From chewy71874 <@t> aol.com Fri Dec 1 02:54:41 2006 From: chewy71874 <@t> aol.com (chewy71874@aol.com) Date: Fri Dec 1 02:54:56 2006 Subject: [Histonet] supervisor training Message-ID: <8C8E33D79B44CBC-5C0-3AD8@WEBMAIL-MA08.sysops.aol.com> Does anyone know of a good supervisor training course? I find myself in the position of supervising 3 other techs. Have had no formal training in supervising. Ellen ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From c.m.vanderloos <@t> amc.uva.nl Fri Dec 1 02:58:13 2006 From: c.m.vanderloos <@t> amc.uva.nl (C.M. van der Loos) Date: Fri Dec 1 02:58:35 2006 Subject: [Histonet] RE: Cell permeabilization and cell surface staining Message-ID: Dear Sonya, Sometimes theory and practice doesn't match with each other at all! That keeps us awake and makes our life both frustrating and interesting! I am afraid that the situations as you sketched are not that black-and-white in real life. For example there is a good change that the cells got a bit damaged, allowing the antibodies go in and out and having your internal proteins nicely stained. We once saw a difference between a cytospin and cells that were attached to glass (using the good old BioRad slides). The attachment procedure appeared to be more gentle to the cells than spinning them down with a great force, causing damaged cells at least at molecular level. Furthermore there might be a molecular reason why the internal antigen stained without Triton X100 treatment. Recently, Dick Dapson showed in JOH that formalin fixation followed by alcohol dehydration may even flip certain macromolecules inside-out. If this happens with your 'internal' protein you will get nice staining without the Triton X100 treatment. Why the Triton X100 treatment decreased your surface staining is difficult to understand. First thing that came up with me is that your antigen may shed from the membrane due to the Triton X100 treatment. Hope this helps a bit. Chris van der Loos, PhD Dept. of Pathology Academic Medical Center M2-230 Meibergdreef 9 NL-1105 AZ Amsterdam The Netherlands Date: Thu, 30 Nov 2006 10:27:57 -0000 From: "Martin S." Subject: [Histonet] Cell permeabilization and cell surface staining To: Hi all, I have been staining BMDC's to look at proteins which are present on the cell surface and in the cytoplasm. I fix the cells with 4% paraformaldehyde in PBS, 20min on ice and permeabilize with 0.2% Triton X100 in PBS for 20min at room temp. My two problems are: 1) When I dont permeabilize the cells I can still detect internal antigen with my primary antibody (cells are fixed 4% PFA, washed PBS and blocked with 2% BSA before incubation with antibody) 2) When I permeabilize the cells I get a decrease in the cell surface staining Any suggestions greatly appreciated. BW Sonya From sonya.martin <@t> soton.ac.uk Fri Dec 1 03:58:44 2006 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Fri Dec 1 04:00:01 2006 Subject: [Histonet] RE: Cell permeabilization and cell surface staining Message-ID: <71437982F5B13A4D9A5B2669BDB89EE4023E3499@ISS-CL-EX-V1.soton.ac.uk> Thanks for everyones help with this problem! Its good to know I'm not alone! Sonya From csn1x <@t> udcf.gla.ac.uk Fri Dec 1 05:59:47 2006 From: csn1x <@t> udcf.gla.ac.uk (Colin Nixon) Date: Fri Dec 1 05:59:56 2006 Subject: [Histonet] CD79 antibody Message-ID: <000d01c71540$32a346c0$d7e9d182@vet.gla.ac.uk> Does anyone know of a suitable CD79/B cell marker that works with Immunohistochemistry on formalin fixed paraffin embedded mice tissue? Colin From Dorothy.L.Webb <@t> HealthPartners.Com Fri Dec 1 07:31:35 2006 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Fri Dec 1 07:31:44 2006 Subject: [Histonet] Her2Neu Message-ID: <0E394B648E5284478A6CCB78E5AFDA27036407CF@hpes1.HealthPartners.int> Presently our pathologists only order Her2Neu off of the breast needle cores and not the excision or mastectomy. Would others please share there scope of practice regarding this area. Also, presently , we fix all breast tissue (other than needle cores or enblocs) in Davidson's and process with an alcoholic fixative from BBC. Will this cause problems with Her2Neu interpretation and/or other IHC concerns? Is there anyone else that uses an alcoholic formalin to process fatty tissue and has competent results with their IHC's? I would appreciate any and all feedback on this issue!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From rjbuesa <@t> yahoo.com Fri Dec 1 07:52:46 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Dec 1 07:52:53 2006 Subject: [Histonet] Her2Neu In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA27036407CF@hpes1.HealthPartners.int> Message-ID: <286993.51939.qm@web61224.mail.yahoo.com> We did Her2Neu on both needle and regular breast specimens. Theoretically any alcoholic fixative will not interfere with IHC; as a matter of fact they should interfere less than NBF. As interpretation goes I only foresee a problem because of the fact that probably the control slides you will use (if supplied by DAKO, for example) probably come from a culture not fixed in the same way as your specimen. In this respect you should ask for DAKO's imput. Ren? J. "Webb, Dorothy L" wrote: Presently our pathologists only order Her2Neu off of the breast needle cores and not the excision or mastectomy. Would others please share there scope of practice regarding this area. Also, presently , we fix all breast tissue (other than needle cores or enblocs) in Davidson's and process with an alcoholic fixative from BBC. Will this cause problems with Her2Neu interpretation and/or other IHC concerns? Is there anyone else that uses an alcoholic formalin to process fatty tissue and has competent results with their IHC's? I would appreciate any and all feedback on this issue!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Check out the all-new Yahoo! Mail beta - Fire up a more powerful email and get things done faster. From JWEEMS <@t> sjha.org Fri Dec 1 07:57:23 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Fri Dec 1 07:57:59 2006 Subject: [Histonet] Her2Neu Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEF463@sjhaexc02.sjha.org> And if your patient were to be included in a clinical trial, the Her2 testing would be required to have been carried out following the FDA approved protocol, which calls for fixation in 10% buffered formalin. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Friday, December 01, 2006 8:53 AM To: Webb, Dorothy L; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Her2Neu We did Her2Neu on both needle and regular breast specimens. Theoretically any alcoholic fixative will not interfere with IHC; as a matter of fact they should interfere less than NBF. As interpretation goes I only foresee a problem because of the fact that probably the control slides you will use (if supplied by DAKO, for example) probably come from a culture not fixed in the same way as your specimen. In this respect you should ask for DAKO's imput. Ren? J. "Webb, Dorothy L" wrote: Presently our pathologists only order Her2Neu off of the breast needle cores and not the excision or mastectomy. Would others please share there scope of practice regarding this area. Also, presently , we fix all breast tissue (other than needle cores or enblocs) in Davidson's and process with an alcoholic fixative from BBC. Will this cause problems with Her2Neu interpretation and/or other IHC concerns? Is there anyone else that uses an alcoholic formalin to process fatty tissue and has competent results with their IHC's? I would appreciate any and all feedback on this issue!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Check out the all-new Yahoo! Mail beta - Fire up a more powerful email and get things done faster. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From katherine-walters <@t> uiowa.edu Fri Dec 1 08:06:36 2006 From: katherine-walters <@t> uiowa.edu (Walters, Katherine S) Date: Fri Dec 1 08:06:44 2006 Subject: [Histonet] Info on lab microwaves Message-ID: I love my Biowave (Ted Pella). -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Drew Sally A. Sent: Thursday, November 30, 2006 2:54 PM To: Histonet Subject: [Histonet] Info on lab microwaves Our lab is using an older EMS Lab Microwave to do histochemical special stains and it needs to be replaced. What brand/model lab microwave ( not household) are others using for histochemical staining? Any positives or negatives to pass along? Sally Ann Drew, MT(ASCP) IHC/ISH Laboratory University of Wisconsin Hosp. & Clinics Madison, WI 53792 (608)265-6596 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From bakevictoria <@t> gmail.com Fri Dec 1 09:03:42 2006 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Fri Dec 1 09:03:59 2006 Subject: [Histonet] American Histo Labs Message-ID: <4f016b690612010703y5494636fx908880c91f8858b8@mail.gmail.com> Hi I'm trying to locate the phone number and contact person for this lab. I need to see about getting some cell blocks cut. Does anyone know about them? Thanks. Vikki From mtitford <@t> aol.com Fri Dec 1 12:33:53 2006 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Fri Dec 1 12:34:09 2006 Subject: [Histonet] American Histo Labs Message-ID: <8C8E38E634DE3A9-8C4-B88@MBLK-M13.sysops.aol.com> Vikki asks about American Histo Labs- That was the histology laboratory started by Lee Luna, a founding member of the National Society of Histotechnology. When I last heard, it was located in Gaithersburg, Maryland and Lee Luna's son managed it. If it is still in business, you should be able to locate it in the Gaithersburg white pages on the Internet. Mike Titford Pathology University of South Alabama Mobile AL 36617 ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From jwatson <@t> gnf.org Fri Dec 1 12:49:05 2006 From: jwatson <@t> gnf.org (James Watson) Date: Fri Dec 1 12:49:25 2006 Subject: [Histonet] American Histo Labs Message-ID: American Histolabs, Inc. Address: 7605-F Airpark Road: Gaithersburg, Maryland 20879: Phone: 3013301200: -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of mtitford@aol.com Sent: Friday, December 01, 2006 10:34 AM To: Histonet@pathology.swmed.edu Subject: [Histonet] American Histo Labs Vikki asks about American Histo Labs- That was the histology laboratory started by Lee Luna, a founding member of the National Society of Histotechnology. When I last heard, it was located in Gaithersburg, Maryland and Lee Luna's son managed it. If it is still in business, you should be able to locate it in the Gaithersburg white pages on the Internet. Mike Titford Pathology University of South Alabama Mobile AL 36617 ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From making <@t> ufl.edu Fri Dec 1 13:02:43 2006 From: making <@t> ufl.edu (MKing) Date: Fri Dec 1 13:00:56 2006 Subject: [Histonet] recruiter spam Message-ID: <45707C53.7090103@ufl.edu> anyone else annoyed that this listserve is being increasingly exploited by recruiters using it as a personal bulletin board? these posts today and yesterday with all the double and triple spacing and extra crap are simply spam. i move that histonet limit recruiters to posting links to their own sites where job hunters can find the information. let 'em host their own bandwidth. anyone second? mike king From Jeannette.Mitchell <@t> vtmednet.org Fri Dec 1 13:01:25 2006 From: Jeannette.Mitchell <@t> vtmednet.org (Mitchell, Jeannette M.) Date: Fri Dec 1 13:01:36 2006 Subject: [Histonet] microwave processing Message-ID: <8C7B2CE85A8CBA49B3FE05DE478412ED015B790C@FAHC14.fahc.fletcherallen.org> Our lab is looking into the possibility of Microwave processing. For those of you who have worked with Milestone RHS-1 microwave processor, what are the Pros and cons of the processor. Thanks for your assistance Jeannette Mitchell R&D Histotech FAHC Burlington,VT Confidentiality Notice: This message, and any attachments, may contain information that is confidential, privileged, and/or protected from disclosure under state and federal laws that deal with the privacy and security of medical information. If you received this message in error or through inappropriate means, please reply to this message to notify the Sender that the message was received by you in error, and then permanently delete this message from all storage media, without forwarding or retaining a copy. From TillRenee <@t> uams.edu Fri Dec 1 13:25:20 2006 From: TillRenee <@t> uams.edu (Till, Renee) Date: Fri Dec 1 13:26:08 2006 Subject: [Histonet] positioning tissues in OCT Message-ID: <11F927674DEBDC43B960809A7403C5D202A9E44E@MAILPED.ad.uams.edu> For our paraffin blocks we embed rat gi on end so when sectioned you get a little circle of tissue. Any suggestions on how to do this for cryosections? The fresh tissues have been too soft and flexible to stay standing up in the OCT until it freezes. Thanks. Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72202 Office (501)364-2785 Fax (501)364-3161 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From Jackie.O'Connor <@t> abbott.com Fri Dec 1 13:52:30 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Fri Dec 1 13:53:09 2006 Subject: [Histonet] positioning tissues in OCT In-Reply-To: <11F927674DEBDC43B960809A7403C5D202A9E44E@MAILPED.ad.uams.edu> Message-ID: Double embed them. Embed them flat - when that block hardens, trim and turn it 90 degrees to where you can re-embed it so you get the desired orientation. "Till, Renee" Sent by: histonet-bounces@lists.utsouthwestern.edu 12/01/2006 01:25 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] positioning tissues in OCT For our paraffin blocks we embed rat gi on end so when sectioned you get a little circle of tissue. Any suggestions on how to do this for cryosections? The fresh tissues have been too soft and flexible to stay standing up in the OCT until it freezes. Thanks. Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72202 Office (501)364-2785 Fax (501)364-3161 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hymclab <@t> hyhc.com Fri Dec 1 14:11:37 2006 From: hymclab <@t> hyhc.com (hymclab) Date: Fri Dec 1 14:07:53 2006 Subject: [Histonet] recruiter spam Message-ID: I truthfully like seeing what is going on our in the world. I am not looking for anyting right now but like to see what's available. When I'm busy I just hit the delete button and when I have time I do read them. For me it's no different than hitting delete on something that doesn't meet my realm of tesing. I don't feel that we are bombarded by these recruiter posts and don't feel we should ban them from our public listserv. Just my 2 cents worth. Dawn D. Schneider, HT(ASCP) Howard Young Medical Center Woodruff, WI 54568 (715)356-8174 schneiderd@hyhc.com -----Original Message----- From: MKing [mailto:making@ufl.edu] Sent: Friday, December 01, 2006 1:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] recruiter spam anyone else annoyed that this listserve is being increasingly exploited by recruiters using it as a personal bulletin board? these posts today and yesterday with all the double and triple spacing and extra crap are simply spam. i move that histonet limit recruiters to posting links to their own sites where job hunters can find the information. let 'em host their own bandwidth. anyone second? mike king _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipient(s) named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please notify the sender at the electronic mail address noted above and destroy all copies of this communication and any attachments. Thank you for your cooperation. From gcallis <@t> montana.edu Fri Dec 1 14:23:45 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Dec 1 14:23:57 2006 Subject: Intestine Re: [Histonet] positioning tissues in OCT In-Reply-To: <11F927674DEBDC43B960809A7403C5D202A9E44E@MAILPED.ad.uams.e du> References: <11F927674DEBDC43B960809A7403C5D202A9E44E@MAILPED.ad.uams.edu> Message-ID: <6.0.0.22.1.20061201130100.01b256d8@gemini.msu.montana.edu> For intestine, we rinse the lumen with PBS to remove fecal matter (it will be very crunchy when cryosectioned), then fill the lumen with pure OCT using a large gauge syringe needle, dulled. This distends the lumen a bit for support of inner structure (no bubbles, no flattened villi), OCT will ooze out a bit but in general does the job. You can either freeze longer gut segments then cut into workable sizes for double embedding or OR cut the OCT filled gut into shorter segements for embedding in Tissue Tek cryomold. Jackie O' Connor's suggestion was right on for double embedding, orientation on end, etc. Generally we mount block on metal disk then build up OCT around the block. Flat embedding/freezing/reorietation is our method for spinal cord too. Beware of which cryoembedding media you use to fill lumen - try different ones. We tried one media that was tested on human tissues only and injecting it into a lumen of mouse intestine failed to give good sections - the media didn't coat villi properly, and these were squished up and laddered. At 12:25 PM 12/1/2006, you wrote: >For our paraffin blocks we embed rat gi on end so when sectioned you get >a little circle of tissue. Any suggestions on how to do this for >cryosections? The fresh tissues have been too soft and flexible to stay >standing up in the OCT until it freezes. Thanks. > > > >Renee' Till, HT > >Research Assistant > >Arkansas Children's Nutrition Center > >1212 Marshall St./N2021 > >Little Rock, AR 72202 > >Office (501)364-2785 > >Fax (501)364-3161 > > > > >Confidentiality Notice: This e-mail message, including any attachments, is >for the sole use of the intended recipient(s) and may contain confidential >and privileged information. Any unauthorized review, use, disclosure or >distribution is prohibited. If you are not the intended recipient, please >contact the sender by reply e-mail and destroy all copies of the original >message. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From alaskagirl1950 <@t> yahoo.com Fri Dec 1 14:30:35 2006 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Fri Dec 1 14:30:48 2006 Subject: [Histonet] recruiter spam In-Reply-To: Message-ID: <975340.75997.qm@web52502.mail.yahoo.com> Sounds like my 2 cents worth also! I like knowing about job openings, and how little I make compared to others :-( ! But it might be good for there to be a web site that they could post on that we could than visit, might be a dream job out there for us all. Patrica --- hymclab wrote: > I truthfully like seeing what is going on our > in the world. I am not > looking for anyting right now but like to see > what's available. When I'm > busy I just hit the delete button and when I > have time I do read them. For > me it's no different than hitting delete on > something that doesn't meet my > realm of tesing. I don't feel that we are > bombarded by these recruiter posts > and don't feel we should ban them from our > public listserv. > Just my 2 cents worth. > > > Dawn D. Schneider, HT(ASCP) > Howard Young Medical Center > Woodruff, WI 54568 > (715)356-8174 > schneiderd@hyhc.com > > -----Original Message----- > From: MKing [mailto:making@ufl.edu] > Sent: Friday, December 01, 2006 1:03 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] recruiter spam > > anyone else annoyed that this listserve is > being increasingly exploited by > recruiters using it as a personal bulletin > board? these posts today and > yesterday with all the double and triple > spacing and extra crap are simply > spam. i move that histonet limit recruiters to > posting links to their own > sites where job hunters can find the > information. let 'em host their own > bandwidth. anyone second? > > mike king > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > CONFIDENTIALITY NOTICE: This e-mail > communication and any attachments may contain > confidential and privileged information for the > use of the designated recipient(s) named above. > If you are not the intended recipient, you are > hereby notified that you have received this > communication in error and that any review, > disclosure, dissemination, distribution or > copying of it or its contents is prohibited. If > you have received this communication in error, > please notify the sender at the electronic mail > address noted above and destroy all copies of > this communication and any attachments. Thank > you for your cooperation. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D ____________________________________________________________________________________ Yahoo! Music Unlimited Access over 1 million songs. http://music.yahoo.com/unlimited From asmith <@t> mail.barry.edu Fri Dec 1 14:37:18 2006 From: asmith <@t> mail.barry.edu (Smith, Allen) Date: Fri Dec 1 14:37:26 2006 Subject: [Histonet] recruiter spam In-Reply-To: <45707C53.7090103@ufl.edu> Message-ID: <7DBCCC1FBC77C94F99F920D0CA6400B61E4006@exchsrv01.barrynet.barry.edu> I like recruiter spam. I pass the best of it on to our histotechnology students. Allen A. Smith, Ph.D. Professor of Anatomy Barry University School of Graduate Medical Sciences Podiatric Medicine and Surgery Miami Shores, Florida 33161 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of MKing Sent: Friday, December 01, 2006 2:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] recruiter spam anyone else annoyed that this listserve is being increasingly exploited by recruiters using it as a personal bulletin board? these posts today and yesterday with all the double and triple spacing and extra crap are simply spam. i move that histonet limit recruiters to posting links to their own sites where job hunters can find the information. let 'em host their own bandwidth. anyone second? mike king _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses. Barry University - Miami Shores, FL (http://www.barry.edu) From Vfierke <@t> SNBLUSA.com Fri Dec 1 15:39:48 2006 From: Vfierke <@t> SNBLUSA.com (Vaughn Fierke) Date: Fri Dec 1 15:40:26 2006 Subject: [Histonet] unsubscribe Message-ID: <3CB1E2E5EC8E9C48A06C8E0967EB82B2A10E83@MAIL01.snblusa.com> unsubscribe Vaughn Fierke Confidentiality Notice: This letter, its contents and attachments are confidential and may contain privileged information. It is intended soley for the use of addressee(s) only. Any use, copying or disclosure of this communication or attachments to any other person is expressly prohibited without written permission of SNBL USA, Ltd. If you receive this message in error, please notify the sender at SNBL USA, Ltd immediately by return e-mail, telephone +1 425 407 0121, or fax +1 425 407 8601. We appreciate your cooperation. From dellav <@t> musc.edu Fri Dec 1 15:58:16 2006 From: dellav <@t> musc.edu (Vinnie Della Speranza) Date: Fri Dec 1 16:08:37 2006 Subject: [Histonet] control block for Pseudomonas aeruginosa Message-ID: Hi Liz, I haven't seen a reply to your question. I'm offering this information in the hope that it may offer a compromise solution to your need. American Type Culture Collection ( ATCC), www.atcc.com can provide you with a live culture of pseudomonas aeruginosa. keep in mind my last dealings with them was pre-9/11 so I don't know if this is as easy as it used to be. if you have access to a microbiology lab they should be able to grow this bug in a broth solution to prepare a sufficient volume that you could perfuse into fresh lung. my recollection is that this bug is not fastidious but I do recommend that you enlist the aid of those with BioSafety level II capability. I'm less certain of the difficulty in obtaining cystic fibrosis lung tissue and do not know of any commercial source for this tissue. good luck, Vinnie Vinnie Della Speranza Manager for Anatomic Pathology Services Medical University of South Carolina 165 Ashley Avenue Suite 309 Charleston, SC 29425 Ph: 843-792-6353 fax: 843-792-8974 >>> "Liz Chlipala" 11/29/06 03:54PM >>> Hello All I need to locate a block of human Cystic Fibrosis lung positive for P.aeruginosa. Does anyone know where I could get something like this. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ploykasek <@t> phenopath.com Fri Dec 1 16:22:24 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri Dec 1 16:22:40 2006 Subject: [Histonet] Her2Neu In-Reply-To: <0E394B648E5284478A6CCB78E5AFDA27036407CF@hpes1.HealthPartners.int> Message-ID: Hi Dorothy. I agree with some of the other posts that if you are using an FDA approved Her2 kit you should comply with the kit protocol which usually includes recommended fixation. We have noticed in our practice that alcoholic formalin increases the signal in our Her2 IHC. I would recommend that you have a subset of your IHC Her2 cases tested with Her2 FISH and see what your correlation is. This would give you an idea if your fixation is causing a false Her2 score. I would also like to recommend that techs educate themselves on the upcoming Her2 testing guidelines that ASCO & CAP will be implementing. The pathologists need to be aware of these too. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Presently our pathologists only order Her2Neu off of the breast needle > cores and not the excision or mastectomy. Would others please share > there scope of practice regarding this area. Also, presently , we fix > all breast tissue (other than needle cores or enblocs) in Davidson's and > process with an alcoholic fixative from BBC. Will this cause problems > with Her2Neu interpretation and/or other IHC concerns? Is there anyone > else that uses an alcoholic formalin to process fatty tissue and has > competent results with their IHC's? I would appreciate any and all > feedback on this issue!! > ________________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual responsible > for delivering the e-mail to the intended recipient, please be advised that > you have received this e-mail in error and that any use, dissemination, > forwarding, printing, or copying of this e-mail is strictly prohibited. > > If you have received this e-mail in error, please immediately notify the > HealthPartners Support Center by telephone at (952) 967-6600. You will be > reimbursed for reasonable costs incurred in notifying us. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From Eric <@t> ategra.com Fri Dec 1 19:50:26 2006 From: Eric <@t> ategra.com (Eric Dye (ext 223)) Date: Fri Dec 1 19:34:52 2006 Subject: [Histonet] Routine and non routine histology jobs hiring in your area?who do you know? Message-ID: Hi - Histonetters - Do you know of anyone I can speak to about the positions listed below? If you have IHC Experience: My hottest temporary Histology assignment is in Southern California for Histology Tech with IHC experience. This is a quick 4 week assignment. You will be home in time for the New year. If you're interested in hearing more details of this opening. Please call or shoot me an e-mail to discuss. Even, if you don't have any IHC experience: I have many other Permanent and Temporary openings for Histology Techs Nationwide that would fit your needs, SEE LIST BELOW for the updated list of both temp and perm Histology jobs, All openings are Dayshift Monday thru Friday unless indicated otherwise. All of these clients are currently looking to move quickly so if you are interested call me ASAP at 800-466-9919 ext 223 or cell - 407-756-5507. Histology Temporary Assignments (Updated Dec 01 2006) ------------------------------------------------------- 1. HOT ! - Southern California, Great Location - Must have IHC experience - Call Today- Needs To Be Filled By December 4th 2006 2. New Mexico - 8- 10 weeks -Histo Tech- Needed by November 20th 2006 -Still looking... 3. Eastern Massachusetts - Histo Tech minimum of 13 weeks (2 people) 4. Oklahoma - Histotech 8 weeks - FILLED Permanent Histology Jobs: (Updated Dec 01 2006) ------------------------------------------------------- 01. Southern Idaho- Histotech Coordinator - perm BrandNew 02. Central Florida - Histotech with some MOHS experience - perm - BrandNew HOT 03. Southern Alabama - Histotech - basic histology with opportunity to learn new and non-routine Histology -perm- BrandNew 04. Southeast South Dakota- Histotech - Must have ties to South Dakota- perm- BrandNew 05. Southwest Florida- Histotech Supervisor- perm - BrandNew (Need Florida License) 06. North Texas- Bench Histotech- Perm BrandNew 07. California (Bay Area) - Bench Histotech- perm -BrandNew 08. Texas (Dallas Area)- Bench Histotech- perm- BrandNew 09. Tennessee (Nashville Suburb) - Bench Histotech- perm- Hard Tissue (Bones) -BrandNew 10. Virginia (Northern) - Bench Histotech - perm- BrandNew 11. Southwest Texas- Bench Histotech- perm - BrandNew 12. HOT! Eastern, Massachusetts Seeking Histotechs of all experience levels, Great Pay, Location And Benefits- Call Today - 15% Pay Raise Guaranteed! 13. Washington, D.C. -Bench HistoTech- Diener- perm- BrandNew (AA Mortuary Science, Diener, 2-5 years Autopsy experience.) 14. NEW! Southeast Florida- Histotech - perm - (Need Florida License) 15. Ohio (Southern) - perm - Bench Histotech ( 2 openings) 16. Northern New Jersey - Histotech - perm 17. Eastern Mass - Histotech - one Senior Histotech, One not so Senior Histotech 18. Eastern Mass - Histotech - Histotech -perm 19. Massachusetts (North of Boston) - perm - Bench Histotech 20. Central Florida -perm- Histotech (Need Florida License) 21. Southeast Florida - Treasure Coast - perm - Histotech (Need Florida License) 22. Southeast Florida - perm - Histotech (Need Florida License) 23. South Florida - perm - Bench Histotech (Need Florida License) 24. Florida, West Coast - both temp & perm openings- Bench Histotech 25. New Hampshire perm openings - Bench Histotech 26. New York ( Syracuse area) - Bench Histotech- perm 27. Central Florida - Bench Histotech- perm -----------------------------------end perm jobs ---------------------------------------------------- -------------------------- end list of Histotech Opportunities ------------------------------------- If you are interested in any of the Histology jobs listed above - please call me at 800-466-9919 ext 223 or cell - 407-756-5507. Thank you, Eric Dye-Sr Allied Healthcare Recruiter 800-466-9919 ext 223 or Cell - 407-756-5507 P.S.: Feel free to pass along this email and my phone number to anyone who you think might be interested. P.S.S.: The clients are currently interviewing - and the job will close soon - so if you are interested, please call me today at 1-800-466-9919 ext 229 --------------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing 800 466 9919 ext 223 - voice 407 671 6075 - fax eric@ategra.com To Learn More About Ategra: http://www.ategra.com ---------------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. ---------------------------------------------------------------- ---------------------------------------------------------------- Note: this message is intended for: Histonetters at histonet@lists.utsouthwestern.edu ---------------------------------------------------------------- From cmiller <@t> physlab.com Sat Dec 2 08:27:06 2006 From: cmiller <@t> physlab.com (Cheri Miller) Date: Sat Dec 2 08:27:16 2006 Subject: [Histonet] recruiter spam In-Reply-To: <975340.75997.qm@web52502.mail.yahoo.com> Message-ID: <000001c7161d$f393a270$db01a8c0@plab.local> Ok, I will add my pennies to the pot. I like seeing what is available out there. Gives me a broad view of what HT opportunities I haven't seen before. I say just delete if your not interested. Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patricia Adams Sent: Friday, December 01, 2006 2:31 PM To: hymclab; HistoNet Subject: RE: [Histonet] recruiter spam Sounds like my 2 cents worth also! I like knowing about job openings, and how little I make compared to others :-( ! But it might be good for there to be a web site that they could post on that we could than visit, might be a dream job out there for us all. Patrica --- hymclab wrote: > I truthfully like seeing what is going on our > in the world. I am not > looking for anyting right now but like to see > what's available. When I'm > busy I just hit the delete button and when I > have time I do read them. For > me it's no different than hitting delete on > something that doesn't meet my > realm of tesing. I don't feel that we are > bombarded by these recruiter posts > and don't feel we should ban them from our > public listserv. > Just my 2 cents worth. > > > Dawn D. Schneider, HT(ASCP) > Howard Young Medical Center > Woodruff, WI 54568 > (715)356-8174 > schneiderd@hyhc.com > > -----Original Message----- > From: MKing [mailto:making@ufl.edu] > Sent: Friday, December 01, 2006 1:03 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] recruiter spam > > anyone else annoyed that this listserve is > being increasingly exploited by > recruiters using it as a personal bulletin > board? these posts today and > yesterday with all the double and triple > spacing and extra crap are simply > spam. i move that histonet limit recruiters to > posting links to their own > sites where job hunters can find the > information. let 'em host their own > bandwidth. anyone second? > > mike king > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > CONFIDENTIALITY NOTICE: This e-mail > communication and any attachments may contain > confidential and privileged information for the > use of the designated recipient(s) named above. > If you are not the intended recipient, you are > hereby notified that you have received this > communication in error and that any review, > disclosure, dissemination, distribution or > copying of it or its contents is prohibited. If > you have received this communication in error, > please notify the sender at the electronic mail > address noted above and destroy all copies of > this communication and any attachments. Thank > you for your cooperation. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D ____________________________________________________________________________ ________ Yahoo! Music Unlimited Access over 1 million songs. http://music.yahoo.com/unlimited _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LRaff <@t> lab.uropartners.com Sat Dec 2 09:07:19 2006 From: LRaff <@t> lab.uropartners.com (Lester Raff) Date: Sat Dec 2 09:07:25 2006 Subject: [Histonet] Cell blocks on urine specimens Message-ID: <5DA1CA5D0B98A84985B545A24423B822A584@UPLAB01.uplab.local> Hello Histonetters: Any information on doing cell blocks on urines would be appreciated. Specific questions include: 1) Can they be made on cytolyte fixed specimens? 2) What volume of specimen do they require? 3) Are they reviewed by the cytologist, or do they go straight to pathologist? 4) Are they reimbursable as an 88305 in addition to the cytology concentrated (Thin-Prep)88112 code? 5) Are they difficult to read? 6) Are they helpful? 7) How well do they correlate to thin-preps? Thanks, Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory ph: 708-486-0076 fax: 708-486-0080 From marjoh3 <@t> telus.net Sat Dec 2 13:34:38 2006 From: marjoh3 <@t> telus.net (Marilyn Johnson) Date: Sat Dec 2 13:34:52 2006 Subject: [Histonet] Thermostat For Tissue Tek II Embedding Center Message-ID: <001901c71648$e9991a90$6801a8c0@VALUED20606295> Hi Histonetters, I am looking for a thermostat for the upper compartment of a Tissue Tek II embedding center, Model 4603. Any replies would be greatly appreciated. Thank you in advance. Marilyn Johnson Edmonton, AB. Canada From dharclerode <@t> cytoritx.com Sat Dec 2 22:35:04 2006 From: dharclerode <@t> cytoritx.com (Donna Harclerode) Date: Sat Dec 2 22:33:18 2006 Subject: [Histonet] Tube cross sections in Frozens Message-ID: <3DE0F644E093DF4BAE80C254176696A5013E9E@mp-mailserver.macropore.com> Hi Renee' I multiple embed rat (and mouse) gi in Peel Away? embedding molds. I embed laying down, freeze and then cut the blocks on end. I put a bit of OCT in then arrange however many piecies I want in the block, then add more OCT until completely covered and recheck to see if any moved too much and then freeze. I line the tubes up to cut where the 2 dimples are in the molds (or you could mark the mold with a Sharpie) so I know which side to trim to get the correctt orientation. I can easily put 3-5 in a 22x22 or 22x30mm blocks. I end up with a very deep block (22mm) when I turn them on the end, but so far all my cryostats have not had probems doing this. I usually leave a piece of pancreas on the duodenum and put them in in order so I can tell which piece is which when they are in the blocks without a scope. Good luck Donna Harclerode HT(ASCP) HTL, QIHC Scientist/Immunohistochemistry Cytori Therapeutics 9020 Callan Rd San Diego, A 92021 heMessage: 5 Date: Fri, 1 Dec 2006 13:25:20 -0600 From: "Till, Renee" Subject: [Histonet] positioning tissues in OCT To: histonet@lists.utsouthwestern.edu Message-ID: <11F927674DEBDC43B960809A7403C5D202A9E44E@MAILPED.ad.uams.edu> Content-Type: text/plain; charset=us-ascii For our paraffin blocks we embed rat gi on end so when sectioned you get a little circle of tissue. Any suggestions on how to do this for cryosections? The fresh tissues have been too soft and flexible to stay standing up in the OCT until it freezes. Thanks. Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72202 Office (501)364-2785 Fax (501)364-3161 From billingconsultants <@t> yahoo.com Sun Dec 3 12:09:35 2006 From: billingconsultants <@t> yahoo.com (Billing Consultants, LLC) Date: Sun Dec 3 12:09:42 2006 Subject: [Histonet] Re: Thermostat for Tissue Tek II Embedding Center Message-ID: <530975.46652.qm@web54210.mail.yahoo.com> A good try would be Analytical Instruments. They are always very helpful. Their website is www.aibltd.com. Louri Roberts Billing Consultants, LLC www.billingconsultants.net histonet-request@lists.utsouthwestern.edu wrote: Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Thermostat For Tissue Tek II Embedding Center (Marilyn Johnson) 2. Tube cross sections in Frozens (Donna Harclerode) ---------------------------------------------------------------------- Message: 1 Date: Sat, 2 Dec 2006 12:34:38 -0700 From: "Marilyn Johnson" Subject: [Histonet] Thermostat For Tissue Tek II Embedding Center To: Message-ID: <001901c71648$e9991a90$6801a8c0@VALUED20606295> Content-Type: text/plain; charset="iso-8859-1" Hi Histonetters, I am looking for a thermostat for the upper compartment of a Tissue Tek II embedding center, Model 4603. Any replies would be greatly appreciated. Thank you in advance. Marilyn Johnson Edmonton, AB. Canada ------------------------------ Message: 2 Date: Sat, 2 Dec 2006 20:35:04 -0800 From: "Donna Harclerode" Subject: [Histonet] Tube cross sections in Frozens To: Cc: TillRenee@uams.edu Message-ID: <3DE0F644E093DF4BAE80C254176696A5013E9E@mp-mailserver.macropore.com> Content-Type: text/plain; charset="iso-8859-1" Hi Renee' I multiple embed rat (and mouse) gi in Peel Away? embedding molds. I embed laying down, freeze and then cut the blocks on end. I put a bit of OCT in then arrange however many piecies I want in the block, then add more OCT until completely covered and recheck to see if any moved too much and then freeze. I line the tubes up to cut where the 2 dimples are in the molds (or you could mark the mold with a Sharpie) so I know which side to trim to get the correctt orientation. I can easily put 3-5 in a 22x22 or 22x30mm blocks. I end up with a very deep block (22mm) when I turn them on the end, but so far all my cryostats have not had probems doing this. I usually leave a piece of pancreas on the duodenum and put them in in order so I can tell which piece is which when they are in the blocks without a scope. Good luck Donna Harclerode HT(ASCP) HTL, QIHC Scientist/Immunohistochemistry Cytori Therapeutics 9020 Callan Rd San Diego, A 92021 heMessage: 5 Date: Fri, 1 Dec 2006 13:25:20 -0600 From: "Till, Renee" Subject: [Histonet] positioning tissues in OCT To: histonet@lists.utsouthwestern.edu Message-ID: <11F927674DEBDC43B960809A7403C5D202A9E44E@MAILPED.ad.uams.edu> Content-Type: text/plain; charset=us-ascii For our paraffin blocks we embed rat gi on end so when sectioned you get a little circle of tissue. Any suggestions on how to do this for cryosections? The fresh tissues have been too soft and flexible to stay standing up in the OCT until it freezes. Thanks. Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72202 Office (501)364-2785 Fax (501)364-3161 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 37, Issue 3 *************************************** --------------------------------- Check out the all-new Yahoo! Mail beta - Fire up a more powerful email and get things done faster. From jpastor1 <@t> nycap.rr.com Sun Dec 3 15:08:06 2006 From: jpastor1 <@t> nycap.rr.com (joseph pastore) Date: Sun Dec 3 15:00:41 2006 Subject: [Histonet] RE: Recruiter spam Message-ID: <001d01c7171f$213bca20$7864a8c0@JGPONLINE> I for one would like to see the job openings. You never know when something interesting might come up. From jpastor1 <@t> nycap.rr.com Sun Dec 3 15:31:46 2006 From: jpastor1 <@t> nycap.rr.com (joseph pastore) Date: Sun Dec 3 15:24:18 2006 Subject: [Histonet] Non Formalin fixatives Message-ID: <002401c71722$6f62eeb0$7864a8c0@JGPONLINE> Hi all, I am trying to reduce the volume of formalin and solvent waste in my Lab. Has anyone had experience and comments on non formalin fixatives? Also, I have been able to use Aqua DePar from Biocare to deparaffinize my sections for H&E, special stains and immunohistochemistry with great results but would also like to try an H&E without alcohol differentiation and dehydration. Does anyone know of a H&E procedure that does not use alcohol after eosin? From scoop <@t> mail.nih.gov Sun Dec 3 20:30:46 2006 From: scoop <@t> mail.nih.gov (scoop@mail.nih.gov) Date: Sun Dec 3 20:31:00 2006 Subject: [Histonet] supermount (from BioGenex) for immunofluorescence? Message-ID: Dear All, Has anyone used Supermount from Biogenex for mounting tissue sections stained by immunofluorescence? I've used it for mounting non-fluorescent tissue section and it was fine but I would like to try it for IF. Any opinions/advice would be appreciated. Thanks, Sharon From Kemlo.Rogerson <@t> waht.swest.nhs.uk Mon Dec 4 02:19:01 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Dec 4 02:17:57 2006 Subject: [Histonet] Non Formalin fixatives Message-ID: <86ADE4EB583CE64799A9924684A0FBBF01246F01@wahtntex2.waht.swest.nhs.uk> You want it with mercury, lead, iron, dichromate or gluteraldehyde? My point is that formalin is probably to most innocuous. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 A belief is purely an individual matter, and you cannot and must not organize it. --J. Krishnamurti This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From M.Walker <@t> hrsu.mrc.ac.uk Mon Dec 4 03:57:53 2006 From: M.Walker <@t> hrsu.mrc.ac.uk (Marion Walker) Date: Mon Dec 4 03:58:10 2006 Subject: [Histonet] Stem cell factor (Steel Factor) Message-ID: <5EF1494538AC184E86844A6886D984300118C0EE@mailserv.hrsu.mrc.ac.uk> Hi All, Has anyone successfully stained for stem cell factor in formalin fixed, paraffin embedded rat tissue? If so, would you mind sharing any advice and/or protocols? Any help would be greatly appreciated. Marion Walker Human Reproductive Sciences Unit Edinburgh Scotland UK From ryakay <@t> shands.ufl.edu Mon Dec 4 06:54:28 2006 From: ryakay <@t> shands.ufl.edu (Kaye Ryan) Date: Mon Dec 4 06:55:01 2006 Subject: [Histonet] microwave processing Message-ID: Hi Jeannette, We have used the RHS-1 and 2 for over three years now and love it. We are currently only processing our biopsies but we have just installed the PATHOS which will do all tissue types. The pro's for utilizing the microwave technology are of course the turn-around-times. We are able to process small biopsies in just under an hour and have them ready for embedding. The tissues cut so much better and the stain quality is wonderful. We used to have a lot of problems with chatter in our gastric and colon biopsies and this is virtually done away with when using the microwave processing. I am one of those "die hard" histologists that has to have everything proven to me and I am totally sold on the microwave, especially the Milestone model. You only use three reagents so there is a good savings in reagents cost and if you do not recycle, the hauling of hazardous waste. The only real "con" I can see is that you have to be there to change the solutions when the timer goes off. This is done away with the PATHOS as it is fully automated. We are very excited about all the possibilities with this technology. Hope this helps answer some questions, Sincerely, Kaye Ryan Kaye Ryan Histology Manager/Educational Coordinator Rocky Point Laboratories 265-0111, 72093 >>> "Mitchell, Jeannette M." 12/1/2006 2:01 PM >>> Our lab is looking into the possibility of Microwave processing. For those of you who have worked with Milestone RHS-1 microwave processor, what are the Pros and cons of the processor. Thanks for your assistance Jeannette Mitchell R&D Histotech FAHC Burlington,VT Confidentiality Notice: This message, and any attachments, may contain information that is confidential, privileged, and/or protected from disclosure under state and federal laws that deal with the privacy and security of medical information. If you received this message in error or through inappropriate means, please reply to this message to notify the Sender that the message was received by you in error, and then permanently delete this message from all storage media, without forwarding or retaining a copy. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Mon Dec 4 09:28:00 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Dec 4 09:28:08 2006 Subject: [Histonet] Non Formalin fixatives In-Reply-To: <002401c71722$6f62eeb0$7864a8c0@JGPONLINE> References: <002401c71722$6f62eeb0$7864a8c0@JGPONLINE> Message-ID: <6.0.0.22.1.20061204081930.01b51b60@gemini.msu.montana.edu> Why not consider purchasing recyclers for solvents and for formalin. People or labs using these like them and have reduced both the cost of collection, packaging and shipping wastes considerably. You still need to use absolute alcohols since recycling only produces alcohols to 95%. Propar and Clearite 3 can be recyled also. Check out the archives on recycling, there has been discussion on this many time. At 02:31 PM 12/3/2006, you wrote: >Hi all, >I am trying to reduce the volume of formalin and solvent waste in my Lab. >Has anyone had experience and comments on non formalin fixatives? Also, I >have been able to use Aqua DePar from Biocare to deparaffinize my sections >for H&E, special stains and immunohistochemistry with great results but >would also like to try an H&E without alcohol differentiation and >dehydration. Does anyone know of a H&E procedure that does not use alcohol >after eosin? >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From pmarcum <@t> vet.upenn.edu Mon Dec 4 09:49:35 2006 From: pmarcum <@t> vet.upenn.edu (pmarcum@vet.upenn.edu) Date: Mon Dec 4 09:49:44 2006 Subject: [Histonet] Non Formalin fixatives In-Reply-To: <6.0.0.22.1.20061204081930.01b51b60@gemini.msu.montana.edu> References: <002401c71722$6f62eeb0$7864a8c0@JGPONLINE> <6.0.0.22.1.20061204081930.01b51b60@gemini.msu.montana.edu> Message-ID: <1165247375.4574438fe002d@imp.vet.upenn.edu> Gayle is right it can reduce your disposal cost and will pay for itself in a short period of time. Also with formalin subs you have to be sure none of your antibodies are linked to an FDA protocol that requires formalin as the fixative for a specific drug to be given based on the test results. You would need to re-titer or check every antibody against your formalin results to be sure they will not need adjustments. I am hoping to begin recylcling next year dispite the fact we get our alcohols through the university for a very good price. The cost of xylene and xylene substiutes is still high. Pam Marcum Quoting Gayle Callis : > Why not consider purchasing recyclers for solvents and for formalin. People > or labs using these like them and have reduced both the cost of collection, > packaging and shipping wastes considerably. You still need to use absolute > alcohols since recycling only produces alcohols to 95%. Propar and > Clearite 3 can be recyled also. Check out the archives on recycling, > there has been discussion on this many time. > > At 02:31 PM 12/3/2006, you wrote: > >Hi all, > >I am trying to reduce the volume of formalin and solvent waste in my Lab. > >Has anyone had experience and comments on non formalin fixatives? Also, I > >have been able to use Aqua DePar from Biocare to deparaffinize my sections > >for H&E, special stains and immunohistochemistry with great results but > >would also like to try an H&E without alcohol differentiation and > >dehydration. Does anyone know of a H&E procedure that does not use alcohol > >after eosin? > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 > 406 994-4303 (FAX) > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From awright <@t> mercedesmedical.com Mon Dec 4 10:09:52 2006 From: awright <@t> mercedesmedical.com (Andy Wright) Date: Mon Dec 4 10:10:02 2006 Subject: [Histonet] December Web Specials and Holiday Gift Giveaway Message-ID: <1101473555931.1011370901330.8700.7.21105BE@scheduler> Forward email http://ui.constantcontact.com/sa/fwtf.jsp?m=1011370901330&ea=histonet%40pathology.swmed.edu&a=1101473555931 This email was sent to histonet@pathology.swmed.edu, by awright@mercedesmedical.com Update Profile/Email Address http://ui.constantcontact.com/d.jsp?p=oo&m=1011370901330&ea=histonet%40pathology.swmed.edu&se=8700&t=1101473555931&lang=en&reason=F Instant removal with SafeUnsubscribe(TM) http://ui.constantcontact.com/d.jsp?p=un&m=1011370901330&ea=histonet%40pathology.swmed.edu&se=8700&t=1101473555931&lang=en&reason=F Privacy Policy: http://ui.constantcontact.com/roving/CCPrivacyPolicy.jsp Powered by Constant Contact(R) www.constantcontact.com Mercedes Medical | 7590 Commerce Court | Sarasota | FL | 34243 From PCrean <@t> holyredeemer.com Mon Dec 4 10:41:31 2006 From: PCrean <@t> holyredeemer.com (Pat Crean) Date: Mon Dec 4 10:43:31 2006 Subject: [Histonet] cpt code question Message-ID: Hi everyone, Could someone help me? I am looking for what the cpt code would be for a PPV/retinal peel. Not sure what I should what I should be charging. Thanks for any help I can get. Pat Pat Crean Histology Technician Holy Redeemer Hosp. and Med. Ctr. 1948 Huntingdon Pike Meadowbrook, PA 19046 ph (215) 938-3676 CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. From anh2006 <@t> med.cornell.edu Mon Dec 4 10:47:45 2006 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Mon Dec 4 10:48:22 2006 Subject: [Histonet] F4/80 quick question Message-ID: Does F4/80 from Serotec work on frozen sections of mouse tissue? -- From kamaid <@t> iibce.edu.uy Mon Dec 4 11:36:42 2006 From: kamaid <@t> iibce.edu.uy (andres) Date: Mon Dec 4 11:37:01 2006 Subject: [Histonet] Comments on BRDU detection In-Reply-To: <20061014180728.F1E3555F7C@iibce.edu.uy> References: <20061014180728.F1E3555F7C@iibce.edu.uy> Message-ID: <6.2.1.2.0.20061204135211.03559a18@localhost> Dear "Histonetters", I've just gone over all the archived messages in the list about "BRDU" and I have to say I've learnt a lot about it (I confirm the utility of the list, and congratulate you all for make it work) . However, I still have a question and a comment I wanted to post to you. We are doing fluorescent BrdU detection on 20 um crystotat sections of chick embryos (parafolmadehyde fixed and sucrose cryoprotected). First, I'd like to share my results with you as it might may be of any help, second ask if someone has seen the same kind of thing, and finally, try to find a way to solve the following: When we treat the sections with 2N HCl for 30 min RT, appart from the poorly conserved nuclear morphology that seem to be common for many colleagues (although we still get DAPI staining reasonably conserved), we ONLY get BrdU staining in the 5-15 um surface of the section (as evaluated by confocal z-scanning). This is specific for BrdU detection, since double immunofluorescence with other antibodies penetrate the whole 20 um of the specimen. These low penetration results are very consistant, and it's not a problem of particular solutions or tissue sections. Increasing the time and temperature of the acid treatment (e.g. 2N HCl for 2 hs, RT or 37?C) seems to improve the "penetration" of the staining, although DAPI or TOPRO staining is lost. More interestingly, the improvement is only seen in the mesenchymal tissues, but NOT in more dense tissues (particularly, the neuroepithelium of the brain or spinal cord). So in those tissues we still get ONLY about 5 um-deep staining. I suspect this is related to insufficient DNA denaturation in those tissues, but as I said this is already quite a strong treatment. Most of the people use the HCl treatment, even with shorter times, but I haven't seen any report of poor penetration of the BrdU stain, so obviously I wonder if there's anything we're doing wrong. However, we also think that for many people this issue may not be a problem, and may not even realized about it, specially if you use chromogenic reactions like DAB. UNfortunately, for some purposes it may actually be very important, so I'd like to know if any of you have also checked and/or seen this effect, specially those of you working with tight epithelia or nervous system. Also, if there's any alternative to overcome the acid treatment (we've tried citric acid Antigen retrieval but didn't get any signal at all, and formamide treatment doesn't improve it either). I apologize for the long message, and I hope to get your inputs or comments on this. Once again thank you, A Andr?s Kamaid Developmental Biology Group Universitat Pompeu Fabra Barcelona -Spain From Frederick.Fifield <@t> sunhealth.org Mon Dec 4 11:51:28 2006 From: Frederick.Fifield <@t> sunhealth.org (Frederick.Fifield@sunhealth.org) Date: Mon Dec 4 11:51:45 2006 Subject: [Histonet] Decolorizing Giemsa Stained Bone Marrow Smears Message-ID: Is there a safe way to decolorize a giemsa stained bone marrow? Thank you for your help. Fred Fifield BS, HT (ASCP) Pathology Section Manager Sun Health - Boswell Memorial Hospital (623) 876-5338 ******************************************************************************* The information contained in this transmission may be legally privileged and/or confidential information. Any dissemination, distribution or copying of this transmission by anyone other than the intended recipient is strictly prohibited. If you receive this in error, please inform the sender immediately and remove any record of this message. ******************************************************************************* For more information about Sun Health, visit us at: www.sunhealth.org From Annette_hall <@t> pa-ucl.com Mon Dec 4 12:20:55 2006 From: Annette_hall <@t> pa-ucl.com (Annette Hall) Date: Mon Dec 4 12:21:08 2006 Subject: [Histonet] Decreased Staining on Controls Message-ID: <9FC023A4AB52BB4D87DC6456081A822C012B5902@mercury.pa-ucl.com> Hi, We are having problems with are CD15 and CD30 antibodies using the DakoCytomation Artisan. The controls seem lighter than when we first started with this instrument 4 years ago. Our instruction has been to use "fresh" controls. We cut controls and store them at 4 deg C for a few months. In your experience, what do you consider fresh? How long do you keep cut slides controls before you toss them? Thanks, Annette J Hall, MT Micro/Cyto/Histo Supervisor United Clinical Labs 205 Bluff Street Dubuque, IA 52001 Phone: 563.556.2010 x131 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. From gcallis <@t> montana.edu Mon Dec 4 12:24:08 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Dec 4 12:24:27 2006 Subject: [Histonet] F4/80 quick question In-Reply-To: References: Message-ID: <6.0.0.22.1.20061204111335.01b3a648@gemini.msu.montana.edu> Yes, and it works very well. It is in our panel of murine antibodies. Our frozen sections are air dried overnight then fixed in acetone 75%/ absolute ethanol 25% 5 min at RT, then directly into buffer after fixation without any further air drying. We did a dilution panel for our laboratory conditions, and used a goat antiRat F(ab')2 secondary, Strepavidin-HRP/AEC with all the appropriate blocking step. We use this antibody for both immunohistochemistry and immunofluorescence staining. At 09:47 AM 12/4/2006, you wrote: >Does F4/80 from Serotec work on frozen sections of mouse tissue? >-- Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From petepath <@t> yahoo.com Mon Dec 4 12:31:57 2006 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Mon Dec 4 12:32:04 2006 Subject: [Histonet] Histonet] Tube cross sections in Frozens Message-ID: <808948.35059.qm@web30404.mail.mud.yahoo.com> Dear Renee, This can be accomplished easily using frozen block cryoenmbedding. This is a two step embedding process. First the tissue is frozen in a block of embedding medium. This now firm easy to cut frozen block is then sectioned on edge. The resulting slices are then embedded on edge either face down in a well ( mold) or just by lying it face up on a chuck. You can do the first freeze between any two pieces of freezing temp metal. This process is detailed at: http://pathologyinnovations.com/frozen_block_embedding.htm Stephen Stephen Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com From GDawson <@t> dynacaremilwaukee.com Mon Dec 4 12:22:21 2006 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Mon Dec 4 12:51:30 2006 Subject: [Histonet] Non Formalin fixatives Message-ID: All, Non formalin fixatives are, in my opinion, a poor choice for almost any lab. Unless your lab is completely self sufficient and would never need to send blocks out for testing or consult, you will run into many possible problems when you need testing that you do not perform yourself. Since the vast majority of labs use formalin fixation, another fixation method will require not only different protocols for testing like IHC, but also would require a bank of known positive controls processed with the same non-formalin fixative. In other words, if you were to need an IHC stain from an outside lab, it is highly unlikely that they would have all the appropriate controls needed processed with the same non-formalin fixative that you are using. Some FDA approved kits mandate formalin fixation for the kit to be valid. I have a client that used a fixative that "made their H&E's and Trichromes absolutely beautiful" but when they needed my FDA approved HercepTest run on them, it was not possible specifically because of the fixation. This persuaded them to switch over to formalin. As histology professionals, it would benefit all of us if we used the same fixative. Formalin, with all its faults, is our best bet for a "universal fixative" since it is in the vast majority of histo labs. My opinion, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI From tkngflght <@t> yahoo.com Mon Dec 4 13:32:47 2006 From: tkngflght <@t> yahoo.com (Cheryl) Date: Mon Dec 4 13:32:57 2006 Subject: [Histonet] seeking source--Microtome block chuck alignment tool In-Reply-To: <5EF1494538AC184E86844A6886D984300118C0EE@mailserv.hrsu.mrc.ac.uk> Message-ID: <968478.69568.qm@web50903.mail.yahoo.com> Hi Everyone I am looking for the vendor source for the gidget that aligns a block chuck so all the microtomes will match. It's a universal sledge with an aluminum or steel 'block' that fits into the holder to square it up in all three dimensions. Does anyone know where to find them and about how much they are these days? Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one Tech at a time. 281.883.7704 c 281.852.9457 o admin@fullstaff.org Sign up for the FREE monthly newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. From pmarcum <@t> vet.upenn.edu Mon Dec 4 13:40:46 2006 From: pmarcum <@t> vet.upenn.edu (pmarcum@vet.upenn.edu) Date: Mon Dec 4 13:40:57 2006 Subject: [Histonet] REGION II MEETING SAVE THESE DATES Message-ID: <1165261246.457479bebfb6d@imp.vet.upenn.edu> Good Afternoon, We are back in Region II with a message to save these dates September 7th and 8th, 2007 for our meeting. We have moved our meeting from November to avoid a close conflict with the NSH Meeting in Denver, CO. We will be sending the program in the forst quarter of '07. If you have questions let me know. The meeting will be in north Delaware so plan to join us. Pamela Marcum Education Coordinator From pmarcum <@t> vet.upenn.edu Mon Dec 4 13:45:42 2006 From: pmarcum <@t> vet.upenn.edu (pmarcum@vet.upenn.edu) Date: Mon Dec 4 13:45:50 2006 Subject: [Histonet] Non Formalin fixatives In-Reply-To: References: Message-ID: <1165261542.45747ae69804b@imp.vet.upenn.edu> Very good answer and it is what we face now when new products are introduced. We all want to try them before we truly understand all the possible consequences. As much as we may,at times want to get rid of formalin it is the empirical fixative that has been used and tested for almost all of the stains and IHCs we currently do as a routine in both clinical and research. Pam marcum Quoting "Dawson, Glen" : > All, > > Non formalin fixatives are, in my opinion, a poor choice for almost any lab. > > Unless your lab is completely self sufficient and would never need to send > blocks out for testing or consult, you will run into many possible problems > when you need testing that you do not perform yourself. Since the vast > majority of labs use formalin fixation, another fixation method will require > not only different protocols for testing like IHC, but also would require a > bank of known positive controls processed with the same non-formalin > fixative. > > In other words, if you were to need an IHC stain from an outside lab, it is > highly unlikely that they would have all the appropriate controls needed > processed with the same non-formalin fixative that you are using. > > Some FDA approved kits mandate formalin fixation for the kit to be valid. I > have a client that used a fixative that "made their H&E's and Trichromes > absolutely beautiful" but when they needed my FDA approved HercepTest run on > them, it was not possible specifically because of the fixation. This > persuaded them to switch over to formalin. > > As histology professionals, it would benefit all of us if we used the same > fixative. Formalin, with all its faults, is our best bet for a "universal > fixative" since it is in the vast majority of histo labs. > > My opinion, > > Glen Dawson BS, HT & QIHC (ASCP) > IHC Manager > Milwaukee, WI > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Mon Dec 4 14:51:43 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Dec 4 14:51:53 2006 Subject: [Histonet] Decolorizing Giemsa Stained Bone Marrow Smears In-Reply-To: Message-ID: <302885.27557.qm@web61214.mail.yahoo.com> I always did that with the acid alcohol used for H&E differentiation. Never had a problem. After the procedure neutralize the smear with lithium carbonate solution (the same used for blueing H&E).. Ren? J. Frederick.Fifield@sunhealth.org wrote: Is there a safe way to decolorize a giemsa stained bone marrow? Thank you for your help. Fred Fifield BS, HT (ASCP) Pathology Section Manager Sun Health - Boswell Memorial Hospital (623) 876-5338 ******************************************************************************* The information contained in this transmission may be legally privileged and/or confidential information. Any dissemination, distribution or copying of this transmission by anyone other than the intended recipient is strictly prohibited. If you receive this in error, please inform the sender immediately and remove any record of this message. ******************************************************************************* For more information about Sun Health, visit us at: www.sunhealth.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Have a burning question? Go to Yahoo! Answers and get answers from real people who know. From rjbuesa <@t> yahoo.com Mon Dec 4 14:57:57 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Dec 4 14:58:04 2006 Subject: [Histonet] Decreased Staining on Controls In-Reply-To: <9FC023A4AB52BB4D87DC6456081A822C012B5902@mercury.pa-ucl.com> Message-ID: <20061204205757.55777.qmail@web61221.mail.yahoo.com> Annette: Control sections oxidize and the antigenic activity decreases (I am sending you under separate cover a paper I wrote on the subject). I always tried to have enough controls for 1 week's worth of IHC procedures. After 2-3 weeks the weakening started. Even at low temperature this will occur (although more slowly because of the reduction in the oxidation speed because of a lower temperature). A student I tutored for a BMS degree (in the UK) wrote his thesis on this subject. The best way she found in protecting the sections was storing them in a nitrogen atmosphere, but this, as a normal practice, will be somewhat difficult. The safest way is to have just what you need. Several months is too much. Hope this (and the article) will help you! Ren? J. Annette Hall wrote: Hi, We are having problems with are CD15 and CD30 antibodies using the DakoCytomation Artisan. The controls seem lighter than when we first started with this instrument 4 years ago. Our instruction has been to use "fresh" controls. We cut controls and store them at 4 deg C for a few months. In your experience, what do you consider fresh? How long do you keep cut slides controls before you toss them? Thanks, Annette J Hall, MT Micro/Cyto/Histo Supervisor United Clinical Labs 205 Bluff Street Dubuque, IA 52001 Phone: 563.556.2010 x131 NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Cheap Talk? Check out Yahoo! Messenger's low PC-to-Phone call rates. From gentras <@t> vetmed.auburn.edu Mon Dec 4 15:07:33 2006 From: gentras <@t> vetmed.auburn.edu (Atoska Gentry) Date: Mon Dec 4 15:07:39 2006 Subject: [Histonet] analytical balance Message-ID: <45748E15.5060308@vetmed.auburn.edu> hello, does anyone have contact info on a source that provides replacement parts for the Mettler Toledo Analytical Balance Model AB204? I am in need of a side drafting shield. One of ours slipped off track & broke today. Your prompt replies will be greatly appreciated. Atoska -- Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu From Cathy.Crumpton <@t> tuality.org Mon Dec 4 18:03:49 2006 From: Cathy.Crumpton <@t> tuality.org (Cathy.Crumpton@tuality.org) Date: Mon Dec 4 18:04:02 2006 Subject: [Histonet] Cathy Crumpton is out of the office. Message-ID: I will be out of the office starting Mon 12/04/2006 and will not return until Mon 04/02/2007. If you have any histology concerns please route them to Connie Basinski during my absence. From bjdewe <@t> aol.com Mon Dec 4 18:16:00 2006 From: bjdewe <@t> aol.com (bjdewe@aol.com) Date: Mon Dec 4 18:16:10 2006 Subject: [Histonet] Animal Control Slides Message-ID: <8C8E619AD855A32-D48-9CF2@FWM-D32.sysops.aol.com> Hi List, I am once again listing the website for the Animal Control slides. If there is any interest the site has been updated and is much easier to navigate ;-) http://www.innvx.com/ Cheers, Lorie Live as if you were to die tomorrow. Learn as if you were to live forever. - Gandhi - ******************************************************************* Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s)and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ******************************************************************* ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From sonya.martin <@t> soton.ac.uk Tue Dec 5 04:09:07 2006 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Tue Dec 5 04:09:57 2006 Subject: [Histonet] RE:F4/80 quick question Message-ID: <71437982F5B13A4D9A5B2669BDB89EE4023E34A3@ISS-CL-EX-V1.soton.ac.uk> I have used biotinylated F4/80 from serotec on acetone fixed frozen sections of mouse spleen and it works really nicely! Sonya From kmerriam2003 <@t> yahoo.com Tue Dec 5 07:06:36 2006 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Tue Dec 5 07:06:47 2006 Subject: [Histonet] disposable cryostat object discs Message-ID: <20061205130636.61243.qmail@web50307.mail.yahoo.com> Hello, Does anyone know of a vendor that sells disposable (plastic) cryostat object discs? Kim Kim Merriam Cambridge, MA ____________________________________________________________________________________ Any questions? Get answers on any topic at www.Answers.yahoo.com. Try it now. From rjbuesa <@t> yahoo.com Tue Dec 5 09:19:11 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Dec 5 09:26:01 2006 Subject: [Histonet] analytical balance In-Reply-To: <45748E15.5060308@vetmed.auburn.edu> Message-ID: <20061205151911.86338.qmail@web61217.mail.yahoo.com> Once that happened to me also. I replaced it with a thin Perplex sheet (cut to fit). Try this solution! Ren? J. Atoska Gentry wrote: hello, does anyone have contact info on a source that provides replacement parts for the Mettler Toledo Analytical Balance Model AB204? I am in need of a side drafting shield. One of ours slipped off track & broke today. Your prompt replies will be greatly appreciated. Atoska -- Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Everyone is raving about the all-new Yahoo! Mail beta. From kewleys <@t> health.missouri.edu Tue Dec 5 09:28:24 2006 From: kewleys <@t> health.missouri.edu (Kewley, Sharyl F.) Date: Tue Dec 5 09:28:35 2006 Subject: [Histonet] Unsubscribe!! Message-ID: <93D0418A67D27C47BD90CF3A0DCC39F524A291@UM-XMAIL04.um.umsystem.edu> Please unsubscribe me (kewleys@health.missouri.edu. I will be retiring on Dec.22, 2006. And to all of the people I know out htere in "histoland", it's been a great 34 years and I still love the field. However, I will be moving on to the next phase of my life, yipes! how did I get this old. Keep those microtomes cutting. Godspeed, Sharyl Kewley Columbia Regional Hospital 404 Keene St. Columbia, MO 65201 From sonya.martin <@t> soton.ac.uk Tue Dec 5 09:17:30 2006 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Tue Dec 5 09:29:23 2006 Subject: [Histonet] Eosin Y aqueous Message-ID: <71437982F5B13A4D9A5B2669BDB89EE4023E34A5@ISS-CL-EX-V1.soton.ac.uk> Has anyone used Aqueous Eosin Y from Sigma for H&E staining frozen sections. It always seems to leach out of the tissue - I have tried staining for between 2-10minutes (the staining is always very strong before washing). I have tried mounting in aqueous mountant (Vector) after washing with tap water (5min) and also in DPX after dehydrating in alcohol and clearing in zylene. In both instances most of the Eosin staining was washed away or leached out while the mountant was setting. I will buy some different eosin if I cant get this one to work but just wondered if anyone has encountered similar problems. Thanks Sonya From Kemlo.Rogerson <@t> waht.swest.nhs.uk Tue Dec 5 09:34:27 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Tue Dec 5 09:33:26 2006 Subject: [Histonet] Eosin Y aqueous Message-ID: <86ADE4EB583CE64799A9924684A0FBBF01246F2C@wahtntex2.waht.swest.nhs.uk> Has anyone used Aqueous Eosin Y from Sigma for H&E staining frozen sections. It always seems to leach out of the tissue - I have tried staining for between 2-10minutes (the staining is always very strong before washing). I have tried mounting in aqueous mountant (Vector) after washing with tap water (5min) and also in DPX after dehydrating in alcohol and clearing in zylene. In both instances most of the Eosin staining was washed away or leached out while the mountant was setting. I will buy some different eosin if I cant get this one to work but just wondered if anyone has encountered similar problems. Thanks Sonya Tried using calcium ions as an accentuator? Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Once you are in the orbit of your destiny, weightlessness is the only result. --Baba Amte This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From rjbuesa <@t> yahoo.com Tue Dec 5 09:56:25 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Dec 5 09:56:36 2006 Subject: [Histonet] Fwd: Clinical Laboratory Operations in a City that is 8, 300 feet Above Sea Level Message-ID: <521053.40685.qm@web61225.mail.yahoo.com> Hi Histonetter: This article may be interesting for all "high altitude" histotechs. Ren? J. Note: forwarded message attached. --------------------------------- Check out the all-new Yahoo! Mail beta - Fire up a more powerful email and get things done faster. From Michiko.Kanemitsu <@t> medecine.unige.ch Fri Dec 1 03:26:13 2006 From: Michiko.Kanemitsu <@t> medecine.unige.ch (Michiko) Date: Tue Dec 5 10:52:01 2006 Subject: [Histonet] Rat brain cryostat sections Message-ID: <5.2.1.1.2.20061201101537.00bfe210@mail.medecine.unige.ch> Hello, I have problems with rat brain cryostat section: the tissue has many holes. Here's the extraction procedures. 1: Intracardiac perfusion with 0.9% NaCl (+liquitamine) for 2-3 min. 2: Followed by cold 4% paraformaldehyde for approx. 15 min. 3: Extraction of the brain and post fix in 4% paraformaldehyde at 4?C overnight. 4: Store in PBS + azide. 5: Incubation in 30 % sucrose. 6: Rapid freezing. 7: Cryostat section 20 um. Question: Why are there many holes in brain tissue? 1: If the perfusion pressure was too high, can this break capillaries and make holes? 2: If the sucrose incubation was not sufficient, rapid freezing can destroy the tissue? 3: Or other factors? Thank you for help in advance. Michiko K From shiwei.zhai <@t> Vanderbilt.Edu Fri Dec 1 09:28:23 2006 From: shiwei.zhai <@t> Vanderbilt.Edu (Zhai, Shiwei) Date: Tue Dec 5 10:52:02 2006 Subject: [Histonet] Endothelin B receptor-C-terminus Message-ID: <14D37D96FEF55F478384252D0D062CB362290C@mailbe06.mc.vanderbilt.edu> I am using an antibody called Endothelin B receptor from Fitzgerald. It is a sheep anti rat. The secondary i used is a donkey anti sheep. I have tried with couple of dilutions and did not show up anything (no positive signals). The tissue I am working on is formalin fixed paraffin section. I did use antigen retrieval. Does any one have experience with this antibody? Thanks. Shiwei Zhai 1055 MRB4 Pediatric GI Department Vanderbilt University From rjbuesa <@t> yahoo.com Tue Dec 5 11:01:29 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Dec 5 11:01:36 2006 Subject: [Histonet] H&E slides fading in a few days In-Reply-To: <00F6A285218A0643975FE0E4F22930A40152015C@vchexmb5.vch.ca> Message-ID: <956356.99274.qm@web61223.mail.yahoo.com> Yeung: The answer is in your question: chlorine is the culprit! You can decolorize ANY stain with chlorine. You will have to eliminate the chlorine and one of the most effective ways is to heat the water. Ren? J. "Yeung, Iris [VA]" wrote: Hi, We have problem with the slides fading in a relatively short time. Our water supply is high in chlorine. We use Gill's III and alcoholic eosin. Coverslip in Entellin(?spelling) and glass coverslip. Seems to fade in small bx like prostatic needle bx than larger blocks. Any suggestion why it happens?? Yeung _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Have a burning question? Go to Yahoo! Answers and get answers from real people who know. From Karen.Heckford <@t> CHW.edu Tue Dec 5 11:22:32 2006 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Tue Dec 5 11:22:43 2006 Subject: [Histonet] Cryostat Service Message-ID: Hello Everyone, I need to find someone that services older Tissue Tek cryostats. We are located in the Bay Area, California. Cheers, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathololgy Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu From cwscouten <@t> myneurolab.com Tue Dec 5 11:52:19 2006 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Tue Dec 5 11:51:47 2006 Subject: [Histonet] Rat brain cryostat sections Message-ID: <5784D843593D874C93E9BADCB87342AB01307956@tpiserver03.Coretech-holdings.com> 1) The problem is probably not due to perfusion pressure. You would need 9 ft of gravity pressure to equal normal blood pressure. Unless you used a peristaltic pump at very high flow rate, you could not get enough pressure to do any damage. This sounds like freezing artifact, the "Swiss Cheese" aritfact due to too slow a rate of freezing. Did you store in 30% sucrose until the brain sank, indicating saturation? Maybe 3 or 4 days? What is your definition of quick freeze? Solid within 2 or 3 seconds is what is necessary. How did you achieve the freeze? A pedastal on the fast freeze rack of a cryostat is not even close, would cause the sort of problem you have described, unless throughly saturated with sucrose. Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michiko Sent: Friday, December 01, 2006 3:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Rat brain cryostat sections Hello, I have problems with rat brain cryostat section: the tissue has many holes. Here's the extraction procedures. 1: Intracardiac perfusion with 0.9% NaCl (+liquitamine) for 2-3 min. 2: Followed by cold 4% paraformaldehyde for approx. 15 min. 3: Extraction of the brain and post fix in 4% paraformaldehyde at 4?C overnight. 4: Store in PBS + azide. 5: Incubation in 30 % sucrose. 6: Rapid freezing. 7: Cryostat section 20 um. Question: Why are there many holes in brain tissue? 1: If the perfusion pressure was too high, can this break capillaries and make holes? 2: If the sucrose incubation was not sufficient, rapid freezing can destroy the tissue? 3: Or other factors? Thank you for help in advance. Michiko K _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cwscouten <@t> myneurolab.com Tue Dec 5 11:54:16 2006 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Tue Dec 5 11:53:42 2006 Subject: [Histonet] disposable cryostat object discs Message-ID: <5784D843593D874C93E9BADCB87342AB01307957@tpiserver03.Coretech-holdings.com> The plastic would not conduct the cold, and would serve as a thermal insulator. Also, it might crack with the cold. This sounds like a bad idea. Why is it percieved as necessary or useful? Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim Merriam Sent: Tuesday, December 05, 2006 7:07 AM To: Histonet Subject: [Histonet] disposable cryostat object discs Hello, Does anyone know of a vendor that sells disposable (plastic) cryostat object discs? Kim Kim Merriam Cambridge, MA ________________________________________________________________________ ____________ Any questions? Get answers on any topic at www.Answers.yahoo.com. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jcline <@t> wchsys.org Tue Dec 5 11:55:36 2006 From: jcline <@t> wchsys.org (Joyce Cline) Date: Tue Dec 5 11:55:47 2006 Subject: [Histonet] Cherly seeking source--Microtome block chuck alignment tool In-Reply-To: <968478.69568.qm@web50903.mail.yahoo.com> Message-ID: <001401c71896$91dd7800$1d2a14ac@wchsys.org> You can get this alignment tool from Marketlab (www.marketlabinc.com) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Monday, December 04, 2006 2:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] seeking source--Microtome block chuck alignment tool Hi Everyone I am looking for the vendor source for the gidget that aligns a block chuck so all the microtomes will match. It's a universal sledge with an aluminum or steel 'block' that fits into the holder to square it up in all three dimensions. Does anyone know where to find them and about how much they are these days? Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one Tech at a time. 281.883.7704 c 281.852.9457 o admin@fullstaff.org Sign up for the FREE monthly newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From brett_connolly <@t> merck.com Tue Dec 5 12:17:23 2006 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Tue Dec 5 12:17:50 2006 Subject: [Histonet] anti-thymidine kinase Message-ID: <355C35514FEAC9458F75947F5270974D013B2F46@usctmx1103.merck.com> Does anyone have a source for an anti-thymidine kinase antibody suitable for IHC on FFPE tissue. I have only found one from AbNova. Thanks, Brett Brett M. Connolly, Ph.D. Research Fellow MRL, Imaging Research Merck & Co., Inc. WP-44K PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------------ From beldorth.msu+hist <@t> gmail.com Tue Dec 5 13:30:46 2006 From: beldorth.msu+hist <@t> gmail.com (I.B.) Date: Tue Dec 5 13:30:56 2006 Subject: [Histonet] Another ISH problem - NBT/BCIP turns tissue brown Message-ID: Hi All, I am performing in Situ hybridization on sea lamprey embryos and am having yet another difficulty. This one has stumped everyone I have presented it to thus far. I hope someone on the list has a solution. I am using roughly 1Kb cRNA probes labeled with Digoxigenen (DIG), an Alkaline Phosphatase (AP) conjugated anti-DIG antibody, and Nitro-Blue Tetrazolium/5-Bromo-4-Chloro-3'-Indolyphosphate (NBT/BCIP) for the AP substrate. ISH is performed on whole mount embryos. As the finished embryos were very dark brown and difficult to visualize under the microscope, I began bleaching the embryos prior to rehydration (the embryos are stored dehydrated in methanol, beached overnight in 5:1 MeOH:H2O2, then rehydrated into phosphate buffer. See, for example, Journal of Experimental Zoology, 302B:458-468 [Kuratani et al., 2004]). After bleaching the embryos are white and translucent, making the NBT/BCIP reaction product easier to visualize. The problem, however, is that once I began bleaching I realized the dark brown color of the finished product is not inherent to the embryos themselves, but is somehow caused by the NBT/BCIP. As you know, the NBT/BCIP reaction product at pH 9.5 is a dark blue-black precipitate at the site of reaction. Apparently lower pH can cause a brown precipitate, but this does not seem to be my problem, for the following reasons 1) the brown color is not confined to the reaction site, rather it is distributed throughout the tissue 2) the brown color develops to the same extent on negative control tissue 3) embryos equilibrated through several changes of pH 9.5 solution then left overnight at that pH, then changed several more times, still produces the brown coloration (in other words, pH has nothing to do with it, and yes, I am positive the pH of the solution is 9.5) 4) incubating samples in NBT/BCIP color solution -- WITH NO probe or antibody added previously -- produces the same results 5) levamisole does not affect the results 6) I have used NBT/BCIP preparations from other companies with the same effect I have ruled out pH by incubating excessively in pH 9.5 solution. I have ruled out high background by removing probe and antibody from the equation. I have ruled out endogenous alkaline phosphatase with higher concentrations of levamisole. I have ruled out bad NBT/BCIP by using product from another company. The only factor left (that I can see) is the NBT, the BCIP, or the combination of the two. Bleaching does not seem to be the problem, as the embryos are dark after staining even without bleaching and there are numerous examples in the literature of embryos being bleached in the same manner (with excellent results). I have communicated with a member of the Kuratani lab (see journal article cited above) and they often incubate in NBT/BCIP overnight or longer and have not experience this problem. From what I can tell our methods seem identical. Has anyone experience this issue before? I need an explanation other than pH, background, endogenous AP, etc. Ion From dlawson <@t> kumc.edu Tue Dec 5 14:02:32 2006 From: dlawson <@t> kumc.edu (Donna Lawson) Date: Tue Dec 5 14:02:48 2006 Subject: [Histonet] FITC C4d Message-ID: <45757BF8020000840000DA45@kumc-smtpout.kumc.edu> HI, Is anyone out there using an indirect FITC labeled C4d antibody that is NOT an RUO? I can only find 2 and they both say for RUO not for Clinical Diagnostics. Any Help will be greatly appreciated, Donna Lawson H.T. (ASCP) QIHC Histology Supervisor University of Kansas Hospital 913-588-1134 From Rcartun <@t> harthosp.org Tue Dec 5 15:07:27 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Dec 5 15:08:08 2006 Subject: [Histonet] FITC C4d In-Reply-To: <45757BF8020000840000DA45@kumc-smtpout.kumc.edu> References: <45757BF8020000840000DA45@kumc-smtpout.kumc.edu> Message-ID: <4575993F02000077000031F4@hcnwgwds01.hh.chs> Good question. Everyone I know (including us) is using this antibody for diagnostic purposes. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Donna Lawson" 12/05/06 3:02 PM >>> HI, Is anyone out there using an indirect FITC labeled C4d antibody that is NOT an RUO? I can only find 2 and they both say for RUO not for Clinical Diagnostics. Any Help will be greatly appreciated, Donna Lawson H.T. (ASCP) QIHC Histology Supervisor University of Kansas Hospital 913-588-1134 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From mtarango <@t> nvcancer.org Tue Dec 5 16:55:10 2006 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Tue Dec 5 16:55:30 2006 Subject: [Histonet] FITC C4d Message-ID: <5AEC610C1CE02945BD63A395BA763EDEC9AEBA@NVCIEXCH02.NVCI.org> I've tried and didn't find one. It's all about the good faith effort, if you ask me. . . You have that FDA disclaimer on the reports afterall, right? Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Donna Lawson Sent: Tuesday, December 05, 2006 12:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FITC C4d HI, Is anyone out there using an indirect FITC labeled C4d antibody that is NOT an RUO? I can only find 2 and they both say for RUO not for Clinical Diagnostics. Any Help will be greatly appreciated, Donna Lawson H.T. (ASCP) QIHC Histology Supervisor University of Kansas Hospital 913-588-1134 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From Shima.Arab <@t> childrens.harvard.edu Wed Dec 6 07:46:53 2006 From: Shima.Arab <@t> childrens.harvard.edu (Arab, Shima) Date: Wed Dec 6 07:47:06 2006 Subject: [Histonet] BrdU Half-Life Message-ID: Hi Everyone, I am interested in utilizing BrdU in serum, but in order to do so, I need to know the half life. While I know the half life in vivo in rats is ~ 2hrs, does this hold true for in serum? Thanks for your help. Shima Arab Department of Cardiology Children's Hospital Boston 300 Longwood Ave Enders 1250.3 Boston, MA 02115 From joost.bruijntjes <@t> tno.nl Wed Dec 6 07:17:42 2006 From: joost.bruijntjes <@t> tno.nl (Bruijntjes, J.P. (Joost)) Date: Wed Dec 6 07:56:46 2006 Subject: [Histonet] (no subject) Message-ID: <1A7F09509ACB294B835A94B2C8EB50F402763CB6@MS-DT01VS01.tsn.tno.nl> Hi all Is anyone of you working with stem cells in order to differentiate them into neuronal cells? If so, what kind of staining procedure do you use to study them. I don't mean staining with different antibodies, but normal staining. Up to now we used HE staining, but there is not so much contrast in the eosin staining. Does anyone of you use different staining procedures? Thanks in advance Joost Bruijntjes TNO Quality and Safety Department of Toxicology and Phamacology The Netherlands TNO.NL Joost Bruijntjes T +31 30 694 44 80 F +31 30 694 49 86 E joost.bruijntjes@tno.nl Disclaimer This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html From Rcartun <@t> harthosp.org Wed Dec 6 08:45:27 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Dec 6 08:46:06 2006 Subject: [Histonet] FITC C4d In-Reply-To: <5AEC610C1CE02945BD63A395BA763EDEC9AEBA@NVCIEXCH02.NVCI.org> References: <5AEC610C1CE02945BD63A395BA763EDEC9AEBA@NVCIEXCH02.NVCI.org> Message-ID: <457691380200007700003213@hcnwgwds01.hh.chs> I thought the disclaimer was for analyte-specific reagents (ASRs) only? Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Tarango, Mark" 12/05/06 5:55 PM >>> I've tried and didn't find one. It's all about the good faith effort, if you ask me. . . You have that FDA disclaimer on the reports afterall, right? Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Donna Lawson Sent: Tuesday, December 05, 2006 12:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FITC C4d HI, Is anyone out there using an indirect FITC labeled C4d antibody that is NOT an RUO? I can only find 2 and they both say for RUO not for Clinical Diagnostics. Any Help will be greatly appreciated, Donna Lawson H.T. (ASCP) QIHC Histology Supervisor University of Kansas Hospital 913-588-1134 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From pruegg <@t> ihctech.net Wed Dec 6 08:53:43 2006 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Wed Dec 6 08:53:59 2006 Subject: [Histonet] neuro path In-Reply-To: <1A7F09509ACB294B835A94B2C8EB50F402763CB6@MS-DT01VS01.tsn.tno.nl> Message-ID: <002201c71946$535c46f0$6501a8c0@Patsy> I am also looking for a special stain to demonstrate demyelinization in rat brains and spinal cords. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bruijntjes, J.P. (Joost) Sent: Wednesday, December 06, 2006 6:18 AM To: histonet@pathology.swmed.edu Subject: [Histonet] (no subject) Hi all Is anyone of you working with stem cells in order to differentiate them into neuronal cells? If so, what kind of staining procedure do you use to study them. I don't mean staining with different antibodies, but normal staining. Up to now we used HE staining, but there is not so much contrast in the eosin staining. Does anyone of you use different staining procedures? Thanks in advance Joost Bruijntjes TNO Quality and Safety Department of Toxicology and Phamacology The Netherlands TNO.NL Joost Bruijntjes T +31 30 694 44 80 F +31 30 694 49 86 E joost.bruijntjes@tno.nl Disclaimer This e-mail and its contents are subject to the DISCLAIMER at http://www.tno.nl/disclaimer/email.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sonya.martin <@t> soton.ac.uk Wed Dec 6 09:45:18 2006 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Wed Dec 6 09:45:37 2006 Subject: [Histonet] F4-80 immunofluorescence of cells for confocal Message-ID: <71437982F5B13A4D9A5B2669BDB89EE4023E34A8@ISS-CL-EX-V1.soton.ac.uk> Hi All, Has anyone used F4-80 to label cultured macrophages. The staining should be on the cell membrane but I only get internal staining!? My protocol was as follows - I was trying to see how different staining and permeabilisation protocls affected membrane staining; 1) Label cells in culture with biotinylated F4-80 (Serotec; 1h, 37oC), wash PBS, fix 4% PFA in PBS (20min, room temp), permeabilise or not with 0.2% Triton, 2% BSA in PBS or 0.05% saponin, 2% BSA in PBS (20min room temp), incubate with streptavidin Alexa488 2) Fix cells as above, block with 2% BSA, label with F4-80, permeabilise as above, label with streptavidin Alexa488 3) Fix cells as above, permeabilise (as above) or not, label with F4-80, wash, label with streptavidin Alexa488 There was no staining on any of the cells without permeabilisation. All cells with permeabilisation were stained but all staining was intracellular. Any suggestions? From gcallis <@t> montana.edu Wed Dec 6 10:31:18 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Dec 6 10:31:33 2006 Subject: [Histonet] F4-80 immunofluorescence of cells for confocal In-Reply-To: <71437982F5B13A4D9A5B2669BDB89EE4023E34A8@ISS-CL-EX-V1.soto n.ac.uk> References: <71437982F5B13A4D9A5B2669BDB89EE4023E34A8@ISS-CL-EX-V1.soton.ac.uk> Message-ID: <6.0.0.22.1.20061206090538.01b62e00@gemini.msu.montana.edu> Since F4/80 is a cell surface marker, you don't need to permeabilize cells to achieve positive staining. I think your fixation with 4% paraformaldehyde may be too long, try 2 minutes or do a time panel starting at 2 minutes and work up. You can also do this with the fixative concentration especially for cultured cells. We never use aldehyde fixation for this marker, but it is in Histonet archives that it works on formalin fixed paraffin sections AFTER a retrieval or digestion. You may have crosslinked your antigen too strongly so it requires a longer staining time than 1 hr. We use RT for our staining protocols, and it only takes 30 minutes for this primary antibody. We would fix the cells with 75% acetone/25% absolute ethanol at RT for 5 minutes, go directly to a buffer after fixation (no more air drying). One can air dry the cultured cells before fixation, but rinse away the culture media with red indicator (it will fluoresce) and protein carrier before air drying or fixation. We have not found that BSA is not a good block with this antibody, you are better off to use 5% goat or some other normal serum (horse, swine, donkey) and add 1.25% mouse serum, use this Normal serum block is also the diluent for your biotinylated primary also (rat antimouse monoclonal). Our normal serum block is 30 minutes before applying the primary. The Strepavidin 488 should be in a serum free diluent. We prefer a buffer with a very low deteregent concentration for immunofluorescence staining. 0.025% Tween 20 to all diluents, buffers, leaving out BSA or a normal serum entirely in an immunofluorescent staining protocol with murine CD markers has proven very adequate, clean. Buffer can be either TBS or Dulbeccos PBS with 0.025% Tween 20, and we use this to dilute the Strepavidin 488, or simply use pure buffer to dilute the SA-488. Molecular Probes advises no serum in diluent with any Strepavidin Alexa Fluors. Question: are these cells supposed to be positive for F4/80, as there are other macrophage CD markers? You may need to use another antibody? Saponin is a detergent generally used for intercalating the cholesterol component of cell walls and used for intracellular immunoCYTOchemical staining for cytokines attached to the Golgi in single cells. a At 08:45 AM 12/6/2006, you wrote: >Hi All, > >Has anyone used F4-80 to label cultured macrophages. The staining should >be on the cell membrane but I only get internal staining!? My protocol >was as follows - I was trying to see how different staining and >permeabilisation protocls affected membrane staining; > >1) Label cells in culture with biotinylated F4-80 (Serotec; 1h, 37oC), >wash PBS, fix 4% PFA in PBS (20min, room temp), permeabilise or not with >0.2% Triton, 2% BSA in PBS or 0.05% saponin, 2% BSA in PBS (20min room >temp), incubate with streptavidin Alexa488 > >2) Fix cells as above, block with 2% BSA, label with F4-80, permeabilise >as above, label with streptavidin Alexa488 > >3) Fix cells as above, permeabilise (as above) or not, label with F4-80, >wash, label with streptavidin Alexa488 > >There was no staining on any of the cells without permeabilisation. All >cells with permeabilisation were stained but all staining was >intracellular. > > >Any suggestions? > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From gcallis <@t> montana.edu Wed Dec 6 10:32:57 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Wed Dec 6 10:33:17 2006 Subject: demyelinization staining Re: [Histonet] neuro path In-Reply-To: <002201c71946$535c46f0$6501a8c0@Patsy> References: <1A7F09509ACB294B835A94B2C8EB50F402763CB6@MS-DT01VS01.tsn.tno.nl> <002201c71946$535c46f0$6501a8c0@Patsy> Message-ID: <6.0.0.22.1.20061206093135.01b79008@gemini.msu.montana.edu> Kluver Barrea Luxol fast blue is a classic OR solochrome cyanin R (John Kiernal protocol). At 07:53 AM 12/6/2006, you wrote: >I am also looking for a special stain to demonstrate demyelinization in rat >brains and spinal cords. >Patsy Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From Jacqueline.Malam <@t> rli.mbht.nhs.uk Wed Dec 6 10:41:45 2006 From: Jacqueline.Malam <@t> rli.mbht.nhs.uk (Malam Jacqueline) Date: Wed Dec 6 10:46:21 2006 Subject: [Histonet] RE: decreased staining on controls Message-ID: I have noticed the same but, as I always cut my control sections fresh with the test (they go on the same slide), I am sure that it's the control blocks that deteriorate with age. Coincidentally, I did a test run today on all my CD 15 blocks in store to compare with the positive control check slides I did on the same blocks back in Sept '05 (I keep all my pos control check slides for future reference). This is because the other day I trialled some CD 15 from a different supplier (but same clone) and found that the current CD 15 pos block seemed to stain rather weaker with both my routine and new CD 15 antisera compared to the results I got for that same block in Sept '05. The results showed a reduction in the staining intensity. Happily (but not for the patient) we have just had a Hodgkin's case in which was rip-roaringly CD 15 positive so I can replenish my store. I have also noticed the same with CD 30 in the past. Jacqui malam DISCLAIMER: This e-mail is confidential and privileged. If you are not the intended recipient please accept our apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform postmaster@rli.mbht.nhs.uk that this message has gone astray before deleting it. Comments or opinions expressed in this email are those of their respective contributors only. The views expressed do not represent the views of the Trust, its management or employees. University Hospitals of Morecambe Bay NHS Trust is not responsible and disclaims any and all liability for the content of comments written within.Thank you for your co-operation. From sonya.martin <@t> soton.ac.uk Wed Dec 6 11:32:25 2006 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Wed Dec 6 11:32:38 2006 Subject: [Histonet] F4-80 immunofluorescence of cells for confocal Message-ID: <71437982F5B13A4D9A5B2669BDB89EE4023E34A9@ISS-CL-EX-V1.soton.ac.uk> Thanks, that's v helpful! The cells were positive for F4-80 by flow cytometry. Basically I want to look at F4-80 labelling along with an intracellular protein so I need to be able to permeabilise the cells without affecting the membrane staining. I will try changing the fixation protocol. Interestingly the only time I have seen good membrane staining with the F4-80 was when I labelled unfixed cells with F4-80 followed by SA-488 and THEN fixed the cells with 4% PFA. I have been using 4% PFA as most of the articles I have looked at use it and I used to use it when looking at nuclear proteins but I havent done much work with membrane proteins so I guess I just have to experiment. Thanks Sonya -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: 06 December 2006 16:31 To: Martin S.; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] F4-80 immunofluorescence of cells for confocal Since F4/80 is a cell surface marker, you don't need to permeabilize cells to achieve positive staining. I think your fixation with 4% paraformaldehyde may be too long, try 2 minutes or do a time panel starting at 2 minutes and work up. You can also do this with the fixative concentration especially for cultured cells. We never use aldehyde fixation for this marker, but it is in Histonet archives that it works on formalin fixed paraffin sections AFTER a retrieval or digestion. You may have crosslinked your antigen too strongly so it requires a longer staining time than 1 hr. We use RT for our staining protocols, and it only takes 30 minutes for this primary antibody. We would fix the cells with 75% acetone/25% absolute ethanol at RT for 5 minutes, go directly to a buffer after fixation (no more air drying). One can air dry the cultured cells before fixation, but rinse away the culture media with red indicator (it will fluoresce) and protein carrier before air drying or fixation. We have not found that BSA is not a good block with this antibody, you are better off to use 5% goat or some other normal serum (horse, swine, donkey) and add 1.25% mouse serum, use this Normal serum block is also the diluent for your biotinylated primary also (rat antimouse monoclonal). Our normal serum block is 30 minutes before applying the primary. The Strepavidin 488 should be in a serum free diluent. We prefer a buffer with a very low deteregent concentration for immunofluorescence staining. 0.025% Tween 20 to all diluents, buffers, leaving out BSA or a normal serum entirely in an immunofluorescent staining protocol with murine CD markers has proven very adequate, clean. Buffer can be either TBS or Dulbeccos PBS with 0.025% Tween 20, and we use this to dilute the Strepavidin 488, or simply use pure buffer to dilute the SA-488. Molecular Probes advises no serum in diluent with any Strepavidin Alexa Fluors. Question: are these cells supposed to be positive for F4/80, as there are other macrophage CD markers? You may need to use another antibody? Saponin is a detergent generally used for intercalating the cholesterol component of cell walls and used for intracellular immunoCYTOchemical staining for cytokines attached to the Golgi in single cells. a At 08:45 AM 12/6/2006, you wrote: >Hi All, > >Has anyone used F4-80 to label cultured macrophages. The staining >should be on the cell membrane but I only get internal staining!? My >protocol was as follows - I was trying to see how different staining >and permeabilisation protocls affected membrane staining; > >1) Label cells in culture with biotinylated F4-80 (Serotec; 1h, 37oC), >wash PBS, fix 4% PFA in PBS (20min, room temp), permeabilise or not >with 0.2% Triton, 2% BSA in PBS or 0.05% saponin, 2% BSA in PBS (20min >room temp), incubate with streptavidin Alexa488 > >2) Fix cells as above, block with 2% BSA, label with F4-80, >permeabilise as above, label with streptavidin Alexa488 > >3) Fix cells as above, permeabilise (as above) or not, label with >F4-80, wash, label with streptavidin Alexa488 > >There was no staining on any of the cells without permeabilisation. All >cells with permeabilisation were stained but all staining was >intracellular. > > >Any suggestions? > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From TJJ <@t> Stowers-Institute.org Wed Dec 6 12:02:25 2006 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Wed Dec 6 12:02:50 2006 Subject: [Histonet] Automated stainer Message-ID: One of our PI labs has asked me if there are any small automated stainers on the market. We currently have a Leica H&E Autostainer which they use to deparaffinize, counterstain, and dehydrate their IHC stained slides. They're interested in perhaps putting something in their lab, but they don't need anything as big as what we have. I'm interested only in the batch-type units, not the single slide, chain-drive ones. Thanks for any suggestions! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From HornHV <@t> archildrens.org Wed Dec 6 12:12:22 2006 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Dec 6 12:12:40 2006 Subject: [Histonet] Automated stainer In-Reply-To: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6EEF@EMAIL.archildrens.org> The Shandon Gemini is what we have. It uses 250 ml of reagents in each container which is small amount. We love our Gemini. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Johnson, Teri Sent: Wednesday, December 06, 2006 12:02 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Automated stainer One of our PI labs has asked me if there are any small automated stainers on the market. We currently have a Leica H&E Autostainer which they use to deparaffinize, counterstain, and dehydrate their IHC stained slides. They're interested in perhaps putting something in their lab, but they don't need anything as big as what we have. I'm interested only in the batch-type units, not the single slide, chain-drive ones. Thanks for any suggestions! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From gu.lang <@t> gmx.at Wed Dec 6 12:46:09 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Wed Dec 6 12:45:27 2006 Subject: [Histonet] nuclei extraction from paraffin slides Message-ID: <001901c71966$cbf41c80$c812a8c0@dielangs.at> Hi, I am looking for a good protocol for extracting nuclei from paraffin slides. The nuclei suspension should serve for FISH-testing on lymphoma-material. Perhaps someone could share his/her protocol with me. Thanks in advance Gudrun Lang Histologie Linz, Austria From mstahl <@t> bvha.org Wed Dec 6 12:58:29 2006 From: mstahl <@t> bvha.org (Stahl, Michael) Date: Wed Dec 6 12:58:03 2006 Subject: [Histonet] RE: decreased staining on controls Message-ID: <4C878E714B21EB4F8EB159777B8822EE287E9F@bvfyms01.net.bvha.org> We never cut our IHC controls ahead of time. We also found that they tend to have decreased staining the longer They are stored. And don't ya just love postive patients for controls.( I guess that's sounds bad) Michael Stahl HT (ASCP) BVRHC Findlay,Oh From tracisachs <@t> hotmail.com Wed Dec 6 13:23:58 2006 From: tracisachs <@t> hotmail.com (Traci Sachs) Date: Wed Dec 6 13:24:11 2006 Subject: [Histonet] FITC C4d In-Reply-To: <457691380200007700003213@hcnwgwds01.hh.chs> Message-ID: Donna, Quidel carries a C4d anibody ( not indirect labeled ) for FITC. The secondary is a Goat anti-mouse FITC. The item number was A213 and this worked well at a dilution of 1:40 made in normal goat serum on frozen tissue. The Quidel antibody is an ASR. >From: "Richard Cartun" >To: "Donna Lawson" , >,"Mark Tarango" >Subject: RE: [Histonet] FITC C4d >Date: Wed, 06 Dec 2006 09:45:27 -0500 > >I thought the disclaimer was for analyte-specific reagents (ASRs) only? > >Richard > >Richard W. Cartun, Ph.D. >Director, Immunopathology & Histology >Assistant Director, Anatomic Pathology >Hartford Hospital >80 Seymour Street >Hartford, CT 06102 >(860) 545-1596 >(860) 545-0174 Fax > > >>> "Tarango, Mark" 12/05/06 5:55 PM >>> >I've tried and didn't find one. It's all about the good faith effort, >if you ask me. . . > >You have that FDA disclaimer on the reports afterall, right? > > > >Mark Adam Tarango HT(ASCP) > >Histology/Immunohistochemistry Supervisor > >Nevada Cancer Institute > >One Breakthrough Way > >Las Vegas, NV 89135 > >mtarango@nvcancer.org > >Direct Line (702) 822-5112 > >Fax (702) 939-7663 > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Donna >Lawson >Sent: Tuesday, December 05, 2006 12:03 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] FITC C4d > >HI, > >Is anyone out there using an indirect FITC labeled C4d antibody that >is >NOT an RUO? I can only find 2 and they both say for RUO not for >Clinical >Diagnostics. > >Any Help will be greatly appreciated, > >Donna Lawson H.T. (ASCP) QIHC >Histology Supervisor >University of Kansas Hospital >913-588-1134 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >"EMF " made the following annotations. >------------------------------------------------------------------------------ >CONFIDENTIALITY NOTICE: This e-mail message, including any >attachments, is for the sole use of the intended >recipient(s) and may contain confidential, proprietary, >and/or privileged information protected by law. If you are >not the intended recipient, you may not use, copy, or >distribute this e-mail message or its attachments. If you >believe you have received this e-mail message in error, >please contact the sender by reply e-mail and destroy all >copies of the original message >============================================================================== > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >Confidentiality Notice > >This e-mail message, including any attachments, is for the sole use of >the intended recipient(s) and may contain confidential or proprietary >information which is legally privileged. Any unauthorized review, use, >disclosure, or distribution is prohibited. If you are not the intended >recipient, please promptly contact the sender by reply e-mail and >destroy all copies of the original message. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ WIN up to $10,000 in cash or prizes – enter the Microsoft Office Live Sweepstakes http://clk..atdmt.com/MRT/go/aub0050001581mrt/direct/01/ From tbraud <@t> holyredeemer.com Wed Dec 6 13:56:36 2006 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Wed Dec 6 13:58:26 2006 Subject: [Histonet] FITC C4d In-Reply-To: Message-ID: Ditto on Quidel's C4d performance, its great! It is listed as a RUO which is no biggie, as long as you've done your homework, documentation and have an ASR disclaimer on any patient report. It still takes interpretation and correlation with clinican results. We used it when looking at frozen section renal allografts with cell mediated rejection at 1:20 and the goat anti mouse FITC at 1:10 Terri Braud -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Traci Sachs Sent: Wednesday, December 06, 2006 2:24 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] FITC C4d Donna, Quidel carries a C4d anibody ( not indirect labeled ) for FITC. The secondary is a Goat anti-mouse FITC. The item number was A213 and this worked well at a dilution of 1:40 made in normal goat serum on frozen tissue. The Quidel antibody is an ASR. >From: "Richard Cartun" >To: "Donna Lawson" , >,"Mark Tarango" >Subject: RE: [Histonet] FITC C4d >Date: Wed, 06 Dec 2006 09:45:27 -0500 > >I thought the disclaimer was for analyte-specific reagents (ASRs) only? > >Richard > >Richard W. Cartun, Ph.D. >Director, Immunopathology & Histology >Assistant Director, Anatomic Pathology >Hartford Hospital >80 Seymour Street >Hartford, CT 06102 >(860) 545-1596 >(860) 545-0174 Fax > > >>> "Tarango, Mark" 12/05/06 5:55 PM >>> >I've tried and didn't find one. It's all about the good faith effort, >if you ask me. . . > >You have that FDA disclaimer on the reports afterall, right? > > > >Mark Adam Tarango HT(ASCP) > >Histology/Immunohistochemistry Supervisor > >Nevada Cancer Institute > >One Breakthrough Way > >Las Vegas, NV 89135 > >mtarango@nvcancer.org > >Direct Line (702) 822-5112 > >Fax (702) 939-7663 > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Donna >Lawson >Sent: Tuesday, December 05, 2006 12:03 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] FITC C4d > >HI, > >Is anyone out there using an indirect FITC labeled C4d antibody that >is >NOT an RUO? I can only find 2 and they both say for RUO not for >Clinical >Diagnostics. > >Any Help will be greatly appreciated, > >Donna Lawson H.T. (ASCP) QIHC >Histology Supervisor >University of Kansas Hospital >913-588-1134 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >"EMF " made the following annotations. >------------------------------------------------------------------------------ >CONFIDENTIALITY NOTICE: This e-mail message, including any >attachments, is for the sole use of the intended >recipient(s) and may contain confidential, proprietary, >and/or privileged information protected by law. If you are >not the intended recipient, you may not use, copy, or >distribute this e-mail message or its attachments. If you >believe you have received this e-mail message in error, >please contact the sender by reply e-mail and destroy all >copies of the original message >============================================================================== > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >Confidentiality Notice > >This e-mail message, including any attachments, is for the sole use of >the intended recipient(s) and may contain confidential or proprietary >information which is legally privileged. Any unauthorized review, use, >disclosure, or distribution is prohibited. If you are not the intended >recipient, please promptly contact the sender by reply e-mail and >destroy all copies of the original message. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ WIN up to $10,000 in cash or prizes - enter the Microsoft Office Live Sweepstakes http://clk..atdmt.com/MRT/go/aub0050001581mrt/direct/01/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. From rjbuesa <@t> yahoo.com Wed Dec 6 14:04:47 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Dec 6 14:04:56 2006 Subject: [Histonet] nuclei extraction from paraffin slides In-Reply-To: <001901c71966$cbf41c80$c812a8c0@dielangs.at> Message-ID: <20061206200448.19686.qmail@web61220.mail.yahoo.com> Gudrun: We used to so something similar (for flow cytometry) on whole prostate large specimens. First we selected (from the stained slide) the area we wanted. From that are we took a "core" biosy, reembedded it and section at 200 ?m steps. Those thick slices were treated with xylene (and hydrated to saline solution) to eliminate the paraffin. Later they were disintegrated by shaking them in saline solution until the components were dislogged. That was the material used for flow cytometry and the results were good. Hope this will help you! Ren? J. Gudrun Lang wrote: Hi, I am looking for a good protocol for extracting nuclei from paraffin slides. The nuclei suspension should serve for FISH-testing on lymphoma-material. Perhaps someone could share his/her protocol with me. Thanks in advance Gudrun Lang Histologie Linz, Austria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From tim.morken <@t> thermofisher.com Wed Dec 6 14:34:01 2006 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Wed Dec 6 14:34:18 2006 Subject: RUO's in diagnostics... Was...RE: [Histonet] FITC C4d In-Reply-To: Message-ID: Terri wrote "It is listed as a RUO which is no biggie, as long as you've done your homework, documentation and have an ASR disclaimer on any patient report." This is not true. ASR's and RUO's are not the same. ASR's are made ONLY by FDA-registered companies and they can be used for diagnostic tests once the lab validates them. RUO's are often made by non-FDA-registered companies and cannot be used for diagnostic work (by law). The law actually says that an RUO-only, non-FDA-registered company is not supposed to sell to diagnostic labs if the lab intends to use the product for diagnostic test (reported and charged tests). If it is used only for research and is not reported or charged, then there is no problem. The lab is required to use ONLY ASR or IVD labelled products for diagnostic tests for which they report the results and charge for. RUO's by ANY company are not to be used for diagnostic test. The disclaimer is ONLY for ASR's. There is no disclaimer for RUO antibodies because RUO's are not to be used for diagnostics, at least they are not to be reported or charged. This is an FDA rule and they say the CAP is wrong to say a diagnostic lab can use RUO's (CAP has a section in the checklist that says an RUO can be used if the lab cannot find the antibody as an ASR or IVD. FDA says this is not correct and no use of RUO for diagnostics is legal). Of course these rules are flouted daily by thousands of labs and FDA has made no effort to enforce this them. Still, it is the law. Below is my correspondence with FDA about this subject. Tim Morken Product Development Lab Vision - Neomarkers ThermoFisher Scientific Here is my question to FDA and their answer. I asked in 2004 and confirmed this in 2006. "Recently (Dec 2004, (http://www.cap.org/apps/docs/laboratory_accreditation/checklists/checkl istf tp.html) The College of American Pathologists (CAP), the accrediting agency for diagnostic Anatomic Pathology labs, determined that RUO antibody reagents are suitable for diagnostic work as long as the laboratory using them makes a "reasonable" effort to find such antibodies in IVD or ASR format, and undergoes validation of such antibodies: from: Anatomic Pathology checklist, Sept 2004, Item # ANP.12425 "Antibodies, nucleic acid sequences, etc., labeled "Research Use Only" (RUO) purchased from commercial sources may be used in home brew tests only if the laboratory has made a reasonable effort to search for IVD or ASR class reagents. The results of that failed search should be documented by the laboratory director."" My question is: Does the FDA approve of this reversal of the policy to not allow RUO's for diagnostic use. -----Original Message----- From: Gutman, Steve [mailto:SIG@CDRH.FDA.GOV] Sent: Tuesday, April 05, 2005 8:14 AM To: 'tpmorken@labvision.com' Cc: St. Pierre, Don J.; Rodgers, Anthony (CDRH); Cardamone, Thomas E.; Yost, Judith A (CMS) Subject: FW: 03-393 aqr DSMICA Email Form Response Dear Mr. Morken, I was not familiar with this CAP change and will share this with CMS the organization that grants deemed status to CAP. While I do think this is a pragmatic approach and probably well intentioned, I also think unfortunately it is clearly at odds with the law. Companies or laboratories that follow this are potentially in jeopardy of compliance action. If you have questions or concerns, please feel free to call me at 240-328-0484. Steven Gutman, M.D. Director, Office of In Vitro Diagnostics -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Wednesday, December 06, 2006 11:57 AM To: Histonet (E-mail) Subject: RE: [Histonet] FITC C4d Ditto on Quidel's C4d performance, its great! It is listed as a RUO which is no biggie, as long as you've done your homework, documentation and have an ASR disclaimer on any patient report. It still takes interpretation and correlation with clinican results. We used it when looking at frozen section renal allografts with cell mediated rejection at 1:20 and the goat anti mouse FITC at 1:10 Terri Braud -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Traci Sachs Sent: Wednesday, December 06, 2006 2:24 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] FITC C4d Donna, Quidel carries a C4d anibody ( not indirect labeled ) for FITC. The secondary is a Goat anti-mouse FITC. The item number was A213 and this worked well at a dilution of 1:40 made in normal goat serum on frozen tissue. The Quidel antibody is an ASR. >From: "Richard Cartun" >To: "Donna Lawson" , >,"Mark Tarango" > >Subject: RE: [Histonet] FITC C4d >Date: Wed, 06 Dec 2006 09:45:27 -0500 > >I thought the disclaimer was for analyte-specific reagents (ASRs) only? > >Richard > >Richard W. Cartun, Ph.D. >Director, Immunopathology & Histology >Assistant Director, Anatomic Pathology >Hartford Hospital >80 Seymour Street >Hartford, CT 06102 >(860) 545-1596 >(860) 545-0174 Fax > > >>> "Tarango, Mark" 12/05/06 5:55 PM >>> >I've tried and didn't find one. It's all about the good faith effort, >if you ask me. . . > >You have that FDA disclaimer on the reports afterall, right? > > > >Mark Adam Tarango HT(ASCP) > >Histology/Immunohistochemistry Supervisor > >Nevada Cancer Institute > >One Breakthrough Way > >Las Vegas, NV 89135 > >mtarango@nvcancer.org > >Direct Line (702) 822-5112 > >Fax (702) 939-7663 > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Donna >Lawson >Sent: Tuesday, December 05, 2006 12:03 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] FITC C4d > >HI, > >Is anyone out there using an indirect FITC labeled C4d antibody that is >NOT an RUO? I can only find 2 and they both say for RUO not for >Clinical Diagnostics. > >Any Help will be greatly appreciated, > >Donna Lawson H.T. (ASCP) QIHC >Histology Supervisor >University of Kansas Hospital >913-588-1134 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >"EMF " made the following annotations. >----------------------------------------------------------------------- >------- CONFIDENTIALITY NOTICE: This e-mail message, including any >attachments, is for the sole use of the intended >recipient(s) and may contain confidential, proprietary, and/or >privileged information protected by law. If you are not the intended >recipient, you may not use, copy, or distribute this e-mail message or >its attachments. If you believe you have received this e-mail message >in error, please contact the sender by reply e-mail and destroy all >copies of the original message >======================================================================= >======= > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >Confidentiality Notice > >This e-mail message, including any attachments, is for the sole use of >the intended recipient(s) and may contain confidential or proprietary >information which is legally privileged. Any unauthorized review, use, >disclosure, or distribution is prohibited. If you are not the intended >recipient, please promptly contact the sender by reply e-mail and >destroy all copies of the original message. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ WIN up to $10,000 in cash or prizes - enter the Microsoft Office Live Sweepstakes http://clk..atdmt.com/MRT/go/aub0050001581mrt/direct/01/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tbraud <@t> holyredeemer.com Wed Dec 6 15:20:20 2006 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Wed Dec 6 15:22:14 2006 Subject: RUO's in diagnostics... Was...RE: [Histonet] FITC C4d In-Reply-To: Message-ID: For Pete's sake, if even the FDA calls it a "pragmatic approach", and seems to turn its back to laboratories' compliance, what is a poor supervisor lost in the world of pathology request's to do???? Terri -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Morken, Tim Sent: Wednesday, December 06, 2006 3:34 PM To: histonet@lists.utsouthwestern.edu Subject: RUO's in diagnostics... Was...RE: [Histonet] FITC C4d Terri wrote "It is listed as a RUO which is no biggie, as long as you've done your homework, documentation and have an ASR disclaimer on any patient report." This is not true. ASR's and RUO's are not the same. ASR's are made ONLY by FDA-registered companies and they can be used for diagnostic tests once the lab validates them. RUO's are often made by non-FDA-registered companies and cannot be used for diagnostic work (by law). The law actually says that an RUO-only, non-FDA-registered company is not supposed to sell to diagnostic labs if the lab intends to use the product for diagnostic test (reported and charged tests). If it is used only for research and is not reported or charged, then there is no problem. The lab is required to use ONLY ASR or IVD labelled products for diagnostic tests for which they report the results and charge for. RUO's by ANY company are not to be used for diagnostic test. The disclaimer is ONLY for ASR's. There is no disclaimer for RUO antibodies because RUO's are not to be used for diagnostics, at least they are not to be reported or charged. This is an FDA rule and they say the CAP is wrong to say a diagnostic lab can use RUO's (CAP has a section in the checklist that says an RUO can be used if the lab cannot find the antibody as an ASR or IVD. FDA says this is not correct and no use of RUO for diagnostics is legal). Of course these rules are flouted daily by thousands of labs and FDA has made no effort to enforce this them. Still, it is the law. Below is my correspondence with FDA about this subject. Tim Morken Product Development Lab Vision - Neomarkers ThermoFisher Scientific Here is my question to FDA and their answer. I asked in 2004 and confirmed this in 2006. "Recently (Dec 2004, (http://www.cap.org/apps/docs/laboratory_accreditation/checklists/checkl istf tp.html) The College of American Pathologists (CAP), the accrediting agency for diagnostic Anatomic Pathology labs, determined that RUO antibody reagents are suitable for diagnostic work as long as the laboratory using them makes a "reasonable" effort to find such antibodies in IVD or ASR format, and undergoes validation of such antibodies: from: Anatomic Pathology checklist, Sept 2004, Item # ANP.12425 "Antibodies, nucleic acid sequences, etc., labeled "Research Use Only" (RUO) purchased from commercial sources may be used in home brew tests only if the laboratory has made a reasonable effort to search for IVD or ASR class reagents. The results of that failed search should be documented by the laboratory director."" My question is: Does the FDA approve of this reversal of the policy to not allow RUO's for diagnostic use. -----Original Message----- From: Gutman, Steve [mailto:SIG@CDRH.FDA.GOV] Sent: Tuesday, April 05, 2005 8:14 AM To: 'tpmorken@labvision.com' Cc: St. Pierre, Don J.; Rodgers, Anthony (CDRH); Cardamone, Thomas E.; Yost, Judith A (CMS) Subject: FW: 03-393 aqr DSMICA Email Form Response Dear Mr. Morken, I was not familiar with this CAP change and will share this with CMS the organization that grants deemed status to CAP. While I do think this is a pragmatic approach and probably well intentioned, I also think unfortunately it is clearly at odds with the law. Companies or laboratories that follow this are potentially in jeopardy of compliance action. If you have questions or concerns, please feel free to call me at 240-328-0484. Steven Gutman, M.D. Director, Office of In Vitro Diagnostics -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Wednesday, December 06, 2006 11:57 AM To: Histonet (E-mail) Subject: RE: [Histonet] FITC C4d Ditto on Quidel's C4d performance, its great! It is listed as a RUO which is no biggie, as long as you've done your homework, documentation and have an ASR disclaimer on any patient report. It still takes interpretation and correlation with clinican results. We used it when looking at frozen section renal allografts with cell mediated rejection at 1:20 and the goat anti mouse FITC at 1:10 Terri Braud -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Traci Sachs Sent: Wednesday, December 06, 2006 2:24 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] FITC C4d Donna, Quidel carries a C4d anibody ( not indirect labeled ) for FITC. The secondary is a Goat anti-mouse FITC. The item number was A213 and this worked well at a dilution of 1:40 made in normal goat serum on frozen tissue. The Quidel antibody is an ASR. >From: "Richard Cartun" >To: "Donna Lawson" , >,"Mark Tarango" > >Subject: RE: [Histonet] FITC C4d >Date: Wed, 06 Dec 2006 09:45:27 -0500 > >I thought the disclaimer was for analyte-specific reagents (ASRs) only? > >Richard > >Richard W. Cartun, Ph.D. >Director, Immunopathology & Histology >Assistant Director, Anatomic Pathology >Hartford Hospital >80 Seymour Street >Hartford, CT 06102 >(860) 545-1596 >(860) 545-0174 Fax > > >>> "Tarango, Mark" 12/05/06 5:55 PM >>> >I've tried and didn't find one. It's all about the good faith effort, >if you ask me. . . > >You have that FDA disclaimer on the reports afterall, right? > > > >Mark Adam Tarango HT(ASCP) > >Histology/Immunohistochemistry Supervisor > >Nevada Cancer Institute > >One Breakthrough Way > >Las Vegas, NV 89135 > >mtarango@nvcancer.org > >Direct Line (702) 822-5112 > >Fax (702) 939-7663 > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Donna >Lawson >Sent: Tuesday, December 05, 2006 12:03 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] FITC C4d > >HI, > >Is anyone out there using an indirect FITC labeled C4d antibody that is >NOT an RUO? I can only find 2 and they both say for RUO not for >Clinical Diagnostics. > >Any Help will be greatly appreciated, > >Donna Lawson H.T. (ASCP) QIHC >Histology Supervisor >University of Kansas Hospital >913-588-1134 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >"EMF " made the following annotations. >----------------------------------------------------------------------- >------- CONFIDENTIALITY NOTICE: This e-mail message, including any >attachments, is for the sole use of the intended >recipient(s) and may contain confidential, proprietary, and/or >privileged information protected by law. If you are not the intended >recipient, you may not use, copy, or distribute this e-mail message or >its attachments. If you believe you have received this e-mail message >in error, please contact the sender by reply e-mail and destroy all >copies of the original message >======================================================================= >======= > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >Confidentiality Notice > >This e-mail message, including any attachments, is for the sole use of >the intended recipient(s) and may contain confidential or proprietary >information which is legally privileged. Any unauthorized review, use, >disclosure, or distribution is prohibited. If you are not the intended >recipient, please promptly contact the sender by reply e-mail and >destroy all copies of the original message. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ WIN up to $10,000 in cash or prizes - enter the Microsoft Office Live Sweepstakes http://clk..atdmt.com/MRT/go/aub0050001581mrt/direct/01/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. From HornHV <@t> archildrens.org Wed Dec 6 15:48:41 2006 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Wed Dec 6 15:49:13 2006 Subject: RUO's in diagnostics... Was...RE: [Histonet] FITC C4d In-Reply-To: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6EF5@EMAIL.archildrens.org> We do not charge for any RUO's. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Wednesday, December 06, 2006 3:20 PM To: Histonet (E-mail) Subject: RE: RUO's in diagnostics... Was...RE: [Histonet] FITC C4d For Pete's sake, if even the FDA calls it a "pragmatic approach", and seems to turn its back to laboratories' compliance, what is a poor supervisor lost in the world of pathology request's to do???? Terri -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Morken, Tim Sent: Wednesday, December 06, 2006 3:34 PM To: histonet@lists.utsouthwestern.edu Subject: RUO's in diagnostics... Was...RE: [Histonet] FITC C4d Terri wrote "It is listed as a RUO which is no biggie, as long as you've done your homework, documentation and have an ASR disclaimer on any patient report." This is not true. ASR's and RUO's are not the same. ASR's are made ONLY by FDA-registered companies and they can be used for diagnostic tests once the lab validates them. RUO's are often made by non-FDA-registered companies and cannot be used for diagnostic work (by law). The law actually says that an RUO-only, non-FDA-registered company is not supposed to sell to diagnostic labs if the lab intends to use the product for diagnostic test (reported and charged tests). If it is used only for research and is not reported or charged, then there is no problem. The lab is required to use ONLY ASR or IVD labelled products for diagnostic tests for which they report the results and charge for. RUO's by ANY company are not to be used for diagnostic test. The disclaimer is ONLY for ASR's. There is no disclaimer for RUO antibodies because RUO's are not to be used for diagnostics, at least they are not to be reported or charged. This is an FDA rule and they say the CAP is wrong to say a diagnostic lab can use RUO's (CAP has a section in the checklist that says an RUO can be used if the lab cannot find the antibody as an ASR or IVD. FDA says this is not correct and no use of RUO for diagnostics is legal). Of course these rules are flouted daily by thousands of labs and FDA has made no effort to enforce this them. Still, it is the law. Below is my correspondence with FDA about this subject. Tim Morken Product Development Lab Vision - Neomarkers ThermoFisher Scientific Here is my question to FDA and their answer. I asked in 2004 and confirmed this in 2006. "Recently (Dec 2004, (http://www.cap.org/apps/docs/laboratory_accreditation/checklists/checkl istf tp.html) The College of American Pathologists (CAP), the accrediting agency for diagnostic Anatomic Pathology labs, determined that RUO antibody reagents are suitable for diagnostic work as long as the laboratory using them makes a "reasonable" effort to find such antibodies in IVD or ASR format, and undergoes validation of such antibodies: from: Anatomic Pathology checklist, Sept 2004, Item # ANP.12425 "Antibodies, nucleic acid sequences, etc., labeled "Research Use Only" (RUO) purchased from commercial sources may be used in home brew tests only if the laboratory has made a reasonable effort to search for IVD or ASR class reagents. The results of that failed search should be documented by the laboratory director."" My question is: Does the FDA approve of this reversal of the policy to not allow RUO's for diagnostic use. -----Original Message----- From: Gutman, Steve [mailto:SIG@CDRH.FDA.GOV] Sent: Tuesday, April 05, 2005 8:14 AM To: 'tpmorken@labvision.com' Cc: St. Pierre, Don J.; Rodgers, Anthony (CDRH); Cardamone, Thomas E.; Yost, Judith A (CMS) Subject: FW: 03-393 aqr DSMICA Email Form Response Dear Mr. Morken, I was not familiar with this CAP change and will share this with CMS the organization that grants deemed status to CAP. While I do think this is a pragmatic approach and probably well intentioned, I also think unfortunately it is clearly at odds with the law. Companies or laboratories that follow this are potentially in jeopardy of compliance action. If you have questions or concerns, please feel free to call me at 240-328-0484. Steven Gutman, M.D. Director, Office of In Vitro Diagnostics -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Wednesday, December 06, 2006 11:57 AM To: Histonet (E-mail) Subject: RE: [Histonet] FITC C4d Ditto on Quidel's C4d performance, its great! It is listed as a RUO which is no biggie, as long as you've done your homework, documentation and have an ASR disclaimer on any patient report. It still takes interpretation and correlation with clinican results. We used it when looking at frozen section renal allografts with cell mediated rejection at 1:20 and the goat anti mouse FITC at 1:10 Terri Braud -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Traci Sachs Sent: Wednesday, December 06, 2006 2:24 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] FITC C4d Donna, Quidel carries a C4d anibody ( not indirect labeled ) for FITC. The secondary is a Goat anti-mouse FITC. The item number was A213 and this worked well at a dilution of 1:40 made in normal goat serum on frozen tissue. The Quidel antibody is an ASR. >From: "Richard Cartun" >To: "Donna Lawson" , >,"Mark Tarango" > >Subject: RE: [Histonet] FITC C4d >Date: Wed, 06 Dec 2006 09:45:27 -0500 > >I thought the disclaimer was for analyte-specific reagents (ASRs) only? > >Richard > >Richard W. Cartun, Ph.D. >Director, Immunopathology & Histology >Assistant Director, Anatomic Pathology >Hartford Hospital >80 Seymour Street >Hartford, CT 06102 >(860) 545-1596 >(860) 545-0174 Fax > > >>> "Tarango, Mark" 12/05/06 5:55 PM >>> >I've tried and didn't find one. It's all about the good faith effort, >if you ask me. . . > >You have that FDA disclaimer on the reports afterall, right? > > > >Mark Adam Tarango HT(ASCP) > >Histology/Immunohistochemistry Supervisor > >Nevada Cancer Institute > >One Breakthrough Way > >Las Vegas, NV 89135 > >mtarango@nvcancer.org > >Direct Line (702) 822-5112 > >Fax (702) 939-7663 > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Donna >Lawson >Sent: Tuesday, December 05, 2006 12:03 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] FITC C4d > >HI, > >Is anyone out there using an indirect FITC labeled C4d antibody that is >NOT an RUO? I can only find 2 and they both say for RUO not for >Clinical Diagnostics. > >Any Help will be greatly appreciated, > >Donna Lawson H.T. (ASCP) QIHC >Histology Supervisor >University of Kansas Hospital >913-588-1134 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >"EMF " made the following annotations. >----------------------------------------------------------------------- >------- CONFIDENTIALITY NOTICE: This e-mail message, including any >attachments, is for the sole use of the intended >recipient(s) and may contain confidential, proprietary, and/or >privileged information protected by law. If you are not the intended >recipient, you may not use, copy, or distribute this e-mail message or >its attachments. If you believe you have received this e-mail message >in error, please contact the sender by reply e-mail and destroy all >copies of the original message >======================================================================= >======= > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >Confidentiality Notice > >This e-mail message, including any attachments, is for the sole use of >the intended recipient(s) and may contain confidential or proprietary >information which is legally privileged. Any unauthorized review, use, >disclosure, or distribution is prohibited. If you are not the intended >recipient, please promptly contact the sender by reply e-mail and >destroy all copies of the original message. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ WIN up to $10,000 in cash or prizes - enter the Microsoft Office Live Sweepstakes http://clk..atdmt.com/MRT/go/aub0050001581mrt/direct/01/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From tim.morken <@t> thermofisher.com Wed Dec 6 15:56:52 2006 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Wed Dec 6 15:57:36 2006 Subject: RUO's in diagnostics... Was...RE: [Histonet] FITC C4d In-Reply-To: Message-ID: Terri, yes, it is crazy.CLIA enforcement arms (including CAP) have ignored this for years, but FDA has not changed it's stance. This affects antibody vendors as well. Why go the the trouble and expense of FDA registration (which requires a quality control system and FDA inspections) if RUO-only companies can sell to the same labs, not have any quality control, no FDA oversight, and no consequences? Apparently CAP simply made up their RUO policy and never even asked FDA about it. I guess you can do as everyone else does: ignore it. Tim -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Wednesday, December 06, 2006 1:20 PM To: Histonet (E-mail) Subject: RE: RUO's in diagnostics... Was...RE: [Histonet] FITC C4d For Pete's sake, if even the FDA calls it a "pragmatic approach", and seems to turn its back to laboratories' compliance, what is a poor supervisor lost in the world of pathology request's to do???? Terri -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Morken, Tim Sent: Wednesday, December 06, 2006 3:34 PM To: histonet@lists.utsouthwestern.edu Subject: RUO's in diagnostics... Was...RE: [Histonet] FITC C4d Terri wrote "It is listed as a RUO which is no biggie, as long as you've done your homework, documentation and have an ASR disclaimer on any patient report." This is not true. ASR's and RUO's are not the same. ASR's are made ONLY by FDA-registered companies and they can be used for diagnostic tests once the lab validates them. RUO's are often made by non-FDA-registered companies and cannot be used for diagnostic work (by law). The law actually says that an RUO-only, non-FDA-registered company is not supposed to sell to diagnostic labs if the lab intends to use the product for diagnostic test (reported and charged tests). If it is used only for research and is not reported or charged, then there is no problem. The lab is required to use ONLY ASR or IVD labelled products for diagnostic tests for which they report the results and charge for. RUO's by ANY company are not to be used for diagnostic test. The disclaimer is ONLY for ASR's. There is no disclaimer for RUO antibodies because RUO's are not to be used for diagnostics, at least they are not to be reported or charged. This is an FDA rule and they say the CAP is wrong to say a diagnostic lab can use RUO's (CAP has a section in the checklist that says an RUO can be used if the lab cannot find the antibody as an ASR or IVD. FDA says this is not correct and no use of RUO for diagnostics is legal). Of course these rules are flouted daily by thousands of labs and FDA has made no effort to enforce this them. Still, it is the law. Below is my correspondence with FDA about this subject. Tim Morken Product Development Lab Vision - Neomarkers ThermoFisher Scientific Here is my question to FDA and their answer. I asked in 2004 and confirmed this in 2006. "Recently (Dec 2004, (http://www.cap.org/apps/docs/laboratory_accreditation/checklists/checkl istf tp.html) The College of American Pathologists (CAP), the accrediting agency for diagnostic Anatomic Pathology labs, determined that RUO antibody reagents are suitable for diagnostic work as long as the laboratory using them makes a "reasonable" effort to find such antibodies in IVD or ASR format, and undergoes validation of such antibodies: from: Anatomic Pathology checklist, Sept 2004, Item # ANP.12425 "Antibodies, nucleic acid sequences, etc., labeled "Research Use Only" (RUO) purchased from commercial sources may be used in home brew tests only if the laboratory has made a reasonable effort to search for IVD or ASR class reagents. The results of that failed search should be documented by the laboratory director."" My question is: Does the FDA approve of this reversal of the policy to not allow RUO's for diagnostic use. -----Original Message----- From: Gutman, Steve [mailto:SIG@CDRH.FDA.GOV] Sent: Tuesday, April 05, 2005 8:14 AM To: 'tpmorken@labvision.com' Cc: St. Pierre, Don J.; Rodgers, Anthony (CDRH); Cardamone, Thomas E.; Yost, Judith A (CMS) Subject: FW: 03-393 aqr DSMICA Email Form Response Dear Mr. Morken, I was not familiar with this CAP change and will share this with CMS the organization that grants deemed status to CAP. While I do think this is a pragmatic approach and probably well intentioned, I also think unfortunately it is clearly at odds with the law. Companies or laboratories that follow this are potentially in jeopardy of compliance action. If you have questions or concerns, please feel free to call me at 240-328-0484. Steven Gutman, M.D. Director, Office of In Vitro Diagnostics -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Terri Braud Sent: Wednesday, December 06, 2006 11:57 AM To: Histonet (E-mail) Subject: RE: [Histonet] FITC C4d Ditto on Quidel's C4d performance, its great! It is listed as a RUO which is no biggie, as long as you've done your homework, documentation and have an ASR disclaimer on any patient report. It still takes interpretation and correlation with clinican results. We used it when looking at frozen section renal allografts with cell mediated rejection at 1:20 and the goat anti mouse FITC at 1:10 Terri Braud -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Traci Sachs Sent: Wednesday, December 06, 2006 2:24 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] FITC C4d Donna, Quidel carries a C4d anibody ( not indirect labeled ) for FITC. The secondary is a Goat anti-mouse FITC. The item number was A213 and this worked well at a dilution of 1:40 made in normal goat serum on frozen tissue. The Quidel antibody is an ASR. >From: "Richard Cartun" >To: "Donna Lawson" , >,"Mark Tarango" > >Subject: RE: [Histonet] FITC C4d >Date: Wed, 06 Dec 2006 09:45:27 -0500 > >I thought the disclaimer was for analyte-specific reagents (ASRs) only? > >Richard > >Richard W. Cartun, Ph.D. >Director, Immunopathology & Histology >Assistant Director, Anatomic Pathology >Hartford Hospital >80 Seymour Street >Hartford, CT 06102 >(860) 545-1596 >(860) 545-0174 Fax > > >>> "Tarango, Mark" 12/05/06 5:55 PM >>> >I've tried and didn't find one. It's all about the good faith effort, >if you ask me. . . > >You have that FDA disclaimer on the reports afterall, right? > > > >Mark Adam Tarango HT(ASCP) > >Histology/Immunohistochemistry Supervisor > >Nevada Cancer Institute > >One Breakthrough Way > >Las Vegas, NV 89135 > >mtarango@nvcancer.org > >Direct Line (702) 822-5112 > >Fax (702) 939-7663 > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Donna >Lawson >Sent: Tuesday, December 05, 2006 12:03 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] FITC C4d > >HI, > >Is anyone out there using an indirect FITC labeled C4d antibody that is >NOT an RUO? I can only find 2 and they both say for RUO not for >Clinical Diagnostics. > >Any Help will be greatly appreciated, > >Donna Lawson H.T. (ASCP) QIHC >Histology Supervisor >University of Kansas Hospital >913-588-1134 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >"EMF " made the following annotations. >----------------------------------------------------------------------- >------- CONFIDENTIALITY NOTICE: This e-mail message, including any >attachments, is for the sole use of the intended >recipient(s) and may contain confidential, proprietary, and/or >privileged information protected by law. If you are not the intended >recipient, you may not use, copy, or distribute this e-mail message or >its attachments. If you believe you have received this e-mail message >in error, please contact the sender by reply e-mail and destroy all >copies of the original message >======================================================================= >======= > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >Confidentiality Notice > >This e-mail message, including any attachments, is for the sole use of >the intended recipient(s) and may contain confidential or proprietary >information which is legally privileged. Any unauthorized review, use, >disclosure, or distribution is prohibited. If you are not the intended >recipient, please promptly contact the sender by reply e-mail and >destroy all copies of the original message. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ WIN up to $10,000 in cash or prizes - enter the Microsoft Office Live Sweepstakes http://clk..atdmt.com/MRT/go/aub0050001581mrt/direct/01/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Wed Dec 6 16:19:25 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Dec 6 16:21:23 2006 Subject: [Histonet] Rat brain cryostat sections Message-ID: My experience though indicates that slow freezing my not be the culprit. Normal Saline seems to perfuse quickly into the tissue causing excessive ingress of water into cells. Normal Saline, despite what one would think, is not osmotically neutral with cells. We had the same problem with brain tissue for frozen section. When tissue arrived in saline, large freezing like holes appeared. When tissue was received on moistened card, the artifact disappeared. Use a culture medium if you can and the chance of this freezing artifact (or osmotic artifact) may indeed go away. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles Scouten Sent: Wednesday, 6 December 2006 4:52 AM To: Michiko; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Rat brain cryostat sections 1) The problem is probably not due to perfusion pressure. You would need 9 ft of gravity pressure to equal normal blood pressure. Unless you used a peristaltic pump at very high flow rate, you could not get enough pressure to do any damage. This sounds like freezing artifact, the "Swiss Cheese" aritfact due to too slow a rate of freezing. Did you store in 30% sucrose until the brain sank, indicating saturation? Maybe 3 or 4 days? What is your definition of quick freeze? Solid within 2 or 3 seconds is what is necessary. How did you achieve the freeze? A pedastal on the fast freeze rack of a cryostat is not even close, would cause the sort of problem you have described, unless throughly saturated with sucrose. Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michiko Sent: Friday, December 01, 2006 3:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Rat brain cryostat sections Hello, I have problems with rat brain cryostat section: the tissue has many holes. Here's the extraction procedures. 1: Intracardiac perfusion with 0.9% NaCl (+liquitamine) for 2-3 min. 2: Followed by cold 4% paraformaldehyde for approx. 15 min. 3: Extraction of the brain and post fix in 4% paraformaldehyde at 4?C overnight. 4: Store in PBS + azide. 5: Incubation in 30 % sucrose. 6: Rapid freezing. 7: Cryostat section 20 um. Question: Why are there many holes in brain tissue? 1: If the perfusion pressure was too high, can this break capillaries and make holes? 2: If the sucrose incubation was not sufficient, rapid freezing can destroy the tissue? 3: Or other factors? Thank you for help in advance. Michiko K _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From amartinez <@t> carisdx.com Wed Dec 6 16:59:06 2006 From: amartinez <@t> carisdx.com (Martinez, Angela) Date: Wed Dec 6 17:00:09 2006 Subject: [Histonet] Employment Opportunity Message-ID: <9B8A3AC772C7F64680392A7CB8FBFB0F0155DB8D@s-irv-ex301.PathologyPartners.intranet> Caris would like to announce a new job opening in the Phoenix Arizona location. The position is for a full time Histotechnologist. This position will entail routine Histology including: specimen processing, embedding, microtomy, staining, and immunohistochemistry. Candidates should be HT certified and should possess a BS degree. Further, candidates should have 1-3 years experience in embedding and sectioning of surgical specimens in a certified Histopathology laboratory. Excellent communication skills and the ability to work in a high volume environment is required. To apply, please e-mail resume to: Email: amartine@carisdx.com From amylee779 <@t> yahoo.com Wed Dec 6 18:17:45 2006 From: amylee779 <@t> yahoo.com (Amy Lee) Date: Wed Dec 6 18:44:35 2006 Subject: [Histonet] fibronectin IHC Message-ID: <956006.84734.qm@web58307.mail.re3.yahoo.com> Hello, I was asked to do Fibronectin IHC on FFPE rat tissue. Does anybody know a good antibody for this purpose? Thanks in advance, Amy --------------------------------- Any questions? Get answers on any topic at Yahoo! Answers. Try it now. From Vickroy.Jim <@t> mhsil.com Thu Dec 7 08:46:55 2006 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Thu Dec 7 08:47:27 2006 Subject: [Histonet] paraffin problem Message-ID: Lately we are experiencing a problem with our paraffin blocks. We use Richard Allen Type 9 and Type 1. The tissue doesn't seem to ribbon very well and at times the sections have some small striations, almost like knife lines. We have checked the disposable knives and have eliminated that variable. We use a recycled clearing agent but have eliminated that also by using fresh chemicals. We have several tissue processors and there is a possibility that the problem could be a processor issue however the majority of us think that it is a paraffin problem. We do not see any "grit" in the paraffins. We are thinking of trying a different paraffin and wondered if anyone has switched to one of the newer paraffins that work great. Obviously I can get as many samples as I would like but want to see if anyone has any suggestions or ideas that would help us eliminate some of the steps of the process. Thanks. Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From doug <@t> ppspath.com Thu Dec 7 09:16:45 2006 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Thu Dec 7 09:14:09 2006 Subject: [Histonet] paraffin problem In-Reply-To: Message-ID: Jim, I have been through many types of paraffin. I now use Paraplast plus for infiltration and the Paraplast xtra for embedding. The xtra is the best ribboning paraffin that I have found. There may be others comparable but I do not have the time to try any more out. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Thursday, December 07, 2006 9:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] paraffin problem Lately we are experiencing a problem with our paraffin blocks. We use Richard Allen Type 9 and Type 1. The tissue doesn't seem to ribbon very well and at times the sections have some small striations, almost like knife lines. We have checked the disposable knives and have eliminated that variable. We use a recycled clearing agent but have eliminated that also by using fresh chemicals. We have several tissue processors and there is a possibility that the problem could be a processor issue however the majority of us think that it is a paraffin problem. We do not see any "grit" in the paraffins. We are thinking of trying a different paraffin and wondered if anyone has switched to one of the newer paraffins that work great. Obviously I can get as many samples as I would like but want to see if anyone has any suggestions or ideas that would help us eliminate some of the steps of the process. Thanks. Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From slappycraw <@t> yahoo.com Thu Dec 7 09:26:44 2006 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Thu Dec 7 09:27:00 2006 Subject: [Histonet] paraffin problem Message-ID: <20061207152645.41058.qmail@web53602.mail.yahoo.com> I agree with paraplast, over the years it has always been my choice for overall performance in a paraffin --------------------------------- Everyone is raving about the all-new Yahoo! Mail beta. From gcallis <@t> montana.edu Thu Dec 7 09:31:00 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Dec 7 09:31:20 2006 Subject: [Histonet] paraffin problem In-Reply-To: References: Message-ID: <6.0.0.22.1.20061207082426.01b39d40@gemini.msu.montana.edu> Stir your paraffin BEFORE you embed, a cheap kitchen spatula works for us. Paraffins contain plastic polymers and other additives that settle out when paraffin is melted. Redistribution of additives will help sectioning and get rid of some funky "grit". We don't use RA paraffins, but have noticed this with another brand IF we fail to stir before embedding. Clean your embedding paraffin reservoir regularly. During processing, the agitation keeps paraffin stirred up. At 07:46 AM 12/7/2006, you wrote: > > >Lately we are experiencing a problem with our paraffin blocks. We use >Richard Allen Type 9 and Type 1. The tissue doesn't seem to ribbon >very well and at times the sections have some small striations, almost >like knife lines. We have checked the disposable knives and have >eliminated that variable. We use a recycled clearing agent but have >eliminated that also by using fresh chemicals. We have several tissue >processors and there is a possibility that the problem could be a >processor issue however the majority of us think that it is a paraffin >problem. We do not see any "grit" in the paraffins. We are thinking of >trying a different paraffin and wondered if anyone has switched to one >of the newer paraffins that work great. Obviously I can get as many >samples as I would like but want to see if anyone has any suggestions or >ideas that would help us eliminate some of the steps of the process. >Thanks. > > > >Jim Vickroy > >Technical Supervisor - Surgical and Autopsy Pathology > >Memorial Medical Center > > > > > >This message (including any attachments) contains confidential information >intended for a >specific individual and purpose, and is protected by law. If you are not >the intended recipient, >you should delete this message. Any disclosure, copying, or distribution >of this message, or the >taking of any action based on it, is strictly prohibited. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From cwscouten <@t> myneurolab.com Thu Dec 7 10:10:41 2006 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Thu Dec 7 10:10:09 2006 Subject: [Histonet] Rat brain cryostat sections Message-ID: <5784D843593D874C93E9BADCB87342AB01307980@tpiserver03.Coretech-holdings.com> Yes, when metabolism stops, and the sodium and other pumps shut off, sodium runs in to the cell down the gradient, even if isotonic saline is used, and the interior of the cell becomes hypertonic. Water must enter to balance osmolarity. That is why cells swell in response to almost any challenge that shuts down metabolism. I have discussed this in an article in the May 2006 issue of Microscopy Today. Soaking in 30% (very hypertonic) sucrose should have "dehydrated" those cells, though. Yes, they might have already burst if saline was continued for too long before fixative arrived. When you find what works, please let us know. The solution will reveal the cause. Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: Tony Henwood [mailto:AnthonyH@chw.edu.au] Sent: Wednesday, December 06, 2006 4:19 PM To: Charles Scouten; Michiko; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Rat brain cryostat sections My experience though indicates that slow freezing my not be the culprit. Normal Saline seems to perfuse quickly into the tissue causing excessive ingress of water into cells. Normal Saline, despite what one would think, is not osmotically neutral with cells. We had the same problem with brain tissue for frozen section. When tissue arrived in saline, large freezing like holes appeared. When tissue was received on moistened card, the artifact disappeared. Use a culture medium if you can and the chance of this freezing artifact (or osmotic artifact) may indeed go away. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Charles Scouten Sent: Wednesday, 6 December 2006 4:52 AM To: Michiko; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Rat brain cryostat sections 1) The problem is probably not due to perfusion pressure. You would need 9 ft of gravity pressure to equal normal blood pressure. Unless you used a peristaltic pump at very high flow rate, you could not get enough pressure to do any damage. This sounds like freezing artifact, the "Swiss Cheese" aritfact due to too slow a rate of freezing. Did you store in 30% sucrose until the brain sank, indicating saturation? Maybe 3 or 4 days? What is your definition of quick freeze? Solid within 2 or 3 seconds is what is necessary. How did you achieve the freeze? A pedastal on the fast freeze rack of a cryostat is not even close, would cause the sort of problem you have described, unless throughly saturated with sucrose. Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michiko Sent: Friday, December 01, 2006 3:26 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Rat brain cryostat sections Hello, I have problems with rat brain cryostat section: the tissue has many holes. Here's the extraction procedures. 1: Intracardiac perfusion with 0.9% NaCl (+liquitamine) for 2-3 min. 2: Followed by cold 4% paraformaldehyde for approx. 15 min. 3: Extraction of the brain and post fix in 4% paraformaldehyde at 4?C overnight. 4: Store in PBS + azide. 5: Incubation in 30 % sucrose. 6: Rapid freezing. 7: Cryostat section 20 um. Question: Why are there many holes in brain tissue? 1: If the perfusion pressure was too high, can this break capillaries and make holes? 2: If the sucrose incubation was not sufficient, rapid freezing can destroy the tissue? 3: Or other factors? Thank you for help in advance. Michiko K _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From gentras <@t> vetmed.auburn.edu Thu Dec 7 10:19:20 2006 From: gentras <@t> vetmed.auburn.edu (Atoska Gentry) Date: Thu Dec 7 10:19:41 2006 Subject: [Histonet] neurospheres Message-ID: <45783F08.3040408@vetmed.auburn.edu> Hello, does anyone have a protocol for fixation & staining neurospheres from tissue culture either on chamber slides or cryosections? If so will you be so kind as to share it with me ASAP? One of our researchers here has attempted using the chamber slides but, she said the cells all washed off. Technique / protocol suggestions will be much appreciated. Thanks, Atoska -- Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu From pruegg <@t> ihctech.net Thu Dec 7 10:43:41 2006 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Thu Dec 7 10:44:07 2006 Subject: [Histonet] fibronectin IHC In-Reply-To: <956006.84734.qm@web58307.mail.re3.yahoo.com> Message-ID: <200612071643.kB7GhgB8041284@pro12.abac.com> Amy, I think the u OF Iowa Hybridoma Bank carries fibronectin, try them first, also I am pretty sure Santa Cruz has it, I used this but it has been some time ago when I was at UCHSC, I will check my files when I get back to work Friday. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Amy Lee Sent: Wednesday, December 06, 2006 5:18 PM To: histonet Subject: [Histonet] fibronectin IHC Hello, I was asked to do Fibronectin IHC on FFPE rat tissue. Does anybody know a good antibody for this purpose? Thanks in advance, Amy --------------------------------- Any questions? Get answers on any topic at Yahoo! Answers. Try it now. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Thu Dec 7 10:40:59 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Dec 7 11:01:12 2006 Subject: [Histonet] paraffin problem In-Reply-To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E273BC4@LSRIEXCH1.lsmaster.lifespan.org> You can rule out particulate matter in the paraffin by filtering some of it and comparing the results to the unfiltered product. Just put a filter paper in a funnel as usual, put it in a flask, fill the filter paper with paraffin pellets and leave the whole thing in the paraffin oven overnight. When I started in histology (a few years ago) this was standard practice because the paraffin as purchased (it came in 5 lb slabs which we had to break up with a hammer) had all kinds of impurities in it. You wouldn't believe the amount of stuff we would catch on the filter paper. From Doug.Geddes <@t> lhsc.on.ca Thu Dec 7 11:07:57 2006 From: Doug.Geddes <@t> lhsc.on.ca (Doug Geddes) Date: Thu Dec 7 11:12:25 2006 Subject: [Histonet] Polymers?? Message-ID: <4578041D020000610000022C@lhscgwaio.lhsc.on.ca> Hi Histonet, Anyone care to share experiences with polymers (universal preferred) in a clinical setting. Doug Geddes BSc, MLT Dept of Pathology LHSC London ON Canada ----------------------------------------- This information is directed in confidence solely to the person named above and may contain confidential and/or privileged material. This information may not otherwise be distributed, copied or disclosed. If you have received this e-mail in error, please notify the sender immediately via a return e-mail and destroy original message. Thank you for your cooperation. From Doug.Geddes <@t> lhsc.on.ca Thu Dec 7 10:22:33 2006 From: Doug.Geddes <@t> lhsc.on.ca (Doug Geddes) Date: Thu Dec 7 11:15:18 2006 Subject: [Histonet] Polymer Feedback? Message-ID: <4577F9790200006100000223@lhscgwaio.lhsc.on.ca> Dear Histonet, Looking for feedback and experiences with different ploymers (preferably universal) in a clinical setting. Doug Geddes BSc, MLT Dept of Pathology LHSC London ON Canada ----------------------------------------- This information is directed in confidence solely to the person named above and may contain confidential and/or privileged material. This information may not otherwise be distributed, copied or disclosed. If you have received this e-mail in error, please notify the sender immediately via a return e-mail and destroy original message. Thank you for your cooperation. From rjbuesa <@t> yahoo.com Thu Dec 7 11:15:18 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Dec 7 11:15:34 2006 Subject: [Histonet] paraffin problem In-Reply-To: Message-ID: <471007.39792.qm@web61221.mail.yahoo.com> Jim: In case you want to keep a score of answers/preferences, I alwys used Paraplast (Plus for infiltraiton, and Extra for embedding). Ren? J. "Vickroy, Jim" wrote: Lately we are experiencing a problem with our paraffin blocks. We use Richard Allen Type 9 and Type 1. The tissue doesn't seem to ribbon very well and at times the sections have some small striations, almost like knife lines. We have checked the disposable knives and have eliminated that variable. We use a recycled clearing agent but have eliminated that also by using fresh chemicals. We have several tissue processors and there is a possibility that the problem could be a processor issue however the majority of us think that it is a paraffin problem. We do not see any "grit" in the paraffins. We are thinking of trying a different paraffin and wondered if anyone has switched to one of the newer paraffins that work great. Obviously I can get as many samples as I would like but want to see if anyone has any suggestions or ideas that would help us eliminate some of the steps of the process. Thanks. Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Check out the all-new Yahoo! Mail beta - Fire up a more powerful email and get things done faster. From Doug.Geddes <@t> lhsc.on.ca Thu Dec 7 11:47:32 2006 From: Doug.Geddes <@t> lhsc.on.ca (Doug Geddes) Date: Thu Dec 7 11:49:45 2006 Subject: [Histonet] Polymer Feedback? In-Reply-To: <6.0.0.22.1.20061207103623.01b90738@gemini.msu.montana.edu> References: <4577F9790200006100000223@lhscgwaio.lhsc.on.ca> <6.0.0.22.1.20061207103623.01b90738@gemini.msu.montana.edu> Message-ID: <45780D640200006100000230@lhscgwaio.lhsc.on.ca> Sorry, polymers for IHC staining. Doug >>> Gayle Callis 2006-12-07 12:36 PM >>> Doug, Polymers for what purpose, embedding tissues? At 09:22 AM 12/7/2006, you wrote: >Dear Histonet, > >Looking for feedback and experiences with different ploymers >(preferably universal) in a clinical setting. > >Doug Geddes BSc, MLT >Dept of Pathology >LHSC London ON >Canada Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 ----------------------------------------- This information is directed in confidence solely to the person named above and may contain confidential and/or privileged material. This information may not otherwise be distributed, copied or disclosed. If you have received this e-mail in error, please notify the sender immediately via a return e-mail and destroy original message. Thank you for your cooperation. From Jerry <@t> ralambusa.com Thu Dec 7 12:22:16 2006 From: Jerry <@t> ralambusa.com (Jerry Helisek) Date: Thu Dec 7 12:22:37 2006 Subject: [Histonet] Automated stainer (Johnson, Teri) Message-ID: <3855F92002259948A66A8CA2D16E3A4F017BB3@server.ralambusa.com> Teri, Please see: http://www.ralamb.net/product_info.php?products_id=595&osCsid=52e93ef809 10bcc854ff5818dc168ef6 I think this may be what you are looking for in a small stainer (RA Lamb's StainMate) Jerry Helisek 5409 Lumley Road, Unit #102 Durham, North Carolina 27703 Phone: 919-957-1964 Fax: 919-957-1972 Cell: 919-264-7964 jerry@ralambusa.com www.ralamb.com The information contained in this e-mail message may be privileged, confidential and protected from disclosure. If you are not the intended recipient, any dissemination, distribution or copying is strictly prohibited. If you think that you have received this e-mail message in error please e-mail the sender and delete the message. Thank you. From GDawson <@t> dynacaremilwaukee.com Thu Dec 7 12:24:51 2006 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Thu Dec 7 12:32:29 2006 Subject: [Histonet] Polymer Feedback? Message-ID: Doug, Polymers Rock!!! No avidin/biotin blocking. Better sensitivity so you save some of their extra cost by taking antibody titers out farther. Clean. Fewer steps. So on and so on. Only bad thing is the cost but nothing is perfect. Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Doug Geddes Sent: Thursday, December 07, 2006 11:48 AM To: Histonet@lists.utsouthwestern.edu; Gayle Callis Subject: Re: [Histonet] Polymer Feedback? Sorry, polymers for IHC staining. Doug >>> Gayle Callis 2006-12-07 12:36 PM >>> Doug, Polymers for what purpose, embedding tissues? At 09:22 AM 12/7/2006, you wrote: >Dear Histonet, > >Looking for feedback and experiences with different ploymers >(preferably universal) in a clinical setting. > >Doug Geddes BSc, MLT >Dept of Pathology >LHSC London ON >Canada Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 ----------------------------------------- This information is directed in confidence solely to the person named above and may contain confidential and/or privileged material. This information may not otherwise be distributed, copied or disclosed. If you have received this e-mail in error, please notify the sender immediately via a return e-mail and destroy original message. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rsrichmond <@t> aol.com Thu Dec 7 12:36:53 2006 From: rsrichmond <@t> aol.com (rsrichmond@aol.com) Date: Thu Dec 7 12:37:23 2006 Subject: [Histonet] Re: paraffin problem In-Reply-To: <200612070948.5da457829c1389@rly-xl05.mx.aol.com> References: <200612070948.5da457829c1389@rly-xl05.mx.aol.com> Message-ID: <8C8E845CD1DB9FA-250-41C1@MBLK-M23.sysops.aol.com> Several people comment on problems with present day embedding waxes ("paraffin"). Geezer time. In the more than forty years I've been a pathologist, one of the biggest improvements in technology has been the replacement of simple paraffin waxes with the present proprietary mixtures. In the 1960's, many laboratories simply bought Gulfwax - the wax used by home canners to seal canning jars. For quite a few years now complex mixtures of who knows what have replaced simple paraffin. These mixtures are all proprietary and trade-secret, and as far as I know this whole change has been largely undocumented in the literature. But the improvement in sections that has resulted has been revolutionary. In the 1960's many laboratories were simply unable to cut an interpretable section of a lymph node. Frank Foote, then the chief of surgical pathology at Memorial/Sloan Kettering in New York city, often observed that most of a surgical pathology consultant's expertise was in interpreting nearly unreadable slides. Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From bwhitaker <@t> brownpathology.com Thu Dec 7 13:06:39 2006 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Thu Dec 7 13:02:30 2006 Subject: [Histonet] Polymer Feedback? In-Reply-To: Message-ID: <000a01c71a32$d30055d0$3601a8c0@brownpathology.net> Like a boss used to tell me: You can have better quality, you can have a faster turnaround time or you can produce a product that is cheaper. Pick 2. You can never have all three! Polymers are faster and better (my favorite pick for the 2) Bonnie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Thursday, December 07, 2006 12:25 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Polymer Feedback? Doug, Polymers Rock!!! No avidin/biotin blocking. Better sensitivity so you save some of their extra cost by taking antibody titers out farther. Clean. Fewer steps. So on and so on. Only bad thing is the cost but nothing is perfect. Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Doug Geddes Sent: Thursday, December 07, 2006 11:48 AM To: Histonet@lists.utsouthwestern.edu; Gayle Callis Subject: Re: [Histonet] Polymer Feedback? Sorry, polymers for IHC staining. Doug >>> Gayle Callis 2006-12-07 12:36 PM >>> Doug, Polymers for what purpose, embedding tissues? At 09:22 AM 12/7/2006, you wrote: >Dear Histonet, > >Looking for feedback and experiences with different ploymers >(preferably universal) in a clinical setting. > >Doug Geddes BSc, MLT >Dept of Pathology >LHSC London ON >Canada Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 ----------------------------------------- This information is directed in confidence solely to the person named above and may contain confidential and/or privileged material. This information may not otherwise be distributed, copied or disclosed. If you have received this e-mail in error, please notify the sender immediately via a return e-mail and destroy original message. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Thu Dec 7 13:45:07 2006 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Dec 7 13:45:26 2006 Subject: [Histonet] Polymer Feedback? In-Reply-To: <4577F9790200006100000223@lhscgwaio.lhsc.on.ca> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6EFF@EMAIL.archildrens.org> I'm interested in polymers as well. This is new for me. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Doug Geddes Sent: Thursday, December 07, 2006 10:23 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Polymer Feedback? Dear Histonet, Looking for feedback and experiences with different ploymers (preferably universal) in a clinical setting. Doug Geddes BSc, MLT Dept of Pathology LHSC London ON Canada ----------------------------------------- This information is directed in confidence solely to the person named above and may contain confidential and/or privileged material. This information may not otherwise be distributed, copied or disclosed. If you have received this e-mail in error, please notify the sender immediately via a return e-mail and destroy original message. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From int09018 <@t> alphahunt.com Thu Dec 7 14:27:13 2006 From: int09018 <@t> alphahunt.com (HCS) Date: Thu Dec 7 14:25:41 2006 Subject: [Histonet] looking for someone to do a Brucella suis IHC on Caribou Message-ID: <000601c71a3e$16ca4e00$6701a8c0@hp> Hi, I would like to find someone who does IHC that could do a Brucella suis stain on a Caribou. Please email me if you do this stain or know of a contact for me. Thanks LeRoy Brown HT(ASCP) HTL HCS, Inc. 360-966-7300 From int09018 <@t> alphahunt.com Thu Dec 7 14:29:14 2006 From: int09018 <@t> alphahunt.com (HCS) Date: Thu Dec 7 14:27:36 2006 Subject: [Histonet] looking for a slide carrier for Shandon X-Y stainer Message-ID: <000601c71a3e$5cf1e640$6701a8c0@hp> Does anyone have an extra slide carrier that fits the Shandon x-y stainer? thanks LeRoy Brown Ht(ASCP) Htl HCS, Inc. 360-966-7300 From pruegg <@t> ihctech.net Thu Dec 7 14:54:10 2006 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Thu Dec 7 14:54:24 2006 Subject: [Histonet] Polymer Feedback? In-Reply-To: <000a01c71a32$d30055d0$3601a8c0@brownpathology.net> Message-ID: <200612072054.kB7KsBen082197@pro12.abac.com> Bonnie, You are generous, my boss always said "pick one" out of the three you mentioned. In the case of labeled polymer I think you are right, they are faster and I think better, at least I do not have to worry about endogenous biotin issues. They an't cheap though. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonnie Whitaker Sent: Thursday, December 07, 2006 12:07 PM To: 'Dawson, Glen'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Polymer Feedback? Like a boss used to tell me: You can have better quality, you can have a faster turnaround time or you can produce a product that is cheaper. Pick 2. You can never have all three! Polymers are faster and better (my favorite pick for the 2) Bonnie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Thursday, December 07, 2006 12:25 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Polymer Feedback? Doug, Polymers Rock!!! No avidin/biotin blocking. Better sensitivity so you save some of their extra cost by taking antibody titers out farther. Clean. Fewer steps. So on and so on. Only bad thing is the cost but nothing is perfect. Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Doug Geddes Sent: Thursday, December 07, 2006 11:48 AM To: Histonet@lists.utsouthwestern.edu; Gayle Callis Subject: Re: [Histonet] Polymer Feedback? Sorry, polymers for IHC staining. Doug >>> Gayle Callis 2006-12-07 12:36 PM >>> Doug, Polymers for what purpose, embedding tissues? At 09:22 AM 12/7/2006, you wrote: >Dear Histonet, > >Looking for feedback and experiences with different ploymers >(preferably universal) in a clinical setting. > >Doug Geddes BSc, MLT >Dept of Pathology >LHSC London ON >Canada Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 ----------------------------------------- This information is directed in confidence solely to the person named above and may contain confidential and/or privileged material. This information may not otherwise be distributed, copied or disclosed. If you have received this e-mail in error, please notify the sender immediately via a return e-mail and destroy original message. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Thu Dec 7 14:57:26 2006 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Thu Dec 7 14:57:45 2006 Subject: [Histonet] Re: paraffin problem In-Reply-To: <8C8E845CD1DB9FA-250-41C1@MBLK-M23.sysops.aol.com> Message-ID: <200612072057.kB7KvRp5083970@pro12.abac.com> I agree Samurai, the better paraffin and disposable knives have changed paraffin sectioning forever for better. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of rsrichmond@aol.com Sent: Thursday, December 07, 2006 11:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: paraffin problem Several people comment on problems with present day embedding waxes ("paraffin"). Geezer time. In the more than forty years I've been a pathologist, one of the biggest improvements in technology has been the replacement of simple paraffin waxes with the present proprietary mixtures. In the 1960's, many laboratories simply bought Gulfwax - the wax used by home canners to seal canning jars. For quite a few years now complex mixtures of who knows what have replaced simple paraffin. These mixtures are all proprietary and trade-secret, and as far as I know this whole change has been largely undocumented in the literature. But the improvement in sections that has resulted has been revolutionary. In the 1960's many laboratories were simply unable to cut an interpretable section of a lymph node. Frank Foote, then the chief of surgical pathology at Memorial/Sloan Kettering in New York city, often observed that most of a surgical pathology consultant's expertise was in interpreting nearly unreadable slides. Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Thu Dec 7 14:45:08 2006 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Thu Dec 7 15:05:06 2006 Subject: [Histonet] Polymer Feedback? In-Reply-To: <4577F9790200006100000223@lhscgwaio.lhsc.on.ca> Message-ID: <200612072045.kB7Kj8H3077358@pro12.abac.com> Do you mean labeled polymers for ihc? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Doug Geddes Sent: Thursday, December 07, 2006 9:23 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Polymer Feedback? Dear Histonet, Looking for feedback and experiences with different ploymers (preferably universal) in a clinical setting. Doug Geddes BSc, MLT Dept of Pathology LHSC London ON Canada ----------------------------------------- This information is directed in confidence solely to the person named above and may contain confidential and/or privileged material. This information may not otherwise be distributed, copied or disclosed. If you have received this e-mail in error, please notify the sender immediately via a return e-mail and destroy original message. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kjohnson <@t> covx.com Thu Dec 7 15:09:01 2006 From: kjohnson <@t> covx.com (Kimberly Johnson) Date: Thu Dec 7 15:09:23 2006 Subject: [Histonet] Shur/mark slides In-Reply-To: <5784D843593D874C93E9BADCB87342AB01307980@tpiserver03.Coretech-holdings.com> Message-ID: Hello! I finally got myself an automated slide labeler! YAY! But I need some help on what slides to use. I have the Shur/mark labeler from TBS, and got some slides, but I realized I needed to get some positively charged slides. I know a lot of you use this machine and was wondering if you all could guide me towards a good slide that is charged that works with the labeler. Thank you so much for your time, your advise is always great! Kim Kimberly Johnson Research Associate II CovX Ph. (858)964-2050 Fax (858)964-2090 The information contained in this e-mail message may be privileged and confidential information and is intended only for the use of the individual and/or entity identified in the alias address of this message. If the reader of this message is not the intended recipient, or an employee or agent responsible to deliver it to the intended recipient, you are hereby requested not to distribute or copy this communication. If you have received this communication in error, please notify us immediately by telephone or return e-mail and delete the original message from your system. From rnishi <@t> uci.edu Thu Dec 7 15:34:17 2006 From: rnishi <@t> uci.edu (Rebecca Nishi) Date: Thu Dec 7 15:34:28 2006 Subject: [Histonet] GFP and B-Gal positive control slides Message-ID: <4B87DFCC-BE93-449B-85A0-CCF5C15AA67C@uci.edu> Does anyone know where I can buy, or can suggest an easy way to make positive control slides for GFP and B-Gal. We have use AAV contstructs with either GFP or LAC-Z and injected into mice. We have sectioned the tissue and would like to look for positive GFP or B-Gal. It would be nice to have a positive control slide. We plan on visualizing both with and without antibody amplification. I would appreciate any suggestions. Thank You. Rebecca ------------------------------------ Rebecca Nishi UC Irvine Christopher Reeve Foundation SCI Core/Anderson Lab 1226 GNRF Irvine, CA 92697-4540 rnishi@uci.edu Phone/Fax: 949-824-9728 From koellinr <@t> amgen.com Thu Dec 7 16:05:03 2006 From: koellinr <@t> amgen.com (Koelling, Ray) Date: Thu Dec 7 16:05:23 2006 Subject: [Histonet] GFP and B-Gal positive control slides Message-ID: <16834C6DFFA6004C88DE4507FB8AE544059839E9@wa-mb4-sea.amgen.com> Rebecca, For GFP, that can be a can of worms. For B-Gal this is what we do. From mouse vendor, get a Tie-2/lacZ transgenic mouse strain. Are readily available commercially. Tie-2 is expressed in endothelium and the lacZ reporter gene is co-expressed there. Take any tissue (kid/liver/whatever). B-Gal antibodies show beautifully stained endothelium. Ray Raymond Koelling Research Scientist Amgen Seattle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rebecca Nishi Sent: Thursday, December 07, 2006 1:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GFP and B-Gal positive control slides Does anyone know where I can buy, or can suggest an easy way to make positive control slides for GFP and B-Gal. We have use AAV contstructs with either GFP or LAC-Z and injected into mice. We have sectioned the tissue and would like to look for positive GFP or B-Gal. It would be nice to have a positive control slide. We plan on visualizing both with and without antibody amplification. I would appreciate any suggestions. Thank You. Rebecca ------------------------------------ Rebecca Nishi UC Irvine Christopher Reeve Foundation SCI Core/Anderson Lab 1226 GNRF Irvine, CA 92697-4540 rnishi@uci.edu Phone/Fax: 949-824-9728 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histotech <@t> charter.net Thu Dec 7 17:59:38 2006 From: histotech <@t> charter.net (histotech@charter.net) Date: Thu Dec 7 17:59:46 2006 Subject: [Histonet] Fading Message-ID: <1064608487.1165535978746.JavaMail.root@fepweb05> Hello, For the past few weeks, we've been having problems with our slides fading approximately one week after they are stained. Any ideas on what's causing this and how to correct the situation? Thank you, DDietz Morristown-Hamblen Lab From mtarango <@t> nvcancer.org Thu Dec 7 18:38:40 2006 From: mtarango <@t> nvcancer.org (Tarango, Mark) Date: Thu Dec 7 18:39:17 2006 Subject: [Histonet] neurospheres Message-ID: <5AEC610C1CE02945BD63A395BA763EDEE009BE@NVCIEXCH02.NVCI.org> When I take the chamber/divider thing off the slide, I make sure to remove the media first (and very carefully so the cells don't come off the slide). Then I make sure to let it air dry for plenty of time before I do anything to it. If she is trying to use chamber slides on a cell culture, I hope they are adherent cells. If they are the kind of cells that grow in suspension (like hematopoietic cells), then you won't many cells adhere. I don't know if neurospheres will adhere... Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Atoska Gentry Sent: Thursday, December 07, 2006 8:19 AM To: Histonet Subject: [Histonet] neurospheres Hello, does anyone have a protocol for fixation & staining neurospheres from tissue culture either on chamber slides or cryosections? If so will you be so kind as to share it with me ASAP? One of our researchers here has attempted using the chamber slides but, she said the cells all washed off. Technique / protocol suggestions will be much appreciated. Thanks, Atoska -- Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== From wulan <@t> med.kobe-u.ac.jp Thu Dec 7 19:45:21 2006 From: wulan <@t> med.kobe-u.ac.jp (Wulan Anggrahini) Date: Thu Dec 7 19:45:38 2006 Subject: [Histonet] ICAM-1 Antibody Message-ID: <001a01c71a6a$85cc4200$0bc8a8c0@your56ab121f6e> Hi.. I need information about good purified rat anti mouse ICAM-1 monoclonal antibody. I want to buy from Chemicon but there are apparently two types of clones from Chemicon for rat antimouse ICAM-1. CLone KAT-1 and Clone LTF653. Does anyone has any experience on using this antibody ? I need to stain mouse vascular tissue on frozen section with 4% Paraformaldehyde fixation. Thanks a lot for the help. Regards, Wulan Anggrahini Kobe University From debbiekeith <@t> cox.net Thu Dec 7 21:11:44 2006 From: debbiekeith <@t> cox.net (Debbie Keith) Date: Thu Dec 7 21:11:56 2006 Subject: [Histonet] cryostat whisperer???... (i need parts for a Leica, STAT) Message-ID: <5.2.0.9.0.20061207195737.00ba7908@pop.central.cox.net> i'd like to thank everyone for their input about my recent cryostat issues... the biomedical repair-folk came out on wednesday and diagnosed the issue. it seems the screw/lever on the chuck assembly (that tightens the chuck) has been over-tightened to the point of stripping the threads and rendering it chronically "un-tight". the harder it's tightened... the harder it has to be tightened. one of the repair-folk actually had what MUST have been a "Viola Moment". he tightened the handle and it sectioned like a spanky-new machine would. it was beautiful. after he left, i cut the next block... and had to adjust the chuck. keep in mind that this repair guy is a STRAPPING person that could EASILY haul large farm equipment with the best of them.... and i have the upper body strength of the average 3 year-old. after much crying, causing ice crystals to form and a hush to fall... i called the repair guy. he told me it was going to take 2-4 weeks for the parts to come from Leica. is there anyone out there that KNOWS where to get the parts quicker? i would like to note... the repair guy is pretty good. he doesn't make much conversation... but he "listens" to the machine. he said, without even looking at the machine, "i listen for the sound the machine makes when it's cutting 'right'." later, when it WAS cutting right... he said "do you hear that?? huh? do you hear THAT???". it DID make a very distinct sound. next time you make a beautiful section... listen for the "sssschhhuctt" sound. THEN, think of me crying into my cm1510. (seriously, any ideas about parts?) debbie -- No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.1.409 / Virus Database: 268.15.15/579 - Release Date: 12/7/2006 From Kemlo.Rogerson <@t> waht.swest.nhs.uk Fri Dec 8 02:22:36 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Fri Dec 8 02:21:39 2006 Subject: [Histonet] Fading Message-ID: <86ADE4EB583CE64799A9924684A0FBBF01246F79@wahtntex2.waht.swest.nhs.uk> Would be useful to know which stain is fading. I assume H&E and in that case which element, the 'H' or the 'E'? Causes are different. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Once you are in the orbit of your destiny, weightlessness is the only result. --Baba Amte This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From rjbuesa <@t> yahoo.com Fri Dec 8 07:29:50 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Dec 8 07:30:01 2006 Subject: [Histonet] Fading In-Reply-To: <1064608487.1165535978746.JavaMail.root@fepweb05> Message-ID: <747142.68162.qm@web61217.mail.yahoo.com> Even when theoretically after dehydration is completed all the water should have been removed from the section, IF the tap water used after the hamoxylin is "reach" in chlorine, those ions will react with the dyes and will make them to fave even after the sections have been coverslipped. Check for chlorine in your tap water supply. Ren? J. histotech@charter.net wrote: Hello, For the past few weeks, we've been having problems with our slides fading approximately one week after they are stained. Any ideas on what's causing this and how to correct the situation? Thank you, DDietz Morristown-Hamblen Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Everyone is raving about the all-new Yahoo! Mail beta. From bakevictoria <@t> gmail.com Fri Dec 8 08:29:10 2006 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Fri Dec 8 08:29:21 2006 Subject: [Histonet] Microscope slide trays -- not folders Message-ID: <4f016b690612080629h1f035e45od6501842298f395@mail.gmail.com> Happy Friday Everyone! Thanks to everyone who responded to my e-mail about American Histo Labs, it was a big help. Now, I'm searching for slide trays that are about 14"long and 11-1/2" wide. Some are wood others are an acrylic I think. They have 4 rows and hold a lot of slides. I've been trying to find them through my usual sources without success. Any information is welcomed. Have a nice weekend. Vikki From HornHV <@t> archildrens.org Fri Dec 8 09:07:53 2006 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Dec 8 09:08:13 2006 Subject: [Histonet] Microscope slide trays -- not folders In-Reply-To: <4f016b690612080629h1f035e45od6501842298f395@mail.gmail.com> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6F01@EMAIL.archildrens.org> Try www.brainlab.com They have them. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Friday, December 08, 2006 8:29 AM To: Histo Net list server Subject: [Histonet] Microscope slide trays -- not folders Happy Friday Everyone! Thanks to everyone who responded to my e-mail about American Histo Labs, it was a big help. Now, I'm searching for slide trays that are about 14"long and 11-1/2" wide. Some are wood others are an acrylic I think. They have 4 rows and hold a lot of slides. I've been trying to find them through my usual sources without success. Any information is welcomed. Have a nice weekend. Vikki _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From petepath <@t> yahoo.com Fri Dec 8 09:18:58 2006 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Fri Dec 8 09:19:09 2006 Subject: [Histonet] cryostat whisperer???... (i need parts for a Leica, STAT) Message-ID: <466458.27471.qm@web30404.mail.mud.yahoo.com> Dear Debbie, Try my friends at Belair Instrument Company. 973 912 8900. They sell and service these and I expect they would have something in stock to help you. Stephen Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com From Eric.C.Kellar <@t> questdiagnostics.com Fri Dec 8 09:35:52 2006 From: Eric.C.Kellar <@t> questdiagnostics.com (Kellar, Eric C) Date: Fri Dec 8 09:39:16 2006 Subject: [Histonet] Shur/mark slides Message-ID: <6843061CE6B98E4B96590D4F299618F801583BF0@qdcws0117.us.qdx.com> Kim, I have two Shur/mark slides etchers that run all day long. Most Colorfrost Plus slides in darker colors - blue/green/tan available from Cardinal, Thermo Fisher, Lamb all work well. I have also tried Shur/mark brand Colorfrost Plus slides, they come with a color painted background on the opposite side of the slide to enhance visibility. They are very nice, but are extremely overpriced. It's the diamond stylus that needs to be replaced often with any frosted slide you choose. They're expensive too and come in a five pack, so stock up. Eric C. Kellar Quest Diagnostics, Inc Histology Laboratory Supervisor Miami -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kimberly Johnson Sent: Thursday, December 07, 2006 4:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Shur/mark slides Hello! I finally got myself an automated slide labeler! YAY! But I need some help on what slides to use. I have the Shur/mark labeler from TBS, and got some slides, but I realized I needed to get some positively charged slides. I know a lot of you use this machine and was wondering if you all could guide me towards a good slide that is charged that works with the labeler. Thank you so much for your time, your advise is always great! Kim Kimberly Johnson Research Associate II CovX Ph. (858)964-2050 Fax (858)964-2090 The information contained in this e-mail message may be privileged and confidential information and is intended only for the use of the individual and/or entity identified in the alias address of this message. If the reader of this message is not the intended recipient, or an employee or agent responsible to deliver it to the intended recipient, you are hereby requested not to distribute or copy this communication. If you have received this communication in error, please notify us immediately by telephone or return e-mail and delete the original message from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ============================================================================== The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. ============================================================================== From bwhitaker <@t> brownpathology.com Fri Dec 8 09:54:09 2006 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Fri Dec 8 09:49:59 2006 Subject: [Histonet] employment Message-ID: <002d01c71ae1$18f9b410$3601a8c0@brownpathology.net> Hi All, A histotech here in the Houston area asked me to place a post on her behalf. She is looking for employment, temporary or permanent. The Houston area would be great, but she will consider other areas. She does not want to work the night shift, however. If you have a position available, please email me off-line and I will forward the messages. Thanks, Bonnie Whitaker Lab Manager Brown & Associates Medical Laboratories 8076 El Rio Houston, Texas 77054 713-741-6677 From csn1x <@t> udcf.gla.ac.uk Fri Dec 8 10:01:50 2006 From: csn1x <@t> udcf.gla.ac.uk (Colin Nixon) Date: Fri Dec 8 10:02:04 2006 Subject: [Histonet] CD 79 antibody Message-ID: <002601c71ae2$2bd18170$d7e9d182@vet.gla.ac.uk> Does anyone know of a suitable CD79/B cell marker that works with Immunohistochemistry on formalin fixed paraffin embedded mice tissue? Colin From rjbuesa <@t> yahoo.com Fri Dec 8 10:08:25 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Dec 8 10:08:36 2006 Subject: [Histonet] CD 79 antibody In-Reply-To: <002601c71ae2$2bd18170$d7e9d182@vet.gla.ac.uk> Message-ID: <412456.26375.qm@web61221.mail.yahoo.com> I used CD79a from Novocastra (1:25 dil pH6 HIER), tonsil control. Ren? J. Colin Nixon wrote: Does anyone know of a suitable CD79/B cell marker that works with Immunohistochemistry on formalin fixed paraffin embedded mice tissue? Colin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Check out the all-new Yahoo! Mail beta - Fire up a more powerful email and get things done faster. From Richard.Breckenridge <@t> uth.tmc.edu Fri Dec 8 10:11:41 2006 From: Richard.Breckenridge <@t> uth.tmc.edu (Breckenridge, Richard A) Date: Fri Dec 8 10:11:47 2006 Subject: [Histonet] digital image analysis systems... Message-ID: Hi all, Just looking for feedback on image analysis systems that are out there. I plan on demo-ing some and any info is welcome. Thanks! Rick Richard A. Breckenridge, HT(ASCP) Technical Director/ Chief Histology Technician University of Texas- Houston Medical School Histology/ IHC Laboratory Office 713-500-6792 IHC Lab 713-500-5096 Histology Lab 713-500-5363 FAX 713-500-0733 Email - Richard.Breckenridge@uth.tmc.edu From Heather.A.Harper <@t> pcola.med.navy.mil Fri Dec 8 10:29:44 2006 From: Heather.A.Harper <@t> pcola.med.navy.mil (Harper, Heather A., CIV) Date: Fri Dec 8 10:32:03 2006 Subject: [Histonet] Bisulfite substitute for GMS Message-ID: Happy Friday everybody. Having supply issues as usual. I ran out of Sodium Bisulfite 1% for the GMS stain. Does anyone know of something to use as a substitute until I get it in? Let me know. Just used the last of it today and I'm in a pinch. Thanks in advance. Heather A. Harper Pensacola, FL Naval Hospital From thoward <@t> unm.edu Fri Dec 8 10:42:31 2006 From: thoward <@t> unm.edu (Tamara A Howard) Date: Fri Dec 8 10:42:39 2006 Subject: [Histonet] Hydrophobic barrier pens (again) Message-ID: I know this is rehashed every few months, but what is the current favorite as far as barrier/PAP pens goes? I pitched a major hissy fit last night when I was setting up an immuno run and my favorite, ancient pen finally gave up the ghost, and the "spiffy" new pen was total crap. Thanks! Tamara *************************** Tamara Howard Cell Biology & Physiology UNM-HSC Albuquerque, NM *************************** From shu-cheng.chen <@t> spcorp.com Fri Dec 8 10:56:26 2006 From: shu-cheng.chen <@t> spcorp.com (Chen, Shu-Cheng) Date: Fri Dec 8 10:57:18 2006 Subject: [Histonet] digital image analysis systems... Message-ID: <886D951F4246D94DAF28C82DC102DDD0024F1B3C@KENMSG20.us.schp.com> I am interested in this information too. Please include me in your reply. Thanks. Shu-Cheng -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breckenridge, Richard A Sent: Friday, December 08, 2006 11:12 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] digital image analysis systems... Hi all, Just looking for feedback on image analysis systems that are out there. I plan on demo-ing some and any info is welcome. Thanks! Rick Richard A. Breckenridge, HT(ASCP) Technical Director/ Chief Histology Technician University of Texas- Houston Medical School Histology/ IHC Laboratory Office 713-500-6792 IHC Lab 713-500-5096 Histology Lab 713-500-5363 FAX 713-500-0733 Email - Richard.Breckenridge@uth.tmc.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From gcallis <@t> montana.edu Fri Dec 8 11:04:56 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Dec 8 11:05:08 2006 Subject: [Histonet] ICAM-1 Antibody In-Reply-To: <001a01c71a6a$85cc4200$0bc8a8c0@your56ab121f6e> References: <001a01c71a6a$85cc4200$0bc8a8c0@your56ab121f6e> Message-ID: <6.0.0.22.1.20061208093749.01b424e0@gemini.msu.montana.edu> The KAT-1 clone should work (Serotec has this also). We would use BD Pharmingens Armenian hamster antiMouse CD54 (iCAM), negative control would be Armenian hamster IgG, Group1, kappa as the negative IgG control. BD Pharmingen has a photo available in catalog and probably on website. This antibody will bind more than just vascular tissue, it also detects lymphocytes, high endothelial venules (HEV), epithelial cells, macrophages and dendritic cells. Beware, you may NOT get staining after paraformaldehyde fixation. Read the data sheet from the website for fixation recommendations. Acetone 4C for 10 min should work, and for better morphology, acetone 75 ml / absolute ethanol 25 ml, fix overnight air dried frozen sections from fresh tissue for 5 min at room temperature, then go directly from this fixative to rinse buffer, 3 changes. At 06:45 PM 12/7/2006, you wrote: >Hi.. > >I need information about good purified rat anti mouse ICAM-1 monoclonal >antibody. I want to buy from Chemicon but there are apparently two types >of clones from Chemicon for rat antimouse ICAM-1. CLone KAT-1 and Clone >LTF653. Does anyone has any experience on using this antibody ? > >I need to stain mouse vascular tissue on frozen section with 4% >Paraformaldehyde fixation. Thanks a lot for the help. > >Regards, > >Wulan Anggrahini >Kobe University >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From rjbuesa <@t> yahoo.com Fri Dec 8 11:14:01 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Dec 8 11:14:13 2006 Subject: [Histonet] Hydrophobic barrier pens (again) In-Reply-To: Message-ID: <865945.83861.qm@web61214.mail.yahoo.com> Tamara: This may look "low tech", but I always used a "wax pencil", those you use to write on glass (either blue or red). They are cheaper and always worked fine for me. They are also "chemically inactive". Ren? J. Tamara A Howard wrote: I know this is rehashed every few months, but what is the current favorite as far as barrier/PAP pens goes? I pitched a major hissy fit last night when I was setting up an immuno run and my favorite, ancient pen finally gave up the ghost, and the "spiffy" new pen was total crap. Thanks! Tamara *************************** Tamara Howard Cell Biology & Physiology UNM-HSC Albuquerque, NM *************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From sbreeden <@t> nmda.nmsu.edu Fri Dec 8 11:16:02 2006 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Fri Dec 8 11:16:11 2006 Subject: [Histonet] Stainless Steel Pitcher Message-ID: <4D14F0FC9316DD41972D5F03C070908B0DB393@nmdamailsvr.nmda.ad.nmsu.edu> Happy Friday! I should have some subject on which to expound, but I'm too **** busy. I'm looking for a stainless steel pitcher with (insulated, preferably) handle. The use will be for transferring melted paraffin from my dispenser to my processor; it needs to fit into my oven (8" max height) and hold about, what? - a quart? I've looked in my catalogs but can't find what I'm looking for - I know they're out there... Thankseversomuch! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 From koellinr <@t> amgen.com Fri Dec 8 11:43:30 2006 From: koellinr <@t> amgen.com (Koelling, Ray) Date: Fri Dec 8 11:43:43 2006 Subject: [Histonet] GFP and B-Gal positive control slides Message-ID: <16834C6DFFA6004C88DE4507FB8AE544059AA8ED@wa-mb4-sea.amgen.com> Rebecca, The GFP antibodies we use are quirky so I don't want to make a recommendation. At Jax where you can get the Tie-2/lacZ transgenic mice, you can also get GFP expressing mice for controls. I'm not recommending, just a starting point for your search. Look under research models on their web site. For the Tie-2/lac Z mice. Fix in formalin and process to paraffin as usual. We use an antibody from Cortex Biochem (San Leandro, CA) that is IgG fraction on rabbit polyclonal to beta-galactosidase. Around 2-3 ug/ml, after a 5 min proteinase K digestion. We use an amplification kit of the third step (TSA for 5 minutes). DAB, etc. The signal is outstanding. Ray Raymond Koelling Research Scientist Amgen Seattle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rebecca Nishi Sent: Thursday, December 07, 2006 1:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GFP and B-Gal positive control slides Does anyone know where I can buy, or can suggest an easy way to make positive control slides for GFP and B-Gal. We have use AAV contstructs with either GFP or LAC-Z and injected into mice. We have sectioned the tissue and would like to look for positive GFP or B-Gal. It would be nice to have a positive control slide. We plan on visualizing both with and without antibody amplification. I would appreciate any suggestions. Thank You. Rebecca ------------------------------------ Rebecca Nishi UC Irvine Christopher Reeve Foundation SCI Core/Anderson Lab 1226 GNRF Irvine, CA 92697-4540 rnishi@uci.edu Phone/Fax: 949-824-9728 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From liz <@t> premierlab.com Fri Dec 8 11:54:41 2006 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Dec 8 11:45:16 2006 Subject: [Histonet] digital image analysis systems... In-Reply-To: Message-ID: <003701c71af1$ef9ef100$0d00a8c0@domain.Premier> We use both automeasure from Zeiss and Image Pro Plus from Mediacybernetics. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breckenridge, Richard A Sent: Friday, December 08, 2006 9:12 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] digital image analysis systems... Hi all, Just looking for feedback on image analysis systems that are out there. I plan on demo-ing some and any info is welcome. Thanks! Rick Richard A. Breckenridge, HT(ASCP) Technical Director/ Chief Histology Technician University of Texas- Houston Medical School Histology/ IHC Laboratory Office 713-500-6792 IHC Lab 713-500-5096 Histology Lab 713-500-5363 FAX 713-500-0733 Email - Richard.Breckenridge@uth.tmc.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1911 (20061208) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From gcallis <@t> montana.edu Fri Dec 8 11:59:35 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Dec 8 11:59:51 2006 Subject: [Histonet] GFP and B-Gal positive control slides In-Reply-To: <16834C6DFFA6004C88DE4507FB8AE544059AA8ED@wa-mb4-sea.amgen. com> References: <16834C6DFFA6004C88DE4507FB8AE544059AA8ED@wa-mb4-sea.amgen.com> Message-ID: <6.0.0.22.1.20061208105725.01b42518@gemini.msu.montana.edu> Ray, A question: How effective is your antibody without having to use TSA amplification? >Rebecca, > >The GFP antibodies we use are quirky so I don't want to make a >recommendation. At Jax where you can get the Tie-2/lacZ transgenic mice, >you can also get GFP expressing mice for controls. I'm not recommending, >just a starting point for your search. Look under research models on their >web site. > >For the Tie-2/lac Z mice. Fix in formalin and process to paraffin as usual. >We use an antibody from Cortex Biochem (San Leandro, CA) that is IgG >fraction on rabbit polyclonal to beta-galactosidase. Around 2-3 ug/ml, >after a 5 min proteinase K digestion. We use an amplification kit of the >third step (TSA for 5 minutes). DAB, etc. The signal is outstanding. > >Ray > >Raymond Koelling >Research Scientist >Amgen Seattle > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rebecca >Nishi >Sent: Thursday, December 07, 2006 1:34 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] GFP and B-Gal positive control slides > >Does anyone know where I can buy, or can suggest an easy way to make >positive control slides for GFP and B-Gal. > >We have use AAV contstructs with either GFP or LAC-Z and injected >into mice. We have sectioned the tissue and would like to look for >positive GFP or B-Gal. It would be nice to have a positive control >slide. We plan on visualizing both with and without antibody >amplification. I would appreciate any suggestions. > >Thank You. >Rebecca >------------------------------------ >Rebecca Nishi >UC Irvine >Christopher Reeve Foundation SCI Core/Anderson Lab >1226 GNRF >Irvine, CA 92697-4540 >rnishi@uci.edu >Phone/Fax: 949-824-9728 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From koellinr <@t> amgen.com Fri Dec 8 12:15:04 2006 From: koellinr <@t> amgen.com (Koelling, Ray) Date: Fri Dec 8 12:15:21 2006 Subject: [Histonet] GFP and B-Gal positive control slides Message-ID: <16834C6DFFA6004C88DE4507FB8AE544059AA9A5@wa-mb4-sea.amgen.com> Hi Gayle, Not very if there is minimal target. With that endothelium being so very, very thin where that particular cassette expresses, you can see it, barely, without TSA. With TSA is fantastic. If wherever your b-gal is being expressed in your particular model, if it is a nice big, plump cell you don't need amplification. But if your promoter and particular experiment means there is just not a lot of b-gal there in first place, need the TSA. For your particular experiment, if robust target there, amplification not needed. If cell type and or method of delivery makes target less robust, the TSA works wonders. Ray -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: Friday, December 08, 2006 10:00 AM To: Koelling, Ray; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] GFP and B-Gal positive control slides Ray, A question: How effective is your antibody without having to use TSA amplification? >Rebecca, > >The GFP antibodies we use are quirky so I don't want to make a >recommendation. At Jax where you can get the Tie-2/lacZ transgenic mice, >you can also get GFP expressing mice for controls. I'm not recommending, >just a starting point for your search. Look under research models on their >web site. > >For the Tie-2/lac Z mice. Fix in formalin and process to paraffin as usual. >We use an antibody from Cortex Biochem (San Leandro, CA) that is IgG >fraction on rabbit polyclonal to beta-galactosidase. Around 2-3 ug/ml, >after a 5 min proteinase K digestion. We use an amplification kit of the >third step (TSA for 5 minutes). DAB, etc. The signal is outstanding. > >Ray > >Raymond Koelling >Research Scientist >Amgen Seattle > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rebecca >Nishi >Sent: Thursday, December 07, 2006 1:34 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] GFP and B-Gal positive control slides > >Does anyone know where I can buy, or can suggest an easy way to make >positive control slides for GFP and B-Gal. > >We have use AAV contstructs with either GFP or LAC-Z and injected >into mice. We have sectioned the tissue and would like to look for >positive GFP or B-Gal. It would be nice to have a positive control >slide. We plan on visualizing both with and without antibody >amplification. I would appreciate any suggestions. > >Thank You. >Rebecca >------------------------------------ >Rebecca Nishi >UC Irvine >Christopher Reeve Foundation SCI Core/Anderson Lab >1226 GNRF >Irvine, CA 92697-4540 >rnishi@uci.edu >Phone/Fax: 949-824-9728 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From ryakay <@t> shands.ufl.edu Fri Dec 8 12:54:51 2006 From: ryakay <@t> shands.ufl.edu (Kaye Ryan) Date: Fri Dec 8 12:55:40 2006 Subject: [Histonet] ASCP review booklet Message-ID: Hi Everyone, I hope I am not going to get in trouble for this posting but not sure what else to do. I presented an ASCP review session at the past NSH meeting and I had a list of people that wanted my review booklet and that list seems to have disappeared. If you are one that was on the list could you please respond to me personally with your address and I will get it sent to you. Please do not tie up the histonet with your address info. Thanks so much, Kaye Ryan Kaye Ryan Histology Manager/Educational Coordinator Rocky Point Laboratories 265-0111, 72093 From AGrobe2555 <@t> aol.com Fri Dec 8 13:06:51 2006 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Fri Dec 8 13:07:09 2006 Subject: [Histonet] Stainless Steel Pitchers Message-ID: <233.506fdcd1.32ab11cb@aol.com> We bought ours from either Fisher or VWR. Albert From dcrippen <@t> buckinstitute.org Fri Dec 8 13:12:44 2006 From: dcrippen <@t> buckinstitute.org (Danielle Crippen) Date: Fri Dec 8 13:13:02 2006 Subject: [Histonet] mouse heart valves Message-ID: Dear All, I need to process immersion fixed (NBF) mouse heart for optimum morphological detail of the valves. Can anyone advise on the best approach?? Is FFPE or cryoprotection/freezing more recommended? Also, is 24h immersion fixation sufficient or does it need to go longer/shorter?? Any advice will be greatly appreciated!! Many thanks! d Danielle Crippen From liz <@t> premierlab.com Fri Dec 8 13:35:05 2006 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Dec 8 13:25:44 2006 Subject: [Histonet] mouse heart valves In-Reply-To: Message-ID: <000301c71aff$f64ea3c0$0d00a8c0@domain.Premier> Danielle Are you talking about mouse aortic root sections from animal models of atherosclerosis? If that's the case then depending upon how you are going to analyze the specimens you can process to paraffin or cut frozen sections. We snap freeze the heart and then cut frozen sections, since we want to be able to stain for fat with Oil Red O, but there are publications that perform paraffin sections but their image analysis of the root sections is different than the Oil Red O frozen specimens. We do this quite a bit so you can contact me and I'll go over the instructions on how to section to the correct area and how many sections we take and how we handle them, etc, it can be quite technical and labor intensive initially when working with these samples, but once you get the hang of it, its much better. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Danielle Crippen Sent: Friday, December 08, 2006 12:13 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] mouse heart valves Dear All, I need to process immersion fixed (NBF) mouse heart for optimum morphological detail of the valves. Can anyone advise on the best approach?? Is FFPE or cryoprotection/freezing more recommended? Also, is 24h immersion fixation sufficient or does it need to go longer/shorter?? Any advice will be greatly appreciated!! Many thanks! d Danielle Crippen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1911 (20061208) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From debbiekeith <@t> cox.net Fri Dec 8 13:29:35 2006 From: debbiekeith <@t> cox.net (Debbie Keith) Date: Fri Dec 8 13:29:44 2006 Subject: [Histonet] Leica cm1510 repair/follow-up Message-ID: <5.2.0.9.0.20061208121921.03044388@pop.central.cox.net> I want to thank everyone that responded to my query regarding parts for my cm1510. I called Belair Instruments and spoke to Barry (technician). not ONLY is he over-nighting me the parts... he is doing it free of charge. he spent a significant amount of time with me troublshooting. he truly went above AND beyond. I would also like to say that my previous posts shouldn't reflect poorly on the instrument. it is a fine machine. this is a clear case of operator error. (i wasn't the operator... i merely FOLLOWED the operator and sobbed uncontrollably INTO the cryo-chamber!). i would also like to thank Don (repair technician) from Leica who gave me a good idea for a "bandaid fix" until i got the part. i'm off to the hardware store to buy some non-hardening gasket sealant. (apparently CLEANING the threaded part was a bad thing. it just goes against my histo-ocd nature to NOT clean something that isn't working!) i learned today. :) histonette is a great resource! on that note... does ANYONE have a used shandon GLX linistain... cheap!? happy holidays! deb -- No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.1.409 / Virus Database: 268.15.15/580 - Release Date: 12/8/2006 From PMonfils <@t> Lifespan.org Fri Dec 8 13:46:22 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Dec 8 13:46:33 2006 Subject: [Histonet] Bisulfite substitute for GMS In-Reply-To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E273BC8@LSRIEXCH1.lsmaster.lifespan.org> Potassium bisulfite would work just as well, if you have it. Generally speaking, you can substitute a potassium salt for the equivalent sodium salt in most techniques. From Eric <@t> ategra.com Fri Dec 8 13:19:18 2006 From: Eric <@t> ategra.com (Eric Dye (ext 223)) Date: Fri Dec 8 13:48:54 2006 Subject: [Histonet] Urgent-Permanent Histology Technicians needed in Texas- should I call you to discuss ? Message-ID: Hi - Histonetters - I need Full time permanent Histology Technicians ASAP for Several Locations in Texas. This HistoTech openings are Dayshift Monday thru Friday. Full benefits and great pay ! If you are interested - call me ASAP - this is urgent !! Should I call you to discuss at ?? Or would your rather call me ? The areas in texas where the jobs are located are: Dallas area El Paso area Austin area San Angelo area Waco area Position will fill quickly. So if you are interested call me ASAP at 800-466-9919 ext 223 or cell - 407-756-5507. Also please feel free to pass my info along to others whom may be interested. Thank you, Eric Dye-Sr Allied Healthcare Recruiter 800-466-9919 ext 223 or Cell - 407-756-5507 P.S.: Feel free to pass along this email and my phone number to anyone who you think might be interested. P.S.S.: I have temp and perm histoTech jobs in other areas - if you are interested in other areas please call me as soon as possible. --------------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing 800 466 9919 ext 223 - voice 407 671 6075 - fax eric@ategra.com To Learn More About Ategra: http://www.ategra.com ---------------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. ---------------------------------------------------------------- ---------------------------------------------------------------- Note: this message is intended for: Histonetters at histonet@lists.utsouthwestern.edu ---------------------------------------------------------------- From sv18 <@t> columbia.edu Fri Dec 8 14:01:18 2006 From: sv18 <@t> columbia.edu (Sunilda Valladares-Silva) Date: Fri Dec 8 14:01:29 2006 Subject: [Histonet] Floaters Message-ID: <4579C48E.9050207@columbia.edu> Since we are on the topic of paraffin...I have a questions. Does anyone have experience with floaters on routine slides? Floaters from the paraffin as well as other specimens? From histology <@t> gradymem.org Fri Dec 8 14:33:55 2006 From: histology <@t> gradymem.org (histology@gradymem.org) Date: Fri Dec 8 14:34:04 2006 Subject: [Histonet] Stainless Steel Pitcher In-Reply-To: <4D14F0FC9316DD41972D5F03C070908B0DB393@nmdamailsvr.nmda.ad.nmsu.edu> References: <4D14F0FC9316DD41972D5F03C070908B0DB393@nmdamailsvr.nmda.ad.nmsu.edu> Message-ID: I just saw such a paraffin pitcher last night at the local Michael's craft supply store with the candle making supplies. Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org ----- Original Message ----- From: "Breeden, Sara" Date: Friday, December 8, 2006 11:29 am Subject: [Histonet] Stainless Steel Pitcher To: histonet@lists.utsouthwestern.edu > Happy Friday! I should have some subject on which to expound, but I'm > too **** busy. I'm looking for a stainless steel pitcher with > (insulated, preferably) handle. The use will be for transferring > meltedparaffin from my dispenser to my processor; it needs to fit > into my oven > (8" max height) and hold about, what? - a quart? I've looked in my > catalogs but can't find what I'm looking for - I know they're out > there... > > > > Thankseversomuch! > > > > Sally Breeden, HT(ASCP) > > NM Dept. of Agriculture > > Veterinary Diagnostic Services > > PO Box 700 > > Albuquerque, NM 87106 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From rjbuesa <@t> yahoo.com Fri Dec 8 14:52:47 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Dec 8 14:52:57 2006 Subject: [Histonet] Floaters In-Reply-To: <4579C48E.9050207@columbia.edu> Message-ID: <267711.97930.qm@web61217.mail.yahoo.com> Sunilda: If you go Histonet archieves you will find that this topic has been discussed several times in the past. Nevertheless, here are some "facts": 1-as a general rule a floater (i.e. a piece of tissue different to the processed specimen that "should not be there"), is produced during cassetting. Either because the forceps are dirty/contaminated with small pieces of "foreign" tissue or because the cassette opens in the container where small pieces of other tissue are present. Cleanliness and care are the solution, attention to detail. You can know comes from the block if the floater in the section is also present in the block. Floaters on the block USUALLY are "created" during embedding, when contaminated forceps are used or when the hot wells for them are not clean enough. Solution: clean your forcets, or even better, burn the paraffin in their tips with one electric heater (those used in microbiology to sterilize inncoulating loops). Clean the well thoroughly after finishing embedding (use a Q-tip and a plastic dropper to suck all the remnants in the well). 2-a floater in the stained slideand not in the block comes from the water bath where some fragment of a previous section was not removed from the water bath. The solution is to pass a Kimwipe over the water bath surface to catch any remnant from the just cut block from the it and prevent being "fished" with the next section. Again, care and attention to detail will eliminate this problem. Hope this will help you! Ah, another thing, floaters have happened to all HTs (no matter what they can tell you; it is a case of "being there, done that!") Ren? J. Sunilda Valladares-Silva wrote: Since we are on the topic of paraffin...I have a questions. Does anyone have experience with floaters on routine slides? Floaters from the paraffin as well as other specimens? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Access over 1 million songs - Yahoo! Music Unlimited. From LINDA.MARGRAF <@t> childrens.com Fri Dec 8 14:57:57 2006 From: LINDA.MARGRAF <@t> childrens.com (LINDA MARGRAF) Date: Fri Dec 8 14:58:13 2006 Subject: [Histonet] mouse antibodies Message-ID: <45797D75020000DA000017C7@CNET3.CHILDRENS.COM> Dear Histonetters: My colleagues are are looking for a good supplier of anti-mouse antibodies for immunohistochemistry. They already have anti-CD3 and anti-CD19, but need anti-CD4, anti-CD8, anti-macrophage/monocyte, anti-plasma cells, and anti-eosinophils. These are for studies in formalin-fixed paraffin sections of mouse tissues. Any suggestions? Thanks so much, Linda M Histonet administrator From ploykasek <@t> phenopath.com Fri Dec 8 15:22:45 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri Dec 8 15:22:58 2006 Subject: [Histonet] Slides Message-ID: Hi all. Here's a question to end the week. Has anyone experienced any problems with Surgipath "extra"(adhesive) slides? For example, getting non-"extra" slides in boxes marked "extra" slides? Or a mix of "extra"and non-"extra" in the same box?? Or are we the only lucky ones? I won't even tell you how many new grey hairs I have. On that note, I'd like some feedback on good quality adhesive (positively charged/silane) slides. Thanks for the help. TGIF. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From dfinkelstein <@t> mhri.edu.au Fri Dec 8 23:52:48 2006 From: dfinkelstein <@t> mhri.edu.au (David Finkelstein) Date: Fri Dec 8 23:53:36 2006 Subject: [Histonet] RE: digital image analysis systems... Message-ID: <000701c71b56$41d676a0$0600a8c0@davidfink> Dear Richard and Chen It depends on what type of analysis you need to do. I do not think there is one package that does all. The commercial packages can be very expensive and usually don't live up to expectation. One of the best is free from NIH!! http://rsb.info.nih.gov/ij/ If you let me know your specific need I can be a bit more specific. David Finkelstein (PhD), The Mental Health Research Institute of Victoria, dfinkelstein@mhri.edu.au Date: Fri, 8 Dec 2006 11:56:26 -0500 From: "Chen, Shu-Cheng" Subject: RE: [Histonet] digital image analysis systems... To: Cc: "Breckenridge, Richard A" Message-ID: <886D951F4246D94DAF28C82DC102DDD0024F1B3C@KENMSG20.us.schp.com> Content-Type: text/plain; charset="us-ascii" I am interested in this information too. Please include me in your reply. Thanks. Shu-Cheng -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breckenridge, Richard A Sent: Friday, December 08, 2006 11:12 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] digital image analysis systems... Hi all, Just looking for feedback on image analysis systems that are out there. I plan on demo-ing some and any info is welcome. Thanks! Rick Richard A. Breckenridge, HT(ASCP) Technical Director/ Chief Histology Technician University of Texas- Houston Medical School Histology/ IHC Laboratory Office 713-500-6792 IHC Lab 713-500-5096 Histology Lab 713-500-5363 FAX 713-500-0733 Email - Richard.Breckenridge@uth.tmc.edu From jpastor1 <@t> nycap.rr.com Sat Dec 9 11:40:16 2006 From: jpastor1 <@t> nycap.rr.com (joseph pastore) Date: Sat Dec 9 11:32:39 2006 Subject: [Histonet] Lab Vision Detection System Message-ID: <00fb01c71bb9$1713ecf0$7864a8c0@JGPONLINE> Has anyone had experience using the Lab Vision detection system. Am in the process of acquiering a IHC stainer on a reagent rental basis and its come down to either the Biocare Nemesis or the Lab Vision stainer. (Both are exactly the same. ) I am currently using the Biocare Mach 4 detection system and biocare and Dako antibodies, both of which I'm very happy with. I have had no experience with Lab Vision products and would hate to have to change my protocols to fit their products if they're inferior to Biocares. I'm at a VA hospital and am forced to go with GSA contract vendors. If I get a stainer now it would have to be Lab Vision as they have a GSA contract. I could also wait until Biocare gets a GSA which is what I'm thinking right now. Any comments on this???? From RSRICHMOND <@t> aol.com Sat Dec 9 13:40:02 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Sat Dec 9 13:40:24 2006 Subject: [Histonet] Re: Floaters Message-ID: Geezer time again. Floaters, like, uh, compost, happen. A pathologist or P.A. grossing too fast is apt to carry over fragments into the next case. If I were allowed to change the grossing ritual, I'd suggest using multiple sets of instruments (scalpels, tweezers, rulers etc) with the instruments dropped in a pot of water between specimens, and briefly washed as they accumulate. Floaters rarely cause diagnostic confusion or errors, though I've seen cases where they did cause some trouble. The worst floater accident I ever saw occurred when a paper packet containing sediment from an ascitic fluid came open and spilled a papillary ovarian carcinoma into - I counted - 26 different patients' specimens, none of which however would have been likely to cause a diagnostic error. (The patient from whom the ascitic fluid came was not strongly suspected of having cancer.) Large papillary urothelial (worst of all renal pelvis) tumors are perhaps most likely to fragment, and should be grossed with very great care. A pathologist should NEVER mention a floater in a report. You take one of those glass-marking pens the ThinPrep folks give you, circle the offending object on the slide, and write "floater" beside the circle. I don't usually call them to the attention of the responsible histotechnologist, though perhaps I should. Bob Richmond Samurai Pathologist Knoxville TN From arvidsonkristen <@t> yahoo.com Sun Dec 10 07:35:34 2006 From: arvidsonkristen <@t> yahoo.com (kristen arvidson) Date: Sun Dec 10 07:35:44 2006 Subject: [Histonet] New rapid processors Message-ID: <938549.47174.qm@web61313.mail.yahoo.com> Hello! We are in the process of budgeting for new processors. The idea of these new (large) rapid processors has come up (I think they are considered microwave processors). Our current problem is volume not turn-around times, because in our lab turn-around times are only as good as the Pathologist reading the case. I guess my question is what is the benefit to these (besides turn-around), because they are very expensive. How do they work? Can you put all types of tissue in there? I feel with our needs a more conventional processor would be better (better for the budget!). Also, they are fairly new...and we have had brand new equipment in our lab before and it seems the bugs were never quite worked out. Help!! -Kristen __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From modernarnis73 <@t> juno.com Sun Dec 10 07:59:20 2006 From: modernarnis73 <@t> juno.com (modernarnis73@juno.com) Date: Sun Dec 10 08:00:48 2006 Subject: [Histonet] Mi Society of Histotechnologists Website? Message-ID: <20061210.055939.26645.1651468@webmail39.lax.untd.com> Hello Everyone, Does anyone know what happened to the Michigan Society of Histotechnologists Website? It's no longer up www.Mshonline.org Did they happen to change their address or is it down due to maintance? Thanks in advance & Happy Holidays!!! Enoch ________________________________________________________________________ Try Juno Platinum for Free! Then, only $9.95/month! Unlimited Internet Access with 1GB of Email Storage. Visit http://www.juno.com/value to sign up today! From rjbuesa <@t> yahoo.com Sun Dec 10 08:38:47 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Sun Dec 10 08:38:55 2006 Subject: [Histonet] New rapid processors In-Reply-To: <938549.47174.qm@web61313.mail.yahoo.com> Message-ID: <779843.50460.qm@web61215.mail.yahoo.com> Kristen: I have just finished an article about "Microwave assisted tissue processing" and I am going to send it to you under separate cover, but for the benefit of all (as Histonet is intended to be) I am also answering now. 1- Mirowave assisted tissue processing (MWATP) is a way of increasing the turn around time (TAT) only because the tissue processing (TP) step is reduced. It can be used in all types of tissues with results qualitativaly comparable to those obtained with conventional tissue processing. 2-Having said that, their effect stops there because they CANNOT affect the rest of the workflow. 3-the histology workflow is composed of 11 fundamental tasks, and TP is just one of them that, depending on the technology, represents 3 to 34% of the total time, the rest of the time (66 to 97%) has to be dedicated to the other tasks 4- REGARDLESS of the technology there will be needed 16.42hours to prepare 100 routine slides from as many cassettes, divided into 7.9 h of pre-TP tasks and 8.52 h of post-TP activities. 5- If you process 210 cassettes OVERNIGHT with a conventional tissue processor, or in just 1 hour with the Pathos or in 2 hours with the Xpress, in EACH case you will have to spend ANOTHER 21.8 h of work to complete the slides (7.4 h pre-TP and 13.4h post-TP) and this CANNOT be avoided. 6- from a flow of work point of view and aiming at a "Lean oriented" process, you should spend about the same time in pre-TP tasks than during processing, with a total of post-TP time similar to pre-TP + processing times combined. The least amount of cassettes you can process with present technology that conforms to that scheme are 15 cassettes processed in 0.42h, after 0.53h of pre-TP tasks and a total of 1.05h of post-TP tasks for a general flow time of 2 hours. 7- If your concern is NOT a reduction in TAT you are better off by using a conventional tissue processor twice a day with a short protocol (1.5 to 2 hours) to take care of the small biopsies and have for them a daily TAT, and the rest of the cases processed overnight with a "conventional" 6 to 8 h protocol. 8- you could also buy a small MWATP to prepare the small biopsies and the rest of the cases overnight witha conventional tissue processor. I hope this notes (and the article I am going to send you) will help you! Ren? J. kristen arvidson wrote: Hello! We are in the process of budgeting for new processors. The idea of these new (large) rapid processors has come up (I think they are considered microwave processors). Our current problem is volume not turn-around times, because in our lab turn-around times are only as good as the Pathologist reading the case. I guess my question is what is the benefit to these (besides turn-around), because they are very expensive. How do they work? Can you put all types of tissue in there? I feel with our needs a more conventional processor would be better (better for the budget!). Also, they are fairly new...and we have had brand new equipment in our lab before and it seems the bugs were never quite worked out. Help!! -Kristen __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Everyone is raving about the all-new Yahoo! Mail beta. From barry_m <@t> ozemail.com.au Sun Dec 10 16:40:28 2006 From: barry_m <@t> ozemail.com.au (Barry Madigan) Date: Sun Dec 10 16:40:52 2006 Subject: [Histonet] Slides In-Reply-To: Message-ID: <000001c71cac$321cf660$0201010a@WORKSTATION1> We have no major problems with lifting of tissue on the BondMax ImmunoStainer using SuperFrost Plus slides except for nerve tissue. We perform a number of CD markers on nerve tissue and they all require heat retrieval. There just doesn't seem to be enough adhesion with these specimens. I was wondering if anyone else has experienced a similar experience and worked out a solution. Regards Barry Immunohistochemistry QHPS-Central Brisbane Australia -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Patti Loykasek Sent: Saturday, 9 December 2006 7:23 AM To: histonet Subject: [Histonet] Slides Hi all. Here's a question to end the week. Has anyone experienced any problems with Surgipath "extra"(adhesive) slides? For example, getting non-"extra" slides in boxes marked "extra" slides? Or a mix of "extra"and non-"extra" in the same box?? Or are we the only lucky ones? I won't even tell you how many new grey hairs I have. On that note, I'd like some feedback on good quality adhesive (positively charged/silane) slides. Thanks for the help. TGIF. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Asf2k3 <@t> aol.com Sun Dec 10 22:50:59 2006 From: Asf2k3 <@t> aol.com (Asf2k3@aol.com) Date: Sun Dec 10 22:51:15 2006 Subject: [Histonet] Histotech Jobs in Atlanta? Message-ID: Does any one know if there are any full-time openings jobs for histotechs in Atlanta? I am looking for one. Clinics? maybe... A.S From wwzhang <@t> gmail.com Mon Dec 11 00:40:34 2006 From: wwzhang <@t> gmail.com (Wen Zhang) Date: Mon Dec 11 00:40:46 2006 Subject: [Histonet] Re: need a protocol for whole rat brain tissue In-Reply-To: <033001c71306$c5989070$ec04a8c0@sistemas> References: <033001c71306$c5989070$ec04a8c0@sistemas> Message-ID: <20061211.144034.115906020.wwzhang@gmail.com> Hi, I'm also interested in your paper Biocell (1996. 20(3): 265-272). Can you send a PDF of it? From Kemlo.Rogerson <@t> waht.swest.nhs.uk Mon Dec 11 03:00:54 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Dec 11 02:59:48 2006 Subject: [Histonet] Re: Floaters Message-ID: <86ADE4EB583CE64799A9924684A0FBBF01246F89@wahtntex2.waht.swest.nhs.uk> With respect compost just does happen; it is a very delicate process incorporating bacteria and aerated vegetable material, usually. Too wet and you get a sick fermentation, too dry and you don't get any. I knew this Pathologist who spent ages rotarising vegetable matter at god knows what time in the morning to produce compost to grow marrows on the side of a mine spillage mound. What was his name.......? I don't know how you can ignore floaters unless you revisit the block and investigate its presence or absence. That amount of tumour seedling is worrying, do you not filter the alcohols regularly and change the last batch? If you accept their presence then sods law will place them in a place on a slide that you accept to present reality and surely it's no defense just to say that it is a floater; that is just so much like the other type of floater, with respect. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Once you are in the orbit of your destiny, weightlessness is the only result. --Baba Amte This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Kemlo.Rogerson <@t> waht.swest.nhs.uk Mon Dec 11 03:10:56 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Mon Dec 11 03:09:54 2006 Subject: [Histonet] Floaters Message-ID: <86ADE4EB583CE64799A9924684A0FBBF01246F8A@wahtntex2.waht.swest.nhs.uk> We used to suffer from floaters when I was a pup; you have to be careful with certain friable tumours (ovarian is certainly a good example) and filter solvents regularly; this includes paraffin which sometimes has a crud of indescribable things (just block it out and frighten yourself). My old Senior Chief (dead now god rests his soul) used to have pitchers of paraffin filtering in the oven; but poor old Colin was well before his time and discovered pollution before any one else. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Once you are in the orbit of your destiny, weightlessness is the only result. --Baba Amte This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From Jeannette.Mitchell <@t> vtmednet.org Mon Dec 11 05:43:37 2006 From: Jeannette.Mitchell <@t> vtmednet.org (Mitchell, Jeannette M.) Date: Mon Dec 11 05:43:44 2006 Subject: [Histonet] Hercept Message-ID: <8C7B2CE85A8CBA49B3FE05DE478412ED0169D732@FAHC14.fahc.fletcherallen.org> What is everyone using for their Hercept antibody? I realize that Dako has a kit that is FDA approved. If you are not using FDA approved antibody(kit) are you charging the patient for the testing? Are the patients allowed to received Herceptin even if the antibody is not FDA approved? Any insight on what people are using out there is much appreciated. Thanks again, Jeannette Mitchell, BS, HTL(ASCP), QIHC R&D Histotechnican EP1-119 Fletcher Allen Health Care Burlington, VT 05403 Phone # 802-847-3096 & 802-847-5362 Confidentiality Notice: This message, and any attachments, may contain information that is confidential, privileged, and/or protected from disclosure under state and federal laws that deal with the privacy and security of medical information. If you received this message in error or through inappropriate means, please reply to this message to notify the Sender that the message was received by you in error, and then permanently delete this message from all storage media, without forwarding or retaining a copy. From nancy.troiano <@t> yale.edu Mon Dec 11 07:04:46 2006 From: nancy.troiano <@t> yale.edu (Nancy W. Troiano) Date: Mon Dec 11 07:04:54 2006 Subject: [Histonet] state website problems Message-ID: <5.2.1.1.2.20061211075626.00c3e008@email.med.yale.edu> In response to the loss of the Michigan Histotechnology website problems -I'd to let you know that our Connecticut State website was suddenly eliminated (it was a free website sponsored by LabVision) and when we inquired at LabVision as to why our website was suddenly eliminated and reverted to godaddy.com (when it wasn't set to expire until Dec 2007) it turns out that LabVision had no idea what we were talking about as far as website sponsorship (even the marketing dept had no clue what we were talking about). It turns out that the website sponsoring was through Jim Kieffer of FX Roads (a consultant for LabVision) and both Jim Kieffer and FX Roads have disappeared from the face of the earth so our attempts to contact him or his company have been unsuccessful. Now all of our hard work in setting up the website is lost, and we can't even take over possession of the cthisto.org website without finding Jim Kieffer or his company and getting their approval. It is a nightmare to say the least - and a warning that nothing for free is worth it. To those state societies using LabVision as a sponsor of their websites - beware....this could happen to you too. Can anybody give me information on how to establish a new website (that our own State society will pay for)? I don't know how to go about doing this. Thanks - Nancy Troiano (203)785-5136 From kmerriam2003 <@t> yahoo.com Mon Dec 11 07:46:48 2006 From: kmerriam2003 <@t> yahoo.com (Kim Merriam) Date: Mon Dec 11 07:47:00 2006 Subject: [Histonet] mouse antibodies Message-ID: <20061211134648.12417.qmail@web50303.mail.yahoo.com> Linda, Serotec and BD Pharmingen have a lot of anti-mouse antibodies. These 2 vendors would be a good place to start. Good luck, Kim Kim Merriam, MA, HT(ASCP) Amgen Cambridge, MA ----- Original Message ---- From: LINDA MARGRAF To: histonet@lists.utsouthwestern.edu Cc: ANA GOMEZ Sent: Friday, December 8, 2006 3:57:57 PM Subject: [Histonet] mouse antibodies Dear Histonetters: My colleagues are are looking for a good supplier of anti-mouse antibodies for immunohistochemistry. They already have anti-CD3 and anti-CD19, but need anti-CD4, anti-CD8, anti-macrophage/monocyte, anti-plasma cells, and anti-eosinophils. These are for studies in formalin-fixed paraffin sections of mouse tissues. Any suggestions? Thanks so much, Linda M Histonet administrator _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________ Need a quick answer? Get one in minutes from people who know. Ask your question on www.Answers.yahoo.com From godsgalnow <@t> aol.com Mon Dec 11 08:55:23 2006 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Mon Dec 11 08:55:40 2006 Subject: [Histonet] anyone want to buy used equipment Message-ID: <8C8EB4B850F53E4-4EC-3AA4@FWM-D27.sysops.aol.com> We have several pieces of equipment that we want to sell. ANyone interest? This is the list we have... 2-3 Olympus micrtomes 4 ATP1 processors (TBS) 3 - 4 embedding centers (TBS) If anyone is interested please let me know..... Roxanne Soto HT(ASCP)QIHC Lab Manager Physicians RightPath Tampa, Florida 813-549-1050 ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From s.tripathy <@t> student.qut.edu.au Tue Dec 5 19:36:45 2006 From: s.tripathy <@t> student.qut.edu.au (Srikant Tripathy) Date: Mon Dec 11 09:59:58 2006 Subject: [Histonet] Cryosectioning of retina/choroid/sclera? Message-ID: <001101c718d6$fd14df60$baacb583@ihbi.qut.edu.au> Hi, I am Srikant a PhD student in Queensland University of Technology,Brisbane,Australia.In a part of my study I am trying to estimate the element concentration (Na,K,Cl) at various layers of eye (retina,choroid,sclera) by EDX analysis and confocal microscope. I am a Pharmacist and don't have very good knowledge of histological techniques. I am using cryosectioning of eye layers and for a fragile layer like retina I need to cryoprotect before sectioning. I am using snap freezing technology. My doubts are: 1. As I can't use water soluble cryoprotectant as this will leach all soluble ion. I wonder how people can keep retina soaked in Ames's solution and gets fluorescence for chloride ions using dyes like MEQ. 2. Can any one please guide me how I will proceed for cryoprotecting and sectioning the retina/choroid/sclera with out adverse effects on elemental concentration? 3. Can I use PEG as a coating material prior to cryosectioning for elemental analysis before EDX and confocal microscopy? Thanks. Srikant Srikant Tripathy | PhD Student | Institute of Health and Biomedical Innovation Queensland University of Technology | Corner of Musk Avenue and Blamey Street, Kelvin Grove QLD 4059 Australia t: +61 (0) 7 3138 6157 | f: +61 (0) 7 3138 6030 | m: 0423586276 | e: s.tripathy@qut.edu.au | w: www.ihbi.qut.edu.au CRICOS No. 00213J From tim.morken <@t> thermofisher.com Wed Dec 6 10:38:40 2006 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Mon Dec 11 09:59:59 2006 Subject: ASR disclaimer and RUO's, was...RE: [Histonet] FITC C4d In-Reply-To: <457691380200007700003213@hcnwgwds01.hh.chs> Message-ID: Yes, the disclaimer is only for ASR's. There is no disclaimer for RUO antibodies because RUO's are not to be used for diagnostics, at least they are not to be reported or charged. This is an FDA rule and they say the CAP is wrong to say a diagnostic lab can use RUO's (CAP has a section in the checklist that says an RUO can be used if the lab cannot find the antibody as an ASR or IVD. FDA says this is not correct and no use of RUO for diagnostics is legal). However, FDA has made no effort to enforce this rule. Below is my quesiton to FDA and their answer. Tim Morken Product Development Lab Vision - Neomarkers ThermoFisher Scientific Here is my question to FDA and their answer. I asked in 2004 and confirmed this in 2006. Recently (Dec 2004, (http://www.cap.org/apps/docs/laboratory_accreditation/checklists/checkl istf tp.html) The College of American Pathologists (CAP), the accrediting agency for diagnostic Anatomic Pathology labs, determined that RUO antibody reagents are suitable for diagnostic work as long as the laboratory using them makes a "reasonable" effort to find such antibodies in IVD or ASR format, and undergoes validation of such antibodies: from: Anatomic Pathology checklist, Sept 2004, Item # ANP.12425 "Antibodies, nucleic acid sequences, etc., labeled "Research Use Only" (RUO) purchased from commercial sources may be used in home brew tests only if the laboratory has made a reasonable effort to search for IVD or ASR class reagents. The results of that failed search should be documented by the laboratory director." My questions are: 1)Does the FDA approve of this reversal of the policy to not allow RUO's for diagnostic use. 2) Is the requirement to be FDA registered now moot for antibody use (RUO-only vendors are not usually FDA registered) 3) If RUO's can be used, is there a disclaimer (as for ASR's) that must be used along with RUO reagents? -----Original Message----- From: Gutman, Steve [mailto:SIG@CDRH.FDA.GOV] Sent: Tuesday, April 05, 2005 8:14 AM To: 'tpmorken@labvision.com' Cc: St. Pierre, Don J.; Rodgers, Anthony (CDRH); Cardamone, Thomas E.; Yost, Judith A (CMS) Subject: FW: 03-393 aqr DSMICA Email Form Response Dear Mr. Morken, I was not familiar with this CAP change and will share this with CMS the organization that grants deemed status to CAP. While I do think this is a pragmatic approach and probably well intentioned, I also think unfortunately it is clearly at odds with the law. Companies or laboratories that follow this are potentially in jeopardy of compliance action. If you have questions or concerns, please feel free to call me at 240-328-0484. Steven Gutman, M.D. Director, Office of In Vitro Diagnostics -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Wednesday, December 06, 2006 6:45 AM To: Donna Lawson; histonet@lists.utsouthwestern.edu; Mark Tarango Subject: RE: [Histonet] FITC C4d I thought the disclaimer was for analyte-specific reagents (ASRs) only? Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Tarango, Mark" 12/05/06 5:55 PM >>> I've tried and didn't find one. It's all about the good faith effort, if you ask me. . . You have that FDA disclaimer on the reports afterall, right? Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Donna Lawson Sent: Tuesday, December 05, 2006 12:03 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FITC C4d HI, Is anyone out there using an indirect FITC labeled C4d antibody that is NOT an RUO? I can only find 2 and they both say for RUO not for Clinical Diagnostics. Any Help will be greatly appreciated, Donna Lawson H.T. (ASCP) QIHC Histology Supervisor University of Kansas Hospital 913-588-1134 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------ ------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ======================================================================== ====== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pkarlisch <@t> psu.edu Mon Dec 11 10:14:26 2006 From: pkarlisch <@t> psu.edu (Patricia Karlisch) Date: Mon Dec 11 10:14:52 2006 Subject: [Histonet] Hershey PA Message-ID: <457D3D920200008C00032214@GWIA02.HERSHEYMED.NET> All, We are looking for a qualified HT/HTL histotechnician to work at Penn State Hershey Medical Center in Hershey, Pennsyslvania, full time on rotating shifts. Is there anyone willing to relocate to this quiet town of Hershey (yes, where they make the chocolates). We would love to speak with you. The area is close to Harrisburg, Baltimore and Philly. The view is awesome and there are many things to do. If you are interested please email me only @ pkarlisch@ psu.edu. I would love to hear from you. Pat Karlisch Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. From histology <@t> gradymem.org Mon Dec 11 10:32:49 2006 From: histology <@t> gradymem.org (histology@gradymem.org) Date: Mon Dec 11 10:32:55 2006 Subject: [Histonet] state website problems In-Reply-To: <5.2.1.1.2.20061211075626.00c3e008@email.med.yale.edu> References: <5.2.1.1.2.20061211075626.00c3e008@email.med.yale.edu> Message-ID: When I checked on the Oklahoma website earlier today it was not there. I checked again just now and it is back up. Visit us at www.okhisto.org Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org ----- Original Message ----- From: "Nancy W. Troiano" Date: Monday, December 11, 2006 7:12 am Subject: [Histonet] state website problems To: histonet@lists.utsouthwestern.edu > In response to the loss of the Michigan Histotechnology website > problems > -I'd to let you know that our Connecticut State website was > suddenly > eliminated (it was a free website sponsored by LabVision) and when > we > inquired at LabVision as to why our website was suddenly > eliminated and > reverted to godaddy.com (when it wasn't set to expire until Dec > 2007) it > turns out that LabVision had no idea what we were talking about as > far as > website sponsorship (even the marketing dept had no clue what we > were > talking about). It turns out that the website sponsoring was > through Jim > Kieffer of FX Roads (a consultant for LabVision) and both Jim > Kieffer and > FX Roads have disappeared from the face of the earth so our > attempts to > contact him or his company have been unsuccessful. Now all of our > hard > work in setting up the website is lost, and we can't even take > over > possession of the cthisto.org website without finding Jim Kieffer > or his > company and getting their approval. It is a nightmare to say the > least - > and a warning that nothing for free is worth it. To those state > societies > using LabVision as a sponsor of their websites - beware....this > could > happen to you too. Can anybody give me information on how to > establish a > new website (that our own State society will pay for)? I don't > know how > to go about doing this. Thanks - Nancy Troiano (203)785-5136 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gcallis <@t> montana.edu Mon Dec 11 10:56:25 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Dec 11 10:56:41 2006 Subject: [Histonet] floaters Message-ID: <6.0.0.22.1.20061211094306.01d50970@gemini.msu.montana.edu> Yes, we all have had the problem at one time or another - excellent points about friable tissue. I think one reply on Histonet, the tech used newpaper to skim the waterbath surface. Make sure the paper is not overly fibrous paper, you don't need shreds of paper on top of your sections either. One of the most annoying floaters comes from the microtomist. Epidemal cells from the skin exfoliate into the waterbath especially when you allow fingers to dip into the water, and then stain very nicely with the H&E, generally perched on your beautiful section. Grip the slides on the edge or very ends to avoid this. Pencil "lead" or graphite exfoliates black blobs on tissue sections, use a slightly harder lead, buy a slide labeler, or use a permanent pen designed for labeling glass slides/cassettes. Ren? brings up a good point about dirty forceps/heated forcep wells on embedding centers. At one clinical lab, we wiped the forceps between each block before returning to the well, and cleaned the wells daily. We still saw the annoying skin cells though, so learn to not go 'fingerdipping" in the water bath. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From ree3 <@t> leicester.ac.uk Mon Dec 11 11:14:55 2006 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Mon Dec 11 11:15:07 2006 Subject: [Histonet] floaters In-Reply-To: <6.0.0.22.1.20061211094306.01d50970@gemini.msu.montana.edu> Message-ID: Often referred to as "squams", also derived from flaky scalps, i.e. dandruff!!. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis Sent: 11 December 2006 16:56 To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] floaters Yes, we all have had the problem at one time or another - excellent points about friable tissue. I think one reply on Histonet, the tech used newpaper to skim the waterbath surface. Make sure the paper is not overly fibrous paper, you don't need shreds of paper on top of your sections either. One of the most annoying floaters comes from the microtomist. Epidemal cells from the skin exfoliate into the waterbath especially when you allow fingers to dip into the water, and then stain very nicely with the H&E, generally perched on your beautiful section. Grip the slides on the edge or very ends to avoid this. Pencil "lead" or graphite exfoliates black blobs on tissue sections, use a slightly harder lead, buy a slide labeler, or use a permanent pen designed for labeling glass slides/cassettes. Ren? brings up a good point about dirty forceps/heated forcep wells on embedding centers. At one clinical lab, we wiped the forceps between each block before returning to the well, and cleaned the wells daily. We still saw the annoying skin cells though, so learn to not go 'fingerdipping" in the water bath. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tim.morken <@t> thermofisher.com Mon Dec 11 10:40:33 2006 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Mon Dec 11 11:21:19 2006 Subject: [Histonet] state website problems In-Reply-To: <5.2.1.1.2.20061211075626.00c3e008@email.med.yale.edu> Message-ID: Nancy and others with websites sponsored by Lab Vision, I'd like to reassure you that the State Society Webhosting sponsorship is still ongoing and that we have only had some technical problems with some of the websites (in fact, only 3 out of 17 sponsored website have had problems). FX Roads is still in business and I am working with them to resolve the issues. We plan to have all these websites back online this week. Tim Morken Lab Vision - Neomarkers ThermoFisher Scientific -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy W. Troiano Sent: Monday, December 11, 2006 5:05 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] state website problems In response to the loss of the Michigan Histotechnology website problems -I'd to let you know that our Connecticut State website was suddenly eliminated (it was a free website sponsored by LabVision) and when we inquired at LabVision as to why our website was suddenly eliminated and reverted to godaddy.com (when it wasn't set to expire until Dec 2007) it turns out that LabVision had no idea what we were talking about as far as website sponsorship (even the marketing dept had no clue what we were talking about). It turns out that the website sponsoring was through Jim Kieffer of FX Roads (a consultant for LabVision) and both Jim Kieffer and FX Roads have disappeared from the face of the earth so our attempts to contact him or his company have been unsuccessful. Now all of our hard work in setting up the website is lost, and we can't even take over possession of the cthisto.org website without finding Jim Kieffer or his company and getting their approval. It is a nightmare to say the least - and a warning that nothing for free is worth it. To those state societies using LabVision as a sponsor of their websites - beware....this could happen to you too. Can anybody give me information on how to establish a new website (that our own State society will pay for)? I don't know how to go about doing this. Thanks - Nancy Troiano (203)785-5136 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From osullivan <@t> mpih-frankfurt.mpg.de Mon Dec 11 11:53:19 2006 From: osullivan <@t> mpih-frankfurt.mpg.de (Gregory A. O'Sullivan) Date: Mon Dec 11 11:53:35 2006 Subject: [Histonet] Rapid freezing using n-Pentane Message-ID: <014901c71d4d$3f100410$d205058d@MPIHFrankfurt.mpg.de> Dear Histonetters, Could anyone give a reference or a protocol for the rapid freezing of postnatal P1 brains from rodents? We require such a protocol in order to minimise the amount of shearing that is observed by rapid freezing on dry-ice. Many thanks, Greg From lpwenk <@t> sbcglobal.net Mon Dec 11 12:26:42 2006 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Mon Dec 11 12:27:24 2006 Subject: [Histonet] Floaters In-Reply-To: <4579C48E.9050207@columbia.edu> Message-ID: <002101c71d51$e8c01f50$59ff2d4b@HPPav2> See if you can get a copy of Arch Pathol Lab Med 1996 Nov;120(11):1009-1014. Extraneous Tissue in Surgical Pathology: A College of American Pathologists Q-Probe Study of 275 Laboratories. This was a CAP study. I don't have the article in front of me right now, so I'll generalize. They asked pathologists to look at every slide that passed their desk for "x" number of weeks (prospective study), AND to pull and look at every slide that they had done for "y" number of weeks BEFORE they started the study (retrospective study). Any slide with floaters (extraneous tissue), they had to trace back, to see if it was on the slide only, block only, in the specimen only, etc. They also had to determine who was reponsible for submitting the extaneous tissue. And they wanted to see if there was a difference in number of floaters before and after they started their study (people might be taking more care because they knew their work was being checked). One finding was that the incidents of floaters decreased 4-5x in the prospective (current) work than the retrospective (old slides) study. So when people knew they were being monitored, they took care and had fewer floaters. Also, the people most responsible for the extraneous tissue: (Retrospective stats, rounded to nearest whole number) Pathogists = 51% Residents = 21% PA = 17% Histotec = 9% Other/unknown = 3% In the prospective study, the residents improved a lot (dropped 12%), and the other people's percents increased 4-5%, but that's because of the improvement of resident's making fewer mistakes. Also, my opinion, this was 10 years ago. How many labs no longer have pathologists doing their own grossing, but now have histotechs and PA's? So I don't know if the stats would be exactly the same today. However, I do think it points out that histotechs are not the only cause of floaters, and are probably the least likely cause the floaters. But, yes, if we are not careful, histotechs can cause floaters while embedding or sectioning. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sunilda Valladares-Silva Sent: Friday, December 08, 2006 3:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Floaters Since we are on the topic of paraffin...I have a questions. Does anyone have experience with floaters on routine slides? Floaters from the paraffin as well as other specimens? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Mon Dec 11 12:51:56 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Dec 11 12:52:16 2006 Subject: [Histonet] mouse antibodies In-Reply-To: <20061211134648.12417.qmail@web50303.mail.yahoo.com> References: <20061211134648.12417.qmail@web50303.mail.yahoo.com> Message-ID: <6.0.0.22.1.20061211115011.01b86dc0@gemini.msu.montana.edu> And the CD4 and CD8 will not work on formalin fixed paraffin sections even after retrievals or digestion. These need to be done on frozen sections from fresh snap frozen tissues. There may other CD markers mentioned that do not work on FFPE either. Sorry to be a wet blanket At 06:46 AM 12/11/2006, you wrote: >Linda, > >Serotec and BD Pharmingen have a lot of anti-mouse antibodies. These 2 >vendors would be a good place to start. > >Good luck, >Kim > >Kim Merriam, MA, HT(ASCP) >Amgen >Cambridge, MA > > > >----- Original Message ---- >From: LINDA MARGRAF >To: histonet@lists.utsouthwestern.edu >Cc: ANA GOMEZ >Sent: Friday, December 8, 2006 3:57:57 PM >Subject: [Histonet] mouse antibodies > > >Dear Histonetters: >My colleagues are are looking for a good supplier of anti-mouse >antibodies for immunohistochemistry. They already have anti-CD3 and >anti-CD19, but need anti-CD4, anti-CD8, anti-macrophage/monocyte, >anti-plasma cells, and anti-eosinophils. These are for studies in >formalin-fixed paraffin sections of mouse tissues. >Any suggestions? Thanks so much, >Linda M >Histonet administrator > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >____________________________________________________________________________________ >Need a quick answer? Get one in minutes from people who know. >Ask your question on www.Answers.yahoo.com >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From gcallis <@t> montana.edu Mon Dec 11 13:05:24 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Dec 11 13:05:47 2006 Subject: [Histonet] mouse heart valves In-Reply-To: References: Message-ID: <6.0.0.22.1.20061211115551.01b8cf30@gemini.msu.montana.edu> There was a wonderful NSH poster by Joanna Barton et al using Histogel for rat heart valve tissue sections. Rat hearts (you could also do this on mouse hearts) were quickly removed, flushed then filled with neutral buffered formlin via the aorta. All major vessels were clamped, hearts were immersed in NBF 3 to 6 hours (lesser time for mouse may be adequate due to size) . Hearts were then infused with prewarmed 52C Histogel, . They filled all chambers via aorta using 20 gauge needle, then fixed for 48 hours. Hearts were cut using heart matrices to have precise orientation and properly thick slicecs. Email Joanna at joanna.c.barton@gsk.com for more details and when you do this, ask her to publish this technic in J of Histotechnolgy. The heart sections were superb with all tiny valve morphological details intact due to the distended chambers. There were no a huge clot of blood in the chambers either. At 12:12 PM 12/8/2006, you wrote: >Dear All, > >I need to process immersion fixed (NBF) mouse heart for optimum >morphological detail of the valves. Can anyone advise on the best >approach?? Is FFPE or cryoprotection/freezing more recommended? Also, is >24h immersion fixation sufficient or does it need to go >longer/shorter?? Any advice will be greatly appreciated!! > >Many thanks! > >d > >Danielle Crippen > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From rgarhart <@t> system1.net Mon Dec 11 14:11:17 2006 From: rgarhart <@t> system1.net (Robert Garhart) Date: Mon Dec 11 14:08:24 2006 Subject: [Histonet] Lab Director with Histology Background Message-ID: Lab Director Position Available - Location: SW United States Requirements: 10+ years in a lab setting 5+ years in supervisory role HT or MT Certification Familiarity with CAP and CLIA Guidelines ASCP Certification Description: This is a Lab Director opportunity reporting to the VP of Lab Operations. This is a brand new lab and this person will play an integral role in getting the lab up and running. Any prior experience in this area is a big plus. There will be 4-6 pathologists in a very GI Pathology centric atmosphere. Any interest or questions should be directed to Robert Garhart rgarhart@system1.net or you can call 866-797-8361. Robert Garhart Executive Recruiter System 1 Search 864.627.0012 Phone 864.627.0013 Fax 866.797.8361 Toll Free rgarhart@system1.net From vazquezr <@t> ohsu.edu Mon Dec 11 14:42:36 2006 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Mon Dec 11 14:43:15 2006 Subject: [Histonet] traveling mohs tech Message-ID: Hello, We are in need of a traveling Mohs tech that is located here in Portland Oregon and on our list of approved techs to work for OHSU. To fill in on sick days and vacations. Thanks in advanced. Robyn OHSU @ Center for Health and Healing 503-494-4658 From pmarcum <@t> vet.upenn.edu Mon Dec 11 15:04:03 2006 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Mon Dec 11 15:04:13 2006 Subject: [Histonet] state website problems In-Reply-To: References: <5.2.1.1.2.20061211075626.00c3e008@email.med.yale.edu> Message-ID: <6.2.5.6.2.20061211160236.01bcaf08@vet.upenn.edu> Thank You Tim and LabVison for all the work and help on these web sites. We do appreciate it and are very glad ti will continue with the new organization. I am sure we all know how hard computer issues can bite us. Pam Marcum At 11:40 AM 12/11/2006, Morken, Tim wrote: >Nancy and others with websites sponsored by Lab Vision, I'd like to >reassure you that the State Society Webhosting sponsorship is still >ongoing and that we have only had some technical problems with some of >the websites (in fact, only 3 out of 17 sponsored website have had >problems). FX Roads is still in business and I am working with them to >resolve the issues. We plan to have all these websites back online this >week. > > >Tim Morken >Lab Vision - Neomarkers >ThermoFisher Scientific > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy W. >Troiano >Sent: Monday, December 11, 2006 5:05 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] state website problems > >In response to the loss of the Michigan Histotechnology website problems >-I'd to let you know that our Connecticut State website was suddenly >eliminated (it was a free website sponsored by LabVision) and when we >inquired at LabVision as to why our website was suddenly eliminated and >reverted to godaddy.com (when it wasn't set to expire until Dec 2007) it >turns out that LabVision had no idea what we were talking about as far >as website sponsorship (even the marketing dept had no clue what we were >talking about). It turns out that the website sponsoring was through >Jim Kieffer of FX Roads (a consultant for LabVision) and both Jim >Kieffer and FX Roads have disappeared from the face of the earth so our >attempts to contact him or his company have been unsuccessful. Now all >of our hard work in setting up the website is lost, and we can't even >take over possession of the cthisto.org website without finding Jim >Kieffer or his company and getting their approval. It is a nightmare to >say the least - and a warning that nothing for free is worth it. To >those state societies using LabVision as a sponsor of their websites - >beware....this could happen to you too. Can anybody give me information >on how to establish a >new website (that our own State society will pay for)? I don't know >how >to go about doing this. Thanks - Nancy Troiano (203)785-5136 > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From ergonomics <@t> ehs.unc.edu Mon Dec 11 15:46:47 2006 From: ergonomics <@t> ehs.unc.edu (Bertmaring, Ian (Environment Health & Safety)) Date: Mon Dec 11 15:46:56 2006 Subject: [Histonet] Grossing postures and proceedures - Ergonomics In-Reply-To: Message-ID: <79ACF88C02E12F419FE8ECA36001D8FAA3315F@auxsexc1.aux-services.unc.edu> I have a situation that I am looking for a solution for and I figured some one in this community could help me. The Problem: When techs at UNC perform grossing, they often lean (while standing or seated) with their head into the fume hood/bio safety cabinet to get a top down view of the biopsies and excisions (performing inking, describing markers, and slicing samples). This posture poses a significant risk of injury on the back and neck thus the need for a workstation adjustment. The risk of inhalation is low, but present, so that is a minor concern as well. Initial Thoughts: 1. Rotate the sample to face the histologist, like reading a book. 2. Affix the sample to a cutting board (or the surface currently in use) by means of a reverse action tweezers mounted to the board. 3. At this angle the histotech can see the sample with minimal neck bending (while keeping the head out of the hood) as well as reduce the need to hold the sample while performing inking, etc. Questions: 1. Has anyone tried this? 2. Does anyone else have a solution that would work in this situation? 3. What is your opinion on this solution? Any ideas or comments are greatly welcome. Thank you! Ian Ian Bertmaring, MS, AEP - Ergonomist Department of Environment, Health & Safety 1120 Estes Drive Extension, CB #1650 Chapel Hill, NC 27599 - 1650 Phone: (919) 843 - 4642; Fax: (919) 962 - 0227 ergonomics@ehs.unc.edu http://www.ehs.unc.edu/workplace_safety/ergonomics/ From PMonfils <@t> Lifespan.org Mon Dec 11 16:07:08 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Mon Dec 11 16:07:20 2006 Subject: [Histonet] Grossing postures and proceedures - Ergonomics In-Reply-To: <79ACF88C02E12F419FE8ECA36001D8FAA3315F@auxsexc1.aux-services.unc.edu> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273BCF@LSRIEXCH1.lsmaster.lifespan.org> Possibly an angled mirror over the specimen? Bottom edge of the mirror in contact with the benchtop, behind the specimen; mirror leaning toward the tech at a suitable angle? This would give the tech a perpendicular view of the specimen with a normal angle of view for the tech. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bertmaring,Ian (Environment Health & Safety) > Sent: Monday, December 11, 2006 1:46 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Grossing postures and proceedures - Ergonomics > > I have a situation that I am looking for a solution for and I figured > some one in this community could help me. > > The Problem: > When techs at UNC perform grossing, they often lean (while standing or > seated) with their head into the fume hood/bio safety cabinet to get a > top down view of the biopsies and excisions (performing inking, > describing markers, and slicing samples). This posture poses a > significant risk of injury on the back and neck thus the need for a > workstation adjustment. The risk of inhalation is low, but present, so > that is a minor concern as well. > > Initial Thoughts: > 1. Rotate the sample to face the histologist, like reading a book. > 2. Affix the sample to a cutting board (or the surface currently in > use) by means of a reverse action tweezers mounted to the board. > 3. At this angle the histotech can see the sample with minimal neck > bending (while keeping the head out of the hood) as well as reduce the > need to hold the sample while performing inking, etc. > > Questions: > 1. Has anyone tried this? > 2. Does anyone else have a solution that would work in this situation? > 3. What is your opinion on this solution? > > Any ideas or comments are greatly welcome. > > Thank you! > > Ian > > > Ian Bertmaring, MS, AEP - Ergonomist > Department of Environment, Health & Safety > 1120 Estes Drive Extension, CB #1650 > Chapel Hill, NC 27599 - 1650 > > Phone: (919) 843 - 4642; Fax: (919) 962 - 0227 > > ergonomics@ehs.unc.edu > > http://www.ehs.unc.edu/workplace_safety/ergonomics/ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From NMargaryan <@t> childrensmemorial.org Mon Dec 11 16:42:36 2006 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Mon Dec 11 16:43:24 2006 Subject: [Histonet] RE: Polymer Feedback? Message-ID: <63B8B599DE283148B92E83C78B32C15D03C04FF5@cmhexbe2.childrensmemorial.org> Hello, I never used polymer. My questions are: 1. Is it has to melt as paraffin, if so what degree? 2. What is the depolymerization protocol? 3. Is it requires Antigen retrieval step? 4. Why it's not requires Avidin/Biotin step? Thank you, Naira Naira V. Margaryan, D.V.M., Ph.D. Research Associate III Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3394 Tel: 773-755-6570/ext-8 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Friday, December 08, 2006 10:29 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 37, Issue 9 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Automated stainer (Johnson, Teri) (Jerry Helisek) 2. RE: Polymer Feedback? (Dawson, Glen) 3. Re: paraffin problem (rsrichmond@aol.com) 4. RE: Polymer Feedback? (Bonnie Whitaker) 5. RE: Polymer Feedback? (Horn, Hazel V) 6. looking for someone to do a Brucella suis IHC on Caribou (HCS) 7. looking for a slide carrier for Shandon X-Y stainer (HCS) 8. RE: Polymer Feedback? (patsy ruegg) 9. RE: Re: paraffin problem (patsy ruegg) 10. RE: Polymer Feedback? (patsy ruegg) 11. Shur/mark slides (Kimberly Johnson) 12. GFP and B-Gal positive control slides (Rebecca Nishi) 13. RE: GFP and B-Gal positive control slides (Koelling, Ray) 14. Fading (histotech@charter.net) 15. RE: neurospheres (Tarango, Mark) 16. ICAM-1 Antibody (Wulan Anggrahini) 17. cryostat whisperer???... (i need parts for a Leica, STAT) (Debbie Keith) 18. RE: Fading (Kemlo Rogerson) 19. Re: Fading (Rene J Buesa) 20. Microscope slide trays -- not folders (Victoria Baker) 21. RE: Microscope slide trays -- not folders (Horn, Hazel V) 22. cryostat whisperer???... (i need parts for a Leica, STAT) (Stephen Peters M.D.) 23. RE: Shur/mark slides (Kellar, Eric C) 24. employment (Bonnie Whitaker) 25. CD 79 antibody (Colin Nixon) 26. Re: CD 79 antibody (Rene J Buesa) 27. digital image analysis systems... (Breckenridge, Richard A) ---------------------------------------------------------------------- Message: 1 Date: Thu, 7 Dec 2006 13:22:16 -0500 From: "Jerry Helisek" Subject: [Histonet] Automated stainer (Johnson, Teri) To: Message-ID: <3855F92002259948A66A8CA2D16E3A4F017BB3@server.ralambusa.com> Content-Type: text/plain; charset="us-ascii" Teri, Please see: http://www.ralamb.net/product_info.php?products_id=595&osCsid=52e93ef809 10bcc854ff5818dc168ef6 I think this may be what you are looking for in a small stainer (RA Lamb's StainMate) Jerry Helisek 5409 Lumley Road, Unit #102 Durham, North Carolina 27703 Phone: 919-957-1964 Fax: 919-957-1972 Cell: 919-264-7964 jerry@ralambusa.com www.ralamb.com The information contained in this e-mail message may be privileged, confidential and protected from disclosure. If you are not the intended recipient, any dissemination, distribution or copying is strictly prohibited. If you think that you have received this e-mail message in error please e-mail the sender and delete the message. Thank you. ------------------------------ Message: 2 Date: Thu, 7 Dec 2006 12:24:51 -0600 From: "Dawson, Glen" Subject: RE: [Histonet] Polymer Feedback? To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Doug, Polymers Rock!!! No avidin/biotin blocking. Better sensitivity so you save some of their extra cost by taking antibody titers out farther. Clean. Fewer steps. So on and so on. Only bad thing is the cost but nothing is perfect. Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Doug Geddes Sent: Thursday, December 07, 2006 11:48 AM To: Histonet@lists.utsouthwestern.edu; Gayle Callis Subject: Re: [Histonet] Polymer Feedback? Sorry, polymers for IHC staining. Doug >>> Gayle Callis 2006-12-07 12:36 PM >>> Doug, Polymers for what purpose, embedding tissues? At 09:22 AM 12/7/2006, you wrote: >Dear Histonet, > >Looking for feedback and experiences with different ploymers >(preferably universal) in a clinical setting. > >Doug Geddes BSc, MLT >Dept of Pathology >LHSC London ON >Canada Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 ----------------------------------------- This information is directed in confidence solely to the person named above and may contain confidential and/or privileged material. This information may not otherwise be distributed, copied or disclosed. If you have received this e-mail in error, please notify the sender immediately via a return e-mail and destroy original message. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Thu, 07 Dec 2006 13:36:53 -0500 From: rsrichmond@aol.com Subject: [Histonet] Re: paraffin problem To: histonet@lists.utsouthwestern.edu Message-ID: <8C8E845CD1DB9FA-250-41C1@MBLK-M23.sysops.aol.com> Content-Type: text/plain; charset="us-ascii" Several people comment on problems with present day embedding waxes ("paraffin"). Geezer time. In the more than forty years I've been a pathologist, one of the biggest improvements in technology has been the replacement of simple paraffin waxes with the present proprietary mixtures. In the 1960's, many laboratories simply bought Gulfwax - the wax used by home canners to seal canning jars. For quite a few years now complex mixtures of who knows what have replaced simple paraffin. These mixtures are all proprietary and trade-secret, and as far as I know this whole change has been largely undocumented in the literature. But the improvement in sections that has resulted has been revolutionary. In the 1960's many laboratories were simply unable to cut an interpretable section of a lymph node. Frank Foote, then the chief of surgical pathology at Memorial/Sloan Kettering in New York city, often observed that most of a surgical pathology consultant's expertise was in interpreting nearly unreadable slides. Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. ------------------------------ Message: 4 Date: Thu, 7 Dec 2006 13:06:39 -0600 From: "Bonnie Whitaker" Subject: RE: [Histonet] Polymer Feedback? To: "'Dawson, Glen'" , Message-ID: <000a01c71a32$d30055d0$3601a8c0@brownpathology.net> Content-Type: text/plain; charset="us-ascii" Like a boss used to tell me: You can have better quality, you can have a faster turnaround time or you can produce a product that is cheaper. Pick 2. You can never have all three! Polymers are faster and better (my favorite pick for the 2) Bonnie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Thursday, December 07, 2006 12:25 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Polymer Feedback? Doug, Polymers Rock!!! No avidin/biotin blocking. Better sensitivity so you save some of their extra cost by taking antibody titers out farther. Clean. Fewer steps. So on and so on. Only bad thing is the cost but nothing is perfect. Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Doug Geddes Sent: Thursday, December 07, 2006 11:48 AM To: Histonet@lists.utsouthwestern.edu; Gayle Callis Subject: Re: [Histonet] Polymer Feedback? Sorry, polymers for IHC staining. Doug >>> Gayle Callis 2006-12-07 12:36 PM >>> Doug, Polymers for what purpose, embedding tissues? At 09:22 AM 12/7/2006, you wrote: >Dear Histonet, > >Looking for feedback and experiences with different ploymers >(preferably universal) in a clinical setting. > >Doug Geddes BSc, MLT >Dept of Pathology >LHSC London ON >Canada Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 ----------------------------------------- This information is directed in confidence solely to the person named above and may contain confidential and/or privileged material. This information may not otherwise be distributed, copied or disclosed. If you have received this e-mail in error, please notify the sender immediately via a return e-mail and destroy original message. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 5 Date: Thu, 7 Dec 2006 13:45:07 -0600 From: "Horn, Hazel V" Subject: RE: [Histonet] Polymer Feedback? To: "Doug Geddes" , Histonet@lists.utsouthwestern.edu Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6EFF@EMAIL.archildrens.org> Content-Type: text/plain; charset=us-ascii I'm interested in polymers as well. This is new for me. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Doug Geddes Sent: Thursday, December 07, 2006 10:23 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Polymer Feedback? Dear Histonet, Looking for feedback and experiences with different ploymers (preferably universal) in a clinical setting. Doug Geddes BSc, MLT Dept of Pathology LHSC London ON Canada ----------------------------------------- This information is directed in confidence solely to the person named above and may contain confidential and/or privileged material. This information may not otherwise be distributed, copied or disclosed. If you have received this e-mail in error, please notify the sender immediately via a return e-mail and destroy original message. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== ------------------------------ Message: 6 Date: Thu, 7 Dec 2006 12:27:13 -0800 From: "HCS" Subject: [Histonet] looking for someone to do a Brucella suis IHC on Caribou To: "Histonet" Message-ID: <000601c71a3e$16ca4e00$6701a8c0@hp> Content-Type: text/plain; charset="iso-8859-1" Hi, I would like to find someone who does IHC that could do a Brucella suis stain on a Caribou. Please email me if you do this stain or know of a contact for me. Thanks LeRoy Brown HT(ASCP) HTL HCS, Inc. 360-966-7300 ------------------------------ Message: 7 Date: Thu, 7 Dec 2006 12:29:14 -0800 From: "HCS" Subject: [Histonet] looking for a slide carrier for Shandon X-Y stainer To: "Histonet" Message-ID: <000601c71a3e$5cf1e640$6701a8c0@hp> Content-Type: text/plain; charset="iso-8859-1" Does anyone have an extra slide carrier that fits the Shandon x-y stainer? thanks LeRoy Brown Ht(ASCP) Htl HCS, Inc. 360-966-7300 ------------------------------ Message: 8 Date: Thu, 7 Dec 2006 13:54:10 -0700 From: "patsy ruegg" Subject: RE: [Histonet] Polymer Feedback? To: "'Bonnie Whitaker'" , "'Dawson, Glen'" , Message-ID: <200612072054.kB7KsBen082197@pro12.abac.com> Content-Type: text/plain; charset="us-ascii" Bonnie, You are generous, my boss always said "pick one" out of the three you mentioned. In the case of labeled polymer I think you are right, they are faster and I think better, at least I do not have to worry about endogenous biotin issues. They an't cheap though. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Bonnie Whitaker Sent: Thursday, December 07, 2006 12:07 PM To: 'Dawson, Glen'; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Polymer Feedback? Like a boss used to tell me: You can have better quality, you can have a faster turnaround time or you can produce a product that is cheaper. Pick 2. You can never have all three! Polymers are faster and better (my favorite pick for the 2) Bonnie -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Thursday, December 07, 2006 12:25 PM To: Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Polymer Feedback? Doug, Polymers Rock!!! No avidin/biotin blocking. Better sensitivity so you save some of their extra cost by taking antibody titers out farther. Clean. Fewer steps. So on and so on. Only bad thing is the cost but nothing is perfect. Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Doug Geddes Sent: Thursday, December 07, 2006 11:48 AM To: Histonet@lists.utsouthwestern.edu; Gayle Callis Subject: Re: [Histonet] Polymer Feedback? Sorry, polymers for IHC staining. Doug >>> Gayle Callis 2006-12-07 12:36 PM >>> Doug, Polymers for what purpose, embedding tissues? At 09:22 AM 12/7/2006, you wrote: >Dear Histonet, > >Looking for feedback and experiences with different ploymers >(preferably universal) in a clinical setting. > >Doug Geddes BSc, MLT >Dept of Pathology >LHSC London ON >Canada Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 ----------------------------------------- This information is directed in confidence solely to the person named above and may contain confidential and/or privileged material. This information may not otherwise be distributed, copied or disclosed. If you have received this e-mail in error, please notify the sender immediately via a return e-mail and destroy original message. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Thu, 7 Dec 2006 13:57:26 -0700 From: "patsy ruegg" Subject: RE: [Histonet] Re: paraffin problem To: , Message-ID: <200612072057.kB7KvRp5083970@pro12.abac.com> Content-Type: text/plain; charset="us-ascii" I agree Samurai, the better paraffin and disposable knives have changed paraffin sectioning forever for better. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of rsrichmond@aol.com Sent: Thursday, December 07, 2006 11:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: paraffin problem Several people comment on problems with present day embedding waxes ("paraffin"). Geezer time. In the more than forty years I've been a pathologist, one of the biggest improvements in technology has been the replacement of simple paraffin waxes with the present proprietary mixtures. In the 1960's, many laboratories simply bought Gulfwax - the wax used by home canners to seal canning jars. For quite a few years now complex mixtures of who knows what have replaced simple paraffin. These mixtures are all proprietary and trade-secret, and as far as I know this whole change has been largely undocumented in the literature. But the improvement in sections that has resulted has been revolutionary. In the 1960's many laboratories were simply unable to cut an interpretable section of a lymph node. Frank Foote, then the chief of surgical pathology at Memorial/Sloan Kettering in New York city, often observed that most of a surgical pathology consultant's expertise was in interpreting nearly unreadable slides. Bob Richmond Samurai Pathologist Knoxville TN ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Thu, 7 Dec 2006 13:45:08 -0700 From: "patsy ruegg" Subject: RE: [Histonet] Polymer Feedback? To: "'Doug Geddes'" , Message-ID: <200612072045.kB7Kj8H3077358@pro12.abac.com> Content-Type: text/plain; charset="us-ascii" Do you mean labeled polymers for ihc? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Doug Geddes Sent: Thursday, December 07, 2006 9:23 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Polymer Feedback? Dear Histonet, Looking for feedback and experiences with different ploymers (preferably universal) in a clinical setting. Doug Geddes BSc, MLT Dept of Pathology LHSC London ON Canada ----------------------------------------- This information is directed in confidence solely to the person named above and may contain confidential and/or privileged material. This information may not otherwise be distributed, copied or disclosed. If you have received this e-mail in error, please notify the sender immediately via a return e-mail and destroy original message. Thank you for your cooperation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 11 Date: Thu, 7 Dec 2006 13:09:01 -0800 From: "Kimberly Johnson" Subject: [Histonet] Shur/mark slides To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hello! I finally got myself an automated slide labeler! YAY! But I need some help on what slides to use. I have the Shur/mark labeler from TBS, and got some slides, but I realized I needed to get some positively charged slides. I know a lot of you use this machine and was wondering if you all could guide me towards a good slide that is charged that works with the labeler. Thank you so much for your time, your advise is always great! Kim Kimberly Johnson Research Associate II CovX Ph. (858)964-2050 Fax (858)964-2090 The information contained in this e-mail message may be privileged and confidential information and is intended only for the use of the individual and/or entity identified in the alias address of this message. If the reader of this message is not the intended recipient, or an employee or agent responsible to deliver it to the intended recipient, you are hereby requested not to distribute or copy this communication. If you have received this communication in error, please notify us immediately by telephone or return e-mail and delete the original message from your system. ------------------------------ Message: 12 Date: Thu, 7 Dec 2006 13:34:17 -0800 From: Rebecca Nishi Subject: [Histonet] GFP and B-Gal positive control slides To: histonet@lists.utsouthwestern.edu Message-ID: <4B87DFCC-BE93-449B-85A0-CCF5C15AA67C@uci.edu> Content-Type: text/plain; charset=US-ASCII; delsp=yes; format=flowed Does anyone know where I can buy, or can suggest an easy way to make positive control slides for GFP and B-Gal. We have use AAV contstructs with either GFP or LAC-Z and injected into mice. We have sectioned the tissue and would like to look for positive GFP or B-Gal. It would be nice to have a positive control slide. We plan on visualizing both with and without antibody amplification. I would appreciate any suggestions. Thank You. Rebecca ------------------------------------ Rebecca Nishi UC Irvine Christopher Reeve Foundation SCI Core/Anderson Lab 1226 GNRF Irvine, CA 92697-4540 rnishi@uci.edu Phone/Fax: 949-824-9728 ------------------------------ Message: 13 Date: Thu, 7 Dec 2006 14:05:03 -0800 From: "Koelling, Ray" Subject: RE: [Histonet] GFP and B-Gal positive control slides To: Rebecca Nishi , histonet@lists.utsouthwestern.edu Message-ID: <16834C6DFFA6004C88DE4507FB8AE544059839E9@wa-mb4-sea.amgen.com> Content-Type: text/plain Rebecca, For GFP, that can be a can of worms. For B-Gal this is what we do. From mouse vendor, get a Tie-2/lacZ transgenic mouse strain. Are readily available commercially. Tie-2 is expressed in endothelium and the lacZ reporter gene is co-expressed there. Take any tissue (kid/liver/whatever). B-Gal antibodies show beautifully stained endothelium. Ray Raymond Koelling Research Scientist Amgen Seattle -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rebecca Nishi Sent: Thursday, December 07, 2006 1:34 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GFP and B-Gal positive control slides Does anyone know where I can buy, or can suggest an easy way to make positive control slides for GFP and B-Gal. We have use AAV contstructs with either GFP or LAC-Z and injected into mice. We have sectioned the tissue and would like to look for positive GFP or B-Gal. It would be nice to have a positive control slide. We plan on visualizing both with and without antibody amplification. I would appreciate any suggestions. Thank You. Rebecca ------------------------------------ Rebecca Nishi UC Irvine Christopher Reeve Foundation SCI Core/Anderson Lab 1226 GNRF Irvine, CA 92697-4540 rnishi@uci.edu Phone/Fax: 949-824-9728 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Thu, 7 Dec 2006 15:59:38 -0800 From: Subject: [Histonet] Fading To: histonet Message-ID: <1064608487.1165535978746.JavaMail.root@fepweb05> Content-Type: text/plain; charset=utf-8 Hello, For the past few weeks, we've been having problems with our slides fading approximately one week after they are stained. Any ideas on what's causing this and how to correct the situation? Thank you, DDietz Morristown-Hamblen Lab ------------------------------ Message: 15 Date: Thu, 7 Dec 2006 16:38:40 -0800 From: "Tarango, Mark" Subject: RE: [Histonet] neurospheres To: "Atoska Gentry" , "Histonet" Message-ID: <5AEC610C1CE02945BD63A395BA763EDEE009BE@NVCIEXCH02.NVCI.org> Content-Type: text/plain; charset=us-ascii When I take the chamber/divider thing off the slide, I make sure to remove the media first (and very carefully so the cells don't come off the slide). Then I make sure to let it air dry for plenty of time before I do anything to it. If she is trying to use chamber slides on a cell culture, I hope they are adherent cells. If they are the kind of cells that grow in suspension (like hematopoietic cells), then you won't many cells adhere. I don't know if neurospheres will adhere... Mark Adam Tarango HT(ASCP) Histology/Immunohistochemistry Supervisor Nevada Cancer Institute One Breakthrough Way Las Vegas, NV 89135 mtarango@nvcancer.org Direct Line (702) 822-5112 Fax (702) 939-7663 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Atoska Gentry Sent: Thursday, December 07, 2006 8:19 AM To: Histonet Subject: [Histonet] neurospheres Hello, does anyone have a protocol for fixation & staining neurospheres from tissue culture either on chamber slides or cryosections? If so will you be so kind as to share it with me ASAP? One of our researchers here has attempted using the chamber slides but, she said the cells all washed off. Technique / protocol suggestions will be much appreciated. Thanks, Atoska -- Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet "EMF " made the following annotations. ------------------------------------------------------------------------------ CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this e-mail message or its attachments. If you believe you have received this e-mail message in error, please contact the sender by reply e-mail and destroy all copies of the original message ============================================================================== ------------------------------ Message: 16 Date: Fri, 8 Dec 2006 10:45:21 +0900 From: "Wulan Anggrahini" Subject: [Histonet] ICAM-1 Antibody To: Message-ID: <001a01c71a6a$85cc4200$0bc8a8c0@your56ab121f6e> Content-Type: text/plain; charset="iso-8859-1" Hi.. I need information about good purified rat anti mouse ICAM-1 monoclonal antibody. I want to buy from Chemicon but there are apparently two types of clones from Chemicon for rat antimouse ICAM-1. CLone KAT-1 and Clone LTF653. Does anyone has any experience on using this antibody ? I need to stain mouse vascular tissue on frozen section with 4% Paraformaldehyde fixation. Thanks a lot for the help. Regards, Wulan Anggrahini Kobe University ------------------------------ Message: 17 Date: Thu, 07 Dec 2006 20:11:44 -0700 From: Debbie Keith Subject: [Histonet] cryostat whisperer???... (i need parts for a Leica, STAT) To: histonet@lists.utsouthwestern.edu Message-ID: <5.2.0.9.0.20061207195737.00ba7908@pop.central.cox.net> Content-Type: text/plain; charset=us-ascii; format=flowed; x-avg-checked=avg-ok-3C9AC07 i'd like to thank everyone for their input about my recent cryostat issues... the biomedical repair-folk came out on wednesday and diagnosed the issue. it seems the screw/lever on the chuck assembly (that tightens the chuck) has been over-tightened to the point of stripping the threads and rendering it chronically "un-tight". the harder it's tightened... the harder it has to be tightened. one of the repair-folk actually had what MUST have been a "Viola Moment". he tightened the handle and it sectioned like a spanky-new machine would. it was beautiful. after he left, i cut the next block... and had to adjust the chuck. keep in mind that this repair guy is a STRAPPING person that could EASILY haul large farm equipment with the best of them.... and i have the upper body strength of the average 3 year-old. after much crying, causing ice crystals to form and a hush to fall... i called the repair guy. he told me it was going to take 2-4 weeks for the parts to come from Leica. is there anyone out there that KNOWS where to get the parts quicker? i would like to note... the repair guy is pretty good. he doesn't make much conversation... but he "listens" to the machine. he said, without even looking at the machine, "i listen for the sound the machine makes when it's cutting 'right'." later, when it WAS cutting right... he said "do you hear that?? huh? do you hear THAT???". it DID make a very distinct sound. next time you make a beautiful section... listen for the "sssschhhuctt" sound. THEN, think of me crying into my cm1510. (seriously, any ideas about parts?) debbie -- No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.1.409 / Virus Database: 268.15.15/579 - Release Date: 12/7/2006 ------------------------------ Message: 18 Date: Fri, 8 Dec 2006 08:22:36 -0000 From: "Kemlo Rogerson" Subject: RE: [Histonet] Fading To: , "histonet" Message-ID: <86ADE4EB583CE64799A9924684A0FBBF01246F79@wahtntex2.waht.swest.nhs.uk> Content-Type: text/plain; charset="us-ascii" Would be useful to know which stain is fading. I assume H&E and in that case which element, the 'H' or the 'E'? Causes are different. Kemlo Rogerson Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo.rogerson@nhs.net Once you are in the orbit of your destiny, weightlessness is the only result. --Baba Amte This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation ------------------------------ Message: 19 Date: Fri, 8 Dec 2006 05:29:50 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] Fading To: histotech@charter.net, histonet Message-ID: <747142.68162.qm@web61217.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Even when theoretically after dehydration is completed all the water should have been removed from the section, IF the tap water used after the hamoxylin is "reach" in chlorine, those ions will react with the dyes and will make them to fave even after the sections have been coverslipped. Check for chlorine in your tap water supply. Ren? J. histotech@charter.net wrote: Hello, For the past few weeks, we've been having problems with our slides fading approximately one week after they are stained. Any ideas on what's causing this and how to correct the situation? Thank you, DDietz Morristown-Hamblen Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Everyone is raving about the all-new Yahoo! Mail beta. ------------------------------ Message: 20 Date: Fri, 8 Dec 2006 09:29:10 -0500 From: "Victoria Baker" Subject: [Histonet] Microscope slide trays -- not folders To: "Histo Net list server" Message-ID: <4f016b690612080629h1f035e45od6501842298f395@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Happy Friday Everyone! Thanks to everyone who responded to my e-mail about American Histo Labs, it was a big help. Now, I'm searching for slide trays that are about 14"long and 11-1/2" wide. Some are wood others are an acrylic I think. They have 4 rows and hold a lot of slides. I've been trying to find them through my usual sources without success. Any information is welcomed. Have a nice weekend. Vikki ------------------------------ Message: 21 Date: Fri, 8 Dec 2006 09:07:53 -0600 From: "Horn, Hazel V" Subject: RE: [Histonet] Microscope slide trays -- not folders To: "Victoria Baker" , "Histo Net list server" Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6F01@EMAIL.archildrens.org> Content-Type: text/plain; charset=us-ascii Try www.brainlab.com They have them. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Victoria Baker Sent: Friday, December 08, 2006 8:29 AM To: Histo Net list server Subject: [Histonet] Microscope slide trays -- not folders Happy Friday Everyone! Thanks to everyone who responded to my e-mail about American Histo Labs, it was a big help. Now, I'm searching for slide trays that are about 14"long and 11-1/2" wide. Some are wood others are an acrylic I think. They have 4 rows and hold a lot of slides. I've been trying to find them through my usual sources without success. Any information is welcomed. Have a nice weekend. Vikki _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== ------------------------------ Message: 22 Date: Fri, 8 Dec 2006 07:18:58 -0800 (PST) From: "Stephen Peters M.D." Subject: [Histonet] cryostat whisperer???... (i need parts for a Leica, STAT) To: Histonet@lists.utsouthwestern.edu Message-ID: <466458.27471.qm@web30404.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Dear Debbie, Try my friends at Belair Instrument Company. 973 912 8900. They sell and service these and I expect they would have something in stock to help you. Stephen Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com ------------------------------ Message: 23 Date: Fri, 8 Dec 2006 10:35:52 -0500 From: "Kellar, Eric C" Subject: RE: [Histonet] Shur/mark slides To: histonet@lists.utsouthwestern.edu Message-ID: <6843061CE6B98E4B96590D4F299618F801583BF0@qdcws0117.us.qdx.com> Content-Type: text/plain; charset=iso-8859-1 Kim, I have two Shur/mark slides etchers that run all day long. Most Colorfrost Plus slides in darker colors - blue/green/tan available from Cardinal, Thermo Fisher, Lamb all work well. I have also tried Shur/mark brand Colorfrost Plus slides, they come with a color painted background on the opposite side of the slide to enhance visibility. They are very nice, but are extremely overpriced. It's the diamond stylus that needs to be replaced often with any frosted slide you choose. They're expensive too and come in a five pack, so stock up. Eric C. Kellar Quest Diagnostics, Inc Histology Laboratory Supervisor Miami -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kimberly Johnson Sent: Thursday, December 07, 2006 4:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Shur/mark slides Hello! I finally got myself an automated slide labeler! YAY! But I need some help on what slides to use. I have the Shur/mark labeler from TBS, and got some slides, but I realized I needed to get some positively charged slides. I know a lot of you use this machine and was wondering if you all could guide me towards a good slide that is charged that works with the labeler. Thank you so much for your time, your advise is always great! Kim Kimberly Johnson Research Associate II CovX Ph. (858)964-2050 Fax (858)964-2090 The information contained in this e-mail message may be privileged and confidential information and is intended only for the use of the individual and/or entity identified in the alias address of this message. If the reader of this message is not the intended recipient, or an employee or agent responsible to deliver it to the intended recipient, you are hereby requested not to distribute or copy this communication. If you have received this communication in error, please notify us immediately by telephone or return e-mail and delete the original message from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ============================================================================== The contents of this message, together with any attachments, are intended only for the use of the person(s) to which they are addressed and may contain confidential and/or privileged information. Further, any medical information herein is confidential and protected by law. It is unlawful for unauthorized persons to use, review, copy, disclose, or disseminate confidential medical information. If you are not the intended recipient, immediately advise the sender and delete this message and any attachments. Any distribution, or copying of this message, or any attachment, is prohibited. ============================================================================== ------------------------------ Message: 24 Date: Fri, 8 Dec 2006 09:54:09 -0600 From: "Bonnie Whitaker" Subject: [Histonet] employment To: Message-ID: <002d01c71ae1$18f9b410$3601a8c0@brownpathology.net> Content-Type: text/plain; charset="us-ascii" Hi All, A histotech here in the Houston area asked me to place a post on her behalf. She is looking for employment, temporary or permanent. The Houston area would be great, but she will consider other areas. She does not want to work the night shift, however. If you have a position available, please email me off-line and I will forward the messages. Thanks, Bonnie Whitaker Lab Manager Brown & Associates Medical Laboratories 8076 El Rio Houston, Texas 77054 713-741-6677 ------------------------------ Message: 25 Date: Fri, 8 Dec 2006 16:01:50 -0000 From: "Colin Nixon" Subject: [Histonet] CD 79 antibody To: Message-ID: <002601c71ae2$2bd18170$d7e9d182@vet.gla.ac.uk> Content-Type: text/plain; charset="iso-8859-1" Does anyone know of a suitable CD79/B cell marker that works with Immunohistochemistry on formalin fixed paraffin embedded mice tissue? Colin ------------------------------ Message: 26 Date: Fri, 8 Dec 2006 08:08:25 -0800 (PST) From: Rene J Buesa Subject: Re: [Histonet] CD 79 antibody To: Colin Nixon , Histonet@lists.utsouthwestern.edu Message-ID: <412456.26375.qm@web61221.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I used CD79a from Novocastra (1:25 dil pH6 HIER), tonsil control. Ren? J. Colin Nixon wrote: Does anyone know of a suitable CD79/B cell marker that works with Immunohistochemistry on formalin fixed paraffin embedded mice tissue? Colin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Check out the all-new Yahoo! Mail beta - Fire up a more powerful email and get things done faster. ------------------------------ Message: 27 Date: Fri, 8 Dec 2006 10:11:41 -0600 From: "Breckenridge, Richard A" Subject: [Histonet] digital image analysis systems... To: Message-ID: Content-Type: text/plain; charset="us-ascii" Hi all, Just looking for feedback on image analysis systems that are out there. I plan on demo-ing some and any info is welcome. Thanks! Rick Richard A. Breckenridge, HT(ASCP) Technical Director/ Chief Histology Technician University of Texas- Houston Medical School Histology/ IHC Laboratory Office 713-500-6792 IHC Lab 713-500-5096 Histology Lab 713-500-5363 FAX 713-500-0733 Email - Richard.Breckenridge@uth.tmc.edu ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 37, Issue 9 *************************************** From stecker <@t> salk.edu Mon Dec 11 17:22:23 2006 From: stecker <@t> salk.edu (Kim Stecker) Date: Mon Dec 11 17:27:43 2006 Subject: [Histonet] used equipment Message-ID: Dear Roxanne, How much are you asking for the microtomes and embedding machines?? Kim Kim Stecker The Salk Instutue of Biological Sciences 10010 N. Torrey Pines Rd. La Jolla, CA 92037 Phone: 858-453-4100 X1010 Fax: 858-597-0824 email: stecker@salk.edu From stecker <@t> salk.edu Mon Dec 11 17:31:04 2006 From: stecker <@t> salk.edu (Kim Stecker) Date: Mon Dec 11 17:36:15 2006 Subject: [Histonet] ALK-1 Controls Message-ID: <4D168535-5A2A-4BE6-A18F-83EF43F5C8B3@salk.edu> Someone asked about ALK-1 controls about two weeks ago and I have some available for $300.00 per block. Kim Stecker The Salk Instutue of Biological Sciences 10010 N. Torrey Pines Rd. La Jolla, CA 92037 Phone: 858-453-4100 X1010 Fax: 858-597-0824 email: stecker@salk.edu From annabelmazza <@t> yahoo.com Mon Dec 11 21:18:17 2006 From: annabelmazza <@t> yahoo.com (Annabel L. Mazza) Date: Mon Dec 11 21:18:25 2006 Subject: [Histonet] Regarding HT exam & GrandFathering provision Message-ID: <41149.89538.qm@web35701.mail.mud.yahoo.com> Hi : Does anyone know if the High School diploma/9 years full time histology experience route that is needed to take the HT exam. or will prospective candidates be "grandfathered in?" Thanks in advance for the information. A. Mazza __________________________________________________ Correo Yahoo! Espacio para todos tus mensajes, antivirus y antispam ?gratis! Reg?strate ya - http://correo.espanol.yahoo.com/ From Asf2k3 <@t> aol.com Mon Dec 11 22:42:08 2006 From: Asf2k3 <@t> aol.com (Asf2k3@aol.com) Date: Mon Dec 11 22:42:23 2006 Subject: [Histonet] Re: Histonet Digest, Vol 37, Issue 13 Message-ID: Hi, Does any one know any good histology websites where they show cartoon person cutting,give you hints and advice on the microtome, embedding? A.S From Asf2k3 <@t> aol.com Mon Dec 11 22:45:18 2006 From: Asf2k3 <@t> aol.com (Asf2k3@aol.com) Date: Mon Dec 11 22:45:32 2006 Subject: [Histonet] Cutting and embedding Message-ID: Does anyone know any good histology websites where they show like a cartoon person cutting on a microtome and embedding, and give advice and hints on such topics. A.S From GAshton <@t> PICR.man.ac.uk Tue Dec 12 02:50:40 2006 From: GAshton <@t> PICR.man.ac.uk (Garry Ashton) Date: Tue Dec 12 02:50:56 2006 Subject: [Histonet] cryostat Message-ID: Dear all, We have an old cryostat that is surplus to requirements (Richert Jung Cryocut E) It has not been plugged in for a couple of years, but when I tried it yesterday it seemed to get down to temperature. However I can't guarantee it works OK. There are also the transport costs to consider. However if anybody in the UK would like it, then let me know. Many thanks Garry Garry Ashton PICR Manchester UK -------------------------------------------------------- This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the University of Manchester. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From campisi <@t> ipmc.cnrs.fr Tue Dec 12 03:43:54 2006 From: campisi <@t> ipmc.cnrs.fr (campisi@ipmc.cnrs.fr) Date: Tue Dec 12 03:40:12 2006 Subject: [Histonet] Unsubscribed Message-ID: <3165.193.51.225.52.1165916634.squirrel@mail.ipmc.cnrs.fr> From ree3 <@t> leicester.ac.uk Tue Dec 12 04:12:30 2006 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Tue Dec 12 04:12:44 2006 Subject: [Histonet] Cutting and embedding In-Reply-To: Message-ID: My uncanny resemblance to "Popeye" has been remarked upon a number of times, perhaps you would like to come and film me at work?. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Asf2k3@aol.com Sent: 12 December 2006 04:45 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cutting and embedding Does anyone know any good histology websites where they show like a cartoon person cutting on a microtome and embedding, and give advice and hints on such topics. A.S _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Tue Dec 12 09:09:52 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Dec 12 09:10:09 2006 Subject: [Histonet] floaters In-Reply-To: References: <6.0.0.22.1.20061211094306.01d50970@gemini.msu.montana.edu> Message-ID: <6.0.0.22.1.20061212080725.01b1bc70@gemini.msu.montana.edu> Well, I had my long hair fall into a waterbath many years ago, also into paraffin when embedding. I suggest shampooing with Head and Shoulders to prevent dandruff!! Gayle Callis At 09:15 PM 12/11/2006, you wrote: >Gail > >You left out dandruff, Im sure that is a prime source of non nucleated >squames on slides. > >David M. Peck HT,HTL(ASCP) From amylee779 <@t> yahoo.com Tue Dec 12 09:12:21 2006 From: amylee779 <@t> yahoo.com (Amy Lee) Date: Tue Dec 12 09:12:30 2006 Subject: [Histonet] Please,how to explain this IHC data? Message-ID: <20061212151221.30737.qmail@web38012.mail.mud.yahoo.com> Hello, I have a IHC problem that keeps bothering me. I am asking the help from the expert here. Tissue: human glioblastoma grow in mouse brain, frozen or FFPE. Antibody is human antibody. This antibody gave pretty stain in frozen tissue in glioblastoma area while the mouse brain area is negative. I was very happy to see that. But later when I did human IgG control it gave the same staining pattern as my primary antibody. However, when I did same thing on FFPE tissue, they are all negative for my antibody or hIgG. I am having trouble to explain this. Could anybody help me explain this? If the staining I got from my primary antibody is all background, why only human glio has the staining? Why do frozen and FFPE tissue have such different results? I appreciate any inputs on this! --------------------------------- Need a quick answer? Get one in minutes from people who know. Ask your question on Yahoo! Answers. From slappycraw <@t> yahoo.com Tue Dec 12 09:22:15 2006 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Tue Dec 12 09:22:25 2006 Subject: [Histonet] Looking for Chris Van der Loos Message-ID: <20061212152215.90870.qmail@web53602.mail.yahoo.com> Hi: I tried to order a book ( Immunoenzyme Multiple Staining Methods) by Chris Van Der Loos but my order came back saying out of print and unavailable. Anyone out there know of where we could score a copy of this book? Thanks --------------------------------- Check out the all-new Yahoo! Mail beta - Fire up a more powerful email and get things done faster. From lblazek <@t> digestivespecialists.com Tue Dec 12 09:39:13 2006 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Dec 12 09:38:10 2006 Subject: [Histonet] Looking for Chris Van der Loos Message-ID: <6CBA6DC98A079D408C87250591D9DFB802684C48@bruexchange.digestivespecialists.com> I got mine on Amazon.com. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Larry Woody Sent: Tuesday, December 12, 2006 10:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Looking for Chris Van der Loos Hi: I tried to order a book ( Immunoenzyme Multiple Staining Methods) by Chris Van Der Loos but my order came back saying out of print and unavailable. Anyone out there know of where we could score a copy of this book? Thanks --------------------------------- Check out the all-new Yahoo! Mail beta - Fire up a more powerful email and get things done faster. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pkarlisch <@t> psu.edu Tue Dec 12 09:43:26 2006 From: pkarlisch <@t> psu.edu (Patricia Karlisch) Date: Tue Dec 12 09:43:58 2006 Subject: [Histonet] Looking for Chris Van der Loos In-Reply-To: <6CBA6DC98A079D408C87250591D9DFB802684C48@bruexchange.digestivespecialists.com> References: <6CBA6DC98A079D408C87250591D9DFB802684C48@bruexchange.digestivespecialists.com> Message-ID: <457E87CE0200008C00032585@GWIA02.HERSHEYMED.NET> You can also get used books on Barnes and Noble. Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. >>> "Blazek, Linda" 12/12/2006 10:39 AM >>> I got mine on Amazon.com. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Larry Woody Sent: Tuesday, December 12, 2006 10:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Looking for Chris Van der Loos Hi: I tried to order a book ( Immunoenzyme Multiple Staining Methods) by Chris Van Der Loos but my order came back saying out of print and unavailable. Anyone out there know of where we could score a copy of this book? Thanks --------------------------------- Check out the all-new Yahoo! Mail beta - Fire up a more powerful email and get things done faster. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Tue Dec 12 09:44:45 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Dec 12 09:44:53 2006 Subject: [Histonet] ALK-1 Controls In-Reply-To: <4D168535-5A2A-4BE6-A18F-83EF43F5C8B3@salk.edu> Message-ID: <281703.13621.qm@web61213.mail.yahoo.com> Any one using umbilical cord membrane as ALK-1 positive control could save a lot of money though! Ren? J. Kim Stecker wrote: Someone asked about ALK-1 controls about two weeks ago and I have some available for $300.00 per block. Kim Stecker The Salk Instutue of Biological Sciences 10010 N. Torrey Pines Rd. La Jolla, CA 92037 Phone: 858-453-4100 X1010 Fax: 858-597-0824 email: stecker@salk.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Want to start your own business? Learn how on Yahoo! Small Business. From lpwenk <@t> sbcglobal.net Tue Dec 12 09:44:39 2006 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Tue Dec 12 09:45:20 2006 Subject: [Histonet] Regarding HT exam & GrandFathering provision In-Reply-To: <41149.89538.qm@web35701.mail.mud.yahoo.com> Message-ID: <002001c71e04$70535380$d2ae2e4b@HPPav2> The new requirements are either: 1. successful completion of a NAACLS accredited histotechnology program (this can be high school graduate through associate degree) OR 2. associate degree or minimum of 60 semester hours, with minimum of 12 hours of biology and chemistry, AND 1 full-time on the job experience. The following is the link to the HT exam requirements http://www.ascp.org/Certification/CertifyingExaminations/cert_procedures/eli gibility/ht.aspx This has been in effect for about 2 years. ASCP and NSH and state histology newsletters had information about the change for at least 5 years pervious to this. There is, and will be, NO grandfathering. Two options for someone with a high school diploma and histology experience to take the exam: 1. Earn the associate degree 2. Contact NAACLS 773-714-8880, and find out which histologic technician programs are accredited for distance learning. I know Indiana University is one that does teleconferences and I know people who have gone through that program. I've also heard that Harford Community College in Maryland is setting up one using the internet. I don't know anyone who has gone through this. Both require that the participants are already working in a histology laboratory, and that their lab will support them with time and resources to do the slides, stains, study and that the exams. It does cost money, so be certain to discuss this with the program officials. (NAACLS = National Accrediting Agency for Clinical Laboratory Sciences) Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Annabel L. Mazza Sent: Monday, December 11, 2006 10:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Regarding HT exam & GrandFathering provision Hi : Does anyone know if the High School diploma/9 years full time histology experience route that is needed to take the HT exam. or will prospective candidates be "grandfathered in?" Thanks in advance for the information. A. Mazza __________________________________________________ Correo Yahoo! Espacio para todos tus mensajes, antivirus y antispam ?gratis! Reg?strate ya - http://correo.espanol.yahoo.com/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TownsendD <@t> childrensdayton.org Tue Dec 12 09:42:17 2006 From: TownsendD <@t> childrensdayton.org (Dolores Townsend) Date: Tue Dec 12 09:50:16 2006 Subject: [Histonet] Looking for Chris Van der Loos Message-ID: You can find copies of it on www.abebooks.com Dolores From cmiller <@t> physlab.com Tue Dec 12 09:54:16 2006 From: cmiller <@t> physlab.com (Cheri Miller) Date: Tue Dec 12 09:54:26 2006 Subject: [Histonet] Regarding HT exam & GrandFathering provision In-Reply-To: <41149.89538.qm@web35701.mail.mud.yahoo.com> Message-ID: <000601c71e05$c7179630$db01a8c0@plab.local> I believe the grandfather route is no longer available. You must have an Associate degree (minimum) and 1 yr work experience before you can sit for the exam. Check the ASCP website. They will have what is required. Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Annabel L. Mazza Sent: Monday, December 11, 2006 9:18 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Regarding HT exam & GrandFathering provision Hi : Does anyone know if the High School diploma/9 years full time histology experience route that is needed to take the HT exam. or will prospective candidates be "grandfathered in?" Thanks in advance for the information. A. Mazza __________________________________________________ Correo Yahoo! Espacio para todos tus mensajes, antivirus y antispam ?gratis! Reg?strate ya - http://correo.espanol.yahoo.com/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JEllin <@t> yumaregional.org Tue Dec 12 09:55:42 2006 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Tue Dec 12 09:55:57 2006 Subject: [Histonet] Grossing Message-ID: <29BE166A2CF48D459853F8EC57CD37E8729E9D@EXCHANGECLUSTER.yumaregional.local> Here at our lab we are haing issues with tech doing gross. Our Pathologist has called the CAP about the ability to do transferance with small biopsies such as GI, Lung, ect. The CAP has stated that this is not considered High complexity testing and that it is ok for the tech to transfer the specimen in a tea bag or what not and then just give measurement and color. Even to use templates for that matter. Nothing is dictated just written down on a piece of paper that is going to be transcribed. Has anyone come up against this situation?? If so what is eveyone doing about this. Also that they do not need to meet the CLIA req about doing gross. To me this opens up a huge can of worms. Any help would greatly be appreciated. Jesus A. Ellin HT ASCP Yuma Regional Medical Center Histology Systems Technologist Pathology Information Systems 928-336-7444 or 928-336-1144 Fax: 928-336-7319 This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. From rjbuesa <@t> yahoo.com Tue Dec 12 10:10:18 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Dec 12 10:10:24 2006 Subject: [Histonet] Grossing In-Reply-To: <29BE166A2CF48D459853F8EC57CD37E8729E9D@EXCHANGECLUSTER.yumaregional.local> Message-ID: <135293.98450.qm@web61225.mail.yahoo.com> Jesus: Regardless of what CLIA may come up with, I think you should consult with your legal department because if by any chance anything done by a histotech regarding grossing ends embroilled in any legal proceedings I think you will be in trouble. Ren? J. Jesus Ellin wrote: Here at our lab we are haing issues with tech doing gross. Our Pathologist has called the CAP about the ability to do transferance with small biopsies such as GI, Lung, ect. The CAP has stated that this is not considered High complexity testing and that it is ok for the tech to transfer the specimen in a tea bag or what not and then just give measurement and color. Even to use templates for that matter. Nothing is dictated just written down on a piece of paper that is going to be transcribed. Has anyone come up against this situation?? If so what is eveyone doing about this. Also that they do not need to meet the CLIA req about doing gross. To me this opens up a huge can of worms. Any help would greatly be appreciated. Jesus A. Ellin HT ASCP Yuma Regional Medical Center Histology Systems Technologist Pathology Information Systems 928-336-7444 or 928-336-1144 Fax: 928-336-7319 This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Need a quick answer? Get one in minutes from people who know. Ask your question on Yahoo! Answers. From gcallis <@t> montana.edu Tue Dec 12 10:35:34 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Dec 12 10:35:52 2006 Subject: [Histonet] Looking for Chris Van der Loos In-Reply-To: <20061212152215.90870.qmail@web53602.mail.yahoo.com> References: <20061212152215.90870.qmail@web53602.mail.yahoo.com> Message-ID: <6.0.0.22.1.20061212092815.01b45b38@gemini.msu.montana.edu> Did you try Amazon.com? At 08:22 AM 12/12/2006, you wrote: >Hi: > I tried to order a book ( Immunoenzyme Multiple Staining Methods) > by Chris Van Der Loos but my order came back saying out of print and > unavailable. Anyone out there know of where we could score a copy of this > book? Thanks > > >--------------------------------- >Check out the all-new Yahoo! Mail beta - Fire up a more powerful email and >get things done faster. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From RSRICHMOND <@t> aol.com Tue Dec 12 11:01:10 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Tue Dec 12 11:01:29 2006 Subject: [Histonet] Re: traveling Mohs tech Message-ID: While we're on the subject: can somebody bring us up to date on how the certification of Mohs techs is progressing? Is there now a formal certification? Is there a Web site about it? Mohs surgery - microsurgical excision of basal cell carcinomas with meticulous frozen section control - is wonderful - my sister had a very successful procedure on an eyelid - is wonderful as long as the surgeon and his assistants prepare and read their own frozen sections. If a pathologist has to do it - two hours tied up with no warning, no idea what the surgeon wants, no communication with the surgeon, wholly inadequate frozen section setup - then Mohs is the worst four letter word a pathologist knows. And I've been through this in a lot of different labs. Bob Richmond Samurai Pathologist Knoxville TN From JMacDonald <@t> mtsac.edu Tue Dec 12 11:06:40 2006 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Dec 12 11:07:04 2006 Subject: [Histonet] Looking for Chris Van der Loos In-Reply-To: <20061212152215.90870.qmail@web53602.mail.yahoo.com> Message-ID: If you take Chris's workshop in the Netherlands you get a copy. :) Larry Woody Sent by: histonet-bounces@lists.utsouthwestern.edu 12/12/2006 07:22 AM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Looking for Chris Van der Loos Hi: I tried to order a book ( Immunoenzyme Multiple Staining Methods) by Chris Van Der Loos but my order came back saying out of print and unavailable. Anyone out there know of where we could score a copy of this book? Thanks --------------------------------- Check out the all-new Yahoo! Mail beta - Fire up a more powerful email and get things done faster. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMacDonald <@t> mtsac.edu Tue Dec 12 11:08:12 2006 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Dec 12 11:08:39 2006 Subject: [Histonet] Cutting and embedding In-Reply-To: Message-ID: I would also be interested in this Jennifer Asf2k3@aol.com Sent by: histonet-bounces@lists.utsouthwestern.edu 12/11/2006 08:45 PM To histonet@lists.utsouthwestern.edu cc Subject [Histonet] Cutting and embedding Does anyone know any good histology websites where they show like a cartoon person cutting on a microtome and embedding, and give advice and hints on such topics. A.S _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Tue Dec 12 11:17:29 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Tue Dec 12 11:17:45 2006 Subject: [Histonet] Re: Floaters Message-ID: <538.2180244d.32b03e29@aol.com> In my previous post on floaters I think I stepped into a problem pathologists commonly handle wrong. In an ordinary morning's sign-out, I'll usually encounter a case or two where the slide's horrible, the diagnosis is obvious, and on to the next case. When I do this, I think I'm being part of the solution, but actually I'm part of the problem. If I may invoke the sacred name of Edwards Deming - Deming emphasized the importance of identifying and addressing small problems before they turn into big problems. If the hematoxylin's a little on the light side today and it isn't changed, the slides may be unreadable tomorrow. That placental villus in the middle of a gastric biopsy - so obvious it may not even rise to my consciousness - may be a floating chunk of squamous carcinoma in the middle of a benign laryngeal biopsy tomorrow. If a junior pathologist complains about an issue of quality or safety, he'll probably get chewed out by the boss for his efforts. I suppose that histotechnologists have the same problem. Shooting the bearer of bad news is a tried and true management technique, probably taught in M.B.A. school right along with Corporate Looting 101 and Advanced Necktie Wearing 203. Using multiple sets of dissecting instruments with the just-used ones soaking in water and washed off every few cases would be a technique worth trying, but I've been ignored when I've brought it up. As for compost, Kemlo, I have quite a large batch of it awaiting my garden next spring. I want to try an Indore heap, but my wife doesn't want it in the living room. Bob Richmond Samurai Pathologist Knoxville TN From PMonfils <@t> Lifespan.org Tue Dec 12 11:41:40 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Tue Dec 12 11:41:54 2006 Subject: [Histonet] Softening chitin - Maria Mejia? Message-ID: <4EBFF65383B74D49995298C4976D1D5E273BD0@LSRIEXCH1.lsmaster.lifespan.org> I'm getting ready for a project on Drosophila brain, and need to section the whole head. Sectioning insects is always difficult because of the chitinous exoskeleton. In the Histonet archives I found a detailed method for addressing this problem, using a mixture of phenol and chloral hydrate, posted by Maria Mejia in 1/06. I intend to use this technique, but would however like to ask a few questions about the technique, of someone who has used it successfully. I have tried emailing Maria, and another person who said he had used the method successfully. But the emails to Maria have bounced back, and I have not yet heard from the other person. If anyone has had success with this method, and would be willing to answer a few brief questions, please email me. Also, if anyone has an up to date email address for Maria Mejia, or if Maria reads this, please email me. Thanks. From JCollins <@t> palmbeachpath.com Tue Dec 12 12:04:13 2006 From: JCollins <@t> palmbeachpath.com (Judy Collins) Date: Tue Dec 12 12:04:23 2006 Subject: [Histonet] Job Opening Message-ID: We are currently seeking a Florida licensed Histotechnician/Histotechnologist for a busy private laboratory in West Palm Beach, Florida. Great pay and benefits. Prefer evening shift or very early AM. Contact: Judy Collins Palm Beach Pathology Phone 561 820-2954 Fax 561 820-2950 E-mail: Jcollins@palmbeachpath.com From tahseen <@t> brain.net.pk Tue Dec 12 12:28:21 2006 From: tahseen <@t> brain.net.pk (Tahseen) Date: Tue Dec 12 12:28:23 2006 Subject: [Histonet] IHC on Apoptolic Cells" (F-7-26) Message-ID: <005601c70688$3145b7c0$101480cb@piii> Is anyone doing immunohistochemical staining for "Apoptolic Cells" (F-7-26) on formalin-fixed, paraffin-embedded tissue? If so, whose antibody are you using? Thanks! Muhammad Tahseen Histology Supervisor, SKMCH&RC Lahore,Pakistan. From tkngflght <@t> yahoo.com Tue Dec 12 12:12:42 2006 From: tkngflght <@t> yahoo.com (Cheryl) Date: Tue Dec 12 13:12:51 2006 Subject: [Histonet] Mohs website In-Reply-To: Message-ID: <20061212181242.37025.qmail@web50915.mail.yahoo.com> There is a website: www.mohscollege.org There are two schools of thought (ways to perform Mohs) and a one-year fellowship program for physicians. You'll find the two different methods can create very vocal responses in those trained to do this work. This website is a great resource and includes discussion on certification for techs. Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one Tech at a time. 281.883.7704 c 281.852.9457 o admin@fullstaff.org Sign up for the FREE monthly newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. From histology <@t> gradymem.org Tue Dec 12 14:21:16 2006 From: histology <@t> gradymem.org (histology@gradymem.org) Date: Tue Dec 12 14:21:25 2006 Subject: [Histonet] Grossing In-Reply-To: <29BE166A2CF48D459853F8EC57CD37E8729E9D@EXCHANGECLUSTER.yumaregional.local> References: <29BE166A2CF48D459853F8EC57CD37E8729E9D@EXCHANGECLUSTER.yumaregional.local> Message-ID: I was told by a CLIA person in OKC that if the description becomes part of the parthology report that it is complex testing and must be CLIA qualified. Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org ----- Original Message ----- From: Jesus Ellin Date: Tuesday, December 12, 2006 10:06 am Subject: [Histonet] Grossing To: histonet@lists.utsouthwestern.edu > Here at our lab we are haing issues with tech doing gross. Our > Pathologist has called the CAP about the ability to do > transferance with > small biopsies such as GI, Lung, ect. The CAP has stated that > this is > not considered High complexity testing and that it is ok for the > tech to > transfer the specimen in a tea bag or what not and then just give > measurement and color. Even to use templates for that matter. > Nothingis dictated just written down on a piece of paper that is > going to be > transcribed. Has anyone come up against this situation?? If so > what is > eveyone doing about this. Also that they do not need to meet the CLIA > req about doing gross. > > To me this opens up a huge can of worms. Any help would greatly be > appreciated. > > Jesus A. Ellin HT ASCP > Yuma Regional Medical Center > Histology Systems Technologist > Pathology Information Systems > 928-336-7444 or 928-336-1144 > Fax: 928-336-7319 > > > > > This message is confidential, intended only for the named > recipient(s) and may contain information that is privileged > or exempt from disclosure under applicable law. If you are > not the intended recipient(s), you are notified that the > dissemination, distribution, or copying of this message is > strictly prohibited. If you receive this message in error, > or are not the named recipient(s), please notify the sender > at either the e-mail, fax, address, or telephone number > listed above and delete this e-mail from your computer. > Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From LRaff <@t> lab.uropartners.com Tue Dec 12 15:46:11 2006 From: LRaff <@t> lab.uropartners.com (Lester Raff) Date: Tue Dec 12 15:46:32 2006 Subject: [Histonet] Grossing Message-ID: <5DA1CA5D0B98A84985B545A24423B822019B23@UPLAB01.uplab.local> We are a lab only processing biopsies. We hired a person who met the CLIA regs for education with a degree in Biological Science and the required number of science courses (see below). We then developed an in house 18 week training program with pre and post test, documentation, supervision, etc. We specify in our procedures, and in the lab assistants credentials, exactly what specimens she can gross. We had no problems at our CAP inspection. Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 NOTE: The laboratory director may delegate the dissection of specimens to non-pathologist individuals; these individuals must be qualified as high complexity testing personnel under CLIA-88 regulations. The minimum training/experience required of such personnel is: 1. An earned associate degree in a laboratory science or medical laboratory technology, obtained from an accredited institution, OR 2. Education/training equivalent to the above that includes at least 60 semester hours or equivalent from an accredited institution. This education must include 24 semester hours of medical laboratory technology courses, OR 24 semester hours of science courses that includes 6 semester hours of chemistry, 6 semester hours of biology, and 12 semester hours of chemistry, biology or medical laboratory technology in any combination. In addition, the individual must have laboratory training including either completion of a clinical laboratory training program approved or accredited by the ABHES, the CAHEA, or other organization approved by HHS (note that this training may be included in the 60 semester hours listed above), OR at least 3 months documented laboratory training in each specialty in which the individual performs high complexity testing. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histology@gradymem.org Sent: Tuesday, December 12, 2006 2:21 PM To: Jesus Ellin Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Grossing I was told by a CLIA person in OKC that if the description becomes part of the parthology report that it is complex testing and must be CLIA qualified. Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org ----- Original Message ----- From: Jesus Ellin Date: Tuesday, December 12, 2006 10:06 am Subject: [Histonet] Grossing To: histonet@lists.utsouthwestern.edu > Here at our lab we are haing issues with tech doing gross. Our > Pathologist has called the CAP about the ability to do > transferance with > small biopsies such as GI, Lung, ect. The CAP has stated that > this is > not considered High complexity testing and that it is ok for the > tech to > transfer the specimen in a tea bag or what not and then just give > measurement and color. Even to use templates for that matter. > Nothingis dictated just written down on a piece of paper that is > going to be > transcribed. Has anyone come up against this situation?? If so > what is > eveyone doing about this. Also that they do not need to meet the CLIA > req about doing gross. > > To me this opens up a huge can of worms. Any help would greatly be > appreciated. > > Jesus A. Ellin HT ASCP > Yuma Regional Medical Center > Histology Systems Technologist > Pathology Information Systems > 928-336-7444 or 928-336-1144 > Fax: 928-336-7319 > > > > > This message is confidential, intended only for the named > recipient(s) and may contain information that is privileged > or exempt from disclosure under applicable law. If you are > not the intended recipient(s), you are notified that the > dissemination, distribution, or copying of this message is > strictly prohibited. If you receive this message in error, > or are not the named recipient(s), please notify the sender > at either the e-mail, fax, address, or telephone number > listed above and delete this e-mail from your computer. > Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Tue Dec 12 15:55:05 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Tue Dec 12 15:55:15 2006 Subject: [Histonet] Grossing References: <29BE166A2CF48D459853F8EC57CD37E8729E9D@EXCHANGECLUSTER.yumaregional.local> Message-ID: <000b01c71e38$2fb32560$be604542@yourxhtr8hvc4p> one of my pathologist's is on the board at CAP and we have discussed this many times. In fact, earlier this summer, he did address the AAPA group. As far as I know, CAP is having a discussion on what is "grossing" and what is "dictating". As far as "complex" testing, if your lab is qualified as "complex testing" then complex testing is okayed. The issue may come up when the techs do not have any college hours. This again is questionable because techs performing IHC are capable of performing complex tests. Their results are reported on a path report (but the doctors are rendering an interpretation). Same thing with specials. If I perform a GMS, the result is issued in the report. So, my conclusion is that this is one of those huge gray areas that have not been definitely defined yet. In my lab, a have a couple of techs that have anywhere between 30-50 college hours and they are grossing. Just make sure that the medical director knows the situation and is directly or indirectly supervising the grossing. OK, is anyone as confused as I am? Gee, looks like I just made things more complex. Just like the old days. Joe Nocito ----- Original Message ----- From: To: "Jesus Ellin" Cc: Sent: Tuesday, December 12, 2006 2:21 PM Subject: Re: [Histonet] Grossing >I was told by a CLIA person in OKC that if the description becomes part of >the parthology report that it is complex testing and must be CLIA >qualified. > > Angie Barnett, HTL(ASCP) > Grady Memorial Hospital > Pathology Department > 405/224-2258 > histology@gradymem.org > > > ----- Original Message ----- > From: Jesus Ellin > Date: Tuesday, December 12, 2006 10:06 am > Subject: [Histonet] Grossing > To: histonet@lists.utsouthwestern.edu > >> Here at our lab we are haing issues with tech doing gross. Our >> Pathologist has called the CAP about the ability to do >> transferance with >> small biopsies such as GI, Lung, ect. The CAP has stated that >> this is >> not considered High complexity testing and that it is ok for the >> tech to >> transfer the specimen in a tea bag or what not and then just give >> measurement and color. Even to use templates for that matter. >> Nothingis dictated just written down on a piece of paper that is >> going to be >> transcribed. Has anyone come up against this situation?? If so >> what is >> eveyone doing about this. Also that they do not need to meet the CLIA >> req about doing gross. >> >> To me this opens up a huge can of worms. Any help would greatly be >> appreciated. >> >> Jesus A. Ellin HT ASCP >> Yuma Regional Medical Center >> Histology Systems Technologist >> Pathology Information Systems >> 928-336-7444 or 928-336-1144 >> Fax: 928-336-7319 >> >> >> >> >> This message is confidential, intended only for the named >> recipient(s) and may contain information that is privileged >> or exempt from disclosure under applicable law. If you are >> not the intended recipient(s), you are notified that the >> dissemination, distribution, or copying of this message is >> strictly prohibited. If you receive this message in error, >> or are not the named recipient(s), please notify the sender >> at either the e-mail, fax, address, or telephone number >> listed above and delete this e-mail from your computer. >> Thank you. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie <@t> conxis.com Tue Dec 12 19:23:40 2006 From: laurie <@t> conxis.com (Laurie A. Popp) Date: Tue Dec 12 19:24:09 2006 Subject: [Histonet] RE: Histonet Digest, Vol 37, Issue 15 Message-ID: <001601c71e55$52d6c9d0$9700a8c0@Laurie> UND has been working with Mayo to set one up and has just had it's NAACLS inspection. All didactic work is done online and combined with clinical time in the lab that you're working in. Laurie Popp HT Candidate Message: 8 Date: Tue, 12 Dec 2006 10:44:39 -0500 From: "Lee & Peggy Wenk" Subject: RE: [Histonet] Regarding HT exam & GrandFathering provision To: "'Annabel L. Mazza'" , Message-ID: <002001c71e04$70535380$d2ae2e4b@HPPav2> Content-Type: text/plain; charset="iso-8859-1" The new requirements are either: 1. successful completion of a NAACLS accredited histotechnology program (this can be high school graduate through associate degree) OR 2. associate degree or minimum of 60 semester hours, with minimum of 12 hours of biology and chemistry, AND 1 full-time on the job experience. The following is the link to the HT exam requirements http://www.ascp.org/Certification/CertifyingExaminations/cert_procedures/eli gibility/ht.aspx This has been in effect for about 2 years. ASCP and NSH and state histology newsletters had information about the change for at least 5 years pervious to this. There is, and will be, NO grandfathering. Two options for someone with a high school diploma and histology experience to take the exam: 1. Earn the associate degree 2. Contact NAACLS 773-714-8880, and find out which histologic technician programs are accredited for distance learning. I know Indiana University is one that does teleconferences and I know people who have gone through that program. I've also heard that Harford Community College in Maryland is setting up one using the internet. I don't know anyone who has gone through this. Both require that the participants are already working in a histology laboratory, and that their lab will support them with time and resources to do the slides, stains, study and that the exams. It does cost money, so be certain to discuss this with the program officials. (NAACLS = National Accrediting Agency for Clinical Laboratory Sciences) Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -- No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.5.432 / Virus Database: 268.15.16/582 - Release Date: 12/11/2006 4:32 PM From Kemlo.Rogerson <@t> waht.swest.nhs.uk Wed Dec 13 02:12:05 2006 From: Kemlo.Rogerson <@t> waht.swest.nhs.uk (Kemlo Rogerson) Date: Wed Dec 13 02:10:58 2006 Subject: [Histonet] Re: Floaters Message-ID: <86ADE4EB583CE64799A9924684A0FBBF01246FC3@wahtntex2.waht.swest.nhs.uk> I agree with your sentiments and things, like compost, ought not to be allowed to rest. Errors occur when the opportunities line up and the error falls through the holes; close the gaps and stop the rare but significant event. Kemlo Rogerson Shotokan Pathology Manager Ext 3311 DD 01934 647057 Mob 07749 754194 Pager 07659 597107 E-Mail: kemlo@f2s.com Prosperity is a way of living and thinking, and not just money or things. Poverty is a way of living and thinking, and not just a lack of money or things. --Eric Butterworth This e-mail is confidential and privileged. If you are not the intended recipient please accept my apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform me that this message has gone astray before deleting it. Thank you for your co-operation From David.Muskett <@t> elht.nhs.uk Wed Dec 13 08:41:07 2006 From: David.Muskett <@t> elht.nhs.uk (Muskett David (ELHT) Pathology) Date: Wed Dec 13 08:41:17 2006 Subject: [Histonet] Audit trails for specimens moving through the laboratory Message-ID: <53A22BBB01D6184EBF7A4DC18A021E9E04A52677@elht-exch1.xelht.nhs.uk> Dear All I am currently reviewing the specimen tracking procedures within our laboratory. Can any one advise to methods they use within their laboratory about Tracking the number of pieces within the lab Tracking who has embedded the tissue. Any advice or SOPs are welcome. Regards David David Muskett Chief Biomedical Scientist, Histology East Lancashire Hospitals NHS Trust, UK From ergonomics <@t> ehs.unc.edu Wed Dec 13 09:13:29 2006 From: ergonomics <@t> ehs.unc.edu (Bertmaring, Ian (Environment Health & Safety)) Date: Wed Dec 13 09:13:38 2006 Subject: [Histonet] Grossing postures and procedures - Ergonomics In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E273BCF@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <79ACF88C02E12F419FE8ECA36001D8FAA3329E@auxsexc1.aux-services.unc.edu> Thanks for all the help, everyone. So far I have 2 ideas, run with them if you'd like for your situation, or comment as well. Periscope Idea, DIY -Except only 1 turn, creates problem of magnification and orientation (mirror reverses image) -Solution, create tube with several (odd in number) convex lenses, and a mirror at 45 degrees at the top over the specimen. -Will magnify and allow work in a neutral posture (working at elbow height with hands and eyes looking forward to the mirror. Camera Idea -expensive alternative -project image pf specimen on a screen at eye level. Both are simple add-ons that allow the inking, sectioning, etc. to be performed on a horizontal surface but also reduce the risk of neck and back injury. Currently looking into building the "periscope." BTW does anyone know if this thing has a name besides calling it a modified microscope. Thanks, Ian Ian Bertmaring, MS, AEP - Ergonomist Department of Environment, Health & Safety 1120 Estes Drive Extension, CB #1650 Chapel Hill, NC 27599 - 1650 Phone: (919) 843 - 4642; Fax: (919) 962 - 0227 ergonomics@ehs.unc.edu http://www.ehs.unc.edu/workplace_safety/ergonomics/ -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Monday, December 11, 2006 5:07 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Grossing postures and proceedures - Ergonomics Possibly an angled mirror over the specimen? Bottom edge of the mirror in contact with the benchtop, behind the specimen; mirror leaning toward the tech at a suitable angle? This would give the tech a perpendicular view of the specimen with a normal angle of view for the tech. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bertmaring,Ian (Environment Health & Safety) > Sent: Monday, December 11, 2006 1:46 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Grossing postures and proceedures - Ergonomics > > I have a situation that I am looking for a solution for and I figured > some one in this community could help me. > > The Problem: > When techs at UNC perform grossing, they often lean (while standing or > seated) with their head into the fume hood/bio safety cabinet to get a > top down view of the biopsies and excisions (performing inking, > describing markers, and slicing samples). This posture poses a > significant risk of injury on the back and neck thus the need for a > workstation adjustment. The risk of inhalation is low, but present, > so that is a minor concern as well. > > Initial Thoughts: > 1. Rotate the sample to face the histologist, like reading a book. > 2. Affix the sample to a cutting board (or the surface currently in > use) by means of a reverse action tweezers mounted to the board. > 3. At this angle the histotech can see the sample with minimal neck > bending (while keeping the head out of the hood) as well as reduce the > need to hold the sample while performing inking, etc. > > Questions: > 1. Has anyone tried this? > 2. Does anyone else have a solution that would work in this situation? > 3. What is your opinion on this solution? > > Any ideas or comments are greatly welcome. > > Thank you! > > Ian > > > Ian Bertmaring, MS, AEP - Ergonomist > Department of Environment, Health & Safety 1120 Estes Drive Extension, > CB #1650 Chapel Hill, NC 27599 - 1650 > > Phone: (919) 843 - 4642; Fax: (919) 962 - 0227 > > ergonomics@ehs.unc.edu > > http://www.ehs.unc.edu/workplace_safety/ergonomics/ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Dec 13 09:26:46 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Dec 13 09:26:59 2006 Subject: [Histonet] Audit trails for specimens moving through the laboratory In-Reply-To: <53A22BBB01D6184EBF7A4DC18A021E9E04A52677@elht-exch1.xelht.nhs.uk> Message-ID: <844019.28091.qm@web61223.mail.yahoo.com> David: I used to have a log where a sticker with the case number was attached with space to write down the number and code of the cassettes used for each case, and the number of pieces in the cassette. The HT doing cassetiing wrote his/hers initials for each case. The next day that same log was used to check the cassette contents when embedding. The log was also initialized (by page, not by case this time) by the HT doing the embedding. This same log was used to assign cassettes to cut, and the initials of the HT sectioning was also added. In this way one single log was initialized at the grossing station, and was used during cassetting, embedding and to record the HT sectioning. Later on it was filed in 3 ring binders if somebody wanted to know who intervened in the different steps in any specific case. The information was used also to determine productivity during the different steps. Hope this will help you! Ren? J. "Muskett David (ELHT) Pathology" wrote: Dear All I am currently reviewing the specimen tracking procedures within our laboratory. Can any one advise to methods they use within their laboratory about Tracking the number of pieces within the lab Tracking who has embedded the tissue. Any advice or SOPs are welcome. Regards David David Muskett Chief Biomedical Scientist, Histology East Lancashire Hospitals NHS Trust, UK _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Everyone is raving about the all-new Yahoo! Mail beta. From bwhitaker <@t> brownpathology.com Wed Dec 13 09:54:20 2006 From: bwhitaker <@t> brownpathology.com (Bonnie Whitaker) Date: Wed Dec 13 09:50:12 2006 Subject: [Histonet] Grossing In-Reply-To: Message-ID: <004501c71ece$f385d8c0$3601a8c0@brownpathology.net> That's what a former CLIA inspector told me, too. Also, if you are CAP, you have deemed status by CLIA, but they still COULD come in and inspect you!! You have to follow any local, state or national laws that are applicable to you, as well as being CAP certified. Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histology@gradymem.org Sent: Tuesday, December 12, 2006 2:21 PM To: Jesus Ellin Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Grossing I was told by a CLIA person in OKC that if the description becomes part of the parthology report that it is complex testing and must be CLIA qualified. Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org ----- Original Message ----- From: Jesus Ellin Date: Tuesday, December 12, 2006 10:06 am Subject: [Histonet] Grossing To: histonet@lists.utsouthwestern.edu > Here at our lab we are haing issues with tech doing gross. Our > Pathologist has called the CAP about the ability to do > transferance with > small biopsies such as GI, Lung, ect. The CAP has stated that > this is > not considered High complexity testing and that it is ok for the > tech to > transfer the specimen in a tea bag or what not and then just give > measurement and color. Even to use templates for that matter. > Nothingis dictated just written down on a piece of paper that is > going to be > transcribed. Has anyone come up against this situation?? If so > what is > eveyone doing about this. Also that they do not need to meet the CLIA > req about doing gross. > > To me this opens up a huge can of worms. Any help would greatly be > appreciated. > > Jesus A. Ellin HT ASCP > Yuma Regional Medical Center > Histology Systems Technologist > Pathology Information Systems > 928-336-7444 or 928-336-1144 > Fax: 928-336-7319 > > > > > This message is confidential, intended only for the named > recipient(s) and may contain information that is privileged > or exempt from disclosure under applicable law. If you are > not the intended recipient(s), you are notified that the > dissemination, distribution, or copying of this message is > strictly prohibited. If you receive this message in error, > or are not the named recipient(s), please notify the sender > at either the e-mail, fax, address, or telephone number > listed above and delete this e-mail from your computer. > Thank you. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jacqueline.Malam <@t> rli.mbht.nhs.uk Wed Dec 13 09:48:44 2006 From: Jacqueline.Malam <@t> rli.mbht.nhs.uk (Malam Jacqueline) Date: Wed Dec 13 09:53:24 2006 Subject: [Histonet] RE: Endogenous biotin ?? Message-ID: I am trying to help someone from another hospital who is having immuno problems with non-specific staining in some of their negative controls when using a Ventana Benchmark. I also got the same staining using their slides on my Benchmark XT. It can appear in mucin-producing cells of e.g. gastric tissue, lymphoid tissues and rather spectacularly in Leydig cells in testis, but it does not appear in the same type of tissues all the time nor does it appear when they do manual antigen retrieval and use their Ventana Nexus. The staining pattern has the appearance of endogenous biotin and testis does indeed contain biotin; but I would have thought that any endogenous biotin in the tissues would have shown up on the Nexus-stained slides which had manual antigen retrieval. I am wondering if there is some neuro-endocrine involvement rather than biotin. Ventana have been extremely helpful but are also scratching their heads too! The only significant difference between the two methods is the fact that manual antigen retrieval is performed at a much higher temperature and for a shorter time than the Benchmark. Fixation, processing, reagents and equipment malfunction has supposedly been ruled out. Your help and comments would be much appreciated by us both. Jacqui DISCLAIMER: This e-mail is confidential and privileged. If you are not the intended recipient please accept our apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform postmaster@rli.mbht.nhs.uk that this message has gone astray before deleting it. Comments or opinions expressed in this email are those of their respective contributors only. The views expressed do not represent the views of the Trust, its management or employees. University Hospitals of Morecambe Bay NHS Trust is not responsible and disclaims any and all liability for the content of comments written within.Thank you for your co-operation. From mprice26 <@t> juno.com Wed Dec 13 10:38:21 2006 From: mprice26 <@t> juno.com (mprice26@juno.com) Date: Wed Dec 13 10:39:29 2006 Subject: [Histonet] Re: Block used for Polymer detection System Message-ID: <20061213.083859.10246.901965@webmail30.nyc.untd.com> Histonetters, Can someone share their recipe for the block used for the Polymer Detection System? I know you are not supposed to have to worry about endogenous biotin any longer with this detection system, however, I have had problems with endogenous biotin with certain types of tissue such as bronchial. I have been using only a peroxide block as is suggested by the manufacturer. Thank you. Marsha Price From slappycraw <@t> yahoo.com Wed Dec 13 10:50:19 2006 From: slappycraw <@t> yahoo.com (Larry Woody) Date: Wed Dec 13 10:50:33 2006 Subject: [Histonet] Re: Block used for Polymer detection System In-Reply-To: <20061213.083859.10246.901965@webmail30.nyc.untd.com> Message-ID: <29334.68115.qm@web53609.mail.yahoo.com> You can throw in a protein block if you want, we usually use casein. "mprice26@juno.com" wrote: Histonetters, Can someone share their recipe for the block used for the Polymer Detection System? I know you are not supposed to have to worry about endogenous biotin any longer with this detection system, however, I have had problems with endogenous biotin with certain types of tissue such as bronchial. I have been using only a peroxide block as is suggested by the manufacturer. Thank you. Marsha Price _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Access over 1 million songs - Yahoo! Music Unlimited. From hej01 <@t> health.state.ny.us Wed Dec 13 10:49:50 2006 From: hej01 <@t> health.state.ny.us (Helen E Johnson) Date: Wed Dec 13 10:52:03 2006 Subject: [Histonet] mosquito section on floatation bath Message-ID: Hi Histonetters, I am having a problem when I place FFPE mosquito sections on the floatation bath. The abdomen explodes. This is happening only with experimentally infected mosquitoes and not with noninfected mosquitoes. I tried lowering the temperature of the floatation bath. Any suggestions? Helen Johnson (hej01@health.state.ny.us) From PMonfils <@t> Lifespan.org Wed Dec 13 11:15:57 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Dec 13 11:16:06 2006 Subject: [Histonet] mosquito section on floatation bath In-Reply-To: Message-ID: <4EBFF65383B74D49995298C4976D1D5E273BD5@LSRIEXCH1.lsmaster.lifespan.org> While I can't explain the difference between the infected and non-infected mosquitos, such exposion usually indicates poor infiltration with paraffin - which would also be hard to explain if all the specimens were processed together. In any case, this problem can often be avoided by adding a small drop of liquid dish detergent to the water bath to reduce surface tension. (detergent made for hand washing of dishes, not dishwasher detergent) > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Helen E Johnson > Sent: Wednesday, December 13, 2006 8:49 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] mosquito section on floatation bath > > > Hi Histonetters, > I am having a problem when I place FFPE mosquito sections on the > floatation bath. The abdomen explodes. This is happening only with > experimentally infected mosquitoes and not with noninfected mosquitoes. I > tried lowering the temperature of the floatation bath. Any suggestions? > Helen Johnson (hej01@health.state.ny.us) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From Charles.Embrey <@t> carle.com Wed Dec 13 11:43:24 2006 From: Charles.Embrey <@t> carle.com (Charles.Embrey) Date: Wed Dec 13 11:44:06 2006 Subject: [Histonet] Grossing Message-ID: <44780C571F28624DBB446DE55C4D733A1FE491@EXCHANGEBE1.carle.com> The standards are very clear and leave no room for a grey area but the problem is that there are two standards. CAP, which is an organization run by pathologists, for pathologists, downgraded federal standards to meet their own agenda. The CLIA '88 standard is not all that high either. You do not have to be a pathologist or PA to gross but you must meet standards as high complexity testing personnel. College credit about equal to an associate's degree with certain hours towards biology and chemistry will get you there. Now the idea of proper compensation is a whole new ball of wax. The reason many pathologists hire folks other than PA's to gross is to save money and will try to get by paying as little as possible. As long as there a people willing to do it for lab assistant pay or less, the practice will continue. Charles Embrey, PA(ASCP) -----Original Message----- From: Jesus Ellin [mailto:JEllin@yumaregional.org] Sent: Wednesday, December 13, 2006 9:30 AM To: Charles.Embrey Cc: Histonet@pathology.swmed.edu Subject: RE: [Histonet] Grossing The problem comes down to, that there needs to be a black and white answer so that there is no room for gray. From speaking with other techs and professionals that including Pathologists, this is done to help alleviate the stress of the pathologists having to do gross. But there are not enough Pathologists or Pathologists Assistants to do the job. IF this is going to be a new area that is being explored for Histo techs to do, how do we come up with a compensation for this type of work?? What is everyone else's feeling towards this matter? Are any other labs seeing this trend take place?? Jesus A. Ellin HT ASCP Yuma Regional Medical Center Histology Systems Technologist Pathology Information Systems 928-336-7444 or 928-336-1144 Fax: 928-336-7319 This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. From Jessica <@t> medstaffservices.com Wed Dec 13 13:08:40 2006 From: Jessica <@t> medstaffservices.com (Jessica Hirsch) Date: Wed Dec 13 13:08:56 2006 Subject: [Histonet] Career Opportunities in Histology Field Message-ID: CURRENT EMPLOYMENT OPPROTUNITIES ( HISTOLOGY, IMMUNOHISTOCHEMISTRY & PATHOLOGY ) I. Clarksburg, Maryland (Large Nationwide Clinical Labratory (division of BioReference)) 1. Histology Supervisor: Hours: Monday - Friday 12 midnight - 8:30am Pay: $25/hr + 10% night differential = $27.50/hr Benefits: Medical, Dental, 401k 2. Histology Technologist Hours: Monday - Friday 12 midnight - 8:30am Pay: $25/hr + 10% night differential = $27.50/hr Benefits: Medical, Dental, 401k 3. Grossers Hours: Monday - Friday 7pm-11:30pm Pay: $30/hr II. Elmwood Park, New Jersey ( Nationwide Clinical Laboratory) 1. Histology Technologist ( 2 positions available, immediate start ) Hours: Monday - Friday, 9:00pm - 5:30am Pay: Negotiable Benefits: Medical, Dental, 401k 2. ImmunoHistoChemistry Supervisor Hours: Monday - Friday, 7am or 8am - 4pm or 5pm Pay: Negotiable Benefits: Medical, Dental, 401k III. New York City, New York ( large world renowned Cancer Hospital ) 1. Pathologist Assistant Hours: Monday - Friday, Day shift, Full-Time Pay: $60k - $80k based on experience Benefits: Major Medical, Dental, Vision, 401k, etc. 2. Histology Technologist Hours: Monday - Friday (shift to be announced) Pay: negotiable Benefits: Medical, Dental, 401k For immediate consideration for any position: Send resumes to Jessica@medstaffservices.com OR fax to 732.238.2152 For questions call: Jessica Hirsch at 732.238.6050 ext.58 Jessica D. Hirsch Staffing Specialist Medical Staffing Services, Inc. 557 Cranbury Road East Brunswick, NJ 08816 P: (732) 238-6050 x58 F: (732) 238-2152 Jessica@medstaffservices.com http://www.medstaffservices.com From katri <@t> cogeco.ca Wed Dec 13 13:50:02 2006 From: katri <@t> cogeco.ca (Katri Tuomala) Date: Wed Dec 13 13:50:07 2006 Subject: [Histonet] RE: Endogenous biotin ?? References: Message-ID: <002301c71eef$e0fc0be0$6a9a9618@Katri> Jacqui, Have you ruled out the endogenous biotin by repeating the same test slide with Biotin block? We tackle with endogenous biotin almost daily. Many types of cells have it in varying amounts. The reason we are seeing it now so often is to do with various heat retrievals combined with short fixation times. We hardly ever had this problem before HIER, formalin fixation masked most of it, if it was present. Now we often end up repeating cases with a polymer detection system, which does not use avidin. Unfortunately it is still too expensive for us to use routinely, but that maybe the only route to go in the future. But then, the polymers may have their own problems. We are now trying integrating the biotin block into the primary antibody diluents and the detection system to eliminate the extra steps you need to do. In your case the Ventana retrieval must be somehow stronger , than the manual one, therefore revealing the biotin present. Unfortunately one can never predict which case will have endogenous biotin. The amount varies in tissues of all kinds and many tumors have an abundant amount, sometimes even too much to block with a biotin block. This problem will not be easily solved. If somebody has some brilliant ideas, pass them my way too... Katri Katri Tuomala Hamilton, Ontario, Canada ----- Original Message ----- From: "Malam Jacqueline" To: Sent: Wednesday, December 13, 2006 10:48 AM Subject: [Histonet] RE: Endogenous biotin ?? >I am trying to help someone from another hospital who is having immuno > problems with non-specific staining in some of their negative controls > when > using a Ventana Benchmark. I also got the same staining using their slides > on my Benchmark XT. It can appear in mucin-producing cells of e.g. gastric > tissue, lymphoid tissues and rather spectacularly in Leydig cells in > testis, > but it does not appear in the same type of tissues all the time nor does > it > appear when they do manual antigen retrieval and use their Ventana Nexus. > The staining pattern has the appearance of endogenous biotin and testis > does > indeed contain biotin; but I would have thought that any endogenous biotin > in the tissues would have shown up on the Nexus-stained slides which had > manual antigen retrieval. I am wondering if there is some neuro-endocrine > involvement rather than biotin. Ventana have been extremely helpful but > are > also scratching their heads too! The only significant difference between > the > two methods is the fact that manual antigen retrieval is performed at a > much > higher temperature and for a shorter time than the Benchmark. Fixation, > processing, reagents and equipment malfunction has supposedly been ruled > out. > Your help and comments would be much appreciated by us both. > > Jacqui > > > > DISCLAIMER: This e-mail is confidential and privileged. If you are not the > intended recipient please accept our apologies; please do not disclose, > copy > or distribute information in this e-mail or take any action in reliance on > its contents: to do so is strictly prohibited and may be unlawful. Please > inform postmaster@rli.mbht.nhs.uk that this message has gone astray before > deleting it. Comments or opinions expressed in this email are those of > their respective contributors only. The views expressed do not represent > the > views of the Trust, its management or employees. University Hospitals of > Morecambe Bay NHS Trust is not responsible and disclaims any and all > liability for the content of comments written within.Thank you for your > co-operation. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From LRaff <@t> lab.uropartners.com Wed Dec 13 14:02:04 2006 From: LRaff <@t> lab.uropartners.com (Lester Raff) Date: Wed Dec 13 14:02:17 2006 Subject: [Histonet] Better Nucleoli Message-ID: <5DA1CA5D0B98A84985B545A24423B822019B26@UPLAB01.uplab.local> Hello 'net We have nice, thinly cut, well stained prostate biopsies (fomalin fixed, stained on Sakura Tissue Tech with a 3 hour protocol). However, we are having trouble seeing prominent nucleoli. Any suggestions? Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 From contact <@t> excaliburpathology.com Wed Dec 13 14:30:23 2006 From: contact <@t> excaliburpathology.com (P Pierce) Date: Wed Dec 13 14:30:34 2006 Subject: [Histonet] mosquito sections on floatation bath Message-ID: <20061213203023.97841.qmail@web50114.mail.yahoo.com> Were the infected mosquitos fed before being sacrificed and not the non-infected ones? Could be stomach contents are not thoroughly fixed and/or infiltrated. Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-570-6679 405-759-3953 contact@excaliburpathology.com From Rcartun <@t> harthosp.org Wed Dec 13 14:48:41 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Dec 13 14:48:55 2006 Subject: [Histonet] Requests for extra slides Message-ID: <458020D90200007700003462@hcnwgwds01.hh.chs> Our Anatomic Pathology Division is overwhelmed with requests for the preparation of H&E slides as well as unstained slides for clinical trails, research protocols, etc., etc., etc. How are other institutions handling these requests? We barely have enough manpower now to do our routine daily work. Is anyone charging for this and, if so, how much? Thanks. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From godsgalnow <@t> aol.com Wed Dec 13 15:01:59 2006 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Wed Dec 13 15:02:15 2006 Subject: [Histonet] Better Nucleoli In-Reply-To: <5DA1CA5D0B98A84985B545A24423B822019B26@UPLAB01.uplab.local> References: <5DA1CA5D0B98A84985B545A24423B822019B26@UPLAB01.uplab.local> Message-ID: <8C8ED11106D1204-11AC-6948@FWM-D28.sysops.aol.com> You are staining for 3 hours....do you mean processing? What stains are you usning? We do mostly urology specimens here as well. We use Richard Allen and get excellent results. What is your protocol? Roxanne Soto HT(ASCP)QIHC Lab Manager Physicians RightPath.com Tampa, Florida -----Original Message----- From: LRaff@lab.uropartners.com To: histonet@lists.utsouthwestern.edu Sent: Wed, 13 Dec 2006 3:02 PM Subject: [Histonet] Better Nucleoli Hello 'net We have nice, thinly cut, well stained prostate biopsies (fomalin fixed, stained on Sakura Tissue Tech with a 3 hour protocol). However, we are having trouble seeing prominent nucleoli. Any suggestions? Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From Steven2146 <@t> aol.com Wed Dec 13 18:29:21 2006 From: Steven2146 <@t> aol.com (Steven2146@aol.com) Date: Wed Dec 13 18:29:31 2006 Subject: [Histonet] Re: Histonet Digest, Vol 37, Issue 16 Message-ID: There is also another site that places Mohs techs and also trains them... www.mohstechstaffing.com From WWmn916 <@t> aol.com Wed Dec 13 21:41:19 2006 From: WWmn916 <@t> aol.com (WWmn916@aol.com) Date: Wed Dec 13 21:41:31 2006 Subject: [Histonet] Histoprep bx bags? Message-ID: <279.21f9be9d.32b221df@aol.com> Can any tell me why the Histoprep biopsy bags have been discontinued? The replacement bags are nylon and not what works for our lab. Has anyone found an exceptable replacement other than sewn nylon bags? Thanks, Deb in Sacramento From debbiekeith <@t> cox.net Wed Dec 13 22:12:11 2006 From: debbiekeith <@t> cox.net (Debbie Keith) Date: Wed Dec 13 22:12:21 2006 Subject: [Histonet] cryostat... part DEAUX Message-ID: <5.2.0.9.0.20061213204318.03051318@pop.central.cox.net> hello all! for those of you that read the written saga of my cryostat woes... i thought i would update you. (actually, it's sort of like therapy. getting it out is like writing "the letter" that you never send to your mother-in-law.) i'm sure some of you KNOW what i mean. ;) i went in on Saturday to face an add-on day of surgeries that we had to cancel during the week (because of cryostat issues). i KNEW the machine was "challenged", but i was armed with 2 things. ONE, i picked up some anti-vibration lubricant (tip from Don at Leica!) AND Barry from Belair was over-nighting the part that was the source of my angst. surgery started at 7:30am... and the part was there by 9am!! (Barry ROCKS!) i would like everyone to know that installing that tiny pair of parts was VERY tricky, indeed! it required Herculean effort, a hand mirror and positions i've only seen at cirque de soleil. in a different setting... it might sound like fun. not in the lab. :) after installing the bits... there was a remarkable change. alas, i have realized that my problem was multi-faceted. here is my theory... (i post this here... hoping someone might provide input to support or to the contrary..) before installation of the half-moon-shaped parts inside the chuck assembly (generously and expeditiously provided by Barry at Belair!).... it was totally unstable. the chuck was never tight and given any challenge the machine would skip/gouge/chatter. for a while it seemed fine... actually, it was GREAT! (it even made THE sound!) after a while it started the thick/thin thing... and that turned into chattering. i decided to turn the thickness down, hoping that my "thick" section would be at least acceptable. i turned the dial to 4 microns... and the machine started cutting perfectly... even making THE sound. no thick/thin... just perfect 4 micron sections every time! interesting!? a few blocks later... it started again. i "fixed" it by simply turning the dial from 4 to 50 and back again. tell me how odd that is!?!?!? here is my theory... i THINK it has an issue with the "mechanical advancing business". i think everyone that's cut on it in the past year or so (the techs... are many) have tried to compensate by wrenching the bits that were wrench-able as hard as they could (therefore eliminating other slight instabilities) ultimately, they caused enough physical damage to the wrench-able bits that it nearly stopped working all-together. that is my theory. for now, the machine is usable... and the part that Barry sent improved the sections/quality/speed/my happiness by no less than 75%! (thank you Barry!!!!) to be sure... cryostats in the Mohs Practice are ABUSED. this thing has seen true combat-like conditions! i'm determined to make it make the sound... EVERY time. :) so? why would turning the dial cause the machine to start working? i would like to note here... that as a histotech that trained with someone trained by MOSES, himself... i know that wrenching on any microtome is a bad idea. if i had ever tightened a microtome as tight as someone tightened this cryostat.. that tech would've delivered a rap to my knuckles with her ruler that would make a Catholic School Nun proud! on that note... i think i'll go have a glass of wine.... and write a letter (that i'll never send) to my mother-in-law. ;) goodNIGHT! deb -- No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.1.409 / Virus Database: 268.15.18/585 - Release Date: 12/13/2006 From kurae11 <@t> yahoo.com Thu Dec 14 01:51:12 2006 From: kurae11 <@t> yahoo.com (tanapon toommanee) Date: Thu Dec 14 01:51:20 2006 Subject: [Histonet] 4 N HCL ? Message-ID: <20061214075112.94517.qmail@web50315.mail.yahoo.com> Refer to 89 ml HCl + Distilled water to make 1 liter solution .That mean 1N HCl .I would like to know 1) This is the real used in every acid 2) The meaning of 89 ml. HCl What are the ml number for the other acid that formed 1N Concentrate. 3) What are the other concentrate unit that used in general ? ____________________________________________________________________________________ Have a burning question? Go to www.Answers.yahoo.com and get answers from real people who know. From barbara.bublava <@t> meduniwien.ac.at Thu Dec 14 06:17:50 2006 From: barbara.bublava <@t> meduniwien.ac.at (Barbara Bublava) Date: Thu Dec 14 06:16:51 2006 Subject: [Histonet] paraffin problem In-Reply-To: References: Message-ID: <458140EE.4080905@meduniwien.ac.at> Du you turn off your embedding-tank between usage? I did have similar problems when I used to do that. Seems like paraffin does not like repeated melting and hardening. Since I keep paraffin melted this problem does not appear anymore. I also stirr it bevore embedding. greetings Barbara Vickroy, Jim wrote: > > > Lately we are experiencing a problem with our paraffin blocks. We use > Richard Allen Type 9 and Type 1. The tissue doesn't seem to ribbon > very well and at times the sections have some small striations, almost > like knife lines. We have checked the disposable knives and have > eliminated that variable. We use a recycled clearing agent but have > eliminated that also by using fresh chemicals. We have several tissue > processors and there is a possibility that the problem could be a > processor issue however the majority of us think that it is a paraffin > problem. We do not see any "grit" in the paraffins. We are thinking of > trying a different paraffin and wondered if anyone has switched to one > of the newer paraffins that work great. Obviously I can get as many > samples as I would like but want to see if anyone has any suggestions or > ideas that would help us eliminate some of the steps of the process. > Thanks. > > > > Jim Vickroy > > Technical Supervisor - Surgical and Autopsy Pathology > > Memorial Medical Center > > > > > > This message (including any attachments) contains confidential information intended for a > specific individual and purpose, and is protected by law. If you are not the intended recipient, > you should delete this message. Any disclosure, copying, or distribution of this message, or the > taking of any action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From gcallis <@t> montana.edu Thu Dec 14 09:33:13 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Dec 14 09:33:35 2006 Subject: [Histonet] Histoprep bx bags? In-Reply-To: <279.21f9be9d.32b221df@aol.com> References: <279.21f9be9d.32b221df@aol.com> Message-ID: <6.0.0.22.1.20061214082113.01b2a9f8@gemini.msu.montana.edu> Maybe all the merging of companies means phasing out products?? I found the little tea bag style at EMS (Electron Microscopy Sciences) Biopsy bags, Cat # 62326-05 for 500/pk We have used lens paper, folded, but you have to careful to do that carefully. Also, some lens papers shred when trying to open up and retrieve a tissue, so we use harder surface lens paper. Not ideal but works in a pinch. At 08:41 PM 12/13/2006, you wrote: >Can any tell me why the Histoprep biopsy bags have been discontinued? The >replacement bags are nylon and not what works for our lab. Has anyone >found an >exceptable replacement other than sewn nylon bags? > >Thanks, >Deb in Sacramento >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From JWEEMS <@t> sjha.org Thu Dec 14 09:36:39 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Thu Dec 14 09:37:10 2006 Subject: [Histonet] Histoprep bx bags? Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D2C90@sjhaexc02.sjha.org> Perm wrapping papers work well - you know, the hair things. Sally's has them in large boxes. Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Gayle Callis Sent: Thursday, December 14, 2006 10:33 AM To: WWmn916@aol.com; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Histoprep bx bags? Maybe all the merging of companies means phasing out products?? I found the little tea bag style at EMS (Electron Microscopy Sciences) Biopsy bags, Cat # 62326-05 for 500/pk We have used lens paper, folded, but you have to careful to do that carefully. Also, some lens papers shred when trying to open up and retrieve a tissue, so we use harder surface lens paper. Not ideal but works in a pinch. At 08:41 PM 12/13/2006, you wrote: >Can any tell me why the Histoprep biopsy bags have been discontinued? The >replacement bags are nylon and not what works for our lab. Has anyone >found an >exceptable replacement other than sewn nylon bags? > >Thanks, >Deb in Sacramento >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From PMonfils <@t> Lifespan.org Thu Dec 14 10:02:50 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Dec 14 10:03:01 2006 Subject: [Histonet] Histoprep bx bags? In-Reply-To: <6.0.0.22.1.20061214082113.01b2a9f8@gemini.msu.montana.edu> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273BD7@LSRIEXCH1.lsmaster.lifespan.org> Shandon makes nylon mesh biopsy bags in three sizes. I have used them for years. From dmcallis <@t> mcw.edu Thu Dec 14 12:08:28 2006 From: dmcallis <@t> mcw.edu (McAllister, Donna) Date: Thu Dec 14 12:08:31 2006 Subject: [Histonet] antibody organizers - database for FileMaker Pro7 Message-ID: Phil, Are you still making your FMP7 databases available? If so could you post them or email them to me? Thanks Donna -- Donna McAllister Research Technician Medical College of Wisconsin MFRC 6048 8701 Watertown Plank Rd. Milwaukee, WI 53226 414-456-8065 From TillRenee <@t> uams.edu Thu Dec 14 14:38:02 2006 From: TillRenee <@t> uams.edu (Till, Renee) Date: Thu Dec 14 14:38:28 2006 Subject: [Histonet] mammary gland embedding Message-ID: <11F927674DEBDC43B960809A7403C5D202CBF947@MAILPED.ad.uams.edu> I work with a lot of rat mammary gland tissue and depending on the age, the whole gland or most of it will usually lay flat and fit in the cassette. We recently had it suggested that we might try doing cross sections instead. Why would I do that? Is there some reason looking at a cross section view would be any different/better? The person who suggested it works with both human and animal tissue, so I thought it might be something more commonly done with human tissue where you are just taking a piece rather than the whole thing. I feel like this is one of those things I should know, but I just don't remember anything about embedding mammary tissue different ways. Thanks. Renee' Till, HT Research Assistant Arkansas Children's Nutrition Center 1212 Marshall St./N2021 Little Rock, AR 72202 Office (501)364-2785 Fax (501)364-3161 Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From emerald_lake77 <@t> yahoo.com Thu Dec 14 15:04:33 2006 From: emerald_lake77 <@t> yahoo.com (GT Hebert) Date: Thu Dec 14 15:04:44 2006 Subject: [Histonet] BACKGROUND TROUBLE - Rat IgG2b Isotype control Message-ID: <588387.20704.qm@web31702.mail.mud.yahoo.com> BACKGROUND TROUBLE USING Rat IgG2b Isotype control Hello, I am staining mouse aortic sinus sections (fixed frozen sections, 10microns) and just received a huge amount of background on my negative control sections. The antibodies I am using are Serotec?s MOMA-2 Ab and the corresponding isotype negative control, Rat IgG2b. The protocol I am using is as follows: Remove OCT using PBS (2 changes, 6 mins each) 0.3% hydrogen peroxide in water to block endogenous peroxidase (1/2 hour room temp) Either serum (according to Vector?s ABC Elite kit ? Normal Rabbit) or non serum block (DAKO) for 20 mins at rm temp. I tap off both blocks and dabbed with gauze any excess liquid. Then I add primary antibody (5ug/ml) at room temp for two hours. I wash three times in PBS + tween (0.05%) ? 3x ? 5 mins each. After wash, I continue following protocol for Vector ABC elite kit. - Add secondary Rabbit anti-rat BIOTIN (1/2 hr, room temp) - Wash as above (PBSt) - Add ABC reagent (1/2 hr room temp) - Wash again as above (PBSt) - Apply DAB chromagen (DAKO) Primary antibody work great ? lesions in aortic sinus showed nice staining (macrophages/foam cells) and there was little background throughout the entire sample. However, my negative turns COMPLETELY BROWN. I ran the negative at 5, 10 and 20 ug/ml ? there is definitely a difference between lower and higher concentrations. I stopped the chromagen after 5 minutes. This doesn?t make sense to me. Can anyone explain what might be happening and the best way I may be able to resolve this problem, making my negative become a negative again??? Thank you all in advance for your helpful advice. Sincerely, Gustave H. Scientist II Wyeth Research Cambridge MA --------------------------------- Have a burning question? Go to Yahoo! Answers and get answers from real people who know. From mhannah <@t> jhsph.edu Thu Dec 14 15:31:43 2006 From: mhannah <@t> jhsph.edu (Hannah, Michele F.) Date: Thu Dec 14 15:30:03 2006 Subject: [Histonet] STF replacement References: <5igdvl$23s13r@gateway1.jhsph.edu> Message-ID: <1D71A10BB247204A9EFFB9EED323605801916B97@XCH-VN02.sph.ad.jhsph.edu> I just called to check on an order of STF that I had placed and was told that it has been discontinued with no replacement. Just wondering if anyone else has found a replacement or knows of one. We use it exclusively for postfixation. Thanks! Michele Michele F. Hannah M.S. Department of Molecular Microbiology & Immunology Johns Hopkins Bloomberg School of Public Health From ryaskovich <@t> dir.nidcr.nih.gov Thu Dec 14 15:37:06 2006 From: ryaskovich <@t> dir.nidcr.nih.gov (Ruth Yaskovich) Date: Thu Dec 14 15:39:30 2006 Subject: [Histonet] STF replacement In-Reply-To: <1D71A10BB247204A9EFFB9EED323605801916B97@XCH-VN02.sph.ad.jhsph.edu> Message-ID: Hannah, Is that from Streck labs? We use it also. Ruth Yaskovich National Institutes of Health National Institute of Dental and Crainofacial Research Neurobiology an Pain Therapeutics Branch On 12/14/06 4:31 PM, "Hannah, Michele F." wrote: > I just called to check on an order of STF that I had placed and was told that > it has been discontinued with no replacement. Just wondering if anyone else > has found a replacement or knows of one. We use it exclusively for > postfixation. Thanks! > > > Michele > > Michele F. Hannah M.S. > Department of Molecular Microbiology & Immunology > Johns Hopkins Bloomberg School of Public Health > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Thu Dec 14 15:47:18 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Dec 14 15:47:30 2006 Subject: [Histonet] mammary gland embedding In-Reply-To: <11F927674DEBDC43B960809A7403C5D202CBF947@MAILPED.ad.uams.edu> Message-ID: <4EBFF65383B74D49995298C4976D1D5E273BD9@LSRIEXCH1.lsmaster.lifespan.org> I can't think of any reason to view such a flat, essentially homogeneous organ in cross section. The glandular structure is random in organization, not restricted to any particular plane . IMHO, the only effect of using the cross sectional view is that you will see far less of the tissue, and be far more likely to miss any localized pathology - unless you do step sections all the way through, and I see no point in that. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Till, Renee > Sent: Thursday, December 14, 2006 12:38 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] mammary gland embedding > > I work with a lot of rat mammary gland tissue and depending on the age, the > whole gland or most of it will usually lay flat and fit in the cassette. We > recently had it suggested that we might try doing cross sections instead. Why > would I do that? Is there some reason looking at a cross section view would > be any different/better? The person who suggested it works with both human > and animal tissue, so I thought it might be something more commonly done with > human tissue where you are just taking a piece rather than the whole thing. I > feel like this is one of those things I should know, but I just don't > remember anything about embedding mammary tissue different ways. Thanks. > > > > Renee' Till, HT > > Research Assistant > > Arkansas Children's Nutrition Center > > 1212 Marshall St./N2021 > > Little Rock, AR 72202 > > Office (501)364-2785 > > Fax (501)364-3161 > > > > > Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From mhannah <@t> jhsph.edu Thu Dec 14 15:54:44 2006 From: mhannah <@t> jhsph.edu (Hannah, Michele F.) Date: Thu Dec 14 15:52:07 2006 Subject: [Histonet] RE: STF References: <5igdvl$23s13r@gateway1.jhsph.edu> Message-ID: <1D71A10BB247204A9EFFB9EED323605801916B98@XCH-VN02.sph.ad.jhsph.edu> Yes, that is Streck's Tissue Fixative, should have written out the full name! Michele From kelly <@t> phenopath.com Thu Dec 14 18:34:18 2006 From: kelly <@t> phenopath.com (Kelly Turner) Date: Thu Dec 14 18:34:33 2006 Subject: [Histonet] Dako Transthyretin Antibody Message-ID: Does anyone happen to be using Dako's polyclonal rabbit anti-human prealbumin (transthyretin) antibody for IHC and willing to share their titer and pretreatment protocol? Our current vendor no longer offers this antibody and Dako's data sheet does not contain much information for its use in IHC applications. Thanks in advance! Kelly Turner, BS, HTL(ASCP), QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From tkngflght <@t> yahoo.com Fri Dec 15 00:54:45 2006 From: tkngflght <@t> yahoo.com (Cheryl) Date: Fri Dec 15 01:54:52 2006 Subject: [Histonet] Open positions--temp and permanent--Full Staff Histology In-Reply-To: Message-ID: <20061215065445.918.qmail@web50911.mail.yahoo.com> Hi everyone-- Thanks to all of you who wrote to say you liked when we rapscallion recruiters post our open positions!! I appreciate the support and try really hard not to come across as a used car salesman (and before anyone is offended, yes, I've sold used cars :)(I didn't like it--ran right back to the Histo lab!) Most of you know I'm an old-school histotech weaned on formalin--I know a lot about the positions I work to fill. I've temped at quite a few and know the labs we help really well. I don't staff labs where I wouldn't work--if I wouldn't work there, why would you? So what do we have open? We have 17+ open temp positions--all over the country. New England (7), Central and Central Plains (3), Florida (3), Texas (1 or 2) and California (2)...there are more coming open but these are ready now through January 8 (with time for Christmas). The positions range from night bench tech through grossing histotech--I pick up the phone after hours and on weekends--ask all the questions you want and we'll find one to fit (pending references). Over 40 open permanent positions. Many of these will consider temp-to-perm. There is a job to fit every tech--and we like to think we're a little different than the rest. Tell me what you want--where and what kind of lab--and we'll work FOR you. Yes, there is a list, but let's find you THE DREAM JOB, not just the next job. (Yes, they do exist.) Drop me a line, give a call--send a resume--whatever works for you. I'll be temping next week (I really do work in the labs we staff) so the sooner you call the more time I can spend with you. Also seeking: Cytotechs, Med Techs (all registries) and a Micro specialist. We're growing! Happy Holidays to all and thanks for your continued support! Cheryl (Histo for hire-LOL!) Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one Tech at a time. 281.883.7704 c 281.852.9457 o admin@fullstaff.org Sign up for the FREE monthly newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. From sonya.martin <@t> soton.ac.uk Fri Dec 15 04:09:08 2006 From: sonya.martin <@t> soton.ac.uk (Martin S.) Date: Fri Dec 15 04:09:30 2006 Subject: [Histonet] FW: Cutting frozen tumour Message-ID: <71437982F5B13A4D9A5B2669BDB89EE4023E34B9@ISS-CL-EX-V1.soton.ac.uk> ________________________________ Hi there, I'm having some trouble cutting frozen tumour samples. The tumours were stored in liquid nitrogen and i embedded them in OCT in a dish of isopentane on dry ice. After cutting, the sections seem to have a very poor structure - bits of the tissue have holes and some bits look stretched. There are also some areas that seem to have high background with all antibodies and the HRP/DAB. I dont know whether its a problem with embedding/cutting or if this is just what tumours look like! - I'm used to cutting spleen and lymph nodes. I've been using a number of different types of tumours (breast, lung, colon etc) from human and mouse. Some of the tumours I havent been able to cut at all they just crumble - I have tried as low as -30oC and as high as -12oC cutting temp. Any suggestions greatly appreciated. Sonya From matt.prideaux <@t> bbsrc.ac.uk Fri Dec 15 06:02:25 2006 From: matt.prideaux <@t> bbsrc.ac.uk (matt prideaux (RI)) Date: Fri Dec 15 06:02:17 2006 Subject: [Histonet] Methylmethacrylate to LR White? Message-ID: <84DA9D8AC9B05F4B889E7C70238CB45104C52ADB@rie2ksrv1.ri.bbsrc.ac.uk> Hi all, I need to remove the methacrylate surrounding some bone samples (I was told that a 50:50 solution of xylene and chloroform would do the trick). The problem is that I want to re-embed the samples in LR White once the methacrylate has been removed. Does anyone have any experience in this or knows if it is possible? I need to cut 10 micron thick cross-sections of mouse tibiae and these have failed to adequately infiltrate with methacrylate. With LR White being less viscous I was hoping that it would infiltrate better. Thanks Matt The information contained in this e-mail (including any attachments) is confidential and is intended for the use of the addressee only. The opinions expressed within this e-mail (including any attachments) are the opinions of the sender and do not necessarily constitute those of Roslin Institute (Edinburgh) ("the Institute") unless specifically stated by a sender who is duly authorised to do so on behalf of the Institute. From Karen.Heckford <@t> CHW.edu Fri Dec 15 07:25:13 2006 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Fri Dec 15 07:25:24 2006 Subject: [Histonet] Stainershield labels Message-ID: Hi Everyone, I was just wondering if anyone out there is using TimeMed stainer shield labels with a PowerPath program and a Zebra TLP 2844-Z label printer. I am having a little bit of problems and would like to work out the bugs. Cheers, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathology Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu From akbitting <@t> geisinger.edu Fri Dec 15 08:14:01 2006 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Dec 15 08:14:14 2006 Subject: [Histonet] Sakura AutoTec Automated Embedder Message-ID: <45826759020000C900003038@GHSGWIANW5V.GEISINGER.EDU> I'm looking for an AutoTec user to give me some feedback about their experience with this product. No vendors please. Thanks. Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From akbitting <@t> geisinger.edu Fri Dec 15 08:16:38 2006 From: akbitting <@t> geisinger.edu (Angela Bitting) Date: Fri Dec 15 08:16:55 2006 Subject: [Histonet] Richard Allan STP420 Message-ID: <458267F6020000C90000303C@GHSGWIANW5V.GEISINGER.EDU> I'm looking for a STP420 user to share their experiences with this product. No Vendors, please. Thanks, Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. From HornHV <@t> archildrens.org Fri Dec 15 09:31:24 2006 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Dec 15 09:31:47 2006 Subject: [Histonet] cardboard slide markers Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6F11@EMAIL.archildrens.org> I am looking for cardboard slide markers you use in slide filing cabinets when you remove slides to mark the space and indicate where the slides went. I ordered some once and got really nice bright yellow slide markers but I do know remember where I got them it was so long ago. The only place I have found that carries them is in Canada and they are plain white markers, which are ok. I would like to find a USA distributor. Can anyone point me in the right direction? (The Canadian company calls them memorandum markers) Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From froyer <@t> bitstream.net Fri Dec 15 09:51:20 2006 From: froyer <@t> bitstream.net (Ford Royer) Date: Fri Dec 15 09:51:31 2006 Subject: [Histonet] Leica XL (model 5010) Slide Stainer In-Reply-To: Message-ID: <004a01c72060$dd501230$7701a80a@Ford> Posting this for a college: He is asking about the Leica "XL" (model 5010) Slide Stainer. In their product information, they state that "Continuous loading and unloading of slide carrier racks ensures that the instrument is flexible even when the workload is high." His question "Do slide racks have to be manually loaded on (and off loaded) one at a time or can multiple slide racks be placed in the loading area and the instrument will automatically pick them up in sequence?" How does this work? ~ Ford Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL:? 612-839-1046 Phone:? 763-542-8725 Fax:? 763-546-4830 eMail:? clinicallab@minnesotamedical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica Hirsch Sent: Wednesday, December 13, 2006 1:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Career Opportunities in Histology Field CURRENT EMPLOYMENT OPPROTUNITIES ( HISTOLOGY, IMMUNOHISTOCHEMISTRY & PATHOLOGY ) I. Clarksburg, Maryland (Large Nationwide Clinical Labratory (division of BioReference)) 1. Histology Supervisor: Hours: Monday - Friday 12 midnight - 8:30am Pay: $25/hr + 10% night differential = $27.50/hr Benefits: Medical, Dental, 401k 2. Histology Technologist Hours: Monday - Friday 12 midnight - 8:30am Pay: $25/hr + 10% night differential = $27.50/hr Benefits: Medical, Dental, 401k 3. Grossers Hours: Monday - Friday 7pm-11:30pm Pay: $30/hr II. Elmwood Park, New Jersey ( Nationwide Clinical Laboratory) 1. Histology Technologist ( 2 positions available, immediate start ) Hours: Monday - Friday, 9:00pm - 5:30am Pay: Negotiable Benefits: Medical, Dental, 401k 2. ImmunoHistoChemistry Supervisor Hours: Monday - Friday, 7am or 8am - 4pm or 5pm Pay: Negotiable Benefits: Medical, Dental, 401k III. New York City, New York ( large world renowned Cancer Hospital ) 1. Pathologist Assistant Hours: Monday - Friday, Day shift, Full-Time Pay: $60k - $80k based on experience Benefits: Major Medical, Dental, Vision, 401k, etc. 2. Histology Technologist Hours: Monday - Friday (shift to be announced) Pay: negotiable Benefits: Medical, Dental, 401k For immediate consideration for any position: Send resumes to Jessica@medstaffservices.com OR fax to 732.238.2152 For questions call: Jessica Hirsch at 732.238.6050 ext.58 Jessica D. Hirsch Staffing Specialist Medical Staffing Services, Inc. 557 Cranbury Road East Brunswick, NJ 08816 P: (732) 238-6050 x58 F: (732) 238-2152 Jessica@medstaffservices.com http://www.medstaffservices.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Fri Dec 15 10:18:23 2006 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Fri Dec 15 10:18:47 2006 Subject: [Histonet] Methylmethacrylate to LR White? In-Reply-To: <84DA9D8AC9B05F4B889E7C70238CB45104C52ADB@rie2ksrv1.ri.bbsrc.ac.uk> Message-ID: <200612151618.kBFGISCS014808@pro12.abac.com> Matt, Methyl methacrylate can be removed with xylene, but glycol methacrylate can not ever be completely removed, I believe LR White is a glycol methacrylate. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of matt prideaux (RI) Sent: Friday, December 15, 2006 5:02 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Methylmethacrylate to LR White? Hi all, I need to remove the methacrylate surrounding some bone samples (I was told that a 50:50 solution of xylene and chloroform would do the trick). The problem is that I want to re-embed the samples in LR White once the methacrylate has been removed. Does anyone have any experience in this or knows if it is possible? I need to cut 10 micron thick cross-sections of mouse tibiae and these have failed to adequately infiltrate with methacrylate. With LR White being less viscous I was hoping that it would infiltrate better. Thanks Matt The information contained in this e-mail (including any attachments) is confidential and is intended for the use of the addressee only. The opinions expressed within this e-mail (including any attachments) are the opinions of the sender and do not necessarily constitute those of Roslin Institute (Edinburgh) ("the Institute") unless specifically stated by a sender who is duly authorised to do so on behalf of the Institute. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Fri Dec 15 10:20:33 2006 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Fri Dec 15 10:20:52 2006 Subject: [Histonet] FW: Cutting frozen tumour In-Reply-To: <71437982F5B13A4D9A5B2669BDB89EE4023E34B9@ISS-CL-EX-V1.soton.ac.uk> Message-ID: <200612151620.kBFGKbHS015948@pro12.abac.com> Sonya, If your tumors are stored in LN without any cryoprotectant (OCT or Sucrose) you will probably have trouble cutting them. I would suggest that the tumors be cryoprotected before freezing and storing in LN. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Martin S. Sent: Friday, December 15, 2006 3:09 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Cutting frozen tumour ________________________________ Hi there, I'm having some trouble cutting frozen tumour samples. The tumours were stored in liquid nitrogen and i embedded them in OCT in a dish of isopentane on dry ice. After cutting, the sections seem to have a very poor structure - bits of the tissue have holes and some bits look stretched. There are also some areas that seem to have high background with all antibodies and the HRP/DAB. I dont know whether its a problem with embedding/cutting or if this is just what tumours look like! - I'm used to cutting spleen and lymph nodes. I've been using a number of different types of tumours (breast, lung, colon etc) from human and mouse. Some of the tumours I havent been able to cut at all they just crumble - I have tried as low as -30oC and as high as -12oC cutting temp. Any suggestions greatly appreciated. Sonya _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From atebo <@t> aahs.org Fri Dec 15 10:22:56 2006 From: atebo <@t> aahs.org (Tebo, Andrea) Date: Fri Dec 15 10:23:39 2006 Subject: [Histonet] Leica XL (model 5010) Slide Stainer Message-ID: <00790F10D0600A41AEE8899901E533A61044B397@aamcexch.aamc.org> The Leica Autostainer XL that we have here is one rack at a time loading and unloading. We have to wait for the one rack to be picked up before we can place another rack into the loading station. And the same for the unloading station. Sincerely, Andrea Tebo, HT(ASCP) Histopathology Supervisor Anne Arundel Medical Center Annapolis, Maryland -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ford Royer Sent: Friday, December 15, 2006 10:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica XL (model 5010) Slide Stainer Posting this for a college: He is asking about the Leica "XL" (model 5010) Slide Stainer. In their product information, they state that "Continuous loading and unloading of slide carrier racks ensures that the instrument is flexible even when the workload is high." His question "Do slide racks have to be manually loaded on (and off loaded) one at a time or can multiple slide racks be placed in the loading area and the instrument will automatically pick them up in sequence?" How does this work? ~ Ford Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL:? 612-839-1046 Phone:? 763-542-8725 Fax:? 763-546-4830 eMail:? clinicallab@minnesotamedical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica Hirsch Sent: Wednesday, December 13, 2006 1:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Career Opportunities in Histology Field CURRENT EMPLOYMENT OPPROTUNITIES ( HISTOLOGY, IMMUNOHISTOCHEMISTRY & PATHOLOGY ) I. Clarksburg, Maryland (Large Nationwide Clinical Labratory (division of BioReference)) 1. Histology Supervisor: Hours: Monday - Friday 12 midnight - 8:30am Pay: $25/hr + 10% night differential = $27.50/hr Benefits: Medical, Dental, 401k 2. Histology Technologist Hours: Monday - Friday 12 midnight - 8:30am Pay: $25/hr + 10% night differential = $27.50/hr Benefits: Medical, Dental, 401k 3. Grossers Hours: Monday - Friday 7pm-11:30pm Pay: $30/hr II. Elmwood Park, New Jersey ( Nationwide Clinical Laboratory) 1. Histology Technologist ( 2 positions available, immediate start ) Hours: Monday - Friday, 9:00pm - 5:30am Pay: Negotiable Benefits: Medical, Dental, 401k 2. ImmunoHistoChemistry Supervisor Hours: Monday - Friday, 7am or 8am - 4pm or 5pm Pay: Negotiable Benefits: Medical, Dental, 401k III. New York City, New York ( large world renowned Cancer Hospital ) 1. Pathologist Assistant Hours: Monday - Friday, Day shift, Full-Time Pay: $60k - $80k based on experience Benefits: Major Medical, Dental, Vision, 401k, etc. 2. Histology Technologist Hours: Monday - Friday (shift to be announced) Pay: negotiable Benefits: Medical, Dental, 401k For immediate consideration for any position: Send resumes to Jessica@medstaffservices.com OR fax to 732.238.2152 For questions call: Jessica Hirsch at 732.238.6050 ext.58 Jessica D. Hirsch Staffing Specialist Medical Staffing Services, Inc. 557 Cranbury Road East Brunswick, NJ 08816 P: (732) 238-6050 x58 F: (732) 238-2152 Jessica@medstaffservices.com http://www.medstaffservices.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ---------------------------------------------------------------------- This message [including any attachments] contains information intended for a specific individual[s] and purpose that may be confidential or otherwise legally protected from disclosure. Any review, use, distribution, disclosure of contents, or copying of the message is strictly prohibited. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately without copying or disclosing the information. From pruegg <@t> ihctech.net Fri Dec 15 10:28:32 2006 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Fri Dec 15 10:28:57 2006 Subject: [Histonet] mammary gland embedding In-Reply-To: <4EBFF65383B74D49995298C4976D1D5E273BD9@LSRIEXCH1.lsmaster.lifespan.org> Message-ID: <200612151628.kBFGSaNJ020410@pro12.abac.com> I agree with Paul on this, I cannot see any reason to embed mammary tissue, animal or human as cross-section. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Thursday, December 14, 2006 2:47 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] mammary gland embedding I can't think of any reason to view such a flat, essentially homogeneous organ in cross section. The glandular structure is random in organization, not restricted to any particular plane . IMHO, the only effect of using the cross sectional view is that you will see far less of the tissue, and be far more likely to miss any localized pathology - unless you do step sections all the way through, and I see no point in that. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Till, Renee > Sent: Thursday, December 14, 2006 12:38 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] mammary gland embedding > > I work with a lot of rat mammary gland tissue and depending on the age, the > whole gland or most of it will usually lay flat and fit in the cassette. We > recently had it suggested that we might try doing cross sections instead. Why > would I do that? Is there some reason looking at a cross section view would > be any different/better? The person who suggested it works with both human > and animal tissue, so I thought it might be something more commonly done with > human tissue where you are just taking a piece rather than the whole thing. I > feel like this is one of those things I should know, but I just don't > remember anything about embedding mammary tissue different ways. Thanks. > > > > Renee' Till, HT > > Research Assistant > > Arkansas Children's Nutrition Center > > 1212 Marshall St./N2021 > > Little Rock, AR 72202 > > Office (501)364-2785 > > Fax (501)364-3161 > > > > > Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Fri Dec 15 10:58:39 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Dec 15 10:58:55 2006 Subject: These are different RE: [Histonet] Methylmethacrylate to LR White? In-Reply-To: <200612151618.kBFGISCS014808@pro12.abac.com> References: <84DA9D8AC9B05F4B889E7C70238CB45104C52ADB@rie2ksrv1.ri.bbsrc.ac.uk> <200612151618.kBFGISCS014808@pro12.abac.com> Message-ID: <6.0.0.22.1.20061215093316.01b3e400@gemini.msu.montana.edu> LR White is not the same glycol methacrylate although it does contain some type of methacrylate resin. It's chemical components are 80% polyhydroxy substituted bisphenol, a dimethacrylate resin 19.6% C12 methacrylate ester Catalyst is 0.9% benzoyl peroxide One difference is LR white, although low viscosity, can be used for electron microscopy while glycol methacrylate is not advisable for EM, it "gums" up the 'scope. I was never successful at removing polymerized methyl methacrylate (MMA) from internal portions of a bone. However, I did remove it from external bone surfaces after a rapid polymerization took place. Bubbled MMA was chipped and ground away first, then the remaining block was immersed into pure methacrylate monomer. The methacrylate monomer acts as MMA's own solvent. After the bubbly mess was gone from the outside, but not the inside of the bone which was reembedded into MMA. MMA can be removed from thin sections (3 to 5 um, maybe thicker) with other solvents, even the monomer and 60C xylene was suggested by Neil Hand. Chloroform may not be advisable due to its carcingenic nature. Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 At 09:18 AM 12/15/2006, you wrote: >Matt, >Methyl methacrylate can be removed with xylene, but glycol methacrylate can >not ever be completely removed, I believe LR White is a glycol methacrylate. >Patsy > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of matt >prideaux (RI) >Sent: Friday, December 15, 2006 5:02 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Methylmethacrylate to LR White? > >Hi all, > >I need to remove the methacrylate surrounding some bone samples (I was >told that a 50:50 solution of xylene and chloroform would do the trick). >The problem is that I want to re-embed the samples in LR White once the >methacrylate has been removed. Does anyone have any experience in this >or knows if it is possible? I need to cut 10 micron thick cross-sections >of mouse tibiae and these have failed to adequately infiltrate with >methacrylate. With LR White being less viscous I was hoping that it >would infiltrate better. > >Thanks > >Matt > > The information contained in this e-mail (including any attachments) is >confidential and is intended for the use of the addressee only. The >opinions expressed within this e-mail (including any attachments) are >the opinions of the sender and do not necessarily constitute those of >Roslin Institute (Edinburgh) ("the Institute") unless specifically >stated by a sender who is duly authorised to do so on behalf of the >Institute. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AGrobe2555 <@t> aol.com Fri Dec 15 11:25:05 2006 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Fri Dec 15 11:25:21 2006 Subject: [Histonet] Cardboard slide markers Message-ID: Hazel, Could you cut some out of heavy fluorescent cardstock from your local office supply store? Might be cheaper and easier...... Albert From cforster <@t> umn.edu Fri Dec 15 11:33:39 2006 From: cforster <@t> umn.edu (Colleen Forster) Date: Fri Dec 15 11:31:46 2006 Subject: [Histonet] Cardboard slide markers In-Reply-To: References: Message-ID: <4582DC73.9030308@umn.edu> They are called Memo markers and Fisher has them. Cat# 072-12-112. These are NOT that expensive and NO, having to sit and cut them ISN'T easier...it is a pain! Colleen Forster U of MN AGrobe2555@aol.com wrote: > Hazel, > Could you cut some out of heavy fluorescent cardstock from your local office > supply store? Might be cheaper and easier...... > Albert > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > . > > From settembr <@t> umdnj.edu Fri Dec 15 12:03:53 2006 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Fri Dec 15 12:04:31 2006 Subject: [Histonet] Dako Transthyretin Antibody Message-ID: Hello Kelly, I use Dako's Prealbumin on FFPE human tissue with a choriod plexus as a positive control. I use no pretreatment and I use it at 1:8000 with nice results. Good Luck. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Kelly Turner 12/14/06 7:34 PM >>> Does anyone happen to be using Dako's polyclonal rabbit anti-human prealbumin (transthyretin) antibody for IHC and willing to share their titer and pretreatment protocol? Our current vendor no longer offers this antibody and Dako's data sheet does not contain much information for its use in IHC applications. Thanks in advance! Kelly Turner, BS, HTL(ASCP), QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gu.lang <@t> gmx.at Fri Dec 15 12:19:55 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Fri Dec 15 12:18:27 2006 Subject: AW: [Histonet] Leica XL (model 5010) Slide Stainer In-Reply-To: <004a01c72060$dd501230$7701a80a@Ford> Message-ID: <000001c72075$9fbcee10$c812a8c0@dielangs.at> You can install one to three loading jars, and one to three unloading jars instead of the usual staining jars. So as you like you can put maximal three racks at one time in the machine. You learn the instrument to recognise the whished stain through coloured chips. When the racks are in the stainer, it makes a "plan" and takes the racks following this. Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Ford Royer Gesendet: Freitag, 15. Dezember 2006 16:51 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Leica XL (model 5010) Slide Stainer Posting this for a college: He is asking about the Leica "XL" (model 5010) Slide Stainer. In their product information, they state that "Continuous loading and unloading of slide carrier racks ensures that the instrument is flexible even when the workload is high." His question "Do slide racks have to be manually loaded on (and off loaded) one at a time or can multiple slide racks be placed in the loading area and the instrument will automatically pick them up in sequence?" How does this work? ~ Ford Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL:? 612-839-1046 Phone:? 763-542-8725 Fax:? 763-546-4830 eMail:? clinicallab@minnesotamedical.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jessica Hirsch Sent: Wednesday, December 13, 2006 1:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Career Opportunities in Histology Field CURRENT EMPLOYMENT OPPROTUNITIES ( HISTOLOGY, IMMUNOHISTOCHEMISTRY & PATHOLOGY ) I. Clarksburg, Maryland (Large Nationwide Clinical Labratory (division of BioReference)) 1. Histology Supervisor: Hours: Monday - Friday 12 midnight - 8:30am Pay: $25/hr + 10% night differential = $27.50/hr Benefits: Medical, Dental, 401k 2. Histology Technologist Hours: Monday - Friday 12 midnight - 8:30am Pay: $25/hr + 10% night differential = $27.50/hr Benefits: Medical, Dental, 401k 3. Grossers Hours: Monday - Friday 7pm-11:30pm Pay: $30/hr II. Elmwood Park, New Jersey ( Nationwide Clinical Laboratory) 1. Histology Technologist ( 2 positions available, immediate start ) Hours: Monday - Friday, 9:00pm - 5:30am Pay: Negotiable Benefits: Medical, Dental, 401k 2. ImmunoHistoChemistry Supervisor Hours: Monday - Friday, 7am or 8am - 4pm or 5pm Pay: Negotiable Benefits: Medical, Dental, 401k III. New York City, New York ( large world renowned Cancer Hospital ) 1. Pathologist Assistant Hours: Monday - Friday, Day shift, Full-Time Pay: $60k - $80k based on experience Benefits: Major Medical, Dental, Vision, 401k, etc. 2. Histology Technologist Hours: Monday - Friday (shift to be announced) Pay: negotiable Benefits: Medical, Dental, 401k For immediate consideration for any position: Send resumes to Jessica@medstaffservices.com OR fax to 732.238.2152 For questions call: Jessica Hirsch at 732.238.6050 ext.58 Jessica D. Hirsch Staffing Specialist Medical Staffing Services, Inc. 557 Cranbury Road East Brunswick, NJ 08816 P: (732) 238-6050 x58 F: (732) 238-2152 Jessica@medstaffservices.com http://www.medstaffservices.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Fri Dec 15 12:23:30 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Dec 15 12:23:43 2006 Subject: [Histonet] Re: cardboard slide markers Message-ID: <389.1183e3ae.32b44222@aol.com> Hazel Horn is >>looking for cardboard slide markers you use in slide filing cabinets when you remove slides to mark the space and indicate where the slides went.<< You can make extremely durable slide filing cabinet markers by cutting 3.5 x 1 inch strips (use a paper cutter) out of exposed developed (the blacker the better) X-ray film. The specimen radiograms your pathologists receive (or should receive!) with wire-localization breast biopsy specimens are a convenient source of film - but hurry, radiology departments are going digital in a hurry, and film will soon go the way of wet-tank developing (hey, I AM a geezer to remember that!). Bob Richmond Samurai Pathologist Knoxville TN From jnocito <@t> satx.rr.com Fri Dec 15 12:26:42 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Dec 15 12:26:52 2006 Subject: [Histonet] cardboard slide markers References: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6F11@EMAIL.archildrens.org> Message-ID: <000701c72076$925a2700$be604542@yourxhtr8hvc4p> Hazel, Try Lab Storage Systems. They also have some for blocks. They are a little shorter than the ones for slides. Both types comes in a variety of colors. Joe Nocito ----- Original Message ----- From: "Horn, Hazel V" To: "Histo Net list server" Sent: Friday, December 15, 2006 9:31 AM Subject: [Histonet] cardboard slide markers I am looking for cardboard slide markers you use in slide filing cabinets when you remove slides to mark the space and indicate where the slides went. I ordered some once and got really nice bright yellow slide markers but I do know remember where I got them it was so long ago. The only place I have found that carries them is in Canada and they are plain white markers, which are ok. I would like to find a USA distributor. Can anyone point me in the right direction? (The Canadian company calls them memorandum markers) Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cgfields <@t> lexhealth.org Fri Dec 15 12:37:57 2006 From: cgfields <@t> lexhealth.org (Carole Fields) Date: Fri Dec 15 12:38:20 2006 Subject: [Histonet] Re: cardboard slide markers Message-ID: I made my own in the computer and print them on card stock or semi-card stock. Then when the techs get bored or get an extra minute they cut them up with a paper cutter. They have all the pertinent info on the tabs....just let me know. I will be glad to send it to anyone needing them. I have one for blocks also. Carole Fields, HT,ASCP Pathology Supervisor Lexington Medical Center 2720 Sunset Blvd. W. Columbia, SC 29169 -----Original Message----- From: RSRICHMOND@aol.com [mailto:RSRICHMOND@aol.com] Sent: Friday, December 15, 2006 1:24 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: cardboard slide markers Hazel Horn is >>looking for cardboard slide markers you use in slide filing cabinets when you remove slides to mark the space and indicate where the slides went.<< You can make extremely durable slide filing cabinet markers by cutting 3.5 x 1 inch strips (use a paper cutter) out of exposed developed (the blacker the better) X-ray film. The specimen radiograms your pathologists receive (or should receive!) with wire-localization breast biopsy specimens are a convenient source of film - but hurry, radiology departments are going digital in a hurry, and film will soon go the way of wet-tank developing (hey, I AM a geezer to remember that!). Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________ This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachments, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed above if one is provided. From CIngles <@t> uwhealth.org Fri Dec 15 13:14:07 2006 From: CIngles <@t> uwhealth.org (Ingles Claire) Date: Fri Dec 15 13:14:16 2006 Subject: [Histonet] GLUT-1 marker Message-ID: <08A0A863637F1349BBFD83A96B27A50A11FFC9@uwhis-xchng3.uwhis.hosp.wisc.edu> Help! I have a doc here looking for someone who does the GLUT-1 (IHC) stain. I have looked all over and can't find any reference labs (or anyone else for that matter) who does this test. Plenty of papers, etc., but no one actually doing the test. Anyone out there do this test? I think the Doc is just trying to drive us all crazy but I may be a bit paranoid by now. TGIF! Any help is greatly appreciated. Claire Ingles UW Hospital and Clinics Madison WI From mauger <@t> email.chop.edu Fri Dec 15 13:22:18 2006 From: mauger <@t> email.chop.edu (Joanne Mauger) Date: Fri Dec 15 13:23:12 2006 Subject: [Histonet] GLUT-1 marker Message-ID: Hi All, We do Glut-1, but we are not a reference lab. You can buy the antibody from Labvision (Neomarkers) #RB-9052. It's a rabbit polyclonal-we use it at 1:200 with citrate buffer pH6 antigen retrieval. Works weel- Jo Mauger From pmarcum <@t> vet.upenn.edu Fri Dec 15 13:48:21 2006 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Fri Dec 15 13:48:37 2006 Subject: [Histonet] Methylmethacrylate to LR White? In-Reply-To: <200612151618.kBFGISCS014808@pro12.abac.com> References: <84DA9D8AC9B05F4B889E7C70238CB45104C52ADB@rie2ksrv1.ri.bbsrc.ac.uk> <200612151618.kBFGISCS014808@pro12.abac.com> Message-ID: <6.2.5.6.2.20061215144737.01c405e0@vet.upenn.edu> Patsy is correct GMA can not be removed and it is part of the LR White formula. Pam Marcum At 11:18 AM 12/15/2006, pruegg@ihctech.net wrote: >Matt, >Methyl methacrylate can be removed with xylene, but glycol methacrylate can >not ever be completely removed, I believe LR White is a glycol methacrylate. >Patsy > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of matt >prideaux (RI) >Sent: Friday, December 15, 2006 5:02 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Methylmethacrylate to LR White? > >Hi all, > >I need to remove the methacrylate surrounding some bone samples (I was >told that a 50:50 solution of xylene and chloroform would do the trick). >The problem is that I want to re-embed the samples in LR White once the >methacrylate has been removed. Does anyone have any experience in this >or knows if it is possible? I need to cut 10 micron thick cross-sections >of mouse tibiae and these have failed to adequately infiltrate with >methacrylate. With LR White being less viscous I was hoping that it >would infiltrate better. > >Thanks > >Matt > > The information contained in this e-mail (including any attachments) is >confidential and is intended for the use of the addressee only. The >opinions expressed within this e-mail (including any attachments) are >the opinions of the sender and do not necessarily constitute those of >Roslin Institute (Edinburgh) ("the Institute") unless specifically >stated by a sender who is duly authorised to do so on behalf of the >Institute. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From HornHV <@t> archildrens.org Fri Dec 15 14:40:53 2006 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Fri Dec 15 14:41:09 2006 Subject: [Histonet] GLUT-1 marker In-Reply-To: <08A0A863637F1349BBFD83A96B27A50A11FFC9@uwhis-xchng3.uwhis.hosp.wisc.edu> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6F17@EMAIL.archildrens.org> We do the Glut stain in our lab. We get the antibody from Dako I believe. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ingles Claire Sent: Friday, December 15, 2006 1:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] GLUT-1 marker Help! I have a doc here looking for someone who does the GLUT-1 (IHC) stain. I have looked all over and can't find any reference labs (or anyone else for that matter) who does this test. Plenty of papers, etc., but no one actually doing the test. Anyone out there do this test? I think the Doc is just trying to drive us all crazy but I may be a bit paranoid by now. TGIF! Any help is greatly appreciated. Claire Ingles UW Hospital and Clinics Madison WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From ancillarypath <@t> mac.com Sat Dec 16 12:37:57 2006 From: ancillarypath <@t> mac.com (ancillarypath@mac.com) Date: Sat Dec 16 12:38:18 2006 Subject: [Histonet] Re: GLUT-1 antibody In-Reply-To: <200612161809.kBGI9IlR025355@mac.com> References: <200612161809.kBGI9IlR025355@mac.com> Message-ID: We run GLUT-1 in our reference lab. Please contact us directly if you would like to send a sample. H. Yaziji, M.D. ================================ Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories 7000 62nd Avenue, PH-C Miami, FL 33143 T 305-740-4440 F. 786-513-0175 www.vitromolecular.com From bakevictoria <@t> gmail.com Sat Dec 16 20:11:04 2006 From: bakevictoria <@t> gmail.com (Victoria Baker) Date: Sat Dec 16 20:11:11 2006 Subject: [Histonet] Off topic- hit delete if not a cat/animal lover - with my apologies Message-ID: <4f016b690612161811x2c78c73p449c770865de56f7@mail.gmail.com> Hi For anyone who feels that is not proper use of the histology list server, I apologize but I'm desperate to find homes for about a dozen cats that range in age from 8 weeks to approximately 2 years. I've been foster caring for stray cats in my home that is located in Rockland County NYS. For career purposes I'm moving to Maryland and cannot take all of the cats with me (although I wish I could). I need to find homes or foster care for these cats ASAP and if anyone knows of anyone who would like a cat/kitten please let me know. All are "domestic tiger type cats" some short hair and a couple long hair. I have 3 that are what I call mosaic marble cats they have black/grey and tan markings all over. Sweet tempered animals that just want a home with a loving caring family. If any of you know of anyone or know of an agency that can help me I would be most grateful. Thank you. Vikki Baker From brunella.spaggiari <@t> nemo.unipr.it Mon Dec 18 02:25:52 2006 From: brunella.spaggiari <@t> nemo.unipr.it (Brunella Spaggiari) Date: Mon Dec 18 02:27:42 2006 Subject: [Histonet] Von Kossa stain Message-ID: <002701c7227e$21949dc0$ba3a4ea0@PC186Gabbi> Hello everybody, we work on undelcalcified bone tissue (human, rabbit) fixed in PFA 4% and embedded in methylmethacrilate (MMA). >From our samples we obtain 50 ?m thick sections which can contain titanium implants. We tried to perform Von Kossa stain, always on undeplasticized sections, but we met several problems: - calcium deposits after silver nitrate+UV light did not appear black but brown, and, after sodium thiosulphate, brown stain turns more and more light; - we could not find a suitable counterstain for osteoid: nuclear fast red and neutral red do not stain at all, basic or acid fuchsin stain too much and cover the underlying brown.. Some suggestions? Are there other good histological stainings for undeplasticized bone sections in your experience? Thank you! Brunella From GAshton <@t> PICR.man.ac.uk Mon Dec 18 08:45:19 2006 From: GAshton <@t> PICR.man.ac.uk (Garry Ashton) Date: Mon Dec 18 08:45:29 2006 Subject: [Histonet] cryostat Message-ID: Dear all, We have an old cryostat that is surplus to requirements (Richert Jung Cryocut E) It has not been plugged in for a couple of years, but when I tried it yesterday it seemed to get down to temperature. However I can't guarantee it works OK. There are also the transport costs to consider. However if anybody in the UK would like it, then let me know. Many thanks Garry Garry Ashton PICR Manchester UK -------------------------------------------------------- This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the University of Manchester. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From gcallis <@t> montana.edu Mon Dec 18 09:37:31 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Dec 18 09:37:39 2006 Subject: [Histonet] Von Kossa stain In-Reply-To: <002701c7227e$21949dc0$ba3a4ea0@PC186Gabbi> References: <002701c7227e$21949dc0$ba3a4ea0@PC186Gabbi> Message-ID: <6.0.0.22.1.20061218083425.01b5a788@gemini.msu.montana.edu> Toluidine Blue, Sterchi method, or MacNeals Tetracrhome (in house recipe - we found the commercial solution did not work well) - I will be happy to foward methods if you wish. When you do basic fuchsin, you need to cut back on concentration of the basic fuchsin and also the time of staining. Take on section and play with the timing/dye concentration. At 01:25 AM 12/18/2006, you wrote: >Hello everybody, > >we work on undelcalcified bone tissue (human, rabbit) fixed in PFA 4% and >embedded in methylmethacrilate (MMA). > >From our samples we obtain 50 ?m thick sections which can contain > titanium implants. >We tried to perform Von Kossa stain, always on undeplasticized sections, >but we met several problems: > >- calcium deposits after silver nitrate+UV light did not appear black but >brown, and, after sodium thiosulphate, brown stain turns more and more light; > >- we could not find a suitable counterstain for osteoid: nuclear fast red >and neutral red do not stain at all, basic or acid fuchsin stain too much >and cover the underlying brown.. > >Some suggestions? Are there other good histological stainings for >undeplasticized bone sections in your experience? > >Thank you! >Brunella >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From debbiekeith <@t> cox.net Mon Dec 18 11:37:42 2006 From: debbiekeith <@t> cox.net (Debbie Keith) Date: Mon Dec 18 11:37:50 2006 Subject: [Histonet] i got approval to buy a new cryostat ... Message-ID: <5.2.0.9.0.20061218103211.00c3fe00@pop.central.cox.net> hey all! on friday, i got the official ok to find a new Leica 1850. i spoke to Jan Minshew with Leica (she's an HTL!) and she helped me understand the residual issues i was having with the cryostat. it is definitely a solid backup cryostat now. it would be a FINE main unit... but if i'm going to get a NEW one... i may as well get the 1850, no? ;) Thank you , Jan!!! you were helpful and SO right! thanks to everyone here for all your help. :) any ideas where i can get the best price on a new 1850? i gotta get it in the next two weeks... (year-end-taxes-stuff) deb -- No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.1.409 / Virus Database: 268.15.24/592 - Release Date: 12/18/2006 From debbiekeith <@t> cox.net Mon Dec 18 11:46:34 2006 From: debbiekeith <@t> cox.net (Debbie Keith) Date: Mon Dec 18 11:46:43 2006 Subject: [Histonet] microwave processing on a budget... Message-ID: <5.2.0.9.0.20061218103814.03077910@pop.central.cox.net> Hey all... i have a few questions about microwave processing skin biopsies utilizing a microprocessor. i've looked into protocols and equipment but figured this would be the best/quickest sources of trial/error stories. i want to get a used lab microwave but want to hear good/bad stories from the folks in the trenches. :) marketers can sell me ANYTHING!! i might be the ONLY person that bought the magic bullet food processor AND the ginsu knife. (for the record, they are NOT as sharp after cutting through a tin can. fyi.) i purchased a used shandon hypercenter from a surplus lab equipment place... and it's been such a NIGHTMARE. with enclosed processors there are so many possible issues. gaskets/circuit boards/cable ports.... i'm starting to think micro-processing would keep the biomedical-nightmares to a minimum. have i inhaled too many fumes or could i be onto something? i REALLY don't want to resort to an old carousel processor. the specimens will be limited to small shave/punch skin biopsies and the runs will never be more than 20 blocks. i would love to hear from anyone that has done this with good results. i'd like to hear the "avoid THIS at all costs...." stories as well. :) histonette is such a wonderful resource! you guys are AWESOME! debbie -- No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.1.409 / Virus Database: 268.15.24/592 - Release Date: 12/18/2006 From gcallis <@t> montana.edu Mon Dec 18 12:10:41 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Dec 18 12:10:57 2006 Subject: [Histonet] i got approval to buy a new cryostat ... In-Reply-To: <5.2.0.9.0.20061218103211.00c3fe00@pop.central.cox.net> References: <5.2.0.9.0.20061218103211.00c3fe00@pop.central.cox.net> Message-ID: <6.0.0.22.1.20061218110817.01b23398@gemini.msu.montana.edu> You need to contact your local Leica sales rep and ask for a quote. You will love the 1850, we have three humming along all the time. At 10:37 AM 12/18/2006, you wrote: >hey all! > >on friday, i got the official ok to find a new Leica 1850. > >i spoke to Jan Minshew with Leica (she's an HTL!) and she helped me >understand the residual issues i was having with the cryostat. it is >definitely a solid backup cryostat now. it would be a FINE main unit... >but if i'm going to get a NEW one... i may as well get the 1850, no? ;) > >Thank you , Jan!!! you were helpful and SO right! > >thanks to everyone here for all your help. :) > >any ideas where i can get the best price on a new 1850? i gotta get it in >the next two weeks... (year-end-taxes-stuff) > >deb > > >-- >No virus found in this outgoing message. >Checked by AVG Free Edition. >Version: 7.1.409 / Virus Database: 268.15.24/592 - Release Date: 12/18/2006 > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From nancy.troiano <@t> yale.edu Mon Dec 18 12:38:38 2006 From: nancy.troiano <@t> yale.edu (Nancy W. Troiano) Date: Mon Dec 18 12:38:50 2006 Subject: [Histonet] Von Kossa Stain Message-ID: <5.2.1.1.2.20061218133655.02784d90@email.med.yale.edu> We use methyl green pyronin as a counterstain for our Von Kossa stain on 4 -5 micron deplasticized sections of undecalcified bone embedded in MMA. I don't know if that stain will work on thick undeplasticized sections, though. Another possible counterstain that we use occasionally is toluidine blue, pH 3.7 which shows up the cells nicely. From tkngflght <@t> yahoo.com Mon Dec 18 15:02:27 2006 From: tkngflght <@t> yahoo.com (Cheryl) Date: Mon Dec 18 15:02:33 2006 Subject: [Histonet] 25 open temp positions and even more permanent jobs... Message-ID: <260304.81593.qm@web50902.mail.yahoo.com> Hi All- We've been blessed with an exclusive contract for 13 week temporary positions all over the country. If you've EVER thought of temping, now is your chance! We'll be moving techs into place across the next three weeks (you can stay home for Christmas and still get signed up now to plan ahead!) Temporary Locations include (these are just some--there are more): Western Florida Connecticut North Carolina Southern California Oklahoma New York Kansas Western Ohio Permanent locations include those listed above and: South Carolina Texas Maine New Hampshire Wisconsin Colorado And a few more coming in daily. We're accepting referrals for all of these so call if you want more details. Tiffany is in the office 281.852.9457 or 800.756.3309 and I'm temping and available by cell 281.883.7704 and toll-free 800.756.3309 Give us a call, email and if you can--send a current resume: admin@fullstaff.org We look forward to hearing from you!! Cheryl Kerry, HT(ASCP) Full Staff Inc Staffing the AP Lab, one great tech at a time. 800.756.3309 phone and fax 281.852.9457 office 281.883.7704 cell admin@fullstaff.org From Tony_Reilly <@t> health.qld.gov.au Tue Dec 19 00:51:38 2006 From: Tony_Reilly <@t> health.qld.gov.au (Anthony Reilly) Date: Tue Dec 19 01:02:46 2006 Subject: [Histonet] microwave processing on a budget... Message-ID: Hi Debbie I will be interested to hear other subscriber's comments with regard to microwave processing of skin. In our lab we do not have a microwave processor but we do microwave fix on a regular basis. Having said that we always resist microwave fixing skin as the process damages the skin tissue giving it that "overcooked" appearance which occurs when tissue has been exposed to too much heat during processing. This is a genuine artifact as we have found in a whole container of breast tissue which has been microwave fixed only the nipple will be damaged. With regard to your second hand processor, it is a bit like buying a second hand lawn mower, you get what you paid for. If it is a shandon from circa late 1980s early 1990s they were notoriously flawed, particularly the heating bands for the retort.