, Picro-Mallory (all variants), MSB and Masson 44/41. Achieving consistent good red colour with formaldehyde fixed material is easy, if the staining mechanisms are understood and those unnecessary water rinses removed. Been doing it for over 40 years! Bryan (Engineers can prove that the bumble bee simply cannot fly. However, the bumble bee doesn't know that and flies anyway) ----- Original Message ----- From: "Marshall Terry Dr, Consultant Histopathologist" To: "Bryan Hewlett" ; "Timo V?is?nen" ; Sent: Tuesday, June 13, 2006 11:27 AM Subject: RE: [Histonet] Trichrome staining and fibrin Bryan, You know full well that MSB required initial staining in mercury. I know that zinc is an adequate replacement. Attempting to get a consistent good red colour with formalin fixed material is futile, as is post-fixation. At times it works and at times not. IMO, Masson is useless for fibrin. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Bryan Hewlett [mailto:bhewlett@cogeco.ca] Sent: 13 June 2006 15:52 To: Timo V?is?nen; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Trichrome staining and fibrin Timo, Try the Lendrum MSB trichrome variant, it was designed to demonstrate fibrin. I will send the method via a separate e-mail. Bryan ----- Original Message ----- From: "Timo V?is?nen" To: Sent: Tuesday, June 13, 2006 10:33 AM Subject: [Histonet] Trichrome staining and fibrin > Hello all, > > I have struggled with getting Masson Trichrome (Goldner) staining to work > with > kidney biopsies. The problem is that our pathologist does not get positive > fibrin staining to glomeruli in diseased kidneys. Those areas of the > glomeruli > that should contain fibrin, at least according to the pathologist, are > green > (Lichtgrun) and not red, as they should be, whatever I do. I have tried to > enhance the staining with Bouin's fixative (+56C) pretreatment but it did > not > change the overall situation. However, the red stain was more intense in > skin > samples containing fibrin (formalin fixed). I have also tried another > Trichrome > staining with Crocein Scarlet 7B without any luck. In our lab kidney > biopsies > are routinely fixed with alcoholic Bouin's. As far as I know it should be > compatible with the Masson protocol we use. Any ideas? Any good protocols > that > I could try? > > Thank's in advance, > > Timo > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From katri <@t> cogeco.ca Wed Jun 14 21:25:41 2006 From: katri <@t> cogeco.ca (Katri Tuomala) Date: Wed Jun 14 21:25:39 2006 Subject: [Histonet] substrates References: <20060614142319.7044.qmail@web61216.mail.yahoo.com> Message-ID: <000601c69022$ff55f8f0$6a9a9618@Katri> Margaret & Rene, Just a caution: the bleaching the melanin before immuno stains may abolish or diminish some antigen/antibody reactions. Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "Rene J Buesa" To: "Perry, Margaret" ; Sent: Wednesday, June 14, 2006 10:23 AM Subject: Re: [Histonet] substrates > Margaret: > Confronted with that situation I would eliminate the melanine better. > Oxidize it with 0.1% potassium permanganate for 2 hours; wash and remove > the oxidized melanine with 0.3% oxalic acid followed by washing. The > section should be free of melanine pigment/colour afterwards and you can > use DAB (for brown) and DAB + Ni salts for dark blue as double substrates. > Hope this will help you! > Ren? J. > > "Perry, Margaret" wrote: > We are trying to do double staining and are looking for two different > colors of substrate and we can't use DAB due to melanin. Does anyone > know of a green stain that is xylene compatible? Vector has Nova Red > and a blue color but we use hematoxylin as a counterstain. It would > mean an extra step for us to counterstain in Methyl Green > > We use a DAKO autostainer. > > > > Margaret Perry HT(ASCP) > > South Dakota State University > > Animal Research and Diagnostic Lab > > Brookings SD 57007 > > Margaret.Perry@sdstate.edu > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brucea <@t> unimelb.edu.au Wed Jun 14 23:30:18 2006 From: brucea <@t> unimelb.edu.au (Bruce Abaloz) Date: Wed Jun 14 23:30:36 2006 Subject: [Histonet] A Call for Help from Melbourne/OZ Message-ID: Dear Histonetters, My name is Scott Sinclair, and I am a PhD student in the Department of Genetics (@ The University of Melbourne/Australia) and am hoping to get your advice on the following: I have been using a fluorescent dye (Zinpyr) to try to image Zinc in the roots of Arabidopsis seedlings/plant, using a confocal microscope. I'm also interested in looking at paraffin sections of roots, therefore wondering if there is a 'fixing' process that will leave the intracellular localisation of Zinc undisturbed while examining sections/Will routine fixation in 10% NBF or 70% ETOH be fine? I know you have to fix sections before looking at protein or DNA/RNA, and was wondering if it can be done for ions also? Regards, Scott Sinclair (sent through Bruce) -- BRUCE ABALOZ PH:61383446282 HISTOLOGIST FAX:61383447909 DEPT. of ZOOLOGY EMAIL: brucea@unimelb.edu.au THE UNIVERSITY Of MELBOURNE. VICTORIA. AUSTRALIA 3010 Nobody Can Make You Feel Inferior Without YOUR Permission - Eleanor Roosevelt DANCE LIKE NO-ONE'S WATCHING Q: What's a specimen? A: An Italian astronaut. From John.Sheppard <@t> Health-Partners.org Thu Jun 15 04:38:08 2006 From: John.Sheppard <@t> Health-Partners.org (John.Sheppard@Health-Partners.org) Date: Thu Jun 15 04:38:15 2006 Subject: [Histonet] Florida HT's Message-ID: Hello Histonetters, I am wondering if anyone out there has recently aquired a Florida license for performing histology lab testing / work. Especially if you used your HT (ASCP) certification with only a high school diploma (the now discontinued route 3) to get your license. Also when applying for the Florida license are the Florida HT and HTL catagories equivalent to the ASCP HT and HTL catagories. The application seems pretty expensive and I do not want to make a mistake. Any information in this area would be greatly appreciated. Thank you for your time John Sheppard HT(ASCP) CONFIDENTIALITY NOTICE: This message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From mcauliff <@t> umdnj.edu Thu Jun 15 09:14:36 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Jun 15 09:14:01 2006 Subject: [Histonet] A Call for Help from Melbourne/OZ In-Reply-To: References: Message-ID: <44916B4C.4040303@umdnj.edu> THE person for zinc is G. Danscher, do a PubMed search.for his name or "detection of zinc". Geoff Bruce Abaloz wrote: > Dear Histonetters, > > My name is Scott Sinclair, and I am a PhD student in the Department of > Genetics (@ The University of Melbourne/Australia) and am hoping to > get your advice on the following: > I have been using a fluorescent dye (Zinpyr) to try to image Zinc in > the roots of Arabidopsis seedlings/plant, using a confocal microscope. > I'm also interested in looking at paraffin sections of roots, > therefore wondering if there is a 'fixing' process that will leave the > intracellular localisation of Zinc undisturbed while examining > sections/Will routine fixation in 10% NBF or 70% ETOH be fine? I know > you have to fix sections before looking at protein or DNA/RNA, and was > wondering if it can be done for ions also? > > Regards, > > Scott Sinclair (sent through Bruce) -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From pathrm35 <@t> adelphia.net Thu Jun 15 09:27:28 2006 From: pathrm35 <@t> adelphia.net (pathrm35@adelphia.net) Date: Thu Jun 15 09:27:32 2006 Subject: [Histonet] online histology programs Message-ID: <16259775.1150381648636.JavaMail.root@web26> Fellow techs, Does anyone know of any online histology programs? Thanks, Ron Martin From JWEEMS <@t> sjha.org Thu Jun 15 09:36:03 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Thu Jun 15 09:35:17 2006 Subject: [Histonet] online histology programs Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD320295FF57@sjhaexc02.sjha.org> Darton College in Albany, GA Contact: Carl Sagasser, BS, HT(ASCP) Educational Coordinator Histotechnology Program Darton College 2400 Gillionville Road Albany, GA 31701 P: (229) 317-6974 F: (229) 317-6682 Cheers! Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of pathrm35@adelphia.net Sent: Thursday, June 15, 2006 10:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] online histology programs Fellow techs, Does anyone know of any online histology programs? Thanks, Ron Martin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From POWELL_SA <@t> Mercer.edu Thu Jun 15 09:46:27 2006 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Thu Jun 15 09:46:56 2006 Subject: [Histonet] online histology programs In-Reply-To: <16259775.1150381648636.JavaMail.root@web26> Message-ID: <01M3NGB8JDAM8WY7E5@Macon2.Mercer.edu> Go to www.darton.edu to the allied health programs. Shirley Powell -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pathrm35@adelphia.net Sent: Thursday, June 15, 2006 9:27 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] online histology programs Fellow techs, Does anyone know of any online histology programs? Thanks, Ron Martin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AGrobe2555 <@t> aol.com Thu Jun 15 10:45:49 2006 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Thu Jun 15 10:46:06 2006 Subject: [Histonet] Neutrophil Staining Message-ID: <36d.5808067.31c2daad@aol.com> Just recently someone posted a protocol for staining neutrophils with DAB. Could someone please direct me to the posting or re-post the protocol. Thank you From liz <@t> premierlab.com Thu Jun 15 11:09:08 2006 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Thu Jun 15 11:07:17 2006 Subject: [Histonet] immuno background staining from agarose cells blocks Message-ID: <000001c69096$083d5850$0300a8c0@Chlipala> Hello All I had prepared some agarose cell blocks that contained bovine embryos and cut unstained slides for a client. I'm not performing the immunos the client is and they experienced alot of background from the agarose during the stainining process. Is there a way to get rid of the background staining or remove the agarose without loosing the embryos. The person that is performing the immunos follows appropriate protocols with blocking steps. Any help would be appreciated . Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 From sluhisto <@t> yahoo.com Thu Jun 15 11:52:36 2006 From: sluhisto <@t> yahoo.com (Histology SLU) Date: Thu Jun 15 11:52:40 2006 Subject: [Histonet] Sirius Red Protocol mouse pancreas Message-ID: <20060615165236.59889.qmail@web51002.mail.yahoo.com> Hello All: I have a dilemma that I hope that someone out there can shed some light on. We have an investigator that has been working with mouse FFPE pancreas. We have been doing the processing, embedding, sectioning, and sirius red staining. Lately, however, we have been getting too much red, more than the collagen is staining. The staining pattern is everywhere!! This is a new development and we have tried the following: 1. Making new reagents 2. Evaluating the processes that the mouse went through prior to necropsy. 3. Did a fixation evaluation to make sure that fixation was not an issue. 4. Multiple techs performing stain We are at a loss to help this investigator and don't want to give up!! We had performed this stain numerous times in the past for this investigator without any problems. Any help you can send my way would be appreciated by myself as well as the investigator. Susan __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From bjessee <@t> omnisonics.com Thu Jun 15 12:22:07 2006 From: bjessee <@t> omnisonics.com (Bret Jessee) Date: Thu Jun 15 12:22:13 2006 Subject: [Histonet] Ex Vivo Arterial Gross Path Stains? Message-ID: I'm looking to do some work using porcine carotid arteries freshly harvested from slaughterhouse animals to assess damage to the vessel walls by intravascular medical devices (guidewires, catheters, thrombectomy and atherectomy devices designed to clear thrombus and plaque). I would like to stain the arteries with water-soluble dyes to detect gross damage to the intimal and medial layers using low magnification binocular microscopy. Would any stains be particularly useful? Best regards, BRET C. Bret Jessee, Ph.D. Director Preclinical Affairs OmniSonics Medical Technologies, Inc. 66 Concord Street, Suite A Wilmington, MA 01887 TEL: (978) 657-9980 x332 FAX: (978) 657-9982 bjessee@omnisonics.com www.omnisonics.com Notice: This e-mail message and any attachments to it may contain legally privileged confidential information. If you are not the intended recipient, you must not review, retransmit, convert to hard copy, copy, use or disseminate this e-mail or any attachments. If you have received this e-mail in error, please immediately notify us by return e-mail or by telephone at 978-657-9980 and delete this message. From gcallis <@t> montana.edu Thu Jun 15 12:34:51 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Jun 15 12:34:58 2006 Subject: [Histonet] Glucose Oxidase blocking for peroxidase, glucose source Message-ID: <6.0.0.22.1.20060615110659.01b3f1a0@gemini.msu.montana.edu> To all using Glucose oxidase endogenous peroxidase blocking, be aware that Sigma has discontinued the beta-D(+) glucose used for this blocking. Some have tried another glucose as a substitute and the peroxidase was not blocked. Since I tout this highly useful peroxidase blocking method, I found a source of the glucose ICN Cat#100953 beta-D Glucose. this contain up to 10% of alpha-isomer, although Sigma's contained only 4%. Hopefully this will not be discontinued as Sigma has done. If there are any doubts, call Sigma Technical Services and ask them for a source of this glucose. Never thought there would be so much difference in a glucose molecule Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From SohrabB1 <@t> wmmcpo.ah.org Thu Jun 15 12:38:33 2006 From: SohrabB1 <@t> wmmcpo.ah.org (Behnaz Sohrab) Date: Thu Jun 15 12:39:15 2006 Subject: [Histonet] Policy Message-ID: Dose any one have a Policy regarding faxing pathology report to other Dr's offices? Behnaz, Histo supervisor From jamie.erickson <@t> abbott.com Thu Jun 15 12:48:11 2006 From: jamie.erickson <@t> abbott.com (Jamie E Erickson) Date: Thu Jun 15 12:48:15 2006 Subject: [Histonet] Re:Sirius Red Protocol mouse pancreas In-Reply-To: <50hjq2$gr3su@viper.abbott.com> Message-ID: Susan, We also do Sirius red staining but of mouse lungs, we use a polarizing light to quantitative collagen deposition. We have not see and appreciable increase in red staining over time. Maximum staining intensity is reach after 90 minutes in the paper by L.C.U. Junqueira Histochemical Journal 1979, 11,447-445. Check the histonet achieves that's where I got most of my information. As far as the staining I would run an old sample to determine if that stains differently then it had in the past, if it does then it's a staining issue if not it's the tissue, it could be an experimental issue. Our investigators usually give us control samples untreated Lung or we can ask for muscle or kidney for a positive control I'd look into the tissue being the issue if you haven't already done that. Hope that helps. Jamie _______________________________ Jamie Erickson Sr. Research Associate Department: DSMP Abbott Bioresearch Center 100 Research Drive Worcester, MA 01605-4341 508-688-3134 FAX: 508-793-4895 e-mail: jamie.erickson@abbott.com From dlcowie <@t> prodigy.net Thu Jun 15 15:19:07 2006 From: dlcowie <@t> prodigy.net (Dawn Cowie) Date: Thu Jun 15 15:19:10 2006 Subject: [Histonet] productivity for pathology transcription personnel Message-ID: <20060615201907.17095.qmail@web81012.mail.mud.yahoo.com> hello histonetters, I am wondering if any of you have any data on productivity levels/expectations for your transcription people that you are willing to share? What quantity of work performed in an average day? Number of lines transcribed? What kind of lines? Microscopic diagnosis or gross descriptions? Any info will be greatly appreciated. thanks, Dawn Cowie AP Director Bon Secours Health Partners Lab Richmond, VA From jzeliadt <@t> msn.com Thu Jun 15 23:50:19 2006 From: jzeliadt <@t> msn.com (JEFF TATUM ZELIADT) Date: Thu Jun 15 23:50:25 2006 Subject: [Histonet] productivity for pathology transcription personnel In-Reply-To: <20060615201907.17095.qmail@web81012.mail.mud.yahoo.com> Message-ID: ______________________________________________________________ From: Dawn Cowie To: histonet Subject: [Histonet] productivity for pathology transcription personnel Date: Thu, 15 Jun 2006 13:19:07 -0700 (PDT) >hello histonetters, > I am wondering if any of you have any data on productivity levels/expectations for your transcription people that you are willing to share? What quantity of work performed in an average day? Number of lines transcribed? What kind of lines? Microscopic diagnosis or gross descriptions? Any info will be greatly appreciated. > thanks, > Dawn Cowie > AP Director > Bon Secours Health Partners Lab > Richmond, VA >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jzeliadt <@t> msn.com Thu Jun 15 23:50:57 2006 From: jzeliadt <@t> msn.com (JEFF TATUM ZELIADT) Date: Thu Jun 15 23:51:01 2006 Subject: [Histonet] Re:Sirius Red Protocol mouse pancreas In-Reply-To: Message-ID: ______________________________________________________________ From: Jamie E Erickson To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re:Sirius Red Protocol mouse pancreas Date: Thu, 15 Jun 2006 13:48:11 -0400 >Susan, > We also do Sirius red staining but of mouse lungs, we use >a polarizing light to quantitative collagen deposition. We have not see >and appreciable increase in red staining over time. Maximum staining >intensity is reach after 90 minutes in the paper by L.C.U. Junqueira >Histochemical Journal 1979, 11,447-445. Check the histonet achieves that's >where I got most of my information. > As far as the staining I would run an old sample to >determine if that stains differently then it had in the past, if it does >then it's a staining issue if not it's the tissue, it could be an >experimental issue. Our investigators usually give us control samples >untreated Lung or we can ask for muscle or kidney for a positive control >I'd look into the tissue being the issue if you haven't already done that. > >Hope that helps. > >Jamie > >_______________________________ >Jamie Erickson >Sr. Research Associate >Department: DSMP >Abbott Bioresearch Center >100 Research Drive >Worcester, MA 01605-4341 >508-688-3134 >FAX: 508-793-4895 >e-mail: jamie.erickson@abbott.com >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jzeliadt <@t> msn.com Thu Jun 15 23:51:40 2006 From: jzeliadt <@t> msn.com (JEFF TATUM ZELIADT) Date: Thu Jun 15 23:51:47 2006 Subject: [Histonet] Policy In-Reply-To: Message-ID: fuckin remove my email from your damn list ______________________________________________________________ From: "Behnaz Sohrab" To: Subject: [Histonet] Policy Date: Thu, 15 Jun 2006 10:38:33 -0700 >Dose any one have a Policy regarding faxing pathology report to other Dr's offices? >Behnaz, Histo supervisor > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jzeliadt <@t> msn.com Thu Jun 15 23:52:18 2006 From: jzeliadt <@t> msn.com (JEFF TATUM ZELIADT) Date: Thu Jun 15 23:52:25 2006 Subject: [Histonet] Glucose Oxidase blocking for peroxidase, glucose source In-Reply-To: <6.0.0.22.1.20060615110659.01b3f1a0@gemini.msu.montana.edu> Message-ID: remove my email from you damn lists fuck head ______________________________________________________________ From: Gayle Callis To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Glucose Oxidase blocking for peroxidase, glucose source Date: Thu, 15 Jun 2006 11:34:51 -0600 >To all using Glucose oxidase endogenous peroxidase blocking, be >aware that Sigma has discontinued the beta-D(+) glucose used for >this blocking. Some have tried another glucose as a substitute and >the peroxidase was not blocked. > >Since I tout this highly useful peroxidase blocking method, I found >a source of the glucose > >ICN Cat#100953 beta-D Glucose. this contain up to 10% of >alpha-isomer, although Sigma's contained only 4%. > >Hopefully this will not be discontinued as Sigma has done. If there >are any doubts, call Sigma Technical Services and ask them for a >source of this glucose. > >Never thought there would be so much difference in a glucose >molecule > > > > > > > >Gayle Callis >MT,HT,HTL(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University - Bozeman >PO Box 173610 >Bozeman MT 59717-3610 >406 994-6367 >406 994-4303 (FAX) > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jzeliadt <@t> msn.com Thu Jun 15 23:53:19 2006 From: jzeliadt <@t> msn.com (JEFF TATUM ZELIADT) Date: Thu Jun 15 23:53:27 2006 Subject: [Histonet] Sirius Red Protocol mouse pancreas In-Reply-To: <20060615165236.59889.qmail@web51002.mail.yahoo.com> Message-ID: you are fuckin pissing me off with all these messages. pull you head out of your ass and remove me from your lists ______________________________________________________________ From: Histology SLU To: "histonet@lists.utsouthwestern.edu" CC: tetriba@slu.edu Subject: [Histonet] Sirius Red Protocol mouse pancreas Date: Thu, 15 Jun 2006 09:52:36 -0700 (PDT) >Hello All: > > I have a dilemma that I hope that someone out there can shed some light on. We have an investigator that has been working with mouse FFPE pancreas. We have been doing the processing, embedding, sectioning, and sirius red staining. Lately, however, we have been getting too much red, more than the collagen is staining. The staining pattern is everywhere!! This is a new development and we have tried the following: > > 1. Making new reagents > 2. Evaluating the processes that the mouse went through prior to necropsy. > 3. Did a fixation evaluation to make sure that fixation was not an issue. > 4. Multiple techs performing stain > > We are at a loss to help this investigator and don't want to give up!! We had performed this stain numerous times in the past for this investigator without any problems. > > Any help you can send my way would be appreciated by myself as well as the investigator. > > Susan > > __________________________________________________ >Do You Yahoo!? >Tired of spam? Yahoo! Mail has the best spam protection around >http://mail.yahoo.com >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dmccaig <@t> ckha.on.ca Fri Jun 16 09:23:30 2006 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Fri Jun 16 09:27:44 2006 Subject: [Histonet] Hp Yellow and Hp blue stain for Hp Message-ID: Is anyone using this stain for Helicobacter pylori using the protocol provided from Anatech. Did you find the times needed modified and were you satisfied with the results. Diana McCaig 519-352-6401 (6604) Charge Technologist, Histology Chatham Kent Heath Alliance 80 Grand Ave West Chatham, Ontario N7L 1B7 From GDawson <@t> dynacaremilwaukee.com Fri Jun 16 09:58:53 2006 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Fri Jun 16 09:59:01 2006 Subject: [Histonet] Who is this MORON? Message-ID: If anyone knows who this foul mouthed little kid is or where I can find him please let me know so I can rip his head off and defecate down his neck. In case he has more than 3 or 4 functional brain cells: to unsubscribe, respond to the histonet with the word "unsubscribe" in the subject line & you'll be removed from the list. Save your potty mouth a bit so you can still suck on your nook while you watch Sesame Street. Glen D. Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of JEFF TATUM ZELIADT Sent: Thursday, June 15, 2006 10:53 PM To: sluhisto@yahoo.com; histonet@lists.utsouthwestern.edu Cc: tetriba@slu.edu Subject: RE: [Histonet] Sirius Red Protocol mouse pancreas you are fuckin pissing me off with all these messages. pull you head out of your ass and remove me from your lists ______________________________________________________________ From: Histology SLU To: "histonet@lists.utsouthwestern.edu" CC: tetriba@slu.edu Subject: [Histonet] Sirius Red Protocol mouse pancreas Date: Thu, 15 Jun 2006 09:52:36 -0700 (PDT) >Hello All: > > I have a dilemma that I hope that someone out there can shed some light on. We have an investigator that has been working with mouse FFPE pancreas. We have been doing the processing, embedding, sectioning, and sirius red staining. Lately, however, we have been getting too much red, more than the collagen is staining. The staining pattern is everywhere!! This is a new development and we have tried the following: > > 1. Making new reagents > 2. Evaluating the processes that the mouse went through prior to necropsy. > 3. Did a fixation evaluation to make sure that fixation was not an issue. > 4. Multiple techs performing stain > > We are at a loss to help this investigator and don't want to give up!! We had performed this stain numerous times in the past for this investigator without any problems. > > Any help you can send my way would be appreciated by myself as well as the investigator. > > Susan > > __________________________________________________ >Do You Yahoo!? >Tired of spam? Yahoo! Mail has the best spam protection around >http://mail.yahoo.com >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TillRenee <@t> uams.edu Fri Jun 16 11:15:56 2006 From: TillRenee <@t> uams.edu (Till, Renee) Date: Fri Jun 16 11:16:22 2006 Subject: [Histonet] where is my stain! Message-ID: <11F927674DEBDC43B960809A7403C5D201379634@MAILPED.ad.uams.edu> I have done Pten on rat mammary gland for several years now with very little problems. My only changes to the protocol have been to add the avidin/biotin blocks and switch to a fab2 secondary, which has all but eliminated the non-specific staining I was getting. The tissues I am working with now, I have worked with others that come from the exact same conditions. In a sense they are almost the controls, the normal animals, to compare to the ones used for the cancer research. I cannot get my pten stain to work. It is the same antibody, same protocol, same everything that I have always used or done to the tissues. I asked the company if they had made any changes to their antibody and was told no. I tried another pten from another company and I get the same results. There are only 5 or so of my 14 tissues that ever have even the slightest hint of a stain. 2 to 3 of those come out "normal", with staining like I would expect, with another couple only having the most minimal staining in spots. The others are completely negative for what I am looking for. Some of them do have a brown artifact like staining around the structures, or in the luminal areas, but that is it. There is absolutely no reason I can think of that these rats would not express pten, and I can't think of anything I've done different. If it is one of my reagents, I don't know why a couple animals, always the same ones, would work fine, but none of the others. The original antibody that I used I had always done at about 1:100 dilution. I could not get even a 1:25 to work (which is when we decided to try another antibody, which does give me some of the stain at 1:100. And not that I put much stock in it, but 1:100 is also the company's recommended dilution). I have not done any other stains on these tissues, but another tech has used them and had no problems for her stains. Any ideas? Anyone an expert on pten? It's never been one of the finicky stains, but....maybe there's something I'm not thinking of. Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72002 Office (501)364-2785 Fax (501)364-3161 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From gcallis <@t> montana.edu Fri Jun 16 11:21:53 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Jun 16 11:22:01 2006 Subject: Unsubscribing from Re: [Histonet] Who is this MORON? In-Reply-To: References: Message-ID: <6.0.0.22.1.20060616101226.01b7e520@gemini.msu.montana.edu> To prevent kickback messaging to everyone with just a reply to Histonet (a simple reply is for messaging), there are two ways to do this correctly List-Unsubscribe: OR If none of it works, then contact Herb Hagler so this "person" can get off forever! Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 From Terry.Marshall <@t> rothgen.nhs.uk Fri Jun 16 11:30:45 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Fri Jun 16 11:27:51 2006 Subject: [Histonet] Who is this MORON? Message-ID: Hold on Glen. It was a simple request:-) Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Dawson, Glen [mailto:GDawson@dynacaremilwaukee.com] Sent: 16 June 2006 15:59 To: JEFF TATUM ZELIADT; histonet@lists.utsouthwestern.edu Subject: [Histonet] Who is this MORON? If anyone knows who this foul mouthed little kid is or where I can find him please let me know so I can rip his head off and defecate down his neck. In case he has more than 3 or 4 functional brain cells: to unsubscribe, respond to the histonet with the word "unsubscribe" in the subject line & you'll be removed from the list. Save your potty mouth a bit so you can still suck on your nook while you watch Sesame Street. Glen D. Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of JEFF TATUM ZELIADT Sent: Thursday, June 15, 2006 10:53 PM To: sluhisto@yahoo.com; histonet@lists.utsouthwestern.edu Cc: tetriba@slu.edu Subject: RE: [Histonet] Sirius Red Protocol mouse pancreas you are fuckin pissing me off with all these messages. pull you head out of your ass and remove me from your lists ______________________________________________________________ From: Histology SLU To: "histonet@lists.utsouthwestern.edu" CC: tetriba@slu.edu Subject: [Histonet] Sirius Red Protocol mouse pancreas Date: Thu, 15 Jun 2006 09:52:36 -0700 (PDT) >Hello All: > > I have a dilemma that I hope that someone out there can shed some light on. We have an investigator that has been working with mouse FFPE pancreas. We have been doing the processing, embedding, sectioning, and sirius red staining. Lately, however, we have been getting too much red, more than the collagen is staining. The staining pattern is everywhere!! This is a new development and we have tried the following: > > 1. Making new reagents > 2. Evaluating the processes that the mouse went through prior to necropsy. > 3. Did a fixation evaluation to make sure that fixation was not an issue. > 4. Multiple techs performing stain > > We are at a loss to help this investigator and don't want to give up!! We had performed this stain numerous times in the past for this investigator without any problems. > > Any help you can send my way would be appreciated by myself as well as the investigator. > > Susan > > __________________________________________________ >Do You Yahoo!? >Tired of spam? Yahoo! Mail has the best spam protection around >http://mail.yahoo.com >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From specialstainsqueen <@t> hotmail.com Fri Jun 16 11:58:41 2006 From: specialstainsqueen <@t> hotmail.com (Sherri Anderson) Date: Fri Jun 16 11:58:46 2006 Subject: [Histonet] Who is this MORON? In-Reply-To: Message-ID: I had just sent him a direct email stating the same thing that Glen said by the time I read these responses. Of course, to show him a little respect, I addressed him as "Mr. F*** Head". :) >From: "Marshall Terry Dr,Consultant Histopathologist" > >To: "Dawson, Glen" >, >Subject: RE: [Histonet] Who is this MORON? >Date: Fri, 16 Jun 2006 17:30:45 +0100 > >Hold on Glen. It was a simple request:-) > >Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path > Consultant Pathologist > Rotherham General Hospital > South Yorkshire > England > terry.marshall@rothgen.nhs.uk > >-----Original Message----- >From: Dawson, Glen [mailto:GDawson@dynacaremilwaukee.com] >Sent: 16 June 2006 15:59 >To: JEFF TATUM ZELIADT; histonet@lists.utsouthwestern.edu >Subject: [Histonet] Who is this MORON? > > >If anyone knows who this foul mouthed little kid is or where I can find him >please let me know so I can rip his head off and defecate down his neck. > >In case he has more than 3 or 4 functional brain cells: to unsubscribe, >respond to the histonet with the word "unsubscribe" in the subject line & >you'll be removed from the list. Save your potty mouth a bit so you can >still suck on your nook while you watch Sesame Street. > >Glen D. >Milwaukee, WI > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of JEFF >TATUM ZELIADT >Sent: Thursday, June 15, 2006 10:53 PM >To: sluhisto@yahoo.com; histonet@lists.utsouthwestern.edu >Cc: tetriba@slu.edu >Subject: RE: [Histonet] Sirius Red Protocol mouse pancreas > > > > you are fuckin pissing me off with all these messages. pull you head > out of your ass and remove me from your lists > ______________________________________________________________ > > From: Histology SLU > To: "histonet@lists.utsouthwestern.edu" > > CC: tetriba@slu.edu > Subject: [Histonet] Sirius Red Protocol mouse pancreas > Date: Thu, 15 Jun 2006 09:52:36 -0700 (PDT) > >Hello All: > > > > I have a dilemma that I hope that someone out there can shed > some light on. We have an investigator that has been working with > mouse FFPE pancreas. We have been doing the processing, embedding, > sectioning, and sirius red staining. Lately, however, we have been > getting too much red, more than the collagen is staining. The > staining pattern is everywhere!! This is a new development and we > have tried the following: > > > > 1. Making new reagents > > 2. Evaluating the processes that the mouse went through prior > to necropsy. > > 3. Did a fixation evaluation to make sure that fixation was > not an issue. > > 4. Multiple techs performing stain > > > > We are at a loss to help this investigator and don't want to > give up!! We had performed this stain numerous times in the past > for this investigator without any problems. > > > > Any help you can send my way would be appreciated by myself as > well as the investigator. > > > > Susan > > > > __________________________________________________ > >Do You Yahoo!? > >Tired of spam? Yahoo! Mail has the best spam protection around > >http://mail.yahoo.com > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ FREE pop-up blocking with the new MSN Toolbar – get it now! http://toolbar.msn.click-url.com/go/onm00200415ave/direct/01/ From jennifer.l.hofecker <@t> Vanderbilt.Edu Fri Jun 16 12:07:05 2006 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Fri Jun 16 12:09:35 2006 Subject: [Histonet] Who is this MORON? Message-ID: <898D946569A27444B65667A49C074052852D6C@mailbe06.mc.vanderbilt.edu> You know what I find funny.... There were far less "flaming" posts toward this guy than I've had to some of my innocent, honest question/answer posts in the past. Maybe all this time I have been using the wrong "language". Happy Friday everyone! Jennifer From jqb7 <@t> cdc.gov Fri Jun 16 12:12:16 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/NCID/VR)) Date: Fri Jun 16 12:13:14 2006 Subject: [Histonet] Who is this MORON? Message-ID: LOL! The difference is everyone knows this guy is a jerk.............you, obviously, are not! Jeanine -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hofecker, Jennifer L Sent: Friday, June 16, 2006 1:07 PM To: histonet Subject: RE: [Histonet] Who is this MORON? You know what I find funny.... There were far less "flaming" posts toward this guy than I've had to some of my innocent, honest question/answer posts in the past. Maybe all this time I have been using the wrong "language". Happy Friday everyone! Jennifer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From specialstainsqueen <@t> hotmail.com Fri Jun 16 12:13:33 2006 From: specialstainsqueen <@t> hotmail.com (Sherri Anderson) Date: Fri Jun 16 12:13:42 2006 Subject: [Histonet] Who is this MORON? In-Reply-To: Message-ID: Well, I guess I should clarify -- I gave the person the same info as Glen regarding unsubscribing. I made no mention of anything else. :) Sherri >From: "Sherri Anderson" >To: Terry.Marshall@rothgen.nhs.uk, >GDawson@dynacaremilwaukee.com,histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] Who is this MORON? >Date: Fri, 16 Jun 2006 12:58:41 -0400 > >I had just sent him a direct email stating the same thing that Glen said by >the time I read these responses. Of course, to show him a little respect, >I addressed him as "Mr. F*** Head". :) > > >>From: "Marshall Terry Dr,Consultant Histopathologist" >> >>To: "Dawson, Glen" >>, >>Subject: RE: [Histonet] Who is this MORON? >>Date: Fri, 16 Jun 2006 17:30:45 +0100 >> >>Hold on Glen. It was a simple request:-) >> >>Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path >> Consultant Pathologist >> Rotherham General Hospital >> South Yorkshire >> England >> terry.marshall@rothgen.nhs.uk >> >>-----Original Message----- >>From: Dawson, Glen [mailto:GDawson@dynacaremilwaukee.com] >>Sent: 16 June 2006 15:59 >>To: JEFF TATUM ZELIADT; histonet@lists.utsouthwestern.edu >>Subject: [Histonet] Who is this MORON? >> >> >>If anyone knows who this foul mouthed little kid is or where I can find >>him please let me know so I can rip his head off and defecate down his >>neck. >> >>In case he has more than 3 or 4 functional brain cells: to unsubscribe, >>respond to the histonet with the word "unsubscribe" in the subject line & >>you'll be removed from the list. Save your potty mouth a bit so you can >>still suck on your nook while you watch Sesame Street. >> >>Glen D. >>Milwaukee, WI >> >>-----Original Message----- >>From: histonet-bounces@lists.utsouthwestern.edu >>[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of JEFF >>TATUM ZELIADT >>Sent: Thursday, June 15, 2006 10:53 PM >>To: sluhisto@yahoo.com; histonet@lists.utsouthwestern.edu >>Cc: tetriba@slu.edu >>Subject: RE: [Histonet] Sirius Red Protocol mouse pancreas >> >> >> >> you are fuckin pissing me off with all these messages. pull you head >> out of your ass and remove me from your lists >> ______________________________________________________________ >> >> From: Histology SLU >> To: "histonet@lists.utsouthwestern.edu" >> >> CC: tetriba@slu.edu >> Subject: [Histonet] Sirius Red Protocol mouse pancreas >> Date: Thu, 15 Jun 2006 09:52:36 -0700 (PDT) >> >Hello All: >> > >> > I have a dilemma that I hope that someone out there can shed >> some light on. We have an investigator that has been working with >> mouse FFPE pancreas. We have been doing the processing, embedding, >> sectioning, and sirius red staining. Lately, however, we have been >> getting too much red, more than the collagen is staining. The >> staining pattern is everywhere!! This is a new development and we >> have tried the following: >> > >> > 1. Making new reagents >> > 2. Evaluating the processes that the mouse went through prior >> to necropsy. >> > 3. Did a fixation evaluation to make sure that fixation was >> not an issue. >> > 4. Multiple techs performing stain >> > >> > We are at a loss to help this investigator and don't want to >> give up!! We had performed this stain numerous times in the past >> for this investigator without any problems. >> > >> > Any help you can send my way would be appreciated by myself as >> well as the investigator. >> > >> > Susan >> > >> > __________________________________________________ >> >Do You Yahoo!? >> >Tired of spam? Yahoo! Mail has the best spam protection around >> >http://mail.yahoo.com >> >_______________________________________________ >> >Histonet mailing list >> >Histonet@lists.utsouthwestern.edu >> >http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_________________________________________________________________ >FREE pop-up blocking with the new MSN Toolbar – get it now! >http://toolbar.msn.click-url.com/go/onm00200415ave/direct/01/ > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Express yourself instantly with MSN Messenger! Download today - it's FREE! http://messenger.msn.click-url.com/go/onm00200471ave/direct/01/ From mgdelaware <@t> comcast.net Fri Jun 16 12:47:29 2006 From: mgdelaware <@t> comcast.net (Marian Powers) Date: Fri Jun 16 12:47:35 2006 Subject: [Histonet] Shandon TissueWave Message-ID: <001201c6916c$f0767b70$3227c847@D7XQNX91> Anyone using Shandon TissueWave? Any feedback would be appreciated. Thanks, Marian From sbreeden <@t> nmda.nmsu.edu Fri Jun 16 13:02:58 2006 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Jun 16 13:03:06 2006 Subject: [Histonet] WOW!! Message-ID: Now THAT'S a Friday Hour of Fume! I vote for going back to complaining about bottles that drip and people who correct usage of the English language. I think I'll just go cut in some tissues using a very sharp blade and check back in on Monday! Have a weekend, everyone... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87108 From wellborj <@t> mercyhealth.com Fri Jun 16 13:26:57 2006 From: wellborj <@t> mercyhealth.com (Janci Wellborn) Date: Fri Jun 16 13:27:25 2006 Subject: [Histonet] Milestone Med MW tissue processors Message-ID: ** High Priority ** Is anyone using a Microwave Tissue Processor by Milestone Med? We are having a demo on them next week. Any feed back would be appreciated. Thank-You, Janci Wellborn, BS, BSeD, HLT - ASCP Dunes Medical Laboratory 350 West Anchor Drive Dakota Dunes, SD 57049 From funderwood <@t> mcohio.org Fri Jun 16 13:43:40 2006 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Fri Jun 16 13:44:09 2006 Subject: [Histonet] High Profile Micrtome blades Message-ID: I will second that endorsement. Fred >>> "Gladney, Diane C Ms MACH" 6/13/2006 11:42:50 AM >>> C.L. Sturkey makes excellent high and low profile blades. I have been using them for years. Thanks, Diane Diane C. Gladney, HT (ASCP) Supervisor, Histology Dept. Moncrief Army Community Hospital Dept. of Pathology 4500 Stuart St. FT. Jackson, SC 29207 Email: diane.gladney@se.amedd.army.mil Phone: 803-751-2530 FAX: 803-751-7829 DSN: 734-2530 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heather.A.Harper@pcola.med.navy.mil Sent: Tuesday, June 13, 2006 11:22 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] High Profile Micrtome blades I have a Leica Microtome 2235. Currently I am using the Leica 818 high profile blades, and they are terrible. They become dull immediately, slowing down my cutting, because it seems I am changing the blade for every block I cut. Does anyone know of a good high profile blade? I was told the high profile blade by DuraEdge is good. Any feedback would be appreciated. Heather A. Harper _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bwhitaker <@t> brownpathology.com Fri Jun 16 14:09:40 2006 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Fri Jun 16 14:07:36 2006 Subject: [Histonet] comment and question about old blocks Message-ID: <000301c69178$6b49f5b0$3601a8c0@brownpathology.net> Hi All, About the rude and vulgar Histonet subscriber that can't figure out how to unsubscribe, I take some comfort in thinking that someday, a prospective employer will Google that guy, and hopefully will get all of the "colorful" remarks on the histonet to use in his or her decision-making process. Now, on to business: Is there any reason that old (pre-hippa, stuff that is past the CAP requirement for saving blocks) couldn't be given, traded or sold at a small fee (just enough to recover costs) to a research entity? They would not have identification, only a very broad, general diagnosis (from the ancient card file). What does everyone think? Has anyone actually consulted their legal department about this type of issue? Bonnie Whitaker Lab Manager Brown & Associates Medical Laboratories 8076 El Rio Houston, Texas 77054 713-741-6677 From rjbuesa <@t> yahoo.com Fri Jun 16 14:20:09 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 16 14:20:16 2006 Subject: [Histonet] comment and question about old blocks In-Reply-To: <000301c69178$6b49f5b0$3601a8c0@brownpathology.net> Message-ID: <20060616192009.22581.qmail@web61214.mail.yahoo.com> Bonnie: I think you should ask your legal department because, as far as I know, it is forbidden to sell human tissue for any purpose regardless of identification or age. Perhaps you could donate them, but not to sell them (at least that is the regulation in Florida). Again, ask your legal department. Ren? J. Bonnie Whitaker wrote: Hi All, About the rude and vulgar Histonet subscriber that can't figure out how to unsubscribe, I take some comfort in thinking that someday, a prospective employer will Google that guy, and hopefully will get all of the "colorful" remarks on the histonet to use in his or her decision-making process. Now, on to business: Is there any reason that old (pre-hippa, stuff that is past the CAP requirement for saving blocks) couldn't be given, traded or sold at a small fee (just enough to recover costs) to a research entity? They would not have identification, only a very broad, general diagnosis (from the ancient card file). What does everyone think? Has anyone actually consulted their legal department about this type of issue? Bonnie Whitaker Lab Manager Brown & Associates Medical Laboratories 8076 El Rio Houston, Texas 77054 713-741-6677 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ring'em or ping'em. Make PC-to-phone calls as low as 1?/min with Yahoo! Messenger with Voice. From rjbuesa <@t> yahoo.com Fri Jun 16 14:32:47 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 16 14:32:51 2006 Subject: [Histonet] productivity for pathology transcription personnel In-Reply-To: <20060615201907.17095.qmail@web81012.mail.mud.yahoo.com> Message-ID: <20060616193247.10300.qmail@web61217.mail.yahoo.com> Dawn: I have just finished 2 articles (productivity and staffing) and I can offer you the following averages: 1- accessioning data to computer: 120 cases/hour (18 labs). 2- gross transcription: between 13 and 26; average of 24 cases/hour (7 labs). 3- 1 transciptionist every 9,000 cases (34 labs), and 4- i secretary every 17,000 cases (31 labs). If you want I can e-mail you the 2 articles as "pdf" files. Hope this will help you! Ren? J. Dawn Cowie wrote: hello histonetters, I am wondering if any of you have any data on productivity levels/expectations for your transcription people that you are willing to share? What quantity of work performed in an average day? Number of lines transcribed? What kind of lines? Microscopic diagnosis or gross descriptions? Any info will be greatly appreciated. thanks, Dawn Cowie AP Director Bon Secours Health Partners Lab Richmond, VA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. PC-to-Phone calls for ridiculously low rates. From christiegowan <@t> msn.com Fri Jun 16 14:44:46 2006 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Fri Jun 16 14:44:55 2006 Subject: [Histonet] comment and question about old blocks In-Reply-To: <20060616192009.22581.qmail@web61214.mail.yahoo.com> Message-ID: Bonnie and Rene, Rene is correct in saying that human tissue cannot be sold. However it can be licensed for a fee with the patients consent and IRB approval. I'm not sure about the pre-HIPAA tissue though. I work for a tissue repository and we have been licensing human tissue for years. Christie From Tracey.Lenek <@t> CLS.ab.ca Fri Jun 16 16:08:25 2006 From: Tracey.Lenek <@t> CLS.ab.ca (Tracey.Lenek@CLS.ab.ca) Date: Fri Jun 16 16:08:32 2006 Subject: [Histonet] Myo D1 Message-ID: <4BC300747AF87A48BCDF8E48BC2885CE02C1D931@mail1.calgary.com> Hi, We are trying to develop Dako's Myo D1 on the Ventana Benchmark. We are finding alot of cross reactivity with macrophages, endothelial cells, etc. Has anyone had the same experience with this antibody or better yet developed a consistent protocol? Thanks in advance. Tracey CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. From jnocito <@t> satx.rr.com Fri Jun 16 18:32:58 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Jun 16 18:33:07 2006 Subject: [Histonet] Who is this MORON? References: <898D946569A27444B65667A49C074052852D6C@mailbe06.mc.vanderbilt.edu> Message-ID: <002201c6919d$33fbf0c0$0b69ce44@yourxhtr8hvc4p> my turn, my turn (y'all know I live for this kind of thing) Jennifer, people like this moron just say and do things to inflame people on purpose. Unlike people with more than four synapses working, this eye dee ten tee (i d io t) would not understand what an insult is. It could be worse, he (and I use the male pronoun because females never would use such language, in public anyway) could work for you. Then what? There isn't enough Hybiclens made in the world to wash his mouth out. There is only one way to deal with fungus like this. Perform a GMS stain using a microwave or bring his little butt to south Texas, strip him down, tie him down near a fire ant mound, pour honey over him, kick the fire ant mound and watch with glee. OH forget it, that would be a waste of good honey. Just kick the mound. I love Flaming Fridays. Joe "flame me" Nocito ----- Original Message ----- From: "Hofecker, Jennifer L" To: "histonet" Sent: Friday, June 16, 2006 12:07 PM Subject: RE: [Histonet] Who is this MORON? You know what I find funny.... There were far less "flaming" posts toward this guy than I've had to some of my innocent, honest question/answer posts in the past. Maybe all this time I have been using the wrong "language". Happy Friday everyone! Jennifer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.9.0/367 - Release Date: 6/16/2006 From jnocito <@t> satx.rr.com Fri Jun 16 18:38:22 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Jun 16 18:38:29 2006 Subject: [Histonet] comment and question about old blocks References: <000301c69178$6b49f5b0$3601a8c0@brownpathology.net> Message-ID: <002c01c6919d$f49e8400$0b69ce44@yourxhtr8hvc4p> Bonnie, as long as any patient information is not given, it wouldn't be a problem. then again, that's what the VA worker said just before he lost his computer with 2.5 million military names and social security numbers. All CAP says is that if blocks are disposed in a proper manner. Define "proper manner". I see no harm. Joe ----- Original Message ----- From: "Bonnie Whitaker" To: Sent: Friday, June 16, 2006 2:09 PM Subject: [Histonet] comment and question about old blocks > Hi All, > > About the rude and vulgar Histonet subscriber that can't figure out how to > unsubscribe, I take some comfort in thinking that someday, a prospective > employer will Google that guy, and hopefully will get all of the > "colorful" > remarks on the histonet to use in his or her decision-making process. > > Now, on to business: Is there any reason that old (pre-hippa, stuff that > is past the CAP requirement for saving blocks) couldn't be given, traded > or > sold at a small fee (just enough to recover costs) to a research entity? > They would not have identification, only a very broad, general diagnosis > (from the ancient card file). > > What does everyone think? Has anyone actually consulted their legal > department about this type of issue? > > Bonnie Whitaker > Lab Manager > Brown & Associates Medical Laboratories > 8076 El Rio > Houston, Texas 77054 > 713-741-6677 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.394 / Virus Database: 268.9.0/367 - Release Date: 6/16/2006 > > From Marysia33 <@t> hotmail.com Fri Jun 16 22:26:38 2006 From: Marysia33 <@t> hotmail.com (Mary) Date: Fri Jun 16 22:27:17 2006 Subject: [Histonet] Tampa Florida jobs References: <056AFE945F2C1F4292A1F8EBA9B84B5B713555@MI8NYCMAIL15.Mi8.com> Message-ID: I, as several other techs are wondering what jobs may be available in the Tampa Florida area. Please send any info available. Thank you for your time, Marysia From PKamalavenkatesh <@t> wockhardtin.com Sat Jun 17 00:41:19 2006 From: PKamalavenkatesh <@t> wockhardtin.com (PKamalavenkatesh@wockhardtin.com) Date: Sat Jun 17 00:34:30 2006 Subject: [Histonet] reticulocyte counting-flowcytometry-doubts Message-ID: Dear Histonetters, This is Dr.Kamalavenkatesh from wockhardt research center, India. As a part of pre clinical safety evaluation of our NCE'S, we want to standardize the methodology for flowcytometric enumeration of reticulocytes from mouse blood. We are currently attempting to standardize two protocols one using acridine orange and the other using thiazole orange. While doing validation, we are trying to correlate the flowcytometric results to that of manual counting using brilliant methylene blue. But the problem we are currently facing is that we are not able to get the correlation between these two methodologies. Our flow cytometer is one from Beckman Coulter, model FC 500. Any body having any sort of experience in this regard. If you, then can you please share your instrument settings and your protocols. Please excuse me since this topic is somewhat deviated from our regular discussions. Thanks and regards REGARDS Dr.P.Kamalavenkatesh New Drug Discovery- Biology Wockhardt Research Center D-4, MIDC, Chikalthana Aurangabad. Maharastra, India E.mail : Pkamalavenkatesh@wockhardtin.com Please Visit our New Corporate Web Site www.wockhardt.com --------------------- Disclaimer ------------------------------------------------------------------------------ Information transmitted by this E-MAIL is proprietary to Wockhardt Ltd. and/ or its Group Companies and/or,its Customers and is intended for use only by the individual or entity to which it is addressed, and may contain information that is privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. --------------------------------------------------------------------------------------------------------------- From galalmkh <@t> yahoo.com Sat Jun 17 03:38:28 2006 From: galalmkh <@t> yahoo.com (manal galal) Date: Sat Jun 17 03:38:35 2006 Subject: [Histonet] modified gomori stain Message-ID: <20060617083828.36391.qmail@web50211.mail.yahoo.com> Hi, I need help with modified gomori stain. I cant get the muscle to be green. Could anyone help me. Dr. Manal Galal Consultant Pathology Institute of Neuromotor Disabilities Cairo, Egypt histonet-request@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Trichrome staining and fibrin (Marshall Terry Dr, Consultant Histopathologist) 2. substrates (Perry, Margaret) 3. toe nails (Conlon, Paula F.) 4. Re: substrates (Rene J Buesa) 5. Re: toe nails (Rene J Buesa) 6. Re: toe nails (Chris Pomajzl) 7. ScyTek's serum free pro-block protein (Maria Mejia) 8. RE: toe nails (Anthony F. Boris) 9. Manual cover slip method - DiscoverSlip? (Geoffrey White) 10. Re: substrates (Susan Q Wells) 11. Sphingomyelin (mtitford@aol.com) 12. High profile blades (Snider, Deanna) 13. Mechanical testing-Dallas-Fort Worth (Ashwin Nair) ---------------------------------------------------------------------- Message: 1 Date: Wed, 14 Jun 2006 13:39:24 +0100 From: "Marshall Terry Dr, Consultant Histopathologist" Subject: RE: [Histonet] Trichrome staining and fibrin To: "Bryan Hewlett" , Timo V?is?nen , Message-ID: Content-Type: text/plain; charset="iso-8859-1" Well, that's telling me:-) All these years, the success I've had with primary mercury fixation (I had the original article once), and lack of success with anything other was entirely due to something else, the techs washing too much in one instance and little enough in another. Who would have thought it. However it may explain my observation that "At times it works and at times not." Actually, the only time you can be confident that you are looking at fibrin, is when it is in strands - easily recognised in H&E. To call anything else fibrin on any tinctorial stain is something akin to a leap of faith. But - I'll get you back re. your "bees can't fly" thing. Take this/that: :-) The "science has proved that bees can't fly" urban myth originated in a 1934 book by entomologist Antoine Magnan, who discussed a mathematical equation by Andre Sainte-Lague, an engineer. The equation proved that the maximum lift for an aircraft's wings could not be achieved at equivalent speeds of a bee. I.e., an airplane the size of a bee, moving as slowly as a bee, could not fly. Although this did not mean a bee can't fly (which after all does not have stationary wings like the posited teensy aircraft), nevertheless the idea that Magnan's book said bees oughtn't be able to fly began to spread. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Bryan Hewlett [mailto:bhewlett@cogeco.ca] Sent: 13 June 2006 17:14 To: Marshall Terry Dr, Consultant Histopathologist; Timo V?is?nen; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Trichrome staining and fibrin Terry, Actually NOT! True, Lendrum's original papers(1962) recommended fixation in picro-mercuric alcohol for 3 weeks (a tad unnecessary that!). However, both Prof. Lendrum and Bill Slidders also successfully used formaldehyde fixed material that was treated in Bouin immediately prior to staining. The key to all of the Masson Type trichromes on formaldehyde-fixed tissue, is to use a pretreatment in picric acid. This re-aligns the reactive side chains on the proteins, so that there is a predominance of basic amino groups and hence maximal binding of anionic dyes. Mercury fixation is simply not necessary! Nor is Zinc substitution. (see Troubleshooting Histology stains: Horobin and Bancroft 1998, page 129) The lack of consistency, in obtaining good red colour, is due variability in the staining procedure (the post dye water rinses), NOT the fixation. Once this is addressed, the inconsistency goes away. That is true for the original Masson, Picro-Mallory (all variants), MSB and Masson 44/41. Achieving consistent good red colour with formaldehyde fixed material is easy, if the staining mechanisms are understood and those unnecessary water rinses removed. Been doing it for over 40 years! Bryan (Engineers can prove that the bumble bee simply cannot fly. However, the bumble bee doesn't know that and flies anyway) ----- Original Message ----- From: "Marshall Terry Dr, Consultant Histopathologist" To: "Bryan Hewlett" ; "Timo V?is?nen" ; Sent: Tuesday, June 13, 2006 11:27 AM Subject: RE: [Histonet] Trichrome staining and fibrin Bryan, You know full well that MSB required initial staining in mercury. I know that zinc is an adequate replacement. Attempting to get a consistent good red colour with formalin fixed material is futile, as is post-fixation. At times it works and at times not. IMO, Masson is useless for fibrin. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Bryan Hewlett [mailto:bhewlett@cogeco.ca] Sent: 13 June 2006 15:52 To: Timo V?is?nen; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Trichrome staining and fibrin Timo, Try the Lendrum MSB trichrome variant, it was designed to demonstrate fibrin. I will send the method via a separate e-mail. Bryan ----- Original Message ----- From: "Timo V?is?nen" To: Sent: Tuesday, June 13, 2006 10:33 AM Subject: [Histonet] Trichrome staining and fibrin > Hello all, > > I have struggled with getting Masson Trichrome (Goldner) staining to work > with > kidney biopsies. The problem is that our pathologist does not get positive > fibrin staining to glomeruli in diseased kidneys. Those areas of the > glomeruli > that should contain fibrin, at least according to the pathologist, are > green > (Lichtgrun) and not red, as they should be, whatever I do. I have tried to > enhance the staining with Bouin's fixative (+56C) pretreatment but it did > not > change the overall situation. However, the red stain was more intense in > skin > samples containing fibrin (formalin fixed). I have also tried another > Trichrome > staining with Crocein Scarlet 7B without any luck. In our lab kidney > biopsies > are routinely fixed with alcoholic Bouin's. As far as I know it should be > compatible with the Masson protocol we use. Any ideas? Any good protocols > that > I could try? > > Thank's in advance, > > Timo > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Wed, 14 Jun 2006 09:08:36 -0500 From: "Perry, Margaret" Subject: [Histonet] substrates To: Message-ID: Content-Type: text/plain; charset="us-ascii" We are trying to do double staining and are looking for two different colors of substrate and we can't use DAB due to melanin. Does anyone know of a green stain that is xylene compatible? Vector has Nova Red and a blue color but we use hematoxylin as a counterstain. It would mean an extra step for us to counterstain in Methyl Green We use a DAKO autostainer. Margaret Perry HT(ASCP) South Dakota State University Animal Research and Diagnostic Lab Brookings SD 57007 Margaret.Perry@sdstate.edu ------------------------------ Message: 3 Date: Wed, 14 Jun 2006 10:16:10 -0400 From: "Conlon, Paula F." Subject: [Histonet] toe nails To: "Histonet \(E-mail\)" Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi Histonetters, does anyone have a good procedure for sectioning nails and keeping them on the slides during h&e staining and pas staining?Most of the time our sections wash off. Thanks for your help. See our web page at http://www.lahey.org for a full directory of Lahey sites, staff, services and career opportunities. THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS ADDRESSED. IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended recipient, your use of this message for any purpose is strictly prohibited. If you have received this communication in error, please delete the message and notify the sender so that we may correct our records. ------------------------------ Message: 4 Date: Wed, 14 Jun 2006 07:23:19 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] substrates To: "Perry, Margaret" , histonet@lists.utsouthwestern.edu Message-ID: <20060614142319.7044.qmail@web61216.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Margaret: Confronted with that situation I would eliminate the melanine better. Oxidize it with 0.1% potassium permanganate for 2 hours; wash and remove the oxidized melanine with 0.3% oxalic acid followed by washing. The section should be free of melanine pigment/colour afterwards and you can use DAB (for brown) and DAB + Ni salts for dark blue as double substrates. Hope this will help you! Ren? J. "Perry, Margaret" wrote: We are trying to do double staining and are looking for two different colors of substrate and we can't use DAB due to melanin. Does anyone know of a green stain that is xylene compatible? Vector has Nova Red and a blue color but we use hematoxylin as a counterstain. It would mean an extra step for us to counterstain in Methyl Green We use a DAKO autostainer. Margaret Perry HT(ASCP) South Dakota State University Animal Research and Diagnostic Lab Brookings SD 57007 Margaret.Perry@sdstate.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com ------------------------------ Message: 5 Date: Wed, 14 Jun 2006 07:25:13 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] toe nails To: "Conlon, Paula F." , "Histonet \(E-mail\)" Message-ID: <20060614142513.15660.qmail@web61219.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Paula: I am forwarding privately a summary on Toenails I prepared recently that I hope will answer your questions. Ren? J. "Conlon, Paula F." wrote: Hi Histonetters, does anyone have a good procedure for sectioning nails and keeping them on the slides during h&e staining and pas staining?Most of the time our sections wash off. Thanks for your help. See our web page at http://www.lahey.org for a full directory of Lahey sites, staff, services and career opportunities. THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS ADDRESSED. IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended recipient, your use of this message for any purpose is strictly prohibited. If you have received this communication in error, please delete the message and notify the sender so that we may correct our records. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com ------------------------------ Message: 6 Date: Wed, 14 Jun 2006 09:41:48 -0500 From: "Chris Pomajzl" Subject: Re: [Histonet] toe nails To: "HISTONET" Message-ID: <002e01c68fc0$b2fb6330$26fca8c0@CSP> Content-Type: text/plain; charset="iso-8859-1" Good question. We have dealt with this issue for many years, and we have come up with a solution that has worked for some time. First of all, sections are picked up on "+" coated slides that have been smeared with egg albumin. We separate eggs from the grocery store and keep a stock of the egg white albumin in a bottle. Maybe 100ml at a time, keep it refrigerated. When cutting nails, put 2-3 drops of albumin on the slide and smear to cover all of the glass. We cut 3 sections of the nail specimen. 1 for H&E, 1 for PAS, and 1 extra for back-up. We bake the slides at 55'C overnight, and then at 70'C for 20 minutes prior to staining the following day. This seems to work very well for us. ----- Original Message ----- From: "Conlon, Paula F." To: "Histonet (E-mail)" Sent: Wednesday, June 14, 2006 9:16 AM Subject: [Histonet] toe nails Hi Histonetters, does anyone have a good procedure for sectioning nails and keeping them on the slides during h&e staining and pas staining?Most of the time our sections wash off. Thanks for your help. See our web page at http://www.lahey.org for a full directory of Lahey sites, staff, services and career opportunities. THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS ADDRESSED. IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended recipient, your use of this message for any purpose is strictly prohibited. If you have received this communication in error, please delete the message and notify the sender so that we may correct our records. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Wed, 14 Jun 2006 08:11:41 -0700 From: Maria Mejia Subject: [Histonet] ScyTek's serum free pro-block protein To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed To anyone who using ScyTek's serum free pro-block protein, please let me know what you think of it. In IHC, do you (only) use it as a diluent for the antibodies or also use it in the washes? Any information you can provide will be greatly appreciated. Yours Maria Bartola Mejia UCSF Depart. of Neurosurgery San Francisco, CA 94103 ------------------------------ Message: 8 Date: Wed, 14 Jun 2006 11:51:21 -0400 From: "Anthony F. Boris" Subject: RE: [Histonet] toe nails To: "HISTONET" Message-ID: Content-Type: text/plain; charset="utf-8" We use charged slides from Premiere. keep in 65 degree oven for 30 minutes and stain. We have not had any fall off since we switched to these slides. When cutting you can use a 5% solution of KOh for a "surface decal". This breaks down the keratin a little bit (same stuff as in NAIR). Helps to get decent sectioning. Tony -----Original Message----- From: Chris Pomajzl [mailto:cpomajzl@cpllabs.com] Sent: Wed 6/14/2006 10:41 AM To: HISTONET Cc: Subject: Re: [Histonet] toe nails Good question. We have dealt with this issue for many years, and we have come up with a solution that has worked for some time. First of all, sections are picked up on "+" coated slides that have been smeared with egg albumin. We separate eggs from the grocery store and keep a stock of the egg white albumin in a bottle. Maybe 100ml at a time, keep it refrigerated. When cutting nails, put 2-3 drops of albumin on the slide and smear to cover all of the glass. We cut 3 sections of the nail specimen. 1 for H&E, 1 for PAS, and 1 extra for back-up. We bake the slides at 55'C overnight, and then at 70'C for 20 minutes prior to staining the following day. This seems to work very well for us. ----- Original Message ----- From: "Conlon, Paula F." To: "Histonet (E-mail)" Sent: Wednesday, June 14, 2006 9:16 AM Subject: [Histonet] toe nails Hi Histonetters, does anyone have a good procedure for sectioning nails and keeping them on the slides during h&e staining and pas staining?Most of the time our sections wash off. Thanks for your help. See our web page at http://www.lahey.org for a full directory of Lahey sites, staff, services and career opportunities. THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS ADDRESSED. IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended recipient, your use of this message for any purpose is strictly prohibited. If you have received this communication in error, please delete the message and notify the sender so that we may correct our records. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Wed, 14 Jun 2006 08:52:10 -0700 (PDT) From: Geoffrey White Subject: [Histonet] Manual cover slip method - DiscoverSlip? To: histonet@lists.utsouthwestern.edu Message-ID: <20060614155210.68428.qmail@web36513.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Dear all, at the time our research group is looking for new manual method for cover our slides during the hybridization. In the past we used standard glass cover slips but we are not really satisfied with this method. Just I saw at Biocompare a new method with the name DiscoverSlip. This technology looks very interesting. Have any work with DiscoverSlip or saw this technology working? Thanks Geoffrey __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com ------------------------------ Message: 10 Date: Wed, 14 Jun 2006 12:01:42 -0400 From: Susan Q Wells Subject: Re: [Histonet] substrates To: Rene J Buesa Cc: histonet@lists.utsouthwestern.edu, "Perry, Margaret" Message-ID: <449032E6.8030300@bms.com> Content-Type: text/plain; format=flowed; charset=ISO-8859-1 You may lose your epitopes with this procedure, if so 10% H202 in PBS for 30 minutes may lighten the melanin pigment enough to view your chromagen. I recently used Vulcan Fast Red and Bajoran Purple from Biocare Medical with great success where melanin pigment was present. Sue Wells HT(ASCP), QIHC Rene J Buesa wrote: >Margaret: > Confronted with that situation I would eliminate the melanine better. Oxidize it with 0.1% potassium permanganate for 2 hours; wash and remove the oxidized melanine with 0.3% oxalic acid followed by washing. The section should be free of melanine pigment/colour afterwards and you can use DAB (for brown) and DAB + Ni salts for dark blue as double substrates. === message truncated === --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. From mstahl <@t> bvha.org Sat Jun 17 09:37:07 2006 From: mstahl <@t> bvha.org (Stahl, Michael) Date: Sat Jun 17 09:36:43 2006 Subject: [Histonet] Who is this MORON? Message-ID: <4C878E714B21EB4F8EB159777B8822EE5B6826@bvfyms01.net.bvha.org> Why stick the poor fire ants with this creature?? Michael Stahl HT (ASCP) BVRHC Findlay, OH 45840 From valleygal <@t> aol.com Sat Jun 17 10:13:30 2006 From: valleygal <@t> aol.com (valleygal@aol.com) Date: Sat Jun 17 10:13:43 2006 Subject: [Histonet] Who is this MORON? In-Reply-To: <4C878E714B21EB4F8EB159777B8822EE5B6826@bvfyms01.net.bvha.org> References: <4C878E714B21EB4F8EB159777B8822EE5B6826@bvfyms01.net.bvha.org> Message-ID: <8C86037B9B0E1F2-114C-330B@MBLK-R03.sysops.aol.com> Because fire ants do a REALLY good job! I wonder how many control blocks we could make out of this moron??? Andi -----Original Message----- From: Stahl, Michael To: histonet@lists.utsouthwestern.edu Sent: Sat, 17 Jun 2006 10:37:07 -0400 Subject: Re: [Histonet] Who is this MORON? Why stick the poor fire ants with this creature?? Michael Stahl HT (ASCP) BVRHC Findlay, OH 45840 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Check out AOL.com today. Breaking news, video search, pictures, email and IM. All on demand. Always Free. From katri <@t> cogeco.ca Sat Jun 17 12:52:15 2006 From: katri <@t> cogeco.ca (Katri Tuomala) Date: Sat Jun 17 12:52:47 2006 Subject: [Histonet] modified gomori stain References: <20060617083828.36391.qmail@web50211.mail.yahoo.com> Message-ID: <007901c69236$d46f21a0$6a9a9618@Katri> If you are referring to Gomori's one step trichrome stain, muscle should be staining red, not green as collagen. See web page http://stainsfile.info/StainsFile/theory/tri_gen.htm Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "manal galal" To: Sent: Saturday, June 17, 2006 4:38 AM Subject: [Histonet] modified gomori stain > Hi, > I need help with modified gomori stain. I cant get the muscle to be > green. Could anyone help me. > Dr. Manal > Galal > Consultant Pathology > Institute of > Neuromotor Disabilities > Cairo, Egypt > > histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: Trichrome staining and fibrin > (Marshall Terry Dr, Consultant Histopathologist) > 2. substrates (Perry, Margaret) > 3. toe nails (Conlon, Paula F.) > 4. Re: substrates (Rene J Buesa) > 5. Re: toe nails (Rene J Buesa) > 6. Re: toe nails (Chris Pomajzl) > 7. ScyTek's serum free pro-block protein (Maria Mejia) > 8. RE: toe nails (Anthony F. Boris) > 9. Manual cover slip method - DiscoverSlip? (Geoffrey White) > 10. Re: substrates (Susan Q Wells) > 11. Sphingomyelin (mtitford@aol.com) > 12. High profile blades (Snider, Deanna) > 13. Mechanical testing-Dallas-Fort Worth (Ashwin Nair) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 14 Jun 2006 13:39:24 +0100 > From: "Marshall Terry Dr, Consultant Histopathologist" > > Subject: RE: [Histonet] Trichrome staining and fibrin > To: "Bryan Hewlett" , Timo V?is?nen > , > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > Well, that's telling me:-) > > All these years, the success I've had with primary mercury fixation (I had > the original article once), and lack of success with anything other was > entirely due to something else, the techs washing too much in one instance > and little enough in another. > Who would have thought it. > However it may explain my observation that "At times it works and at times > not." > > Actually, the only time you can be confident that you are looking at > fibrin, is when it is in strands - easily recognised in H&E. > To call anything else fibrin on any tinctorial stain is something akin to > a leap of faith. > > But - I'll get you back re. your "bees can't fly" thing. > > Take this/that: :-) > > The "science has proved that bees can't fly" urban myth originated in a > 1934 book by entomologist Antoine Magnan, who discussed a mathematical > equation by Andre Sainte-Lague, an engineer. The equation proved that the > maximum lift for an aircraft's wings could not be achieved at equivalent > speeds of a bee. I.e., an airplane the size of a bee, moving as slowly as > a bee, could not fly. Although this did not mean a bee can't fly (which > after all does not have stationary wings like the posited teensy > aircraft), > nevertheless the idea that Magnan's book said bees oughtn't be able to fly > began to spread. > > > Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path > Consultant Pathologist > Rotherham General Hospital > South Yorkshire > England > terry.marshall@rothgen.nhs.uk > > -----Original Message----- > From: Bryan Hewlett [mailto:bhewlett@cogeco.ca] > Sent: 13 June 2006 17:14 > To: Marshall Terry Dr, Consultant Histopathologist; Timo V?is?nen; > histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Trichrome staining and fibrin > > > Terry, > > Actually NOT! > True, Lendrum's original papers(1962) recommended fixation in > picro-mercuric > alcohol for 3 weeks (a tad unnecessary that!). > However, both Prof. Lendrum and Bill Slidders also successfully used > formaldehyde fixed material that was treated in Bouin immediately prior to > staining. > The key to all of the Masson Type trichromes on formaldehyde-fixed tissue, > is to use a pretreatment in picric acid. > This re-aligns the reactive side chains on the proteins, so that there is > a > predominance of basic amino groups and hence maximal binding of anionic > dyes. > Mercury fixation is simply not necessary! Nor is Zinc substitution. (see > Troubleshooting Histology stains: Horobin and Bancroft 1998, page 129) > The lack of consistency, in obtaining good red colour, is due variability > in > the staining procedure (the post dye water rinses), NOT the fixation. > Once this is addressed, the inconsistency goes away. > That is true for the original Masson, Picro-Mallory (all variants), MSB > and > Masson 44/41. > Achieving consistent good red colour with formaldehyde fixed material is > easy, if the staining mechanisms are understood and those unnecessary > water > rinses removed. > Been doing it for over 40 years! > > Bryan > (Engineers can prove that the bumble bee simply cannot fly. However, the > bumble bee doesn't know that and flies anyway) > > ----- Original Message ----- > From: "Marshall Terry Dr, Consultant Histopathologist" > > To: "Bryan Hewlett" ; "Timo V?is?nen" > ; > Sent: Tuesday, June 13, 2006 11:27 AM > Subject: RE: [Histonet] Trichrome staining and fibrin > > > Bryan, > > You know full well that MSB required initial staining in mercury. I know > that zinc is an adequate replacement. > Attempting to get a consistent good red colour with formalin fixed > material > is futile, as is post-fixation. > At times it works and at times not. > > IMO, Masson is useless for fibrin. > > Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path > Consultant Pathologist > Rotherham General Hospital > South Yorkshire > England > terry.marshall@rothgen.nhs.uk > > -----Original Message----- > From: Bryan Hewlett [mailto:bhewlett@cogeco.ca] > Sent: 13 June 2006 15:52 > To: Timo V?is?nen; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Trichrome staining and fibrin > > > Timo, > > Try the Lendrum MSB trichrome variant, it was designed to demonstrate > fibrin. > I will send the method via a separate e-mail. > > Bryan > > ----- Original Message ----- > From: "Timo V?is?nen" > To: > Sent: Tuesday, June 13, 2006 10:33 AM > Subject: [Histonet] Trichrome staining and fibrin > > >> Hello all, >> >> I have struggled with getting Masson Trichrome (Goldner) staining to work >> with >> kidney biopsies. The problem is that our pathologist does not get >> positive >> fibrin staining to glomeruli in diseased kidneys. Those areas of the >> glomeruli >> that should contain fibrin, at least according to the pathologist, are >> green >> (Lichtgrun) and not red, as they should be, whatever I do. I have tried >> to >> enhance the staining with Bouin's fixative (+56C) pretreatment but it did >> not >> change the overall situation. However, the red stain was more intense in >> skin >> samples containing fibrin (formalin fixed). I have also tried another >> Trichrome >> staining with Crocein Scarlet 7B without any luck. In our lab kidney >> biopsies >> are routinely fixed with alcoholic Bouin's. As far as I know it should be >> compatible with the Masson protocol we use. Any ideas? Any good protocols >> that >> I could try? >> >> Thank's in advance, >> >> Timo >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 2 > Date: Wed, 14 Jun 2006 09:08:36 -0500 > From: "Perry, Margaret" > Subject: [Histonet] substrates > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > We are trying to do double staining and are looking for two different > colors of substrate and we can't use DAB due to melanin. Does anyone > know of a green stain that is xylene compatible? Vector has Nova Red > and a blue color but we use hematoxylin as a counterstain. It would > mean an extra step for us to counterstain in Methyl Green > > We use a DAKO autostainer. > > > > Margaret Perry HT(ASCP) > > South Dakota State University > > Animal Research and Diagnostic Lab > > Brookings SD 57007 > > Margaret.Perry@sdstate.edu > > > > > > ------------------------------ > > Message: 3 > Date: Wed, 14 Jun 2006 10:16:10 -0400 > From: "Conlon, Paula F." > > Subject: [Histonet] toe nails > To: "Histonet \(E-mail\)" > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > Hi Histonetters, does anyone have a good procedure for sectioning nails > and keeping them on the slides during h&e staining and pas staining?Most > of the time our sections wash off. Thanks for your help. > > > See our web page at http://www.lahey.org for a full directory of Lahey > sites, staff, services and career opportunities. > > THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS > ADDRESSED. IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND > EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended > recipient, your use of this message for any purpose is strictly > prohibited. If you have received this communication in error, please > delete the message and notify the sender so that we may correct our > records. > > > ------------------------------ > > Message: 4 > Date: Wed, 14 Jun 2006 07:23:19 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] substrates > To: "Perry, Margaret" , > histonet@lists.utsouthwestern.edu > Message-ID: <20060614142319.7044.qmail@web61216.