[Histonet] Anti-GRP-78

[Jackie M O'Connor]

Yep.  I use a Santa Cruz goat poly at 1:400 with the Vector Elite HRP kit. 
 I've tried it with both HIER and without - no difference - so no HIER is 
required.
JO'C





"Greg Dobbin" <dobbin <@t> upei.ca>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
01/20/2006 12:04 PM

 
        To:     histonet <@t> lists.utsouthwestern.edu
        cc:     djones <@t> avcn1.novell.upei.ca, (bcc: Jackie M O'Connor/LAKE/GPRD/ABBOTT)
        Subject:        [Histonet] Anti-GRP-78


Hello Everyone,

A colleague is about to stain (IHC) rat tissues (formalin fixed, paraffin 
embedded) for GRP-78. She has asked me to post her questions:

Does anyone out there have previous experience with this marker? 
And if so, do you have any tips, especially with regard to antigen 
retrieval methods (if necessary). (She doing a lit search as I write!)

Thanks so much.
Greg

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