[Histonet] tooth curl

[Rene J Buesa]

Trisha:
  Try adding 1 mL of Elmer's white glue in your regular 2-liter capacity round
  water bath which will be equivalent to a 0.05% solution, approx.
  You could even add some weight over the back of the slide with the section
  over a flat plastic surface. You should cover the plastic surface with paraffin oil
  (= mineral oil) to avoid the section to adhere to the plastic.
   As weight you could use some lead "sinkers", the ones used to sink the fishing lines.
  René J.

Trisha Emry <emry <@t> u.washington.edu> wrote:
  I just did serial sections through a decaled, paraffin embedded tooth.
I had trouble keeping sections stained with H & E on "plus" slides and they
often curled up at the edges and folded.

Any advice on how to deal with the ones cut and/or future teeth?

Thanks,
Trisha
U of Washington, Seattle


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
  




		
---------------------------------
Yahoo! Photos – Showcase holiday pictures in hardcover
 Photo Books. You design it and we’ll bind it!