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Margaret: > Confronted with that situation I would eliminate the melanine better. > Oxidize it with 0.1% potassium permanganate for 2 hours; wash and remove > the oxidized melanine with 0.3% oxalic acid followed by washing. The > section should be free of melanine pigment/colour afterwards and you can > use DAB (for brown) and DAB + Ni salts for dark blue as double substrates. > Hope this will help you! > Ren? J. > > "Perry, Margaret" wrote: > We are trying to do double staining and are looking for two different > colors of substrate and we can't use DAB due to melanin. Does anyone > know of a green stain that is xylene compatible? Vector has Nova Red > and a blue color but we use hematoxylin as a counterstain. It would > mean an extra step for us to counterstain in Methyl Green > > We use a DAKO autostainer. > > > > Margaret Perry HT(ASCP) > > South Dakota State University > > Animal Research and Diagnostic Lab > > Brookings SD 57007 > > Margaret.Perry@sdstate.edu > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > ------------------------------ > > Message: 5 > Date: Wed, 14 Jun 2006 07:25:13 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] toe nails > To: "Conlon, Paula F." > , "Histonet > \(E-mail\)" > Message-ID: <20060614142513.15660.qmail@web61219.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Paula: > I am forwarding privately a summary on Toenails I prepared recently that I > hope will answer your questions. > Ren? J. > > "Conlon, Paula F." > wrote: > Hi Histonetters, does anyone have a good procedure for sectioning nails > and keeping them on the slides during h&e staining and pas staining?Most > of the time our sections wash off. Thanks for your help. > > > See our web page at http://www.lahey.org for a full directory of Lahey > sites, staff, services and career opportunities. > > THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS > ADDRESSED. IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND > EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended > recipient, your use of this message for any purpose is strictly > prohibited. If you have received this communication in error, please > delete the message and notify the sender so that we may correct our > records. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > ------------------------------ > > Message: 6 > Date: Wed, 14 Jun 2006 09:41:48 -0500 > From: "Chris Pomajzl" > Subject: Re: [Histonet] toe nails > To: "HISTONET" > Message-ID: <002e01c68fc0$b2fb6330$26fca8c0@CSP> > Content-Type: text/plain; charset="iso-8859-1" > > Good question. We have dealt with this issue for many years, and we have > come up with a solution that has worked for some time. > > First of all, sections are picked up on "+" coated slides that have been > smeared with egg albumin. We separate eggs from the grocery store and keep > a > stock of the egg white albumin in a bottle. Maybe 100ml at a time, keep it > refrigerated. When cutting nails, put 2-3 drops of albumin on the slide > and > smear to cover all of the glass. > > We cut 3 sections of the nail specimen. 1 for H&E, 1 for PAS, and 1 extra > for back-up. We bake the slides at 55'C overnight, and then at 70'C for 20 > minutes prior to staining the following day. > > This seems to work very well for us. > > ----- Original Message ----- > From: "Conlon, Paula F." > > To: "Histonet (E-mail)" > Sent: Wednesday, June 14, 2006 9:16 AM > Subject: [Histonet] toe nails > > > Hi Histonetters, does anyone have a good procedure for sectioning nails > and > keeping them on the slides during h&e staining and pas staining?Most of > the > time our sections wash off. Thanks for your help. > > > See our web page at http://www.lahey.org for a full directory of Lahey > sites, staff, services and career opportunities. > > THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS > ADDRESSED. > IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT > FROM > DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended recipient, > your > use of this message for any purpose is strictly prohibited. If you have > received this communication in error, please delete the message and notify > the sender so that we may correct our records. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 7 > Date: Wed, 14 Jun 2006 08:11:41 -0700 > From: Maria Mejia > Subject: [Histonet] ScyTek's serum free pro-block protein > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed > > To anyone who using ScyTek's serum free pro-block protein, please let > me know > what you think of it. In IHC, do you (only) use it as a diluent for > the antibodies or also > use it in the washes? Any information you can provide will be greatly > appreciated. > > Yours > > Maria Bartola Mejia > UCSF > Depart. of Neurosurgery > San Francisco, CA 94103 > > > > ------------------------------ > > Message: 8 > Date: Wed, 14 Jun 2006 11:51:21 -0400 > From: "Anthony F. Boris" > Subject: RE: [Histonet] toe nails > To: "HISTONET" > Message-ID: > Content-Type: text/plain; charset="utf-8" > > We use charged slides from Premiere. keep in 65 degree oven for 30 minutes > and stain. We have not had any fall off since we switched to these slides. > When cutting you can use a 5% solution of KOh for a "surface decal". This > breaks down the keratin a little bit (same stuff as in NAIR). Helps to get > decent sectioning. > > Tony > > -----Original Message----- > From: Chris Pomajzl [mailto:cpomajzl@cpllabs.com] > Sent: Wed 6/14/2006 10:41 AM > To: HISTONET > Cc: > Subject: Re: [Histonet] toe nails > > > > Good question. We have dealt with this issue for many years, and we have > come up with a solution that has worked for some time. > > First of all, sections are picked up on "+" coated slides that have been > smeared with egg albumin. We separate eggs from the grocery store and keep > a > stock of the egg white albumin in a bottle. Maybe 100ml at a time, keep it > refrigerated. When cutting nails, put 2-3 drops of albumin on the slide > and > smear to cover all of the glass. > > We cut 3 sections of the nail specimen. 1 for H&E, 1 for PAS, and 1 extra > for back-up. We bake the slides at 55'C overnight, and then at 70'C for 20 > minutes prior to staining the following day. > > This seems to work very well for us. > > ----- Original Message ----- > From: "Conlon, Paula F." > > To: "Histonet (E-mail)" > Sent: Wednesday, June 14, 2006 9:16 AM > Subject: [Histonet] toe nails > > > Hi Histonetters, does anyone have a good procedure for sectioning nails > and > keeping them on the slides during h&e staining and pas staining?Most of > the > time our sections wash off. Thanks for your help. > > > See our web page at http://www.lahey.org for a full directory of Lahey > sites, staff, services and career opportunities. > > THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS > ADDRESSED. > IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT > FROM > DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended recipient, > your > use of this message for any purpose is strictly prohibited. If you have > received this communication in error, please delete the message and notify > the sender so that we may correct our records. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 9 > Date: Wed, 14 Jun 2006 08:52:10 -0700 (PDT) > From: Geoffrey White > Subject: [Histonet] Manual cover slip method - DiscoverSlip? > To: histonet@lists.utsouthwestern.edu > Message-ID: <20060614155210.68428.qmail@web36513.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > > Dear all, > > > > at the time our research group is looking for new manual method for cover > our slides during the hybridization. In the past we used standard glass > cover slips but we are not really satisfied with this method. > > Just I saw at Biocompare a new method with the name DiscoverSlip. This > technology looks very interesting. > > Have any work with DiscoverSlip or saw this technology working? > > > > Thanks > > Geoffrey > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > ------------------------------ > > Message: 10 > Date: Wed, 14 Jun 2006 12:01:42 -0400 > From: Susan Q Wells > Subject: Re: [Histonet] substrates > To: Rene J Buesa > Cc: histonet@lists.utsouthwestern.edu, "Perry, Margaret" > > Message-ID: <449032E6.8030300@bms.com> > Content-Type: text/plain; format=flowed; charset=ISO-8859-1 > > You may lose your epitopes with this procedure, if so 10% H202 in PBS > for 30 minutes may lighten the melanin pigment enough > to view your chromagen. I recently used Vulcan Fast Red and Bajoran > Purple from Biocare Medical with great success where melanin pigment was > present. > > Sue Wells HT(ASCP), QIHC > > Rene J Buesa wrote: > >>Margaret: >> Confronted with that situation I would eliminate the melanine better. >> Oxidize it with 0.1% potassium permanganate for 2 hours; wash and remove >> the oxidized melanine with 0.3% oxalic acid followed by washing. The >> section should be free of melanine pigment/colour afterwards and you can >> use DAB (for brown) and DAB + Ni salts for dark blue as double >> substrates. > > === message truncated === > > > --------------------------------- > Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ > countries) for 2?/min or less. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vanann702 <@t> skmc.gov.ae Sat Jun 17 22:47:35 2006 From: vanann702 <@t> skmc.gov.ae (Anne Van Binsbergen) Date: Sat Jun 17 22:44:56 2006 Subject: [Histonet] modified gomori stain Message-ID: In a frozen section, muscle should be green - how else would one demonstrate ragged red fibres??? Anne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Katri Tuomala Sent: Saturday, June 17, 2006 9:52 PM To: manal galal; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] modified gomori stain If you are referring to Gomori's one step trichrome stain, muscle should be staining red, not green as collagen. See web page http://stainsfile.info/StainsFile/theory/tri_gen.htm Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "manal galal" To: Sent: Saturday, June 17, 2006 4:38 AM Subject: [Histonet] modified gomori stain > Hi, > I need help with modified gomori stain. I cant get the muscle to be > green. Could anyone help me. > Dr. Manal > Galal > Consultant Pathology > Institute of > Neuromotor Disabilities > Cairo, Egypt > > histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: Trichrome staining and fibrin > (Marshall Terry Dr, Consultant Histopathologist) > 2. substrates (Perry, Margaret) > 3. toe nails (Conlon, Paula F.) > 4. Re: substrates (Rene J Buesa) > 5. Re: toe nails (Rene J Buesa) > 6. Re: toe nails (Chris Pomajzl) > 7. ScyTek's serum free pro-block protein (Maria Mejia) > 8. RE: toe nails (Anthony F. Boris) > 9. Manual cover slip method - DiscoverSlip? (Geoffrey White) > 10. Re: substrates (Susan Q Wells) > 11. Sphingomyelin (mtitford@aol.com) > 12. High profile blades (Snider, Deanna) > 13. Mechanical testing-Dallas-Fort Worth (Ashwin Nair) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 14 Jun 2006 13:39:24 +0100 > From: "Marshall Terry Dr, Consultant Histopathologist" > > Subject: RE: [Histonet] Trichrome staining and fibrin > To: "Bryan Hewlett" , Timo V?is?nen > , > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > Well, that's telling me:-) > > All these years, the success I've had with primary mercury fixation (I had > the original article once), and lack of success with anything other was > entirely due to something else, the techs washing too much in one instance > and little enough in another. > Who would have thought it. > However it may explain my observation that "At times it works and at times > not." > > Actually, the only time you can be confident that you are looking at > fibrin, is when it is in strands - easily recognised in H&E. > To call anything else fibrin on any tinctorial stain is something akin to > a leap of faith. > > But - I'll get you back re. your "bees can't fly" thing. > > Take this/that: :-) > > The "science has proved that bees can't fly" urban myth originated in a > 1934 book by entomologist Antoine Magnan, who discussed a mathematical > equation by Andre Sainte-Lague, an engineer. The equation proved that the > maximum lift for an aircraft's wings could not be achieved at equivalent > speeds of a bee. I.e., an airplane the size of a bee, moving as slowly as > a bee, could not fly. Although this did not mean a bee can't fly (which > after all does not have stationary wings like the posited teensy > aircraft), > nevertheless the idea that Magnan's book said bees oughtn't be able to fly > began to spread. > > > Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path > Consultant Pathologist > Rotherham General Hospital > South Yorkshire > England > terry.marshall@rothgen.nhs.uk > > -----Original Message----- > From: Bryan Hewlett [mailto:bhewlett@cogeco.ca] > Sent: 13 June 2006 17:14 > To: Marshall Terry Dr, Consultant Histopathologist; Timo V?is?nen; > histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Trichrome staining and fibrin > > > Terry, > > Actually NOT! > True, Lendrum's original papers(1962) recommended fixation in > picro-mercuric > alcohol for 3 weeks (a tad unnecessary that!). > However, both Prof. Lendrum and Bill Slidders also successfully used > formaldehyde fixed material that was treated in Bouin immediately prior to > staining. > The key to all of the Masson Type trichromes on formaldehyde-fixed tissue, > is to use a pretreatment in picric acid. > This re-aligns the reactive side chains on the proteins, so that there is > a > predominance of basic amino groups and hence maximal binding of anionic > dyes. > Mercury fixation is simply not necessary! Nor is Zinc substitution. (see > Troubleshooting Histology stains: Horobin and Bancroft 1998, page 129) > The lack of consistency, in obtaining good red colour, is due variability > in > the staining procedure (the post dye water rinses), NOT the fixation. > Once this is addressed, the inconsistency goes away. > That is true for the original Masson, Picro-Mallory (all variants), MSB > and > Masson 44/41. > Achieving consistent good red colour with formaldehyde fixed material is > easy, if the staining mechanisms are understood and those unnecessary > water > rinses removed. > Been doing it for over 40 years! > > Bryan > (Engineers can prove that the bumble bee simply cannot fly. However, the > bumble bee doesn't know that and flies anyway) > > ----- Original Message ----- > From: "Marshall Terry Dr, Consultant Histopathologist" > > To: "Bryan Hewlett" ; "Timo V?is?nen" > ; > Sent: Tuesday, June 13, 2006 11:27 AM > Subject: RE: [Histonet] Trichrome staining and fibrin > > > Bryan, > > You know full well that MSB required initial staining in mercury. I know > that zinc is an adequate replacement. > Attempting to get a consistent good red colour with formalin fixed > material > is futile, as is post-fixation. > At times it works and at times not. > > IMO, Masson is useless for fibrin. > > Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path > Consultant Pathologist > Rotherham General Hospital > South Yorkshire > England > terry.marshall@rothgen.nhs.uk > > -----Original Message----- > From: Bryan Hewlett [mailto:bhewlett@cogeco.ca] > Sent: 13 June 2006 15:52 > To: Timo V?is?nen; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Trichrome staining and fibrin > > > Timo, > > Try the Lendrum MSB trichrome variant, it was designed to demonstrate > fibrin. > I will send the method via a separate e-mail. > > Bryan > > ----- Original Message ----- > From: "Timo V?is?nen" > To: > Sent: Tuesday, June 13, 2006 10:33 AM > Subject: [Histonet] Trichrome staining and fibrin > > >> Hello all, >> >> I have struggled with getting Masson Trichrome (Goldner) staining to work >> with >> kidney biopsies. The problem is that our pathologist does not get >> positive >> fibrin staining to glomeruli in diseased kidneys. Those areas of the >> glomeruli >> that should contain fibrin, at least according to the pathologist, are >> green >> (Lichtgrun) and not red, as they should be, whatever I do. I have tried >> to >> enhance the staining with Bouin's fixative (+56C) pretreatment but it did >> not >> change the overall situation. However, the red stain was more intense in >> skin >> samples containing fibrin (formalin fixed). I have also tried another >> Trichrome >> staining with Crocein Scarlet 7B without any luck. In our lab kidney >> biopsies >> are routinely fixed with alcoholic Bouin's. As far as I know it should be >> compatible with the Masson protocol we use. Any ideas? Any good protocols >> that >> I could try? >> >> Thank's in advance, >> >> Timo >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 2 > Date: Wed, 14 Jun 2006 09:08:36 -0500 > From: "Perry, Margaret" > Subject: [Histonet] substrates > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > We are trying to do double staining and are looking for two different > colors of substrate and we can't use DAB due to melanin. Does anyone > know of a green stain that is xylene compatible? Vector has Nova Red > and a blue color but we use hematoxylin as a counterstain. It would > mean an extra step for us to counterstain in Methyl Green > > We use a DAKO autostainer. > > > > Margaret Perry HT(ASCP) > > South Dakota State University > > Animal Research and Diagnostic Lab > > Brookings SD 57007 > > Margaret.Perry@sdstate.edu > > > > > > ------------------------------ > > Message: 3 > Date: Wed, 14 Jun 2006 10:16:10 -0400 > From: "Conlon, Paula F." > > Subject: [Histonet] toe nails > To: "Histonet \(E-mail\)" > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > Hi Histonetters, does anyone have a good procedure for sectioning nails > and keeping them on the slides during h&e staining and pas staining?Most > of the time our sections wash off. Thanks for your help. > > > See our web page at http://www.lahey.org for a full directory of Lahey > sites, staff, services and career opportunities. > > THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS > ADDRESSED. IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND > EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended > recipient, your use of this message for any purpose is strictly > prohibited. If you have received this communication in error, please > delete the message and notify the sender so that we may correct our > records. > > > ------------------------------ > > Message: 4 > Date: Wed, 14 Jun 2006 07:23:19 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] substrates > To: "Perry, Margaret" , > histonet@lists.utsouthwestern.edu > Message-ID: <20060614142319.7044.qmail@web61216.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Margaret: > Confronted with that situation I would eliminate the melanine better. > Oxidize it with 0.1% potassium permanganate for 2 hours; wash and remove > the oxidized melanine with 0.3% oxalic acid followed by washing. The > section should be free of melanine pigment/colour afterwards and you can > use DAB (for brown) and DAB + Ni salts for dark blue as double substrates. > Hope this will help you! > Ren? J. > > "Perry, Margaret" wrote: > We are trying to do double staining and are looking for two different > colors of substrate and we can't use DAB due to melanin. Does anyone > know of a green stain that is xylene compatible? Vector has Nova Red > and a blue color but we use hematoxylin as a counterstain. It would > mean an extra step for us to counterstain in Methyl Green > > We use a DAKO autostainer. > > > > Margaret Perry HT(ASCP) > > South Dakota State University > > Animal Research and Diagnostic Lab > > Brookings SD 57007 > > Margaret.Perry@sdstate.edu > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > ------------------------------ > > Message: 5 > Date: Wed, 14 Jun 2006 07:25:13 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] toe nails > To: "Conlon, Paula F." > , "Histonet > \(E-mail\)" > Message-ID: <20060614142513.15660.qmail@web61219.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Paula: > I am forwarding privately a summary on Toenails I prepared recently that I > hope will answer your questions. > Ren? J. > > "Conlon, Paula F." > wrote: > Hi Histonetters, does anyone have a good procedure for sectioning nails > and keeping them on the slides during h&e staining and pas staining?Most > of the time our sections wash off. Thanks for your help. > > > See our web page at http://www.lahey.org for a full directory of Lahey > sites, staff, services and career opportunities. > > THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS > ADDRESSED. IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND > EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended > recipient, your use of this message for any purpose is strictly > prohibited. If you have received this communication in error, please > delete the message and notify the sender so that we may correct our > records. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > ------------------------------ > > Message: 6 > Date: Wed, 14 Jun 2006 09:41:48 -0500 > From: "Chris Pomajzl" > Subject: Re: [Histonet] toe nails > To: "HISTONET" > Message-ID: <002e01c68fc0$b2fb6330$26fca8c0@CSP> > Content-Type: text/plain; charset="iso-8859-1" > > Good question. We have dealt with this issue for many years, and we have > come up with a solution that has worked for some time. > > First of all, sections are picked up on "+" coated slides that have been > smeared with egg albumin. We separate eggs from the grocery store and keep > a > stock of the egg white albumin in a bottle. Maybe 100ml at a time, keep it > refrigerated. When cutting nails, put 2-3 drops of albumin on the slide > and > smear to cover all of the glass. > > We cut 3 sections of the nail specimen. 1 for H&E, 1 for PAS, and 1 extra > for back-up. We bake the slides at 55'C overnight, and then at 70'C for 20 > minutes prior to staining the following day. > > This seems to work very well for us. > > ----- Original Message ----- > From: "Conlon, Paula F." > > To: "Histonet (E-mail)" > Sent: Wednesday, June 14, 2006 9:16 AM > Subject: [Histonet] toe nails > > > Hi Histonetters, does anyone have a good procedure for sectioning nails > and > keeping them on the slides during h&e staining and pas staining?Most of > the > time our sections wash off. Thanks for your help. > > > See our web page at http://www.lahey.org for a full directory of Lahey > sites, staff, services and career opportunities. > > THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS > ADDRESSED. > IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT > FROM > DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended recipient, > your > use of this message for any purpose is strictly prohibited. If you have > received this communication in error, please delete the message and notify > the sender so that we may correct our records. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 7 > Date: Wed, 14 Jun 2006 08:11:41 -0700 > From: Maria Mejia > Subject: [Histonet] ScyTek's serum free pro-block protein > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed > > To anyone who using ScyTek's serum free pro-block protein, please let > me know > what you think of it. In IHC, do you (only) use it as a diluent for > the antibodies or also > use it in the washes? Any information you can provide will be greatly > appreciated. > > Yours > > Maria Bartola Mejia > UCSF > Depart. of Neurosurgery > San Francisco, CA 94103 > > > > ------------------------------ > > Message: 8 > Date: Wed, 14 Jun 2006 11:51:21 -0400 > From: "Anthony F. Boris" > Subject: RE: [Histonet] toe nails > To: "HISTONET" > Message-ID: > Content-Type: text/plain; charset="utf-8" > > We use charged slides from Premiere. keep in 65 degree oven for 30 minutes > and stain. We have not had any fall off since we switched to these slides. > When cutting you can use a 5% solution of KOh for a "surface decal". This > breaks down the keratin a little bit (same stuff as in NAIR). Helps to get > decent sectioning. > > Tony > > -----Original Message----- > From: Chris Pomajzl [mailto:cpomajzl@cpllabs.com] > Sent: Wed 6/14/2006 10:41 AM > To: HISTONET > Cc: > Subject: Re: [Histonet] toe nails > > > > Good question. We have dealt with this issue for many years, and we have > come up with a solution that has worked for some time. > > First of all, sections are picked up on "+" coated slides that have been > smeared with egg albumin. We separate eggs from the grocery store and keep > a > stock of the egg white albumin in a bottle. Maybe 100ml at a time, keep it > refrigerated. When cutting nails, put 2-3 drops of albumin on the slide > and > smear to cover all of the glass. > > We cut 3 sections of the nail specimen. 1 for H&E, 1 for PAS, and 1 extra > for back-up. We bake the slides at 55'C overnight, and then at 70'C for 20 > minutes prior to staining the following day. > > This seems to work very well for us. > > ----- Original Message ----- > From: "Conlon, Paula F." > > To: "Histonet (E-mail)" > Sent: Wednesday, June 14, 2006 9:16 AM > Subject: [Histonet] toe nails > > > Hi Histonetters, does anyone have a good procedure for sectioning nails > and > keeping them on the slides during h&e staining and pas staining?Most of > the > time our sections wash off. Thanks for your help. > > > See our web page at http://www.lahey.org for a full directory of Lahey > sites, staff, services and career opportunities. > > THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS > ADDRESSED. > IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT > FROM > DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended recipient, > your > use of this message for any purpose is strictly prohibited. If you have > received this communication in error, please delete the message and notify > the sender so that we may correct our records. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 9 > Date: Wed, 14 Jun 2006 08:52:10 -0700 (PDT) > From: Geoffrey White > Subject: [Histonet] Manual cover slip method - DiscoverSlip? > To: histonet@lists.utsouthwestern.edu > Message-ID: <20060614155210.68428.qmail@web36513.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > > Dear all, > > > > at the time our research group is looking for new manual method for cover > our slides during the hybridization. In the past we used standard glass > cover slips but we are not really satisfied with this method. > > Just I saw at Biocompare a new method with the name DiscoverSlip. This > technology looks very interesting. > > Have any work with DiscoverSlip or saw this technology working? > > > > Thanks > > Geoffrey > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > ------------------------------ > > Message: 10 > Date: Wed, 14 Jun 2006 12:01:42 -0400 > From: Susan Q Wells > Subject: Re: [Histonet] substrates > To: Rene J Buesa > Cc: histonet@lists.utsouthwestern.edu, "Perry, Margaret" > > Message-ID: <449032E6.8030300@bms.com> > Content-Type: text/plain; format=flowed; charset=ISO-8859-1 > > You may lose your epitopes with this procedure, if so 10% H202 in PBS > for 30 minutes may lighten the melanin pigment enough > to view your chromagen. I recently used Vulcan Fast Red and Bajoran > Purple from Biocare Medical with great success where melanin pigment was > present. > > Sue Wells HT(ASCP), QIHC > > Rene J Buesa wrote: > >>Margaret: >> Confronted with that situation I would eliminate the melanine better. >> Oxidize it with 0.1% potassium permanganate for 2 hours; wash and remove >> the oxidized melanine with 0.3% oxalic acid followed by washing. The >> section should be free of melanine pigment/colour afterwards and you can >> use DAB (for brown) and DAB + Ni salts for dark blue as double >> substrates. > > === message truncated === > > > --------------------------------- > Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ > countries) for 2?/min or less. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo <@t> f2s.com Sun Jun 18 03:28:11 2006 From: kemlo <@t> f2s.com (kemlo) Date: Sun Jun 18 03:28:17 2006 Subject: [Histonet] Who is this MORON? In-Reply-To: Message-ID: <20060618082808.E8D0C6C20FC@outmail.freedom2surf.net> Maybe he could have phrased it a tad more diplomatically but he does have a valid point; albeit anatomically I think he is asking too much. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marshall Terry Dr,Consultant Histopathologist Sent: 16 June 2006 17:31 To: Dawson, Glen; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Who is this MORON? Hold on Glen. It was a simple request:-) Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Dawson, Glen [mailto:GDawson@dynacaremilwaukee.com] Sent: 16 June 2006 15:59 To: JEFF TATUM ZELIADT; histonet@lists.utsouthwestern.edu Subject: [Histonet] Who is this MORON? If anyone knows who this foul mouthed little kid is or where I can find him please let me know so I can rip his head off and defecate down his neck. In case he has more than 3 or 4 functional brain cells: to unsubscribe, respond to the histonet with the word "unsubscribe" in the subject line & you'll be removed from the list. Save your potty mouth a bit so you can still suck on your nook while you watch Sesame Street. Glen D. Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of JEFF TATUM ZELIADT Sent: Thursday, June 15, 2006 10:53 PM To: sluhisto@yahoo.com; histonet@lists.utsouthwestern.edu Cc: tetriba@slu.edu Subject: RE: [Histonet] Sirius Red Protocol mouse pancreas you are fuckin pissing me off with all these messages. pull you head out of your ass and remove me from your lists ______________________________________________________________ From: Histology SLU To: "histonet@lists.utsouthwestern.edu" CC: tetriba@slu.edu Subject: [Histonet] Sirius Red Protocol mouse pancreas Date: Thu, 15 Jun 2006 09:52:36 -0700 (PDT) >Hello All: > > I have a dilemma that I hope that someone out there can shed some light on. We have an investigator that has been working with mouse FFPE pancreas. We have been doing the processing, embedding, sectioning, and sirius red staining. Lately, however, we have been getting too much red, more than the collagen is staining. The staining pattern is everywhere!! This is a new development and we have tried the following: > > 1. Making new reagents > 2. Evaluating the processes that the mouse went through prior to necropsy. > 3. Did a fixation evaluation to make sure that fixation was not an issue. > 4. Multiple techs performing stain > > We are at a loss to help this investigator and don't want to give up!! We had performed this stain numerous times in the past for this investigator without any problems. > > Any help you can send my way would be appreciated by myself as well as the investigator. > > Susan > > __________________________________________________ >Do You Yahoo!? >Tired of spam? Yahoo! Mail has the best spam protection around >http://mail.yahoo.com >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jxccny <@t> yahoo.com Sun Jun 18 08:19:37 2006 From: jxccny <@t> yahoo.com (x j) Date: Sun Jun 18 08:19:44 2006 Subject: [Histonet] modified gomori stain In-Reply-To: <007901c69236$d46f21a0$6a9a9618@Katri> Message-ID: <20060618131937.24742.qmail@web51406.mail.yahoo.com> How to withdraw from Hisonet? Katri Tuomala wrote: If you are referring to Gomori's one step trichrome stain, muscle should be staining red, not green as collagen. See web page http://stainsfile.info/StainsFile/theory/tri_gen.htm Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "manal galal" To: Sent: Saturday, June 17, 2006 4:38 AM Subject: [Histonet] modified gomori stain > Hi, > I need help with modified gomori stain. I cant get the muscle to be > green. Could anyone help me. > Dr. Manal > Galal > Consultant Pathology > Institute of > Neuromotor Disabilities > Cairo, Egypt > > histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: Trichrome staining and fibrin > (Marshall Terry Dr, Consultant Histopathologist) > 2. substrates (Perry, Margaret) > 3. toe nails (Conlon, Paula F.) > 4. Re: substrates (Rene J Buesa) > 5. Re: toe nails (Rene J Buesa) > 6. Re: toe nails (Chris Pomajzl) > 7. ScyTek's serum free pro-block protein (Maria Mejia) > 8. RE: toe nails (Anthony F. Boris) > 9. Manual cover slip method - DiscoverSlip? (Geoffrey White) > 10. Re: substrates (Susan Q Wells) > 11. Sphingomyelin (mtitford@aol.com) > 12. High profile blades (Snider, Deanna) > 13. Mechanical testing-Dallas-Fort Worth (Ashwin Nair) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 14 Jun 2006 13:39:24 +0100 > From: "Marshall Terry Dr, Consultant Histopathologist" > > Subject: RE: [Histonet] Trichrome staining and fibrin > To: "Bryan Hewlett" , Timo V?is?nen > , > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > Well, that's telling me:-) > > All these years, the success I've had with primary mercury fixation (I had > the original article once), and lack of success with anything other was > entirely due to something else, the techs washing too much in one instance > and little enough in another. > Who would have thought it. > However it may explain my observation that "At times it works and at times > not." > > Actually, the only time you can be confident that you are looking at > fibrin, is when it is in strands - easily recognised in H&E. > To call anything else fibrin on any tinctorial stain is something akin to > a leap of faith. > > But - I'll get you back re. your "bees can't fly" thing. > > Take this/that: :-) > > The "science has proved that bees can't fly" urban myth originated in a > 1934 book by entomologist Antoine Magnan, who discussed a mathematical > equation by Andre Sainte-Lague, an engineer. The equation proved that the > maximum lift for an aircraft's wings could not be achieved at equivalent > speeds of a bee. I.e., an airplane the size of a bee, moving as slowly as > a bee, could not fly. Although this did not mean a bee can't fly (which > after all does not have stationary wings like the posited teensy > aircraft), > nevertheless the idea that Magnan's book said bees oughtn't be able to fly > began to spread. > > > Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path > Consultant Pathologist > Rotherham General Hospital > South Yorkshire > England > terry.marshall@rothgen.nhs.uk > > -----Original Message----- > From: Bryan Hewlett [mailto:bhewlett@cogeco.ca] > Sent: 13 June 2006 17:14 > To: Marshall Terry Dr, Consultant Histopathologist; Timo V?is?nen; > histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Trichrome staining and fibrin > > > Terry, > > Actually NOT! > True, Lendrum's original papers(1962) recommended fixation in > picro-mercuric > alcohol for 3 weeks (a tad unnecessary that!). > However, both Prof. Lendrum and Bill Slidders also successfully used > formaldehyde fixed material that was treated in Bouin immediately prior to > staining. > The key to all of the Masson Type trichromes on formaldehyde-fixed tissue, > is to use a pretreatment in picric acid. > This re-aligns the reactive side chains on the proteins, so that there is > a > predominance of basic amino groups and hence maximal binding of anionic > dyes. > Mercury fixation is simply not necessary! Nor is Zinc substitution. (see > Troubleshooting Histology stains: Horobin and Bancroft 1998, page 129) > The lack of consistency, in obtaining good red colour, is due variability > in > the staining procedure (the post dye water rinses), NOT the fixation. > Once this is addressed, the inconsistency goes away. > That is true for the original Masson, Picro-Mallory (all variants), MSB > and > Masson 44/41. > Achieving consistent good red colour with formaldehyde fixed material is > easy, if the staining mechanisms are understood and those unnecessary > water > rinses removed. > Been doing it for over 40 years! > > Bryan > (Engineers can prove that the bumble bee simply cannot fly. However, the > bumble bee doesn't know that and flies anyway) > > ----- Original Message ----- > From: "Marshall Terry Dr, Consultant Histopathologist" > > To: "Bryan Hewlett" ; "Timo V?is?nen" > ; > Sent: Tuesday, June 13, 2006 11:27 AM > Subject: RE: [Histonet] Trichrome staining and fibrin > > > Bryan, > > You know full well that MSB required initial staining in mercury. I know > that zinc is an adequate replacement. > Attempting to get a consistent good red colour with formalin fixed > material > is futile, as is post-fixation. > At times it works and at times not. > > IMO, Masson is useless for fibrin. > > Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path > Consultant Pathologist > Rotherham General Hospital > South Yorkshire > England > terry.marshall@rothgen.nhs.uk > > -----Original Message----- > From: Bryan Hewlett [mailto:bhewlett@cogeco.ca] > Sent: 13 June 2006 15:52 > To: Timo V?is?nen; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Trichrome staining and fibrin > > > Timo, > > Try the Lendrum MSB trichrome variant, it was designed to demonstrate > fibrin. > I will send the method via a separate e-mail. > > Bryan > > ----- Original Message ----- > From: "Timo V?is?nen" > To: > Sent: Tuesday, June 13, 2006 10:33 AM > Subject: [Histonet] Trichrome staining and fibrin > > >> Hello all, >> >> I have struggled with getting Masson Trichrome (Goldner) staining to work >> with >> kidney biopsies. The problem is that our pathologist does not get >> positive >> fibrin staining to glomeruli in diseased kidneys. Those areas of the >> glomeruli >> that should contain fibrin, at least according to the pathologist, are >> green >> (Lichtgrun) and not red, as they should be, whatever I do. I have tried >> to >> enhance the staining with Bouin's fixative (+56C) pretreatment but it did >> not >> change the overall situation. However, the red stain was more intense in >> skin >> samples containing fibrin (formalin fixed). I have also tried another >> Trichrome >> staining with Crocein Scarlet 7B without any luck. In our lab kidney >> biopsies >> are routinely fixed with alcoholic Bouin's. As far as I know it should be >> compatible with the Masson protocol we use. Any ideas? Any good protocols >> that >> I could try? >> >> Thank's in advance, >> >> Timo >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 2 > Date: Wed, 14 Jun 2006 09:08:36 -0500 > From: "Perry, Margaret" > Subject: [Histonet] substrates > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > We are trying to do double staining and are looking for two different > colors of substrate and we can't use DAB due to melanin. Does anyone > know of a green stain that is xylene compatible? Vector has Nova Red > and a blue color but we use hematoxylin as a counterstain. It would > mean an extra step for us to counterstain in Methyl Green > > We use a DAKO autostainer. > > > > Margaret Perry HT(ASCP) > > South Dakota State University > > Animal Research and Diagnostic Lab > > Brookings SD 57007 > > Margaret.Perry@sdstate.edu > > > > > > ------------------------------ > > Message: 3 > Date: Wed, 14 Jun 2006 10:16:10 -0400 > From: "Conlon, Paula F." > > Subject: [Histonet] toe nails > To: "Histonet \(E-mail\)" > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > Hi Histonetters, does anyone have a good procedure for sectioning nails > and keeping them on the slides during h&e staining and pas staining?Most > of the time our sections wash off. Thanks for your help. > > > See our web page at http://www.lahey.org for a full directory of Lahey > sites, staff, services and career opportunities. > > THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS > ADDRESSED. IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND > EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended > recipient, your use of this message for any purpose is strictly > prohibited. If you have received this communication in error, please > delete the message and notify the sender so that we may correct our > records. > > > ------------------------------ > > Message: 4 > Date: Wed, 14 Jun 2006 07:23:19 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] substrates > To: "Perry, Margaret" , > histonet@lists.utsouthwestern.edu > Message-ID: <20060614142319.7044.qmail@web61216.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Margaret: > Confronted with that situation I would eliminate the melanine better. > Oxidize it with 0.1% potassium permanganate for 2 hours; wash and remove > the oxidized melanine with 0.3% oxalic acid followed by washing. The > section should be free of melanine pigment/colour afterwards and you can > use DAB (for brown) and DAB + Ni salts for dark blue as double substrates. > Hope this will help you! > Ren?J. > > "Perry, Margaret" wrote: > We are trying to do double staining and are looking for two different > colors of substrate and we can't use DAB due to melanin. Does anyone > know of a green stain that is xylene compatible? Vector has Nova Red > and a blue color but we use hematoxylin as a counterstain. It would > mean an extra step for us to counterstain in Methyl Green > > We use a DAKO autostainer. > > > > Margaret Perry HT(ASCP) > > South Dakota State University > > Animal Research and Diagnostic Lab > > Brookings SD 57007 > > Margaret.Perry@sdstate.edu > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > ------------------------------ > > Message: 5 > Date: Wed, 14 Jun 2006 07:25:13 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] toe nails > To: "Conlon, Paula F." > , "Histonet > \(E-mail\)" > Message-ID: <20060614142513.15660.qmail@web61219.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Paula: > I am forwarding privately a summary on Toenails I prepared recently that I > hope will answer your questions. > Ren?J. > > "Conlon, Paula F." > wrote: > Hi Histonetters, does anyone have a good procedure for sectioning nails > and keeping them on the slides during h&e staining and pas staining?Most > of the time our sections wash off. Thanks for your help. > > > See our web page at http://www.lahey.org for a full directory of Lahey > sites, staff, services and career opportunities. > > THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS > ADDRESSED. IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND > EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended > recipient, your use of this message for any purpose is strictly > prohibited. If you have received this communication in error, please > delete the message and notify the sender so that we may correct our > records. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > ------------------------------ > > Message: 6 > Date: Wed, 14 Jun 2006 09:41:48 -0500 > From: "Chris Pomajzl" > Subject: Re: [Histonet] toe nails > To: "HISTONET" > Message-ID: <002e01c68fc0$b2fb6330$26fca8c0@CSP> > Content-Type: text/plain; charset="iso-8859-1" > > Good question. We have dealt with this issue for many years, and we have > come up with a solution that has worked for some time. > > First of all, sections are picked up on "+" coated slides that have been > smeared with egg albumin. We separate eggs from the grocery store and keep > a > stock of the egg white albumin in a bottle. Maybe 100ml at a time, keep it > refrigerated. When cutting nails, put 2-3 drops of albumin on the slide > and > smear to cover all of the glass. > > We cut 3 sections of the nail specimen. 1 for H&E, 1 for PAS, and 1 extra > for back-up. We bake the slides at 55'C overnight, and then at 70'C for 20 > minutes prior to staining the following day. > > This seems to work very well for us. > > ----- Original Message ----- > From: "Conlon, Paula F." > > To: "Histonet (E-mail)" > Sent: Wednesday, June 14, 2006 9:16 AM > Subject: [Histonet] toe nails > > > Hi Histonetters, does anyone have a good procedure for sectioning nails > and > keeping them on the slides during h&e staining and pas staining?Most of > the > time our sections wash off. Thanks for your help. > > > See our web page at http://www.lahey.org for a full directory of Lahey > sites, staff, services and career opportunities. > > THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS > ADDRESSED. > IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT > FROM > DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended recipient, > your > use of this message for any purpose is strictly prohibited. If you have > received this communication in error, please delete the message and notify > the sender so that we may correct our records. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 7 > Date: Wed, 14 Jun 2006 08:11:41 -0700 > From: Maria Mejia > Subject: [Histonet] ScyTek's serum free pro-block protein > To: histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed > > To anyone who using ScyTek's serum free pro-block protein, please let > me know > what you think of it. In IHC, do you (only) use it as a diluent for > the antibodies or also > use it in the washes? Any information you can provide will be greatly > appreciated. > > Yours > > Maria Bartola Mejia > UCSF > Depart. of Neurosurgery > San Francisco, CA 94103 > > > > ------------------------------ > > Message: 8 > Date: Wed, 14 Jun 2006 11:51:21 -0400 > From: "Anthony F. Boris" > Subject: RE: [Histonet] toe nails > To: "HISTONET" > Message-ID: > Content-Type: text/plain; charset="utf-8" > > We use charged slides from Premiere. keep in 65 degree oven for 30 minutes > and stain. We have not had any fall off since we switched to these slides. > When cutting you can use a 5% solution of KOh for a "surface decal". This > breaks down the keratin a little bit (same stuff as in NAIR). Helps to get > decent sectioning. > > Tony > > -----Original Message----- > From: Chris Pomajzl [mailto:cpomajzl@cpllabs.com] > Sent: Wed 6/14/2006 10:41 AM > To: HISTONET > Cc: > Subject: Re: [Histonet] toe nails > > > > Good question. We have dealt with this issue for many years, and we have > come up with a solution that has worked for some time. > > First of all, sections are picked up on "+" coated slides that have been > smeared with egg albumin. We separate eggs from the grocery store and keep > a > stock of the egg white albumin in a bottle. Maybe 100ml at a time, keep it > refrigerated. When cutting nails, put 2-3 drops of albumin on the slide > and > smear to cover all of the glass. > > We cut 3 sections of the nail specimen. 1 for H&E, 1 for PAS, and 1 extra > for back-up. We bake the slides at 55'C overnight, and then at 70'C for 20 > minutes prior to staining the following day. > > This seems to work very well for us. > > ----- Original Message ----- > From: "Conlon, Paula F." > > To: "Histonet (E-mail)" > Sent: Wednesday, June 14, 2006 9:16 AM > Subject: [Histonet] toe nails > > > Hi Histonetters, does anyone have a good procedure for sectioning nails > and > keeping them on the slides during h&e staining and pas staining?Most of > the > time our sections wash off. Thanks for your help. > > > See our web page at http://www.lahey.org for a full directory of Lahey > sites, staff, services and career opportunities. > > THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS > ADDRESSED. > IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND EXEMPT > FROM > DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended recipient, > your > use of this message for any purpose is strictly prohibited. If you have > received this communication in error, please delete the message and notify > the sender so that we may correct our records. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 9 > Date: Wed, 14 Jun 2006 08:52:10 -0700 (PDT) > From: Geoffrey White > Subject: [Histonet] Manual cover slip method - DiscoverSlip? > To: histonet@lists.utsouthwestern.edu > Message-ID: <20060614155210.68428.qmail@web36513.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > > Dear all, > > > > at the time our research group is looking for new manual method for cover > our slides during the hybridization. In the past we used standard glass > cover slips but we are not really satisfied with this method. > > Just I saw at Biocompare a new method with the name DiscoverSlip. This > technology looks very interesting. > > Have any work with DiscoverSlip or saw this technology working? > > > > Thanks > > Geoffrey > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > ------------------------------ > > Message: 10 > Date: Wed, 14 Jun 2006 12:01:42 -0400 > From: Susan Q Wells > Subject: Re: [Histonet] substrates > To: Rene J Buesa > Cc: histonet@lists.utsouthwestern.edu, "Perry, Margaret" > > Message-ID: <449032E6.8030300@bms.com> > Content-Type: text/plain; format=flowed; charset=ISO-8859-1 > > You may lose your epitopes with this procedure, if so 10% H202 in PBS > for 30 minutes may lighten the melanin pigment enough > to view your chromagen. I recently used Vulcan Fast Red and Bajoran === message truncated === --------------------------------- Do you Yahoo!? Next-gen email? Have it all with the all-new Yahoo! Mail Beta. From LRaff <@t> lab.uropartners.com Sun Jun 18 09:30:07 2006 From: LRaff <@t> lab.uropartners.com (Lester Raff) Date: Sun Jun 18 09:30:10 2006 Subject: [Histonet] Recruiting part time histologist Message-ID: <5DA1CA5D0B98A84985B545A24423B822A4F3@UPLAB01.uplab.local> Immediate openning! Our friendly, private lab in west Chicago suburbs is recruiting a part time histologist for day shift. If you know of anyone who might be interested, please give us a call! Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory ph: 708-486-0076 fax: 708-486-0080 From asmith <@t> mail.barry.edu Sun Jun 18 11:38:35 2006 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Sun Jun 18 11:38:41 2006 Subject: [Histonet] comment and question about old blocks In-Reply-To: <002c01c6919d$f49e8400$0b69ce44@yourxhtr8hvc4p> Message-ID: <5D2189E74151CC42BEC02906BA8996322B91FD@exchsrv01.barrynet.barry.edu> Many academic histochemists, myself included, do almost of our work on mouse tissue, simply because it is so hard to get human tissue. The availability of human tissue, both normal and pathological, would be a great blessing to academic research. For some of us, who are fed up with the poor quality of commercial slides, it would advance our teaching as well. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Friday, June 16, 2006 7:38 PM To: Bonnie Whitaker; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] comment and question about old blocks Bonnie, as long as any patient information is not given, it wouldn't be a problem. then again, that's what the VA worker said just before he lost his computer with 2.5 million military names and social security numbers. All CAP says is that if blocks are disposed in a proper manner. Define "proper manner". I see no harm. Joe ----- Original Message ----- From: "Bonnie Whitaker" To: Sent: Friday, June 16, 2006 2:09 PM Subject: [Histonet] comment and question about old blocks > Hi All, > > About the rude and vulgar Histonet subscriber that can't figure out how to > unsubscribe, I take some comfort in thinking that someday, a prospective > employer will Google that guy, and hopefully will get all of the > "colorful" > remarks on the histonet to use in his or her decision-making process. > > Now, on to business: Is there any reason that old (pre-hippa, stuff that > is past the CAP requirement for saving blocks) couldn't be given, traded > or > sold at a small fee (just enough to recover costs) to a research entity? > They would not have identification, only a very broad, general diagnosis > (from the ancient card file). > > What does everyone think? Has anyone actually consulted their legal > department about this type of issue? > > Bonnie Whitaker > Lab Manager > Brown & Associates Medical Laboratories > 8076 El Rio > Houston, Texas 77054 > 713-741-6677 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.394 / Virus Database: 268.9.0/367 - Release Date: 6/16/2006 > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From jstaruk <@t> masshistology.com Sun Jun 18 12:14:49 2006 From: jstaruk <@t> masshistology.com (Jim Staruk) Date: Sun Jun 18 12:16:56 2006 Subject: [Histonet] Two questions on anti-mouse fibroblast antibody In-Reply-To: <5D2189E74151CC42BEC02906BA8996322B91FD@exchsrv01.barrynet.barry.edu> Message-ID: <000101c692fa$b4d2f000$6500a8c0@FrontOffice> Is anyone using Biogenesis's rat anti-mouse fibroblast antibody (Cat. # 4462-1007, clone ER-TR7)? They claim it works on paraffin sections, they say they sell lots of this antibody and never had a complaint but I can't get it to work on FFPE mouse skin. I've tried just about every pre-treatment. Can anyone recommend an antibody to use for labeling fibroblasts in mouse skin on FFPE sections? Thanks a bunch. Jim ______________________ Jim Staruk Mass Histology Service www.masshistology.com From bwhitaker <@t> brownpathology.com Sun Jun 18 22:01:25 2006 From: bwhitaker <@t> brownpathology.com (bwhitaker@brownpathology.com) Date: Sun Jun 18 21:01:33 2006 Subject: [Histonet] modified gomori stain Message-ID: <200606182101.aa13119@MessagEX.iapc.net> Katri, I think that the muscle they were referring to is the modified Gomori's that the neuropath people use. The normal muscle is green when it is done correctly (and they are always looking for 'ragged red fibers'). I have the protocol somewhere... I'll look for it tomorrow when I'm back in the lab. Bonnie Whitaker Brown & Associates Medical Laboratories Houston, TX Katri Tuomala wrote : > If you are referring to Gomori's one step trichrome stain, muscle should = > be=20 > staining red, not green as collagen. See web page=20 > http://stainsfile.info/StainsFile/theory/tri_gen.htm > > Katri > > Katri Tuomala > Hamilton, Ontario, Canada > > ----- Original Message -----=20 > From: "manal galal" <galalmkh@yahoo.com> > To: <histonet@lists.utsouthwestern.edu> > Sent: Saturday, June 17, 2006 4:38 AM > Subject: [Histonet] modified gomori stain > > > > Hi, > > I need help with modified gomori stain. I cant get the muscle to > be= > =20 > > green. Could anyone help me. > > Dr. > Man= > al=20 > > Galal > > Consultant Pathology > > > Institute= > of=20 > > Neuromotor Disabilities > > Cairo, > Egyp= > t > > > > histonet-request@lists.utsouthwestern.edu > wrote: > > Send Histonet mailing list submissions to > > histonet@lists.utsouthwestern.edu > > > > To subscribe or unsubscribe via the World Wide Web, visit > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > or, via email, send a message with subject or body 'help' to > > histonet-request@lists.utsouthwestern.edu > > > > You can reach the person managing the list at > > histonet-owner@lists.utsouthwestern.edu > > > > When replying, please edit your Subject line so it is more specific > > than "Re: Contents of Histonet digest..." > > > > > > Today's Topics: > > > > 1. RE: Trichrome staining and fibrin > > (Marshall Terry Dr, Consultant Histopathologist) > > 2. substrates (Perry, Margaret) > > 3. toe nails (Conlon, Paula F.) > > 4. Re: substrates (Rene J Buesa) > > 5. Re: toe nails (Rene J Buesa) > > 6. Re: toe nails (Chris Pomajzl) > > 7. ScyTek's serum free pro-block protein (Maria Mejia) > > 8. RE: toe nails (Anthony F. Boris) > > 9. Manual cover slip method - DiscoverSlip? (Geoffrey White) > > 10. Re: substrates (Susan Q Wells) > > 11. Sphingomyelin (mtitford@aol.com) > > 12. High profile blades (Snider, Deanna) > > 13. Mechanical testing-Dallas-Fort Worth (Ashwin Nair) > > > > > > ---------------------------------------------------------------------- > > > > Message: 1 > > Date: Wed, 14 Jun 2006 13:39:24 +0100 > > From: "Marshall Terry Dr, Consultant Histopathologist" > > > > Subject: RE: [Histonet] Trichrome staining and fibrin > > To: "Bryan Hewlett" , Timo V?is?nen > > , > > Message-ID: > > > > Content-Type: text/plain; charset=3D"iso-8859-1" > > > > Well, that's telling me:-) > > > > All these years, the success I've had with primary mercury fixation (I > = > had=20 > > the original article once), and lack of success with anything other > was= > =20 > > entirely due to something else, the techs washing too much in one > insta= > nce=20 > > and little enough in another. > > Who would have thought it. > > However it may explain my observation that "At times it works and at > ti= > mes=20 > > not." > > > > Actually, the only time you can be confident that you are looking at=20 > > fibrin, is when it is in strands - easily recognised in H&E. > > To call anything else fibrin on any tinctorial stain is something akin > = > to=20 > > a leap of faith. > > > > But - I'll get you back re. your "bees can't fly" thing. > > > > Take this/that: :-) > > > > The "science has proved that bees can't fly" urban myth > originated in a= > =20 > > 1934 book by entomologist Antoine Magnan, who discussed a > mathematical=20 > > equation by Andre Sainte-Lague, an engineer. The equation proved that > t= > he=20 > > maximum lift for an aircraft's wings could not be achieved at > equivalen= > t=20 > > speeds of a bee. I.e., an airplane the size of a bee, moving as slowly > = > as=20 > > a bee, could not fly. Although this did not mean a bee can't fly > (which= > =20 > > after all does not have stationary wings like the posited teensy=20 > > aircraft), > > nevertheless the idea that Magnan's book said bees oughtn't be able to > = > fly=20 > > began to spread. > > > > > > Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path > > Consultant Pathologist > > Rotherham General Hospital > > South Yorkshire > > England > > terry.marshall@rothgen.nhs.uk > > > > -----Original Message----- > > From: Bryan Hewlett [mailto:bhewlett@cogeco.ca] > > Sent: 13 June 2006 17:14 > > To: Marshall Terry Dr, Consultant Histopathologist; Timo V=E4is=E4nen; > > histonet@lists.utsouthwestern.edu > > Subject: Re: [Histonet] Trichrome staining and fibrin > > > > > > Terry, > > > > Actually NOT! > > True, Lendrum's original papers(1962) recommended fixation in=20 > > picro-mercuric > > alcohol for 3 weeks (a tad unnecessary that!). > > However, both Prof. Lendrum and Bill Slidders also successfully used > > formaldehyde fixed material that was treated in Bouin immediately > prior= > to > > staining. > > The key to all of the Masson Type trichromes on formaldehyde-fixed > tiss= > ue, > > is to use a pretreatment in picric acid. > > This re-aligns the reactive side chains on the proteins, so that there > = > is=20 > > a > > predominance of basic amino groups and hence maximal binding of anionic > > dyes. > > Mercury fixation is simply not necessary! Nor is Zinc substitution. > (se= > e > > Troubleshooting Histology stains: Horobin and Bancroft 1998, page 129) > > The lack of consistency, in obtaining good red colour, is due > variabili= > ty=20 > > in > > the staining procedure (the post dye water rinses), NOT the fixation. > > Once this is addressed, the inconsistency goes away. > > That is true for the original Masson, Picro-Mallory (all variants), > MSB= > =20 > > and > > Masson 44/41. > > Achieving consistent good red colour with formaldehyde fixed material > i= > s > > easy, if the staining mechanisms are understood and those > unnecessary=20 > > water > > rinses removed. > > Been doing it for over 40 years! > > > > Bryan > > (Engineers can prove that the bumble bee simply cannot fly. However, > th= > e > > bumble bee doesn't know that and flies anyway) > > > > ----- Original Message -----=20 > > From: "Marshall Terry Dr, Consultant Histopathologist" > > > > To: "Bryan Hewlett" ; "Timo V=E4is=E4nen" > > ; > > Sent: Tuesday, June 13, 2006 11:27 AM > > Subject: RE: [Histonet] Trichrome staining and fibrin > > > > > > Bryan, > > > > You know full well that MSB required initial staining in mercury. I > kno= > w > > that zinc is an adequate replacement. > > Attempting to get a consistent good red colour with formalin fixed=20 > > material > > is futile, as is post-fixation. > > At times it works and at times not. > > > > IMO, Masson is useless for fibrin. > > > > Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path > > Consultant Pathologist > > Rotherham General Hospital > > South Yorkshire > > England > > terry.marshall@rothgen.nhs.uk > > > > -----Original Message----- > > From: Bryan Hewlett [mailto:bhewlett@cogeco.ca] > > Sent: 13 June 2006 15:52 > > To: Timo V=E4is=E4nen; histonet@lists.utsouthwestern.edu > > Subject: Re: [Histonet] Trichrome staining and fibrin > > > > > > Timo, > > > > Try the Lendrum MSB trichrome variant, it was designed to demonstrate > > fibrin. > > I will send the method via a separate e-mail. > > > > Bryan > > > > ----- Original Message -----=20 > > From: "Timo V=E4is=E4nen" > > To: > > Sent: Tuesday, June 13, 2006 10:33 AM > > Subject: [Histonet] Trichrome staining and fibrin > > > > > >> Hello all, > >> > >> I have struggled with getting Masson Trichrome (Goldner) staining to > w= > ork > >> with > >> kidney biopsies. The problem is that our pathologist does not > get=20 > >> positive > >> fibrin staining to glomeruli in diseased kidneys. Those areas of > the > >> glomeruli > >> that should contain fibrin, at least according to the pathologist, > are > >> green > >> (Lichtgrun) and not red, as they should be, whatever I do. I have > trie= > d=20 > >> to > >> enhance the staining with Bouin's fixative (+56C) pretreatment but it > = > did > >> not > >> change the overall situation. However, the red stain was more intense > = > in > >> skin > >> samples containing fibrin (formalin fixed). I have also tried > another > >> Trichrome > >> staining with Crocein Scarlet 7B without any luck. In our lab > kidney > >> biopsies > >> are routinely fixed with alcoholic Bouin's. As far as I know it > should= > be > >> compatible with the Masson protocol we use. Any ideas? Any good > protoc= > ols > >> that > >> I could try? > >> > >> Thank's in advance, > >> > >> Timo > >> > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > ------------------------------ > > > > Message: 2 > > Date: Wed, 14 Jun 2006 09:08:36 -0500 > > From: "Perry, Margaret" > > Subject: [Histonet] substrates > > To: > > Message-ID: > > > > Content-Type: text/plain; charset=3D"us-ascii" > > > > We are trying to do double staining and are looking for two different > > colors of substrate and we can't use DAB due to melanin. Does anyone > > know of a green stain that is xylene compatible? Vector has Nova Red > > and a blue color but we use hematoxylin as a counterstain. It would > > mean an extra step for us to counterstain in Methyl Green > > > > We use a DAKO autostainer. > > > > > > > > Margaret Perry HT(ASCP) > > > > South Dakota State University > > > > Animal Research and Diagnostic Lab > > > > Brookings SD 57007 > > > > Margaret.Perry@sdstate.edu > > > > > > > > > > > > ------------------------------ > > > > Message: 3 > > Date: Wed, 14 Jun 2006 10:16:10 -0400 > > From: "Conlon, Paula F." > > > > Subject: [Histonet] toe nails > > To: "Histonet \(E-mail\)" > > Message-ID: > > > > Content-Type: text/plain; charset=3D"iso-8859-1" > > > > Hi Histonetters, does anyone have a good procedure for sectioning > nails= > =20 > > and keeping them on the slides during h&e staining and pas > staining?Mos= > t=20 > > of the time our sections wash off. Thanks for your help. > > > > > > See our web page at http://www.lahey.org for a full directory of Lahey=20 > > sites, staff, services and career opportunities. > > > > THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS=20 > > ADDRESSED. IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL > = > AND=20 > > EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If you are not the > intende= > d=20 > > recipient, your use of this message for any purpose is strictly=20 > > prohibited. If you have received this communication in error, please=20 > > delete the message and notify the sender so that we may correct our=20 > > records. > > > > > > ------------------------------ > > > > Message: 4 > > Date: Wed, 14 Jun 2006 07:23:19 -0700 (PDT) > > From: Rene J Buesa > > Subject: Re: [Histonet] substrates > > To: "Perry, Margaret" , > > histonet@lists.utsouthwestern.edu > > Message-ID: <20060614142319.7044.qmail@web61216.mail.yahoo.com> > > Content-Type: text/plain; charset=3Diso-8859-1 > > > > Margaret: > > Confronted with that situation I would eliminate the melanine > better.=20 > > Oxidize it with 0.1% potassium permanganate for 2 hours; wash and > remov= > e=20 > > the oxidized melanine with 0.3% oxalic acid followed by washing. The=20 > > section should be free of melanine pigment/colour afterwards and you > ca= > n=20 > > use DAB (for brown) and DAB + Ni salts for dark blue as double > substrat= > es. > > Hope this will help you! > > Ren=E9 J. > > > > "Perry, Margaret" wrote: > > We are trying to do double staining and are looking for two different > > colors of substrate and we can't use DAB due to melanin. Does anyone > > know of a green stain that is xylene compatible? Vector has Nova Red > > and a blue color but we use hematoxylin as a counterstain. It would > > mean an extra step for us to counterstain in Methyl Green > > > > We use a DAKO autostainer. > > > > > > > > Margaret Perry HT(ASCP) > > > > South Dakota State University > > > > Animal Research and Diagnostic Lab > > > > Brookings SD 57007 > > > > Margaret.Perry@sdstate.edu > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > __________________________________________________ > > Do You Yahoo!? > > Tired of spam? Yahoo! Mail has the best spam protection around > > http://mail.yahoo.com > > > > ------------------------------ > > > > Message: 5 > > Date: Wed, 14 Jun 2006 07:25:13 -0700 (PDT) > > From: Rene J Buesa > > Subject: Re: [Histonet] toe nails > > To: "Conlon, Paula F." > > , "Histonet > > \(E-mail\)" > > Message-ID: <20060614142513.15660.qmail@web61219.mail.yahoo.com> > > Content-Type: text/plain; charset=3Diso-8859-1 > > > > Paula: > > I am forwarding privately a summary on Toenails I prepared recently > tha= > t I=20 > > hope will answer your questions. > > Ren=E9 J. > > > > "Conlon, Paula F." > > wrote: > > Hi Histonetters, does anyone have a good procedure for sectioning > nails= > =20 > > and keeping them on the slides during h&e staining and pas > staining?Mos= > t=20 > > of the time our sections wash off. Thanks for your help. > > > > > > See our web page at http://www.lahey.org for a full directory of Lahey=20 > > sites, staff, services and career opportunities. > > > > THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS=20 > > ADDRESSED. IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL > = > AND=20 > > EXEMPT FROM DISCLOSURE UNDER APPLICABLE LAW. If you are not the > intende= > d=20 > > recipient, your use of this message for any purpose is strictly=20 > > prohibited. If you have received this communication in error, please=20 > > delete the message and notify the sender so that we may correct our=20 > > records. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > __________________________________________________ > > Do You Yahoo!? > > Tired of spam? Yahoo! Mail has the best spam protection around > > http://mail.yahoo.com > > > > ------------------------------ > > > > Message: 6 > > Date: Wed, 14 Jun 2006 09:41:48 -0500 > > From: "Chris Pomajzl" > > Subject: Re: [Histonet] toe nails > > To: "HISTONET" > > Message-ID: <002e01c68fc0$b2fb6330$26fca8c0@CSP> > > Content-Type: text/plain; charset=3D"iso-8859-1" > > > > Good question. We have dealt with this issue for many years, and we > hav= > e > > come up with a solution that has worked for some time. > > > > First of all, sections are picked up on "+" coated slides that > have bee= > n > > smeared with egg albumin. We separate eggs from the grocery store and > k= > eep=20 > > a > > stock of the egg white albumin in a bottle. Maybe 100ml at a time, > keep= > it > > refrigerated. When cutting nails, put 2-3 drops of albumin on the > slide= > =20 > > and > > smear to cover all of the glass. > > > > We cut 3 sections of the nail specimen. 1 for H&E, 1 for PAS, and 1 > ext= > ra > > for back-up. We bake the slides at 55'C overnight, and then at 70'C > for= > 20 > > minutes prior to staining the following day. > > > > This seems to work very well for us. > > > > ----- Original Message -----=20 > > From: "Conlon, Paula F." > > > > To: "Histonet (E-mail)" > > Sent: Wednesday, June 14, 2006 9:16 AM > > Subject: [Histonet] toe nails > > > > > > Hi Histonetters, does anyone have a good procedure for sectioning > nails= > =20 > > and > > keeping them on the slides during h&e staining and pas staining?Most > of= > =20 > > the > > time our sections wash off. Thanks for your help. > > > > > > See our web page at http://www.lahey.org for a full directory of Lahey > > sites, staff, services and career opportunities. > > > > THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS=20 > > ADDRESSED. > > IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND > EXEMPT=20 > > FROM > > DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended > recipient,= > =20 > > your > > use of this message for any purpose is strictly prohibited. If you have > > received this communication in error, please delete the message and > not= > ify > > the sender so that we may correct our records. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > ------------------------------ > > > > Message: 7 > > Date: Wed, 14 Jun 2006 08:11:41 -0700 > > From: Maria Mejia > > Subject: [Histonet] ScyTek's serum free pro-block protein > > To: histonet@lists.utsouthwestern.edu > > Message-ID: > > Content-Type: text/plain; charset=3DUS-ASCII; delsp=3Dyes; > format=3Dflo= > wed > > > > To anyone who using ScyTek's serum free pro-block protein, please let > > me know > > what you think of it. In IHC, do you (only) use it as a diluent for > > the antibodies or also > > use it in the washes? Any information you can provide will be greatly > > appreciated. > > > > Yours > > > > Maria Bartola Mejia > > UCSF > > Depart. of Neurosurgery > > San Francisco, CA 94103 > > > > > > > > ------------------------------ > > > > Message: 8 > > Date: Wed, 14 Jun 2006 11:51:21 -0400 > > From: "Anthony F. Boris" > > Subject: RE: [Histonet] toe nails > > To: "HISTONET" > > Message-ID: > > Content-Type: text/plain; charset=3D"utf-8" > > > > We use charged slides from Premiere. keep in 65 degree oven for 30 > minu= > tes=20 > > and stain. We have not had any fall off since we switched to these > slid= > es.=20 > > When cutting you can use a 5% solution of KOh for a "surface > decal". Th= > is=20 > > breaks down the keratin a little bit (same stuff as in NAIR). Helps to > = > get=20 > > decent sectioning. > > > > Tony > > > > -----Original Message-----=20 > > From: Chris Pomajzl [mailto:cpomajzl@cpllabs.com] > > Sent: Wed 6/14/2006 10:41 AM > > To: HISTONET > > Cc: > > Subject: Re: [Histonet] toe nails > > > > > > > > Good question. We have dealt with this issue for many years, and we > hav= > e > > come up with a solution that has worked for some time. > > > > First of all, sections are picked up on "+" coated slides that > have bee= > n > > smeared with egg albumin. We separate eggs from the grocery store and > k= > eep=20 > > a > > stock of the egg white albumin in a bottle. Maybe 100ml at a time, > keep= > it > > refrigerated. When cutting nails, put 2-3 drops of albumin on the > slide= > =20 > > and > > smear to cover all of the glass. > > > > We cut 3 sections of the nail specimen. 1 for H&E, 1 for PAS, and 1 > ext= > ra > > for back-up. We bake the slides at 55'C overnight, and then at 70'C > for= > 20 > > minutes prior to staining the following day. > > > > This seems to work very well for us. > > > > ----- Original Message ----- > > From: "Conlon, Paula F." > > > > To: "Histonet (E-mail)" > > Sent: Wednesday, June 14, 2006 9:16 AM > > Subject: [Histonet] toe nails > > > > > > Hi Histonetters, does anyone have a good procedure for sectioning > nails= > =20 > > and > > keeping them on the slides during h&e staining and pas staining?Most > of= > =20 > > the > > time our sections wash off. Thanks for your help. > > > > > > See our web page at http://www.lahey.org for a full directory of Lahey > > sites, staff, services and career opportunities. > > > > THIS MESSAGE IS INTENDED FOR THE USE OF THE PERSON TO WHOM IT IS=20 > > ADDRESSED. > > IT MAY CONTAIN INFORMATION THAT IS PRIVILEGED, CONFIDENTIAL AND > EXEMPT=20 > > FROM > > DISCLOSURE UNDER APPLICABLE LAW. If you are not the intended > recipient,= > =20 > > your > > use of this message for any purpose is strictly prohibited. If you have > > received this communication in error, please delete the message and > not= > ify > > the sender so that we may correct our records. