[Histonet] pas

[Smith, Jeffery D. (HSC)]

Stock Light Green
    Light Green              1gm
    Distilled water       500 mL
    Glacial Acetic Acid   1mL
 Mix well and filter.  Stock solution stable for 3 to 4 months.
 
 
Working Light Green
     Stock light green        10mL
     Distilled Water            50mL
 
 

________________________________

From: BennettW <@t> pac.dfo-mpo.gc.ca [mailto:BennettW <@t> pac.dfo-mpo.gc.ca]
Sent: Tue 1/24/2006 3:55 PM
To: Smith, Jeffery D. (HSC)
Subject: RE: [Histonet] pas



Hi Diane,

What concentration of Light Green did you use?

Cheers
Bill Bennett
Histologist
Fisheries and Oceans Canada
Pacific Biological Station


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Smith,
Jeffery D. (HSC)
Sent: Tuesday, January 24, 2006 1:18 PM
To: Santana, Diane; Histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] pas


I run a routine PAS just changing the counterstain from hematoxylin to Light
Green.  The green masks most of the other components leaving bright pink
fungi on a pink background.

________________________________

From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Santana, Diane
Sent: Tue 1/24/2006 2:10 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] pas



Hi,
I am just curious on how others are doing a PAS for fungus? Thanks Diane
Santana

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