From tinayates <@t> comcast.net Sat Jul 1 00:30:05 2006 From: tinayates <@t> comcast.net (Comcast Mail) Date: Sat Jul 1 00:30:13 2006 Subject: [Histonet] HISTOLOGY JOB OPENING Message-ID: <040d01c69ccf$68f9c300$05f8c147@DFC4J821> www.salemhospital.org Company: Salem Hospital Contact: Cyndi Walling Phone: 503-561-5183 WORK DAYS: Monday-Friday with rotating Saturdays We are currently recruiting for the following positions: Histologist - Lab Pathology (#3449) FT-40 0400-1230 (8/40) Histologist - Lab Pathology (#3393) FT-40 0400-1230 (8/40) If you are looking for a beautiful place to work and play, then take a look at Salem Hospital. We are located in the capital of Oregon, which is in the heart of the Willamette Valley. Only one hour separates you from calm ocean beaches and snow covered mountains. We look forward to welcoming you to our team at Salem Hospital! As a Histologist, you will perform established histology procedures under general supervision and may also demonstrate histology procedures to other employees; is generally knowledgeable and skilled in all disciplines of histology medicine. The Histologist prepares patient samples for histology testing using cutting and embedding equipment, and also performs staining procedures. This position is responsible for the accurate performance of tests, use of controls to assure accuracy of test results and the exercise of judgment in the case of questionable or abnormal results. Job involves exposure to many different types of infectious materials, as well as infectious people. Position comes into contact with a variety of hazardous substances, including chemicals and low-level radioactive material. Job Requirements: -Graduation from a recognized program of Histotechnology or the equivalent and minimum certification as HT (ASCP). In the case of equivalence, competency in the field must be established. New graduates from a program of Histotechnology may be considered, but must complete ASCP Certification within 12 months of hire. From shawnster73 <@t> aol.com Sat Jul 1 09:01:05 2006 From: shawnster73 <@t> aol.com (shawnster73@aol.com) Date: Sat Jul 1 09:01:27 2006 Subject: [Histonet] Formula 83 In-Reply-To: <1923452717.1151698507106.JavaMail.root@fepweb06> References: <1923452717.1151698507106.JavaMail.root@fepweb06> Message-ID: <8C86B2DEDD01E48-18E4-335@mblk-d51.sysops.aol.com> I am also just trying Formula 83. Has anyone used it on animal tissues? -----Original Message----- From: dcd710@charter.net To: Molinari, Betsy ; histonet@lists.utsouthwestern.edu Sent: Fri, 30 Jun 2006 13:15:07 -0700 Subject: Re: [Histonet] Formula 83 ---- "Molinari wrote: > Happy Friday. > > Has anyone tried this product? It is produced by CBG Biotech. > > Thanks and Happy 4th of July to those who ware taking a nice long > weekend. > > > We have used Formula 83 for almost 2 years and have had absolutely NO PROBLEMS with it. It's about 1/2 the cost of the xylene we were purchasing and the health hazards are much lower. Also, we have a CBG Biotech recycler and get great results from this instrument. If you'd like more information, contact me direct. DDietz > Betsy Molinari HT (ASCP) > > Texas Heart Institute > > Cardiovascular Pathology > > 6770 Bertner Ave. > > Houston,TX 77030 > > 832-355-6524 > > 832-355-6812 (fax) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Check out AOL.com today. Breaking news, video search, pictures, email and IM. All on demand. Always Free. From H.I.Grabsch <@t> leeds.ac.uk Sat Jul 1 14:19:02 2006 From: H.I.Grabsch <@t> leeds.ac.uk (Heike Grabsch) Date: Sat Jul 1 14:23:08 2006 Subject: [Histonet] digital imaging References: <200607011709.k61H9ZQT003518@mserv1.leeds.ac.uk> Message-ID: We use the Aperio system extensively, we have 5 of them and have scan thousands and thousands of pictures as all the colorectal cancer clinical trial slides are scanned. We use it also for routine remote reporting in Neuropathology and scanning for research, education etc. You can see the public side of what we do under http://www.virtualpathology.leeds.ac.uk/ What do you want to do with it? My collaborators in Amsterdam have the system from Zeiss as it can also scans fluorescent stainings. If you are interested in that system, I could give you their email. Heike Dr Heike Grabsch, MRCPath MD Gastrointestinal Cancer Research Group The Leeds Institute of Molecular Medicine Section of Pathology and Tumour Biology Wellcome Trust Brenner Bldg, Level 4 St James's University Hospital Beckett Street Leeds LS9 7TF ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of histonet-request@lists.utsouthwestern.edu Sent: Sat 01/07/2006 18:09 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 32, Issue 1 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Digital imaging system (Roger Smithwell) 2. RE: Digital imaging system (Elizabeth Chlipala) 3. HISTOLOGY JOB OPENING (Comcast Mail) 4. Re: Formula 83 (shawnster73@aol.com) ---------------------------------------------------------------------- Message: 1 Date: Fri, 30 Jun 2006 13:42:28 -0700 (PDT) From: Roger Smithwell Subject: [Histonet] Digital imaging system To: Histonet@lists.utsouthwestern.edu Message-ID: <20060630204229.96030.qmail@web31404.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 HistoNetters, Anyone out there in "histo land" have experience or looking for a digital imaging system for your specimen? Any info would be generous! Thanks ! --------------------------------- How low will we go? Check out Yahoo! Messenger's low PC-to-Phone call rates. ------------------------------ Message: 2 Date: Fri, 30 Jun 2006 15:18:44 -0600 From: "Elizabeth Chlipala" Subject: RE: [Histonet] Digital imaging system To: "'Roger Smithwell'" , Message-ID: <000701c69c8a$c49cfb40$0300a8c0@Chlipala> Content-Type: text/plain; charset="us-ascii" Roger We have been looking at several whole slides scanning systems, but have not purchased one yet, they are somewhat pricy ranging from $80,000.00 up. I'm aware of several Aperio - I have worked with this unit and its nice, it scans in strips rather than tiles like most units currently on the market Dako - scans in tiles, but I think that it has more analysis software built in Chromovision - Applied Imaging D.metrix - new technology that uses an array of 80 lenses to scan the image. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roger Smithwell Sent: Friday, June 30, 2006 2:42 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Digital imaging system HistoNetters, Anyone out there in "histo land" have experience or looking for a digital imaging system for your specimen? Any info would be generous! Thanks ! --------------------------------- How low will we go? Check out Yahoo! Messenger's low PC-to-Phone call rates. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Fri, 30 Jun 2006 22:30:05 -0700 From: "Comcast Mail" Subject: [Histonet] HISTOLOGY JOB OPENING To: Message-ID: <040d01c69ccf$68f9c300$05f8c147@DFC4J821> Content-Type: text/plain; charset="iso-8859-1" www.salemhospital.org Company: Salem Hospital Contact: Cyndi Walling Phone: 503-561-5183 WORK DAYS: Monday-Friday with rotating Saturdays We are currently recruiting for the following positions: Histologist - Lab Pathology (#3449) FT-40 0400-1230 (8/40) Histologist - Lab Pathology (#3393) FT-40 0400-1230 (8/40) If you are looking for a beautiful place to work and play, then take a look at Salem Hospital. We are located in the capital of Oregon, which is in the heart of the Willamette Valley. Only one hour separates you from calm ocean beaches and snow covered mountains. We look forward to welcoming you to our team at Salem Hospital! As a Histologist, you will perform established histology procedures under general supervision and may also demonstrate histology procedures to other employees; is generally knowledgeable and skilled in all disciplines of histology medicine. The Histologist prepares patient samples for histology testing using cutting and embedding equipment, and also performs staining procedures. This position is responsible for the accurate performance of tests, use of controls to assure accuracy of test results and the exercise of judgment in the case of questionable or abnormal results. Job involves exposure to many different types of infectious materials, as well as infectious people. Position comes into contact with a variety of hazardous substances, including chemicals and low-level radioactive material. Job Requirements: -Graduation from a recognized program of Histotechnology or the equivalent and minimum certification as HT (ASCP). In the case of equivalence, competency in the field must be established. New graduates from a program of Histotechnology may be considered, but must complete ASCP Certification within 12 months of hire. ------------------------------ Message: 4 Date: Sat, 01 Jul 2006 10:01:05 -0400 From: shawnster73@aol.com Subject: Re: [Histonet] Formula 83 To: Histonet@lists.utsouthwestern.edu Message-ID: <8C86B2DEDD01E48-18E4-335@mblk-d51.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" I am also just trying Formula 83. Has anyone used it on animal tissues? -----Original Message----- From: dcd710@charter.net To: Molinari, Betsy ; histonet@lists.utsouthwestern.edu Sent: Fri, 30 Jun 2006 13:15:07 -0700 Subject: Re: [Histonet] Formula 83 ---- "Molinari wrote: > Happy Friday. > > Has anyone tried this product? It is produced by CBG Biotech. > > Thanks and Happy 4th of July to those who ware taking a nice long > weekend. > > > We have used Formula 83 for almost 2 years and have had absolutely NO PROBLEMS with it. It's about 1/2 the cost of the xylene we were purchasing and the health hazards are much lower. Also, we have a CBG Biotech recycler and get great results from this instrument. If you'd like more information, contact me direct. DDietz > Betsy Molinari HT (ASCP) > > Texas Heart Institute > > Cardiovascular Pathology > > 6770 Bertner Ave. > > Houston,TX 77030 > > 832-355-6524 > > 832-355-6812 (fax) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Check out AOL.com today. Breaking news, video search, pictures, email and IM. All on demand. Always Free. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 32, Issue 1 *************************************** From yeepengtiang <@t> hotmail.com Sat Jul 1 21:55:13 2006 From: yeepengtiang <@t> hotmail.com (tiang yeepeng) Date: Sat Jul 1 21:55:19 2006 Subject: [Histonet] PCR for Mycobacteria on FFPE Message-ID: Hi, I am a postgrad student from Malaysia. I do PCR for TB in clinical samples as routine. Sometime we receive FFPE samples. How may I help you? With regards, TIANG YEE PENG Department of Medical Microbiology, Faculty of Medicine, University of Malaya. _________________________________________________________________ Add photos, news, and blogs about the World Cup to your Live.com homepage! http://www.live.com/getstarted From nfournier <@t> sasktel.net Sat Jul 1 22:44:43 2006 From: nfournier <@t> sasktel.net (Neil Fournier) Date: Sat Jul 1 22:44:52 2006 Subject: [Histonet] nickel enhanced DAB procedure for rat brain Message-ID: <000801c69d89$db65a570$44ec6e40@NEIL6FC9056E3D> I was wondering if anyone has experience using nickel enhanced DAB? One of our protocols suggested using nickel enhanced DAB. We use 10 mg DAB tablets available from Sigma Chemicals to make up our DAB substrate solution. We dissolve our DAB in .1 M phosphate buffer (pH 7.4). Does anyone have suggestions for making up a nickel-DAB solution. If so, what is the typical concentration, recipe, and procedures for making up this solution? I also realize that some individuals dissolve the nickel ammonium sulphate in acetate buffer. Is this necessary? And if so, what is the rationale for this. Appreciate the advice, Neil From rjbuesa <@t> yahoo.com Sun Jul 2 08:47:45 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Jul 2 08:47:49 2006 Subject: [Histonet] nickel enhanced DAB procedure for rat brain In-Reply-To: <000801c69d89$db65a570$44ec6e40@NEIL6FC9056E3D> Message-ID: <20060702134745.75896.qmail@web61222.mail.yahoo.com> Neil: Nickel "enhancement" (a name used to develop a blue-purplish chromogen colour) uses a 16.2% aq.sol. of nickel sulfate hexahydrated in the following proportion: a- DAB ---- 6 mg b- PBS ---- 10 mL c- 3% hydrogen peroxide --- 160?L d- 16.2% nickel sulfate aq.sol. ---- 120 ?L Adjust the recipe for your 10 mg DAB tablets. Hope this will help you. Ren? J. Neil Fournier wrote: I was wondering if anyone has experience using nickel enhanced DAB? One of our protocols suggested using nickel enhanced DAB. We use 10 mg DAB tablets available from Sigma Chemicals to make up our DAB substrate solution. We dissolve our DAB in .1 M phosphate buffer (pH 7.4). Does anyone have suggestions for making up a nickel-DAB solution. If so, what is the typical concentration, recipe, and procedures for making up this solution? I also realize that some individuals dissolve the nickel ammonium sulphate in acetate buffer. Is this necessary? And if so, what is the rationale for this. Appreciate the advice, Neil _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Want to be your own boss? Learn how on Yahoo! Small Business. From beingmary53 <@t> sbcglobal.net Sun Jul 2 10:25:33 2006 From: beingmary53 <@t> sbcglobal.net (MARY JOHNSON) Date: Sun Jul 2 10:25:39 2006 Subject: [Histonet] job description Message-ID: <20060702152533.78571.qmail@web81604.mail.mud.yahoo.com> Hi Histonettes, I hope someone can help We are going to get a lab assistants and I am in need of a Job Description Hope someone can help. Please fax to 713/413/2253 Thank's Mary Johnson HT ascp From kemlo.rogerson <@t> waht.swest.nhs.uk Mon Jul 3 02:02:52 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Jul 3 02:02:16 2006 Subject: [Histonet] FRIDAY Musing Message-ID: What is base ball? I know Rugby, I know Football (Soccer), I know American Football (Rugby with armour on) and I even know Australian Football (American Football without the armour) and even that thing they do it Ireland but I don't know its name; is Base Ball like 'Rounders'? If so that's a spiffing game, old chap, and I'd love to see a game; mostly the ladies play it here. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 Distance is meant to relate, not separate. --Satish Kumar This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From kemlo.rogerson <@t> waht.swest.nhs.uk Mon Jul 3 02:13:24 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Jul 3 02:12:46 2006 Subject: [Histonet] Tissue-Tek Express Message-ID: Took longer than I expected and the response was minimal; only found one with a challenged 'sense of humour'. Happy Independence Day; really, for all those things you list and much, much more including your help in the last falling out we had with Germany. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 Distance is meant to relate, not separate. --Satish Kumar This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From AnthonyH <@t> chw.edu.au Mon Jul 3 02:30:17 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Jul 3 02:30:25 2006 Subject: [Histonet] FRIDAY Musing Message-ID: Look out, the sparkes will start to fly!! Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: Monday, 3 July 2006 5:03 PM To: 'Breeden, Sara'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] FRIDAY Musing What is base ball? I know Rugby, I know Football (Soccer), I know American Football (Rugby with armour on) and I even know Australian Football (American Football without the armour) and even that thing they do it Ireland but I don't know its name; is Base Ball like 'Rounders'? If so that's a spiffing game, old chap, and I'd love to see a game; mostly the ladies play it here. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 Distance is meant to relate, not separate. --Satish Kumar This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From cpomajzl <@t> cpllabs.com Mon Jul 3 08:35:38 2006 From: cpomajzl <@t> cpllabs.com (Chris Pomajzl) Date: Mon Jul 3 08:30:36 2006 Subject: [Histonet] FRIDAY Musing References: Message-ID: <002601c69ea5$93cd5e20$26fca8c0@CSP> Then we can assume that all of your ladies are 6'4" and weigh 240 lbs. (109kg for the Brits) ; ) Ha! Baseball = Uncivilized Cricket ----- Original Message ----- From: "Kemlo Rogerson" To: "'Breeden, Sara'" ; Sent: Monday, July 03, 2006 2:02 AM Subject: RE: [Histonet] FRIDAY Musing > What is base ball? I know Rugby, I know Football (Soccer), I know American > Football (Rugby with armour on) and I even know Australian Football > (American Football without the armour) and even that thing they do it > Ireland but I don't know its name; is Base Ball like 'Rounders'? If so > that's a spiffing game, old chap, and I'd love to see a game; mostly the > ladies play it here. > > Kemlo Rogerson > Pathology Manager > Ext 3311 > DD 01934 647057 > Mob 07749 754194 > Pager 07659 597107 > > Distance is meant to relate, not separate. --Satish Kumar > > > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on its > contents: to do so is strictly prohibited and may be unlawful. Please inform > me that this message has gone astray before deleting it. Thank you for your > co-operation > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Margaret.Perry <@t> sdstate.edu Mon Jul 3 08:31:17 2006 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Mon Jul 3 08:31:26 2006 Subject: [Histonet] RE: Formula 83 In-Reply-To: <20060630170117.4DFAE14E197@barracuda.sdstate.edu> Message-ID: We are a veterinary diagnostic lab and have been using formula 83 for several years. It works well on H&E, special stains and IHC. Margaret Perry HT(ASCP) Animal Disease Research and Diagnostic Lab South Dakota State University Brookings SD Margaret.perry@sdstate.edu From Reuel.Cornelia <@t> tsrh.org Mon Jul 3 08:37:13 2006 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Mon Jul 3 08:39:50 2006 Subject: [Histonet] Powerpath Message-ID: Just wanted to Thank those who responded on my question about powerpath. I have it sorted out. Thank you very much. Reuel ******************************************************************************************************************* Texas Scottish Rite Hospital for Children is one of the nation's leading pediatric centers for the treatment of orthopedic conditions and learning disorders, like dyslexia. This email transmission and/or its attachments may contain confidential health information, intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing it to any other party unless required to do so by law and is required to delete/destroy the information after its stated need has been fulfilled. If you are not the intended recipient, any disclosure, copying, distribution or action taken in reliance on the contents of this email transmission is prohibited. If you have received this information in error, please notify the sender immediately and delete this information. We appreciate your efforts to protect the children's confidential information. ******************************************************************************************************************* From Barry.R.Rittman <@t> uth.tmc.edu Mon Jul 3 08:38:53 2006 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Mon Jul 3 08:40:15 2006 Subject: [Histonet] FRIDAY Musing Message-ID: Speaking as someone born in Britain but who has lived in America for 38 years...Baseball is much more exciting than cricket. Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Chris Pomajzl Sent: Mon 7/3/2006 8:35 AM To: HISTONET Subject: Re: [Histonet] FRIDAY Musing Then we can assume that all of your ladies are 6'4" and weigh 240 lbs. (109kg for the Brits) ; ) Ha! Baseball = Uncivilized Cricket ----- Original Message ----- From: "Kemlo Rogerson" To: "'Breeden, Sara'" ; Sent: Monday, July 03, 2006 2:02 AM Subject: RE: [Histonet] FRIDAY Musing > What is base ball? I know Rugby, I know Football (Soccer), I know American > Football (Rugby with armour on) and I even know Australian Football > (American Football without the armour) and even that thing they do it > Ireland but I don't know its name; is Base Ball like 'Rounders'? If so > that's a spiffing game, old chap, and I'd love to see a game; mostly the > ladies play it here. > > Kemlo Rogerson > Pathology Manager > Ext 3311 > DD 01934 647057 > Mob 07749 754194 > Pager 07659 597107 > > Distance is meant to relate, not separate. --Satish Kumar > > > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on its > contents: to do so is strictly prohibited and may be unlawful. Please inform > me that this message has gone astray before deleting it. Thank you for your > co-operation > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Mon Jul 3 09:13:47 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Mon Jul 3 09:12:06 2006 Subject: [Histonet] FRIDAY Musing Message-ID: Since I first saw baseball some 50 years ago, I've trying to catch site of the wicket. Another dismal failure. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Chris Pomajzl [mailto:cpomajzl@cpllabs.com] Sent: 03 July 2006 14:36 To: HISTONET Subject: Re: [Histonet] FRIDAY Musing Then we can assume that all of your ladies are 6'4" and weigh 240 lbs. (109kg for the Brits) ; ) Ha! Baseball = Uncivilized Cricket ----- Original Message ----- From: "Kemlo Rogerson" To: "'Breeden, Sara'" ; Sent: Monday, July 03, 2006 2:02 AM Subject: RE: [Histonet] FRIDAY Musing > What is base ball? I know Rugby, I know Football (Soccer), I know American > Football (Rugby with armour on) and I even know Australian Football > (American Football without the armour) and even that thing they do it > Ireland but I don't know its name; is Base Ball like 'Rounders'? If so > that's a spiffing game, old chap, and I'd love to see a game; mostly the > ladies play it here. > > Kemlo Rogerson > Pathology Manager > Ext 3311 > DD 01934 647057 > Mob 07749 754194 > Pager 07659 597107 > > Distance is meant to relate, not separate. --Satish Kumar > > > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on its > contents: to do so is strictly prohibited and may be unlawful. Please inform > me that this message has gone astray before deleting it. Thank you for your > co-operation > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From algranth <@t> u.arizona.edu Mon Jul 3 10:05:55 2006 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Mon Jul 3 10:06:03 2006 Subject: [Histonet] FRIDAY Musing/baseball game In-Reply-To: Message-ID: <4.3.2.7.2.20060703074618.022bbb18@algranth.inbox.email.arizona.edu> Sara, Unfortunately the D'backs are playing crummy baseball right now and tickets are not hard to get. Unless they wake up and start playing ball in the second half of the season you won't have a hard time getting tickets. Chase Field is a very short walk from the hotels and you can go over there the morning of the game and get tickets. They have a TGI Fridays (called Fridays Front Row) where you can go for dinner before the game. If you want to spend more money you can reserve a table to sit at and watch the game while you munch. We like to go and sit at one of the outside tables for dinner and watch batting practice then go to our seats. You can buy your tickets online too: http://arizona.diamondbacks.mlb.com/NASApp/mlb/index.jsp?c_id=ari See you in Phoenix. Can you bring them a new pitcher or two? Have fun if you go! Andi Grantham At 01:04 PM 6/30/2006 -0600, Breeden, Sara wrote: >Picture this: NSH is in Phoenix - the home of the AZ Diamondbacks - this >September. Cowabunga! - the Diamondbacks are at home that week (Sep >7-13) playing St. Louis and then Washington. I'm a baseball fan and I'm >thinkin' there are other baseball fans who happen to be histotechs who >might be interested in getting together to go to a game! We could call >it Histonight at Chase Field. I'm sure there's someone in PHX that >could get a group of tickets... What say you, fellow(ette) baseball >fan? > > > >Sally Breeden, HT(ASCP) > >NM Dept. of Agriculture > >Veterinary Diagnostic Services > >PO Box 700 > >Albuquerque, NM 87108 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From Charles.Embrey <@t> carle.com Mon Jul 3 10:26:02 2006 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Mon Jul 3 10:26:09 2006 Subject: [Histonet] FRIDAY Musing Message-ID: More appropriately for Brits- 17.14 stone. Chuck -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Chris Pomajzl Sent: Monday, July 03, 2006 8:36 AM To: HISTONET Subject: Re: [Histonet] FRIDAY Musing Then we can assume that all of your ladies are 6'4" and weigh 240 lbs. (109kg for the Brits) ; ) Ha! Baseball = Uncivilized Cricket ----- Original Message ----- From: "Kemlo Rogerson" To: "'Breeden, Sara'" ; Sent: Monday, July 03, 2006 2:02 AM Subject: RE: [Histonet] FRIDAY Musing > What is base ball? I know Rugby, I know Football (Soccer), I know American > Football (Rugby with armour on) and I even know Australian Football > (American Football without the armour) and even that thing they do it > Ireland but I don't know its name; is Base Ball like 'Rounders'? If so > that's a spiffing game, old chap, and I'd love to see a game; mostly the > ladies play it here. > > Kemlo Rogerson > Pathology Manager > Ext 3311 > DD 01934 647057 > Mob 07749 754194 > Pager 07659 597107 > > Distance is meant to relate, not separate. --Satish Kumar > > > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on its > contents: to do so is strictly prohibited and may be unlawful. Please inform > me that this message has gone astray before deleting it. Thank you for your > co-operation > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From shive003 <@t> umn.edu Mon Jul 3 10:57:54 2006 From: shive003 <@t> umn.edu (Jan Shivers) Date: Mon Jul 3 10:58:28 2006 Subject: [Histonet] veterinary B cell IHC marker Message-ID: <01f601c69eb9$72761f00$a1065486@auxs.umn.edu> Hi all, Does anyone have a vendor recommendation for a B cell IHC marker (on FFPE tissue) which will work on non-human/primate mammalians? I had been using B Lymphocyte Marker by DAKO, but I've just been informed that they've discontinued that antibody. Needless to say, the absence of a B Cell antibody is a crisis for me. Thanks in advance. Jan Shivers Section Head Histo/IHC/EM UMN Vet Diag Lab From tkngflght <@t> yahoo.com Mon Jul 3 11:00:51 2006 From: tkngflght <@t> yahoo.com (Cheryl) Date: Mon Jul 3 11:00:57 2006 Subject: [Histonet] Hi Everyone--I need some staffing help-- In-Reply-To: Message-ID: <20060703160051.21083.qmail@web50904.mail.yahoo.com> Hello- I weigh in here every once in a while to ask questions for my bench work and help out my fellow-techs. Now I need a little help! I know there are folks here who don't think this should be used as a forum for soliciting business: I am soliciting employees--I have plenty of business--I need about 10 more techs! (registered or not--as long as you know your stuff!). I operate a small Histology staffing service--and the travel division is GROWING!!! How would you like to work in all sorts of places, learn lots of new things, earn a great wage with lots of perks and all for a company run by a tech who's walked in these shoes (since 1983) ? I welcome all questions: I'll give you references--talk to some of the people who travel for me and they'll tell you I keep it fun, I keep my word (I put everything in writing), and even if there are a few icky things, I'll tell you straight up. Not quite ready? Give me a call and I'll run through 'Travel Tech 101' so you can make an educated decision. It takes about 10 minutes, you'll learn a little about how traveling works and if you like--you can register so you'll get a call or email for new jobs you might enjoy. I won't try to shove you into a job that doesn't fit just because we have a contract to fill--no pressure, no hassles. We run a generous referral program--if you know people seeking permanent or who are curious about travel, call me and I'll explain how it works. I need unregistered and registered Histotechs, a few with State Licenses, and we have openings for MT/MLTs as well. How often do you run into a situation where EVERYONE wins--this is one. I appreciate this forum--I still work as a tech and you have helped me be better in my chosen field. Thanks for allowing my request!! PLEASE RESPOND TO THE EMAIL BELOW :) Cheryl Kerry, HT(ASCP) Full Staff Inc. admin@fullstaff.org www.fullstaff.org 800.756.3309 phone and fax Staffing the AP Lab by helping one tech at a time. Temporary and Permanent openings across all 50 States. From RSRICHMOND <@t> aol.com Mon Jul 3 12:20:55 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Mon Jul 3 12:21:01 2006 Subject: [Histonet] Re: FRIDAY musing Message-ID: <436.3cc83e5.31daabf7@aol.com> Kemlo wants to know - >>what is base ball?<< I think baseball and cricket evolved from the same schoolyard game, some time in the early 19th century. I watched a couple of cricket matches while I was working in the Cayman Islands - well, I watched part of them - they're inclined to drag on for days - I never took LSD (was afraid I'd get acid indigestion), but I think if you dropped acid and watched a baseball game, that what you'd see would be cricket. Bob Richmond From c.ingles <@t> hosp.wisc.edu Mon Jul 3 16:23:08 2006 From: c.ingles <@t> hosp.wisc.edu (Ingles Claire) Date: Mon Jul 3 16:24:00 2006 Subject: [Histonet] Tissue-Tek Express References: Message-ID: <583D3E9A1E843445BD54E461D1A2F6F317A04F64@UWHIS-XCHNG2.uwhis.hosp.wisc.edu> OK, Who's channeling Lee Greenwood!?! Claire Ingles ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Lim, Langston (NIH/NCI) [E] Sent: Fri 6/30/2006 12:03 PM To: Timothy Macatee; Bartlett, Jeanine H. (CDC); Goodwin, Diana; Dennis Hahn; HistoNet@Pathology.swmed.edu; kemlo.rogerson@waht.swest.nhs.uk Subject: RE: [Histonet] Tissue-Tek Express To my fellow Americans and veterans: If tomorrow all the things were gone I'd worked for all my life, And I had to start again with just my children and my wife. I'd thank my lucky stars to be living here today, 'Cause the flag still stands for freedom and they can't take that away. And I'm proud to be an American where at least I know I'm free. And I won't forget the men who died, who gave that right to me. And I'd gladly stand up next to you and defend her still today. 'Cause there ain't no doubt I love this land God bless the U.S.A. >From the lakes of Minnesota, to the hills of Tennessee, across the plains of Texas, from sea to shining sea, >From Detroit down to Houston and New York to LA, Well, there's pride in every American heart, and it's time to stand and say: I'm proud to be an American where at least I know I'm free. And I won't forget the men who died, who gave that right to me. And I'd gladly stand up next to you and defend her still today. 'Cause there ain't no doubt I love this land God bless the U.S.A. Langston Lim, HT (ASCP) Tissue Array Research Program DHHS/NIH/NCI/CCR/LP -----Original Message----- From: Timothy Macatee [mailto:timothy.macatee@med.nyu.edu] Sent: Friday, June 30, 2006 12:49 PM To: Bartlett, Jeanine H. (CDC); Goodwin, Diana; Dennis Hahn; HistoNet@Pathology.swmed.edu; kemlo.rogerson@waht.swest.nhs.uk Subject: Re: [Histonet] Tissue-Tek Express Just for anyone who is interested, I found this web site on the American Flag a nice overview. Happy Independent 4th. http://www.ushistory.org/betsy/flagetiq.html#1 Tim On 6/30/06 12:24 PM, "Bartlett, Jeanine (CDC/NCID/VR)" wrote: > Is it okay that I am GOP but don't have a gun? > > > Jeanine Bartlett, BS, HT(ASCP) > (404) 639-3590 > jeanine.bartlett@cdc.hhs.gov > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Goodwin, > Diana > Sent: Friday, June 30, 2006 12:08 PM > To: Dennis Hahn; HistoNet@Pathology.swmed.edu; > kemlo.rogerson@waht.swest.nhs.uk > Subject: RE: [Histonet] Tissue-Tek Express > > Here, Here, and a resounding "Huzzah!" So nice that we bleeding-heart > liberals can wholeheartedly agree and identify with our gun-toting GOP > brethren now and again. > > Now I'm going home to hoist an ice-cold Coors and rent "The Patriot". > > Best Regards to ALL, > Diana Goodwin > > ________________________________ > > From: Dennis Hahn [mailto:DennisH@cookchildrens.org] > Sent: Friday, June 30, 2006 11:56 AM > To: Goodwin, Diana; HistoNet@Pathology.swmed.edu; > kemlo.rogerson@waht.swest.nhs.uk > Subject: RE: [Histonet] Tissue-Tek Express > > > Histo Net, > > Climbs upon his American, gun-toting soap-box.... > > Let the Friday flaming begin. This is not meant to offend any one > nationality. I usually just pick up tid-bits of information from the > histonet, and ignore all the usual comments or flamings. > > However, maybe the wording was not meant to be what I interpret it as, > but... > > 1) We were not "made" independent from anyone. No other country, or > king, gave us our independence. We fought for it. I served in our armed > forces for 6 years active duty, including the "first" gulf "conflict" as > it is referred to. I fly an American flag in my front yard and do my > best to explain to my children each time we take it down and burn it > (Yes, you SHOULD burn the American flag when it is worn out, and no > longer usable...it represents our country, a living, breathing entity) > that many men, and women, have died so that the people in our nation, > and others, may vote for our representatives both locally and > nationally, say what we want, when we want, come and go throughout our > country and the world as we please, all without the fear of any > retaliation when we lay our heads down at night to sleep because of it. > > 2) I'm not saying that anyone should be sad, but in less than 230 years, > the US did GAIN it's independence, survive a brief civil war, establish > a free, and hopefully, equal society, spearhead many of the industrial > revolution ideas (That cotton gin thing really had the world stumped, > didn't it?) and create the most sophisticated military the planet has > ever known. Yes, we do have our problems...The elderly, poor, and many > children do not have access to adequate health care. > > 3) I do believe that Diana Goodwin stated, and I quote, "Happy > Independence Day to all my fellow Americans!" Why respond at all if it > doesn't apply to you? > > 4) Let all of my fellow Americans remember ALL the soldiers this weekend > that are currently scattered throughout the world. Many are in places > and battles that we will never know about. > > Climbs down off his soapbox, gets some BBQ to eat, and is ready for > fireworks. > > Dennis Hahn > dennish@cookchildrens.org > > >>>> Kemlo Rogerson 6/30/2006 8:52 AM >>>> > > Question? Can we say 'Happy Independence Day'? It was from us you were > made > independent from, so should we be sad? > > Kemlo Rogerson > Pathology Manager > Ext 3311 > DD 01934 647057 > Mob 07749 754194 > Pager 07659 597107 > > Distance is meant to relate, not separate. --Satish Kumar > > > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on > its > contents: to do so is strictly prohibited and may be unlawful. Please > inform > me that this message has gone astray before deleting it. Thank you for > your > co-operation > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------------------------------------------------ > ------------------------------------ > Cook Children's Health Care System > > This e-mail, facsimile, or letter and any files or attachments > transmitted may contain information that is confidential and privileged. > This information is intended only for the use of the individual(s) and > entity(ies) to whom it is addressed. If you are the intended recipient, > further disclosures are prohibited without proper authorization. If you > are not the intended recipient, any disclosure, copying, printing, or > use of this information is strictly prohibited and possibly a violation > of federal or state law and regulations. > > If you have received this information in error, please notify Cook > Children's Health Care System immediately at (682)885-4000 or via e-mail > at compliance@cookchildrens.org. Cook Children's Health Care System, its > subsidiaries, and affiliates hereby claim all applicable privileges > related to this information. > ------------------------------------------------------------------------ > ----------------------------------- > > > The information contained in this e-mail message is intended only for > the personal and confidential use of the recipient(s) named above. If > the reader of this message is not the intended recipient or an agent > responsible for delivering it to the intended recipient, you are hereby > notified that you have received this document in error and that any > review, dissemination, distribution, or copying of this message is > strictly prohibited. If you have received this communication in error, > please notify us immediately by e-mail, and delete the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Tim Macatee Research Histology Core New York University School of Medicine 550 First Ave. Department of Pathology. Medical Science Building - Room 521 New York, N.Y. 10016 (212) 263-3888 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From antje.marcantonio <@t> novartis.com Tue Jul 4 02:10:54 2006 From: antje.marcantonio <@t> novartis.com (antje.marcantonio@novartis.com) Date: Tue Jul 4 02:11:15 2006 Subject: [Histonet] veterinary B cell IHC marker Message-ID: Hello Jan are you talking about the CD20cy, clone L26 from Dako? We use this one successfully as B cell marker in our non-human primate tissue, FFPE I cannot believe they discontinue this ab since they introduced a new RTU configuration in April this year ?! Regards, Antje Antje Marcantonio Novartis Pharma AG PH214217, AT TX: LAB. WIECZOREK CHBS, WSJ-386.5.55 Novartis Pharma AG Lichtstrasse 35 CH-4056 Basel Switzerland Phone: +41 61 3246730 Fax: +41 61 3247534 Email : antje.marcantonio@novartis.com CONFIDENTIALITY NOTICE The information contained in this e-mail message is intended only for the exclusive use of the individual or entity named above and may contain information that is privileged, confidential or exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, or the employee or agent responsible for delivery of the message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by e-mail and delete the material from any computer.. Thank you From PKamalavenkatesh <@t> wockhardtin.com Tue Jul 4 03:24:09 2006 From: PKamalavenkatesh <@t> wockhardtin.com (PKamalavenkatesh@wockhardtin.com) Date: Tue Jul 4 03:17:31 2006 Subject: [Histonet] Detection of phospholipidosis -reg Message-ID: Dear Histonetters, This is with regard to detection of phospholipidosis in rodent tissue sections. I was told to develop a rapid, sensitive screening method for detection of phospholipidosis in rodent tissues. The chemicals I am dealing with are known for inducing phospholipidosis in liver, spleen, kidney, adrenals and eye. In the literature, it was recommended that ultra structural examination of the sections is the gold standard for detection of phospholipidosis (presence of lamellar bodies in lysosomes). Histologically, the only changes observed are the vacuolations in the respective tissues. Also acid phosphatase and nile red staining are recommended as marker of lysosomal storage disorders and histochemical staining of phospholipids respectively. Now my question is whether any of our members are having any sort of experience to deal with this since doing Electron Microscopy for regular screening will be very difficult. Hence, my request is anybody can give me a sound advice to recommend a procedure for rapid and sensitive detection of phospholipidosis in rodents. Thanks and regards REGARDS Dr.P.Kamalavenkatesh New Drug Discovery- Biology Wockhardt Research Center D-4, MIDC, Chikalthana Aurangabad. Maharastra, India E.mail : Pkamalavenkatesh@wockhardtin.com Please Visit our New Corporate Web Site www.wockhardt.com --------------------- Disclaimer ------------------------------------------------------------------------------ Information transmitted by this E-MAIL is proprietary to Wockhardt Ltd. and/ or its Group Companies and/or,its Customers and is intended for use only by the individual or entity to which it is addressed, and may contain information that is privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. --------------------------------------------------------------------------------------------------------------- From kemlo.rogerson <@t> waht.swest.nhs.uk Tue Jul 4 05:05:43 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Jul 4 05:05:03 2006 Subject: [Histonet] Re: FRIDAY musing Message-ID: But you miss the point; cricket is played by gentlemen or those we taught. It is a fine game based on good communication, manners, leadership and rules we Englishmen are taught as babies; it's where good chaps get rid of their aggression in a controlled civilised way. Then there is baseball....... Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 We are all broken and wounded in this world. Some choose to grow strong at the broken places. --Harold J. Duarte-Bernhardt This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From jnocito <@t> satx.rr.com Tue Jul 4 08:51:07 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Jul 4 08:51:00 2006 Subject: [Histonet] FRIDAY Musing References: Message-ID: <001e01c69f70$e6b171a0$0b69ce44@yourxhtr8hvc4p> cricket? We use those as bait to go fishing Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Rittman, Barry R" To: "Chris Pomajzl" ; "HISTONET" Sent: Monday, July 03, 2006 8:38 AM Subject: RE: [Histonet] FRIDAY Musing Speaking as someone born in Britain but who has lived in America for 38 years...Baseball is much more exciting than cricket. Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Chris Pomajzl Sent: Mon 7/3/2006 8:35 AM To: HISTONET Subject: Re: [Histonet] FRIDAY Musing Then we can assume that all of your ladies are 6'4" and weigh 240 lbs. (109kg for the Brits) ; ) Ha! Baseball = Uncivilized Cricket ----- Original Message ----- From: "Kemlo Rogerson" To: "'Breeden, Sara'" ; Sent: Monday, July 03, 2006 2:02 AM Subject: RE: [Histonet] FRIDAY Musing > What is base ball? I know Rugby, I know Football (Soccer), I know American > Football (Rugby with armour on) and I even know Australian Football > (American Football without the armour) and even that thing they do it > Ireland but I don't know its name; is Base Ball like 'Rounders'? If so > that's a spiffing game, old chap, and I'd love to see a game; mostly the > ladies play it here. > > Kemlo Rogerson > Pathology Manager > Ext 3311 > DD 01934 647057 > Mob 07749 754194 > Pager 07659 597107 > > Distance is meant to relate, not separate. --Satish Kumar > > > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on its > contents: to do so is strictly prohibited and may be unlawful. Please inform > me that this message has gone astray before deleting it. Thank you for your > co-operation > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.394 / Virus Database: 268.9.8/380 - Release Date: 6/30/2006 From timo.vaisanen <@t> oulu.fi Tue Jul 4 10:22:52 2006 From: timo.vaisanen <@t> oulu.fi (Timo =?iso-8859-1?b?VuRpc+RuZW4=?=) Date: Tue Jul 4 10:23:00 2006 Subject: [Histonet] Concave paraffin blocks Message-ID: <1152026572.44aa87ccd8252@webmail.oulu.fi> Dear all, I mailed a while ago a question about concave paraffin blocks and received ample of suggestions. Thank you all. Based on them the culprit for our problem was suggested to be the excess of xylene in the blocks. I have now checked the tissue processors for faulty valves causing carry-over etc. and they seemed to be fine. Concave blocks were also present when processing was performed with totally fresh paraffin. Therefore, I think, carry-over of solvents may not be the cause in our case. Somebody also suggested that too low temperature of the cold plate during embedding or when cooling the blocks before sectioning could cause the concave surface. I tested this by adjusting the plate temperatures to -5-10C range. This did not help. The only thing that seemed to have some kind of positive effect was using six chamber biopsy cassettes during the embedding. The rationale in this experiment was, that maybe during embedding and when paraffin solidifies the shape of the cassette base changes a littel bit and causes tension to the solid paraffin. This tension could then cause the concave shape of the block surface. The extra plastic in the six chamber biopsy cassette base could make it more rigid and resistant to tension/distortion. To my surprise using these cassette bases made the blocks more even. Does this theory make any sense? One other thing that came to my mind is paraffin itself. We routinely use Histowax 52-54C. Maybe I should try another brand? Thank you for your comments in advance! Timo Univ. Hospital of Oulu Dept. of Pathology Finland From gu.lang <@t> gmx.at Tue Jul 4 10:50:48 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Jul 4 10:51:01 2006 Subject: [Histonet] nomenclature oncogenes Message-ID: <000001c69f81$a05d6270$eeeea8c0@SERVER01> Hi histonetters, I am looking for a website with a list of the main genes/proteins of cellcycle and tumorigenesis (not the whole database), where I can see the abbreviations and full names. Perhaps someone can help me? Gudrun Lang From sbreeden <@t> nmda.nmsu.edu Tue Jul 4 14:10:57 2006 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Jul 4 14:11:02 2006 Subject: [Histonet] BASEBALL & OTHER ROUND SPORTS Message-ID: I KNEW I should not have taken this long weekend since I posted that come-on for a baseball game during NSH! Now, I've got to address all those folks who think baseball is descended from some OTHER sport! Obviously, none of you are coming to PHX for NSH so I'll leave you to watch your soccer/cricket/football games in ignorance (do I have to plug in my All-Purpose Disclaimer here??). Baseball is an American sport and should be watched from seats close to the baseline or directly behind home plate while eating a hot dog and drinking a slightly yellow, effervescent, foul-tasting (oh, boy, I'm gonna catch it now!) liquid with a foamy surface. So, I restate my question: Is anyone interested in getting a group together to see a BASEBALL game between the Arizona Diamondbacks and the St. Louis Cardinals or Washington during NSH in Phoenix, Arizona (that's in the United States, by the way...and New Mexico is BETWEEN Arizona and Texas, in case your geography's a bit rusty). I have had some interested parties respond... I'm done loading the processor for tomorrow now, so I'm going on to the house (that's New Mexican for "goin' home"). Hasta la poop. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87108 From histosci <@t> shentel.net Tue Jul 4 16:28:27 2006 From: histosci <@t> shentel.net (HSRL) Date: Tue Jul 4 16:28:43 2006 Subject: [Histonet] Special stain kits Message-ID: <000001c69fb0$cd847bb0$0500a8c0@HSRLMAIN> Dear Netters, We are looking for a new supplier for our special stain kits. Common specials include: Trichrome, GMS, PAS, Congo Red, LFB/CV. Could someone recommend a good supplier? Thanks in advance, Beth Beth Poole HSRL, Inc.- A GLP Compliant Contract Laboratory 5930 Main Street Mount Jackson, Virginia 22842 540.477.4440 Fax: 540.477.4448 beth@hsrl.org www.hsrl.org From histosci <@t> shentel.net Tue Jul 4 16:46:05 2006 From: histosci <@t> shentel.net (HSRL) Date: Tue Jul 4 16:46:19 2006 Subject: [Histonet] Microwave for Decalcification Message-ID: <000001c69fb3$43f2dd30$0500a8c0@HSRLMAIN> Netters, Another question for you: Is anyone using a microwave to decalcify bones or to expedite special staining? I know the processing microwaves have this capability, but we would rather use a laboratory grade microwave for these procedures. Again, your help is greatly appreciated. -Beth Beth Poole HSRL, Inc.- A GLP Compliant Contract Laboratory 5930 Main Street Mount Jackson, Virginia 22842 540.477.4440 Fax: 540.477.4448 beth@hsrl.org www.hsrl.org From mtyler <@t> uctgsh1.uct.ac.za Wed Jul 5 01:06:03 2006 From: mtyler <@t> uctgsh1.uct.ac.za (Tyler) Date: Wed Jul 5 01:06:12 2006 Subject: [Histonet] neutrophil Message-ID: <44AB56CB.EE91E630@uctgsh1.uct.ac.za> Morning All Please you can help. Need a method for neutrophil peroxidase activity in mouse lung after LPS. This is all the information I have been given.Thanks in advance. Marilyn From cpomajzl <@t> cpllabs.com Wed Jul 5 06:30:50 2006 From: cpomajzl <@t> cpllabs.com (Chris Pomajzl) Date: Wed Jul 5 06:26:00 2006 Subject: [Histonet] BASEBALL & OTHER ROUND SPORTS References: Message-ID: <00a501c6a026$7850a3f0$26fca8c0@CSP> Why would anyone want to drink urine at a baseball game??? ; ) ----- Original Message ----- From: "Breeden, Sara" To: Sent: Tuesday, July 04, 2006 2:10 PM Subject: [Histonet] BASEBALL & OTHER ROUND SPORTS I KNEW I should not have taken this long weekend since I posted that come-on for a baseball game during NSH! Now, I've got to address all those folks who think baseball is descended from some OTHER sport! Obviously, none of you are coming to PHX for NSH so I'll leave you to watch your soccer/cricket/football games in ignorance (do I have to plug in my All-Purpose Disclaimer here??). Baseball is an American sport and should be watched from seats close to the baseline or directly behind home plate while eating a hot dog and drinking a slightly yellow, effervescent, foul-tasting (oh, boy, I'm gonna catch it now!) liquid with a foamy surface. So, I restate my question: Is anyone interested in getting a group together to see a BASEBALL game between the Arizona Diamondbacks and the St. Louis Cardinals or Washington during NSH in Phoenix, Arizona (that's in the United States, by the way...and New Mexico is BETWEEN Arizona and Texas, in case your geography's a bit rusty). I have had some interested parties respond... I'm done loading the processor for tomorrow now, so I'm going on to the house (that's New Mexican for "goin' home"). Hasta la poop. Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87108 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo.rogerson <@t> waht.swest.nhs.uk Wed Jul 5 06:57:32 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Jul 5 06:56:55 2006 Subject: [Histonet] BASEBALL & OTHER ROUND SPORTS Message-ID: I thought that, why would be people drink urine AND watch baseball, when you could just drink urine? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 We are all broken and wounded in this world. Some choose to grow strong at the broken places. --Harold J. Duarte-Bernhardt This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From cgfields <@t> lexhealth.org Wed Jul 5 09:32:41 2006 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Wed Jul 5 09:28:23 2006 Subject: [Histonet] Special stain kits Message-ID: We get most everything from Poly Scientific. Rowley is a good source and American Mastertech also. Carole Fields, HT,ASCP Pathology Supervisor Lexington Medical Center 2720 Sunset Blvd. W. Columbia, SC 29169 -----Original Message----- From: HSRL [mailto:histosci@shentel.net] Sent: Tuesday, July 04, 2006 5:28 PM To: histonet@pathology.swmed.edu Subject: [Histonet] Special stain kits Dear Netters, We are looking for a new supplier for our special stain kits. Common specials include: Trichrome, GMS, PAS, Congo Red, LFB/CV. Could someone recommend a good supplier? Thanks in advance, Beth Beth Poole HSRL, Inc.- A GLP Compliant Contract Laboratory 5930 Main Street Mount Jackson, Virginia 22842 540.477.4440 Fax: 540.477.4448 beth@hsrl.org www.hsrl.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. From pmarcum <@t> vet.upenn.edu Wed Jul 5 10:09:48 2006 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Wed Jul 5 10:10:00 2006 Subject: [Histonet] Special stain kits In-Reply-To: References: Message-ID: <6.1.1.1.2.20060705110905.01a2ff38@mail.vet.upenn.edu> Special stains we don't make on site buy from Poly Scientific and they have worked very well for us. Pam Marcum At 10:32 AM 7/5/2006, Carole Fields wrote: >We get most everything from Poly Scientific. Rowley is a good source and >American Mastertech also. > >Carole Fields, HT,ASCP >Pathology Supervisor >Lexington Medical Center >2720 Sunset Blvd. >W. Columbia, SC 29169 > > > > >-----Original Message----- >From: HSRL [mailto:histosci@shentel.net] >Sent: Tuesday, July 04, 2006 5:28 PM >To: histonet@pathology.swmed.edu >Subject: [Histonet] Special stain kits > > >Dear Netters, > > > >We are looking for a new supplier for our special stain kits. Common >specials include: Trichrome, GMS, PAS, Congo Red, LFB/CV. Could >someone recommend a good supplier? > > > >Thanks in advance, > > > >Beth > > > >Beth Poole > >HSRL, Inc.- A GLP Compliant Contract Laboratory > >5930 Main Street > >Mount Jackson, Virginia 22842 > >540.477.4440 > >Fax: 540.477.4448 > >beth@hsrl.org > >www.hsrl.org > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_________________________________ >This email and any files transmitted with it may contain PRIVILEGED or >CONFIDENTIAL information and may be read or used only by the intended >recipient. If you are not the intended recipient of the email or any of its >attachments, please be advised that you have received this email in error >and that any use, dissemination, distribution, forwarding, printing, or >copying of this email or any attached files is strictly prohibited. If you >have received this email in error, please immediately purge it and all >attachments and notify the sender by reply email or contact the sender at >the number listed above if one is provided. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From jnocito <@t> pathreflab.com Wed Jul 5 11:46:01 2006 From: jnocito <@t> pathreflab.com (Joe Nocito) Date: Wed Jul 5 11:42:49 2006 Subject: [Histonet] Re: FRIDAY musing In-Reply-To: Message-ID: Well, that's the first mistake. Who here is a gentleman? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kemlo Rogerson Sent: Tuesday, July 04, 2006 5:06 AM To: 'RSRICHMOND@aol.com'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Re: FRIDAY musing But you miss the point; cricket is played by gentlemen or those we taught. It is a fine game based on good communication, manners, leadership and rules we Englishmen are taught as babies; it's where good chaps get rid of their aggression in a controlled civilised way. Then there is baseball....... Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 We are all broken and wounded in this world. Some choose to grow strong at the broken places. --Harold J. Duarte-Bernhardt This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mtitford <@t> aol.com Wed Jul 5 12:12:51 2006 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Wed Jul 5 12:13:01 2006 Subject: [Histonet] Pathology teaching question Message-ID: <8C86E6D61CF3450-474-539@mblk-d51.sysops.aol.com> First of all, I hope all the American Histonetters enjoyed their fouth of July. ( I hope all the OTHER histonetters enjoyed their fourth of July too, but they probably did not get barbecued ribs, cake and ice cream, a fireworks display and all that other stuff !) Question: We have a collection of "wet" pathology specimens we use to teach sophomore medical students gross pathology. How do other medical schools label such specimens? We have been the plastination route, and the potting in plexiglass (Perspex) route, and have found the medical students learn best when they can actually handle the specimens. Anyone have any ideas on labeling? Thank you Mike Titford USA Pathology Mobile AL USA ________________________________________________________________________ Check out AOL.com today. Breaking news, video search, pictures, email and IM. All on demand. Always Free. From SHARON.OSBORN <@t> SPCORP.COM Wed Jul 5 12:20:36 2006 From: SHARON.OSBORN <@t> SPCORP.COM (Osborn, Sharon) Date: Wed Jul 5 12:20:50 2006 Subject: [Histonet] Baseball Message-ID: <9A919A5D70313A4D9C56A025710874080C732B@kenmsg40.us.schp.com> Guys/Gals! Baseball is evolved from the American Indian game of "stickball". It is truly an American game w/o origins from other sports. sharon osborn DNAX Palo Alto, CA ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From Heidi.Miers <@t> NAU.EDU Wed Jul 5 12:26:20 2006 From: Heidi.Miers <@t> NAU.EDU (Heidi Miers) Date: Wed Jul 5 12:26:24 2006 Subject: [Histonet] BASEBALL & OTHER ROUND SPORTS Message-ID: <44ABF63C.8040308@nau.edu> I don't know if I am going yet but I would love to watch against the Cards since I am keen on them. I will send notice if I am going. Heidi Heidi Miers Research Specialist, Sr. Imaging and Histology Core Facility Discovery Research Laboratories Northern Arizona University Heidi.Miers@nau.edu 928-523-6725 From sbreeden <@t> nmda.nmsu.edu Wed Jul 5 13:03:43 2006 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Jul 5 13:03:49 2006 Subject: [Histonet] RE: Histonet Digest, Vol 32, Issue 5 In-Reply-To: <20060705170606.0208FB4095@ccserver4.nmsu.edu> Message-ID: I may not stir up much brouhaha about technical Stuff, but just mention "baseball" and all **** breaks loose. Sigh.... Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87108 From themagoos <@t> rushmore.com Wed Jul 5 14:31:22 2006 From: themagoos <@t> rushmore.com (themagoos@rushmore.com) Date: Wed Jul 5 14:49:57 2006 Subject: [Histonet] Fungus GMS Control Message-ID: <1152127882.44ac138ad0ac4@webmail.rushmore.com> My lab is in desperate need for fungus (GMS) control blocks. We have several AFB control blocks and are willing to trade for GMS control blocks. Please contact me if you can help. Jason McGough HT(ASCP) Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 jmcgough@clinlab.com ------------------------------------------------- This mail sent by http://webmail.rushmore.com From omnivore98 <@t> yahoo.com Wed Jul 5 15:19:48 2006 From: omnivore98 <@t> yahoo.com (Heather Renko) Date: Wed Jul 5 15:19:54 2006 Subject: [Histonet] embedding and cutting speeds Message-ID: <20060705201948.63110.qmail@web31303.mail.mud.yahoo.com> Hospital based anatomic path Histonetters, Can I get some opinions on what the average blocks per hour are for experienced to beginner embedding and cutting on manual and electric microtomes. I am only concerned with just an average and I know that its quality not quantity. Being hospital based we are dealing with many tissue types. Thank you in advance. --------------------------------- Sneak preview the all-new Yahoo.com. It's not radically different. Just radically better. From Rae.Staskiewicz <@t> Illinois.gov Wed Jul 5 15:37:17 2006 From: Rae.Staskiewicz <@t> Illinois.gov (Staskiewicz, Rae) Date: Wed Jul 5 15:39:07 2006 Subject: [Histonet] Digital Imaging systems Message-ID: <0909DF46D9192E45A16DC6254AA853D41CE34B@IL084EX102.Illinois.gov> We are looking for information on digital imaging systems. Especially Nikon Optiphot-2. Any vendors are welcome to contact us. Thanks in advance for your help. Rae Ann Staskiewicz Section Head-Histology Galesburg Animal Disease Lab Galesburg, IL 309-344-2451 From JGREWE <@t> OhioHealth.com Wed Jul 5 15:57:11 2006 From: JGREWE <@t> OhioHealth.com (JGREWE@OhioHealth.com) Date: Wed Jul 5 15:57:22 2006 Subject: [Histonet] Jacquelyn Grewe/Staff/OhioHealth is out of the office . Message-ID: I will be out of the office starting 07/05/2006 and will not return until 07/17/2006. I will respond to your message when I return. Thanks, Jackie From jnocito <@t> satx.rr.com Wed Jul 5 17:58:48 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Jul 5 17:58:56 2006 Subject: [Histonet] Concave paraffin blocks References: <1152026572.44aa87ccd8252@webmail.oulu.fi> Message-ID: <003301c6a086$952acdb0$0b69ce44@yourxhtr8hvc4p> Timo, I understand the reasoning behind using the 6 chamber blocks. It's like when contractors place steel bars when they pour concrete, it reinforces the concrete. However, I don't think this would have any effect on the cutting surface of the block. I'm not familiar with Histowax so I can not intelligently say it is the embedding medium or not. How long has this problem been happening? Have you changed anything in the processing of your blocks before this problem started? I'm sorry I did not see your first posting. Have you tried a different paraffin? Can you get some samples from your supplier? If you can't, please email me. I'll see what I can do. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Timo V?is?nen" To: Sent: Tuesday, July 04, 2006 10:22 AM Subject: [Histonet] Concave paraffin blocks > Dear all, > > I mailed a while ago a question about concave paraffin blocks and received > ample > of suggestions. Thank you all. Based on them the culprit for our problem > was > suggested to be the excess of xylene in the blocks. > I have now checked the tissue processors for faulty valves causing > carry-over > etc. and they seemed to be fine. Concave blocks were also present when > processing was performed with totally fresh paraffin. Therefore, I think, > carry-over of solvents may not be the cause in our case. Somebody also > suggested that too low temperature of the cold plate during embedding or > when > cooling the blocks before sectioning could cause the concave surface. I > tested > this by adjusting the plate temperatures to -5-10C range. This did not > help. > The only thing that seemed to have some kind of positive effect was using > six > chamber biopsy cassettes during the embedding. The rationale in this > experiment > was, that maybe during embedding and when paraffin solidifies the shape of > the > cassette base changes a littel bit and causes tension to the solid > paraffin. > This tension could then cause the concave shape of the block surface. The > extra > plastic in the six chamber biopsy cassette base could make it more rigid > and > resistant to tension/distortion. To my surprise using these cassette bases > made > the blocks more even. Does this theory make any sense? One other thing > that came > to my mind is paraffin itself. We routinely use Histowax 52-54C. Maybe I > should > try another brand? > Thank you for your comments in advance! > > Timo > Univ. Hospital of Oulu > Dept. of Pathology > Finland > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.394 / Virus Database: 268.9.9/382 - Release Date: 7/4/2006 > > From Jacqueline.Malam <@t> rli.mbht.nhs.uk Thu Jul 6 03:26:39 2006 From: Jacqueline.Malam <@t> rli.mbht.nhs.uk (Malam Jacqueline) Date: Thu Jul 6 03:28:50 2006 Subject: [Histonet] Re- microwave special stains Message-ID: We use the microwave for the Grimelius silver method - we made up our own version Jacqui Malam Lancaster UK DISCLAIMER: This e-mail is confidential and privileged. If you are not the intended recipient please accept our apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform postmaster@rli.mbht.nhs.uk that this message has gone astray before deleting it. Comments or opinions expressed in this email are those of their respective contributors only. The views expressed do not represent the views of the Trust, its management or employees. University Hospitals of Morecambe Bay NHS Trust is not responsible and disclaims any and all liability for the content of comments written within.Thank you for your co-operation. From BMolinari <@t> heart.thi.tmc.edu Thu Jul 6 06:14:13 2006 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Thu Jul 6 06:14:16 2006 Subject: [Histonet] Baseball/stickball Message-ID: Sharon, Growing up in the city, we did not have any space to play baseball so stickball was the norm. We used a small rubber ball, about the size of a tennis ball, that was cut in half. Not only a skill to hit but to learn to throw as well. Thanks for bringing back the memory! Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Osborn, Sharon Sent: Wednesday, July 05, 2006 12:21 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Baseball Guys/Gals! Baseball is evolved from the American Indian game of "stickball". It is truly an American game w/o origins from other sports. sharon osborn DNAX Palo Alto, CA ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From galinadeyneko <@t> yahoo.com Thu Jul 6 09:44:58 2006 From: galinadeyneko <@t> yahoo.com (Galina Deyneko) Date: Thu Jul 6 09:45:02 2006 Subject: [Histonet] (no subject) Message-ID: <20060706144458.2176.qmail@web33105.mail.mud.yahoo.com> Hi Beth I would like to recommend Poly Scientific R&D Corp.,phone 800-645-5825, 800-832-5869. The others: Rowley Biochemical Inc. 978-739-4883, or Master Tech 800-860-4073. Galina Deyneko Cambridge, MA, Novartis --------------------------------- How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. From galinadeyneko <@t> yahoo.com Thu Jul 6 09:58:16 2006 From: galinadeyneko <@t> yahoo.com (Galina Deyneko) Date: Thu Jul 6 09:58:22 2006 Subject: [Histonet] monkey'heart Message-ID: <20060706145816.86904.qmail@web33101.mail.mud.yahoo.com> Dear Colleagues. Can you share with me the protocol and you expertise in processing for paraffin embedding of monkey' hearts. Thank you in advance. Galina Deyneko Novartis Cambridge, MA --------------------------------- Do you Yahoo!? Next-gen email? Have it all with the all-new Yahoo! Mail Beta. From crains <@t> wpmpath.com Thu Jul 6 11:37:37 2006 From: crains <@t> wpmpath.com (crains@wpmpath.com) Date: Thu Jul 6 11:37:47 2006 Subject: [Histonet] Disposable blades Message-ID: <20060706163737.BKQH3326.dukecmmtao03.coxmail.com@dukecmmtao03> In the process of changing to disposable microtome blades. Any feedback on which brands work best, or which ones to avoid? Thanks. Chris Rains WPM Pathology laboratory Salina, KS From settembr <@t> umdnj.edu Thu Jul 6 11:41:15 2006 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Thu Jul 6 11:42:12 2006 Subject: [Histonet] Disposable blades Message-ID: Accu-Edge Dana Settembre University Hospital - UMDNJ Newark, NJ >>> 07/06/06 12:37 PM >>> In the process of changing to disposable microtome blades. Any feedback on which brands work best, or which ones to avoid? Thanks. Chris Rains WPM Pathology laboratory Salina, KS _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From livieira <@t> ualg.pt Thu Jul 6 11:55:19 2006 From: livieira <@t> ualg.pt (Lina Vieira) Date: Thu Jul 6 11:52:24 2006 Subject: [Histonet] Re: Disposable blades Message-ID: <001501c6a11c$f704c900$2914100a@labhistologia> FeatherT disposable blades -S35 are good. Lina Vieira Algarve University Portugal From rjbuesa <@t> yahoo.com Thu Jul 6 12:09:18 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jul 6 12:09:23 2006 Subject: [Histonet] Disposable blades In-Reply-To: <20060706163737.BKQH3326.dukecmmtao03.coxmail.com@dukecmmtao03> Message-ID: <20060706170918.72270.qmail@web61223.mail.yahoo.com> Chris: You will receive many answers that will reflect personal preferences. Under that context I can tell you that Accu-Edge (from Feather sold by Sakura) are the best. You could end tabulating the answers to take a decission. Ren? J. crains@wpmpath.com wrote: In the process of changing to disposable microtome blades. Any feedback on which brands work best, or which ones to avoid? Thanks. Chris Rains WPM Pathology laboratory Salina, KS _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Sneak preview the all-new Yahoo.com. It's not radically different. Just radically better. From pmarcum <@t> vet.upenn.edu Thu Jul 6 12:40:36 2006 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Thu Jul 6 12:40:49 2006 Subject: [Histonet] Disposable blades In-Reply-To: <20060706163737.BKQH3326.dukecmmtao03.coxmail.com@dukecmmta o03> References: <20060706163737.BKQH3326.dukecmmtao03.coxmail.com@dukecmmtao03> Message-ID: <6.1.1.1.2.20060706133904.0196bac0@mail.vet.upenn.edu> We use the Shandon MX35 Premier + and they are very good. Good life on the edge and more economical than Accu Edge. Pam Marcum UPenn Vet School At 12:37 PM 7/6/2006, crains@wpmpath.com wrote: >In the process of changing to disposable microtome blades. Any feedback >on which brands work best, or which ones to avoid? Thanks. > >Chris Rains >WPM Pathology laboratory >Salina, KS > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jbaez <@t> interscopepath.com Thu Jul 6 12:52:50 2006 From: jbaez <@t> interscopepath.com (Baez, Janet) Date: Thu Jul 6 12:52:59 2006 Subject: [Histonet] Disposable blades Message-ID: <9E956D8FEB06C2408B08AC16498325E9FF69@adsl-67-113-77-28.dsl.lsan03.pacbell.net> Accu-Edge from Sakura is the best. Janet Baez Interscope Pathology Canoga Park, Ca. -----Original Message----- From: crains@wpmpath.com [mailto:crains@wpmpath.com] Sent: Thursday, July 06, 2006 9:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Disposable blades In the process of changing to disposable microtome blades. Any feedback on which brands work best, or which ones to avoid? Thanks. Chris Rains WPM Pathology laboratory Salina, KS _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RBARNHART <@t> summithealth.org Thu Jul 6 12:57:47 2006 From: RBARNHART <@t> summithealth.org (Rebecca Barnhart) Date: Thu Jul 6 12:58:13 2006 Subject: [Histonet] Lab assistant working in histology Message-ID: Does anyone know of a regulation (CLIA, CAP, JACHO) preventing a lab assistant from working in histology (embedding, cutting, staining, coverslipping and changing solutions)? Thanks! From godsgalnow <@t> aol.com Thu Jul 6 13:06:48 2006 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Thu Jul 6 13:07:00 2006 Subject: [Histonet] Lab assistant working in histology In-Reply-To: References: Message-ID: <8C86F3E154D705B-338-1316@FWM-M27.sysops.aol.com> There is nothing wrong with having a lab assistant changing stainers, processors, or coverslipping, as long as they are properly trained-but tech work is a no no. Roxanne Soto Clinical Lab Manager Physicians RightPath Tampa, Florida -----Original Message----- From: Rebecca Barnhart To: histonet@lists.utsouthwestern.edu Sent: Thu, 6 Jul 2006 13:57:47 -0400 Subject: [Histonet] Lab assistant working in histology Does anyone know of a regulation (CLIA, CAP, JACHO) preventing a lab assistant from working in histology (embedding, cutting, staining, coverslipping and changing solutions)? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Check out AOL.com today. Breaking news, video search, pictures, email and IM. All on demand. Always Free. From RBARNHART <@t> summithealth.org Thu Jul 6 13:11:14 2006 From: RBARNHART <@t> summithealth.org (Rebecca Barnhart) Date: Thu Jul 6 13:11:55 2006 Subject: [Histonet] Lab assistant working in histology Message-ID: I am sorry I forgot to mention I work in Pennsylvania if that makes a difference. >>> 7/6/2006 2:06:48 PM >>> There is nothing wrong with having a lab assistant changing stainers, processors, or coverslipping, as long as they are properly trained-but tech work is a no no. Roxanne Soto Clinical Lab Manager Physicians RightPath Tampa, Florida -----Original Message----- From: Rebecca Barnhart To: histonet@lists.utsouthwestern.edu Sent: Thu, 6 Jul 2006 13:57:47 -0400 Subject: [Histonet] Lab assistant working in histology Does anyone know of a regulation (CLIA, CAP, JACHO) preventing a lab assistant from working in histology (embedding, cutting, staining, coverslipping and changing solutions)? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Check out AOL.com today. Breaking news, video search, pictures, email and IM. All on demand. Always Free. From rjbuesa <@t> yahoo.com Thu Jul 6 13:53:19 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jul 6 13:53:25 2006 Subject: [Histonet] Lab assistant working in histology In-Reply-To: Message-ID: <20060706185320.31139.qmail@web61217.mail.yahoo.com> Rebecca: That depends on the State you live in. I do not know about Pennsylvalia and you should consult with your local lab authorities. In other States (like Florida) there are regulations and licensure requirements. Licensed tasks like ANY related to the tissue sample itself have to be done by licensed personnel (cassetting, embedding, sectioning, special stains). Unlicensed tasks are those than can be completed automatically (tissue processing, routine staining and coverslipping, labelling slides, collatings reports, changing reagents in processors or stainers, etc.) can be completed by unlicensed personnel. The raitonale is that when dealing with tissues and samples there should be a certaining training level and a license to credit it. Hope this will help you. Ren? J. Rebecca Barnhart wrote: Does anyone know of a regulation (CLIA, CAP, JACHO) preventing a lab assistant from working in histology (embedding, cutting, staining, coverslipping and changing solutions)? Thanks! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. From tkngflght <@t> yahoo.com Thu Jul 6 13:59:00 2006 From: tkngflght <@t> yahoo.com (Cheryl) Date: Thu Jul 6 13:59:05 2006 Subject: [Histonet] Lab assistant working in histology Message-ID: <20060706185900.80611.qmail@web50908.mail.yahoo.com> Rebecca-- First of couse, is your lab governed by these agencies? All three? A lab assistant can do any or all of these depending on how the work is distributed. PA is not CLIA mandatory to the best of my understanding (Florida is--so it'd be a no-no in Florida) so it's going to depend on your lab, and your manager should be able to help research the statues and rules. One thing I often have to do is call the governing body and ask! Easiest way is email then you have a paper response. I know a number of labs that have the assistants do a all but the cutting as long as the work is 'checked' by a tech ( while cutting or at block check/signout), but even this might not be required. It might be easier to help you if you'd tell us why you want to do this? Is it to train the assistant to become a tech, or just not enough bodies, or cost savings for the facility? There are ways to create an 'in training' situation that will bridge most of these concerns: the other reasons will have different answers. The thing that might give you the biggest issue is you can't pay one person significantly less for doing the same duties as someone in a higher pay scale. Same as you can't have a volunteer performing tasks that you pay someone else to do in their job. It's called 'internal equity'. Your HR contact should be able to guide you on these things more accurately than I. Hope this helps! Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one Tech at a time. 281.883.7704 c 281.852.9457 o admin@fullstaff.org Sign up for the FREE monthly newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. From christahercher <@t> yahoo.ca Thu Jul 6 14:04:09 2006 From: christahercher <@t> yahoo.ca (christa hercher) Date: Thu Jul 6 14:04:17 2006 Subject: [Histonet] golgi stain Message-ID: <20060706190409.29328.qmail@web56512.mail.re3.yahoo.com> Hello, We are trying to stain human tissue with a variation of the golgi stain. The protocol is: 1) We are using mostly frozen tissue that we fix in formal NO buffer 10%. 2) K2Cr2O7 3% for 2 days (changing solution each day) 3) AgNO3 2% for 2 days - on first day we do a rinse with distilled water then rinse with AgNO3. 