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > > ------------------------------ > > > > Message: 9 > > Date: Wed, 14 Jun 2006 08:52:10 -0700 (PDT) > > From: Geoffrey White > > Subject: [Histonet] Manual cover slip method - DiscoverSlip? > > To: histonet@lists.utsouthwestern.edu > > Message-ID: <20060614155210.68428.qmail@web36513.mail.mud.yahoo.com> > > Content-Type: text/plain; charset=3Diso-8859-1 > > > > > > Dear all, > > > > > > > > at the time our research group is looking for new manual method for > cov= > er=20 > > our slides during the hybridization. In the past we used standard > glass= > =20 > > cover slips but we are not really satisfied with this method. > > > > Just I saw at Biocompare a new method with the name DiscoverSlip. > This=20 > > technology looks very interesting. > > > > Have any work with DiscoverSlip or saw this technology working? > > > > > > > > Thanks > > > > Geoffrey > > > > __________________________________________________ > > Do You Yahoo!? > > Tired of spam? Yahoo! Mail has the best spam protection around > > http://mail.yahoo.com > > > > ------------------------------ > > > > Message: 10 > > Date: Wed, 14 Jun 2006 12:01:42 -0400 > > From: Susan Q Wells > > Subject: Re: [Histonet] substrates > > To: Rene J Buesa > > Cc: histonet@lists.utsouthwestern.edu, > "Perry, Margaret" > > > > Message-ID: <449032E6.8030300@bms.com> > > Content-Type: text/plain; format=3Dflowed; charset=3DISO-8859-1 > > > > You may lose your epitopes with this procedure, if so 10% H202 in PBS > > for 30 minutes may lighten the melanin pigment enough > > to view your chromagen. I recently used Vulcan Fast Red and Bajoran > > Purple from Biocare Medical with great success where melanin pigment > wa= > s > > present. > > > > Sue Wells HT(ASCP), QIHC > > > > Rene J Buesa wrote: > > > >>Margaret: > >> Confronted with that situation I would eliminate the melanine > better.=20 > >> Oxidize it with 0.1% potassium permanganate for 2 hours; wash and > remo= > ve=20 > >> the oxidized melanine with 0.3% oxalic acid followed by washing. > The=20 > >> section should be free of melanine pigment/colour afterwards and you > c= > an=20 > >> use DAB (for brown) and DAB + Ni salts for dark blue as double=20 > >> substrates. > > > > =3D=3D=3D message truncated =3D=3D=3D > > > > > > --------------------------------- > > Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and > 30+=20 > > countries) for 2=A2/min or less. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet=20 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo.rogerson <@t> waht.swest.nhs.uk Mon Jun 19 02:13:19 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Jun 19 02:12:54 2006 Subject: [Histonet] Who is this MORON? Message-ID: Male, without doubt, American most likely; note the dubious grammar. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From vanann702 <@t> skmc.gov.ae Mon Jun 19 02:39:56 2006 From: vanann702 <@t> skmc.gov.ae (Anne Van Binsbergen) Date: Mon Jun 19 02:37:18 2006 Subject: [Histonet] Who is this MORON? Message-ID: Some idiot who has no clue what this list-server is all about and bit of a little more than he could chew by subscribing - ended up with an overfull inbox - and emails being rejected!! If you can't stand the heat get out of the kitchen. Annieinarabia -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: Monday, June 19, 2006 11:13 AM To: 'Joe Nocito'; Hofecker, Jennifer L; histonet Subject: RE: [Histonet] Who is this MORON? Male, without doubt, American most likely; note the dubious grammar. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b003046 <@t> nf.au.dk Mon Jun 19 05:06:37 2006 From: b003046 <@t> nf.au.dk (b003046@nf.au.dk) Date: Mon Jun 19 05:06:45 2006 Subject: [Histonet] Lineage negative markers Message-ID: <1150711597.4496772d361a9@webmail.nf.au.dk> Hi, I would like to exclude lineage positive cells using flow cytometry. Does anybody know a mixture of lineage markers conjugated with FITC that react with the rat? Any help will be appreciated:) Cheers Mette K. Hagensen B-research, Skejby Hospital Aarhus University Hospital Denmark From juan.gutierrez <@t> christushealth.org Mon Jun 19 07:21:02 2006 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Mon Jun 19 07:21:13 2006 Subject: [Histonet] Myo D1 Message-ID: Yes, we switched to myogenin. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Tracey.Lenek@CLS.ab.ca Sent: Friday, June 16, 2006 4:08 PM To: histonet@lists.utsouthwestern.edu Cc: bartczak-mckay.joanna@cls.ab.ca Subject: [Histonet] Myo D1 Hi, We are trying to develop Dako's Myo D1 on the Ventana Benchmark. We are finding alot of cross reactivity with macrophages, endothelial cells, etc. Has anyone had the same experience with this antibody or better yet developed a consistent protocol? Thanks in advance. Tracey CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From froyer <@t> bitstream.net Mon Jun 19 10:01:36 2006 From: froyer <@t> bitstream.net (Ford Royer) Date: Mon Jun 19 10:01:50 2006 Subject: [Histonet] Inflammatory Breast Cancer (IBC) In-Reply-To: Message-ID: <005a01c693b1$45116460$7701a80a@Ford> I just received an email from a subscriber to this list with a video attachment of a local newscast regarding Inflammatory Breast Cancer (IBC). I had never heard of this condition and I was wondering (1) if it has been around for some time and (2) if there is/are special diagnostic procedures, other than mammogram (i.e. IHC) for it. Any history on this disease would be appreciated. I have not heard of it before now. Link to newscast: ~ Ford Ford M. Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL: 612-839-1046 Phone: 763-542-8725 Fax: 763-546-4830 eMail: froyer@bitstream.net From victor <@t> pathology.washington.edu Mon Jun 19 10:09:02 2006 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Mon Jun 19 10:09:09 2006 Subject: [Histonet] Inflammatory Breast Cancer (IBC) In-Reply-To: <005a01c693b1$45116460$7701a80a@Ford> References: <005a01c693b1$45116460$7701a80a@Ford> Message-ID: <4496BE0E.6050500@pathology.washington.edu> Here is the link to the news story. I couldn't get the video to work. http://www.komotv.com/news/story.asp?ID=43313 Victor Ford Royer wrote: >I just received an email from a subscriber to this list with a video >attachment of a local newscast regarding Inflammatory Breast Cancer (IBC). >I had never heard of this condition and I was wondering (1) if it has been >around for some time and (2) if there is/are special diagnostic procedures, >other than mammogram (i.e. IHC) for it. > >Any history on this disease would be appreciated. I have not heard of it >before now. > >Link to newscast: > > >~ Ford > >Ford M. Royer, MT(ASCP) >Histology Product Manager >Minnesota Medical, Inc. >7177 Madison Ave. W. >Golden Valley, MN 55427-3601 >CELL: 612-839-1046 >Phone: 763-542-8725 >Fax: 763-546-4830 >eMail: froyer@bitstream.net > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From HornHV <@t> archildrens.org Mon Jun 19 10:34:41 2006 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon Jun 19 10:35:31 2006 Subject: [Histonet] Inflammatory Breast Cancer (IBC) In-Reply-To: <005a01c693b1$45116460$7701a80a@Ford> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6C1B@EMAIL.archildrens.org> Inflammatory breast cancer is not new. It is a serious high-risk cancer. ------------------------------------------------------------------------ -------- From the IBC website: There is more than one kind of breast cancer. We have been taught and are reminded frequently by public service announcements and by the medical community that when a woman discovers a lump on her breast she should go to the doctor immediately. Inflammatory breast cancer usually grows in nests or sheets, rather than as a confined, solid tumor and therefore can be diffuse throughout the breast with no palpable mass. The cancer cells clog the lymphatic system just below the skin. Lymph node involvement is assumed. Increased breast density compared to prior mammograms should be considered suspicious. You Don't Have to Have a Lump to Have Breast Cancer. Some women who have inflammatory breast cancer may remain undiagnosed for long periods, even while seeing their doctor to learn the cause of her symptoms. The symptoms are similar to mastitis, a breast infection and some doctors, not recognizing IBC, will prescribe antibiotics. If a response to antibiotics is not apparent after a week, a biopsy should be performed or a referral to a breast specialist is warranted. Age 52: Median age at time of diagnosis of IBC ... versus, Age 62: Median age at time of diagnosis of Breast Cancer. A surprising portion of young women with IBC had their first symptoms during pregnancy or lactation. The misconception that these young women are at lower risk for breast cancer and the fact that IBC is the most aggressive form of breast cancer may result in metastases when the diagnosis is made. One or more of the following are Typical Symptoms of IBC: Swelling, usually sudden, sometimes a cup size in a few days Itching Pink, red, or dark colored area (called erythema) sometimes with texture similar to the skin of an orange (called peau d'orange) Ridges and thickened areas of the skin What appears to be a bruise that does not go away Nipple retraction Nipple discharge, may or may not be bloody Breast is warm to the touch Breast pain (from a constant ache to stabbing pains) Change in color and texture of the aureole View pictures showing common presentation of some of these symptoms. Read what patients write about their own symptoms prior to diagnosis. View a 4:23 minute video about IBC shown on NBC5 in Chicago. These Symptoms May Be Present in Benign Breast Disorders. See your doctor if you have any of these symptoms. Inflammatory Breast Cancer is typically abbreviated as IBC. Non-inflammatory breast cancer may include in its diagnosis the terms "in situ breast cancer," "infiltrating breast cancer," or "invasive breast cancer" all of which may be abbreviated with "ibc," but those terms alone do not specify inflammatory breast cancer. To add to the possible confusion, the diagnosis may include more that one kind of breast cancer; for example "inflammatory breast cancer, invasive ductal carcinoma, and mucinous carcinoma" all in the same breast. So if a person you know has been described as having IBC or ibc, it may be well to ask what that is abbreviating, since it may not be "inflammatory breast cancer" and therefore the symptoms and other information presented here may not apply. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ford Royer Sent: Monday, June 19, 2006 10:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Inflammatory Breast Cancer (IBC) I just received an email from a subscriber to this list with a video attachment of a local newscast regarding Inflammatory Breast Cancer (IBC). I had never heard of this condition and I was wondering (1) if it has been around for some time and (2) if there is/are special diagnostic procedures, other than mammogram (i.e. IHC) for it. Any history on this disease would be appreciated. I have not heard of it before now. Link to newscast: ~ Ford Ford M. Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL: 612-839-1046 Phone: 763-542-8725 Fax: 763-546-4830 eMail: froyer@bitstream.net _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From n.cragg <@t> epistem.co.uk Mon Jun 19 10:36:39 2006 From: n.cragg <@t> epistem.co.uk (Nicola Cragg) Date: Mon Jun 19 10:36:46 2006 Subject: [Histonet] Leucocyte Staining - is Giemsa enough? Message-ID: Hi All, Is there a histological stain which stains for all leucocytes and can this stain be used as a counterstain after DAB as part of an IHC stain? I have a working protocol for May-Grunwald's-Giemsa which I have used successfully on frozen sections. However, I'm not sure whether a) this can be used as a counterstain on FFPE after DAB and b) whether it stains enough types of leucocytes to describe it as a "leucocyte counterstain". I have read with interest the post's on Giemsa & Wright's stain but I'm still unsure as to whether I'm on the right lines. I've also been reading up on leucocytes to try & figure this out, which gets quite confusing in itself. Do I really need to be doing a double IHC with a leucocytes marker, e.g. CD45/ Leucocyte Common Antigen? I would really appreciate any help with this. Thank you in advance, Nicola Cragg Manchester, UK This message has been scanned for viruses by BlackSpider MailControl - www.blackspider.com From LewisS <@t> pediatrics.ohio-state.edu Thu Jun 15 12:48:22 2006 From: LewisS <@t> pediatrics.ohio-state.edu (Lewis, Sarah) Date: Mon Jun 19 13:15:49 2006 Subject: [Histonet] Freezing small pieces of skeletal muscle tissue Message-ID: <714B9F12B4E18C4C843B66E8E190F2AD0134087D@res2k3ms01.CRII.ORG> Jen, This is the protocol we send to outside institutions. I hope it will help. You mentioned you wait until the 2-methylbutane/isopentane gets slushy... This we do not do. I listen for the "boiling" sound to stop(so to speak). Your solution should be clear.. Not cloudy. You will start to see little white crystals form... This is when you put your tissue in the 2-methylbutane suspended in liquid nitrogen. A 0.5x0.5x1.0 pc of muscle should be in for 30sec. Tissue any smaller, cut your time down by 5 sec according to size. Hold tissue in cryostat or -20/-40 until tissue is dry. Wrap with foil and store in -80. If you have any other questions just give me a call. I hope this helps with your problem. Take Care, Sarah Sarah E. Lewis Histotechnician Mendell Neuromuscular Lab Childrens Research Center for Gene Therapy 700 Childrens Dr Rm WA3112 Columbus OH 43205 (614)-722-2204 LewisS@ccri.net -----Original Message----- From: Katri Tuomala [mailto:katri@cogeco.ca] Sent: Sunday, June 11, 2006 7:29 PM To: Dearolf, Jennifer; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Freezing small pieces of skeletal muscle tissue Jenn, I'm sure you'll get some good advice after the weekend from people who do this all the time. My initial thought is, that your specimen should go from isopentane directly to -70C, not -20C, unless you cut it then. When you transfer the tissue to -70C, ice ctystal artifact will form, when tissue freezes slowly from -20 to -70. Just a thought... ----- Original Message ----- From: "Dearolf, Jennifer" To: Sent: Friday, June 09, 2006 3:20 PM Subject: [Histonet] Freezing small pieces of skeletal muscle tissue Greetings, Histonetters, I realize that freezing skeletal muscle tissue has been a topic many times before, but in going through the archives, I could not find anyone that was using the method that we are (and maybe that's the problem, since we are having issues with freezing artifact). Thus, I am writing the list to see if anyone has any suggestions for how we might modify our methods to have better results. We collect our tissue fresh and mount it in 5% gum tragacanth on cork blocks (6mm thick). To freeze the tissue, we put some isopentane in a frozen juice can (with the juice removed, of course!) and place the juice can in liquid nitrogen. The liquid nitrogen is contained in a small, styrofoam cooler. When the isopentane gets slushy, we freeze our samples for 60 seconds. We then toss the frozen samples into a cryostat set at -21C. Once the samples have warmed up and any liquid isopentane has evaporated, we wrap our samples in parafilm and store in cardboard boxes in a -70C freezer. To cut, we move the boxes back into the cryostat and allow to wam up to -21C for approximately an hour. Then, we cut. I used this method successfully with larger pieces of muscle, but we are now attempting to freeze some mouse and guinea pig muscles (diaphragm, scalenus, rectus thoracis, and rectus abdominus), and we are getting a ton of freezing artifact. To try to prevent the artifact, we reduced the freezing time to 20 seconds. We also tried wrapping our samples in small pieces of liver. Neither method seems to work consistently. I am at my wits end. Everytime I think we have the freezing artifact beaten, it comes back with a vengence. I would appreciate any advice. I will keep track of the temperature of the isopentane, since this seems to be an important variable to control (according to the discussions in the archives). I would like to ask, however, should the temperature be -150 or -160C when you freeze the muscle? Thanks for your help! Sincerely, Jenn Jennifer Dearolf, Ph.D. Assistant Professor Biology Department Hendrix College 1600 Washington Avenue Conway, AR 72032 (501) 450-4530 (office) (501) 450-4547 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ccrowder <@t> vetmed.lsu.edu Fri Jun 16 16:46:15 2006 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Mon Jun 19 13:15:51 2006 Subject: [Histonet] NSH Convention Poster Abstracts Message-ID: The NSH is still accepting poster abstracts for the 2006 Convention in Phoenix this Sept. The abstract deadline as been extended to June 30 to allow members more time to submit abstracts. The poster abstract form, directions for making a poster and the criteria for judging the posters are available on line at www.NSH.org. Click on convention, then click on poster for abstract form. Poster content can come from any area - new techniques, review of old techniques, techniques used for different purposes, history in histotechnology, anything you do as a tech can be shown on a poster. If you have any questions concerning the posters, contact me or the NSH office. We really want you to submit - and contrary to what your nerves are saying, it is fun and really serves a good educational purpose. Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 From RSRICHMOND <@t> aol.com Mon Jun 19 14:25:16 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Mon Jun 19 14:25:26 2006 Subject: [Histonet] Inflammatory Breast Cancer (IBC) Message-ID: <509.7907a7.31c8541c@aol.com> Inflammatory carcinoma is a clinical diagnosis: The patient presents with what looks like cellulitis of the skin of the breast - the area that looks inflammatory is deep red and very well demarcated, just like bacterial cellulitis. The redness is the result of invasion of lymphatics in the dermis by an underlying breast cancer. Just seeing dermal lymphatic invasion microscopically isn't enough - the clinical presentation is required to make the diagnosis. Inflammatory carcinoma has an extremely bad prognosis. Until just a few years ago, all patients presenting with inflammatory carcinoma died of cancer very rapidly. I think that now a few patients survive it, but I don't have any numbers. Bob Richmond Knoxville TN and Gastonia NC From AFeatherstone <@t> KaleidaHealth.Org Mon Jun 19 15:24:15 2006 From: AFeatherstone <@t> KaleidaHealth.Org (Featherstone, Annette) Date: Mon Jun 19 15:24:21 2006 Subject: [Histonet] Ventana Bar Codes and Dispenser Lables Message-ID: <9B4A77DF11463E4FB723D484214AE9BCA552E6@KALEXMB02.KaleidaHealth.org> Is anyone interested in purchasing Ventana ES Barcode labels and Dipenser Bar Codes? Approx 60 Dispenser Labels and many slide barcodes. Annette Featherstone HT/MLT ASCP, MT (HEW) Supervisor Anatomic Pathology Kaleida Health, Buffalo General Hospital 100 High Street Buffalo NY 14203 716-859-2625 FAX: 716-859-1853 CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From anh2006 <@t> med.cornell.edu Mon Jun 19 16:20:25 2006 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Mon Jun 19 16:19:21 2006 Subject: [Histonet] Far red fluorophore counterstain Message-ID: What is everyone's favorite Far Red fluorophore counterstain? Some folks in my lab are having some issues with TOPRO-3 these days and are getting frustrated so I am looking for something more user friendly. Any suggestions? -- From Jacqueline.Malam <@t> rli.mbht.nhs.uk Tue Jun 20 03:02:15 2006 From: Jacqueline.Malam <@t> rli.mbht.nhs.uk (Malam Jacqueline) Date: Tue Jun 20 03:04:12 2006 Subject: [Histonet] HMLH1 ON PARAFFIN SECTIONS Message-ID: Has anyone had any experience of using this antibody on paraffin sections? I have located a supplier (Serotec) - are there any others out there? Jacqui Malam Lancaster UK DISCLAIMER: This e-mail is confidential and privileged. If you are not the intended recipient please accept our apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform postmaster@rli.mbht.nhs.uk that this message has gone astray before deleting it. Comments or opinions expressed in this email are those of their respective contributors only. The views expressed do not represent the views of the Trust, its management or employees. University Hospitals of Morecambe Bay NHS Trust is not responsible and disclaims any and all liability for the content of comments written within.Thank you for your co-operation. From kappeler <@t> patho.unibe.ch Tue Jun 20 03:52:56 2006 From: kappeler <@t> patho.unibe.ch (Andi Kappeler) Date: Tue Jun 20 03:53:35 2006 Subject: [Histonet] HMLH1 ON PARAFFIN SECTIONS References: Message-ID: <008b01c69446$fea2edf0$27955c82@patho.unibe.ch> Hi Jacqui we use mo-a-hMLH1, clone G168-15, B&D Biosciences Cat.Nr. 554072. Working conc. is 2.5 ug Ig/ml after HIER in pressure cooker / citrate. Hope this helps. Andi Kappeler Institute of Pathology, University of Bern, Switzerland ----- Original Message ----- From: "Malam Jacqueline" To: Sent: Tuesday, June 20, 2006 10:02 AM Subject: [Histonet] HMLH1 ON PARAFFIN SECTIONS > Has anyone had any experience of using this antibody on paraffin sections? > I > have located a supplier (Serotec) - are there any others out there? > > Jacqui Malam > Lancaster > UK > > > > DISCLAIMER: This e-mail is confidential and privileged. If you are not the > intended recipient please accept our apologies; please do not disclose, > copy > or distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. Please > inform postmaster@rli.mbht.nhs.uk that this message has gone astray before > deleting it. Comments or opinions expressed in this email are those of > their respective contributors only. The views expressed do not represent > the > views of the Trust, its management or employees. University Hospitals of > Morecambe Bay NHS Trust is not responsible and disclaims any and all > liability for the content of comments written within.Thank you for your > co-operation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JGREWE <@t> OhioHealth.com Tue Jun 20 04:09:07 2006 From: JGREWE <@t> OhioHealth.com (JGREWE@OhioHealth.com) Date: Tue Jun 20 04:09:23 2006 Subject: [Histonet] Janci Wellborn - Milestone MW processors In-Reply-To: Message-ID: We demo'd the smaller RHS-1 with routine surgical and breast tissue. The pathologists liked the results enough to encourage the purchase of three Pathos units. They were installed last week and we will start to use them for our biopsy runs this week. You can contact me directly with future questions if you would like. jgrewe@OhioHealth.com From kappeler <@t> patho.unibe.ch Tue Jun 20 05:49:18 2006 From: kappeler <@t> patho.unibe.ch (Andi Kappeler) Date: Tue Jun 20 05:49:57 2006 Subject: [Histonet] HMLH1 ON PARAFFIN SECTIONS References: Message-ID: <00bb01c69457$4033f3d0$27955c82@patho.unibe.ch> Seems like they have changed the Cat.Nr. from 554072 to 551091 (clone G168-15, however at lower concentration than before). You should find the Data Sheet under http://www.bdbiosciences.com/external_files/pm/doc/tds/cell_bio/live/web_enabled/1327GN_551091.pdf Clone G168-15 works significantly better than clone G168-728 (we had a lot of trouble with 728) and is no longer recommended for IHC by BD Pharmingen. Good luck Andi ----- Original Message ----- From: "Malam Jacqueline" To: "'Andi Kappeler'" Sent: Tuesday, June 20, 2006 11:46 AM Subject: RE: [Histonet] HMLH1 ON PARAFFIN SECTIONS > Many thanks - tried to find it on B&D but was redirected to Clontech who > didn't have the one you mentioned. I have since found 3 clones at > Invitrogen > and one at BD Pharmingen, clone G168-728, cat no 554073 - could this be > the > same one you mentioned - it's very close. We use the Ventana Benchmark XT > so > plenty of choice when optimising. > Cheers > Jacqui > > -----Original Message----- > From: Andi Kappeler [mailto:kappeler@patho.unibe.ch] > Sent: 20 June 2006 09:53 > To: Malam Jacqueline; Histonet > Subject: Re: [Histonet] HMLH1 ON PARAFFIN SECTIONS > > Hi Jacqui > > we use mo-a-hMLH1, clone G168-15, B&D Biosciences Cat.Nr. 554072. Working > conc. is 2.5 ug Ig/ml after HIER in pressure cooker / citrate. Hope this > helps. > > Andi Kappeler > Institute of Pathology, University of Bern, Switzerland > > ----- Original Message ----- > From: "Malam Jacqueline" > To: > Sent: Tuesday, June 20, 2006 10:02 AM > Subject: [Histonet] HMLH1 ON PARAFFIN SECTIONS > > >> Has anyone had any experience of using this antibody on paraffin >> sections? > >> I >> have located a supplier (Serotec) - are there any others out there? >> >> Jacqui Malam >> Lancaster >> UK >> >> >> >> DISCLAIMER: This e-mail is confidential and privileged. If you are not >> the >> intended recipient please accept our apologies; please do not disclose, >> copy >> or distribute information in this e-mail or take any action in reliance >> on >> its contents: to do so is strictly prohibited and may be unlawful. Please >> inform postmaster@rli.mbht.nhs.uk that this message has gone astray >> before >> deleting it. Comments or opinions expressed in this email are those of >> their respective contributors only. The views expressed do not represent >> the >> views of the Trust, its management or employees. University Hospitals of >> Morecambe Bay NHS Trust is not responsible and disclaims any and all >> liability for the content of comments written within.Thank you for your >> co-operation. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > DISCLAIMER: This e-mail is confidential and privileged. If you are not the > intended recipient please accept our apologies; please do not disclose, > copy > or distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. Please > inform postmaster@rli.mbht.nhs.uk that this message has gone astray before > deleting it. Comments or opinions expressed in this email are those of > their respective contributors only. The views expressed do not represent > the > views of the Trust, its management or employees. University Hospitals of > Morecambe Bay NHS Trust is not responsible and disclaims any and all > liability for the content of comments written within.Thank you for your > co-operation. > > From louise.renton <@t> gmail.com Tue Jun 20 05:56:46 2006 From: louise.renton <@t> gmail.com (louise renton) Date: Tue Jun 20 05:56:51 2006 Subject: [Histonet] coverslip staining jars Message-ID: Hello all, A colleague is loooking for those elusive items known as "Columbia jars" or coverslip staining jars (they look like miniature glass coplin jars)? Lipshaw (now thermos/electro/shandon) used to have them, but I don't see it in the catalogue. thanks -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From jqb7 <@t> cdc.gov Tue Jun 20 06:03:01 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/NCID/VR)) Date: Tue Jun 20 06:03:10 2006 Subject: [Histonet] coverslip staining jars Message-ID: Fisher sells them. They list them as coverslip coplin jars. Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: Tuesday, June 20, 2006 6:57 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] coverslip staining jars Hello all, A colleague is loooking for those elusive items known as "Columbia jars" or coverslip staining jars (they look like miniature glass coplin jars)? Lipshaw (now thermos/electro/shandon) used to have them, but I don't see it in the catalogue. thanks -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rfisher <@t> gbmc.org Tue Jun 20 07:15:02 2006 From: rfisher <@t> gbmc.org (RENEE FISHER) Date: Tue Jun 20 07:15:27 2006 Subject: [Histonet] Hello all, our lab is thinking about getting the microwave processor for bx's and reprocessing tis Message-ID: <4497AE86020000CA00002372@mail.gbmc.org> Hello all, our lab is thinking about getting the microwave processor for bx's and reprocessing tissue. I wanted to get some feed back from those of you who have the MW processor, please feel free to e-mail me your thoughts, also we reprocess a great number of blocks ( fatty breast that has been pushed through for the 24hr turn around) wanted some thoughts on running tissue back to formalin sometimes when we do this the tissue is hard and brittle. Thanks, Renee From Jackie.O'Connor <@t> abbott.com Tue Jun 20 07:24:31 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Tue Jun 20 07:24:58 2006 Subject: [Histonet] Off topic question about Detroit In-Reply-To: <4497AE86020000CA00002372@mail.gbmc.org> Message-ID: Can anyone suggest a hotel near the Detroit zoo for a couple of newbies to the Detroit area? A couple of my adult kids are planning a trip around some business near the zoo - they plan to jump over to Toronto to do some shopping. They have no idea where to stay - or where to stay away from. Any suggestions? Jackie From jqb7 <@t> cdc.gov Tue Jun 20 07:22:23 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/NCID/VR)) Date: Tue Jun 20 07:25:37 2006 Subject: [Histonet] Hello all, our lab is thinking about getting the microwaveprocessor for bx's and reprocessing tis Message-ID: Which MW processor are you looking at? Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of RENEE FISHER Sent: Tuesday, June 20, 2006 8:15 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Hello all,our lab is thinking about getting the microwaveprocessor for bx's and reprocessing tis Hello all, our lab is thinking about getting the microwave processor for bx's and reprocessing tissue. I wanted to get some feed back from those of you who have the MW processor, please feel free to e-mail me your thoughts, also we reprocess a great number of blocks ( fatty breast that has been pushed through for the 24hr turn around) wanted some thoughts on running tissue back to formalin sometimes when we do this the tissue is hard and brittle. Thanks, Renee _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Tue Jun 20 08:19:13 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Jun 20 08:20:10 2006 Subject: [Histonet] Collagen - Type I vs. III Message-ID: <4497BD9102000077000008D7@hcnwgwds01.hh.chs> We just received a request from a surgeon to differentiate type I from type III collagen in a bladder biopsy. Can this be done? If so, how and what are the tissue requirements? Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From eca9 <@t> georgetown.edu Tue Jun 20 08:31:36 2006 From: eca9 <@t> georgetown.edu (Eva Andersson) Date: Tue Jun 20 08:31:51 2006 Subject: [Histonet] IHC with p27? Message-ID: <4497F8B8.9050000@georgetown.edu> Good morning, I want to do some IHC with a Kip1/p27 antibody from BD Transduction laboratories (610241) but I can't find any references to a working concentration. Has anybody used this antibody for IHC? What conditions did you use? Thanks for all your help, Eva Andersson Research Assistant Georgetown University From ja.mitchell <@t> hosp.wisc.edu Tue Jun 20 08:49:25 2006 From: ja.mitchell <@t> hosp.wisc.edu (Mitchell Jean A.) Date: Tue Jun 20 08:49:41 2006 Subject: [Histonet] coverslip staining jars Message-ID: <583D3E9A1E843445BD54E461D1A2F6F3192C1C59@UWHIS-XCHNG2.uwhis.hosp.wisc.edu> Louise: Electron Microscopy Sciences (EMS) and RALamb also carry Columbia Jars for 22 X 22 coverglass staining. Jean Mitchell University of Wisconsin Hospital and Clinics Neuromuscular Laboratory Madison, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of louise renton Sent: Tuesday, June 20, 2006 5:57 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] coverslip staining jars Hello all, A colleague is loooking for those elusive items known as "Columbia jars" or coverslip staining jars (they look like miniature glass coplin jars)? Lipshaw (now thermos/electro/shandon) used to have them, but I don't see it in the catalogue. thanks -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Ebreisch <@t> chsd.org Tue Jun 20 08:50:56 2006 From: Ebreisch <@t> chsd.org (Breisch, Eric) Date: Tue Jun 20 08:51:20 2006 Subject: [Histonet] Chlamydia detection from paraffin embedded tissue Message-ID: <00D7298B3825D511BF540008C75F8A3C0CAD830D@excluster.chsd.org> Does anyone know of a histology laboratory which has testing (immunoperoxidase or IFA) available to detect the presence of Chlamydia in paraffin embedded tissue and would you please send me the information? Your assistance is greatly appreciated. Thank you, Eric A. Breisch From jtcrhb <@t> umr.edu Tue Jun 20 09:02:02 2006 From: jtcrhb <@t> umr.edu (Campbell, John Thomas (UMR-Student)) Date: Tue Jun 20 09:02:09 2006 Subject: [Histonet] histo question Message-ID: <8D80EE169A64FA448EBEDC5812BA7F9B1E8D08@UMR-CMAIL1.umr.edu> I am a grad student at the University of Missouri Rolla and I am working on 3D reconstructions of amphibians. We are trying to slice and stain some specimans that we have so we can do the reconstructions. I need to be able to dye the muscle and bone different colors and if possible stain the cartilage too, but this is not neccisary. Does anyone have any suggestions as to what technique I should use? Thanks John From kemlo.rogerson <@t> waht.swest.nhs.uk Tue Jun 20 09:18:14 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Jun 20 09:17:55 2006 Subject: [Histonet] histo question Message-ID: Alizarin Red technique of Jensch and Brent (Stain Technol., 41:179, 1966) will stain bone red but other tissues are transparent. There are a whole raft of similar techniques using Alizarin Red after KOH maceration but I never stained muscle a different colour. I am assuming you don't want staining on slides but on the gross sliced specimen? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Each one sees what he carries in his heart. --Johann Wolfgang von Goethe This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From TillRenee <@t> uams.edu Tue Jun 20 09:58:29 2006 From: TillRenee <@t> uams.edu (Till, Renee) Date: Tue Jun 20 09:59:05 2006 Subject: [Histonet] Tunel and IHC Message-ID: <11F927674DEBDC43B960809A7403C5D2013C66C4@MAILPED.ad.uams.edu> Has anyone tried to co-localize an IHC and Tunel on cells with fluorescence? I saw tons of messages about Tunel, but nothing about this specifically. It seems to me that in some ways cells would present more of a problem. Another tech (I don't do just cells) is trying to do Tunel and Pten fluorescence on the same slide. He has tried doing the Tunel first and then the Pten, and it seemed to work. He is going to try doing it the other way also to compare. You could do multiple slides, but would it be the same as if you were doing tissues that you could use nearly identical sections for each of the stains and the double? What I'm wondering is what are the problems that occur and how does doing fluorescence factor into it. He mentioned not doing the proteinase K step of the Tunel (because it would interfere with the subsequent Pten staining) and still got staining. Is that valid? To just skip a step that you would normally do? He checks the first stain before starting the second, but doesn't take any photos until both are done. Shouldn't you at least take a photo when checking the first stain to see if the second affects it any? Just seems like he has too many variables. Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72002 Office (501)364-2785 Fax (501)364-3161 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From denise.woodward <@t> uconn.edu Tue Jun 20 10:00:02 2006 From: denise.woodward <@t> uconn.edu (Woodward, Denise) Date: Tue Jun 20 10:00:09 2006 Subject: [Histonet] FFPE Brucella control needed Message-ID: Anyone out there that can spare a Formalin-fixed, Paraffin-embedded Tissue control block for Brucella. Marine Mammal tissue is preferred but we'll take whatever we can get! Many Thanks, Denise Long Woodward, MS, HT (ASCP), HTL, QIHC University of Connecticut Dept. of Pathobiology and Veterinary Sciences 61 N. Eagleville Road, Unit 3089 Storrs, CT 06269-3089 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andi Kappeler Sent: Tuesday, June 20, 2006 4:53 AM To: Malam Jacqueline; Histonet Subject: Re: [Histonet] HMLH1 ON PARAFFIN SECTIONS Hi Jacqui we use mo-a-hMLH1, clone G168-15, B&D Biosciences Cat.Nr. 554072. Working conc. is 2.5 ug Ig/ml after HIER in pressure cooker / citrate. Hope this helps. Andi Kappeler Institute of Pathology, University of Bern, Switzerland ----- Original Message ----- From: "Malam Jacqueline" To: Sent: Tuesday, June 20, 2006 10:02 AM Subject: [Histonet] HMLH1 ON PARAFFIN SECTIONS > Has anyone had any experience of using this antibody on paraffin sections? > I > have located a supplier (Serotec) - are there any others out there? > > Jacqui Malam > Lancaster > UK > > > > DISCLAIMER: This e-mail is confidential and privileged. If you are not the > intended recipient please accept our apologies; please do not disclose, > copy > or distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. Please > inform postmaster@rli.mbht.nhs.uk that this message has gone astray before > deleting it. Comments or opinions expressed in this email are those of > their respective contributors only. The views expressed do not represent > the > views of the Trust, its management or employees. University Hospitals of > Morecambe Bay NHS Trust is not responsible and disclaims any and all > liability for the content of comments written within.Thank you for your > co-operation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kappeler <@t> patho.unibe.ch Tue Jun 20 10:05:32 2006 From: kappeler <@t> patho.unibe.ch (Andi Kappeler) Date: Tue Jun 20 10:06:11 2006 Subject: [Histonet] IHC with p27? References: <4497F8B8.9050000@georgetown.edu> Message-ID: <010f01c6947b$0c1db6c0$27955c82@patho.unibe.ch> Hi Eva We used to use mo-a-Kip1/p27, clone 57, at approx. 1 ug Ig/ml after HIER in pressure cooker, citrate. As this was several years ago, we did not test HIER with high pH buffers. Hope this helps. Andi Kappeler Institute of Pathology, University of Bern, Switzerland ----- Original Message ----- From: "Eva Andersson" To: Sent: Tuesday, June 20, 2006 3:31 PM Subject: [Histonet] IHC with p27? > Good morning, > I want to do some IHC with a Kip1/p27 antibody from BD Transduction > laboratories (610241) but I can't find any references to a working > concentration. Has anybody used this antibody for IHC? What conditions did > you use? > Thanks for all your help, > Eva Andersson > Research Assistant Georgetown University > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From tp2 <@t> medicine.wisc.edu Tue Jun 20 10:23:30 2006 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Tue Jun 20 10:24:07 2006 Subject: [Histonet] Tunel and IHC Message-ID: Renee, I've had great results with double labeling using Roche's TUNEL w/ flourescein kit. I just follow the directions using the permeablization solution made with sodium citrate and triton x100. Since this TUNEL kit doesn't actually use an antibody, it is very easy to use for double labels. I actually did some tripples with it if you include DAPI on my frozens. I've done this with FFPE and fresh frozen tissues with good results. I would imagine that it should work fine on cells too. Tom Pier TRIP Lab Department of Pathology UW School of Medicine and Public Health >>> "Till, Renee" 06/20/06 9:58 AM >>> Has anyone tried to co-localize an IHC and Tunel on cells with fluorescence? I saw tons of messages about Tunel, but nothing about this specifically. It seems to me that in some ways cells would present more of a problem. Another tech (I don't do just cells) is trying to do Tunel and Pten fluorescence on the same slide. He has tried doing the Tunel first and then the Pten, and it seemed to work. He is going to try doing it the other way also to compare. You could do multiple slides, but would it be the same as if you were doing tissues that you could use nearly identical sections for each of the stains and the double? What I'm wondering is what are the problems that occur and how does doing fluorescence factor into it. He mentioned not doing the proteinase K step of the Tunel (because it would interfere with the subsequent Pten staining) and still got staining. Is that valid? To just skip a step that you would normally do? He checks the first stain before starting the second, but doesn't take any photos until both are done. Shouldn't you at least take a photo when checking the first stain to see if the second affects it any? Just seems like he has too many variables. Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72002 Office (501)364-2785 Fax (501)364-3161 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rnewlin <@t> burnham.org Tue Jun 20 10:27:19 2006 From: rnewlin <@t> burnham.org (Robbin Newlin) Date: Tue Jun 20 10:27:26 2006 Subject: [Histonet] unsubscribe Message-ID: <1F97379A556D0946AAEFE3F63FD6F5740103ACD3@MAIL.burnham.org> Robbin Newlin HT QIHC ASCP Histology Core Facility Manager The Burnham Institute for Medical Research 10901 N. Torrey Pines Rd. La Jolla, Ca. 92037 858-646-3100 ext. 4249 From rodger_65489 <@t> yahoo.com Tue Jun 20 10:30:58 2006 From: rodger_65489 <@t> yahoo.com (Roger Smithwell) Date: Tue Jun 20 10:31:11 2006 Subject: [Histonet] Microwave user's....do you need extra plastic accessories?? Message-ID: <20060620153058.90591.qmail@web31411.mail.mud.yahoo.com> There is a company out there that makes plastic microwave accessories to fit any unit extremly economical. They custom make accessories to fit your needs. Email them and they will be happy to help you! Coleman_Manufacturing@yahoo.com office phone #: 1-803-633-2124 --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. From Shirley.Chu <@t> moldev.com Tue Jun 20 10:48:19 2006 From: Shirley.Chu <@t> moldev.com (Shirley Chu) Date: Tue Jun 20 10:48:41 2006 Subject: [Histonet] Leica Microscope for Sale Message-ID: <056AFE945F2C1F4292A1F8EBA9B84B5B818A67@MI8NYCMAIL15.Mi8.com> I am listing this for a friend. Please contact Ted Choi directly if you are interested or have any questions. Thanks! Leica DMRE microscope with motorized focus, DIC, fluorescence, cooled CCD imaging and 7 objectives, in excellent condition. Asking price is $18,500. Scope configuration: Leica DMRE base (888033), motor focus base. HC Plan 10X/25 eyepieces (507800) 20X Ph1 (506098) N Plan 2.5X/0.7 (506083) HC Plan Fluotar 10x/.3 (506505) HC Plan Fluotar 20x/.5 (506503) HC Plan Fluotar 40x/.7 (506504) HCX Plan Apo L40X/.8 (506155) HCX Plan Apo 63X/1.32-.6 oil (506081) DIC and Fluorescence illumination (N2.1, A, I3 cubes) LEP HBO100 arc lamp power supply, Trinocular phototube head, Diagnostics Instruments coupler RT SPOT Color Cooled CCD Camera Model 2.2.0/power supply/pci board Photos can be found at www. labx.com ad number 288782 (or just search for "leica dmre") Ted Choi, PhD Predictive Biology, Inc. 505 Coast Blvd La Jolla, CA 92037 main: 858-964-3793 fax: 858-964-0659 tchoi@predictivebio.com From mcauliff <@t> umdnj.edu Tue Jun 20 10:59:26 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Jun 20 10:58:57 2006 Subject: [Histonet] histo question In-Reply-To: <8D80EE169A64FA448EBEDC5812BA7F9B1E8D08@UMR-CMAIL1.umr.edu> References: <8D80EE169A64FA448EBEDC5812BA7F9B1E8D08@UMR-CMAIL1.umr.edu> Message-ID: <44981B5E.3070300@umdnj.edu> I would think that a Masson's or Mallory's trichrome would do the trick. Since most protocols are for human/mammalian tissue you will have to fiddle with times. Also, fixation will have an influence on how well the stains 'take' so if the tissue is fixed in formalin an overnight soak in Bouin's (or just the picric acid part) may be necessary. Geoff Campbell, John Thomas (UMR-Student) wrote: >I am a grad student at the University of Missouri Rolla and I am working on 3D reconstructions of amphibians. We are trying to slice and stain some specimans that we have so we can do the reconstructions. I need to be able to dye the muscle and bone different colors and if possible stain the cartilage too, but this is not neccisary. Does anyone have any suggestions as to what technique I should use? > >Thanks >John > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From eshields <@t> bhset.org Tue Jun 20 11:00:09 2006 From: eshields <@t> bhset.org (E Sharon Shields) Date: Tue Jun 20 11:03:10 2006 Subject: [Histonet] Freeze stick or freeze core bx Message-ID: Histonetters, Has any one done any ICH on breast biopsies that where obtained by the freeze stick or freeze core needles? Or has anyone ever heard of the biopsy method? I really need information on this method of breast biopsy. Thank You Sharon Shields CT(ASCP)QIHC Baptist Hospital of East TN Knoxville,TN 856 549-4351 fax 865 632-5316 From Charles.Embrey <@t> carle.com Tue Jun 20 11:31:47 2006 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Tue Jun 20 11:32:05 2006 Subject: [Histonet] Microwave user's....do you need extra plasticaccessories?? Message-ID: I thought unsolicited vendor advertising was taboo on histonet. I get enough spam already. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roger Smithwell Sent: Tuesday, June 20, 2006 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Microwave user's....do you need extra plasticaccessories?? There is a company out there that makes plastic microwave accessories to fit any unit extremly economical. They custom make accessories to fit your needs. Email them and they will be happy to help you! Coleman_Manufacturing@yahoo.com office phone #: 1-803-633-2124 --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kcai <@t> prosci-inc.com Tue Jun 20 11:46:03 2006 From: kcai <@t> prosci-inc.com (karen Cai) Date: Tue Jun 20 11:39:58 2006 Subject: [Histonet] High profile microtome blade Message-ID: <000001c69489$17388bd0$7d01a8c0@prosci.com> Hello, I just saw several messages regarding high profile microtome blades. I am using accuedge high profile blade and want to switch into blades from CL Sturkey. Any modification for the microtome, i.e. angle needed? How to tell the sections are good or the problem of sections is from microtome blades or tissue itself? Thanks in advance, Best Regrads, Karen -- No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.9.1/369 - Release Date: 6/19/2006 From settembr <@t> umdnj.edu Tue Jun 20 12:05:03 2006 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Tue Jun 20 12:06:05 2006 Subject: [Histonet] IHC with p27? Message-ID: Hello Eva, I use Dako's p27 Kip 1 and I use it at a dilution of 1:80 with a tonsil control using Dako's TRS solution in a steamer for 40 minutes. It looks good. Good Luck. Dana Settembre University Hospital - UMDNJ Newark, NJ >>> Eva Andersson 06/20/06 9:31 AM >>> Good morning, I want to do some IHC with a Kip1/p27 antibody from BD Transduction laboratories (610241) but I can't find any references to a working concentration. Has anybody used this antibody for IHC? What conditions did you use? Thanks for all your help, Eva Andersson Research Assistant Georgetown University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DayDawning <@t> wideopenwest.com Tue Jun 20 12:39:08 2006 From: DayDawning <@t> wideopenwest.com (DayDawning) Date: Tue Jun 20 12:41:21 2006 Subject: [Histonet] DETROIT ZOO Message-ID: <002e01c69490$6f8d71e0$6601a8c0@TRUSCOTT> Jackie The Detroit Zoo is actually in Royal Oak, a very nice, and safe subdivision of Detroit. (Detroit gets a really bad rap, you know?) Good places to search for Hotels: Troy, MI-- Awesome mall nearby, Somerset Madison Heights- right off 14 Mile Rd there is a Fairfield Inn and some more shopping at Oakland Mall - not as great as Somerset. Novi- more great shopping and about 20/30 minutes from the Zoo. Make sure they visit downtown Royal Oak - sort of eclectic, and downtown Birmingham- high end shopping. Feel free to contact me personally, Dawn Truscott From dpahisto <@t> yahoo.com Tue Jun 20 13:04:00 2006 From: dpahisto <@t> yahoo.com (Cindy DuBois) Date: Tue Jun 20 13:04:06 2006 Subject: [Histonet] Sentinal Nodes Message-ID: <20060620180400.42911.qmail@web33415.mail.mud.yahoo.com> Does anyone have a protocol for sentinal nodes, particularly how you handle them when received and how you dispose of them? Any help would be greatly appreciated. I can provide my fax# if you would prefer to fax a reply. Cindy DuBois, HT Integrated Pathology Services Stockton Ca --------------------------------- Yahoo! Groups gets better. Check out the new email design. Plus there?s much more to come. From Heather.A.Harper <@t> pcola.med.navy.mil Tue Jun 20 13:15:08 2006 From: Heather.A.Harper <@t> pcola.med.navy.mil (Heather.A.Harper@pcola.med.navy.mil) Date: Tue Jun 20 13:21:11 2006 Subject: [Histonet] High Profile Micrtome Blades Message-ID: I want to thank everybody who sent me their samples and left over high profile blades. Now I have another dilemma. I'm having so much fun trying out new blades. I want to know if anybody can help me trouble shoot this problem in cutting. When I tried the Duraedge and Sturkey blades, I noticed, one section would be thick, the next was thin. With the AccuEdge, it cut nice except the ribbons were wrinkled and I had to stretched them out on the water bath. It's almost as though the blade is too thick or I need something adjusted on the knife holder. I cut at 5 degree angle and always have. Anybody have any ideas on what to do here. I am not getting those nice smooth wrinkle free ribbons. I'm concluding it's the blade holder needs adjusting or maybe the angle. Any input would be great. Thanks again everybody. P.S. This is a fairly new microtome. It's less than a year old. Heather A. Harper Histology Supervisor Naval Hospital Pensacola, FL From christahercher <@t> yahoo.ca Tue Jun 20 13:43:05 2006 From: christahercher <@t> yahoo.ca (christa hercher) Date: Tue Jun 20 13:43:12 2006 Subject: [Histonet] golgi and nissl stain Message-ID: <20060620184305.52738.qmail@web36815.mail.mud.yahoo.com> Hello, I am a masters student and have been trying to stain frozen human brain tissue with a variation of the golgi-Kopsch method along with a nissl stain. Briefly, a tissue block is first being fixed and then is completely stained with golgi. Then we do the nissl stain on these sections. I have a few questions regarding both stains: The golgi stain works alright but there are alot of black "blobs" in the tissue. Is there something we can do to avoid these clumps? we are also having trouble counterstaining with nissl. The best result so far has been omitting acetic acid in 70% ETOH during the differentiation process. I am just doing the nissl stain to view the cortical layers. If anyone has any suggestions it would be much appreciated thank you christa masters student McGill University, Montreal --------------------------------- Now you can have a huge leap forward in email: get the new Yahoo! Mail. From RSRICHMOND <@t> aol.com Tue Jun 20 14:48:58 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Tue Jun 20 14:49:12 2006 Subject: [Histonet] Re: Freezing small pieces of skeletal muscle tissue Message-ID: <53d.30375b.31c9ab2a@aol.com> Sarah E. Lewis, Histotechnician at Mendell Neuromuscular Lab, Childrens Research Center for Gene Therapy in Columbus OH notes: >>This is the protocol we send to outside institutions... You mentioned you wait until the 2-methylbutane / isopentane gets slushy... This we do not do. I listen for the "boiling" sound to stop (so to speak). Your solution should be clear, not cloudy. You will start to see little white crystals form... This is when you put your tissue in the 2-methylbutane suspended in liquid nitrogen. A 0.5x0.5x1.0 cm piece of muscle should be in for 30 sec. Tissue any smaller, cut your time down by 5 sec according to size. Hold tissue in cryostat or -20/-40 until tissue is dry. Wrap with foil and store in -80.<< Many people cover the specimen with a thin layer of talcum powder before dipping it int the 2-MB. Also, it helps to dip the specimen up and down at about 1 second intervals for at least 10 dips. - These strategies avoid the formation of large ice crystals ("waffle artifact") within muscle fibers. I'd suggest trying this with some practice muscle - from an amputated leg or a researcher's left-over dead rat, depending on where you are - before freezing a patient specimen - cut frozen sections to see if you have artifact. 2MB (like acetone) is a fire hazard. A few weeks ago I posted a note about using 3M's HFE-7100 - methyl nonafluoroisobutyl ether C4F9-O-CH3 instead. Does anyone know about using this stuff as a non-flammable freeze medium? Bob Richmond Gastonia NC From amosbrooks <@t> gmail.com Tue Jun 20 17:38:51 2006 From: amosbrooks <@t> gmail.com (Amos Brooks) Date: Tue Jun 20 17:38:56 2006 Subject: [Histonet] Re: Histonet Digest, Vol 31, Issue 28 In-Reply-To: <44981c41.76d7ece0.62b9.5953SMTPIN_ADDED@mx.gmail.com> References: <44981c41.76d7ece0.62b9.5953SMTPIN_ADDED@mx.gmail.com> Message-ID: <582736990606201538t40fac148l73eef539c9376858@mail.gmail.com> Renee, Our lab has been looking at the Sakura Rapid Tissue Processor. As we haven't implimented it for use yet, I can't really make any claims for or against the processor. I will say however that they emphasize loudly the need for the tissue to be trimmed REALLY thin (by way of comparason to what our residents are currently doing). They even have a tool to regulate the tissue thickness. I have been saying that if they could trim the tissue this thin with routine processing they would have far less taken-back tissue and the processing would be quicker. My point is that regaurdless of what fixative you use or weather it is microwaved or not, grossing into thin sections is imperative. The fixative contact time is a function of the thickness of the tissue, as I'm sure you know. You might want to suggest thinner grossing as they will end up having to do this anyway if you end up with a microwave processor. Sincerely, Amos Brooks Message: 12 > Date: Tue, 20 Jun 2006 08:15:02 -0400 > From: "RENEE FISHER" > Subject: [Histonet] Hello all, our lab is thinking about getting the > microwave processor for bx's and reprocessing tis > To: > Message-ID: <4497AE86020000CA00002372@mail.gbmc.org> > Content-Type: text/plain; charset=US-ASCII > > Hello all, our lab is thinking about getting the microwave processor > for bx's and reprocessing tissue. I wanted to get some feed back from > those of you who have the MW processor, please feel free to e-mail me > your thoughts, also we reprocess a great number of blocks ( fatty breast > that has been pushed through for the 24hr turn around) wanted some > thoughts on running tissue back to formalin sometimes when we do this > the tissue is hard and brittle. > > Thanks, Renee From caramel-bonbon <@t> caramail.com Tue Jun 20 18:02:33 2006 From: caramel-bonbon <@t> caramail.com (HistoHopeful) Date: Tue Jun 20 18:35:01 2006 Subject: [Histonet] Here's to hope... Message-ID: <17638442052823@lycos-europe.com> Greetings All, Recently there were a few postings on the subject of "online histology programs" and the Darton college came up. I went to the website ( http://www.darton.edu/programs/AlliedHealth/hist/index.php ) but I could not find any information about online options for their program. Could anybody supply further information on this possibilitiy? My current situation is that I just completed a two-term "introduction to histologic techniques" through a community college in my area. There is not an accredited one or two year program leading directly to certification in my whole state or I would have certainly considered taking that route. The program that I did go through does qualify me to take the ASCP exam after a year of on-the-job experience however. The dilemma I seem to be facing though is that most of the places in my metro area are looking of already seasoned histotechs with certification. Openings are coming up so infrequently in my area that I am having to start thinking about moving to get experience under my belt. If I have to move, I was thinking that perhaps there might be some international aid organizations (NGO?) or other means of volunteer or paid service outside the US that I could also consider. If anybody has leads on the above idea or encouragement for me I would sure appreciate it. I am somewhat worried that the rather expensive program I went through was a bit of a mirage on employability after completion. Lycos iQ : Posez votre question maintenant et obtenez des r?ponses d?Experts. Cliquez ici ! From lpwenk <@t> sbcglobal.net Tue Jun 20 19:28:53 2006 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Tue Jun 20 19:29:08 2006 Subject: [Histonet] Here's to hope... In-Reply-To: <17638442052823@lycos-europe.com> Message-ID: <002701c694c9$ad0e4a10$c7022643@HPPav2> My name is Peggy Wenk, and I'm the program director of both a HT and a HTL program (NAACLS accredited) in Royal Oak, Michigan. I don't know of any accredited HT programs that are totally on-line. Some of the college based programs have SOME of their lectures on line, but students still need to go to the college to take the labs for the courses. In addition, students need to do their histology rotations at affiliated hospitals that are usually in the area. This is a very much hands-on profession. Being totally on-line does not provide the hands-on experience. You could contact NAACLS (National Accrediting Agency for Clinical Laboratory Sciences), which accredits HT programs. 773-714-8880 They would know what programs have on-line courses. I do have some concerns about the Intro to Histologic Techniques courses you took at your local community college. You say that the "program . . . does qualify me to take the ASCP exam after a year of on-the-job experience." Actually, ASCP says that anyone having 1 year OJT experience in histology at any histology lab qualifies them to take the ASCP HT exam. They do NOT have to first take a 2 term into to histologic technique course. I hope the college didn't lead you to believe that this course that you had to pay for was required to take the exam (with the 1 year experience). Second area of concern - the new ASCP requirement for those qualifying for the HT exam via the OJT route is that the candidate must have an associate degree or 60 semester hours minimum with at least 20 credits of biology and chemistry combined AND the 1 year OJT. If all you took was these two courses, you would not be able to take the exam. What exactly is this college telling people about qualifying for the HT exam? I really have concerns. Who is this college? Where are they? Now, on to your suggestion about going outside the US. There is a problem with this, if your goal is to qualify to take the ASCP registry exam. The requirement for the experience route is that the experience be under a "pathologist certified by the American Board of Pathology in Anatomic Pathology, or an appropriately board certified medical scientist". In otherwords, that pathologist must be US Board certified, and the medical scientist (e.g., veterinary pathology) must also be US Board certified. So working under a pathologist certified in another country would not qualify you to take the ASCP HT exam. If however, you just want to gain experience so that you can come back to the US and possibly get a HT job more easily, and then get the 1 year OJT in the US hospital, then you would meet the requirement to take the HT exam. http://ascp.org/Certification/CertifyingExaminations/cert_procedures/eligibi lity/ht.aspx Again, I would suggest you contact NAACLS. Peggy A. Wenk, HTL(ASCP) William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of HistoHopeful Sent: Tuesday, June 20, 2006 2:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Here's to hope... Greetings All, Recently there were a few postings on the subject of "online histology programs" and the Darton college came up. I went to the website ( http://www.darton.edu/programs/AlliedHealth/hist/index.php ) but I could not find any information about online options for their program. Could anybody supply further information on this possibilitiy? My current situation is that I just completed a two-term "introduction to histologic techniques" through a community college in my area. There is not an accredited one or two year program leading directly to certification in my whole state or I would have certainly considered taking that route. The program that I did go through does qualify me to take the ASCP exam after a year of on-the-job experience however. The dilemma I seem to be facing though is that most of the places in my metro area are looking of already seasoned histotechs with certification. Openings are coming up so infrequently in my area that I am having to start thinking about moving to get experience under my belt. If I have to move, I was thinking that perhaps there might be some international aid organizations (NGO?) or other means of volunteer or paid service outside the US that I could also consider. If anybody has leads on the above idea or encouragement for me I would sure appreciate it. I am somewhat worried that the rather expensive program I went through was a bit of a mirage on employability after completion. Lycos iQ : Posez votre question maintenant et obtenez des r?ponses d?Experts. Cliquez ici ! From jcox90 <@t> yahoo.com Tue Jun 20 20:40:17 2006 From: jcox90 <@t> yahoo.com (Jill Cox) Date: Tue Jun 20 20:40:21 2006 Subject: [Histonet] Used Microtomes for sale in Phoenix AZ Message-ID: <20060621014017.86092.qmail@web52105.mail.yahoo.com> Hi Histonetters, I have two used microtomes I am selling in Phoenix AZ. One is an Olympus Cut 4060 and the other is a Leica 2030. Please call for details if interested, Thanks Jill 602-481-1424 Jill Cox HT (ASCP) From POWELL_SA <@t> Mercer.edu Tue Jun 20 22:35:22 2006 From: POWELL_SA <@t> Mercer.edu (powell_sa) Date: Tue Jun 20 22:27:39 2006 Subject: [Histonet] Here's to hope... Message-ID: <4498D8E6@webmail.mercer.edu> Go to wwww.darton.edu, click on Allied health at the top, click on online degrees at the bottom of the left column, then click on the drop down box at the top where it says "Choose your degree" and you will find the histology courses. Shirley Powell >===== Original Message From HistoHopeful ===== >Greetings All, Recently there were a few postings on the subject of "online histology programs" and the Darton college came up. I went to the website ( http://www.darton.edu/programs/AlliedHealth/hist/index.php ) but I could not find any information about online options for their program. Could anybody supply further information on this possibilitiy? My current situation is that I just completed a two-term "introduction to histologic techniques" through a community college in my area. There is not an accredited one or two year program leading directly to certification in my whole state or I would have certainly considered taking that route. The program that I did go through does qualify me to take the ASCP exam after a year of on-the-job experience however. The dilemma I seem to be facing though is that most of the places in my metro area are looking of already seasoned histotechs with certification. Openings are coming up so infrequently in my area that I am having to start thinking about moving to get experience under my belt. If I have to move, I was thinking that perhaps there might be some international aid organizations (NGO?) or other means of volunteer or paid service outside the US that I could also consider. If anybody has leads on the above idea or encouragement for me I would sure appreciate it. I am somewhat worried that the rather expensive program I went through was a bit of a mirage on employability after completion. Lycos iQ : Posez votre question maintenant et obtenez des r?ponses d?Experts. Cliquez ici ! > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brucea <@t> unimelb.edu.au Wed Jun 21 01:58:08 2006 From: brucea <@t> unimelb.edu.au (Bruce Abaloz) Date: Wed Jun 21 01:58:28 2006 Subject: [Histonet] abalone phagocytes Message-ID: Diffferentiation of zymosan from phagocytes Hi, I have stained some abalone phagocytes (abalone haemocytes are like mammalian macrophages) which have phagocytosed zymosan. Stain is wrights giemsa. I can easily see the extracellular zymosan but not the Intracellular zymosan. Also not clear when a zymosan is inside versus stuck on the cell outside. Bruce, our Histologist tells me this is a wonderful 'tool' for exchange of information & I AM hoping there is someone who can advise me of a 'better' stain which will allow me to differentiate/see the intracellular zymosan. Thanking you in advance, Celia -- BRUCE ABALOZ PH:61383446282 HISTOLOGIST FAX:61383447909 DEPT. of ZOOLOGY EMAIL: brucea@unimelb.edu.au THE UNIVERSITY Of MELBOURNE. VICTORIA. AUSTRALIA 3010 Nobody Can Make You Feel Inferior Without YOUR Permission - Eleanor Roosevelt DANCE LIKE NO-ONE'S WATCHING Q: What's a specimen? A: An Italian astronaut. From cbass <@t> bidmc.harvard.edu Wed Jun 21 02:03:01 2006 From: cbass <@t> bidmc.harvard.edu (Caroline Bass) Date: Wed Jun 21 02:03:09 2006 Subject: [Histonet] to perfuse or not to perfuse... Message-ID: <8DD12000-ABCF-4BEC-BC63-D3FA53ABD62C@bidmc.harvard.edu> I hate to broach such a seemingly banal question, but I searched the archives and couldn't find exactly what I need. So here goes... I have been using a virus to express EGFP in mice brains with great results. I perfuse the mice, post-fix overnight, immerse in 25% sucrose overnight, section at 40 microns, mount and visualize the native fluorescence. Now I want to send my virus to another lab to test, however, they don't know how to perfuse. Do you think the native fluorescence will be retained if the brain is collected fresh and immersed in NBF? Are there any suggestions for preparing the brain other than by perfusion? Thanks! From Mary.Judd <@t> suht.swest.nhs.uk Wed Jun 21 05:31:23 2006 From: Mary.Judd <@t> suht.swest.nhs.uk (Mary Judd) Date: Wed Jun 21 05:31:49 2006 Subject: [Histonet] MLH-1 Message-ID: We use MLH-1(14) from Zymed cat.no. 18-2342 We pressure cook in citrate buffer 6pH, use the antibody at 1:30 and incubate overnight at 4C. Mary Judd Section Head Immunohistochemistry Cellular Pathology Level E, Mailpoint 2 Southampton University Hospitals Trust Tel. 02380 795144 Fax. 02380 796869 D I S C L A I M E R This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Trust unless explicitly stated otherwise. If you have received this e-mail in error please delete the e-mail and contact the Southampton University Hospitals NHS Trust Helpdesk on:- 023 80796000 The information contained in this e-mail may be subject to public disclosure under the Freedom of Information Act 2000. Unless the Information is legally exempt from disclosure, the confidentiality of this e-mail and your reply cannot be guaranteed. This footnote also confirms that this email message has been swept by MailMarshal for the presence of computer viruses. Please visit our website at http://www.suht.nhs.uk From esther.peters <@t> verizon.net Wed Jun 21 07:12:33 2006 From: esther.peters <@t> verizon.net (Esther Peters) Date: Wed Jun 21 07:12:38 2006 Subject: [Histonet] fixing sponge larvae for in situs References: <464184F7-0FAF-4655-AB73-F842D6B22E76@richmond.edu> Message-ID: <449937B1.70303@verizon.net> Dear Gary, Is your colleague working on marine or freshwater sponge larvae? The osmolarity of the fixative, as someone else on the list mentioned, is key. Even 1-2% PFA in seawater might adversely affect the larvae. It would be best to check the osmolarity of the fixative using an osmometer to get it as close as possible to the seawater they are in (or freshwater, if that is the case). I have fixed corals for years and we have some "unknowns" in some of the sections that we suspect are marine sponge larvae. These were fixed using Helly's (potassium dichromate, zinc chloride, and formaldehyde). They are just 1 mm or less round to ovoid aggregations of cells, not much CT (spongin) at all, so might be very delicate to handle in a water sample and might be "exploding" because of the way they are handled post-fixation (how are they removed from the fixative and processed)? We are now using Z-Fix concentrate diluted 1 part to 4 parts filtered seawater for our coral fixations. This might be okay for the sponge larvae? We also use this fixative, as well as others, on coral larvae with excellent results and have never had to anesthetize them before fixation (as Rene had suggested). But the epithelial cells in coral (and other cnidarian planulae (term for these larvae) are anchored to a layer of primitive connective tissue, or mesoglea, within 24 hours or so of fertilization. Would be interested in learning if you find a successful fixation protocol! Esther Peters, Ph.D. George Mason University Gary Radice wrote: > I have a colleague who wants to do in situ hybridizations on sponge > larvae (yes, sponges are animals and they have larval forms!). She > tried fixing some in 4% paraformaldehyde in sea water, and the larvae > exploded. She also tried Carnoy's fix, and 4% PFA in Millonig's > phosphate buffer, and the same thing happened. > > The only thing I could think of to suggest was dropping to 1 or 2% PFA > in sea water. I've seen references to using Bouins for light microscopy > or osmium tetroxide for TEM, but I don't think either of these > approaches would be good for in situs. > > Any other ideas? > > > > > Gary P. Radice gradice@richmond.edu > Department of Biology 804-289-8107 (voice) > University of Richmond 804-289-8233 (FAX) > Richmond VA 23173 http://www.richmond.edu/~gradice > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Traczyk7 <@t> aol.com Wed Jun 21 07:53:44 2006 From: Traczyk7 <@t> aol.com (Traczyk7@aol.com) Date: Wed Jun 21 07:53:55 2006 Subject: [Histonet] MW accessories Message-ID: <527.657444.31ca9b58@aol.com> I am trying to help one of my customers get and Energy Beam MW, bought from Ebay (Yuk) that did not come with any accessories, go figure. I would like to speak with anyone who has ordered mw accessories from the Coleman's about quality and order process. Did you send them a piece to copy or maybe a picture? Did they make something customized to your specifications? How did the price compare to what you could get directly from the original vendor? Please contact me directly at dorothy@hackerinstruments.com. Thanks to all. Dorothy Murphy Traczyk National Sales Manager Hacker Instruments & Industries Inc. PO Box 1176 Winnsboro, SC 29180 800-442-2537 From chiggerson <@t> memhosp.com Wed Jun 21 08:06:55 2006 From: chiggerson <@t> memhosp.com (chiggerson@memhosp.com) Date: Wed Jun 21 08:07:07 2006 Subject: [Histonet] Same Day Breast Biopsy Results Message-ID: Histonetters, I need information on providing same day results on breast biopsy specimens. Is anyone out there doing this??? We have a surgeon on staff at our institution that is demanding same day rush breast biopsy results. We often rush other types of biopsies and provide same day results, but for obvious reasons (fatty, Her2 fixation, etc.) we do not provide this service for breast tissue. I do provide first am results. He has insisted that other institutions are providing same day breast results and is not happy with waiting till 8 am the next morning. I called other labs in our area and they are not doing same day breast results for the same reasons we are not. Can anyone help me with this? Your help is greatly appreciated! Thanks, Cindy From JWEEMS <@t> sjha.org Wed Jun 21 08:21:33 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Jun 21 08:21:53 2006 Subject: [Histonet] Same Day Breast Biopsy Results Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202960028@sjhaexc02.sjha.org> I would provide him with the FDA requirements. Surely he would not want to compromise the treatment of his patient. Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of chiggerson@memhosp.com Sent: Wednesday, June 21, 2006 9:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Same Day Breast Biopsy Results Histonetters, I need information on providing same day results on breast biopsy specimens. Is anyone out there doing this??? We have a surgeon on staff at our institution that is demanding same day rush breast biopsy results. We often rush other types of biopsies and provide same day results, but for obvious reasons (fatty, Her2 fixation, etc.) we do not provide this service for breast tissue. I do provide first am results. He has insisted that other institutions are providing same day breast results and is not happy with waiting till 8 am the next morning. I called other labs in our area and they are not doing same day breast results for the same reasons we are not. Can anyone help me with this? Your help is greatly appreciated! Thanks, Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From Charles.Embrey <@t> carle.com Wed Jun 21 08:38:51 2006 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Wed Jun 21 08:39:00 2006 Subject: [Histonet] Same Day Breast Biopsy Results Message-ID: In my opinion what your surgeon is asking for is detrimental to proper patient care, results in substandard slides and opens both the hospital and pathologist to potential malpractice. Breast, because of its fatty nature is slower to fix and process than other tissues and rush processing usually results in poor slides. A recent post on histonet stated that they have to reprocess "a lot of blocks", mainly breast, because of difficulty in sectioning. What if that tissue, wasted in the first attempts at processing contained the tumor and the reprocessed tissue is now tumor free? With today's imaging studies tumors and lesions are being found earlier and thus smaller that ever. You can't afford to waste any tissue in an attempt to rapid process. All this aside, what is the surgeon going to do with same day results? How are same day results going to affect his care of the patient? How is waiting 24 to 48 hours going to have a negative impact on his care for the patient? I suspect, the surgeon only want to look good to the patient by giving them the report quickly. The bottom line is that your Chief Pathologist is going to have to put his foot down and say NO. It is better for the patient to have good histology and a confident diagnosis than speed and a suspect diagnosis. Personally, we fix all breast tissues, even cores, overnight before processing and get great results. You can tell your surgeon that my facility will not jeopardize patient care for speed. Charles Embrey Jr., PA(ASCP) Histology Manager Carle Clinic, Illinois -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of chiggerson@memhosp.com Sent: Wednesday, June 21, 2006 8:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Same Day Breast Biopsy Results Histonetters, I need information on providing same day results on breast biopsy specimens. Is anyone out there doing this??? We have a surgeon on staff at our institution that is demanding same day rush breast biopsy results. We often rush other types of biopsies and provide same day results, but for obvious reasons (fatty, Her2 fixation, etc.) we do not provide this service for breast tissue. I do provide first am results. He has insisted that other institutions are providing same day breast results and is not happy with waiting till 8 am the next morning. I called other labs in our area and they are not doing same day breast results for the same reasons we are not. Can anyone help me with this? Your help is greatly appreciated! Thanks, Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Diane.Gladney <@t> se.amedd.army.mil Wed Jun 21 09:13:04 2006 From: Diane.Gladney <@t> se.amedd.army.mil (Gladney, Diane C Ms MACH) Date: Wed Jun 21 09:14:00 2006 Subject: [Histonet] Same Day Breast Biopsy Results In-Reply-To: Message-ID: <4D55B2E997EFAE4DA6081DDE100B8302EC9246@amedmlsermc133> Charles is completely correct. We do no do same day breast biopsies for the same reasons and for the FDA requirements for ER/PR and Her2Neu analysis. This surgeon is way out in left field and your chief pathologist needs to educate him. I had the same problem here with one of our surgeons and both the chief pathologist and I gave him a short course on fixation and unfixed specimens. I even showed him the difference in the results of poorly fixed vs fixed tissue slides. He was most impressed and has not pressured me to submit unfixed or poorly fixed breast tissue again. Surgeons do not know or understand the techniques and procedures of excellent histology. They have to be gently educated so that they can provide the best patient care. Thanks, Diane Diane C. Gladney, HT (ASCP) Supervisor, Histology Dept. Moncrief Army Community Hospital Dept. of Pathology 4500 Stuart St. FT. Jackson, SC 29207 Email: diane.gladney@se.amedd.army.mil Phone: 803-751-2530 FAX: 803-751-7829 DSN: 734-2530 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles.Embrey Sent: Wednesday, June 21, 2006 9:39 AM To: chiggerson@memhosp.com Cc: Histonet@pathology.swmed.edu Subject: RE: [Histonet] Same Day Breast Biopsy Results In my opinion what your surgeon is asking for is detrimental to proper patient care, results in substandard slides and opens both the hospital and pathologist to potential malpractice. Breast, because of its fatty nature is slower to fix and process than other tissues and rush processing usually results in poor slides. A recent post on histonet stated that they have to reprocess "a lot of blocks", mainly breast, because of difficulty in sectioning. What if that tissue, wasted in the first attempts at processing contained the tumor and the reprocessed tissue is now tumor free? With today's imaging studies tumors and lesions are being found earlier and thus smaller that ever. You can't afford to waste any tissue in an attempt to rapid process. All this aside, what is the surgeon going to do with same day results? How are same day results going to affect his care of the patient? How is waiting 24 to 48 hours going to have a negative impact on his care for the patient? I suspect, the surgeon only want to look good to the patient by giving them the report quickly. The bottom line is that your Chief Pathologist is going to have to put his foot down and say NO. It is better for the patient to have good histology and a confident diagnosis than speed and a suspect diagnosis. Personally, we fix all breast tissues, even cores, overnight before processing and get great results. You can tell your surgeon that my facility will not jeopardize patient care for speed. Charles Embrey Jr., PA(ASCP) Histology Manager Carle Clinic, Illinois -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of chiggerson@memhosp.com Sent: Wednesday, June 21, 2006 8:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Same Day Breast Biopsy Results Histonetters, I need information on providing same day results on breast biopsy specimens. Is anyone out there doing this??? We have a surgeon on staff at our institution that is demanding same day rush breast biopsy results. We often rush other types of biopsies and provide same day results, but for obvious reasons (fatty, Her2 fixation, etc.) we do not provide this service for breast tissue. I do provide first am results. He has insisted that other institutions are providing same day breast results and is not happy with waiting till 8 am the next morning. I called other labs in our area and they are not doing same day breast results for the same reasons we are not. Can anyone help me with this? Your help is greatly appreciated! Thanks, Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rosa_113 <@t> hotmail.com Wed Jun 21 09:47:36 2006 From: rosa_113 <@t> hotmail.com (Rosa Bryant) Date: Wed Jun 21 09:47:40 2006 Subject: [Histonet] control block? Message-ID: Can anyone spare a formaldehyde fixed paraffin embedded control block for Lepto? Any help would be great! Rosa Fields, IHC VDC UNL rosa_113@hotmail.com _________________________________________________________________ Express yourself: design your homepage the way you want it with Live.com. http://www.live.com/getstarted From jtcrhb <@t> umr.edu Wed Jun 21 10:21:52 2006 From: jtcrhb <@t> umr.edu (Campbell, John Thomas (UMR-Student)) Date: Wed Jun 21 10:22:14 2006 Subject: [Histonet] histoclear vs xylene Message-ID: <8D80EE169A64FA448EBEDC5812BA7F9B1E8D0C@UMR-CMAIL1.umr.edu> I have read several protocols on staining slides and most of them talk about using xylene the protocol that I am currently using says to use Histoclear. I was wondering what the difference was and I would like to know the pros and cons on using xylene and using histoclear. what do you all use and what exactly are the hazards of using xylene John Campbell Graduate Student University of Missouri Rolla Lab phone 573-341-4069 Cell phone 573-694-0596 From mauger <@t> email.chop.edu Wed Jun 21 11:05:04 2006 From: mauger <@t> email.chop.edu (Joanne Mauger) Date: Wed Jun 21 11:05:47 2006 Subject: [Histonet] Re:salary for IHC techs Message-ID: Hi everyone, Can anyone share info on whether the techs in your labs who do immunohistochemistry have a different pay scale than those who do histology? Is there a differential made between those who have a Q(IHC)? We are trying to find precedent to give incentive for learning new skills, taking on more responsibility. Any info is appreciated, Jo Mauger >>> "Rosa Bryant" 06/21/06 10:47 AM >>> Can anyone spare a formaldehyde fixed paraffin embedded control block for Lepto? Any help would be great! Rosa Fields, IHC VDC UNL rosa_113@hotmail.com _________________________________________________________________ Express yourself: design your homepage the way you want it with Live.com. http://www.live.com/getstarted_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Jun 21 11:15:59 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 21 11:16:05 2006 Subject: [Histonet] histoclear vs xylene In-Reply-To: <8D80EE169A64FA448EBEDC5812BA7F9B1E8D0C@UMR-CMAIL1.umr.edu> Message-ID: <20060621161559.61096.qmail@web61212.mail.yahoo.com> John: In a "nutshell" I can tell you the following: 1-xylene: universally used for tissue processing and staining but a product that should be avoided because of known health hazards. 2-histoclear: a "xylene substitute" sometimes used as a replacement of xylene. Does not work equally well. It is "allegedly safe" but really with no documentation of long term health effects as xylene has. Maybe with time it will turn out to be as hazardous as xylene. Our profession has some health hazards and we just have to work as safely as possible. Hope this will help you! "Campbell, John Thomas (UMR-Student)" wrote: I have read several protocols on staining slides and most of them talk about using xylene the protocol that I am currently using says to use Histoclear. I was wondering what the difference was and I would like to know the pros and cons on using xylene and using histoclear. what do you all use and what exactly are the hazards of using xylene John Campbell Graduate Student University of Missouri Rolla Lab phone 573-341-4069 Cell phone 573-694-0596 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Next-gen email? Have it all with the all-new Yahoo! Mail Beta. From rjbuesa <@t> yahoo.com Wed Jun 21 11:22:09 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 21 11:22:14 2006 Subject: [Histonet] Re:salary for IHC techs In-Reply-To: Message-ID: <20060621162209.11712.qmail@web61213.mail.yahoo.com> Joanne: In the 3 labs I supervised before retiring (and some gathered information from other sources) doing IHC did not qualify for a higger salary per se but that was a task usually assigned to HTL and not to HT, meaning that they were in a higher paying scale anyway. As a rule of thumb I could tell you the following: 1- unlicensed tasks done by lab aides: average of $15/hour 2- licensed tasks by HT (histotechs): average $22/hour 3- licensed tasks by HTL (histotechnologists): average $25/hour. Consider that averages are close to "mid points" ranges due to specific institutions pay rates. Hope this will help you! Ren? J. Joanne Mauger wrote: Hi everyone, Can anyone share info on whether the techs in your labs who do immunohistochemistry have a different pay scale than those who do histology? Is there a differential made between those who have a Q(IHC)? We are trying to find precedent to give incentive for learning new skills, taking on more responsibility. Any info is appreciated, Jo Mauger >>> "Rosa Bryant" 06/21/06 10:47 AM >>> Can anyone spare a formaldehyde fixed paraffin embedded control block for Lepto? Any help would be great! Rosa Fields, IHC VDC UNL rosa_113@hotmail.com _________________________________________________________________ Express yourself: design your homepage the way you want it with Live.com. http://www.live.com/getstarted_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1¢/min. From petepath <@t> yahoo.com Wed Jun 21 11:33:45 2006 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Wed Jun 21 11:33:55 2006 Subject: [Histonet] Histonet] Same Day Breast Biopsy Results Message-ID: <20060621163345.41919.qmail@web30403.mail.mud.yahoo.com> I fully agree with Charles. I will add one bit of advice to keep in mind. You will make most of your mistakes when you are being rushed or interrupted. That goes for cutting yourself grossing or at the microtome. So when you are cutting, and someone comes up behind you to push or distract you, put down the scalpel or get away from the microtome blade. When a surgeon rushes me in the OR I tell him he made me nervous and now I have to go to the bathroom and will take another ten minutes. If somebody tries to rush a process that is already being done at the correct speed, ask them if they would like a fast answer, or whether they are willing to wait for the correct answer. They will get the message. Thanks Charles. Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com From chiggerson <@t> memhosp.com Wed Jun 21 11:46:14 2006 From: chiggerson <@t> memhosp.com (chiggerson@memhosp.com) Date: Wed Jun 21 11:46:24 2006 Subject: [Histonet] Same Day Breast Biopsy Results In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD3202960028@sjhaexc02.sjha.org> Message-ID: Thank you for your responses! I have had three run-ins with this particular surgeon and neither of us is willing to budge. Thank you for the affirmation that I am not the one out in left field................... I plan to stand my ground and will not compromise patient care. Thanks! Cindy Charles is completely correct. We do no do same day breast biopsies for the same reasons and for the FDA requirements for ER/PR and Her2Neu analysis. This surgeon is way out in left field and your chief pathologist needs to educate him. I had the same problem here with one of our surgeons and both the chief pathologist and I gave him a short course on fixation and unfixed specimens. I even showed him the difference in the results of poorly fixed vs fixed tissue slides. He was most impressed and has not pressured me to submit unfixed or poorly fixed breast tissue again. Surgeons do not know or understand the techniques and procedures of excellent histology. They have to be gently educated so that they can provide the best patient care. Thanks, Diane Diane C. Gladney, HT (ASCP) Supervisor, Histology Dept. Moncrief Army Community Hospital Dept. of Pathology 4500 Stuart St. FT. Jackson, SC 29207 In my opinion what your surgeon is asking for is detrimental to proper patient care, results in substandard slides and opens both the hospital and pathologist to potential malpractice. Breast, because of its fatty nature is slower to fix and process than other tissues and rush processing usually results in poor slides. A recent post on histonet stated that they have to reprocess "a lot of blocks", mainly breast, because of difficulty in sectioning. What if that tissue, wasted in the first attempts at processing contained the tumor and the reprocessed tissue is now tumor free? With today's imaging studies tumors and lesions are being found earlier and thus smaller that ever. You can't afford to waste any tissue in an attempt to rapid process. All this aside, what is the surgeon going to do with same day results? How are same day results going to affect his care of the patient? How is waiting 24 to 48 hours going to have a negative impact on his care for the patient? I suspect, the surgeon only want to look good to the patient by giving them the report quickly. The bottom line is that your Chief Pathologist is going to have to put his foot down and say NO. It is better for the patient to have good histology and a confident diagnosis than speed and a suspect diagnosis. Personally, we fix all breast tissues, even cores, overnight before processing and get great results. You can tell your surgeon that my facility will not jeopardize patient care for speed. Charles Embrey Jr., PA(ASCP) Histology Manager Carle Clinic, Illinois I would provide him with the FDA requirements. Surely he would not want to compromise the treatment of his patient. Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Same Day Breast Biopsy Results Histonetters, I need information on providing same day results on breast biopsy specimens. Is anyone out there doing this??? We have a surgeon on staff at our institution that is demanding same day rush breast biopsy results. We often rush other types of biopsies and provide same day results, but for obvious reasons (fatty, Her2 fixation, etc.) we do not provide this service for breast tissue. I do provide first am results. He has insisted that other institutions are providing same day breast results and is not happy with waiting till 8 am the next morning. I called other labs in our area and they are not doing same day breast results for the same reasons we are not. Can anyone help me with this? Your help is greatly appreciated! Thanks, Cindy From Heather.A.Harper <@t> pcola.med.navy.mil Wed Jun 21 11:41:23 2006 From: Heather.A.Harper <@t> pcola.med.navy.mil (Heather.A.Harper@pcola.med.navy.mil) Date: Wed Jun 21 11:47:15 2006 Subject: [Histonet] Adjusting the angle Message-ID: Well I adjusted the angle like every one suggested, starting in increments. I could not find one angle that Accu Edge blade would cut a smooth section. Every time it was compressed with wrinkles. I tried the Dura Edge and found an angle for that but only had one blade of that left and am out of the edge right. Is Accu Edge this hard to get an angle where it cuts right? I have an RM 2235 Leica microtome. All of a sudden cutting seems to be a nightmare. Heather A. Harper Naval Hospital Pensacola, FL From rjbuesa <@t> yahoo.com Wed Jun 21 12:09:03 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 21 12:09:09 2006 Subject: [Histonet] Adjusting the angle In-Reply-To: Message-ID: <20060621170903.82027.qmail@web61212.mail.yahoo.com> Heather: Have you consider checking the working condition of your microtome? Sometimes all those problems stem form a "need to adjust" microtome. We always used AccuEdge and never changed to any other brand even when we received free samples to "temp us". Hope this will help you! Ren? J. Heather.A.Harper@pcola.med.navy.mil wrote: Well I adjusted the angle like every one suggested, starting in increments. I could not find one angle that Accu Edge blade would cut a smooth section. Every time it was compressed with wrinkles. I tried the Dura Edge and found an angle for that but only had one blade of that left and am out of the edge right. Is Accu Edge this hard to get an angle where it cuts right? I have an RM 2235 Leica microtome. All of a sudden cutting seems to be a nightmare. Heather A. Harper Naval Hospital Pensacola, FL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. From corycody <@t> msn.com Wed Jun 21 12:12:41 2006 From: corycody <@t> msn.com (Bly Haverland) Date: Wed Jun 21 12:12:49 2006 Subject: [Histonet] used histology equipment for sale Message-ID: I have several pieces of older histology equipment for sale including a Tissue Tek embedding center, an older Leica microtome, a linear stainer, water baths, embedding molds, and miscellaneous glassware. If interested, please contact Bly @ # (919) 279-8371 or e-mail at corycody@msn.com Best offers will be taken and shipping needs to be paid for by buyer. Equipment is located in Raleigh, NC. Thank you. From rjbuesa <@t> yahoo.com Wed Jun 21 12:22:56 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 21 12:23:00 2006 Subject: [Histonet] Same Day Breast Biopsy Results In-Reply-To: Message-ID: <20060621172256.45242.qmail@web61214.mail.yahoo.com> Dear Cindy: Let me try to contribute with my "2 cents": It seems to me that there is more to it than the just "same day" breast biospy issue. This can be done and is done daily at labs with the new Sakura Throughput tissue processing technology. It is done in less than 4 hours and there is no limit to IHC tests. This new technology is not reflected in the regulations that have been mentioned but rest assured that the pathologists working in labs with that technology are not going to compromise a diagnostic for speed sake. Ren? J. chiggerson@memhosp.com wrote: Thank you for your responses! I have had three run-ins with this particular surgeon and neither of us is willing to budge. Thank you for the affirmation that I am not the one out in left field................... I plan to stand my ground and will not compromise patient care. Thanks! Cindy Charles is completely correct. We do no do same day breast biopsies for the same reasons and for the FDA requirements for ER/PR and Her2Neu analysis. This surgeon is way out in left field and your chief pathologist needs to educate him. I had the same problem here with one of our surgeons and both the chief pathologist and I gave him a short course on fixation and unfixed specimens. I even showed him the difference in the results of poorly fixed vs fixed tissue slides. He was most impressed and has not pressured me to submit unfixed or poorly fixed breast tissue again. Surgeons do not know or understand the techniques and procedures of excellent histology. They have to be gently educated so that they can provide the best patient care. Thanks, Diane Diane C. Gladney, HT (ASCP) Supervisor, Histology Dept. Moncrief Army Community Hospital Dept. of Pathology 4500 Stuart St. FT. Jackson, SC 29207 In my opinion what your surgeon is asking for is detrimental to proper patient care, results in substandard slides and opens both the hospital and pathologist to potential malpractice. Breast, because of its fatty nature is slower to fix and process than other tissues and rush processing usually results in poor slides. A recent post on histonet stated that they have to reprocess "a lot of blocks", mainly breast, because of difficulty in sectioning. What if that tissue, wasted in the first attempts at processing contained the tumor and the reprocessed tissue is now tumor free? With today's imaging studies tumors and lesions are being found earlier and thus smaller that ever. You can't afford to waste any tissue in an attempt to rapid process. All this aside, what is the surgeon going to do with same day results? How are same day results going to affect his care of the patient? How is waiting 24 to 48 hours going to have a negative impact on his care for the patient? I suspect, the surgeon only want to look good to the patient by giving them the report quickly. The bottom line is that your Chief Pathologist is going to have to put his foot down and say NO. It is better for the patient to have good histology and a confident diagnosis than speed and a suspect diagnosis. Personally, we fix all breast tissues, even cores, overnight before processing and get great results. You can tell your surgeon that my facility will not jeopardize patient care for speed. Charles Embrey Jr., PA(ASCP) Histology Manager Carle Clinic, Illinois I would provide him with the FDA requirements. Surely he would not want to compromise the treatment of his patient. Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Same Day Breast Biopsy Results Histonetters, I need information on providing same day results on breast biopsy specimens. Is anyone out there doing this??? We have a surgeon on staff at our institution that is demanding same day rush breast biopsy results. We often rush other types of biopsies and provide same day results, but for obvious reasons (fatty, Her2 fixation, etc.) we do not provide this service for breast tissue. I do provide first am results. He has insisted that other institutions are providing same day breast results and is not happy with waiting till 8 am the next morning. I called other labs in our area and they are not doing same day breast results for the same reasons we are not. Can anyone help me with this? Your help is greatly appreciated! Thanks, Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail Beta. From Alice.Fallak <@t> uhsi.org Wed Jun 21 12:41:40 2006 From: Alice.Fallak <@t> uhsi.org (Fallak, Alice) Date: Wed Jun 21 12:39:28 2006 Subject: [Histonet] AMMONIA WATERVS. GLYCERIN Message-ID: A tech of mine mentioned that using glycerin was a better alternative to ammonia water when soaking for short periods of time . I was hoping for some feedback from you all. thanks From jxccny <@t> yahoo.com Wed Jun 21 12:54:16 2006 From: jxccny <@t> yahoo.com (x j) Date: Wed Jun 21 12:54:20 2006 Subject: [Histonet] concellation of from histonet Message-ID: <20060621175416.9176.qmail@web51410.mail.yahoo.com> Hi, dear Sir/Madam, I would like to withdraw from the histonet. Thanks, Jun --------------------------------- Sneak preview the all-new Yahoo.com. It's not radically different. Just radically better. From Lynn.Scott <@t> ahss.org Wed Jun 21 13:54:35 2006 From: Lynn.Scott <@t> ahss.org (Scott, Lynn M.) Date: Wed Jun 21 13:54:59 2006 Subject: [Histonet] Breast biopsies Message-ID: You might want to include your institutions risk management office in the conversation. The attorneys may be able to help convince the physician the importance of providing quality "Standard of Care". Properly preserving and processing tissue samples ensures quality results now and in the future, as well as, decrease potential lawsuits. Lynn M. Scott Regional Supervisor Pathology Adventist Lab Partners Hinsdale Hospital 630-856-7859 Lynn.Scott@AHSS.org ------------------------------- The information contained in this message may be privileged and / or confidential and protected from disclosure. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. From bakerj <@t> umich.edu Wed Jun 21 12:38:15 2006 From: bakerj <@t> umich.edu (John Baker) Date: Wed Jun 21 13:57:02 2006 Subject: [Histonet] (no subject) Message-ID: John A. Baker The University of Michigan Orthopaedic Research Laboratories Histology Unit 2218 BSRB, 109 Zina Pitcher Place Ann Arbor, MI 48109-2200 743-936-1635 From EWURDAK <@t> CSBSJU.EDU Wed Jun 21 14:21:12 2006 From: EWURDAK <@t> CSBSJU.EDU (Wurdak, Elizabeth) Date: Wed Jun 21 14:21:16 2006 Subject: [Histonet] mordanting in Bouin's, storage in 70%, processing mouse tissues Message-ID: <28D62335F7D5D44C8C47692CF001DF5E041166E6@EMPMAIL.ad.csbsju.edu> Hello, I learned of this listserve group at the recent meeting of the Biological Stain Commission. I wanted to join immediately because I teach histology to undergraduate students. We work through the whole paraffin technique in lab using manual methods. We are always coming up with some questions. Here are a few: 1. An attendee at the Stain Commission meeting mentioned using Bouin's fixative as a mordant prior to Mallory staining of formalin fixed tissues. We have been using saturated mercuric chloride in 5% acetic acid. Is Bouin's better? Is it less toxic? For how long should the sections be immersed in it? Do you post-treat with lithium carbonate to remove the yellow picric acid before proceeding to the next step? 2. How long can you store formalin fixed tissues in 70% alcohol? Is it best to store them at room temperature or the refrigerator? 3. Our mouse tissue blocks are coming out brittle, dry and hard to section with the exception of lung and testis samples. I attributed this to incomplete paraffin infiltration, but lengthening the parafffin baths has not helped. It was suggested to me that perhaps we are dehydrating too long. We have been using 3 changes of a 100% alcohol for 1 hour each. What would you recommend? Many thanks in advance for your suggestions. Elizabeth From rjbuesa <@t> yahoo.com Wed Jun 21 14:31:05 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 21 14:31:09 2006 Subject: [Histonet] AMMONIA WATERVS. GLYCERIN In-Reply-To: Message-ID: <20060621193106.84731.qmail@web61217.mail.yahoo.com> Alice: That is one of many soaking methods. Remember that histology is an art and also that, like cooking, each cook has preferences. In this aspect whatever works for you will be the best for you. Try it to see how you feel about it. Hope this will help you. Ren? J. "Fallak, Alice" wrote: A tech of mine mentioned that using glycerin was a better alternative to ammonia water when soaking for short periods of time . I was hoping for some feedback from you all. thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Groups gets better. Check out the new email design. Plus there?s much more to come. From TJasper <@t> smdc.org Wed Jun 21 14:47:53 2006 From: TJasper <@t> smdc.org (Jasper, Thomas G.) Date: Wed Jun 21 14:47:43 2006 Subject: [Histonet] Same Day Breast Biopsy Results Message-ID: <1A9F2A6C5762524799A816F1F09744CF1E0AF4@SCREECH.ntcampus.smdc.org> Cindy, I concur with all the responses you've received, of note is the litigation risk. It is unfortunate that potential lawsuits and the financial jolt that goes along with them must be leveraged for an obvious lack of common sense. I would ask your surgeon "friend" what Hippocrates position might be? It seems he has a hard time understanding what's in the patient's best interest. It's a shame that this guy's arrogance is causing so much trouble. Not knowing this surgeon personally I only base my comments on the fact that you've had multiple run-ins with him. The sooner he learns that you work together as a team, the better for all parties involved, most importantly the patient. Good luck! Thomas Jasper Anatomic Pathology Supervisor SMDC Clinical Laboratory Duluth, MN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of chiggerson@memhosp.com Sent: Wednesday, June 21, 2006 8:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Same Day Breast Biopsy Results Histonetters, I need information on providing same day results on breast biopsy specimens. Is anyone out there doing this??? We have a surgeon on staff at our institution that is demanding same day rush breast biopsy results. We often rush other types of biopsies and provide same day results, but for obvious reasons (fatty, Her2 fixation, etc.) we do not provide this service for breast tissue. I do provide first am results. He has insisted that other institutions are providing same day breast results and is not happy with waiting till 8 am the next morning. I called other labs in our area and they are not doing same day breast results for the same reasons we are not. Can anyone help me with this? Your help is greatly appreciated! Thanks, Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From Jackie.O'Connor <@t> abbott.com Wed Jun 21 14:55:41 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed Jun 21 14:56:18 2006 Subject: [Histonet] Same Day Breast Biopsy Results In-Reply-To: <1A9F2A6C5762524799A816F1F09744CF1E0AF4@SCREECH.ntcampus.smdc.org> Message-ID: And what's the difference between God and a surgeon? God knows he's not a surgeon. "Jasper, Thomas G." Sent by: histonet-bounces@lists.utsouthwestern.edu 06/21/2006 02:47 PM To cc histonet@lists.utsouthwestern.edu Subject RE: [Histonet] Same Day Breast Biopsy Results Cindy, I concur with all the responses you've received, of note is the litigation risk. It is unfortunate that potential lawsuits and the financial jolt that goes along with them must be leveraged for an obvious lack of common sense. I would ask your surgeon "friend" what Hippocrates position might be? It seems he has a hard time understanding what's in the patient's best interest. It's a shame that this guy's arrogance is causing so much trouble. Not knowing this surgeon personally I only base my comments on the fact that you've had multiple run-ins with him. The sooner he learns that you work together as a team, the better for all parties involved, most importantly the patient. Good luck! Thomas Jasper Anatomic Pathology Supervisor SMDC Clinical Laboratory Duluth, MN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of chiggerson@memhosp.com Sent: Wednesday, June 21, 2006 8:07 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Same Day Breast Biopsy Results Histonetters, I need information on providing same day results on breast biopsy specimens. Is anyone out there doing this??? We have a surgeon on staff at our institution that is demanding same day rush breast biopsy results. We often rush other types of biopsies and provide same day results, but for obvious reasons (fatty, Her2 fixation, etc.) we do not provide this service for breast tissue. I do provide first am results. He has insisted that other institutions are providing same day breast results and is not happy with waiting till 8 am the next morning. I called other labs in our area and they are not doing same day breast results for the same reasons we are not. Can anyone help me with this? Your help is greatly appreciated! Thanks, Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From SHARON.OSBORN <@t> SPCORP.COM Wed Jun 21 16:01:59 2006 From: SHARON.OSBORN <@t> SPCORP.COM (Osborn, Sharon) Date: Wed Jun 21 16:02:05 2006 Subject: [Histonet] High profile ribbons Message-ID: <9A919A5D70313A4D9C56A025710874080C7305@kenmsg40.us.schp.com> Heather, I forgot to ask; just assumed. Since you were using low profile blades with your Leica microtome (or any other microtome) you need to change out the back plate on the knife holder. There is a separate plate for the low profile and high profile blades. If you do not have one, contact your microtome rep. for the microtome should have come with one of each (low and high) for your knife holder. If you have not changed out the back plate, your high profile blades stick up and you are losing some of the integrity due to the wobbliness of the blade. With the proper back, the high profile blade is stabilized with the proper amount of blade protruding to do the cutting. So, perhaps this is your problem? sharon osborn, HT(ASCP)CT DNAX, SP BioPharma Palo Alto, CA Date: Tue, 20 Jun 2006 13:15:08 -0500 From: Heather.A.Harper@pcola.med.navy.mil Subject: [Histonet] High Profile Micrtome Blades To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain I want to thank everybody who sent me their samples and left over high profile blades. Now I have another dilemma. I'm having so much fun trying out new blades. I want to know if anybody can help me trouble shoot this problem in cutting. When I tried the Duraedge and Sturkey blades, I noticed, one section would be thick, the next was thin. With the AccuEdge, it cut nice except the ribbons were wrinkled and I had to stretched them out on the water bath. It's almost as though the blade is too thick or I need something adjusted on the knife holder. I cut at 5 degree angle and always have. Anybody have any ideas on what to do here. I am not getting those nice smooth wrinkle free ribbons. I'm concluding it's the blade holder needs adjusting or maybe the angle. Any input would be great. Thanks again everybody. P.S. This is a fairly new microtome. It's less than a year old. Heather A. Harper Histology Supervisor Naval Hospital Pensacola, FL ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From PMonfils <@t> Lifespan.org Wed Jun 21 16:16:07 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Jun 21 16:16:13 2006 Subject: [Histonet] mordanting in Bouin's, storage in 70%, processing mouse tissues Message-ID: <09C945920A6B654199F7A58A1D7D1FDE01717741@lsexch.lsmaster.lifespan.org> Hello Elizabeth, I'll do my best, based on my own experience. Others may have different insights ... 1. I don't know if Bouin's works "better", as I have never tried the mercuric chloride mordanting. But Bouin's works very well. Bouin's is not non-toxic by any means, but yes, it is substantially less toxic than the mercuric solution. Sections can be immersed overnight at room temperature, or an hour at 60 degrees. I use saturated lithium carbonate in 70% ethanol to remove the yellow color. 10 minutes is usually sufficient. 2. I'm sure there will be some difference of opinion here, but in my experience tissues for morphological study can be stored almost indefinitely in 70% alcohol. Refrigeration is probably "best", but I have embedded and sectioned tissues that have been stored in 70% alcohol at room temperature for years, and gotten good results. As I said, this is in reference to tissues for morphological study. Some specific substances may deteriorate over time, though I haven't noticed any problems with most histochemical stains on tissue stored in this way up to a year. All of this of course is assuming that the tissue was thoroughly fixed before it went into 70% alcohol. 3. This is a common problem with mouse tissue in particular, and over-processing is the usual reason. Inadequate infiltration will certainly make tissue difficult to section, but it won't make the tissue brittle. Three changes of 100% alcohol doesn't sound extreme, but if the tissue pieces are small (not a whole liver for example), you could probably get by cutting the times in half. Some people have experimented with adding a small amount of glycerin to the absolute alcohol, and I'm sure folks will have some other tips for you. Regards, Paul M. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > Wurdak, Elizabeth > Sent: Wednesday, June 21, 2006 12:21 PM > To: Histonet (E-mail) > Subject: [Histonet] mordanting in Bouin's, storage in 70%, processing > mouse tissues > > Hello, > I learned of this listserve group at the recent meeting of the Biological > Stain Commission. I wanted to join immediately because I teach histology > to undergraduate students. We work through the whole paraffin technique in > lab using manual methods. We are always coming up with some questions. > Here are a few: > > 1. An attendee at the Stain Commission meeting mentioned using Bouin's > fixative as a mordant prior to Mallory staining of formalin fixed > tissues. We have been using saturated mercuric chloride in 5% acetic > acid. Is Bouin's better? Is it less toxic? For how long should the > sections be immersed in it? Do you post-treat with lithium carbonate to > remove the yellow picric acid before proceeding to the next step? > > 2. How long can you store formalin fixed tissues in 70% alcohol? Is it > best to store them at room temperature or the refrigerator? > > 3. Our mouse tissue blocks are coming out brittle, dry and hard to > section with the exception of lung and testis samples. I attributed this > to incomplete paraffin infiltration, but lengthening the parafffin baths > has not helped. It was suggested to me that perhaps we are dehydrating > too long. We have been using 3 changes of a 100% alcohol for 1 hour each. > What would you recommend? > > Many thanks in advance for your suggestions. > Elizabeth > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From TJJ <@t> Stowers-Institute.org Wed Jun 21 16:18:35 2006 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Wed Jun 21 16:18:52 2006 Subject: [Histonet] Re: High profile ribbons Message-ID: Sharon, good catch! I didn't even think about that. Depending on the model you have Heather, you might just need to take off a small adapter that fits into a slot on the back of the blade holder--it's held in with two small screws. That's how it is set up on our Microm microtomes. It will definitely cause problems if you're using a high profile blade with the low profile set up! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From lilbullrider00 <@t> yahoo.com Wed Jun 21 16:25:00 2006 From: lilbullrider00 <@t> yahoo.com (brent hart) Date: Wed Jun 21 16:25:08 2006 Subject: [Histonet] E.M. precipitate Message-ID: <20060621212501.56436.qmail@web53410.mail.yahoo.com> We are having problems with black precipitate on our E.M. photo's. Does any one mind sharing your procedure with me. It has been checked and it is not bacteria. We think it is lead? Fax (817) 250-5698 Brent Hart --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. From gcallis <@t> montana.edu Wed Jun 21 16:41:35 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Jun 21 16:41:48 2006 Subject: [Histonet] mordanting in Bouin's, storage in 70%, processing mouse tissues In-Reply-To: <09C945920A6B654199F7A58A1D7D1FDE01717741@lsexch.lsmaster.l ifespan.org> References: <09C945920A6B654199F7A58A1D7D1FDE01717741@lsexch.lsmaster.lifespan.org> Message-ID: <6.0.0.22.1.20060621152244.01b2a988@gemini.msu.montana.edu> Mallory's triple stain (I really had to dig into my histopast from 1962!) The reason mercuric chloride is used as a mordant for this stain is that Zenkers (which contains mercuric chloride) was always a fixative of choice for this special stain. One could actually fix in NBF, and mordant in Zenkers before staining , when we did this stain in the first histotechnics class. I do not recall ever using Bouins as a mordant for the Triple, but John Kiernan may set us straight on this whole subject. We only used Bouins either as a fixative or mordant for Massons Trichrome, but if it works for Mallory's, I would do it instead of toxic mercuric chloride. Overprocessing of your mouse tissue sounds like the culprit for your dry, brittle tissues. Mouse tissues are generally very small and can be processed 30 to 45 min per change. Liver and brain must be totally fixed before processing due to their homogenous nature or processing finished the fixation and generally dries out the tissues even more. 70%, 80%, 95% x 2, 100% x 2, xylene X 2 with 3 changes of paraffin at 30 min each as heat will dry the tissues out even more. After trimming a block, set it on cold ice water block, but return it to the microtome and DO NOT TRIM OFF WHAT YOU HAVE JUST SOAKED!!!! You want the first sections that come off the sharp blade. If your room is really cold, you can even use a RT water soak, or try a gauze soaked in water over the just trimmed block. We simply wash Bouins fixed or mordanted sections in running tap water until the color is gone, approx 5 min or so. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From sacker <@t> hot.rr.com Wed Jun 21 16:45:14 2006 From: sacker <@t> hot.rr.com (Skye Acker) Date: Wed Jun 21 16:45:17 2006 Subject: [Histonet] (no subject) Message-ID: <000c01c6957b$fad2e8c0$6021f218@skye> Hi--I don't think my first message managed to get through, so I'm trying again LOL I'd like to post a question I hope someone has an answer for... I'm having a continual (yet recent) problem with the sectioning of biopsies for H&E stain... The first level of the biopsy appears a little hazy and the nuclear details are not as crisp as they should be...the second and third levels are still fine though. At first I thought it may be a problem with our Wash stations on the auto stainer not filling properly, since all 3 levels are on one slide with the first level closest to the top of the slide...so we started to put the different levels on separate slides. All were stained at the same time using the same method, yet again, the first level only has this problem of being slightly hazy. Does anyone have any ideas for this?? I'm clueless...the only thing I've been able to come up with is that perhaps after being faced, the block is being left on the ice too long and is being rehydrated by the melted ice....but why wouldn't that affect the other levels, since it's put back on the ice in between levels?? Argh! Skye Acker, MLT (ASCP) Metroplex Hospital Killeen, TX From EWURDAK <@t> CSBSJU.EDU Wed Jun 21 16:54:22 2006 From: EWURDAK <@t> CSBSJU.EDU (Wurdak, Elizabeth) Date: Wed Jun 21 16:54:29 2006 Subject: [Histonet] mordanting in Bouin's, storage in 70%, processingmouse tissues Message-ID: <28D62335F7D5D44C8C47692CF001DF5E041166E7@EMPMAIL.ad.csbsju.edu> Nancy, Thanks for the suggestions. Is there any particular reason for the low melting point paraffin? Elizabeth > ---------- > From: Nancy Lemke > Sent: Wednesday, June 21, 2006 2:35 PM > To: Wurdak, Elizabeth > Subject: Re: [Histonet] mordanting in Bouin's, storage in 70%, processingmouse tissues > > Question 3: > Depending on the thickness of your mouse sections, even if you are attempting whole organs, you are over processing everything. If you cut your organs into blocks of tissue no thicker than 2-3mm you can cut your time in half or less. Starting with 50% ethanol, 70%, 2x95%, 3x100%, 2xXylene and 4 x paraffin (low melting point of 54-56C, set paraffin baths to 57C), you can have all alcohols at 25 minutes, each xylene at 30 minutes and each paraffin at 20 minutes. The tissues will still be dry and brittle but no where near as dry as they must be with your protocol. Do not use heat on any solution, only on paraffin baths, but use pressure/vacuum on all stations if your processor allows. Try one mouse this way and then compare cutting to your old processing schedule. All of this assumes that your tissues are well fixed, either perfused followed by immersion for a total of no more than 24 hours for IHC or if immersed only,24-36 hours. > Nancy Lemke > Research Coordinator > Hermelin Brain Tumor Center > Henry Ford Hospital > -----Original message----- > From: "Wurdak, Elizabeth" EWURDAK@CSBSJU.EDU > Date: Wed, 21 Jun 2006 15:23:11 -0400 > To: "Histonet (E-mail)" histonet@lists.utsouthwestern.edu > Subject: [Histonet] mordanting in Bouin's, storage in 70%, processingmouse tissues > > > Hello, > > I learned of this listserve group at the recent meeting of the > Biological Stain Commission. I wanted to join immediately because I > teach histology to undergraduate students. We work through the whole > paraffin technique in lab using manual methods. We are always coming up > with some questions. Here are a few: > > > > 1. An attendee at the Stain Commission meeting mentioned using Bouin's > fixative as a mordant prior to Mallory staining of formalin fixed > tissues. We have been using saturated mercuric chloride in 5% acetic > acid. Is Bouin's better? Is it less toxic? For how long should the > sections be immersed in it? Do you post-treat with lithium carbonate to > remove the yellow picric acid before proceeding to the next step? > > > > 2. How long can you store formalin fixed tissues in 70% alcohol? Is it > best to store them at room temperature or the refrigerator? > > > > 3. Our mouse tissue blocks are coming out brittle, dry and hard to > section with the exception of lung and testis samples. I attributed > this to incomplete paraffin infiltration, but lengthening the parafffin > baths has not helped. It was suggested to me that perhaps we are > dehydrating too long. We have been using 3 changes of a 100% alcohol > for 1 hour each. What would you recommend? > > > > Many thanks in advance for your suggestions. > > Elizabeth > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ============================================================================== > Go to http://henryford.com > We're Henry Ford. We Can. > > HFHS CONFIDENTIALITY NOTICE: This email contains information from the sender that may be CONFIDENTIAL, LEGALLY PRIVILEGED, PROPRIETARY or otherwise protected from disclosure. This email is intended for use only by the person or entity to whom it is addressed. If you are not the intended recipient, any use, disclosure, copying, distribution, printing, or any action taken in reliance on the contents of this email, is strictly prohibited. If you received this email in error, please contact the sending party by replying in an email to the sender, delete the email from your computer system and shred any paper copies of the email you printed.> > > Note to Patients: There are a number of risks you should consider before using e-mail to communicate with us. These risks are described in our Privacy Policy at http://henryford.com. Review that policy carefully before continuing to communicate with us by e-mail. For greater Internet security, our policy describes the Henry Ford MyHealth electronic communication process - you may register at http://henryford.com. If you do not believe that our policy gives you the privacy and security protection you need, do not send e-mail or Internet communications to us. > ============================================================================== > > > From Janet.Bonner <@t> FLHOSP.ORG Wed Jun 21 17:10:15 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Wed Jun 21 17:12:35 2006 Subject: [Histonet] E.M. precipitate References: <20060621212501.56436.qmail@web53410.mail.yahoo.com> Message-ID: It could very well be formalin precipitate if the specimens had come into contact with formalin before being submitted in Gluteraldehyde solution.....or it could be lead from the staining. @:) ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of brent hart Sent: Wed 6/21/2006 5:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] E.M. precipitate We are having problems with black precipitate on our E.M. photo's. Does any one mind sharing your procedure with me. It has been checked and it is not bacteria. We think it is lead? Fax (817) 250-5698 Brent Hart --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From brucea <@t> unimelb.edu.au Wed Jun 21 19:27:33 2006 From: brucea <@t> unimelb.edu.au (Bruce Abaloz) Date: Wed Jun 21 19:27:57 2006 Subject: [Histonet] histoclear vs xylene Message-ID: >John, > >I agree with Rene that Histoclear doesn't work as well as Xylene. >We used to deparrafinize routinely in Xylene...X2 changes, 3 min. >each. >Now we use Histolene/Histoclear, x2 changes of 10 min. each - a >third wd. probably be good (?) We now have gone back to >deparrafinize in Xylene for ALL Immuno/Histochem. 'stuff' (only), as >were having problems with 'end results', until - we went back to >deparrafinizing in Xylene! > As far as the "known health hazards" - check your MSDS/Xylene; it >is not even considered Toxic....simply make sure you do all your >work under a fumehood/be sensible...I use it to purge my Tissue >Processer as - NOTHING works like Xylene. Cheers, Bruce in OZ > >In a "nutshell" I can tell you the following: > 1-xylene: universally used for tissue processing and staining but >a product that should be avoided because of known health hazards. > 2-histoclear: a "xylene substitute" sometimes used as a >replacement of xylene. Does not work equally well. It is "allegedly >safe" but really with no documentation of long term health effects >as xylene has. Maybe with time it will turn out to be as hazardous >as xylene. > Our profession has some health hazards and we just have to work as >safely as possible. > Hope this will help you! > > >John Campbell >Graduate Student >University of Missouri Rolla >Lab phone 573-341-4069 >Cell phone 573-694-0596 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Do you Yahoo!? > Next-gen email? Have it all with the all-new Yahoo! Mail Beta. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- BRUCE ABALOZ PH:61383446282 HISTOLOGIST FAX:61383447909 DEPT. of ZOOLOGY EMAIL: brucea@unimelb.edu.au THE UNIVERSITY Of MELBOURNE. VICTORIA. AUSTRALIA 3010 Nobody Can Make You Feel Inferior Without YOUR Permission - Eleanor Roosevelt DANCE LIKE NO-ONE'S WATCHING Q: What's a specimen? A: An Italian astronaut. From kwuny <@t> email.cs.nsw.gov.au Wed Jun 21 19:33:42 2006 From: kwuny <@t> email.cs.nsw.gov.au (Young Kwun) Date: Wed Jun 21 19:28:31 2006 Subject: [Histonet] MLH-1 In-Reply-To: Message-ID: <20060622102826.SM01308@crgcsls814> Dear Mary, I would like to make a point about the antibody you are using which is Zymed's MLH-1 (clone 14). As you know, MLH1 antibody is one of the most important colon cancer markers. It is particularly useful in determining microsatellite instability (MSI) status and tumors arising in patients with hereditary non-polyposis colorectal cancer (HNPCC) syndrome. MLH1 is notorious for its capricious staining manner in some cases and I tried several MLH1 antibodies on the market before I decided to use BD Pharmingen's (clone G168-15) for last 5 years. Occasionally I still had some problem with the stain so I decided to try Zymed's MLH1 (clone 14) last year. The staining was sometimes stronger than our routine BD's MLH1 so I decided to use Zymed's MLH1 (clone 14) as our routine one during the year 2005. With the help of some new and more sensitive detection system recently (two step polymer instead of one step), I was able to produce good stains for MSI test. Our panel also includes MHS2, MSH6, PMS2 and MGMT. It is well known that MLH1 delete and PMS2 delete are occurring together and it is very rare for a case showing only PMS2 negative. During the last year, we had several cases of colon cancer which appeared to be PMS2 delete only. I had to repeat MLH1 stains to confirm the result in some cases. When I compared the two MLH1 clones separately, the result was so dramatic. Our old clone (BD Pharmingen G168-15) showed a clean MLH1 delete in tumour cells only, whereas clone 14 MLH1 showed a strong positive staining in the tumour cells as well. Both clones showed a strong positivity in the normal epithelial cells. Clone 14 MLH1 clearly demonstrated false positive staining. Therefore I had to stop using the clone14 MLH1 immediately. I used 1/100 dilution and if I dilute 1/300 or 1/500 the normal epithelium would not stain. I also tried different Lot number and again showed false positivity. So I had to repeat all the colon carcinoma cases during the year 2005 and found that many MLH1 (and PMS2) delete cases were reported incorrectly. In fact I would like to know that whether you've ever seen a delete case with the clone 14 MLH1. These results were also confirmed by MSI molecular biological test at St. Vincent Hospital here in Sydney. PS: We are now using BondMax autostainer for MSI test and I don't need to incubate overnight anymore. The staining is fantastic! Young Kwun Senior Hospital Scientist Dept of Anatomical Pathology Concord Hospital Concord NSW 2139 Australia Phone:61-2-9767 6075 Fax:61-2-9767 8427 Email:kwuny@email.cs.nsw.gov.au -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mary Judd Sent: Wednesday, 21 June 2006 8:31 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] MLH-1 We use MLH-1(14) from Zymed cat.no. 18-2342 We pressure cook in citrate buffer 6pH, use the antibody at 1:30 and incubate overnight at 4C. Mary Judd Section Head Immunohistochemistry Cellular Pathology Level E, Mailpoint 2 Southampton University Hospitals Trust Tel. 02380 795144 Fax. 02380 796869 D I S C L A I M E R This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. 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Please visit our website at http://www.suht.nhs.uk _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____________________________________________________________________ This email has been scanned for the Sydney South West Area Health Service by the MessageLabs Email Security System. SSWAHS regularly monitors emails and attachments to ensure compliance with the NSW Government's Electronic Messaging Policy. "This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please destroy it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of the Sydney South West Area Health Service." From bhewlett <@t> cogeco.ca Wed Jun 21 20:39:37 2006 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Wed Jun 21 20:39:42 2006 Subject: [Histonet] mordanting in Bouin's, storage in 70%, processing mouse tissues References: <09C945920A6B654199F7A58A1D7D1FDE01717741@lsexch.lsmaster.lifespan.org> <6.0.0.22.1.20060621152244.01b2a988@gemini.msu.montana.edu> Message-ID: <005001c6959c$b8fc3110$6500a8c0@mainbox> Elizabeth, Paul and Gayle, With respect to the use of "mordant's" for trichrome and triacid connective tissue stains. To quote John Kiernan: "A mordant is a substance that serves to bind a dye to a substrate. Thus cotton can be impregnated with tannic acid, with which cationic dyes form insoluble salts. The tannic acid serves as a mordant for the dye, which would not adhere to the uncharged molecules of the cotton. In histological parlance, however, the term is restricted to metal ions that are able to bind covalently to suitable dye molecules, forming complexes (AKA 'coordination compounds' or 'dye-metal complexes')." (See also; Baker, J.R. Principles of biological microtechnique: 1958) Zenker and Helly fluids (as Gayle correctly mentions) were traditionally favoured as the fixatives of choice for connective tissue stains. These fixatives contain mercuric chloride and chromium salts, both of which qualify as mordants in the strict sense of the term. As Gayle mentions, it is possible to fix tissue in formaldehyde and then apply the mordant(s) as a pretreatment prior to staining with connective tissue stains. However, it is doubtful if any true mordant action is occurring ( see Horobin: Histochemistry.1982), since these fluids also re-align the reactive side chains on the proteins and favour acidophilia. Bouin's fluid is also a favoured fixative for connective tissue stains, however, its mode of action is completely different. It is, correctly speaking, an accentuator. i.e. a substance that improves the binding of a dye to a substrate. Bouin's fluid works equally well as a staining pre-treatment on formaldehyde-fixed tissue prior to performing the connective tissue stain. Bouin's fluid as a purported 'mordant'(sic) for these stains is a complete misnomer!!! NO metal ions are present in Bouin's fluid. The principle active ingredient is picric acid. The anionic dyes WILL bind to the fixed proteins in connective tissue, how well depends on the nature of the fixative! Formaldehyde fixation leaves the proteins with a preponderance of acidic carboxyl side chains and hence slight overall basophilia. Treatment in picric acid re-aligns the reactive side chains on the proteins, so that there is now a predominance of basic amino groups and hence maximal binding of anionic dyes (acidophilia). Mercury fixation is simply not necessary! Nor is any other metal mordant. (see Troubleshooting Histology stains: Horobin and Bancroft 1998, page 129). Best regards, Bryan ----- Original Message ----- From: "Gayle Callis" To: "Monfils, Paul" ; Sent: Wednesday, June 21, 2006 5:41 PM Subject: RE: [Histonet] mordanting in Bouin's, storage in 70%,processing mouse tissues > Mallory's triple stain (I really had to dig into my histopast from 1962!) > The reason mercuric chloride is used as a mordant for this stain is that > Zenkers (which contains mercuric chloride) was always a fixative of choice > for this special stain. One could actually fix in NBF, and mordant in > Zenkers before staining , when we did this stain in the first > histotechnics class. > > I do not recall ever using Bouins as a mordant for the Triple, but John > Kiernan may set us straight on this whole subject. We only used Bouins > either as a fixative or mordant for Massons Trichrome, but if it works for > Mallory's, I would do it instead of toxic mercuric chloride. > > Overprocessing of your mouse tissue sounds like the culprit for your dry, > brittle tissues. Mouse tissues are generally very small and can be > processed 30 to 45 min per change. Liver and brain must be totally fixed > before processing due to their homogenous nature or processing finished > the fixation and generally dries out the tissues even more. > > 70%, 80%, 95% x 2, 100% x 2, xylene X 2 with 3 changes of paraffin at 30 > min each as heat will dry the tissues out even more. > > After trimming a block, set it on cold ice water block, but return it to > the microtome and DO NOT TRIM OFF WHAT YOU HAVE JUST SOAKED!!!! You want > the first sections that come off the sharp blade. If your room is really > cold, you can even use a RT water soak, or try a gauze soaked in water > over the just trimmed block. > > We simply wash Bouins fixed or mordanted sections in running tap water > until the color is gone, approx 5 min or so. > > > > > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 > 406 994-4303 (FAX) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From cfavara <@t> niaid.nih.gov Wed Jun 21 22:06:56 2006 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID) [E]) Date: Wed Jun 21 22:07:04 2006 Subject: [Histonet] histoclear vs xylene In-Reply-To: Message-ID: Here is the web site on xylenes from the EPA http://www.epa.gov/ttn/atw/hlthef/xylenes.html I would not necessarily agree that "it is not even considered Toxic" but I do agree nothing is quite like xylene or toluene for histo. c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives -----Original Message----- From: Bruce Abaloz [mailto:brucea@unimelb.edu.au] Sent: Wednesday, June 21, 2006 5:28 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] histoclear vs xylene >John, > >I agree with Rene that Histoclear doesn't work as well as Xylene. >We used to deparrafinize routinely in Xylene...X2 changes, 3 min. >each. >Now we use Histolene/Histoclear, x2 changes of 10 min. each - a >third wd. probably be good (?) We now have gone back to >deparrafinize in Xylene for ALL Immuno/Histochem. 'stuff' (only), as >were having problems with 'end results', until - we went back to >deparrafinizing in Xylene! > As far as the "known health hazards" - check your MSDS/Xylene; it >is not even considered Toxic....simply make sure you do all your >work under a fumehood/be sensible...I use it to purge my Tissue >Processer as - NOTHING works like Xylene. Cheers, Bruce in OZ > >In a "nutshell" I can tell you the following: > 1-xylene: universally used for tissue processing and staining but >a product that should be avoided because of known health hazards. > 2-histoclear: a "xylene substitute" sometimes used as a >replacement of xylene. Does not work equally well. It is "allegedly >safe" but really with no documentation of long term health effects >as xylene has. Maybe with time it will turn out to be as hazardous >as xylene. > Our profession has some health hazards and we just have to work as >safely as possible. > Hope this will help you! > > >John Campbell >Graduate Student >University of Missouri Rolla >Lab phone 573-341-4069 >Cell phone 573-694-0596 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Do you Yahoo!? > Next-gen email? Have it all with the all-new Yahoo! Mail Beta. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- BRUCE ABALOZ PH:61383446282 HISTOLOGIST FAX:61383447909 DEPT. of ZOOLOGY EMAIL: brucea@unimelb.edu.au THE UNIVERSITY Of MELBOURNE. VICTORIA. AUSTRALIA 3010 Nobody Can Make You Feel Inferior Without YOUR Permission - Eleanor Roosevelt DANCE LIKE NO-ONE'S WATCHING Q: What's a specimen? A: An Italian astronaut. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From a.babri <@t> uq.edu.au Wed Jun 21 22:29:16 2006 From: a.babri <@t> uq.edu.au (Saleem Babri) Date: Wed Jun 21 22:27:53 2006 Subject: [Histonet] Bouins, Brittling, and Blocks Message-ID: <449A0E8C.60603@uq.edu.au> Hello, I learned of this listserve group at the recent meeting of the Biological Stain Commission. I wanted to join immediately because I teach histology to undergraduate students. We work through the whole paraffin technique in lab using manual methods. We are always coming up with some questions. Here are a few: 1. An attendee at the Stain Commission meeting mentioned using Bouin's fixative as a mordant prior to Mallory staining of formalin fixed tissues. We have been using saturated mercuric chloride in 5% acetic acid. Is Bouin's better? Is it less toxic? For how long should the sections be immersed in it? Do you post-treat with lithium carbonate to remove the yellow picric acid before proceeding to the next step? 2. How long can you store formalin fixed tissues in 70% alcohol? Is it best to store them at room temperature or the refrigerator? 3. Our mouse tissue blocks are coming out brittle, dry and hard to section with the exception of lung and testis samples. I attributed this to incomplete paraffin infiltration, but lengthening the parafffin baths has not helped. It was suggested to me that perhaps we are dehydrating too long. We have been using 3 changes of a 100% alcohol for 1 hour each. What would you recommend? Many thanks in advance for your suggestions. Elizabeth Hello Elizabeth, I read your email on the histonet post. l faced similar problems with peripheral nerves of human cadavers during my study. Please have a look at the following suggestions which I found helpful thanks to the technical staff in our histology lab in the Anatomy Department at the University of Queensland Australia. 1. Bouin's fixative is a very effective mordanting solution. For human cadaveric specimens that were embalmed six months prior to commencement of the study, nerve tissue was harvested and mordanted in Bouin's at 4C and continually agitated for a minimum of 24 hours. At the completion of 24 hours the picric acid was removed using saturated solution of lithium carbonate to facilitate proper staining. 2. Literature has shown that you can store formaldehyde fixed tissue in alcohol for an indefinite period of time. Controversies do exist which indicate that the quality of the tissue is affected (becoming more brittle). It is always better to store them at 4C which can slow down the brittling of tissue preserved in alcohol. 3. Your sections might be hard because you might be using too high a concentration of formaldehyde/glutaraldehyde to fix the tissues. Moreover embedding could be a problem. Before you section the tissues might put them in the refrigerator for at least 15 minutes. Try using 80, 90, 100% alcohol for the three changes instead of 100% alcohol for all the three changes. From barbara.bublava <@t> meduniwien.ac.at Thu Jun 22 02:49:03 2006 From: barbara.bublava <@t> meduniwien.ac.at (Barbara Bublava) Date: Thu Jun 22 02:49:14 2006 Subject: [Histonet] neutrophils In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA270171FE10@hpes1.HealthPartners.int> References: <0E394B648E5284478A6CCB78E5AFDA270171FE10@hpes1.HealthPartners.int> Message-ID: <449A4B6F.5040905@meduniwien.ac.at> Hi Dorothy I did have a similar problem with some specimens lately. So I did a little test with some human spleen: one piece was properly fixed with formaldehyde, one piece of the same spleen wasn?t fixed in formaldehyde but transferred directly to 70% Ethanole, and one piece was improperly fixed in formaldehyde and then transferred to 70% Ethanole. All pieces were processed together after 24 hours. The piece transferred directly to 70% Ethanol didn?t work at all, the improperly fixed speciemen did stain irregularly - i could clearly see which parts were fixed and which not. The correct fixated piece did work superbe. I did not have time to make further tests to see if autolysis is the problem or if it is the alcohol - or maybe the water in the alcohol? best regards Barbara Bublava Medical University of Vienna Webb, Dorothy L wrote: > We are staining some research tissue (rabbitt?) for neutrophils using > the chloroacetate esterase kit from Sigma with an inflamed appendix for > a control. The control turned out beautiful, but, nothing is showing up > in the research tissue. We did not gross in the research tissue, only > processed it according to our routine processing schedule. According to > the "fellow" who is doing this project, the formalin was expired on the > research tissue. Does anyone have any ideas on this case?? I am at a > loss and the pathologist helping the "fellow" to read these tissues out > is wanting me to come up with a solution, of course!! So, HELP and > thanks, as always!! > ________________________________________ > This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. > > If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From vanann702 <@t> skmc.gov.ae Thu Jun 22 05:05:54 2006 From: vanann702 <@t> skmc.gov.ae (Anne Van Binsbergen) Date: Thu Jun 22 05:03:03 2006 Subject: [Histonet] histoclear vs xylene Message-ID: ozziebruce 'not considered as toxic'- this level of ignorance NEVER fails to amaze me.....perhaps you need to do a little more reading up on xylene and associated harmful effects - there are many!!!! annieinarabia -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bruce Abaloz Sent: Thursday, June 22, 2006 4:28 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] histoclear vs xylene >John, > >I agree with Rene that Histoclear doesn't work as well as Xylene. >We used to deparrafinize routinely in Xylene...X2 changes, 3 min. >each. >Now we use Histolene/Histoclear, x2 changes of 10 min. each - a >third wd. probably be good (?) We now have gone back to >deparrafinize in Xylene for ALL Immuno/Histochem. 'stuff' (only), as >were having problems with 'end results', until - we went back to >deparrafinizing in Xylene! > As far as the "known health hazards" - check your MSDS/Xylene; it >is not even considered Toxic....simply make sure you do all your >work under a fumehood/be sensible...I use it to purge my Tissue >Processer as - NOTHING works like Xylene. Cheers, Bruce in OZ > >In a "nutshell" I can tell you the following: > 1-xylene: universally used for tissue processing and staining but >a product that should be avoided because of known health hazards. > 2-histoclear: a "xylene substitute" sometimes used as a >replacement of xylene. Does not work equally well. It is "allegedly >safe" but really with no documentation of long term health effects >as xylene has. Maybe with time it will turn out to be as hazardous >as xylene. > Our profession has some health hazards and we just have to work as >safely as possible. > Hope this will help you! > > >John Campbell >Graduate Student >University of Missouri Rolla >Lab phone 573-341-4069 >Cell phone 573-694-0596 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Do you Yahoo!? > Next-gen email? Have it all with the all-new Yahoo! Mail Beta. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- BRUCE ABALOZ PH:61383446282 HISTOLOGIST FAX:61383447909 DEPT. of ZOOLOGY EMAIL: brucea@unimelb.edu.au THE UNIVERSITY Of MELBOURNE. VICTORIA. AUSTRALIA 3010 Nobody Can Make You Feel Inferior Without YOUR Permission - Eleanor Roosevelt DANCE LIKE NO-ONE'S WATCHING Q: What's a specimen? A: An Italian astronaut. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jxccny <@t> yahoo.com Thu Jun 22 05:44:19 2006 From: jxccny <@t> yahoo.com (x j) Date: Thu Jun 22 05:44:24 2006 Subject: [Histonet] please remove me from your email list. Thanks Message-ID: <20060622104419.35917.qmail@web51405.mail.yahoo.com> How to withdraw from the histonet? please remove me from your email list. Thanks, Jun --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. From oshel1pe <@t> cmich.edu Thu Jun 22 07:06:46 2006 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Thu Jun 22 07:06:56 2006 Subject: [Histonet] E.M. precipitate In-Reply-To: <20060621212501.56436.qmail@web53410.mail.yahoo.com> References: <20060621212501.56436.qmail@web53410.mail.yahoo.com> Message-ID: Brent, What is the size and shape of the precipitate? The usual suspects are uranium or lead crystals or blobs from the staining, or could be a phosphate deposit after osmium fixation. The first things to check are: is the lead stain at or above pH 12? If not, it will cause black precipitate; do you use a phosphate buffer during fixation? If yes, make sure it is washed out before osmium fixation, or use a sodium-only buffer (mono- and dibasice sodium, no potassium). If that does't work, search the archives of the Microscopy Society of America: http://www.msa.microscopy.org/MicroscopyListserver/SearchMLArchive.html There have been several discussions on this list about your problem. Phil >We are having problems with black precipitate on >our E.M. photo's. Does any one mind sharing your >procedure with me. It has been checked and it is >not bacteria. We think it is lead? > > Fax (817) 250-5698 > Brent Hart > > >--------------------------------- >Talk is cheap. Use Yahoo! Messenger to make >PC-to-Phone calls. Great rates starting at >1?/min. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Philip Oshel Microscopy Facility Supervisor Department of Biology Central Michigan University 024C Brooks Hall Mt. Pleasant, MI 48859 (989) 774-3576 From mcauliff <@t> umdnj.edu Thu Jun 22 07:56:55 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Jun 22 07:56:17 2006 Subject: [Histonet] mordanting in Bouin's, storage in 70%, processing mouse tissues In-Reply-To: <28D62335F7D5D44C8C47692CF001DF5E041166E6@EMPMAIL.ad.csbsju.edu> References: <28D62335F7D5D44C8C47692CF001DF5E041166E6@EMPMAIL.ad.csbsju.edu> Message-ID: <449A9397.9090407@umdnj.edu> Hi Elizabeth: Wurdak, Elizabeth wrote: >Hello, >I learned of this listserve group at the recent meeting of the Biological Stain Commission. I wanted to join immediately because I teach histology to undergraduate students. We work through the whole paraffin technique in lab using manual methods. We are always coming up with some questions. Here are a few: > >1. An attendee at the Stain Commission meeting mentioned using Bouin's fixative as a mordant prior to Mallory staining of formalin fixed tissues. We have been using saturated mercuric chloride in 5% acetic acid. Is Bouin's better? Is it less toxic? For how long should the sections be immersed in it? Do you post-treat with lithium carbonate to remove the yellow picric acid before proceeding to the next step? > > Mercuric cholride is very toxic and polutes the environment. Even the water and alcohol washes after HgCl should not go down the drain. One does not need to mordant in Bouin's fix, one half or 3/4 saturated picric acid will do nicely. Treat blocks at room temp overnight, wash in water. I think you are better off treating the blocks rather than the sections but I do not have evidence for this idea, just the idea of preserving things before hitting them with alcohol. I post-treat the sections with LiCO3, much faster than treating the whole block. I suspect that any carbonate will do for this? If your safety people don't want you to have a bottle of picric acid in the lab, just buy some sat. aqueous solution. >2. How long can you store formalin fixed tissues in 70% alcohol? Is it best to store them at room temperature or the refrigerator? > > You can store it as long as you want to. However. long storage does extract cytoplasm and will decrease staining. How long is long? I don't know. >3. Our mouse tissue blocks are coming out brittle, dry and hard to section with the exception of lung and testis samples. I attributed this to incomplete paraffin infiltration, but lengthening the parafffin baths has not helped. It was suggested to me that perhaps we are dehydrating too long. We have been using 3 changes of a 100% alcohol for 1 hour each. What would you recommend? > > Sounds like over-processing to me as well. Geoff -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From asmith <@t> mail.barry.edu Thu Jun 22 09:45:09 2006 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Thu Jun 22 09:45:15 2006 Subject: [Histonet] histoclear vs xylene In-Reply-To: Message-ID: <5D2189E74151CC42BEC02906BA8996322B9204@exchsrv01.barrynet.barry.edu> Although we carefully save our MSDS's in a neat alphabetical file, what we actually consult is Lewis and Sax's HAZARDOUS CHEMICALS DESK REFERENCE (kept in my office) or HAZARDOUS PROPERTIES OF INDUSTRIAL MATERIALS (kept in the lab). Lewis and Sax say of xylene: "Moderately toxic by inhalation, ingestion, and subcutaneous routes... Irritation can start at 200 ppm." Bruce, however is taking due precautions by using xylene in a fume hood. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne Van Binsbergen Sent: Thursday, June 22, 2006 6:06 AM To: Bruce Abaloz; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] histoclear vs xylene ozziebruce 'not considered as toxic'- this level of ignorance NEVER fails to amaze me.....perhaps you need to do a little more reading up on xylene and associated harmful effects - there are many!!!! annieinarabia -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bruce Abaloz Sent: Thursday, June 22, 2006 4:28 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] histoclear vs xylene >John, > >I agree with Rene that Histoclear doesn't work as well as Xylene. >We used to deparrafinize routinely in Xylene...X2 changes, 3 min. >each. >Now we use Histolene/Histoclear, x2 changes of 10 min. each - a >third wd. probably be good (?) We now have gone back to >deparrafinize in Xylene for ALL Immuno/Histochem. 'stuff' (only), as >were having problems with 'end results', until - we went back to >deparrafinizing in Xylene! > As far as the "known health hazards" - check your MSDS/Xylene; it >is not even considered Toxic....simply make sure you do all your >work under a fumehood/be sensible...I use it to purge my Tissue >Processer as - NOTHING works like Xylene. Cheers, Bruce in OZ > >In a "nutshell" I can tell you the following: > 1-xylene: universally used for tissue processing and staining but >a product that should be avoided because of known health hazards. > 2-histoclear: a "xylene substitute" sometimes used as a >replacement of xylene. Does not work equally well. It is "allegedly >safe" but really with no documentation of long term health effects >as xylene has. Maybe with time it will turn out to be as hazardous >as xylene. > Our profession has some health hazards and we just have to work as >safely as possible. > Hope this will help you! > > >John Campbell >Graduate Student >University of Missouri Rolla >Lab phone 573-341-4069 >Cell phone 573-694-0596 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Do you Yahoo!? > Next-gen email? Have it all with the all-new Yahoo! Mail Beta. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- BRUCE ABALOZ PH:61383446282 HISTOLOGIST FAX:61383447909 DEPT. of ZOOLOGY EMAIL: brucea@unimelb.edu.au THE UNIVERSITY Of MELBOURNE. VICTORIA. AUSTRALIA 3010 Nobody Can Make You Feel Inferior Without YOUR Permission - Eleanor Roosevelt DANCE LIKE NO-ONE'S WATCHING Q: What's a specimen? A: An Italian astronaut. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From Tiffany.L.Sheffield <@t> uth.tmc.edu Thu Jun 22 09:48:24 2006 From: Tiffany.L.Sheffield <@t> uth.tmc.edu (Sheffield, Tiffany L) Date: Thu Jun 22 09:48:28 2006 Subject: [Histonet] Texas Histo Opportunity Message-ID: Hi Fellow Histonetters! Since you all are the best of the best, a histology position has become available at The University of Texas Health Science Center in Houston Medical School Department of Orthopaedic Surgery. We are looking for someone with their ASCP certification. It would be a bonus if you had previous experience with calcified tissue. Great pay and benefits, the works! It is a wonderful place to work (great people). Please contact me if you are interested. I would be happy to answer any questions you might have. ************************************************** Tiffany Sheffield-Lopez,BS,HT(ASCP) Supervisor, Bone Histomorphometry & Biomaterials Lab Department of Orthopaedic Surgery The University of Texas Houston Health Science Center 6431 Fannin, Suite 6.144 MSB Houston, TX 77030 713-500-6803 Wk 713-500-0729 Fax From lblazek <@t> digestivespecialists.com Thu Jun 22 10:22:36 2006 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Thu Jun 22 10:19:20 2006 Subject: [Histonet] job opening Message-ID: <6CBA6DC98A079D408C87250591D9DFB80233E0A7@bruexchange.digestivespecialists.com> Job opening in the Dayton, Ohio area. Part time, possibly to go to full time HT (ASCP) certified histo tech for a new busy GI pathology lab. If interested contact me directly at lblazek@digestivespecialists.com Linda Blazek HT (ASCP) Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com From DOOLEEO <@t> shands.ufl.edu Thu Jun 22 10:42:25 2006 From: DOOLEEO <@t> shands.ufl.edu (Elaine Dooley) Date: Thu Jun 22 10:42:55 2006 Subject: [Histonet] C-myc and Cyclin D1 Probes automated Message-ID: Dear Histonetter and Vendors, Our there any automated stainers out there that can use c-myc and cyclin D1 probes in a fully automated or partially automated system? Elaine Dooley HTL Shands Hospital Gainesville FL 352-265-0111 ext 72117 From stamptrain <@t> yahoo.com Thu Jun 22 11:13:07 2006 From: stamptrain <@t> yahoo.com (Roger Moretz) Date: Thu Jun 22 11:13:12 2006 Subject: [Histonet] please remove me from your email list. Thanks In-Reply-To: <20060622104419.35917.qmail@web51405.mail.yahoo.com> Message-ID: <20060622161307.54617.qmail@web50315.mail.yahoo.com> Well, this is 3 emails from x j asking the same questions. I have politely sent instructions off-line on how to do this. Anyone else care to try? Roger Moretz, Ph.D. Dept of Toxicology BI Pharmaceuticals --- x j wrote: > How to withdraw from the histonet? please remove me > from your email list. Thanks, Jun > > --------------------------------- > Talk is cheap. Use Yahoo! Messenger to make > PC-to-Phone calls. Great rates starting at 1?/min. