[Histonet] Plastic sectioning

[Sunhwa Lee]

Hi all

I am trying to cut 20-30um plastic sections of Zebrafish brain.  The
fish was perfused with AFA (alcohol-formalin-acetic acid) for more
than a month and treated with Technovit 7100 for plastic sections.
Then the sections were stained with Thionin.
(Thionin 15m à running tap water 5m à rinse in dH2O à Dry à DePex) But
it got shattering and lots of knife marks on it.  Any suggestion for
embedding and staining will be very appreciated.

Best,
Sunny