4) change to sucrose solution and put in fridge 3-4 days. throughout, the tissue and solution is placed on a rocker we are using a small tissue block but are seeing a lot of blood vessels. my question is if anyone knows a way to avoid staining these blood vessels? Thank you christa masters student mcgill university --------------------------------- All new Yahoo! Mail - --------------------------------- Get a sneak peak at messages with a handy reading pane. From crains <@t> wpmpath.com Thu Jul 6 14:04:05 2006 From: crains <@t> wpmpath.com (crains@wpmpath.com) Date: Thu Jul 6 14:04:18 2006 Subject: [Histonet] (no subject) Message-ID: <20060706190406.HCQC3326.dukecmmtao03.coxmail.com@dukecmmtao03> Thank you all. Seems that Accu Edge wins by a landslide! We have techs who are resistant to using disposables ("that's not how we've always done it") but I think they will come to love it once they get used to it. Our Pathologists are pretty progressive on most everything, so it kind of amazes me that the techs have been able to hold them off on disposables for so long. From langxingpan <@t> pantomics.com Thu Jul 6 14:44:44 2006 From: langxingpan <@t> pantomics.com (Langxing Pan) Date: Thu Jul 6 14:44:53 2006 Subject: [Histonet] RE: information on digital imaging systems, Vol 32, Issue 6, Message: 7 Message-ID: <000601c6a134$a29c2030$210110ac@Familyroom> Dear Ms Straskiewicz, There are many good imaging systems in the market. I have tried several of them. The RT2 camera made by Diagnostic Instruments (http://www.diaginc.com/) seems to be the best, especially for fluorescence imaging. You can contact the manufacturer for your local vendor to arrange a demonstration. We also provide an economic microscope imaging system which includes a Nikon Coolpix 8400 (8M resolution), microscope adapter with high quality relay lens, hand-held remote control and computer image capturing and basic processing software. Depending on the microscope, the system costs about $1,500 to $2,000. If you are interested in the system, please email me and I can send you some image samples and more information about the system. Regards, Langxing Pan, M.D., Ph.D. Pantomics, Inc. 457 Mariposa Street San Francisco, CA 94107, USA Tel: 1-415-863-2380 Fax: 1-510-653-1227 langxingpan@pantomics.com www.pantomics.com Message: 7 Date: Wed, 5 Jul 2006 15:37:17 -0500 From: "Staskiewicz, Rae" Subject: [Histonet] Digital Imaging systems To: "Histonet" Message-ID: <0909DF46D9192E45A16DC6254AA853D41CE34B@IL084EX102.Illinois.gov> Content-Type: text/plain; charset="us-ascii" We are looking for information on digital imaging systems. Especially Nikon Optiphot-2. Any vendors are welcome to contact us. Thanks in advance for your help. Rae Ann Staskiewicz Section Head-Histology Galesburg Animal Disease Lab Galesburg, IL 309-344-2451 From kimtournear <@t> yahoo.com Thu Jul 6 14:45:59 2006 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Thu Jul 6 14:46:03 2006 Subject: [Histonet] Giemsa Stain Message-ID: <20060706194559.57683.qmail@web37715.mail.mud.yahoo.com> Hi there, I'm in need of a recipe for a giemsa stain using the powder. I also need a recipe for making light green from powder. Any help would be appreciated. Kim Tournear, HT (ASCP), QIHC (ASCP) Specialists in Dermatology Tucson, AZ --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail Beta. From galinadeyneko <@t> yahoo.com Thu Jul 6 14:47:59 2006 From: galinadeyneko <@t> yahoo.com (Galina Deyneko) Date: Thu Jul 6 14:48:03 2006 Subject: [Histonet] (no subject) Message-ID: <20060706194759.11537.qmail@web33115.mail.mud.yahoo.com> Einar, We use rat anti-mouse Mac 3 Pharmigen # 550292. Hier in steamer with Dako Target retrieval Solution for 30 min. Incubation 2 Hours at RT or overnight at 4 C. It works wonderful in FFPE mouse aortic root. If you need more detailed protocol, e-mail me. Galina Deyneko. Novartis Cambridge MA --------------------------------- How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. From bjdewe <@t> aol.com Thu Jul 6 16:04:37 2006 From: bjdewe <@t> aol.com (bjdewe@aol.com) Date: Thu Jul 6 16:04:57 2006 Subject: [Histonet] IHC workshop in California Message-ID: <8C86F56ECC96742-1760-264C@MBLK-R02.sysops.aol.com> Hi All, We have had a couple of cancellations so if anyone is interested in coming to our free handson wet workshop, please sign up at the link indicated: http://www.vetmed.ucdavis.edu/vsr/dil/events.html Cheers, Lorie Live as if you were to die tomorrow. Learn as if you were to live forever. - Gandhi - ******************************************************************* Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s)and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ******************************************************************* ________________________________________________________________________ Check out AOL.com today. Breaking news, video search, pictures, email and IM. All on demand. Always Free. From JMacDonald <@t> mtsac.edu Thu Jul 6 16:43:18 2006 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Jul 6 16:43:34 2006 Subject: [Histonet] IHC Workload Message-ID: I have a few questions for IHC labs related to workload and staffing. Thank you. 1. How many slides per day? 2. Are slides processed, embedded, and cut by the IHC staff or elsewhere? 3. Automation or manual. If automation what instrument? 4. Number of staff members to perform the workload? How many histotechs? How many lab assistants? Thanks to all who help with this. Jennifer MacDonald Mt. San Antonio College From kemlo.rogerson <@t> waht.swest.nhs.uk Fri Jul 7 02:06:46 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Jul 7 02:06:13 2006 Subject: [Histonet] Giemsa Stain Message-ID: With respect you have no books over there? Giemsa; methylene blue eosinate 4g, azure B eosinate 5g, azure A eosinate 1g and methylene blue chloride (85 to 88% dye content) 2g; keep as dry stain in a cool dry place tightly stoppered. The solvent is an equal volume of 100% ethyl alcohol and 98% glycerol and the dye is dissolved 800mg/ 100ml of the mixed solvent. Light green? What for? Light green has a molecular weight of 792.863 CI of 42095 and dissolves in water at 20g per 100 mls and in alcohol at 0.82 g per 100 mls. In Masson's stain a 2% aqueous light green in 1% acetic acid solution is used. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 The real voyage of discovery consists not in seeing new landscapes, but in having new eyes. --Marcel Proust This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From hymclab <@t> hyhc.com Fri Jul 7 07:42:55 2006 From: hymclab <@t> hyhc.com (hymclab) Date: Fri Jul 7 07:35:31 2006 Subject: [Histonet] Disposable blades Message-ID: Ditto. Dawn -----Original Message----- From: Pamela Marcum [mailto:pmarcum@vet.upenn.edu] Sent: Thursday, July 06, 2006 12:41 PM To: crains@wpmpath.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Disposable blades We use the Shandon MX35 Premier + and they are very good. Good life on the edge and more economical than Accu Edge. Pam Marcum UPenn Vet School At 12:37 PM 7/6/2006, crains@wpmpath.com wrote: >In the process of changing to disposable microtome blades. Any >feedback on which brands work best, or which ones to avoid? Thanks. > >Chris Rains >WPM Pathology laboratory >Salina, KS > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dmccaig <@t> ckha.on.ca Fri Jul 7 07:43:07 2006 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Fri Jul 7 07:47:45 2006 Subject: [Histonet] HISTOLOGY COMPUTER SYSTEM Message-ID: Hi We are possibly purchasing a computer system for the histology department and am wondering your opinions on systems that are available and pros/cons for them. Diana McCaig 519-352-6401 (6604) Charge Technologist, Histology Chatham Kent Heath Alliance 80 Grand Ave West Chatham, Ontario N7L 1B7 From Robert.Lott <@t> bhsala.com Fri Jul 7 09:22:25 2006 From: Robert.Lott <@t> bhsala.com (Lott, Robert) Date: Fri Jul 7 09:22:45 2006 Subject: [Histonet] Help Please!! ... with MMA Message-ID: Histonetters, I have been making Polymethylmethacrylate plastic since I have worked here. The idea, as you know, is to embed the bones into a matrix at least as hard as they are, so that they can be cut into 5 micron sections. The trouble I'm having now is that I cannot get the sections to de-plasticize! In the past, we have just always simply put them into xylene overnight, (or even 2x - 20-30 minute changes of acetone when we were in a hurry), and the PMMA would dissolve so that they could be stained. Now (since the first of the summer) the plastic will not dissolve. It's as if I have created a "super-polymer"! The thin section of PMMA just turns to "goo" in any solvent I try! It is extremely warm and humid in my laboratory environment, and I believe this has contributed to the problem! So far, I have tried the following: Xylene, acetone, THF (tetrahydrofuran), plain monomer (MMA), trichloroethelene, 2-methoxyethylacetate, DMF (dimethyformamide), DMSO Methylethylketone, polyethylene glycol, dichloromethane, hexane, chloroform, perchlorethelene (from the dry cleaners), "Goo-gone" (available in the hardware store) I have also tried all of the above with heat and agitation, as well as some combinations of the above. I am at my wit's end! I must find a way to remove the plastic! I tried re-making the PMMA with ALL NEW solutions, that had just been ordered and kept refrigerated. No luck! Same gooey mess....cannot stain them! Does anyone have any ideas about what may be the problem as well as how this may be remedied ? Patty Lott Orthopedic Research UAB Medical Center plott@uab.edu ----------------------------------------- Confidentiality Notice: The information contained in this email message is privileged and confidential information and intended only for the use of the individual or entity named in the address. If you are not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this information is strictly prohibited. If you received this information in error, please notify the sender and delete this information from your computer and retain no copies of any of this information. From TillRenee <@t> uams.edu Fri Jul 7 09:54:58 2006 From: TillRenee <@t> uams.edu (Till, Renee) Date: Fri Jul 7 09:55:27 2006 Subject: [Histonet] target retrieval solution Message-ID: <11F927674DEBDC43B960809A7403C5D201602E4A@MAILPED.ad.uams.edu> Does anyone know exactly what the Target Retrieval solution (S1699) is? I noticed some of the others say Tris, or Citrate, or whatever, but not this one. I was hoping someone would know how to make up some. I am almost out and the person who did the ordering for this project ordered the wrong one ( Tris/EDTA ph9). Dako said either should work for my antibody, but I already had the one optimized to my microwave protocol and the other does not work at the same conditions. I was hoping to avoid all that extra work...lol. Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72002 Office (501)364-2785 Fax (501)364-3161 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From BMolinari <@t> heart.thi.tmc.edu Fri Jul 7 10:57:47 2006 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Jul 7 10:57:51 2006 Subject: [Histonet] michaels fixative Message-ID: Can anyone tell me something about this fixative? Thanks! Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) From TJasper <@t> smdc.org Fri Jul 7 11:09:04 2006 From: TJasper <@t> smdc.org (Jasper, Thomas G.) Date: Fri Jul 7 11:09:23 2006 Subject: [Histonet] HISTOLOGY COMPUTER SYSTEM In-Reply-To: Message-ID: <1A9F2A6C5762524799A816F1F09744CF1E0B0D@SCREECH.ntcampus.smdc.org> Hey Diana, I would consider Tamtron (PowerPath) if I were in your position. I would suggest probably getting 3 or more demonstations from various vendors before making a decision. We've had good luck with Tamtron, however not knowing the scope of your service, etcetera, another system could very well meet your needs. Let me know if you'd like to discuss this in more detail. I'd be happy to relate our experience, you could compare your needs to ours. Good luck, Thomas Jasper AP Supervisor SMDC - Duluth, MN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Diana McCaig Sent: Friday, July 07, 2006 7:43 AM To: Histonet (E-mail) Subject: [Histonet] HISTOLOGY COMPUTER SYSTEM Hi We are possibly purchasing a computer system for the histology department and am wondering your opinions on systems that are available and pros/cons for them. Diana McCaig 519-352-6401 (6604) Charge Technologist, Histology Chatham Kent Heath Alliance 80 Grand Ave West Chatham, Ontario N7L 1B7 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From cpomajzl <@t> cpllabs.com Fri Jul 7 11:37:10 2006 From: cpomajzl <@t> cpllabs.com (Chris Pomajzl) Date: Fri Jul 7 11:31:40 2006 Subject: [Histonet] michaels fixative References: Message-ID: <000c01c6a1e3$99da3360$26fca8c0@CSP> First of all, it is not really a fixative. It is more of a static transport media that minimizes proteolysis. Although, it does "fix" immunoglobulins in the tissue, which is needed for Immunofluorescence. I cannot provide a reference, but I have read that normal saline works as well, if not better, than Michel's sloution. Michel's is good for long-term storage (2-3 days), whereas saline is good for very short periods of time. ----- Original Message ----- From: "Molinari, Betsy" To: Sent: Friday, July 07, 2006 10:57 AM Subject: [Histonet] michaels fixative Can anyone tell me something about this fixative? Thanks! Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vazquezr <@t> ohsu.edu Fri Jul 7 11:40:17 2006 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Jul 7 11:40:44 2006 Subject: [Histonet] michaels fixative Message-ID: Betsy, It is just like Zeus ScientificTransport Medium. You use it when you do not want the tissue fixed. I send it to my clients, to send me tissue for immunofluoresence. Robyn OHSU >>> "Molinari, Betsy" 7/7/2006 8:57 AM >>> Can anyone tell me something about this fixative? Thanks! Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From victor <@t> pathology.washington.edu Fri Jul 7 12:06:13 2006 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Fri Jul 7 12:06:28 2006 Subject: [Histonet] HISTOLOGY COMPUTER SYSTEM In-Reply-To: <1A9F2A6C5762524799A816F1F09744CF1E0B0D@SCREECH.ntcampus.smdc.org> References: <1A9F2A6C5762524799A816F1F09744CF1E0B0D@SCREECH.ntcampus.smdc.org> Message-ID: <44AE9485.6090708@pathology.washington.edu> I would second the vote on PowerPath (IMPAC is the current owner). We are a large academic facility with 3 sites. PowerPath was already installed when I got here, so I have nothing to compare to. It meets are needs and where it is lacking, we have been able to customize it ourselves. Gotta love the open architecture. Victor Jasper, Thomas G. wrote: > Hey Diana, > > I would consider Tamtron (PowerPath) if I were in your position. I would suggest probably getting 3 or more demonstations from various vendors before making a decision. We've had good luck with Tamtron, however not knowing the scope of your service, etcetera, another system could very well meet your needs. > Let me know if you'd like to discuss this in more detail. I'd be happy to relate our experience, you could compare your needs to ours. > Good luck, > Thomas Jasper > AP Supervisor > SMDC - Duluth, MN > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Diana > McCaig > Sent: Friday, July 07, 2006 7:43 AM > To: Histonet (E-mail) > Subject: [Histonet] HISTOLOGY COMPUTER SYSTEM > > > Hi > We are possibly purchasing a computer system for the histology department and am wondering your opinions on systems that are available and pros/cons for them. > > Diana McCaig 519-352-6401 (6604) > Charge Technologist, Histology > Chatham Kent Heath Alliance > 80 Grand Ave West > Chatham, Ontario > N7L 1B7 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. From Charlene.Henry <@t> STJUDE.ORG Fri Jul 7 12:16:25 2006 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Fri Jul 7 12:16:34 2006 Subject: [Histonet] Mordant for PTAH Message-ID: <5CB39BCA5724F349BCB748675C6CA1A2099A1D12@SJMEMXMB02.stjude.sjcrh.local> We are trying to rid the lab of all mercury products and I was wondering what everyone is using as a mordant for the PTAH. We are currently using 4% mercuric chloride and I would like to replace it with another mordant. Thanks, Charlene Henry HT (ASCP), QIHC Histology/Immunohistochemistry Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 From HerberDL <@t> moffitt.usf.edu Fri Jul 7 12:29:06 2006 From: HerberDL <@t> moffitt.usf.edu (Herber, Donna L.) Date: Fri Jul 7 12:29:40 2006 Subject: [Histonet] Successful Gr-1 staining Message-ID: <21E81F01D3A58943AD79F8753AEF65A24A7455@M-EXVMB2.hlm.ad.moffitt.usf.edu> I just wanted to publically thank Gail Callis for her excellent feedback regarding mouse Gr-1 IHC. Needless to say her protocol worked brilliantly, yeilding very specific, intense staining in mouse spleen. Thank you Gail! Donna Donna Herber, Ph.D. Post Doctoral Scholar Immunology (Gabrilovich Laboratory) H. Lee Moffitt Cancer Center and Research Institute 745-6097 ----------------------------------------- ################################################################### ########## This transmission may be confidential or protected from disclosure and is only for review and use by the intended recipient. Access by anyone else is unauthorized. Any unauthorized reader is hereby notified that any review, use, dissemination, disclosure or copying of this information, or any act or omission taken in reliance on it, is prohibited and may be unlawful. If you received this transmission in error, please notify the sender immediately. Thank you. ################################################################### ########## From lblazek <@t> digestivespecialists.com Fri Jul 7 12:36:26 2006 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Fri Jul 7 12:32:40 2006 Subject: [Histonet] HISTOLOGY COMPUTER SYSTEM Message-ID: <6CBA6DC98A079D408C87250591D9DFB80233E0F8@bruexchange.digestivespecialists.com> I'd like to third the vote for PowerPath. I don't use it where I am currently but have used it for the past 6 years from initial install through a major upgrade. It's an excellent system. Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victor Tobias Sent: Friday, July 07, 2006 1:06 PM To: Jasper, Thomas G. Cc: histonet@lists.utsouthwestern.edu; Diana McCaig Subject: Re: [Histonet] HISTOLOGY COMPUTER SYSTEM I would second the vote on PowerPath (IMPAC is the current owner). We are a large academic facility with 3 sites. PowerPath was already installed when I got here, so I have nothing to compare to. It meets are needs and where it is lacking, we have been able to customize it ourselves. Gotta love the open architecture. Victor Jasper, Thomas G. wrote: > Hey Diana, > > I would consider Tamtron (PowerPath) if I were in your position. I would suggest probably getting 3 or more demonstations from various vendors before making a decision. We've had good luck with Tamtron, however not knowing the scope of your service, etcetera, another system could very well meet your needs. > Let me know if you'd like to discuss this in more detail. I'd be happy to relate our experience, you could compare your needs to ours. > Good luck, > Thomas Jasper > AP Supervisor > SMDC - Duluth, MN > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Diana > McCaig > Sent: Friday, July 07, 2006 7:43 AM > To: Histonet (E-mail) > Subject: [Histonet] HISTOLOGY COMPUTER SYSTEM > > > Hi > We are possibly purchasing a computer system for the histology department and am wondering your opinions on systems that are available and pros/cons for them. > > Diana McCaig 519-352-6401 (6604) > Charge Technologist, Histology > Chatham Kent Heath Alliance > 80 Grand Ave West > Chatham, Ontario > N7L 1B7 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charlene.Henry <@t> STJUDE.ORG Fri Jul 7 12:34:45 2006 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Fri Jul 7 12:34:51 2006 Subject: [Histonet] IHC Workload. . Message-ID: <5CB39BCA5724F349BCB748675C6CA1A2099A1D13@SJMEMXMB02.stjude.sjcrh.local> 1. We run approximately 150 to 200 IHC slides (research and clinical) and 5 to 10 ISH slides per day. 2. All of our slides (patient and controls) are cut in Histology and ready for IHC Lab by 8:00 AM 3. We have 2 Benchmark XT instruments and 2 Dako instruments and all of our IHC and ISH protocols are automated. 4. We have 2 technicians that perform these tests. All animal IHC tests are performed by a tech that only performs animal IHC and we have 1 tech that does the clinical IHC and ISH tests. All of my techs are cross-trained in IHC and the IHC rotation is rotated monthly. If IHC tests had to be performed manually, it would take probably 4 techs to complete the workload accurately and in a timely manner. Hope this helps. It will be interesting to see responses from other labs. Charlene Henry HT (ASCP), QIHC Histology/Immunohistochemistry Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer MacDonald Sent: Thursday, July 06, 2006 4:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Workload. . I have a few questions for IHC labs related to workload and staffing. Thank you. 1. How many slides per day? 2. Are slides processed, embedded, and cut by the IHC staff or elsewhere? 3. Automation or manual. If automation what instrument? 4. Number of staff members to perform the workload? How many histotechs? How many lab assistants? Thanks to all who help with this. Jennifer MacDonald Mt. San Antonio College _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Fri Jul 7 12:49:15 2006 From: tkngflght <@t> yahoo.com (Cheryl) Date: Fri Jul 7 12:49:20 2006 Subject: [Histonet] michels fixative In-Reply-To: Message-ID: <20060707174915.24657.qmail@web50902.mail.yahoo.com> Betsy-- You'll need to wash your samples before processing--clearer more vibrant flourescence. Two to three 15 min changes depending on size (we put the kidneys/punch skins) on a rotating blood wheel) with sterile n. saline. Also, be very careful with the outdates on the purchased solutions. It has a short open time as stated by some manufacturers. Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one Tech at a time. 281.883.7704 c 281.852.9457 o admin@fullstaff.org Sign up for the FREE monthly newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. From BMolinari <@t> heart.thi.tmc.edu Fri Jul 7 13:01:05 2006 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Jul 7 13:01:09 2006 Subject: [Histonet] Michels/Thanks Message-ID: A big thank you for all those that responded so quickly to my request. A big help indeed..problem solved. Have a great weekend. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) From RSRICHMOND <@t> aol.com Fri Jul 7 13:08:18 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Jul 7 13:08:24 2006 Subject: [Histonet] Re: Giemsa Stain Message-ID: <552.1f644dc.31dffd12@aol.com> Kim Tournear asks >>I'm in need of a recipe for a giemsa stain using the powder. I also need a recipe for making light green from powder.<< I've never - in more than 40 years - encountered anyone - including an intensely giemsophilic research lab 40 years ago - Wolbach's colophonium rosin and all - who made up their own Giemsa stain solution from the dry powder. It's supposed to be extremely difficult to do. Scrupulous exclusion of water is essential - that applies to stock bottles of the ready-made solution also. Kemlo has given you adequate advice about Light Green SF. - And yes, Kemlo, many labs on this side of the pond have no books. American science education marches on into the new century. Bob Richmond Knoxville TN and Gastonia NC From RSRICHMOND <@t> aol.com Fri Jul 7 13:12:03 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Jul 7 13:12:09 2006 Subject: [Histonet] Michel's transport medium Message-ID: <536.2feb0a6.31dffdf3@aol.com> Michel's transport medium - not a fixative - similar to Zeus transport medium - contains a great deal of ammonium sulfate - used to transport renal biopsy specimens for immunofluorescence - does NOT substitute for RPMI for flow cytometry or cytogenetics. Pronounced mee-SHELL, like the Beatles song. Bob Richmond Knoxville TN and Gastonia NC From doug <@t> ppspath.com Fri Jul 7 13:19:53 2006 From: doug <@t> ppspath.com (Douglas D. Deltour) Date: Fri Jul 7 13:17:07 2006 Subject: [Histonet] Michel's transport medium In-Reply-To: <536.2feb0a6.31dffdf3@aol.com> Message-ID: Let It Be. :) Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of RSRICHMOND@aol.com Sent: Friday, July 07, 2006 2:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Michel's transport medium Michel's transport medium - not a fixative - similar to Zeus transport medium - contains a great deal of ammonium sulfate - used to transport renal biopsy specimens for immunofluorescence - does NOT substitute for RPMI for flow cytometry or cytogenetics. Pronounced mee-SHELL, like the Beatles song. Bob Richmond Knoxville TN and Gastonia NC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Jul 7 13:22:37 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jul 7 13:22:41 2006 Subject: [Histonet] IHC Workload In-Reply-To: Message-ID: <20060707182237.54978.qmail@web61214.mail.yahoo.com> Jennifer: From a survey I finished in Feb./06 (Advance Magazine 3 July/06) I can give you some general information: 1-the average of IHC tests/year in 22 foreign labs = 18,000 2-the average for USA labs (23) = 8,000 [General averages between 300 and 69,000 slides/year for all labs). 3- the difference in IHC workload between foreign and USA labs is significant (P<0.05) 4- usually the slides are cut in the same lab (average 2 hours/day). 5-46% of labs use autostainers (different makes). 6-for total workload = between 1 and 27 HTs (Average = 8; data from 122 labs). 7-lab assistants: between 0 and 7, average = 2 (48 labs). Hope this will help you! Ren? J. Jennifer MacDonald wrote: I have a few questions for IHC labs related to workload and staffing. Thank you. 1. How many slides per day? 2. Are slides processed, embedded, and cut by the IHC staff or elsewhere? 3. Automation or manual. If automation what instrument? 4. Number of staff members to perform the workload? How many histotechs? How many lab assistants? Thanks to all who help with this. Jennifer MacDonald Mt. San Antonio College _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. From rjbuesa <@t> yahoo.com Fri Jul 7 13:26:03 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jul 7 13:26:08 2006 Subject: [Histonet] Mordant for PTAH In-Reply-To: <5CB39BCA5724F349BCB748675C6CA1A2099A1D12@SJMEMXMB02.stjude.sjcrh.local> Message-ID: <20060707182603.99181.qmail@web61220.mail.yahoo.com> Henry: Try using 5% potassium dichromate at 60?C for 30 minutes. It has worked for me. Ren? J. "Henry, Charlene" wrote: We are trying to rid the lab of all mercury products and I was wondering what everyone is using as a mordant for the PTAH. We are currently using 4% mercuric chloride and I would like to replace it with another mordant. Thanks, Charlene Henry HT (ASCP), QIHC Histology/Immunohistochemistry Section Head Department of Pathology St. Jude Children's Research Hospital 901-495-3191 fax 901-495-3100 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. From tkngflght <@t> yahoo.com Fri Jul 7 13:30:34 2006 From: tkngflght <@t> yahoo.com (Cheryl) Date: Fri Jul 7 13:30:40 2006 Subject: [Histonet] microwave processors/sectionable cassettes--vendor responses welcome privately... In-Reply-To: <20060707182237.54978.qmail@web61214.mail.yahoo.com> Message-ID: <20060707183034.82326.qmail@web50914.mail.yahoo.com> Hi all- I'd like to learn more about the various features/benefits/cost per cassette on the various microwave processors including the major improvement since they first came out. I'd also like marketing materials and samples of sectionable cassettes and info on compatiblity with microwave processors. Please call or respond by email or snail mail: Thank you in advance! Cheryl Kerry HT(ASCP) Full Staff Inc. 6315 Red Cedar Ct Kingwood TX 77346 800.756.3309 admin@fullstaff.org Staffing the AP Lab - one tech at a time. From DOOLEEO <@t> shands.ufl.edu Fri Jul 7 14:38:23 2006 From: DOOLEEO <@t> shands.ufl.edu (Elaine Dooley) Date: Fri Jul 7 14:39:01 2006 Subject: [Histonet] BKV vendors Message-ID: Dear Histonetters, What vendors do people use for BK virus antibodies for immunohistochemistry? Elaine Dooley HTL Shands Teaching Hospital Gainesville FL 352-265-0111 ext 7-2117 From la.sebree <@t> hosp.wisc.edu Fri Jul 7 15:00:32 2006 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Fri Jul 7 15:00:39 2006 Subject: [Histonet] BKV vendors Message-ID: Calbiochem Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elaine Dooley Sent: Friday, July 07, 2006 2:38 PM To: Histonet@pathology.swmed.edu Subject: [Histonet] BKV vendors Dear Histonetters, What vendors do people use for BK virus antibodies for immunohistochemistry? Elaine Dooley HTL Shands Teaching Hospital Gainesville FL 352-265-0111 ext 7-2117 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mgdelaware <@t> comcast.net Fri Jul 7 17:06:21 2006 From: mgdelaware <@t> comcast.net (Marian Powers) Date: Fri Jul 7 17:07:35 2006 Subject: [Histonet] HT Practical Tissue Message-ID: <000801c6a211$94e99e80$3227c847@D7XQNX91> Hello all, We are looking for: 1.5 cm in length Cervix( to include ectocervix, epithelium to cover one entire surface. Also 1.5 cm in length Esophagus (not autolyzed, include all layers and epithelium to cover one entire surface. Anyone willing to share some tissue for two techs working on their HT practical? Thanks in advance, Marian Powers, HT(ASCP) Technical Manager Doctors Pathology Services 882 Walker Rd. Dover, DE 19934 (302) 677-0000 From dcd710 <@t> charter.net Fri Jul 7 20:35:24 2006 From: dcd710 <@t> charter.net (dcd710@charter.net) Date: Fri Jul 7 20:35:30 2006 Subject: [Histonet] Tissue Processor Message-ID: <1031047953.1152322524527.JavaMail.root@fepweb12> Histonetters, We are a small laboratory with a volume of approximately 5,000 cases annually consisting of GI bxs, breast, colon, placentas, etc. We will be buying a new tissue processor this year and I'd like your input. We will only use one processor so we need something that will be compatable with all tissue. Thanks for your help ! From rjbuesa <@t> yahoo.com Sat Jul 8 09:01:25 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Jul 8 09:01:30 2006 Subject: [Histonet] Tissue Processor In-Reply-To: <1031047953.1152322524527.JavaMail.root@fepweb12> Message-ID: <20060708140125.63200.qmail@web61213.mail.yahoo.com> If you want to buy a tissue processor along with "peace of mind" buy a VIP from SakuraFinetek. With 5,000 cases/year you probably have an average of 20,000 blocks/year at a rate of approx. less than 100 blocks/day (Mon-Fri). For that volume you can buy one of the small ones (VIP2000 or VIP200 or whatever they are called now!). Do not risk workflow by buying a cheaper and much less reliable tissue processor. Hope this will help you! Ren? J. dcd710@charter.net wrote: Histonetters, We are a small laboratory with a volume of approximately 5,000 cases annually consisting of GI bxs, breast, colon, placentas, etc. We will be buying a new tissue processor this year and I'd like your input. We will only use one processor so we need something that will be compatable with all tissue. Thanks for your help ! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Sneak preview the all-new Yahoo.com. It's not radically different. Just radically better. From RSRICHMOND <@t> aol.com Sat Jul 8 13:10:50 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Sat Jul 8 13:11:01 2006 Subject: [Histonet] Re: Mordant for PTAH Message-ID: <431.473e7b6.31e14f2a@aol.com> Charlene Henry HT (ASCP), QIHC at St. Jude Children's Research Hospital [Memphis, Tennessee] asks: >>We are trying to rid the lab of all mercury products and I was wondering what everyone is using as a mordant for the PTAH. We are currently using 4% mercuric chloride and I would like to replace it with another mordant.<< and Rene J Buesa replies>>Try using 5% potassium dichromate at 60?C for 30 minutes. It has worked for me.<< But then you have to dispose of chromium, which is as difficult as mercury to get hauled away, I think. But what does anyone use phosphotungstic acid hematoxylin (PTAH) for nowadays anyway? Forty years ago it was of some use for staining muscle striations (in suspected rhabdomyosarcomas) and astrocytes - if the tissue had been fixed in Zenker/Helly (mercury, chromium, and formaldehyde). I recall it being said that the chief virtue of PTAH was that it took overnight to do the stain, and that gave you time enough to try to identify an unusual tumor. (It also took three months to make PTAH - you didn't add an oxidant, just put it on the back porch like sun tea.) According to Giuseppe Verdi or his librettist, Aida praised it to the skies ("Omnipotente Ptah" - sorry about that), but I think PTAH's fortunes have been in decline ever since. Bob Richmond Knoxville TN and Gastonia NC From dianaschleicher <@t> yahoo.com Sat Jul 8 14:30:14 2006 From: dianaschleicher <@t> yahoo.com (Diana Schleicher) Date: Sat Jul 8 14:30:22 2006 Subject: [Histonet] Special stains and IHC for Gastric Cancer Message-ID: <20060708193014.94230.qmail@web53503.mail.yahoo.com> I am currently a histotech student. I am writing a paper and preparing a power point presentation on the subject of Gastric Cancer. I would like to find out what tests labs are performing for this disease. Thank you. Diana --------------------------------- Sneak preview the all-new Yahoo.com. It's not radically different. Just radically better. From muddymoo <@t> gmail.com Sat Jul 8 18:43:31 2006 From: muddymoo <@t> gmail.com (Alan Bishop) Date: Sat Jul 8 18:43:37 2006 Subject: [Histonet] IHC Workload In-Reply-To: <20060707182237.54978.qmail@web61214.mail.yahoo.com> References: <20060707182237.54978.qmail@web61214.mail.yahoo.com> Message-ID: Some interesting stats there. Our lab does around 20,000 IHC slides a year. Most blocks already processed and embedded from us doing the routine histology but the daily workload of recutting, adding controls and the whole IHC process is done by one person! All of our workload is done by hand at the moment and all achieved in less than a standard working day - usually all issued to the paths by mid afternoon. >From the survey I should probably think about getting some more staff for the IHC :-) Cheers Alan On 08/07/06, Rene J Buesa wrote: > > Jennifer: > From a survey I finished in Feb./06 (Advance Magazine 3 July/06) I can > give you some general information: > 1-the average of IHC tests/year in 22 foreign labs = 18,000 > 2-the average for USA labs (23) = 8,000 [General averages between 300 > and 69,000 slides/year for all labs). > 3- the difference in IHC workload between foreign and USA labs is > significant (P<0.05) > 4- usually the slides are cut in the same lab (average 2 hours/day). > 5-46% of labs use autostainers (different makes). > 6-for total workload = between 1 and 27 HTs (Average = 8; data from 122 > labs). > 7-lab assistants: between 0 and 7, average = 2 (48 labs). > Hope this will help you! > Ren? J. > > > Jennifer MacDonald wrote: > I have a few questions for IHC labs related to workload and staffing. > Thank you. > 1. How many slides per day? > 2. Are slides processed, embedded, and cut by the IHC staff or elsewhere? > 3. Automation or manual. > If automation what instrument? > 4. Number of staff members to perform the workload? > How many histotechs? How many lab assistants? > > Thanks to all who help with this. > Jennifer MacDonald > Mt. San Antonio College > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great > rates starting at 1?/min. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From ROrr <@t> enh.org Sun Jul 9 10:09:15 2006 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Sun Jul 9 10:09:20 2006 Subject: [Histonet] RE: IHC workload Message-ID: Hi Jennifer, Our IHC lab is separate from the Routine Histology lab. We do not rotate techs into the IHC lab here. We have a Technical Specialist (HTL, QIHC) who is responsible for the IHC lab. He works 7:30am-4pm. Then we have a Tech (HT,) who comes in from 10:30am -7:00pm (she actually LIKES THAT SHIFT!) So we have 2 people covering almost 12hours. This helps when the Docs come in late in the afternoon with IHC requests; we have coverage until long after they are gone to cut everything that comes in. Overnight in the oven is a luxury we love having. On occasion though, we do run slides the same day. Lately we've been looking at the LEAN 6-sigma set up where instead of one large batch of slides once a day, we're processing several smaller runs to cut down on total run processing time. I'd be glad to discuss this further in a separate email, if you're interested. We run approx. 100-150 slides/day with a Ventana Benchmark and a Biocare Nemesis. The Autostainer platform of the Nemesis is great for working up research projects that require larger numbers of slides. To finish answering your questions, the Histology lab cuts the H/E slides. Docs read the H/E and order IHC. We get the orders and then hunt down the blocks (some Docs are trained to submit the blocks) and cut our own IHC. We have a cassette holder re-alignment instrument that keeps all of the microtomes lined up, so blocks can be cut from any microtome. There are always cases that arise where an FNA core biopsy is submitted and the IHC lab cuts the H/E and takes unstained slides immediately. IHC lab does not embed. I recommend that the person leading the IHC section have a propensity for running these stains. It would be advantageous for this person to have a keen interest in keeping the lab updated with new antibodies. It kind of depends, on your Pathologists. Our lab is part of a teaching hospital with 13 Pathologists and a dozen Pathology residents each doing separate research projects. We are always working on a poster or abstract for one of them. So in our IHC lab, we need a progressive leader who is interested in working with the consistency of change...someone who has the experience and the progressive attitude to research new antibodies and juggle research and clinical assignments on a very regular basis. There are many labs that require a menu of 20-30 antibodies to be run in a consistent routine, so in this case the IHC personnel requirements might be a bit different.(My opinion) Hope this helps, Becky Becky Orr CLA,HT(ASCP)QIHC Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 Message: 15 Date: Thu, 6 Jul 2006 14:43:18 -0700 From: Jennifer MacDonald Subject: [Histonet] IHC Workload To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" I have a few questions for IHC labs related to workload and staffing. Thank you. 1. How many slides per day? 2. Are slides processed, embedded, and cut by the IHC staff or elsewhere? 3. Automation or manual. If automation what instrument? 4. Number of staff members to perform the workload? How many histotechs? How many lab assistants? Thanks to all who help with this. Jennifer MacDonald Mt. San Antonio College ------------------------------ From H.I.Grabsch <@t> leeds.ac.uk Sun Jul 9 12:10:12 2006 From: H.I.Grabsch <@t> leeds.ac.uk (Heike Grabsch) Date: Sun Jul 9 12:12:47 2006 Subject: [Histonet] gastric cancer References: <200607091707.k69H7Vxm021554@mserv2.leeds.ac.uk> Message-ID: Why do you want to do a special test for gastric cancer? If you want to diagnose it on a resection speciment, you don't need one. But may be you have a particular question in mind, such as idnetifying diffuse type gastric cancer cells in a biopsy or soemthing like that or do you want to know the CK combination for the differential diagnosis? If you are more specific, I may be able to help Heike Dr Heike Grabsch, MRCPath MD Gastrointestinal Cancer Research Group The Leeds Institute of Molecular Medicine Section of Pathology and Tumour Biology Wellcome Trust Brenner Bldg, Level 4 St James's University Hospital Beckett Street Leeds LS9 7TF England ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of histonet-request@lists.utsouthwestern.edu Sent: Sun 09/07/2006 18:07 To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 32, Issue 9 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Mordant for PTAH (RSRICHMOND@aol.com) 2. Special stains and IHC for Gastric Cancer (Diana Schleicher) 3. Re: IHC Workload (Alan Bishop) 4. RE: IHC workload (Orr, Rebecca) ---------------------------------------------------------------------- Message: 1 Date: Sat, 8 Jul 2006 14:10:50 EDT From: RSRICHMOND@aol.com Subject: [Histonet] Re: Mordant for PTAH To: histonet@lists.utsouthwestern.edu Message-ID: <431.473e7b6.31e14f2a@aol.com> Content-Type: text/plain; charset="ISO-8859-1" Charlene Henry HT (ASCP), QIHC at St. Jude Children's Research Hospital [Memphis, Tennessee] asks: >>We are trying to rid the lab of all mercury products and I was wondering what everyone is using as a mordant for the PTAH. We are currently using 4% mercuric chloride and I would like to replace it with another mordant.<< and Rene J Buesa replies>>Try using 5% potassium dichromate at 60?C for 30 minutes. It has worked for me.<< But then you have to dispose of chromium, which is as difficult as mercury to get hauled away, I think. But what does anyone use phosphotungstic acid hematoxylin (PTAH) for nowadays anyway? Forty years ago it was of some use for staining muscle striations (in suspected rhabdomyosarcomas) and astrocytes - if the tissue had been fixed in Zenker/Helly (mercury, chromium, and formaldehyde). I recall it being said that the chief virtue of PTAH was that it took overnight to do the stain, and that gave you time enough to try to identify an unusual tumor. (It also took three months to make PTAH - you didn't add an oxidant, just put it on the back porch like sun tea.) According to Giuseppe Verdi or his librettist, Aida praised it to the skies ("Omnipotente Ptah" - sorry about that), but I think PTAH's fortunes have been in decline ever since. Bob Richmond Knoxville TN and Gastonia NC ------------------------------ Message: 2 Date: Sat, 8 Jul 2006 12:30:14 -0700 (PDT) From: Diana Schleicher Subject: [Histonet] Special stains and IHC for Gastric Cancer To: histonet@lists.utsouthwestern.edu Message-ID: <20060708193014.94230.qmail@web53503.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I am currently a histotech student. I am writing a paper and preparing a power point presentation on the subject of Gastric Cancer. I would like to find out what tests labs are performing for this disease. Thank you. Diana --------------------------------- Sneak preview the all-new Yahoo.com. It's not radically different. Just radically better. ------------------------------ Message: 3 Date: Sun, 9 Jul 2006 11:43:31 +1200 From: "Alan Bishop" Subject: Re: [Histonet] IHC Workload To: "Rene J Buesa" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1; format=flowed Some interesting stats there. Our lab does around 20,000 IHC slides a year. Most blocks already processed and embedded from us doing the routine histology but the daily workload of recutting, adding controls and the whole IHC process is done by one person! All of our workload is done by hand at the moment and all achieved in less than a standard working day - usually all issued to the paths by mid afternoon. >From the survey I should probably think about getting some more staff for the IHC :-) Cheers Alan On 08/07/06, Rene J Buesa wrote: > > Jennifer: > From a survey I finished in Feb./06 (Advance Magazine 3 July/06) I can > give you some general information: > 1-the average of IHC tests/year in 22 foreign labs = 18,000 > 2-the average for USA labs (23) = 8,000 [General averages between 300 > and 69,000 slides/year for all labs). > 3- the difference in IHC workload between foreign and USA labs is > significant (P<0.05) > 4- usually the slides are cut in the same lab (average 2 hours/day). > 5-46% of labs use autostainers (different makes). > 6-for total workload = between 1 and 27 HTs (Average = 8; data from 122 > labs). > 7-lab assistants: between 0 and 7, average = 2 (48 labs). > Hope this will help you! > Ren? J. > > > Jennifer MacDonald wrote: > I have a few questions for IHC labs related to workload and staffing. > Thank you. > 1. How many slides per day? > 2. Are slides processed, embedded, and cut by the IHC staff or elsewhere? > 3. Automation or manual. > If automation what instrument? > 4. Number of staff members to perform the workload? > How many histotechs? How many lab assistants? > > Thanks to all who help with this. > Jennifer MacDonald > Mt. San Antonio College > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great > rates starting at 1?/min. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 4 Date: Sun, 9 Jul 2006 10:09:15 -0500 From: "Orr, Rebecca" Subject: [Histonet] RE: IHC workload To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Jennifer, Our IHC lab is separate from the Routine Histology lab. We do not rotate techs into the IHC lab here. We have a Technical Specialist (HTL, QIHC) who is responsible for the IHC lab. He works 7:30am-4pm. Then we have a Tech (HT,) who comes in from 10:30am -7:00pm (she actually LIKES THAT SHIFT!) So we have 2 people covering almost 12hours. This helps when the Docs come in late in the afternoon with IHC requests; we have coverage until long after they are gone to cut everything that comes in. Overnight in the oven is a luxury we love having. On occasion though, we do run slides the same day. Lately we've been looking at the LEAN 6-sigma set up where instead of one large batch of slides once a day, we're processing several smaller runs to cut down on total run processing time. I'd be glad to discuss this further in a separate email, if you're interested. We run approx. 100-150 slides/day with a Ventana Benchmark and a Biocare Nemesis. The Autostainer platform of the Nemesis is great for working up research projects that require larger numbers of slides. To finish answering your questions, the Histology lab cuts the H/E slides. Docs read the H/E and order IHC. We get the orders and then hunt down the blocks (some Docs are trained to submit the blocks) and cut our own IHC. We have a cassette holder re-alignment instrument that keeps all of the microtomes lined up, so blocks can be cut from any microtome. There are always cases that arise where an FNA core biopsy is submitted and the IHC lab cuts the H/E and takes unstained slides immediately. IHC lab does not embed. I recommend that the person leading the IHC section have a propensity for running these stains. It would be advantageous for this person to have a keen interest in keeping the lab updated with new antibodies. It kind of depends, on your Pathologists. Our lab is part of a teaching hospital with 13 Pathologists and a dozen Pathology residents each doing separate research projects. We are always working on a poster or abstract for one of them. So in our IHC lab, we need a progressive leader who is interested in working with the consistency of change...someone who has the experience and the progressive attitude to research new antibodies and juggle research and clinical assignments on a very regular basis. There are many labs that require a menu of 20-30 antibodies to be run in a consistent routine, so in this case the IHC personnel requirements might be a bit different.(My opinion) Hope this helps, Becky Becky Orr CLA,HT(ASCP)QIHC Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 Message: 15 Date: Thu, 6 Jul 2006 14:43:18 -0700 From: Jennifer MacDonald Subject: [Histonet] IHC Workload To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" I have a few questions for IHC labs related to workload and staffing. Thank you. 1. How many slides per day? 2. Are slides processed, embedded, and cut by the IHC staff or elsewhere? 3. Automation or manual. If automation what instrument? 4. Number of staff members to perform the workload? How many histotechs? How many lab assistants? Thanks to all who help with this. Jennifer MacDonald Mt. San Antonio College ------------------------------ ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 32, Issue 9 *************************************** From Shirley_PHUA <@t> hsa.gov.sg Sun Jul 9 13:10:28 2006 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Sun Jul 9 13:10:39 2006 Subject: [Histonet] Shirley is away : 10 July 2006 (Monday) Message-ID: I will be out of the office from 10/07/2006 to 10/07/2006. I will return on 11 July 2006 (Tuesday). Pathologists : I will process your requests when I return. Otherwise, if urgent, please contact Henry. From djmr55 <@t> hotmail.com Sun Jul 9 18:42:18 2006 From: djmr55 <@t> hotmail.com (donna rossi) Date: Sun Jul 9 18:42:28 2006 Subject: [Histonet] Cryostat disinfection Message-ID: Here we go again! According to CAP we are to be shutting down the cryostat and disinfecting it every week for cryostats that are used daily. How often should it be shut down if it is used perhaps once a week? Do you determine this by number of frozens being done or type of tissue being cut or just pick a time frame? We are a small hospital and this will impact our work flow but if CAP says it must be so thennnnnnnnnnnnnn. Your help is greatly appreciated. Donna , Sharon Reg Health System From gillian.2.brown <@t> gsk.com Mon Jul 10 02:52:43 2006 From: gillian.2.brown <@t> gsk.com (gillian.2.brown@gsk.com) Date: Mon Jul 10 02:51:31 2006 Subject: [Histonet] Re: Giemsa Stain In-Reply-To: <552.1f644dc.31dffd12@aol.com> Message-ID: Hi, Guess no one told me how hard it is as I do make my own. We do have very ancient stocks so maybe I wont be able to once the components have gone. Regards Gill Brown RSRICHMOND@aol.com Sent by: histonet-bounces@lists.utsouthwestern.edu 07-Jul-2006 19:08 To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Re: Giemsa Stain Kim Tournear asks >>I'm in need of a recipe for a giemsa stain using the powder. I also need a recipe for making light green from powder.<< I've never - in more than 40 years - encountered anyone - including an intensely giemsophilic research lab 40 years ago - Wolbach's colophonium rosin and all - who made up their own Giemsa stain solution from the dry powder. It's supposed to be extremely difficult to do. Scrupulous exclusion of water is essential - that applies to stock bottles of the ready-made solution also. Kemlo has given you adequate advice about Light Green SF. - And yes, Kemlo, many labs on this side of the pond have no books. American science education marches on into the new century. Bob Richmond Knoxville TN and Gastonia NC _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Julie.Sanders <@t> va.gov Mon Jul 10 06:36:30 2006 From: Julie.Sanders <@t> va.gov (Sanders, Julie, VHACIN) Date: Mon Jul 10 06:36:46 2006 Subject: [Histonet] RE: Histonet Digest, Vol 32, Issue 9 In-Reply-To: <526i4s$oqqvn@mtadal1.dal.net.va.gov> Message-ID: <2D4ACE41DEFE93428F23D77988EFBCB50E601F@VHAV10MSGA2.v10.med.va.gov> I have a few questions for IHC labs related to workload and staffing. Thank you. 1. How many slides per day? 2. Are slides processed, embedded, and cut by the IHC staff or elsewhere? 3. Automation or manual. If automation what instrument? 4. Number of staff members to perform the workload? How many histotechs? How many lab assistants? Thanks to all who help with this. Jennifer MacDonald Mt. San Antonio College Jennifer, at the VAMC Cincinnati we run between 10-30 slides per day for IHC. The blocks are embedded and cut by the Histology staff. We have a Ventana Benchmark which gives us the option of running overnight stains since we only have one shift, this works great for us. There's only 3 of us, myself and 2 techs, so we all perform IHC. Our TAT is usually 24 hours or less, normally the docs get their IHC's on the same day ordered if they order them before 12 noon. If it is a longer procedure (TTF-1, etc.) we run these overnight. Hope this helps. Julie From rjbuesa <@t> yahoo.com Mon Jul 10 07:45:01 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Jul 10 07:45:05 2006 Subject: [Histonet] Cryostat disinfection In-Reply-To: Message-ID: <20060710124501.443.qmail@web61212.mail.yahoo.com> Donna: Regardless of the amount of frozen cases, the fundamental criteria is the type of specimen. Do you cryosection TB cases or any which are a potential contamination source? If so you should disinfect the cryostat after each one of those cases. CAP recommends shutting down and cleaning weekly because the probability of having those types of cases on a weekly basis. Being a small facility I think that you should prepare a schedule based on usage of the cryostat or even better, ask CAP if they have some "latitude" regarding small facilities. CAP is always willing to answer. I hope this will help. Ren? J. donna rossi wrote: Here we go again! According to CAP we are to be shutting down the cryostat and disinfecting it every week for cryostats that are used daily. How often should it be shut down if it is used perhaps once a week? Do you determine this by number of frozens being done or type of tissue being cut or just pick a time frame? We are a small hospital and this will impact our work flow but if CAP says it must be so thennnnnnnnnnnnnn. Your help is greatly appreciated. Donna , Sharon Reg Health System _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Why keep checking for Mail? The all-new Yahoo! Mail Beta shows you when there are new messages. From JPCOLEMA <@t> sentara.com Mon Jul 10 07:50:14 2006 From: JPCOLEMA <@t> sentara.com (JOHN P COLEMAN) Date: Mon Jul 10 07:50:37 2006 Subject: [Histonet] Re: Histonet Digest, Vol 32, Issue 8 In-Reply-To: <200607081705.k68H52l22639@sdclin01.sdc.sentara.com> References: <200607081705.k68H52l22639@sdclin01.sdc.sentara.com> Message-ID: PTAH mordant- we use bouin's and it gives good results. IHC staffing/workload. I am the Senior tech for a local reference lab doing IHC for 8 hospitals, our load is about 500 slides per day. They are cut by the IHC staff (me and one other tech). We have 5 Biogenex i6000's, we also do manual IF, Muscle biopsy histochemical stains and Bone Marrow histochemical stains. Short answer- 500 slides a day, 2 techs, no ancillary support, 5 biogenex i6000s John P.J. Coleman HT(ASCP)QIHC Interim Technical Clinical Specialist Anatomic Pathology Sentara Laboratory Services Cell/Voicemail(757)335-2159 pager: (757)456-6695 From sunay <@t> balikesir.edu.tr Mon Jul 10 08:15:45 2006 From: sunay <@t> balikesir.edu.tr (Fatma Bahar SUNAY) Date: Mon Jul 10 08:17:17 2006 Subject: [Histonet] CA IX Message-ID: <20060710131216.M89620@balikesir.edu.tr> Hi everybody, I am looking for a reliable protocol for IHC staining of CA IX in HeLa cell cultures. Thanks a lot. Dr. Bahar Sunay Balikesir University Turkey -- Open WebMail Project (http://openwebmail.org) From vanann702 <@t> skmc.gov.ae Mon Jul 10 08:21:53 2006 From: vanann702 <@t> skmc.gov.ae (Anne Van Binsbergen) Date: Mon Jul 10 08:18:26 2006 Subject: [Histonet] Cryostat disinfection Message-ID: Surely universal precautions apply - all specimens should be treated the same - fresh specimens are all potentially hazardous. Common sense should prevail. In an HIV/TB infested country - where I come from - we had 'panic stations' if we knew the status of the patient(HIV/TB +ve), and everyone donned as much protective gear as they could find!!! - but everyone seemed to forget the first few frozens of the day, where we did not know the HIV/TB status - did that make those specimens less infectious/dangerous??? NO!!! If you have only one cryostat and have frequent frozens you need to take a slightly different approach - educate your surgeons and pathologists - do imprints and smears where possible - switching off, defrosting and cleaning a cryostat will cripple this sort of lab. In my current lab I have 3 cryostats and few frozens and (lucky for me) have relatively 'safe' patients, so I can afford to defrost on a rotational basis. Not everyone is as fortunate. Just my opinion Annieinarabia -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Monday, July 10, 2006 4:45 PM To: donna rossi; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Cryostat disinfection Donna: Regardless of the amount of frozen cases, the fundamental criteria is the type of specimen. Do you cryosection TB cases or any which are a potential contamination source? If so you should disinfect the cryostat after each one of those cases. CAP recommends shutting down and cleaning weekly because the probability of having those types of cases on a weekly basis. Being a small facility I think that you should prepare a schedule based on usage of the cryostat or even better, ask CAP if they have some "latitude" regarding small facilities. CAP is always willing to answer. I hope this will help. Ren? J. donna rossi wrote: Here we go again! According to CAP we are to be shutting down the cryostat and disinfecting it every week for cryostats that are used daily. How often should it be shut down if it is used perhaps once a week? Do you determine this by number of frozens being done or type of tissue being cut or just pick a time frame? We are a small hospital and this will impact our work flow but if CAP says it must be so thennnnnnnnnnnnnn. Your help is greatly appreciated. Donna , Sharon Reg Health System _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Why keep checking for Mail? The all-new Yahoo! Mail Beta shows you when there are new messages. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Mon Jul 10 08:23:38 2006 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Mon Jul 10 08:23:53 2006 Subject: [Histonet] Re: Histonet Digest, Vol 32, Issue 8 Message-ID: John, All I can say is...WOW. That is an UNREAL amount of work output. I hope you are paid handsomely for this almost superhuman effort. Well Done, Glen Dawson IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of JOHN P COLEMAN Sent: Monday, July 10, 2006 6:50 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 32, Issue 8 PTAH mordant- we use bouin's and it gives good results. IHC staffing/workload. I am the Senior tech for a local reference lab doing IHC for 8 hospitals, our load is about 500 slides per day. They are cut by the IHC staff (me and one other tech). We have 5 Biogenex i6000's, we also do manual IF, Muscle biopsy histochemical stains and Bone Marrow histochemical stains. Short answer- 500 slides a day, 2 techs, no ancillary support, 5 biogenex i6000s John P.J. Coleman HT(ASCP)QIHC Interim Technical Clinical Specialist Anatomic Pathology Sentara Laboratory Services Cell/Voicemail(757)335-2159 pager: (757)456-6695 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cwscouten <@t> myneurolab.com Mon Jul 10 09:07:33 2006 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Mon Jul 10 09:07:54 2006 Subject: [Histonet] RE:decontamination. Message-ID: <5784D843593D874C93E9BADCB87342AB01307224@tpiserver03.Coretech-holdings.com> I don't have an answer, but will post this to the histonet. You should consider subscribing. I assume you are not presently in the market for a self decontamininating crystat that cycle itself every night and be ready in the morning? Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: djmr55@hotmail.com [mailto:djmr55@hotmail.com] Sent: Saturday, July 08, 2006 1:57 PM To: Charles Scouten Cc: Doug Martin; Drew N. Mehta; Charles Scouten Subject: myNeuroLab Question Subject:Cryostat Decontamination Question:I work in a small hospital histology lab. Until recently we used chlorhexidine digluconate in 70% ETOh to decontaminate our cryostat at -14C. CAP now says we need to disinfect with 70% and also decontaminate with a tuberculocidal at an interval appropriate to our facility. How do you determine what is appropriate? Number of routine cases cut on a cryostat? We have never had anything that is suspected of T.B., or HIV being cut on our cryostat. Is decontaminating 1 every 3 months okay or once a year? We need to shut this thing down over a weekend and that is another probelem with OR scheduling and no backup instrument . HElP!! Donna Rossi (ASCP) Histosupervisor Sharon Regional Health System From brian.chelack <@t> usask.ca Mon Jul 10 10:08:26 2006 From: brian.chelack <@t> usask.ca (Brian Chelack) Date: Mon Jul 10 10:06:55 2006 Subject: [Histonet] Re: IHC workload Message-ID: <002a01c6a432$b23d9c20$0f13e980@PDS04> Some great information here, but I am wondering if you have any data pertaining to the number of slides stained per FTE (full time equivalent) per year. Another bit of information that would be useful is scope of staining performed by the average lab; that is, how many different stains are in their repertoire? The workload in a lab that runs 50,000 of the same stain is entirely different from a lab that runs 500 slides of each of 100 different stains. Finally, how responsible are the techs for examination of the slides. Do they simply run the stains and ship everything out for the pathologists, do they examine the controls only, or do they run an initial visual screening of the cases? Each of these options changes the workload for the techs and the pathologists dramatically. Thanks, Brian Chelack Prairie Diagnostic Services 52 Campus Drive Saskatoon, SK S7N 5B4 306-966-7241 From clarinjc <@t> hotmail.com Mon Jul 10 10:52:09 2006 From: clarinjc <@t> hotmail.com (Judy Choi) Date: Mon Jul 10 10:52:18 2006 Subject: [Histonet] Troubleshoot on mounting tissue Message-ID: Hi, This is my first time using this listserve, so I hope this works. I have a question about mounting tissues (PFA perfused rat brain tissues to be specific). I processed them for IHC using ABC complex system and DAB and used deionized water as my mounting medium. However, when I put the tissue in water, it swells tremendously and easily becomes wrinkled. I think as a consequence of the swelling, there are holes throughout my tissues. (I didn't use hydrogen peroxide until DAB, and it was at an extremely low concentration of less than 0.1%.) Anyways, is my hypothesis plausible (that mounting my tissue in DI water can cause tissues to swell and create holes)? If so, does anyone know how I can eliminate this problem? Or can it be another factor (bad cryoprotectant, bad perfusion, bad buffer)? I appreciate any feedback I can get. Thank you! Judy From chitnis.c <@t> neu.edu Mon Jul 10 13:12:59 2006 From: chitnis.c <@t> neu.edu (Chinmay Chitnis) Date: Mon Jul 10 13:13:07 2006 Subject: [Histonet] Re: Histonet Digest, Vol 32, Issue 10 Message-ID: <8263085.1152555179321.JavaMail.chitnis.c@neu.edu> hi Can anyone suggest me a source for MMP-1 for bovine collagen? Thanks Chinmay histonet-request@lists.utsouthwestern.edu wrote: >Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > >To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > >You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > >When replying, please edit your Subject line so it is more specific >than "Re: Contents of Histonet digest..." > > >Today's Topics: > > 1. gastric cancer (Heike Grabsch) > 2. Shirley is away : 10 July 2006 (Monday) (Shirley PHUA) > 3. Cryostat disinfection (donna rossi) > 4. Re: Re: Giemsa Stain (gillian.2.brown@gsk.com) > 5. RE: Histonet Digest, Vol 32, Issue 9 (Sanders, Julie, VHACIN) > 6. Re: Cryostat disinfection (Rene J Buesa) > 7. Re: Histonet Digest, Vol 32, Issue 8 (JOHN P COLEMAN) > 8. CA IX (Fatma Bahar SUNAY) > 9. RE: Cryostat disinfection (Anne Van Binsbergen) > 10. RE: Re: Histonet Digest, Vol 32, Issue 8 (Dawson, Glen) > 11. RE:decontamination. (Charles Scouten) > 12. Re: IHC workload (Brian Chelack) > 13. Troubleshoot on mounting tissue (Judy Choi) > > >---------------------------------------------------------------------- > >Message: 1 >Date: Sun, 9 Jul 2006 18:10:12 +0100 >From: "Heike Grabsch" >Subject: [Histonet] gastric cancer >To: >Message-ID: > >Content-Type: text/plain; charset="iso-8859-1" > >Why do you want to do a special test for gastric cancer? If you want to diagnose it on a resection speciment, you don't need one. But may be you have a particular question in mind, such as idnetifying diffuse type gastric cancer cells in a biopsy or soemthing like that or do you want to know the CK combination for the differential diagnosis? If you are more specific, I may be able to help >Heike > >Dr Heike Grabsch, MRCPath MD >Gastrointestinal Cancer Research Group >The Leeds Institute of Molecular Medicine >Section of Pathology and Tumour Biology >Wellcome Trust Brenner Bldg, Level 4 >St James's University Hospital >Beckett Street >Leeds >LS9 7TF >England > > > >________________________________ > >From: histonet-bounces@lists.utsouthwestern.edu on behalf of histonet-request@lists.utsouthwestern.edu >Sent: Sun 09/07/2006 18:07 >To: histonet@lists.utsouthwestern.edu >Subject: Histonet Digest, Vol 32, Issue 9 > > > >Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > >To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > >You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > >When replying, please edit your Subject line so it is more specific >than "Re: Contents of Histonet digest..." > > >Today's Topics: > > 1. Re: Mordant for PTAH (RSRICHMOND@aol.com) > 2. Special stains and IHC for Gastric Cancer (Diana Schleicher) > 3. Re: IHC Workload (Alan Bishop) > 4. RE: IHC workload (Orr, Rebecca) > > >---------------------------------------------------------------------- > >Message: 1 >Date: Sat, 8 Jul 2006 14:10:50 EDT >From: RSRICHMOND@aol.com >Subject: [Histonet] Re: Mordant for PTAH >To: histonet@lists.utsouthwestern.edu >Message-ID: <431.473e7b6.31e14f2a@aol.com> >Content-Type: text/plain; charset="ISO-8859-1" > >Charlene Henry HT (ASCP), QIHC at St. Jude Children's Research Hospital >[Memphis, Tennessee] asks: >>>We are trying to rid the lab of all mercury products and I was wondering >what everyone is using as a mordant for the PTAH. We are currently using 4% >mercuric chloride and I would like to replace it with another mordant.<< and Rene >J Buesa replies>>Try using 5% potassium dichromate at 60?C for 30 minutes. It >has worked for me.<< > >But then you have to dispose of chromium, which is as difficult as mercury to >get hauled away, I think. > >But what does anyone use phosphotungstic acid hematoxylin (PTAH) for nowadays >anyway? Forty years ago it was of some use for staining muscle striations (in >suspected rhabdomyosarcomas) and astrocytes - if the tissue had been fixed in >Zenker/Helly (mercury, chromium, and formaldehyde). I recall it being said >that the chief virtue of PTAH was that it took overnight to do the stain, and >that gave you time enough to try to identify an unusual tumor. (It also took >three months to make PTAH - you didn't add an oxidant, just put it on the back >porch like sun tea.) > >According to Giuseppe Verdi or his librettist, Aida praised it to the skies >("Omnipotente Ptah" - sorry about that), but I think PTAH's fortunes have been >in decline ever since. > >Bob Richmond >Knoxville TN and Gastonia NC > > >------------------------------ > >Message: 2 >Date: Sat, 8 Jul 2006 12:30:14 -0700 (PDT) >From: Diana Schleicher >Subject: [Histonet] Special stains and IHC for Gastric Cancer >To: histonet@lists.utsouthwestern.edu >Message-ID: <20060708193014.94230.qmail@web53503.mail.yahoo.com> >Content-Type: text/plain; charset=iso-8859-1 > >I am currently a histotech student. I am writing a paper and preparing a power point presentation on the subject of Gastric Cancer. I would like to find out what tests labs are performing for this disease. Thank you. > >Diana > > >--------------------------------- >Sneak preview the all-new Yahoo.com. It's not radically different. Just radically better. > >------------------------------ > >Message: 3 >Date: Sun, 9 Jul 2006 11:43:31 +1200 >From: "Alan Bishop" >Subject: Re: [Histonet] IHC Workload >To: "Rene J Buesa" >Cc: histonet@lists.utsouthwestern.edu >Message-ID: > >Content-Type: text/plain; charset=ISO-8859-1; format=flowed > >Some interesting stats there. > >Our lab does around 20,000 IHC slides a year. Most blocks already processed >and embedded from us doing the routine histology but the daily workload of >recutting, adding controls and the whole IHC process is done by one person! >All of our workload is done by hand at the moment and all achieved in less >than a standard working day - usually all issued to the paths by mid >afternoon. > >>From the survey I should probably think about getting some more staff for >the IHC :-) > >Cheers > >Alan > >On 08/07/06, Rene J Buesa wrote: >> >> Jennifer: >> From a survey I finished in Feb./06 (Advance Magazine 3 July/06) I can >> give you some general information: >> 1-the average of IHC tests/year in 22 foreign labs = 18,000 >> 2-the average for USA labs (23) = 8,000 [General averages between 300 >> and 69,000 slides/year for all labs). >> 3- the difference in IHC workload between foreign and USA labs is >> significant (P<0.05) >> 4- usually the slides are cut in the same lab (average 2 hours/day). >> 5-46% of labs use autostainers (different makes). >> 6-for total workload = between 1 and 27 HTs (Average = 8; data from 122 >> labs). >> 7-lab assistants: between 0 and 7, average = 2 (48 labs). >> Hope this will help you! >> Ren? J. >> >> >> Jennifer MacDonald wrote: >> I have a few questions for IHC labs related to workload and staffing. >> Thank you. >> 1. How many slides per day? >> 2. Are slides processed, embedded, and cut by the IHC staff or elsewhere? >> 3. Automation or manual. >> If automation what instrument? >> 4. Number of staff members to perform the workload? >> How many histotechs? How many lab assistants? >> >> Thanks to all who help with this. >> Jennifer MacDonald >> Mt. San Antonio College >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> >> >> --------------------------------- >> Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great >> rates starting at 1?/min. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > >------------------------------ > >Message: 4 >Date: Sun, 9 Jul 2006 10:09:15 -0500 >From: "Orr, Rebecca" >Subject: [Histonet] RE: IHC workload >To: >Message-ID: > >Content-Type: text/plain; charset="us-ascii" > >Hi Jennifer, >Our IHC lab is separate from the Routine Histology lab. We do not rotate >techs into the IHC lab here. We have a Technical Specialist (HTL, QIHC) >who is responsible for the IHC lab. He works 7:30am-4pm. Then we have a >Tech (HT,) who comes in from 10:30am -7:00pm (she actually LIKES THAT >SHIFT!) So we have 2 people covering almost 12hours. >This helps when the Docs come in late in the afternoon with IHC >requests; we have coverage until long after they are gone to cut >everything that comes in. Overnight in the oven is a luxury we love >having. On occasion though, we do run slides the same day. > >Lately we've been looking at the LEAN 6-sigma set up where instead of >one large batch of slides once a day, we're processing several smaller >runs to cut down on total run processing time. I'd be glad to discuss >this further in a separate email, if you're interested. >We run approx. 100-150 slides/day with a Ventana Benchmark and a Biocare >Nemesis. The Autostainer platform of the Nemesis is great for working >up research projects that require larger numbers of slides. > >To finish answering your questions, the Histology lab cuts the H/E >slides. >Docs read the H/E and order IHC. We get the orders and then hunt down >the blocks (some Docs are trained to submit the blocks) and cut our own >IHC. We have a cassette holder re-alignment instrument that keeps all >of the microtomes lined up, so blocks can be cut from any microtome. >There are always cases that arise where an FNA core biopsy is submitted >and the IHC lab cuts the H/E and takes unstained slides immediately. >IHC lab does not embed. >I recommend that the person leading the IHC section have a propensity >for running these stains. It would be advantageous for this person to >have a keen interest in keeping the lab updated with new antibodies. > >It kind of depends, on your Pathologists. Our lab is part of a >teaching hospital with 13 Pathologists and a dozen Pathology residents >each doing separate research projects. We are always working on a >poster or abstract for one of them. So in our IHC lab, we need a >progressive leader who is interested in working with the consistency of >change...someone who has the experience and the progressive attitude to >research new antibodies and juggle research and clinical assignments on >a very regular basis. > >There are many labs that require a menu of 20-30 antibodies to be run >in a consistent routine, so in this case the IHC personnel requirements >might be a bit different.(My opinion) > >Hope this helps, >Becky > >Becky Orr CLA,HT(ASCP)QIHC >Assistant Manager, Anatomic Pathology >Evanston Northwestern Healthcare >847-570-2771 > > >Message: 15 >Date: Thu, 6 Jul 2006 14:43:18 -0700 >From: Jennifer MacDonald >Subject: [Histonet] IHC Workload >To: histonet@lists.utsouthwestern.edu >Message-ID: > > >Content-Type: text/plain; charset="US-ASCII" > >I have a few questions for IHC labs related to workload and staffing. >Thank you. >1. How many slides per day? >2. Are slides processed, embedded, and cut by the IHC staff or >elsewhere? >3. Automation or manual. > If automation what instrument? >4. Number of staff members to perform the workload? > How many histotechs? How many lab assistants? > >Thanks to all who help with this. >Jennifer MacDonald >Mt. San Antonio College > >------------------------------ > > > > > >------------------------------ > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >End of Histonet Digest, Vol 32, Issue 9 >*************************************** > > > >------------------------------ > >Message: 2 >Date: Mon, 10 Jul 2006 02:10:28 +0800 >From: Shirley PHUA >Subject: [Histonet] Shirley is away : 10 July 2006 (Monday) >To: histonet >Message-ID: > > >Content-Type: text/plain; charset=US-ASCII > >I will be out of the office from 10/07/2006 to 10/07/2006. > > I will return on 11 July 2006 (Tuesday). > >Pathologists : I will process your requests when I return. Otherwise, if >urgent, please contact Henry. > > > > >------------------------------ > >Message: 3 >Date: Sun, 09 Jul 2006 19:42:18 -0400 >From: "donna rossi" >Subject: [Histonet] Cryostat disinfection >To: histonet@lists.utsouthwestern.edu >Message-ID: >Content-Type: text/plain; format="flowed" > > > Here we go again! According to CAP we are to be shutting down the > cryostat and disinfecting it every week for cryostats that are used > daily. How often should it be shut down if it is used perhaps once a > week? Do you determine this by number of frozens being done or type > of tissue being cut or just pick a time frame? We are a small > hospital and this will impact our work flow but if CAP says it must be > so thennnnnnnnnnnnnn. Your help is greatly appreciated. > Donna , > Sharon Reg Health System > > >------------------------------ > >Message: 4 >Date: Mon, 10 Jul 2006 08:52:43 +0100 >From: gillian.2.brown@gsk.com >Subject: Re: [Histonet] Re: Giemsa Stain >To: RSRICHMOND@aol.com >Cc: histonet@lists.utsouthwestern.edu, > histonet-bounces@lists.utsouthwestern.edu >Message-ID: > >Content-Type: text/plain; charset=us-ascii > >Hi, >Guess no one told me how hard it is as I do make my own. We do have very >ancient stocks so maybe I wont be able to once the components have gone. > > >Regards > >Gill Brown > > > > > > >RSRICHMOND@aol.com >Sent by: histonet-bounces@lists.utsouthwestern.edu >07-Jul-2006 19:08 > >To >histonet@lists.utsouthwestern.edu >cc > >Subject >[Histonet] Re: Giemsa Stain > > > > > > >Kim Tournear asks >>I'm in need of a recipe for a giemsa stain using the >powder. I also need a recipe for making light green from powder.<< > >I've never - in more than 40 years - encountered anyone - including an >intensely giemsophilic research lab 40 years ago - Wolbach's colophonium >rosin and >all - who made up their own Giemsa stain solution from the dry powder. >It's >supposed to be extremely difficult to do. Scrupulous exclusion of water is > >essential - that applies to stock bottles of the ready-made solution also. > >Kemlo has given you adequate advice about Light Green SF. - And yes, >Kemlo, >many labs on this side of the pond have no books. American science >education >marches on into the new century. > >Bob Richmond >Knoxville TN and Gastonia NC >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > >------------------------------ > >Message: 5 >Date: Mon, 10 Jul 2006 07:36:30 -0400 >From: "Sanders, Julie, VHACIN" >Subject: [Histonet] RE: Histonet Digest, Vol 32, Issue 9 >To: >Message-ID: > <2D4ACE41DEFE93428F23D77988EFBCB50E601F@VHAV10MSGA2.v10.med.va.gov> >Content-Type: text/plain; charset="us-ascii" > > > >I have a few questions for IHC labs related to workload and staffing. >Thank you. >1. How many slides per day? >2. Are slides processed, embedded, and cut by the IHC staff or >elsewhere? >3. Automation or manual. > If automation what instrument? >4. Number of staff members to perform the workload? > How many histotechs? How many lab assistants? > >Thanks to all who help with this. >Jennifer MacDonald >Mt. San Antonio College > >Jennifer, at the VAMC Cincinnati we run between 10-30 slides per day for >IHC. The blocks are embedded and cut by the Histology staff. We have a >Ventana Benchmark which gives us the option of running overnight stains >since we only have one shift, this works great for us. There's only 3 >of us, myself and 2 techs, so we all perform IHC. >Our TAT is usually 24 hours or less, normally the docs get their IHC's >on the same day ordered if they order them before 12 noon. If it is a >longer procedure (TTF-1, etc.) we run these overnight. >Hope this helps. > >Julie > > >------------------------------ > >Message: 6 >Date: Mon, 10 Jul 2006 05:45:01 -0700 (PDT) >From: Rene J Buesa >Subject: Re: [Histonet] Cryostat disinfection >To: donna rossi , > histonet@lists.utsouthwestern.edu >Message-ID: <20060710124501.443.qmail@web61212.mail.yahoo.com> >Content-Type: text/plain; charset=iso-8859-1 > >Donna: > Regardless of the amount of frozen cases, the fundamental criteria is the type of specimen. > Do you cryosection TB cases or any which are a potential contamination source? If so you should disinfect the cryostat after each one of those cases. > CAP recommends shutting down and cleaning weekly because the probability of having those types of cases on a weekly basis. > Being a small facility I think that you should prepare a schedule based on usage of the cryostat or even better, ask CAP if they have some "latitude" regarding small facilities. > CAP is always willing to answer. > I hope this will help. > Ren? J. > >donna rossi wrote: > >Here we go again! According to CAP we are to be shutting down the >cryostat and disinfecting it every week for cryostats that are used >daily. How often should it be shut down if it is used perhaps once a >week? Do you determine this by number of frozens being done or type >of tissue being cut or just pick a time frame? We are a small >hospital and this will impact our work flow but if CAP says it must be >so thennnnnnnnnnnnnn. Your help is greatly appreciated. >Donna , >Sharon Reg Health System >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Why keep checking for Mail? The all-new Yahoo! Mail Beta shows you when there are new messages. > >------------------------------ > >Message: 7 >Date: Mon, 10 Jul 2006 08:50:14 -0400 >From: "JOHN P COLEMAN" >Subject: [Histonet] Re: Histonet Digest, Vol 32, Issue 8 >To: >Message-ID: > >Content-Type: text/plain; charset=US-ASCII > >PTAH mordant- we use bouin's and it gives good results. > >IHC staffing/workload. >I am the Senior tech for a local reference lab doing IHC for 8 >hospitals, our load is about 500 slides per day. They are cut by the IHC >staff (me and one other tech). We have 5 Biogenex i6000's, we also do >manual IF, Muscle biopsy histochemical stains and Bone Marrow >histochemical stains. Short answer- 500 slides a day, 2 techs, no >ancillary support, 5 biogenex i6000s > >John P.J. Coleman HT(ASCP)QIHC >Interim Technical Clinical Specialist >Anatomic Pathology >Sentara Laboratory Services >Cell/Voicemail(757)335-2159 >pager: (757)456-6695 > > >------------------------------ > >Message: 8 >Date: Mon, 10 Jul 2006 13:15:45 +0000 >From: "Fatma Bahar SUNAY" >Subject: [Histonet] CA IX >To: histonet@lists.utsouthwestern.edu >Message-ID: <20060710131216.M89620@balikesir.edu.tr> >Content-Type: text/plain; charset=iso-8859-9 > > >Hi everybody, > >I am looking for a reliable protocol for IHC staining of CA IX in HeLa cell >cultures. Thanks a lot. > >Dr. Bahar Sunay >Balikesir University >Turkey >-- >Open WebMail Project (http://openwebmail.org) > > > > >------------------------------ > >Message: 9 >Date: Mon, 10 Jul 2006 17:21:53 +0400 >From: "Anne Van Binsbergen" >Subject: RE: [Histonet] Cryostat disinfection >To: "Rene J Buesa" , "donna rossi" > , >Message-ID: > >Content-Type: text/plain; charset="iso-8859-1" > >Surely universal precautions apply - all specimens should be treated the same - fresh specimens are all potentially hazardous. >Common sense should prevail. > >In an HIV/TB infested country - where I come from - we had 'panic stations' if we knew the status of the patient(HIV/TB +ve), and everyone donned as much protective gear as they could find!!! - but everyone seemed to forget the first few frozens of the day, where we did not know the HIV/TB status - did that make those specimens less infectious/dangerous??? NO!!! > >If you have only one cryostat and have frequent frozens you need to take a slightly different approach - educate your surgeons and pathologists - do imprints and smears where possible - switching off, defrosting and cleaning a cryostat will cripple this sort of lab. >In my current lab I have 3 cryostats and few frozens and (lucky for me) have relatively 'safe' patients, so I can afford to defrost on a rotational basis. Not everyone is as fortunate. >Just my opinion >Annieinarabia > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa >Sent: Monday, July 10, 2006 4:45 PM >To: donna rossi; histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] Cryostat disinfection > >Donna: > Regardless of the amount of frozen cases, the fundamental criteria is the type of specimen. > Do you cryosection TB cases or any which are a potential contamination source? If so you should disinfect the cryostat after each one of those cases. > CAP recommends shutting down and cleaning weekly because the probability of having those types of cases on a weekly basis. > Being a small facility I think that you should prepare a schedule based on usage of the cryostat or even better, ask CAP if they have some "latitude" regarding small facilities. > CAP is always willing to answer. > I hope this will help. > Ren? J. > >donna rossi wrote: > >Here we go again! According to CAP we are to be shutting down the >cryostat and disinfecting it every week for cryostats that are used >daily. How often should it be shut down if it is used perhaps once a >week? Do you determine this by number of frozens being done or type >of tissue being cut or just pick a time frame? We are a small >hospital and this will impact our work flow but if CAP says it must be >so thennnnnnnnnnnnnn. Your help is greatly appreciated. >Donna , >Sharon Reg Health System >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Why keep checking for Mail? The all-new Yahoo! Mail Beta shows you when there are new messages. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >------------------------------ > >Message: 10 >Date: Mon, 10 Jul 2006 08:23:38 -0500 >From: "Dawson, Glen" >Subject: RE: [Histonet] Re: Histonet Digest, Vol 32, Issue 8 >To: "JOHN P COLEMAN" , > >Message-ID: > >Content-Type: text/plain; charset="iso-8859-1" > >John, > >All I can say is...WOW. That is an UNREAL amount of work output. I hope you are paid handsomely for this almost superhuman effort. > >Well Done, > >Glen Dawson >IHC Manager >Milwaukee, WI > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of JOHN P >COLEMAN >Sent: Monday, July 10, 2006 6:50 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Re: Histonet Digest, Vol 32, Issue 8 > > >PTAH mordant- we use bouin's and it gives good results. > >IHC staffing/workload. >I am the Senior tech for a local reference lab doing IHC for 8 >hospitals, our load is about 500 slides per day. They are cut by the IHC >staff (me and one other tech). We have 5 Biogenex i6000's, we also do >manual IF, Muscle biopsy histochemical stains and Bone Marrow >histochemical stains. Short answer- 500 slides a day, 2 techs, no >ancillary support, 5 biogenex i6000s > >John P.J. Coleman HT(ASCP)QIHC >Interim Technical Clinical Specialist >Anatomic Pathology >Sentara Laboratory Services >Cell/Voicemail(757)335-2159 >pager: (757)456-6695 >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >------------------------------ > >Message: 11 >Date: Mon, 10 Jul 2006 09:07:33 -0500 >From: "Charles Scouten" >Subject: [Histonet] RE:decontamination. >To: , "HISTONET" > >Message-ID: > <5784D843593D874C93E9BADCB87342AB01307224@tpiserver03.Coretech-holdings. com> > >Content-Type: text/plain; charset="us-ascii" > >I don't have an answer, but will post this to the histonet. You should >consider subscribing. I assume you are not presently in the market for >a self decontamininating crystat that cycle itself every night and be >ready in the morning? > > >Cordially, >Charles W. Scouten, Ph.D. >myNeuroLab.com >5918 Evergreen Blvd. >St. Louis, MO 63134 >Ph: 314 522 0300 x 342 >FAX 314 522 0377 >cwscouten@myneurolab.com >http://www.myneurolab.com > > >-----Original Message----- >From: djmr55@hotmail.com [mailto:djmr55@hotmail.com] >Sent: Saturday, July 08, 2006 1:57 PM >To: Charles Scouten >Cc: Doug Martin; Drew N. Mehta; Charles Scouten >Subject: myNeuroLab Question > >Subject:Cryostat Decontamination > >Question:I work in a small hospital histology lab. Until recently we >used chlorhexidine digluconate in 70% ETOh to decontaminate our cryostat >at -14C. CAP now says we need to disinfect with 70% and also >decontaminate with a tuberculocidal at an interval appropriate to our >facility. How do you determine what is appropriate? Number of routine >cases cut on a cryostat? We have never had anything that is suspected >of T.B., or HIV being cut on our cryostat. Is decontaminating 1 every 3 >months okay or once a year? We need to shut this thing down over a >weekend and that is another probelem with OR scheduling and no backup >instrument . HElP!! Donna Rossi (ASCP) Histosupervisor Sharon Regional >Health System > > > >------------------------------ > >Message: 12 >Date: Mon, 10 Jul 2006 09:08:26 -0600 >From: Brian Chelack >Subject: [Histonet] Re: IHC workload >To: histonet@lists.utsouthwestern.edu >Message-ID: <002a01c6a432$b23d9c20$0f13e980@PDS04> >Content-Type: text/plain; charset=us-ascii > >Some great information here, but I am wondering if you have any data >pertaining to the number of slides stained per FTE (full time equivalent) >per year. Another bit of information that would be useful is scope of >staining performed by the average lab; that is, how many different stains >are in their repertoire? The workload in a lab that runs 50,000 of the same >stain is entirely different from a lab that runs 500 slides of each of 100 >different stains. Finally, how responsible are the techs for examination of >the slides. Do they simply run the stains and ship everything out for the >pathologists, do they examine the controls only, or do they run an initial >visual screening of the cases? Each of these options changes the workload >for the techs and the pathologists dramatically. > > > >Thanks, > > > >Brian Chelack > >Prairie Diagnostic Services > >52 Campus Drive > >Saskatoon, SK > >S7N 5B4 > > > >306-966-7241 > > > > > >------------------------------ > >Message: 13 >Date: Mon, 10 Jul 2006 11:52:09 -0400 >From: "Judy Choi" >Subject: [Histonet] Troubleshoot on mounting tissue >To: histonet@lists.utsouthwestern.edu >Message-ID: >Content-Type: text/plain; format=flowed > >Hi, > >This is my first time using this listserve, so I hope this works. > >I have a question about mounting tissues (PFA perfused rat brain tissues to >be specific). I processed them for IHC using ABC complex system and DAB and >used deionized water as my mounting medium. However, when I put the tissue >in water, it swells tremendously and easily becomes wrinkled. I think as a >consequence of the swelling, there are holes throughout my tissues. (I >didn't use hydrogen peroxide until DAB, and it was at an extremely low >concentration of less than 0.1%.) > >Anyways, is my hypothesis plausible (that mounting my tissue in DI water can >cause tissues to swell and create holes)? If so, does anyone know how I can >eliminate this problem? Or can it be another factor (bad cryoprotectant, >bad perfusion, bad buffer)? I appreciate any feedback I can get. > >Thank you! >Judy > > > > > >------------------------------ > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >End of Histonet Digest, Vol 32, Issue 10 >**************************************** > From cforster <@t> umn.edu Mon Jul 10 13:36:36 2006 From: cforster <@t> umn.edu (Colleen Forster) Date: Mon Jul 10 13:36:47 2006 Subject: [Histonet] HELP... Message-ID: <44B29E34.4060601@umn.edu> Hello fellow histonetters, Please look at these 2 images and tell me what you think went wrong with the second image. they had the same fixation and processing protocol; Thanks. Colleen Forster From jhabecke <@t> fhcrc.org Mon Jul 10 14:06:26 2006 From: jhabecke <@t> fhcrc.org (Randolph-Habecker, Julie) Date: Mon Jul 10 14:06:31 2006 Subject: [Histonet] Slides for mouse rib Message-ID: <21F1955FA284134E981948D6D3FFD042053B3A2F@harpo.fhcrc.org> Folks, We are having a heck of a time keeping mouse rib on a slide during antigen retrieval and IHC. Currently we are cutting the rib on gold plus slides and baking at 60 C for 1 hour. Then the slides are air dried for several days before staining. This works fine for our human can canine bone and for mouse femur. However, the amount of bone on these mouse ribs is so thin, comes right off. Any suggestions? Thanks!! Julie Julie Randolph-Habecker, Ph.D. Staff Scientist - Manager Experimental Histopathology Shared Resource Fred Hutchinson Cancer Research Center 1100 Fairview Ave, N. M5-A803 Seattle WA 98109-1024 206-667-6119 jhabecke@fhcrc.org From PMonfils <@t> Lifespan.org Mon Jul 10 14:23:05 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Mon Jul 10 14:23:13 2006 Subject: [Histonet] Trichrome following Histofix? Message-ID: <09C945920A6B654199F7A58A1D7D1FDE01717751@lsexch.lsmaster.lifespan.org> I have received some blocks of Histofix-fixed liver, with a request for trichrome staining. The trichromes (Masson's or Gomori's) are coming out with excessive blue staining. The collagen is a nice clear blue, but everything that should be red is a dense purple. Has anyone else experienced this problem with Histofix? Any way around it? From leclercj <@t> vetanat.unizh.ch Tue Jul 11 07:09:11 2006 From: leclercj <@t> vetanat.unizh.ch (Leclerc Jocelyne) Date: Tue Jul 11 07:09:07 2006 Subject: [Histonet] Remove me please from the mail list Message-ID: It ist important that you remove me from the mailing list. I am 4 weeks in vacation. Please, please, remove me. It ist my last day work today. It ist the 4 time that I ask you to take me off. Please. Thank you. Jocelyne Leclerc VetSuisse Fakult?t Veterin?r-Anatomisches Institut Winterthurerstr. 260 8057 Z?rich From annaclaudia.esposito <@t> unina2.it Tue Jul 11 07:55:54 2006 From: annaclaudia.esposito <@t> unina2.it (annaclaudia.esposito@unina2.it) Date: Tue Jul 11 07:57:25 2006 Subject: [Histonet] biomaterials Message-ID: <1152622554.44b39fda86e77@webmail.unina2.it> Hi, I'm working with a scaffold made of polylactic acid and I' cutting frozen sections of the material embedded in tissuetek to be staine with H/O. The sections do not adhere at all on normal slides or on polylysine slides. Can anyone suggest me what kind of slide to use? thanks annaclaudia Dept of Publ, Prev and Clin Medicine Human Anatomy Unit Second University of Naples From Susan.Walzer <@t> HCAHealthcare.com Tue Jul 11 08:28:49 2006 From: Susan.Walzer <@t> HCAHealthcare.com (Walzer Susan) Date: Tue Jul 11 08:28:57 2006 Subject: [Histonet] Disposable blades In-Reply-To: <20060706163737.BKQH3326.dukecmmtao03.coxmail.com@dukecmmtao03> Message-ID: <471953BC63077941B82C26A4338272B42F049A@ORLEV03.hca.corpad.net> Accu-edge are the ONLY blades. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of crains@wpmpath.com Sent: Thursday, July 06, 2006 12:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Disposable blades In the process of changing to disposable microtome blades. Any feedback on which brands work best, or which ones to avoid? Thanks. Chris Rains WPM Pathology laboratory Salina, KS _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From n.cragg <@t> epistem.co.uk Tue Jul 11 10:01:09 2006 From: n.cragg <@t> epistem.co.uk (Nicola Cragg) Date: Tue Jul 11 10:01:17 2006 Subject: [Histonet] RE: Trichrome following Histofix? Message-ID: Hi Paul & Other Histonetters, I had the same problem with Trichrome stain on formalin fixed tissue but I found that post-fixing in Bouin's (recommended fix for Trichrome) produces beautiful staining on FFPE sections. This also worked for Van Gieson's stain, which similarly stains collagen & other connective tissues. I've just cut & paste the post-fixing part of my protocol below (I have just used the overnight at room temperature method as I was a bit scared of heating Bouin's up since it contains picric acid) if you want the full protocol let me know: - 1. Dewax in xylene 2. Rehydrate through graded alcohols to water 3. Incubate in Bouin's solution overnight at room temperature or for 1 hour at 56-60*C (sealed container in water bath, open in hood after cooling). ............proceed with stain Hope this helps!! Nicola Cragg EpiStem Ltd Manchester, UK This message has been scanned for viruses by BlackSpider MailControl - www.blackspider.com From jcline <@t> wchsys.org Tue Jul 11 11:06:27 2006 From: jcline <@t> wchsys.org (Joyce Cline) Date: Tue Jul 11 11:06:46 2006 Subject: [Histonet] RE: Mike Manzer/Formula 83, In-Reply-To: Message-ID: <000001c6a503$f830a3e0$1d2a14ac@wchsys.org> I have been using Formula 83 for about a year, but I have been using a xylene substitute for over 20 years. We keep our slides for 10 years and have no ill effects from subsititutes. -----Original Message----- From: Manzer, Mike [mailto:mmanzer@cahfs.ucdavis.edu] Sent: Friday, June 30, 2006 6:53 PM To: Joyce Cline Subject: RE: [Histonet] RE: Formula 83 Joyce, how long have you been using it? Curious about longevity of slides. Thanks, Mike ------------------------------------------------------------------------ ---- Has anyone tried this product? It is produced by CBG Biotech. Thanks and Happy 4th of July to those who ware taking a nice long weekend. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From jlinda <@t> ces.clemson.edu Tue Jul 11 12:39:41 2006 From: jlinda <@t> ces.clemson.edu (Linda Jenkins) Date: Tue Jul 11 12:37:47 2006 Subject: [Histonet] Re: biomaterials Message-ID: <5.2.1.1.2.20060711133224.01f82d20@mailhost.ces.clemson.edu> Annaclaudia, You stated: Hi, I'm working with a scaffold made of polylactic acid and I' cutting frozen sections of the material embedded in tissuetek to be staine with H/O. The sections do not adhere at all on normal slides or on polylysine slides. Can anyone suggest me what kind of slide to use? thanks annaclaudia Dept of Publ, Prev and Clin Medicine Human Anatomy Unit Second University of Naples Try formalin gelatin or Elmer's glue recipe. Polylysine does not mix well with polylactic acid and acts as a rinse agent! I will be glad to send you procedure if you can't find it in a histology text. Thanks, Linda Linda Jenkins, HT Clemson University Dept. of Bioengineering Clemson, SC 29634-0905 864.656.5553 http://www.ces.clemson.edu/bio/research/histo.htm From meligroc <@t> zgi.com Tue Jul 11 13:27:36 2006 From: meligroc <@t> zgi.com (CRME (Criss Meligro)) Date: Tue Jul 11 13:27:48 2006 Subject: [Histonet] (no subject) Message-ID: <746E68D5CBB205409B12EC32A61EE401D91B76@ned.zgi.com> We have an older Techmate 500 which is still working great but the slide racks are wearing out. Techmate accessories are no longer available or we can not find a source. Does anyone have any they are not using because of upgrading or know or a source? THANKS Criss Meligro From ROENN <@t> surgery.wisc.edu Tue Jul 11 14:56:36 2006 From: ROENN <@t> surgery.wisc.edu (Drew Allan Roenneburg) Date: Tue Jul 11 14:54:52 2006 Subject: [Histonet] P504S on FFPE mouse tissue sections Message-ID: <44B3BC240200009C00000C65@gw.surgery.wisc.edu> Has anyone had success staining mouse prostate with P504S. I'm using a rabbit anti-human polyclonal from Biocare Medical on formalin fixed, paraffin sections. HEIR with Citrate buffer and decloaking chamber. Secondary is a rabbit on rodent HRP polymer from Biocare Medical. I have tested this antibody on human breast cancer, human prostate cancer, normal mouse liver, and normal mouse kidney with intense granular staining. However, mouse prostate tumors and pin lesions remain negative. Any staining suggestions or recommendations for a different antibody? Any help would be greatly appreciated. Thanks Drew Roenneburg From cbass <@t> bidmc.harvard.edu Tue Jul 11 15:18:10 2006 From: cbass <@t> bidmc.harvard.edu (Caroline Bass) Date: Tue Jul 11 15:19:59 2006 Subject: [Histonet] affixing large tissue blocks to glass at room temperature Message-ID: <53829A45-E7C6-452E-8056-35F9088B3D72@bidmc.harvard.edu> Hey guys, I have several mouse liver "slices" that are fixed in NBF. Basically, I took a fresh liver, immersed it in NBF for about an hour, then sliced a lobe like a loaf of bread. I then put it in fix overnight to make sure the inside of the slices were well fixed. I now want to take "whole mount" pictures of the slices. It would help immensely if I could somehow fix the slices to a glass plate. The problem is that I want to make sure I don't destroy the slices as I might want to section them later. Are there any suggestions for temporarily affixing the slices to a glass plate at room temperature? Someone suggested double sided tape, but of course this could damage the tissue. Thanks, Caroline From jjenkins <@t> sch-farmville.org Tue Jul 11 15:58:04 2006 From: jjenkins <@t> sch-farmville.org (Jennie Jenkins) Date: Tue Jul 11 15:54:52 2006 Subject: [Histonet] Knife Sharpener for sale Message-ID: <44B410DC.2070406@sch-farmville.org> We have an antique for sale if anyone is interested. Its a Hacker H/I-76 Microtome Knife Sharpener and Reconditioner. We also have a blade or two to go with it. It has been well kept and has a cover. Please call 434-315-2618 with questions/offers. Jennie Jenkins Dept of Pathology 800 Oak Street Farmville, Virginia 23901 434-315-2618 434-392-8811 x 2618 ================================================== This e-mail message (and attachments) may contain information that is confidential to Southside Community Hospital. If you are not the intended recipient you cannot use, distribute or copy the message or attachments. In such a case, please notify the sender by return e-mail immediately and erase all copies of the message and attachments. Opinions, conclusions and other information in this message and attachments that do not relate to the official business of Southside Community Hospital are neither given nor endorsed by it. ================================================== From PMonfils <@t> Lifespan.org Tue Jul 11 17:11:08 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Tue Jul 11 17:11:13 2006 Subject: [Histonet] affixing large tissue blocks to glass at room temp erature Message-ID: <09C945920A6B654199F7A58A1D7D1FDE01717752@lsexch.lsmaster.lifespan.org> How about gelatin or agar or agarose? Put a drop on the glass, place the blotted tissue slice on the drop, then transfer to the refrigerator or the cold plate of the embedding unit for about a minute to gel the adhesive. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > Caroline Bass > Sent: Tuesday, July 11, 2006 1:18 PM > To: Histonet (E-mail) > Subject: [Histonet] affixing large tissue blocks to glass at room > temperature > > Hey guys, > > I have several mouse liver "slices" that are fixed in NBF. > Basically, I took a fresh liver, immersed it in NBF for about an > hour, then sliced a lobe like a loaf of bread. I then put it in fix > overnight to make sure the inside of the slices were well fixed. I > now want to take "whole mount" pictures of the slices. It would help > immensely if I could somehow fix the slices to a glass plate. The > problem is that I want to make sure I don't destroy the slices as I > might want to section them later. Are there any suggestions for > temporarily affixing the slices to a glass plate at room > temperature? Someone suggested double sided tape, but of course this > could damage the tissue. > > Thanks, > > Caroline > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From shive003 <@t> umn.edu Tue Jul 11 18:12:31 2006 From: shive003 <@t> umn.edu (Jan Shivers) Date: Tue Jul 11 18:12:37 2006 Subject: [Histonet] IHC after Wright's stain Message-ID: <022801c6a53f$7cbc5d80$a1065486@auxs.umn.edu> Hello all, I apologize if this comes across as an elementary histo question, but I'm not a histotechnologist myself, so am coming to you experts. I stain blood and cytology smears on a rare occasion with the IHC method, but have never destained and then IHC-stained the same smear. This week I attempted to do cytokeratin IHC on a nasal smear that was formerly stained with Wright's stain. It was destained with acid alcohol by the Histo lab here, and then I hand-stained it for cytokeratin using the procedure I normally do with all other smears and cytospins. The positive control CK worked fine (though admittedly it was an enzyme-digested FFPE slide and not a destained Wright's smear), but the smear (with obvious epithelial cells in it) was totally negative. Twice. Is there some constituent/step of a Wright's stain or procedure that would totally negate immunoreactivity for IHC? I assume I did not need to enzyme-digest the smear, since there was no aldehyde-type fixative used on it. Or, was the problem in the destaining reagents? The smear did go through a 100 second methanol step during the Wright's stain, but that's all the fixation it got before coming to my lab. I gave it an extra shot of 75% acetone/25% ethanol for 8 minutes, then a thorough air-drying before starting the IHC run (normally I give my smears a 10' fix in acet/etoh). Would this combination of alcohols have diminished any reactivity? Would the delay in fixing cause a 100% lack of cytokeratin staining? Thanks in advance for any input. Jan Shivers UMN Vet Diag Lab From mbmphoto <@t> gmail.com Tue Jul 11 23:25:05 2006 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Tue Jul 11 23:24:15 2006 Subject: [Histonet] antibodies/protocols for oligodendrocytes & microglia Message-ID: <71BF1A5B-3ABA-4DC1-B5D5-8FDEB71277A8@gmail.com> If there is anyone out there doing IHC/IF on primate free-floating 40um cryostat sections fixed in Zamboni fixative for Oligodendrocytes & Microglia - our lab would very much like to hear from you!!! Even though we have been successful doing IHC/IF for other markers, we have had NO success with the two above markers. We are in need of working protocol(s) & successful antibodies for Oligodendrocytes & Microglia. We have tried several antibodies for IHC/IF from several companies such as: Chemicon's anti-CNpase for oligodendrocytes - no staining. LabVision's mylein base protein (a protein that is expressed in oligodendrocytes) - it stained the mylein sheath but not the cell bodies. Biocare Medical's microglia (Iba1) antibody - also without success. I can tell you its been very frustrating trying various antibodies/ protocols - all without success on our primate sections. Please, we would greatly appreciate any information, helpful tips & equally important share your working protocol(s). We also want to hear from companies too. Yours Maria Bartola Mejia Department of Neurosurgery University California San Francisco San Francisco, Ca 94103 Tel: 415-514-2954 From wulan <@t> med.kobe-u.ac.jp Wed Jul 12 00:10:57 2006 From: wulan <@t> med.kobe-u.ac.jp (Wulan Anggrahini) Date: Wed Jul 12 00:11:04 2006 Subject: [Histonet] Alpha Smooth Muscle Actin -IHC References: <71BF1A5B-3ABA-4DC1-B5D5-8FDEB71277A8@gmail.com> Message-ID: <000a01c6a571$8eefde50$0cc8a8c0@your56ab121f6e> Is there anyone doing IHC for alpha SMA (mouse anti-aSMA Sigma, clone 1A4) on mouse cryostat section ? I did on carotid artery tissue and I used 1/100 dilution overnight and secondary antibody is Histofine Max-PO (Mouse) in 1/200 dilution overnight. Problem is keep having background with low signal/background ratio. Anyone has any tips ? I keep trying for 2 months already and now it starts to make me frustating. Thanks before Wulan Anggrahini Japan From anh2006 <@t> med.cornell.edu Wed Jul 12 00:26:15 2006 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Wed Jul 12 00:26:23 2006 Subject: [Histonet] Alpha Smooth Muscle Actin -IHC In-Reply-To: <000a01c6a571$8eefde50$0cc8a8c0@your56ab121f6e> References: <71BF1A5B-3ABA-4DC1-B5D5-8FDEB71277A8@gmail.com> <000a01c6a571$8eefde50$0cc8a8c0@your56ab121f6e> Message-ID: Use DAKO's EPOS version. It's pricey but works incredibly well with ZERO background on mouse tissue. Although I am not familiar with the secondary reagents you used it is likely that the background is from the anti-mouse secondary reacting with endogenous IgG in the tissue. If you cannot get access to EPOS you must use a mouse-on-mouse detection system. Contact me privately if you need more information. Hope this helps! Andrea >Is there anyone doing IHC for alpha SMA (mouse anti-aSMA Sigma, >clone 1A4) on mouse cryostat section ? I did on carotid artery >tissue and I used 1/100 dilution overnight and secondary antibody is >Histofine Max-PO (Mouse) in 1/200 dilution overnight. > >Problem is keep having background with low signal/background ratio. >Anyone has any tips ? I keep trying for 2 months already and now it >starts to make me frustating. Thanks before > >Wulan Anggrahini >Japan > -- From brucea <@t> unimelb.edu.au Wed Jul 12 01:27:32 2006 From: brucea <@t> unimelb.edu.au (Bruce Abaloz) Date: Wed Jul 12 01:27:55 2006 Subject: [Histonet] Disposable Blades Message-ID: FEATHER S35 get my vote........have tried/trialled ALL the others - NOTHING Compares. Cheers, Bruce in OZ -- BRUCE ABALOZ PH:61383446282 HISTOLOGIST FAX:61383447909 DEPT. of ZOOLOGY EMAIL: brucea@unimelb.edu.au THE UNIVERSITY Of MELBOURNE. VICTORIA. AUSTRALIA 3010 Nobody Can Make You Feel Inferior Without YOUR Permission - Eleanor Roosevelt DANCE LIKE NO-ONE'S WATCHING Q: What's a specimen? A: An Italian astronaut. From NSEARCY <@t> swmail.sw.org Wed Jul 12 06:39:00 2006 From: NSEARCY <@t> swmail.sw.org (Nita Searcy) Date: Wed Jul 12 06:39:17 2006 Subject: [Histonet] "Hard Copies"of Reports Message-ID: I have a question from a pathologist-"Does CAP require "hard copies" of reports?" We have an electronic medical record and are attempting to go paperless- and I am having a difficulty convincing the staff that "hard copies" sent directly to clinicians are not required. We fax to off site clients and have been following with a hard copy - which I believe is unnecessary. Thanks From JWEEMS <@t> sjha.org Wed Jul 12 07:01:12 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Jul 12 06:59:41 2006 Subject: [Histonet] "Hard Copies"of Reports Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202960375@sjhaexc02.sjha.org> We have not sent hard copies (with few exceptions) for years and years (even before I came in 1997). We fax to the clinician and all reports are imported into the electronic patient file (HPF) for Medical Records, and into the hospital system (IDX). The report is available in IDX as soon as it is signed out. Also, we now generate a frozen section report that is signed out separately from the main report (one good thing that our LIS does). It is available minutes after the frozen is completed. It is then imported into the main report. This is a new thing - much appreciated by the clinicians. Hope this might help! Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 404-851-7831 - fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Wednesday, July 12, 2006 7:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] "Hard Copies"of Reports I have a question from a pathologist-"Does CAP require "hard copies" of reports?" We have an electronic medical record and are attempting to go paperless- and I am having a difficulty convincing the staff that "hard copies" sent directly to clinicians are not required. We fax to off site clients and have been following with a hard copy - which I believe is unnecessary. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From rjbuesa <@t> yahoo.com Wed Jul 12 07:41:06 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jul 12 07:41:11 2006 Subject: [Histonet] "Hard Copies"of Reports In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD3202960375@sjhaexc02.sjha.org> Message-ID: <20060712124106.9016.qmail@web61214.mail.yahoo.com> Joyce: We used to keep hard copies of the reports in binders. They were extremely useful for the residents. Being a teaching hospital is was easy for them to go through those binders to select interesting cases to study. Having said that I think the best thing to do is to ask CAP directly about their requirement. They never complained to us about having those hard copies, as a matter of fact, they liked it, but that could be a personal preference of the CAP inspectors. CAP is always willing to clarify this type of question. Histonet will give you anecdotal information essentially "non binding" for inspection purposes. Ren? J. "Weems, Joyce" wrote: We have not sent hard copies (with few exceptions) for years and years (even before I came in 1997). We fax to the clinician and all reports are imported into the electronic patient file (HPF) for Medical Records, and into the hospital system (IDX). The report is available in IDX as soon as it is signed out. Also, we now generate a frozen section report that is signed out separately from the main report (one good thing that our LIS does). It is available minutes after the frozen is completed. It is then imported into the main report. This is a new thing - much appreciated by the clinicians. Hope this might help! Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital of Atlanta 404-851-7376 404-851-7831 - fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nita Searcy Sent: Wednesday, July 12, 2006 7:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] "Hard Copies"of Reports I have a question from a pathologist-"Does CAP require "hard copies" of reports?" We have an electronic medical record and are attempting to go paperless- and I am having a difficulty convincing the staff that "hard copies" sent directly to clinicians are not required. We fax to off site clients and have been following with a hard copy - which I believe is unnecessary. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Next-gen email? Have it all with the all-new Yahoo! Mail Beta. From mckee <@t> helix.mgh.harvard.edu Wed Jul 12 08:05:27 2006 From: mckee <@t> helix.mgh.harvard.edu (Mary McKee) Date: Wed Jul 12 08:05:33 2006 Subject: [Histonet] decalcification Message-ID: <8F2CAA6E-7092-4FF7-B83C-CEB33D511D5A@helix.mgh.harvard.edu> Hello, My lab needs to decalcify some adult mouse nasal bone for paraffin and possibly epon embedding. I'm looking for protocols. Thanks in advance. Mary PMB MGH From 2paper <@t> gmail.com Wed Jul 12 08:46:55 2006 From: 2paper <@t> gmail.com (min he) Date: Wed Jul 12 08:47:01 2006 Subject: [Histonet] How to stain mast cell in mouse lung sections with toluidine blue? Message-ID: <5cfc960b0607120646h391a7957ga6d0c60dccb7f54d@mail.gmail.com> Dear all, I am using toluidine blue to stain mast cell in mouse lung tissue sections for several times. The results are frustrating. The background of my staining is blue and the purple staining of mast cell was not observed in several sections. I use mouse ear sections as a positive control. The staining of mast cell in ear sections are much better: mast cell-purple, background blue. Would you please share your experience on mast cell staining with toluidine blue? Could it be the problem of fixation? I put the lung samples in formalin for several days before processing them. The following is the protocol I am using: ** Acidified toluidine blue *SPECIMEN PREPARATION *Cut 3 to 5 ?m thick paraffin sections from tissue fixed in 10% neutral buffered formalin. Tissue known to contain mast cells (neurofibroma, skin) is used as a control. * REAGENT PREPARATION *1 *0.5% aqueous potassium permanganate *Potassium permanganate 0.5 g Distilled water 100 ml 2 *2% aqueous potassium metabisulphite *Potassium metabisulphite 2 g Distilled water 100 ml 3 *Acidified toluidine blue solution (pH 3.2) *Distilled water 99.75 ml Glacial acetic acid 0.25 ml Toluidine Blue (CI 52040) 0.02 g * METHOD *1 Dewax and rehydrate sections. 2 Transfer sections to potassium permanganate solution for 2 minutes. 3 Rinse in distilled water. 4 Transfer sections to potassium metabisulphite solution for 1 minute (or until sections appear white). 5 Wash in tap water for 3 minutes. 6 Rinse in distilled water. 7 Place in acidified toluidine blue solution for 5 minutes. 8 Rinse in distilled water. 9 Dehydrate rapidly, clear and mount. Thank you very much. Best wishes, He Min From jamie.erickson <@t> abbott.com Wed Jul 12 08:57:25 2006 From: jamie.erickson <@t> abbott.com (Jamie E Erickson) Date: Wed Jul 12 08:57:42 2006 Subject: [Histonet] IHC on Mouse EAE brain tissue (background) Message-ID: Hi All, I'm hoping someone can help. I am staining mouse EAE brain frozen tissues with BD biosciences CD45/B220 as well as there GL7 (activated T+B cell marker) and have some background issues. The CD45 /B220 looks good with minimal background and nice cell associated staining. I had worked out these conditions on spleen for each antibody separately prior to running these brain samples. Briefly: 1. I air dried frozen brain sections overnight then fix with acetone 75%/alcohol 25% 5 minutes at RT then placed in buffer 2. DAKO block H202 0.03% 10 minutes, (CD45), 5 minutes (GL7) (WASH) 3. Streptavidin/biotin block (vector labs) 30 minutes each,(WASH) 4. serum block 10%donkey (CD45) 10% Horse (GL7) 15 minutes, (no wash) 5. CD45 or GL7 (2.5ug/ml) 1 hour RT, (wash) 6. secondary for CD45 anti-Rat (fab)2(1:500) 30 minutes RT.(wash), for GL7 anti-Rat IgM (1:200) for 30 minutes. 7. ready to use strepavidin peroxidase (vector labs ) 30 minutes RT.(wash) 8. DAB + DAKO 2-4 minutes.(wash,water) 9. Counterstain etc.. The problem is the CD45 staining has minimal background and the GL7 has much more on a serial section (could be peroxiade?). The background is a diffuse light brown staining over most of the section, Could this be due to the IgM pentameric antibody or something else I can block against? The positive control spleens had positive staining and no background...very clean in both CD45 and GL7. So I thought I was OK. Would adding 2.5% mouse serum to the block and primary help? It seems to me something about the brain samples that is causing this background. If anyone has experience background with brain IHC I'd appreciate any input you may have. Thanks _______________________________ Jamie Erickson Sr. Research Associate Department: DSMP Abbott Bioresearch Center 100 Research Drive Worcester, MA 01605-4341 508-688-3134 FAX: 508-793-4895 e-mail: jamie.erickson@abbott.com From rjbuesa <@t> yahoo.com Wed Jul 12 09:13:17 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jul 12 09:13:22 2006 Subject: [Histonet] decalcification In-Reply-To: <8F2CAA6E-7092-4FF7-B83C-CEB33D511D5A@helix.mgh.harvard.edu> Message-ID: <20060712141317.48628.qmail@web61220.mail.yahoo.com> Mary: The source of the bone is always irrelevant. Bone is bone is bone! For fast decalcification you can use HCl at 1% or any commercial decal solution (like RDO). For a gentle "friendly" (and very lengthy decalcification!) you can use EDTA. The end point of any would be your ability to either bend or press the treated bone. Any of the decalcifying methods described in any histology book can be used. The general protocol would be to place the bone and adjacent issues (AFTER being fixed) in the decal solution and inspect them every 8 hours to determine the endpoint (as explained above). Use at least a ratio of decal to bone volumes of 20 to 1 . After 24 hours, if decalcification has not taken place, eliminated decal solution and add fresh because the acid in the previous volume has been "neutralized" by the salts extracted from the bone. This is still one of the histology procedures that have to be completed "playing by ear". Hope this will help you! Ren? J. Mary McKee wrote: Hello, My lab needs to decalcify some adult mouse nasal bone for paraffin and possibly epon embedding. I'm looking for protocols. Thanks in advance. Mary PMB MGH _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail Beta. From rjbuesa <@t> yahoo.com Wed Jul 12 09:27:54 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jul 12 09:27:59 2006 Subject: [Histonet] How to stain mast cell in mouse lung sections with toluidine blue? In-Reply-To: <5cfc960b0607120646h391a7957ga6d0c60dccb7f54d@mail.gmail.com> Message-ID: <20060712142755.34357.qmail@web61217.mail.yahoo.com> He Min: Bensley & Bensley (1935) developed a method for mouse alveolar epithelium of the lung: Sections are hydrated and stained 10 minutes with 1% aq. toluidine blue solution; rinse and treat for 5 minutes with the following solution: water --- 100mL ammonium molybdate--2.5 g potassium ferrocyanide -- 0.5 g dehydrate and cover asferwards. Hope this will help you! Ren? J. min he <2paper@gmail.com> wrote: Dear all, I am using toluidine blue to stain mast cell in mouse lung tissue sections for several times. The results are frustrating. The background of my staining is blue and the purple staining of mast cell was not observed in several sections. I use mouse ear sections as a positive control. The staining of mast cell in ear sections are much better: mast cell-purple, background blue. Would you please share your experience on mast cell staining with toluidine blue? Could it be the problem of fixation? I put the lung samples in formalin for several days before processing them. The following is the protocol I am using: ** Acidified toluidine blue *SPECIMEN PREPARATION *Cut 3 to 5 ?m thick paraffin sections from tissue fixed in 10% neutral buffered formalin. Tissue known to contain mast cells (neurofibroma, skin) is used as a control. * REAGENT PREPARATION *1 *0.5% aqueous potassium permanganate *Potassium permanganate 0.5 g Distilled water 100 ml 2 *2% aqueous potassium metabisulphite *Potassium metabisulphite 2 g Distilled water 100 ml 3 *Acidified toluidine blue solution (pH 3.2) *Distilled water 99.75 ml Glacial acetic acid 0.25 ml Toluidine Blue (CI 52040) 0.02 g * METHOD *1 Dewax and rehydrate sections. 2 Transfer sections to potassium permanganate solution for 2 minutes. 3 Rinse in distilled water. 4 Transfer sections to potassium metabisulphite solution for 1 minute (or until sections appear white). 5 Wash in tap water for 3 minutes. 6 Rinse in distilled water. 7 Place in acidified toluidine blue solution for 5 minutes. 8 Rinse in distilled water. 9 Dehydrate rapidly, clear and mount. Thank you very much. Best wishes, He Min _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1¢/min. From kemlo.rogerson <@t> waht.swest.nhs.uk Wed Jul 12 09:40:10 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Jul 12 09:39:32 2006 Subject: [Histonet] How to stain mast cell in mouse lung sections with toluidine blue? Message-ID: Mast cells, as you know, contain heparin, histamine and 5 hydroxytryptamine and many people suggest that alcohol fixation may be more prudent for the preservation of the granules. Basic lead acetate fixation is recommended but some do NOT recommend formol fixation as it is thought you need a heavy metal. The presence of the sulphated polysaccharide heparin is responsible for staining with the thiazin dyes down to pH 0.5 to 1. So I assume variations in the amounts of the sulphated polysaccharide heparin may account for your problems. You may like to try other techniques such as Alcian Blue PAS, Acid diazosafranin or other stains such as Biebrich scarlet, aldehyde fuchsin, etc. I assume it's like most things in life there is no common mast cell. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 An artist is not a special kind of a person -- every person is a special kind of an artist. --Ananda Coomaraswami This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From kemlo.rogerson <@t> waht.swest.nhs.uk Wed Jul 12 09:48:08 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Jul 12 09:47:33 2006 Subject: [Histonet] decalcification Message-ID: "Bone is bone is be bone", but what you want to do after isn't always the same is it? But I agree once you know what you want to demonstrate and even more importantly, what you don't want removing (except calcium of course) there are numerous recipes you can use. More accurately "Bone is bone what else matters?" Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 An artist is not a special kind of a person -- every person is a special kind of an artist. --Ananda Coomaraswami This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From dsnider <@t> shrinenet.org Wed Jul 12 09:59:08 2006 From: dsnider <@t> shrinenet.org (Snider, Deanna) Date: Wed Jul 12 09:59:21 2006 Subject: [Histonet] Quotes for Tissue Tek IV Embedding Center Message-ID: <84BE46B37B314D409C5A17B7BAB022D6A1EA75@IDC-EX-VS01.shriners.cc> I am in need of two comparative quotes for a new Tissue Tek Iv Embedding Center. I know sales reps' watch this board and decided to give this a shot. Thanks so much! Deanna Snider HT ASCP Shriners Hospital for Children Research Dept. Cincinnatti, Oh 513-872-6388 CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients. If you are not the intended recipient, (or authorized to receive for the recipient) you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please destroy all copies of this communication and any attachments and contact the sender by reply e-mail or telephone (813) 281-0300. From Terry.Marshall <@t> rothgen.nhs.uk Wed Jul 12 10:01:07 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Wed Jul 12 10:02:09 2006 Subject: [Histonet] How to stain mast cell in mouse lung sections with toluidine blue? Message-ID: CD 117 rules, (but I far prefer looking at aldehyde fuchsin). Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:kemlo.rogerson@waht.swest.nhs.uk] Sent: 12 July 2006 15:40 To: 'min he'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] How to stain mast cell in mouse lung sections withtoluidine blue? Mast cells, as you know, contain heparin, histamine and 5 hydroxytryptamine and many people suggest that alcohol fixation may be more prudent for the preservation of the granules. Basic lead acetate fixation is recommended but some do NOT recommend formol fixation as it is thought you need a heavy metal. The presence of the sulphated polysaccharide heparin is responsible for staining with the thiazin dyes down to pH 0.5 to 1. So I assume variations in the amounts of the sulphated polysaccharide heparin may account for your problems. You may like to try other techniques such as Alcian Blue PAS, Acid diazosafranin or other stains such as Biebrich scarlet, aldehyde fuchsin, etc. I assume it's like most things in life there is no common mast cell. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 An artist is not a special kind of a person -- every person is a special kind of an artist. --Ananda Coomaraswami This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From b003046 <@t> nf.au.dk Wed Jul 12 11:41:13 2006 From: b003046 <@t> nf.au.dk (b003046@nf.au.dk) Date: Wed Jul 12 11:41:26 2006 Subject: [Histonet] mast cell staining after antigen retrieval Message-ID: <1152722473.44b52629c7786@webmail.nf.au.dk> Hi Has anyone experience with staining of mast cells with toluidine blue after immunohistochemical staining and antigen retrieval in a microwave? Mette K. Hagensen Department of cardiology Skejby hospital Denmark From weneng2004 <@t> yahoo.com Wed Jul 12 12:54:55 2006 From: weneng2004 <@t> yahoo.com (wen eng) Date: Wed Jul 12 12:54:59 2006 Subject: [Histonet] macrophage antibody Message-ID: <20060712175455.25583.qmail@web53409.mail.yahoo.com> Hello histonetters, I am looking for a macrophage antibody that works on rat paraffin sections. So far from what I searched there is only one from Cedarlane Lab. Could anybody recommend a good antibody that fits my need? Thanks in advance, Wen --------------------------------- Do you Yahoo!? Next-gen email? Have it all with the all-new Yahoo! Mail Beta. From fmoraes <@t> igc.gulbenkian.pt Wed Jul 12 13:03:02 2006 From: fmoraes <@t> igc.gulbenkian.pt (fmoraes@igc.gulbenkian.pt) Date: Wed Jul 12 13:03:10 2006 Subject: [Histonet] Alpha Smooth Muscle Actin -IHC In-Reply-To: <20060712170407.4497B6BC02A@igcns.igc.gulbenkian.pt> References: <20060712170407.4497B6BC02A@igcns.igc.gulbenkian.pt> Message-ID: <1152727382.44b539568f867@webmail.igc.gulbenkian.pt> Hi Wulan Anggrahini I am not working with cryostat sections only with Whole Mount E10.5 mouse embryos. I bought the Cy3 conjugated mouse monoclonal anti-Smooth Muscle Actin (clone 1A4 SIGMA C6198) and once is conjugated with Cy3 you don't need a 2ary Antibody. It works very well with a 1:400 dilution. Hope it helps Filipa -------------------------------- Filipa Moraes Mois?s Mallo's Lab Neural Crest Group Instituto Gulbenkian de Ci?ncia Rua da Quinta Grande 6 2800-156, Oeiras Portugal URL: www.igc.gulbenkian.pt fmoraes@igc.gulbenkian.pt Phone: +351 214464525 Message: 10 > Date: Wed, 12 Jul 2006 01:26:15 -0400 > From: "Andrea T. Hooper" > Subject: Re: [Histonet] Alpha Smooth Muscle Actin -IHC > To: Wulan Anggrahini , Histonet > > Message-ID: > Content-Type: text/plain; charset="us-ascii" ; format="flowed" > > Use DAKO's EPOS version. It's pricey but works incredibly well with > ZERO background on mouse tissue. Although I am not familiar with the > secondary reagents you used it is likely that the background is from > the anti-mouse secondary reacting with endogenous IgG in the tissue. > If you cannot get access to EPOS you must use a mouse-on-mouse > detection system. Contact me privately if you need more information. > > Hope this helps! > Andrea > > > > >Is there anyone doing IHC for alpha SMA (mouse anti-aSMA Sigma, > >clone 1A4) on mouse cryostat section ? I did on carotid artery > >tissue and I used 1/100 dilution overnight and secondary antibody is > >Histofine Max-PO (Mouse) in 1/200 dilution overnight. > > > >Problem is keep having background with low signal/background ratio. > >Anyone has any tips ? I keep trying for 2 months already and now it > >starts to make me frustating. Thanks before > > > >Wulan Anggrahini > >Japan > > > > -- > From kfineout <@t> hotmail.com Wed Jul 12 13:32:16 2006 From: kfineout <@t> hotmail.com (Kelly Larson) Date: Wed Jul 12 13:32:29 2006 Subject: [Histonet] Tissue Tek Embedding Center Quote Message-ID: I just purchased a new Tissue-Tek 5 embedding center for $9150 plus tax and delivery. From Rcartun <@t> harthosp.org Wed Jul 12 13:59:58 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Jul 12 14:00:45 2006 Subject: [Histonet] Global billing for Anatomic Pathology Message-ID: <44B50E6E0200007700000E70@hcnwgwds01.hh.chs> Do any of you work in a hospital histology lab where the pathologists bill globally (part A & part B) for pathology services? Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From liz <@t> premierlab.com Wed Jul 12 14:48:46 2006 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Wed Jul 12 14:48:56 2006 Subject: [Histonet] macrophage antibody In-Reply-To: <20060712175455.25583.qmail@web53409.mail.yahoo.com> Message-ID: <000401c6a5ec$31887850$0300a8c0@Chlipala> Wen I believe that ED-1 from serotec (it's a mouse anti-rat) will stain macrophages in rats. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of wen eng Sent: Wednesday, July 12, 2006 11:55 AM To: histonet Subject: [Histonet] macrophage antibody Hello histonetters, I am looking for a macrophage antibody that works on rat paraffin sections. So far from what I searched there is only one from Cedarlane Lab. Could anybody recommend a good antibody that fits my need? Thanks in advance, Wen --------------------------------- Do you Yahoo!? Next-gen email? Have it all with the all-new Yahoo! Mail Beta. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Wed Jul 12 15:12:03 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Jul 12 15:10:32 2006 Subject: [Histonet] Lab Services for Urologists Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD320296038A@sjhaexc02.sjha.org> Do any of you work in a hospital histology lab where urologists contract pathology services from the hospital, but they do the billing for pathology technical charges? Thank you. Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From kfineout <@t> hotmail.com Wed Jul 12 15:24:24 2006 From: kfineout <@t> hotmail.com (Kelly Larson) Date: Wed Jul 12 15:24:34 2006 Subject: [Histonet] Bone Marrow Iron Stains Message-ID: I am having a problem getting my bone marrow aspirate cell blocks and core biopsies to stain positively for Iron. The aspirate smears are staining fine. The cell block and core biopsy are fixed in 10%NBF and the core biopsy is decaled for one hour in Cal-Ex (Fisher). Any suggestions?? Kelly ([1]kfineout@hotmail.com) Pathology Services of West MI References 1. mailto:kfineout@hotmail.com From rjbuesa <@t> yahoo.com Wed Jul 12 15:37:10 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Jul 12 15:37:15 2006 Subject: [Histonet] Bone Marrow Iron Stains In-Reply-To: Message-ID: <20060712203710.21678.qmail@web61222.mail.yahoo.com> Kelly: Try decalcifying with EDTA which is a chelating agent used at pH7; not like an acid decalcifyer. It always worked fine for me. Hope this will help you! Ren? J. Kelly Larson wrote: I am having a problem getting my bone marrow aspirate cell blocks and core biopsies to stain positively for Iron. The aspirate smears are staining fine. The cell block and core biopsy are fixed in 10%NBF and the core biopsy is decaled for one hour in Cal-Ex (Fisher). Any suggestions?? Kelly ([1]kfineout@hotmail.com) Pathology Services of West MI References 1. mailto:kfineout@hotmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Next-gen email? Have it all with the all-new Yahoo! Mail Beta. From Janet.Bonner <@t> FLHOSP.ORG Wed Jul 12 15:33:38 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Wed Jul 12 15:37:19 2006 Subject: [Histonet] Bone Marrow Iron Stains References: Message-ID: Try using Immuno Rapid decal from BBC Co. Our Iron stains are beautiful on the cores now!! ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kelly Larson Sent: Wed 7/12/2006 4:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bone Marrow Iron Stains I am having a problem getting my bone marrow aspirate cell blocks and core biopsies to stain positively for Iron. The aspirate smears are staining fine. The cell block and core biopsy are fixed in 10%NBF and the core biopsy is decaled for one hour in Cal-Ex (Fisher). Any suggestions?? Kelly ([1]kfineout@hotmail.com) Pathology Services of West MI References 1. mailto:kfineout@hotmail.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Wed Jul 12 14:40:42 2006 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Jul 12 17:53:08 2006 Subject: [Histonet] capital equipment Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6C7E@EMAIL.archildrens.org> This is my lab's lucky day! We have been approved for a coverslipper and a new embedding center. Any advice out there? Your favorites? Vendors please feel welcome to contact me directly. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From histotech <@t> charter.net Wed Jul 12 19:17:50 2006 From: histotech <@t> charter.net (histotech@charter.net) Date: Wed Jul 12 19:17:56 2006 Subject: [Histonet] Bone Marrow Iron Stains Message-ID: <3405160.1152749870448.JavaMail.root@fepweb08> ---- Kelly Larson wrote: > > I am having a problem getting my bone marrow aspirate cell blocks and > core biopsies to stain positively for Iron. The aspirate smears are > staining fine. The cell block and core biopsy are fixed in 10%NBF and > the core biopsy is decaled for one hour in Cal-Ex (Fisher). Any > suggestions?? > > Kelly ([1]kfineout@hotmail.com) > > Pathology Services of West MI > > References > > 1. mailto:kfineout@hotmail.com > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet Try using B+ Fixative. If you'd like more info, contact me. From BSylinda <@t> aol.com Wed Jul 12 19:51:20 2006 From: BSylinda <@t> aol.com (BSylinda@aol.com) Date: Wed Jul 12 19:51:39 2006 Subject: [Histonet] Heavy Sheet Protectors for slides???? Message-ID: <3ad.5cc7aba.31e6f308@aol.com> Hello Histoland, Is there anyone out there familiar with this product that acts like a sheet protector, but actually holds glass slides. My pathologist is positive that they are available, but I've searched vendor to vendor with no luck. If anyone has a possible source of what I've described it would be greatly appreciated. Thanks in advance, Sylinda Battle HT ASCP From vanann702 <@t> skmc.gov.ae Thu Jul 13 01:31:09 2006 From: vanann702 <@t> skmc.gov.ae (Anne Van Binsbergen) Date: Thu Jul 13 01:29:43 2006 Subject: [Histonet] capital equipment References: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6C7E@EMAIL.archildrens.org> Message-ID: sakura!!! i have just taken delivery of an embedding centre, tape coverslipper AND an autostainer - and all are working beautifully - just like i knew they would anniinarabia ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Horn, Hazel V Sent: Wed 2006/07/12 11:40 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] capital equipment This is my lab's lucky day! We have been approved for a coverslipper and a new embedding center. Any advice out there? Your favorites? Vendors please feel welcome to contact me directly. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vanann702 <@t> skmc.gov.ae Thu Jul 13 01:34:39 2006 From: vanann702 <@t> skmc.gov.ae (Anne Van Binsbergen) Date: Thu Jul 13 01:33:44 2006 Subject: [Histonet] Heavy Sheet Protectors for slides???? References: <3ad.5cc7aba.31e6f308@aol.com> Message-ID: maybe your pathologist should give you a better discription - or tell him/her to source this 'sheet-protector-like slide holder'!! Anne ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of BSylinda@aol.com Sent: Thu 2006/07/13 04:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Heavy Sheet Protectors for slides???? Hello Histoland, Is there anyone out there familiar with this product that acts like a sheet protector, but actually holds glass slides. My pathologist is positive that they are available, but I've searched vendor to vendor with no luck. If anyone has a possible source of what I've described it would be greatly appreciated. Thanks in advance, Sylinda Battle HT ASCP _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PKamalavenkatesh <@t> wockhardtin.com Thu Jul 13 02:00:16 2006 From: PKamalavenkatesh <@t> wockhardtin.com (PKamalavenkatesh@wockhardtin.com) Date: Thu Jul 13 01:53:23 2006 Subject: [Histonet] safranin o staining-proteoglycans-thanks Message-ID: Dear Histonetters, This is with regard to safranin-O & Toluidine blue staining of rat bone cartilage. At last I got a perfect and beautiful staining of cartilage. The procedure is as given by Gayle Callis and the fixative and decalcification fluid are 10 % NBF & buffered formic acid respectively. As our experts in histonet pointed out, that the real culprit was EDTA used as decalcification fluid. Thanks to all for their sound suggestions and prompt responses. REGARDS Dr.P.Kamalavenkatesh Drug Discovery- Biology Pre clinical Safety Assessment Wockhardt Research Center, Aurangabad, India. E.mail : Pkamalavenkatesh@wockhardtin.com Please Visit our New Corporate Web Site www.wockhardt.com --------------------- Disclaimer ------------------------------------------------------------------------------ Information transmitted by this E-MAIL is proprietary to Wockhardt Ltd. and/ or its Group Companies and/or,its Customers and is intended for use only by the individual or entity to which it is addressed, and may contain information that is privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. --------------------------------------------------------------------------------------------------------------- From rjbuesa <@t> yahoo.com Thu Jul 13 06:13:30 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jul 13 06:13:34 2006 Subject: [Histonet] capital equipment In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6C7E@EMAIL.archildrens.org> Message-ID: <20060713111330.77964.qmail@web61223.mail.yahoo.com> Remember that you get what you pay for! The best, even if in the high price range: Sakura Finetek. You will not be sorry! Extremely dependable and good technical support. Ren? J. "Horn, Hazel V" wrote: This is my lab's lucky day! We have been approved for a coverslipper and a new embedding center. Any advice out there? Your favorites? Vendors please feel welcome to contact me directly. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Sneak preview the all-new Yahoo.com. It's not radically different. Just radically better. From rjbuesa <@t> yahoo.com Thu Jul 13 06:17:25 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jul 13 06:17:28 2006 Subject: [Histonet] Heavy Sheet Protectors for slides???? In-Reply-To: <3ad.5cc7aba.31e6f308@aol.com> Message-ID: <20060713111725.66076.qmail@web61224.mail.yahoo.com> I am quite sure that your PT is referring to a "slides folder" that can accommodate 20 slides in 2 rows. Any supplies catalog should have it. Ren? J. BSylinda@aol.com wrote: Hello Histoland, Is there anyone out there familiar with this product that acts like a sheet protector, but actually holds glass slides. My pathologist is positive that they are available, but I've searched vendor to vendor with no luck. If anyone has a possible source of what I've described it would be greatly appreciated. Thanks in advance, Sylinda Battle HT ASCP _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. From trine.gejsing <@t> leo-pharma.com Thu Jul 13 06:24:01 2006 From: trine.gejsing <@t> leo-pharma.com (trine.gejsing@leo-pharma.com) Date: Thu Jul 13 06:24:12 2006 Subject: [Histonet] (no subject) Message-ID: I'm looking for the "slides-folder" too. We had some containing special staining control slides at my old work, but they dont remember where they got it. Please give a webpage or a phonenumber Trine LEO-Pharma, Denmark This e-mail, inclusive of attachments, is intended for the person(s) stated above and may contain confidential information. Unauthorised reading, disclosure, copying, distribution or use of this information may violate rights to proprietary information. If you are not an intended recipient, please return this e-mail to the sender and delete your copy. Thank you. From trine.gejsing <@t> leo-pharma.com Thu Jul 13 06:39:31 2006 From: trine.gejsing <@t> leo-pharma.com (trine.gejsing@leo-pharma.com) Date: Thu Jul 13 06:39:42 2006 Subject: [Histonet] Slide folder Message-ID: I found the slide folders !!!!!!!!!!!!!!!!!! www.emsdiasum.com /microscopy/products/preparation/slide_storage This e-mail, inclusive of attachments, is intended for the person(s) stated above and may contain confidential information. Unauthorised reading, disclosure, copying, distribution or use of this information may violate rights to proprietary information. If you are not an intended recipient, please return this e-mail to the sender and delete your copy. Thank you. From katherine-walters <@t> uiowa.edu Thu Jul 13 08:14:40 2006 From: katherine-walters <@t> uiowa.edu (Walters, Katherine S) Date: Thu Jul 13 08:14:46 2006 Subject: [Histonet] Alizarin red color differences Message-ID: Esteemed Histonetters, I have two powders of alizarin red from different sources that are two very distinctly different colors. I am not sure which would be the best to use. One is very dark red and the other is a deep yellow. Are these both useable alizarin red powders? There aren't any CI numbers on either one. Can they be used at different pHs? Please help me sort this out. Thanks, Kathy From JWEEMS <@t> sjha.org Thu Jul 13 08:36:28 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Thu Jul 13 08:34:53 2006 Subject: [Histonet] Heavy Sheet Protectors for slides???? Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32029603B0@sjhaexc02.sjha.org> I have seen those and VWR comes to mind. I don't have time to search, but you might start there. I would search for slide protectors to see what happens..good luck! j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of BSylinda@aol.com Sent: Wednesday, July 12, 2006 8:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Heavy Sheet Protectors for slides???? Hello Histoland, Is there anyone out there familiar with this product that acts like a sheet protector, but actually holds glass slides. My pathologist is positive that they are available, but I've searched vendor to vendor with no luck. If anyone has a possible source of what I've described it would be greatly appreciated. Thanks in advance, Sylinda Battle HT ASCP _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From kgrobert <@t> rci.rutgers.edu Thu Jul 13 08:41:37 2006 From: kgrobert <@t> rci.rutgers.edu (Kathleen Roberts) Date: Thu Jul 13 08:40:22 2006 Subject: [Histonet] Heavy Sheet Protectors for slides???? In-Reply-To: <3ad.5cc7aba.31e6f308@aol.com> References: <3ad.5cc7aba.31e6f308@aol.com> Message-ID: <44B64D91.2010609@rci.rutgers.edu> Is this it? This is from Electron Microscopy Sciences. View-Pack ? Microscope Slide Holder With 3-Ring Binder For the orderly visual display and safe transport of microscope slides. This loose-leaf binder has ten vinyl View-Pack? pages (hold 160 slides). Each page holds 16 slides 3x1" (75x25mm) in individual pockets with a white back and clear front. A flap in the center keeps slides from falling out. Simply fold the page at the center crease to retrieve slides. The 8?" x 10?" (22x27cm) View-Pack pages comes with a standard 9"x12" (23x30cm) 3-ring binder. 71544-01 View-Pack With 3-Ring Binder each 52.00 cart 71544-50 Refill View-Pack 10/pk 34.00 cart BSylinda@aol.com wrote: >Hello Histoland, > >Is there anyone out there familiar with this product that acts like a sheet >protector, but actually holds glass slides. My pathologist is positive that >they are available, but I've searched vendor to vendor with no luck. If >anyone has a possible source of what I've described it would be greatly >appreciated. > >Thanks in advance, >Sylinda Battle HT ASCP >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From kemlo.rogerson <@t> waht.swest.nhs.uk Thu Jul 13 08:48:38 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Jul 13 08:48:00 2006 Subject: [Histonet] Alizarin red color differences Message-ID: The Alizarins posses one or more o-diphenol groups which serves as a metal chelation site. Alizarin Red S forms scarlet lakes with aluminium and barium and a crimson lake with calcium. Magnesium gives a clear scarlet solution whilst mercury a clear dark red solution. Alizarin (CI 58000) stains calcium selectively around pH 12 and Alizarin Red S (CI 580005) at pH 9. Alizarin Red S is the monosulphanate of Alizarin and what I'm trying to say is that there are many alizarins. You may have Alizarin or Alizarin Red S; I am confused why it is yellow. They work differently and at different pHs; it is dangerous to use something you aren't sure of. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 I try to avoid looking forward or backward, and try to keep looking upward. --Charlotte Bronte This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From tkngflght <@t> yahoo.com Thu Jul 13 09:48:00 2006 From: tkngflght <@t> yahoo.com (Cheryl) Date: Thu Jul 13 09:48:06 2006 Subject: [Histonet] open position in SC--any histotechs wanting to learn gross? Message-ID: <20060713144800.74124.qmail@web50914.mail.yahoo.com> Hi All! I'm working with a lab in South Carolina and they need a tech. I've worked with them in the past and they are a good group, treat their techs well and are growing. They need help! If you're a histotech (registered or not) and think you might meet the CLIA 88 requirements for grossing (if you aren't sure but are interested call me!) and would like a job in a great location, working with good people and a chance to grow with a private lab-- Please contact me privately at the below email/phone. Cheryl Kerry, HT(ASCP) Full Staff Inc 800.756.3309 phone and fax admin@fullstaff.org From h-tang2 <@t> northwestern.edu Thu Jul 13 09:57:19 2006 From: h-tang2 <@t> northwestern.edu (h-tang2@northwestern.edu) Date: Thu Jul 13 09:57:26 2006 Subject: [Histonet] help with Technovit 8100 embedding/section Message-ID: <20060713145720.045C6BF@lulu.it.northwestern.edu> Dear members, I am trying to view in vivo transfected GFP expression in mouse testis so used glycol methacylate(Technovit 8100) to embed the testis after fixation, acetone dehydration and infilitration. The polymerized tissue-block was too hard and crisp to cut, the microtome experienced extreme shattering. I was only able to get shatters instead of tissue section. Any comments and suggestion is greatly appreciated! Huanghui Tang Northwestern University From MSHERWOOD <@t> PARTNERS.ORG Thu Jul 13 09:58:07 2006 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Thu Jul 13 09:58:15 2006 Subject: [Histonet] Hooves, etc. Message-ID: <1AF23D0AD12E7444A5DB083CA978B73407A30A4C@PHSXMB1.partners.org> Our histotech is having a difficult time keeping sections of pig hooves and nails on her slides. She uses the superfrost plus microscope slides. Any suggestions would be appreciated. Also, if anybody has any protocols for decalcification of rat knees, please respond. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) msherwood@partners.org From la.sebree <@t> hosp.wisc.edu Thu Jul 13 10:07:47 2006 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Thu Jul 13 10:20:40 2006 Subject: [Histonet] ST14 Message-ID: Hello, Has anyone had experience with mouse polyclonal antibody ST14 from Abnova? Thanks, Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From twebster <@t> nmcinc.org Thu Jul 13 10:30:01 2006 From: twebster <@t> nmcinc.org (Tim Webster) Date: Thu Jul 13 10:30:09 2006 Subject: [Histonet] Unsubscribe In-Reply-To: <20060709165249.B438FA7B5F@zixvpm01.nmcinc.org> Message-ID: <0CBF5C420BD8AD4AAEDED774BD4A240F17BA22@exchange.nmcinc.org> Tim Webster Histology Specialist Northwestern Medical Center 133 Fairfield Street St Albans, VT 05478 (802) 524-1070 x4349 (Dept. & Voice mail) twebster@nmcinc.org Statement of Confidentiality This message and any attachments are from NMC and intended only for the addressee(s). The information contained may include privileged or other confidential information. Unauthorized review, forwarding, printing, copying or distribution is strictly prohibited. Any opinions expressed are those of the author and not necessarily those of Norhtwestern Medical Center. If you should receive this message in error, please delete it and notify the sender. Thank you. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Sunday, July 09, 2006 12:53 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 32, Issue 9 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: Mordant for PTAH (RSRICHMOND@aol.com) 2. Special stains and IHC for Gastric Cancer (Diana Schleicher) 3. Re: IHC Workload (Alan Bishop) 4. RE: IHC workload (Orr, Rebecca) ---------------------------------------------------------------------- Message: 1 Date: Sat, 8 Jul 2006 14:10:50 EDT From: RSRICHMOND@aol.com Subject: [Histonet] Re: Mordant for PTAH To: histonet@lists.utsouthwestern.edu Message-ID: <431.473e7b6.31e14f2a@aol.com> Content-Type: text/plain; charset="ISO-8859-1" Charlene Henry HT (ASCP), QIHC at St. Jude Children's Research Hospital [Memphis, Tennessee] asks: >>We are trying to rid the lab of all mercury products and I was wondering what everyone is using as a mordant for the PTAH. We are currently using 4% mercuric chloride and I would like to replace it with another mordant.<< and Rene J Buesa replies>>Try using 5% potassium dichromate at 60?C for 30 minutes. It has worked for me.<< But then you have to dispose of chromium, which is as difficult as mercury to get hauled away, I think. But what does anyone use phosphotungstic acid hematoxylin (PTAH) for nowadays anyway? Forty years ago it was of some use for staining muscle striations (in suspected rhabdomyosarcomas) and astrocytes - if the tissue had been fixed in Zenker/Helly (mercury, chromium, and formaldehyde). I recall it being said that the chief virtue of PTAH was that it took overnight to do the stain, and that gave you time enough to try to identify an unusual tumor. (It also took three months to make PTAH - you didn't add an oxidant, just put it on the back porch like sun tea.) According to Giuseppe Verdi or his librettist, Aida praised it to the skies ("Omnipotente Ptah" - sorry about that), but I think PTAH's fortunes have been in decline ever since. Bob Richmond Knoxville TN and Gastonia NC ------------------------------ Message: 2 Date: Sat, 8 Jul 2006 12:30:14 -0700 (PDT) From: Diana Schleicher Subject: [Histonet] Special stains and IHC for Gastric Cancer To: histonet@lists.utsouthwestern.edu Message-ID: <20060708193014.94230.qmail@web53503.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I am currently a histotech student. I am writing a paper and preparing a power point presentation on the subject of Gastric Cancer. I would like to find out what tests labs are performing for this disease. Thank you. Diana --------------------------------- Sneak preview the all-new Yahoo.com. It's not radically different. Just radically better. ------------------------------ Message: 3 Date: Sun, 9 Jul 2006 11:43:31 +1200 From: "Alan Bishop" Subject: Re: [Histonet] IHC Workload To: "Rene J Buesa" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1; format=flowed Some interesting stats there. Our lab does around 20,000 IHC slides a year. Most blocks already processed and embedded from us doing the routine histology but the daily workload of recutting, adding controls and the whole IHC process is done by one person! All of our workload is done by hand at the moment and all achieved in less than a standard working day - usually all issued to the paths by mid afternoon. >From the survey I should probably think about getting some more staff for the IHC :-) Cheers Alan On 08/07/06, Rene J Buesa wrote: > > Jennifer: > From a survey I finished in Feb./06 (Advance Magazine 3 July/06) I can > give you some general information: > 1-the average of IHC tests/year in 22 foreign labs = 18,000 > 2-the average for USA labs (23) = 8,000 [General averages between 300 > and 69,000 slides/year for all labs). > 3- the difference in IHC workload between foreign and USA labs is > significant (P<0.05) > 4- usually the slides are cut in the same lab (average 2 hours/day). > 5-46% of labs use autostainers (different makes). > 6-for total workload = between 1 and 27 HTs (Average = 8; data from 122 > labs). > 7-lab assistants: between 0 and 7, average = 2 (48 labs). > Hope this will help you! > Ren? J. > > > Jennifer MacDonald wrote: > I have a few questions for IHC labs related to workload and staffing. > Thank you. > 1. How many slides per day? > 2. Are slides processed, embedded, and cut by the IHC staff or elsewhere? > 3. Automation or manual. > If automation what instrument? > 4. Number of staff members to perform the workload? > How many histotechs? How many lab assistants? > > Thanks to all who help with this. > Jennifer MacDonald > Mt. San Antonio College > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great > rates starting at 1?/min. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 4 Date: Sun, 9 Jul 2006 10:09:15 -0500 From: "Orr, Rebecca" Subject: [Histonet] RE: IHC workload To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi Jennifer, Our IHC lab is separate from the Routine Histology lab. We do not rotate techs into the IHC lab here. We have a Technical Specialist (HTL, QIHC) who is responsible for the IHC lab. He works 7:30am-4pm. Then we have a Tech (HT,) who comes in from 10:30am -7:00pm (she actually LIKES THAT SHIFT!) So we have 2 people covering almost 12hours. This helps when the Docs come in late in the afternoon with IHC requests; we have coverage until long after they are gone to cut everything that comes in. Overnight in the oven is a luxury we love having. On occasion though, we do run slides the same day. Lately we've been looking at the LEAN 6-sigma set up where instead of one large batch of slides once a day, we're processing several smaller runs to cut down on total run processing time. I'd be glad to discuss this further in a separate email, if you're interested. We run approx. 100-150 slides/day with a Ventana Benchmark and a Biocare Nemesis. The Autostainer platform of the Nemesis is great for working up research projects that require larger numbers of slides. To finish answering your questions, the Histology lab cuts the H/E slides. Docs read the H/E and order IHC. We get the orders and then hunt down the blocks (some Docs are trained to submit the blocks) and cut our own IHC. We have a cassette holder re-alignment instrument that keeps all of the microtomes lined up, so blocks can be cut from any microtome. There are always cases that arise where an FNA core biopsy is submitted and the IHC lab cuts the H/E and takes unstained slides immediately. IHC lab does not embed. I recommend that the person leading the IHC section have a propensity for running these stains. It would be advantageous for this person to have a keen interest in keeping the lab updated with new antibodies. It kind of depends, on your Pathologists. Our lab is part of a teaching hospital with 13 Pathologists and a dozen Pathology residents each doing separate research projects. We are always working on a poster or abstract for one of them. So in our IHC lab, we need a progressive leader who is interested in working with the consistency of change...someone who has the experience and the progressive attitude to research new antibodies and juggle research and clinical assignments on a very regular basis. There are many labs that require a menu of 20-30 antibodies to be run in a consistent routine, so in this case the IHC personnel requirements might be a bit different.(My opinion) Hope this helps, Becky Becky Orr CLA,HT(ASCP)QIHC Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2771 Message: 15 Date: Thu, 6 Jul 2006 14:43:18 -0700 From: Jennifer MacDonald Subject: [Histonet] IHC Workload To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" I have a few questions for IHC labs related to workload and staffing. Thank you. 1. How many slides per day? 2. Are slides processed, embedded, and cut by the IHC staff or elsewhere? 3. Automation or manual. If automation what instrument? 4. Number of staff members to perform the workload? How many histotechs? How many lab assistants? Thanks to all who help with this. Jennifer MacDonald Mt. San Antonio College ------------------------------ ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 32, Issue 9 *************************************** From DEllenburg2 <@t> stfrancishealth.org Thu Jul 13 10:48:07 2006 From: DEllenburg2 <@t> stfrancishealth.org (DEllenburg2@stfrancishealth.org) Date: Thu Jul 13 10:47:29 2006 Subject: [Histonet] CAP STANDARDS ANP. 12087 Message-ID: <0A502E8156DAA4468CB8979B27177555016CC7AE@BSSMSX5501> Greetings to all Histonetters, For CAP question ANP 12087 in regards to decontamination of the cryostat. The note states that decontamination of the interior of cryostats may be accomplished with 70% ethanol and in addition to this decontamination process, the cryostat should be defrosted and decontaminated with a tuberculocidal disinfectant at an interval appropriate for the institution. I was wondering if other institutions would mind sharing what they use for their tuberculocidal disinfectant and where to purchase it from. Thanks for any help or suggestions. Debbie Ellenburg The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone at (864) 255-1000, and permanently delete the original e-mail, attachment(s), and any copies. From RCazares <@t> schosp.org Thu Jul 13 10:55:27 2006 From: RCazares <@t> schosp.org (Cazares, Ruth) Date: Thu Jul 13 10:55:32 2006 Subject: [Histonet] Traveling histotech Message-ID: <913FAC2B773C19488E26AE6572180FA505B27EEA@exch01.schosp.org> Hi all, Recently, about a month or two ago someone posted on the histonet that they were looking for histotechs to do temp jobs. This person encouraged anyone interested to respond and even those who just wanted to know how the traveling tech thing worked. I have deleted the post instead of saving it. So if this person can contact me I would sure appreciate it. Thank you Ruth Cazares *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From pwg1 <@t> cdc.gov Thu Jul 13 11:16:58 2006 From: pwg1 <@t> cdc.gov (Greer, Patricia (CDC/NCID/VR)) Date: Thu Jul 13 11:34:41 2006 Subject: [Histonet] Heavy Sheet Protectors for slides???? Message-ID: Sylinda, Our pathologists have used the following sheet protector: http://service.belart.com/cat/441700000.html Hope this is what you are looking for. Pat Greer Infectious Disease Pathology Activity Centers for Disease Control and Prevention Mail Stop G-32 Atlanta, GA 30333 404-639-2811 Is there anyone out there familiar with this product that acts like a sheet protector, but actually holds glass slides. My pathologist is positive that they are available, but I've searched vendor to vendor with no luck. If anyone has a possible source of what I've described it would be greatly appreciated. Thanks in advance, Sylinda Battle HT ASCP From kbradshaw <@t> lcpath.com Thu Jul 13 11:41:33 2006 From: kbradshaw <@t> lcpath.com (Kari Bradshaw) Date: Thu Jul 13 11:41:47 2006 Subject: [Histonet] CAP STANDARDS ANP. 12087 In-Reply-To: <0A502E8156DAA4468CB8979B27177555016CC7AE@BSSMSX5501> Message-ID: <003401c6a69b$33af8380$9401a8c0@LCPATH.COM> We buy Viraguard wipes and spray to decontaminate our cryostat. It is a virucide, tuberculocide, bactericide, and fungicide. We have been using it for about 3 months. The greatest thing about the product is, it's alcohols based so it doesn't freeze. You can decontaminate without turning off your machine. Veridien 1-800-345-5444 www.veridien.com Product number 10160 wipes 11016 bottled spray 11128 gallon Kari L. Bradshaw Laboratory Manager Lower Columbia Pathologists 1217 14th Ave Longview, WA 98632 360-425-5620 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of DEllenburg2@stfrancishealth.org Sent: Thursday, July 13, 2006 8:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP STANDARDS ANP. 12087 Greetings to all Histonetters, For CAP question ANP 12087 in regards to decontamination of the cryostat. The note states that decontamination of the interior of cryostats may be accomplished with 70% ethanol and in addition to this decontamination process, the cryostat should be defrosted and decontaminated with a tuberculocidal disinfectant at an interval appropriate for the institution. I was wondering if other institutions would mind sharing what they use for their tuberculocidal disinfectant and where to purchase it from. Thanks for any help or suggestions. Debbie Ellenburg The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone at (864) 255-1000, and permanently delete the original e-mail, attachment(s), and any copies. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From melissa.mazan <@t> tufts.edu Thu Jul 13 12:11:58 2006 From: melissa.mazan <@t> tufts.edu (Melissa Mazan) Date: Thu Jul 13 12:12:04 2006 Subject: [Histonet] tissue loss Message-ID: <44B67EDE.2090801@tufts.edu> Hi - I'm trying a citrate buffer antigen retrieval protocol. After I have dewaxed the slides, I put them in citrate buffer - protocol says microwave on high for 25 minutes. However, when I remove the slides all the tissue has slid off the slide. Any suggestions? Melissa -- Melissa R. Mazan, DVM, Diplomate ACVIM Associate Professor of Large Animal Medicine Director of Sports Medicine Cummings School of Veterinary Medicine Tufts University 200 Westborough Road North Grafton, MA 01536 Tel: 508-839-5395 Fax: 508-839-7922 Email: melissa.mazan@tufts.edu From tanjie <@t> u.washington.edu Thu Jul 13 12:55:44 2006 From: tanjie <@t> u.washington.edu (Elaine Tanji) Date: Thu Jul 13 12:55:51 2006 Subject: [Histonet] Histo Tech Full Time Seattle Message-ID: <44B68920.4020407@u.washington.edu> The University of Washington Medical Center in Seattle currently has an opening for a full time Histo Tech. If interested, please link to the UWMC Staff Jobs page below and enter position number 23959. Thank you. http://www.washington.edu/admin/hr/jobs/apl/ From kfineout <@t> hotmail.com Thu Jul 13 13:02:11 2006 From: kfineout <@t> hotmail.com (Kelly Larson) Date: Thu Jul 13 13:02:16 2006 Subject: [Histonet] Coverslipper/Embedding Center Message-ID: I always buy Sakura (Tissue-Tek). I just bought a Tissue-Tek 5 embedding center and love it!! I don't have an automated coverslipper where I am at now but I did use the Sakura coverslipper that used a film instead of glass. I really liked that too. From vazquezr <@t> ohsu.edu Thu Jul 13 13:34:00 2006 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Thu Jul 13 13:34:33 2006 Subject: [Histonet] CAP STANDARDS ANP. 12087 Message-ID: I use Sanimaster 4. It is a broadspectrum disinfectant and non-corrosive. Robyn OHSU From rjbuesa <@t> yahoo.com Thu Jul 13 13:40:01 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jul 13 13:40:11 2006 Subject: [Histonet] Hooves, etc. In-Reply-To: <1AF23D0AD12E7444A5DB083CA978B73407A30A4C@PHSXMB1.partners.org> Message-ID: <20060713184001.24176.qmail@web61221.mail.yahoo.com> Peggy: Under separate cover I am forwarding a general protocol for Toenails that I am sure will be useful for you. Regards Ren? J. "Sherwood, Margaret " wrote: Our histotech is having a difficult time keeping sections of pig hooves and nails on her slides. She uses the superfrost plus microscope slides. Any suggestions would be appreciated. Also, if anybody has any protocols for decalcification of rat knees, please respond. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) msherwood@partners.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. From rjbuesa <@t> yahoo.com Thu Jul 13 13:49:52 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jul 13 13:50:00 2006 Subject: [Histonet] tissue loss In-Reply-To: <44B67EDE.2090801@tufts.edu> Message-ID: <20060713184952.51048.qmail@web61223.mail.yahoo.com> Melissa: This is the way I used to do HIER: heat the citrate buffer in the MWoven without the slides up to boliling point (time will depend on the energy output of yout MWoven and the amount of buffer). After that, put the heated citrate buffer in either a water bath (95?C) or a steamer (98?C) and when the hot citrate has thermally equilibrated with the hot environment into which it has been put (about 5 minutes) is then when you add the slides to do the HIER during 20 minutes; after those 20 minutes you take the container with the hot citrate buffer and the slides and place it over the counter for another 20 minutes to complete HIER. You will not lose sections with this procedure. Hope this will help you Ren? J. Melissa Mazan wrote: Hi - I'm trying a citrate buffer antigen retrieval protocol. After I have dewaxed the slides, I put them in citrate buffer - protocol says microwave on high for 25 minutes. However, when I remove the slides all the tissue has slid off the slide. Any suggestions? Melissa -- Melissa R. Mazan, DVM, Diplomate ACVIM Associate Professor of Large Animal Medicine Director of Sports Medicine Cummings School of Veterinary Medicine Tufts University 200 Westborough Road North Grafton, MA 01536 Tel: 508-839-5395 Fax: 508-839-7922 Email: melissa.mazan@tufts.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Sneak preview the all-new Yahoo.com. It's not radically different. Just radically better. From kweidenh <@t> montefiore.org Thu Jul 13 14:03:37 2006 From: kweidenh <@t> montefiore.org (Karen Weidenheim) Date: Thu Jul 13 14:04:14 2006 Subject: [Histonet] CAP STANDARDS ANP. 12087 Message-ID: Where do you purchase Sanimaster 4? Karen M. Weidenheim, M.D. Professor of Pathology, Clinical Neurology and Clinical Neurosurgery Albert Einstein College of Medicine Chief, Division of Neuropathology Montefiore Medical Center 111 East 210th Street Bronx, NY 10467 (718) 920-4446 FAX (718) 653-3409 Beeper (917) 556 3696 CONFIDENTIALITY NOTICE: This email, including any attachments, is for the sole use of the intended recipient(s). The information contained in this message may be private and confidential, and may also be subject to the work product doctrine. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. Thank you. >>> "Robyn Vazquez" 07/13/06 2:34 PM >>> I use Sanimaster 4. It is a broadspectrum disinfectant and non-corrosive. Robyn OHSU _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LRaff <@t> lab.uropartners.com Thu Jul 13 14:57:39 2006 From: LRaff <@t> lab.uropartners.com (Lester Raff) Date: Thu Jul 13 14:57:45 2006 Subject: [Histonet] Non-histology question Message-ID: <5DA1CA5D0B98A84985B545A24423B822019896@UPLAB01.uplab.local> I have noticed some biochemists and lab directors on this net, so here is a question for you. Do any off you know of an instrument that does FREE Testosterone? Thanks, Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 From vazquezr <@t> ohsu.edu Thu Jul 13 14:59:59 2006 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Thu Jul 13 15:00:27 2006 Subject: [Histonet] CAP STANDARDS ANP. 12087 Message-ID: Our housekeeping obtains it. I have Googled it and seen it. Just type in the name and you will find the website. Robyn OHSU 503-494-4658 From histotech <@t> charter.net Thu Jul 13 15:37:09 2006 From: histotech <@t> charter.net (histotech@charter.net) Date: Thu Jul 13 15:37:14 2006 Subject: [Histonet] Microwave Tissue Processors Message-ID: <484060284.1152823030006.JavaMail.root@fepweb12> Hello, I'd like some opinions on microwave tissue processors. How well do they work with breast, colon and GI specimens? Thanks, Dianne D From PMonfils <@t> Lifespan.org Thu Jul 13 18:05:37 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Jul 13 18:05:42 2006 Subject: [Histonet] magnetized tissue?? Message-ID: <09C945920A6B654199F7A58A1D7D1FDE01717755@lsexch.lsmaster.lifespan.org> I have noticed an odd phenomenon many times, so I assume others must have noticed it too? I often have slender, cylindrical specimens like vessels or mouse spinal cord that have to be cut in cross section and therefore have to be embedded "on end". Sometimes a specimen is a little too long to mount on end without the cassette resting on top of the specimen. In such cases I take a scalpel blade and trim a bit off the length, to make the specimen "shorter". When I take a specimen that has been trimmed in this way, and drop it back into the stainless steel embedding mold filled with paraffin, the specimen instantly flips around and attaches on end to the bottom of the mold, always by the freshly cut end, just as though it somehow became "magnetized" by my trimming it. Has anyone else observed this? (Please say yes, I can't be the only one!) If so, does anyone have any idea how/why this occurs? Obviously it is not true magnetism operating here because (1) tissue does not become magnetized, and (2) stainless steel is not attracted to a magnet. From mtarango <@t> nvcancer.org Thu Jul 13 19:16:55 2006 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Thu Jul 13 19:17:21 2006 Subject: [Histonet] Follicular Lymphoma Message-ID: <5AEC610C1CE02945BD63A395BA763EDE82E514@NVCIEXCH02.NVCI.org> Does anyone know whether a patient who's treated with Rituximab and has a multiple paratrabecular lymphoid aggregates which stain CD20 negative, should also stain CD79A negative? I can understand the CD20 being negative but me and the docs aren't sure about CD79a also being negative. Flow cytometry also got nothing positive for CD20. Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 "MMS " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From trine.gejsing <@t> leo-pharma.com Fri Jul 14 00:09:02 2006 From: trine.gejsing <@t> leo-pharma.com (trine.gejsing@leo-pharma.com) Date: Fri Jul 14 00:09:53 2006 Subject: Fw: [Histonet] Heavy Sheet Protectors for slides???? Message-ID: ----- Forwarded by Trine Gejsing/Ballerup/DK/LEO on 14-07-2006 07:06 ----- Thats the one. Found out yesterday. Superb................ :0) To BSylinda@aol.com cc "'histonet@lists.utsouthwestern.edu'" Subject Re: [Histonet] Heavy Sheet Protectors for slides???? Is this it? This is from Electron Microscopy Sciences. View-Pack ? Microscope Slide Holder With 3-Ring Binder For the orderly visual display and safe transport of microscope slides. This loose-leaf binder has ten vinyl View-Pack? pages (hold 160 slides). Each page holds 16 slides 3x1" (75x25mm) in individual pockets with a white back and clear front. A flap in the center keeps slides from falling out. Simply fold the page at the center crease to retrieve slides. The 8?" x 10?" (22x27cm) View-Pack pages comes with a standard 9"x12" (23x30cm) 3-ring binder. 71544-01 View-Pack With 3-Ring Binder each 52.00 cart < http://www.emsdiasum.com/microscopy/cartmxc/addtocart.asp?UCII_AddId=71544-01 > 71544-50 Refill View-Pack 10/pk 34.00 cart < http://www.emsdiasum.com/microscopy/cartmxc/addtocart.asp?UCII_AddId=71544-50 > BSylinda@aol.com wrote: >Hello Histoland, > >Is there anyone out there familiar with this product that acts like a sheet >protector, but actually holds glass slides. My pathologist is positive that >they are available, but I've searched vendor to vendor with no luck. If >anyone has a possible source of what I've described it would be greatly >appreciated. > >Thanks in advance, >Sylinda Battle HT ASCP >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, inclusive of attachments, is intended for the person(s) stated above and may contain confidential information. Unauthorised reading, disclosure, copying, distribution or use of this information may violate rights to proprietary information. If you are not an intended recipient, please return this e-mail to the sender and delete your copy. Thank you. From trine.gejsing <@t> leo-pharma.com Fri Jul 14 00:24:15 2006 From: trine.gejsing <@t> leo-pharma.com (trine.gejsing@leo-pharma.com) Date: Fri Jul 14 00:49:18 2006 Subject: Fw: [Histonet] tissue loss Message-ID: ----- Forwarded by Trine Gejsing/Ballerup/DK/LEO on 14-07-2006 07:13 ----- Maybe you are boiling the slides "too hard". If the tissue contains a lot of collagen, that gives problems. Start out on full effect 900W or so. It would take like 9 min. When the buffer is boiling, immediatly set the effekt on 450W and cook for 25 min. I assume the tisuue is on Frost+ glass ? To histonet@lists.utsouthwestern.edu cc Subject [Histonet] tissue loss Hi - I'm trying a citrate buffer antigen retrieval protocol. After I have dewaxed the slides, I put them in citrate buffer - protocol says microwave on high for 25 minutes. However, when I remove the slides all the tissue has slid off the slide. Any suggestions? Melissa -- Melissa R. Mazan, DVM, Diplomate ACVIM Associate Professor of Large Animal Medicine Director of Sports Medicine Cummings School of Veterinary Medicine Tufts University 200 Westborough Road North Grafton, MA 01536 Tel: 508-839-5395 Fax: 508-839-7922 Email: melissa.mazan@tufts.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail, inclusive of attachments, is intended for the person(s) stated above and may contain confidential information. Unauthorised reading, disclosure, copying, distribution or use of this information may violate rights to proprietary information. If you are not an intended recipient, please return this e-mail to the sender and delete your copy. Thank you. From kemlo.rogerson <@t> waht.swest.nhs.uk Fri Jul 14 02:11:02 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Jul 14 02:10:22 2006 Subject: [Histonet] Non-histology question Message-ID: If there's any testosterone going FREE then I want it!!!!!! Why does your hair fall out when you get older when your testosterone gets 'weaker'? I'll ask my Chemical Pathology people as I am a recovering Histologist. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 I try to avoid looking forward or backward, and try to keep looking upward. --Charlotte Bronte This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From kemlo.rogerson <@t> waht.swest.nhs.uk Fri Jul 14 02:13:50 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Jul 14 02:13:10 2006 Subject: [Histonet] magnetized tissue?? Message-ID: What about wax when solid being heavier than molten wax? When you trim, you cool, then it's heavier therefore it sinks? What do you think???????? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 I try to avoid looking forward or backward, and try to keep looking upward. --Charlotte Bronte This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From muddymoo <@t> gmail.com Fri Jul 14 03:10:18 2006 From: muddymoo <@t> gmail.com (Alan Bishop) Date: Fri Jul 14 03:10:24 2006 Subject: [Histonet] magnetized tissue?? In-Reply-To: References: Message-ID: I was thinking the same thing. Cold blade solidifies the wax quicker on the cut end and hence that end sinks. Would seem to be the most logical reason. Alan On 14/07/06, Kemlo Rogerson wrote: > > What about wax when solid being heavier than molten wax? When you trim, > you > cool, then it's heavier therefore it sinks? > > What do you think???????? > > > From kemlo.rogerson <@t> waht.swest.nhs.uk Fri Jul 14 03:14:24 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Jul 14 03:13:47 2006 Subject: [Histonet] magnetized tissue?? Message-ID: But I said it first! Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 I try to avoid looking forward or backward, and try to keep looking upward. --Charlotte Bronte This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From muddymoo <@t> gmail.com Fri Jul 14 03:14:28 2006 From: muddymoo <@t> gmail.com (Alan Bishop) Date: Fri Jul 14 03:14:34 2006 Subject: [Histonet] magnetized tissue?? In-Reply-To: References: Message-ID: But you read my mind :-) On 14/07/06, Kemlo Rogerson wrote: > > But I said it first! > > > > Kemlo Rogerson > > Pathology Manager > > Ext 3311 > > DD 01934 647057 > > Mob 07749 754194 > > Pager 07659 597107 > > > > * * > > *I try to avoid looking forward or backward, and try to keep looking > upward. --Charlotte Bronte * > > > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on its > contents: to do so is strictly prohibited and may be unlawful. Please inform > me that this message has gone astray before deleting it. Thank you for your > co-operation > > > > > From muddymoo <@t> gmail.com Fri Jul 14 03:17:47 2006 From: muddymoo <@t> gmail.com (Alan Bishop) Date: Fri Jul 14 03:17:53 2006 Subject: [Histonet] magnetized tissue?? In-Reply-To: References: Message-ID: I was..... On 14/07/06, Kemlo Rogerson wrote: > > Wasn't wearing my glasses. > > > > Kemlo Rogerson > > Pathology Manager > > Ext 3311 > > DD 01934 647057 > > Mob 07749 754194 > > Pager 07659 597107 > > > > * * > > *I try to avoid looking forward or backward, and try to keep looking > upward. --Charlotte Bronte * > > > > This e-mail is confidential and privileged. If you are not the intended > recipient please accept my apologies; please do not disclose, copy or > distribute information in this e-mail or take any action in reliance on its > contents: to do so is strictly prohibited and may be unlawful. Please inform > me that this message has gone astray before deleting it. Thank you for your > co-operation > > > > > From oshel1pe <@t> cmich.edu Fri Jul 14 07:16:28 2006 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Fri Jul 14 07:16:36 2006 Subject: [Histonet] magnetized tissue?? In-Reply-To: <09C945920A6B654199F7A58A1D7D1FDE01717755@lsexch.lsmaster.lifespan.org> References: <09C945920A6B654199F7A58A1D7D1FDE01717755@lsexch.lsmaster.lifespan.org> Message-ID: 2 thoughts: One, you always drop the sample into the melted paraffin not-freshly-cut-end-first: cut end > X====| ^ < held toward freshly cut end So, the " | " end drops in first, vertically. Fluid dynamics will quickly flip a long, thin sample like this, unless it goes in vertically, so the freshly cut end lands pointing down. Easily tested: hold some specimens at one end and note if they land opposite-end down, then hold more specimens by the other end and repeat. Hold some sideways, and see how they land -- sideways, or one end perferentially. If it's not that, I'd bet on a static charge. All of the materials involved (specimen, paraffin) are insulators, so when you cut the end of the sample, you could be generating a static charge. If the metal embedding mold is on something conductive, or charged (-), and the induced charge is (+), then this might be able to flip the specimen. Assuming a dry, staticy environment. This could be tested with an anti-static gun, and zapping the specimen and mold. So, how long before this shows up on a CAP inspection? Phil >I have noticed an odd phenomenon many times, so I assume others must have >noticed it too? I often have slender, cylindrical specimens like vessels or >mouse spinal cord that have to be cut in cross section and therefore have to >be embedded "on end". Sometimes a specimen is a little too long to mount on >end without the cassette resting on top of the specimen. In such cases I >take a scalpel blade and trim a bit off the length, to make the specimen >"shorter". When I take a specimen that has been trimmed in this way, and >drop it back into the stainless steel embedding mold filled with paraffin, >the specimen instantly flips around and attaches on end to the bottom of the >mold, always by the freshly cut end, just as though it somehow became >"magnetized" by my trimming it. Has anyone else observed this? (Please say >yes, I can't be the only one!) If so, does anyone have any idea how/why >this occurs? Obviously it is not true magnetism operating here because (1) >tissue does not become magnetized, and (2) stainless steel is not attracted >to a magnet. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Philip Oshel Microscopy Facility Supervisor Department of Biology Central Michigan University 024C Brooks Hall Mt. Pleasant, MI 48859 voice: (989) 774-3576 dept. fax: (989) 774-3462 From TillRenee <@t> uams.edu Fri Jul 14 08:43:07 2006 From: TillRenee <@t> uams.edu (Till, Renee) Date: Fri Jul 14 08:43:34 2006 Subject: [Histonet] sectioning thymus Message-ID: <11F927674DEBDC43B960809A7403C5D2016F6363@MAILPED.ad.uams.edu> I have some thymus paraffin blocks that I am trying to section to do H&E on. I've never worked with this tissue before. What does it take to get decent sections? Most of the tissues I have cut worked fine by just soaking in ice with water. Sometimes I've even floated them on the water bath and that has helped. Neither of these seem to be enough to get these thymus blocks soft enough to get decent sections. I am already letting them soak for a couple hours. Should I go longer? Back when I was in histo school we used to soak the blocks in fabric softener, but so far even that hasn't worked. All my sections have chunks tore out like when the tissue is too dry, and now I am getting what looks like cracking. I have never had this much problems just cutting tissue. Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72002 Office (501)364-2785 Fax (501)364-3161 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From PMonfils <@t> Lifespan.org Fri Jul 14 09:48:31 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Jul 14 09:48:39 2006 Subject: [Histonet] sectioning thymus Message-ID: <09C945920A6B654199F7A58A1D7D1FDE01717756@lsexch.lsmaster.lifespan.org> Do you know what fixative was used on the thymus? If it was formalin fixed, the treatment you described should allow them to section well. However, I have found that tissues fixed in Histofix or similar fixatives sometimes have to be soaked overnight to 24 hours in order to get good sections, and even then I may get only 6 to 10 good sections before getting back into dry tissue. Make sure you know the fixative before trying this however, as soaking overnight could damage a formalin-fixed block. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Till, > Renee > Sent: Friday, July 14, 2006 6:43 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] sectioning thymus > > I have some thymus paraffin blocks that I am trying to section to do H&E > on. I've never worked with this tissue before. What does it take to get > decent sections? Most of the tissues I have cut worked fine by just > soaking in ice with water. Sometimes I've even floated them on the water > bath and that has helped. Neither of these seem to be enough to get > these thymus blocks soft enough to get decent sections. I am already > letting them soak for a couple hours. Should I go longer? Back when I > was in histo school we used to soak the blocks in fabric softener, but > so far even that hasn't worked. All my sections have chunks tore out > like when the tissue is too dry, and now I am getting what looks like > cracking. I have never had this much problems just cutting tissue. > > > > Renee' Till, HT > > Research Assistant > > Arkansas Children's Nutrition Center > > 1212 Marshall St./N2021 > > Little Rock, AR 72002 > > Office (501)364-2785 > > Fax (501)364-3161 > > > > > Confidentiality Notice: This e-mail message, including any attachments, is > for the sole use of the intended recipient(s) and may contain confidential > and privileged information. Any unauthorized review, use, disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From PMonfils <@t> Lifespan.org Fri Jul 14 11:26:21 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Jul 14 11:26:27 2006 Subject: [Histonet] magnetized tissue?? Message-ID: <09C945920A6B654199F7A58A1D7D1FDE01717757@lsexch.lsmaster.lifespan.org> Thanks for the various suggestions, but it is apparent that others have not seen this happening, which rather surprises me as I have seen it pretty regularly for years. What I described is definitely not due to any sort of fluid dynamics or the shape or density of the tissue sample. That might cause the tissue to float to the bottom of the mold a certain way. But what I am seeing is quite different. The tissue, once it is released into the liquid wax, and regardless of how it is oriented when released, instantly whips around to orient itself cut end down, and sticks fast to the bottom of the mold. I mean instantly, like a tenth of a second or less. If I pick it up with forceps and release it, it instantly reorients itself the same way. I agree with the one poster who suggested it must be some sort of static process, but I still can't understand how or why it happens. Anyway, it is not really a problem, just a matter of curiosity, and thanks again to all who responded. > ---------- > From: Rene J Buesa > Sent: Friday, July 14, 2006 9:01 AM > To: Monfils, Paul > Subject: Re: [Histonet] magnetized tissue?? > > Paul: > No, it never happened to me. > Consider the following explanation: > the cut end by the effect of being cut is squezed/compressed a little > making it slightly narrower/flatter than the non cut end. > When you place it back into the melted paraffin, by mere dynamics the > slightly flattened end will be less boyant than the other end, and will > sink faster. > Just a thought! > Ren? J. > > "Monfils, Paul" wrote: > > I have noticed an odd phenomenon many times, so I assume others must > have > noticed it too? I often have slender, cylindrical specimens like > vessels or > mouse spinal cord that have to be cut in cross section and therefore > have to > be embedded "on end". Sometimes a specimen is a little too long to > mount on > end without the cassette resting on top of the specimen. In such > cases I > take a scalpel blade and trim a bit off the length, to make the > specimen > "shorter". When I take a specimen that has been trimmed in this way, > and > drop it back into the stainless steel embedding mold filled with > paraffin, > the specimen instantly flips around and attaches on end to the > bottom of the > mold, always by the freshly cut end, just as though it somehow > became > "magnetized" by my trimming it. Has anyone else observed this? > (Please say > yes, I can't be the only one!) If so, does anyone have any idea > how/why > this occurs? Obviously it is not true magnetism operating here > because (1) > tissue does not become magnetized, and (2) stainless steel is not > attracted > to a magnet. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > _____ > > Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates > starting at 1?/min. > From PMonfils <@t> Lifespan.org Fri Jul 14 11:57:14 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Jul 14 11:57:20 2006 Subject: [Histonet] magnetized tissue?? Message-ID: <09C945920A6B654199F7A58A1D7D1FDE01717758@lsexch.lsmaster.lifespan.org> You mean not all the spirits are in the flammable cabinet?? From sbreeden <@t> nmda.nmsu.edu Fri Jul 14 13:03:26 2006 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Jul 14 13:03:34 2006 Subject: [Histonet] Full Moon Friday Message-ID: I think several ethereal spirits are at work here the last couple of days: hoof and toenail sections falling off slides, flagellating tubular sections affected by magnetism, and whatever the heck Kemlo was talking about. Breathe, people, breathe! It's FRIDAY! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87108 From crains <@t> wpmpath.com Fri Jul 14 13:29:25 2006 From: crains <@t> wpmpath.com (crains@wpmpath.com) Date: Fri Jul 14 13:29:31 2006 Subject: [Histonet] Full Moon Friday Message-ID: <20060714182927.BOEO3326.dukecmmtao03.coxmail.com@dukecmmtao03> It's bad luck to be superstitious. > > From: "Breeden, Sara" > Date: 2006/07/14 Fri PM 01:03:26 CDT > To: > Subject: [Histonet] Full Moon Friday > > I think several ethereal spirits are at work here the last couple of > days: hoof and toenail sections falling off slides, flagellating tubular > sections affected by magnetism, and whatever the heck Kemlo was talking > about. Breathe, people, breathe! It's FRIDAY! > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 700 > > Albuquerque, NM 87108 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From c.ingles <@t> hosp.wisc.edu Fri Jul 14 13:31:49 2006 From: c.ingles <@t> hosp.wisc.edu (Ingles Claire) Date: Fri Jul 14 13:32:33 2006 Subject: [Histonet] magnetized tissue?? References: <09C945920A6B654199F7A58A1D7D1FDE01717758@lsexch.lsmaster.lifespan.org> Message-ID: <583D3E9A1E843445BD54E461D1A2F6F317A04F6A@UWHIS-XCHNG2.uwhis.hosp.wisc.edu> Only the non-ethereal ones... :p ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Monfils, Paul Sent: Fri 7/14/2006 11:57 AM To: 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] magnetized tissue?? You mean not all the spirits are in the flammable cabinet?? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MElliott <@t> mrl.ubc.ca Fri Jul 14 13:59:31 2006 From: MElliott <@t> mrl.ubc.ca (Mark Elliott) Date: Fri Jul 14 13:59:53 2006 Subject: [Histonet] tissue loss Message-ID: <44B78723020000D600008450@mail.mrl.ubc.ca> My question with regards to this is, when I add my slides to the 95C buffer in the water bath, the temperature of the buffer drops because of the cold/room temperature slides-do you start timing the 20 mins then, or when the temp gets back up to 95? It takes awhile for the temp to get back to 95 (10+minutes). Mark >>> Rene J Buesa 07/13/06 11:49 AM >>> Melissa: This is the way I used to do HIER: heat the citrate buffer in the MWoven without the slides up to boliling point (time will depend on the energy output of yout MWoven and the amount of buffer). After that, put the heated citrate buffer in either a water bath (95?C) or a steamer (98?C) and when the hot citrate has thermally equilibrated with the hot environment into which it has been put (about 5 minutes) is then when you add the slides to do the HIER during 20 minutes; after those 20 minutes you take the container with the hot citrate buffer and the slides and place it over the counter for another 20 minutes to complete HIER. You will not lose sections with this procedure. Hope this will help you Ren? J. Melissa Mazan wrote: Hi - I'm trying a citrate buffer antigen retrieval protocol. After I have dewaxed the slides, I put them in citrate buffer - protocol says microwave on high for 25 minutes. However, when I remove the slides all the tissue has slid off the slide. Any suggestions? Melissa -- Melissa R. Mazan, DVM, Diplomate ACVIM Associate Professor of Large Animal Medicine Director of Sports Medicine Cummings School of Veterinary Medicine Tufts University 200 Westborough Road North Grafton, MA 01536 Tel: 508-839-5395 Fax: 508-839-7922 Email: melissa.mazan@tufts.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Sneak preview the all-new Yahoo.com. It's not radically different. Just radically better. From mhannah <@t> jhsph.edu Fri Jul 14 14:07:05 2006 From: mhannah <@t> jhsph.edu (Hannah, Michele F.) Date: Fri Jul 14 14:09:45 2006 Subject: [Histonet] RE: rat macrophage Ab References: <4uf7rh$1dnr96@gateway1.jhsph.edu> Message-ID: <1D71A10BB247204A9EFFB9EED3236058019168A2@XCH-VN02.sph.ad.jhsph.edu> This was previously mentioned, but I wanted to add that I use Serotec's ED1 antibody in the same rat tissue for both double fluorescent and non-fluorescent chromogenic staining. It has worked very well for us. M F. Hannah M.S. Department of Molecular Microbiology & Immunology Johns Hopkins Bloomberg School of Public Health 615 North Wolfe Street Room E3201 Baltimore, Maryland 21205 Phone: (410) 614-7794 Fax: (410) 955-0105 From ftryka <@t> tetonhospital.org Fri Jul 14 14:36:10 2006 From: ftryka <@t> tetonhospital.org (Dr. F. Tryka) Date: Fri Jul 14 14:36:15 2006 Subject: [Histonet] Leica microtome Message-ID: We are seeking advice. Our Leica 2125 RM microtome has been functioning well until it suddenly started cutting thick thin sections. We have tried to align it, etc and it still is not functioning properly. The block face looks smooth, but it will cut several slices toward one side, then on the next pass it cuts off a potato chip slice. Any suggestions would be appreciated. Franci Tryka From liz <@t> premierlab.com Fri Jul 14 14:56:07 2006 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Fri Jul 14 14:56:28 2006 Subject: [Histonet] Leica microtome In-Reply-To: Message-ID: <000401c6a77f$8c3dbfa0$0300a8c0@Chlipala> You need to get it serviced. We have ours serviced once a year. We have a 2145 and after 8 years we had to put a new motor in, but it might be something as simple as the lever that holds the plate on top of the disposable knife. We have had to replace that twice. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dr. F. Tryka Sent: Friday, July 14, 2006 1:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica microtome We are seeking advice. Our Leica 2125 RM microtome has been functioning well until it suddenly started cutting thick thin sections. We have tried to align it, etc and it still is not functioning properly. The block face looks smooth, but it will cut several slices toward one side, then on the next pass it cuts off a potato chip slice. Any suggestions would be appreciated. Franci Tryka _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RCHIOVETTI <@t> aol.com Fri Jul 14 14:59:24 2006 From: RCHIOVETTI <@t> aol.com (RCHIOVETTI@aol.com) Date: Fri Jul 14 14:59:40 2006 Subject: [Histonet] Leica microtome Message-ID: <3f6.1b20b800.31e9519c@aol.com> In a message dated 7/14/2006 12:36:59 PM US Mountain Standard Time, ftryka@tetonhospital.org writes: > We are seeking advice. Our Leica 2125 RM microtome has been > functioning well until it suddenly started cutting thick thin sections. > We have tried to align it, etc and it still is not functioning properly. > > The block face looks smooth, but it will cut several slices toward one > side, then on the next pass it cuts off a potato chip slice. Any > suggestions would be appreciated. Franci Tryka > > Franci, Most of the time when microtomes start acting up like this, there's a problem either with the clamping of the blade in the blade holder or with the knife holder itself. I'd check for paraffin or other debris between the clamping plates, a nick or a defect on the front or back clamping plate, the clamping pressure of the blade or the tightness of the knife holder where it clamps onto the base of the microtome. If all of that fails, you can contact Leica at 1-800-248-0123. Hope this helps! Cheers, Bob Robert (Bob) Chiovetti, Ph.D. Owner The Microscope Works Arizona's Microscopy Resource 132 North Elster Drive Tucson, AZ 85710-3212 USA Tel./Fax 520-546-4986 Manufacturers' Representative Member, Arizona Small Business Association - ASBA From RSRICHMOND <@t> aol.com Fri Jul 14 15:13:09 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Jul 14 15:13:16 2006 Subject: [Histonet] glyoxal fixation Message-ID: <495.5f4fea9.31e954d5@aol.com> The people at Anatech, makers of Prefer fixative, have published a review of glyoxal fixation that every pathologist and histotechnologist ought to read. This working surgical pathologist would like to add - and solicit - some comments on Histonet. "Glyoxal Fixation and Its Relationship to Immunohistochemistry". Richard W. Dapson, Ada T. Feldman, and Dee Wolfe. Anatech Limited, Battle Creek MI. The Journal of Histotechnology June 2006;29:65-76. I don't want to change, but I think we all need to be prepared for the day when a manager walks into our laboratory, or a letter from a regulatory agency arrives in the mail, telling us that we have to get rid of formaldehyde right now. Probably glyoxal is the only acceptable substitute, and we all need to have a look at it. I have a number of questions. Interchangeability of glyoxal products: Prefer is described as a buffered solution of glyoxal with a pH of about 4. The formula is a trade secret. Competing glyoxal products probably also have trade-secret formulas. So can a lab change brands of buffered glyoxal without problems, or does it have to stay with a particular brand with its trade-secret buffering? - We've seen a similar problem with distilling aliphatic xylene substitutes: every one of them requires a separate distillation routine, at least on a spinning-band still. - I'll leave it to John Kiernan to comment on the appropriateness of trade-secret reagents in histopathology. Limited time in the fixative: Tissue can be left in neutral buffered formalin for quite a long time and still be stainable, but tissue stored in glyoxal becomes unstainable after about two weeks. Can glyoxal fixed tissue be transferred to 70% ethanol for more prolonged storage? - A very occasional surgical specimen requires additional blocks after a week - a bigger problem will be the pathologist who doesn't trim his autopsies promptly. Transition period: A laboratory changing to glyoxal would have to keep IHC procedures for both fixatives working for some time. There would have to be some way to identify whether a block was fixed in formaldehyde or glyoxal. Eosinophilia: One ought to be able to distinguish eosinophils from neutrophils in tissue sections by nuclear morphology, without having to see granules. But quantitation of eosinophils - needed in an increasing number of GI biopsy situations - could be a problem. We might need an IHC for eosinophils in some of these settings. Lysis of erythrocytes: Not much of a problem, since we're used to it with acid fixatives anyway. Breast cancer: Elimination of nuclear bubble artifact in breast biopsy specimens may raise the apparent nuclear grade of tumors, and thus increase Nottingham (Elston-Ellis) scores. Prostate biopsies: I'd want to see some prostate biopsies - is somebody from OURLab in Nashville still on this list? - with formaldehyde fixation, nucleoli are a strong criterion of malignancy, and if glyoxal fixation demonstrates nucleoli in benign ductal epithelium, this criterion is lost. Bob Richmond Knoxville TN and Gastonia NC From Janet.Bonner <@t> flhosp.org Fri Jul 14 15:15:48 2006 From: Janet.Bonner <@t> flhosp.org (Bonner, Janet) Date: Fri Jul 14 15:16:40 2006 Subject: [Histonet] Full Moon Friday References: Message-ID: Kemlo was talking to himself, I think......... ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Breeden, Sara Sent: Fri 7/14/2006 2:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Full Moon Friday I think several ethereal spirits are at work here the last couple of days: hoof and toenail sections falling off slides, flagellating tubular sections affected by magnetism, and whatever the heck Kemlo was talking about. Breathe, people, breathe! It's FRIDAY! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87108 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cwscouten <@t> myneurolab.com Fri Jul 14 15:18:46 2006 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Fri Jul 14 15:18:58 2006 Subject: [Histonet] Leica microtome Message-ID: <5784D843593D874C93E9BADCB87342AB013072DA@tpiserver03.Coretech-holdings.com> Usually, when we hear about such things, the answer is the blade angle is too shallow, or the pedestal has wobble for some reason. Grasp the tissue pedastal with your hand and try to wobble it. If you can, that is the problem. Has the blade angle changed, on purpose or by accident? Also try to wiggle the blade to see if it is loose, but try to do that without losing a hand. Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dr. F. Tryka Sent: Friday, July 14, 2006 2:36 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica microtome We are seeking advice. Our Leica 2125 RM microtome has been functioning well until it suddenly started cutting thick thin sections. We have tried to align it, etc and it still is not functioning properly. The block face looks smooth, but it will cut several slices toward one side, then on the next pass it cuts off a potato chip slice. Any suggestions would be appreciated. Franci Tryka _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Jul 14 16:13:23 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jul 14 16:13:27 2006 Subject: [Histonet] Leica microtome In-Reply-To: Message-ID: <20060714211323.97098.qmail@web61221.mail.yahoo.com> Do not try to solve this problem. See help from Leica people and have it services. This type of microtome should be professionally services annually. Hope this will help you.Have anice weekend. Ren? J. "Dr. F. Tryka" wrote: We are seeking advice. Our Leica 2125 RM microtome has been functioning well until it suddenly started cutting thick thin sections. We have tried to align it, etc and it still is not functioning properly. The block face looks smooth, but it will cut several slices toward one side, then on the next pass it cuts off a potato chip slice. Any suggestions would be appreciated. Franci Tryka _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail Beta. From adafeldman <@t> anatechltdusa.com Fri Jul 14 16:24:13 2006 From: adafeldman <@t> anatechltdusa.com (Ada Feldman) Date: Fri Jul 14 16:24:49 2006 Subject: [Histonet] glyoxal fixation In-Reply-To: <495.5f4fea9.31e954d5@aol.com> References: <495.5f4fea9.31e954d5@aol.com> Message-ID: As the developers of the Prefer fixative we would like to address some of the issues. Interchangeability of glyoxal products: The manufacturer of each fixative should be able to provide the information necessary to work with their fixative in conjunction with any other fixative that a lab may be using. Just as an example, Hollandes is not compatible with NBF and requires special attention. As for Prefer we can say it is compatible with the majority of other fixatives, glyoxal or not. Limited time in the fixative: There is a slight reduction in staining intensity after several weeks, but increasing staining time corrects this. So tissues are not rendered unstainable. Transition period: This is true any time you are switching fixatives or processing methods. Eosinophila: Your statements here are true. Lysis of erythrocytes: True again. It is often seen as an advantage because diagnostics cells are easier to see. Breast cancer: It would seem that the improved nuclear morphology would make marked nuclear variation easier to determine. Prostate biopsies: Hope you can get a response from any of the glyoxal users with prostate biopsies. We would be interested in this answer also. Ada T. Feldman, MS, HT/HTL(ASCP) ANATECH LTD. 1020 Harts Lake Road Battle Creek, MI 49015 Phone: 800.262.8324 Fax: 269.964.8084 email: adafeldman@anatechltdusa.com website: www.anatechltdusa.com On Jul 14, 2006, at 4:13 PM, RSRICHMOND@aol.com wrote: > The people at Anatech, makers of Prefer fixative, have published a > review of > glyoxal fixation that every pathologist and histotechnologist ought > to read. > This working surgical pathologist would like to add - and solicit - > some > comments on Histonet. > > "Glyoxal Fixation and Its Relationship to Immunohistochemistry". > Richard W. > Dapson, Ada T. Feldman, and Dee Wolfe. Anatech Limited, Battle > Creek MI. The > Journal of Histotechnology June 2006;29:65-76. > > I don't want to change, but I think we all need to be prepared for > the day > when a manager walks into our laboratory, or a letter from a > regulatory agency > arrives in the mail, telling us that we have to get rid of > formaldehyde right > now. Probably glyoxal is the only acceptable substitute, and we all > need to > have a look at it. I have a number of questions. > > Interchangeability of glyoxal products: Prefer is described as a > buffered > solution of glyoxal with a pH of about 4. The formula is a trade > secret. > Competing glyoxal products probably also have trade-secret > formulas. So can a lab > change brands of buffered glyoxal without problems, or does it have > to stay with a > particular brand with its trade-secret buffering? - We've seen a > similar > problem with distilling aliphatic xylene substitutes: every one of > them requires a > separate distillation routine, at least on a spinning-band still. - > I'll > leave it to John Kiernan to comment on the appropriateness of trade- > secret > reagents in histopathology. > > Limited time in the fixative: Tissue can be left in neutral > buffered formalin > for quite a long time and still be stainable, but tissue stored in > glyoxal > becomes unstainable after about two weeks. Can glyoxal fixed tissue be > transferred to 70% ethanol for more prolonged storage? - A very > occasional surgical > specimen requires additional blocks after a week - a bigger problem > will be the > pathologist who doesn't trim his autopsies promptly. > > Transition period: A laboratory changing to glyoxal would have to > keep IHC > procedures for both fixatives working for some time. There would > have to be some > way to identify whether a block was fixed in formaldehyde or glyoxal. > > Eosinophilia: One ought to be able to distinguish eosinophils from > neutrophils in tissue sections by nuclear morphology, without > having to see granules. > But quantitation of eosinophils - needed in an increasing number of > GI biopsy > situations - could be a problem. We might need an IHC for > eosinophils in some of > these settings. > > Lysis of erythrocytes: Not much of a problem, since we're used to > it with > acid fixatives anyway. > > Breast cancer: Elimination of nuclear bubble artifact in breast biopsy > specimens may raise the apparent nuclear grade of tumors, and thus > increase > Nottingham (Elston-Ellis) scores. > > Prostate biopsies: I'd want to see some prostate biopsies - is > somebody from > OURLab in Nashville still on this list? - with formaldehyde > fixation, nucleoli > are a strong criterion of malignancy, and if glyoxal fixation > demonstrates > nucleoli in benign ductal epithelium, this criterion is lost. > > Bob Richmond > Knoxville TN and Gastonia NC > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet Ada T. Feldman, MS, HT/HTL(ASCP) ANATECH LTD. 1020 Harts Lake Road Battle Creek, MI 49015 Phone: 800.262.8324 Fax: 269.964.8084 email: adafeldman@anatechltdusa.com website: www.anatechltdusa.com From Jackie.O'Connor <@t> abbott.com Fri Jul 14 16:55:24 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Jul 14 16:55:52 2006 Subject: [Histonet] Job posting - North Chicago, IL In-Reply-To: <20060714211323.97098.qmail@web61221.mail.yahoo.com> Message-ID: This is a great opportunity! Please direct inquiries to Alex.Shoemaker@abbott.com. Abbott is committed to the discovery and development of innovative treatments to help patients in the fight against cancer. Abbott is at the forefront of cancer research in discovering and developing novel treatments that offer a new approach to cancer therapy. Our strong pipeline includes signal transduction inhibitors as well as first in class agents that target angiogenesis, metastasis and resistance to apoptosis. Abbott?s oncology franchise extends beyond pharmaceutical research to provide a range of health care products, from supportive care products for pain management, to diagnostic tests for the detection of cancer. Our expanding In Vivo Tumor Biology group and has an opening for a motivated individual who is dedicated to the discovery of new medicines for the treatment of cancer. This team conducts cutting-edge research to characterize antibody and small molecule therapeutics from an array of oncology programs. Our scientists are integral members of drug-discovery teams who interface with both internal and external biomarker, toxicology, proteomics and genomics groups to deliver novel and effective clinical candidates. Located in the Chicago, IL area, we have the following opening: Histology Associate Individual will join a tumor biology team to conduct tissue processing, sectioning and staining procedures. Requires either a BS degree in biology or Associate degree with 2-5 years histology experience. Experience with tissue collection and preparation for routine staining, tissue processing, embedding, sectioning (paraffin and cryostat), microscopy and experience with conducting established IHC stains (e.g. H&E, caspase, Ki67) as well as optimizing new antibodies, are required. ASCP-HT certification and animal pharmacology experience are a plus. Strong organizational, record keeping and computer skills are essential. ( Job code 34469BR) For more details on each position and consideration, please visit www.abbott.com. Click the Career Center, Job Opportunities, Search Openings, Enter 34469BR listed into the Keyword field. From melissa.mazan <@t> tufts.edu Sat Jul 15 06:06:32 2006 From: melissa.mazan <@t> tufts.edu (Melissa Mazan) Date: Sat Jul 15 06:06:39 2006 Subject: [Histonet] tissue loss Message-ID: <20060715070632.5hols25lwwocg8ks@webmail.tufts.edu> Thanks to all who gave very helpful suggestions. The slides are Star Frost plus, I'm trying the pressure cooker protocol - hoping for better results. Melissa From kemlo.rogerson <@t> waht.swest.nhs.uk Mon Jul 17 02:22:20 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Jul 17 02:21:47 2006 Subject: [Histonet] sectioning thymus Message-ID: "I have some thymus paraffin blocks that I am trying to section to do > H&E on. I've never worked with this tissue before. What does it take > to get decent sections? Most of the tissues I have cut worked fine by > just soaking in ice with water. Sometimes I've even floated them on > the water bath and that has helped." I'm confused......... these are paraffin blocks aren't they? If so then why take all that time getting the water out to soak them in the stuff again? Thymus from memory is very "nucleary" or 'dense' and I had problems with poorly fixed, poorly processed tissue. Solution (forgive the pun) properly fix and then properly process; do not go near water again until the staining part has been reached. To cool use those plastic thingies you use to cool your tinnies. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 I try to avoid looking forward or backward, and try to keep looking upward. --Charlotte Bronte This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From hilsley <@t> capeheart.uct.ac.za Mon Jul 17 04:14:49 2006 From: hilsley <@t> capeheart.uct.ac.za (Helen Ilsley) Date: Mon Jul 17 04:17:31 2006 Subject: [Histonet] fibroblast marker Message-ID: Hi I wonder if anyone can help me. I am looking for a fibroblast marker which can cross react with any of the following: rat, rabbit or baboon. I have done quite a few searches and come up with nothing. Thanks in advance, Helen Ilsley From SMulla <@t> wockhardtin.com Mon Jul 17 05:02:57 2006 From: SMulla <@t> wockhardtin.com (SMulla@wockhardtin.com) Date: Mon Jul 17 05:00:27 2006 Subject: [Histonet] (no subject) Message-ID: Dear all we get very good result for histochemistry of alkaline phosphatase in kidney section. But I could not get any deposits for the same in liver section. Write if you have any idea. Thanks in advance. Please Visit our New Corporate Web Site www.wockhardt.com --------------------- Disclaimer ------------------------------------------------------------------------------ Information transmitted by this E-MAIL is proprietary to Wockhardt Ltd. and/ or its Group Companies and/or,its Customers and is intended for use only by the individual or entity to which it is addressed, and may contain information that is privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. --------------------------------------------------------------------------------------------------------------- From liz <@t> premierlab.com Mon Jul 17 10:29:19 2006 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Mon Jul 17 10:56:16 2006 Subject: [Histonet] fibroblast marker In-Reply-To: Message-ID: <001101c6a9b5$c5c3b530$0300a8c0@Chlipala> Helen There is a marker called prolyl-4-hydroxylase that is supposed to react with fibroblasts. Its from Acris cat number AF5110-1. The antibody is a mouse anti-rat. It is supposed to work in FFPE material. I just received it and have not had a change to start working on it, but I can update as soon as I start working with it. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Helen Ilsley Sent: Monday, July 17, 2006 3:15 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] fibroblast marker Hi I wonder if anyone can help me. I am looking for a fibroblast marker which can cross react with any of the following: rat, rabbit or baboon. I have done quite a few searches and come up with nothing. Thanks in advance, Helen Ilsley _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Mon Jul 17 13:21:03 2006 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Mon Jul 17 17:21:22 2006 Subject: [Histonet] re: fiboblast marker Message-ID: I understand that a Vimentin Ab will suit your needs? If you go here http://www.immunoportal.com/index.php , hit the Image gallery link on the left and type in "vimentin" in the search box ( top right). Enter and you should find three pics. Sure, two are of Astrocytes but I also use the anti Vimentin ( V9) Ab to stain rat fibroblasts/cytes in non-neuronal p.wax sections. It does not react with mouse and I do not know if it is baboon/rabbit reactive. Hope I am correct and it is helpful carl From TraceyG <@t> adhb.govt.nz Mon Jul 17 17:39:31 2006 From: TraceyG <@t> adhb.govt.nz (Tracey Gunn) Date: Mon Jul 17 17:39:41 2006 Subject: [Histonet] BAF47 (Ini1) antibody Message-ID: Hello, Is anyone successfully using this antibody on formalin-fixed, paraffin embedded tissue? Thanks, Tracey Gunn. Tracey Gunn, Section Leader Histology, LabPlus, Auckland City Hospital, Auckland, New Zealand. From dendritica <@t> gmail.com Mon Jul 17 20:16:34 2006 From: dendritica <@t> gmail.com (Dendritica) Date: Mon Jul 17 20:16:41 2006 Subject: [Histonet] Forms of 3,3'-Diaminobenzidine (DAB) Message-ID: <001b01c6aa07$cfd084c0$5a3c40ab@Sarina> Greetings! Histonet newbie here. I recently ordered 3,3'-Diaminobenzidine (Sigma cat. # D8001), not realizing that I have always used the tetrahydrochloride form of DAB in the past, with readily dissolves in water. I am wondering if I can simply dissolve the DAB I ordered in HCl and perform my experiments as usual (the DAB I ordered does not dissolve in water or PBS, but does in HCl). I used to freeze dissolved DAB in aliquots with the water-soluable form and am curious if I can do the same with the HCl-soluable form. FYI, I use it to immunohistochemically stain brain tissue. Any advice on how to differently handle the two forms of DAB would be greatly appreciated. Thanks so much for your input! :) Mia From C.E.Savage <@t> liverpool.ac.uk Tue Jul 18 06:18:24 2006 From: C.E.Savage <@t> liverpool.ac.uk (carol savage) Date: Tue Jul 18 06:18:36 2006 Subject: [Histonet] IFA on FFPE tissue Message-ID: Dear All Please can anyone help by sharing with me tips on Immunoflorescent staining on formalin-fixed, paraffin embedded tissue on poly-L-lysine slides. We are trying to stain avian tissue using an inhouse produced anti sera followed by a commercial florescent conjugate which has worked well on cryostat cut sections. Many thanks Carol Savage Mrs. C. E. Savage Senior Technician Veterinary Pathology University of Liverpool E.mail c.e.savage@liv.ac.uk Tel 0151 794 6111 Fax 0151 784 6110 From mauger <@t> email.chop.edu Tue Jul 18 06:53:05 2006 From: mauger <@t> email.chop.edu (Joanne Mauger) Date: Tue Jul 18 07:13:49 2006 Subject: [Histonet] BAF47 (Ini1) antibody Message-ID: Tracey, It works well. I use high pH antigen retrieval(pH10), dilution 1:250. Jo Mauger >>> "Tracey Gunn" 07/17/06 6:39 PM >>> Hello, Is anyone successfully using this antibody on formalin-fixed, paraffin embedded tissue? Thanks, Tracey Gunn. Tracey Gunn, Section Leader Histology, LabPlus, Auckland City Hospital, Auckland, New Zealand. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kbroomal <@t> NEMOURS.ORG Tue Jul 18 08:24:01 2006 From: kbroomal <@t> NEMOURS.ORG (Kristen Broomall) Date: Tue Jul 18 08:24:25 2006 Subject: [Histonet] Technovit 9100 Help! Message-ID: <6E41111281623B4B8A9AB8F9A7EA34370EE4DFDE@wlmmsx02.nemours.org> Could any of you experts at using Technovit 9100 neu drop me an email? I'm using it for the first time on mouse anterior tibias & am having a horrible time getting the polymerization mixture to polymerize. I'm sure tech support is getting tired of me calling them (haha..). I'm looking for some "real life users" of this stuff to figure out what I'm doing wrong. Kristen Broomall, HT (ASCP) AI duPont Hospital for Children Histotechnology Core Lab Lab: 302-651-6771 Fax: 302-651-5010 kbroomal@nemours.org From coleman_manufacturing <@t> yahoo.com Tue Jul 18 08:41:56 2006 From: coleman_manufacturing <@t> yahoo.com (Coleman Manufacturing) Date: Tue Jul 18 08:42:00 2006 Subject: [Histonet] Fisher Scientific products Message-ID: <20060718134156.70543.qmail@web37601.mail.mud.yahoo.com> Hello everyone! Let me first say I hope this message does not make anyone mad, for I know HistoNet was intended for educational and information sharing purposes only. With this said, I am Justin Coleman with Coleman Manufacturing out of South Carolina. Just wanted to post a message to everyone informing all that CMD is now a direct distributor for Fisher Scientific. We distribute any and all products from Fisher. For example, we distribute laboratory equipment (Microwaves, cryostats, etc.), laboratory reagents, special stains, and disposables. Please do not hesitate to contact us if we can help you out; maybe we can do better on pricing for you! Thanks to all, Justin Coleman Coleman Manufacturing & Design South Carolina 803.633.2124 office 803.635-9401 fax From ttroyer <@t> petersonlab.com Tue Jul 18 09:48:24 2006 From: ttroyer <@t> petersonlab.com (Travis Troyer) Date: Tue Jul 18 09:48:27 2006 Subject: [Histonet] parafin on fabric chairs Message-ID: <000801c6aa79$39023ad0$6601010a@Peterson.local> We have some fabric chairs in our lab that have parafin on them. Does anyone know of a way to clean the wax off? Thanks Travis From rjr6 <@t> psu.edu Tue Jul 18 09:55:19 2006 From: rjr6 <@t> psu.edu (Roberta Horner) Date: Tue Jul 18 09:55:25 2006 Subject: [Histonet] parafin on fabric chairs In-Reply-To: <000801c6aa79$39023ad0$6601010a@Peterson.local> Message-ID: This works on carpeting so you may want to try it. You need a brown paper bag and an iron. Put the paper on the chair and run the hot iron over the paper. The iron will melt the paraffin and the paper should absorb the paraffin. Roberta -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Travis Troyer Sent: Tuesday, July 18, 2006 10:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] parafin on fabric chairs We have some fabric chairs in our lab that have parafin on them. Does anyone know of a way to clean the wax off? Thanks Travis _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Jul 18 09:59:40 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jul 18 09:59:44 2006 Subject: [Histonet] parafin on fabric chairs In-Reply-To: Message-ID: <20060718145940.51398.qmail@web61221.mail.yahoo.com> But you will have to repeat this many, many, many times (and it is likely that there will always be a residue noticeable as a different colour/texture). That is what happened to me! Ren? J. Roberta Horner wrote: This works on carpeting so you may want to try it. You need a brown paper bag and an iron. Put the paper on the chair and run the hot iron over the paper. The iron will melt the paraffin and the paper should absorb the paraffin. Roberta -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Travis Troyer Sent: Tuesday, July 18, 2006 10:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] parafin on fabric chairs We have some fabric chairs in our lab that have parafin on them. Does anyone know of a way to clean the wax off? Thanks Travis _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. From Jackie.O'Connor <@t> abbott.com Tue Jul 18 09:59:12 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Tue Jul 18 09:59:47 2006 Subject: [Histonet] paraffin on fabric chairs In-Reply-To: <000801c6aa79$39023ad0$6601010a@Peterson.local> Message-ID: Not a lab solution, but a household one - -you can iron it off by placing a material like a pillowcase on the chair and using a household iron, melt the paraffin so it is absorbed into the pillowcase. In most labs, fabric chairs are not allowed due to the potential for absorbing hazardous chemicals, biohazards, etc. Lab chairs should be made of non-absorbent material. Use this to get your safety department to spring for new chairs - preferably, ergonomic chairs. Jackie O' "Travis Troyer" Sent by: histonet-bounces@lists.utsouthwestern.edu 07/18/2006 09:48 AM To cc Subject [Histonet] parafin on fabric chairs We have some fabric chairs in our lab that have parafin on them. Does anyone know of a way to clean the wax off? Thanks Travis _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DavidH <@t> marketlabinc.com Tue Jul 18 10:15:16 2006 From: DavidH <@t> marketlabinc.com (David Haagsma) Date: Tue Jul 18 10:15:29 2006 Subject: [Histonet] parafin on fabric chairs Message-ID: <19E3602A16438E48B51A4250CA04B5F67530F1@exchange.marketlab.com> Travis, I have heard that Goo-Gone will work for this, and for paraffin clean up in general. I have absolutely no proof for this but it is worth a try as you can get goo-gone pretty cheap from Wal-mart. Dave Haagsma Research Market Manager MarketLab Inc. 6850 Southbelt Dr Caledonia MI 49316 800-237-3604 Fax 616-656-2475 Unique and Hard to Find Products for your Laboratory http://www.marketlabinc.com/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Travis Troyer Sent: Tuesday, July 18, 2006 10:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] parafin on fabric chairs We have some fabric chairs in our lab that have parafin on them. Does anyone know of a way to clean the wax off? Thanks Travis _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lblazek <@t> digestivespecialists.com Tue Jul 18 10:21:45 2006 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Jul 18 10:17:45 2006 Subject: [Histonet] parafin on fabric chairs Message-ID: <6CBA6DC98A079D408C87250591D9DFB802360DB6@bruexchange.digestivespecialists.com> Oh heck........ Start by scraping off as much as you can then get a gauze really soaked with xylene and scrub the rest out. (leave me alone, it's not Friday yet!) Linda -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, July 18, 2006 11:00 AM To: Roberta Horner; Travis Troyer; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] parafin on fabric chairs But you will have to repeat this many, many, many times (and it is likely that there will always be a residue noticeable as a different colour/texture). That is what happened to me! Ren? J. Roberta Horner wrote: This works on carpeting so you may want to try it. You need a brown paper bag and an iron. Put the paper on the chair and run the hot iron over the paper. The iron will melt the paraffin and the paper should absorb the paraffin. Roberta -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Travis Troyer Sent: Tuesday, July 18, 2006 10:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] parafin on fabric chairs We have some fabric chairs in our lab that have parafin on them. Does anyone know of a way to clean the wax off? Thanks Travis _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- How low will we go? Check out Yahoo! Messenger's low PC-to-Phone call rates. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Tue Jul 18 11:32:03 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Jul 18 11:32:17 2006 Subject: [Histonet] Flt-1 and Flk-1 Message-ID: <000001c6aa87$b6eb2b10$eeeea8c0@SERVER01> Hi Histonetters, Flt-1 and Flk-1: Is anybody successfull with ihc-staining of these two antibodies (human tissue)? I use the Ventana benchmark XT (CC1 mild, 1:40, 40 min). Untill now I've got nuclear staining instead of cytoplasmic in placenta. Any hints are appreciated. Gudrun Lang From histology <@t> gradymem.org Tue Jul 18 11:49:04 2006 From: histology <@t> gradymem.org (histology@gradymem.org) Date: Tue Jul 18 11:52:33 2006 Subject: [Histonet] necrosis detection Message-ID: <80534480211c.80211c805344@onenet.net> My pathologist has a question: After cell death occurs, what is the earlist time at which necrosis can be detected by H&E stains? Thanks, Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org From ploykasek <@t> phenopath.com Tue Jul 18 12:29:14 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Tue Jul 18 12:29:26 2006 Subject: [Histonet] Cdx clone 88 Message-ID: Hi all. I just heard that Biogenex is discontinuing cdx-2 clone 88. Does anyone know of an alternate source for this antibody, I prefer concentrates? I do know that BioCare carries a predilute. I appreciate the help. For any vendors out there, it would be nice if you would inform your customers when you are going to discontinue a product. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From Rcartun <@t> harthosp.org Tue Jul 18 12:56:41 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Jul 18 12:57:20 2006 Subject: [Histonet] Cdx clone 88 In-Reply-To: References: Message-ID: <44BCE8990200007700000F80@hcnwgwds01.hh.chs> Good point Patti. Dako recently discontinued several infectious disease antibodies that I use and I didn't find out about until we tried to order more antibody after we ran out. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> Patti Loykasek 07/18/06 1:29 PM >>> Hi all. I just heard that Biogenex is discontinuing cdx-2 clone 88. Does anyone know of an alternate source for this antibody, I prefer concentrates? I do know that BioCare carries a predilute. I appreciate the help. For any vendors out there, it would be nice if you would inform your customers when you are going to discontinue a product. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> flhosp.org Tue Jul 18 13:07:44 2006 From: Janet.Bonner <@t> flhosp.org (Bonner, Janet) Date: Tue Jul 18 13:11:51 2006 Subject: [Histonet] parafin on fabric chairs References: Message-ID: We had to cover all of our fabric chairs with vinyl and all of the new chairs need to be vinyl. I'm not sure, but I believe this is regulation! Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Roberta Horner Sent: Tue 7/18/2006 10:55 AM To: Travis Troyer; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] parafin on fabric chairs This works on carpeting so you may want to try it. You need a brown paper bag and an iron. Put the paper on the chair and run the hot iron over the paper. The iron will melt the paraffin and the paper should absorb the paraffin. Roberta -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Travis Troyer Sent: Tuesday, July 18, 2006 10:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] parafin on fabric chairs We have some fabric chairs in our lab that have parafin on them. Does anyone know of a way to clean the wax off? Thanks Travis _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bjdewe <@t> aol.com Tue Jul 18 13:13:45 2006 From: bjdewe <@t> aol.com (bjdewe@aol.com) Date: Tue Jul 18 13:13:58 2006 Subject: [Histonet] IHC Animal Tissue workshop Message-ID: <8C878AD0B33763B-1E98-32D9@FWM-D38.sysops.aol.com> Due to the high demand we have decided to have another Animal Tissue Workshop on Aug 25, 2006. If you would like to attend this hands on wet workshop please sign up at the link on the flyer. http://www.vetmed.ucdavis.edu/vsr/dil/events.html Cheers, Loralei Dewe UC Davis School of Veterinary Medicine Davis, Ca Live as if you were to die tomorrow. Learn as if you were to live forever. - Gandhi - ******************************************************************* Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s)and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ******************************************************************* ________________________________________________________________________ Check out AOL.com today. Breaking news, video search, pictures, email and IM. All on demand. Always Free. From melissa.mazan <@t> tufts.edu Tue Jul 18 13:16:39 2006 From: melissa.mazan <@t> tufts.edu (Melissa Mazan) Date: Tue Jul 18 13:16:44 2006 Subject: [Histonet] tissue loss Message-ID: <20060718141639.0r6708ik0sw4o8cs@webmail.tufts.edu> Hi all, just wanted to let all the helpful people know that the problem was in the ph of the buffer. It was far too alkaline - once I corrected the pH the tissues stayed nicely on the slides. Many thanks - Melissa From LRaff <@t> lab.uropartners.com Tue Jul 18 13:23:14 2006 From: LRaff <@t> lab.uropartners.com (Lester Raff) Date: Tue Jul 18 13:23:20 2006 Subject: [Histonet] PIN Cocktails Message-ID: <5DA1CA5D0B98A84985B545A24423B82201989F@UPLAB01.uplab.local> HI All: We are currently running the BioCare PIN 2 Cocktail on the Dako autostainer and do a separate HMW keratin. A consultant has recommended going to the PIN 4. On looking at BioCare's antibody data sheet, I see that they have 2 different PIN 4's, one of which requires their Double Vision double stain. What is the difference between these, and what have people's experiences been with this cocktail? Thanks! Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 From godsgirlnow <@t> msn.com Tue Jul 18 13:28:31 2006 From: godsgirlnow <@t> msn.com (Roxanne Soto) Date: Tue Jul 18 13:28:45 2006 Subject: [Histonet] PIN Cocktails In-Reply-To: <5DA1CA5D0B98A84985B545A24423B82201989F@UPLAB01.uplab.local> Message-ID: I use the PIN-4 cocktail about 100 times a week, at least and the doctors here love it. But in addition to using the Mach 2 double stain kit, you also need a second chromagen, if you aren't already using one. Roxanne Soto Physicians RightPath Tampa, Florida ______________________________________________________________ From: "Lester Raff" To: Subject: [Histonet] PIN Cocktails Date: Tue, 18 Jul 2006 13:23:14 -0500 >HI All: > > > >We are currently running the BioCare PIN 2 Cocktail on the Dako >autostainer and do a separate HMW keratin. A consultant has recommended >going to the PIN 4. On looking at BioCare's antibody data sheet, I see >that they have 2 different PIN 4's, one of which requires their Double >Vision double stain. What is the difference between these, and what >have people's experiences been with this cocktail? > > > >Thanks! > > > >Lester J. Raff, MD >Medical Director >UroPartners, LLC Laboratory > >2225 Enterprise Dr. Suite 2511 > >Westchester, IL 60154 > > >ph: 708-486-0076 >fax: 708-486-0080 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From m.wei <@t> biogenex.com Tue Jul 18 13:31:17 2006 From: m.wei <@t> biogenex.com (May Wei) Date: Tue Jul 18 13:31:22 2006 Subject: [Histonet] Cdx clone 88 Message-ID: <37DC9F93CF7F864182D0463EF93D571B206420@ISLETON2.california.biogenex.com> Dear Patti, CDX-2 clone 88 is still available in BioGenex. The concentrated format is MU392A-UC 1ml size, and the Ready-To-Use format is AM392-5M 6ml size or AM392-10M 10ml size with barcode label. Thanks. May Wei, M.Med. BioGenex Laboratories Tel: 1 800 421-4149 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Tuesday, July 18, 2006 10:29 AM To: histonet Subject: [Histonet] Cdx clone 88 Hi all. I just heard that Biogenex is discontinuing cdx-2 clone 88. Does anyone know of an alternate source for this antibody, I prefer concentrates? I do know that BioCare carries a predilute. I appreciate the help. For any vendors out there, it would be nice if you would inform your customers when you are going to discontinue a product. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------ - This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Heather.A.Harper <@t> pcola.med.navy.mil Tue Jul 18 13:57:52 2006 From: Heather.A.Harper <@t> pcola.med.navy.mil (Heather.A.Harper@pcola.med.navy.mil) Date: Tue Jul 18 14:04:40 2006 Subject: [Histonet] Microtome blades Message-ID: Hi Everybody, I just wanted to thank every body for their input when I was having serious problems getting decent paraffin ribbons when cutting on the microtome. I tried like 5 different brands of high profile blades, tried different angles, and concluded it had to be the knife holder or the block holder. Called in the rep, and he was baffled. Finally he used my co-worker's, blade holder and could cut beautifully. So now I have a new knife holder and have switched to low profile Accu Edge blades, which cut fabulously. Thanks for helping me trouble shoot. Heather A. Harper Naval Hospital Pensacola, FL From pathrm35 <@t> adelphia.net Tue Jul 18 14:09:40 2006 From: pathrm35 <@t> adelphia.net (pathrm35@adelphia.net) Date: Tue Jul 18 14:09:44 2006 Subject: [Histonet] Histotech and MOHS tech postions in SE Florida Message-ID: <9217866.1153249780796.JavaMail.root@web17> We are currently seeking one F/T, HT/HTL for our dermpath lab in Boca Raton, Florida. This is a new lab currently being built with brand new equipment on order. Duties include gross, embed, section, IHC and special stains on derm cases only. This position requires a Florida license as a Histology Technologist. We are also seeking an experienced MOHS tech/histotech for our North Miami/ Fort Lauderdale areas. Experience and ASCP/Florida licensing preferred. Ron Martin From rjbuesa <@t> yahoo.com Tue Jul 18 15:25:07 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jul 18 15:25:11 2006 Subject: [Histonet] necrosis detection In-Reply-To: <80534480211c.80211c805344@onenet.net> Message-ID: <20060718202507.12459.qmail@web61222.mail.yahoo.com> Angie: Run a little expriment by leaving unfixed tissue during at least 8 hours. Fix as usual after half an hour intervals (16 in total) and process the tissue. Section and stain with H&E and let your pathologist look for the signs of necrosis in each slide from each time interval. If they are not evident, increase to more time in same time length intervals. I think this would be a good way to find out. Hope this will help you! Ren? J. histology@gradymem.org wrote: My pathologist has a question: After cell death occurs, what is the earlist time at which necrosis can be detected by H&E stains? Thanks, Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail Beta. From ploykasek <@t> phenopath.com Tue Jul 18 16:52:57 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Tue Jul 18 16:53:08 2006 Subject: [Histonet] Flt-1 and Flk-1 In-Reply-To: <000001c6aa87$b6eb2b10$eeeea8c0@SERVER01> Message-ID: Hi Gudrun. Our research department has worked with Flt-1 about a year ago. For the Flt-1 (VEGF R-1) they use a rabbit polyclonal from Neomarkers with a heat citrate pretreatment. We do not have any experience with Flk-1. Hope this helps. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Hi Histonetters, > Flt-1 and Flk-1: Is anybody successfull with ihc-staining of these two > antibodies (human tissue)? I use the Ventana benchmark XT (CC1 mild, 1:40, > 40 min). > Untill now I've got nuclear staining instead of cytoplasmic in placenta. > Any hints are appreciated. > > Gudrun Lang > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From jstaruk <@t> masshistology.com Tue Jul 18 17:14:31 2006 From: jstaruk <@t> masshistology.com (Jim Staruk) Date: Tue Jul 18 17:14:07 2006 Subject: [Histonet] necrosis detection In-Reply-To: <20060718202507.12459.qmail@web61222.mail.yahoo.com> Message-ID: <000301c6aab7$8b803490$6500a8c0@FrontOffice> I thinks it's a little more complicated than that! A lot goes on in-vivo once an area of tissue dies. I have a nice collection of cardiac slides of infarcts that are a few minutes old to a day old (from my days with the Medical Examiner's Office) and every slide is a little different. You can see cytoplasm membranes rupturing in the very early slides, vessel walls breaking down (causing hemorrhage) early on, neutrophil migration, there's enzyme activity, etc, etc. On the other hand, I just processed a piece of "fresh" stake purchased at the grocery store (I'm guessing the meat is at least a week dead although refrigerated) and aside from a few swollen nuclei, it looked perfectly viable. There's papers published on this, probably more in the forensic manuscripts. Jim ______________________ Jim Staruk Mass Histology Service www.masshistology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, July 18, 2006 4:25 PM To: histology@gradymem.