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From barbara.bublava <@t> meduniwien.ac.at Thu Jun 22 11:20:22 2006 From: barbara.bublava <@t> meduniwien.ac.at (Barbara Bublava) Date: Thu Jun 22 11:20:28 2006 Subject: [Histonet] neutrophils In-Reply-To: References: Message-ID: <449AC346.4050105@meduniwien.ac.at> Dear Robert, Dear Gayle trying to keep my Message short, maybee I ended up beeing too short I did not want to test if tissue can be fixed in 70% Ethanol. I did want to show what happens if tissue isn?t fixed enough (Dorothy mentioned expired formalin) and wents to the processor - often starting with 70% alcohol - or transferred, as Robert mentioned, to it for longterm storage. I tested the "extremes"! 1.) no fixation (70% alcohol, trying to simulate starting the processor with unfixed specimen) 2.) "half" or partial fixation (transferred to 70% before fixed completely - which means a rather big specimen for 4 hours, fixed at the edges, not in the center) - trying to simulate staring the processor with partial fixed specimen. The case one doctor "produced" by insisting on fast processing 3.) and "proper" fixation. (24 hrs - for a middle sized specimen, fixed in the center also) - correct procedure I did this short test to demonstrate how the incosistant staining the doctor claimed did happen and to show how important fixation is for a correct staining (nothing really new to us, but some doctors...) I did choose Chloracetate Esterase Kit because it was the stain burdend, HE also did show differences but after all it could be interpreted. CAE did fail comletely! I don?t know if this is the solution for Dorothy?s problem, since I did my little test on human tissue and do not know if the stain will work on rodent, like other people considered. If alcohol and not autolysis is the problem - then it could maybe help to move the specimens to fresh formalin before processing? I know there are impairments in my little demonstration - for example the time specimens were left in 70% Ethanol (before moving to the processor) differs from 0 to 24 hrs (I wanted to process, cut and stain them together - so there can be no differences) a lot more testing could be done, and I would do it if I had the time for it... but at least and for my intention the test did what it should do. The doctor understood the overhelming importance of correct fixation. Barbara Bublava Medical University of Vienna From barbara.bublava <@t> meduniwien.ac.at Thu Jun 22 11:30:09 2006 From: barbara.bublava <@t> meduniwien.ac.at (Barbara Bublava) Date: Thu Jun 22 11:30:16 2006 Subject: [Histonet] neutrophils In-Reply-To: <449AC346.4050105@meduniwien.ac.at> References: <449AC346.4050105@meduniwien.ac.at> Message-ID: <449AC591.1050101@meduniwien.ac.at> another point coming to my mind - how does Chloracetate esterase react when formalin gets acidic like expired folmalin could do? Barbara Bublava wrote: > Dear Robert, Dear Gayle > > trying to keep my Message short, maybee I ended up beeing too short > > I did not want to test if tissue can be fixed in 70% Ethanol. I did want > to show what happens if tissue isn?t fixed enough (Dorothy mentioned > expired formalin) and wents to the processor - often starting with 70% > alcohol - or transferred, as Robert mentioned, to it for longterm storage. > > I tested the "extremes"! > > 1.) no fixation (70% alcohol, trying to simulate starting the processor > with unfixed specimen) > > 2.) "half" or partial fixation (transferred to 70% before fixed > completely - which means a rather big specimen for 4 hours, fixed at the > edges, not in the center) - trying to simulate staring the processor > with partial fixed specimen. The case one doctor "produced" by insisting > on fast processing > > 3.) and "proper" fixation. (24 hrs - for a middle sized specimen, fixed > in the center also) - correct procedure > > I did this short test to demonstrate how the incosistant staining the > doctor claimed did happen and to show how important fixation is for a > correct staining (nothing really new to us, but some doctors...) > > I did choose Chloracetate Esterase Kit because it was the stain burdend, > HE also did show differences but after all it could be interpreted. > CAE did fail comletely! > > I don?t know if this is the solution for Dorothy?s problem, since I did > my little test on human tissue and do not know if the stain will work on > rodent, like other people considered. > > If alcohol and not autolysis is the problem - then it could maybe help > to move the specimens to fresh formalin before processing? > > I know there are impairments in my little demonstration - for example > the time specimens were left in 70% Ethanol (before moving to the > processor) differs from 0 to 24 hrs (I wanted to process, cut and stain > them together - so there can be no differences) > > a lot more testing could be done, and I would do it if I had the time > for it... > > but at least and for my intention the test did what it should do. The > doctor understood the overhelming importance of correct fixation. > > Barbara Bublava > Medical University of Vienna > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From MElliott <@t> mrl.ubc.ca Thu Jun 22 12:14:52 2006 From: MElliott <@t> mrl.ubc.ca (Mark Elliott) Date: Thu Jun 22 12:16:25 2006 Subject: [Histonet] Activated Dendritic cells Message-ID: <449A6D9C020000D60001D67D@mail.mrl.ubc.ca> I have been asked if there is a antibody available which would label specifically activated dendritic cells. Anyone any ideas?? Thanks MArk From victoria.spoon <@t> bassett.org Thu Jun 22 12:47:08 2006 From: victoria.spoon <@t> bassett.org (Spoon, Victoria) Date: Thu Jun 22 12:47:22 2006 Subject: [Histonet] Per diem histotechnician position Message-ID: <052739589974CC44A7770DA22157C804A5981E@ex3.bassett.org> Bassett Hospital in Cooperstown, New York has a per diem histotechnician position open. The position would be 8 hour days as needed when other staff members are scheduled off through out the year. The total number of days would equal to half of the year. Duties include full variety of histology responsibilities in a hospital based laboratory. For more information call Human Resources at (607)547-3120 or visit www.bassett.org. NOTICE OF CONFIDENTIALITY This electronic message, including attachments, is for the sole use of the named recipient and may contain confidential or privileged information protected by New York State, and Federal regulations. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. If you are not the intended recipient or have received this communication in error please contact the sender or email.security@bassett.org and destroy all copies of the original message. Thank you. From LRaff <@t> lab.uropartners.com Thu Jun 22 13:41:56 2006 From: LRaff <@t> lab.uropartners.com (Lester Raff) Date: Thu Jun 22 13:42:00 2006 Subject: [Histonet] Pressure Cooker for IHC Message-ID: <5DA1CA5D0B98A84985B545A24423B822019850@UPLAB01.uplab.local> Hi all: We are using the PASCAL pressure cooker from DAKO for HIER. We have noticed on occasion steam coming out of the edges (not through the pressure release valve). Our rep says this is normal. Do others have the same experience? Also, we are still looking for a part time histotech. Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 From Karen.Heckford <@t> CHW.edu Thu Jun 22 13:46:03 2006 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Thu Jun 22 13:46:24 2006 Subject: [Histonet] QIHC Exam Message-ID: Howdy Histonetters, I was wondering if anyone out there just took the QIHC exam. Was it a timed exam? What was the best study guides? I would really appreciate any input. Cheers, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathololgy Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu From jtcrhb <@t> umr.edu Thu Jun 22 13:49:26 2006 From: jtcrhb <@t> umr.edu (Campbell, John Thomas (UMR-Student)) Date: Thu Jun 22 13:58:24 2006 Subject: [Histonet] slicing paraffin embeded specimens Message-ID: <8D80EE169A64FA448EBEDC5812BA7F9B1E8D10@UMR-CMAIL1.umr.edu> I am just getting started in histology and I am having trouble keeping my slices from bunching up on the microtome. What can I do to keep this from happening? I am using a manual slicer, slicing the specimens into 14 microns thick slices. John Campbell Graduate Student University of Missouri Rolla Lab phone 573-341-4069 Cell phone 573-694-0596 From EWURDAK <@t> CSBSJU.EDU Thu Jun 22 14:25:40 2006 From: EWURDAK <@t> CSBSJU.EDU (Wurdak, Elizabeth) Date: Thu Jun 22 14:25:45 2006 Subject: [Histonet] slicing paraffin embeded specimens Message-ID: <28D62335F7D5D44C8C47692CF001DF5E041166E8@EMPMAIL.ad.csbsju.edu> I am assuming that your block is properly trimmed and aligned to begin with. Try these one at a time. 1. Use a paintbrush to lift the sections from the knife edge, being careful not to touch the cutting edge with the brush. 2. Increase humidity in the room to reduce static. 3. Adjust knife angle. 4. Clean both the front and the back of the knife with a Q-tip dipped in xylene. Allow the xylene to evaporate completely before you resume sectioning. 5. Retrim the edges of your block. 6. Try another block. 7. If none of these help, put off sectioning until another day. Elizabeth Elizabeth Wurdak, PhD Biology Department Saint John's University Collegeville, MN 56321 Tel: (320) 363-3177 > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Campbell, John Thomas (UMR-Student) > Sent: Thursday, June 22, 2006 1:49 PM > To: histonet > Subject: [Histonet] slicing paraffin embeded specimens > > I am just getting started in histology and I am having trouble keeping my slices from bunching up on the microtome. What can I do to keep this from happening? I am using a manual slicer, slicing the specimens into 14 microns thick slices. > > John Campbell > Graduate Student > University of Missouri Rolla > Lab phone 573-341-4069 > Cell phone 573-694-0596 > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From RohrT <@t> nyackhospital.org Thu Jun 22 14:30:09 2006 From: RohrT <@t> nyackhospital.org (Theresa Rohr) Date: Thu Jun 22 14:33:09 2006 Subject: [Histonet] Multitissue blocks Message-ID: Hi out there, I was wondering if any of you order multi-tissue blocks for IHC and if so where from? Thanks for the information. Theresa Rohr, Nyack Hospital NY rohrt@nyackhospital.org Theresa Rohr, BA, HT(ASCP) Section Head, Histology Nyack Hospital 160 North Midland Avenue Nyack, New York 10960 phone 845-348-2276 fax 845-348-8430 Nyack Hospital is required by Law to protect the privacy of health information under HIPAA Rules and Regulations Part 11, Article 45 CFR, Parts 160-164. This email, including attachments, is intended for the exclusive use of the person or the entity to which it is addressed. It may contain confidential or legally protected information. The authorized recipient is prohibited from disclosing this information to any other party and is required to destroy this information after its stated need has been fulfilled. If the recipient or reader of this email is not the intended recipient or her/his authorized agent, the reader is hereby notified that any dissemination, distribution or copying of this email is prohibited. If you believe that you have received this email in error, please advise the sender immediately by reply email and then delete this email immediately. From EWURDAK <@t> CSBSJU.EDU Thu Jun 22 14:52:08 2006 From: EWURDAK <@t> CSBSJU.EDU (Wurdak, Elizabeth) Date: Thu Jun 22 14:52:14 2006 Subject: [Histonet] E.M. precipitate Message-ID: <28D62335F7D5D44C8C47692CF001DF5E041166E9@EMPMAIL.ad.csbsju.edu> Lead is often the culprit in forming precipitates. Several different lead stains are in use. Reynolds lead citrate is the one I prefer. I make it up fresh weekly. I also place several pellets of sodium hydroxide in the staining dish to absorb atmospheric CO2 which can react with the stain to form insoluble lead carbonate. Elizabeth Elizabeth Wurdak, PhD Biology Department Saint John's University Collegeville, MN 56321 Tel: (320) 363-3177 > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of brent hart > Sent: Wednesday, June 21, 2006 4:25 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] E.M. precipitate > > We are having problems with black precipitate on our E.M. photo's. Does any one mind sharing your procedure with me. It has been checked and it is not bacteria. We think it is lead? > > Fax (817) 250-5698 > Brent Hart > > > --------------------------------- > Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From zacharyweil <@t> yahoo.com Thu Jun 22 15:35:39 2006 From: zacharyweil <@t> yahoo.com (Zach Weil) Date: Thu Jun 22 15:35:45 2006 Subject: [Histonet] (no subject) Message-ID: <20060622203539.66105.qmail@web50605.mail.yahoo.com> We are trying to develop a model of adjuvant arthritis in dwarf hamsters that we use. Does anyone have a protocol for processing and staining small rodent (mouse) ankles for H and E in an arthritis model? Thanks, Zachary Weil --------------------------------- Want to be your own boss? Learn how on Yahoo! Small Business. From TJasper <@t> smdc.org Thu Jun 22 15:38:29 2006 From: TJasper <@t> smdc.org (Jasper, Thomas G.) Date: Thu Jun 22 15:38:18 2006 Subject: [Histonet] slicing paraffin embeded specimens Message-ID: <1A9F2A6C5762524799A816F1F09744CF1E0AF7@SCREECH.ntcampus.smdc.org> Hey John, What are you sectioning? 14 microns sounds awfully thick to me. I am in the clinical lab world so maybe you are working on some kind of special project or something that requires thicker sections. It is our standard here to cut at 4 microns or thinner. Some folks may disagree with me on this point, but most people I know in the world of clinical histology section at 4 or 5 microns, or less depending on tissue types and applications. One more suggestion for you John. I would bet if you contacted the local hospital in your town, someone would be willing to give you a very cursory tour/demo of pathology/histology services. I know these people are around, we are just a well hidden group of uniquely talented individuals. Hope this helps. Thomas Jasper Anatomic Pathology Supervisor SMDC Clinical Lab Duluth, MN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Campbell, John Thomas (UMR-Student) Sent: Thursday, June 22, 2006 1:49 PM To: histonet Subject: [Histonet] slicing paraffin embeded specimens I am just getting started in histology and I am having trouble keeping my slices from bunching up on the microtome. What can I do to keep this from happening? I am using a manual slicer, slicing the specimens into 14 microns thick slices. John Campbell Graduate Student University of Missouri Rolla Lab phone 573-341-4069 Cell phone 573-694-0596 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From dgaupp <@t> tulane.edu Thu Jun 22 16:37:05 2006 From: dgaupp <@t> tulane.edu (dgaupp@tulane.edu) Date: Thu Jun 22 16:37:12 2006 Subject: [Histonet] best cryostat? Message-ID: <1151012225.449b0d81c6fc2@webmail.tulane.edu> Histonetters: I would like to know what is the BEST cryostat on the market to buy for research purposes? Many Thanks, Dina D. Gaupp, B.S., M.T. Medical Research Specialist Center for Gene Therapy Tulane University Health Sciences Center JBJ Bldg/Rm 658, SL-99 1430 Tulane Avenue New Orleans, LA 70112 Lab: 504.988.1194 Fax: 504.988.7710 Email: dgaupp@tulane.edu From cfavara <@t> niaid.nih.gov Thu Jun 22 17:05:56 2006 From: cfavara <@t> niaid.nih.gov (Favara, Cynthia (NIH/NIAID) [E]) Date: Thu Jun 22 17:06:00 2006 Subject: [Histonet] best cryostat? In-Reply-To: <1151012225.449b0d81c6fc2@webmail.tulane.edu> Message-ID: I think this is dependant on what you are doing - but I would demo as many as possible and look fro one where the temperature of the stage can be controlled. Out of the cryostat loop but I seem to remember Alan Bright being very knowledgeable. c Cynthia Favara NIAID/NIH/RML/LPVD 903 South 4th Street Hamilton, MT 59840 406-363-9317 Disclaimer: The information in this e-mail and any of its attachments is confidential and may contain sensitive information. It should not be used by anyone who is not the original intended recipient. If you have received this e-mail in error please inform the sender and delete it from your mailbox or any other storage devices. National Institute of Allergy and Infectious Diseases shall not accept liability for any statements made that are sender's own and not expressly made on behalf of the NIAID by one of its representatives -----Original Message----- From: dgaupp@tulane.edu [mailto:dgaupp@tulane.edu] Sent: Thursday, June 22, 2006 2:37 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] best cryostat? Histonetters: I would like to know what is the BEST cryostat on the market to buy for research purposes? Many Thanks, Dina D. Gaupp, B.S., M.T. Medical Research Specialist Center for Gene Therapy Tulane University Health Sciences Center JBJ Bldg/Rm 658, SL-99 1430 Tulane Avenue New Orleans, LA 70112 Lab: 504.988.1194 Fax: 504.988.7710 Email: dgaupp@tulane.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dcrippen <@t> buckinstitute.org Thu Jun 22 18:39:12 2006 From: dcrippen <@t> buckinstitute.org (Danielle Crippen) Date: Thu Jun 22 18:39:15 2006 Subject: [Histonet] thawing and re-freezing cryosections Message-ID: Dearest Histo-experts, I have an investigator here who has injected mouse brains with a GFP construct. They've been perfused with saline and PFA, cryoprotected, and cryosectioned. But the investigator wants us only to perform IHC on those sections which contain GFP. In order to determine this, we'll have to air dry the slides. But I'm wondering how to store those slides which have been air dried but do not contain the GFP. Can they be stored like paraffin sections at this point (in boxes at RT)...can they be put back at -80? Any/all advice is VERY welcome!! Cheers, Danielle Crippen From a.tuck <@t> uq.edu.au Thu Jun 22 19:51:19 2006 From: a.tuck <@t> uq.edu.au (Mr Andrew Tuck) Date: Thu Jun 22 19:51:27 2006 Subject: [Histonet] Superfrost plus slides losing sections Message-ID: <113d7e0113a83d.113a83d113d7e0@uq.edu.au> Hi everybody I've had a problem with losing cryostat P0 mouse brain sections from superfrost plus slides during H&E staining. They are coming off in the xylene stage of the washes. The sections are allowed to dry for 24hrs in a laminar flow cabinet before washing. I did use an old box of slides (~ 12 months old) for these sections, so that may have something to do with it. I am re-testing using new slides. I thought these slides were more "sticky" than anything else. Has anybody had this problem ? Is there a way to prepare the slides to prevent this from occuring ? Any feedback would be appreciated. Thanks Andrew Tuck Research Assistant Queensland Centre for Schizophrenia Research School of Biomedical Sciences University of Queensland Australia Tel: 07 3346 2368 From lpwenk <@t> sbcglobal.net Thu Jun 22 19:55:33 2006 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Jun 22 19:55:41 2006 Subject: [Histonet] QIHC Exam In-Reply-To: Message-ID: <004b01c6965f$bbc53180$9e44dc45@HPPav2> According to the ASCP QIHC application form http://www.ascp.org/Certification/pdf/qihc.pdf The QIHC exam is 50 multiple choice questions, to be completed within 90 minutes. I haven't taken it, but I use these to teach my students about IHC: DAKO IHC Staining Methods Handbook http://www.dakousa.com/index/support/literature/literature_immunochemical_st aining_methods.htm Bancroft and Gamble "Theory and Practice of Histological Techniques" Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Thursday, June 22, 2006 2:46 PM To: Histonet (E-mail) Subject: [Histonet] QIHC Exam Howdy Histonetters, I was wondering if anyone out there just took the QIHC exam. Was it a timed exam? What was the best study guides? I would really appreciate any input. Cheers, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathololgy Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu From Jason.PALMER <@t> svhm.org.au Thu Jun 22 20:44:17 2006 From: Jason.PALMER <@t> svhm.org.au (PALMER Jason (SVHM)) Date: Thu Jun 22 20:44:26 2006 Subject: [Histonet] Lectin histochemistry for rabbit blood vessels Message-ID: Hello all. I have been asked to try and find a lectin that will allow labelling of rabbit blood vessels (in connective tissues). In our lab we have used Griffonia simplicifolia to label rat vessels, and Lycopersicon esculentum to label mouse vessels (both are biotinylated, from Vector). I can try these on some rat tissue, and I will do this, but I thought a quick question to histonet may be useful, if anyone can point me in the right direction re rabbit labelling. I know there is a book on Lectin Histochemistry (Brooks et al) available that Gayle Callis has recommended, and I am hoping to purchase a copy of this, but otherwise I have found it hard to locate basic information on lectin reactivity. Thanks for any responses, Jason Jason Palmer Bernard O'Brien Institute of Microsurgery 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4018 fax +61 3 9416 0926 email: jason.palmer@svhm.org.au Disclaimer : The contents of this e-mail including any attachments are intended only for the person or entity to which this e-mail is addressed and may contain confidential, privileged and/or commercially sensitive material. If you are not, or believe you may not be, the intended recipient, please advise the sender immediately by return e-mail, delete this e-mail and destroy any copies. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From barbara.bublava <@t> meduniwien.ac.at Fri Jun 23 04:27:47 2006 From: barbara.bublava <@t> meduniwien.ac.at (Barbara Bublava) Date: Fri Jun 23 04:27:56 2006 Subject: [Histonet] neutrophils In-Reply-To: <6.0.0.22.1.20060622104618.01b49450@gemini.msu.montana.edu> References: <0E394B648E5284478A6CCB78E5AFDA270171FE10@hpes1.HealthPartners.int> <449A4B6F.5040905@meduniwien.ac.at> <6.0.0.22.1.20060622090629.01b54e28@gemini.msu.montana.edu> <449AC20F.5040406@meduniwien.ac.at> <6.0.0.22.1.20060622104618.01b49450@gemini.msu.montana.edu> Message-ID: <449BB413.2070309@meduniwien.ac.at> Thank you Gayle, coming from you this means a lot to me and the results of my litte test will not only be true for every species. Every stain will suffer more or less... I?ll try to put some photos on the internet soon Greetings Barbara Bublava Gayle Callis wrote: > Barbara, > > This was very nicely set up and you proved your point. The same applies > for any species tissue in terms of fixation. My compliments on the > thoroughness of your fixation test/experiment. > > Thank you for elaborating on exactly what you did - > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 > 406 994-4303 (FAX) > > > From PKamalavenkatesh <@t> wockhardtin.com Fri Jun 23 06:30:47 2006 From: PKamalavenkatesh <@t> wockhardtin.com (PKamalavenkatesh@wockhardtin.com) Date: Fri Jun 23 06:23:41 2006 Subject: [Histonet] safranin o staining-rat cartilage-reg Message-ID: Dear histonetters, This is with regard to safranin o staining of proteoglycans of immature rat cartilage. I have followed the protocol from Gayle Callis. Unfortunately I couldn?t get any type of red color staining of proteoglycans in the articular cartilage. I am having doubt if all the proteoglycans got washed away. The joints are fixed in 10% neutral buffered formalin and then decalcified using 10 % EDTA solution for a week. Can anybody experiencing the same problem like this. In the literature, t was recommended that addition of cetylpyridinium chloride in the fixative will reduce the loss of proteoglycans during fixation and processing. If anybody having any sort of experience regarding this. Also can anybody provide me the Toluidine blue staining methodology for cartilage. Regards REGARDS Dr.P.Kamalavenkatesh Pre clinical safety assessment division New Drug Discovery- Biology Wockhardt Research Center India Please Visit our New Corporate Web Site www.wockhardt.com --------------------- Disclaimer ------------------------------------------------------------------------------ Information transmitted by this E-MAIL is proprietary to Wockhardt Ltd. and/ or its Group Companies and/or,its Customers and is intended for use only by the individual or entity to which it is addressed, and may contain information that is privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. --------------------------------------------------------------------------------------------------------------- From fran_lemons <@t> yahoo.com Fri Jun 23 07:05:52 2006 From: fran_lemons <@t> yahoo.com (Fran Walker) Date: Fri Jun 23 07:05:58 2006 Subject: [Histonet] Pen-fix Message-ID: <20060623120552.41859.qmail@web61314.mail.yahoo.com> Does anyone have a recipe for making "homemade" Pen-fix using 10% NBF and ethyl alcohol? We would rather not use straight formaldehyde if we can avoid it. Thanks in advance, Fran, Jackie and Ruben --------------------------------- Do you Yahoo!? Next-gen email? Have it all with the all-new Yahoo! Mail Beta. From kemlo.rogerson <@t> waht.swest.nhs.uk Fri Jun 23 07:21:44 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Jun 23 07:21:21 2006 Subject: [Histonet] Pen-fix Message-ID: Given your e-mail address that's not PC is it? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Each one sees what he carries in his heart. --Johann Wolfgang von Goethe This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From PIXLEYSK <@t> UCMAIL.UC.EDU Fri Jun 23 07:22:07 2006 From: PIXLEYSK <@t> UCMAIL.UC.EDU (Pixley, Sarah (pixleysk)) Date: Fri Jun 23 07:22:16 2006 Subject: [Histonet] Marking specimens through decalcification Message-ID: Dear Histonet: I will be putting rodent nose tissues in 10% formic acid to decalcify (after perfusion fixation in 4% paraformaldehyde). I need to mark the tissues to keep track of them. What can I use that will withstand the several days in formic acid? Each nose will be in a separate Shandon biopsy bag. Thanks Sarah Pixley Univ. Cincinnati From PIXLEYSK <@t> UCMAIL.UC.EDU Fri Jun 23 07:30:40 2006 From: PIXLEYSK <@t> UCMAIL.UC.EDU (Pixley, Sarah (pixleysk)) Date: Fri Jun 23 07:30:44 2006 Subject: [Histonet] Prussian Blue staining-thick brain sections Message-ID: Dear Histonet: Second message from me today, but different subject. I am staining sections (approx. 1 cm thick) of human brains, taken from the cadavers that were used by the first year medical students. I am staining them with Prussian Blue for use in the medical student neuroanatomy teaching labs. I am thinking of trying to do some fancier staining and our own, in house, plastination of the sections after staining. However, there are lots of methodological questions that come up, both in the staining and in the plastination. I have a fairly limited budget for this, hence the home plastination. If anyone has experience with this, would you be so kind as to email me separately so that we could have a discussion about this? THANK YOU! Sarah Pixley (sarah.pixley@uc.edu) Univ. Cincinnati From mcauliff <@t> umdnj.edu Fri Jun 23 07:58:16 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Jun 23 07:57:41 2006 Subject: [Histonet] Prussian Blue staining-thick brain sections In-Reply-To: References: Message-ID: <449BE568.7000801@umdnj.edu> Hi Sarah: Look up Stain Technology 50(2):87-91, 1975. I don't know if this would work for plastinated tissue. The references might be useful. Geoff (UC 1978, when the Anatomy dept.was Anatomy) Pixley, Sarah (pixleysk) wrote: > >Dear Histonet: >Second message from me today, but different subject. I am staining >sections (approx. 1 cm thick) of human brains, taken from the cadavers >that were used by the first year medical students. I am staining them >with Prussian Blue for use in the medical student neuroanatomy teaching >labs. I am thinking of trying to do some fancier staining and our own, >in house, plastination of the sections after staining. However, there >are lots of methodological questions that come up, both in the staining >and in the plastination. I have a fairly limited budget for this, hence >the home plastination. If anyone has experience with this, would you be >so kind as to email me separately so that we could have a discussion >about this? > >THANK YOU! > >Sarah Pixley (sarah.pixley@uc.edu) >Univ. Cincinnati > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From Laurie.Cote <@t> cytyc.com Fri Jun 23 08:09:06 2006 From: Laurie.Cote <@t> cytyc.com (Cote, Laurie) Date: Fri Jun 23 08:08:56 2006 Subject: [Histonet] Has anyone done a comprehensive study on fixatives? Message-ID: <3E76917CB874EF4DB6DF79DD5CEBC117115B0873@nahqmails9.cytyc.com> Has there ever been a comprehensive study done on fixatives? For instance, the differences between fixation with formalin, different kinds of alcohol or other fixatives? Specifically, is one fixative better for IHC? If a paper has been done and published, anyone know where it might be? I tried a pubmed search and was relatively unsuccessful. From jennifer.l.hofecker <@t> Vanderbilt.Edu Fri Jun 23 08:34:14 2006 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Fri Jun 23 08:36:52 2006 Subject: [Histonet] thawing and re-freezing cryosections References: Message-ID: <898D946569A27444B65667A49C074052852D82@mailbe06.mc.vanderbilt.edu> Danielle, What do you think you might have to do on these slides later? Are these just going to be kept "just in case" or do you have definte plans for them in the future? I may have some ideas depending on what will happen to them in the future. Have a great Friday! Jennifer Hofecker, HT (ASCP) Vanderbilt University Medical Center Division of Neuropathology (615) 343-0083 (615) 343-7089 fax ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Danielle Crippen Sent: Thu 6/22/2006 6:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] thawing and re-freezing cryosections Dearest Histo-experts, I have an investigator here who has injected mouse brains with a GFP construct. They've been perfused with saline and PFA, cryoprotected, and cryosectioned. But the investigator wants us only to perform IHC on those sections which contain GFP. In order to determine this, we'll have to air dry the slides. But I'm wondering how to store those slides which have been air dried but do not contain the GFP. Can they be stored like paraffin sections at this point (in boxes at RT)...can they be put back at -80? Any/all advice is VERY welcome!! Cheers, Danielle Crippen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From portera <@t> msu.edu Fri Jun 23 08:40:12 2006 From: portera <@t> msu.edu (Amy Porter) Date: Fri Jun 23 08:38:55 2006 Subject: [Histonet] Marking specimens through decalcification References: Message-ID: <000601c696ca$8d2e2690$8e7a0923@HistoJJ> I would suggest using tissue marking dye only on the muscle and tissue surrounding the turbinate. This would allow you to embed the samples ink up and not have the ink run into the nasal cavity. ----- Original Message ----- From: "Pixley, Sarah (pixleysk)" To: Sent: Friday, June 23, 2006 8:22 AM Subject: [Histonet] Marking specimens through decalcification Dear Histonet: I will be putting rodent nose tissues in 10% formic acid to decalcify (after perfusion fixation in 4% paraformaldehyde). I need to mark the tissues to keep track of them. What can I use that will withstand the several days in formic acid? Each nose will be in a separate Shandon biopsy bag. Thanks Sarah Pixley Univ. Cincinnati _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Malcolm.McCallum <@t> tamut.edu Fri Jun 23 08:41:56 2006 From: Malcolm.McCallum <@t> tamut.edu (Malcolm McCallum) Date: Fri Jun 23 08:43:18 2006 Subject: [Histonet] Has anyone done a comprehensive study on fixatives? References: <3E76917CB874EF4DB6DF79DD5CEBC117115B0873@nahqmails9.cytyc.com> Message-ID: I believe this kind of information was present in some of the old histotechnique textbooks. Whether they have done it recently to include modern fixatives I don't know, but it seems that companies producing this stuff would advertise the results if they are good! VISIT HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org A New Journal Published in Partnership with Partners in Amphibian and Reptile Conservaiton and the World Congress of Herpetology. Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-223-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Cote, Laurie Sent: Fri 6/23/2006 8:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Has anyone done a comprehensive study on fixatives? Has there ever been a comprehensive study done on fixatives? For instance, the differences between fixation with formalin, different kinds of alcohol or other fixatives? Specifically, is one fixative better for IHC? If a paper has been done and published, anyone know where it might be? I tried a pubmed search and was relatively unsuccessful. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Fri Jun 23 09:03:23 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Jun 23 09:02:48 2006 Subject: [Histonet] Has anyone done a comprehensive study on fixatives? In-Reply-To: <3E76917CB874EF4DB6DF79DD5CEBC117115B0873@nahqmails9.cytyc.com> References: <3E76917CB874EF4DB6DF79DD5CEBC117115B0873@nahqmails9.cytyc.com> Message-ID: <449BF4AB.3060100@umdnj.edu> Lots of papers were published in the 1980's in various histochemical journals. Try searching PubMed or a recent textbook on IHC. Geoff Cote, Laurie wrote: >Has there ever been a comprehensive study done on fixatives? For >instance, the differences between fixation with formalin, different >kinds of alcohol or other fixatives? Specifically, is one fixative >better for IHC? If a paper has been done and published, anyone know >where it might be? I tried a pubmed search and was relatively >unsuccessful. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From pmcardle <@t> ebsciences.com Fri Jun 23 09:22:43 2006 From: pmcardle <@t> ebsciences.com (Phil McArdle) Date: Fri Jun 23 09:22:52 2006 Subject: [Histonet] Has anyone done a comprehensive study on fixatives? Message-ID: <449BF933.7020006@ebsciences.com> Hello: If you'll forgive a "vendor" response, Dick Dapson at Anatech has some very good comparisons of the various fixative types (alcohol, formalin, glyoxal), and publishes "The Innovator," a newsletter with some information you may find useful: http://www.anatechltdusa.com/Innovators/InnList.html Yes, Anatech does sell glyoxal fixatives, but the information is worth considering. Best regards, Phil McArdle -- Phil McArdle Microwave Product Manager Energy Beam Sciences, Inc. Tel: 800.992.9037 x 341 Fax: 860.653.0422 PMcardle@ebsciences.com www.ebsciences.com "ADDING BRILLIANCE TO YOUR VISION" "I hate quotations. Tell me what you know." Ralph Waldo Emerson From ROrr <@t> enh.org Fri Jun 23 09:48:58 2006 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Fri Jun 23 09:49:06 2006 Subject: [Histonet] Pressure Cooker Message-ID: HI Dr. Raff, I use the Biocare Medical Decloaker and it is exactly the same as your Pascal. During the heat up phase you'll notice the lid and rubber gasket fit together a bit loosely. Until there is enough heat to cause a pressure build up, some steam will escape. You'll notice that once you have achieved a temp of 120 to 125'C the steaming and hissing slows down and even stops. That's because the chamber is now pressurized and the gasket has made a tight seal. Just make sure the pressure release that sits on the lid sits evenly when you close the lid. If that pressure release is crooked, that indicates you don't have the lid sealed correctly. I hope this helps. Becky Orr CLA,HT(ASCP) Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 B822019850@UPLAB01.uplab.local> Content-Type: text/plain; charset="us-ascii" Hi all: We are using the PASCAL pressure cooker from DAKO for HIER. We have noticed on occasion steam coming out of the edges (not through the pressure release valve). Our rep says this is normal. Do others have the same experience? Also, we are still looking for a part time histotech. Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 ------------------------------ From PMonfils <@t> Lifespan.org Fri Jun 23 10:22:33 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Jun 23 10:22:39 2006 Subject: [Histonet] Marking specimens through decalcification Message-ID: <09C945920A6B654199F7A58A1D7D1FDE01717744@lsexch.lsmaster.lifespan.org> I use black India ink for marking tissues, available from any art supply or stationery store. It stays on the surface of the tissue (doesn't tend to penetrate tissue to any extent), and withstands just about any chemical treatment (the colorant is carbon particles - pretty inert). > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > Pixley, Sarah (pixleysk) > Sent: Friday, June 23, 2006 5:22 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Marking specimens through decalcification > > > Dear Histonet: > I will be putting rodent nose tissues in 10% formic acid to decalcify > (after perfusion fixation in 4% paraformaldehyde). I need to mark the > tissues to keep track of them. What can I use that will withstand the > several days in formic acid? Each nose will be in a separate Shandon > biopsy bag. > > Thanks > Sarah Pixley > Univ. Cincinnati > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From gcallis <@t> montana.edu Fri Jun 23 10:31:07 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Jun 23 10:31:20 2006 Subject: [Histonet] Lectin histochemistry for rabbit blood vessels In-Reply-To: References: Message-ID: <6.0.0.22.1.20060623085851.01b780f0@gemini.msu.montana.edu> Jason, Have you tried either lectin of these on rabbit just to see if they work ? Another superb sources of lectins EY Laboratories Lectin and Lectin Conjugates, you should get this booklet if possible. Access this publication, Spicer SS, Schulte BA Diversity of cell glycoconjugates shown histochemically: a perspective. J Histochemistry Cytochemistry 40(1):1-38, 1992. and is an excellent review of lectins. Griffonia simplicifolia is also known as Bandeiraea simpicifolia and has 7 differerent groups, the name difference may help with a literature search. PUBMED and elsewhere. Be sure to do a search in J Histochemistry Cytochemistry too My copy of Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From asmith <@t> mail.barry.edu Fri Jun 23 10:33:12 2006 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Jun 23 10:33:20 2006 Subject: [Histonet] threshhold for antibody binding Message-ID: <7DBCCC1FBC77C94F99F920D0CA6400B61E3F96@exchsrv01.barrynet.barry.edu> We have an antibody that binds well at a 1:75 dilution, but does not appear to bind at all when the dilution is 1:85. Has anyone noticed a similar phenomenon: a sharp dilution threshhold below which the antibody works well and above which it does not work at all? Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From Barry.R.Rittman <@t> uth.tmc.edu Fri Jun 23 10:37:20 2006 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Fri Jun 23 10:37:24 2006 Subject: [Histonet] Lectin histochemistry for rabbit blood vessels Message-ID: Jason Two major sources that I know of. 1. The Lectins by Sharon and Lis. While this is an older text (1986) it has a very complete listing re lectin specificities and inhibitors. 2. Vector Labs. As we are advised not to send attachment for fear of clogging the system I will email the relevant pages to me separately. Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of PALMER Jason (SVHM) Sent: Thursday, June 22, 2006 8:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Lectin histochemistry for rabbit blood vessels Hello all. I have been asked to try and find a lectin that will allow labelling of rabbit blood vessels (in connective tissues). In our lab we have used Griffonia simplicifolia to label rat vessels, and Lycopersicon esculentum to label mouse vessels (both are biotinylated, from Vector). I can try these on some rat tissue, and I will do this, but I thought a quick question to histonet may be useful, if anyone can point me in the right direction re rabbit labelling. I know there is a book on Lectin Histochemistry (Brooks et al) available that Gayle Callis has recommended, and I am hoping to purchase a copy of this, but otherwise I have found it hard to locate basic information on lectin reactivity. Thanks for any responses, Jason Jason Palmer Bernard O'Brien Institute of Microsurgery 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4018 fax +61 3 9416 0926 email: jason.palmer@svhm.org.au Disclaimer : The contents of this e-mail including any attachments are intended only for the person or entity to which this e-mail is addressed and may contain confidential, privileged and/or commercially sensitive material. If you are not, or believe you may not be, the intended recipient, please advise the sender immediately by return e-mail, delete this e-mail and destroy any copies. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Fri Jun 23 10:56:06 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Jun 23 10:56:14 2006 Subject: [Histonet] Marking specimens through decalcification In-Reply-To: References: Message-ID: <6.0.0.22.1.20060623095037.01b75d00@gemini.msu.montana.edu> Do you mean an accession number for each nose? We mark the nylon bag itself with soft lead pencil and also put a thick paper tag with ID# written in pencil inside the bag with the sample. Be sure you close the bag with string, or things turned upside down get mixed up, we use the string to hang the bag in a huge container of 10% formic acid, and stir to get rid of bubbles (CO2, a results of decalcification). Suspension aids in faster, even decalcification. At 06:22 AM 6/23/2006, you wrote: > >Dear Histonet: >I will be putting rodent nose tissues in 10% formic acid to decalcify >(after perfusion fixation in 4% paraformaldehyde). I need to mark the >tissues to keep track of them. What can I use that will withstand the >several days in formic acid? Each nose will be in a separate Shandon >biopsy bag. > >Thanks >Sarah Pixley >Univ. Cincinnati > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From celebrej <@t> HHSC.CA Fri Jun 23 10:56:37 2006 From: celebrej <@t> HHSC.CA (Celebre Julia) Date: Fri Jun 23 10:56:48 2006 Subject: [Histonet] Melanin bleach Message-ID: <0B3BF9D810C7094FA34B17DD145C3D5E015E9B31@ipemail01.hhsc.ca> Hello all you histo experts, here's a question for you. We are having a discussion regarding melanin, whether after the bleaching step should you follow thru with the Masson Fontana silver method or not. Some say yes... some say no... Those of us that say yes feel that that's how one can tell if it's melanin or not.. Those that say no, feel that it's a waste of time because if the pigment is no longer in the bleached section obviously it must've been melanin.. There's also some that feel if you run the bleached section thru the silver method, you may get precipitate, or false positives?? Opinions anyone??? Julia Celebre MLT Anatomic Pathology Hamilton General Hospital 905-527-0271 ext 46179 email: celebrej@hhsc.ca This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. From KParker <@t> mail.nih.gov Fri Jun 23 11:05:39 2006 From: KParker <@t> mail.nih.gov (Tuttle, Kimberly (NIH/NCI) [E]) Date: Fri Jun 23 11:05:44 2006 Subject: [Histonet] Has anyone done a comprehensive study on fixatives? In-Reply-To: <3E76917CB874EF4DB6DF79DD5CEBC117115B0873@nahqmails9.cytyc.com> Message-ID: We actually have our summer students working on that for their research project this summer. They will do a poster session at the end of the summer. Kimberly C. Tuttle H.T.,(ASCP) NCI Center for Cancer Research Tissue Array Research Program http://resresources.nci.nih.gov/tarp -----Original Message----- From: Cote, Laurie [mailto:Laurie.Cote@cytyc.com] Sent: Friday, June 23, 2006 8:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Has anyone done a comprehensive study on fixatives? Has there ever been a comprehensive study done on fixatives? For instance, the differences between fixation with formalin, different kinds of alcohol or other fixatives? Specifically, is one fixative better for IHC? If a paper has been done and published, anyone know where it might be? I tried a pubmed search and was relatively unsuccessful. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Jun 23 11:13:18 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 23 11:13:24 2006 Subject: [Histonet] Melanin bleach In-Reply-To: <0B3BF9D810C7094FA34B17DD145C3D5E015E9B31@ipemail01.hhsc.ca> Message-ID: <20060623161318.57226.qmail@web61214.mail.yahoo.com> Julia: The reasoning behind bleaching one section and continue with Fontana Masson, is the same as digesting with diastase a section and doing PAS afterwards. The diastase digested, as well as the melanin bleached section, would be known "negative" controls, to be run at the same time as a known positive control with the case. Hope this will help you! Ren? J. Celebre Julia wrote: Hello all you histo experts, here's a question for you. We are having a discussion regarding melanin, whether after the bleaching step should you follow thru with the Masson Fontana silver method or not. Some say yes... some say no... Those of us that say yes feel that that's how one can tell if it's melanin or not.. Those that say no, feel that it's a waste of time because if the pigment is no longer in the bleached section obviously it must've been melanin.. There's also some that feel if you run the bleached section thru the silver method, you may get precipitate, or false positives?? Opinions anyone??? Julia Celebre MLT Anatomic Pathology Hamilton General Hospital 905-527-0271 ext 46179 email: celebrej@hhsc.ca This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Ring'em or ping'em. Make PC-to-phone calls as low as 1?/min with Yahoo! Messenger with Voice. From mtitford <@t> aol.com Fri Jun 23 11:50:16 2006 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Fri Jun 23 11:50:29 2006 Subject: [Histonet] Fixative studies Message-ID: <8C864FC3D1394F5-17A8-8118@mblk-r21.sysops.aol.com> Laurie Cote asks about studies comparing fixatives. There have been several recently, i.e. over the last few years. A useful one was by Prento & Lyon "Commercial Formalin Substitutes for Histopathology" Biotech Histochem 1997 72: 273 - 282 Mike Titford USA Pathology Mobile AL USA ________________________________________________________________________ Check out AOL.com today. Breaking news, video search, pictures, email and IM. All on demand. Always Free. From mcauliff <@t> umdnj.edu Fri Jun 23 12:19:35 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Jun 23 12:20:11 2006 Subject: [Histonet] safranin o staining-rat cartilage-reg In-Reply-To: References: Message-ID: <449C22A7.8010206@umdnj.edu> Routine formalin fixation will not maximize the preservation of cartilage proteoglycans. CPC or alcohol fixation will be better. Before proceeding, consult the papers listed below by Shepard and Mitchell. J. Histochem. Cytochem. 24(5):621-629, 1976. J. Ultrastruc. Res. 54-451-460, 1976. Histochemistry 70:107-114, 1981. Anat. Rec. 187(4):463-476. also J. Elec. Micros. Tech. 5:17-44, 1987 by Thomopoulos et al. Geoff PKamalavenkatesh@wockhardtin.com wrote: >Dear histonetters, > >This is with regard to safranin o staining of proteoglycans of immature rat >cartilage. I have followed the protocol from Gayle Callis. Unfortunately I >couldn?t get any type of red color staining of proteoglycans in the >articular cartilage. I am having doubt if all the proteoglycans got washed >away. The joints are fixed in 10% neutral buffered formalin and then >decalcified using 10 % EDTA solution for a week. Can anybody experiencing >the same problem like this. In the literature, t was recommended that >addition of cetylpyridinium chloride in the fixative will reduce the loss >of proteoglycans during fixation and processing. If anybody having any sort >of experience regarding this. Also can anybody provide me the Toluidine >blue staining methodology for cartilage. > >Regards >REGARDS >Dr.P.Kamalavenkatesh >Pre clinical safety assessment division >New Drug Discovery- Biology >Wockhardt Research Center >India > >Please Visit our New Corporate Web Site www.wockhardt.com >--------------------- Disclaimer ------------------------------------------------------------------------------ >Information transmitted by this E-MAIL is proprietary to Wockhardt Ltd. and/ or its Group Companies >and/or,its Customers and is intended for use only by the individual or entity to which it is addressed, >and may contain information that is privileged, confidential or exempt from disclosure under applicable law. >If you are not the intended recipient or it appears that this mail has been forwarded to you without proper >authority, you are notified that any use or dissemination of this information in any manner is strictly >prohibited. In such cases, please delete this mail from your records. >--------------------------------------------------------------------------------------------------------------- > > >------------------------------------------------------------------------ > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From SHARON.OSBORN <@t> SPCORP.COM Fri Jun 23 12:21:22 2006 From: SHARON.OSBORN <@t> SPCORP.COM (Osborn, Sharon) Date: Fri Jun 23 12:21:27 2006 Subject: [Histonet] HOPE-FIXATION Message-ID: <9A919A5D70313A4D9C56A025710874080C7311@kenmsg40.us.schp.com> Histonetters! Do any of you have experience with the HOPE fixative from DCS, novative Diagnostik-Systeme from Hamburg, Germany? One of our researchers brought the article to show me and ask if we can use only the HOPE I for frozen tissues and do OCT blocks, etc. The HOPE II if equal parts of the HOPE I and ice cold acetone to do dehydration processing into low tem. paraffin for paraffin embedding. HOPE is Hepes-Glutamic Acid Buffer Mediated Organic Solvent Protection Effect. It does not kill any bugs, etc. It does not completely denature structural proteins, enzymes and nuclec acids. Thanks, Sharon Osborn B.S. HT(ASCP)CT DNAX, Schering-Plough BioPharma Palo, Alto, CA 650.496.6539 ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From langxingpan <@t> sbcglobal.net Fri Jun 23 12:47:30 2006 From: langxingpan <@t> sbcglobal.net (Langxing Pan) Date: Fri Jun 23 12:47:40 2006 Subject: [Histonet] RE: multi-tissue blocks for IHC Message-ID: Dear Theresa, We are providing IHC multi-tissue control blocks and sections for laboratory use either in ready-made forms or customized forms. Our ready-made blocks or sections include Universal IHC control covering ~90% of the markers currently used in routine IHC, breast hormone receptor IHC controls (http://www.pantomics.com/products/products_arrays.htm#IHCcontrolarray). We are currently developing and validating many other control panels such as multi-tissue controls for cyclin D1, bcl-2, bcl-6,ALK, kappa/ lambda light chains and infectious pathogens. I can send you some trial samples, if you like. Regards, Langxing Pan, M.D., Ph.D. Pantomics, Inc. 457 Mariposa Street San Francisco, CA 94107 Direct: 1-415-863-2380 langxingpan@pantomics.com www.pantomics.com Message: 7 Date: Thu, 22 Jun 2006 15:30:09 -0400 From: "Theresa Rohr" Subject: [Histonet] Multitissue blocks To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain Hi out there, I was wondering if any of you order multi-tissue blocks for IHC and if so where from? Thanks for the information. Theresa Rohr, Nyack Hospital NY rohrt@nyackhospital.org Theresa Rohr, BA, HT(ASCP) Section Head, Histology Nyack Hospital 160 North Midland Avenue Nyack, New York 10960 phone 845-348-2276 fax 845-348-8430 From portera <@t> msu.edu Fri Jun 23 13:31:40 2006 From: portera <@t> msu.edu (Amy Porter) Date: Fri Jun 23 13:30:24 2006 Subject: [Histonet] HOPE-FIXATION References: <9A919A5D70313A4D9C56A025710874080C7311@kenmsg40.us.schp.com> Message-ID: <006101c696f3$44c41bc0$8e7a0923@HistoJJ> Sharon - One of our clients approached us about doing this fixative as well. We however do not have the staffing or work hours to be able to complete the process. They did the processing portion in their laboratory then delivered it to us when ready to have embedded in paraffin. I have never heard back from them how successful the results were. ----- Original Message ----- From: "Osborn, Sharon" To: Sent: Friday, June 23, 2006 1:21 PM Subject: [Histonet] HOPE-FIXATION Histonetters! Do any of you have experience with the HOPE fixative from DCS, novative Diagnostik-Systeme from Hamburg, Germany? One of our researchers brought the article to show me and ask if we can use only the HOPE I for frozen tissues and do OCT blocks, etc. The HOPE II if equal parts of the HOPE I and ice cold acetone to do dehydration processing into low tem. paraffin for paraffin embedding. HOPE is Hepes-Glutamic Acid Buffer Mediated Organic Solvent Protection Effect. It does not kill any bugs, etc. It does not completely denature structural proteins, enzymes and nuclec acids. Thanks, Sharon Osborn B.S. HT(ASCP)CT DNAX, Schering-Plough BioPharma Palo, Alto, CA 650.496.6539 ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PIXLEYSK <@t> UCMAIL.UC.EDU Fri Jun 23 14:05:45 2006 From: PIXLEYSK <@t> UCMAIL.UC.EDU (Pixley, Sarah (pixleysk)) Date: Fri Jun 23 14:05:50 2006 Subject: [Histonet] Decalcification and marking Message-ID: Dear Gayle: You asked: "Do you mean an accession number for each nose?" I suppose the answer is yes. We do research not clinical work and we use ID# not accession numbers. Thanks for the hints. We are going to use plastic paper clips to keep the bag closed. I have had some of the plastic paper clips in formic acid for several weeks now and they don't look like they are dissolving. We will weigh them and see if they lost weight. Sarah Pixley From PMonfils <@t> Lifespan.org Fri Jun 23 14:14:19 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Jun 23 14:14:26 2006 Subject: [Histonet] Decalcification and marking Message-ID: <09C945920A6B654199F7A58A1D7D1FDE01717745@lsexch.lsmaster.lifespan.org> Plastic fasteners sound good during decalcification, and I'm sure what I am about to say is obvious, but I would replace them with something else before processing. Or at least test them against your clearing agent. I staple the bags for processing. I don't try to remove the staples after processing, just cut the bags open with scissors. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > Pixley, Sarah (pixleysk) > Sent: Friday, June 23, 2006 12:05 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Decalcification and marking > > Dear Gayle: > You asked: "Do you mean an accession number for each nose?" I suppose > the answer is yes. We do research not clinical work and we use ID# not > accession numbers. > > Thanks for the hints. We are going to use plastic paper clips to keep > the bag closed. I have had some of the plastic paper clips in formic > acid for several weeks now and they don't look like they are dissolving. > We will weigh them and see if they lost weight. > > Sarah Pixley > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From rjbuesa <@t> yahoo.com Fri Jun 23 14:15:09 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jun 23 14:15:16 2006 Subject: [Histonet] threshhold for antibody binding In-Reply-To: <7DBCCC1FBC77C94F99F920D0CA6400B61E3F96@exchsrv01.barrynet.barry.edu> Message-ID: <20060623191509.19569.qmail@web61223.mail.yahoo.com> Allen: I had never experienced such a shart "stain-to-not at all" gradient. When diluted below the optimal ratio what I always found was weak staining, but not "no bind at all". I do not know why this could happen. Ren? J. "Smith, Allen" wrote: We have an antibody that binds well at a 1:75 dilution, but does not appear to bind at all when the dilution is 1:85. Has anyone noticed a similar phenomenon: a sharp dilution threshhold below which the antibody works well and above which it does not work at all? Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Next-gen email? Have it all with the all-new Yahoo! Mail Beta. From godsgirlnow <@t> msn.com Fri Jun 23 14:38:13 2006 From: godsgirlnow <@t> msn.com (Roxanne Soto) Date: Fri Jun 23 14:38:19 2006 Subject: [Histonet] Tampa Florida jobs In-Reply-To: Message-ID: We are looking for high quaility technologists here in the Tampa area (right across the street from Busch Gardens)....please contact me Roxanne Soto Clinical Supervisor Physicians RightPath Tampa ______________________________________________________________ From: "Mary" To: Subject: [Histonet] Tampa Florida jobs Date: Fri, 16 Jun 2006 23:26:38 -0400 >I, as several other techs are wondering what jobs may be available in the Tampa Florida area. Please send any info available. > >Thank you for your time, > >Marysia >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barry.R.Rittman <@t> uth.tmc.edu Fri Jun 23 15:03:30 2006 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Fri Jun 23 15:03:33 2006 Subject: [Histonet] Fixative studies Message-ID: Some suggestions. This is a short list. Barry 1. Mann, Gustav 1902. Physiological Histology. Methods and Theory. Oxford at the Clarendon Press. This text deals in detail with methods in histology and for fixation with the history and rationale behind using various chemical agents as fixatives. 2. Pearse A.G.E. The Chemistry and practice of Fixation. Chapter 5 pages 97 -158. In Histochemistry Theoretical and Applied vol 1. 4th edition. Preparative and Optical Techniques. 1980. Excellent description of fixation chemistry and extensive list of references. vol. 2. Analytical techniques 1985. vol 3. Enzyme Histochemistry 1991. Churchill Livingstone, London. 3. Arnold M.M., Srivastava S., Fredenburgh J., Stockyard C.R. and Myers R.B. 1996. Effects of fixation and tissue processing on immunohistochemical demonstration of specific antigens. Biotechnology & Histochemistry 71: 224-230. 4. Beckstead J.H. 1994. A Simple Technique for Preservation of Fixative-sensitive Antigens in Paraffin-embedded Tissues: J. Histochem. Cytochem. 43: 345. 5. Isaam Eltoum, Jerry Fredenburgh, Russel B. Myers and William Grizzle. 2001. Introduction to the theory and Practice of Fixation of Tissues. J. Histotechnology 24 #3 173- 190. 6. Hopwood D. 1985. Cell and tissue fixation, 1972 - 1982. Histochemical Journal. 17: 389-442 7. Prento P and Lyon H. 1997. Commercial Formalin Substitutes for Histopathology. Biotechnic & Histochemistry. 72 (5). 272-282. From dgaupp <@t> tulane.edu Fri Jun 23 18:19:40 2006 From: dgaupp <@t> tulane.edu (dgaupp@tulane.edu) Date: Fri Jun 23 18:19:47 2006 Subject: [Histonet] tissue separating from OCT Message-ID: <1151104780.449c770cb253d@webmail.tulane.edu> Hello Histonetters: I am having difficulty sectioning this mouse brain. The tissue is separating from OCT upon sectioning. Let me give you a brief background of how the brain was processed. Perfused with 4%PFA, fixed overnight at 4C(same fix), next day rinsed with 1XPBS, then serially cryoproctected overnight with 20%, 30%, 40% sucrose/1XPBS - tissue sunk every time. Rinsed tissue from the sucrose with 1XPBS, blot dry, then flash froze in OCT, immersed in isopentane @ -80C. I am trying to section at 10um. I've done this procedure before, many, many times, never had problem trying to get a nice section. I am about to pull my hair out!!! What's the point in putting a tissue that has wrinkles on a slide. I don't know what my problem is! I've adjusted the angle on the cryostat & adjusted the temperature, changed blades, changed the position of the tissue to make almost a full circle(cryostat specimen holder). Can someone in histoworld help with this frustration? I thought I was just having a bad cutting day, but this is now carried on for over a week. I need to section the entire brain - ever piece is precious to me. Helpless, Dina Dina D. Gaupp, B.S., M.T. Medical Research Specialist Center for Gene Therapy Tulane University Health Sciences Center JBJ Bldg/Rm 658, SL-99 1430 Tulane Avenue New Orleans, LA 70112 Lab: 504.988.1194 Fax: 504.988.7710 Email: dgaupp@tulane.edu From lpwenk <@t> sbcglobal.net Sat Jun 24 01:35:14 2006 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Sat Jun 24 01:35:23 2006 Subject: [Histonet] Has anyone done a comprehensive study on fixatives? In-Reply-To: <3E76917CB874EF4DB6DF79DD5CEBC117115B0873@nahqmails9.cytyc.com> Message-ID: <001601c69758$5a268d30$b7a14d44@HPPav2> I don't have the issue in front of me, so maybe a Histonetter can supply more information. A couple of years ago, the NSH Journal of Histotechnology published a special September issue, with all the articles related to fixation. There were some good charts in it, as to what type of fixative fixes what components. Contact NSH 301-262-6221, and see if they have any old issues you could buy. (The Journal archive web pages are still under construction on the NSH web page.) Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cote, Laurie Sent: Friday, June 23, 2006 9:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Has anyone done a comprehensive study on fixatives? Has there ever been a comprehensive study done on fixatives? For instance, the differences between fixation with formalin, different kinds of alcohol or other fixatives? Specifically, is one fixative better for IHC? If a paper has been done and published, anyone know where it might be? I tried a pubmed search and was relatively unsuccessful. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Barry.R.Rittman <@t> uth.tmc.edu Sat Jun 24 07:52:26 2006 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Sat Jun 24 07:52:16 2006 Subject: [Histonet] Has anyone done a comprehensive study on fixatives? Message-ID: I think that the problem with comparing fixatives is that there are so many thousands of formulae and processes in the literature that it is impossible for the newcomer to know where to start. Everyone has their favorite technique but often with their mown varaitions to the original. The following all play a role in the final image: 1. An individual fixative will give variable results with different tissues and tissue components. 2. Most laboartories will have different sizes and volumes of tissues. 3. Different processing techniques and times are used. 4. There is the question of secondary fixation, if not deliberate at least during the subsequent processing. 5.If wax processing then there maty be different reagents used for removing wax etc. 6.Different images will result sometimes with even slight variations in the staining, histochemial or immunohistochemical techniques. 7.Not all reagent do what the supplies states, and this appears at least to me to be especially true with immunohistochemistry reagents. 8. Sometimes histotechs, for a variety of reasons do not use adequte controls for specificity of reactions. 9. A positive or negative result may be in the eye of the beholder whether histotech or pathologist. 10. Many individuals who write to Histonet are using tissues from different species and it may not be possible to use the same technique for all species, whether this is regarding fixation or processing or staining. I feel that the most important criteria are conisitency and controls. Any publications regarding fixation should include not only the results after fixationn but also after procesing. If you want to compare fixatives you are best to use publications as a starting point and thedo a trial run yourself with the tissues you want to use. First decide precisely what you wish to finally demonstrate. You should include some different times and keep complete notes about temperature, concentrations etc. at all steps including, fixation, processing etc. Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Lee & Peggy Wenk Sent: Sat 6/24/2006 1:35 AM To: 'Cote, Laurie'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Has anyone done a comprehensive study on fixatives? I don't have the issue in front of me, so maybe a Histonetter can supply more information. A couple of years ago, the NSH Journal of Histotechnology published a special September issue, with all the articles related to fixation. There were some good charts in it, as to what type of fixative fixes what components. Contact NSH 301-262-6221, and see if they have any old issues you could buy. (The Journal archive web pages are still under construction on the NSH web page.) Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cote, Laurie Sent: Friday, June 23, 2006 9:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Has anyone done a comprehensive study on fixatives? Has there ever been a comprehensive study done on fixatives? For instance, the differences between fixation with formalin, different kinds of alcohol or other fixatives? Specifically, is one fixative better for IHC? If a paper has been done and published, anyone know where it might be? I tried a pubmed search and was relatively unsuccessful. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pamm <@t> hlclab.com Sat Jun 24 23:16:21 2006 From: pamm <@t> hlclab.com (Pam Mara) Date: Sat Jun 24 23:18:20 2006 Subject: [Histonet] salary for lab assistants In-Reply-To: <200606240952608.SM01140@swlx162.swmed.edu> Message-ID: <200606242110817.SM01140@juanital> I have a question. We have a lab assistant here who does the "cassetting" of tissue specimens. She isn't a PA and she doesn't really gross in any large specimens. We mainly have GI specimens and biopsies, which she weighs/measures and puts into cassettes. She also helps with staining, coverslipping, etc. I was just wondering what is a reasonable salary for someone who performs these tasks? Does anyone else work with a lab assistant that performs similar tasks? Thanks for the info, Pam Mara -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Saturday, June 24, 2006 9:52 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 31, Issue 36 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." From rjbuesa <@t> yahoo.com Sun Jun 25 08:37:57 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Jun 25 09:38:03 2006 Subject: [Histonet] salary for lab assistants In-Reply-To: <200606242110817.SM01140@juanital> Message-ID: <20060625133757.34510.qmail@web61215.mail.yahoo.com> Pam: For your description your assistant works as a histotech although she may or may not have "academic qualifications". You have to consider also that initially histotechs were trained "on the job" and later on were "grandfathered" at licensure time (perhaps this is one of the reasons why historically histotechs are in lower echelon of clinical lab salary scale, but that is another subject to talk about!). I do not think that you should ask about lab aides paymen rates when your aide is performing tasks above those of that job position. As a lab supervisor/manager it should be part of your mission that of assuring that the employees you supervise are treated fairly. Having said all that I think that you should get for her a salary that reflects her tasks, not those usually corresponding for a job level. It is my opinion that she shoudl be paid, at least, at the histotech entry level. Hope this will help you! Ren? J. Pam Mara wrote: I have a question. We have a lab assistant here who does the "cassetting" of tissue specimens. She isn't a PA and she doesn't really gross in any large specimens. We mainly have GI specimens and biopsies, which she weighs/measures and puts into cassettes. She also helps with staining, coverslipping, etc. I was just wondering what is a reasonable salary for someone who performs these tasks? Does anyone else work with a lab assistant that performs similar tasks? Thanks for the info, Pam Mara -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Saturday, June 24, 2006 9:52 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 31, Issue 36 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Want to be your own boss? Learn how on Yahoo! Small Business. From Pathrm35 <@t> adelphia.net Sun Jun 25 11:21:58 2006 From: Pathrm35 <@t> adelphia.net (Ron Martin) Date: Sun Jun 25 11:19:58 2006 Subject: [Histonet] salary for lab assistants In-Reply-To: <20060625133757.34510.qmail@web61215.mail.yahoo.com> Message-ID: <000301c69873$7bd44e10$2ea2ac45@D6WRV2B1> Should this lab assistant be "cassetting" at all? This sounds like a grossing tech to me and should fall under CLIA regs for high complex testing with the appropriate education/training. If this person isn't qualified should he/she be performing these duties at all? Ron Martin -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Sunday, June 25, 2006 9:38 AM To: Pam Mara; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] salary for lab assistants Pam: For your description your assistant works as a histotech although she may or may not have "academic qualifications". You have to consider also that initially histotechs were trained "on the job" and later on were "grandfathered" at licensure time (perhaps this is one of the reasons why historically histotechs are in lower echelon of clinical lab salary scale, but that is another subject to talk about!). I do not think that you should ask about lab aides paymen rates when your aide is performing tasks above those of that job position. As a lab supervisor/manager it should be part of your mission that of assuring that the employees you supervise are treated fairly. Having said all that I think that you should get for her a salary that reflects her tasks, not those usually corresponding for a job level. It is my opinion that she shoudl be paid, at least, at the histotech entry level. Hope this will help you! Ren? J. Pam Mara wrote: I have a question. We have a lab assistant here who does the "cassetting" of tissue specimens. She isn't a PA and she doesn't really gross in any large specimens. We mainly have GI specimens and biopsies, which she weighs/measures and puts into cassettes. She also helps with staining, coverslipping, etc. I was just wondering what is a reasonable salary for someone who performs these tasks? Does anyone else work with a lab assistant that performs similar tasks? Thanks for the info, Pam Mara -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Saturday, June 24, 2006 9:52 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 31, Issue 36 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Want to be your own boss? Learn how on Yahoo! Small Business. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Godsgalnow <@t> aol.com Sun Jun 25 14:21:40 2006 From: Godsgalnow <@t> aol.com (Godsgalnow@aol.com) Date: Sun Jun 25 14:21:46 2006 Subject: [Histonet] salary for lab assistants Message-ID: <533.1285966.31d03c44@aol.com> We pay ours from $11 - $14, I guess it depends on where you work. Roxanne Soto Clinical Lab Supervisor Physicians RightPath Tampa, Florida From Eric <@t> ategra.com Sun Jun 25 20:04:09 2006 From: Eric <@t> ategra.com (Eric Dye (ext 223)) Date: Sun Jun 25 20:17:59 2006 Subject: [Histonet] Histology Openings Message-ID: Hi - Below is an updated listing of Histology permanent and temporary jobs I have throughout the country. If you are interested in any of the Histology jobs listed below or anywhere else - please call me at 800-466-9919 ext 223 or cell - 407-756-5507. Most Histo Tech j o b s are permanent full-time dayshift. Here are some of my Newest Histology Jobs: ------------------------------------------------------ Virigina - HistoTech - perm Boston Mass - HistoTech - one Senior HistoTech, One not so Senior Histotech - days and early AMS Massachusetts (North of Boston) - perm - Bench Histotech Ohio (Central) - one temp and one perm - Bench Histotech West Virginia - perm - Bench Histotech/ Supervisor Minnesota (Twin Cities area - perm - Bench Histotech - Biotech/research Southeast Florida - temp - HistoTech Southwest Florida - perm - Bench Histotech/ Supervisor Florida, West Coast - both temp & perm openings- Bench Histotech Pennsylvania, Pittsburgh - perm - Bench HistoTech - day Virginia/D.C. Beltway East - Fairfax Area - Perm, Histo Tech / Histo Supervisor New Hampshire - - both temp & perm openings - Histotech South Carolina - Perm - Supervisor and Bench - Histotech - Mon- Fri Thank you, Eric Dye-Sr Allied Healthcare Recruiter 800-466-9919 ext 223 or Cell - 407-756-5507 P.S.: Feel free to pass along this email and my phone number to anyone who you think might be interested. P.S.S.: The clients are currently interviewing - and the job will close soon - so if you are interested, please call me today at 1-800-466-9919 ext 229 --------------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing 800 466 9919 ext 223 - voice 407 671 6075 - fax eric@ategra.com To Learn More About Ategra: http://www.ategra.com ---------------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. ---------------------------------------------------------------- ---------------------------------------------------------------- Note: this message is intended for: Fellow-Histonetters at histonet@lists.utsouthwestern.edu ---------------------------------------------------------------- From PKamalavenkatesh <@t> wockhardtin.com Mon Jun 26 00:36:40 2006 From: PKamalavenkatesh <@t> wockhardtin.com (PKamalavenkatesh@wockhardtin.com) Date: Mon Jun 26 00:29:27 2006 Subject: [Histonet] PAS-H staining-rat seminiferous epithelium-doubts reg Message-ID: Dear Histonetters, This is with regard to PAS-Hotchkiss staining of rat seminiferous tubules for staging of spermatogenesis. This is the protocol I have followed. Testes got fixed in Bouin?s for 24 hrs and washed with 70 % alcohol and stored in the same. This protocol I took from histonet archives (Thanks to Bettina Hutz, Research Assistant, Department of Discovery Biology Orion Corporation, ORION PHARMA) Deparaffination 3x xylene each min. 3 minutes 2x ethanol 100% short ethanol 90% short ethanol 80% short ethanol 70% short Staining Periodic acid (oxidation) 5 minutes 70% ethanol 1 minute Reduction solution 1 minute 70% ethanol 1 minute Schiff???s reagent 20 minutes Running tap water 10 minutes Mayer???s hemalaun 3 minutes Running tap water 10 minutes Dehydration & mounting ethanol 70% short ethanol 90% short 2x ethanol 100% short 3x xylene short Mount with xylene based medium But I am feeling that the staining quality is inferior (staining of the acrosomes) to correctly detect the various stages. I have few pictures published by the European Society of Toxicopathology, which are simply fantastic. I don?t know where I am failing. Now my question is any of our members are having a protocol, which they feel working very well and anybody can able to send me the photos of various stages of the rat spermatogenesis they got. This will enable me to compare my staining with that of others. REGARDS Dr.P.Kamalavenkatesh Pre clinical safety assessment division New Drug Discovery- Biology Wockhardt Research Center India Please Visit our New Corporate Web Site www.wockhardt.com --------------------- Disclaimer ------------------------------------------------------------------------------ Information transmitted by this E-MAIL is proprietary to Wockhardt Ltd. and/ or its Group Companies and/or,its Customers and is intended for use only by the individual or entity to which it is addressed, and may contain information that is privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. --------------------------------------------------------------------------------------------------------------- From kemlo.rogerson <@t> waht.swest.nhs.uk Mon Jun 26 02:14:10 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Jun 26 02:13:41 2006 Subject: [Histonet] Melanin bleach Message-ID: Melanins are a variety of pigments and can be found in skin, brain, nerve root ganglia and may even be classified as lipofuchsin. Some are iron free and others contain between 50 to 60% carbon, 4 to 6.6% hydrogen, 9 to 14% nitrogen and sulphur between 1 to 12%. My point is that the melanins bleach at very different rates with times ranging from 5 min for the Neuromelainins to 4 hrs for Ocular melanins with 0.25% KMNo4 at 25 degrees C. You may know when it's gone that it was melanin but if its still there you won't know that it still could be a resistant one, unless you stain for it afterwards. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Each one sees what he carries in his heart. --Johann Wolfgang von Goethe This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From kemlo.rogerson <@t> waht.swest.nhs.uk Mon Jun 26 02:20:46 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Jun 26 02:20:20 2006 Subject: [Histonet] Has anyone done a comprehensive study on fixatives ? Message-ID: This type of project has been done many, many times by Biomedical scientists studying for their Honours; even did it myself for my HND. I'm sure if you invest in a good General Histology Book you will find it again, and again! Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Each one sees what he carries in his heart. --Johann Wolfgang von Goethe This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Malcolm.McCallum <@t> tamut.edu Mon Jun 26 08:19:29 2006 From: Malcolm.McCallum <@t> tamut.edu (Malcolm McCallum) Date: Mon Jun 26 08:21:46 2006 Subject: [Histonet] why PAS-H staining instead of H-E for testes?? References: Message-ID: Saw the below email. In conducting life history studies I have used H-E to characterize stages of spermatogenesis. IS there an advantage to PAS-H over H-E? I assume you must be able to see something you can't with H-E! Or is this specific for rats? I do amphibians and reptiles! VISIT HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org A New Journal Published in Partnership with Partners in Amphibian and Reptile Conservaiton and the World Congress of Herpetology. Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-223-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of PKamalavenkatesh@wockhardtin.com Sent: Mon 6/26/2006 12:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAS-H staining-rat seminiferous epithelium-doubts reg Dear Histonetters, This is with regard to PAS-Hotchkiss staining of rat seminiferous tubules for staging of spermatogenesis. This is the protocol I have followed. Testes got fixed in Bouin's for 24 hrs and washed with 70 % alcohol and stored in the same. This protocol I took from histonet archives (Thanks to Bettina Hutz, Research Assistant, Department of Discovery Biology Orion Corporation, ORION PHARMA) Deparaffination 3x xylene each min. 3 minutes 2x ethanol 100% short ethanol 90% short ethanol 80% short ethanol 70% short Staining Periodic acid (oxidation) 5 minutes 70% ethanol 1 minute Reduction solution 1 minute 70% ethanol 1 minute Schiff???s reagent 20 minutes Running tap water 10 minutes Mayer???s hemalaun 3 minutes Running tap water 10 minutes Dehydration & mounting ethanol 70% short ethanol 90% short 2x ethanol 100% short 3x xylene short Mount with xylene based medium But I am feeling that the staining quality is inferior (staining of the acrosomes) to correctly detect the various stages. I have few pictures published by the European Society of Toxicopathology, which are simply fantastic. I don't know where I am failing. Now my question is any of our members are having a protocol, which they feel working very well and anybody can able to send me the photos of various stages of the rat spermatogenesis they got. This will enable me to compare my staining with that of others. REGARDS Dr.P.Kamalavenkatesh Pre clinical safety assessment division New Drug Discovery- Biology Wockhardt Research Center India Please Visit our New Corporate Web Site www.wockhardt.com --------------------- Disclaimer ------------------------------------------------------------------------------ Information transmitted by this E-MAIL is proprietary to Wockhardt Ltd. and/ or its Group Companies and/or,its Customers and is intended for use only by the individual or entity to which it is addressed, and may contain information that is privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. --------------------------------------------------------------------------------------------------------------- From JMahoney <@t> alegent.org Mon Jun 26 08:24:18 2006 From: JMahoney <@t> alegent.org (Janice Mahoney) Date: Mon Jun 26 08:24:33 2006 Subject: [Histonet] salary for lab assistants Message-ID: I completely agree. Weighing and measuring is grossing and the person who does so must qualify under CLIA (CAP follows CLIA). Many institutions are still allowing this grossing to be performed HT's and assistants who do not qualify. I have been performing CAP inspections for many years and have cited labs for this. I did my homework and have documentation from CLIA to substantiate the fact that "measuring, counting and weighing" is indeed grossing. Jan M. Omaha >>> "Ron Martin" 06/25/2006 11:21 AM >>> Should this lab assistant be "cassetting" at all? This sounds like a grossing tech to me and should fall under CLIA regs for high complex testing with the appropriate education/training. If this person isn't qualified should he/she be performing these duties at all? Ron Martin -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Sunday, June 25, 2006 9:38 AM To: Pam Mara; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] salary for lab assistants Pam: For your description your assistant works as a histotech although she may or may not have "academic qualifications". You have to consider also that initially histotechs were trained "on the job" and later on were "grandfathered" at licensure time (perhaps this is one of the reasons why historically histotechs are in lower echelon of clinical lab salary scale, but that is another subject to talk about!). I do not think that you should ask about lab aides paymen rates when your aide is performing tasks above those of that job position. As a lab supervisor/manager it should be part of your mission that of assuring that the employees you supervise are treated fairly. Having said all that I think that you should get for her a salary that reflects her tasks, not those usually corresponding for a job level. It is my opinion that she shoudl be paid, at least, at the histotech entry level. Hope this will help you! Ren? J. Pam Mara wrote: I have a question. We have a lab assistant here who does the "cassetting" of tissue specimens. She isn't a PA and she doesn't really gross in any large specimens. We mainly have GI specimens and biopsies, which she weighs/measures and puts into cassettes. She also helps with staining, coverslipping, etc. I was just wondering what is a reasonable salary for someone who performs these tasks? Does anyone else work with a lab assistant that performs similar tasks? Thanks for the info, Pam Mara -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Saturday, June 24, 2006 9:52 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 31, Issue 36 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Want to be your own boss? Learn how on Yahoo! Small Business. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cking <@t> rallansci.com Mon Jun 26 08:41:15 2006 From: cking <@t> rallansci.com (King, Curtis - RAS) Date: Mon Jun 26 08:41:24 2006 Subject: [Histonet] why PAS-H staining instead of H-E for testes?? Message-ID: <490C1EC04BA10F4891494D0ED756AC9303C94EA2@usmi0012k03.rallansci.com> When I did sperm staging I processed Bouins fixed testis to GMA block and cut 1 micron thick sections. I then stained with PASH and had wonderful results. My thoughts are that you will not see the acrosomes well enough to stage them on a 4 to 5 micron thick paraffin section. If I remember correctly the GLP regulations require GMA processing for sperm staging. Hope this helps, Curtis D. King, HT(ASCP) Tech Rite Consultant Richard Allan Scientific 4481 Campus Drive Kalamazoo, MI 49008 Phone: 800-522-7270 ext 672 Fax: 296-372-2734 E-Mail: cking@rallansci.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Malcolm McCallum Sent: Monday, June 26, 2006 9:19 AM To: PKamalavenkatesh@wockhardtin.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] why PAS-H staining instead of H-E for testes?? Saw the below email. In conducting life history studies I have used H-E to characterize stages of spermatogenesis. IS there an advantage to PAS-H over H-E? I assume you must be able to see something you can't with H-E! Or is this specific for rats? I do amphibians and reptiles! VISIT HERPETOLOGICAL CONSERVATION AND BIOLOGY www.herpconbio.org A New Journal Published in Partnership with Partners in Amphibian and Reptile Conservaiton and the World Congress of Herpetology. Malcolm L. McCallum Assistant Professor Department of Biological Sciences Texas A&M University Texarkana 2600 Robison Rd. Texarkana, TX 75501 O: 1-903-223-3134 H: 1-903-791-3843 Homepage: https://www.eagle.tamut.edu/faculty/mmccallum/index.html ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of PKamalavenkatesh@wockhardtin.com Sent: Mon 6/26/2006 12:36 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PAS-H staining-rat seminiferous epithelium-doubts reg Dear Histonetters, This is with regard to PAS-Hotchkiss staining of rat seminiferous tubules for staging of spermatogenesis. This is the protocol I have followed. Testes got fixed in Bouin's for 24 hrs and washed with 70 % alcohol and stored in the same. This protocol I took from histonet archives (Thanks to Bettina Hutz, Research Assistant, Department of Discovery Biology Orion Corporation, ORION PHARMA) Deparaffination 3x xylene each min. 3 minutes 2x ethanol 100% short ethanol 90% short ethanol 80% short ethanol 70% short Staining Periodic acid (oxidation) 5 minutes 70% ethanol 1 minute Reduction solution 1 minute 70% ethanol 1 minute Schiff???s reagent 20 minutes Running tap water 10 minutes Mayer???s hemalaun 3 minutes Running tap water 10 minutes Dehydration & mounting ethanol 70% short ethanol 90% short 2x ethanol 100% short 3x xylene short Mount with xylene based medium But I am feeling that the staining quality is inferior (staining of the acrosomes) to correctly detect the various stages. I have few pictures published by the European Society of Toxicopathology, which are simply fantastic. I don't know where I am failing. Now my question is any of our members are having a protocol, which they feel working very well and anybody can able to send me the photos of various stages of the rat spermatogenesis they got. This will enable me to compare my staining with that of others. REGARDS Dr.P.Kamalavenkatesh Pre clinical safety assessment division New Drug Discovery- Biology Wockhardt Research Center India Please Visit our New Corporate Web Site www.wockhardt.com --------------------- Disclaimer ---------------------------------------------------------------------------- -- Information transmitted by this E-MAIL is proprietary to Wockhardt Ltd. and/ or its Group Companies and/or,its Customers and is intended for use only by the individual or entity to which it is addressed, and may contain information that is privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. ---------------------------------------------------------------------------- ----------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Mon Jun 26 08:45:52 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Mon Jun 26 08:45:15 2006 Subject: [Histonet] PAS-H staining-rat seminiferous epithelium-doubts reg In-Reply-To: References: Message-ID: <449FE510.5080803@umdnj.edu> Here are my suggestions for your PAS staining. 1. Make periodic acid fresh each day. 2. What is the "reduction solution" you are using after the periodic acid? Why oxidize and then reduce?? Delete this step. 3. Follow the Schiff's reagent with 3 changes of fresh 0.5% sodium metabisulfite, 1-2 minutes each. NOT running water. This will make the stain more precise. 4. Your dehydration and clearing steps may be too short to remove all water and alcohol, there is no reason for one to hurry these steps. 5. Be sure your Schiff's reagent is good, test by putting one drop into formaldehyde, it should turn magenta/pink within a few seconds. If not make fresh. Geoff PKamalavenkatesh@wockhardtin.com wrote: >Dear Histonetters, > >This is with regard to PAS-Hotchkiss staining of rat seminiferous tubules > >for staging of spermatogenesis. This is the protocol I have followed. > >Testes got fixed in Bouin?s for 24 hrs and washed with 70 % alcohol and > >stored in the same. This protocol I took from histonet archives (Thanks to > >Bettina Hutz, Research Assistant, Department of Discovery Biology Orion > >Corporation, ORION PHARMA) > > >Deparaffination > >3x xylene each min. 3 minutes >2x ethanol 100% short >ethanol 90% short >ethanol 80% short >ethanol 70% short > >Staining >Periodic acid (oxidation) 5 minutes >70% ethanol 1 minute >Reduction solution 1 minute >70% ethanol 1 minute >Schiff???s reagent 20 minutes >Running tap water 10 minutes >Mayer???s hemalaun 3 minutes >Running tap water 10 minutes > >Dehydration & mounting >ethanol 70% short >ethanol 90% short >2x ethanol 100% short >3x xylene short >Mount with xylene based medium > > > But I am feeling that the staining quality is inferior (staining of the >acrosomes) to correctly detect the various stages. I have few pictures >published by the European Society of Toxicopathology, which are simply >fantastic. I don?t know where I am failing. Now my question is any of our >members are having a protocol, which they feel working very well and >anybody can able to send me the photos of various stages of the rat >spermatogenesis they got. This will enable me to compare my staining with >that of others. > >REGARDS >Dr.P.Kamalavenkatesh >Pre clinical safety assessment division >New Drug Discovery- Biology >Wockhardt Research Center >India >Please Visit our New Corporate Web Site www.wockhardt.com >--------------------- Disclaimer ------------------------------------------------------------------------------ >Information transmitted by this E-MAIL is proprietary to Wockhardt Ltd. and/ or its Group Companies >and/or,its Customers and is intended for use only by the individual or entity to which it is addressed, >and may contain information that is privileged, confidential or exempt from disclosure under applicable law. >If you are not the intended recipient or it appears that this mail has been forwarded to you without proper >authority, you are notified that any use or dissemination of this information in any manner is strictly >prohibited. In such cases, please delete this mail from your records. >--------------------------------------------------------------------------------------------------------------- > > >------------------------------------------------------------------------ > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From Charles.Embrey <@t> carle.com Mon Jun 26 09:33:06 2006 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Mon Jun 26 09:33:11 2006 Subject: [Histonet] salary for lab assistants Message-ID: First off, CAP doesn't exactly follow CLIA. CLIA is more stringent and what you describe is definitely considered grossing and "high complexity testing". CAP, on the other hand, is drawing a distinction between grossing (anything that must be cut in) and non-grossing (taking measurements and putting biopsies in a cassette with no cutting involved). Technically you can meet CAP standards while being in violation of CLIA regulations. If the lab does any billing with the government, (Medicare, Medicaid, Tricare) then you had better be meeting CLIA '88. Personally I thing CAP is so wrong for clouding the water on this one. Charles Embrey, PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Monday, June 26, 2006 8:24 AM To: Pathrm35@adelphia.net; pamm@hlclab.com; histonet@lists.utsouthwestern.edu; rjbuesa@yahoo.com Subject: RE: [Histonet] salary for lab assistants I completely agree. Weighing and measuring is grossing and the person who does so must qualify under CLIA (CAP follows CLIA). Many institutions are still allowing this grossing to be performed HT's and assistants who do not qualify. I have been performing CAP inspections for many years and have cited labs for this. I did my homework and have documentation from CLIA to substantiate the fact that "measuring, counting and weighing" is indeed grossing. Jan M. Omaha >>> "Ron Martin" 06/25/2006 11:21 AM >>> Should this lab assistant be "cassetting" at all? This sounds like a grossing tech to me and should fall under CLIA regs for high complex testing with the appropriate education/training. If this person isn't qualified should he/she be performing these duties at all? Ron Martin -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Sunday, June 25, 2006 9:38 AM To: Pam Mara; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] salary for lab assistants Pam: For your description your assistant works as a histotech although she may or may not have "academic qualifications". You have to consider also that initially histotechs were trained "on the job" and later on were "grandfathered" at licensure time (perhaps this is one of the reasons why historically histotechs are in lower echelon of clinical lab salary scale, but that is another subject to talk about!). I do not think that you should ask about lab aides paymen rates when your aide is performing tasks above those of that job position. As a lab supervisor/manager it should be part of your mission that of assuring that the employees you supervise are treated fairly. Having said all that I think that you should get for her a salary that reflects her tasks, not those usually corresponding for a job level. It is my opinion that she shoudl be paid, at least, at the histotech entry level. Hope this will help you! Ren? J. Pam Mara wrote: I have a question. We have a lab assistant here who does the "cassetting" of tissue specimens. She isn't a PA and she doesn't really gross in any large specimens. We mainly have GI specimens and biopsies, which she weighs/measures and puts into cassettes. She also helps with staining, coverslipping, etc. I was just wondering what is a reasonable salary for someone who performs these tasks? Does anyone else work with a lab assistant that performs similar tasks? Thanks for the info, Pam Mara -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Saturday, June 24, 2006 9:52 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 31, Issue 36 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Want to be your own boss? Learn how on Yahoo! Small Business. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Mon Jun 26 11:13:54 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Jun 26 11:13:59 2006 Subject: [Histonet] Fixative studies In-Reply-To: References: Message-ID: <6.0.0.22.1.20060626100220.01b78d60@gemini.msu.montana.edu> Thanks to Barry for this wonderful list. One reference, #4 Beckstead is actually about a formalin-free fixative, Zinc Tris Buffer with paraffin embedded tissues when CD markers are compromised by formalin or PFA fixation. Beckstead did this study for human CD markers in lymphomas. At 02:03 PM 6/23/2006, you wrote: >Some suggestions. >This is a short list. >Barry > >1. Mann, Gustav 1902. Physiological Histology. Methods and Theory. >Oxford at the Clarendon Press. This text deals in detail with methods in >histology and for fixation with the history and rationale behind using >various chemical agents as fixatives. > >2. Pearse A.G.E. The Chemistry and practice of Fixation. Chapter 5 >pages 97 -158. In Histochemistry Theoretical and Applied vol 1. 4th >edition. Preparative and Optical Techniques. 1980. Excellent description >of fixation chemistry and extensive list of references. vol. 2. >Analytical techniques 1985. vol 3. Enzyme Histochemistry 1991. Churchill >Livingstone, London. > >3. Arnold M.M., Srivastava S., Fredenburgh J., Stockyard C.R. and >Myers R.B. 1996. Effects of fixation and tissue processing on >immunohistochemical demonstration of specific antigens. Biotechnology & >Histochemistry 71: 224-230. > >4. Beckstead J.H. 1994. A Simple Technique for Preservation of >Fixative-sensitive Antigens in Paraffin-embedded Tissues: J. Histochem. >Cytochem. 43: 345. > >5. Isaam Eltoum, Jerry Fredenburgh, Russel B. Myers and William >Grizzle. 2001. Introduction to the theory and Practice of Fixation of >Tissues. J. Histotechnology 24 #3 173- 190. > >6. Hopwood D. 1985. Cell and tissue fixation, 1972 - 1982. >Histochemical Journal. 17: 389-442 > >7. Prento P and Lyon H. 1997. Commercial Formalin Substitutes for >Histopathology. Biotechnic & Histochemistry. 72 (5). 272-282. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From Tbarnhart <@t> primecare.org Mon Jun 26 11:44:22 2006 From: Tbarnhart <@t> primecare.org (Barnhart, Tammy) Date: Mon Jun 26 11:44:36 2006 Subject: [Histonet] salary for lab assistants Message-ID: <1779904B5E82D511914C00D0B79333920E48E908@exchangent> After reviewing the CAP checklist as it relates to grossing of specimens (see below), I don't think I would assume that the CAP is not referring to what CLIA considers "High Complexity Testing". CLIA is very clear about educational and training requirements needed to perform high complexity testing. Granted the CAP has caused a lot of confusion by using the term "dissected by individuals other than pathologist" instead of the term "tissue examination" as CLIA has used, but that may be viewed as splitting hairs. I have assumed that the CLIA and CAP requirements are the same and like Janice, have cited labs I have inspected for not having qualified personnel performing this function. Charles, do you have a source for the government requirements for tissue examination and billing? I would really like to have that documentation for my files. I have asked the CAP to respond to this question via e-mail and will share their response with the list as soon as it is received. Tammy Barnhart, BS, HTL(ASCP) Anatomic Pathology Supervisor St. Alexius Medical Center Bismarck, ND **NEW** 04/28/2005 ANP.11610 Phase II N/A YES NO If specimens are dissected by individuals other than a pathologist or pathology resident, do such individuals qualify as high complexity testing personnel under CLIA-88 regulations? NOTE: The laboratory director may delegate the dissection of specimens to non-pathologist individuals; these individuals must be qualified as high complexity testing personnel under CLIA-88 regulations. The minimum training/experience required of such personnel is: 1. An earned associate degree in a laboratory science or medical laboratory technology, obtained from an accredited institution, OR 2. Education/training equivalent to the above that includes at least 60 semester hours or equivalent from an accredited institution. This education must include 24 semester hours of medical laboratory technology courses, OR 24 semester hours of science courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination. In addition, the individual must have laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, the CAHEA, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above), OR at least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. In addition, the CLIA-88 regulations include exceptions for grandfathered individuals; these regulations (42CFR493.1489 and 1491) may be found at http://www.phppo.cdc.gov/clia/regs/subpart_m.aspx#493.1487 This checklist question applies only to laboratories subject to CLIA-88. COMMENTARY: N/A REFERENCE: Department of Health and Human Services, Centers for Medicare and Medicaid Services. Clinical laboratory improvement amendments of 1988; final rule. Fed Register. 2003(Oct 1):1070-1071 [42CFR493.1489], 1071-1072 [42CFR493.1491]. ANP.11620 Phase II N/A YES NO Are the types of specimens examined and the extent of the examination performed by a non pathologist clearly defined in a documented policy or protocol? COMMENTARY: N/A REFERENCES: 1) Department of Health and Human Services, Centers for Medicare and Medicaid Services. Clinical laboratory improvement amendments of 1988; final rule. Fed Register. 1992(Feb 28):7183 [42CFR493.1489(b)(6)]; 2) Cibull ML. Q&A. Northfield, IL: College of American Pathologists CAP Today. 1997;11(7):112; 3) Grzybicki DM, et al. National practice characteristics and utilization of pathologists' assistants. Arch Pathol Lab Med. 2001;125:905-912. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Charles.Embrey Sent: Monday, June 26, 2006 9:33 AM To: Janice Mahoney; Pathrm35@adelphia.net; pamm@hlclab.com; histonet@lists.utsouthwestern.edu; rjbuesa@yahoo.com Subject: RE: [Histonet] salary for lab assistants First off, CAP doesn't exactly follow CLIA. CLIA is more stringent and what you describe is definitely considered grossing and "high complexity testing". CAP, on the other hand, is drawing a distinction between grossing (anything that must be cut in) and non-grossing (taking measurements and putting biopsies in a cassette with no cutting involved). Technically you can meet CAP standards while being in violation of CLIA regulations. If the lab does any billing with the government, (Medicare, Medicaid, Tricare) then you had better be meeting CLIA '88. Personally I thing CAP is so wrong for clouding the water on this one. Charles Embrey, PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Monday, June 26, 2006 8:24 AM To: Pathrm35@adelphia.net; pamm@hlclab.com; histonet@lists.utsouthwestern.edu; rjbuesa@yahoo.com Subject: RE: [Histonet] salary for lab assistants I completely agree. Weighing and measuring is grossing and the person who does so must qualify under CLIA (CAP follows CLIA). Many institutions are still allowing this grossing to be performed HT's and assistants who do not qualify. I have been performing CAP inspections for many years and have cited labs for this. I did my homework and have documentation from CLIA to substantiate the fact that "measuring, counting and weighing" is indeed grossing. Jan M. Omaha >>> "Ron Martin" 06/25/2006 11:21 AM >>> Should this lab assistant be "cassetting" at all? This sounds like a grossing tech to me and should fall under CLIA regs for high complex testing with the appropriate education/training. If this person isn't qualified should he/she be performing these duties at all? Ron Martin -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Sunday, June 25, 2006 9:38 AM To: Pam Mara; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] salary for lab assistants Pam: For your description your assistant works as a histotech although she may or may not have "academic qualifications". You have to consider also that initially histotechs were trained "on the job" and later on were "grandfathered" at licensure time (perhaps this is one of the reasons why historically histotechs are in lower echelon of clinical lab salary scale, but that is another subject to talk about!). I do not think that you should ask about lab aides paymen rates when your aide is performing tasks above those of that job position. As a lab supervisor/manager it should be part of your mission that of assuring that the employees you supervise are treated fairly. Having said all that I think that you should get for her a salary that reflects her tasks, not those usually corresponding for a job level. It is my opinion that she shoudl be paid, at least, at the histotech entry level. Hope this will help you! Ren? J. Pam Mara wrote: I have a question. We have a lab assistant here who does the "cassetting" of tissue specimens. She isn't a PA and she doesn't really gross in any large specimens. We mainly have GI specimens and biopsies, which she weighs/measures and puts into cassettes. She also helps with staining, coverslipping, etc. I was just wondering what is a reasonable salary for someone who performs these tasks? Does anyone else work with a lab assistant that performs similar tasks? Thanks for the info, Pam Mara -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Saturday, June 24, 2006 9:52 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 31, Issue 36 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Want to be your own boss? Learn how on Yahoo! Small Business. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice:This e-mail message is for sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, distribution, or copying is prohibited. If you are not the intended recipient, please contact the sender by replying to this e-mail and destroy/delete all copies of this e-mail message. From Charles.Embrey <@t> carle.com Mon Jun 26 12:16:42 2006 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Mon Jun 26 12:16:53 2006 Subject: [Histonet] salary for lab assistants Message-ID: Sorry to say that your assumption is wrong. I have already e-mail CAP and here is my e-mail and their response. They make it very clear that they are deviating from CLIA. Personally I think the whole thing stinks and CAP is wrong for what they have done here. Dear Mr. Embry: I am responding to your inquiry regarding the dissection of patient specimen material by someone other than a pathologist. The intent is that the requirements of ANP.11610 apply to personnel who evaluate and dissect specimens, and select which samples of tissue are to be submitted for histologic processing. The question is not intended to apply to persons who merely transfer a small specimen from a bottle to a cassette. This latter process does not require the judgment of the former. There may be "gray" areas in this distinction, and the laboratory director is responsible for dealing with those circumstances. I am also copying your e-mail to Stephen J. Sarewitz, MD, CAP checklist commissioner, and Francis E. Sharkey, MD, CAP education commissioner for informational purposes and possible future checklist revision. Thank you for your inquiry of the Laboratory Accreditation Program. -----Original Message----- From: Charles.Embrey [mailto:Charles.Embrey@carle.com] Sent: Tuesday, May 02, 2006 12:42 PM To: CommEmail Subject: Question concerning non-pathologists grossing ANP.11600 refers to "specimens examined grossly" but the next question, ANP.11610 has changed the phrase "examined grossly" to "are dissected". Is CAP creating a "loophole" for labs to use Non-high complexity testing personnel to do biopsies and small specimens? The federal register CLIA interpretive guidelines appendix C subpart M effective April 24, 2003 states in section 493.1461(e) "In the case if gross examinations, the technical supervisor may delegate to individuals qualified under 493.1498 the responsibility for the physical examination/description, including color, weight, measurement, and other characteristics of the tissue; or other mechanical procedures for which a specific written protocol has been developed." Is CAP going to accept non-high complexity testing personnel to gross? Why the two different wordings? Charles Embrey PA(ASCP) Manager Histology Carle Clinic IL (217) 383-3666 -----Original Message----- From: Barnhart, Tammy [mailto:Tbarnhart@primecare.org] Sent: Monday, June 26, 2006 11:44 AM To: Charles.Embrey; Janice Mahoney; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] salary for lab assistants After reviewing the CAP checklist as it relates to grossing of specimens (see below), I don't think I would assume that the CAP is not referring to what CLIA considers "High Complexity Testing". CLIA is very clear about educational and training requirements needed to perform high complexity testing. Granted the CAP has caused a lot of confusion by using the term "dissected by individuals other than pathologist" instead of the term "tissue examination" as CLIA has used, but that may be viewed as splitting hairs. I have assumed that the CLIA and CAP requirements are the same and like Janice, have cited labs I have inspected for not having qualified personnel performing this function. Charles, do you have a source for the government requirements for tissue examination and billing? I would really like to have that documentation for my files. I have asked the CAP to respond to this question via e-mail and will share their response with the list as soon as it is received. Tammy Barnhart, BS, HTL(ASCP) Anatomic Pathology Supervisor St. Alexius Medical Center Bismarck, ND **NEW** 04/28/2005 ANP.11610 Phase II N/A YES NO If specimens are dissected by individuals other than a pathologist or pathology resident, do such individuals qualify as high complexity testing personnel under CLIA-88 regulations? NOTE: The laboratory director may delegate the dissection of specimens to non-pathologist individuals; these individuals must be qualified as high complexity testing personnel under CLIA-88 regulations. The minimum training/experience required of such personnel is: 1. An earned associate degree in a laboratory science or medical laboratory technology, obtained from an accredited institution, OR 2. Education/training equivalent to the above that includes at least 60 semester hours or equivalent from an accredited institution. This education must include 24 semester hours of medical laboratory technology courses, OR 24 semester hours of science courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination. In addition, the individual must have laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, the CAHEA, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above), OR at least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. In addition, the CLIA-88 regulations include exceptions for grandfathered individuals; these regulations (42CFR493.1489 and 1491) may be found at http://www.phppo.cdc.gov/clia/regs/subpart_m.aspx#493.1487 This checklist question applies only to laboratories subject to CLIA-88. COMMENTARY: N/A REFERENCE: Department of Health and Human Services, Centers for Medicare and Medicaid Services. Clinical laboratory improvement amendments of 1988; final rule. Fed Register. 2003(Oct 1):1070-1071 [42CFR493.1489], 1071-1072 [42CFR493.1491]. ANP.11620 Phase II N/A YES NO Are the types of specimens examined and the extent of the examination performed by a non pathologist clearly defined in a documented policy or protocol? COMMENTARY: N/A REFERENCES: 1) Department of Health and Human Services, Centers for Medicare and Medicaid Services. Clinical laboratory improvement amendments of 1988; final rule. Fed Register. 1992(Feb 28):7183 [42CFR493.1489(b)(6)]; 2) Cibull ML. Q&A. Northfield, IL: College of American Pathologists CAP Today. 1997;11(7):112; 3) Grzybicki DM, et al. National practice characteristics and utilization of pathologists' assistants. Arch Pathol Lab Med. 2001;125:905-912. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Charles.Embrey Sent: Monday, June 26, 2006 9:33 AM To: Janice Mahoney; Pathrm35@adelphia.net; pamm@hlclab.com; histonet@lists.utsouthwestern.edu; rjbuesa@yahoo.com Subject: RE: [Histonet] salary for lab assistants First off, CAP doesn't exactly follow CLIA. CLIA is more stringent and what you describe is definitely considered grossing and "high complexity testing". CAP, on the other hand, is drawing a distinction between grossing (anything that must be cut in) and non-grossing (taking measurements and putting biopsies in a cassette with no cutting involved). Technically you can meet CAP standards while being in violation of CLIA regulations. If the lab does any billing with the government, (Medicare, Medicaid, Tricare) then you had better be meeting CLIA '88. Personally I thing CAP is so wrong for clouding the water on this one. Charles Embrey, PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Monday, June 26, 2006 8:24 AM To: Pathrm35@adelphia.net; pamm@hlclab.com; histonet@lists.utsouthwestern.edu; rjbuesa@yahoo.com Subject: RE: [Histonet] salary for lab assistants I completely agree. Weighing and measuring is grossing and the person who does so must qualify under CLIA (CAP follows CLIA). Many institutions are still allowing this grossing to be performed HT's and assistants who do not qualify. I have been performing CAP inspections for many years and have cited labs for this. I did my homework and have documentation from CLIA to substantiate the fact that "measuring, counting and weighing" is indeed grossing. Jan M. Omaha >>> "Ron Martin" 06/25/2006 11:21 AM >>> Should this lab assistant be "cassetting" at all? This sounds like a grossing tech to me and should fall under CLIA regs for high complex testing with the appropriate education/training. If this person isn't qualified should he/she be performing these duties at all? Ron Martin -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Sunday, June 25, 2006 9:38 AM To: Pam Mara; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] salary for lab assistants Pam: For your description your assistant works as a histotech although she may or may not have "academic qualifications". You have to consider also that initially histotechs were trained "on the job" and later on were "grandfathered" at licensure time (perhaps this is one of the reasons why historically histotechs are in lower echelon of clinical lab salary scale, but that is another subject to talk about!). I do not think that you should ask about lab aides paymen rates when your aide is performing tasks above those of that job position. As a lab supervisor/manager it should be part of your mission that of assuring that the employees you supervise are treated fairly. Having said all that I think that you should get for her a salary that reflects her tasks, not those usually corresponding for a job level. It is my opinion that she shoudl be paid, at least, at the histotech entry level. Hope this will help you! Ren? J. Pam Mara wrote: I have a question. We have a lab assistant here who does the "cassetting" of tissue specimens. She isn't a PA and she doesn't really gross in any large specimens. We mainly have GI specimens and biopsies, which she weighs/measures and puts into cassettes. She also helps with staining, coverslipping, etc. I was just wondering what is a reasonable salary for someone who performs these tasks? Does anyone else work with a lab assistant that performs similar tasks? Thanks for the info, Pam Mara -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Saturday, June 24, 2006 9:52 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 31, Issue 36 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Want to be your own boss? Learn how on Yahoo! Small Business. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice:This e-mail message is for sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, distribution, or copying is prohibited. If you are not the intended recipient, please contact the sender by replying to this e-mail and destroy/delete all copies of this e-mail message. From Tbarnhart <@t> primecare.org Mon Jun 26 12:28:28 2006 From: Tbarnhart <@t> primecare.org (Barnhart, Tammy) Date: Mon Jun 26 12:28:44 2006 Subject: [Histonet] salary for lab assistants Message-ID: <1779904B5E82D511914C00D0B79333920E48E909@exchangent> Well, I have to agree that this stinks. Either gross tissue examination is high complexity testing or it is not. This is just creating confusion. How are you to know when you inspect a lab whether the person doing gross is always just transferring the specimen and never bisecting that larger polyp or cervical bx? Or does this mean as long as the entire specimen is submitted, then it OK not to have the qualifications? They have, once again, managed to confuse just about everyone and in the process make the inspection process that much more arbitrary. Charles, do you have the source for government billing regulations? Tammy Barnhart, BS, HTL(ASCP) Anatomic Pathology Supervisor St. Alexius Medical Center Bismarck, ND -----Original Message----- From: Charles.Embrey [mailto:Charles.Embrey@carle.com] Sent: Monday, June 26, 2006 12:17 PM To: Barnhart, Tammy Cc: Histonet@pathology.swmed.edu Subject: RE: [Histonet] salary for lab assistants Sorry to say that your assumption is wrong. I have already e-mail CAP and here is my e-mail and their response. They make it very clear that they are deviating from CLIA. Personally I think the whole thing stinks and CAP is wrong for what they have done here. Dear Mr. Embry: I am responding to your inquiry regarding the dissection of patient specimen material by someone other than a pathologist. The intent is that the requirements of ANP.11610 apply to personnel who evaluate and dissect specimens, and select which samples of tissue are to be submitted for histologic processing. The question is not intended to apply to persons who merely transfer a small specimen from a bottle to a cassette. This latter process does not require the judgment of the former. There may be "gray" areas in this distinction, and the laboratory director is responsible for dealing with those circumstances. I am also copying your e-mail to Stephen J. Sarewitz, MD, CAP checklist commissioner, and Francis E. Sharkey, MD, CAP education commissioner for informational purposes and possible future checklist revision. Thank you for your inquiry of the Laboratory Accreditation Program. -----Original Message----- From: Charles.Embrey [mailto:Charles.Embrey@carle.com] Sent: Tuesday, May 02, 2006 12:42 PM To: CommEmail Subject: Question concerning non-pathologists grossing ANP.11600 refers to "specimens examined grossly" but the next question, ANP.11610 has changed the phrase "examined grossly" to "are dissected". Is CAP creating a "loophole" for labs to use Non-high complexity testing personnel to do biopsies and small specimens? The federal register CLIA interpretive guidelines appendix C subpart M effective April 24, 2003 states in section 493.1461(e) "In the case if gross examinations, the technical supervisor may delegate to individuals qualified under 493.1498 the responsibility for the physical examination/description, including color, weight, measurement, and other characteristics of the tissue; or other mechanical procedures for which a specific written protocol has been developed." Is CAP going to accept non-high complexity testing personnel to gross? Why the two different wordings? Charles Embrey PA(ASCP) Manager Histology Carle Clinic IL (217) 383-3666 -----Original Message----- From: Barnhart, Tammy [mailto:Tbarnhart@primecare.org] Sent: Monday, June 26, 2006 11:44 AM To: Charles.Embrey; Janice Mahoney; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] salary for lab assistants After reviewing the CAP checklist as it relates to grossing of specimens (see below), I don't think I would assume that the CAP is not referring to what CLIA considers "High Complexity Testing". CLIA is very clear about educational and training requirements needed to perform high complexity testing. Granted the CAP has caused a lot of confusion by using the term "dissected by individuals other than pathologist" instead of the term "tissue examination" as CLIA has used, but that may be viewed as splitting hairs. I have assumed that the CLIA and CAP requirements are the same and like Janice, have cited labs I have inspected for not having qualified personnel performing this function. Charles, do you have a source for the government requirements for tissue examination and billing? I would really like to have that documentation for my files. I have asked the CAP to respond to this question via e-mail and will share their response with the list as soon as it is received. Tammy Barnhart, BS, HTL(ASCP) Anatomic Pathology Supervisor St. Alexius Medical Center Bismarck, ND **NEW** 04/28/2005 ANP.11610 Phase II N/A YES NO If specimens are dissected by individuals other than a pathologist or pathology resident, do such individuals qualify as high complexity testing personnel under CLIA-88 regulations? NOTE: The laboratory director may delegate the dissection of specimens to non-pathologist individuals; these individuals must be qualified as high complexity testing personnel under CLIA-88 regulations. The minimum training/experience required of such personnel is: 1. An earned associate degree in a laboratory science or medical laboratory technology, obtained from an accredited institution, OR 2. Education/training equivalent to the above that includes at least 60 semester hours or equivalent from an accredited institution. This education must include 24 semester hours of medical laboratory technology courses, OR 24 semester hours of science courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination. In addition, the individual must have laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, the CAHEA, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above), OR at least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. In addition, the CLIA-88 regulations include exceptions for grandfathered individuals; these regulations (42CFR493.1489 and 1491) may be found at http://www.phppo.cdc.gov/clia/regs/subpart_m.aspx#493.1487 This checklist question applies only to laboratories subject to CLIA-88. COMMENTARY: N/A REFERENCE: Department of Health and Human Services, Centers for Medicare and Medicaid Services. Clinical laboratory improvement amendments of 1988; final rule. Fed Register. 2003(Oct 1):1070-1071 [42CFR493.1489], 1071-1072 [42CFR493.1491]. ANP.11620 Phase II N/A YES NO Are the types of specimens examined and the extent of the examination performed by a non pathologist clearly defined in a documented policy or protocol? COMMENTARY: N/A REFERENCES: 1) Department of Health and Human Services, Centers for Medicare and Medicaid Services. Clinical laboratory improvement amendments of 1988; final rule. Fed Register. 1992(Feb 28):7183 [42CFR493.1489(b)(6)]; 2) Cibull ML. Q&A. Northfield, IL: College of American Pathologists CAP Today. 1997;11(7):112; 3) Grzybicki DM, et al. National practice characteristics and utilization of pathologists' assistants. Arch Pathol Lab Med. 2001;125:905-912. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu ]On Behalf Of Charles.Embrey Sent: Monday, June 26, 2006 9:33 AM To: Janice Mahoney; Pathrm35@adelphia.net; pamm@hlclab.com; histonet@lists.utsouthwestern.edu; rjbuesa@yahoo.com Subject: RE: [Histonet] salary for lab assistants First off, CAP doesn't exactly follow CLIA. CLIA is more stringent and what you describe is definitely considered grossing and "high complexity testing". CAP, on the other hand, is drawing a distinction between grossing (anything that must be cut in) and non-grossing (taking measurements and putting biopsies in a cassette with no cutting involved). Technically you can meet CAP standards while being in violation of CLIA regulations. If the lab does any billing with the government, (Medicare, Medicaid, Tricare) then you had better be meeting CLIA '88. Personally I thing CAP is so wrong for clouding the water on this one. Charles Embrey, PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu ] On Behalf Of Janice Mahoney Sent: Monday, June 26, 2006 8:24 AM To: Pathrm35@adelphia.net; pamm@hlclab.com; histonet@lists.utsouthwestern.edu; rjbuesa@yahoo.com Subject: RE: [Histonet] salary for lab assistants I completely agree. Weighing and measuring is grossing and the person who does so must qualify under CLIA (CAP follows CLIA). Many institutions are still allowing this grossing to be performed HT's and assistants who do not qualify. I have been performing CAP inspections for many years and have cited labs for this. I did my homework and have documentation from CLIA to substantiate the fact that "measuring, counting and weighing" is indeed grossing. Jan M. Omaha >>> "Ron Martin" 06/25/2006 11:21 AM >>> Should this lab assistant be "cassetting" at all? This sounds like a grossing tech to me and should fall under CLIA regs for high complex testing with the appropriate education/training. If this person isn't qualified should he/she be performing these duties at all? Ron Martin -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu ] On Behalf Of Rene J Buesa Sent: Sunday, June 25, 2006 9:38 AM To: Pam Mara; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] salary for lab assistants Pam: For your description your assistant works as a histotech although she may or may not have "academic qualifications". You have to consider also that initially histotechs were trained "on the job" and later on were "grandfathered" at licensure time (perhaps this is one of the reasons why historically histotechs are in lower echelon of clinical lab salary scale, but that is another subject to talk about!). I do not think that you should ask about lab aides paymen rates when your aide is performing tasks above those of that job position. As a lab supervisor/manager it should be part of your mission that of assuring that the employees you supervise are treated fairly. Having said all that I think that you should get for her a salary that reflects her tasks, not those usually corresponding for a job level. It is my opinion that she shoudl be paid, at least, at the histotech entry level. Hope this will help you! Ren? J. Pam Mara wrote: I have a question. We have a lab assistant here who does the "cassetting" of tissue specimens. She isn't a PA and she doesn't really gross in any large specimens. We mainly have GI specimens and biopsies, which she weighs/measures and puts into cassettes. She also helps with staining, coverslipping, etc. I was just wondering what is a reasonable salary for someone who performs these tasks? Does anyone else work with a lab assistant that performs similar tasks? Thanks for the info, Pam Mara -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [ mailto:histonet-bounces@lists.utsouthwestern.edu ] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Saturday, June 24, 2006 9:52 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 31, Issue 36 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Want to be your own boss? Learn how on Yahoo! Small Business. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice:This e-mail message is for sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, distribution, or copying is prohibited. If you are not the intended recipient, please contact the sender by replying to this e-mail and destroy/delete all copies of this e-mail message. Confidentiality Notice:This e-mail message is for sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, distribution, or copying is prohibited. If you are not the intended recipient, please contact the sender by replying to this e-mail and destroy/delete all copies of this e-mail message. From rachael_emerson <@t> urmc.rochester.edu Mon Jun 26 14:06:12 2006 From: rachael_emerson <@t> urmc.rochester.edu (Rachael Emerson) Date: Mon Jun 26 14:06:41 2006 Subject: [Histonet] Chromogens that survive HIER Message-ID: Hello. I am working on a double stain, in which my first round of developing I use Vector Blue. I then do a HIER and proceed.... The Vector Blue stains beautifully and it remains the same even after going through heat induced antigen retrieval. Can anyone tell me if there are other visualizations systems, besides Vector Blue, that can withstand going through HIER? Thank you Rachael Emerson -- Rachael L. Emerson Center for Pediatric Biomedical Research University of Rochester Medical Center 575 Elmwood Avenue MRBX 1.11301 Rochester, NY 14642 From PMonfils <@t> Lifespan.org Mon Jun 26 15:24:50 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Mon Jun 26 17:45:04 2006 Subject: [Histonet] tissue separating from OCT Message-ID: <09C945920A6B654199F7A58A1D7D1FDE01717746@lsexch.lsmaster.lifespan.org> I don't know what causes this sporadic problem, but I have experienced the frustration of it a number of times, primarily with brain specimens that have been frozen in OCT by someone else (I work in a research service lab and you never know what someone will bring in the door). For brains that I freeze myself, I have generally been able to avoid the problem by soaking the cryoprotected specimen in liquid OCT for 15 minutes before freezing it (I use scintillation vials for this purpose). Once a brain is frozen in OCT though, I have found no way of getting around the problem when it is present, other than to thaw it, soak it in OCT, and refreeze it. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > dgaupp@tulane.edu > Sent: Friday, June 23, 2006 4:19 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] tissue separating from OCT > > Hello Histonetters: > > > I am having difficulty sectioning this mouse brain. The tissue is > separating > from OCT upon sectioning. Let me give you a brief background of how the > brain > was processed. Perfused with 4%PFA, fixed overnight at 4C(same fix), next > day > rinsed with 1XPBS, then serially cryoproctected overnight with 20%, 30%, > 40% > sucrose/1XPBS - tissue sunk every time. Rinsed tissue from the sucrose > with > 1XPBS, blot dry, then flash froze in OCT, immersed in isopentane @ -80C. I > am > trying to section at 10um. I've done this procedure before, many, many > times, > never had problem trying to get a nice section. I am about to pull my > hair > out!!! What's the point in putting a tissue that has wrinkles on a slide. > I > don't know what my problem is! I've adjusted the angle on the cryostat > & > adjusted the temperature, changed blades, changed the position of the > tissue to > make almost a full circle(cryostat specimen holder). Can someone in > histoworld > help with this frustration? I thought I was just having a bad cutting > day, but > this is now carried on for over a week. I need to section the entire > brain - > ever piece is precious to me. > > Helpless, > > Dina > > Dina D. Gaupp, B.S., M.T. > Medical Research Specialist > Center for Gene Therapy > Tulane University Health Sciences Center > JBJ Bldg/Rm 658, SL-99 > 1430 Tulane Avenue > New Orleans, LA 70112 > Lab: 504.988.1194 > Fax: 504.988.7710 > Email: dgaupp@tulane.edu > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Amanda.Garcia <@t> TriadHospitals.com Mon Jun 26 14:58:47 2006 From: Amanda.Garcia <@t> TriadHospitals.com (Garcia, Amanda) Date: Mon Jun 26 18:02:51 2006 Subject: [Histonet] (no subject) Message-ID: <8B63039C9DF4554C8FDBF31235F0E1480193E75B@CPRTEVS02.triadhospitals.net> This question is related somewhat to the question of allowing a lab assistant to "cassette" tissue specimens. Where do qualification issues fall regarding weighing and measuring placentas and rolling the fetal membranes and cutting a piece and placing in a cassette? Is this ok for HT's to perform? > Amanda (Amy) Garcia > Histology/Pathology > College Station Medical Center > (979) 680-5372 office > (979) 696-5446 fax > *Mailto:amanda.garcia@triadhospitals.com > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the e-mail or any > of its attachments, please be advised that you have received this > e-mail in error and that any use, dissemination, distribution, > forwarding, printing, or copying of this e-mail or any attached files > is strictly prohibited. If you have received this e-mail in error, > please immediately purge it and all attachments and notify the sender > by reply e-mail or contact the sender at the number listed. > > > From katri <@t> cogeco.ca Mon Jun 26 21:56:49 2006 From: katri <@t> cogeco.ca (Katri Tuomala) Date: Mon Jun 26 21:56:42 2006 Subject: [Histonet] Chromogens that survive HIER References: Message-ID: <003b01c69995$562e9cb0$6a9a9618@Katri> DAB would probably survive heat retrieval, but I don't have personal experience to that fact. Try it. Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "Rachael Emerson" To: Sent: Monday, June 26, 2006 3:06 PM Subject: [Histonet] Chromogens that survive HIER > Hello. I am working on a double stain, in which my first round of > developing > I use Vector Blue. > I then do a HIER and proceed.... > The Vector Blue stains beautifully and it remains the same even after > going > through heat induced antigen retrieval. > Can anyone tell me if there are other visualizations systems, besides > Vector > Blue, that can withstand going through HIER? > > Thank you > Rachael Emerson > > -- > Rachael L. Emerson > Center for Pediatric Biomedical Research > University of Rochester Medical Center > 575 Elmwood Avenue MRBX 1.11301 > Rochester, NY 14642 > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Tue Jun 27 05:39:06 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Jun 27 05:39:16 2006 Subject: [Histonet] salary for lab assistants References: Message-ID: <002701c699d5$eb629cb0$0b69ce44@yourxhtr8hvc4p> here we go. Last year when we gave out CAP lecture (well Hector did anyway),I were critiqued as having a bad attitude towards CAP. This is a perfect example why. Joe "Flame Me" Nocito alias "Joe The Toe" ----- Original Message ----- From: "Charles.Embrey" To: "Janice Mahoney" ; ; ; ; Sent: Monday, June 26, 2006 9:33 AM Subject: RE: [Histonet] salary for lab assistants First off, CAP doesn't exactly follow CLIA. CLIA is more stringent and what you describe is definitely considered grossing and "high complexity testing". CAP, on the other hand, is drawing a distinction between grossing (anything that must be cut in) and non-grossing (taking measurements and putting biopsies in a cassette with no cutting involved). Technically you can meet CAP standards while being in violation of CLIA regulations. If the lab does any billing with the government, (Medicare, Medicaid, Tricare) then you had better be meeting CLIA '88. Personally I thing CAP is so wrong for clouding the water on this one. Charles Embrey, PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Janice Mahoney Sent: Monday, June 26, 2006 8:24 AM To: Pathrm35@adelphia.net; pamm@hlclab.com; histonet@lists.utsouthwestern.edu; rjbuesa@yahoo.com Subject: RE: [Histonet] salary for lab assistants I completely agree. Weighing and measuring is grossing and the person who does so must qualify under CLIA (CAP follows CLIA). Many institutions are still allowing this grossing to be performed HT's and assistants who do not qualify. I have been performing CAP inspections for many years and have cited labs for this. I did my homework and have documentation from CLIA to substantiate the fact that "measuring, counting and weighing" is indeed grossing. Jan M. Omaha >>> "Ron Martin" 06/25/2006 11:21 AM >>> Should this lab assistant be "cassetting" at all? This sounds like a grossing tech to me and should fall under CLIA regs for high complex testing with the appropriate education/training. If this person isn't qualified should he/she be performing these duties at all? Ron Martin -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Sunday, June 25, 2006 9:38 AM To: Pam Mara; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] salary for lab assistants Pam: For your description your assistant works as a histotech although she may or may not have "academic qualifications". You have to consider also that initially histotechs were trained "on the job" and later on were "grandfathered" at licensure time (perhaps this is one of the reasons why historically histotechs are in lower echelon of clinical lab salary scale, but that is another subject to talk about!). I do not think that you should ask about lab aides paymen rates when your aide is performing tasks above those of that job position. As a lab supervisor/manager it should be part of your mission that of assuring that the employees you supervise are treated fairly. Having said all that I think that you should get for her a salary that reflects her tasks, not those usually corresponding for a job level. It is my opinion that she shoudl be paid, at least, at the histotech entry level. Hope this will help you! Ren? J. Pam Mara wrote: I have a question. We have a lab assistant here who does the "cassetting" of tissue specimens. She isn't a PA and she doesn't really gross in any large specimens. We mainly have GI specimens and biopsies, which she weighs/measures and puts into cassettes. She also helps with staining, coverslipping, etc. I was just wondering what is a reasonable salary for someone who performs these tasks? Does anyone else work with a lab assistant that performs similar tasks? Thanks for the info, Pam Mara -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Saturday, June 24, 2006 9:52 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 31, Issue 36 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Want to be your own boss? Learn how on Yahoo! Small Business. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.9.4/375 - Release Date: 6/25/2006 From cacartwr <@t> mdanderson.org Tue Jun 27 07:23:47 2006 From: cacartwr <@t> mdanderson.org (cacartwr@mdanderson.org) Date: Tue Jun 27 07:24:10 2006 Subject: [Histonet] destaining and DAB Message-ID: I have some slides that I did IHC on with DAB and then I counterstained with Hematoxylin. I want to destain the hematoxylin and re-do it. Will destaining affect the DAB? Thanks, Carrie From Charles.Embrey <@t> carle.com Tue Jun 27 08:07:38 2006 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Tue Jun 27 08:07:45 2006 Subject: [Histonet] (no subject) Message-ID: If they qualify under CLIA '88 as "High Complexity Testing Personnel" for CLIA and CAP inspection criteria they can. Even under the CAP guidelines this does meet the "grossing" definition because "dissection" is involved. Charles Embrey PA(ASCP) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Garcia, Amanda Sent: Monday, June 26, 2006 2:59 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) This question is related somewhat to the question of allowing a lab assistant to "cassette" tissue specimens. Where do qualification issues fall regarding weighing and measuring placentas and rolling the fetal membranes and cutting a piece and placing in a cassette? Is this ok for HT's to perform? > Amanda (Amy) Garcia > Histology/Pathology > College Station Medical Center > (979) 680-5372 office > (979) 696-5446 fax > *Mailto:amanda.garcia@triadhospitals.com > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the e-mail or any > of its attachments, please be advised that you have received this > e-mail in error and that any use, dissemination, distribution, > forwarding, printing, or copying of this e-mail or any attached files > is strictly prohibited. If you have received this e-mail in error, > please immediately purge it and all attachments and notify the sender > by reply e-mail or contact the sender at the number listed. > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histosci <@t> shentel.net Tue Jun 27 08:44:44 2006 From: histosci <@t> shentel.net (HSRL) Date: Tue Jun 27 08:45:00 2006 Subject: [Histonet] Air Purifier for Xylene Message-ID: <001201c699ef$dc457210$0500a8c0@HSRLMAIN> Dear Netters, Has anyone used an air purifier made specifically for formaldehyde and xylene fumes? I know Newcomer Supply has started distributing the system. Does anyone have any feedback on the Newcomer system or any other air purifying system? Thanks for your feedback, Tom Galati Laboratory Director HSRL, Inc.- A GLP Compliant Contract Laboratory 5930 Main Street Mount Jackson, Virginia 22842 540.477.4440 Fax: 540.477.4448 tomgalati@hsrl.org www.hsrl.org From pmarcum <@t> vet.upenn.edu Tue Jun 27 08:47:46 2006 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Tue Jun 27 08:47:58 2006 Subject: [Histonet] Need Some Help or Information Message-ID: <6.1.1.1.2.20060627094347.019c12d8@mail.vet.upenn.edu> Good Morning All, I have a question for one of our PIs here. She wants to do 3mm sections of cadaver leg for anatomic and radiographic study. She wants to do either cryogenic or plastization of the leg with a latex fill of the veins and arteries. Thanks for any information or direction on this. I will forward to the PI or discuss it with her. Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From kgrobert <@t> rci.rutgers.edu Tue Jun 27 09:15:11 2006 From: kgrobert <@t> rci.rutgers.edu (Kathleen Roberts) Date: Tue Jun 27 09:14:12 2006 Subject: [Histonet] Faded acid fuschin slides Message-ID: <44A13D6F.8050702@rci.rutgers.edu> I need to restain some slides that had been stained with acid fuschin, and I am wondering if anyone here has done this. I did a search of Histonet for this and didn't find anything specific for acid fuschin. Would someone out there happen to have a protocol? Thanks in advance, Kathleen Principal Lab Technician Neurotoxicology Laboratories Dept of Pharmacology and Toxicology Ernest Mario School of Pharmacy Rutgers, the State University of NJ From laurie.colbert <@t> huntingtonhospital.com Tue Jun 27 09:27:32 2006 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Tue Jun 27 09:27:40 2006 Subject: [Histonet] Air Purifier for Xylene Message-ID: <0BE6ADFAE4E7E04496BF21ABD346628005653C01@EXCHANGE1.huntingtonhospital.com> Creative Waste Solutions has an fume controller that works well for us for eliminating formalin fumes. It is supposed to work well for xylene fumes as well. Their phone number is (888) 795-8300. Laurie Colbert Huntington Hospital -----Original Message----- From: HSRL [mailto:histosci@shentel.net] Sent: Tuesday, June 27, 2006 6:45 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Air Purifier for Xylene Dear Netters, Has anyone used an air purifier made specifically for formaldehyde and xylene fumes? I know Newcomer Supply has started distributing the system. Does anyone have any feedback on the Newcomer system or any other air purifying system? Thanks for your feedback, Tom Galati Laboratory Director HSRL, Inc.- A GLP Compliant Contract Laboratory 5930 Main Street Mount Jackson, Virginia 22842 540.477.4440 Fax: 540.477.4448 tomgalati@hsrl.org www.hsrl.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Jun 27 10:15:43 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jun 27 10:15:52 2006 Subject: [Histonet] (no subject) In-Reply-To: <8B63039C9DF4554C8FDBF31235F0E1480193E75B@CPRTEVS02.triadhospitals.net> Message-ID: <20060627151543.27662.qmail@web61214.mail.yahoo.com> Amanda: This is how I feel about it: along the workflow in histology perhaps the position with the highest judgemental decision level is the one corresponding to the MACROSCOPIC selection of a piece of tissue for diagnostic purposes. Whenever somebody has to judge, evaluate and decide what to process for diagnosis that somebody has to have not only knowledge and experience but great responsibility. Any action that resembles this level of decision making should be reserved to well trained personnel like Pathologists, Pathology Residents and Pathology Assistants. In my view point nobody else should be allowed to take the decision of selecting one piece of tissue over another for diagnosis. This step in the workflow could lead to the worst type of diagnostic result, the FALSE NEGATIVE that, in my personal opinion, is the worst wrong diagnosis. In your question you cannot be sure if there is some macroscopic pathology that could end unidentified by the histotech. Unless the whole sample is put through to process, the histotechs should not be involved in any step dealing with selecting a part of the sample over another to be processed. This is just my opinion and hope it will help you in your question. Ren? J. "Garcia, Amanda" wrote: This question is related somewhat to the question of allowing a lab assistant to "cassette" tissue specimens. Where do qualification issues fall regarding weighing and measuring placentas and rolling the fetal membranes and cutting a piece and placing in a cassette? Is this ok for HT's to perform? > Amanda (Amy) Garcia > Histology/Pathology > College Station Medical Center > (979) 680-5372 office > (979) 696-5446 fax > *Mailto:amanda.garcia@triadhospitals.com > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the e-mail or any > of its attachments, please be advised that you have received this > e-mail in error and that any use, dissemination, distribution, > forwarding, printing, or copying of this e-mail or any attached files > is strictly prohibited. If you have received this e-mail in error, > please immediately purge it and all attachments and notify the sender > by reply e-mail or contact the sender at the number listed. > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Sneak preview the all-new Yahoo.com. It's not radically different. Just radically better. From PMonfils <@t> Lifespan.org Tue Jun 27 10:31:12 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Tue Jun 27 10:31:18 2006 Subject: [Histonet] destaining and DAB Message-ID: <09C945920A6B654199F7A58A1D7D1FDE01717747@lsexch.lsmaster.lifespan.org> Destaining the hematoxylin (with acid alcohol?) won't alter the DAB staining. DAB staining is extremely stable, and difficult to remove chemically even when you want to. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > cacartwr@mdanderson.org > Sent: Tuesday, June 27, 2006 5:23 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] destaining and DAB > > I have some slides that I did IHC on with DAB and then I counterstained > with Hematoxylin. I want to destain the hematoxylin and re-do it. Will > destaining affect the DAB? > Thanks, > Carrie > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From jennifer.l.hofecker <@t> Vanderbilt.Edu Tue Jun 27 10:28:53 2006 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Tue Jun 27 10:32:25 2006 Subject: [Histonet] Air Purifier for Xylene References: <001201c699ef$dc457210$0500a8c0@HSRLMAIN> Message-ID: <898D946569A27444B65667A49C074052852D91@mailbe06.mc.vanderbilt.edu> We have the Newcomer purifier for Formaldehyde and xylene. It has been working well for us. People used to walk in and say they could smell xylene (even though we were well under STEL and PEL). Since installing the filter, those same people come in and say nothing. Also, our industrial hygiene person was in here looking at it and said it looked like a very good one. The only problem may be finding a place to put it. Ours is currently sitting in the middle of the lab like an island. Good luck and enjoy the clean air. Jennifer Hofecker, HT (ASCP) Vanderbilt University Medical Center Division of Neuropathology (615) 343-0083 (615) 343-7089 fax ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of HSRL Sent: Tue 6/27/2006 8:44 AM To: histonet@pathology.swmed.edu Subject: [Histonet] Air Purifier for Xylene Dear Netters, Has anyone used an air purifier made specifically for formaldehyde and xylene fumes? I know Newcomer Supply has started distributing the system. Does anyone have any feedback on the Newcomer system or any other air purifying system? Thanks for your feedback, Tom Galati Laboratory Director HSRL, Inc.- A GLP Compliant Contract Laboratory 5930 Main Street Mount Jackson, Virginia 22842 540.477.4440 Fax: 540.477.4448 tomgalati@hsrl.org www.hsrl.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mcauliff <@t> umdnj.edu Tue Jun 27 10:16:01 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Tue Jun 27 11:16:09 2006 Subject: [Histonet] Need Some Help or Information In-Reply-To: <6.1.1.1.2.20060627094347.019c12d8@mail.vet.upenn.edu> References: <6.1.1.1.2.20060627094347.019c12d8@mail.vet.upenn.edu> Message-ID: <44A14BB1.8010106@umdnj.edu> Hi Pam: Freeze the lower extremity and cut it with a band saw. Really. That is how we did it when sectioning a whole cadaver. A company called "Sarcosote" on Old Harrods Creek Road in Louisville, KY (actually in Anchorage, KY), 502-244-9133 might be able to help you. Geoff Pamela Marcum wrote: > > > Good Morning All, > > I have a question for one of our PIs here. She wants to do 3mm > sections of cadaver leg for anatomic and radiographic study. She > wants to do either cryogenic or plastization of the leg with a latex > fill of the veins and arteries. Thanks for any information or > direction on this. I will forward to the PI or discuss it with her. > > > Best Regards, > > Pamela A Marcum > Manager, Histology Special Procedures > University of Pennsylvania > School of Veterinary Medicine > R.S. Reynolds Jr. CORL > New Bolton Center > 382 West Street Road > Kennett Square, PA 19348 > > Phone - 610-925-6278 > Fax - 610-925-8120 > E-mail - pmarcum@vet.upenn.edu > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From histology <@t> gradymem.org Tue Jun 27 11:42:47 2006 From: histology <@t> gradymem.org (histology@gradymem.org) Date: Tue Jun 27 11:43:04 2006 Subject: [Histonet] (no subject) Message-ID: I had the same kind of questions and I talked to a CLIA person on the phone. It was explained to me that if your measurements and descriptions becomes part of the pathology report, then you are doing complex testing. So, if all you do is measure or weight a specimen and put the whole thing in a cassette you must be qualified to do so by CLIA standards. Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org ----- Original Message ----- From: "Garcia, Amanda" Date: Monday, June 26, 2006 2:58 pm Subject: [Histonet] (no subject) > > This question is related somewhat to the question of allowing a lab > assistant to "cassette" tissue specimens. Where do qualification > issuesfall regarding weighing and measuring placentas and rolling > the fetal > membranes and cutting a piece and placing in a cassette? Is this > ok for > HT's to perform? > > Amanda (Amy) Garcia > > Histology/Pathology > > College Station Medical Center > > (979) 680-5372 office > > (979) 696-5446 fax > > *Mailto:amanda.garcia@triadhospitals.com > > > > This email and any files transmitted with it may contain > PRIVILEGED or > > CONFIDENTIAL information and may be read or used only by the > intended> recipient. If you are not the intended recipient of the > e-mail or any > > of its attachments, please be advised that you have received this > > e-mail in error and that any use, dissemination, distribution, > > forwarding, printing, or copying of this e-mail or any attached > files> is strictly prohibited. If you have received this e-mail in > error,> please immediately purge it and all attachments and notify > the sender > > by reply e-mail or contact the sender at the number listed. > > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From detmar <@t> mshri.on.ca Tue Jun 27 12:31:37 2006 From: detmar <@t> mshri.on.ca (Jacqui Detmar) Date: Tue Jun 27 12:31:51 2006 Subject: [Histonet] Chromogens that survive HIER Message-ID: Hi there. I have done HIER (citrate buffer, pH 6) on DAB-stained mouse placentae and it works great. Jacqui Detmar -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rachael Emerson Sent: Monday, June 26, 2006 3:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Chromogens that survive HIER Hello. I am working on a double stain, in which my first round of developing I use Vector Blue. I then do a HIER and proceed.... The Vector Blue stains beautifully and it remains the same even after going through heat induced antigen retrieval. Can anyone tell me if there are other visualizations systems, besides Vector Blue, that can withstand going through HIER? Thank you Rachael Emerson -- Rachael L. Emerson Center for Pediatric Biomedical Research University of Rochester Medical Center 575 Elmwood Avenue MRBX 1.11301 Rochester, NY 14642 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From soofias2 <@t> yahoo.com Tue Jun 27 12:55:26 2006 From: soofias2 <@t> yahoo.com (soofia siddiqui) Date: Tue Jun 27 12:55:31 2006 Subject: [Histonet] Any suggestion on Promega or Chemicon TUNEL System on human tissue. Message-ID: <20060627175526.25340.qmail@web39504.mail.mud.yahoo.com> I am going to start Staining on human skin Using TUNEL System. I wanted to get some information about the vender if someone have succesfully used any system on human tissue.I am looking at Promega and Chemicon but really want to get some feed back if someone have used any of them or some other . ss --------------------------------- Yahoo! Sports Fantasy Football ?06 - Go with the leader. Start your league today! From skmak1886 <@t> yahoo.com Tue Jun 27 12:57:35 2006 From: skmak1886 <@t> yahoo.com (Sally Mak) Date: Tue Jun 27 13:06:12 2006 Subject: [Histonet] lipofuscin detection Message-ID: <20060627175735.97203.qmail@web53305.mail.yahoo.com> I would like to know how to detect the lipofuscin in the frozen fixed brain tissue. I tried carbol fuchsin solution but it seems not so good at all. Thanks for sharing. --------------------------------- Yahoo! Messenger with Voice. PC-to-Phone calls for ridiculously low rates. From RSRICHMOND <@t> aol.com Tue Jun 27 13:46:05 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Tue Jun 27 13:46:16 2006 Subject: [Histonet] who grosses and embeds? Message-ID: <4ff.1ccebd8.31d2d6ed@aol.com> The question of who is allowed to gross what surgical pathology specimens seems to keep coming up. Right now the regulatory agencies seem to be leaning toward making the pathologist do it all. In contrast, nobody seems to be interested in the embedder - who in effect re-grosses small biopsy specimens. When I was a resident (around 1970) we used to fill out a sheet - listing the number of biopsy specimens for example - that the embedder consulted while embedding. I've worked in maybe forty surgical pathology services since then (as a locum tenens pathologist, mostly) and I don't think I've ever seen an embedding sheet again. It's quite disconcerting when the endoscopist describes three specimens, I receive two, and the next day there's only one piece of tissue on the slide. It seems to me that the College of American Pathologists (CAP) should make it a Phase II (immediate remediation required) to embed small specimens without an embedding sheet. Cowpath, Tantrum, and other surgical pathology data bases should be able to generate a compact case list that the count of specimens could be handwritten onto - Cowpath at least requires that a compact specimen log be handwritten. And pathology residents should be required to come in during the wee hours and learn how to embed! Bob Richmond Knoxville TN and Gastonia NC From JWEEMS <@t> sjha.org Tue Jun 27 13:54:27 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Jun 27 13:53:21 2006 Subject: [Histonet] who grosses and embeds? Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202960104@sjhaexc02.sjha.org> Well said. We would pass on that requirement as we do still count pieces - written on the requisition for the transcriptionist to put in the gross template, and transferred to the Triple G system for the embedders. If we don't have the correct number of pieces, it is brought to the attention of the pathologist. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of RSRICHMOND@aol.com Sent: Tuesday, June 27, 2006 2:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] who grosses and embeds? The question of who is allowed to gross what surgical pathology specimens seems to keep coming up. Right now the regulatory agencies seem to be leaning toward making the pathologist do it all. In contrast, nobody seems to be interested in the embedder - who in effect re-grosses small biopsy specimens. When I was a resident (around 1970) we used to fill out a sheet - listing the number of biopsy specimens for example - that the embedder consulted while embedding. I've worked in maybe forty surgical pathology services since then (as a locum tenens pathologist, mostly) and I don't think I've ever seen an embedding sheet again. It's quite disconcerting when the endoscopist describes three specimens, I receive two, and the next day there's only one piece of tissue on the slide. It seems to me that the College of American Pathologists (CAP) should make it a Phase II (immediate remediation required) to embed small specimens without an embedding sheet. Cowpath, Tantrum, and other surgical pathology data bases should be able to generate a compact case list that the count of specimens could be handwritten onto - Cowpath at least requires that a compact specimen log be handwritten. And pathology residents should be required to come in during the wee hours and learn how to embed! Bob Richmond Knoxville TN and Gastonia NC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From bjdewe <@t> aol.com Tue Jun 27 14:09:48 2006 From: bjdewe <@t> aol.com (bjdewe@aol.com) Date: Tue Jun 27 14:09:56 2006 Subject: [Histonet] IHC Workshop in California Message-ID: <8C868346502B3F2-1BB0-DB7@mblk-d36.sysops.aol.com> We still have a few spots left for our IHC on Animal Tissue workshop at UC Davis on July 28th. Here is a link to our flyer. http://www.vetmed.ucdavis.edu/vsr/dil/events.html To register go to: www.innvx.com and click on workshop on the home page... If you have any questions please ask;-) Cheers, Loralei Dewe Live as if you were to die tomorrow. Learn as if you were to live forever. - Gandhi - ******************************************************************* Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s)and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ******************************************************************* ________________________________________________________________________ Check out AOL.com today. Breaking news, video search, pictures, email and IM. All on demand. Always Free. From rjbuesa <@t> yahoo.com Tue Jun 27 14:51:41 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jun 27 14:51:47 2006 Subject: [Histonet] who grosses and embeds? In-Reply-To: <4ff.1ccebd8.31d2d6ed@aol.com> Message-ID: <20060627195141.6616.qmail@web61225.mail.yahoo.com> The number of biopsy pieces in a log form to be consulted by the embedding HT is standard proceure at Mount Sinai Medical Center (MIami Beach), Ohio State University and Quest Diagnostics (South Florida). Ren? J. RSRICHMOND@aol.com wrote: The question of who is allowed to gross what surgical pathology specimens seems to keep coming up. Right now the regulatory agencies seem to be leaning toward making the pathologist do it all. In contrast, nobody seems to be interested in the embedder - who in effect re-grosses small biopsy specimens. When I was a resident (around 1970) we used to fill out a sheet - listing the number of biopsy specimens for example - that the embedder consulted while embedding. I've worked in maybe forty surgical pathology services since then (as a locum tenens pathologist, mostly) and I don't think I've ever seen an embedding sheet again. It's quite disconcerting when the endoscopist describes three specimens, I receive two, and the next day there's only one piece of tissue on the slide. It seems to me that the College of American Pathologists (CAP) should make it a Phase II (immediate remediation required) to embed small specimens without an embedding sheet. Cowpath, Tantrum, and other surgical pathology data bases should be able to generate a compact case list that the count of specimens could be handwritten onto - Cowpath at least requires that a compact specimen log be handwritten. And pathology residents should be required to come in during the wee hours and learn how to embed! Bob Richmond Knoxville TN and Gastonia NC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. From SHARON.OSBORN <@t> SPCORP.COM Tue Jun 27 16:55:25 2006 From: SHARON.OSBORN <@t> SPCORP.COM (Osborn, Sharon) Date: Tue Jun 27 16:55:36 2006 Subject: [Histonet] RE: Air purifier Message-ID: <9A919A5D70313A4D9C56A025710874080C731D@kenmsg40.us.schp.com> Tom, For over two years now, we have had "R2D2" sitting on the floor of our lab so it can circulate the air while removing the wonderful perfumes that histology is well known for. The comments/complaints have decreased so much that other departments are clamoring for our $1500.00 solution. Additional filters are $449 each. We got this through ml-MarketLab JL9390 for the Air Filtration System with Filter. Replacement Filter is JL9266. www.MarketLabInc.com or 1.800.237.3604. Now, we very rarely have a complaint from someone about the odors; so that says it is working well. We leave it on 24/7 at the medium setting. The high setting stirs up too much dust for me allergies. sharon osborn DNAX, SP BioPharma Palo Alto, CA Date: Tue, 27 Jun 2006 09:44:44 -0400 From: "HSRL" Subject: [Histonet] Air Purifier for Xylene To: Message-ID: <001201c699ef$dc457210$0500a8c0@HSRLMAIN> Content-Type: text/plain; charset="us-ascii" Dear Netters, Has anyone used an air purifier made specifically for formaldehyde and xylene fumes? I know Newcomer Supply has started distributing the system. Does anyone have any feedback on the Newcomer system or any other air purifying system? Thanks for your feedback, Tom Galati Laboratory Director HSRL, Inc.- A GLP Compliant Contract Laboratory 5930 Main Street Mount Jackson, Virginia 22842 540.477.4440 Fax: 540.477.4448 tomgalati@hsrl.org www.hsrl.org ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From kemlo.rogerson <@t> waht.swest.nhs.uk Wed Jun 28 03:30:26 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Jun 28 03:30:06 2006 Subject: [Histonet] who grosses and embeds? Message-ID: IMHO those who gross and embed should be those with the competency and training to do it; it could be a Pathologists, he/she could delegate to a SR or 'Junior Medic' that they have trained or it could be delegated to a BMS (Histotech) who is qualified (Specialist Exam) and competent. I have personally grossed skin biopsies, uterus, dead foals and budgies. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 People are like stained glass windows: they sparkle and shine when the sun is out, but when the darkness sets in their true beauty is revealed only if there is a light within. --Elizabeth Kubler-Ross This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From jnocito <@t> satx.rr.com Wed Jun 28 05:47:46 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Jun 28 05:49:20 2006 Subject: [Histonet] who grosses and embeds? References: <4ff.1ccebd8.31d2d6ed@aol.com> Message-ID: <006001c69aa0$4b1d15c0$0b69ce44@yourxhtr8hvc4p> Dr. R, you bring up an excellent point. We have one pathologist that refuses to cut shave bxs, vas deferens, fallopian tubes, temporal arteries and other small specimens. I have instructed my embedders that they have to cut these specimens for proper orientation. Is this considered grossing? As far as grossing sheets, every lab that I have worked in, plus the lab that I started, always had a grossing sheet. It's almost mal-practice not to. How could you tell if a specimen has special instructions or not such as a renal bx? Just my 3 cents worth. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: To: Sent: Tuesday, June 27, 2006 1:46 PM Subject: [Histonet] who grosses and embeds? > The question of who is allowed to gross what surgical pathology specimens > seems to keep coming up. Right now the regulatory agencies seem to be > leaning > toward making the pathologist do it all. > > In contrast, nobody seems to be interested in the embedder - who in effect > re-grosses small biopsy specimens. When I was a resident (around 1970) we > used > to fill out a sheet - listing the number of biopsy specimens for example - > that > the embedder consulted while embedding. I've worked in maybe forty > surgical > pathology services since then (as a locum tenens pathologist, mostly) and > I > don't think I've ever seen an embedding sheet again. > > It's quite disconcerting when the endoscopist describes three specimens, I > receive two, and the next day there's only one piece of tissue on the > slide. > > It seems to me that the College of American Pathologists (CAP) should make > it > a Phase II (immediate remediation required) to embed small specimens > without > an embedding sheet. > > Cowpath, Tantrum, and other surgical pathology data bases should be able > to > generate a compact case list that the count of specimens could be > handwritten > onto - Cowpath at least requires that a compact specimen log be > handwritten. > > And pathology residents should be required to come in during the wee hours > and learn how to embed! > > Bob Richmond > Knoxville TN and Gastonia NC > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.394 / Virus Database: 268.9.5/376 - Release Date: 6/26/2006 > > From Terry.Marshall <@t> rothgen.nhs.uk Wed Jun 28 06:19:08 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Wed Jun 28 06:16:09 2006 Subject: [Histonet] who grosses and embeds? Message-ID: So, you got a feather in your cap after flogging a dead horse. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:kemlo.rogerson@waht.swest.nhs.uk] Sent: 28 June 2006 09:30 To: 'Rene J Buesa'; RSRICHMOND@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] who grosses and embeds? IMHO those who gross and embed should be those with the competency and training to do it; it could be a Pathologists, he/she could delegate to a SR or 'Junior Medic' that they have trained or it could be delegated to a BMS (Histotech) who is qualified (Specialist Exam) and competent. I have personally grossed skin biopsies, uterus, dead foals and budgies. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 People are like stained glass windows: they sparkle and shine when the sun is out, but when the darkness sets in their true beauty is revealed only if there is a light within. --Elizabeth Kubler-Ross This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From einar.jorundsson <@t> decode.is Wed Jun 28 07:49:22 2006 From: einar.jorundsson <@t> decode.is (Einar =?ISO-8859-1?Q?J=F6rundsson?=) Date: Wed Jun 28 07:49:27 2006 Subject: [Histonet] CD68 in FFPE mouse tissue Message-ID: I am having problems with IHC staining with macrophage-markers in formalin-fixed paraffin-embedded (FFPE) mouse arotic root sections. F4/80 (Serotec) works well in spleen (after proteinase K digestion) but is inconsistent in aortas. I have also tried CD68 clone FA11 (Serotec) with proteinase K digestion and HIER (citrate, borate, Tris-HCl) with no success. Any tips? Regards, Einar. From Susan.Ferrigon <@t> sanofi-aventis.com Wed Jun 28 08:00:28 2006 From: Susan.Ferrigon <@t> sanofi-aventis.com (Susan.Ferrigon@sanofi-aventis.com) Date: Wed Jun 28 08:00:47 2006 Subject: [Histonet] Anti Glycine Transporter 1 Message-ID: <90B6684A9D6DAF468F7A5DC148754E1D2154D4@ALPW31.f2.enterprise> Hi Does anyone have any experience/Protocols for ICC staining with Anti-Glycine Transporter 1 on rat Brain/Liver This is a new one for me and have been asked if I can try to get a method working pretty quickly, so if anyone has any experience they could share I would be really grateful. Thanks Susan From LRaff <@t> lab.uropartners.com Wed Jun 28 08:34:03 2006 From: LRaff <@t> lab.uropartners.com (Lester Raff) Date: Wed Jun 28 08:34:08 2006 Subject: [Histonet] Dako Autostainer Message-ID: <5DA1CA5D0B98A84985B545A24423B822A501@UPLAB01.uplab.local> Hello, and thanks to all for their input on the PASCAL pressure cooker. Another IHC question. We use the Dako Autostainer Plus and occassionaly have areas on the slides that don't seem to stain. We are careful not to let the slides dry out. Do Autostainers have a problem distributing reagents evenly? Thanks, Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory ph: 708-486-0076 fax: 708-486-0080 From rjbuesa <@t> yahoo.com Wed Jun 28 08:48:52 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jun 28 08:48:58 2006 Subject: [Histonet] Dako Autostainer In-Reply-To: <5DA1CA5D0B98A84985B545A24423B822A501@UPLAB01.uplab.local> Message-ID: <20060628134852.21343.qmail@web61214.mail.yahoo.com> They should not but if you are diluting concentrated Abs you should remember to add Tween 20 to assure even distribution. Ren? J. Lester Raff wrote: Hello, and thanks to all for their input on the PASCAL pressure cooker. Another IHC question. We use the Dako Autostainer Plus and occassionaly have areas on the slides that don't seem to stain. We are careful not to let the slides dry out. Do Autostainers have a problem distributing reagents evenly? Thanks, Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory ph: 708-486-0076 fax: 708-486-0080 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. From Dorothy.L.Webb <@t> HealthPartners.Com Wed Jun 28 09:43:06 2006 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Wed Jun 28 09:43:14 2006 Subject: [Histonet] Davidson's Message-ID: <0E394B648E5284478A6CCB78E5AFDA270171FE69@hpes1.HealthPartners.int> Has anyone had any problems with Davidson's fixative interfering with their IHC staining? We have not run any comparison studies, but, one of our pathologists heard "whispers" of this amongst fellow pathologists at another facility!! Any thoughts on this would be appreciated!! From Charlene.Henry <@t> STJUDE.ORG Wed Jun 28 10:38:22 2006 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Wed Jun 28 10:42:18 2006 Subject: [Histonet] Any suggestion on Promega or Chemicon TUNEL System onhuman tissue.. . Message-ID: <5CB39BCA5724F349BCB748675C6CA1A2099A1CFE@SJMEMXMB02.stjude.sjcrh.local> We use the Promega Dead End kit and it works great; however when we do a TUNEL assay, we also run pHistone IHC. Charlene Henry HT (ASCP), QIHC Histology/Immunohistochemistry Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of soofia siddiqui Sent: Tuesday, June 27, 2006 12:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Any suggestion on Promega or Chemicon TUNEL System onhuman tissue.. . I am going to start Staining on human skin Using TUNEL System. I wanted to get some information about the vender if someone have succesfully used any system on human tissue.I am looking at Promega and Chemicon but really want to get some feed back if someone have used any of them or some other . ss --------------------------------- Yahoo! Sports Fantasy Football '06 - Go with the leader. Start your league today! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Wed Jun 28 10:46:07 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Wed Jun 28 10:46:17 2006 Subject: [Histonet] who grosses and embeds? Message-ID: <53c.19c47aa.31d3fe3f@aol.com> Kemlo Rogerson notes: >>I have personally grossed skin biopsies, uterus, dead foals and budgies.<< Presumably not John Lennon's budgie - I have a little budgie he is my very pal I take him walks in Britain I hope I always shall. Bob Richmond From Pathrm35 <@t> adelphia.net Wed Jun 28 11:04:27 2006 From: Pathrm35 <@t> adelphia.net (Ron Martin) Date: Wed Jun 28 11:04:27 2006 Subject: [Histonet] need safety manual info Message-ID: <000401c69acc$88789e90$2ea2ac45@D6WRV2B1> If anyone is willing to share some info about their safety manual, I would appreciate it. I need info on: chemical hygiene plans, ergonomics and OSHA regulations. Thanks in advance Ron Martin From TMcNemar <@t> lmhealth.org Wed Jun 28 11:33:32 2006 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Wed Jun 28 11:34:03 2006 Subject: [Histonet] Dako Autostainer Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F3D8@lmhsmail.lmhealth.org> What counterstain do you use? Hematoxylin will clumb and clog the probe causing uneven staining. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Lester Raff Sent: Wednesday, June 28, 2006 9:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dako Autostainer Hello, and thanks to all for their input on the PASCAL pressure cooker. Another IHC question. We use the Dako Autostainer Plus and occassionaly have areas on the slides that don't seem to stain. We are careful not to let the slides dry out. Do Autostainers have a problem distributing reagents evenly? Thanks, Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory ph: 708-486-0076 fax: 708-486-0080 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From themagoos <@t> rushmore.com Wed Jun 28 11:22:18 2006 From: themagoos <@t> rushmore.com (themagoos@rushmore.com) Date: Wed Jun 28 11:39:34 2006 Subject: [Histonet] TBS Microwave Programs Message-ID: <1151511738.44a2acba352de@webmail.rushmore.com> We have a TBS Shurwave 1200 and we are trying to process fatty skin specimens. Does anybody have a sample program for microwave processing fatty skin specimens? We are also looking at microwave processing all types of specimens. Any feedback would be appreciated. Thank you. Jason McGough HT(ASCP) Clinical LAboratory of the Black Hills 2805 5th Street Rapid City, SD 57701 605-343-2267 ------------------------------------------------- This mail sent by http://webmail.rushmore.com From HerberDL <@t> moffitt.usf.edu Wed Jun 28 12:54:54 2006 From: HerberDL <@t> moffitt.usf.edu (Herber, Donna L.) Date: Wed Jun 28 12:55:20 2006 Subject: [Histonet] Gr-1 staining in mouse spleen Message-ID: <21E81F01D3A58943AD79F8753AEF65A24A7442@M-EXVMB2.hlm.ad.moffitt.usf.edu> I have gotten wonderfully accurate information from histonet in the past! Now, I have been trying for Gr-1 staining of mouse spleen for weeks. The best result have come only by direct application of the BD antibody at 0.5mg/mL and the results look like all of the white pulp cells are positive. Naive mice and tumor bearing mice look identical. There is nothing on my "no primary" controls. However, I doubt this staining is specific. My protocol is as follows: * Thaw slide mounted frozen sections (in foil pack), air dry, and fix in acetone 10 minutes at RT. * Air dry and apply PAP pen * Hydrate in PBS (and removes embedding medium) the rest of the protocol at RT * Block endogenous peroxidase with 1:1:8 methanol:30% H2O2:PBS for 15 min. * Wash 3x PBS 5 min * Permeabilize 30 min with 1.8% lysine. 0.2% triton X-100, 4% serum, in PBS * Quick rinse in PBS * Direct application of antibody BD# 553123 incubate overnight. * Wash 3x PBS 5 min * Vector biotinylated secondary #BA4001 rabbit anti-rat, 1:1000 dilution, 2 hours * Wash 3x PBS 5 min * Vectastain ABC kit PK-4000 one hour * Wash 2x PBS, 1x TBS, 5 min * DAB 0.05% + nickelous ammonium sulfate 0.5% in TBS 5 min * Add 0.1% of 30% H2O2 for 5 min to develop black color * wash, dehydrate, coverslip with DPX I have tried the Serotec product as well as the BD IHC product for Gr-1, and various dilutions of primary to no avail. Note that I get excellent T-cell staining with CD4 and CD8a with this same protocol, with similar distribution to the white pulp. I have not found many examples of Gr-1 IHC in the literature. Has anyone performed this staining routinely and what type of distribution do you see? Any comments or suggestions would be greatly appreciated. Donna Herber, Ph.D. Post Doctoral Scholar Immunology (Gabrilovich Laboratory) H. Lee Moffitt Cancer Center and Research Institute 745-6097 Donna Herber, Ph.D. Post Doctoral Scholar Immunology (Gabrilovich Laboratory) H. Lee Moffitt Cancer Center and Research Institute 745-6097 ----------------------------------------- ################################################################### ########## This transmission may be confidential or protected from disclosure and is only for review and use by the intended recipient. Access by anyone else is unauthorized. Any unauthorized reader is hereby notified that any review, use, dissemination, disclosure or copying of this information, or any act or omission taken in reliance on it, is prohibited and may be unlawful. If you received this transmission in error, please notify the sender immediately. Thank you. ################################################################### ########## From Charlotte.Kopczynski <@t> baycare.org Wed Jun 28 13:10:12 2006 From: Charlotte.Kopczynski <@t> baycare.org (Kopczynski, Charlotte) Date: Wed Jun 28 13:07:28 2006 Subject: [Histonet] Embedding Work Sheets Message-ID: We currently use Cerner Millennium and after the cases have been accessioned, we print a Grossing Worklist which has several lines between cases. This is used at the Grossing Bench where Block/pieces and any special instructions are written manually. Then the Processing tasks are entered into Cerner which has a field for pieces for each processing task (H&E Block). After all the grossing info has been entered, an embedding worklist is printed along with slide labels. The person embedding, checks the worklist and initials each case. If any discrepancies occur, the embedder follows up with the Pathologist or PA. When the slides are stained, coverslipped and labeled, the blocks are checked against the slides and the worklist is initialed before delivery to the pathologist. Thanks, Charlotte Kopczynski,HTL(ASCP) Baycare Pathology Manager Phone: 727-461-8246 Fax: 727-462-7597 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, June 28, 2006 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 31, Issue 40 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Chromogens that survive HIER (Jacqui Detmar) 2. Any suggestion on Promega or Chemicon TUNEL System on human tissue. (soofia siddiqui) 3. lipofuscin detection (Sally Mak) 4. who grosses and embeds? (RSRICHMOND@aol.com) 5. RE: who grosses and embeds? (Weems, Joyce) 6. IHC Workshop in California (bjdewe@aol.com) 7. Re: who grosses and embeds? (Rene J Buesa) 8. RE: Air purifier (Osborn, Sharon) 9. RE: who grosses and embeds? (Kemlo Rogerson) 10. Re: who grosses and embeds? (Joe Nocito) 11. RE: who grosses and embeds? (Marshall Terry Dr, Consultant Histopathologist) 12. CD68 in FFPE mouse tissue (Einar J?rundsson) 13. Anti Glycine Transporter 1 (Susan.Ferrigon@sanofi-aventis.com) 14. Dako Autostainer (Lester Raff) 15. Re: Dako Autostainer (Rene J Buesa) 16. Davidson's (Webb, Dorothy L) 17. RE: Any suggestion on Promega or Chemicon TUNEL System onhuman tissue.. . (Henry, Charlene) 18. Re: who grosses and embeds? (RSRICHMOND@aol.com) 19. need safety manual info (Ron Martin) 20. RE: Dako Autostainer (Tom McNemar) 21. TBS Microwave Programs (themagoos@rushmore.com) ---------------------------------------------------------------------- Message: 1 Date: Tue, 27 Jun 2006 13:31:37 -0400 From: "Jacqui Detmar" Subject: RE: [Histonet] Chromogens that survive HIER To: "Rachael Emerson" , Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi there. I have done HIER (citrate buffer, pH 6) on DAB-stained mouse placentae and it works great. Jacqui Detmar -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rachael Emerson Sent: Monday, June 26, 2006 3:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Chromogens that survive HIER Hello. I am working on a double stain, in which my first round of developing I use Vector Blue. I then do a HIER and proceed.... The Vector Blue stains beautifully and it remains the same even after going through heat induced antigen retrieval. Can anyone tell me if there are other visualizations systems, besides Vector Blue, that can withstand going through HIER? Thank you Rachael Emerson -- Rachael L. Emerson Center for Pediatric Biomedical Research University of Rochester Medical Center 575 Elmwood Avenue MRBX 1.11301 Rochester, NY 14642 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Tue, 27 Jun 2006 10:55:26 -0700 (PDT) From: soofia siddiqui Subject: [Histonet] Any suggestion on Promega or Chemicon TUNEL System on human tissue. To: histonet@lists.utsouthwestern.edu Message-ID: <20060627175526.25340.qmail@web39504.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I am going to start Staining on human skin Using TUNEL System. I wanted to get some information about the vender if someone have succesfully used any system on human tissue.I am looking at Promega and Chemicon but really want to get some feed back if someone have used any of them or some other . ss --------------------------------- Yahoo! Sports Fantasy Football '06 - Go with the leader. Start your league today! ------------------------------ Message: 3 Date: Tue, 27 Jun 2006 10:57:35 -0700 (PDT) From: Sally Mak Subject: [Histonet] lipofuscin detection To: histonet@lists.utsouthwestern.edu Message-ID: <20060627175735.97203.qmail@web53305.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I would like to know how to detect the lipofuscin in the frozen fixed brain tissue. I tried carbol fuchsin solution but it seems not so good at all. Thanks for sharing. --------------------------------- Yahoo! Messenger with Voice. PC-to-Phone calls for ridiculously low rates. ------------------------------ Message: 4 Date: Tue, 27 Jun 2006 14:46:05 EDT From: RSRICHMOND@aol.com Subject: [Histonet] who grosses and embeds? To: histonet@lists.utsouthwestern.edu Message-ID: <4ff.1ccebd8.31d2d6ed@aol.com> Content-Type: text/plain; charset="US-ASCII" The question of who is allowed to gross what surgical pathology specimens seems to keep coming up. Right now the regulatory agencies seem to be leaning toward making the pathologist do it all. In contrast, nobody seems to be interested in the embedder - who in effect re-grosses small biopsy specimens. When I was a resident (around 1970) we used to fill out a sheet - listing the number of biopsy specimens for example - that the embedder consulted while embedding. I've worked in maybe forty surgical pathology services since then (as a locum tenens pathologist, mostly) and I don't think I've ever seen an embedding sheet again. It's quite disconcerting when the endoscopist describes three specimens, I receive two, and the next day there's only one piece of tissue on the slide. It seems to me that the College of American Pathologists (CAP) should make it a Phase II (immediate remediation required) to embed small specimens without an embedding sheet. Cowpath, Tantrum, and other surgical pathology data bases should be able to generate a compact case list that the count of specimens could be handwritten onto - Cowpath at least requires that a compact specimen log be handwritten. And pathology residents should be required to come in during the wee hours and learn how to embed! Bob Richmond Knoxville TN and Gastonia NC ------------------------------ Message: 5 Date: Tue, 27 Jun 2006 14:54:27 -0400 From: "Weems, Joyce" Subject: RE: [Histonet] who grosses and embeds? To: , Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202960104@sjhaexc02.sjha.org> Content-Type: text/plain; charset="utf-8" Well said. We would pass on that requirement as we do still count pieces - written on the requisition for the transcriptionist to put in the gross template, and transferred to the Triple G system for the embedders. If we don't have the correct number of pieces, it is brought to the attention of the pathologist. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of RSRICHMOND@aol.com Sent: Tuesday, June 27, 2006 2:46 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] who grosses and embeds? The question of who is allowed to gross what surgical pathology specimens seems to keep coming up. Right now the regulatory agencies seem to be leaning toward making the pathologist do it all. In contrast, nobody seems to be interested in the embedder - who in effect re-grosses small biopsy specimens. When I was a resident (around 1970) we used to fill out a sheet - listing the number of biopsy specimens for example - that the embedder consulted while embedding. I've worked in maybe forty surgical pathology services since then (as a locum tenens pathologist, mostly) and I don't think I've ever seen an embedding sheet again. It's quite disconcerting when the endoscopist describes three specimens, I receive two, and the next day there's only one piece of tissue on the slide. It seems to me that the College of American Pathologists (CAP) should make it a Phase II (immediate remediation required) to embed small specimens without an embedding sheet. Cowpath, Tantrum, and other surgical pathology data bases should be able to generate a compact case list that the count of specimens could be handwritten onto - Cowpath at least requires that a compact specimen log be handwritten. And pathology residents should be required to come in during the wee hours and learn how to embed! Bob Richmond Knoxville TN and Gastonia NC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. ------------------------------ Message: 6 Date: Tue, 27 Jun 2006 15:09:48 -0400 From: bjdewe@aol.com Subject: [Histonet] IHC Workshop in California To: Histonet@lists.utsouthwestern.edu, MICROSCOPY@LISTSERV.MED.HARVARD.EDU Message-ID: <8C868346502B3F2-1BB0-DB7@mblk-d36.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" We still have a few spots left for our IHC on Animal Tissue workshop at UC Davis on July 28th. Here is a link to our flyer. http://www.vetmed.ucdavis.edu/vsr/dil/events.html To register go to: www.innvx.com and click on workshop on the home page... If you have any questions please ask;-) Cheers, Loralei Dewe Live as if you were to die tomorrow. Learn as if you were to live forever. - Gandhi - ******************************************************************* Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s)and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ******************************************************************* ________________________________________________________________________ Check out AOL.com today. Breaking news, video search, pictures, email and IM. All on demand. Always Free. ------------------------------ Message: 7 Date: Tue, 27 Jun 2006 12:51:41 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] who grosses and embeds? To: RSRICHMOND@aol.com, histonet@lists.utsouthwestern.edu Message-ID: <20060627195141.6616.qmail@web61225.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 The number of biopsy pieces in a log form to be consulted by the embedding HT is standard proceure at Mount Sinai Medical Center (MIami Beach), Ohio State University and Quest Diagnostics (South Florida). Ren? J. RSRICHMOND@aol.com wrote: The question of who is allowed to gross what surgical pathology specimens seems to keep coming up. Right now the regulatory agencies seem to be leaning toward making the pathologist do it all. In contrast, nobody seems to be interested in the embedder - who in effect re-grosses small biopsy specimens. When I was a resident (around 1970) we used to fill out a sheet - listing the number of biopsy specimens for example - that the embedder consulted while embedding. I've worked in maybe forty surgical pathology services since then (as a locum tenens pathologist, mostly) and I don't think I've ever seen an embedding sheet again. It's quite disconcerting when the endoscopist describes three specimens, I receive two, and the next day there's only one piece of tissue on the slide. It seems to me that the College of American Pathologists (CAP) should make it a Phase II (immediate remediation required) to embed small specimens without an embedding sheet. Cowpath, Tantrum, and other surgical pathology data bases should be able to generate a compact case list that the count of specimens could be handwritten onto - Cowpath at least requires that a compact specimen log be handwritten. And pathology residents should be required to come in during the wee hours and learn how to embed! Bob Richmond Knoxville TN and Gastonia NC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. ------------------------------ Message: 8 Date: Tue, 27 Jun 2006 17:55:25 -0400 From: "Osborn, Sharon" Subject: [Histonet] RE: Air purifier To: , Message-ID: <9A919A5D70313A4D9C56A025710874080C731D@kenmsg40.us.schp.com> Content-Type: text/plain; charset="us-ascii" Tom, For over two years now, we have had "R2D2" sitting on the floor of our lab so it can circulate the air while removing the wonderful perfumes that histology is well known for. The comments/complaints have decreased so much that other departments are clamoring for our $1500.00 solution. Additional filters are $449 each. We got this through ml-MarketLab JL9390 for the Air Filtration System with Filter. Replacement Filter is JL9266. www.MarketLabInc.com or 1.800.237.3604. Now, we very rarely have a complaint from someone about the odors; so that says it is working well. We leave it on 24/7 at the medium setting. The high setting stirs up too much dust for me allergies. sharon osborn DNAX, SP BioPharma Palo Alto, CA Date: Tue, 27 Jun 2006 09:44:44 -0400 From: "HSRL" Subject: [Histonet] Air Purifier for Xylene To: Message-ID: <001201c699ef$dc457210$0500a8c0@HSRLMAIN> Content-Type: text/plain; charset="us-ascii" Dear Netters, Has anyone used an air purifier made specifically for formaldehyde and xylene fumes? I know Newcomer Supply has started distributing the system. Does anyone have any feedback on the Newcomer system or any other air purifying system? Thanks for your feedback, Tom Galati Laboratory Director HSRL, Inc.- A GLP Compliant Contract Laboratory 5930 Main Street Mount Jackson, Virginia 22842 540.477.4440 Fax: 540.477.4448 tomgalati@hsrl.org www.hsrl.org ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. ------------------------------ Message: 9 Date: Wed, 28 Jun 2006 09:30:26 +0100 From: Kemlo Rogerson Subject: RE: [Histonet] who grosses and embeds? To: "'Rene J Buesa'" , RSRICHMOND@aol.com, histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain IMHO those who gross and embed should be those with the competency and training to do it; it could be a Pathologists, he/she could delegate to a SR or 'Junior Medic' that they have trained or it could be delegated to a BMS (Histotech) who is qualified (Specialist Exam) and competent. I have personally grossed skin biopsies, uterus, dead foals and budgies. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 People are like stained glass windows: they sparkle and shine when the sun is out, but when the darkness sets in their true beauty is revealed only if there is a light within. --Elizabeth Kubler-Ross This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation ------------------------------ Message: 10 Date: Wed, 28 Jun 2006 05:47:46 -0500 From: "Joe Nocito" Subject: Re: [Histonet] who grosses and embeds? To: , Message-ID: <006001c69aa0$4b1d15c0$0b69ce44@yourxhtr8hvc4p> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Dr. R, you bring up an excellent point. We have one pathologist that refuses to cut shave bxs, vas deferens, fallopian tubes, temporal arteries and other small specimens. I have instructed my embedders that they have to cut these specimens for proper orientation. Is this considered grossing? As far as grossing sheets, every lab that I have worked in, plus the lab that I started, always had a grossing sheet. It's almost mal-practice not to. How could you tell if a specimen has special instructions or not such as a renal bx? Just my 3 cents worth. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: To: Sent: Tuesday, June 27, 2006 1:46 PM Subject: [Histonet] who grosses and embeds? > The question of who is allowed to gross what surgical pathology specimens > seems to keep coming up. Right now the regulatory agencies seem to be > leaning > toward making the pathologist do it all. > > In contrast, nobody seems to be interested in the embedder - who in effect > re-grosses small biopsy specimens. When I was a resident (around 1970) we > used > to fill out a sheet - listing the number of biopsy specimens for example - > that > the embedder consulted while embedding. I've worked in maybe forty > surgical > pathology services since then (as a locum tenens pathologist, mostly) and > I > don't think I've ever seen an embedding sheet again. > > It's quite disconcerting when the endoscopist describes three specimens, I > receive two, and the next day there's only one piece of tissue on the > slide. > > It seems to me that the College of American Pathologists (CAP) should make > it > a Phase II (immediate remediation required) to embed small specimens > without > an embedding sheet. > > Cowpath, Tantrum, and other surgical pathology data bases should be able > to > generate a compact case list that the count of specimens could be > handwritten > onto - Cowpath at least requires that a compact specimen log be > handwritten. > > And pathology residents should be required to come in during the wee hours > and learn how to embed! > > Bob Richmond > Knoxville TN and Gastonia NC > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.394 / Virus Database: 268.9.5/376 - Release Date: 6/26/2006 > > ------------------------------ Message: 11 Date: Wed, 28 Jun 2006 12:19:08 +0100 From: "Marshall Terry Dr, Consultant Histopathologist" Subject: RE: [Histonet] who grosses and embeds? To: "Kemlo Rogerson" , "Rene J Buesa" , , Message-ID: Content-Type: text/plain; charset="iso-8859-1" So, you got a feather in your cap after flogging a dead horse. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:kemlo.rogerson@waht.swest.nhs.uk] Sent: 28 June 2006 09:30 To: 'Rene J Buesa'; RSRICHMOND@aol.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] who grosses and embeds? IMHO those who gross and embed should be those with the competency and training to do it; it could be a Pathologists, he/she could delegate to a SR or 'Junior Medic' that they have trained or it could be delegated to a BMS (Histotech) who is qualified (Specialist Exam) and competent. I have personally grossed skin biopsies, uterus, dead foals and budgies. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 People are like stained glass windows: they sparkle and shine when the sun is out, but when the darkness sets in their true beauty is revealed only if there is a light within. --Elizabeth Kubler-Ross This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Wed, 28 Jun 2006 12:49:22 +0000 From: Einar J?rundsson Subject: [Histonet] CD68 in FFPE mouse tissue To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" I am having problems with IHC staining with macrophage-markers in formalin-fixed paraffin-embedded (FFPE) mouse arotic root sections. F4/80 (Serotec) works well in spleen (after proteinase K digestion) but is inconsistent in aortas. I have also tried CD68 clone FA11 (Serotec) with proteinase K digestion and HIER (citrate, borate, Tris-HCl) with no success. Any tips? Regards, Einar. ------------------------------ Message: 13 Date: Wed, 28 Jun 2006 14:00:28 +0100 From: Subject: [Histonet] Anti Glycine Transporter 1 To: Message-ID: <90B6684A9D6DAF468F7A5DC148754E1D2154D4@ALPW31.f2.enterprise> Content-Type: text/plain; charset="iso-8859-1" Hi Does anyone have any experience/Protocols for ICC staining with Anti-Glycine Transporter 1 on rat Brain/Liver This is a new one for me and have been asked if I can try to get a method working pretty quickly, so if anyone has any experience they could share I would be really grateful. Thanks Susan ------------------------------ Message: 14 Date: Wed, 28 Jun 2006 08:34:03 -0500 From: "Lester Raff" Subject: [Histonet] Dako Autostainer To: Message-ID: <5DA1CA5D0B98A84985B545A24423B822A501@UPLAB01.uplab.local> Content-Type: text/plain; charset="iso-8859-1" Hello, and thanks to all for their input on the PASCAL pressure cooker. Another IHC question. We use the Dako Autostainer Plus and occassionaly have areas on the slides that don't seem to stain. We are careful not to let the slides dry out. Do Autostainers have a problem distributing reagents evenly? Thanks, Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory ph: 708-486-0076 fax: 708-486-0080 ------------------------------ Message: 15 Date: Wed, 28 Jun 2006 06:48:52 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] Dako Autostainer To: Lester Raff , histonet@lists.utsouthwestern.edu Message-ID: <20060628134852.21343.qmail@web61214.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 They should not but if you are diluting concentrated Abs you should remember to add Tween 20 to assure even distribution. Ren? J. Lester Raff wrote: Hello, and thanks to all for their input on the PASCAL pressure cooker. Another IHC question. We use the Dako Autostainer Plus and occassionaly have areas on the slides that don't seem to stain. We are careful not to let the slides dry out. Do Autostainers have a problem distributing reagents evenly? Thanks, Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory ph: 708-486-0076 fax: 708-486-0080 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- How low will we go? Check out Yahoo! Messenger's low PC-to-Phone call rates. ------------------------------ Message: 16 Date: Wed, 28 Jun 2006 09:43:06 -0500 From: "Webb, Dorothy L" Subject: [Histonet] Davidson's To: Histonet@lists.utsouthwestern.edu Message-ID: <0E394B648E5284478A6CCB78E5AFDA270171FE69@hpes1.HealthPartners.int> Content-Type: text/plain; charset="us-ascii" Has anyone had any problems with Davidson's fixative interfering with their IHC staining? We have not run any comparison studies, but, one of our pathologists heard "whispers" of this amongst fellow pathologists at another facility!! Any thoughts on this would be appreciated!! ------------------------------ Message: 17 Date: Wed, 28 Jun 2006 10:38:22 -0500 From: "Henry, Charlene" Subject: RE: [Histonet] Any suggestion on Promega or Chemicon TUNEL System onhuman tissue.. . To: "soofia siddiqui" , Message-ID: <5CB39BCA5724F349BCB748675C6CA1A2099A1CFE@SJMEMXMB02.stjude.sjcrh.local> Content-Type: text/plain; charset="US-ASCII" We use the Promega Dead End kit and it works great; however when we do a TUNEL assay, we also run pHistone IHC. Charlene Henry HT (ASCP), QIHC Histology/Immunohistochemistry Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of soofia siddiqui Sent: Tuesday, June 27, 2006 12:55 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Any suggestion on Promega or Chemicon TUNEL System onhuman tissue.. . I am going to start Staining on human skin Using TUNEL System. I wanted to get some information about the vender if someone have succesfully used any system on human tissue.I am looking at Promega and Chemicon but really want to get some feed back if someone have used any of them or some other . ss --------------------------------- Yahoo! Sports Fantasy Football '06 - Go with the leader. Start your league today! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Wed, 28 Jun 2006 11:46:07 EDT From: RSRICHMOND@aol.com Subject: Re: [Histonet] who grosses and embeds? To: histonet@lists.utsouthwestern.edu Message-ID: <53c.19c47aa.31d3fe3f@aol.com> Content-Type: text/plain; charset="US-ASCII" Kemlo Rogerson notes: >>I have personally grossed skin biopsies, uterus, dead foals and budgies.<< Presumably not John Lennon's budgie - I have a little budgie he is my very pal I take him walks in Britain I hope I always shall. Bob Richmond ------------------------------ Message: 19 Date: Wed, 28 Jun 2006 12:04:27 -0400 From: "Ron Martin" Subject: [Histonet] need safety manual info To: Message-ID: <000401c69acc$88789e90$2ea2ac45@D6WRV2B1> Content-Type: text/plain; charset="US-ASCII" If anyone is willing to share some info about their safety manual, I would appreciate it. I need info on: chemical hygiene plans, ergonomics and OSHA regulations. Thanks in advance Ron Martin ------------------------------ Message: 20 Date: Wed, 28 Jun 2006 12:33:32 -0400 From: "Tom McNemar" Subject: RE: [Histonet] Dako Autostainer To: "Lester Raff" , Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F3D8@lmhsmail.lmhealth.org> Content-Type: text/plain; charset="iso-8859-1" What counterstain do you use? Hematoxylin will clumb and clog the probe causing uneven staining. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Lester Raff Sent: Wednesday, June 28, 2006 9:34 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Dako Autostainer Hello, and thanks to all for their input on the PASCAL pressure cooker. Another IHC question. We use the Dako Autostainer Plus and occassionaly have areas on the slides that don't seem to stain. We are careful not to let the slides dry out. Do Autostainers have a problem distributing reagents evenly? Thanks, Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory ph: 708-486-0076 fax: 708-486-0080 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Wed, 28 Jun 2006 10:22:18 -0600 From: themagoos@rushmore.com Subject: [Histonet] TBS Microwave Programs To: histonet@lists.utsouthwestern.edu Message-ID: <1151511738.44a2acba352de@webmail.rushmore.com> Content-Type: text/plain; charset=ISO-8859-1 We have a TBS Shurwave 1200 and we are trying to process fatty skin specimens. Does anybody have a sample program for microwave processing fatty skin specimens? We are also looking at microwave processing all types of specimens. Any feedback would be appreciated. Thank you. Jason McGough HT(ASCP) Clinical LAboratory of the Black Hills 2805 5th Street Rapid City, SD 57701 605-343-2267 ------------------------------------------------- This mail sent by http://webmail.rushmore.com ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 31, Issue 40 **************************************** Confidential: This electronic message and all contents contain information from BayCare Health System which may be privileged, confidential or otherwise protected from disclosure. The information is intended to be for the addressee only. If you are not the addressee, any disclosure, copy, distribution or use of the contents of this message is prohibited. If you have received this electronic message in error, please notify the sender and destroy the original message and all copies. From liz <@t> premierlab.com Wed Jun 28 13:34:51 2006 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Jun 28 13:35:02 2006 Subject: [Histonet] CD68 in FFPE mouse tissue In-Reply-To: Message-ID: <000e01c69ae1$8b672670$0300a8c0@Chlipala> Einar We use the same antibodies on mouse aortic root sections and I have never had a problem with either of them. We perform the immunostains on frozen sections. We follow Gayles protocol for sectioning and fixation, air dry overnight and then fix in a mixture of acetone/ethanol and then proced with staining. The stains always turn out great. My protocol might be a bit different I use dako's rabbit anti-rat for my secondary and then I use their rabbit envision HRP. I can send you some images if you want to see how they work for us. I have not tried the CD68 on paraffin sections yet but the F4/80 works great on 10% NBF fixed paraffin sections Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Einar J?rundsson Sent: Wednesday, June 28, 2006 6:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD68 in FFPE mouse tissue I am having problems with IHC staining with macrophage-markers in formalin-fixed paraffin-embedded (FFPE) mouse arotic root sections. F4/80 (Serotec) works well in spleen (after proteinase K digestion) but is inconsistent in aortas. I have also tried CD68 clone FA11 (Serotec) with proteinase K digestion and HIER (citrate, borate, Tris-HCl) with no success. Any tips? Regards, Einar. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1.1631 (20060628) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From ynwang <@t> u.washington.edu Wed Jun 28 13:43:57 2006 From: ynwang <@t> u.washington.edu (Yak-Nam Wang) Date: Wed Jun 28 13:43:18 2006 Subject: [Histonet] Change in tissue preparation- paraffin to OCT Message-ID: Hello, We have various tissues fixed in formalin and brought to 70% alcohol (as if we were going to embedd in paraffin). However, due to limited equipment access (we now have access to a cryostat but not a microtome), we would like to prep this tissue for cryosections. Seeing as it is at 70% alcohol, would I need to bring it to 100% PBS before running it through the sucrose solutions to cryoprotect? Also, does anyone know if there would be any bad tissue effects of us doing this - i.e. Should we try harder to find a microtome? Thanks in advance for your help Yak-Nam Wang From holliday.casey <@t> gmail.com Wed Jun 28 15:23:15 2006 From: holliday.casey <@t> gmail.com (Casey Holliday) Date: Wed Jun 28 15:23:20 2006 Subject: [Histonet] Staining MMA flourochrome labeled sections Message-ID: Hello all, I have a modest number of bone-labeled specimens about ready to be "fixed" and embedded in MMA. To make the most out of the data set, I was hoping to stain (Toluidine Blue) the same sections for tissue morphology that I was going to use for studying bone deposition. Are there any problems, hinderances, or special techniques with using T-Blue (or another preferential stain) on these tetracycline/calcein labeled slides? Second, is alcoholic formalin now considered to be superior to alcohol for retaining the tissue's morphology in plastic embedding/sectioning. Many thanks Casey -- Casey Holliday, Ph.D. Instructor of Anatomical Sciences Postdoctoral Researcher Ohio University College of Osteopathic Medicine Dept. Biomedical Sciences Ohio University From gcallis <@t> montana.edu Wed Jun 28 15:50:26 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Jun 28 15:50:36 2006 Subject: [Histonet] Gr-1 staining in mouse spleen In-Reply-To: <21E81F01D3A58943AD79F8753AEF65A24A7442@M-EXVMB2.hlm.ad.mof fitt.usf.edu> References: <21E81F01D3A58943AD79F8753AEF65A24A7442@M-EXVMB2.hlm.ad.moffitt.usf.edu> Message-ID: <6.0.0.22.1.20060628142227.01b305e8@gemini.msu.montana.edu> GR-1 i.e. Ly-G6 Clone RB6-8CS should work very well with frozen sections, and it should stain in 30 minutes incubation time. In fact, the CD4 and CD8 should stain in the same way with the same timing as suggested below. However, endogenous peroxidase blocking using methanol is known to be very bad for lymphocyte CD type markers, I would use DAKO's peroxidase block in a buffer for frozen sections. You need to do a dilution panel for the primary antibody, starting with a target concentration of 10 ug/ml and dilute out. Your antibody is far to concentrated is you are using a direct application of 0.5mg/ml!!! The recommended concentration for this antibody (found in specification sheet from BD) is 1 to 5 ug/ml, which is a 1:500 dilution of the 0.5mg/ml!!!! So please note the comments in your protocol below. At 11:54 AM 6/28/2006, you wrote: >I have gotten wonderfully accurate information from histonet in the >past! Now, I have been trying for Gr-1 staining of mouse spleen for >weeks. The best result have come only by direct application of the BD >antibody at 0.5mg/mL and the results look like all of the white pulp cells >are positive. Naive mice and tumor bearing mice look identical. There is >nothing on my "no primary" controls. However, I doubt this staining is >specific. My protocol is as follows: > > >* Thaw slide mounted frozen sections (in foil pack), air dry, and >fix in acetone 10 minutes at RT. Frozen sections should be totally dry, preferrably overnight, acetone fixation is fine 10 min at 4C, then air dry, go to buffer rinses. Try 75% acetone/25% absolute ethanol for 5 min at RT, but go directly to buffer for 3 changes - you may find the morphology is better and you have excellent staining of the granulocytes. >* Air dry and apply PAP pen >* Hydrate in PBS (and removes embedding medium) the rest of the >protocol at RT >* Block endogenous peroxidase with 1:1:8 methanol:30% H2O2:PBS for >15 min. Don't use methanol peroxidase blocking for murine CD markers, use the DAKO peroxidase block for FS, 0.03% hydrogen peroxide or bring down the concentration of your hydrogen peroxide to 0.1% or even less diluted in buffer for 10 min >* Wash 3x PBS 5 min >* Permeabilize 30 min with 1.8% lysine. 0.2% triton X-100, 4% serum, >in PBS You do not need to permeabilize, these are cell surface markers, and simply do a normal serum block of 10% serum matched to the host of the secondary antibody (which should be adsorbed to mouse). Triton X-100 can remain but it should be used in all buffers, diluents, and you may want to reduce the concentration with a minimally fixed frozen section. We add 2.5% mouse serum to our NSB when doing most of the rat antimouse CD markers. That concentration can vary from 1 to 5% and also use this NSB to dilute your secondary antibody. >* Quick rinse in PBS >* Direct application of antibody BD# 553123 incubate overnight. NO, this antibody needs to optimized with that dilution panel, and read the BD data sheet for recommended concentration, dilute the antibody in 5% normal serum matched to host of secondary antibody, you can use less for 30 min. >* Wash 3x PBS 5 min >* Vector biotinylated secondary #BA4001 rabbit anti-rat, 1:1000 >dilution, 2 hours Secondary antibody should have a concentration of 2 - 5 ug/ml, dilute in the normal serum block and incubate for 30 min at RT We prefer to use goat or donkey antiRat (F(Ab')2 frag of IgG (0.5mg/ml) diluted 1:250 for 30 min at RT. >* Wash 3x PBS 5 min >* Vectastain ABC kit PK-4000 one hour I presume this is the Elite kit, and you follow kit directions - 30 min is the recommended time?? at RT. >* Wash 2x PBS, 1x TBS, 5 min Instead of having to change buffers use TBS instead of PBS throughout, much easier. >* DAB 0.05% + nickelous ammonium sulfate 0.5% in TBS 5 min >* Add 0.1% of 30% H2O2 for 5 min to develop black color Watch your DAB develop using a microscope. >* wash, dehydrate, coverslip with DPX > >I have tried the Serotec product as well as the BD IHC product for Gr-1, >and various dilutions of primary to no avail. Note that I get excellent >T-cell staining with CD4 and CD8a with this same protocol, with similar >distribution to the white pulp. I have not found many examples of Gr-1 >IHC in the literature. Has anyone performed this staining routinely and >what type of distribution do you see? Any comments or suggestions would >be greatly appreciated. There is a picture of GR-1 on the BD website or should be. You can contact their tech services about this also. You said nothing about your negative control, and it should be Rat IgG 2b isotype at the same concentration. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From crains <@t> wpmpath.com Wed Jun 28 17:05:41 2006 From: crains <@t> wpmpath.com (crains@wpmpath.com) Date: Wed Jun 28 17:05:51 2006 Subject: [Histonet] Histotech Job Opening Message-ID: <20060628220542.VDQL3326.dukecmmtao03.coxmail.com@dukecmmtao03> Have an opening for an experienced Histotech. Established regional independent Path/Cyto lab with 5 Pathologists. Busy, but laid back work environment. Pay is competitive and benefits are very good. Located in city of 45,000 with healthy, progressive medical community. Ventana immuno. experience/knowledge is a plus, but not required. Contact me for additional information. crains@wpmpath.com Chris Rains WPM Pathology Laboratory Salina, KS Important: This email and any attachments may contain confidential information subject to protection under the Federal Standards for Privacy of Individually Identifiable Health Information (45 C.F.R. Parts 160 and 164). If you or your organization is a "Covered Entity" under the above mentioned regulations, you are obligated to treat such information in a manner consistent with the regulations. If it appears that this email was sent to you in error, (1) you are prohibited from utilizing or disseminating this email or any attachments; (2) please immediately delete it from your computer and any servers or other locations where it might be stored and email (crains@wpmpath.com) or call (Chris Rains) at 785-823-7201 advising that you have done so. We appreciate your cooperation. From kemlo.rogerson <@t> waht.swest.nhs.uk Thu Jun 29 01:50:00 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Jun 29 01:49:19 2006 Subject: [Histonet] need safety manual info Message-ID: Would like to but mine's written in English, sorry! Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 People are like stained glass windows: they sparkle and shine when the sun is out, but when the darkness sets in their true beauty is revealed only if there is a light within. --Elizabeth Kubler-Ross This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From cmalc <@t> unimelb.edu.au Thu Jun 29 01:56:51 2006 From: cmalc <@t> unimelb.edu.au (Cathy Malcontenti-Wilson) Date: Thu Jun 29 01:57:07 2006 Subject: [Histonet] CD11c antibody that works on IHC on paraffin sections Message-ID: <6.2.1.2.2.20060629165436.02c94360@mail.staff.unimelb.edu.au> Dear All, Does anyone know of a CD11c antibody for mouse tissue that works in paraffin sections for IHC? Thanks in advance! Cathy Malcontenti-Wilson From Andrew.Prior <@t> Smith-Nephew.com Thu Jun 29 02:18:24 2006 From: Andrew.Prior <@t> Smith-Nephew.com (Prior, Andrew) Date: Thu Jun 29 02:24:12 2006 Subject: [Histonet] lipofuscin detection Message-ID: Have you tried using fluorescence? Lipofuscin fluoresces under green light, can't remember what the emission is, I think it is red. Do a search for papers by Dr Matt Sheehy, of Leicester University, UK who works on Cancer pagurus. Hope this helps Andrew Andrew Prior Histologist Smith &Nephew Research Centre York Science Park Heslington York YO10 5DF UK Andrew.Prior@smith-nephew.com 01904 824022 Confidentiality. This electronic transmission is strictly confidential to Smith & Nephew and intended solely for the addressee. It may contain information which is covered by legal, professional or other privilege. If you are not the intended addressee, or someone authorised by the intended addressee to receive transmissions on behalf of the addressee, you must not retain, disclose in any form, copy or take any action in reliance on this transmission. If you have received this transmission in error, please notify the sender as soon as possible and destroy this message. From David.Muskett <@t> RLC.NHS.UK Thu Jun 29 02:37:40 2006 From: David.Muskett <@t> RLC.NHS.UK (Muskett David) Date: Thu Jun 29 02:37:45 2006 Subject: [Histonet] Some questions about mucins Message-ID: Dear All I have been looking through some books and papers prior to giving a tutorial to my colleagues about carbohydrates and mucins. 2 questions Leucodye I seem to recall in the dim and distant past this term was used for Schiff's reagent. Is this term still applicable? Alcian Blue mechanism I am aware that the binding of alcian blue and acid mucins is permanent but does anyone know the exact mechanism? Thanks in advance David David Muskett Chief Biomedical Scientist, Histology Royal Liverpool Children's NHS Trust Eaton Road Liverpool L12 2AP Internal telephone x3615 Telephone (0151) 293 3656 Fax (0151) 293 3617 http://nhsald02/histopathology From kemlo.rogerson <@t> waht.swest.nhs.uk Thu Jun 29 02:39:20 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Jun 29 02:38:37 2006 Subject: [Histonet] lipofuscin detection Message-ID: It fluoresces brown under ultraviolet light but as with the melanins, there are many lipofuchsins too. It (lipofuchsin) can be extracted by acetone or staining prevented by methylation with 0.1N HCL in methanol for 4 to 6 hrs at 60 degrees C (saponification restores staining, apparently). Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 People are like stained glass windows: they sparkle and shine when the sun is out, but when the darkness sets in their true beauty is revealed only if there is a light within. --Elizabeth Kubler-Ross This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From kemlo.rogerson <@t> waht.swest.nhs.uk Thu Jun 29 03:05:00 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Jun 29 03:04:19 2006 Subject: [Histonet] Some questions about mucins Message-ID: Alician blues are a family of polyvalent basic dyes with at least two and as many as four isothiouronium groups per phthalocyanin ring. It works via salt linkage with polyanions (heparain, DNA and hyaluronate) to give insoluble precipitates. (Lillie) Increasing concentrations of electrolytes in the staining solution results in increased combination of Alcian blue molecules. So it reacts by salt linkages and can be enhanced by increasing electrolyte concentration (NaCl, KCl, LiBr, mgCl, etc) Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 People are like stained glass windows: they sparkle and shine when the sun is out, but when the darkness sets in their true beauty is revealed only if there is a light within. --Elizabeth Kubler-Ross This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From daniel.eberhard <@t> uni-bielefeld.de Thu Jun 29 03:41:10 2006 From: daniel.eberhard <@t> uni-bielefeld.de (Daniel Eberhard) Date: Thu Jun 29 03:41:28 2006 Subject: [Histonet] Hes1 antibody Message-ID: <44A39226.1080003@uni-bielefeld.de> Dear All, we are looking for a reliable Hes1 antibody to stain mouse/human cells&tissue. Any recommendations? Thank you Daniel. From DGNajarian <@t> comcast.net Thu Jun 29 05:27:01 2006 From: DGNajarian <@t> comcast.net (Dennis Najarian) Date: Thu Jun 29 05:27:10 2006 Subject: [Histonet] CD11c antibody that works on IHC on paraffin sections In-Reply-To: <6.2.1.2.2.20060629165436.02c94360@mail.staff.unimelb.edu.au> Message-ID: Hi Cathy, Try Chemicon: http://www.chemicon.com/browse/productdetail.asp?ProductID=MAB1399Z Good luck! Dennis -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cathy Malcontenti-Wilson Sent: Thursday, June 29, 2006 2:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CD11c antibody that works on IHC on paraffin sections Dear All, Does anyone know of a CD11c antibody for mouse tissue that works in paraffin sections for IHC? Thanks in advance! Cathy Malcontenti-Wilson _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgirlnow <@t> msn.com Thu Jun 29 07:53:09 2006 From: godsgirlnow <@t> msn.com (Roxanne Soto) Date: Thu Jun 29 07:53:19 2006 Subject: [Histonet] INKING Message-ID: I have a grossing question..... When I gross in Colon Bxs, I ink the stalk and have always used bouins, or vinegar to mordant the ink. I have been told that I shouldn't do this because it affects the tissue down the line, has anyone else experienced this? Roxanne Tampa From Terry.Marshall <@t> rothgen.nhs.uk Thu Jun 29 08:11:25 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Thu Jun 29 08:08:28 2006 Subject: [Histonet] INKING Message-ID: I find it hard to believe that the usual waft in some alleged mordanting fluid has any effect on anything. PS is the opposite of inking, outking or inqueen? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Roxanne Soto [mailto:godsgirlnow@msn.com] Sent: 29 June 2006 13:53 To: HISTONET@PATHOLOGY.SWMED.EDU Subject: [Histonet] INKING I have a grossing question..... When I gross in Colon Bxs, I ink the stalk and have always used bouins, or vinegar to mordant the ink. I have been told that I shouldn't do this because it affects the tissue down the line, has anyone else experienced this? Roxanne Tampa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Thu Jun 29 08:09:38 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Jun 29 08:09:46 2006 Subject: [Histonet] Some questions about mucins Message-ID: <09C945920A6B654199F7A58A1D7D1FDE0171774B@lsexch.lsmaster.lifespan.org> "Leuco dyes" is a general term for any colorless compounds resulting from hydrogen reduction of a colored dye molecule. The term still has its place in dye chemistry, though I seldom hear it used in histological circles. The term "leucofuchsin" more specifically describes Schiff Reagent, since this leuco dye results from hydrogen reduction of fuchsin, which is the chromogen in Schiff Reagent. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > Muskett David > Sent: Thursday, June 29, 2006 12:37 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Some questions about mucins > > <> > Dear All > > I have been looking through some books and papers prior to giving a > tutorial to my colleagues about carbohydrates and mucins. 2 questions > > Leucodye > > I seem to recall in the dim and distant past this term was used for > Schiff's reagent. Is this term still applicable? > > Alcian Blue mechanism > I am aware that the binding of alcian blue and acid mucins is permanent > but does anyone know the exact mechanism? > > Thanks in advance > > David > > David Muskett > Chief Biomedical Scientist, Histology > Royal Liverpool Children's NHS Trust > Eaton Road > Liverpool > L12 2AP > > Internal telephone x3615 > Telephone (0151) 293 3656 > Fax (0151) 293 3617 > http://nhsald02/histopathology > > > > From kemlo.rogerson <@t> waht.swest.nhs.uk Thu Jun 29 08:16:26 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Jun 29 08:15:45 2006 Subject: [Histonet] INKING Message-ID: Geez; the opposite of 'inking' is 'no inking', thought you were bright! Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Distance is meant to relate, not separate. --Satish Kumar This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Terry.Marshall <@t> rothgen.nhs.uk Thu Jun 29 08:23:32 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Thu Jun 29 08:20:32 2006 Subject: [Histonet] INKING Message-ID: Thanks Kemlo, I had no inkling of the correct answer. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:kemlo.rogerson@waht.swest.nhs.uk] Sent: 29 June 2006 14:16 To: Marshall Terry Dr, Consultant Histopathologist; Roxanne Soto; HISTONET@PATHOLOGY.SWMED.EDU Subject: RE: [Histonet] INKING Geez; the opposite of 'inking' is 'no inking', thought you were bright! Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Distance is meant to relate, not separate. --Satish Kumar This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From ROrr <@t> enh.org Thu Jun 29 08:24:00 2006 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Thu Jun 29 08:24:09 2006 Subject: [Histonet] T. gondii Message-ID: Hello everyone, I'm in need of T. gondii control tissue. Any one have it? I'll be glad to make a trade and be forever in your debt. Thank-you, Becky Becky Orr CLA,HT(ASCP) Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 From cpomajzl <@t> cpllabs.com Thu Jun 29 08:34:56 2006 From: cpomajzl <@t> cpllabs.com (Chris Pomajzl) Date: Thu Jun 29 08:30:22 2006 Subject: [Histonet] INKING References: Message-ID: <006601c69b80$cfdc5380$26fca8c0@CSP> TRIVIA CHALLENGE OF THE DAY: Name one eight letter word that has kst in the middle, in the beginning, and at the end. ----- Original Message ----- From: "Kemlo Rogerson" To: "'Marshall Terry Dr, Consultant Histopathologist'" ; "Roxanne Soto" ; Sent: Thursday, June 29, 2006 8:16 AM Subject: RE: [Histonet] INKING > Geez; the opposite of 'inking' is 'no inking', thought you were bright! > > Kemlo Rogerson > Pathology Manager > Ext 3311 > DD 01934 647057 > Mob 07749 754194 > > Distance is meant to relate, not separate. --Satish Kumar > > > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on its > contents: to do so is strictly prohibited and may be unlawful. Please inform > me that this message has gone astray before deleting it. Thank you for your > co-operation > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Thu Jun 29 08:31:18 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Thu Jun 29 08:31:22 2006 Subject: AW: [Histonet] Some questions about mucins In-Reply-To: Message-ID: <001c01c69b80$4d9caaf0$eeeea8c0@SERVER01> The basic alcianblue binds to anionic charged sides of sulfated and carboxylated carbohydrates (acid mucins). This works at pH 2. At lower pH only sulfated carbohydrates are stained, because the anionic charge of the others is repressed. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Muskett David Gesendet: Donnerstag, 29. Juni 2006 09:38 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Some questions about mucins Dear All I have been looking through some books and papers prior to giving a tutorial to my colleagues about carbohydrates and mucins. 2 questions Leucodye I seem to recall in the dim and distant past this term was used for Schiff's reagent. Is this term still applicable? Alcian Blue mechanism I am aware that the binding of alcian blue and acid mucins is permanent but does anyone know the exact mechanism? Thanks in advance David David Muskett Chief Biomedical Scientist, Histology Royal Liverpool Children's NHS Trust Eaton Road Liverpool L12 2AP Internal telephone x3615 Telephone (0151) 293 3656 Fax (0151) 293 3617 http://nhsald02/histopathology From Jacqueline.Malam <@t> rli.mbht.nhs.uk Thu Jun 29 08:36:35 2006 From: Jacqueline.Malam <@t> rli.mbht.nhs.uk (Malam Jacqueline) Date: Thu Jun 29 08:38:32 2006 Subject: [Histonet] Re: Inking Message-ID: I thought it was aninking (or possibly Tippex) Jacqui DISCLAIMER: This e-mail is confidential and privileged. If you are not the intended recipient please accept our apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform postmaster@rli.mbht.nhs.uk that this message has gone astray before deleting it. Comments or opinions expressed in this email are those of their respective contributors only. The views expressed do not represent the views of the Trust, its management or employees. University Hospitals of Morecambe Bay NHS Trust is not responsible and disclaims any and all liability for the content of comments written within.Thank you for your co-operation. From Terry.Marshall <@t> rothgen.nhs.uk Thu Jun 29 08:59:14 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Thu Jun 29 08:56:12 2006 Subject: [Histonet] INKING Message-ID: inkstand Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Chris Pomajzl [mailto:cpomajzl@cpllabs.com] Sent: 29 June 2006 14:35 To: HISTONET Subject: Re: [Histonet] INKING TRIVIA CHALLENGE OF THE DAY: Name one eight letter word that has kst in the middle, in the beginning, and at the end. ----- Original Message ----- From: "Kemlo Rogerson" To: "'Marshall Terry Dr, Consultant Histopathologist'" ; "Roxanne Soto" ; Sent: Thursday, June 29, 2006 8:16 AM Subject: RE: [Histonet] INKING > Geez; the opposite of 'inking' is 'no inking', thought you were bright! > > Kemlo Rogerson > Pathology Manager > Ext 3311 > DD 01934 647057 > Mob 07749 754194 > > Distance is meant to relate, not separate. --Satish Kumar > > > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on its > contents: to do so is strictly prohibited and may be unlawful. Please inform > me that this message has gone astray before deleting it. Thank you for your > co-operation > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From fferreir <@t> ibmc.up.pt Thu Jun 29 09:46:25 2006 From: fferreir <@t> ibmc.up.pt (=?iso-8859-1?b?RuF0aW1h?= Ferreirinha) Date: Thu Jun 29 10:11:09 2006 Subject: [Histonet] Optimal thickness for cutting of IHC sections Message-ID: <1151592385.44a3e7c1bca98@webmail.ibmc.up.pt> Hi Histonetters, I'm interested to see innervation (skin, gut,..) by IHC and I would like to know the adequate section thickness for this work. Although in general people say that a 5-10um thickness is OK for IHC sections and that section thickness is not critical for IHC (because one is only staining the exposed surface of the tissue) I've noticed that lots of papers regarding this matter refer to very thick sections (50um)! Why is this so? Is there some particularity for this kind of analysis? Thank you, F?tima -- F?tima Ferreirinha IBMC R. do Campo Alegre, 823 4150-180, Porto Portugal ---------------------------------------------------------------- This message was sent using IMP, the Internet Messaging Program. From bill501 <@t> mindspring.com Thu Jun 29 10:21:09 2006 From: bill501 <@t> mindspring.com (Bill) Date: Thu Jun 29 10:21:30 2006 Subject: [Histonet] INKING In-Reply-To: References: Message-ID: At 2:23 PM +0100 6/29/06, Marshall Terry Dr, Consultant Histopathologist wrote: >Thanks Kemlo, I had no inkling of the correct answer. Nicely set up, Terry -- ______________ Bill Blank, MD Heartland Lab From jamie.erickson <@t> abbott.com Thu Jun 29 12:06:37 2006 From: jamie.erickson <@t> abbott.com (Jamie E Erickson) Date: Thu Jun 29 12:06:42 2006 Subject: [Histonet] Re:Gr-1 staining in mouse spleen (Herber, Donna L.) In-Reply-To: <54so0d$lh315@viper.abbott.com> Message-ID: HI Donna, I've had great results in mouse granulocytic tumors with Gr-1 but my protocol was very standard you can check out the protocol in this journal article. Kyriaki Dunussi-Joannopoulos, Jamie Erickson et al... Efficacious immunomodulatory activity of the chemokine stromal cell-derived factor 1 (SDF-1): local secretion of SDF-1 at the tumor site serves as T-cell chemoattractant and mediates T-cell-dependent antitumor responses. Blood 100(5): 1551-1558, 2002 Sept. I used frozen sections and the Gr-1 antibody from BD @ 2ug/ml (LY-6G), RB6-8C5 Catalog #: 550291 (FOR IHC) we used this to check that granulocytes in and around that tumor. I think you may be using the wrong antibody this one is stated to work in IHC by BD( CAT # 550291,RB68C5) and is the same clone I use. You might want to look into that. Jamie _______________________________ Jamie Erickson Sr. Research Associate Department: DSMP Abbott Bioresearch Center 100 Research Drive Worcester, MA 01605-4341 508-688-3134 FAX: 508-793-4895 e-mail: jamie.erickson@abbott.com From Reuel.Cornelia <@t> tsrh.org Thu Jun 29 13:11:09 2006 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Thu Jun 29 13:13:47 2006 Subject: [Histonet] Feedback on Powerpath/Computer Documentation Message-ID: Is it worth using Powerpath(Tamtron) if you do not have enough clinical cases but rather used it more for research accession. Is it worth the service maintenance fee($17,000) I paid per year for research accession and not for clinical cases which the software was built for. Do you have any suggestion of which other software that will meet our needs without paying too much with service maintenance lesser than we have or you only pay for the installation without maintenance fee(Just wonderin if there is anything like this). Your opinion is highly appreciated.Thank you. reuel TSRH dallas,TX ******************************************************************************************************************* Texas Scottish Rite Hospital for Children is one of the nation's leading pediatric centers for the treatment of orthopedic conditions and learning disorders, like dyslexia. This email transmission and/or its attachments may contain confidential health information, intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing it to any other party unless required to do so by law and is required to delete/destroy the information after its stated need has been fulfilled. If you are not the intended recipient, any disclosure, copying, distribution or action taken in reliance on the contents of this email transmission is prohibited. If you have received this information in error, please notify the sender immediately and delete this information. We appreciate your efforts to protect the children's confidential information. ******************************************************************************************************************* From john_ronan <@t> merck.com Thu Jun 29 13:46:50 2006 From: john_ronan <@t> merck.com (Ronan, John) Date: Thu Jun 29 13:47:21 2006 Subject: [Histonet] TRIVIA CHALLENGE OF THE DAY: Message-ID: TRIVIA CHALLENGE OF THE DAY: Name one eight letter word that has kst in the middle, in the beginning, and at the end. Cute question... INkstAND......Time spent reading a question is usually more productive than time spent writing an answer! ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------------ From contact <@t> excaliburpathology.com Thu Jun 29 14:42:43 2006 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Thu Jun 29 14:42:51 2006 Subject: [Histonet] Feedback on Powerpath/Computer Documentation Message-ID: <20060629194243.27052.qmail@web50108.mail.yahoo.com> Hello, I wrote my own database with Microsoft Access. It comes bundled with Microsoft Office. I create reports, print labels, and bill with the original data input for the report. Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-759-3953 contact@excaliburpathology.com www.excaliburpathology.com From algranth <@t> u.arizona.edu Thu Jun 29 17:10:41 2006 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Thu Jun 29 17:10:48 2006 Subject: [Histonet] any Hungarian histotechs on the list? Message-ID: <4.3.2.7.2.20060629150733.021c5cb0@algranth.inbox.email.arizona.edu> Are there any histotechs on Histonet from Budapest? I have a friend who is a histotech and is interested in contacting them. Please respond to aselmeczi@hotmail.com Thanks!!! Andi ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From caron_fournier <@t> yahoo.ca Thu Jun 29 19:29:43 2006 From: caron_fournier <@t> yahoo.ca (caron fournier) Date: Thu Jun 29 19:29:47 2006 Subject: [Histonet] re: proteoglycans Message-ID: <20060630002943.61841.qmail@web35408.mail.mud.yahoo.com> I wanted to know if anyone has a method that can be used to demonstrate "quantitatively" how much proteoglycan there is in a vertebral disc? I know that there are some stains that can demonstrate Glucosaminglycans and proteoglycans but can they be quantitative? Or, is a spectrophotometer the only way to go? Thanks, Caron Caron Fournier, BSc, R.T. Department of Orthopaedics, Division of Orthopaedic Engineering Research, U.B.C. --------------------------------- Now you can have a huge leap forward in email: get the new Yahoo! Mail. From BMolinari <@t> heart.thi.tmc.edu Fri Jun 30 05:55:40 2006 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Jun 30 05:55:44 2006 Subject: [Histonet] Formula 83 Message-ID: Happy Friday. Has anyone tried this product? It is produced by CBG Biotech. Thanks and Happy 4th of July to those who ware taking a nice long weekend. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) From GoodwinD <@t> pahosp.com Fri Jun 30 08:36:04 2006 From: GoodwinD <@t> pahosp.com (Goodwin, Diana) Date: Fri Jun 30 08:36:12 2006 Subject: [Histonet] Tissue-Tek Express Message-ID: <80CDD9C3FEEAFD4982B114C4A6DFD00E02CB7F15@uphsmbx2.UPHS.PENNHEALTH.PRV> Greetings, Netters. Is anyone out there using this instrument, and if so, what are your thoughts on ROI? Thanks---and Happy Independence Day to all my fellow Americans! Diana Goodwin Supervisor, Anatomic Pathology Pennsylvania Hospital Preston 655-C ph. 215-829-6532 pager 215-422-5160 fax 215-829-7564 e-mail goodwind@[pahosp.com The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From kemlo.rogerson <@t> waht.swest.nhs.uk Fri Jun 30 08:52:29 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Jun 30 08:51:57 2006 Subject: [Histonet] Tissue-Tek Express Message-ID: Question? Can we say 'Happy Independence Day'? It was from us you were made independent from, so should we be sad? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 Distance is meant to relate, not separate. --Satish Kumar This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From ASelf <@t> gmhsc.com Fri Jun 30 09:47:27 2006 From: ASelf <@t> gmhsc.com (Amy Self) Date: Fri Jun 30 09:45:51 2006 Subject: [Histonet] Benchmark Procedures Message-ID: <39836CD6DB61654E8F95A35898C92186D71104@exchange.gmhpost.com> Hello Netters, Does anyone have policy/procedures to the Ventana benchmark that they would be willing to share with me. I have been out of work for the past 12 weeks and I am just returning to find out that all CAP procedures need to be done by july 25. Since we have just recently purchased the benchmark I have nothing but the operators manual. I hate to ask for this but right now I am feeling the heat from our CAP inspection and I need some help with writing procedures. Thanks in advance and I hope that everyone has a great weekend and a Happy 4th. Amy NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. From lblazek <@t> digestivespecialists.com Fri Jun 30 09:50:53 2006 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Jun 30 09:51:06 2006 Subject: [Histonet] Tissue-Tek Express Message-ID: <6CBA6DC98A079D408C87250591D9DFB802360DB4@bruexchange.digestivespecialists.com> Sadness in the way your child goes off into the world to start a life of their own. They still stay connected to the parent but are independent. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: Friday, June 30, 2006 9:52 AM To: 'Goodwin, Diana'; HistoNet@Pathology.swmed.edu Subject: RE: [Histonet] Tissue-Tek Express Question? Can we say 'Happy Independence Day'? It was from us you were made independent from, so should we be sad? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 Distance is meant to relate, not separate. --Satish Kumar This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Fri Jun 30 10:01:22 2006 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri Jun 30 10:03:13 2006 Subject: [Histonet] Tissue-Tek Express In-Reply-To: <6CBA6DC98A079D408C87250591D9DFB802360DB4@bruexchange.digestivespecialists.com> Message-ID: but happiness that you have helped to give them the tools to survive out there?? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Blazek, Linda Sent: 30 June 2006 15:51 To: Kemlo Rogerson; Goodwin, Diana; HistoNet@Pathology.swmed.edu Subject: RE: [Histonet] Tissue-Tek Express Sadness in the way your child goes off into the world to start a life of their own. They still stay connected to the parent but are independent. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: Friday, June 30, 2006 9:52 AM To: 'Goodwin, Diana'; HistoNet@Pathology.swmed.edu Subject: RE: [Histonet] Tissue-Tek Express Question? Can we say 'Happy Independence Day'? It was from us you were made independent from, so should we be sad? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 Distance is meant to relate, not separate. --Satish Kumar This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DennisH <@t> cookchildrens.org Fri Jun 30 10:56:28 2006 From: DennisH <@t> cookchildrens.org (Dennis Hahn) Date: Fri Jun 30 10:56:47 2006 Subject: [Histonet] Tissue-Tek Express Message-ID: Histo Net, Climbs upon his American, gun-toting soap-box.... Let the Friday flaming begin. This is not meant to offend any one nationality. I usually just pick up tid-bits of information from the histonet, and ignore all the usual comments or flamings. However, maybe the wording was not meant to be what I interpret it as, but... 1) We were not "made" independent from anyone. No other country, or king, gave us our independence. We fought for it. I served in our armed forces for 6 years active duty, including the "first" gulf "conflict" as it is referred to. I fly an American flag in my front yard and do my best to explain to my children each time we take it down and burn it (Yes, you SHOULD burn the American flag when it is worn out, and no longer usable...it represents our country, a living, breathing entity) that many men, and women, have died so that the people in our nation, and others, may vote for our representatives both locally and nationally, say what we want, when we want, come and go throughout our country and the world as we please, all without the fear of any retaliation when we lay our heads down at night to sleep because of it. 2) I'm not saying that anyone should be sad, but in less than 230 years, the US did GAIN it's independence, survive a brief civil war, establish a free, and hopefully, equal society, spearhead many of the industrial revolution ideas (That cotton gin thing really had the world stumped, didn't it?) and create the most sophisticated military the planet has ever known. Yes, we do have our problems...The elderly, poor, and many children do not have access to adequate health care. 3) I do believe that Diana Goodwin stated, and I quote, "Happy Independence Day to all my fellow Americans!" Why respond at all if it doesn't apply to you? 4) Let all of my fellow Americans remember ALL the soldiers this weekend that are currently scattered throughout the world. Many are in places and battles that we will never know about. Climbs down off his soapbox, gets some BBQ to eat, and is ready for fireworks. Dennis Hahn dennish@cookchildrens.org >>> Kemlo Rogerson 6/30/2006 8:52 AM >>> Question? Can we say 'Happy Independence Day'? It was from us you were made independent from, so should we be sad? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 Distance is meant to relate, not separate. --Satish Kumar This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------------------------------------ Cook Children's Health Care System This e-mail, facsimile, or letter and any files or attachments transmitted may contain information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Cook Children's Health Care System immediately at (682)885-4000 or via e-mail at compliance@cookchildrens.org. Cook Children's Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. ----------------------------------------------------------------------------------------------------------- From GoodwinD <@t> pahosp.com Fri Jun 30 11:07:51 2006 From: GoodwinD <@t> pahosp.com (Goodwin, Diana) Date: Fri Jun 30 11:08:02 2006 Subject: [Histonet] Tissue-Tek Express Message-ID: <80CDD9C3FEEAFD4982B114C4A6DFD00E02CB7F1C@uphsmbx2.UPHS.PENNHEALTH.PRV> Here, Here, and a resounding "Huzzah!" So nice that we bleeding-heart liberals can wholeheartedly agree and identify with our gun-toting GOP brethren now and again. Now I'm going home to hoist an ice-cold Coors and rent "The Patriot". Best Regards to ALL, Diana Goodwin ________________________________ From: Dennis Hahn [mailto:DennisH@cookchildrens.org] Sent: Friday, June 30, 2006 11:56 AM To: Goodwin, Diana; HistoNet@Pathology.swmed.edu; kemlo.rogerson@waht.swest.nhs.uk Subject: RE: [Histonet] Tissue-Tek Express Histo Net, Climbs upon his American, gun-toting soap-box.... Let the Friday flaming begin. This is not meant to offend any one nationality. I usually just pick up tid-bits of information from the histonet, and ignore all the usual comments or flamings. However, maybe the wording was not meant to be what I interpret it as, but... 1) We were not "made" independent from anyone. No other country, or king, gave us our independence. We fought for it. I served in our armed forces for 6 years active duty, including the "first" gulf "conflict" as it is referred to. I fly an American flag in my front yard and do my best to explain to my children each time we take it down and burn it (Yes, you SHOULD burn the American flag when it is worn out, and no longer usable...it represents our country, a living, breathing entity) that many men, and women, have died so that the people in our nation, and others, may vote for our representatives both locally and nationally, say what we want, when we want, come and go throughout our country and the world as we please, all without the fear of any retaliation when we lay our heads down at night to sleep because of it. 2) I'm not saying that anyone should be sad, but in less than 230 years, the US did GAIN it's independence, survive a brief civil war, establish a free, and hopefully, equal society, spearhead many of the industrial revolution ideas (That cotton gin thing really had the world stumped, didn't it?) and create the most sophisticated military the planet has ever known. Yes, we do have our problems...The elderly, poor, and many children do not have access to adequate health care. 3) I do believe that Diana Goodwin stated, and I quote, "Happy Independence Day to all my fellow Americans!" Why respond at all if it doesn't apply to you? 4) Let all of my fellow Americans remember ALL the soldiers this weekend that are currently scattered throughout the world. Many are in places and battles that we will never know about. Climbs down off his soapbox, gets some BBQ to eat, and is ready for fireworks. Dennis Hahn dennish@cookchildrens.org >>> Kemlo Rogerson 6/30/2006 8:52 AM >>> Question? Can we say 'Happy Independence Day'? It was from us you were made independent from, so should we be sad? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 Distance is meant to relate, not separate. --Satish Kumar This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ ------------------------------------ Cook Children's Health Care System This e-mail, facsimile, or letter and any files or attachments transmitted may contain information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Cook Children's Health Care System immediately at (682)885-4000 or via e-mail at compliance@cookchildrens.org. Cook Children's Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. ------------------------------------------------------------------------ ----------------------------------- The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From jqb7 <@t> cdc.gov Fri Jun 30 11:24:38 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/NCID/VR)) Date: Fri Jun 30 11:32:57 2006 Subject: [Histonet] Tissue-Tek Express Message-ID: Is it okay that I am GOP but don't have a gun? Jeanine Bartlett, BS, HT(ASCP) (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goodwin, Diana Sent: Friday, June 30, 2006 12:08 PM To: Dennis Hahn; HistoNet@Pathology.swmed.edu; kemlo.rogerson@waht.swest.nhs.uk Subject: RE: [Histonet] Tissue-Tek Express Here, Here, and a resounding "Huzzah!" So nice that we bleeding-heart liberals can wholeheartedly agree and identify with our gun-toting GOP brethren now and again. Now I'm going home to hoist an ice-cold Coors and rent "The Patriot". Best Regards to ALL, Diana Goodwin ________________________________ From: Dennis Hahn [mailto:DennisH@cookchildrens.org] Sent: Friday, June 30, 2006 11:56 AM To: Goodwin, Diana; HistoNet@Pathology.swmed.edu; kemlo.rogerson@waht.swest.nhs.uk Subject: RE: [Histonet] Tissue-Tek Express Histo Net, Climbs upon his American, gun-toting soap-box.... Let the Friday flaming begin. This is not meant to offend any one nationality. I usually just pick up tid-bits of information from the histonet, and ignore all the usual comments or flamings. However, maybe the wording was not meant to be what I interpret it as, but... 1) We were not "made" independent from anyone. No other country, or king, gave us our independence. We fought for it. I served in our armed forces for 6 years active duty, including the "first" gulf "conflict" as it is referred to. I fly an American flag in my front yard and do my best to explain to my children each time we take it down and burn it (Yes, you SHOULD burn the American flag when it is worn out, and no longer usable...it represents our country, a living, breathing entity) that many men, and women, have died so that the people in our nation, and others, may vote for our representatives both locally and nationally, say what we want, when we want, come and go throughout our country and the world as we please, all without the fear of any retaliation when we lay our heads down at night to sleep because of it. 2) I'm not saying that anyone should be sad, but in less than 230 years, the US did GAIN it's independence, survive a brief civil war, establish a free, and hopefully, equal society, spearhead many of the industrial revolution ideas (That cotton gin thing really had the world stumped, didn't it?) and create the most sophisticated military the planet has ever known. Yes, we do have our problems...The elderly, poor, and many children do not have access to adequate health care. 3) I do believe that Diana Goodwin stated, and I quote, "Happy Independence Day to all my fellow Americans!" Why respond at all if it doesn't apply to you? 4) Let all of my fellow Americans remember ALL the soldiers this weekend that are currently scattered throughout the world. Many are in places and battles that we will never know about. Climbs down off his soapbox, gets some BBQ to eat, and is ready for fireworks. Dennis Hahn dennish@cookchildrens.org >>> Kemlo Rogerson 6/30/2006 8:52 AM >>> Question? Can we say 'Happy Independence Day'? It was from us you were made independent from, so should we be sad? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 Distance is meant to relate, not separate. --Satish Kumar This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ ------------------------------------ Cook Children's Health Care System This e-mail, facsimile, or letter and any files or attachments transmitted may contain information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Cook Children's Health Care System immediately at (682)885-4000 or via e-mail at compliance@cookchildrens.org. Cook Children's Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. ------------------------------------------------------------------------ ----------------------------------- The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mfredrickson <@t> cohenderm.com Fri Jun 30 11:47:47 2006 From: mfredrickson <@t> cohenderm.com (Michael Fredrickson) Date: Fri Jun 30 11:47:44 2006 Subject: [Histonet] PCR for Mycobacteria on FFPE Message-ID: We are trying to locate a commercial / clinical laboratory that performs PCR on formalin-fixed paraffin embedding tissue for Mycobacteria. Any help would be appreciated. Thanks. Michael M. Fredrickson mfredrickson@cohenderm.com From timothy.macatee <@t> med.nyu.edu Fri Jun 30 11:49:23 2006 From: timothy.macatee <@t> med.nyu.edu (Timothy Macatee) Date: Fri Jun 30 11:51:41 2006 Subject: [Histonet] Tissue-Tek Express In-Reply-To: Message-ID: Just for anyone who is interested, I found this web site on the American Flag a nice overview. Happy Independent 4th. http://www.ushistory.org/betsy/flagetiq.html#1 Tim On 6/30/06 12:24 PM, "Bartlett, Jeanine (CDC/NCID/VR)" wrote: > Is it okay that I am GOP but don't have a gun? > > > Jeanine Bartlett, BS, HT(ASCP) > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goodwin, > Diana > Sent: Friday, June 30, 2006 12:08 PM > To: Dennis Hahn; HistoNet@Pathology.swmed.edu; > kemlo.rogerson@waht.swest.nhs.uk > Subject: RE: [Histonet] Tissue-Tek Express > > Here, Here, and a resounding "Huzzah!" So nice that we bleeding-heart > liberals can wholeheartedly agree and identify with our gun-toting GOP > brethren now and again. > > Now I'm going home to hoist an ice-cold Coors and rent "The Patriot". > > Best Regards to ALL, > Diana Goodwin > > ________________________________ > > From: Dennis Hahn [mailto:DennisH@cookchildrens.org] > Sent: Friday, June 30, 2006 11:56 AM > To: Goodwin, Diana; HistoNet@Pathology.swmed.edu; > kemlo.rogerson@waht.swest.nhs.uk > Subject: RE: [Histonet] Tissue-Tek Express > > > Histo Net, > > Climbs upon his American, gun-toting soap-box.... > > Let the Friday flaming begin. This is not meant to offend any one > nationality. I usually just pick up tid-bits of information from the > histonet, and ignore all the usual comments or flamings. > > However, maybe the wording was not meant to be what I interpret it as, > but... > > 1) We were not "made" independent from anyone. No other country, or > king, gave us our independence. We fought for it. I served in our armed > forces for 6 years active duty, including the "first" gulf "conflict" as > it is referred to. I fly an American flag in my front yard and do my > best to explain to my children each time we take it down and burn it > (Yes, you SHOULD burn the American flag when it is worn out, and no > longer usable...it represents our country, a living, breathing entity) > that many men, and women, have died so that the people in our nation, > and others, may vote for our representatives both locally and > nationally, say what we want, when we want, come and go throughout our > country and the world as we please, all without the fear of any > retaliation when we lay our heads down at night to sleep because of it. > > 2) I'm not saying that anyone should be sad, but in less than 230 years, > the US did GAIN it's independence, survive a brief civil war, establish > a free, and hopefully, equal society, spearhead many of the industrial > revolution ideas (That cotton gin thing really had the world stumped, > didn't it?) and create the most sophisticated military the planet has > ever known. Yes, we do have our problems...The elderly, poor, and many > children do not have access to adequate health care. > > 3) I do believe that Diana Goodwin stated, and I quote, "Happy > Independence Day to all my fellow Americans!" Why respond at all if it > doesn't apply to you? > > 4) Let all of my fellow Americans remember ALL the soldiers this weekend > that are currently scattered throughout the world. Many are in places > and battles that we will never know about. > > Climbs down off his soapbox, gets some BBQ to eat, and is ready for > fireworks. > > Dennis Hahn > dennish@cookchildrens.org > > >>>> Kemlo Rogerson 6/30/2006 8:52 AM >>>> > > Question? Can we say 'Happy Independence Day'? It was from us you were > made > independent from, so should we be sad? > > Kemlo Rogerson > Pathology Manager > Ext 3311 > DD 01934 647057 > Mob 07749 754194 > Pager 07659 597107 > > Distance is meant to relate, not separate. --Satish Kumar > > > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on > its > contents: to do so is strictly prohibited and may be unlawful. Please > inform > me that this message has gone astray before deleting it. Thank you for > your > co-operation > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------------------------------------------------ > ------------------------------------ > Cook Children's Health Care System > > This e-mail, facsimile, or letter and any files or attachments > transmitted may contain information that is confidential and privileged. > This information is intended only for the use of the individual(s) and > entity(ies) to whom it is addressed. If you are the intended recipient, > further disclosures are prohibited without proper authorization. If you > are not the intended recipient, any disclosure, copying, printing, or > use of this information is strictly prohibited and possibly a violation > of federal or state law and regulations. > > If you have received this information in error, please notify Cook > Children's Health Care System immediately at (682)885-4000 or via e-mail > at compliance@cookchildrens.org. Cook Children's Health Care System, its > subsidiaries, and affiliates hereby claim all applicable privileges > related to this information. > ------------------------------------------------------------------------ > ----------------------------------- > > > The information contained in this e-mail message is intended only for > the personal and confidential use of the recipient(s) named above. If > the reader of this message is not the intended recipient or an agent > responsible for delivering it to the intended recipient, you are hereby > notified that you have received this document in error and that any > review, dissemination, distribution, or copying of this message is > strictly prohibited. If you have received this communication in error, > please notify us immediately by e-mail, and delete the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Tim Macatee Research Histology Core New York University School of Medicine 550 First Ave. Department of Pathology. Medical Science Building - Room 521 New York, N.Y. 10016 (212) 263-3888 From jcline <@t> wchsys.org Fri Jun 30 11:57:04 2006 From: jcline <@t> wchsys.org (Joyce Cline) Date: Fri Jun 30 11:57:17 2006 Subject: [Histonet] RE: Formula 83 In-Reply-To: Message-ID: <000601c69c66$371ccf30$1d2a14ac@wchsys.org> I use Formula 83 in my processor, staining and coverslipping of H&E and IHC. I find it works well, better than the former substitute I was using. Also, dries real quickly and recycles well. ------------------------------------------------------------------------ ---- Has anyone tried this product? It is produced by CBG Biotech. Thanks and Happy 4th of July to those who ware taking a nice long weekend. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From limla <@t> mail.nih.gov Fri Jun 30 12:03:16 2006 From: limla <@t> mail.nih.gov (Lim, Langston (NIH/NCI) [E]) Date: Fri Jun 30 12:03:24 2006 Subject: [Histonet] Tissue-Tek Express In-Reply-To: Message-ID: To my fellow Americans and veterans: If tomorrow all the things were gone I'd worked for all my life, And I had to start again with just my children and my wife. I'd thank my lucky stars to be living here today, 'Cause the flag still stands for freedom and they can't take that away. And I'm proud to be an American where at least I know I'm free. And I won't forget the men who died, who gave that right to me. And I'd gladly stand up next to you and defend her still today. 'Cause there ain't no doubt I love this land God bless the U.S.A. >From the lakes of Minnesota, to the hills of Tennessee, across the plains of Texas, from sea to shining sea, >From Detroit down to Houston and New York to LA, Well, there's pride in every American heart, and it's time to stand and say: I'm proud to be an American where at least I know I'm free. And I won't forget the men who died, who gave that right to me. And I'd gladly stand up next to you and defend her still today. 'Cause there ain't no doubt I love this land God bless the U.S.A. Langston Lim, HT (ASCP) Tissue Array Research Program DHHS/NIH/NCI/CCR/LP -----Original Message----- From: Timothy Macatee [mailto:timothy.macatee@med.nyu.edu] Sent: Friday, June 30, 2006 12:49 PM To: Bartlett, Jeanine H. (CDC); Goodwin, Diana; Dennis Hahn; HistoNet@Pathology.swmed.edu; kemlo.rogerson@waht.swest.nhs.uk Subject: Re: [Histonet] Tissue-Tek Express Just for anyone who is interested, I found this web site on the American Flag a nice overview. Happy Independent 4th. http://www.ushistory.org/betsy/flagetiq.html#1 Tim On 6/30/06 12:24 PM, "Bartlett, Jeanine (CDC/NCID/VR)" wrote: > Is it okay that I am GOP but don't have a gun? > > > Jeanine Bartlett, BS, HT(ASCP) > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goodwin, > Diana > Sent: Friday, June 30, 2006 12:08 PM > To: Dennis Hahn; HistoNet@Pathology.swmed.edu; > kemlo.rogerson@waht.swest.nhs.uk > Subject: RE: [Histonet] Tissue-Tek Express > > Here, Here, and a resounding "Huzzah!" So nice that we bleeding-heart > liberals can wholeheartedly agree and identify with our gun-toting GOP > brethren now and again. > > Now I'm going home to hoist an ice-cold Coors and rent "The Patriot". > > Best Regards to ALL, > Diana Goodwin > > ________________________________ > > From: Dennis Hahn [mailto:DennisH@cookchildrens.org] > Sent: Friday, June 30, 2006 11:56 AM > To: Goodwin, Diana; HistoNet@Pathology.swmed.edu; > kemlo.rogerson@waht.swest.nhs.uk > Subject: RE: [Histonet] Tissue-Tek Express > > > Histo Net, > > Climbs upon his American, gun-toting soap-box.... > > Let the Friday flaming begin. This is not meant to offend any one > nationality. I usually just pick up tid-bits of information from the > histonet, and ignore all the usual comments or flamings. > > However, maybe the wording was not meant to be what I interpret it as, > but... > > 1) We were not "made" independent from anyone. No other country, or > king, gave us our independence. We fought for it. I served in our armed > forces for 6 years active duty, including the "first" gulf "conflict" as > it is referred to. I fly an American flag in my front yard and do my > best to explain to my children each time we take it down and burn it > (Yes, you SHOULD burn the American flag when it is worn out, and no > longer usable...it represents our country, a living, breathing entity) > that many men, and women, have died so that the people in our nation, > and others, may vote for our representatives both locally and > nationally, say what we want, when we want, come and go throughout our > country and the world as we please, all without the fear of any > retaliation when we lay our heads down at night to sleep because of it. > > 2) I'm not saying that anyone should be sad, but in less than 230 years, > the US did GAIN it's independence, survive a brief civil war, establish > a free, and hopefully, equal society, spearhead many of the industrial > revolution ideas (That cotton gin thing really had the world stumped, > didn't it?) and create the most sophisticated military the planet has > ever known. Yes, we do have our problems...The elderly, poor, and many > children do not have access to adequate health care. > > 3) I do believe that Diana Goodwin stated, and I quote, "Happy > Independence Day to all my fellow Americans!" Why respond at all if it > doesn't apply to you? > > 4) Let all of my fellow Americans remember ALL the soldiers this weekend > that are currently scattered throughout the world. Many are in places > and battles that we will never know about. > > Climbs down off his soapbox, gets some BBQ to eat, and is ready for > fireworks. > > Dennis Hahn > dennish@cookchildrens.org > > >>>> Kemlo Rogerson 6/30/2006 8:52 AM >>>> > > Question? Can we say 'Happy Independence Day'? It was from us you were > made > independent from, so should we be sad? > > Kemlo Rogerson > Pathology Manager > Ext 3311 > DD 01934 647057 > Mob 07749 754194 > Pager 07659 597107 > > Distance is meant to relate, not separate. --Satish Kumar > > > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on > its > contents: to do so is strictly prohibited and may be unlawful. Please > inform > me that this message has gone astray before deleting it. Thank you for > your > co-operation > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------------------------------------------------ > ------------------------------------ > Cook Children's Health Care System > > This e-mail, facsimile, or letter and any files or attachments > transmitted may contain information that is confidential and privileged. > This information is intended only for the use of the individual(s) and > entity(ies) to whom it is addressed. If you are the intended recipient, > further disclosures are prohibited without proper authorization. If you > are not the intended recipient, any disclosure, copying, printing, or > use of this information is strictly prohibited and possibly a violation > of federal or state law and regulations. > > If you have received this information in error, please notify Cook > Children's Health Care System immediately at (682)885-4000 or via e-mail > at compliance@cookchildrens.org. Cook Children's Health Care System, its > subsidiaries, and affiliates hereby claim all applicable privileges > related to this information. > ------------------------------------------------------------------------ > ----------------------------------- > > > The information contained in this e-mail message is intended only for > the personal and confidential use of the recipient(s) named above. If > the reader of this message is not the intended recipient or an agent > responsible for delivering it to the intended recipient, you are hereby > notified that you have received this document in error and that any > review, dissemination, distribution, or copying of this message is > strictly prohibited. If you have received this communication in error, > please notify us immediately by e-mail, and delete the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Tim Macatee Research Histology Core New York University School of Medicine 550 First Ave. Department of Pathology. Medical Science Building - Room 521 New York, N.Y. 10016 (212) 263-3888 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GoodwinD <@t> pahosp.com Fri Jun 30 12:13:22 2006 From: GoodwinD <@t> pahosp.com (Goodwin, Diana) Date: Fri Jun 30 12:13:31 2006 Subject: [Histonet] Feedback Re: Tissue-Tek Express Instrument Message-ID: <80CDD9C3FEEAFD4982B114C4A6DFD00E02CB7F21@uphsmbx2.UPHS.PENNHEALTH.PRV> Since my original post was turned into a political debate, I will try again to solicit the information that I am interested in obtaining: Is anyone out there using this instrument, and if so, what are your thoughts on the return on investment: functionality, cost savings, quality compared to overnight, any savings in tech-time, etc. Thanks again, Diana Goodwin Supervisor, Anatomic Pathology Pennsylvania Hospital Preston 655-C ph. 215-829-6532 pager 215-422-5160 fax 215-829-7564 e-mail goodwind@[pahosp.com The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From kerry.l.crabb <@t> gsk.com Fri Jun 30 12:42:08 2006 From: kerry.l.crabb <@t> gsk.com (kerry.l.crabb@gsk.com) Date: Fri Jun 30 12:42:23 2006 Subject: [Histonet] Kerry L Crabb/PharmRD/GSK is out of the office. Message-ID: I will be out of the office starting 30-Jun-2006 and will not return until 10-Jul-2006. During my absence contact Eve about necropsy issues and contact Teresa about histology issues. I will respond to messages when I return. From mhorne <@t> upei.ca Fri Jun 30 13:58:47 2006 From: mhorne <@t> upei.ca (Margaret Horne) Date: Fri Jun 30 13:01:44 2006 Subject: [Histonet] To Any Canadians out there. Message-ID: <44A53C26.12146.12ACD68@localhost> Happy Canada Day!! Hope the weather is good and your day as sweet as maple syrup. :-) Margaret Margaret Horne , Histology Teaching Assistant, Dept. of B.SC., Atlantic Veterinary College, U.P.E.I., 550 University Ave., Charlottetown, P.E.I., C1A 4P3 Canada From john_ronan <@t> merck.com Fri Jun 30 13:02:45 2006 From: john_ronan <@t> merck.com (Ronan, John) Date: Fri Jun 30 13:03:08 2006 Subject: [Histonet] More feedback on Powerpath - Database Programs Message-ID: I work in a research setting and am not familiar with Powerpath. I have used filemaker Pro to create my own database program to accession and track tissue. My program allows me to associate pictures and histopath reports with accession numbers. It is a very user friendly and flexible program that can probably fit your needs. It is also very easy to modify so you could start very simple and become more sophisticated as you become more comfortable with the software. It is a few hundred dollars to purchase. ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------------ From HDOWNS <@t> PARTNERS.ORG Fri Jun 30 13:14:04 2006 From: HDOWNS <@t> PARTNERS.ORG (Downs, Heather M.) Date: Fri Jun 30 13:14:13 2006 Subject: [Histonet] PowerPath/Computer? Message-ID: <54DC0778AC30D4418FFF52A7FD2008750220B286@PHSXMB19.partners.org> We use both powerpath for a small amount of clinical specimens, and the bulk of our research tissue goes into to an Access program. It is very simple to use, and can genearte all types of reports, custom designed for you needs. Heather MGH Nerve Injury Unit -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, June 30, 2006 1:01 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 31, Issue 43 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re:Gr-1 staining in mouse spleen (Herber, Donna L.) (Jamie E Erickson) 2. Feedback on Powerpath/Computer Documentation (Reuel Cornelia) 3. TRIVIA CHALLENGE OF THE DAY: (Ronan, John) 4. Feedback on Powerpath/Computer Documentation (Paula Pierce) 5. any Hungarian histotechs on the list? (Andrea Grantham) 6. re: proteoglycans (caron fournier) 7. Formula 83 (Molinari, Betsy) 8. Tissue-Tek Express (Goodwin, Diana) 9. RE: Tissue-Tek Express (Kemlo Rogerson) 10. Benchmark Procedures (Amy Self) 11. RE: Tissue-Tek Express (Blazek, Linda) 12. RE: Tissue-Tek Express (Edwards, R.E.) 13. RE: Tissue-Tek Express (Dennis Hahn) 14. RE: Tissue-Tek Express (Goodwin, Diana) 15. RE: Tissue-Tek Express (Bartlett, Jeanine (CDC/NCID/VR)) 16. PCR for Mycobacteria on FFPE (Michael Fredrickson) 17. Re: Tissue-Tek Express (Timothy Macatee) 18. RE: Formula 83 (Joyce Cline) ---------------------------------------------------------------------- Message: 1 Date: Thu, 29 Jun 2006 13:06:37 -0400 From: Jamie E Erickson Subject: [Histonet] Re:Gr-1 staining in mouse spleen (Herber, Donna L.) To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" HI Donna, I've had great results in mouse granulocytic tumors with Gr-1 but my protocol was very standard you can check out the protocol in this journal article. Kyriaki Dunussi-Joannopoulos, Jamie Erickson et al... Efficacious immunomodulatory activity of the chemokine stromal cell-derived factor 1 (SDF-1): local secretion of SDF-1 at the tumor site serves as T-cell chemoattractant and mediates T-cell-dependent antitumor responses. Blood 100(5): 1551-1558, 2002 Sept. I used frozen sections and the Gr-1 antibody from BD @ 2ug/ml (LY-6G), RB6-8C5 Catalog #: 550291 (FOR IHC) we used this to check that granulocytes in and around that tumor. I think you may be using the wrong antibody this one is stated to work in IHC by BD( CAT # 550291,RB68C5) and is the same clone I use. You might want to look into that. Jamie _______________________________ Jamie Erickson Sr. Research Associate Department: DSMP Abbott Bioresearch Center 100 Research Drive Worcester, MA 01605-4341 508-688-3134 FAX: 508-793-4895 e-mail: jamie.erickson@abbott.com ------------------------------ Message: 2 Date: Thu, 29 Jun 2006 13:11:09 -0500 From: "Reuel Cornelia" Subject: [Histonet] Feedback on Powerpath/Computer Documentation To: Message-ID: Content-Type: text/plain; charset=US-ASCII Is it worth using Powerpath(Tamtron) if you do not have enough clinical cases but rather used it more for research accession. Is it worth the service maintenance fee($17,000) I paid per year for research accession and not for clinical cases which the software was built for. Do you have any suggestion of which other software that will meet our needs without paying too much with service maintenance lesser than we have or you only pay for the installation without maintenance fee(Just wonderin if there is anything like this). Your opinion is highly appreciated.Thank you. reuel TSRH dallas,TX ******************************************************************************** *********************************** Texas Scottish Rite Hospital for Children is one of the nation's leading pediatric centers for the treatment of orthopedic conditions and learning disorders, like dyslexia. This email transmission and/or its attachments may contain confidential health information, intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing it to any other party unless required to do so by law and is required to delete/destroy the information after its stated need has been fulfilled. If you are not the intended recipient, any disclosure, copying, distribution or action taken in reliance on the contents of this email transmission is prohibited. If you have received this information in error, please notify the sender immediately and delete this information. We appreciate your efforts to protect the children's confidential information. ******************************************************************************** *********************************** ------------------------------ Message: 3 Date: Thu, 29 Jun 2006 14:46:50 -0400 From: "Ronan, John" Subject: [Histonet] TRIVIA CHALLENGE OF THE DAY: To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain TRIVIA CHALLENGE OF THE DAY: Name one eight letter word that has kst in the middle, in the beginning, and at the end. Cute question... INkstAND......Time spent reading a question is usually more productive than time spent writing an answer! ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------------ ------------------------------ Message: 4 Date: Thu, 29 Jun 2006 12:42:43 -0700 (PDT) From: Paula Pierce Subject: [Histonet] Feedback on Powerpath/Computer Documentation To: histonet@lists.utsouthwestern.edu Message-ID: <20060629194243.27052.qmail@web50108.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello, I wrote my own database with Microsoft Access. It comes bundled with Microsoft Office. I create reports, print labels, and bill with the original data input for the report. Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-759-3953 contact@excaliburpathology.com www.excaliburpathology.com ------------------------------ Message: 5 Date: Thu, 29 Jun 2006 15:10:41 -0700 From: Andrea Grantham Subject: [Histonet] any Hungarian histotechs on the list? To: histonet@lists.utsouthwestern.edu Message-ID: <4.3.2.7.2.20060629150733.021c5cb0@algranth.inbox.email.arizona.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Are there any histotechs on Histonet from Budapest? I have a friend who is a histotech and is interested in contacting them. Please respond to aselmeczi@hotmail.com Thanks!!! Andi ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html ------------------------------ Message: 6 Date: Thu, 29 Jun 2006 20:29:43 -0400 (EDT) From: caron fournier Subject: [Histonet] re: proteoglycans To: histonet@lists.utsouthwestern.edu Message-ID: <20060630002943.61841.qmail@web35408.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I wanted to know if anyone has a method that can be used to demonstrate "quantitatively" how much proteoglycan there is in a vertebral disc? I know that there are some stains that can demonstrate Glucosaminglycans and proteoglycans but can they be quantitative? Or, is a spectrophotometer the only way to go? Thanks, Caron Caron Fournier, BSc, R.T. Department of Orthopaedics, Division of Orthopaedic Engineering Research, U.B.C. --------------------------------- Now you can have a huge leap forward in email: get the new Yahoo! Mail. ------------------------------ Message: 7 Date: Fri, 30 Jun 2006 05:55:40 -0500 From: "Molinari, Betsy" Subject: [Histonet] Formula 83 To: Message-ID: Content-Type: text/plain; charset="us-ascii" Happy Friday. Has anyone tried this product? It is produced by CBG Biotech. Thanks and Happy 4th of July to those who ware taking a nice long weekend. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) ------------------------------ Message: 8 Date: Fri, 30 Jun 2006 09:36:04 -0400 From: "Goodwin, Diana" Subject: [Histonet] Tissue-Tek Express To: Message-ID: <80CDD9C3FEEAFD4982B114C4A6DFD00E02CB7F15@uphsmbx2.UPHS.PENNHEALTH.PRV> Content-Type: text/plain; charset="us-ascii" Greetings, Netters. Is anyone out there using this instrument, and if so, what are your thoughts on ROI? Thanks---and Happy Independence Day to all my fellow Americans! Diana Goodwin Supervisor, Anatomic Pathology Pennsylvania Hospital Preston 655-C ph. 215-829-6532 pager 215-422-5160 fax 215-829-7564 e-mail goodwind@[pahosp.com The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ------------------------------ Message: 9 Date: Fri, 30 Jun 2006 14:52:29 +0100 From: Kemlo Rogerson Subject: RE: [Histonet] Tissue-Tek Express To: "'Goodwin, Diana'" , HistoNet@Pathology.swmed.edu Message-ID: Content-Type: text/plain Question? Can we say 'Happy Independence Day'? It was from us you were made independent from, so should we be sad? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 Distance is meant to relate, not separate. --Satish Kumar This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation ------------------------------ Message: 10 Date: Fri, 30 Jun 2006 10:47:27 -0400 From: "Amy Self" Subject: [Histonet] Benchmark Procedures To: Message-ID: <39836CD6DB61654E8F95A35898C92186D71104@exchange.gmhpost.com> Content-Type: text/plain; charset="iso-8859-1" Hello Netters, Does anyone have policy/procedures to the Ventana benchmark that they would be willing to share with me. I have been out of work for the past 12 weeks and I am just returning to find out that all CAP procedures need to be done by july 25. Since we have just recently purchased the benchmark I have nothing but the operators manual. I hate to ask for this but right now I am feeling the heat from our CAP inspection and I need some help with writing procedures. Thanks in advance and I hope that everyone has a great weekend and a Happy 4th. Amy NOTE: The information contained in this message may be privileged, confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to this message and deleting it from your computer. Thank you. ------------------------------ Message: 11 Date: Fri, 30 Jun 2006 10:50:53 -0400 From: "Blazek, Linda" Subject: RE: [Histonet] Tissue-Tek Express To: "Kemlo Rogerson" , "Goodwin, Diana" , Message-ID: <6CBA6DC98A079D408C87250591D9DFB802360DB4@bruexchange.digestivespecialists.com> Content-Type: text/plain; charset="us-ascii" Sadness in the way your child goes off into the world to start a life of their own. They still stay connected to the parent but are independent. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: Friday, June 30, 2006 9:52 AM To: 'Goodwin, Diana'; HistoNet@Pathology.swmed.edu Subject: RE: [Histonet] Tissue-Tek Express Question? Can we say 'Happy Independence Day'? It was from us you were made independent from, so should we be sad? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 Distance is meant to relate, not separate. --Satish Kumar This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Fri, 30 Jun 2006 16:01:22 +0100 From: "Edwards, R.E." Subject: RE: [Histonet] Tissue-Tek Express To: "Blazek, Linda" , "Kemlo Rogerson" , "Goodwin, Diana" , Message-ID: Content-Type: text/plain; charset="iso-8859-1" but happiness that you have helped to give them the tools to survive out there?? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Blazek, Linda Sent: 30 June 2006 15:51 To: Kemlo Rogerson; Goodwin, Diana; HistoNet@Pathology.swmed.edu Subject: RE: [Histonet] Tissue-Tek Express Sadness in the way your child goes off into the world to start a life of their own. They still stay connected to the parent but are independent. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: Friday, June 30, 2006 9:52 AM To: 'Goodwin, Diana'; HistoNet@Pathology.swmed.edu Subject: RE: [Histonet] Tissue-Tek Express Question? Can we say 'Happy Independence Day'? It was from us you were made independent from, so should we be sad? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 Distance is meant to relate, not separate. --Satish Kumar This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Fri, 30 Jun 2006 10:56:28 -0500 From: "Dennis Hahn" Subject: RE: [Histonet] Tissue-Tek Express To: ,, Message-ID: Content-Type: text/plain; charset=US-ASCII Histo Net, Climbs upon his American, gun-toting soap-box.... Let the Friday flaming begin. This is not meant to offend any one nationality. I usually just pick up tid-bits of information from the histonet, and ignore all the usual comments or flamings. However, maybe the wording was not meant to be what I interpret it as, but... 1) We were not "made" independent from anyone. No other country, or king, gave us our independence. We fought for it. I served in our armed forces for 6 years active duty, including the "first" gulf "conflict" as it is referred to. I fly an American flag in my front yard and do my best to explain to my children each time we take it down and burn it (Yes, you SHOULD burn the American flag when it is worn out, and no longer usable...it represents our country, a living, breathing entity) that many men, and women, have died so that the people in our nation, and others, may vote for our representatives both locally and nationally, say what we want, when we want, come and go throughout our country and the world as we please, all without the fear of any retaliation when we lay our heads down at night to sleep because of it. 2) I'm not saying that anyone should be sad, but in less than 230 years, the US did GAIN it's independence, survive a brief civil war, establish a free, and hopefully, equal society, spearhead many of the industrial revolution ideas (That cotton gin thing really had the world stumped, didn't it?) and create the most sophisticated military the planet has ever known. Yes, we do have our problems...The elderly, poor, and many children do not have access to adequate health care. 3) I do believe that Diana Goodwin stated, and I quote, "Happy Independence Day to all my fellow Americans!" Why respond at all if it doesn't apply to you? 4) Let all of my fellow Americans remember ALL the soldiers this weekend that are currently scattered throughout the world. Many are in places and battles that we will never know about. Climbs down off his soapbox, gets some BBQ to eat, and is ready for fireworks. Dennis Hahn dennish@cookchildrens.org >>> Kemlo Rogerson 6/30/2006 8:52 AM >>> Question? Can we say 'Happy Independence Day'? It was from us you were made independent from, so should we be sad? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 Distance is meant to relate, not separate. --Satish Kumar This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------------- ---------------------------- Cook Children's Health Care System This e-mail, facsimile, or letter and any files or attachments transmitted may contain information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Cook Children's Health Care System immediately at (682)885-4000 or via e-mail at compliance@cookchildrens.org. Cook Children's Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. -------------------------------------------------------------------------------- --------------------------- ------------------------------ Message: 14 Date: Fri, 30 Jun 2006 12:07:51 -0400 From: "Goodwin, Diana" Subject: RE: [Histonet] Tissue-Tek Express To: "Dennis Hahn" , , Message-ID: <80CDD9C3FEEAFD4982B114C4A6DFD00E02CB7F1C@uphsmbx2.UPHS.PENNHEALTH.PRV> Content-Type: text/plain; charset="us-ascii" Here, Here, and a resounding "Huzzah!" So nice that we bleeding-heart liberals can wholeheartedly agree and identify with our gun-toting GOP brethren now and again. Now I'm going home to hoist an ice-cold Coors and rent "The Patriot". Best Regards to ALL, Diana Goodwin ________________________________ From: Dennis Hahn [mailto:DennisH@cookchildrens.org] Sent: Friday, June 30, 2006 11:56 AM To: Goodwin, Diana; HistoNet@Pathology.swmed.edu; kemlo.rogerson@waht.swest.nhs.uk Subject: RE: [Histonet] Tissue-Tek Express Histo Net, Climbs upon his American, gun-toting soap-box.... Let the Friday flaming begin. This is not meant to offend any one nationality. I usually just pick up tid-bits of information from the histonet, and ignore all the usual comments or flamings. However, maybe the wording was not meant to be what I interpret it as, but... 1) We were not "made" independent from anyone. No other country, or king, gave us our independence. We fought for it. I served in our armed forces for 6 years active duty, including the "first" gulf "conflict" as it is referred to. I fly an American flag in my front yard and do my best to explain to my children each time we take it down and burn it (Yes, you SHOULD burn the American flag when it is worn out, and no longer usable...it represents our country, a living, breathing entity) that many men, and women, have died so that the people in our nation, and others, may vote for our representatives both locally and nationally, say what we want, when we want, come and go throughout our country and the world as we please, all without the fear of any retaliation when we lay our heads down at night to sleep because of it. 2) I'm not saying that anyone should be sad, but in less than 230 years, the US did GAIN it's independence, survive a brief civil war, establish a free, and hopefully, equal society, spearhead many of the industrial revolution ideas (That cotton gin thing really had the world stumped, didn't it?) and create the most sophisticated military the planet has ever known. Yes, we do have our problems...The elderly, poor, and many children do not have access to adequate health care. 3) I do believe that Diana Goodwin stated, and I quote, "Happy Independence Day to all my fellow Americans!" Why respond at all if it doesn't apply to you? 4) Let all of my fellow Americans remember ALL the soldiers this weekend that are currently scattered throughout the world. Many are in places and battles that we will never know about. Climbs down off his soapbox, gets some BBQ to eat, and is ready for fireworks. Dennis Hahn dennish@cookchildrens.org >>> Kemlo Rogerson 6/30/2006 8:52 AM >>> Question? Can we say 'Happy Independence Day'? It was from us you were made independent from, so should we be sad? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 Distance is meant to relate, not separate. --Satish Kumar This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ ------------------------------------ Cook Children's Health Care System This e-mail, facsimile, or letter and any files or attachments transmitted may contain information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Cook Children's Health Care System immediately at (682)885-4000 or via e-mail at compliance@cookchildrens.org. Cook Children's Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. ------------------------------------------------------------------------ ----------------------------------- The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. ------------------------------ Message: 15 Date: Fri, 30 Jun 2006 12:24:38 -0400 From: "Bartlett, Jeanine \(CDC/NCID/VR\)" Subject: RE: [Histonet] Tissue-Tek Express To: "Goodwin, Diana" , "Dennis Hahn" , , Message-ID: Content-Type: text/plain; charset="us-ascii" Is it okay that I am GOP but don't have a gun? Jeanine Bartlett, BS, HT(ASCP) (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goodwin, Diana Sent: Friday, June 30, 2006 12:08 PM To: Dennis Hahn; HistoNet@Pathology.swmed.edu; kemlo.rogerson@waht.swest.nhs.uk Subject: RE: [Histonet] Tissue-Tek Express Here, Here, and a resounding "Huzzah!" So nice that we bleeding-heart liberals can wholeheartedly agree and identify with our gun-toting GOP brethren now and again. Now I'm going home to hoist an ice-cold Coors and rent "The Patriot". Best Regards to ALL, Diana Goodwin ________________________________ From: Dennis Hahn [mailto:DennisH@cookchildrens.org] Sent: Friday, June 30, 2006 11:56 AM To: Goodwin, Diana; HistoNet@Pathology.swmed.edu; kemlo.rogerson@waht.swest.nhs.uk Subject: RE: [Histonet] Tissue-Tek Express Histo Net, Climbs upon his American, gun-toting soap-box.... Let the Friday flaming begin. This is not meant to offend any one nationality. I usually just pick up tid-bits of information from the histonet, and ignore all the usual comments or flamings. However, maybe the wording was not meant to be what I interpret it as, but... 1) We were not "made" independent from anyone. No other country, or king, gave us our independence. We fought for it. I served in our armed forces for 6 years active duty, including the "first" gulf "conflict" as it is referred to. I fly an American flag in my front yard and do my best to explain to my children each time we take it down and burn it (Yes, you SHOULD burn the American flag when it is worn out, and no longer usable...it represents our country, a living, breathing entity) that many men, and women, have died so that the people in our nation, and others, may vote for our representatives both locally and nationally, say what we want, when we want, come and go throughout our country and the world as we please, all without the fear of any retaliation when we lay our heads down at night to sleep because of it. 2) I'm not saying that anyone should be sad, but in less than 230 years, the US did GAIN it's independence, survive a brief civil war, establish a free, and hopefully, equal society, spearhead many of the industrial revolution ideas (That cotton gin thing really had the world stumped, didn't it?) and create the most sophisticated military the planet has ever known. Yes, we do have our problems...The elderly, poor, and many children do not have access to adequate health care. 3) I do believe that Diana Goodwin stated, and I quote, "Happy Independence Day to all my fellow Americans!" Why respond at all if it doesn't apply to you? 4) Let all of my fellow Americans remember ALL the soldiers this weekend that are currently scattered throughout the world. Many are in places and battles that we will never know about. Climbs down off his soapbox, gets some BBQ to eat, and is ready for fireworks. Dennis Hahn dennish@cookchildrens.org >>> Kemlo Rogerson 6/30/2006 8:52 AM >>> Question? Can we say 'Happy Independence Day'? It was from us you were made independent from, so should we be sad? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 Distance is meant to relate, not separate. --Satish Kumar This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------ ------------------------------------ Cook Children's Health Care System This e-mail, facsimile, or letter and any files or attachments transmitted may contain information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Cook Children's Health Care System immediately at (682)885-4000 or via e-mail at compliance@cookchildrens.org. Cook Children's Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. ------------------------------------------------------------------------ ----------------------------------- The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 16 Date: Fri, 30 Jun 2006 12:47:47 -0400 From: "Michael Fredrickson" Subject: [Histonet] PCR for Mycobacteria on FFPE To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" We are trying to locate a commercial / clinical laboratory that performs PCR on formalin-fixed paraffin embedding tissue for Mycobacteria. Any help would be appreciated. Thanks. Michael M. Fredrickson mfredrickson@cohenderm.com ------------------------------ Message: 17 Date: Fri, 30 Jun 2006 12:49:23 -0400 From: Timothy Macatee Subject: Re: [Histonet] Tissue-Tek Express To: "Bartlett, Jeanine (CDC/NCID/VR)" , "Goodwin, Diana" , Dennis Hahn , HistoNet@Pathology.swmed.edu, kemlo.rogerson@waht.swest.nhs.uk Message-ID: Content-Type: text/plain; charset=US-ASCII Just for anyone who is interested, I found this web site on the American Flag a nice overview. Happy Independent 4th. http://www.ushistory.org/betsy/flagetiq.html#1 Tim On 6/30/06 12:24 PM, "Bartlett, Jeanine (CDC/NCID/VR)" wrote: > Is it okay that I am GOP but don't have a gun? > > > Jeanine Bartlett, BS, HT(ASCP) > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goodwin, > Diana > Sent: Friday, June 30, 2006 12:08 PM > To: Dennis Hahn; HistoNet@Pathology.swmed.edu; > kemlo.rogerson@waht.swest.nhs.uk > Subject: RE: [Histonet] Tissue-Tek Express > > Here, Here, and a resounding "Huzzah!" So nice that we bleeding-heart > liberals can wholeheartedly agree and identify with our gun-toting GOP > brethren now and again. > > Now I'm going home to hoist an ice-cold Coors and rent "The Patriot". > > Best Regards to ALL, > Diana Goodwin > > ________________________________ > > From: Dennis Hahn [mailto:DennisH@cookchildrens.org] > Sent: Friday, June 30, 2006 11:56 AM > To: Goodwin, Diana; HistoNet@Pathology.swmed.edu; > kemlo.rogerson@waht.swest.nhs.uk > Subject: RE: [Histonet] Tissue-Tek Express > > > Histo Net, > > Climbs upon his American, gun-toting soap-box.... > > Let the Friday flaming begin. This is not meant to offend any one > nationality. I usually just pick up tid-bits of information from the > histonet, and ignore all the usual comments or flamings. > > However, maybe the wording was not meant to be what I interpret it as, > but... > > 1) We were not "made" independent from anyone. No other country, or > king, gave us our independence. We fought for it. I served in our armed > forces for 6 years active duty, including the "first" gulf "conflict" as > it is referred to. I fly an American flag in my front yard and do my > best to explain to my children each time we take it down and burn it > (Yes, you SHOULD burn the American flag when it is worn out, and no > longer usable...it represents our country, a living, breathing entity) > that many men, and women, have died so that the people in our nation, > and others, may vote for our representatives both locally and > nationally, say what we want, when we want, come and go throughout our > country and the world as we please, all without the fear of any > retaliation when we lay our heads down at night to sleep because of it. > > 2) I'm not saying that anyone should be sad, but in less than 230 years, > the US did GAIN it's independence, survive a brief civil war, establish > a free, and hopefully, equal society, spearhead many of the industrial > revolution ideas (That cotton gin thing really had the world stumped, > didn't it?) and create the most sophisticated military the planet has > ever known. Yes, we do have our problems...The elderly, poor, and many > children do not have access to adequate health care. > > 3) I do believe that Diana Goodwin stated, and I quote, "Happy > Independence Day to all my fellow Americans!" Why respond at all if it > doesn't apply to you? > > 4) Let all of my fellow Americans remember ALL the soldiers this weekend > that are currently scattered throughout the world. Many are in places > and battles that we will never know about. > > Climbs down off his soapbox, gets some BBQ to eat, and is ready for > fireworks. > > Dennis Hahn > dennish@cookchildrens.org > > >>>> Kemlo Rogerson 6/30/2006 8:52 AM >>>> > > Question? Can we say 'Happy Independence Day'? It was from us you were > made > independent from, so should we be sad? > > Kemlo Rogerson > Pathology Manager > Ext 3311 > DD 01934 647057 > Mob 07749 754194 > Pager 07659 597107 > > Distance is meant to relate, not separate. --Satish Kumar > > > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on > its > contents: to do so is strictly prohibited and may be unlawful. Please > inform > me that this message has gone astray before deleting it. Thank you for > your > co-operation > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------------------------------------------------ > ------------------------------------ > Cook Children's Health Care System > > This e-mail, facsimile, or letter and any files or attachments > transmitted may contain information that is confidential and privileged. > This information is intended only for the use of the individual(s) and > entity(ies) to whom it is addressed. If you are the intended recipient, > further disclosures are prohibited without proper authorization. If you > are not the intended recipient, any disclosure, copying, printing, or > use of this information is strictly prohibited and possibly a violation > of federal or state law and regulations. > > If you have received this information in error, please notify Cook > Children's Health Care System immediately at (682)885-4000 or via e-mail > at compliance@cookchildrens.org. Cook Children's Health Care System, its > subsidiaries, and affiliates hereby claim all applicable privileges > related to this information. > ------------------------------------------------------------------------ > ----------------------------------- > > > The information contained in this e-mail message is intended only for > the personal and confidential use of the recipient(s) named above. If > the reader of this message is not the intended recipient or an agent > responsible for delivering it to the intended recipient, you are hereby > notified that you have received this document in error and that any > review, dissemination, distribution, or copying of this message is > strictly prohibited. If you have received this communication in error, > please notify us immediately by e-mail, and delete the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Tim Macatee Research Histology Core New York University School of Medicine 550 First Ave. Department of Pathology. Medical Science Building - Room 521 New York, N.Y. 10016 (212) 263-3888 ------------------------------ Message: 18 Date: Fri, 30 Jun 2006 12:57:04 -0400 From: "Joyce Cline" Subject: [Histonet] RE: Formula 83 To: Message-ID: <000601c69c66$371ccf30$1d2a14ac@wchsys.org> Content-Type: text/plain;charset="US-ASCII" I use Formula 83 in my processor, staining and coverslipping of H&E and IHC. I find it works well, better than the former substitute I was using. Also, dries real quickly and recycles well. ------------------------------------------------------------------------ ---- Has anyone tried this product? It is produced by CBG Biotech. Thanks and Happy 4th of July to those who ware taking a nice long weekend. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 31, Issue 43 **************************************** From lrichey <@t> u.washington.edu Fri Jun 30 13:27:51 2006 From: lrichey <@t> u.washington.edu (Lori Richey) Date: Fri Jun 30 13:28:06 2006 Subject: [Histonet] Feedback Re: Tissue-Tek Express Instrument In-Reply-To: <80CDD9C3FEEAFD4982B114C4A6DFD00E02CB7F21@uphsmbx2.UPHS.PENNHEALTH.PRV> References: <80CDD9C3FEEAFD4982B114C4A6DFD00E02CB7F21@uphsmbx2.UPHS.PENNHEALTH.PRV> Message-ID: <44A56D27.4010902@u.washington.edu> The Sakura Express processor works well, for rapid processing during the day of rush liver, cardiac, renal, and prostate biopsies. We do not use it for routine surgical, skin or breast biopsies. Use of it depends on the pathologists reading the slides. The H&E staining is different, more brilliant in color. Goodwin, Diana wrote: >Since my original post was turned into a political debate, I will try >again to solicit the information that I am interested in obtaining: > >Is anyone out there using this instrument, and if so, what are your >thoughts on the return on investment: functionality, cost savings, >quality compared to overnight, any savings in tech-time, etc. > >Thanks again, >Diana Goodwin >Supervisor, Anatomic Pathology >Pennsylvania Hospital >Preston 655-C >ph. 215-829-6532 >pager 215-422-5160 >fax 215-829-7564 >e-mail goodwind@[pahosp.com > > > >The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Rcartun <@t> harthosp.org Fri Jun 30 13:45:34 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Jun 30 13:46:12 2006 Subject: [Histonet] PCR for Mycobacteria on FFPE In-Reply-To: References: Message-ID: <44A5390E0200007700000BFF@hcnwgwds01.hh.chs> Try Dr. Margie Scott's Lab in Little Rock, AR. Their telephone number is (501) 257-6433. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Michael Fredrickson" 06/30/06 12:47 PM >>> We are trying to locate a commercial / clinical laboratory that performs PCR on formalin-fixed paraffin embedding tissue for Mycobacteria. Any help would be appreciated. Thanks. Michael M. Fredrickson mfredrickson@cohenderm.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Eric <@t> ategra.com Thu Jun 29 15:09:04 2006 From: Eric <@t> ategra.com (Eric Dye (ext 223)) Date: Fri Jun 30 14:02:07 2006 Subject: [Histonet] Histology Jobs in your area Message-ID: Hi - Fellow-Histonetters Below is the updated list of Histology j o b s, All openings are Dayshift Monday thru Friday unless indicated otherwise. All of these jobs are looking to move quickly so if your interested call me A S A P... If you are interested in any of the Histology jobs listed below or anywhere else - please call me at 800-466-9919 ext 223 or cell - 407-756-5507. Most HistoTech j o b s are permanent full-time Here are some of my Newest Histology Jobs: ------------------------------------------------------ New York (Long Island) - HistoTech - perm Northern New Jersey - HistoTech and Cyto Tech - perm Boston Mass - HistoTech - one Senior HistoTech, One not so Senior Histotech Massachusetts (North of Boston) - perm - Bench Histotech Ohio (Central) - one temp and one perm - Bench Histotech West Virginia - perm - Bench Histotech/ Supervisor Minnesota (Twin Cities area - perm - Bench Histotech - Biotech/research - Make your own schedule!!! Southeast Florida - temp - HistoTech Southwest Florida - perm - Bench Histotech/ Supervisor Florida, West Coast - both temp & perm openings- Bench Histotech Pennsylvania, Pittsburgh - perm - Bench HistoTech Virginia/D.C. Beltway East - Fairfax Area - Perm, HistoTech / Histo Supervisor New Hampshire - - both temp & perm openings - Histotech South Carolina - Perm - Supervisor and Bench - Histotech Rhode Island - perm - Histotech ---- end list of HistoTech Oppurtunties --- If you are interested in any of the Histology jobs listed above - please call me at 800-466-9919 ext 223 or cell - 407-756-5507. Thank you, Eric Dye-Sr Allied Healthcare Recruiter 800-466-9919 ext 223 or Cell - 407-756-5507 P.S.: Feel free to pass along this email and my phone number to anyone who you think might be interested. P.S.S.: The clients are currently interviewing - and the job will close soon - so if you are interested, please call me today at 1-800-466-9919 ext 229 --------------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing 800 466 9919 ext 223 - voice 407 671 6075 - fax [1]eric@ategra.com To Learn More About Ategra: [2]http://www.ategra.com ---------------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. ---------------------------------------------------------------- ---------------------------------------------------------------- Note: this message is intended for: Fellow-Histonetters at histonet@lists.utsouthwestern.edu ---------------------------------------------------------------- References 1. mailto:eric@ategra.com 2. http://www.ategra.com/ From sbreeden <@t> nmda.nmsu.edu Fri Jun 30 14:04:19 2006 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Jun 30 14:04:24 2006 Subject: [Histonet] FRIDAY Musing Message-ID: Picture this: NSH is in Phoenix - the home of the AZ Diamondbacks - this September. Cowabunga! - the Diamondbacks are at home that week (Sep 7-13) playing St. Louis and then Washington. I'm a baseball fan and I'm thinkin' there are other baseball fans who happen to be histotechs who might be interested in getting together to go to a game! We could call it Histonight at Chase Field. I'm sure there's someone in PHX that could get a group of tickets... What say you, fellow(ette) baseball fan? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87108 From Janet.Bonner <@t> FLHOSP.ORG Fri Jun 30 14:38:40 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Fri Jun 30 14:40:10 2006 Subject: [Histonet] Feedback Re: Tissue-Tek Express Instrument References: <80CDD9C3FEEAFD4982B114C4A6DFD00E02CB7F21@uphsmbx2.UPHS.PENNHEALTH.PRV> Message-ID: Better to ask serious question on Monday....( Have a great Fourth of July!!!!) @:) ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Goodwin, Diana Sent: Fri 6/30/2006 1:13 PM To: HistoNet@Pathology.swmed.edu Subject: [Histonet] Feedback Re: Tissue-Tek Express Instrument Since my original post was turned into a political debate, I will try again to solicit the information that I am interested in obtaining: Is anyone out there using this instrument, and if so, what are your thoughts on the return on investment: functionality, cost savings, quality compared to overnight, any savings in tech-time, etc. Thanks again, Diana Goodwin Supervisor, Anatomic Pathology Pennsylvania Hospital Preston 655-C ph. 215-829-6532 pager 215-422-5160 fax 215-829-7564 e-mail goodwind@[pahosp.com The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dcd710 <@t> charter.net Fri Jun 30 15:15:07 2006 From: dcd710 <@t> charter.net (dcd710@charter.net) Date: Fri Jun 30 15:15:12 2006 Subject: [Histonet] Formula 83 Message-ID: <1923452717.1151698507106.JavaMail.root@fepweb06> ---- "Molinari wrote: > Happy Friday. > > Has anyone tried this product? It is produced by CBG Biotech. > > Thanks and Happy 4th of July to those who ware taking a nice long > weekend. > > > We have used Formula 83 for almost 2 years and have had absolutely NO PROBLEMS with it. It's about 1/2 the cost of the xylene we were purchasing and the health hazards are much lower. Also, we have a CBG Biotech recycler and get great results from this instrument. If you'd like more information, contact me direct. DDietz > Betsy Molinari HT (ASCP) > > Texas Heart Institute > > Cardiovascular Pathology > > 6770 Bertner Ave. > > Houston,TX 77030 > > 832-355-6524 > > 832-355-6812 (fax) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From crains <@t> wpmpath.com Fri Jun 30 15:19:26 2006 From: crains <@t> wpmpath.com (crains@wpmpath.com) Date: Fri Jun 30 15:19:33 2006 Subject: [Histonet] Feedback Re: Tissue-Tek Express Instrument Message-ID: <20060630201927.RVJX3326.dukecmmtao03.coxmail.com@dukecmmtao03> can I go home now? > > From: "Bonner, Janet" > Date: 2006/06/30 Fri PM 02:38:40 CDT > To: "Goodwin, Diana" , HistoNet@Pathology.swmed.edu > Subject: RE: [Histonet] Feedback Re: Tissue-Tek Express Instrument > > Better to ask serious question on Monday....( Have a great Fourth of July!!!!) @:) > > ________________________________ > > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Goodwin, Diana > Sent: Fri 6/30/2006 1:13 PM > To: HistoNet@Pathology.swmed.edu > Subject: [Histonet] Feedback Re: Tissue-Tek Express Instrument > > > > Since my original post was turned into a political debate, I will try > again to solicit the information that I am interested in obtaining: > > Is anyone out there using this instrument, and if so, what are your > thoughts on the return on investment: functionality, cost savings, > quality compared to overnight, any savings in tech-time, etc. > > Thanks again, > Diana Goodwin > Supervisor, Anatomic Pathology > Pennsylvania Hospital > Preston 655-C > ph. 215-829-6532 > pager 215-422-5160 > fax 215-829-7564 > e-mail goodwind@[pahosp.com > > > > The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Important: This email and any attachments may contain confidential information subject to protection under the Federal Standards for Privacy of Individually Identifiable Health Information (45 C.F.R. Parts 160 and 164). If you or your organization is a "Covered Entity" under the above mentioned regulations, you are obligated to treat such information in a manner consistent with the regulations. If it appears that this email was sent to you in error, (1) you are prohibited from utilizing or disseminating this email or any attachments; (2) please immediately delete it from your computer and any servers or other locations where it might be stored and email (crains@wpmpath.com) or call (Chris Rains) at 785-823-7201 advising that you have done so. We appreciate your cooperation. From rodger_65489 <@t> yahoo.com Fri Jun 30 15:42:28 2006 From: rodger_65489 <@t> yahoo.com (Roger Smithwell) Date: Fri Jun 30 15:42:32 2006 Subject: [Histonet] Digital imaging system Message-ID: <20060630204229.96030.qmail@web31404.mail.mud.yahoo.com> HistoNetters, Anyone out there in "histo land" have experience or looking for a digital imaging system for your specimen? Any info would be generous! Thanks ! --------------------------------- How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. From liz <@t> premierlab.com Fri Jun 30 16:18:44 2006 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Jun 30 16:18:53 2006 Subject: [Histonet] Digital imaging system In-Reply-To: <20060630204229.96030.qmail@web31404.mail.mud.yahoo.com> Message-ID: <000701c69c8a$c49cfb40$0300a8c0@Chlipala> Roger We have been looking at several whole slides scanning systems, but have not purchased one yet, they are somewhat pricy ranging from $80,000.00 up. I'm aware of several Aperio - I have worked with this unit and its nice, it scans in strips rather than tiles like most units currently on the market Dako - scans in tiles, but I think that it has more analysis software built in Chromovision - Applied Imaging D.metrix - new technology that uses an array of 80 lenses to scan the image. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roger Smithwell Sent: Friday, June 30, 2006 2:42 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Digital imaging system HistoNetters, Anyone out there in "histo land" have experience or looking for a digital imaging system for your specimen? Any info would be generous! Thanks ! --------------------------------- How low will we go? Check out Yahoo! Messenger's low PC-to-Phone call rates. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet