[Histonet] antibodies to distinguish neurons and glia
    Caroline Bass 
    cbass <@t> bidmc.harvard.edu
       
    Thu Jan 12 20:16:25 CST 2006
    
    
  
Hi Guys,
I have  a protein being expressed in the brain and I would like to  
see if it is in neurons or glial cells.  Could someone recommend some  
good antibodies for this?  I think GFAP and NeuN are considered the  
standard.
I just need a reliable source for these.  If you have any suggestions  
as to dilutions and incubations times that would be great.
This is paraformaldehyde fixed mouse brain, 30 micron floating  
sections.  I am planning to do double fluorescent immunostaining.
Thanks,
Caroline Bass 
    
    
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