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] necrosis detection Angie: Run a little expriment by leaving unfixed tissue during at least 8 hours. Fix as usual after half an hour intervals (16 in total) and process the tissue. Section and stain with H&E and let your pathologist look for the signs of necrosis in each slide from each time interval. If they are not evident, increase to more time in same time length intervals. I think this would be a good way to find out. Hope this will help you! Ren? J. histology@gradymem.org wrote: My pathologist has a question: After cell death occurs, what is the earlist time at which necrosis can be detected by H&E stains? Thanks, Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail Beta. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Robert.Lott <@t> bhsala.com Tue Jul 18 17:41:49 2006 From: Robert.Lott <@t> bhsala.com (Lott, Robert) Date: Tue Jul 18 17:42:12 2006 Subject: [Histonet] BAF47/INI1 Message-ID: It is my understanding that one of the primary applications for BAF47 is in separating different pediatric CNS neoplasms especially rhabdoid tumors. BAF47 identifies the "deletion" or mutation of the hSNF5/INI1 gene. (or the corresponding reduction in protein expression at the protein level in IHC terms). Therefore, malignant rhabdoid tumors, which can be confused with PNET's (medulobalstomas), Ewing's, Wilm's, sarcoma, rhabdo. etc., will stain "negatively" (not stain at all) while these others would stain positively or variably for the antibody. This works very well in our lab..... we have the BAF47 antibody from BD Transduction Labs diluted 1:200 (60 min.) with Envision+ detection (45 min.) DAB+ for 10 min. HIER is with Tris/EDTA pH 9.0, 20 min. heat/20 min. cool. The pathologists at the local Children's Hospital provided us with great control material and are very pleased with the result! See: Am J Surg Pathol. 2004 May;28(5):644-50. Am J Surg Pathol. 2004 Nov;28(11):1485-91. Robert Robert L. Lott, HTL(ASCP) Manager, Anatomic Pathology LabFirst / Trinity Medical Center 800 Montclair Road Birmingham, AL 35213 205-592-5388 phone 205-592-5646 fax robert.lott@bhsala.com ----------------------------------------- Confidentiality Notice: The information contained in this email message is privileged and confidential information and intended only for the use of the individual or entity named in the address. If you are not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this information is strictly prohibited. If you received this information in error, please notify the sender and delete this information from your computer and retain no copies of any of this information. From JWEEMS <@t> sjha.org Tue Jul 18 17:51:55 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Jul 18 17:50:16 2006 Subject: [Histonet] parafin on fabric chairs Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202960437@sjhaexc02.sjha.org> Do this after freezing the wax and removing all that will pop loose = then the reminder will absorb easily. My 2 cents again.. ..! j -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Roberta Horner Sent: Tuesday, July 18, 2006 10:55 AM To: Travis Troyer; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] parafin on fabric chairs This works on carpeting so you may want to try it. You need a brown paper bag and an iron. Put the paper on the chair and run the hot iron over the paper. The iron will melt the paraffin and the paper should absorb the paraffin. Roberta -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Travis Troyer Sent: Tuesday, July 18, 2006 10:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] parafin on fabric chairs We have some fabric chairs in our lab that have parafin on them. Does anyone know of a way to clean the wax off? Thanks Travis _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From anh2006 <@t> med.cornell.edu Tue Jul 18 19:09:28 2006 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Tue Jul 18 19:09:40 2006 Subject: [Histonet] Flt-1 and Flk-1 In-Reply-To: References: Message-ID: Hi Patti and Gudrun .... After extensive testing, I found that particular rabbit polyclonal from LabVision/Neomarkers against FLT-1 to be highly unspecific. Normally I love love love the antibodies from that company but I regret to say that I generally don't trust any data I see using that particular anti-FLT1 Ab. At 2:52 PM -0700 7/18/06, Patti Loykasek wrote: >Hi Gudrun. Our research department has worked with Flt-1 about a year ago. >For the Flt-1 (VEGF R-1) they use a rabbit polyclonal from Neomarkers with >a heat citrate pretreatment. We do not have any experience with Flk-1. >Hope this helps. > > >Patti Loykasek BS, HTL, QIHC >PhenoPath Laboratories >Seattle, WA > > > > >> Hi Histonetters, >> Flt-1 and Flk-1: Is anybody successfull with ihc-staining of these two >> antibodies (human tissue)? I use the Ventana benchmark XT (CC1 mild, 1:40, >> 40 min). >> Untill now I've got nuclear staining instead of cytoplasmic in placenta. >> Any hints are appreciated. >> >> Gudrun Lang > > > -- From m.wei <@t> biogenex.com Tue Jul 18 19:24:10 2006 From: m.wei <@t> biogenex.com (May Wei) Date: Tue Jul 18 19:24:15 2006 Subject: [Histonet] Cdx clone 88 Message-ID: <37DC9F93CF7F864182D0463EF93D571B20643E@ISLETON2.california.biogenex.com> Dear Mr. Cartun: I am forwarding my colleague's email to Ms. Loykasek for your information. Thank you very much! May Wei, M. Med. BioGenex 4600 Norris Canyon Road San Ramon, CA 94583 Tel: 1 800 421-4149 From: Philipp Novales-Li Sent: Tuesday, July 18, 2006 1:27 PM To: 'ploykasek@phenopath.com' Cc: May Wei; Elina Golder-Novoselsky Subject: BioGenex CDX-2 Antibody Dear Ms. Loykasek: Germane to your most recent enquiry about a supposed discontinuation of our CDX-2 antibody, I wish to let you know that such information is balderdash and incorrect. The CDX2-88 clone is proprietary to BioGenex and we had developed this clone in-house under an exclusive license. I encourgae you to refer to Medline for a listing of a plethora of peer-reviewed papers that have utilized the BioGenex CDX-2 Antibody. Other manufacturers also purchase the said antibody from us and they resell it under their respective trade names. We therefore encourage you to purchase directly from us so that you can avail of our customer service and technical support, as well as our competitive prices. Germane to your other concern, it is our policy to inform our stakeholders of any changes that are made in our product line. Please therefore be rest assured that we will not glibly discontinue a product without notifying our customers. It is also our policy to provide alternative clones in the event that a specific clone needs to be discontinued. We therefore encourage you to contact us directly, if you have any other concerns so that we can learn from your input. For the nonce, please do not hesitate to contact me directly, or call our Customer Service at 1-800-421-4149, if you would like to learn more about our products and services. We look forward to serve you and your comments are greatly appreciated. Sincerely yours, Philipp Novales-Li, DMedSc, PhD, DPhil (Oxford) BioGenex 4600 Norris Canyon Road San Ramon, CA 94583 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Tuesday, July 18, 2006 10:57 AM To: histonet; Patti Loykasek Subject: Re: [Histonet] Cdx clone 88 Good point Patti. Dako recently discontinued several infectious disease antibodies that I use and I didn't find out about until we tried to order more antibody after we ran out. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> Patti Loykasek 07/18/06 1:29 PM >>> Hi all. I just heard that Biogenex is discontinuing cdx-2 clone 88. Does anyone know of an alternate source for this antibody, I prefer concentrates? I do know that BioCare carries a predilute. I appreciate the help. For any vendors out there, it would be nice if you would inform your customers when you are going to discontinue a product. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------ - This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kemlo.rogerson <@t> waht.swest.nhs.uk Wed Jul 19 02:12:28 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Jul 19 02:11:50 2006 Subject: [Histonet] parafin on fabric chairs Message-ID: We aren't allowed to have fabric chairs in UK Labs; Health and Safety! I guess bugs live in the fabric and you catch something, never understood why those plastic chairs are allowed either; hot day lot of sitting...... unpleasant!!!!! Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 Life shrinks and expands in proportion to one's courage. --Anais Nin This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From kemlo.rogerson <@t> waht.swest.nhs.uk Wed Jul 19 02:15:04 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Jul 19 02:14:27 2006 Subject: [Histonet] necrosis detection Message-ID: Is it not autolysis rather than necrosis? Doesn't necrosis happen to tissue in a 'live' patient whilst autolysis affects those that aren't? Cells and tissue die at different rates, some cells 'live' for hours after death of its 'host'. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 Life shrinks and expands in proportion to one's courage. --Anais Nin This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From louise.renton <@t> gmail.com Wed Jul 19 03:20:11 2006 From: louise.renton <@t> gmail.com (louise renton) Date: Wed Jul 19 03:20:16 2006 Subject: [Histonet] recycling alcohol-South africa Message-ID: Hi, are there any south african histo labs recycling solvents? If so, what system do you have and approx what volumes do you deal with? TIA Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From Terry.Marshall <@t> rothgen.nhs.uk Wed Jul 19 06:15:43 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Wed Jul 19 06:12:34 2006 Subject: [Histonet] necrosis detection Message-ID: Rene - I'm surprised at you. Autolysis is not necrosis. Actually to answer the original question, there is no answer. You tell a cell is dead by its appearance, and I know not how you can tell a cell is dead until it looks dead. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: 18 July 2006 21:25 To: histology@gradymem.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] necrosis detection Angie: Run a little expriment by leaving unfixed tissue during at least 8 hours. Fix as usual after half an hour intervals (16 in total) and process the tissue. Section and stain with H&E and let your pathologist look for the signs of necrosis in each slide from each time interval. If they are not evident, increase to more time in same time length intervals. I think this would be a good way to find out. Hope this will help you! Ren? J. histology@gradymem.org wrote: My pathologist has a question: After cell death occurs, what is the earlist time at which necrosis can be detected by H&E stains? Thanks, Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail Beta. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From benardsolomona <@t> yahoo.com Wed Jul 19 06:23:21 2006 From: benardsolomona <@t> yahoo.com (Solomon Adebayo) Date: Wed Jul 19 06:23:25 2006 Subject: [Histonet] Leica microtome In-Reply-To: <5784D843593D874C93E9BADCB87342AB013072DA@tpiserver03.Coretech-holdings.com> Message-ID: <20060719112321.86741.qmail@web36102.mail.mud.yahoo.com> we recently overcame similar problem in our laboratory at the Pathology dept. Unilorin teaching hostpital ilorin Nigeria. It really gave us a lot of sleepless night. However the joy of getting over the problem through patient application of the knowledge of how the microtome works and other factors that makes for excellent manipulation of the microtome for good results was worth the effort. I will advice you to do the following: (1)Lubricate the machine with the lubricating fluid that came with it. (2)Make sure all the screws and knob on the block holder are tight and well orientated. (3)Re-set the angle of tilt. Cheers Benard Solomon Pathology Dept., Unilorin Teaching Hospital, Kwara State, Nigeria. www.histosearch/homepages/benardsolomon --- Charles Scouten wrote: > Usually, when we hear about such things, the answer > is the blade angle > is too shallow, or the pedestal has wobble for some > reason. Grasp the > tissue pedastal with your hand and try to wobble it. > If you can, that > is the problem. Has the blade angle changed, on > purpose or by accident? > Also try to wiggle the blade to see if it is loose, > but try to do that > without losing a hand. > > > Cordially, > Charles W. Scouten, Ph.D. > myNeuroLab.com > 5918 Evergreen Blvd. > St. Louis, MO 63134 > Ph: 314 522 0300 x 342 > FAX 314 522 0377 > cwscouten@myneurolab.com > http://www.myneurolab.com > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Dr. F. > Tryka > Sent: Friday, July 14, 2006 2:36 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Leica microtome > > We are seeking advice. Our Leica 2125 RM microtome > has been functioning > well until it suddenly started cutting thick thin > sections. > We have tried to align it, etc and it still is not > functioning properly. > > > The block face looks smooth, but it will cut several > slices toward one > side, then on the next pass it cuts off a potato > chip slice. Any > suggestions would be appreciated. Franci Tryka > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From coleman_manufacturing <@t> yahoo.com Wed Jul 19 08:16:39 2006 From: coleman_manufacturing <@t> yahoo.com (Coleman Manufacturing) Date: Wed Jul 19 08:16:44 2006 Subject: [Histonet] Fisher Scientific distributor Message-ID: <20060719131639.30635.qmail@web37603.mail.mud.yahoo.com> Hello everyone! Let me first say I hope this message does not make anyone mad, for I know HistoNet was intended for educational and information sharing purposes only. With this said, I am Justin Coleman with Coleman Manufacturing out of South Carolina. Just wanted to post a message to everyone informing all that CMD is now a direct distributor for Fisher Scientific. We distribute any and all products from Fisher. For example, we distribute laboratory equipment (Microwaves, cryostats, etc.), laboratory reagents, special stains, and disposables. Please do not hesitate to call us if we can help you out; maybe we can do better on pricing for you! Thanks to all, Justin Coleman Coleman Manufacturing & Design South Carolina 803.633.2124 office 803.635-9401 fax From gu.lang <@t> gmx.at Wed Jul 19 09:06:40 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Jul 19 09:06:49 2006 Subject: AW: [Histonet] necrosis detection In-Reply-To: Message-ID: <000301c6ab3c$8ebb12d0$eeeea8c0@SERVER01> I've allways thought, that autolysis is part of necrosis. Cell get damaged, targeted by other cells, no oxygen or any other causes. And then the cellmembrans brake and the hydrolases begin to "eat" up the cell. Isn't it like this? Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Marshall Terry Dr,Consultant Histopathologist Gesendet: Mittwoch, 19. Juli 2006 13:16 An: Rene J Buesa; histology@gradymem.org; histonet@lists.utsouthwestern.edu Betreff: RE: [Histonet] necrosis detection Rene - I'm surprised at you. Autolysis is not necrosis. Actually to answer the original question, there is no answer. You tell a cell is dead by its appearance, and I know not how you can tell a cell is dead until it looks dead. Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Rene J Buesa [mailto:rjbuesa@yahoo.com] Sent: 18 July 2006 21:25 To: histology@gradymem.org; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] necrosis detection Angie: Run a little expriment by leaving unfixed tissue during at least 8 hours. Fix as usual after half an hour intervals (16 in total) and process the tissue. Section and stain with H&E and let your pathologist look for the signs of necrosis in each slide from each time interval. If they are not evident, increase to more time in same time length intervals. I think this would be a good way to find out. Hope this will help you! Ren? J. histology@gradymem.org wrote: My pathologist has a question: After cell death occurs, what is the earlist time at which necrosis can be detected by H&E stains? Thanks, Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail Beta. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Wed Jul 19 09:17:18 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Jul 19 09:17:30 2006 Subject: [Histonet] necrosis detection Message-ID: <09C945920A6B654199F7A58A1D7D1FDE0171775E@lsexch.lsmaster.lifespan.org> Necrosis is, technically, cell death. Once a cell is dead, necrosis has occurred. Autolysis is a post-mortem process whereby dead cells are broken down by the release of their own autogenous enzymes. So, in the strict sense, necrosis precedes autolysis. However, necrosis per se cannot be directly observed. A cell that has just died is still visually identical to a living cell. What we commonly refer to as "necrosis" in a tissue section is not merely cell death, but the accumulation of postmortem changes whereby we can visually identify cells as dead. Such postmortem changes are largely the result of autolysis. So in that sense "necrosis", or more properly "necrotic change", results from, rather than precedes, autolysis. From settembr <@t> umdnj.edu Wed Jul 19 09:18:17 2006 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Wed Jul 19 09:18:58 2006 Subject: [Histonet] BAF47 (Ini1) antibody Message-ID: I, too use BAF47 from BD successfully. I use it at 1:50 for 15 minute RT. Steamer with Dako's Target Retrieval Solution and their LSAB2 detection kit. Works nicely. Seems like it will work with many detection systems. Dana Settembre University Hospital - UMDNJ Newark, NJ USA >>> Tracey Gunn 07/17/06 6:39 PM >>> Hello, Is anyone successfully using this antibody on formalin-fixed, paraffin embedded tissue? Thanks, Tracey Gunn. Tracey Gunn, Section Leader Histology, LabPlus, Auckland City Hospital, Auckland, New Zealand. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From froyer <@t> bitstream.net Wed Jul 19 09:38:56 2006 From: froyer <@t> bitstream.net (Ford Royer) Date: Wed Jul 19 09:39:37 2006 Subject: [Histonet] necrosis detection In-Reply-To: <09C945920A6B654199F7A58A1D7D1FDE0171775E@lsexch.lsmaster.lifespan.org> Message-ID: <002601c6ab41$10b466c0$7701a80a@Ford> Bingo... I think that Paul's got it. Isn't ALL tissue that you look at necrotic? (even fresh frozen sections?) ;-) ~ Ford Ford M. Royer, MT(ASCP) Minnesota Medical Minneapolis, MN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Wednesday, July 19, 2006 9:17 AM To: 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] necrosis detection Necrosis is, technically, cell death. Once a cell is dead, necrosis has occurred. Autolysis is a post-mortem process whereby dead cells are broken down by the release of their own autogenous enzymes. So, in the strict sense, necrosis precedes autolysis. However, necrosis per se cannot be directly observed. A cell that has just died is still visually identical to a living cell. What we commonly refer to as "necrosis" in a tissue section is not merely cell death, but the accumulation of postmortem changes whereby we can visually identify cells as dead. Such postmortem changes are largely the result of autolysis. So in that sense "necrosis", or more properly "necrotic change", results from, rather than precedes, autolysis. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From wellborj <@t> mercyhealth.com Wed Jul 19 09:57:06 2006 From: wellborj <@t> mercyhealth.com (Janci Wellborn) Date: Wed Jul 19 09:57:35 2006 Subject: [Histonet] necrosis detection Message-ID: IS it Friday already? >>> "Monfils, Paul" 7/19/2006 9:17 AM >>> Necrosis is, technically, cell death. Once a cell is dead, necrosis has occurred. Autolysis is a post-mortem process whereby dead cells are broken down by the release of their own autogenous enzymes. So, in the strict sense, necrosis precedes autolysis. However, necrosis per se cannot be directly observed. A cell that has just died is still visually identical to a living cell. What we commonly refer to as "necrosis" in a tissue section is not merely cell death, but the accumulation of postmortem changes whereby we can visually identify cells as dead. Such postmortem changes are largely the result of autolysis. So in that sense "necrosis", or more properly "necrotic change", results from, rather than precedes, autolysis. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ree3 <@t> leicester.ac.uk Wed Jul 19 10:04:09 2006 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Wed Jul 19 10:04:15 2006 Subject: [Histonet] necrosis detection In-Reply-To: <002601c6ab41$10b466c0$7701a80a@Ford> Message-ID: for those interested "Classification of cell death: recommendations of the Nomenclature Committee on Cell Death" Kroemer et al, Cell Death and Differentiation(2005)12, 1463-1467. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Ford Royer Sent: 19 July 2006 15:39 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] necrosis detection No I don't think so.. Bingo... I think that Paul's got it. Isn't ALL tissue that you look at necrotic? (even fresh frozen sections?) ;-) ~ Ford Ford M. Royer, MT(ASCP) Minnesota Medical Minneapolis, MN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Wednesday, July 19, 2006 9:17 AM To: 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] necrosis detection Necrosis is, technically, cell death. Once a cell is dead, necrosis has occurred. Autolysis is a post-mortem process whereby dead cells are broken down by the release of their own autogenous enzymes. So, in the strict sense, necrosis precedes autolysis. However, necrosis per se cannot be directly observed. A cell that has just died is still visually identical to a living cell. What we commonly refer to as "necrosis" in a tissue section is not merely cell death, but the accumulation of postmortem changes whereby we can visually identify cells as dead. Such postmortem changes are largely the result of autolysis. So in that sense "necrosis", or more properly "necrotic change", results from, rather than precedes, autolysis. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From David.Edmondson <@t> christie-tr.nwest.nhs.uk Wed Jul 19 11:25:42 2006 From: David.Edmondson <@t> christie-tr.nwest.nhs.uk (Edmondson David (RBV) NHS Christie Tr) Date: Wed Jul 19 11:29:48 2006 Subject: [Histonet] Leica microtome Message-ID: <3C83687E8F6AE04792E361ABE2D385B8418165@cht-mail2-2k.xchristie.nhs.uk> I remember on the Leica 1512 looseness in the up/down travel by virtue, in part, of unever wear of the surfaces. Microtomists trimming in the centre of the travel rather than performing a complete cycle of the handle. Such adjustment as was possible would find the travel tight at bottom and top of the stroke and loose in the cutting section of the travel. Fresh oil, and then even more viscous oil was the best we could do to reduce the wobble described and still have free rotation. Money was found in the end,the microtome then became a source of spare parts, then was tipped. David Manchester UK -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Solomon Adebayo Sent: 19 July 2006 12:23 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Leica microtome we recently overcame similar problem in our laboratory at the Pathology dept. Unilorin teaching hostpital ilorin Nigeria. It really gave us a lot of sleepless night. However the joy of getting over the problem through patient application of the knowledge of how the microtome works and other factors that makes for excellent manipulation of the microtome for good results was worth the effort. I will advice you to do the following: (1)Lubricate the machine with the lubricating fluid that came with it. (2)Make sure all the screws and knob on the block holder are tight and well orientated. (3)Re-set the angle of tilt. Cheers Benard Solomon Pathology Dept., Unilorin Teaching Hospital, Kwara State, Nigeria. www.histosearch/homepages/benardsolomon --- Charles Scouten wrote: > Usually, when we hear about such things, the answer is the blade angle > is too shallow, or the pedestal has wobble for some reason. Grasp the > tissue pedastal with your hand and try to wobble it. > If you can, that > is the problem. Has the blade angle changed, on purpose or by > accident? > Also try to wiggle the blade to see if it is loose, but try to do that > without losing a hand. > > > Cordially, > Charles W. Scouten, Ph.D. > myNeuroLab.com > 5918 Evergreen Blvd. > St. Louis, MO 63134 > Ph: 314 522 0300 x 342 > FAX 314 522 0377 > cwscouten@myneurolab.com > http://www.myneurolab.com > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] > On Behalf Of Dr. F. > Tryka > Sent: Friday, July 14, 2006 2:36 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Leica microtome > > We are seeking advice. Our Leica 2125 RM microtome has been > functioning well until it suddenly started cutting thick thin > sections. > We have tried to align it, etc and it still is not functioning > properly. > > > The block face looks smooth, but it will cut several slices toward one > side, then on the next pass it cuts off a potato chip slice. Any > suggestions would be appreciated. Franci Tryka > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************** This e-mail and any files transmitted with it are confidential and solely for the use of the intended recipient. If you have received this e-mail in error you should not disseminate, distribute or copy it. Please notify the sender immediately and delete this e-mail from your system. ******************************************************************************************************************* From ccrowder <@t> vetmed.lsu.edu Mon Jul 17 17:13:39 2006 From: ccrowder <@t> vetmed.lsu.edu (Cheryl Crowder) Date: Wed Jul 19 12:24:06 2006 Subject: [Histonet] Problems with eyes Message-ID: Hello - I am working with whole eye retinas. The retinas are collected, defatted, rehydrated to distilled water, stained, dehydrated through graded alcohols and cleared in xylene. Here's the problem: When the tissues hit the xylene they disintegrate! Not wash off - I mean disintegrate. Have any of you heard of this problem before and, if so, what did you do about it? I would appreciate any comments, thoughts or help with this problem. Maybe one of you who works exclusively with eyes has seen this before. Thank you for your help, Cheryl Cheryl Crowder, BA, HTL(ASCP) Chief Technologist Anatomic Pathology Department of Pathobiological Sciences School of Veterinary Medicine Louisiana State University Skip Bertman Drive Baton Rouge, LA 70803 225-578-9734 FAX: 225-578-9720 From LiebenbergE <@t> orthosurg.ucsf.edu Mon Jul 17 13:18:26 2006 From: LiebenbergE <@t> orthosurg.ucsf.edu (Liebenberg, Ellen) Date: Wed Jul 19 12:24:08 2006 Subject: [Histonet] RE: Tissue loss In-Reply-To: Message-ID: <60E0B5C8212B9040A390B7EE167E1181B858B2@SOMMAIL.som.ucsf.edu> We had the same problem with tissue coming of the slide after heat treatment. Bone is particularly prone to loosening. For our specimens the fix was as follows: 1. After sectioning, dry the slides at 37 degrees overnight, then melt the slides at 60 degrees for 30 minutes in a slide rack so they are vertical (the paraffin should drip down the slide), and cool prior to deparaffinization. 2. After the second deparaffinization (we use Clearite), air dry the slides about 15 minutes, or until all the sections are completely free of Clearite. They should look white. Then go into the third deparaffinzation for the normal time. We do 10 min per change. Believe it or not, drying during the deparafinnization steps does not impair immunostaining. These suggestions came from some now frequent Histonet contributors in response to my desperate phone calls back in the pre-Histonet days. Hope this helps. Ellen Liebenberg University of California Medical Center San Francisco, Ca 415-502-0809 From rachael_emerson <@t> urmc.rochester.edu Wed Jul 19 12:49:53 2006 From: rachael_emerson <@t> urmc.rochester.edu (Rachael Emerson) Date: Wed Jul 19 12:51:13 2006 Subject: [Histonet] Fc epsilon receptor Message-ID: Hello. Does anyone work with a Fc epsilon receptor antibody that they use for immunos, either on frozens or parafin? I am looking for a place to buy this antibody. Thanks Rachael Emerson -- Rachael L. Emerson Center for Pediatric Biomedical Research University of Rochester Medical Center 575 Elmwood Avenue MRBX 1.11301 Rochester, NY 14642 From PMonfils <@t> Lifespan.org Wed Jul 19 13:13:47 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Jul 19 13:13:53 2006 Subject: [Histonet] Problems with eyes Message-ID: <09C945920A6B654199F7A58A1D7D1FDE0171775F@lsexch.lsmaster.lifespan.org> Cheryl, You didn't mention fixation. Are the eyes fixed before the retinas are collected? If so, what is the fixation protocol? From ploykasek <@t> phenopath.com Wed Jul 19 13:44:33 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Wed Jul 19 13:44:48 2006 Subject: [Histonet] Cdx Message-ID: Hi all. I just wanted to let everyone know that I found out Biogenex is continuing to carry the antibody to CDX2 clone 88. This clone is proprietary to Biogenex. Hope I didn't panic too many colleagues. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From PKamalavenkatesh <@t> wockhardtin.com Thu Jul 20 01:01:40 2006 From: PKamalavenkatesh <@t> wockhardtin.com (PKamalavenkatesh@wockhardtin.com) Date: Thu Jul 20 00:59:04 2006 Subject: [Histonet] reply: Ncrosis detection Message-ID: Necrosis is currently used to indicate non apoptotic accidental form of cell death. But please understand that cell death and necrosis are two different entities. For example if rat liver cells are subjected to ischemia, hydrolysis and denaturation of cellular proteins started at 30 minutes and cells reached the point of no return (this is actually the cell death) at 2.0-2.5 hrs. This point of no return is characterized by massive caspase activation, loss of mitochondrial transmembrane potential and exposure of phosphatidylserine groups, which make the cells to be identified by the phagocytic cells, and aids in phagocytosis (I can tell EAT ME signal). Now histological identification will come only at 12-24 hrs. That means cells die long before any necrotic changes seen under light microscope. So cell death and necrosis are two different entities and should not be used as synonyms. If you have any queries, please get back to me. I have some superb references dealing with this always controversial topic. REGARDS Dr.P.Kamalavenkatesh Discovery- Biology Pre clinical Safety Assessment Wockhardt Research Center, Aurangabad, India. E.mail : Pkamalavenkatesh@wockhardtin.com Please Visit our New Corporate Web Site www.wockhardt.com --------------------- Disclaimer ------------------------------------------------------------------------------ Information transmitted by this E-MAIL is proprietary to Wockhardt Ltd. and/ or its Group Companies and/or,its Customers and is intended for use only by the individual or entity to which it is addressed, and may contain information that is privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. --------------------------------------------------------------------------------------------------------------- From kemlo.rogerson <@t> waht.swest.nhs.uk Thu Jul 20 02:27:11 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Thu Jul 20 02:26:35 2006 Subject: [Histonet] necrosis detection Message-ID: Something to do with the sodium and potassium pumps stopping but that's much too complicated for me; I suppose it's dead when it starts to smell? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 Life shrinks and expands in proportion to one's courage. --Anais Nin This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From cmalc <@t> unimelb.edu.au Thu Jul 20 03:40:05 2006 From: cmalc <@t> unimelb.edu.au (Cathy Malcontenti-Wilson) Date: Thu Jul 20 03:40:22 2006 Subject: [Histonet] Growth factor receptor antibodies Message-ID: <6.2.1.2.2.20060720183703.02cf69c8@mail.staff.unimelb.edu.au> Dear Histonetters, Does anyone know of any good antibodies which work for immunohistochemistry on formalin fixed mouse tissue embedded in paraffin for the following 3 growth factor receptors?: (1) Insulin-like Growth Factor 1 Receptor (2) Epidermal Growth Factor Receptor (3) Hepatocyte Growth Factor Receptor aka c-MET Thanks very much for your help. Cathy Malcontenti-Wilson From rasha_kanhoush <@t> yahoo.com Thu Jul 20 05:07:54 2006 From: rasha_kanhoush <@t> yahoo.com (rasha kanhoush) Date: Thu Jul 20 05:07:59 2006 Subject: [Histonet] manual microtome 5030 Message-ID: <20060720100754.2686.qmail@web53605.mail.yahoo.com> we lost our manual for the microtome 5030 ( Bright instrument). would you have a copy of this manual in your drawers? it's an emergency, so I would really appreciate it if somone can semd me a PDF format of the manual. Thanks. rasha kanhoush --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail Beta. From abright <@t> brightinstruments.com Thu Jul 20 05:59:26 2006 From: abright <@t> brightinstruments.com (Alan Bright) Date: Thu Jul 20 05:47:45 2006 Subject: [Histonet] manual microtome 5030 Message-ID: Dear Rasha, Please could you let me have the serial number for this old Bright 5030 microtome also your Fax number as this model is pre PDF's. For fast assistance you could contact a USA distributor or alternatively call me on one of the numbers below. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype User ID: dazzle0 Windows Live Messenger User ID: dazzlethe1@hotmail.com -----Original Message----- From: rasha kanhoush [mailto:rasha_kanhoush@yahoo.com] Sent: 20 July 2006 11:08 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] manual microtome 5030 we lost our manual for the microtome 5030 ( Bright instrument). would you have a copy of this manual in your drawers? it's an emergency, so I would really appreciate it if somone can semd me a PDF format of the manual. Thanks. rasha kanhoush --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail Beta. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Thu Jul 20 11:39:04 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Jul 20 11:39:13 2006 Subject: [Histonet] Request for a copy of pages on GFP visualization in FFPE tissue Message-ID: <6.0.0.22.1.20060720103224.01bae008@gemini.msu.montana.edu> Dear all, If anyone has this manual, could you send a copy of Page 696 on protocol for fixation and paraffin embedding for GFP visualization. Manipulating the Mouse Embryo: A Laboratory Manual, 3rd ed. Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer. 2003. Cold Spring Harbor Laboratory Press. ISBN 0-87969-574-9 page 696 for protocol on fixation and paraffin embedding for GFP visualization. We do not have the manual in our laboratory and are doing some outside processing for other people. Also, I would entertain any comments on success or lack of success on this subject. I has been discussed before, but methods improve over the years on maintaining eGFP fluorescence after FFPE. I would appreciate detailed processing schedules, in particular, use of vacuum/pressures, times in solvents, and paraffin temperatures. You can send it privately if you wish. Thanks in advance Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From Sue.Tyler <@t> noaa.gov Thu Jul 20 12:49:05 2006 From: Sue.Tyler <@t> noaa.gov (Sue Tyler) Date: Thu Jul 20 12:49:14 2006 Subject: [Histonet] more on air filtration system Message-ID: <44BFC211.4040006@noaa.gov> For those of you who use the Market Lab air filtration system, better known as R2D2, do you have any problems with the disposal of the old filters? Apparently there are some problems with how to dispose of it as well as disposal costs. Any suggestions? Sue Cooperative Oxford Lab 904 S. Morris Street Oxford, Maryland 21654 From olivamor <@t> post.tau.ac.il Thu Jul 20 13:08:53 2006 From: olivamor <@t> post.tau.ac.il (Moran Oliva) Date: Thu Jul 20 13:09:06 2006 Subject: [Histonet] confocal condition for Cy2 and Cy3 IHC on plants Message-ID: <003201c6ac27$902d0790$0100000a@moran> Hello all! I've just joined this forum. It is very useful. I've started a preliminary assay of Immunohistochemistry on Arabidopsis tissue (Steedman's protocol) using my GFP-protein transgenic plants as a positive control (to confirm the method is working for me). The anti-GFP antibodies I used were either from mouse or from rabbit. So when I used the Cy2 goat anti-mouse I needed to use the same emission spectra as for the GFP protein itself- Does anyone knows whether the GFP survive all this process? does anyone has any good advise of detecting the IHC signal and not theprotein GFP signal? the second channel I used was GFP autoflouresence... Does anyone know what are the confocal setting details for Cy3 in plant tissue? I've tried to detect the GFP in IHC using rabbit GFP protein, so my second antibody is Cy3 conjugated ( I know the excitation and emission spectra, but the autoflourescence is very close to its wave lengths-do I need to use a second channel for autoflourescence in this case too? Thank you very much looking forward for your advices Moran From Margaret.Perry <@t> sdstate.edu Thu Jul 20 13:20:50 2006 From: Margaret.Perry <@t> sdstate.edu (Perry, Margaret) Date: Thu Jul 20 13:21:00 2006 Subject: [Histonet] coxiella burnetti Message-ID: Does anyone know of a lab that does PCR on FFPE for Coxiella burnetti? I would also be interested in any other test for Coxiella on FFPE other than IHC. Margaret Perry South Dakota State University Brookings SD From la.sebree <@t> hosp.wisc.edu Thu Jul 20 13:22:11 2006 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Thu Jul 20 13:22:20 2006 Subject: [Histonet] alpha/beta lymphocyte and delta/gamma lymphocyte immunostaining Message-ID: Hi Histonetters, One of our pathologists wants us to find out if there are antibodies/reference labs out there that stain for these 2 antigens. We know it can be done by flow cytometry but he wants it done on paraffin sections. Thanks, Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From bgrunewald <@t> ucdavis.edu Thu Jul 20 13:25:39 2006 From: bgrunewald <@t> ucdavis.edu (Rebecca Grunewald) Date: Thu Jul 20 13:25:47 2006 Subject: [Histonet] obtaining ethopropazine? Message-ID: <200607201825.k6KIPdBe003206@lymeon.ucdavis.edu> We are trying to do an old acetylcholinesterase stain that calls for ethopropazine, but I have discovered that it is now used as an anti- parkinson's drug and is available only through prescription. Has anyone else encountered this problem and what did you do to obtain it? It is now prescribed under the brandname parsidol. From Jodiputnam <@t> aol.com Thu Jul 20 13:44:10 2006 From: Jodiputnam <@t> aol.com (Jodiputnam@aol.com) Date: Thu Jul 20 13:44:22 2006 Subject: [Histonet] Immunofluorescence question Message-ID: <322.83d8307.31f128fa@aol.com> Hi. Just have a few questions for those of you that do immunofluorescence. Typically I do mostly skin punches and shaves for our dermatopath. My question is, do any of you order the PBS that is used for the procedure or make it as needed? I was making it up as needed, but there has been an increase in the cases and I wondered if anyone ordered commercially. If so, please let me know a good vendor and I will get some ordered. Nothing worse that starting to process your specimen just to notice that someone left you an empty bottle of PBS. I've only been doing this procedure for a few months and I am always looking for little tips to improve my technique. So far things have been going well. I had a problem with tissue washing off before applying the antibodies but seem to have improved that area. I have tried applying heat, extending drying times etc., but inevitably there is still some tissue loss. I put several sections on each slide with the control and seem to lose usually 1 section of the patient tissue. I would love to have all sections stay until the coverslipping stage but maybe that's my OCD and not realistic. Thanks for any info or words of wisdom you can share with me. Jodi L. Putnam HT (ASCP) St. Thomas Hospital Nashville, Tennessee 615-389-9930 From Erin.Wrona <@t> kp.org Thu Jul 20 13:48:42 2006 From: Erin.Wrona <@t> kp.org (Erin.Wrona@kp.org) Date: Thu Jul 20 13:49:13 2006 Subject: [Histonet] Asst Manager position at Kaiser San Francisco Message-ID: Hi Everyone, There is an assistant manager posistion open at Kaiser Permanente, San Francisco. Don't be intimidated by the qualifiacations listed - the management team and chief pathologist have said that they will train the appropriate applicant in pathology, immuno, CAP, CLIA, OSHA, CAstate, title 17, title 22, instrumentation, LIS, working in a union environment and any other issues. Even those in research who have never worked in a hospital lab or have no high volume experience should consider applying. The posting is on the Kaiser website, www.kp.org, and it is job ID # SF.0600369 -Erin CONFIDENTIAL OR PRIVILEGED: This communication contains information intended only for the use of the individuals to whom it is addressed and may contain information that is privileged, confidential or exempt from other disclosure under applicable law. If you are not the intended recipient, you are notified that any disclosure, printing, copying, distribution or use of the contents is prohibited. If you have received this in error, please notify the sender immediately by telephone or by returning it by reply email and then permanently deleting the communication from your system. Thank you. From nyilmaz <@t> mersin.edu.tr Thu Jul 20 22:41:53 2006 From: nyilmaz <@t> mersin.edu.tr (nyilmaz@mersin.edu.tr) Date: Thu Jul 20 14:40:13 2006 Subject: [Histonet] Sperm staining Message-ID: <20060720194153.4602E4591A@mail.mersin.edu.tr> Dear Colleagues We are staining sperm smears for evaluating male factor infertility in our lab. We use Spermac or difquik usually. Does anybody know what this commercial stains contains (including fixative solution) ? Because we somtimes have some problems find these stains. Thanks in advance Dr. Necat Yilmaz From jmyers1 <@t> aol.com Thu Jul 20 15:03:06 2006 From: jmyers1 <@t> aol.com (jmyers1@aol.com) Date: Thu Jul 20 15:03:15 2006 Subject: [Histonet] Guidelines for validating IHC procedures Message-ID: <8C87A4EA69DBE78-E30-5ED0@MBLK-M33.sysops.aol.com> Fellow HistoNetters: Does anyone know where I might be able to find written guidelines for 'validating' IHC stains? By this I mean, are there specific rules that one should follow, which would not only address our scientific desire to ensure that a procedure is working correctly, but would also satisfy various accrediting agency standards? Any feedback would be greatly appreciated... Thanks, Joe Myers ________________________________________________________________________ Check out AOL.com today. Breaking news, video search, pictures, email and IM. All on demand. Always Free. From kellye <@t> KGH.KARI.NET Thu Jul 20 15:13:35 2006 From: kellye <@t> KGH.KARI.NET (Kelly, Elisa) Date: Thu Jul 20 15:13:43 2006 Subject: [Histonet] Geimsa stain Message-ID: Hello This is my first time using this list server and I hope it works. Does anyone use the modified Diff Quik Geimsa stain for Helicobacter Pylori ? I would like to order a kit to trial but I can't find a company that sells it. Any info would be appreciated. Elisa Kelly Kingston General Hospital Kingston, Ontario Canada From MSHERWOOD <@t> PARTNERS.ORG Thu Jul 20 15:17:06 2006 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Thu Jul 20 15:17:14 2006 Subject: [Histonet] Linscott's Directory Message-ID: <1AF23D0AD12E7444A5DB083CA978B73407A30A9D@PHSXMB1.partners.org> To all: I have noticed a lot of inquiries re: specific antibodies and where to order them. There is a wonderful directory (available on-line only now) that lists antibodies and where to order them. Please see: www.linscott'sdirectory.com Should answer many, if not all, of the questions posted. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) msherwood@partners.org From MSHERWOOD <@t> PARTNERS.ORG Thu Jul 20 15:23:31 2006 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Thu Jul 20 15:23:37 2006 Subject: [Histonet] FW: Linscott's Directory(revised message) Message-ID: <1AF23D0AD12E7444A5DB083CA978B73407A30A9E@PHSXMB1.partners.org> I just tried clicking on the website listed below and I get the Microscopy ListServer! Just google Linscott's Directory if you have trouble accessing it. Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) msherwood@partners.org -----Original Message----- From: Sherwood, Margaret Sent: Thursday, July 20, 2006 4:17 PM To: 'histonet@lists.utsouthwestern.edu' Subject: Linscott's Directory To all: I have noticed a lot of inquiries re: specific antibodies and where to order them. There is a wonderful directory (available on-line only now) that lists antibodies and where to order them. Please see: www.linscott'sdirectory.com Should answer many, if not all, of the questions posted. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) msherwood@partners.org From LuckG <@t> empirehealth.org Thu Jul 20 15:36:16 2006 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Thu Jul 20 15:36:20 2006 Subject: [Histonet] FW: Linscott's Directory(revised message) Message-ID: <6BB8BC4519AAB844B174FC739A679BBC1C316B@IRMEXCH01.irm.inhs.org> Hello, "Abcam" is also a wonderful directory for Ab's. Greg -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Thursday, July 20, 2006 1:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Linscott's Directory(revised message) I just tried clicking on the website listed below and I get the Microscopy ListServer! Just google Linscott's Directory if you have trouble accessing it. Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) msherwood@partners.org -----Original Message----- From: Sherwood, Margaret Sent: Thursday, July 20, 2006 4:17 PM To: 'histonet@lists.utsouthwestern.edu' Subject: Linscott's Directory To all: I have noticed a lot of inquiries re: specific antibodies and where to order them. There is a wonderful directory (available on-line only now) that lists antibodies and where to order them. Please see: www.linscott'sdirectory.com Should answer many, if not all, of the questions posted. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) msherwood@partners.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lao_ji <@t> yahoo.com Thu Jul 20 15:38:03 2006 From: lao_ji <@t> yahoo.com (Jimmy Lao) Date: Thu Jul 20 15:38:09 2006 Subject: [Histonet] Immunofluorescence question In-Reply-To: <322.83d8307.31f128fa@aol.com> Message-ID: <20060720203803.75585.qmail@web30703.mail.mud.yahoo.com> Jodi, We are doing Immunofluorescence on mouse tissue for research, The current commercial PBS we use is from Mediatech, Inci n Herndon, VA 20171 Cat# 21-031-CM, they call that DPBS. Hope this help!. Jimmy --- Jodiputnam@aol.com wrote: > Hi. Just have a few questions for those of you that > do immunofluorescence. > Typically I do mostly skin punches and shaves for > our dermatopath. My question > is, do any of you order the PBS that is used for the > procedure or make it as > needed? I was making it up as needed, but there has > been an increase in the > cases and I wondered if anyone ordered commercially. > If so, please let me > know a good vendor and I will get some ordered. > Nothing worse that starting to > process your specimen just to notice that someone > left you an empty bottle of > PBS. I've only been doing this procedure for a few > months and I am always > looking for little tips to improve my technique. So > far things have been going > well. I had a problem with tissue washing off before > applying the antibodies > but seem to have improved that area. I have tried > applying heat, extending > drying times etc., but inevitably there is still > some tissue loss. I put several > sections on each slide with the control and seem to > lose usually 1 section of > the patient tissue. I would love to have all > sections stay until the > coverslipping stage but maybe that's my OCD and not > realistic. > > Thanks for any info or words of wisdom you can share > with me. > > > > Jodi L. Putnam > HT (ASCP) > St. Thomas Hospital > Nashville, Tennessee > 615-389-9930 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From lao_ji <@t> yahoo.com Thu Jul 20 15:56:29 2006 From: lao_ji <@t> yahoo.com (Jimmy Lao) Date: Thu Jul 20 15:56:34 2006 Subject: [Histonet] Request for a copy of pages on GFP visualization in FFPE tissue In-Reply-To: <6.0.0.22.1.20060720103224.01bae008@gemini.msu.montana.edu> Message-ID: <20060720205629.23150.qmail@web30711.mail.mud.yahoo.com> Gayle, I sent you the copy. Hope this help! Jimmy --- Gayle Callis wrote: > Dear all, > > If anyone has this manual, could you send a copy of > Page 696 on protocol > for fixation and paraffin embedding for GFP > visualization. > > Manipulating the Mouse Embryo: A Laboratory > Manual, 3rd ed. > Andras Nagy, Marina Gertsenstein, Kristina > Vintersten, and Richard > Behringer. 2003. > Cold Spring Harbor Laboratory Press. > ISBN 0-87969-574-9 > page 696 for protocol on fixation and paraffin > embedding for GFP visualization. > > We do not have the manual in our laboratory and are > doing some outside > processing for other people. > > Also, I would entertain any comments on success or > lack of success on this > subject. I has been discussed before, but methods > improve over the years > on maintaining eGFP fluorescence after FFPE. I > would appreciate detailed > processing schedules, in particular, use of > vacuum/pressures, times in > solvents, and paraffin temperatures. You can send > it privately if you wish. > > Thanks in advance > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 > 406 994-4303 (FAX) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From DEllenburg2 <@t> stfrancishealth.org Thu Jul 20 16:16:22 2006 From: DEllenburg2 <@t> stfrancishealth.org (DEllenburg2@stfrancishealth.org) Date: Thu Jul 20 16:15:37 2006 Subject: [Histonet] Jop Opening Message-ID: <0A502E8156DAA4468CB8979B27177555016CC7D2@BSSMSX5501> Bon Secours St. Francis Health System in Greenville SC has an opening for a fulltime HT or HT eligible. If interested please contact Debbie Ellenburg at 864-255-1582 or at 864-255-1278. An application can be filled out online at www.stfrancishealth.org . . The information in this communication is intended to be confidential to the Individual(s) and/or Entity to whom it is addressed. It may contain information of a Privileged and/or Confidential nature, which is subject to Federal and/or State privacy regulations. In the event that you are not the intended recipient or the agent of the intended recipient, do not copy or use the information contained within this communication, or allow it to be read, copied or utilized in any manner, by any other person(s). Should this communication be received in error, please notify the sender immediately either by response e-mail or by phone at (864) 255-1000, and permanently delete the original e-mail, attachment(s), and any copies. From JMacDonald <@t> mtsac.edu Thu Jul 20 16:30:17 2006 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Thu Jul 20 16:30:36 2006 Subject: [Histonet] Guidelines for validating IHC procedures In-Reply-To: <8C87A4EA69DBE78-E30-5ED0@MBLK-M33.sysops.aol.com> Message-ID: Cell Marque has great information on IHC validation in their catalog. Jennifer jmyers1@aol.com Sent by: histonet-bounces@lists.utsouthwestern.edu 07/20/2006 01:03 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Guidelines for validating IHC procedures Fellow HistoNetters: Does anyone know where I might be able to find written guidelines for 'validating' IHC stains? By this I mean, are there specific rules that one should follow, which would not only address our scientific desire to ensure that a procedure is working correctly, but would also satisfy various accrediting agency standards? Any feedback would be greatly appreciated... Thanks, Joe Myers ________________________________________________________________________ Check out AOL.com today. Breaking news, video search, pictures, email and IM. All on demand. Always Free. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Thu Jul 20 17:11:48 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Jul 20 17:12:00 2006 Subject: [Histonet] Immunofluorescence question In-Reply-To: <322.83d8307.31f128fa@aol.com> References: <322.83d8307.31f128fa@aol.com> Message-ID: <6.0.0.22.1.20060720155946.01b5a8d0@gemini.msu.montana.edu> We purchase our Dulbeccos PBS in bulk, a dry powder that comes in quantity to make 50 liters, goes into solution rapidly, from Sigma. Do not buy the DPBS with Mg or Ca added. Weigh out 10 grams into 1 liter of distilled water, pH is 7.4, works like a charm and very economical. You can make up to 5 or 10 liters at a time, so you have a slightly greater volume of buffer sitting around, it is stable. Or keep a 10X solution on hand so you can dilute it when needed, volume to volume, much faster than weighing and waiting. How do you fix your FS for IHC work? Time in fixative? Put your just cut FS in front of a small fan (you can try different times, i.e. 15 min versus 30 min, and use PLUS CHARGE slides, to improve drying. Losing a needed section is certainly frustrating. Heat application may destroy antigenic epitopes. At 12:44 PM 7/20/2006, you wrote: >Hi. Just have a few questions for those of you that do immunofluorescence. >Typically I do mostly skin punches and shaves for our dermatopath. >My question >is, do any of you order the PBS that is used for the procedure or make it as >needed? I was making it up as needed, but there has been an increase in the >cases and I wondered if anyone ordered commercially. If so, please let me >know a good vendor and I will get some ordered. Nothing worse that >starting to >process your specimen just to notice that someone left you an empty bottle >of >PBS. I've only been doing this procedure for a few months and I am always >looking for little tips to improve my technique. So far things have been >going >well. I had a problem with tissue washing off before applying the antibodies >but seem to have improved that area. I have tried applying heat, extending >drying times etc., but inevitably there is still some tissue loss. I put >several >sections on each slide with the control and seem to lose usually 1 section of > the patient tissue. I would love to have all sections stay until the >coverslipping stage but maybe that's my OCD and not realistic. > >Thanks for any info or words of wisdom you can share with me. > > > >Jodi L. Putnam >HT (ASCP) >St. Thomas Hospital >Nashville, Tennessee >615-389-9930 >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From vanann702 <@t> skmc.gov.ae Fri Jul 21 04:02:58 2006 From: vanann702 <@t> skmc.gov.ae (Anne Van Binsbergen) Date: Fri Jul 21 03:59:12 2006 Subject: [Histonet] Geimsa stain References: Message-ID: We use the set from (Thermo)Shandon for cyto FNAs and touch preps For all our HPs we use solution3 of the kit (blue) for 1 min, wash in water, DC&M - works like a charm! I think Shandon sell the solutions separately - check on their website Annieinarabia ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Kelly, Elisa Sent: Fri 2006/07/21 12:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Geimsa stain Hello This is my first time using this list server and I hope it works. Does anyone use the modified Diff Quik Geimsa stain for Helicobacter Pylori ? I would like to order a kit to trial but I can't find a company that sells it. Any info would be appreciated. Elisa Kelly Kingston General Hospital Kingston, Ontario Canada _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mauger <@t> email.chop.edu Fri Jul 21 06:33:53 2006 From: mauger <@t> email.chop.edu (Joanne Mauger) Date: Fri Jul 21 06:59:23 2006 Subject: [Histonet] Immunofluorescence question Message-ID: Helllo All, Do you find it necessary to use PBS vs TBS for fluorescence? My co worker recently switched from Dako TBS (addidng tween) to Labvision TBS which has tween in it. We thought these 2 TBS's would be equal, but her fluorescence did not work well, and there was lots of background. We switched her to PBS and all is well. What I'm wondering- is there something different in the Labvision TBS that could effect our regular Immunos? So far we don't see any problems, and maybe hers was a fluke. Any thoughts? Thanks, Jo >>> Gayle Callis 07/20/06 6:11 PM >>> We purchase our Dulbeccos PBS in bulk, a dry powder that comes in quantity to make 50 liters, goes into solution rapidly, from Sigma. Do not buy the DPBS with Mg or Ca added. Weigh out 10 grams into 1 liter of distilled water, pH is 7.4, works like a charm and very economical. You can make up to 5 or 10 liters at a time, so you have a slightly greater volume of buffer sitting around, it is stable. Or keep a 10X solution on hand so you can dilute it when needed, volume to volume, much faster than weighing and waiting. How do you fix your FS for IHC work? Time in fixative? Put your just cut FS in front of a small fan (you can try different times, i.e. 15 min versus 30 min, and use PLUS CHARGE slides, to improve drying. Losing a needed section is certainly frustrating. Heat application may destroy antigenic epitopes. At 12:44 PM 7/20/2006, you wrote: >Hi. Just have a few questions for those of you that do immunofluorescence. >Typically I do mostly skin punches and shaves for our dermatopath. >My question >is, do any of you order the PBS that is used for the procedure or make it as >needed? I was making it up as needed, but there has been an increase in the >cases and I wondered if anyone ordered commercially. If so, please let me >know a good vendor and I will get some ordered. Nothing worse that >starting to >process your specimen just to notice that someone left you an empty bottle >of >PBS. I've only been doing this procedure for a few months and I am always >looking for little tips to improve my technique. So far things have been >going >well. I had a problem with tissue washing off before applying the antibodies >but seem to have improved that area. I have tried applying heat, extending >drying times etc., but inevitably there is still some tissue loss. I put >several >sections on each slide with the control and seem to lose usually 1 section of > the patient tissue. I would love to have all sections stay until the >coverslipping stage but maybe that's my OCD and not realistic. > >Thanks for any info or words of wisdom you can share with me. > > > >Jodi L. Putnam >HT (ASCP) >St. Thomas Hospital >Nashville, Tennessee >615-389-9930 >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lao_ji <@t> yahoo.com Fri Jul 21 09:26:31 2006 From: lao_ji <@t> yahoo.com (Jimmy Lao) Date: Fri Jul 21 09:26:37 2006 Subject: [Histonet] Immunofluorescence question In-Reply-To: Message-ID: <20060721142631.72861.qmail@web30701.mail.mud.yahoo.com> We use PBS powder too, making 10x by kitchin, when we do Immunofluorescence, we add Triton to 0.25% please visit this website for protocol: http://saturn.med.nyu.edu/research/dg/joynerlab/protocols.html On my hand, the protocol works well on a-GFP, but background on a-Bgal, the worse the younger mouse embryos like E9.5, and I have a feeling that the 2nd Ab step in 4 C overnight looks better. I do want to see some suggestions. Thanks! Jimmy --- Joanne Mauger wrote: > Helllo All, > > Do you find it necessary to use PBS vs TBS for > fluorescence? My co > worker recently switched from Dako TBS (addidng > tween) to Labvision TBS > which has tween in it. We thought these 2 TBS's > would be equal, but her > fluorescence did not work well, and there was lots > of background. We > switched her to PBS and all is well. > What I'm wondering- is there something different in > the Labvision TBS > that could effect our regular Immunos? So far we > don't see any problems, > and maybe hers was a fluke. > Any thoughts? > > Thanks, > Jo > > >>> Gayle Callis 07/20/06 6:11 > PM >>> > We purchase our Dulbeccos PBS in bulk, a dry powder > that comes in > quantity > to make 50 liters, goes into solution rapidly, from > Sigma. Do not buy > the > DPBS with Mg or Ca added. Weigh out 10 grams into > 1 liter of > distilled > water, pH is 7.4, works like a charm and very > economical. You can > make up > to 5 or 10 liters at a time, so you have a slightly > greater volume of > buffer sitting around, it is stable. Or keep a 10X > solution on hand so > you > can dilute it when needed, volume to volume, much > faster than weighing > and > waiting. > > How do you fix your FS for IHC work? Time in > fixative? Put your just > cut > FS in front of a small fan (you can try different > times, i.e. 15 min > versus > 30 min, and use PLUS CHARGE slides, to improve > drying. Losing a needed > > section is certainly frustrating. Heat application > may destroy > antigenic > epitopes. > > > > At 12:44 PM 7/20/2006, you wrote: > >Hi. Just have a few questions for those of you that > do > immunofluorescence. > >Typically I do mostly skin punches and shaves for > our dermatopath. > >My question > >is, do any of you order the PBS that is used for > the procedure or make > it as > >needed? I was making it up as needed, but there has > been an increase > in the > >cases and I wondered if anyone ordered > commercially. If so, please let > me > >know a good vendor and I will get some ordered. > Nothing worse that > >starting to > >process your specimen just to notice that someone > left you an empty > bottle > >of > >PBS. I've only been doing this procedure for a few > months and I am > always > >looking for little tips to improve my technique. So > far things have > been > >going > >well. I had a problem with tissue washing off > before applying the > antibodies > >but seem to have improved that area. I have tried > applying heat, > extending > >drying times etc., but inevitably there is still > some tissue loss. I > put > >several > >sections on each slide with the control and seem to > lose usually 1 > section of > > the patient tissue. I would love to have all > sections stay until > the > >coverslipping stage but maybe that's my OCD and not > realistic. > > > >Thanks for any info or words of wisdom you can > share with me. > > > > > > > >Jodi L. Putnam > >HT (ASCP) > >St. Thomas Hospital > >Nashville, Tennessee > >615-389-9930 > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 > 406 994-4303 (FAX) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From gcallis <@t> montana.edu Fri Jul 21 09:28:29 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Jul 21 09:28:38 2006 Subject: [Histonet] Immunofluorescence question In-Reply-To: References: Message-ID: <6.0.0.22.1.20060721082105.01b2ffc0@gemini.msu.montana.edu> We use Dulbeccos PBS and TBS interchangeably and with a small amount of Tween only just for sheeting action over sections. We make up our own TBS, a 10X solution, and dilute it when needed to make a 0.05M TBS, at final pH of pH 7.6. Have you checked the pH of your buffer to make sure there is not some change there? Check the MSDS, product sheet or call Lab Vision tech services to discuss their buffer. I would bet their TBS is a standard recipe for making it up, and works well. For IFA work, we use 0.025% Tween 20 and for IHC 0.05% Tween 20. We never lose fluorescence (IFA) nor staining with enzyme immunohistochemistry with either buffer, and you may want to sleuth for another reason why the fluorescence was not optimal. Background generally is not caused by buffer problems, but another reason, cross reaction, too high antibody concentration, fixation issues. At 05:33 AM 7/21/2006, you wrote: >Helllo All, > >Do you find it necessary to use PBS vs TBS for fluorescence? My co >worker recently switched from Dako TBS (addidng tween) to Labvision TBS >which has tween in it. We thought these 2 TBS's would be equal, but her >fluorescence did not work well, and there was lots of background. We >switched her to PBS and all is well. >What I'm wondering- is there something different in the Labvision TBS >that could effect our regular Immunos? So far we don't see any problems, >and maybe hers was a fluke. >Any thoughts? > >Thanks, >Jo > > >>> Gayle Callis 07/20/06 6:11 PM >>> >We purchase our Dulbeccos PBS in bulk, a dry powder that comes in >quantity >to make 50 liters, goes into solution rapidly, from Sigma. Do not buy >the >DPBS with Mg or Ca added. Weigh out 10 grams into 1 liter of >distilled >water, pH is 7.4, works like a charm and very economical. You can >make up >to 5 or 10 liters at a time, so you have a slightly greater volume of >buffer sitting around, it is stable. Or keep a 10X solution on hand so >you >can dilute it when needed, volume to volume, much faster than weighing >and >waiting. > >How do you fix your FS for IHC work? Time in fixative? Put your just >cut >FS in front of a small fan (you can try different times, i.e. 15 min >versus >30 min, and use PLUS CHARGE slides, to improve drying. Losing a needed > >section is certainly frustrating. Heat application may destroy >antigenic >epitopes. > > > >At 12:44 PM 7/20/2006, you wrote: > >Hi. Just have a few questions for those of you that do >immunofluorescence. > >Typically I do mostly skin punches and shaves for our dermatopath. > >My question > >is, do any of you order the PBS that is used for the procedure or make > it as > >needed? I was making it up as needed, but there has been an increase >in the > >cases and I wondered if anyone ordered commercially. If so, please let >me > >know a good vendor and I will get some ordered. Nothing worse that > >starting to > >process your specimen just to notice that someone left you an empty >bottle > >of > >PBS. I've only been doing this procedure for a few months and I am >always > >looking for little tips to improve my technique. So far things have >been > >going > >well. I had a problem with tissue washing off before applying the >antibodies > >but seem to have improved that area. I have tried applying heat, >extending > >drying times etc., but inevitably there is still some tissue loss. I >put > >several > >sections on each slide with the control and seem to lose usually 1 >section of > > the patient tissue. I would love to have all sections stay until >the > >coverslipping stage but maybe that's my OCD and not realistic. > > > >Thanks for any info or words of wisdom you can share with me. > > > > > > > >Jodi L. Putnam > >HT (ASCP) > >St. Thomas Hospital > >Nashville, Tennessee > >615-389-9930 > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >Gayle Callis >MT,HT,HTL(ASCP) >Research Histopathology Supervisor >Veterinary Molecular Biology >Montana State University - Bozeman >PO Box 173610 >Bozeman MT 59717-3610 >406 994-6367 >406 994-4303 (FAX) > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From lksell <@t> aol.com Fri Jul 21 11:41:16 2006 From: lksell <@t> aol.com (lksell@aol.com) Date: Fri Jul 21 11:41:26 2006 Subject: [Histonet] Cutting frozens Message-ID: <8C87AFB9F0946E4-5E8-799@MBLK-M09.sysops.aol.com> Are non certified techs allowed to cut frozens? ________________________________________________________________________ Check out AOL.com today. Breaking news, video search, pictures, email and IM. All on demand. Always Free. From Annette_hall <@t> pa-ucl.com Fri Jul 21 11:44:30 2006 From: Annette_hall <@t> pa-ucl.com (Annette Hall) Date: Fri Jul 21 11:44:40 2006 Subject: [Histonet] FW: Linscott's Directory(revised message) Message-ID: <9FC023A4AB52BB4D87DC6456081A822C070B11@mercury.pa-ucl.com> No apostrophe http://www.linscottsdirectory.com/index.php -----Original Message----- From: Sherwood, Margaret [mailto:MSHERWOOD@PARTNERS.ORG] Sent: Thursday, July 20, 2006 3:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Linscott's Directory(revised message) I just tried clicking on the website listed below and I get the Microscopy ListServer! Just google Linscott's Directory if you have trouble accessing it. Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) msherwood@partners.org -----Original Message----- From: Sherwood, Margaret Sent: Thursday, July 20, 2006 4:17 PM To: 'histonet@lists.utsouthwestern.edu' Subject: Linscott's Directory To all: I have noticed a lot of inquiries re: specific antibodies and where to order them. There is a wonderful directory (available on-line only now) that lists antibodies and where to order them. Please see: www.linscott'sdirectory.com Should answer many, if not all, of the questions posted. Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) msherwood@partners.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Jul 21 11:51:36 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Jul 21 11:51:41 2006 Subject: [Histonet] Cutting frozens In-Reply-To: <8C87AFB9F0946E4-5E8-799@MBLK-M09.sysops.aol.com> Message-ID: <20060721165136.88733.qmail@web61216.mail.yahoo.com> Absolutely NOT (at least in Florida). Consider that this is one of the most delicate tasks in the histology lab. The patient is waiting in OR, the pathologist and the surgeon have to take a decision based on the FS section. That tissue has to be assured total integrity; this is nothing to be handled by somebody not certified; it has to be also very well trained. Ren? J. lksell@aol.com wrote: Are non certified techs allowed to cut frozens? ________________________________________________________________________ Check out AOL.com today. Breaking news, video search, pictures, email and IM. All on demand. Always Free. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Music Unlimited - Access over 1 million songs.Try it free. From Karen.Heckford <@t> CHW.edu Fri Jul 21 12:13:32 2006 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Fri Jul 21 12:24:08 2006 Subject: [Histonet] CD10 Message-ID: Happy Friday Everyone, Does anyone know what CD10 works the best on FFPE with Dako's Envision Dual Link? Cheers, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathololgy Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu From Tracey.Lenek <@t> CLS.ab.ca Fri Jul 21 12:31:28 2006 From: Tracey.Lenek <@t> CLS.ab.ca (Tracey.Lenek@CLS.ab.ca) Date: Fri Jul 21 12:31:33 2006 Subject: [Histonet] Wescadyne Message-ID: <4BC300747AF87A48BCDF8E48BC2885CE02C1DA49@mail1.calgary.com> Hi, Has anyone ever heard of the chemical "Wescadyne" and if so, the name of the supplier? Thanks for your help. Tracey Lenek CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. From vbaker60 <@t> yahoo.com Fri Jul 21 12:31:28 2006 From: vbaker60 <@t> yahoo.com (Victoria Baker) Date: Fri Jul 21 12:31:34 2006 Subject: [Histonet] Cutting frozens In-Reply-To: <20060721165136.88733.qmail@web61216.mail.yahoo.com> Message-ID: <20060721173128.75526.qmail@web52515.mail.yahoo.com> In smaller community or non-teaching based labs it isn't uncommon for both histotechs and lab assistants to cut frozen sections. Teaching a lab assistant or even a student to cut frozens takes time and practice. They also have to be approved by the pathologists that they will be cutting the frozens for. All of this is documented before anyone is cleared to cut actual cases (this includes new hire histotechs). In teaching hospitals residents have to cut their own frozens. These residents usually learn from other residents and histologists that are trained to cut frozens. They too have to be approved by the pathologists before they can start cutting actual cases. >From what I've seen having a trained lab assistant or 1st year resident handle a frozen hasn't had adverse affects on any patient or their care. As the saying goes, everyone has to learn some how and the best way to learn is from someone who knows what their doing. Vikki Baker --- Rene J Buesa wrote: > Absolutely NOT (at least in Florida). > Consider that this is one of the most delicate > tasks in the histology lab. The patient is waiting > in OR, the pathologist and the surgeon have to take > a decision based on the FS section. That tissue has > to be assured total integrity; this is nothing to be > handled by somebody not certified; it has to be also > very well trained. > Ren? J. > > lksell@aol.com wrote: > Are non certified techs allowed to cut frozens? > ________________________________________________________________________ > Check out AOL.com today. Breaking news, video > search, pictures, email and IM. All on demand. > Always Free. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Yahoo! Music Unlimited - Access over 1 million > songs.Try it free. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From TJJ <@t> Stowers-Institute.org Fri Jul 21 12:41:07 2006 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Fri Jul 21 12:41:27 2006 Subject: [Histonet] Re: Immunofluorescence question Message-ID: Ditto the recommendation on making up a 10X solution of buffer, and diluting liters or gallons worth as working buffer. Unless I'm mistaken, I believe the immunofluorescent staining she is doing are the direct IF markers on skin biopsies (IgG, IgA, IgM, C3, maybe fibrinogen). In my clinical days, we routinely did those using PBS buffer as a diluent, and used PBS as a wash buffer. We did not use any detergent. As these samples are not fixed (they are sent in Zeus/Michel's transport medium), I would be concerned about the detergent washing away the target before the antibody can label it. If you have extra material and good positive control, try using PBS and TBS with and without tween and see if it makes a difference. As for keeping the section on the glass, we always used positive-charged slides. What micron thickness are your samples? And how long do you let them air-dry prior to doing your immunofluorescent staining? Thicker samples that have not adequately dried onto the slide might be part of the reason your samples are coming off the glass as well. Hope this helps! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From RSRICHMOND <@t> aol.com Fri Jul 21 12:44:29 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Jul 21 12:44:42 2006 Subject: [Histonet] Giemsa stain - Helicobacter Message-ID: <4a7.5630971.31f26c7d@aol.com> Elisa Kelly at Kingston General Hospital, Kingston, Ontario asks about Diff-Quik staining of Helicobacter pylori. Diff-Quik is a trade name. There are two staining solutions. For Helicobacter you need only the blue one (Diff-Quik II). There are a number of generic equivalents for Diff-Quik. In my personal experience they stain Helicobacter pylori quite as well as Diff-Quik II. Don't stain additionally with the red stain (Diff-Quik I) - bacteria are easier to find without it. - The only trick to doing the stain is to get the slide through the alcohols rapidly. Bob Richmond Knoxville TN and Gastonia NC From michael.owen <@t> fda.hhs.gov Fri Jul 21 12:45:12 2006 From: michael.owen <@t> fda.hhs.gov (Owen, Michael P) Date: Fri Jul 21 12:45:22 2006 Subject: [Histonet] Wescodyne Message-ID: Dear Tracey, Wescodyne is the brand name an iodine-based disinfectant produced by Steris Corporation. Steris Corporation: Wescodyne http://www.steris.com/explore/view_product_page.cfm?productid=109 Make sure you are follow the directions on the label and you investigate the effectiveness of Wescodyne on the target organism(s). Your facility's biological safety officers can discuss this issue with you. The Steris Web site has a nice chart that summarizes disinfectants although the chart is focused on Steris products. Steris Corporation / Company Information / Resources http://www.steris.com/resources/lit/literaturesearch.cfm?product2=Wescodyne% 20Germicidal%20Detergent# A great resource on this subject is American Biological Safety Association (ABSA). American Biological Safety Association (ABSA) http://www.absa.org Please e-mail me if you have further questions either on the list or directly to my account. Sincerely, Lab Rat Michael P. Owen, Regulatory Microbiologist U.S. FDA Pacific Regional Lab Northwest 22201 23rd Drive SE Bothell, WA 98021-4421 Phone: 425-483-4865 E-Mail: michael.owen@fda.hhs.gov From vazquezr <@t> ohsu.edu Fri Jul 21 13:00:28 2006 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Jul 21 13:00:56 2006 Subject: [Histonet] Cutting frozens Message-ID: Rene, I beg to differ, I run a Mohs surgery lab and I am uncertified. I train all my people in the arts of cutting tissue, paraffin and frozen, and we have integrity of the most highest standards and we are competent to boot. Robyn OHSU From gcallis <@t> montana.edu Fri Jul 21 13:11:00 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Jul 21 13:11:11 2006 Subject: [Histonet] Cutting frozens In-Reply-To: <8C87AFB9F0946E4-5E8-799@MBLK-M09.sysops.aol.com> References: <8C87AFB9F0946E4-5E8-799@MBLK-M09.sysops.aol.com> Message-ID: <6.0.0.22.1.20060721120715.01b3e988@gemini.msu.montana.edu> You did not specifiy research or clinical laboratory?? In a research laboratory, yes all the time, graduate students learn this too. Clinical laboratories have different requirements, but also what about technicians who are certification elgible, or in working towards the certification, aren't they also allowed to do frozen sections with on the job training with HT eligibility? Medical school residents are trained to do this too. At 10:41 AM 7/21/2006, you wrote: > Are non certified techs allowed to cut frozens? >________________________________________________________________________ >Check out AOL.com today. Breaking news, video search, pictures, email and >IM. All on demand. Always Free. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From mauger <@t> email.chop.edu Fri Jul 21 13:24:55 2006 From: mauger <@t> email.chop.edu (Joanne Mauger) Date: Fri Jul 21 13:25:38 2006 Subject: [Histonet] CD10 Message-ID: Karen, We use a good CD10 (calla) from Vector#VP-C328. Works on FFPE human tissue using citra buffer pH6 for retrieval. Don't know about the Dako link. Jo >>> "Heckford, Karen - SMMC-SF" 07/21/06 1:13 PM >>> Happy Friday Everyone, Does anyone know what CD10 works the best on FFPE with Dako's Envision Dual Link? Cheers, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathololgy Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu From crains <@t> wpmpath.com Fri Jul 21 13:27:31 2006 From: crains <@t> wpmpath.com (crains@wpmpath.com) Date: Fri Jul 21 13:27:39 2006 Subject: [Histonet] Cutting frozens Message-ID: <20060721182733.MGOI3326.dukecmmtao03.coxmail.com@dukecmmtao03> Our Pathologists all insist on doing their own cutting for frozens. > > From: lksell@aol.com > Date: 2006/07/21 Fri AM 11:41:16 CDT > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Cutting frozens > > Are non certified techs allowed to cut frozens? > ________________________________________________________________________ > Check out AOL.com today. Breaking news, video search, pictures, email and IM. All on demand. Always Free. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Important: This email and any attachments may contain confidential information subject to protection under the Federal Standards for Privacy of Individually Identifiable Health Information (45 C.F.R. Parts 160 and 164). If you or your organization is a "Covered Entity" under the above mentioned regulations, you are obligated to treat such information in a manner consistent with the regulations. If it appears that this email was sent to you in error, (1) you are prohibited from utilizing or disseminating this email or any attachments; (2) please immediately delete it from your computer and any servers or other locations where it might be stored and email (crains@wpmpath.com) or call (Chris Rains) at 785-823-7201 advising that you have done so. We appreciate your cooperation. From PMonfils <@t> Lifespan.org Fri Jul 21 13:42:06 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Jul 21 13:42:13 2006 Subject: [Histonet] Linscott's Directory Message-ID: <09C945920A6B654199F7A58A1D7D1FDE01717766@lsexch.lsmaster.lifespan.org> A more valuable resource than Linscott's is www.antibodyresource.com This site includes many excellent listings, including Linscott's, Abcam, Biocompare, and several others. If you can't find an antibody here, they just don't make it! From Rcartun <@t> harthosp.org Fri Jul 21 13:57:53 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Jul 21 13:58:42 2006 Subject: [Histonet] coxiella burnetti In-Reply-To: References: Message-ID: <44C0EB71020000770000101A@hcnwgwds01.hh.chs> Try the Depaprtment of Pathology at Johns Hopkins University in Baltimore, MD. Their telephone number is (410) 955-2405. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Perry, Margaret" 07/20/06 2:20 PM >>> Does anyone know of a lab that does PCR on FFPE for Coxiella burnetti? I would also be interested in any other test for Coxiella on FFPE other than IHC. Margaret Perry South Dakota State University Brookings SD _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Fri Jul 21 14:09:32 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Jul 21 14:09:41 2006 Subject: [Histonet] histotech needed near Charlotte NC Message-ID: <55d.32bf9dc.31f2806c@aol.com> We are in rather urgent need of a full time permanent histotechnologist (one tech leaving to take care of her parents elsewhere) and also of a temporary histotechnologist (one tech going on medical leave) - in Gastonia NC. We would prefer that the temporary work late night to early morning, but that's not essential. Prefer a certified histotechnologist, but will consider uncertified with three years experience. We are not in a position to offer OJT. As many of you know, I'm one of five pathologists serving Gaston Memorial Hospital (see www.caromont.org), a 432 bed community hospital. We normally have five histotechnologists. Our histotechnologists accession specimens into CoPath and MYSIS, but do not gross (we have a pathologist's assistant), do cytopreparation, or assist at autopsies. Two histotechnologists work each Saturday, but no night or emergency call (pathologists do that). We have a Ventana immunostainer. Gastonia is a pleasant small city west of Charlotte, North Carolina. You could commute in either direction, and Gastonia is actually more convenient to the Charlotte airport than most of Charlotte is, right up Interstate 85. In addition to the readily accessible cultural attractions of Charlotte, we have the Daniel Stowe Botanical Garden (one of the nation's outstanding ones), the Schiele Museum of Natural History (which includes a planetarium), and my special thing, the Gastonia Greenway (just look up "greenwaybob" - one word - in Google - for my Web page about it). Telephone 704-831-2173, human resources, and ask for the recruiter, Betsy Eaves. You can contact me by e-mail, but I'm not really involved in the hiring process, and cannot discuss salary or benefits. Robert S. Richmond MD staff pathologist at Gaston Memorial Hospital From JMansfield <@t> cri-inc.com Fri Jul 21 14:20:54 2006 From: JMansfield <@t> cri-inc.com (Mansfield, James) Date: Fri Jul 21 14:20:59 2006 Subject: [Histonet] confocal condition for Cy2 and Cy3 IHC on plants Message-ID: > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf > Of Moran Oliva > Sent: Thursday, July 20, 2006 2:09 PM > To: histonet@lists.utsouthwestern.edu > Cc: Moran Oliva > Subject: [Histonet] confocal condition for Cy2 and Cy3 IHC on plants > > Hello all! > I've just joined this forum. It is very useful. > I've started a preliminary assay of Immunohistochemistry on > Arabidopsis tissue (Steedman's protocol) using my GFP-protein > transgenic plants as a positive control (to confirm the > method is working for me). > The anti-GFP antibodies I used were either from mouse or from > rabbit. So when I used the Cy2 goat anti-mouse I needed to > use the same emission spectra as for the GFP protein itself- > Does anyone knows whether the GFP survive all this process? > does anyone has any good advise of detecting the IHC signal > and not theprotein GFP signal? the second channel I used was > GFP autoflouresence... > Does anyone know what are the confocal setting details for > Cy3 in plant tissue? I've tried to detect the GFP in IHC > using rabbit GFP protein, so my second antibody is Cy3 > conjugated ( I know the excitation and emission spectra, but > the autoflourescence is very close to its wave lengths-do I > need to use a second channel for autoflourescence in this case too? Hi Moran, A good place to find wavelengths for fluorophores is the Curv-omatic site from Omega Optical: https://www.omegafilters.com/front/curvomatic/spectra.php It will show you absorption and emission spectra for almost any fluorophores you might want to use. However, having said that, separating fluorescence from your fluorophore from autofluorescence can be very difficult for most systems. Autofluorescence emits at almost all wavelengths, and will conflict with just about any filter set you want to try. A multispectral imaging approach can help a lot in eliminating autofluorescence from your samples, with signal-to-noise increases of 100-fold. Here is a link to a paper we are publishing in Cytometry later this year (two issues from now, I believe) that discusses using multispectral imaging to separate fluorophores from autofluorescence. The examples are done using our own spectral imaging system (Nuance), but the approach applies in general. http://www.cri-inc.com/files/MSI_App_02.pdf Cheers, -Jim From gcallis <@t> montana.edu Fri Jul 21 14:45:43 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Jul 21 14:45:56 2006 Subject: [Histonet] Fluorophore adsorption emission and spectral separation In-Reply-To: References: Message-ID: <6.0.0.22.1.20060721133827.01b346e8@gemini.msu.montana.edu> Moran, Molecular Probes, go to resources on home page for MP, and click on their spectraimager. You can play with the fluorophores you need to see (do it as antibodies) and see the graphs for separation - for both CLSM and fluorescent microscope work. With our confocal, adjustment can be made to eliminate autofluorescence. I also have a review article on autofluorescence, written with GFP in mind, but useful for immunofluorescent work also. If you want the review, I will send via private email. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From JGREWE <@t> OhioHealth.com Fri Jul 21 14:54:40 2006 From: JGREWE <@t> OhioHealth.com (JGREWE@OhioHealth.com) Date: Fri Jul 21 14:54:50 2006 Subject: [Histonet] Jacquelyn Grewe/Staff/OhioHealth is out of the office . Message-ID: I will be out of the office starting 07/21/2006 and will not return until 07/31/2006. I will respond to your message when I return. Thanks, Jackie From RSRICHMOND <@t> aol.com Fri Jul 21 15:24:26 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Jul 21 15:24:36 2006 Subject: [Histonet] Histotech needed near Charlotte NC Message-ID: I apologize for having given an incorrect phone number for our H.R. service at Gaston Memorial Hospital, where, I noted earlier, we are looking for a histotechnologist. It's 704-834-2141 or 704-834-2173. Bob Richmond From Linresearch <@t> aol.com Sat Jul 22 05:02:46 2006 From: Linresearch <@t> aol.com (Linresearch@aol.com) Date: Sat Jul 22 05:02:56 2006 Subject: [Histonet] Blocks/hr Message-ID: <3b9.66993bd.31f351c6@aol.com> Hi, Does anyone know if ASCP has established a guideline for the number of blocks/hr that Histotechnicians are expected to cut? Would you share the number that your lab has established for your technicians? Thanks From rjbuesa <@t> yahoo.com Sat Jul 22 07:38:39 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sat Jul 22 07:38:46 2006 Subject: [Histonet] Blocks/hr In-Reply-To: <3b9.66993bd.31f351c6@aol.com> Message-ID: <20060722123839.38403.qmail@web61211.mail.yahoo.com> Under separate cover I am sending you a "pdf" file of a paper I just published (3 July) in Advance on the same subject. CAP in a report from 2002 recommended an equivalent of 17 blocks/h when cut for the first time and an average of 30 blocks/hour for each additional section. This figure is 1.8 times lower than the average of 31 blocks/hour from the survey (as you will see in my article). Hope this will help you. Ren? J. Linresearch@aol.com wrote: Hi, Does anyone know if ASCP has established a guideline for the number of blocks/hr that Histotechnicians are expected to cut? Would you share the number that your lab has established for your technicians? Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Groups are talking. We´re listening. Check out the handy changes to Yahoo! Groups. From marjoh3 <@t> telus.net Sat Jul 22 09:46:39 2006 From: marjoh3 <@t> telus.net (Marilyn Johnson) Date: Sat Jul 22 09:46:40 2006 Subject: [Histonet] Commercial Decalcifying Solns. Message-ID: <001601c6ad9d$a4126ee0$6401a8c0@VALUED20606295> Hi Histonetters, Could anyone provide me with their preferences for commercially prepared decalcifying solns? These solns will be required for routine bone sections for H&E staining and not biopsies or rush specimens. Please provide company names and order numbers. Thank you in advance for any replies. Marilyn Johnson Alberta Agriculture Edmonton, AB. Canada. From Sunita.Ho <@t> ucsf.edu Sun Jul 23 15:54:35 2006 From: Sunita.Ho <@t> ucsf.edu (Ho, Sunita) Date: Sun Jul 23 15:54:51 2006 Subject: [Histonet] Gomori's Trichrome on Cementum of a Human Tooth Message-ID: <0D6FC3C553E12A43B7C6B1F4A9DA429301F60D8B@EXVS05.net.ucsf.edu> Hi All, I recently stained a decalcified human cementum tissue with Gomori's Trichrome (using a standard procedure provided in any histology text book). I have regions of red and blue - Cementum was predominantly stained red and dentin blue (consistently observed between many specimens). I am assuming that this was primarily due to packing density of collagen, more in cementum than in dentin. The cementum around the lacunae was stained blue. Is my assumption correct or is there a better technical answer for what I observed using a light microscope? I look forward to your thoughts. Thanks in advance. Sunita From a.schmidt <@t> fuse.net Sun Jul 23 18:46:32 2006 From: a.schmidt <@t> fuse.net (a.schmidt@fuse.net) Date: Sun Jul 23 18:46:38 2006 Subject: [Histonet] Rapid Tissue Processor Message-ID: <22333582.1153698392694.JavaMail.root@webmail6> We have been using the Sakura Rapid Tissue processor for about 3 months now. We have been having trouble with our digested PAS stain on liver needle biopsies using the Ventana Nexes stainer. Has any other facility experinced any problems with their special stains or IHC stains and if you have this processor I would appreciate hearing any of your feelings about it. Tahkns for the input. Angie Schmidt Tri-Health Labs--Cincinnati From kaleid11 <@t> yahoo.com Mon Jul 24 09:47:45 2006 From: kaleid11 <@t> yahoo.com (Adam Perry) Date: Mon Jul 24 09:47:49 2006 Subject: [Histonet] Fluorophore adsorption emission and spectral separation In-Reply-To: <6.0.0.22.1.20060721133827.01b346e8@gemini.msu.montana.edu> Message-ID: <20060724144745.41330.qmail@web30401.mail.mud.yahoo.com> I had an immunofluorescence question that I'd love to get some input on from anyone out there. I'm trying to colocalize two antigens in rodent brain sections. One of the antigens is yielding fairly weak staining- it also happens to be the one that I'm visualizing with Alexa Fluor 488, so I'm getting fairly high background autofluorescence- and I think that the staining could be improved by reducing the autofluorescence. My DAPI staining (ex/em: 350/470) is great...it only stains nuclei with minimal autofluorescence. They offer Alexa Fluor 405 streptavidin which has a similar excit/emission range, so I thought labeling with the Alexa Fluor 405 would produce a similarly "clean" background as my DAPI labeling. Also, I already have the filter sets to work with Alexa Fluor 405 (as opposed to going to the other end of the spectrum). Has anyone used Alexa Fluor 405? What is your impression of tissue autofluorescence in that range, photostability/bleaching of the compounds, comparison of signal using other fluorophores, etc. Any input would be greatly appreciated. Thanks, Adam Perry Department of Neuroscience University of Illinois at Chicago Chicago, IL 60612 Gayle Callis wrote: Moran, Molecular Probes, go to resources on home page for MP, and click on their spectraimager. You can play with the fluorophores you need to see (do it as antibodies) and see the graphs for separation - for both CLSM and fluorescent microscope work. With our confocal, adjustment can be made to eliminate autofluorescence. I also have a review article on autofluorescence, written with GFP in mind, but useful for immunofluorescent work also. If you want the review, I will send via private email. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail Beta. From pruegg <@t> ihctech.net Mon Jul 24 10:19:49 2006 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Mon Jul 24 10:19:50 2006 Subject: [Histonet] Commercial Decalcifying Solns. In-Reply-To: <001601c6ad9d$a4126ee0$6401a8c0@VALUED20606295> Message-ID: <200607241519.k6OFJhlG059684@pro12.abac.com> I prefer Immunocal by Decal Chemicals, it seems to be just as fast as the more harsh HCL based decals like RDO but much much better for IHC, it is formic acid based. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. #216 Aurora, CO 80010 720-859-4060 fax 720-859-4110 pruegg@ihctech.net www.ihctech.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marilyn Johnson Sent: Saturday, July 22, 2006 8:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Commercial Decalcifying Solns. Hi Histonetters, Could anyone provide me with their preferences for commercially prepared decalcifying solns? These solns will be required for routine bone sections for H&E staining and not biopsies or rush specimens. Please provide company names and order numbers. Thank you in advance for any replies. Marilyn Johnson Alberta Agriculture Edmonton, AB. Canada. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jessgrocki <@t> yahoo.com Mon Jul 24 11:17:45 2006 From: jessgrocki <@t> yahoo.com (Jessica Piche) Date: Mon Jul 24 11:17:50 2006 Subject: [Histonet] IHC validation of blocks and anitbodies Message-ID: <20060724161745.56186.qmail@web82011.mail.mud.yahoo.com> Hi All, I have a few questions regarding IHC. When validating controls and new antibodies do you always run a negative control slide as well? Can anyone share their protocols with me on validating controls, and new antibodies? Any help would be appreciated. Thank you. Jessica Piche-Grocki, HT (ASCP) From MadaryJ <@t> MedImmune.com Mon Jul 24 11:22:19 2006 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Mon Jul 24 11:22:28 2006 Subject: [Histonet] CAE and Mature Mast cells look at procedure Message-ID: <7CAB706201F11843BD26AD516326F0C8010D3890@MD1MS007.medimmune.com> Okay my histoheroes and heroines, One of the investigators here asked for a CAE designed for plastics(but supposedly works on FFPE). I did this protocol, but it isn't working. I am having a rough time because I am not used to making mistakes(kidding). She needs mature mast cells only, I gave her 7 different Mast cell procedures that all worked very well(even the dominicis), and one Alcian Blue Safranin that stains Heparin containing Mast cells red Amine containing Mast cells blue. So 2 questions are: 1-Do mature cells stain differently? 2-Does anyone have a decent CAE they can share? Here is the one I used and the control is fresh rat skin and all the other procedures worked on that block. Chloroacetate Esterase (CAE) Staining Preparation of Solutions 1. New Fuchsin Solution New Fuchsin 1g 2N hydrochloric acid (HCl) 25ml 2. 4% Sodium Nitrite Solution Sodium Nitrite 1g ddH2O 25ml 3. Phosphate Buffer (0.1M, pH 7.6) Stock Solutions A. 0.2M solution of monobasic soduim phosphate (27.8g NaH2PO4 in 1L H2O) B. 0.2M solution of dibasic sodium phosphate (28.4g Na2HPO4 in 1L H2O) --> To make pH 7.6 Solution: mix 6.5mL of A, 43.5mL of B, and 50mL of ddH2O. 4. Naphthol AS-D Chloroacetate Solution (Store at -20?C) Naphthol AS-D Chloroacetate 10mg 100mg N-N Dimethyl Formamide 5ml 50ml Staining Solution (make fresh before each use) In a single vial: 1) mix New Fuchsin and 4% Soduim Nitrite 2) add phosphate buffer and mix 3) add Naphth AS-D Chloroacetate Soultion and mix well Use 1 ml freshly mixed solution for approx 4 tissue slides New Fuchsin 2.5?l 5?l 12.5?l 25?l 4% Sodium Nitrite 2.5?l 5?l 12.5?l 25?l Phosphate Buffer 1ml 2ml 5ml 10ml Naphthol AS-D Chloroacetate 50?l 100?l 250?l 500?l Staining Procedure 1. Stain with fresh staining solution for 20 min. 2. Wash with running tap water (For plastic sections use wet cotton swab wipe off dusty residue). 3. Counterstain with Gill's II hematoxylin for 20 to 45 seconds (or methyl green for 30 seconds). 4. Wash with warm tap water to blue (longer will result darker blue, or dip into 50% of lithium carbonate for 5 - 20 seconds for deeper blue, then rinse with water). Dry and mount with mounting medium. (For paraffin sections, quick dehydration before mounting). From schloesr <@t> mail.nih.gov Mon Jul 24 11:41:50 2006 From: schloesr <@t> mail.nih.gov (Robert J. Schloesser) Date: Mon Jul 24 11:40:09 2006 Subject: [Histonet] Postfixation of frozen brain tissue Message-ID: <000001c6af40$0ff4ff80$76e4bb89@yourd54swmkxh6> Hi All, We have to do IHC on fresh frozen brain samples perfused only with saline. I am only used to cutting and IHC on PFA fixed brains. Therefore I have many questions on how to treat fresh frozen samples. Postfixation on slides: PFA or Acetone/Ethanol? Is there a reliable way to postfix whole blocks? Is there a way to postfix 30-200?m thick slides without mounting them on slide (to do free floating staingin)? Any kind of input, protocols or tips&tricks and do`s and don`t are highly appreciated. Thank you very much Robert From settembr <@t> umdnj.edu Mon Jul 24 11:44:48 2006 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Mon Jul 24 11:45:57 2006 Subject: [Histonet] Can Alk phs be run on peroxidase slides? Message-ID: Hi, One of my pathologists is asking if I can take a slide that has already been stained with a peroxidase/avidin/biotin/DAB detection system and re-stain with an Alkaline Phosphotase detection system. Can it work? Must it be de-stained? Any info will help. Thanks, Dana Settembre Immunohistochemistry Lab University Hospital - UMDNJ Newark, NJ From melissa.mazan <@t> tufts.edu Mon Jul 24 12:23:13 2006 From: melissa.mazan <@t> tufts.edu (Melissa Mazan) Date: Mon Jul 24 12:23:20 2006 Subject: [Histonet] Re: Histonet Digest, Vol 32, Issue 24 In-Reply-To: <200607241706.k6OH6GdI022216@mail-proofpoint-2a.usg.tufts.edu> References: <200607241706.k6OH6GdI022216@mail-proofpoint-2a.usg.tufts.edu> Message-ID: <44C50201.5090600@tufts.edu> Hi all, Can those of you who do non-automated dewaxing share their protocols with me? At first I was doing 3X 3 minutes xylene, then 1X 2 minutes 100% EtOH, then one minute of 85%, and one minute of 75%. Having problems with antigen labeling (immunofluorescence) so am worried that it might be my dewaxing protocol. Also, how often do you completely change out the xylene? Thanks in advance - Melissa histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Gomori's Trichrome on Cementum of a Human Tooth (Ho, Sunita) > 2. Rapid Tissue Processor (a.schmidt@fuse.net) > 3. Re: Fluorophore adsorption emission and spectral separation > (Adam Perry) > 4. RE: Commercial Decalcifying Solns. (patsy ruegg) > 5. IHC validation of blocks and anitbodies (Jessica Piche) > 6. CAE and Mature Mast cells look at procedure (Madary, Joseph) > 7. Postfixation of frozen brain tissue (Robert J. Schloesser) > 8. Can Alk phs be run on peroxidase slides? (Dana Settembre) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sun, 23 Jul 2006 13:54:35 -0700 > From: "Ho, Sunita" > Subject: [Histonet] Gomori's Trichrome on Cementum of a Human Tooth > To: histonet@lists.utsouthwestern.edu > Message-ID: > <0D6FC3C553E12A43B7C6B1F4A9DA429301F60D8B@EXVS05.net.ucsf.edu> > Content-Type: text/plain; charset=us-ascii > > Hi All, > > I recently stained a decalcified human cementum tissue with Gomori's > Trichrome (using a standard procedure provided in any histology text > book). I have regions of red and blue - Cementum was predominantly > stained red and dentin blue (consistently observed between many > specimens). I am assuming that this was primarily due to packing > density of collagen, more in cementum than in dentin. The cementum > around the lacunae was stained blue. Is my assumption correct or is > there a better technical answer for what I observed using a light > microscope? I look forward to your thoughts. > > Thanks in advance. > > Sunita > > > > > ------------------------------ > > Message: 2 > Date: Sun, 23 Jul 2006 19:46:32 -0400 > From: > Subject: [Histonet] Rapid Tissue Processor > To: Histonet@lists.utsouthwestern.edu > Message-ID: <22333582.1153698392694.JavaMail.root@webmail6> > Content-Type: text/plain; charset=utf-8 > > We have been using the Sakura Rapid Tissue processor for about 3 months now. We have been having trouble with our digested PAS stain on liver needle biopsies using the Ventana Nexes stainer. Has any other facility experinced any problems with their special stains or IHC stains and if you have this processor I would appreciate hearing any of your feelings about it. Tahkns for the input. > Angie Schmidt > Tri-Health Labs--Cincinnati > > > > ------------------------------ > > Message: 3 > Date: Mon, 24 Jul 2006 07:47:45 -0700 (PDT) > From: Adam Perry > Subject: Re: [Histonet] Fluorophore adsorption emission and spectral > separation > To: Histonet@lists.utsouthwestern.edu > Message-ID: <20060724144745.41330.qmail@web30401.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > I had an immunofluorescence question that I'd love to get some input on from anyone out there. I'm trying to colocalize two antigens in rodent brain sections. One of the antigens is yielding fairly weak staining- it also happens to be the one that I'm visualizing with Alexa Fluor 488, so I'm getting fairly high background autofluorescence- and I think that the staining could be improved by reducing the autofluorescence. My DAPI staining (ex/em: 350/470) is great...it only stains nuclei with minimal autofluorescence. They offer Alexa Fluor 405 streptavidin which has a similar excit/emission range, so I thought labeling with the Alexa Fluor 405 would produce a similarly "clean" background as my DAPI labeling. Also, I already have the filter sets to work with Alexa Fluor 405 (as opposed to going to the other end of the spectrum). > > Has anyone used Alexa Fluor 405? What is your impression of tissue autofluorescence in that range, photostability/bleaching of the compounds, comparison of signal using other fluorophores, etc. > > Any input would be greatly appreciated. > Thanks, > Adam Perry > > Department of Neuroscience > University of Illinois at Chicago > Chicago, IL 60612 > > > Gayle Callis wrote: > Moran, > > Molecular Probes, go to resources on home page for MP, and click on their > spectraimager. You can play with the fluorophores you need to see (do it > as antibodies) and see the graphs for separation - for both CLSM and > fluorescent microscope work. > > With our confocal, adjustment can be made to eliminate > autofluorescence. I also have a review article on autofluorescence, > written with GFP in mind, but useful for immunofluorescent work also. > > If you want the review, I will send via private email. > > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 > 406 994-4303 (FAX) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Do you Yahoo!? > Get on board. You're invited to try the new Yahoo! Mail Beta. > > ------------------------------ > > Message: 4 > Date: Mon, 24 Jul 2006 09:19:49 -0600 > From: "patsy ruegg" > Subject: RE: [Histonet] Commercial Decalcifying Solns. > To: "'Marilyn Johnson'" , > > Message-ID: <200607241519.k6OFJhlG059684@pro12.abac.com> > Content-Type: text/plain; charset="US-ASCII" > > I prefer Immunocal by Decal Chemicals, it seems to be just as fast as the > more harsh HCL based decals like RDO but much much better for IHC, it is > formic acid based. > Patsy > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. #216 > Aurora, CO 80010 > 720-859-4060 > fax 720-859-4110 > pruegg@ihctech.net > www.ihctech.net > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Marilyn > Johnson > Sent: Saturday, July 22, 2006 8:47 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Commercial Decalcifying Solns. > > Hi Histonetters, > Could anyone provide me with their preferences for commercially prepared > decalcifying solns? > These solns will be required for routine bone sections for H&E staining and > not biopsies or rush specimens. > Please provide company names and order numbers. > Thank you in advance for any replies. > > Marilyn Johnson > Alberta Agriculture > Edmonton, AB. Canada. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 5 > Date: Mon, 24 Jul 2006 09:17:45 -0700 (PDT) > From: Jessica Piche > Subject: [Histonet] IHC validation of blocks and anitbodies > To: histonet > Message-ID: <20060724161745.56186.qmail@web82011.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi All, > > I have a few questions regarding IHC. When validating controls and new antibodies do you always run a negative control slide as well? Can anyone share their protocols with me on validating controls, and new antibodies? Any help would be appreciated. Thank you. > > Jessica Piche-Grocki, HT (ASCP) > > > ------------------------------ > > Message: 6 > Date: Mon, 24 Jul 2006 12:22:19 -0400 > From: "Madary, Joseph" > Subject: [Histonet] CAE and Mature Mast cells look at procedure > To: > Message-ID: > <7CAB706201F11843BD26AD516326F0C8010D3890@MD1MS007.medimmune.com> > Content-Type: text/plain; charset="iso-8859-1" > > Okay my histoheroes and heroines, > One of the investigators here asked for a CAE designed for plastics(but supposedly works on FFPE). I did this protocol, but it isn't working. I am having a rough time because I am not used to making mistakes(kidding). She needs mature mast cells only, I gave her 7 different Mast cell procedures that all worked very well(even the dominicis), and one Alcian Blue Safranin that stains Heparin containing Mast cells red Amine containing Mast cells blue. So 2 questions are: > > 1-Do mature cells stain differently? > 2-Does anyone have a decent CAE they can share? Here is the one I used and the control is fresh rat skin and all the other procedures worked on that block. > > Chloroacetate Esterase (CAE) Staining > > Preparation of Solutions > > 1. New Fuchsin Solution > New Fuchsin 1g > 2N hydrochloric acid (HCl) 25ml > > 2. 4% Sodium Nitrite Solution > Sodium Nitrite 1g > ddH2O 25ml > > 3. Phosphate Buffer (0.1M, pH 7.6) > Stock Solutions > A. 0.2M solution of monobasic soduim phosphate (27.8g NaH2PO4 in 1L H2O) > B. 0.2M solution of dibasic sodium phosphate (28.4g Na2HPO4 in 1L H2O) > --> To make pH 7.6 Solution: mix 6.5mL of A, 43.5mL of B, and 50mL of ddH2O. > > 4. Naphthol AS-D Chloroacetate Solution (Store at -20?C) > Naphthol AS-D Chloroacetate 10mg 100mg > N-N Dimethyl Formamide 5ml 50ml > > > Staining Solution (make fresh before each use) > > In a single vial: 1) mix New Fuchsin and 4% Soduim Nitrite > 2) add phosphate buffer and mix > 3) add Naphth AS-D Chloroacetate Soultion and mix well > > Use 1 ml freshly mixed solution for approx 4 tissue slides > New Fuchsin 2.5?l 5?l 12.5?l 25?l > 4% Sodium Nitrite 2.5?l 5?l 12.5?l 25?l > Phosphate Buffer 1ml 2ml 5ml 10ml > Naphthol AS-D Chloroacetate 50?l 100?l 250?l 500?l > > Staining Procedure > 1. Stain with fresh staining solution for 20 min. > 2. Wash with running tap water (For plastic sections use wet cotton swab wipe off dusty residue). > 3. Counterstain with Gill's II hematoxylin for 20 to 45 seconds (or methyl green for 30 seconds). > 4. Wash with warm tap water to blue (longer will result darker blue, or dip into 50% of lithium carbonate for 5 - 20 seconds for deeper blue, then rinse with water). > Dry and mount with mounting medium. (For paraffin sections, quick dehydration before mounting). > > > > ------------------------------ > > Message: 7 > Date: Mon, 24 Jul 2006 12:41:50 -0400 > From: "Robert J. Schloesser" > Subject: [Histonet] Postfixation of frozen brain tissue > To: > Message-ID: <000001c6af40$0ff4ff80$76e4bb89@yourd54swmkxh6> > Content-Type: text/plain; charset="iso-8859-1" > > Hi All, > > We have to do IHC on fresh frozen brain samples perfused only with saline. I > am only used to cutting and IHC on PFA fixed brains. > Therefore I have many questions on how to treat fresh frozen samples. > > Postfixation on slides: PFA or Acetone/Ethanol? > Is there a reliable way to postfix whole blocks? > Is there a way to postfix 30-200?m thick slides without mounting them on > slide (to do free floating staingin)? > > Any kind of input, protocols or tips&tricks and do`s and don`t are highly > appreciated. > > Thank you very much > > Robert > > > > > ------------------------------ > > Message: 8 > Date: Mon, 24 Jul 2006 12:44:48 -0400 > From: "Dana Settembre" > Subject: [Histonet] Can Alk phs be run on peroxidase slides? > To: > Message-ID: > Content-Type: text/plain; charset=US-ASCII > > Hi, > One of my pathologists is asking if I can take a slide that has already > been stained with a > peroxidase/avidin/biotin/DAB detection system and re-stain with an > Alkaline Phosphotase detection system. > Can it work? > Must it be de-stained? > > Any info will help. Thanks, > > Dana Settembre > Immunohistochemistry Lab > University Hospital - UMDNJ > Newark, NJ > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 32, Issue 24 > **************************************** -- Melissa R. Mazan, DVM, Diplomate ACVIM Associate Professor of Large Animal Medicine Director of Sports Medicine Cummings School of Veterinary Medicine Tufts University 200 Westborough Road North Grafton, MA 01536 Tel: 508-839-5395 Fax: 508-839-7922 Email: melissa.mazan@tufts.edu From failm <@t> musc.edu Mon Jul 24 12:33:19 2006 From: failm <@t> musc.edu (Mildred Fail) Date: Mon Jul 24 12:34:44 2006 Subject: [Histonet] IHC validation of blocks and anitbodies Message-ID: We submit a request form for control material with a positive case, the pathologist approves the case , a copy of the signed request is kept in a notebook and the control logged in the control log. Everytime that block is cut the 1st and last slides are tested. When we validate antibodies we do run a negative control. We run 13 slides 3 dilutions, 4 methods 1 negative. Rena Fail >>> Jessica Piche 07/24/06 12:17PM >>> Hi All, I have a few questions regarding IHC. When validating controls and new antibodies do you always run a negative control slide as well? Can anyone share their protocols with me on validating controls, and new antibodies? Any help would be appreciated. Thank you. Jessica Piche-Grocki, HT (ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJJ <@t> Stowers-Institute.org Mon Jul 24 12:40:22 2006 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Mon Jul 24 12:40:37 2006 Subject: [Histonet] Re: Can Alk phs be run on peroxidase slides? Message-ID: Dana, the answer is yes, it can work (anything is possible, right?). It does not have to be destained. Are you looking for colocalization or does the pathologist expect the other antibody complex to be localized elsewhere? Co-localization may be difficult to visualize using DAB and a red or dark blue AP chromogen. I recommend that you include two positive controls for the AP-labeled antibody. On one, do the peroxidase/avidin/biotin/DAB sequence just as your slide has had (regardless of whether there would be any positive labeling with the first antibody), and then run both using the second antibody staining sequence. So, one will have the double stain sequence, and the other just the single AP stain sequence. You should be able to tell then if there has been any diminution of signal due to the previous labeling technique. Chris van der Loos has reported that DAB has a sheltering effect on antigens and may actually help keep the 2nd primary from unwanted binding. >From the archives: "1. Sequential double staining only works because of unique effective sheltering of DAB chromogen. As far as I know there are no other enzymatic chromogens with this sheltering capacity. Always apply DAB after the 1st staining sequence!!!!!" Finally, make sure your second primary antibody is raised in a different species than your first primary antibody. There are ways around it, and we can talk further if that's the case. Good luck! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 Date: Mon, 24 Jul 2006 12:44:48 -0400 From: "Dana Settembre" Subject: [Histonet] Can Alk phs be run on peroxidase slides? To: Message-ID: Content-Type: text/plain; charset=US-ASCII Hi, One of my pathologists is asking if I can take a slide that has already been stained with a peroxidase/avidin/biotin/DAB detection system and re-stain with an Alkaline Phosphotase detection system. Can it work? Must it be de-stained? Any info will help. Thanks, Dana Settembre Immunohistochemistry Lab University Hospital - UMDNJ Newark, NJ Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From TJJ <@t> Stowers-Institute.org Mon Jul 24 12:49:36 2006 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Mon Jul 24 12:49:46 2006 Subject: [Histonet] RE: CAE and Mature Mast cells look at procedure Message-ID: Hi Joseph, I hope John Kiernan, Bryan Llewellyn, and others much more knowledgeable about histochemistry than I, chime in here. A quick search of the archives brought up this: http://www.histosearch.com/histonet/Feb03A/MastcellstainingWasuntitl.htm l Don't know if this helps, but thought it worth a post. Best wishes, Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From Melissa.Gonzalez <@t> cellgenesys.com Mon Jul 24 14:03:36 2006 From: Melissa.Gonzalez <@t> cellgenesys.com (Melissa Gonzalez) Date: Mon Jul 24 14:03:46 2006 Subject: [Histonet] Re: Fluorophore adsorption emission and spectral separation Message-ID: Adam, I've never had much luck visualizing the blue-range emitting dyes. In my experience, they tend to be very weak and photobleach very easily, which are sentiments expressed by others I've conferred with as well. AF488 is usually pretty distinct, so if you are having trouble with this, I would think AF405 or AF35 would be much worse. DAPI is a very strong nucleic dye, and unless your filters are shot, should always look really good. If you have the filters: AF555 (TRITC) or AF594 (Texas red equivalent) are also very bright. Also, I am not sure how thick of sections you are using, but brain tissue structures always pop up a little nicer when you use thicker sections with fluorescence. Sometimes what may appear wimpy on a thin section will stand out on a thicker one. Good luck, Melissa -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Monday, July 24, 2006 10:41 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 32, Issue 24 I had an immunofluorescence question that I'd love to get some input on from anyone out there. I'm trying to colocalize two antigens in rodent brain sections. One of the antigens is yielding fairly weak staining- it also happens to be the one that I'm visualizing with Alexa Fluor 488, so I'm getting fairly high background autofluorescence- and I think that the staining could be improved by reducing the autofluorescence. My DAPI staining (ex/em: 350/470) is great...it only stains nuclei with minimal autofluorescence. They offer Alexa Fluor 405 streptavidin which has a similar excit/emission range, so I thought labeling with the Alexa Fluor 405 would produce a similarly "clean" background as my DAPI labeling. Also, I already have the filter sets to work with Alexa Fluor 405 (as opposed to going to the other end of the spectrum). Has anyone used Alexa Fluor 405? What is your impression of tissue autofluorescence in that range, photostability/bleaching of the compounds, comparison of signal using other fluorophores, etc. Any input would be greatly appreciated. Thanks, Adam Perry Department of Neuroscience University of Illinois at Chicago Chicago, IL 60612 From hhawkins <@t> utmb.edu Mon Jul 24 14:43:56 2006 From: hhawkins <@t> utmb.edu (Hawkins, Hal K.) Date: Mon Jul 24 14:44:01 2006 Subject: [Histonet] paraformaldehyde Message-ID: <8D6F233E2A5D574B929F3944F3316FD009C2BFC5@EXCH2K3.utmb.edu> We need to prepare 4% phosphate-buffered paraformaldehyde for a cooperative study in fairly large volumes, about 2 L at a time. My only experience in preparing this fixative involved heating to over 60 C, adding sodium hydroxide chips until all was dissolved, then adding the buffer and adjusting the pH. There seems to be a simpler method in which pre-mixed Dulbecco PBS is warmed and the paraformaldehyde is dissolved in the buffer. Has anyone used this method and does it work without further adjustment of the final pH? Thanks in advance, Hal Hawkins UTMB, Galveston, Texas From ploykasek <@t> phenopath.com Mon Jul 24 15:14:47 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Mon Jul 24 15:14:56 2006 Subject: [Histonet] NY licensure Message-ID: HI all. I was wondering if anyone has seen the new NY licensure requirements for lab techs? The way I am reading it, it looks like histotechs will be classified as Clinical Laboratory Technologists/Technicians, but I could be wrong. There is a grandfather clause in the law. The law is Article 165, or the Clinical Laboratory Technology Practice Act. I would appreciate input and more information on this new law. Thank you. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From fmonson <@t> wcupa.edu Mon Jul 24 15:17:17 2006 From: fmonson <@t> wcupa.edu (Monson, Frederick ) Date: Mon Jul 24 15:17:36 2006 Subject: [Histonet] paraformaldehyde In-Reply-To: <8D6F233E2A5D574B929F3944F3316FD009C2BFC5@EXCH2K3.utmb.edu> Message-ID: My preference is to prepare 20% formaldehyde in 200-400ml lots (but a liter can also be made!) from paraformaldehyde and keep it in the refrigerator. In my experience, 20% is about as high as I can go, if I want to keep it for a time. What I have copied between the lines is the protocol I use for my own purposes. It can be altered, but none of the safety concerns are changed. ------------------------------------------------------------------------ ---- ALL WORK WITH THESE CHEMICALS SHOULD BE PERFORMED IN A FUNCTIONING CHEMICAL HOOD! ------------------------------------------------------------------------ --- Careful Preparation of Formaldehyde from Paraformaldehyde (PFA), and the Safe Use of pHCHO Thereafter !!!!!DO IT ALL IN A CHEMICAL FUME HOOD!!!!! 1. Weigh 80g paraformaldehyde - (NOTE: this powder must be handled with great care to prevent 'powder splashing' during weighing.) 2. Suspend in 400ml HOH with constant stirring and raise temperature to 50-60oC 3. Add 5 OR 10N NaOH dropwise at 2min intervals until solution begins to clear. Then dropwise at 5min intervals until clearing is maximized. DO NOT BOIL!!!!! 4. Filter on a Beutner funnel with light suction using Whatman #2 paper and store at 4oC in a screw cap bottle. This solution keeps for 1 or more months at 4oC. 5. Portions of aliquots can then be diluted to working solutions as required with buffered saline or other solutions. (NOTE: Working solutions are used and stored at 4oC in appropriate containers.) Dilution of the stock solution is performed quickly and dispensing of the working solution may be accomplished by pouring or pipetting the working solution into vials. Safety concerns: Formaldehyde is a gas which saturates water at about 40%(w/v) at ambient temperature and pressure. Evolution of the gas occurs most readily when concentrated solutions are diluted, and when temperatures are high. IT IS DANGEROUS TO DISPOSE OF 10% OR HIGHER SOLUTIONS OF FORMALDEHYDE IN A SINK! Paraformaldehyde is a polymer of HCHO that depolymerizes under conditions of mild heat (optimum is 60oC with constant stirring)) and alkalinity. Solutions >20%(w/v) tend to repolymerize on standing. Disposal is only permitted in accordance with local, state and federal guidelines. Find out what they are for YOU! Formaldehyde and Paraformaldehyde are NOT synonyms. When in any doubt, ASK questions. Obey LOCAL Regulations!!!! ------------------------------------------------------------------------ -- ALL WORK WITH THESE CHEMICALS SHOULD BE PERFORMED IN A FUNCTIONING CHEMICAL HOOD! ------------------------------------------------------------------------ --- Hope this helps, Frederick C. Monson, PhD Technical Director Light, Electron, X-Ray and Scanning Probe Imaging and Analysis Center (LEXSPIAC) Large Scientific Instrument Core Geology, West Chester University S. Church St. and W. Rosedale Ave. West Chester, PA, 19320 610-738-0437 fmonson@wcupa.edu URL: http://darwin.wcupa.edu/cgi-bin/casireserve.cgi ============================================ Knowledge is the key to happiness. Ignorance might be the key to contentment. ============================================ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hawkins, Hal K. Sent: Monday, July 24, 2006 3:44 PM To: histonet Subject: [Histonet] paraformaldehyde We need to prepare 4% phosphate-buffered paraformaldehyde for a cooperative study in fairly large volumes, about 2 L at a time. My only experience in preparing this fixative involved heating to over 60 C, adding sodium hydroxide chips until all was dissolved, then adding the buffer and adjusting the pH. There seems to be a simpler method in which pre-mixed Dulbecco PBS is warmed and the paraformaldehyde is dissolved in the buffer. Has anyone used this method and does it work without further adjustment of the final pH? Thanks in advance, Hal Hawkins UTMB, Galveston, Texas _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jdr43 <@t> omega.med.yale.edu Mon Jul 24 17:27:41 2006 From: jdr43 <@t> omega.med.yale.edu (jdr43@omega.med.yale.edu) Date: Mon Jul 24 17:27:46 2006 Subject: [Histonet] pan human cell marker Message-ID: <1153780061.44c5495d83ad5@webmail.med.yale.edu> Hi All- I'm a medical student working on a mouse-human chimera system for studying vascular grafts. Basically, am implanting grafts seeded with mixed populations of human cells into immunodeficient mice. I am currently explanting these grafts at different time points and trying to determine if the cellular infiltrates are human or mouse in origin by immunohistochemistry. Only problem is that I am having difficulty finding an antibody that is a pan-human cell marker. If anyone knows if one exists for immunohistochemistry or a better way of identifying human cells in these grafts, I'd appreciate any and all advice. Thanks so much you guys, Jason Roh Yale Medical Student New Haven, CT 06510 From liz <@t> premierlab.com Mon Jul 24 17:48:01 2006 From: liz <@t> premierlab.com (Elizabeth Chlipala) Date: Mon Jul 24 17:48:18 2006 Subject: [Histonet] pan human cell marker In-Reply-To: <1153780061.44c5495d83ad5@webmail.med.yale.edu> Message-ID: <000201c6af73$37be5c40$0300a8c0@Chlipala> Jason We on occasion work on seeded construct sample that are implanted into several species. We use in-situ hybridization to determine whether or not the cells are from a specific species. For example we have used mouse DNA satilite sequences to determine mouse cells from seeded porcine cells. In that project we used a Mouse satellite DNA repeat sequence that only appears in the Mouse but not the Pig genome. There are several papers that look at in-situ hybridization to determine human cells. I'll attach one in an additional e-mail. We got our probes from GeneDetect and they were very helpful with helping us pick out what we needed. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, Colorado 80308 Office: (303) 735-5001 Fax: (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory University of Colorado MCDB, Room A3B40 Boulder, Colorado 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of jdr43@omega.med.yale.edu Sent: Monday, July 24, 2006 4:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] pan human cell marker Hi All- I'm a medical student working on a mouse-human chimera system for studying vascular grafts. Basically, am implanting grafts seeded with mixed populations of human cells into immunodeficient mice. I am currently explanting these grafts at different time points and trying to determine if the cellular infiltrates are human or mouse in origin by immunohistochemistry. Only problem is that I am having difficulty finding an antibody that is a pan-human cell marker. If anyone knows if one exists for immunohistochemistry or a better way of identifying human cells in these grafts, I'd appreciate any and all advice. Thanks so much you guys, Jason Roh Yale Medical Student New Haven, CT 06510 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Mon Jul 24 18:06:00 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Jul 24 18:06:28 2006 Subject: [Histonet] CAE and Mature Mast cells look at procedure Message-ID: Several comments: Leave the New Fuchsin and 4% Sodium Nitrite solution for 2-3 minutes before adding to the buffer. (The solution should lighten after some bubbling). Stain the sections longer than the 20 minutes. Check microscopically. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Madary, Joseph Sent: Tuesday, 25 July 2006 2:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAE and Mature Mast cells look at procedure Okay my histoheroes and heroines, One of the investigators here asked for a CAE designed for plastics(but supposedly works on FFPE). I did this protocol, but it isn't working. I am having a rough time because I am not used to making mistakes(kidding). She needs mature mast cells only, I gave her 7 different Mast cell procedures that all worked very well(even the dominicis), and one Alcian Blue Safranin that stains Heparin containing Mast cells red Amine containing Mast cells blue. So 2 questions are: 1-Do mature cells stain differently? 2-Does anyone have a decent CAE they can share? Here is the one I used and the control is fresh rat skin and all the other procedures worked on that block. Chloroacetate Esterase (CAE) Staining Preparation of Solutions 1. New Fuchsin Solution New Fuchsin 1g 2N hydrochloric acid (HCl) 25ml 2. 4% Sodium Nitrite Solution Sodium Nitrite 1g ddH2O 25ml 3. Phosphate Buffer (0.1M, pH 7.6) Stock Solutions A. 0.2M solution of monobasic soduim phosphate (27.8g NaH2PO4 in 1L H2O) B. 0.2M solution of dibasic sodium phosphate (28.4g Na2HPO4 in 1L H2O) --> To make pH 7.6 Solution: mix 6.5mL of A, 43.5mL of B, and 50mL of ddH2O. 4. Naphthol AS-D Chloroacetate Solution (Store at -20?C) Naphthol AS-D Chloroacetate 10mg 100mg N-N Dimethyl Formamide 5ml 50ml Staining Solution (make fresh before each use) In a single vial: 1) mix New Fuchsin and 4% Soduim Nitrite 2) add phosphate buffer and mix 3) add Naphth AS-D Chloroacetate Soultion and mix well Use 1 ml freshly mixed solution for approx 4 tissue slides New Fuchsin 2.5?l 5?l 12.5?l 25?l 4% Sodium Nitrite 2.5?l 5?l 12.5?l 25?l Phosphate Buffer 1ml 2ml 5ml 10ml Naphthol AS-D Chloroacetate 50?l 100?l 250?l 500?l Staining Procedure 1. Stain with fresh staining solution for 20 min. 2. Wash with running tap water (For plastic sections use wet cotton swab wipe off dusty residue). 3. Counterstain with Gill's II hematoxylin for 20 to 45 seconds (or methyl green for 30 seconds). 4. Wash with warm tap water to blue (longer will result darker blue, or dip into 50% of lithium carbonate for 5 - 20 seconds for deeper blue, then rinse with water). Dry and mount with mounting medium. (For paraffin sections, quick dehydration before mounting). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From Godsgalnow <@t> aol.com Mon Jul 24 19:55:11 2006 From: Godsgalnow <@t> aol.com (Godsgalnow@aol.com) Date: Mon Jul 24 19:55:20 2006 Subject: [Histonet] gill 2 hematox Message-ID: <2dd.aee6d4f.31f6c5ef@aol.com> Hey out there in histoland..... Anyone using gill 2 for routine h&e staining? Any problems? Willing to share your protocol? Roxanne From nickle_6 <@t> netzero.net Mon Jul 24 21:21:09 2006 From: nickle_6 <@t> netzero.net (nickle_6@netzero.net) Date: Mon Jul 24 21:22:53 2006 Subject: [Histonet] HTL route 1 cert Message-ID: <20060724.192201.3453.403070@webmail32.nyc.untd.com> Hello all, I have been accepted into an HT program Fall '06 and have a BS in biology. How well does this prepare one for HTL boards and what can I expect (or hope for) from the institution I will be attending concerning adequate preparation? Or, is their only obligation at the HT level? Thanks. Regards, Dan Brown From gu.lang <@t> gmx.at Tue Jul 25 01:17:23 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Jul 25 01:17:19 2006 Subject: [Histonet] Dewax for IF In-Reply-To: <44C50201.5090600@tufts.edu> Message-ID: <000901c6afb1$fe355ca0$eeeea8c0@SERVER01> Melissa, We dewax like this: 3x5 min xylene, 100-96-80-50-A.d. each 1 min We do IF on FFPE renal biopsies. Greetings Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Melissa Mazan Gesendet: Montag, 24. Juli 2006 19:23 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: Histonet Digest, Vol 32, Issue 24 Hi all, Can those of you who do non-automated dewaxing share their protocols with me? At first I was doing 3X 3 minutes xylene, then 1X 2 minutes 100% EtOH, then one minute of 85%, and one minute of 75%. Having problems with antigen labeling (immunofluorescence) so am worried that it might be my dewaxing protocol. Also, how often do you completely change out the xylene? Thanks in advance - Melissa histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Gomori's Trichrome on Cementum of a Human Tooth (Ho, Sunita) > 2. Rapid Tissue Processor (a.schmidt@fuse.net) > 3. Re: Fluorophore adsorption emission and spectral separation > (Adam Perry) > 4. RE: Commercial Decalcifying Solns. (patsy ruegg) > 5. IHC validation of blocks and anitbodies (Jessica Piche) > 6. CAE and Mature Mast cells look at procedure (Madary, Joseph) > 7. Postfixation of frozen brain tissue (Robert J. Schloesser) > 8. Can Alk phs be run on peroxidase slides? (Dana Settembre) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sun, 23 Jul 2006 13:54:35 -0700 > From: "Ho, Sunita" > Subject: [Histonet] Gomori's Trichrome on Cementum of a Human Tooth > To: histonet@lists.utsouthwestern.edu > Message-ID: > <0D6FC3C553E12A43B7C6B1F4A9DA429301F60D8B@EXVS05.net.ucsf.edu> > Content-Type: text/plain; charset=us-ascii > > Hi All, > > I recently stained a decalcified human cementum tissue with Gomori's > Trichrome (using a standard procedure provided in any histology text > book). I have regions of red and blue - Cementum was predominantly > stained red and dentin blue (consistently observed between many > specimens). I am assuming that this was primarily due to packing > density of collagen, more in cementum than in dentin. The cementum > around the lacunae was stained blue. Is my assumption correct or is > there a better technical answer for what I observed using a light > microscope? I look forward to your thoughts. > > Thanks in advance. > > Sunita > > > > > ------------------------------ > > Message: 2 > Date: Sun, 23 Jul 2006 19:46:32 -0400 > From: > Subject: [Histonet] Rapid Tissue Processor > To: Histonet@lists.utsouthwestern.edu > Message-ID: <22333582.1153698392694.JavaMail.root@webmail6> > Content-Type: text/plain; charset=utf-8 > > We have been using the Sakura Rapid Tissue processor for about 3 months now. We have been having trouble with our digested PAS stain on liver needle biopsies using the Ventana Nexes stainer. Has any other facility experinced any problems with their special stains or IHC stains and if you have this processor I would appreciate hearing any of your feelings about it. Tahkns for the input. > Angie Schmidt > Tri-Health > Labs--Cincinnati > > > > ------------------------------ > > Message: 3 > Date: Mon, 24 Jul 2006 07:47:45 -0700 (PDT) > From: Adam Perry > Subject: Re: [Histonet] Fluorophore adsorption emission and spectral > separation > To: Histonet@lists.utsouthwestern.edu > Message-ID: <20060724144745.41330.qmail@web30401.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > I had an immunofluorescence question that I'd love to get some input on from anyone out there. I'm trying to colocalize two antigens in rodent brain sections. One of the antigens is yielding fairly weak staining- it also happens to be the one that I'm visualizing with Alexa Fluor 488, so I'm getting fairly high background autofluorescence- and I think that the staining could be improved by reducing the autofluorescence. My DAPI staining (ex/em: 350/470) is great...it only stains nuclei with minimal autofluorescence. They offer Alexa Fluor 405 streptavidin which has a similar excit/emission range, so I thought labeling with the Alexa Fluor 405 would produce a similarly "clean" background as my DAPI labeling. Also, I already have the filter sets to work with Alexa Fluor 405 (as opposed to going to the other end of the spectrum). > > Has anyone used Alexa Fluor 405? What is your impression of tissue autofluorescence in that range, photostability/bleaching of the compounds, comparison of signal using other fluorophores, etc. > > Any input would be greatly appreciated. > Thanks, > Adam Perry > > Department of Neuroscience > University of Illinois at Chicago > Chicago, IL 60612 > > > Gayle Callis wrote: > Moran, > > Molecular Probes, go to resources on home page for MP, and click on > their spectraimager. You can play with the fluorophores you need to > see (do it as antibodies) and see the graphs for separation - for both > CLSM and fluorescent microscope work. > > With our confocal, adjustment can be made to eliminate > autofluorescence. I also have a review article on autofluorescence, > written with GFP in mind, but useful for immunofluorescent work also. > > If you want the review, I will send via private email. > > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 > 406 994-4303 (FAX) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Do you Yahoo!? > Get on board. You're invited to try the new Yahoo! Mail Beta. > > ------------------------------ > > Message: 4 > Date: Mon, 24 Jul 2006 09:19:49 -0600 > From: "patsy ruegg" > Subject: RE: [Histonet] Commercial Decalcifying Solns. > To: "'Marilyn Johnson'" , > > Message-ID: <200607241519.k6OFJhlG059684@pro12.abac.com> > Content-Type: text/plain; charset="US-ASCII" > > I prefer Immunocal by Decal Chemicals, it seems to be just as fast as > the more harsh HCL based decals like RDO but much much better for IHC, > it is formic acid based. > Patsy > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. #216 > Aurora, CO 80010 > 720-859-4060 > fax 720-859-4110 > pruegg@ihctech.net > www.ihctech.net > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > Marilyn Johnson > Sent: Saturday, July 22, 2006 8:47 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Commercial Decalcifying Solns. > > Hi Histonetters, > Could anyone provide me with their preferences for commercially > prepared decalcifying solns? > These solns will be required for routine bone sections for H&E > staining and not biopsies or rush specimens. > Please provide company names and order numbers. > Thank you in advance for any replies. > > Marilyn Johnson > Alberta Agriculture > Edmonton, AB. Canada. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 5 > Date: Mon, 24 Jul 2006 09:17:45 -0700 (PDT) > From: Jessica Piche > Subject: [Histonet] IHC validation of blocks and anitbodies > To: histonet > Message-ID: <20060724161745.56186.qmail@web82011.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hi All, > > I have a few questions regarding IHC. When validating controls and new antibodies do you always run a negative control slide as well? Can anyone share their protocols with me on validating controls, and new antibodies? Any help would be appreciated. Thank you. > > Jessica Piche-Grocki, HT (ASCP) > > > ------------------------------ > > Message: 6 > Date: Mon, 24 Jul 2006 12:22:19 -0400 > From: "Madary, Joseph" > Subject: [Histonet] CAE and Mature Mast cells look at procedure > To: > Message-ID: > <7CAB706201F11843BD26AD516326F0C8010D3890@MD1MS007.medimmune.com> > Content-Type: text/plain; charset="iso-8859-1" > > Okay my histoheroes and heroines, > One of the investigators here asked for a CAE designed for plastics(but supposedly works on FFPE). I did this protocol, but it isn't working. I am having a rough time because I am not used to making mistakes(kidding). She needs mature mast cells only, I gave her 7 different Mast cell procedures that all worked very well(even the dominicis), and one Alcian Blue Safranin that stains Heparin containing Mast cells red Amine containing Mast cells blue. So 2 questions are: > > 1-Do mature cells stain differently? > 2-Does anyone have a decent CAE they can share? Here is the one I used and the control is fresh rat skin and all the other procedures worked on that block. > > Chloroacetate Esterase (CAE) Staining > > Preparation of Solutions > > 1. New Fuchsin Solution > New Fuchsin 1g > 2N hydrochloric acid (HCl) 25ml > > 2. 4% Sodium Nitrite Solution > Sodium Nitrite 1g > ddH2O 25ml > > 3. Phosphate Buffer (0.1M, pH 7.6) > Stock Solutions > A. 0.2M solution of monobasic soduim phosphate (27.8g NaH2PO4 in 1L H2O) > B. 0.2M solution of dibasic sodium phosphate (28.4g Na2HPO4 in 1L H2O) > --> To make pH 7.6 Solution: mix 6.5mL of A, 43.5mL of B, and 50mL of ddH2O. > > 4. Naphthol AS-D Chloroacetate Solution (Store at -20?C) > Naphthol AS-D Chloroacetate 10mg 100mg > N-N Dimethyl Formamide 5ml 50ml > > > Staining Solution (make fresh before each use) > > In a single vial: 1) mix New Fuchsin and 4% Soduim Nitrite > 2) add phosphate buffer and mix > 3) add Naphth AS-D Chloroacetate Soultion and mix well > > Use 1 ml freshly mixed solution for approx 4 tissue slides > New Fuchsin 2.5?l 5?l 12.5?l 25?l > 4% Sodium Nitrite 2.5?l 5?l 12.5?l 25?l > Phosphate Buffer 1ml 2ml 5ml 10ml > Naphthol AS-D Chloroacetate 50?l 100?l 250?l 500?l > > Staining Procedure > 1. Stain with fresh staining solution for 20 min. > 2. Wash with running tap water (For plastic sections use wet cotton swab wipe off dusty residue). > 3. Counterstain with Gill's II hematoxylin for 20 to 45 seconds (or methyl green for 30 seconds). > 4. Wash with warm tap water to blue (longer will result darker blue, or dip into 50% of lithium carbonate for 5 - 20 seconds for deeper blue, then rinse with water). > Dry and mount with mounting medium. (For paraffin sections, quick dehydration before mounting). > > > > ------------------------------ > > Message: 7 > Date: Mon, 24 Jul 2006 12:41:50 -0400 > From: "Robert J. Schloesser" > Subject: [Histonet] Postfixation of frozen brain tissue > To: > Message-ID: <000001c6af40$0ff4ff80$76e4bb89@yourd54swmkxh6> > Content-Type: text/plain; charset="iso-8859-1" > > Hi All, > > We have to do IHC on fresh frozen brain samples perfused only with > saline. I am only used to cutting and IHC on PFA fixed brains. > Therefore I have many questions on how to treat fresh frozen samples. > > Postfixation on slides: PFA or Acetone/Ethanol? > Is there a reliable way to postfix whole blocks? > Is there a way to postfix 30-200?m thick slides without mounting them > on slide (to do free floating staingin)? > > Any kind of input, protocols or tips&tricks and do`s and don`t are > highly appreciated. > > Thank you very much > > Robert > > > > > ------------------------------ > > Message: 8 > Date: Mon, 24 Jul 2006 12:44:48 -0400 > From: "Dana Settembre" > Subject: [Histonet] Can Alk phs be run on peroxidase slides? > To: > Message-ID: > Content-Type: text/plain; charset=US-ASCII > > Hi, > One of my pathologists is asking if I can take a slide that has > already been stained with a peroxidase/avidin/biotin/DAB detection > system and re-stain with an Alkaline Phosphotase detection system. > Can it work? > Must it be de-stained? > > Any info will help. Thanks, > > Dana Settembre > Immunohistochemistry Lab > University Hospital - UMDNJ > Newark, NJ > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 32, Issue 24 > **************************************** -- Melissa R. Mazan, DVM, Diplomate ACVIM Associate Professor of Large Animal Medicine Director of Sports Medicine Cummings School of Veterinary Medicine Tufts University 200 Westborough Road North Grafton, MA 01536 Tel: 508-839-5395 Fax: 508-839-7922 Email: melissa.mazan@tufts.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Tue Jul 25 01:40:28 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Jul 25 01:40:24 2006 Subject: AW: [Histonet] CAE and Mature Mast cells look at procedure In-Reply-To: <7CAB706201F11843BD26AD516326F0C8010D3890@MD1MS007.medimmune.com> Message-ID: <000a01c6afb5$37a88450$eeeea8c0@SERVER01> We use the same procedure, but instead of phosphate buffer we take veronalbuffer. In summer, in our old lab, we often had problems with this stain. We thought it was because of the "summer-heat". Now in the klimated new lab, it works constantly. I always shake the fuchsin-natriumnitrit-solution rather rigid, until the "chloro"-smell disappears. After adding the buffer, the solution must be yellowish. With a red tinge, it doesn't work. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Madary, Joseph Gesendet: Montag, 24. Juli 2006 18:22 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] CAE and Mature Mast cells look at procedure Okay my histoheroes and heroines, One of the investigators here asked for a CAE designed for plastics(but supposedly works on FFPE). I did this protocol, but it isn't working. I am having a rough time because I am not used to making mistakes(kidding). She needs mature mast cells only, I gave her 7 different Mast cell procedures that all worked very well(even the dominicis), and one Alcian Blue Safranin that stains Heparin containing Mast cells red Amine containing Mast cells blue. So 2 questions are: 1-Do mature cells stain differently? 2-Does anyone have a decent CAE they can share? Here is the one I used and the control is fresh rat skin and all the other procedures worked on that block. Chloroacetate Esterase (CAE) Staining Preparation of Solutions 1. New Fuchsin Solution New Fuchsin 1g 2N hydrochloric acid (HCl) 25ml 2. 4% Sodium Nitrite Solution Sodium Nitrite 1g ddH2O 25ml 3. Phosphate Buffer (0.1M, pH 7.6) Stock Solutions A. 0.2M solution of monobasic soduim phosphate (27.8g NaH2PO4 in 1L H2O) B. 0.2M solution of dibasic sodium phosphate (28.4g Na2HPO4 in 1L H2O) --> To make pH 7.6 Solution: mix 6.5mL of A, 43.5mL of B, and 50mL of ddH2O. 4. Naphthol AS-D Chloroacetate Solution (Store at -20?C) Naphthol AS-D Chloroacetate 10mg 100mg N-N Dimethyl Formamide 5ml 50ml Staining Solution (make fresh before each use) In a single vial: 1) mix New Fuchsin and 4% Soduim Nitrite 2) add phosphate buffer and mix 3) add Naphth AS-D Chloroacetate Soultion and mix well Use 1 ml freshly mixed solution for approx 4 tissue slides New Fuchsin 2.5?l 5?l 12.5?l 25?l 4% Sodium Nitrite 2.5?l 5?l 12.5?l 25?l Phosphate Buffer 1ml 2ml 5ml 10ml Naphthol AS-D Chloroacetate 50?l 100?l 250?l 500?l Staining Procedure 1. Stain with fresh staining solution for 20 min. 2. Wash with running tap water (For plastic sections use wet cotton swab wipe off dusty residue). 3. Counterstain with Gill's II hematoxylin for 20 to 45 seconds (or methyl green for 30 seconds). 4. Wash with warm tap water to blue (longer will result darker blue, or dip into 50% of lithium carbonate for 5 - 20 seconds for deeper blue, then rinse with water). Dry and mount with mounting medium. (For paraffin sections, quick dehydration before mounting). _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.m.vanderloos <@t> amc.uva.nl Tue Jul 25 01:50:29 2006 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Tue Jul 25 01:50:41 2006 Subject: [Histonet] RE: Can Alk phs be run on peroxidase slides? Message-ID: Dana, Basically I think this is possible. In the end it's a kind of sequential double staining. Some remarks: * Of course you have to be aware of animal species: if your AP stain is another mouse primary you may end up with a cross-reactions. This type of cross-reaction is not really expected since DAB was used as chromogen. DAB reaction product has the unique characteristic of effectively 'sheltering' all immunoreagents used in the first staining sequence. * If you want to be on the safe side with cross-reactions, perform HIER (using the same buffer as for the first one) for just 5 min + 10 min cooldown. This effectively removes or at least destroy all your first step reagents while the DAB reaction product remains unaffected. * A second streptavidin-biotin detection system is not recommended. To be on the safe side use a AP-labeled polymer. In case you really need another streptavidin-biotin detection system, perform a biotin blocking first (avidin 0.1%, 15 min - d-biotin 0.01%, 15 min, or a commercial biotin blocking kit). * Given for example that your first DAB stain is cytoplasmic and the second AP stain is nuclear, AP in red with Liquid Permanent Red (Dako) is a good choice. * Destaining of a DAB-stained slide will be a very hard job, not to say impossible. Lots of success, Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Mon, 24 Jul 2006 12:44:48 -0400 From: "Dana Settembre" Subject: [Histonet] Can Alk phs be run on peroxidase slides? To: Hi, One of my pathologists is asking if I can take a slide that has already been stained with a peroxidase/avidin/biotin/DAB detection system and re-stain with an Alkaline Phosphotase detection system. Can it work? Must it be de-stained? Any info will help. Thanks, Dana Settembre Immunohistochemistry Lab University Hospital - UMDNJ Newark, NJ From narangakhil <@t> yahoo.com Tue Jul 25 02:07:41 2006 From: narangakhil <@t> yahoo.com (Akhil Narang) Date: Tue Jul 25 02:07:45 2006 Subject: [Histonet] Immunostaining VEGF Message-ID: <20060725070741.64643.qmail@web50105.mail.yahoo.com> Hello everyone, Recently subscribed to the list and I would love any input. I am looking to stain a cell line for the presence of VEGF and I was wondering if anyone has any protocols or suggestions as to how to go about this. Is there any way to stain a cell culture without having to fix the cells onto a slide or grow the cells on a slide? If so, any ideas? Also, what antibody/secondary antibody have people had success with? Concentrations/dilutions? Thanks for your help. Akhil __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From kemlo.rogerson <@t> waht.swest.nhs.uk Tue Jul 25 02:08:55 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Jul 25 02:08:25 2006 Subject: [Histonet] gill 2 hematox Message-ID: Used Gill 2 when I was a pup; 5 mins for freshly made Gill 2, sometimes very little differentiation needed until it got old. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 I am a part of all that I have met. --Alfred Tennyson This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Terry.Marshall <@t> rothgen.nhs.uk Tue Jul 25 06:57:07 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Tue Jul 25 06:55:17 2006 Subject: [Histonet] gill 2 hematox Message-ID: What made you change (from Gill's of course, rather than from being a pup)? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk -----Original Message----- From: Kemlo Rogerson [mailto:kemlo.rogerson@waht.swest.nhs.uk] Sent: 25 July 2006 08:09 To: 'Godsgalnow@aol.com'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] gill 2 hematox Used Gill 2 when I was a pup; 5 mins for freshly made Gill 2, sometimes very little differentiation needed until it got old. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 I am a part of all that I have met. --Alfred Tennyson This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From arvind <@t> nbrc.res.in Tue Jul 25 07:12:05 2006 From: arvind <@t> nbrc.res.in (Arvind Pundir) Date: Tue Jul 25 07:07:00 2006 Subject: [Histonet] querry References: Message-ID: Hello Everyone, I am trying to standardize slice culture for bird brain the sections are 400 um thick , then in between incorporated Brdu to the media. can any one please brief the detailed protocol for the slice culture in detail thanking in advance Arvind Singh Pundir National Brain Research Centre, Manesar, Gurgaon, Haryana, India From doug <@t> ppspath.com Tue Jul 25 07:14:49 2006 From: doug <@t> ppspath.com (Douglas D. Deltour) Date: Tue Jul 25 07:11:53 2006 Subject: [Histonet] Sakura Rack Adapter for Leica Message-ID: Hello, I am looking for the metal rack adapters that fit on the Sakura slide baskets/rack so I can run them on a Leica stainer. Thanks! Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. From TillRenee <@t> uams.edu Tue Jul 25 07:16:13 2006 From: TillRenee <@t> uams.edu (Till, Renee) Date: Tue Jul 25 07:16:41 2006 Subject: [Histonet] plastic cryo molds Message-ID: <11F927674DEBDC43B960809A7403C5D20183EDF6@MAILPED.ad.uams.edu> Does anyone know if they make any of the plastic cryo molds deeper than the standard ones? I know someone who needs to freeze a whole rat heart from rats 3-5 months old. Or does any one have any ideas what could be used ? Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72002 Office (501)364-2785 Fax (501)364-3161 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From JurciseJ <@t> pediatrics.ohio-state.edu Tue Jul 25 07:28:53 2006 From: JurciseJ <@t> pediatrics.ohio-state.edu (Jurcisek, Joseph) Date: Tue Jul 25 07:30:41 2006 Subject: [Histonet] paraformaldehyde Message-ID: <714B9F12B4E18C4C843B66E8E190F2ADB1EE29@res2k3ms01.CRII.ORG> Here is the protocol that I have used for years. I have never had to adjust the pH after making the paraformaldehyde and the procedure is very straightforward. I usually make 2% paraformaldehyde for my use, but I have made 4% for others (following the same protocol) and they have used it in their immuno protocol with very good results. Note, the final concentration of the phosphate buffer is 0.1M. 0.2M phosphate buffer (stock) A. monobasic NaHPO4 (fw 137.99) 2.78 gm diH2O 100 ml B. dibasic Na2HPO4 (fw 141.96) 28.4 gm diH2O 1000 ml mix 60 ml (A) and 920 ml (B). Adjust pH to 7.4 store @ 4 C. (very little adjustment should be needed here based on these volumes) 2% paraformaldehyde A. 0.2M phosphate buffer 500 ml B. diH2O 500 ml C. EM grade paraformaldehyde 20 gm mix (A), (B), and (C), stir while heating to 60 C. If remains cloudy, adjust pH to 7.4 with 1N NaOH (I have never had to do this). Store at 4 C. To make 4% just double the amount of paraformaldehyde. Joseph A. Jurcisek Senior Research Associate Center for Microbial Pathogenesis Columbus Children's Research Institute 700 Children's Dr. Columbus, OH 43205-2696 (614) 355-3521 fax (614) 722-2818 jurcisej@ccri.net -----Original Message----- From: Hawkins, Hal K. [mailto:hhawkins@utmb.edu] Sent: Monday, July 24, 2006 3:44 PM To: histonet Subject: [Histonet] paraformaldehyde We need to prepare 4% phosphate-buffered paraformaldehyde for a cooperative study in fairly large volumes, about 2 L at a time. My only experience in preparing this fixative involved heating to over 60 C, adding sodium hydroxide chips until all was dissolved, then adding the buffer and adjusting the pH. There seems to be a simpler method in which pre-mixed Dulbecco PBS is warmed and the paraformaldehyde is dissolved in the buffer. Has anyone used this method and does it work without further adjustment of the final pH? Thanks in advance, Hal Hawkins UTMB, Galveston, Texas From kemlo.rogerson <@t> waht.swest.nhs.uk Tue Jul 25 07:48:04 2006 From: kemlo.rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Jul 25 07:47:30 2006 Subject: [Histonet] gill 2 hematox Message-ID: Different job, no staining needed. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 I am a part of all that I have met. --Alfred Tennyson This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From POWELL_SA <@t> Mercer.edu Tue Jul 25 08:11:12 2006 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Tue Jul 25 08:11:48 2006 Subject: [Histonet] plastic cryo molds In-Reply-To: <11F927674DEBDC43B960809A7403C5D20183EDF6@MAILPED.ad.uams.edu> Message-ID: <01M578MU29FK8X1V89@Macon2.Mercer.edu> There are some molds used for plastic embedding that are deeper that may work. Try Polysciences, EM Sciences or one of those companies that provide products for the plastics. Shirley -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Till, Renee Sent: Tuesday, July 25, 2006 7:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] plastic cryo molds Does anyone know if they make any of the plastic cryo molds deeper than the standard ones? I know someone who needs to freeze a whole rat heart from rats 3-5 months old. Or does any one have any ideas what could be used ? Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72002 Office (501)364-2785 Fax (501)364-3161 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mari.ann.mailhiot <@t> leica-microsystems.com Tue Jul 25 08:56:45 2006 From: mari.ann.mailhiot <@t> leica-microsystems.com (mari.ann.mailhiot@leica-microsystems.com) Date: Tue Jul 25 09:08:14 2006 Subject: [Histonet] Sakura Rack Adapter for Leica In-Reply-To: <20060725121217.62503D00F854@de-barracuda.leica-microsystems.com> Message-ID: Douglas Please contact me here at Leica. I need to know what Leica stainer you have. Regards Mari Ann Mailhiot BA HT ASCP Application Specialist Leica Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailhiot@leica-microsystems.com www.leica-microsystems.com "Douglas D. Deltour" Sent by: cc histonet-bounces@ lists.utsouthwest Subject ern.edu [Histonet] Sakura Rack Adapter for Leica 07/25/2006 07:14 AM Hello, I am looking for the metal rack adapters that fit on the Sakura slide baskets/rack so I can run them on a Leica stainer. Thanks! Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ From algranth <@t> u.arizona.edu Tue Jul 25 09:24:17 2006 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Tue Jul 25 09:25:29 2006 Subject: [Histonet] plastic cryo molds In-Reply-To: <01M578MU29FK8X1V89@Macon2.Mercer.edu> References: <11F927674DEBDC43B960809A7403C5D20183EDF6@MAILPED.ad.uams.edu> Message-ID: <4.3.2.7.2.20060725071745.00cea260@algranth.inbox.email.arizona.edu> I saw these at NSH 2 yrs ago and recently purchased some from BBC Biochemical. Call Adrian at 800-635-4477. I use them for exactly the same reason and they are great! Only problem is that they only have the larger size. Not sure but I think Simport makes them - they need to know how great the deeper mold is and that a smaller size would be nice - like the 15x15mm or the 7x7mm. Andi Grantham At 09:11 AM 7/25/2006 -0400, Shirley Powell wrote: >There are some molds used for plastic embedding that are deeper that may >work. Try Polysciences, EM Sciences or one of those companies that provide >products for the plastics. >Shirley > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Till, Renee >Sent: Tuesday, July 25, 2006 7:16 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] plastic cryo molds > >Does anyone know if they make any of the plastic cryo molds deeper than the >standard ones? I know someone who needs to freeze a whole rat heart from >rats 3-5 months old. Or does any one have any ideas what could be used ? > > > >Renee' Till, HT > >Research Assistant > >Arkansas Children's Nutrition Center > >1212 Marshall St./N2021 > >Little Rock, AR 72002 > >Office (501)364-2785 > >Fax (501)364-3161 > > > > >Confidentiality Notice: This e-mail message, including any attachments, is >for the sole use of the intended recipient(s) and may contain confidential >and privileged information. Any unauthorized review, use, disclosure or >distribution is prohibited. If you are not the intended recipient, please >contact the sender by reply e-mail and destroy all copies of the original >message. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From pedro.louro <@t> spcorp.com Tue Jul 25 09:44:31 2006 From: pedro.louro <@t> spcorp.com (Louro, Pedro) Date: Tue Jul 25 09:45:47 2006 Subject: [Histonet] Nitrotyrosine control Message-ID: Hi everyone, I'm looking for paraffin / frozen positive tissue control blocks or slides for the detection of Nitrotyrosine. Does anyone have any info. on where I can get search for this. Thanks in advance, Pedro Louro Schering-Plough Research Institute ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From anh2006 <@t> med.cornell.edu Tue Jul 25 09:45:51 2006 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Tue Jul 25 09:47:10 2006 Subject: [Histonet] plastic cryo molds In-Reply-To: <4.3.2.7.2.20060725071745.00cea260@algranth.inbox.email.arizona.edu> References: <11F927674DEBDC43B960809A7403C5D20183EDF6@MAILPED.ad.uams.edu> <4.3.2.7.2.20060725071745.00cea260@algranth.inbox.email.arizona.edu> Message-ID: We use peel-a-way molds for freezing large size tissues in OCT for cryosectioning. From VWR. They sell them in several dimensions and will definitely be large enough. Here is the link: http://www.vwrsp.com/catalog/product/index.cgi?catalog_number=15160-270&inE=1&highlight=15160-270 >> >>-----Original Message----- >>From: histonet-bounces@lists.utsouthwestern.edu >>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Till, Renee >>Sent: Tuesday, July 25, 2006 7:16 AM >>To: histonet@lists.utsouthwestern.edu >>Subject: [Histonet] plastic cryo molds >> >>Does anyone know if they make any of the plastic cryo molds deeper than the >>standard ones? I know someone who needs to freeze a whole rat heart from >>rats 3-5 months old. Or does any one have any ideas what could be used ? -- From abright <@t> brightinstruments.com Tue Jul 25 10:18:27 2006 From: abright <@t> brightinstruments.com (Alan Bright) Date: Tue Jul 25 10:08:08 2006 Subject: [Histonet] plastic cryo molds Message-ID: Dear Renee, I see that you have had a few replies, if these sizes do not suit your study, then perhaps you can let me know what sizes you require and the quantities. This will then enable me inform you what we can offer. Also for cryo moulds I have been finding that metal ones are far superior to plastic as the front sectioning face stays flat, they freeze much faster and they are much easier to release from the mould. I will leave this with you if you are interested. Best Regards Alan Bright Bright Instrument Co.Ltd. St Margaret's Way Huntingdon Cambridgeshire PE29 6EU England Tel No:+44 (0)1480 454528 Fax No:+44 (0)1480 456031 Email: abright@brightinstruments.com Web Site: www.brightinstruments.com Skype User ID: dazzle0 -----Original Message----- From: Till, Renee [mailto:TillRenee@uams.edu] Sent: 25 July 2006 13:16 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] plastic cryo molds Does anyone know if they make any of the plastic cryo molds deeper than the standard ones? I know someone who needs to freeze a whole rat heart from rats 3-5 months old. Or does any one have any ideas what could be used ? Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72002 Office (501)364-2785 Fax (501)364-3161 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Amanda.Garcia <@t> TriadHospitals.com Tue Jul 25 10:34:30 2006 From: Amanda.Garcia <@t> TriadHospitals.com (Garcia, Amanda) Date: Tue Jul 25 10:54:23 2006 Subject: [Histonet] Releasing specimens to patients Message-ID: <8B63039C9DF4554C8FDBF31235F0E14801B8E536@CPRTEVS02.triadhospitals.net> Hello everyone and thanks in advance for any input you all may have to this question. Do any of you have a policy regarding the release of fetuses to the family? We have a 12 week fetus that the family would like to pick up and this hospital does not have a policy regarding this issue. At a previous private pathology lab that I worked at we have a policy stating that we could only release this to a funeral home. If anyone has a policy and wouldn't mind sharing it with me it would be greatly appreciated. Thanks again, Amy > Amanda (Amy) Garcia > Histology/Pathology > College Station Medical Center > (979) 680-5372 office > (979) 696-5446 fax > *Mailto:amanda.garcia@triadhospitals.com > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the e-mail or any > of its attachments, please be advised that you have received this > e-mail in error and that any use, dissemination, distribution, > forwarding, printing, or copying of this e-mail or any attached files > is strictly prohibited. If you have received this e-mail in error, > please immediately purge it and all attachments and notify the sender > by reply e-mail or contact the sender at the number listed. > > > From n.cragg <@t> epistem.co.uk Tue Jul 25 11:10:19 2006 From: n.cragg <@t> epistem.co.uk (Nicola Cragg) Date: Tue Jul 25 11:12:18 2006 Subject: [Histonet] Distinguishing human cells in grafts Message-ID: Hi Jason & Histonetters, We use Hoechst stain (33258) to distinguish between human and mouse cells in grafts. It's really quick & easy, just dewax, rehydrate & incubate in Hoechst at 4ug/ml for 1 minute, rinse with runnig water for 5 minutes, coverslip with an anti-fade mountant & look under a fluroescene microscope. Mouse cells show discrete intranuclear fluorescent bodies, i.e. they appear "spotty"/ punctate / grainy, but human cells don't, i.e they appear plain. Hope this helps, Nic Manchester, UK This message has been scanned for viruses by BlackSpider MailControl - www.blackspider.com From Charles.Embrey <@t> carle.com Tue Jul 25 11:33:57 2006 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Tue Jul 25 11:34:01 2006 Subject: [Histonet] Releasing specimens to patients Message-ID: Ours are ONLY released to a funeral home. Chuck -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Garcia, Amanda Sent: Tuesday, July 25, 2006 10:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Releasing specimens to patients Hello everyone and thanks in advance for any input you all may have to this question. Do any of you have a policy regarding the release of fetuses to the family? We have a 12 week fetus that the family would like to pick up and this hospital does not have a policy regarding this issue. At a previous private pathology lab that I worked at we have a policy stating that we could only release this to a funeral home. If anyone has a policy and wouldn't mind sharing it with me it would be greatly appreciated. Thanks again, Amy > Amanda (Amy) Garcia > Histology/Pathology > College Station Medical Center > (979) 680-5372 office > (979) 696-5446 fax > *Mailto:amanda.garcia@triadhospitals.com > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the e-mail or any > of its attachments, please be advised that you have received this > e-mail in error and that any use, dissemination, distribution, > forwarding, printing, or copying of this e-mail or any attached files > is strictly prohibited. If you have received this e-mail in error, > please immediately purge it and all attachments and notify the sender > by reply e-mail or contact the sender at the number listed. > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From juan.gutierrez <@t> christushealth.org Tue Jul 25 12:11:39 2006 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Tue Jul 25 12:11:49 2006 Subject: [Histonet] Releasing specimens to patients Message-ID: Here in Texas the law requires that all human tissue to be buried, cremated ,etc. are to be released to a licensed funeral home. Only teeth and in-vitro cultures can be released directly to patients. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Garcia, Amanda Sent: Tuesday, July 25, 2006 10:35 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Releasing specimens to patients Hello everyone and thanks in advance for any input you all may have to this question. Do any of you have a policy regarding the release of fetuses to the family? We have a 12 week fetus that the family would like to pick up and this hospital does not have a policy regarding this issue. At a previous private pathology lab that I worked at we have a policy stating that we could only release this to a funeral home. If anyone has a policy and wouldn't mind sharing it with me it would be greatly appreciated. Thanks again, Amy > Amanda (Amy) Garcia > Histology/Pathology > College Station Medical Center > (979) 680-5372 office > (979) 696-5446 fax > *Mailto:amanda.garcia@triadhospitals.com > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the e-mail or any > of its attachments, please be advised that you have received this > e-mail in error and that any use, dissemination, distribution, > forwarding, printing, or copying of this e-mail or any attached files > is strictly prohibited. If you have received this e-mail in error, > please immediately purge it and all attachments and notify the sender > by reply e-mail or contact the sender at the number listed. > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmarcum <@t> vet.upenn.edu Tue Jul 25 12:28:13 2006 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Tue Jul 25 12:28:21 2006 Subject: [Histonet] Pennsylvania Histotechnology Society Fall Seminars Message-ID: <6.1.1.1.2.20060725131336.019ed9f8@mail.vet.upenn.edu> The following is the program listing for our meeting in October, 2006. We have attempted to put together a program that will allow you to get the required CEUs for your certification, get information with a class to complete testing for your certification or just fun with forensics or bog people (mummies). Come join us in Cranbury PA. Just outside Pittsburgh for 1 or all 3 days. If you would like further information call either Gloria Limetti at UPMC 412-647-8532 or Pam Marcum at UPENN Vet School 610-925-6278. The web site will be up later this week with the full program and abstracts. An e-mail will be sent by Friday with the address. Thursday October 26 ? Special Management Seminars This day is designed for Supervisors and Managers to assist in some of the special areas we are all asked to become experts in today with limited resources and opportunities to attend local educational events. If you are a new manager or just need a refresher with updates join us!! WS# 1 8:00AM to 11:30AM CPT Coding - Which Code Do I Use? Whitaker WS# 2 1:00PM to 4:30PM Preparing for a CAP Inspection Nocito & Hernandez WS# 3 4:30PM to 6:00PM The SWEET Workshop Smart Working Environment Ergonomics Training Minshew Friday Oct 27 - Morning Sessions WS# 4 8:00 ? 9:30 AM Richmond Introduction to Fine Needle Aspiration for the Histotechnologist WS# 5 10:00 ? 11:30 AM Histology Laboratory Workload Measurement: Evaluating Complexity and Productivity Schmitt WS# 6 8:00 ? 11:30 AM Don?t Let Immunos Intimidate You! Whitaker Beginning Immunos and Refresher WS# 7 8:00 ? 9:30 AM Safety a Priority in Our Lives Casey WS# 8 10:00 ? 11:30 AM Recycling for Histology Welch WS# 9 8:00AM ? 11:30AM Praet Unlocking the Secrets of Mohs? Grossing and Cryosectioning Friday October 27 ? Afternoon Sessions WS#10 1:00 ? 2:30 PM Detection and Amplification of Nucleic Acids in Morphologically Preserved Cells and Tissues Gore WS# 11 3:00 ? 4:30 PM Smart Shopping and Contracts for Histology Macrea WS # 12 1:00 ? 2:30PM Automated Stains Workshop with Multiple Vendors (15 minutes each) WS# 13 3:00 ? 4:30 PM The Bog People of Northern Europe Olsen WS#14 1:00 ? 4:30 PM Nocito & Grossing Surgical Specimens: A Histologist View Hernandez WS# 15 1:00 ? 4:30 PM Peters Frozen Section Techniques New Methods for Cryo-Embedding Saturday October 28 - Morning Sessions WS#16 8:00 - 11:30 AM Molecular Pathology Haas WS#17 8:00 - 11:30 AM Meyers Wet Workshop:Multi-antigen Immunohistochemistry Staining WS#18 8:00 - 11:30 AM The Art of Forensic Autopsy Mancuso WS #19 8:00AM - 4:30AM QIHC Readiness and Troubleshooting Macrea WS# 20 8:00AM - 4:30PM HT (ASCP) Examination Readiness Micciche Saturday October 28 - Afternoon Sessions WS# 21 1:00 - 2:30 PM What Plastic Do I Embed This Project In? Marcum WS# 22 1:00 - 2:30 PM Specimen Labeling and Tracking TBA WS#23 1:00 ? 2:30 PM Forensic Insect Entomology Todaro Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From mgdelaware <@t> comcast.net Tue Jul 25 12:38:03 2006 From: mgdelaware <@t> comcast.net (Marian Powers) Date: Tue Jul 25 12:38:07 2006 Subject: [Histonet] Colponin Message-ID: <000a01c6b011$15503b30$0711c847@D7XQNX91> Hi: Anyone using colponin? If so, where do I find it? Is it worth having, we presently use P63 and CD10. Thanks in advance, Marian From bliven.laura <@t> marshfieldclinic.org Tue Jul 25 12:45:13 2006 From: bliven.laura <@t> marshfieldclinic.org (bliven.laura@marshfieldclinic.org) Date: Tue Jul 25 12:44:34 2006 Subject: [Histonet] Implications of Various Fixatives Message-ID: <5a6e501c6b012$14e43d80$6b05010a@mfldclinframe.org> Does anyone know of a good published reference addressing various fixatives and their reactions on certain immuno's, special stains and artifacts? I understand that there is a chance that tissue fixed in Bouin and Hollande fixatives can cause false-negative immuno results in some lymphoma markers and found another mention of a certain fixative causing a false-negative result in the Steiner and Steiner. Also it would be great to see the pH of all the fixatives along with information such as "Do not allow Hollande's fixed specimens to come in contact with phosphate buffered formalin, a blue precipitate (artifact) occurs" - and is this what some articles refer to as being "incompatible with NBF"? Thanks much, Laura Laura Bliven Histology Lab/IHC Marshfield Laboratories 1000 N. Oak Ave. Marshfield, WI 54449 From tkngflght <@t> yahoo.com Tue Jul 25 12:48:41 2006 From: tkngflght <@t> yahoo.com (Cheryl) Date: Tue Jul 25 12:48:46 2006 Subject: [Histonet] Releasing specimens to patients In-Reply-To: <8B63039C9DF4554C8FDBF31235F0E14801B8E536@CPRTEVS02.triadhospitals.net> Message-ID: <20060725174841.66159.qmail@web50904.mail.yahoo.com> Hi Amy- The 500 gram weight comes into play in some states, but you're in Texas (see other posting). The easiest way to facilitate this is to treat this like any deceased patient and go through channels already in place. The family has the right to claim the baby, but not the right to step over the laws of the state. Since you have policies and contacts in place for full-sized decedents, just follow the policies and let the people who normally handle this take it back (nursing and pastoral care) to work with the family. We're not trained to be in direct contact with grieving parents and it might open liabilities for your hospital. Most funeral homes are set up to handle this and it's not expensive. They cannot bury a baby in the back yard...and there are other choices. Let the professionals guide them (funeral directors) "Garcia, Amanda" wrote: Hello everyone and thanks in advance for any input you all may have to this question. Do any of you have a policy regarding the release of fetuses to the family? We have a 12 week fetus that the family would like to pick up and this hospital does not have a policy regarding this issue. At a previous private pathology lab that I worked at we have a policy stating that we could only release this to a funeral home. If anyone has a policy and wouldn't mind sharing it with me it would be greatly appreciated. Thanks again, Amy > Amanda (Amy) Garcia > Histology/Pathology > College Station Medical Center > (979) 680-5372 office > (979) 696-5446 fax > *Mailto:amanda.garcia@triadhospitals.com > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the e-mail or any > of its attachments, please be advised that you have received this > e-mail in error and that any use, dissemination, distribution, > forwarding, printing, or copying of this e-mail or any attached files > is strictly prohibited. If you have received this e-mail in error, > please immediately purge it and all attachments and notify the sender > by reply e-mail or contact the sender at the number listed. > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Tue Jul 25 12:49:35 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Tue Jul 25 12:49:49 2006 Subject: [Histonet] Re: Releasing specimens to patients Message-ID: <38c.728c6a9.31f7b3af@aol.com> Amy Garcia asks about releasing fetal remains to patients. I'd have to agree that this is a job for a funeral director. It's certainly something your pathologist should know about and should make the decisions on. There are some real problems with who gets hold of recognizable fetal tissue, which should always be stored and disposed of with great care. A pathologist of my acquaintance - not in a state I've practiced in recently - had an incident where anti-abortion people stole fetuses from his department and harassed the patient whose name appeared on the container (imagine doing that to a woman who's probably mourning the loss of a baby!) I asked the pathologist if he was really sure if that had happened, and he said it definitely had. Bob Richmond From ploykasek <@t> phenopath.com Tue Jul 25 13:14:15 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Tue Jul 25 13:14:22 2006 Subject: [Histonet] Colponin In-Reply-To: <000a01c6b011$15503b30$0711c847@D7XQNX91> Message-ID: Hi Marian. Are you using these antibodies to detect myoepithelium? If so, I would recommend the antibody to smooth muscle myosin heavy chain over calponin. Calponin will also stain the myofibroblasts, and this can make it a bit harder to read in my opinion. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Hi: > > Anyone using colponin? If so, where do I find it? Is it worth having, we > presently use P63 and CD10. > > Thanks in advance, > > Marian > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From LGaliotto <@t> nch.org Tue Jul 25 14:06:25 2006 From: LGaliotto <@t> nch.org (Galiotto, Laura) Date: Tue Jul 25 14:07:44 2006 Subject: [Histonet] Standards for Histology workload Message-ID: <270614B321ACB44D8C1D91F4F921FDC3036CBE8F@NCH01EX02.nch.org> Hello Fellow Histotechs Can anyone direct me to a source which helps estabilsh standards for Histotechs and workload? I have contacted CAP and ASCP without success. I have not been successful contacting CLIA Laura Galiotto, HT (ASCP) Histology Facilitator ******************* PLEASE NOTE ******************* This E-Mail/telefax message and any documents accompanying this transmission may contain information that is privileged, confidential, and/or exempt from disclosure under applicable law and is intended solely for the addressee(s) named above. If you are not the intended addressee/recipient, you are hereby notified that any use of, disclosure, copying, distribution, or reliance on the contents of this E-Mail/telefax information is strictly prohibited and may result in legal action against you. Please reply to the sender advising of the error in transmission and immediately delete/destroy the message and any accompanying documents. Thank you. From LGaliotto <@t> nch.org Tue Jul 25 14:13:00 2006 From: LGaliotto <@t> nch.org (Galiotto, Laura) Date: Tue Jul 25 14:14:21 2006 Subject: [Histonet] Histology workload standards Message-ID: <270614B321ACB44D8C1D91F4F921FDC3036CBE91@NCH01EX02.nch.org> Hello Histotechs Can anyone tell me if they are aware of standards set by any governing body for Histology workload? I have contacted CAP and ASCP without success. I am having difficulties connecting CLIA. Also if you would be so kind and tell me how many techs you have for every 5000 -10000 cases per year. Laura Galiotto, HT (ASCP) Histology Facilitator ******************* PLEASE NOTE ******************* This E-Mail/telefax message and any documents accompanying this transmission may contain information that is privileged, confidential, and/or exempt from disclosure under applicable law and is intended solely for the addressee(s) named above. If you are not the intended addressee/recipient, you are hereby notified that any use of, disclosure, copying, distribution, or reliance on the contents of this E-Mail/telefax information is strictly prohibited and may result in legal action against you. Please reply to the sender advising of the error in transmission and immediately delete/destroy the message and any accompanying documents. Thank you. From Kari.Zajic <@t> HCAhealthcare.com Tue Jul 25 14:25:08 2006 From: Kari.Zajic <@t> HCAhealthcare.com (Zajic Kari) Date: Tue Jul 25 14:25:12 2006 Subject: [Histonet] Re: Histology workload standards Message-ID: <095327C7CDBDF64B9E9728A54799091E015CC573@ORLEV03.hca.corpad.net> Hi Laura... If you find any, please let me know! There seems to be standards set in place for Pathologists and Cytotechnologists but none for Histotechs! Just FYI, we had about 7,500 cases and 600 cytologies with 2 full time techs. It was shear mayhem sometimes! We really needed another tech. Loosing my second tech and never filling the third position, we ended up "outsourcing" to another lab for processing and now with just me, I accession and do all the frozen sections. We are still pulling the same workload numbers, but the processing and cutting/staining,etc is done at another facility. Kari Marie Zajic HT,MLT Histology Supervisor Palms West Hospital Pathology Department 13001 State Road Eighty Loxahatchee, Florida 33470 phone: (561)798-6036 telefax: (561)753-4298 voicemail: (561)753-4299 pager: (561)610-4949 email: Kari.Zajic@HCAHealthcare.com From rjbuesa <@t> yahoo.com Tue Jul 25 14:32:58 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jul 25 14:33:03 2006 Subject: [Histonet] Standards for Histology workload In-Reply-To: <270614B321ACB44D8C1D91F4F921FDC3036CBE8F@NCH01EX02.nch.org> Message-ID: <20060725193258.19444.qmail@web61216.mail.yahoo.com> Laura: I am sending under separate cover a paper I just published on the subject. Hope this will help you. ren? J. "Galiotto, Laura" wrote: Hello Fellow Histotechs Can anyone direct me to a source which helps estabilsh standards for Histotechs and workload? I have contacted CAP and ASCP without success. I have not been successful contacting CLIA Laura Galiotto, HT (ASCP) Histology Facilitator ******************* PLEASE NOTE ******************* This E-Mail/telefax message and any documents accompanying this transmission may contain information that is privileged, confidential, and/or exempt from disclosure under applicable law and is intended solely for the addressee(s) named above. If you are not the intended addressee/recipient, you are hereby notified that any use of, disclosure, copying, distribution, or reliance on the contents of this E-Mail/telefax information is strictly prohibited and may result in legal action against you. Please reply to the sender advising of the error in transmission and immediately delete/destroy the message and any accompanying documents. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail Beta. From rjbuesa <@t> yahoo.com Tue Jul 25 14:34:28 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jul 25 14:34:34 2006 Subject: [Histonet] Histology workload standards In-Reply-To: <270614B321ACB44D8C1D91F4F921FDC3036CBE91@NCH01EX02.nch.org> Message-ID: <20060725193428.72367.qmail@web61223.mail.yahoo.com> Laura: I am attaching under separate cover the draft of an article on Staffing that will nswer these questions for you. Hope this will help you. Ren? J. "Galiotto, Laura" wrote: Hello Histotechs Can anyone tell me if they are aware of standards set by any governing body for Histology workload? I have contacted CAP and ASCP without success. I am having difficulties connecting CLIA. Also if you would be so kind and tell me how many techs you have for every 5000 -10000 cases per year. Laura Galiotto, HT (ASCP) Histology Facilitator ******************* PLEASE NOTE ******************* This E-Mail/telefax message and any documents accompanying this transmission may contain information that is privileged, confidential, and/or exempt from disclosure under applicable law and is intended solely for the addressee(s) named above. If you are not the intended addressee/recipient, you are hereby notified that any use of, disclosure, copying, distribution, or reliance on the contents of this E-Mail/telefax information is strictly prohibited and may result in legal action against you. Please reply to the sender advising of the error in transmission and immediately delete/destroy the message and any accompanying documents. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- See the all-new, redesigned Yahoo.com. Check it out. From tpmorken <@t> labvision.com Tue Jul 25 16:11:24 2006 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Tue Jul 25 16:11:35 2006 Subject: [Histonet] Standards for Histology workload Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA2048F71C9@usca0082k08.labvision.apogent.com> I'll put in a plug for Rene's article ("Removing the Stumbling Blocks; Using Statistics, learn how to optimize your workflow in histology," Advance for Medical Professionals, July 3, 2006, pp. 18 - 29. The major "Inconvenient Truth" that Rene points out is that an increase in the histology workload cannot be handled without either adding more people or sending the work out (which is functionally the same as adding more people). The reason for this is because histology has a very low level of automation. She points out that the clinical lab is mostly automated and one tech can deal with increases in workload by simply feeding more samples into the machines and so greatly increase their personal productivity. The vast majority of work in Histology is still manual so productivity cannot go beyond some relatively finite personal workload levels. Rene suggests that lab managers look at the whole system, not individual tech workload, when trying to increase productivity. Thanks Rene! Tim Morken Lab Vision - Neomarkers www.labvision.com Free webhosting for US State Histotechnology Societies: http://www.labvisioncorp.com/demowebsite/index.cfm -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, July 25, 2006 12:33 PM To: Galiotto, Laura; HISTONET@LISTS.UTSOUTHWESTERN.EDU Subject: Re: [Histonet] Standards for Histology workload Laura: I am sending under separate cover a paper I just published on the subject. Hope this will help you. ren? J. "Galiotto, Laura" wrote: Hello Fellow Histotechs Can anyone direct me to a source which helps estabilsh standards for Histotechs and workload? I have contacted CAP and ASCP without success. I have not been successful contacting CLIA Laura Galiotto, HT (ASCP) Histology Facilitator ******************* PLEASE NOTE ******************* This E-Mail/telefax message and any documents accompanying this transmission may contain information that is privileged, confidential, and/or exempt from disclosure under applicable law and is intended solely for the addressee(s) named above. If you are not the intended addressee/recipient, you are hereby notified that any use of, disclosure, copying, distribution, or reliance on the contents of this E-Mail/telefax information is strictly prohibited and may result in legal action against you. Please reply to the sender advising of the error in transmission and immediately delete/destroy the message and any accompanying documents. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail Beta. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Jul 25 16:44:48 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Jul 25 16:44:52 2006 Subject: [Histonet] Standards for Histology workload In-Reply-To: <0556BE8AC5551E4E8AF6BB9E42509BA2048F71C9@usca0082k08.labvision.apogent.com> Message-ID: <20060725214448.30733.qmail@web61222.mail.yahoo.com> Tim: Just a clarification. I do not say that the work should be outsourced. The fact is that precisely, as you point out, the manual work in histology has a "manual limit". Another clarification: I am a "he", not a "she". Thanks for your comments! Ren? J. "Morken, Tim - Labvision" wrote: I'll put in a plug for Rene's article ("Removing the Stumbling Blocks; Using Statistics, learn how to optimize your workflow in histology," Advance for Medical Professionals, July 3, 2006, pp. 18 - 29. The major "Inconvenient Truth" that Rene points out is that an increase in the histology workload cannot be handled without either adding more people or sending the work out (which is functionally the same as adding more people). The reason for this is because histology has a very low level of automation. She points out that the clinical lab is mostly automated and one tech can deal with increases in workload by simply feeding more samples into the machines and so greatly increase their personal productivity. The vast majority of work in Histology is still manual so productivity cannot go beyond some relatively finite personal workload levels. Rene suggests that lab managers look at the whole system, not individual tech workload, when trying to increase productivity. Thanks Rene! Tim Morken Lab Vision - Neomarkers www.labvision.com Free webhosting for US State Histotechnology Societies: http://www.labvisioncorp.com/demowebsite/index.cfm -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, July 25, 2006 12:33 PM To: Galiotto, Laura; HISTONET@LISTS.UTSOUTHWESTERN.EDU Subject: Re: [Histonet] Standards for Histology workload Laura: I am sending under separate cover a paper I just published on the subject. Hope this will help you. ren? J. "Galiotto, Laura" wrote: Hello Fellow Histotechs Can anyone direct me to a source which helps estabilsh standards for Histotechs and workload? I have contacted CAP and ASCP without success. I have not been successful contacting CLIA Laura Galiotto, HT (ASCP) Histology Facilitator ******************* PLEASE NOTE ******************* This E-Mail/telefax message and any documents accompanying this transmission may contain information that is privileged, confidential, and/or exempt from disclosure under applicable law and is intended solely for the addressee(s) named above. If you are not the intended addressee/recipient, you are hereby notified that any use of, disclosure, copying, distribution, or reliance on the contents of this E-Mail/telefax information is strictly prohibited and may result in legal action against you. Please reply to the sender advising of the error in transmission and immediately delete/destroy the message and any accompanying documents. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail Beta. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- See the all-new, redesigned Yahoo.com. Check it out. From tkngflght <@t> yahoo.com Tue Jul 25 17:01:30 2006 From: tkngflght <@t> yahoo.com (Cheryl) Date: Tue Jul 25 17:03:55 2006 Subject: [Histonet] A word of thanks AND more travel and permanent jobs than we have techs--AGAIN!! In-Reply-To: <20060725193428.72367.qmail@web61223.mail.yahoo.com> Message-ID: <20060725220130.86893.qmail@web50911.mail.yahoo.com> Hi everyone! I want to thank you all for the wonderful responses to my last plea for anyone interested in traveling. We had quite a few responses, a few people are already on the road, others working toward that end and still others asking really great questions and considering the life of a travel tech. (One person got caught in the bad weather near St. Louis and things got a little mixed up---call me if you read this as your cell isn't working either!!) Again--I need temp techs!! There are several openings coming up in good labs where you'll learn a lot, meet some good people and have some fun. Also, we have a stack of permanent openings with upward mobility and great social settings--some in management situations if you're ready to make the leap. I'm the only recruiter who is a working histotech. In the first conversation you get to know me and it's fun. No obligation. No pressure. I don't spam your resume around--it's your life and I'm just here to help out--you make the decisions. Also--we're ramping up to start a Clinical Lab division in a few months...please share our contact information to your MT/MLT friends. Please ask about our referral bonuses. Thanks for sharing your knowledge and time--as always, histotechs are a cut above :) Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab - one tech at a time. 800.756.3309 phone & fax 281.852.9457 office 281.883.7704 cell admin@fullstaff.org www.fullstaff.org From tpmorken <@t> labvision.com Tue Jul 25 17:14:50 2006 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Tue Jul 25 17:15:24 2006 Subject: Clarification...RE: [Histonet] Standards for Histology workload Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA2048F71CD@usca0082k08.labvision.apogent.com> I should clarify that Rene does not specifically mention outsourcing as a solution - that was my logical extention of his (also my mistake!) point. I would also add overtime as essentially a method of "hiring" more technologist time. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim - Labvision Sent: Tuesday, July 25, 2006 2:11 PM To: 'Rene J Buesa'; Galiotto, Laura; HISTONET@LISTS.UTSOUTHWESTERN.EDU Subject: RE: [Histonet] Standards for Histology workload I'll put in a plug for Rene's article ("Removing the Stumbling Blocks; Using Statistics, learn how to optimize your workflow in histology," Advance for Medical Professionals, July 3, 2006, pp. 18 - 29. The major "Inconvenient Truth" that Rene points out is that an increase in the histology workload cannot be handled without either adding more people or sending the work out (which is functionally the same as adding more people). The reason for this is because histology has a very low level of automation. She points out that the clinical lab is mostly automated and one tech can deal with increases in workload by simply feeding more samples into the machines and so greatly increase their personal productivity. The vast majority of work in Histology is still manual so productivity cannot go beyond some relatively finite personal workload levels. Rene suggests that lab managers look at the whole system, not individual tech workload, when trying to increase productivity. Thanks Rene! Tim Morken Lab Vision - Neomarkers www.labvision.com Free webhosting for US State Histotechnology Societies: http://www.labvisioncorp.com/demowebsite/index.cfm -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, July 25, 2006 12:33 PM To: Galiotto, Laura; HISTONET@LISTS.UTSOUTHWESTERN.EDU Subject: Re: [Histonet] Standards for Histology workload Laura: I am sending under separate cover a paper I just published on the subject. Hope this will help you. ren? J. "Galiotto, Laura" wrote: Hello Fellow Histotechs Can anyone direct me to a source which helps estabilsh standards for Histotechs and workload? I have contacted CAP and ASCP without success. I have not been successful contacting CLIA Laura Galiotto, HT (ASCP) Histology Facilitator ******************* PLEASE NOTE ******************* This E-Mail/telefax message and any documents accompanying this transmission may contain information that is privileged, confidential, and/or exempt from disclosure under applicable law and is intended solely for the addressee(s) named above. If you are not the intended addressee/recipient, you are hereby notified that any use of, disclosure, copying, distribution, or reliance on the contents of this E-Mail/telefax information is strictly prohibited and may result in legal action against you. Please reply to the sender advising of the error in transmission and immediately delete/destroy the message and any accompanying documents. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail Beta. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bjdewe <@t> aol.com Tue Jul 25 18:26:57 2006 From: bjdewe <@t> aol.com (bjdewe@aol.com) Date: Tue Jul 25 18:27:07 2006 Subject: [Histonet] IHC animal tissue workshop Message-ID: <8C87E58F4E192E3-534-71E@FWM-D35.sysops.aol.com> We atill have a few spaces for the Animal Tissue Workshop at UC Davis on Aug 25th. Our July 28th Workshop has completely filled. If you are interested in this informative free workshop please go to the link for our flyer and sign up. Space is very limited... http://www.vetmed.ucdavis.edu/vsr/dil/events.html Thank you and see you there! Lorie Live as if you were to die tomorrow. Learn as if you were to live forever. - Gandhi - ******************************************************************* Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s)and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ******************************************************************* ________________________________________________________________________ Check out AOL.com today. Breaking news, video search, pictures, email and IM. All on demand. Always Free. From omnivore98 <@t> yahoo.com Tue Jul 25 18:52:39 2006 From: omnivore98 <@t> yahoo.com (Heather Renko) Date: Tue Jul 25 18:52:43 2006 Subject: [Histonet] PRN Tech in Rockford, Illinois Message-ID: <20060725235239.59974.qmail@web31310.mail.mud.yahoo.com> OSF Saint Anthony in Rockford, Illinois is looking for a PRN histotech. Good pay-completely flexible and will consider any good canidate. Please look online at www.osfhealthcare.org or email me omnivore98@yahoo.com --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail Beta. From Jackie.O'Connor <@t> abbott.com Wed Jul 26 08:58:16 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed Jul 26 08:58:46 2006 Subject: [Histonet] Revisiting Luciferase In-Reply-To: <8C87E58F4E192E3-534-71E@FWM-D35.sysops.aol.com> Message-ID: I've found some old posts about Luciferase in FFPE - - does anyone have a working protocol or suggestions? Thanks, Jackie O' From kmerriam2003 <@t> yahoo.com Wed Jul 26 09:11:22 2006 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Wed Jul 26 09:11:26 2006 Subject: [Histonet] Sakura Rack Adapter for Leica Message-ID: <20060726141122.22141.qmail@web50314.mail.yahoo.com> Funny you should mention that. I just spoke to my Leica rep this morning about this very same thing. He promised to email me the information today. i will forward it when I get it. Kim Merriam "Douglas D. Deltour" wrote: Hello, I am looking for the metal rack adapters that fit on the Sakura slide baskets/rack so I can run them on a Leica stainer. Thanks! Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Music Unlimited - Access over 1 million songs.Try it free. From prouty27 <@t> msn.com Wed Jul 26 09:23:22 2006 From: prouty27 <@t> msn.com (Sally Prouty) Date: Wed Jul 26 09:23:29 2006 Subject: [Histonet] Re: Fluorophore adsorption emission and spectralseparation In-Reply-To: Message-ID: Adam, If your just using 1 antibody and dapi. I would suggest using Alexa555, Texas red or Cy3 depending on your filters. You shouldn't get as much autofluorescence. Also cameras tend to see red better then blues and greens and you get a better signal to noise ratio with red dyes. Sally Sally J. Prouty Research Assistant Howard Hughes Medical Institute/University of Iowa Department of Physiology and Biophysics Kevin Campbell Laboratory 4283 Carver Biomedical Research Building Iowa City, Iowa 52242 phone:319-335-8762 Fax: 319-335-6957 >From: "Melissa Gonzalez" >To: >Subject: [Histonet] Re: Fluorophore adsorption emission and >spectralseparation >Date: Mon, 24 Jul 2006 12:03:36 -0700 > > >Adam, >I've never had much luck visualizing the blue-range emitting dyes. In my >experience, they tend to be very weak and photobleach very easily, which >are sentiments expressed by others I've conferred with as well. AF488 is >usually pretty distinct, so if you are having trouble with this, I would >think AF405 or AF35 would be much worse. DAPI is a very strong nucleic >dye, and unless your filters are shot, should always look really good. >If you have the filters: AF555 (TRITC) or AF594 (Texas red equivalent) >are also very bright. >Also, I am not sure how thick of sections you are using, but brain >tissue structures always pop up a little nicer when you use thicker >sections with fluorescence. Sometimes what may appear wimpy on a thin >section will stand out on a thicker one. > >Good luck, >Melissa > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >histonet-request@lists.utsouthwestern.edu >Sent: Monday, July 24, 2006 10:41 AM >To: histonet@lists.utsouthwestern.edu >Subject: Histonet Digest, Vol 32, Issue 24 > >I had an immunofluorescence question that I'd love to get some input on >from anyone out there. I'm trying to colocalize two antigens in rodent >brain sections. One of the antigens is yielding fairly weak staining- >it also happens to be the one that I'm visualizing with Alexa Fluor 488, >so I'm getting fairly high background autofluorescence- and I think that >the staining could be improved by reducing the autofluorescence. My >DAPI staining (ex/em: 350/470) is great...it only stains nuclei with >minimal autofluorescence. They offer Alexa Fluor 405 streptavidin which >has a similar excit/emission range, so I thought labeling with the Alexa >Fluor 405 would produce a similarly "clean" background as my DAPI >labeling. Also, I already have the filter sets to work with Alexa Fluor >405 (as opposed to going to the other end of the spectrum). > > Has anyone used Alexa Fluor 405? What is your impression of tissue >autofluorescence in that range, photostability/bleaching of the >compounds, comparison of signal using other fluorophores, etc. > > Any input would be greatly appreciated. > Thanks, > Adam Perry > > Department of Neuroscience > University of Illinois at Chicago > Chicago, IL 60612 > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Express yourself instantly with MSN Messenger! Download today - it's FREE! http://messenger.msn.click-url.com/go/onm00200471ave/direct/01/ From oshel1pe <@t> cmich.edu Wed Jul 26 09:34:49 2006 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Wed Jul 26 09:35:20 2006 Subject: [Histonet] Hooker/Lab-line plant microtome Message-ID: Listers, We're trying to find a Parts manual for an old Hooker/Lab-line plant microtome, or an old one of these as a parts donor. For the motor. The company is extinct, bought by Lab-line, and the microtome is Old, enough so that Lab-line has no information on it anymore. Our Hooker fried its motor, and there are NO markings on the motor at all so we can spec a replacement motor. Thanks for any help. Phil -- Philip Oshel Microscopy Facility Supervisor Department of Biology Central Michigan University 024C Brooks Hall Mt. Pleasant, MI 48859 voice: (989) 774-3576 dept. fax: (989) 774-3462 From sbreeden <@t> nmda.nmsu.edu Wed Jul 26 10:57:59 2006 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Jul 26 10:58:06 2006 Subject: [Histonet] Looking for Liz Message-ID: Would Liz Beitman of CellMarque please contact me directly? This relates to the NM Society for Histology. Thanks! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87108 From julie.blake <@t> holburn.com Wed Jul 26 11:08:30 2006 From: julie.blake <@t> holburn.com (Julie Blake) Date: Wed Jul 26 11:08:38 2006 Subject: [Histonet] Job Posting - Histology - Ontario Canada Message-ID: THE HOLBURN GROUP OF COMPANIES, an expanding research and development firm located in the Municipality of Clarington, Durham Region, is focused on innovative research and development programmes in the fields of diagnostics and therapeutics. The projects we undertake both independently and in collaboration with academia and private industry range from early-stage drug discovery to product delivery. We are looking for results-driven professionals to join our team! HISTOLOGY TECHNOLOGIST As a Histology Technologist, reporting to a Team Leader, you will undertake various histological procedures, including tissue processing, tissue sectioning, embedding, staining, sample tracking, and general lab maintenance. Qualifications * Minimum two years practical histochemical experience required. Immunocytochemical and in situ hybridization techniques experience is preferred. * B.Sc. preferred and/or MLT or ART designation. * Highly motivated, flexible and able to thrive in a fast paced environment. * Strong problem-solving, analytical and planning skills, with demonstrated superior written skills Although training will be provided, candidate must be able to work independently upon receipt of instruction. We offer a salary commensurate with skills and experience, a comprehensive benefits package and opportunities for growth and professional development. Please submit a covering letter referencing the position and your curriculum vitae in confidence to hr@holburn.com or by fax to 905-623-6702, attention Human Resources. Thank you in advance for your interest. Only those candidates selected for an interview will be contacted. We are an equal opportunity employer. From julie.blake <@t> holburn.com Wed Jul 26 11:35:24 2006 From: julie.blake <@t> holburn.com (Julie Blake) Date: Wed Jul 26 11:35:36 2006 Subject: [Histonet] Histology Job Posting - Ontario Message-ID: THE HOLBURN GROUP OF COMPANIES, an expanding research and development firm located in the Municipality of Clarington, Durham Region, is focused on innovative research and development programmes in the fields of diagnostics and therapeutics. The projects we undertake both independently and in collaboration with academia and private industry range from early-stage drug discovery to product delivery. We are looking for results-driven professionals to join our team! HISTOLOGY TECHNOLOGIST As a Histology Technologist, reporting to a Team Leader, you will undertake various histological procedures, including tissue processing, tissue sectioning, embedding, staining, sample tracking, and general lab maintenance. Qualifications * Minimum two years practical histochemical experience required. Immunocytochemical and in situ hybridization techniques experience is preferred. * B.Sc. preferred and/or MLT, ART or equivalent designation. * Highly motivated, flexible and able to thrive in a fast paced environment. * Strong problem-solving, analytical and planning skills, with demonstrated superior written skills Although training will be provided, candidate must be able to work independently upon receipt of instruction. We offer a salary commensurate with skills and experience, a comprehensive benefits package and opportunities for growth and professional development. Please submit a covering letter referencing the position and your curriculum vitae in confidence to hr@holburn.com or by fax to 905-623-6702, attention Human Resources. Thank you in advance for your interest. Only those candidates selected for an interview will be contacted. We are an equal opportunity employer. Human Resources Coordinator Holburn Group of Companies Fax: 905 623 6702 E-mail: hr@holburn.com Web: www.holburn.com This transmission may contain information that is privileged, confidential and/or exempt from disclosure under applicable law. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or use of the information contained herein (including any reliance thereon) is STRICTLY PROHIBITED. If you received this transmission in error, please immediately contact the sender and destroy the material in its entirety, whether in electronic or hard copy format. Thank you. From AGrobe2555 <@t> aol.com Wed Jul 26 13:22:49 2006 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Wed Jul 26 13:22:58 2006 Subject: [Histonet] Nitrotyrosine Control Message-ID: <40c.382b8e1.31f90cf9@aol.com> Pedro, I have used sheep lung tissue from 2,4 and 8 week-olds for Nitrotyrosine western blots. Perhaps it might work for IHC? Albert From nyilmaz <@t> mersin.edu.tr Wed Jul 26 21:52:20 2006 From: nyilmaz <@t> mersin.edu.tr (nyilmaz@mersin.edu.tr) Date: Wed Jul 26 13:47:58 2006 Subject: [Histonet] rat chorionic gonadotropin Message-ID: <20060726185220.3CB9B45C7A@mail.mersin.edu.tr> Dear Listers... Does anyone know if a rat chorionic gonadotropin receptor antibody exist suitable for FFPE rat tissues? Thanks in advance... Dr. Necat Yilmaz Mersin University School of Medicine Histology & Embryology Dept. From mhannah <@t> jhsph.edu Wed Jul 26 14:04:38 2006 From: mhannah <@t> jhsph.edu (Hannah, Michele F.) Date: Wed Jul 26 14:03:44 2006 Subject: [Histonet] Texas Red fluorescing when slide viewed through FITC filter References: <5701sv$1dq4si@gateway2.jhsph.edu> Message-ID: <1D71A10BB247204A9EFFB9EED3236058019168B8@XCH-VN02.sph.ad.jhsph.edu> We are currently working on a fluorescent IHC protocol using Vector Lab's Texas Red Avidin DCS and Fluorescein Avidin DCS. Eventually, the slides will be stained with both fluorophores, but for now we are testing with each separately. The problem we are having is slides that are stained with Texas Red will be visible using both the TX Red filter and the FITC filter. We also have the reverse problem, the FITC stained slides show up with both filters. The slides are kept completely separate (different stains are not washed together) throughout the protocol. We are also using DAPI which is included in Vector's Mounting Reagent; that is showing up perfectly. Does anyone have any ideas on what could be causing this? Any help would be appreciated! Michele Michele F. Hannah M.S. Department of Molecular Microbiology & Immunology Johns Hopkins Bloomberg School of Public Health 615 North Wolfe Street Room E3201 Baltimore, Maryland 21205 Phone: (410) 614-7794 Fax: (410) 955-0105 From Rcartun <@t> harthosp.org Wed Jul 26 14:16:55 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Jul 26 14:17:30 2006 Subject: [Histonet] Upcoming IHC meeting Message-ID: <44C78767020000770000115F@hcnwgwds01.hh.chs> Dear Colleagues: The Society for Applied Immunohistochemistry has been inactive for some time now. I am happy to report that "The First International Course on Applied Immunohistochemistry and Molecular Morphology" will be held on January 23-26, 2007 in Duck Key, Florida, U.S.A. This exciting meeting has been organized by Dr. Hadi Yaziji, President of Ancillary Pathways in Miami, FL and is being sponsored by the Society for Applied Immunohistochemistry. You can obtain additional information and register by going to www.pathlearning.com. Please contact me if you have any questions. Also, please provide me with your thoughts and suggestions regarding the future of our Society. Please let me know how we can help those of you working in the field of immunohistochemistry. Best regards, Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From TerriGillow <@t> warrenhospital.org Wed Jul 26 14:18:40 2006 From: TerriGillow <@t> warrenhospital.org (Terri Gillow) Date: Wed Jul 26 14:18:44 2006 Subject: [Histonet] (no subject) Message-ID: <2FDC4BEE974F1E44A525F9E3DB9A9EC2A84A3C@mercury.warrenhospital.org> I am curious if anyone has ever heard of a "mucin blue day". Once in a great while our H&E's on our GI biopsies have a light blue hue in the area that should be clear. There is no rhyme or reason to it. Any suggestions on how to figure out why it is happening and how I can prevent it? *** CONFIDENTIALITY NOTICE *** The information in this email, including attachments, may be confidential and/or privileged and may contain confidential health information. This email is intended to be reviewed only by the individual or organization named as addressee. If you have received this email in error please notify the sender immediately and destroy all copies of this message and any attachments. Please note that any views or opinions presented in this email are solely those of the author and do not necessarily represent those of Warren Hospital. Confidential health information is protected by state and federal law, including, but not limited to, the Health Insurance Portability and Accountability Act of 1996 and related regulations From asmith <@t> mail.barry.edu Wed Jul 26 14:29:05 2006 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Wed Jul 26 14:29:10 2006 Subject: [Histonet] (no subject) In-Reply-To: <2FDC4BEE974F1E44A525F9E3DB9A9EC2A84A3C@mercury.warrenhospital.org> Message-ID: <7DBCCC1FBC77C94F99F920D0CA6400B61E3FAF@exchsrv01.barrynet.barry.edu> This occasionally happens to me. It seems to be caused by the pH of the hematoxylin solution or of the first wash water being too high. To be specific for nuclei, hematoxylin should be fairly acid. Most formulas rely on the alum for acidity. I prefer Ehrlich's formula which is 3% acetic acid. Ehrlich's hematoxylin does become less acid over time, due to the reaction of the acetic acid with the ethanol. (Old Ehrlich hematoxylin reeks of ethyl acetate.) Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Gillow Sent: Wednesday, July 26, 2006 3:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) I am curious if anyone has ever heard of a "mucin blue day". Once in a great while our H&E's on our GI biopsies have a light blue hue in the area that should be clear. There is no rhyme or reason to it. Any suggestions on how to figure out why it is happening and how I can prevent it? *** CONFIDENTIALITY NOTICE *** The information in this email, including attachments, may be confidential and/or privileged and may contain confidential health information. This email is intended to be reviewed only by the individual or organization named as addressee. If you have received this email in error please notify the sender immediately and destroy all copies of this message and any attachments. Please note that any views or opinions presented in this email are solely those of the author and do not necessarily represent those of Warren Hospital. Confidential health information is protected by state and federal law, including, but not limited to, the Health Insurance Portability and Accountability Act of 1996 and related regulations _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From pruegg <@t> ihctech.net Wed Jul 26 14:36:10 2006 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Jul 26 14:36:00 2006 Subject: [Histonet] paraffin ribboning Message-ID: <200607261935.k6QJZqqX026234@chip.viawest.net> I am posting this for Johnathan, please respond directly to him, he is not on histonet jjones@gadermpath.com "Patsy I have a small trouble shooting problem non ihc related I would like to ask you. For the last 4 weeks we have had problems with ribbioning. We have change angles, temp in lab, also the embedding paraffin. Can you advise me on how to correct the issue. Any help you could provide I would greatly appreciate." Jonathan Jones Gadermpath Atlanta, ga Ht Ascp/ supervisor Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From AGrobe2555 <@t> aol.com Wed Jul 26 17:07:13 2006 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Wed Jul 26 17:07:20 2006 Subject: [Histonet] Texas Red fluorescing when slide viewed through FITC filter Message-ID: <548.43a5f3c.31f94191@aol.com> Have you checked the bandpass of the filter? It sound like you have some overlap in the emission that shows up in either filter. From lpwenk <@t> sbcglobal.net Thu Jul 27 03:27:52 2006 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Thu Jul 27 03:28:44 2006 Subject: [Histonet] Implications of Various Fixatives In-Reply-To: <5a6e501c6b012$14e43d80$6b05010a@mfldclinframe.org> Message-ID: <000601c6b156$8da8c6b0$58a04d44@HPPav2> The September 2001 (Vol. 24, No. 3) issue of the Journal of Histotechnology had a lot of good articles on fixation. M. Lamar Jones. To Fix, To Harden, To Preserve - Fixation: A Brief History. pp. 155-162 Michael Titford. Safety Considerations in the Use of Fixatives. pp. 165-171 Isam Eltoum, Jerry Fredenburgh, Russel B. Myers, William E. Grizzle. Introduction to the Theory and Practice of Fixation of Tissue. pp. 181-190 (with great charts. One has the different types of fixatives and how they effect proteins / lipids / RNA / DNA / Ultrastructure as well as time issues and special uses. A second chart lists required/best fixative for histology special stains and/or fixatives to be avoided. It also has formulas for a lot of different fixatives.) Isam Eltoum, Jerry Fredenburgh, William E. Grizzle. Advanced Concepts in Fixation: 1. Effects of Fixation on Immunohistochemistry, Reversibility of Fixation and Recovery of Proteins, Nucleic Acids, and other Molecules from Fixed and Processed Tissues. 2. Developmental Methods of Fixation. pp. 201-210. William E. Grizzle, Cecil R. Stockard, Paul E. Billings. The Effects of Tissue Processing Variables Other than Fixation on Histochemical Staining and Immunohistochemical Detection of Antigens. pp. 213 - 219. (I know this has nothing to do with fixation, but processing DOES influence staining, so I thought I'd add it.) These don't quite cover everything you were asking, but it's a start. A lot of "don't use fixative X when needing to demonstrate Ab Y" is found on each manufacturer's specification sheet for the antibodies. Possibly the NSH Office still has copies of this issue (for a price). I don't know. 301-262-6221 or histo@nsh.org Or possibly the authors of these articles are histonetters, and might still have author copies, and would let you know. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of bliven.laura@marshfieldclinic.org Sent: Tuesday, July 25, 2006 1:45 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Implications of Various Fixatives Does anyone know of a good published reference addressing various fixatives and their reactions on certain immuno's, special stains and artifacts? I understand that there is a chance that tissue fixed in Bouin and Hollande fixatives can cause false-negative immuno results in some lymphoma markers and found another mention of a certain fixative causing a false-negative result in the Steiner and Steiner. Also it would be great to see the pH of all the fixatives along with information such as "Do not allow Hollande's fixed specimens to come in contact with phosphate buffered formalin, a blue precipitate (artifact) occurs" - and is this what some articles refer to as being "incompatible with NBF"? Thanks much, Laura Laura Bliven Histology Lab/IHC Marshfield Laboratories 1000 N. Oak Ave. Marshfield, WI 54449 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From STapper <@t> slhduluth.com Thu Jul 27 07:10:31 2006 From: STapper <@t> slhduluth.com (Tapper, Sheila) Date: Thu Jul 27 07:10:37 2006 Subject: [Histonet] A mystery... Message-ID: We are having a random problem that I hope someone can help me solve. We routinely cut extra sections on our prostate needle biopsies for immuno stains - specifically the PIN 4 antibody. In a very random manner, we will have an occasional case that will not stay adhered to the slide. Some days we can have 4 cases, cut by the same tech, handled together, and one case will wash off. If we repeat using different levels - they wash again. We retrieve the slides in a high pH retrieval solution prior to staining. We do not see the same effect on H&E - they routinely stay on. We are using coated slides for everything - using the same batch, and seeing the same phenomenon. I can only wonder if there is something happening to the biopsy at the time of collection that may inhibit the tissue from staying adhered to the slide???? (I am always hopeful the reason is outside the lab!!!!) Thanks for any insights! Sheila Tapper HT(ASCP) St. Luke's Hospital Duluth, MN From JMansfield <@t> cri-inc.com Thu Jul 27 09:03:49 2006 From: JMansfield <@t> cri-inc.com (Mansfield, James) Date: Thu Jul 27 09:06:48 2006 Subject: [Histonet] Texas Red fluorescing when slide viewed through FITCfilter Message-ID: Hi Hannah, Depending on the brightness of the fluorophores you are using, it is quite possible for the FITC to show up in the Texas Red channel. There is a significant (about 10% of max) FITC emission at 600 nm (the peak of Texas Red emission). You can plot out the spectra of various fluorophores at: http://probes.invitrogen.com/resources/spectraviewer/ Unfortunately, there are only ways to prevent the FITC from bleeding through: 1. Reduce the amount of FITC on your slide so that the 10% bleed through is not visually apparent. 2. Use a multispectral imaging approach and linear unmixing to separate the contributions of the two fluorophores from each other. Regards, -Jim -- James R. Mansfield Product Manager, MSI Systems Senior Spectral Imaging Scientist CRi 35B Cabot Road Woburn, MA, 01801 Email: jmansfield@cri-inc.com Web: www.cri-inc.com Phone: +1-781-935-9099 x 117 > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf > Of Hannah, Michele F. > Sent: Wednesday, July 26, 2006 3:05 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Texas Red fluorescing when slide viewed > through FITCfilter > > We are currently working on a fluorescent IHC protocol using > Vector Lab's Texas Red Avidin DCS and Fluorescein Avidin DCS. > Eventually, the slides will be stained with both > fluorophores, but for now we are testing with each > separately. The problem we are having is slides that are > stained with Texas Red will be visible using both the TX Red > filter and the FITC filter. We also have the reverse > problem, the FITC stained slides show up with both filters. > The slides are kept completely separate (different stains are > not washed together) throughout the protocol. We are also > using DAPI which is included in Vector's Mounting Reagent; > that is showing up perfectly. > > Does anyone have any ideas on what could be causing this? > Any help would be appreciated! > > Michele > > Michele F. Hannah M.S. > Department of Molecular Microbiology & Immunology Johns > Hopkins Bloomberg School of Public Health > 615 North Wolfe Street Room E3201 > Baltimore, Maryland 21205 > Phone: (410) 614-7794 > Fax: (410) 955-0105 > > > From TerriGillow <@t> warrenhospital.org Thu Jul 27 09:24:27 2006 From: TerriGillow <@t> warrenhospital.org (Terri Gillow) Date: Thu Jul 27 09:24:31 2006 Subject: [Histonet] Paraffin-resistant flooring Message-ID: <2FDC4BEE974F1E44A525F9E3DB9A9EC2A84ADC@mercury.warrenhospital.org> Have any of you had the experience of working in a lab with such a floor? If so, would you recommend it? Is it worth the cost? Thanks *** CONFIDENTIALITY NOTICE *** The information in this email, including attachments, may be confidential and/or privileged and may contain confidential health information. This email is intended to be reviewed only by the individual or organization named as addressee. If you have received this email in error please notify the sender immediately and destroy all copies of this message and any attachments. Please note that any views or opinions presented in this email are solely those of the author and do not necessarily represent those of Warren Hospital. Confidential health information is protected by state and federal law, including, but not limited to, the Health Insurance Portability and Accountability Act of 1996 and related regulations From histo20 <@t> hotmail.com Thu Jul 27 09:48:57 2006 From: histo20 <@t> hotmail.com (Paula Wilder) Date: Thu Jul 27 09:49:07 2006 Subject: [Histonet] Job posting in the Towson (Baltimore), MD area Message-ID: Hi everyone, St. Joseph Medical Center has 2 openings for full-time registered Histotechnicians. 40-hour work week; salary - negotiable. Hospital is progressive and growing! If interested for details please contact HR at 410-337-1288. Paula Wilder, Histology Supervisor St. Joseph Medical Center 7601 Osler Drive Towson, MD 21204 410-337-1741 From juan.gutierrez <@t> christushealth.org Thu Jul 27 10:06:42 2006 From: juan.gutierrez <@t> christushealth.org (GUTIERREZ, JUAN) Date: Thu Jul 27 10:06:49 2006 Subject: [Histonet] Paraffin-resistant flooring Message-ID: My previous place of employment had a vinyl tile floor with a rough surface on it. I can't remember where we got it from, but it worked really well. When the docs or sales reps came in with their fancy shoes, they did not have the ice skating problems you get with regular floors. I believe these tiles only cost a little more than regular vinyl tiles which don't cost much to begin with. Hope this helps. Juan C. Gutierrez, HT(ASCP) Histology Laboratory Supervisor (210)704-2533 My opinions are my own and do not reflect those of my employer. Long live free speech! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Gillow Sent: Thursday, July 27, 2006 9:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Paraffin-resistant flooring Have any of you had the experience of working in a lab with such a floor? If so, would you recommend it? Is it worth the cost? Thanks *** CONFIDENTIALITY NOTICE *** The information in this email, including attachments, may be confidential and/or privileged and may contain confidential health information. This email is intended to be reviewed only by the individual or organization named as addressee. If you have received this email in error please notify the sender immediately and destroy all copies of this message and any attachments. Please note that any views or opinions presented in this email are solely those of the author and do not necessarily represent those of Warren Hospital. Confidential health information is protected by state and federal law, including, but not limited to, the Health Insurance Portability and Accountability Act of 1996 and related regulations _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dpahisto <@t> yahoo.com Thu Jul 27 10:33:22 2006 From: dpahisto <@t> yahoo.com (Cindy DuBois) Date: Thu Jul 27 10:33:29 2006 Subject: [Histonet] Tissue-Tek Parts Message-ID: <20060727153322.11736.qmail@web33402.mail.mud.yahoo.com> If anyone has a "non-working" tissue-tek embedding center model# 4585, specifically the thermo unit, we are looking for a part. In the midst of remodeling and moving we lost the fuse and fuse holder that is on the back side of the machine. We have contacted several places including sakura and our local repair businesses (as well as radio shack), and no one has this part. We really need to get this embedding center back up and running ASAP. If you have the part or know of someone who might, please let me know, thanks, Cindy DuBois Integrated Pathology Services Stockton, CA --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. From Jessica.Vacca <@t> HCAhealthcare.com Thu Jul 27 10:42:44 2006 From: Jessica.Vacca <@t> HCAhealthcare.com (Vacca Jessica) Date: Thu Jul 27 10:42:51 2006 Subject: [Histonet] CAP questions regarding Microwaves Message-ID: <41E16A15CE78374EA45B57E0F94339B801675493@ORLEV01.hca.corpad.net> Hi all, I went through the archive to find out information regarding the new Cap questions on microwaves and there were no definite answers.....Can I get a some feedback on the proposed methods for being compliant with these questions. Here are a couple of ideas that I had. Annual testing- I purchased a leakage detector (35.00 on line) and will do an annual test (or is this something Biomed should be doing?) monitoring temps- 3 coplin jars temps prior to going in taking start temps and 1 at a time place the containers in the exact same spot, 50 mls tap water for 30 secs. Take temp ea time. It gives me a reading of 63 degrees, there can be a little flexibility but by how much does one recommend? Also what is everyone considering periodically? 1 a month? As far as the venting goes, we use a wal-mart purchased microwave, I read in an archive that these are not allowed according to OSHA. Has everyone out there thrown away there microwaves that you've been using all these years, or are you continuing to use them? Thanks in advance for your feedback! Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr. Brandon Fl 33511 (813) 571-5193 or (813) 681-5551 ext 2454 Jessica.Vacca@hcahealthcare.com From doug <@t> ppspath.com Thu Jul 27 11:12:27 2006 From: doug <@t> ppspath.com (Douglas D. Deltour) Date: Thu Jul 27 11:09:29 2006 Subject: [Histonet] South Carolina Society for Histotechnology Message-ID: I am looking for the contact info of the head of the SCSH. Thanks Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. From la.sebree <@t> hosp.wisc.edu Thu Jul 27 13:07:31 2006 From: la.sebree <@t> hosp.wisc.edu (Sebree Linda A.) Date: Thu Jul 27 13:07:37 2006 Subject: [Histonet] Matriptase (aka ST14, SNC19, SNC-19, Epitin, Prostamin, TADG-15, MT-SP1, MTSP1 Message-ID: Hello everyone, Wondering if anyone has any experience staining for this antigen in FFPE sections. Your help, as always, is greatly appreciated. Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 From kjohnson <@t> covx.com Thu Jul 27 13:31:32 2006 From: kjohnson <@t> covx.com (Kimberly Johnson) Date: Thu Jul 27 13:31:36 2006 Subject: [Histonet] cFos staining in mouse brain Message-ID: <96DACB13ECACA645A47020B3E136776C0FA20C@covxmail.covx.com> Hi, I am looking to do a DAB stain for cFos in the mouse brain. Was wondering if anyone had any protocols they could share? I am not sure whether to do it formalin fixed or frozen, it doesn't matter to us as long as we get a clear stain. Thanks so much for your time and help. Kim From ryaskovich <@t> dir.nidcr.nih.gov Thu Jul 27 13:37:47 2006 From: ryaskovich <@t> dir.nidcr.nih.gov (Ruth Yaskovich) Date: Thu Jul 27 13:38:02 2006 Subject: [Histonet] cFos staining in mouse brain In-Reply-To: <96DACB13ECACA645A47020B3E136776C0FA20C@covxmail.covx.com> Message-ID: Kimberly, I use Vector's Dab on my rat brains for cFos and get beautiful results. Rats are perfused in 4% Para. Good luck Ruth Yaskovich National Institutes of Health National Institute of Dental and Craniofacial Research Pain and Neurosensory Mechanism Branch On 7/27/06 2:31 PM, "Kimberly Johnson" wrote: > Hi, > > I am looking to do a DAB stain for cFos in the mouse brain. Was > wondering if anyone had any protocols they could share? I am not sure > whether to do it formalin fixed or frozen, it doesn't matter to us as > long as we get a clear stain. > > > > Thanks so much for your time and help. > > > > Kim > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dmikita <@t> wmcnet.org Thu Jul 27 13:42:36 2006 From: dmikita <@t> wmcnet.org (Daryl Mikita) Date: Thu Jul 27 13:43:03 2006 Subject: [Histonet] Manual Microtome? Message-ID: Hello, Seeing I haven't been to a National meeting for awhile. I was wondering what companies are still out there that make a quality manual microtome? I know of Sakura, Leica and Olympus, but only have contacts for Sakura and Leica. If there are any sales people looking at this for the Wyoming area, please send information to me at the address at the bottom of this message. Also, for the tech's reading this. What in your opinion is the best (all areas) manual microtome out there? I currently have a Microm Ergostar and hate it. I have spent thousands of dollars trying to get it to not do the venetian blinding on 95% of our blocks, but it still does it. It doesn't make any difference as to what type os tissue I cut or the size. Thanks in advance, Daryl A. Mikita, HT(ASCP)cm Wyoming Medical Center Anatomical Pathology 1233 E. 2nd St. Casper, WY 82601 From Kari.Zajic <@t> HCAhealthcare.com Thu Jul 27 13:49:24 2006 From: Kari.Zajic <@t> HCAhealthcare.com (Zajic Kari) Date: Thu Jul 27 13:49:28 2006 Subject: [Histonet] Manual Microtome? In-Reply-To: Message-ID: <095327C7CDBDF64B9E9728A54799091E015CC583@ORLEV03.hca.corpad.net> I happen to ADORE my Leica RM 2125..I have never had any problems, aside from the occasional blade grip reinforement being replaced after thousands of blocks have been cut, and it being pretty expensive to replace them. I have never used anything but a manual microtome and I am partial to Leica products... Kari Marie Zajic HT,MLT Histology Supervisor Palms West Hospital Pathology Department 13001 State Road Eighty Loxahatchee, Florida 33470 phone: (561)798-6036 telefax: (561)753-4298 voicemail: (561)753-4299 pager: (561)610-4949 email: Kari.Zajic@HCAHealthcare.com This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Daryl Mikita Sent: Thursday, July 27, 2006 2:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Manual Microtome? Hello, Seeing I haven't been to a National meeting for awhile. I was wondering what companies are still out there that make a quality manual microtome? I know of Sakura, Leica and Olympus, but only have contacts for Sakura and Leica. If there are any sales people looking at this for the Wyoming area, please send information to me at the address at the bottom of this message. Also, for the tech's reading this. What in your opinion is the best (all areas) manual microtome out there? I currently have a Microm Ergostar and hate it. I have spent thousands of dollars trying to get it to not do the venetian blinding on 95% of our blocks, but it still does it. It doesn't make any difference as to what type os tissue I cut or the size. Thanks in advance, Daryl A. Mikita, HT(ASCP)cm Wyoming Medical Center Anatomical Pathology 1233 E. 2nd St. Casper, WY 82601 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Janet.Bonner <@t> FLHOSP.ORG Thu Jul 27 14:16:26 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Thu Jul 27 14:18:06 2006 Subject: [Histonet] Manual Microtome? References: <095327C7CDBDF64B9E9728A54799091E015CC583@ORLEV03.hca.corpad.net> Message-ID: Ditto, Ditto, Ditto - we have five of them and they have been with us for at least the eight years I've been here!! Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Zajic Kari Sent: Thu 7/27/2006 2:49 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Manual Microtome? I happen to ADORE my Leica RM 2125..I have never had any problems, aside from the occasional blade grip reinforement being replaced after thousands of blocks have been cut, and it being pretty expensive to replace them. I have never used anything but a manual microtome and I am partial to Leica products... Kari Marie Zajic HT,MLT Histology Supervisor Palms West Hospital Pathology Department 13001 State Road Eighty Loxahatchee, Florida 33470 phone: (561)798-6036 telefax: (561)753-4298 voicemail: (561)753-4299 pager: (561)610-4949 email: Kari.Zajic@HCAHealthcare.com This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Daryl Mikita Sent: Thursday, July 27, 2006 2:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Manual Microtome? Hello, Seeing I haven't been to a National meeting for awhile. I was wondering what companies are still out there that make a quality manual microtome? I know of Sakura, Leica and Olympus, but only have contacts for Sakura and Leica. If there are any sales people looking at this for the Wyoming area, please send information to me at the address at the bottom of this message. Also, for the tech's reading this. What in your opinion is the best (all areas) manual microtome out there? I currently have a Microm Ergostar and hate it. I have spent thousands of dollars trying to get it to not do the venetian blinding on 95% of our blocks, but it still does it. It doesn't make any difference as to what type os tissue I cut or the size. Thanks in advance, Daryl A. Mikita, HT(ASCP)cm Wyoming Medical Center Anatomical Pathology 1233 E. 2nd St. Casper, WY 82601 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Xilong.Li <@t> UTSouthwestern.edu Thu Jul 27 14:38:45 2006 From: Xilong.Li <@t> UTSouthwestern.edu (Xilong Li) Date: Thu Jul 27 14:38:53 2006 Subject: [Histonet] How to stain frozen section with Gomori trichrome? Message-ID: <44C8CFF5020000F000004ABD@swnw124.swmed.edu> Hi, All members, I am a new member for histonet. I'm looking for a consistent protocol for a Gomori trichrome stain for frozen section. I suppose this topic was discussed previously, but I can not get exact answer when I try to review early messages. So I send messages here. I tried to stain frozen muscle section with gomori trichrome but failed recently. My protocol is as follow in brief: 1 frozen section was brought to room temperature before staining which was token from -80C. 2 stain nuclei with Weigert's Iron haematoxylin (sigma) for 15min 3 wash in distilled water for 1min 4 stain in staining solution (Chromotrope 2R 0.6g, fast green FCF 0.3g, Phosphotungstic acid 0.6g, Glacial acetic acid 1.0ml, distilled water 100ml, adjust pH to 3.4) for 12min 5 rinse in 1%(50ul/50ml) acetic acid in dips 6 dehydration with 100% alcohols in dips 7 put section in xylene 1min 8 mount The pictures was so bad without the red color for cytoplasma, almost all part of muscle tissue or cytoplasma was green or dark(which suppose to be red color), it was so hard to make clear where is collagen, or nuclei which was stained in funny red color at some extent. I changed formula of one step stain solution (Chromotrope 2R 0.6g, fast green FCF 0.1g, Phosphotungstic acid 0.8g, Glacial acetic acid 1.0ml, distilled water 100ml, adjust pH to 3.4) according to sigma formula of commercially prepared solution, and stained again, no improvement was gotten. So I am just looking forward to hearing from any guys, who can give me some suggestions what was wrong with my current protocol, and how to improve it, or just email me the protocol which works in their labs. My email is: xilong.li@utsouthwestern.edu, or just fax me 214-648-7902. Thanks in advance. Dr. Xilong Li Hypertension Division, Internal Medicine University of Texas Southwestern Medical Center 5323 Harry Hiness Blvd-J4.142 Dallas, TX 75390 Tel: 214-648-9966(L) Fax: 214-648-7902 From rjbuesa <@t> yahoo.com Thu Jul 27 15:16:06 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Jul 27 15:16:10 2006 Subject: [Histonet] CAP questions regarding Microwaves In-Reply-To: <41E16A15CE78374EA45B57E0F94339B801675493@ORLEV01.hca.corpad.net> Message-ID: <20060727201606.1626.qmail@web61217.mail.yahoo.com> Jessica: 1- About the "household" MW oven: I do not think that everybody has gottent rid of them, but they are not allowed to be used in the lab. I you are using either formalin (tissue fixation) or flammables (like ethanol during staining) the lab MWoven is required to be connected with an exhaust and the household MWoven do not have that feature. You can get in "trouble" during an inspection. 