[Histonet] neurite visualization in cell culture

[John A. Kiernan]

Carri & Ebendal 1989

Coomassie brilliant blue R250 (CI 42660): 0.1g
Methanol: 25 ml
Acetic acid: 10 ml
Water to 100 ml

Stain for 20-30 minutes. 
For details see the original paper in Stain
Technology 64:50-52.
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John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan[AT]uwo.ca
   http://publish.uwo.ca/~jkiernan/
   http://instruct.uwo.ca/anatomy/530/index.htm
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Caroline Bass wrote:
> 
> Hello,
> 
> I am doing a neurite outgrowth assay in vitro and I need to add some
> contrast in order to visualize the neurites.  Is there a protocol for
> staining entire cells that will bring out this detail?  I just need
> to darken the entire cell and the neurite.  I have heard of some
> people using commassie blue for this purpose.  Does anyone have a
> protocol for this?
> 
> Thanks,
> 
> Caroline Bass