2- MWoven leakage: I will tell you what I used to do. Our MWovens were tested for leakage twice a year by the lab safety officer (or designee). The results were logged. By the way I had two lab MWovens (1 for rapid tissue processing and another for HC staining) both vented. I also had one household MWoven used only to heat the HIER buffers. Never had a problem with CAP. 3- About temperatures in the solutions. You should calibrate your MWoven and prepare heating curves for different liquid volumes and times. I am sending under separate cover a paper I published about calibrating MW ovens. Hope this will help you. Ren? J. Vacca Jessica wrote: Hi all, I went through the archive to find out information regarding the new Cap questions on microwaves and there were no definite answers.....Can I get a some feedback on the proposed methods for being compliant with these questions. Here are a couple of ideas that I had. Annual testing- I purchased a leakage detector (35.00 on line) and will do an annual test (or is this something Biomed should be doing?) monitoring temps- 3 coplin jars temps prior to going in taking start temps and 1 at a time place the containers in the exact same spot, 50 mls tap water for 30 secs. Take temp ea time. It gives me a reading of 63 degrees, there can be a little flexibility but by how much does one recommend? Also what is everyone considering periodically? 1 a month? As far as the venting goes, we use a wal-mart purchased microwave, I read in an archive that these are not allowed according to OSHA. Has everyone out there thrown away there microwaves that you've been using all these years, or are you continuing to use them? Thanks in advance for your feedback! Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr. Brandon Fl 33511 (813) 571-5193 or (813) 681-5551 ext 2454 Jessica.Vacca@hcahealthcare.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Music Unlimited - Access over 1 million songs.Try it free. From Charles.Embrey <@t> carle.com Thu Jul 27 15:28:39 2006 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Thu Jul 27 15:28:45 2006 Subject: [Histonet] off topic news from C. Embrey Message-ID: This is very "off topic" but I had to share this news with my friends on histonet. My first novel, The Lost Keep of Kaywall (ISBN1-58982-369-9), is being released from the publisher and will make its debut at Gen Con in Indianapolis on Aug 10th. It is available online at my website www.GreyRealm.com and at Publisher's Direct Bookstore at www.pdbookstore.com . It will also be coming soon to amazon.com. It is a fantasy adventure in the Dungeons and Dragons setting and is basically a moralistic good verses evil adventure. It was something I started for fun and it has actually taken form. More information is available on my website. I apologize in advance to anyone troubled that this is not "histology" related but I have been with histonet for so many years now that I think of you as my extended family. Thanks, Charles Embrey Jr. From JWEEMS <@t> sjha.org Thu Jul 27 15:30:33 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Thu Jul 27 15:30:51 2006 Subject: [Histonet] off topic news from C. Embrey Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202960512@sjhaexc02.sjha.org> And we are! Thanks for sharing and congrats... wow, we can say we knew you when! Best wishes for more... j Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From weneng2004 <@t> yahoo.com Thu Jul 27 17:10:32 2006 From: weneng2004 <@t> yahoo.com (wen eng) Date: Thu Jul 27 17:10:36 2006 Subject: [Histonet] image software Message-ID: <20060727221032.67360.qmail@web53412.mail.yahoo.com> Hello, Is anybody using Nikon NIS-Elements software? What's good and not-so good about it? I am looking for a software to capture my staining image and do basic analysis. Thanks, Wen --------------------------------- Do you Yahoo!? Next-gen email? Have it all with the all-new Yahoo! Mail Beta. From tonia.richmond <@t> thermo.com Thu Jul 27 19:47:10 2006 From: tonia.richmond <@t> thermo.com (Richmond, Tonia) Date: Thu Jul 27 19:47:16 2006 Subject: [Histonet] CAP questions regarding Microwaves Message-ID: It was my understanding that the store purchased units must be vented, meaning under a hood if the unit does not offer an adapter for venting. Best Regards, Tonia J. Richmond, HT (ASCP) Sales Representative Clinical Diagnostics - Anatomical Pathology Thermo Electron Corporation 171 Industry Drive Pittsburgh, PA 15275-1034 Phone: (800) 245-6212, ext. 4739 Cell: (870) 575-3307 Fax: (870) 879-9639 Email: tonia.richmond@thermo.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vacca Jessica Sent: Thursday, July 27, 2006 10:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] CAP questions regarding Microwaves Hi all, I went through the archive to find out information regarding the new Cap questions on microwaves and there were no definite answers.....Can I get a some feedback on the proposed methods for being compliant with these questions. Here are a couple of ideas that I had. Annual testing- I purchased a leakage detector (35.00 on line) and will do an annual test (or is this something Biomed should be doing?) monitoring temps- 3 coplin jars temps prior to going in taking start temps and 1 at a time place the containers in the exact same spot, 50 mls tap water for 30 secs. Take temp ea time. It gives me a reading of 63 degrees, there can be a little flexibility but by how much does one recommend? Also what is everyone considering periodically? 1 a month? As far as the venting goes, we use a wal-mart purchased microwave, I read in an archive that these are not allowed according to OSHA. Has everyone out there thrown away there microwaves that you've been using all these years, or are you continuing to use them? Thanks in advance for your feedback! Jessica Vacca Histology Supervisor Brandon Regional Hospital 119 Oakfield Dr. Brandon Fl 33511 (813) 571-5193 or (813) 681-5551 ext 2454 Jessica.Vacca@hcahealthcare.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tonia.richmond <@t> thermo.com Thu Jul 27 19:51:25 2006 From: tonia.richmond <@t> thermo.com (Richmond, Tonia) Date: Thu Jul 27 19:51:32 2006 Subject: [Histonet] Manual Microtome? Message-ID: Thermo offers an excellent manual microtome at an affordable price. You can contact your local sales rep by calling 800-245-6212 and ask for Customer Service. Best Regards, Tonia J. Richmond, HT (ASCP) Sales Representative Clinical Diagnostics - Anatomical Pathology Thermo Electron Corporation 171 Industry Drive Pittsburgh, PA 15275-1034 Phone: (800) 245-6212, ext. 4739 Cell: (870) 575-3307 Fax: (870) 879-9639 Email: tonia.richmond@thermo.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Daryl Mikita Sent: Thursday, July 27, 2006 1:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Manual Microtome? Hello, Seeing I haven't been to a National meeting for awhile. I was wondering what companies are still out there that make a quality manual microtome? I know of Sakura, Leica and Olympus, but only have contacts for Sakura and Leica. If there are any sales people looking at this for the Wyoming area, please send information to me at the address at the bottom of this message. Also, for the tech's reading this. What in your opinion is the best (all areas) manual microtome out there? I currently have a Microm Ergostar and hate it. I have spent thousands of dollars trying to get it to not do the venetian blinding on 95% of our blocks, but it still does it. It doesn't make any difference as to what type os tissue I cut or the size. Thanks in advance, Daryl A. Mikita, HT(ASCP)cm Wyoming Medical Center Anatomical Pathology 1233 E. 2nd St. Casper, WY 82601 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech <@t> charter.net Thu Jul 27 20:07:45 2006 From: histotech <@t> charter.net (histotech@charter.net) Date: Thu Jul 27 20:07:51 2006 Subject: [Histonet] Manual Microtome? Message-ID: <909217342.1154048865191.JavaMail.root@fepweb03> ---- Daryl Mikita wrote: > Hello, > > Seeing I haven't been to a National meeting for awhile. I was wondering what companies are still out there that make a quality manual microtome? I know of Sakura, Leica and Olympus, but only have contacts for Sakura and Leica. If there are any sales people looking at this for the Wyoming area, please send information to me at the address at the bottom of this message. > > Also, for the tech's reading this. What in your opinion is the best (all areas) manual microtome out there? I currently have a Microm Ergostar and hate it. I have spent thousands of dollars trying to get it to not do the venetian blinding on 95% of our blocks, but it still does it. It doesn't make any difference as to what type os tissue I cut or the size. > > > Thanks in advance, > > > Daryl A. Mikita, HT(ASCP)cm > Wyoming Medical Center > Anatomical Pathology > 1233 E. 2nd St. > Casper, WY 82601 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet I'll have to vote for Leica !! From gu.lang <@t> gmx.at Fri Jul 28 01:59:41 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Jul 28 01:59:43 2006 Subject: AW: [Histonet] Manual Microtome? In-Reply-To: Message-ID: <001401c6b213$666b2b20$eeeea8c0@SERVER01> You speak about rotation microtome,don't you? I've been working with a microm sliding microtom for years - and love it. Tastes are different. Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Daryl Mikita Gesendet: Donnerstag, 27. Juli 2006 20:43 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Manual Microtome? Hello, Seeing I haven't been to a National meeting for awhile. I was wondering what companies are still out there that make a quality manual microtome? I know of Sakura, Leica and Olympus, but only have contacts for Sakura and Leica. If there are any sales people looking at this for the Wyoming area, please send information to me at the address at the bottom of this message. Also, for the tech's reading this. What in your opinion is the best (all areas) manual microtome out there? I currently have a Microm Ergostar and hate it. I have spent thousands of dollars trying to get it to not do the venetian blinding on 95% of our blocks, but it still does it. It doesn't make any difference as to what type os tissue I cut or the size. Thanks in advance, Daryl A. Mikita, HT(ASCP)cm Wyoming Medical Center Anatomical Pathology 1233 E. 2nd St. Casper, WY 82601 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Fri Jul 28 02:05:31 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Jul 28 02:05:32 2006 Subject: AW: [Histonet] How to stain frozen section with Gomori trichrome? In-Reply-To: <44C8CFF5020000F000004ABD@swnw124.swmed.edu> Message-ID: <001501c6b214$36e9f470$eeeea8c0@SERVER01> Try to shorten the staining time. The fiber-stain (blue) is likely to overstain the whole section, when incubation time is too long. We stained 8 min on FFPE sections. I think frozens stain faster overall. FFPE-staining without mordanting in Bouin rendered the cytoplasma blue. I don't know, how frozens behave in this manner. Hope this helps (a little bit) Gudrun -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Xilong Li Gesendet: Donnerstag, 27. Juli 2006 21:39 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] How to stain frozen section with Gomori trichrome? Hi, All members, I am a new member for histonet. I'm looking for a consistent protocol for a Gomori trichrome stain for frozen section. I suppose this topic was discussed previously, but I can not get exact answer when I try to review early messages. So I send messages here. I tried to stain frozen muscle section with gomori trichrome but failed recently. My protocol is as follow in brief: 1 frozen section was brought to room temperature before staining which was token from -80C. 2 stain nuclei with Weigert's Iron haematoxylin (sigma) for 15min 3 wash in distilled water for 1min 4 stain in staining solution (Chromotrope 2R 0.6g, fast green FCF 0.3g, Phosphotungstic acid 0.6g, Glacial acetic acid 1.0ml, distilled water 100ml, adjust pH to 3.4) for 12min 5 rinse in 1%(50ul/50ml) acetic acid in dips 6 dehydration with 100% alcohols in dips 7 put section in xylene 1min 8 mount The pictures was so bad without the red color for cytoplasma, almost all part of muscle tissue or cytoplasma was green or dark(which suppose to be red color), it was so hard to make clear where is collagen, or nuclei which was stained in funny red color at some extent. I changed formula of one step stain solution (Chromotrope 2R 0.6g, fast green FCF 0.1g, Phosphotungstic acid 0.8g, Glacial acetic acid 1.0ml, distilled water 100ml, adjust pH to 3.4) according to sigma formula of commercially prepared solution, and stained again, no improvement was gotten. So I am just looking forward to hearing from any guys, who can give me some suggestions what was wrong with my current protocol, and how to improve it, or just email me the protocol which works in their labs. My email is: xilong.li@utsouthwestern.edu, or just fax me 214-648-7902. Thanks in advance. Dr. Xilong Li Hypertension Division, Internal Medicine University of Texas Southwestern Medical Center 5323 Harry Hiness Blvd-J4.142 Dallas, TX 75390 Tel: 214-648-9966(L) Fax: 214-648-7902 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From vanann702 <@t> skmc.gov.ae Fri Jul 28 02:12:52 2006 From: vanann702 <@t> skmc.gov.ae (Anne Van Binsbergen) Date: Fri Jul 28 02:11:06 2006 Subject: [Histonet] How to stain frozen section with Gomori trichrome? References: <44C8CFF5020000F000004ABD@swnw124.swmed.edu> Message-ID: Hi my method worked for years on muscle - try harris or gills hx for 10mins instead of the weigerts - all other steps the same. it should work Annie ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Xilong Li Sent: Thu 2006/07/27 11:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How to stain frozen section with Gomori trichrome? Hi, All members, I am a new member for histonet. I'm looking for a consistent protocol for a Gomori trichrome stain for frozen section. I suppose this topic was discussed previously, but I can not get exact answer when I try to review early messages. So I send messages here. I tried to stain frozen muscle section with gomori trichrome but failed recently. My protocol is as follow in brief: 1 frozen section was brought to room temperature before staining which was token from -80C. 2 stain nuclei with Weigert's Iron haematoxylin (sigma) for 15min 3 wash in distilled water for 1min 4 stain in staining solution (Chromotrope 2R 0.6g, fast green FCF 0.3g, Phosphotungstic acid 0.6g, Glacial acetic acid 1.0ml, distilled water 100ml, adjust pH to 3.4) for 12min 5 rinse in 1%(50ul/50ml) acetic acid in dips 6 dehydration with 100% alcohols in dips 7 put section in xylene 1min 8 mount The pictures was so bad without the red color for cytoplasma, almost all part of muscle tissue or cytoplasma was green or dark(which suppose to be red color), it was so hard to make clear where is collagen, or nuclei which was stained in funny red color at some extent. I changed formula of one step stain solution (Chromotrope 2R 0.6g, fast green FCF 0.1g, Phosphotungstic acid 0.8g, Glacial acetic acid 1.0ml, distilled water 100ml, adjust pH to 3.4) according to sigma formula of commercially prepared solution, and stained again, no improvement was gotten. So I am just looking forward to hearing from any guys, who can give me some suggestions what was wrong with my current protocol, and how to improve it, or just email me the protocol which works in their labs. My email is: xilong.li@utsouthwestern.edu, or just fax me 214-648-7902. Thanks in advance. Dr. Xilong Li Hypertension Division, Internal Medicine University of Texas Southwestern Medical Center 5323 Harry Hiness Blvd-J4.142 Dallas, TX 75390 Tel: 214-648-9966(L) Fax: 214-648-7902 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lpwenk <@t> sbcglobal.net Fri Jul 28 04:40:36 2006 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Fri Jul 28 04:41:30 2006 Subject: [Histonet] How to stain frozen section with Gomori trichrome? In-Reply-To: <44C8CFF5020000F000004ABD@swnw124.swmed.edu> Message-ID: <000901c6b229$e1f941d0$1b364944@HPPav2> Welcome to the wonderful world of Histonet! On a frozen section of muscle, this modified Gomori trichrome IS supposed to have green muscle with slightly lighter green connective tissue. What is supposed to be red are the mitochondria and the nemaline rods, which, in normal muscle, you wouldn't see. (On higher power, you might see very small red dots in the muscle fibers, which are the mitochondria.) However, in cases of mitochondrial diseases, where there is an increase number of mitochondria, such as a condition known as red-ragged fibers, then you will see the accumulated mitochondria. Also, nemaline myopathies would have an increase in nemaline rods, and these would be seen as red. Plus, normal nerve fibers will have some red in them, so if you have a section of larger nerve in the muscle connective tissue, you would see red. Try going to Google, and right above the rectangle where you type in the key words, click on "Images" (it's usually defaulted to "Web". Then type in "Gomori trichrome muscle" (without the ") in the box and hit Search. You will see lots of images, all green on green, with a few showing the red-ragged fibers. Or type in "red ragged fiber" or "nemaline rod", if you want to see the modified Gomori trichrome in these diseases. Comparison of procedures: Usual Gomori Trichrome - Uses fixed, processed tissue, with the sections post-mordanted in Bouins, and the pH of the Gomori Trichrome staining solution between 1.5-2.5 pH, THEN the muscles are red and the connective tissue green. Modified Gomori Trichrome for muscle - Procedure is modified for FS muscle (tissue is not fixed, not processed, not post-mordanted in Bouins, and the staining solution is at pH 3.4) specifically so it WILL demonstrate mitochondria and nemaline rods as red, NOT to differentiate muscle from connective tissue. A really good site is the Neuromuscular Disease Center from Washington University in St. Louis, MO. http://www.neuro.wustl.edu/neuromuscular/ Hope this helps. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Xilong Li Sent: Thursday, July 27, 2006 3:39 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How to stain frozen section with Gomori trichrome? Hi, All members, I am a new member for histonet. I'm looking for a consistent protocol for a Gomori trichrome stain for frozen section. I suppose this topic was discussed previously, but I can not get exact answer when I try to review early messages. So I send messages here. I tried to stain frozen muscle section with gomori trichrome but failed recently. My protocol is as follow in brief: 1 frozen section was brought to room temperature before staining which was token from -80C. 2 stain nuclei with Weigert's Iron haematoxylin (sigma) for 15min 3 wash in distilled water for 1min 4 stain in staining solution (Chromotrope 2R 0.6g, fast green FCF 0.3g, Phosphotungstic acid 0.6g, Glacial acetic acid 1.0ml, distilled water 100ml, adjust pH to 3.4) for 12min 5 rinse in 1%(50ul/50ml) acetic acid in dips 6 dehydration with 100% alcohols in dips 7 put section in xylene 1min 8 mount The pictures was so bad without the red color for cytoplasma, almost all part of muscle tissue or cytoplasma was green or dark(which suppose to be red color), it was so hard to make clear where is collagen, or nuclei which was stained in funny red color at some extent. I changed formula of one step stain solution (Chromotrope 2R 0.6g, fast green FCF 0.1g, Phosphotungstic acid 0.8g, Glacial acetic acid 1.0ml, distilled water 100ml, adjust pH to 3.4) according to sigma formula of commercially prepared solution, and stained again, no improvement was gotten. So I am just looking forward to hearing from any guys, who can give me some suggestions what was wrong with my current protocol, and how to improve it, or just email me the protocol which works in their labs. My email is: xilong.li@utsouthwestern.edu, or just fax me 214-648-7902. Thanks in advance. Dr. Xilong Li Hypertension Division, Internal Medicine University of Texas Southwestern Medical Center 5323 Harry Hiness Blvd-J4.142 Dallas, TX 75390 Tel: 214-648-9966(L) Fax: 214-648-7902 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From MDiCarlo <@t> KaleidaHealth.Org Fri Jul 28 08:46:08 2006 From: MDiCarlo <@t> KaleidaHealth.Org (DiCarlo, Margaret) Date: Fri Jul 28 08:46:18 2006 Subject: [Histonet] x-ray ruler Message-ID: <9B4A77DF11463E4FB723D484214AE9BC0128B275@KALEXMB02.KaleidaHealth.org> Histonetters, Can anyone tell me where I can purchase a ruler used for x-raying bone specimens? Mine is old, broken and warped; it's time to replace it. I looked in a couple of catalogs but was unsuccessful. Any ideas where I can find one? Thanks for your help. Peggy DiCarlo HT(ASCP) Orthopedic Bone Lab Buffalo General Hospital 100 High St. Buffalo, NY 14203 716-859-1293 CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From caron_fournier <@t> yahoo.ca Fri Jul 28 08:47:19 2006 From: caron_fournier <@t> yahoo.ca (caron fournier) Date: Fri Jul 28 08:47:23 2006 Subject: [Histonet] texas red imaging with fitc Message-ID: <20060728134719.37925.qmail@web35411.mail.mud.yahoo.com> Just wanted to comment to Michelle that what Jim says is correct but spectral unmixing is not the only way to get around this. That is one way but is also the most expensive. The best bet is to make sure you have the right filter cubes for the dyes you are using and that they have a very steep cut off so that you can stop the long pass emission that they fitc has from exciting the texas red. Caron Caron Fournier, BSc, R.T. Department of Orthopaedics, Division of Orthopaedic Engineering Research, U.B.C. --------------------------------- The best gets better. See why everyone is raving about the All-new Yahoo! Mail. From kweidenh <@t> montefiore.org Fri Jul 28 09:01:10 2006 From: kweidenh <@t> montefiore.org (Karen Weidenheim) Date: Fri Jul 28 09:01:36 2006 Subject: [Histonet] Electron Microscopy of Cilia Message-ID: I need to look at cilia from the respiratory tract in patients with repeated sinus infections and pneumonia by electron microscopy, to see if the microtubules and associated protein linkages are okay. I have been staining them with lead citrate and uranyl acetate but while the microtubules are well stained and crisp, several cases have fuzzy radial spokes and nexin links. Is this pathology or do I need to modify my staining or could these tiny structures be autolysing faster than the microtubules? I would be grateful if anyone knows anything about this very specialized subject. Thanks Karen Karen M. Weidenheim, M.D. Professor of Pathology, Clinical Neurology and Clinical Neurosurgery Albert Einstein College of Medicine Chief, Division of Neuropathology Montefiore Medical Center 111 East 210th Street Bronx, NY 10467 (718) 920-4446 FAX (718) 653-3409 Beeper (917) 556 3696 CONFIDENTIALITY NOTICE: This email, including any attachments, is for the sole use of the intended recipient(s). The information contained in this message may be private and confidential, and may also be subject to the work product doctrine. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. Thank you. From MDiCarlo <@t> KaleidaHealth.Org Fri Jul 28 09:22:26 2006 From: MDiCarlo <@t> KaleidaHealth.Org (DiCarlo, Margaret) Date: Fri Jul 28 09:22:32 2006 Subject: [Histonet] x-ray ruler Message-ID: <9B4A77DF11463E4FB723D484214AE9BC0128B289@KALEXMB02.KaleidaHealth.org> Histonetters, Can anyone tell me where I can purchase a ruler used for x-raying bone specimens? Mine is old, broken and warped; it's time to replace it. I looked in a couple of catalogs but was unsuccessful. Any ideas where I can find one? Thanks for your help. Peggy DiCarlo HT(ASCP) Orthopedic Bone Lab Buffalo General Hospital 100 High St. Buffalo, NY 14203 716-859-1293 CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From gentras <@t> vetmed.auburn.edu Fri Jul 28 10:11:14 2006 From: gentras <@t> vetmed.auburn.edu (Atoska S. Gentry) Date: Fri Jul 28 10:11:25 2006 Subject: [Histonet] Tris buffer Message-ID: <7.0.1.0.0.20060728100524.01818730@vetmed.auburn.edu> Hello, a colleague of mine is in search of a Tris buffer that's appropriate to titrate the pH of both 30% and 20% CaCl2 ( Calcium Chloride). Has anyone a source for the concentration of the buffer needed? Also, should she use Trizma - Base alone or a combination of Trizma -Base & Trizma - HCl. Your prompt replies will be much appreciated. Thanks, Atoska From failm <@t> musc.edu Fri Jul 28 10:56:46 2006 From: failm <@t> musc.edu (Mildred Fail) Date: Fri Jul 28 10:59:11 2006 Subject: [Histonet] anti-fade queous mounting media Message-ID: I need an anti-fade aqueous mounting media for coverslipping fluorescent slides. The pathologist has specifically requested that it contain phenylenediamine. Vendors responses welcome Thank you in advance for your help Rena Fail From gcallis <@t> montana.edu Fri Jul 28 11:12:27 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Jul 28 11:12:37 2006 Subject: [Histonet] Doing double immunofluorescence staining on bacteria, a dilemma, long message Message-ID: <6.0.0.22.1.20060728095228.01b40358@gemini.msu.montana.edu> I have a frustrated researcher whose post doc is trying to stain for a protein (antigen) inside the bacteria and a surface antigen. e.g. double immunofluorescence. We know the antibodies work and will stain both, but in different circumstances. The dilemma is 1. Internalized antigen stains after acetone and acetone/alcohol fixed bacteria but surface antigen does not - Tween 20 was added to buffer to aid permeabilization. 2. On unfixed bacteria, internalized antigen does not stain, surface antigen stains - no detergents are used in buffers. Obviously the acetone/alcohol fixation aided in permeabilization but must have damage the surface antigen. Some antigens do NOT like solvents. We suspect the surface antigen is compromised by either fixation, detergent or both. The bacterial cell is a lipopolysaccharide in nature, and not sure saponin will be much help here either since it is for the cholesterol component of cell walls/membranes. ******************************************************************************************************************* In the past, and before Histonet archives were set up (thank heavens for keeping hard copies!) I had this information sent by Peter van de Plas, from Aurion in Sept 1998. He sent this excellent reply to question about diffferences between Triton and Tween detergents. "Both Tween and Triton are non-ionic detergents. Triton is a low molecular weight detergent with high capacity to solubilize lipids/lipid like structures. Even after aldehyde fixation you will lose most of your membranes and membrane bound proteins. I would therefore not use it in an immunoincubation procedure (you may wash out your antigen. Tween is a much milder detergent. It is normally used above the critical micell concentration of 0.07%. the Tween micells will encapsulate antibodies and other proteins used in the incubation procedure thus preventing them to bind specifically to hydrophobic (sticky) sites in your specimen. It reduces the background by suppressing ctivity of e. g. BSA, normal serums, etc. Like a soap, it will wash all loosely bound protein and it may wash out part of your antigen." Peter van de Plas Aurion (a company who sells reagents for immunogold staining) ******************************************************************************************************************************* I guess one alternative is to have the internalized protein expressing eGFP, and do unfixed bacteria IFA staining for the surface antigen, a nice way to eliminate permeabilization and fixation. Are there any other ways to permeabilize bacterial cell walls? I don't think the researcher is to enamored with trying paraformaldehyde fixation if it involves retrievals, etc on these bacteria. I am not doing the staining (my beloved CD markers in frozen sections are easy by comparison!). Any suggestions will be welcomed. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From gcallis <@t> montana.edu Fri Jul 28 11:18:54 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Jul 28 11:19:02 2006 Subject: [Histonet] anti-fade queous mounting media In-Reply-To: References: Message-ID: <6.0.0.22.1.20060728101314.01b73a78@gemini.msu.montana.edu> Try Molecular Probes Prolong Gold antifade, it comes in hard set but follow brochure directions for use. I don't know what it contains, but it works, sections can be coverslipped and if stored properly, still fluoresce over time. It will also depend on fluorophore, Alexa 610 cannot be coverslipped with this mounting media. Hard sets should be sealed. Once again it will depend on if you are working with eGFP, avoid fingernail polish and use xylene or toluene based mounting media around coverslip edges, but dilute the media so you can paint it on. If using standard fluorophores, fluroeceinated, Alexas or Cy fluorphores, then fingernail polish works fine. Also, Vector Vectashield is antifade, hard set - you can also get this in a non- hard set media. Both of these mounting medias come with or without DAPI. At 09:56 AM 7/28/2006, you wrote: >I need an anti-fade aqueous mounting media for coverslipping fluorescent >slides. The pathologist has specifically requested that it contain >phenylenediamine. Vendors responses welcome >Thank you in advance for your help > >Rena Fail > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From osullivan <@t> mpih-frankfurt.mpg.de Fri Jul 28 11:46:26 2006 From: osullivan <@t> mpih-frankfurt.mpg.de (Gregory A. O'Sullivan) Date: Fri Jul 28 11:46:39 2006 Subject: [Histonet] Antibody staining on non-pfa fixed tissue Message-ID: <01ca01c6b265$5ec779d0$d205058d@MPIHFrankfurt.mpg.de> Hello HistoNetters, I realise that this may be a question that has arisen on previous occasions, however, I have been unable to find an exact answer from a search of previous email correspondence on HistoNet. I would like to detect and examine the distribution of my protein of interest in mouse brain sections (with particular emphasis on the hippocampus) and compare it to other known markers. The problem is that the antibody I am using is extremely sensitive to pfa fixation but the marker antigens are best detected on fixed tissue. I have tried to overcome this problem by fixing cryostat sections briefly with pfa before using different antigen retrieval protocols (either microwave or heated bath incubation in Citrate Bufer) to unmask epitopes but this results in a staining that is suboptimal for both antigens (protein of interest and marker). One 'idea' would be to try and incubate these dried crystat section with my antibody of interest and then post fix with pfa before proceeding with the marker antibody incubation. My concern here is that these unfixed sections will undergo degradation prior to fixation but perhaps someone might have experience with regard to this. An alternative, I have come across from previous emails, is to fix the tissue by dipping the sections in acetone for 1 to 2 minutes at rtp. I was wondering whether anyone has any experience of using such a protocol for antibodies that are pfa fixation sensitive? Anyhow, any suggestions would be extremely helpful. Greg Gregory A. O'Sullivan PhD MPI for Brain Research Deutschordenstrasse 46 60528 Frankfurt Deutschland Tel: +49 69 96769315 Fax: +49 69 96769441 Mob: +49 163 6359371 email: osullivan@mpih-frankfurt.mpg.de From cpomajzl <@t> cpllabs.com Fri Jul 28 12:31:12 2006 From: cpomajzl <@t> cpllabs.com (Chris Pomajzl) Date: Fri Jul 28 12:26:02 2006 Subject: [Histonet] What kind of beer will you be drinking tonight? Message-ID: <004301c6b26b$9f927c20$26fca8c0@CSP> For me... I'll be picking up some ice cold Spaten on my way home today. TGIF!!! From jqb7 <@t> cdc.gov Fri Jul 28 12:52:04 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/NCID/VR)) Date: Fri Jul 28 12:56:57 2006 Subject: [Histonet] What kind of beer will you be drinking tonight? References: <004301c6b26b$9f927c20$26fca8c0@CSP> Message-ID: I'll be having a freezer-cold Chopin martini! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Chris Pomajzl Sent: Fri 7/28/2006 1:31 PM To: HISTONET Cc: Subject: [Histonet] What kind of beer will you be drinking tonight? For me... I'll be picking up some ice cold Spaten on my way home today. TGIF!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From NMHisto <@t> aol.com Wed Jul 26 16:34:15 2006 From: NMHisto <@t> aol.com (NMHisto@aol.com) Date: Fri Jul 28 12:58:04 2006 Subject: [Histonet] NEW MEXICO SOCIETY FOR HISTOLOGY Message-ID: <566.2a01e43.31f939d7@aol.com> It appears that the New Mexico Society for Histology will be hosting the NMSH Annual Meeting October 21, 2006 in Albuquerque! Plans are underway for a day-long meeting at the Sheraton Albuquerque Uptown. Histologists, histotechnologists, histologists-in-progress, those-interested-in-histology, fans-of-histology, pathologists-who-love-histologists, and recently-retired histologists are urged to contact me as soon as possible to be added to the mailing list and receive more information about this long-awaited meeting! The same invitation is extended to cytologists within our viewing area - come and join us! Our beloved vendors are invited to be in contact to see how we can showcase your products at our little gathering. Details will be forthcoming as we progress. Thanks for the chance to post this exciting little message! Sally Breeden President, NMSH Email: _nmhisto@aol.com_ (mailto:nmhisto@aol.com) Email: _sbreeden@nmda.nmsu.edu_ (mailto:sbreeden@nmda.nmsu.edu) From rhoglan <@t> harrisonmedical.org Fri Jul 28 13:05:25 2006 From: rhoglan <@t> harrisonmedical.org (Renee Hoglan) Date: Fri Jul 28 13:05:55 2006 Subject: [Histonet] (no subject) Message-ID: <44C9EF75020000460000153F@HMHDMZ.HMHWEB.ORG> Does anyone have any suggestions to get nail bed samples to stick to the slides without having the sample fall off in the staining process? From tkngflght <@t> yahoo.com Fri Jul 28 13:55:02 2006 From: tkngflght <@t> yahoo.com (Cheryl) Date: Fri Jul 28 13:55:05 2006 Subject: [Histonet] What kind of beer will you be drinking tonight? In-Reply-To: <004301c6b26b$9f927c20$26fca8c0@CSP> Message-ID: <20060728185502.61966.qmail@web50903.mail.yahoo.com> If anyone on this histonet would volunteer to ship me a case of Redhook's porter (called Blackhook), I'd be grateful and drink to your continued good health. I'd pay for the beer, the shipping and willingly share...any one in the Pacific Northwest willing to help a histotech in need?? Cheryl Full Staff Houston TX Chris Pomajzl wrote: For me... I'll be picking up some ice cold Spaten on my way home today. TGIF!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From psicurello <@t> mcvh-vcu.edu Fri Jul 28 14:24:47 2006 From: psicurello <@t> mcvh-vcu.edu (Paula Sicurello) Date: Fri Jul 28 14:26:26 2006 Subject: [Histonet] Electron Microscopy of Cilia Message-ID: The fuzziness has to do with the plane of sec tilt the sections, you can do this if the TEM ha Only if you're really lucky can you get a perfect cut axis of the cilia. Paula :-) From Traczyk7 <@t> aol.com Fri Jul 28 14:41:13 2006 From: Traczyk7 <@t> aol.com (Traczyk7@aol.com) Date: Fri Jul 28 14:41:20 2006 Subject: [Histonet] Looking for Pam Hesch Message-ID: <4fa.3d77aea.31fbc259@aol.com> Pam, are you out there? Give me a call when you get the chance. Regards, Dorothy Hacker Instruments Inc. 800-442-2537 x11 From funderwood <@t> mcohio.org Fri Jul 28 14:48:41 2006 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Fri Jul 28 14:50:12 2006 Subject: [Histonet] What kind of beer will you be drinking tonight? Message-ID: ahhh. I'll probably get the weekend rolling with an Arrogant Bastard. >>> "Chris Pomajzl" 07/28 1:31 PM >>> For me... I'll be picking up some ice cold Spaten on my way home today. TGIF!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DavidH <@t> marketlabinc.com Fri Jul 28 15:25:09 2006 From: DavidH <@t> marketlabinc.com (David Haagsma) Date: Fri Jul 28 15:25:15 2006 Subject: [Histonet] What kind of beer will you be drinking tonight? Message-ID: <19E3602A16438E48B51A4250CA04B5F6782BD6@exchange.marketlab.com> Oberon from Bell's Brewery - It's darn hot and beer sounds Great! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fred Underwood Sent: Friday, July 28, 2006 3:49 PM To: cpomajzl@cpllabs.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] What kind of beer will you be drinking tonight? ahhh. I'll probably get the weekend rolling with an Arrogant Bastard. >>> "Chris Pomajzl" 07/28 1:31 PM >>> For me... I'll be picking up some ice cold Spaten on my way home today. TGIF!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jqb7 <@t> cdc.gov Fri Jul 28 15:36:39 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/NCID/VR)) Date: Fri Jul 28 15:37:28 2006 Subject: [Histonet] What kind of beer will you be drinking tonight? References: Message-ID: I htink I work with a couple of those. :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Fred Underwood Sent: Fri 7/28/2006 3:48 PM To: cpomajzl@cpllabs.com; histonet@lists.utsouthwestern.edu Cc: Subject: Re: [Histonet] What kind of beer will you be drinking tonight? ahhh. I'll probably get the weekend rolling with an Arrogant Bastard. >>> "Chris Pomajzl" 07/28 1:31 PM >>> For me... I'll be picking up some ice cold Spaten on my way home today. TGIF!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From c.ingles <@t> hosp.wisc.edu Fri Jul 28 17:04:27 2006 From: c.ingles <@t> hosp.wisc.edu (Ingles Claire) Date: Fri Jul 28 17:04:31 2006 Subject: [Histonet] What kind of beer will you be drinking tonight? In-Reply-To: <19E3602A16438E48B51A4250CA04B5F6782BD6@exchange.marketlab.com> References: <19E3602A16438E48B51A4250CA04B5F6782BD6@exchange.marketlab.com> Message-ID: <9DBB068A-255D-4482-8138-F27A3FF430AB@mimectl> Gee, and I thought we worked with enough alcohol during the week! ;) Claire From: David Haagsma Sent: Fri 7/28/2006 3:25 PM To: Fred Underwood; cpomajzl@cpllabs.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] What kind of beer will you be drinking tonight? Oberon from Bell's Brewery - It's darn hot and beer sounds Great! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fred Underwood Sent: Friday, July 28, 2006 3:49 PM To: cpomajzl@cpllabs.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] What kind of beer will you be drinking tonight? ahhh. I'll probably get the weekend rolling with an Arrogant Bastard. >>> "Chris Pomajzl" 07/28 1:31 PM >>> For me... I'll be picking up some ice cold Spaten on my way home today. TGIF!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tahseen <@t> brain.net.pk Fri Jul 28 17:40:39 2006 From: tahseen <@t> brain.net.pk (Tahseen) Date: Fri Jul 28 17:40:53 2006 Subject: [Histonet] Histology workload standards References: <270614B321ACB44D8C1D91F4F921FDC3036CBE91@NCH01EX02.nch.org> Message-ID: <00a501c6b296$db095be0$7a1e80cb@p> Dear Laura Galiotto, See the attach file for Histology workload, which we are using over section. Muhammad Tahseen Histology Supervisor, SKMCH&RC Lahore,Pakistan. ----- Original Message ----- From: "Galiotto, Laura" To: Sent: Wednesday, July 26, 2006 12:13 AM Subject: [Histonet] Histology workload standards Hello Histotechs Can anyone tell me if they are aware of standards set by any governing body for Histology workload? I have contacted CAP and ASCP without success. I am having difficulties connecting CLIA. Also if you would be so kind and tell me how many techs you have for every 5000 -10000 cases per year. Laura Galiotto, HT (ASCP) Histology Facilitator ******************* PLEASE NOTE ******************* This E-Mail/telefax message and any documents accompanying this transmission may contain information that is privileged, confidential, and/or exempt from disclosure under applicable law and is intended solely for the addressee(s) named above. If you are not the intended addressee/recipient, you are hereby notified that any use of, disclosure, copying, distribution, or reliance on the contents of this E-Mail/telefax information is strictly prohibited and may result in legal action against you. Please reply to the sender advising of the error in transmission and immediately delete/destroy the message and any accompanying documents. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tahseen <@t> brain.net.pk Fri Jul 28 17:47:44 2006 From: tahseen <@t> brain.net.pk (Tahseen) Date: Fri Jul 28 17:47:48 2006 Subject: [Histonet] (no subject) References: <44C9EF75020000460000153F@HMHDMZ.HMHWEB.ORG> Message-ID: <00c101c6b297$d7aa35e0$7a1e80cb@p> Mounted on superfrost (R) plus slides Menzel. Muhammad Tahseen ----- Original Message ----- From: "Renee Hoglan" To: Sent: Friday, July 28, 2006 11:05 PM Subject: [Histonet] (no subject) > Does anyone have any suggestions to get nail bed samples to stick to the > slides without having the sample fall off in the staining process? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Eric <@t> ategra.com Fri Jul 28 19:03:16 2006 From: Eric <@t> ategra.com (Eric Dye (ext 223)) Date: Fri Jul 28 22:06:26 2006 Subject: [Histonet] Nationwide Histology openings, interviewing and hiring now Message-ID: Hi - Fellow-Histonetters Are you happy in your current position? Below is the updated list of Histology j o b s, All openings are Dayshift Monday thru Friday unless indicated otherwise. All of these jobs are looking to move quickly so if your interested call me A S A P... If you are interested in any of the Histology jobs listed below or anywhere else - please call me at 800-466-9919 ext 223 or cell - 407-756-5507. Most HistoTech j o b s are permanent full-time Here are some of my Newest Histology Jobs: ------------------------------------------------------ Washington, D.C - Histotech -perm and temp( 3-6 months) New York (Long Island) - HistoTech - perm Northern New Jersey - HistoTech and Cyto Tech - perm Boston Mass - HistoTech - one Senior HistoTech, One not so Senior Histotech Massachusetts (North of Boston) - perm - Bench Histotech Ohio (Central) - one temp - Bench Histotech Minnesota (Twin Cities area - perm - Bench Histotech - Biotech/research - Make your own schedule!!! Southeast Florida - perm - HistoTech Southwest Florida - perm - Bench Histotech/ Supervisor Florida, West Coast - both temp & perm openings- Bench Histotech New Hampshire perm openings - Bench Histotech (opportunity to learn Mohs!) South Carolina - Perm - Supervisor and Bench - Histotech ---- end list of HistoTech Opportunities --- If you are interested in any of the Histology jobs listed above - please call me at 800-466-9919 ext 223 or cell - 407-756-5507. Thank you, Eric Dye-Sr Allied Healthcare Recruiter 800-466-9919 ext 223 or Cell - 407-756-5507 P.S.: Feel free to pass along this email and my phone number to anyone who you think might be interested. P.S.S.: The clients are currently interviewing - and the job will close soon - so if you are interested, please call me today at 1-800-466-9919 ext 229 --------------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing 800 466 9919 ext 223 - voice 407 671 6075 - fax [1]eric@ategra.com To Learn More About Ategra: [2]http://www.ategra.com ---------------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. ---------------------------------------------------------------- ---------------------------------------------------------------- Note: this message is intended for: Fellow-Histonetters at histonet@lists.utsouthwestern.edu ---------------------------------------------------------------- References 1. mailto:eric@ategra.com 2. http://www.ategra.com/ From c.m.vanderloos <@t> amc.uva.nl Mon Jul 31 02:56:27 2006 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Mon Jul 31 02:56:35 2006 Subject: [Histonet] RE: Antibody staining on non-pfa fixed tissue Message-ID: Dear Greg, As you already concluded the epitope of interest is PFA-sensitive. How about using a non-crosslinking fixative here? For example methacarn (methanol:chloroform:acetic aced = 6:3:1) does not contain formaldehydes. Fix your sample for 48 hours, dehydrate via alcohol series and embed in paraffin. Because methacarn is non-crosslinking fixative most antigens doesn't need antigen retrieval. Just a suggestion... Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Fri, 28 Jul 2006 18:46:26 +0200 From: "Gregory A. O'Sullivan" Subject: [Histonet] Antibody staining on non-pfa fixed tissue To: "HistoNet" Hello HistoNetters, I realise that this may be a question that has arisen on previous occasions, however, I have been unable to find an exact answer from a search of previous email correspondence on HistoNet. I would like to detect and examine the distribution of my protein of interest in mouse brain sections (with particular emphasis on the hippocampus) and compare it to other known markers. The problem is that the antibody I am using is extremely sensitive to pfa fixation but the marker antigens are best detected on fixed tissue. I have tried to overcome this problem by fixing cryostat sections briefly with pfa before using different antigen retrieval protocols (either microwave or heated bath incubatio! n in C From Jeannette.Mitchell <@t> vtmednet.org Mon Jul 31 05:40:01 2006 From: Jeannette.Mitchell <@t> vtmednet.org (Mitchell, Jeannette M.) Date: Mon Jul 31 05:40:07 2006 Subject: [Histonet] coverslipper Message-ID: Fletcher Allen Health Care Histology Laboratory has a Hacker Coverslipper, model RCM 3660 available to "best offer" for purchase. It is approx. 6 years old and is excellent working condition. We would keep but we have been allowed to lease a new coverslipper that has a smaller footprint and we have zero space to store the Hacker coverslipper as a back-up unit. Please call your offer/questions to: Jude Carpenter @ (802)847-5116 Or email: jude.carpenter@vtmednet.org Thank-you Jude Confidentiality Notice: This message, and any attachments, may contain information that is confidential, privileged, and/or protected from disclosure under state and federal laws that deal with the privacy and security of medical information. If you received this message in error or through inappropriate means, please reply to this message to notify the Sender that the message was received by you in error, and then permanently delete this message from all storage media, without forwarding or retaining a copy. From anh2006 <@t> med.cornell.edu Mon Jul 31 07:45:10 2006 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Mon Jul 31 07:45:24 2006 Subject: [Histonet] Ethanol/other fixations for frozen sections Message-ID: Just taking a survey .... What are everyone's thoughts on using ethanol for frozen sections? I have a colleague who swears by it but I am an acetone person myself. Thoughts, comments, input on what is the best fix for frozen section IHC? Thanks! -- From ryaskovich <@t> dir.nidcr.nih.gov Mon Jul 31 10:19:50 2006 From: ryaskovich <@t> dir.nidcr.nih.gov (Ruth Yaskovich) Date: Mon Jul 31 10:20:13 2006 Subject: [Histonet] RE: Antibody staining on non-pfa fixed tissue In-Reply-To: Message-ID: I have had good results with mouse and rat brain fixed in 70% Alcohol. Ruth Yaskovich National Institutes of Health National Institute of Dental and Craniofacial Research Pain and Neurosensory Mechanism Branch On 7/31/06 3:56 AM, "C.M. van der Loos" wrote: > > Dear Greg, > > As you already concluded the epitope of interest is PFA-sensitive. How > about using a non-crosslinking fixative here? For example methacarn > (methanol:chloroform:acetic aced = 6:3:1) does not contain > formaldehydes. Fix your sample for 48 hours, dehydrate via alcohol > series and embed in paraffin. Because methacarn is non-crosslinking > fixative most antigens doesn't need antigen retrieval. > > Just a suggestion... > > Chris van der Loos, PhD > Dept. of Pathology > Academic Medical Center M2-230 > Meibergdreef 9 > NL-1105 AZ Amsterdam > The Netherlands > > > Date: Fri, 28 Jul 2006 18:46:26 +0200 > From: "Gregory A. O'Sullivan" > Subject: [Histonet] Antibody staining on non-pfa fixed tissue > To: "HistoNet" > Hello HistoNetters, > I realise that this may be a question that has arisen on previous > occasions, however, I have been unable > to find an exact answer from a search of previous email correspondence > on HistoNet. > I would like to detect and examine the distribution of my protein of > interest in mouse brain sections > (with particular emphasis on the hippocampus) and compare it to other > known markers. > The problem is that the antibody I am using is extremely sensitive to > pfa fixation but the marker antigens > are best detected on fixed tissue. I have tried to overcome this > problem by fixing cryostat sections > briefly with pfa before using different antigen retrieval protocols > (either microwave or heated bath incubatio! n > in C > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From petepath <@t> yahoo.com Mon Jul 31 11:05:58 2006 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Mon Jul 31 11:06:02 2006 Subject: [Histonet] Ethanol/other fixations for frozen sections Message-ID: <20060731160558.22191.qmail@web30415.mail.mud.yahoo.com> Hi Andrea, I have always used 95% ETOH with excellent results. In hopes to expand my knowledge on fixatives I asked a similar question a few months ago. I had a number of kind responses and I mixed up and a number of the suggestions. I looked ethanol methanol alone and in various combination with formalin and acetic acid. Considering only morphology (as a surgical pathologist), ethanol and methanol alone and in the various combinations all gave beautiful preparations with excellent nuclear detail provided slides were fixed as quickly as possible after tissue is picked up with the slide. Formalin alone and acetone alone showed less nuclear detail.. From my reading acetic acid added to the mix should give less retraction. In my limited study I did not appreciate very much difference. The good thing is that all of these kind suggestions were quite excellent so it appeared to me that everyone was probably getting very good results with their coctails. AS I menion in my web site tutorial I think the most important factor is to fix the slide ASAP after it touches the slide because drying artifacts will considerably alter the morphology. Thank you to all of those who responded to my question. Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com From David.Edmondson <@t> christie-tr.nwest.nhs.uk Mon Jul 31 11:13:05 2006 From: David.Edmondson <@t> christie-tr.nwest.nhs.uk (Edmondson David (RBV) NHS Christie Tr) Date: Mon Jul 31 11:14:00 2006 Subject: [Histonet] Ethanol/other fixations for frozen sections Message-ID: <3C83687E8F6AE04792E361ABE2D385B8418170@cht-mail2-2k.xchristie.nhs.uk> Periodate-Lysine-Paraformaldehyde, may loose some staining but gain on morphology -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea T. Hooper Sent: 31 July 2006 13:45 To: Histonet Subject: [Histonet] Ethanol/other fixations for frozen sections Just taking a survey .... What are everyone's thoughts on using ethanol for frozen sections? I have a colleague who swears by it but I am an acetone person myself. Thoughts, comments, input on what is the best fix for frozen section IHC? Thanks! -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ****************************************************************************************************************** This e-mail and any files transmitted with it are confidential and solely for the use of the intended recipient. If you have received this e-mail in error you should not disseminate, distribute or copy it. Please notify the sender immediately and delete this e-mail from your system. ******************************************************************************************************************* From fawn <@t> cs.cmu.edu Mon Jul 31 11:18:38 2006 From: fawn <@t> cs.cmu.edu (Fawn Jones) Date: Mon Jul 31 11:18:42 2006 Subject: [Histonet] Help with Polycut Message-ID: <44CE2D5E.4000900@cs.cmu.edu> Is there anyone in the Pittsburgh or surrounding areas that use a Leica Polycut? I have one in my lab, but I have never used one or seen one in action and I was hoping that I may go somewhere to learn about it. Any help would be greatly appreciated. Thank you in advance Fawn Jones From GoodwinD <@t> pahosp.com Mon Jul 31 11:52:43 2006 From: GoodwinD <@t> pahosp.com (Goodwin, Diana) Date: Mon Jul 31 11:52:53 2006 Subject: [Histonet] RE: Histology workload standards Message-ID: <80CDD9C3FEEAFD4982B114C4A6DFD00E02CB800B@uphsmbx2.UPHS.PENNHEALTH.PRV> Laura: While it's true there are no established "benchmarks" per se for Histology lab productivity, there is a CAP Q-Probe (QP042) that you can participate in to compare your lab to others that are similar in workload and testing. Also, there was an article in the July 3 issue of the Advance for MLP entitled "Productivity in the Histology Lab" that presented data related to productivity. Hope this helps. Diana Goodwin Pennsylvania Hospital Philadelphia, PA -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Saturday, July 29, 2006 1:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 32, Issue 31 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. What kind of beer will you be drinking tonight? (Chris Pomajzl) 2. RE: What kind of beer will you be drinking tonight? (Bartlett, Jeanine (CDC/NCID/VR)) 3. NEW MEXICO SOCIETY FOR HISTOLOGY (NMHisto@aol.com) 4. (no subject) (Renee Hoglan) 5. Re: What kind of beer will you be drinking tonight? (Cheryl) 6. Re: Electron Microscopy of Cilia (Paula Sicurello) 7. Looking for Pam Hesch (Traczyk7@aol.com) 8. Re: What kind of beer will you be drinking tonight? (Fred Underwood) 9. RE: What kind of beer will you be drinking tonight? (David Haagsma) 10. RE: What kind of beer will you be drinking tonight? (Bartlett, Jeanine (CDC/NCID/VR)) 11. RE: What kind of beer will you be drinking tonight? (Ingles Claire) 12. Re: Histology workload standards (Tahseen) 13. Re: (no subject) (Tahseen) 14. Nationwide Histology openings, interviewing and hiring now (Eric Dye (ext 223)) ---------------------------------------------------------------------- Message: 1 Date: Fri, 28 Jul 2006 12:31:12 -0500 From: "Chris Pomajzl" Subject: [Histonet] What kind of beer will you be drinking tonight? To: "HISTONET" Message-ID: <004301c6b26b$9f927c20$26fca8c0@CSP> Content-Type: text/plain; charset="iso-8859-1" For me... I'll be picking up some ice cold Spaten on my way home today. TGIF!!! ------------------------------ Message: 2 Date: Fri, 28 Jul 2006 13:52:04 -0400 From: "Bartlett, Jeanine \(CDC/NCID/VR\)" Subject: RE: [Histonet] What kind of beer will you be drinking tonight? To: "Chris Pomajzl" , "HISTONET" Message-ID: Content-Type: text/plain; charset="utf-8" I'll be having a freezer-cold Chopin martini! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Chris Pomajzl Sent: Fri 7/28/2006 1:31 PM To: HISTONET Cc: Subject: [Histonet] What kind of beer will you be drinking tonight? For me... I'll be picking up some ice cold Spaten on my way home today. TGIF!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Wed, 26 Jul 2006 17:34:15 EDT From: NMHisto@aol.com Subject: [Histonet] NEW MEXICO SOCIETY FOR HISTOLOGY To: histonet@lists.utsouthwestern.edu Message-ID: <566.2a01e43.31f939d7@aol.com> Content-Type: text/plain; charset="US-ASCII" It appears that the New Mexico Society for Histology will be hosting the NMSH Annual Meeting October 21, 2006 in Albuquerque! Plans are underway for a day-long meeting at the Sheraton Albuquerque Uptown. Histologists, histotechnologists, histologists-in-progress, those-interested-in-histology, fans-of-histology, pathologists-who-love-histologists, and recently-retired histologists are urged to contact me as soon as possible to be added to the mailing list and receive more information about this long-awaited meeting! The same invitation is extended to cytologists within our viewing area - come and join us! Our beloved vendors are invited to be in contact to see how we can showcase your products at our little gathering. Details will be forthcoming as we progress. Thanks for the chance to post this exciting little message! Sally Breeden President, NMSH Email: _nmhisto@aol.com_ (mailto:nmhisto@aol.com) Email: _sbreeden@nmda.nmsu.edu_ (mailto:sbreeden@nmda.nmsu.edu) ------------------------------ Message: 4 Date: Fri, 28 Jul 2006 11:05:25 -0700 From: "Renee Hoglan" Subject: [Histonet] (no subject) To: Message-ID: <44C9EF75020000460000153F@HMHDMZ.HMHWEB.ORG> Content-Type: text/plain; charset=US-ASCII Does anyone have any suggestions to get nail bed samples to stick to the slides without having the sample fall off in the staining process? ------------------------------ Message: 5 Date: Fri, 28 Jul 2006 11:55:02 -0700 (PDT) From: Cheryl Subject: Re: [Histonet] What kind of beer will you be drinking tonight? To: HISTONET Message-ID: <20060728185502.61966.qmail@web50903.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 If anyone on this histonet would volunteer to ship me a case of Redhook's porter (called Blackhook), I'd be grateful and drink to your continued good health. I'd pay for the beer, the shipping and willingly share...any one in the Pacific Northwest willing to help a histotech in need?? Cheryl Full Staff Houston TX Chris Pomajzl wrote: For me... I'll be picking up some ice cold Spaten on my way home today. TGIF!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 6 Date: Fri, 28 Jul 2006 15:24:47 -0400 From: "Paula Sicurello" Subject: Re: [Histonet] Electron Microscopy of Cilia To: "Karen Weidenheim" Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="iso-8859-1" The fuzziness has to do with the plane of sec tilt the sections, you can do this if the TEM ha Only if you're really lucky can you get a perfect cut axis of the cilia. Paula :-) ------------------------------ Message: 7 Date: Fri, 28 Jul 2006 15:41:13 EDT From: Traczyk7@aol.com Subject: [Histonet] Looking for Pam Hesch To: histonet@lists.utsouthwestern.edu Message-ID: <4fa.3d77aea.31fbc259@aol.com> Content-Type: text/plain; charset="US-ASCII" Pam, are you out there? Give me a call when you get the chance. Regards, Dorothy Hacker Instruments Inc. 800-442-2537 x11 ------------------------------ Message: 8 Date: Fri, 28 Jul 2006 15:48:41 -0400 From: "Fred Underwood" Subject: Re: [Histonet] What kind of beer will you be drinking tonight? To: , Message-ID: Content-Type: text/plain; charset=US-ASCII ahhh. I'll probably get the weekend rolling with an Arrogant Bastard. >>> "Chris Pomajzl" 07/28 1:31 PM >>> For me... I'll be picking up some ice cold Spaten on my way home today. TGIF!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Fri, 28 Jul 2006 16:25:09 -0400 From: "David Haagsma" Subject: RE: [Histonet] What kind of beer will you be drinking tonight? To: "Fred Underwood" , , Message-ID: <19E3602A16438E48B51A4250CA04B5F6782BD6@exchange.marketlab.com> Content-Type: text/plain; charset="us-ascii" Oberon from Bell's Brewery - It's darn hot and beer sounds Great! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fred Underwood Sent: Friday, July 28, 2006 3:49 PM To: cpomajzl@cpllabs.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] What kind of beer will you be drinking tonight? ahhh. I'll probably get the weekend rolling with an Arrogant Bastard. >>> "Chris Pomajzl" 07/28 1:31 PM >>> For me... I'll be picking up some ice cold Spaten on my way home today. TGIF!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Fri, 28 Jul 2006 16:36:39 -0400 From: "Bartlett, Jeanine \(CDC/NCID/VR\)" Subject: RE: [Histonet] What kind of beer will you be drinking tonight? To: "Fred Underwood" , , Message-ID: Content-Type: text/plain; charset="utf-8" I htink I work with a couple of those. :) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Fred Underwood Sent: Fri 7/28/2006 3:48 PM To: cpomajzl@cpllabs.com; histonet@lists.utsouthwestern.edu Cc: Subject: Re: [Histonet] What kind of beer will you be drinking tonight? ahhh. I'll probably get the weekend rolling with an Arrogant Bastard. >>> "Chris Pomajzl" 07/28 1:31 PM >>> For me... I'll be picking up some ice cold Spaten on my way home today. TGIF!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Fri, 28 Jul 2006 17:04:27 -0500 From: Ingles Claire Subject: RE: [Histonet] What kind of beer will you be drinking tonight? To: Message-ID: <9DBB068A-255D-4482-8138-F27A3FF430AB@mimectl> Content-Type: text/plain; charset="iso-8859-1" Gee, and I thought we worked with enough alcohol during the week! ;) Claire From: David Haagsma Sent: Fri 7/28/2006 3:25 PM To: Fred Underwood; cpomajzl@cpllabs.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] What kind of beer will you be drinking tonight? Oberon from Bell's Brewery - It's darn hot and beer sounds Great! -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Fred Underwood Sent: Friday, July 28, 2006 3:49 PM To: cpomajzl@cpllabs.com; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] What kind of beer will you be drinking tonight? ahhh. I'll probably get the weekend rolling with an Arrogant Bastard. >>> "Chris Pomajzl" 07/28 1:31 PM >>> For me... I'll be picking up some ice cold Spaten on my way home today. TGIF!!! _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Sat, 29 Jul 2006 03:40:39 +0500 From: "Tahseen" Subject: Re: [Histonet] Histology workload standards To: "Galiotto, Laura" , Message-ID: <00a501c6b296$db095be0$7a1e80cb@p> Content-Type: text/plain; charset="iso-8859-1" Dear Laura Galiotto, See the attach file for Histology workload, which we are using over section. Muhammad Tahseen Histology Supervisor, SKMCH&RC Lahore,Pakistan. ----- Original Message ----- From: "Galiotto, Laura" To: Sent: Wednesday, July 26, 2006 12:13 AM Subject: [Histonet] Histology workload standards Hello Histotechs Can anyone tell me if they are aware of standards set by any governing body for Histology workload? I have contacted CAP and ASCP without success. I am having difficulties connecting CLIA. Also if you would be so kind and tell me how many techs you have for every 5000 -10000 cases per year. Laura Galiotto, HT (ASCP) Histology Facilitator ******************* PLEASE NOTE ******************* This E-Mail/telefax message and any documents accompanying this transmission may contain information that is privileged, confidential, and/or exempt from disclosure under applicable law and is intended solely for the addressee(s) named above. If you are not the intended addressee/recipient, you are hereby notified that any use of, disclosure, copying, distribution, or reliance on the contents of this E-Mail/telefax information is strictly prohibited and may result in legal action against you. Please reply to the sender advising of the error in transmission and immediately delete/destroy the message and any accompanying documents. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Sat, 29 Jul 2006 03:47:44 +0500 From: "Tahseen" Subject: Re: [Histonet] (no subject) To: "Renee Hoglan" , Message-ID: <00c101c6b297$d7aa35e0$7a1e80cb@p> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Mounted on superfrost (R) plus slides Menzel. Muhammad Tahseen ----- Original Message ----- From: "Renee Hoglan" To: Sent: Friday, July 28, 2006 11:05 PM Subject: [Histonet] (no subject) > Does anyone have any suggestions to get nail bed samples to stick to the > slides without having the sample fall off in the staining process? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Fri, 28 Jul 2006 20:03:16 -0400 From: Eric Dye (ext 223) Subject: [Histonet] Nationwide Histology openings, interviewing and hiring now To: Histonetters Message-ID: Content-Type: text/plain Hi - Fellow-Histonetters Are you happy in your current position? Below is the updated list of Histology j o b s, All openings are Dayshift Monday thru Friday unless indicated otherwise. All of these jobs are looking to move quickly so if your interested call me A S A P... If you are interested in any of the Histology jobs listed below or anywhere else - please call me at 800-466-9919 ext 223 or cell - 407-756-5507. Most HistoTech j o b s are permanent full-time Here are some of my Newest Histology Jobs: ------------------------------------------------------ Washington, D.C - Histotech -perm and temp( 3-6 months) New York (Long Island) - HistoTech - perm Northern New Jersey - HistoTech and Cyto Tech - perm Boston Mass - HistoTech - one Senior HistoTech, One not so Senior Histotech Massachusetts (North of Boston) - perm - Bench Histotech Ohio (Central) - one temp - Bench Histotech Minnesota (Twin Cities area - perm - Bench Histotech - Biotech/research - Make your own schedule!!! Southeast Florida - perm - HistoTech Southwest Florida - perm - Bench Histotech/ Supervisor Florida, West Coast - both temp & perm openings- Bench Histotech New Hampshire perm openings - Bench Histotech (opportunity to learn Mohs!) South Carolina - Perm - Supervisor and Bench - Histotech ---- end list of HistoTech Opportunities --- If you are interested in any of the Histology jobs listed above - please call me at 800-466-9919 ext 223 or cell - 407-756-5507. Thank you, Eric Dye-Sr Allied Healthcare Recruiter 800-466-9919 ext 223 or Cell - 407-756-5507 P.S.: Feel free to pass along this email and my phone number to anyone who you think might be interested. P.S.S.: The clients are currently interviewing - and the job will close soon - so if you are interested, please call me today at 1-800-466-9919 ext 229 --------------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing 800 466 9919 ext 223 - voice 407 671 6075 - fax [1]eric@ategra.com To Learn More About Ategra: [2]http://www.ategra.com ---------------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. ---------------------------------------------------------------- ---------------------------------------------------------------- Note: this message is intended for: Fellow-Histonetters at histonet@lists.utsouthwestern.edu ---------------------------------------------------------------- References 1. mailto:eric@ategra.com 2. http://www.ategra.com/ ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 32, Issue 31 **************************************** The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From AGrobe2555 <@t> aol.com Mon Jul 31 12:04:23 2006 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Mon Jul 31 12:04:37 2006 Subject: [Histonet] Beta-Amyloid Immunopreciptation Message-ID: Good Morning Folks, I was wondering if anyone has a protocol for the Immunoprecipitation of beta-amyloid that they would be willing to share? Thanks, Albert From sjchtascp <@t> yahoo.com Mon Jul 31 12:22:41 2006 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Mon Jul 31 12:22:44 2006 Subject: [Histonet] BrdU and GFP expression Message-ID: <20060731172241.65699.qmail@web38215.mail.mud.yahoo.com> Good afternoon from WI, We have several FFPE blocks from 2003 that express GFP. Our lead scientist would like to perform Brdu IHC in the same slides hoping to express both. As expected the AR removes the GFP green label. Has anyone any experience expressing Brdu and GFP on the same slide. Stay cool, Steve --------------------------------- Yahoo! Music Unlimited - Access over 1 million songs.Try it free. From MVaughan4 <@t> ucok.edu Mon Jul 31 12:28:52 2006 From: MVaughan4 <@t> ucok.edu (MVaughan4@ucok.edu) Date: Mon Jul 31 12:29:20 2006 Subject: [Histonet] Acetone for frozen sections and whole mounts In-Reply-To: Message-ID: My experience with acetone on frozen and whole mounts is that there is often nonspecific fluorescence in the connective tissues. This may not apply to enzyme IHC but can be bad for fluorescence. Try Ethanol or Methanol if this happens to you. Mel Melville B. Vaughan, Ph. D. Assistant Professor Department of Biology University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm From christopher.overend <@t> huskymail.uconn.edu Mon Jul 31 12:41:54 2006 From: christopher.overend <@t> huskymail.uconn.edu (Christopher C Overend) Date: Mon Jul 31 12:42:09 2006 Subject: [Histonet] IFA staining problems Message-ID: <1fbaf901fb5b0e.1fb5b0e1fbaf90@huskymail.uconn.edu> I am new to the technique of immunofluorescence, and have been doing some work with it lately on cultured cells. Specifically, I have been staining intracellular virus with very good results. However, when i try to detect cellular proteins, I do not get any staining. The proceedure i have been using consists of "fixation" with 90% acetone for 30 min, at 4degrees C. the rest of the process had been much like an ELISA, all incubations have been for 30 min at 4degrees. The buffer i have been using is PBS, 1%BSA, 0.1%NaN3. Someone mentioned the problem could be from the acetone and suggested trying a glyoxal fixative. Can anyone offer some insights, or if there is a different protocol i might have to follow using a glyoxal fixative with tissue culture? Thank you! Chris Christopher Overend Ph.D Student University of Connecticut Department of Pathobiology and Veterinary Sciences Storrs, CT Christopher.overend@uconn.edu From narangakhil <@t> yahoo.com Mon Jul 31 13:28:19 2006 From: narangakhil <@t> yahoo.com (Akhil Narang) Date: Mon Jul 31 13:28:23 2006 Subject: [Histonet] Immunohistochemistry with VEGF Message-ID: <20060731182819.19580.qmail@web50112.mail.yahoo.com> Hello all, I am a medical student working on a project and I was wondering if anyone could help me with an experiment I am trying to do. I am looking to stain a cell line for the presence of VEGF. I don't have any tissue samples - I'm just working with cells in culture. Does anyone have a recommended protocol or procedure to stain cells in suspension using VEGF antibody? Any help would be appreciated. Please e-mail me at: narangakhil@yahoo.com Thanks, Akhil --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail Beta. From bayoubelle311 <@t> gmail.com Mon Jul 31 13:47:11 2006 From: bayoubelle311 <@t> gmail.com (Missy) Date: Mon Jul 31 13:47:18 2006 Subject: [Histonet] Microwave decalcification Message-ID: <398f02c20607311147q3734c88bo670cbcebf0775be6@mail.gmail.com> Hi, I have a protocol that calls for a special tissue processor with microwaves, however I was curious if there are any protocols out there that utilize a standard microwave oven. I am trying to expedite the decalcification of rat femur, while maintaining the integrity of the fibrotic tissue in the bone marrow, which will later be quantified with gomori stain. Thanks, Melissa From ROENN <@t> surgery.wisc.edu Mon Jul 31 14:02:03 2006 From: ROENN <@t> surgery.wisc.edu (Drew Allan Roenneburg) Date: Mon Jul 31 14:02:40 2006 Subject: [Histonet] Foxp3 on Murine tissue Message-ID: <44CE0D5B0200009C00001F23@gw.surgery.wisc.edu> Hi, I was wondering if anyone has stained for foxp3 on frozen or FFPE mouse tissues (spleen). I have tried the Abcam rabbit anti-human foxp3 antibody that works on FFPE human and monkey (tonsil and spleen), however have had little success on mouse. Thanks Drew Roenneburg From jamie.erickson <@t> abbott.com Mon Jul 31 14:55:09 2006 From: jamie.erickson <@t> abbott.com (Jamie E Erickson) Date: Mon Jul 31 14:55:15 2006 Subject: [Histonet] Re: Ethanol/other fixations for frozen sections (Andrea T. Hooper) In-Reply-To: <55n6s0$ib4c9@cobra.abbott.com> Message-ID: Hi Andrea, Well it seems as if there are more then one answer to this question. Here is what I do. I got this suggestion from Gayle Callis and it works great for some not all markers. I section my frozen mouse tissues and set them to dry at room temp in a hood over night so they are bone dry before fixing. I've also done this after a few hours and it's just as good. The next day I fix in 75% acetone/ 25% 100% ethanol for 5 minutes at RT and wash 3x in TBS and go forward with IHC. C45/B220 works well this way, GL7 did not work well I saw diminished staining compared to acetone alone. I think Gayle used it on CD4/CD8 antibodies from BD. The morphology was better then acetone alone. You may have to test your antibody out before doing your real run. We had seen this with another reagent MORPHOSAVE, it was hit or miss with some antibodies. We also have done PFA fixed slide right after sectioning for 2 minutes then washed in dH20 2x then put in TBS for IHC. The morphology was great for our CXCL13 antibody from R&D. Hope it helps. Jamie _______________________________ Jamie Erickson Sr. Research Associate Department: DSMP Abbott Bioresearch Center 100 Research Drive Worcester, MA 01605-4341 508-688-3134 FAX: 508-793-4895 e-mail: jamie.erickson@abbott.com From abardo <@t> mcbaininstruments.com Mon Jul 31 19:39:24 2006 From: abardo <@t> mcbaininstruments.com (Ann Bardo) Date: Mon Jul 31 19:38:47 2006 Subject: [Histonet] Job Postings to Clinicians Message-ID: <200607311738296.SM01560@WSAnn> I previously submitted this job description for your consideration and posting on Histonet. I am submitting it again after filing out the subscriber information. I look forward to hearing from you and hopefully your acceptance of this posting. Thank you in advance for your assistance. Ann Bardo Executive Assistant to the CEO McBain Instruments 818-998-2702 www.mcbaininstruments.com From abardo <@t> mcbaininstruments.com Mon Jul 31 19:48:57 2006 From: abardo <@t> mcbaininstruments.com (Ann Bardo) Date: Mon Jul 31 19:48:19 2006 Subject: [Histonet] Position Available: Histology Sales Specialist - Southern California Message-ID: <200607311748421.SM01560@WSAnn> Histology Sales Specialist - Southern California Since 1965, McBain Instruments has supplied histology, microscopy and imaging solutions for health care, industrial, R&D and biomedical applications. Headquartered in Chatsworth, California, we are Leica Microsystems' Exclusive Regional Dealer in the Southwestern United States. We also offer numerous accessory product lines for digital imaging, systems automation, image analysis, and more. Job Title: Histology Sales Specialist - Orange, San Diego, Riverside, and San Bernardino Counties McBain Instruments is seeking a highly motivated, dynamic, results-oriented professional for the role of Histology Sales Specialist. Job Responsibilities include: * Generate profitable sales revenue * Achieve or exceed monthly, quarterly and annual sales targets * Plan and schedule face-to-face account calls to current and potential end-users of Leica histology products * Demonstrate Leica's line of histology and specimen prep instrumentation at customer locations * Prepare detailed quotations * Follow-up with potential customers at all levels - from end users to Lab Managers to Purchasing agents * Install systems and train customers on the proper use of Leica histology and specimen prep instrumentation * Work closely with Field Service Technicians and Leica Factory Representatives * Maintain customer database * Produce weekly call plans and monthly forecast * Participate in trade shows and seminars * Occasional travel to, and participation in, regional and national trade shows, training sessions, and product launches * Position reports to Director of Sales Job Requirements: * Minimum 1-3 years scientific/technical capital equipment or clinical sales experience and demonstrated success in achieving quota * Bachelor's degree in a Life Science discipline, or significant applications experience in this market * Understanding and experience with histology products and marketplace * Experience using Contact/Time Management software such as ACT! * Experience with Microsoft Office products * Extremely strong organizational skills * High energy individual driven to succeed in sales, capable of building relationships * Must possess a high level of integrity, entrepreneurial spirit, and be an excellent communicator and closer We offer a competitive, salary + commission, car allowance, and a comprehensive benefits package. Please send your resume to mcrump@mcbaininstruments.com. More company information is available at www.mcbaininstruments.com