( Current models are fine) A new modern standard processor will give you good morphology on small skins on a 2-4 hour processing run and larger skins dependent on the amount of fat present will process well on 9-12 hour cycles. While microwave processors have their place in Histology for the high throughput laboratories that are running 18-24 hours per day, if your lab is working predominately day shifts only, I do not believe the advantages of the microwave processors will be achieved and therefore the extra cost of purchase is not warranted. As well as this most microwave processors require the user to purchase the manufacturer's "secret" proprietry solution/s creating further ongoing costs. regards Tony Reilly Chief Scientist Anatomical Pathology QHPS-Prince Charles Hospital Rode Rd Chermside Q 4032 Australia Ph: 07 3139 4543 Fax: 07 3193 4546 tony_reilly@health.qld.gov.au >>> Debbie Keith 12/19/06 3:46 am >>> Hey all... i have a few questions about microwave processing skin biopsies utilizing a microprocessor. i've looked into protocols and equipment but figured this would be the best/quickest sources of trial/error stories. i want to get a used lab microwave but want to hear good/bad stories from the folks in the trenches. :) marketers can sell me ANYTHING!! i might be the ONLY person that bought the magic bullet food processor AND the ginsu knife. (for the record, they are NOT as sharp after cutting through a tin can. fyi.) i purchased a used shandon hypercenter from a surplus lab equipment place... and it's been such a NIGHTMARE. with enclosed processors there are so many possible issues. gaskets/circuit boards/cable ports.... i'm starting to think micro-processing would keep the biomedical-nightmares to a minimum. have i inhaled too many fumes or could i be onto something? i REALLY don't want to resort to an old carousel processor. the specimens will be limited to small shave/punch skin biopsies and the runs will never be more than 20 blocks. i would love to hear from anyone that has done this with good results. i'd like to hear the "avoid THIS at all costs...." stories as well. :) histonette is such a wonderful resource! you guys are AWESOME! debbie -- No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.1.409 / Virus Database: 268.15.24/592 - Release Date: 12/18/2006 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***************************************************************** This email, including any attachments sent with it, is confidential and for the sole use of the intended recipient(s). This confidentiality is not waived or lost, if you receive it and you are not the intended recipient(s), or if it is transmitted/ received in error. Any unauthorised use, alteration, disclosure, distribution or review of this email is strictly prohibited. The information contained in this email, including any attachment sent with it, may be subject to a statutory duty of confidentiality if it relates to health service matters. If you are not the intended recipient(s), or if you have received this email in error, you are asked to immediately notify the sender by telephone collect on Australia +61 1800 198 175 or by return email. You should also delete this email, and any copies, from your computer system network and destroy any hard copies produced. If not an intended recipient of this email, you must not copy, distribute or take any action(s) that relies on it; any form of disclosure, modification, distribution and/or publication of this email is also prohibited. Although Queensland Health takes all reasonable steps to ensure this email does not contain malicious software, Queensland Health does not accept responsibility for the consequences if any person's computer inadvertently suffers any disruption to services, loss of information, harm or is infected with a virus, other malicious computer programme or code that may occur as a consequence of receiving this email. Unless stated otherwise, this email represents only the views of the sender and not the views of the Queensland Government. **************************************************************** From brett_connolly <@t> merck.com Tue Dec 19 08:56:30 2006 From: brett_connolly <@t> merck.com (Connolly, Brett M) Date: Tue Dec 19 09:03:27 2006 Subject: [Histonet] bone tissue falling off after AR step Message-ID: <355C35514FEAC9458F75947F5270974D0145640B@usctmx1103.merck.com> Hey boneheads, A colleague is having problems with cortical bone detaching from the slide during or after the antigen retrieval step. He is working with mouse tibia. He uses Superfrost+ slides. The AR is with citrate buffer (pH6), performed in a waterbath for 10 minutes. He bakes the slides for 15 min at 56C prior to deparaffinizing. What do you suggest? Thanks, Brett Brett M. Connolly, Ph.D. Research Fellow MRL, Imaging Research Merck & Co., Inc. WP-44K PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com ------------------------------------------------------------------------------ Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ------------------------------------------------------------------------------ From pathrm35 <@t> charter.net Tue Dec 19 09:13:21 2006 From: pathrm35 <@t> charter.net (pathrm35@charter.net) Date: Tue Dec 19 09:13:33 2006 Subject: [Histonet] microwave processing on a budget... Message-ID: <1704042873.1166541201053.JavaMail.root@fepweb14> Tony, We use the Milestone microwave tissue processor for our about 40% of our skins cases with good results. We do 3 runs on the second shift with a max. of 112 cassettes in each run. The processing is good and consistent. I did have one issue where two pieces of tissue did become cooked though. The problem was that the magnetic spinner in the bottom of the glass jar became stuck underneith the plastic stand and wasn't moving the fluid around. This happens when you change the fluids and is caused by human error. I think a better design could help prevent this. When this happens the fluid isn't moving around and you get hot and cold spots in the fluid. All in all we have good results, better TAT and the MD's are happy. Ron Martin- --- Anthony Reilly wrote: > Hi Debbie > > I will be interested to hear other subscriber's comments with regard > to > microwave processing of skin. In our lab we do not have a microwave > processor but we do microwave fix on a regular basis. Having > said that we always resist microwave fixing skin as the process > damages > the skin tissue giving it that "overcooked" appearance which occurs > when > tissue has been exposed to too much heat during processing. This is a > > genuine artifact as we have found in a whole container of breast > tissue > which has been microwave fixed only the nipple will be damaged. > > With regard to your second hand processor, it is a bit like buying a > second > hand lawn mower, you get what you paid for. If it is a shandon from > circa > late 1980s early 1990s they were notoriously flawed, particularly the > heating bands for the retort.( Current models are fine) A new modern > standard processor will give you good morphology on small skins on a > 2-4 hour processing run and larger skins dependent on the amount of > fat > present will process well on 9-12 hour cycles. > > While microwave processors have their place in Histology for the high > throughput laboratories that are running 18-24 hours per day, if your > lab is working predominately day shifts only, I do not believe the > advantages of the microwave processors will be achieved and therefore > the extra cost of purchase is not warranted. As well as this most > microwave processors require the user to purchase the manufacturer's > "secret" proprietry solution/s creating further ongoing costs. > > regards > > > > > > Tony Reilly > Chief Scientist > Anatomical Pathology > QHPS-Prince Charles Hospital > Rode Rd Chermside Q 4032 > Australia > Ph: 07 3139 4543 > Fax: 07 3193 4546 > tony_reilly@health.qld.gov.au > > > >>> Debbie Keith 12/19/06 3:46 am >>> > Hey all... > > i have a few questions about microwave processing skin biopsies > utilizing a > microprocessor. i've looked into protocols and equipment but figured > this > would be the best/quickest sources of trial/error stories. > > i want to get a used lab microwave but want to hear good/bad stories > from > the folks in the trenches. :) marketers can sell me ANYTHING!! i > might be > the ONLY person that bought the magic bullet food processor AND the > ginsu > knife. (for the record, they are NOT as sharp after cutting through a > tin > can. fyi.) > > i purchased a used shandon hypercenter from a surplus lab equipment > place... and it's been such a NIGHTMARE. with enclosed processors > there > are so many possible issues. gaskets/circuit boards/cable ports.... > i'm > starting to think micro-processing would keep the biomedical-nightmares > to > a minimum. have i inhaled too many fumes or could i be onto something? > > i REALLY don't want to resort to an old carousel processor. > > the specimens will be limited to small shave/punch skin biopsies and > the > runs will never be more than 20 blocks. i would love to hear from > anyone > that has done this with good results. i'd like to hear the "avoid THIS > at > all costs...." stories as well. :) > > histonette is such a wonderful resource! you guys are AWESOME! > > debbie > > > -- > No virus found in this outgoing message. > Checked by AVG Free Edition. > Version: 7.1.409 / Virus Database: 268.15.24/592 - Release Date: > 12/18/2006 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ***************************************************************** > This email, including any attachments sent with it, is > confidential and for the sole use of the intended recipient(s). > This confidentiality is not waived or lost, if you receive it and > you are not the intended recipient(s), or if it is transmitted/ > received in error. > > Any unauthorised use, alteration, disclosure, distribution or > review of this email is strictly prohibited. The information > contained in this email, including any attachment sent with > it, may be subject to a statutory duty of confidentiality if it > relates to health service matters. > > If you are not the intended recipient(s), or if you have > received this email in error, you are asked to immediately > notify the sender by telephone collect on Australia > +61 1800 198 175 or by return email. You should also > delete this email, and any copies, from your computer > system network and destroy any hard copies produced. > > If not an intended recipient of this email, you must not copy, > distribute or take any action(s) that relies on it; any form of > disclosure, modification, distribution and/or publication of this > email is also prohibited. > > Although Queensland Health takes all reasonable steps to > ensure this email does not contain malicious software, > Queensland Health does not accept responsibility for the > consequences if any person's computer inadvertently suffers > any disruption to services, loss of information, harm or is > infected with a virus, other malicious computer programme or > code that may occur as a consequence of receiving this > email. > > Unless stated otherwise, this email represents only the views > of the sender and not the views of the Queensland Government. > **************************************************************** > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Tue Dec 19 09:48:27 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Dec 19 09:48:39 2006 Subject: [Histonet] bone tissue falling off after AR step In-Reply-To: <355C35514FEAC9458F75947F5270974D0145640B@usctmx1103.merck. com> References: <355C35514FEAC9458F75947F5270974D0145640B@usctmx1103.merck.com> Message-ID: <6.0.0.22.1.20061219084339.01b3cd48@gemini.msu.montana.edu> Brett, We drain the slide, then dry bone slides flat at 37 to 40C overnight and longer if possible. One bonehead expert years ago cracked the bone section drying whip and would not dry flat any less than two weeks but that is a timeline you may not like. You can then try going to a hotter oven for a short time, but we get more lift off of sections when the slides are dried in a hot oven. Be sure the bone sections are very flat after sectioning, and that these little tibias are very well infiltrated with paraffin. Good contact with the slide surface to begin with helps. At 07:56 AM 12/19/2006, you wrote: >Hey boneheads, >A colleague is having problems with cortical bone detaching from the >slide during or after the antigen retrieval step. He is working with >mouse tibia. > >He uses Superfrost+ slides. The AR is with citrate buffer (pH6), >performed in a waterbath for 10 minutes. He bakes the slides for 15 min >at 56C prior to deparaffinizing. > >What do you suggest? > >Thanks, Brett > >Brett M. Connolly, Ph.D. >Research Fellow >MRL, Imaging Research >Merck & Co., Inc. >WP-44K >PO Box 4 >West Point, PA 19486 >PH 215-652-2501 >fax. 215-993-6803 >e-mail. brett_connolly@merck.com > > >------------------------------------------------------------------------------ >Notice: This e-mail message, together with any attachments, contains >information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, >New Jersey, USA 08889), and/or its affiliates (which may be known >outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD >and in Japan, as Banyu - direct contact information for affiliates is >available at http://www.merck.com/contact/contacts.html) that may be >confidential, proprietary copyrighted and/or legally privileged. It is >intended solely for the use of the individual or entity named on this >message. If you are not the intended recipient, and have received this >message in error, please notify us immediately by reply e-mail and then >delete it from your system. > >------------------------------------------------------------------------------ >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From themagoos <@t> rushmore.com Tue Dec 19 10:20:23 2006 From: themagoos <@t> rushmore.com (themagoos) Date: Tue Dec 19 10:20:33 2006 Subject: [Histonet] microwave processing on a budget... Message-ID: <45881147.362.3cbf.76479696@rushmore.com> Ron, What does your program template look like? (Times, Temps, Watts. etc.)We are looking at micrwave processing skins also but we are having a hard time coming up with a program that will process skins well. Thank you for your input. Jason McGough HT(ASCP) Account Representative-Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th Street Suite 210 Rapid City, SD 57701 605-343-2267 jmcgough@clinlab.com ----- Original Message Follows ----- From: To: Anthony Reilly , histonet@lists.utsouthwestern.edu, debbiekeith@cox.net Subject: Re: [Histonet] microwave processing on a budget... Date: Tue, 19 Dec 2006 7:13:21 -0800 > Tony, > We use the Milestone microwave tissue processor for our > about 40% of our skins cases with good results. We do 3 > runs on the second shift with a max. of 112 cassettes in > each run. The processing is good and consistent. I did > have one issue where two pieces of tissue did become > cooked though. The problem was that the magnetic spinner > in the bottom of the glass jar became stuck underneith the > plastic stand and wasn't moving the fluid around. This > happens when you change the fluids and is caused by human > error. I think a better design could help prevent this. > When this happens the fluid isn't moving around and you > get hot and cold spots in the fluid. All in all we have > good results, better TAT and the MD's are happy. Ron > Martin- --- Anthony Reilly > > wrote: Hi Debbie > > > > I will be interested to hear other subscriber's comments > > with regard to > > microwave processing of skin. In our lab we do not have > > a microwave processor but we do microwave fix on a > > regular basis. Having said that we always resist > > microwave fixing skin as the process damages > > the skin tissue giving it that "overcooked" appearance > > which occurs when > > tissue has been exposed to too much heat during > > processing. This is a > > genuine artifact as we have found in a whole container > > of breast tissue > > which has been microwave fixed only the nipple will be > > damaged. > > With regard to your second hand processor, it is a bit > > like buying a second > > hand lawn mower, you get what you paid for. If it is a > > shandon from circa > > late 1980s early 1990s they were notoriously flawed, > > particularly the heating bands for the retort.( Current > > models are fine) A new modern standard processor will > > give you good morphology on small skins on a 2-4 hour > > processing run and larger skins dependent on the amount > > of fat present will process well on 9-12 hour cycles. > > > > While microwave processors have their place in Histology > > for the high throughput laboratories that are running > > 18-24 hours per day, if your lab is working > > predominately day shifts only, I do not believe the > advantages of the microwave processors will be achieved > > and therefore the extra cost of purchase is not > > warranted. As well as this most microwave processors > > require the user to purchase the manufacturer's "secret" > > proprietry solution/s creating further ongoing costs. > > regards > > > > > > > > > > > > Tony Reilly > > Chief Scientist > > Anatomical Pathology > > QHPS-Prince Charles Hospital > > Rode Rd Chermside Q 4032 > > Australia > > Ph: 07 3139 4543 > > Fax: 07 3193 4546 > > tony_reilly@health.qld.gov.au > > > > > > >>> Debbie Keith 12/19/06 3:46 am > > >>> Hey all... > > > > i have a few questions about microwave processing skin > > biopsies utilizing a > > microprocessor. i've looked into protocols and > > equipment but figured this > > would be the best/quickest sources of trial/error > > stories. > > i want to get a used lab microwave but want to hear > > good/bad stories from > > the folks in the trenches. :) marketers can sell me > > ANYTHING!! i might be > > the ONLY person that bought the magic bullet food > > processor AND the ginsu > > knife. (for the record, they are NOT as sharp after > > cutting through a tin > > can. fyi.) > > > > i purchased a used shandon hypercenter from a surplus > > lab equipment place... and it's been such a NIGHTMARE. > > with enclosed processors there > > are so many possible issues. gaskets/circuit > > boards/cable ports.... i'm > > starting to think micro-processing would keep the > > biomedical-nightmares to > > a minimum. have i inhaled too many fumes or could i be > > onto something? > > i REALLY don't want to resort to an old carousel > > processor. > > the specimens will be limited to small shave/punch skin > > biopsies and the > > runs will never be more than 20 blocks. i would love to > > hear from anyone > > that has done this with good results. i'd like to hear > > the "avoid THIS at > > all costs...." stories as well. :) > > > > histonette is such a wonderful resource! you guys are > > AWESOME! > > debbie > > > > > > -- > > No virus found in this outgoing message. > > Checked by AVG Free Edition. > > Version: 7.1.409 / Virus Database: 268.15.24/592 - > > Release Date: 12/18/2006 > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ********************************************************** > > ******* This email, including any attachments sent with > > it, is confidential and for the sole use of the intended > > recipient(s). This confidentiality is not waived or lost > > , if you receive it and you are not the intended > > recipient(s), or if it is transmitted/ received in > > error. > > Any unauthorised use, alteration, disclosure, > > distribution or review of this email is strictly > > prohibited. The information contained in this email, > > including any attachment sent with it, may be subject to > > a statutory duty of confidentiality if it relates to > > health service matters. > > If you are not the intended recipient(s), or if you have > > received this email in error, you are asked to > > immediately notify the sender by telephone collect on > > Australia +61 1800 198 175 or by return email. You > > should also delete this email, and any copies, from your > > computer system network and destroy any hard copies > > produced. > > If not an intended recipient of this email, you must not > > copy, distribute or take any action(s) that relies on it > > ; any form of disclosure, modification, distribution > > and/or publication of this email is also prohibited. > > > > Although Queensland Health takes all reasonable steps to > > ensure this email does not contain malicious software, > > Queensland Health does not accept responsibility for the > > consequences if any person's computer inadvertently > > suffers any disruption to services, loss of information, > > harm or is infected with a virus, other malicious > > computer programme or code that may occur as a > > consequence of receiving this email. > > > > Unless stated otherwise, this email represents only the > > views of the sender and not the views of the Queensland > > Government. > ********************************************************** > > ****** > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Tue Dec 19 11:25:48 2006 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Tue Dec 19 11:26:04 2006 Subject: [Histonet] frozen tissue organization Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6F21@EMAIL.archildrens.org> Our frozen tissue bank is a mess. I am trying to organize this project and I am looking for a way to store frozen tissue samples so they can be organized and easily retrieved. How do others handle this problem? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From sbreeden <@t> nmda.nmsu.edu Tue Dec 19 11:34:30 2006 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Dec 19 11:34:41 2006 Subject: [Histonet] Used Leica 1512 Price? Message-ID: <4D14F0FC9316DD41972D5F03C070908B0DB3AC@nmdamailsvr.nmda.ad.nmsu.edu> Would anyone have an estimated price for a used Leitz 1512 microtome? This would be a backup/parts unit. Before I go looking for one, I'd like to know how much one would be worth. Dear vendors: I'm not in a position to buy a new microtome right now and ask that you not assume that I would like pricing for same - perhaps when we win the lottery, but now I'm just looking for "ballpark" pricing specifically for a LEITZ 1512. Thanks, everyone, in advance. And - surprise, surprise! It's snowing in Albuquerque! Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 From gu.lang <@t> gmx.at Tue Dec 19 13:05:06 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Dec 19 13:03:50 2006 Subject: [Histonet] microwave processing Message-ID: <004001c723a0$993d4f20$c812a8c0@dielangs.at> I've read the interesting discussion about microwave processors and have a general question. Nowadays there is the demand of standardization in histolabs. From tissue to tissue and from lab to lab we have to minimize the differences in processing the specimen. So tests like immunohistochemistry can be performed in high standards and reliance. How do labs match this issue when introducing a new technology like microwave processors? Do they use old and new processors parallel? Can you run all sorts of tissue with it? If this isn't possible, are there two "kinds" of paraffin-blocks to make fitting protocols? What about these FDA-proved tests? After all the work you have the lab-own standards, but aren't "allowed" to do the official test? I think about biopsies, that are partly proceeded in microwaver, partly in the old instrument over night, specimen fixed in formalin for few hours up to a week, or fixed in a new reagens without formalin,.... Perhaps someone could share her/his experience with us. Gudrun Lang From rjbuesa <@t> yahoo.com Tue Dec 19 13:03:52 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Dec 19 13:04:00 2006 Subject: [Histonet] frozen tissue organization In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6F21@EMAIL.archildrens.org> Message-ID: <525499.11716.qm@web61212.mail.yahoo.com> I used to store the individual specimens in small plastic bags (2" x 3") with a label inside (visible by transparency). These bags were placed in rows in small cardoard boxes. The boxes were identified with the sequence of specimens numbers. Each carboard box had a cover. No more than 100 specimens per box. Ren? J. "Horn, Hazel V" wrote: Our frozen tissue bank is a mess. I am trying to organize this project and I am looking for a way to store frozen tissue samples so they can be organized and easily retrieved. How do others handle this problem? Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From rjbuesa <@t> yahoo.com Tue Dec 19 13:31:42 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Dec 19 13:31:51 2006 Subject: [Histonet] microwave processing In-Reply-To: <004001c723a0$993d4f20$c812a8c0@dielangs.at> Message-ID: <462797.85342.qm@web61225.mail.yahoo.com> Gudrun: Unfortunately there is not much standardization in histology, except for, as you point out, some FDA approved tests, like Her2Neu where almost all labs do it in the same way, specially because manufacturers like DAKO that even provide free steamers and water baths, to assure that the tests (done with their products) are run in a standardized manner. Appart from this exception any histology lab is run pretty much as it has been run since its start or since the present supervisor "started to take care of (and modify!) things". Overnight protocols vary in overall and/or in stations duration; antibody dilutions for IHC vary from lab to lab for the same antibody and also some preffer one detection system over another; automatic staining also "suffers" from the same interlab differences; the reagents and stains are different, and some are "home made" and others bought ; the fixatives also vary and fixation is different. You name the step, and you will find that each lab "holds its ground" to "deffend" its procedures and protocols without any scientific basis, except for "preference" or "I have done it like this always". This is unfortunate, but is a fact of life in histology. The thing is that since histology is still mainly a qualitative activity, it does not matter that much (except for some extreme affecting antigens integrity). We process tissue and prepare slides that are going to be used in a qualitative manner, that are going to be used to identify a structural pattern, not to quantify things. This is why histology is such a special area of the medical lab and will continue that way until molecular pathology does not take hold of the entire operation, until it will be necessary to preserve DNA and RNA and formalin will be substituted, probably after very "strong fights" dealing with its "universality" and "usefulness". Under separate cover I am sending you an article that has been accepted for publication in the Feb./07 issue of Annal of Diagnostic Pathology that deals with these facts. I hope I have not added more confusion to the issue! Ren? J. Gudrun Lang wrote: I've read the interesting discussion about microwave processors and have a general question. Nowadays there is the demand of standardization in histolabs. From tissue to tissue and from lab to lab we have to minimize the differences in processing the specimen. So tests like immunohistochemistry can be performed in high standards and reliance. How do labs match this issue when introducing a new technology like microwave processors? Do they use old and new processors parallel? Can you run all sorts of tissue with it? If this isn't possible, are there two "kinds" of paraffin-blocks to make fitting protocols? What about these FDA-proved tests? After all the work you have the lab-own standards, but aren't "allowed" to do the official test? I think about biopsies, that are partly proceeded in microwaver, partly in the old instrument over night, specimen fixed in formalin for few hours up to a week, or fixed in a new reagens without formalin,.... Perhaps someone could share her/his experience with us. Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From gu.lang <@t> gmx.at Tue Dec 19 14:18:42 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Dec 19 14:17:58 2006 Subject: [Histonet] AW: Histology: a unique area of the medical laboratory. In-Reply-To: <20061219193439.36664.qmail@web61211.mail.yahoo.com> Message-ID: <005601c723aa$e33fcc10$c812a8c0@dielangs.at> Thank you Ren?, You always show me, that you are a great ?thinker? about our profession. You are right, that everybody makes his own soup. And I cannot except myself when you refer to defending the own protocols. But I think the scala of handling the specimen has become smaller. Especially with diagnostic labs, we use nearly the same processors, more and more ready-made reagens, the results are comparable. ? on the basic of formalin fixing, dehydrating and wax-embedding. The instruments are aimed on the FFPE-tissue, so I?m interested in experiences of people with new technologies. And merry Christmas to you and your familiy, too! Gudrun Lang Biomed. Analytikerin Histolabor Akh Linz Krankenhausstr. 9 4020 Linz +43(0)732/7806-6754 _____ Von: Rene J Buesa [mailto:rjbuesa@yahoo.com] Gesendet: Dienstag, 19. Dezember 2006 20:35 An: Gudrun Lang Betreff: Histology: a unique area of the medical laboratory. Dear Gudrun: This is the article I wrote you about. Merry Christmas and Happy 2007 to you and your family! Ren? J. __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From Tracey.Lenek <@t> CLS.ab.ca Tue Dec 19 14:42:27 2006 From: Tracey.Lenek <@t> CLS.ab.ca (Tracey.Lenek@CLS.ab.ca) Date: Tue Dec 19 14:43:56 2006 Subject: [Histonet] Automated Special Stainers Message-ID: <4BC300747AF87A48BCDF8E48BC2885CE040BB4AF@mail1.calgary.com> Hello everyone, I know this question has been posted before - but we are interested in the feedback for both the "Artisan" from Dako and Ventana's Special Stainer. We have just demo'd both and were wondering what the general consensus is? Thanks in advance. Tracey CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. From rjbuesa <@t> yahoo.com Tue Dec 19 14:56:55 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Dec 19 14:57:04 2006 Subject: [Histonet] Automated Special Stainers In-Reply-To: <4BC300747AF87A48BCDF8E48BC2885CE040BB4AF@mail1.calgary.com> Message-ID: <219160.82818.qm@web61219.mail.yahoo.com> This answer has also been posted many times, but it does not hurt to post it again: everything "boils down" to which system you prefer: OPEN (Dako) or CLOSED (Ventana). One that allows you to use any reagent and protocol or not. I prefer DAKO! Ren? J. Tracey.Lenek@CLS.ab.ca wrote: Hello everyone, I know this question has been posted before - but we are interested in the feedback for both the "Artisan" from Dako and Ventana's Special Stainer. We have just demo'd both and were wondering what the general consensus is? Thanks in advance. Tracey CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From JGREWE <@t> OhioHealth.com Tue Dec 19 15:13:54 2006 From: JGREWE <@t> OhioHealth.com (JGREWE@OhioHealth.com) Date: Tue Dec 19 15:14:10 2006 Subject: [Histonet] Jacquelyn Grewe/Staff/OhioHealth is out of the office . Message-ID: I will be out of the office starting 12/19/2006 and will not return until 01/02/2007. I will respond to your message when I return. Thanks, Jackie From godsgalnow <@t> aol.com Tue Dec 19 15:32:08 2006 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Tue Dec 19 15:32:20 2006 Subject: [Histonet] microcosting Message-ID: <8C8F1CC451DC5CE-A44-169@FWM-D30.sysops.aol.com> Anyone out there willing to share your spreadsheet for microcosting routine tissue processing? I would greatly appreciate it. I have been hitting my head against the wall on this for weeks; everytime I do it, it comes out different. I need a guideline. Thank you so much Roxanne Soto HT(ASCP)QIHC ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From gcallis <@t> montana.edu Tue Dec 19 16:46:05 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Dec 19 16:46:18 2006 Subject: [Histonet] Happy Holidays to Histonetters Message-ID: <6.0.0.22.1.20061219154128.01b0acc8@gemini.msu.montana.edu> Dear Histonetters, Happy Holidays to this delightful international group of histonetters! "See" you in 2007--------- Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 From Robin.Barrett <@t> vision-bio.com Tue Dec 19 18:03:43 2006 From: Robin.Barrett <@t> vision-bio.com (Robin Barrett) Date: Tue Dec 19 18:03:54 2006 Subject: [Histonet] Happy Holidays to Histonetters Message-ID: I Robin Barrett 832.729.5400 _________________ Cingular BlackBerry ----- Original Message ----- From: histonet-bounces@lists.utsouthwestern.edu To: Histonet@lists.utsouthwestern.edu Sent: Tue Dec 19 17:46:05 2006 Subject: [Histonet] Happy Holidays to Histonetters Dear Histonetters, Happy Holidays to this delightful international group of histonetters! "See" you in 2007--------- Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tahseen <@t> brain.net.pk Tue Dec 19 18:38:16 2006 From: tahseen <@t> brain.net.pk (Tahseen) Date: Tue Dec 19 18:38:18 2006 Subject: [Histonet] microcosting References: <8C8F1CC451DC5CE-A44-169@FWM-D30.sysops.aol.com> Message-ID: <001d01c70c3c$08965730$b21480cb@piii> See Tech Sample HT-1 (1996) Cost Accounting In Histology Department. Muhammad Tahseen Histology Supervisor, SKMCH&RC Lahore,Pakistan. ----- Original Message ----- From: To: Sent: Wednesday, December 20, 2006 2:32 AM Subject: [Histonet] microcosting > Anyone out there willing to share your spreadsheet for microcosting > routine tissue processing? I would greatly appreciate it. I have been > hitting my head against the wall on this for weeks; everytime I do it, it > comes out different. I need a guideline. > > Thank you so much > > Roxanne Soto HT(ASCP)QIHC > > > > ________________________________________________________________________ > Check out the new AOL. Most comprehensive set of free safety and security > tools, free access to millions of high-quality videos from across the web, > free AOL Mail and more. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> heart.thi.tmc.edu Wed Dec 20 05:49:26 2006 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Wed Dec 20 05:49:35 2006 Subject: [Histonet] GMA/PSR Message-ID: Happy holidays! Does anyone have a Picro Sirius Red protocol for GMA sections? Thank you. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) From Karen.Heckford <@t> CHW.edu Wed Dec 20 06:54:45 2006 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Wed Dec 20 06:55:43 2006 Subject: [Histonet] Prostate Biopsy's Message-ID: Happy Holidays Everyone, I would like to get 40 Prostate needle biopsies out of the way this mornning. Does anyone have a short processing protocol. I have a Shandon Excelsior. I was thinking about 15 minutes per station. Do you think I can get away with less time? Feel Free to call or email back ASAP. Cheers, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathology Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu From ryakay <@t> shands.ufl.edu Wed Dec 20 06:56:07 2006 From: ryakay <@t> shands.ufl.edu (Kaye Ryan) Date: Wed Dec 20 06:56:43 2006 Subject: [Histonet] Automated Special Stainers Message-ID: Hi Tracey, We have two Artisan stainers in our lab and have been utilizing them for almost 2 years now. We love them. They are consistent from stainer to stainer and very easy to use. You just have to practice your grouping of stains to utilize them to their fullest. We have had very few problems with them or the kits. Kaye Ryan Histology Manager/Educational Coordinator Rocky Point Laboratories 265-0111, 72093 >>> 12/19/2006 3:42 PM >>> Hello everyone, I know this question has been posted before - but we are interested in the feedback for both the "Artisan" from Dako and Ventana's Special Stainer. We have just demo'd both and were wondering what the general consensus is? Thanks in advance. Tracey CONFIDENTIALITY NOTICE: This e-mail and any files/attachments may contain confidential, personal and/or privileged information intended for a specific purpose and recipient. If you are not the intended recipient do not disclose, copy, retain, distribute, use or modify any of the contents of this transmission. If you received this transmission in error please notify me immediately by return e-mail or telephone and destroy the entire transmission and any copies produced. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Wed Dec 20 07:23:13 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Dec 20 07:23:22 2006 Subject: [Histonet] microcosting In-Reply-To: <8C8F1CC451DC5CE-A44-169@FWM-D30.sysops.aol.com> Message-ID: <493810.74202.qm@web61223.mail.yahoo.com> Hi Roxane: There are different ways of calculating to costs. One is an "unitary" approach where you try to calculate the cost of a single run, operation or procedure. This is too much time consuming and prone to great fluctuations because almost every run if different to the others and probably will not require renew all the used reagents. The other is a "bulk" approach where you keep track of all expendables used during a period of time, either a week, a month or a year. I prefer this type of approach and regularly used the month averages.For example: if you keep track of all the reagents in your H&E stainer during 1 week, and know how many slides you stained, the total cost of the automatic stainer reagents divided by the total number of slides stained = cost per slide.You do the same for the cost of the slides, and that of the automatic coverslipper and you will find out the cost of preparing 1 H&E slide. If you at the same time keep track of the cost of the reagents for the tissue processors and divided by the number of cassettes processed = cost of 1 cassette. And you keep doing this along the workflow = cost of 1 slide from reception of the specimen to the slide ready for the pathologist. To do it keep track of all the specimens processed, slides produced, everything you want to calculate the cost of and all the amounts and costs of all expendables required to complete those tasks. The total costs or amounts of expendables divided by the total number of completed items will give you the unitary cost, and it will be an average figure, where the variations that existed are going to be "even" out. If you combine these costs to the productivity figures of your lab (how much units of work per unit of time per HT) you will have the LABOR cost. At the end material costs + labor costs = total cost per unit. During the las NSH Meeting (2006) there was a workshop about costs that provided many forms to fill and had very useful information. Try to get a copy of that workshop. I am also attaching under separate cover a paper of mine that will appear in the Jan.5/07 issue of Advance for MLP". Hope this will help you! Ren? J. godsgalnow@aol.com wrote: Anyone out there willing to share your spreadsheet for microcosting routine tissue processing? I would greatly appreciate it. I have been hitting my head against the wall on this for weeks; everytime I do it, it comes out different. I need a guideline. Thank you so much Roxanne Soto HT(ASCP)QIHC ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From histology.bc <@t> shaw.ca Wed Dec 20 10:56:47 2006 From: histology.bc <@t> shaw.ca (Paul Bradbury) Date: Wed Dec 20 11:01:01 2006 Subject: [Histonet] Von Kossa stain In-Reply-To: <002701c7227e$21949dc0$ba3a4ea0@PC186Gabbi> References: <002701c7227e$21949dc0$ba3a4ea0@PC186Gabbi> Message-ID: <45896B4F.9060405@shaw.ca> Many years ago, I also did some work with MMA sections of undecalcified bone. I encountered much the same problems that you are experiencing. So, a couple of suggestions: To make the mineralized portions of the bone black (rather than brown), treat the sections with silver nitrate as in the regular von Kossa technique, wash well in distilled water and then treat the sections with 0.5% aqueous Hydroquinone. This is a good reducing agent and will convert the silver to a stable black metallic form. The section must be well washed before this step or you will get all kinds of ugly precipitates. If you have washed really well, you can usually omit the thiosulphate step as all it does is to reduce the contrast of the silver. Once you have good dense black calcium deposits , you can counterstain with a van Gieson (picric acid and acid fuchsin). This will give beautiful bright red osteoid seams, and most of the bone marrow will be yellow. You may have to play around with the timing of the van Gieson to get optimal results. But both dyes are quite small molecules and should penetrate quite fast; I would start at around 2 minutes and see how it looks. Slow dehydration will also remove a lot of the red and yellow, so don't spend too long in the dehydrating alcohols. Paul Bradbury Kamloops, BC Canada Brunella Spaggiari wrote: >Hello everybody, > >we work on undelcalcified bone tissue (human, rabbit) fixed in PFA 4% and embedded in methylmethacrilate (MMA). >>From our samples we obtain 50 ?m thick sections which can contain titanium implants. >We tried to perform Von Kossa stain, always on undeplasticized sections, but we met several problems: > >- calcium deposits after silver nitrate+UV light did not appear black but brown, and, after sodium thiosulphate, brown stain turns more and more light; > >- we could not find a suitable counterstain for osteoid: nuclear fast red and neutral red do not stain at all, basic or acid fuchsin stain too much and cover the underlying brown.. > >Some suggestions? Are there other good histological stainings for undeplasticized bone sections in your experience? > >Thank you! >Brunella >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > From jcline <@t> wchsys.org Wed Dec 20 12:13:58 2006 From: jcline <@t> wchsys.org (Joyce Cline) Date: Wed Dec 20 12:14:07 2006 Subject: [Histonet] Special Stainers Message-ID: <000c01c72462$9ea72ea0$1d2a14ac@wchsys.org> I have used the Ventana Special Stainer since 1998 and we love it. Occasionally there is a glitch but the service is great. The technical time saved with a special stainer is well worth the expense. For those of us who can not get people replaced, it is really worth it. ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From mtitford <@t> aol.com Wed Dec 20 12:22:41 2006 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Wed Dec 20 12:22:59 2006 Subject: [Histonet] Christmas greetings and thanks to: Message-ID: <8C8F27AF8D0EF38-1750-79AF@FWM-D12.sysops.aol.com> Christmas greetings to everyone from the Heart of Dixie, and thanks to Dr Linda Margraf and others for hosting the "Histonet" Mike Titford Pathology USA Mobile AL ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From pwebster <@t> swmail.sw.org Wed Dec 20 14:06:46 2006 From: pwebster <@t> swmail.sw.org (Patricia Webster) Date: Wed Dec 20 14:07:02 2006 Subject: [Histonet] Looking for someone Message-ID: I have lost contact with two friends (HT's) over the years and was wondering if anyone could help me find them. Kathy Moore - Last know residence - Arizona Paula Williams - " " - Alaska Thanks, Trish Webster From opiecurt <@t> yahoo.com Wed Dec 20 14:10:15 2006 From: opiecurt <@t> yahoo.com (curt tague) Date: Wed Dec 20 14:10:22 2006 Subject: [Histonet] southern california pay question Message-ID: <20061220201015.89008.qmail@web81613.mail.mud.yahoo.com> currently i have 2 trainees but may be looking to add/replace soon and i am curious as to what the market is dictating for experienced and/or trainee techs. maybe someone has a link or infomation regarding the pay scale in southern california. thanks, curt __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From JWEEMS <@t> sjha.org Wed Dec 20 14:15:18 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Wed Dec 20 14:16:00 2006 Subject: [Histonet] Cat Scratch (Bartonella) Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D2D3E@sjhaexc02.sjha.org> We have a clinician requesting more stains on a small specimen. (Our Warthin Starry was negative). What would you all recommend for the best results and could I send this to someone for testing? Many thanks in advance, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From pruegg <@t> ihctech.net Wed Dec 20 15:17:41 2006 From: pruegg <@t> ihctech.net (patsy ruegg) Date: Wed Dec 20 15:17:50 2006 Subject: [Histonet] bone tissue falling off after AR step In-Reply-To: <355C35514FEAC9458F75947F5270974D0145640B@usctmx1103.merck.com> Message-ID: <200612202117.kBKLHYmD058136@pro12.abac.com> Brett, Bone is hard and the cortical bone will be the most difficult to preserve. For IHC on bone I airdry the sections overnight before baking at 58dc for at least 1 hr. I try to avoid HIER and use enzyme digestion if possible for antigen retrieval. If I do need to use HIER, I use a waterbath no higher than 72dc for 3 hrs. or 32dc overnight. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Connolly, Brett M Sent: Tuesday, December 19, 2006 7:57 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] bone tissue falling off after AR step Hey boneheads, A colleague is having problems with cortical bone detaching from the slide during or after the antigen retrieval step. He is working with mouse tibia. He uses Superfrost+ slides. The AR is with citrate buffer (pH6), performed in a waterbath for 10 minutes. He bakes the slides for 15 min at 56C prior to deparaffinizing. What do you suggest? Thanks, Brett Brett M. Connolly, Ph.D. Research Fellow MRL, Imaging Research Merck & Co., Inc. WP-44K PO Box 4 West Point, PA 19486 PH 215-652-2501 fax. 215-993-6803 e-mail. brett_connolly@merck.com ---------------------------------------------------------------------------- -- Notice: This e-mail message, together with any attachments, contains information of Merck & Co., Inc. (One Merck Drive, Whitehouse Station, New Jersey, USA 08889), and/or its affiliates (which may be known outside the United States as Merck Frosst, Merck Sharp & Dohme or MSD and in Japan, as Banyu - direct contact information for affiliates is available at http://www.merck.com/contact/contacts.html) that may be confidential, proprietary copyrighted and/or legally privileged. It is intended solely for the use of the individual or entity named on this message. If you are not the intended recipient, and have received this message in error, please notify us immediately by reply e-mail and then delete it from your system. ---------------------------------------------------------------------------- -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jnocito <@t> satx.rr.com Thu Dec 21 07:53:15 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Dec 21 07:53:30 2006 Subject: [Histonet] Prostate Biopsy's References: Message-ID: <00a201c72507$5d2cbbc0$31614542@yourxhtr8hvc4p> Karen, I skip the formalins if the bx's are well fixed, 10 minutes each in the alcohols, 9 minutes each in the xylenes and paraffins. I do not use a xylene substitute. You might have to increase the clearing time if you do. Happy Holidays Joe Nocito ----- Original Message ----- From: "Heckford, Karen - SMMC-SF" To: "Histonet (E-mail)" Sent: Wednesday, December 20, 2006 6:54 AM Subject: [Histonet] Prostate Biopsy's > Happy Holidays Everyone, I would like to get 40 Prostate needle biopsies > out of the way this mornning. Does anyone have a short processing > protocol. > I have a Shandon Excelsior. I was thinking about 15 minutes per station. > Do you think I can get away with less time? > Feel Free to call or email back ASAP. > Cheers, > Karen Heckford HT (ASCP) CE > Lead Histology Technician > Histology/Pathology Department > St. Mary's Medical Center > 450 Stanyan St. > San Francisco, Ca. 94117 > 415-668-1000 ext. 6167 > Fax: 415-750-8123 > email: kheckfor@chw.edu > -------------------------------------------------------------------------------- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From dpahisto <@t> yahoo.com Thu Dec 21 08:17:00 2006 From: dpahisto <@t> yahoo.com (Cindy DuBois) Date: Thu Dec 21 08:17:11 2006 Subject: [Histonet] Pott's Stain Message-ID: <901506.40571.qm@web33402.mail.mud.yahoo.com> Is anyone using this procedure for staining multiple organisms at the same time on one slide? If so, How do you like it? .... Is it worth the extra time vs. staining multiple slides for each individual component (fungus, pneumocystis & mycobacterium) at once? How are you running a control? Happy Holidays, and thank you, Cindy DuBois Integrated Pathology Stockton, CA __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From skgoud69 <@t> yahoo.co.in Thu Dec 21 09:18:12 2006 From: skgoud69 <@t> yahoo.co.in (sanjeev kumar) Date: Thu Dec 21 09:18:29 2006 Subject: [Histonet] C4d Message-ID: <199343.82171.qm@web7710.mail.in.yahoo.com> Dear all, I am getting a lot of background staining while doing immunohistochemistry markers of immunoglobulins on paraffin blocks, specially C4d. Can anybody help me which companys antibody will be the better and please mail me the protocol . thank you all Sanju Care Hospital Hyderabad India Send free SMS to your Friends on Mobile from your Yahoo! Messenger. Download Now! http://messenger.yahoo.com/download.php From BMolinari <@t> heart.thi.tmc.edu Thu Dec 21 09:48:21 2006 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Thu Dec 21 09:48:29 2006 Subject: [Histonet] PSR/trichrome in paraffin Message-ID: Hi again, PSR seems to be the order of the week. This involves paraffin sections. PSR is more specific for collagen but the investigator wants to know which shows the difference between "mature" and "immature" collagen. She is trying to do quantitative analysis and is seeing larger areas of collagen in the trichrome slides. Thanks. Betsy Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) From pkarlisch <@t> psu.edu Thu Dec 21 10:40:46 2006 From: pkarlisch <@t> psu.edu (Patricia Karlisch) Date: Thu Dec 21 10:41:23 2006 Subject: [Histonet] Question/ Help Message-ID: <458A72BE0200008C000336C6@GWIA02.HERSHEYMED.NET> All, We are having a intermittent problem with our small biopsies. Even though most are run on one dedicated short run processing cycle we have artifact staining problems. Sometimes they are very dry. We can handle those. Sometimes the tissue is fixed and stained half way and the rest shows NO nuclear detail. A while back we could send out slides to a lab and they would look at the slide and determine the best method of avoiding processing problems. Does anyone still do this?? We have a number of slides that we would like someone else to review as far as artifact staining. Thank you in advance. Pat Karlisch Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. From LSebree <@t> uwhealth.org Thu Dec 21 10:57:26 2006 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Thu Dec 21 10:57:34 2006 Subject: [Histonet] C4d In-Reply-To: <199343.82171.qm@web7710.mail.in.yahoo.com> Message-ID: What kind of tissue are you doing your C4ds on? Linda Sebree, HT(ASCP) University of Wisconsin Hospital & Clinics IHC/ISH Laboratory A4/204-3224 600 Highland Ave. Madison, WI 53792 (608)265-6596 FAX: (608)262-7174 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sanjeev kumar Sent: Thursday, December 21, 2006 9:18 AM To: histonet@lists.utsouthwestern.edu; histonet@histosearch.com Subject: [Histonet] C4d Dear all, I am getting a lot of background staining while doing immunohistochemistry markers of immunoglobulins on paraffin blocks, specially C4d. Can anybody help me which companys antibody will be the better and please mail me the protocol . thank you all Sanju Care Hospital Hyderabad India Send free SMS to your Friends on Mobile from your Yahoo! Messenger. Download Now! http://messenger.yahoo.com/download.php _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Thu Dec 21 11:59:11 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Dec 21 11:59:35 2006 Subject: [Histonet] Cat Scratch (Bartonella) In-Reply-To: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D2D3E@sjhaexc02.sjha.org> References: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D2D3E@sjhaexc02.sjha.org> Message-ID: <458A851F020000770000364C@hcnwgwds01.hh.chs> My laboratory does IHC for Bartonella henselae. We have extensive experience with this test. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Weems, Joyce" 12/20/06 3:15 PM >>> We have a clinician requesting more stains on a small specimen. (Our Warthin Starry was negative). What would you all recommend for the best results and could I send this to someone for testing? Many thanks in advance, Joyce Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. Confidentiality Notice This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential or proprietary information which is legally privileged. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please promptly contact the sender by reply e-mail and destroy all copies of the original message. From debbiekeith <@t> cox.net Thu Dec 21 13:00:00 2006 From: debbiekeith <@t> cox.net (Debbie Keith) Date: Thu Dec 21 13:00:12 2006 Subject: [Histonet] anyone have the Microm 312/316 carousel stainers? Message-ID: <5.2.0.9.0.20061221115842.0335b6d8@pop.central.cox.net> i am interested in getting one and was wondering if anyone has one.... likes/hates it? it looks like a great machine!!! debbie -- No virus found in this outgoing message. Checked by AVG Free Edition. Version: 7.1.409 / Virus Database: 268.15.26/594 - Release Date: 12/20/2006 From jcline <@t> wchsys.org Thu Dec 21 13:13:50 2006 From: jcline <@t> wchsys.org (Joyce Cline) Date: Thu Dec 21 13:13:59 2006 Subject: [Histonet] Special Stainers/Jeanine In-Reply-To: Message-ID: <000c01c72534$26287060$1d2a14ac@wchsys.org> The special stains can be tweaked using different protocols ex: GMS light or dark, NFR light or dark, Pn.Cys. light or dark staining. I tried different protocols until the pathologist found the one he wanted. -----Original Message----- From: Bartlett, Jeanine (CDC/CCID/NCZVED) [mailto:jqb7@cdc.gov] Sent: Wednesday, December 20, 2006 1:31 PM To: Joyce Cline Subject: RE: [Histonet] Special Stainers Are you using the NexES? And do you have the ability to teak the stains if needed? Thanks, Jeanine Bartlett, BS, HT(ASCP) Centers for Disease Control and Prevention 1600 Clifton Road, MS/G-32 18/SB-114 Atlanta, GA 30333 (404) 639-3590 jeanine.bartlett@cdc.hhs.gov -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joyce Cline Sent: Wednesday, December 20, 2006 1:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Special Stainers I have used the Ventana Special Stainer since 1998 and we love it. Occasionally there is a glitch but the service is great. The technical time saved with a special stainer is well worth the expense. For those of us who can not get people replaced, it is really worth it. ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From SBaldwin <@t> compucyte.com Thu Dec 21 13:28:05 2006 From: SBaldwin <@t> compucyte.com (Scott Baldwin) Date: Thu Dec 21 14:02:51 2006 Subject: [Histonet] Cold Spring Harbor Symposium Message-ID: To all Northeast area Histonetters and other interested parties, Cold Spring Harbor Laboratory is hosting our annual symposium on Quantitative Imaging Cytometry, to be held in the Woodbury Auditorium on the CSHL Woodbury campus, on Tuesday, February 20. Since there are a number of presentations involving tissue analysis applications, tissue microarrays, quantitative Immunohistochemistry and Pathology applications, I thought this might be of interest to the group. The presentations include: * "Cellular Life as a Biochemical Finite State Machine: Implications for Cell Based Assays" - James Jacobberger, PhD, Professor of Oncology, Associate Director for Shared Resources, Director Cytometry Core, Case Comprehensive Cancer Center, Cleveland OH "Activation of ATM and phosphorylation of H2AX reporters of DNA damage" - Zbigniew Darzynkiewicz. MD, PhD, Brander Cancer Research Institute, New York Medical College, Valhalla, NY * "An introduction to Laser Scanning Cytometry: Individuals Cells and Whole Tissues" (LSC technology and applications update, with focus on apoptosis and tissue analysis) William Telford, PhD, Core Flow Cytometry, NIH/NCI, Bethesda, MD * "Pharmacodynamic monitoring of molecular cancer therapeutics at the preclinical and early clinical stages of development" - David W Hedley, MD, Dept. of Medical Oncology and Hematology, Division of Experimental Therapeutics, Princess Margaret Hospital/Ontario Cancer Institute, University of Toronto * "An automated method to monitor skin mast cells by Laser Scanning Cytometry" Gloria Juan, Sr. Scientist, Clinical Immunology, Amgen * "LSC analysis of tissue microarrays: Application to subcellular localization of p27 and prostate cancer recurrence" - Peter Gann, MD, ScD, Department of Pathology, College of Medicine, University of Illinois at Chicago * "Quantitative Imaging Cytometry: Technology to support the new role of pathology in the era of specific targeted therapies"- William Geddie, MD, Department of Laboratory Medicine and Pathobiology, University of Toronto We will also offer an iCys? Research Imaging Cytometer hands-on clinic where we will make our Flow Cytometry Core instrument available for demonstration by CompuCyte scientists, who will also accept samples for evaluation on the instrument. This session will be held the following day, February 21, at the Flow Cytometry Core Facility in the Hershey Building on the Cold Spring Harbor Campus. All scientists interested in evaluating their samples must make prior arrangements with the organizers. A more detailed agenda and registration materials are available at www.compucyte.com . Registration for the conference is free of charge, but is necessary if you wish to have lunch. You obtain more information by contacting Pam Moody at moody@cshl.edu . I hope this is of interest! HAPPY HOLIDAYS to everyone! Scott Baldwin MT(ASCP) From Shirley_PHUA <@t> hsa.gov.sg Thu Dec 21 14:05:18 2006 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Thu Dec 21 14:12:34 2006 Subject: [Histonet] Shirley is away 22 December 2006 Message-ID: I will be out of the office from 22-12-2006 to 25-12-2006. I will return on 25 December 2006 Pathologists : I will process your requests when I return. Otherwise, if urgent, please forward your mail to henry_kyaw@hsa.gov.sg Regrets for the inconveniences caused. From heupel <@t> zeiss.de Thu Dec 21 15:06:27 2006 From: heupel <@t> zeiss.de (Heupel, Thorsten) Date: Thu Dec 21 15:03:56 2006 Subject: [Histonet] Dr. Thorsten Heupel ist =?iso-8859-1?q?au=DFer_Haus_/_Dr=2E_Thors?= =?iso-8859-1?q?ten_Heupel_is_out_of_office?= Message-ID: Ich werde ab 21.12.2006 nicht im B?ro sein. Ich kehre zur?ck am 04.01.2007. Ich werde Ihre Nachrichten nach meiner R?ckkehr beantworten. Mit freundlichen Gr??en Thorsten Heupel I am out of the office from 12/21/2006 until 01/03/2007 I will respond to your message after my return. Best regards, Thorsten Heupel ________________________________________ Carl Zeiss MicroImaging GmbH Abteilung MI-VS / Department MI-VS Applikationsspezialist / Application Specialist D r. T h o r s t e n H e u p e l Telefon/Phone: +49 3641 64-2677 Fax: +49 3641 64-3144 mailto: heupel@zeiss.de http://www.zeiss.de/micro From jdhisto <@t> yahoo.com Fri Dec 22 06:50:46 2006 From: jdhisto <@t> yahoo.com (Jonathan De La Rosa) Date: Fri Dec 22 06:50:55 2006 Subject: [Histonet] fall off sections Message-ID: <855283.60155.qm@web36213.mail.mud.yahoo.com> HELLO, I'M HAVING A SECTION (BREAST) THAT HAS FALLEN OFF SEVERAL TIMES AFTER IHC RUN. THIS HAS BEEN ATTEMPTED 4 TIMES AND IS THE FIRST TIME IVE EXPERIENCED IT TO THIS DEPT. ANY SUGGESTIONS ? Jonathan N. De La Rosa HT New Braunfels, TX 78130 210-412-5837 JDhisto@yahoo.com From rjbuesa <@t> yahoo.com Fri Dec 22 08:42:05 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Dec 22 08:42:14 2006 Subject: [Histonet] fall off sections In-Reply-To: <855283.60155.qm@web36213.mail.mud.yahoo.com> Message-ID: <753934.54447.qm@web61223.mail.yahoo.com> Jonathan: Usually (I repeat usually) a section falls after IHC if the tissue has had some processing problems and it was not completely infiltrated, usually also because there was a problem with fixation. Under those circumstances, also usually the section is "microscopically uneven", meaning that it cannot adhere properly and completely to the slide and begins to microscopically peel-off or to separate from the glass until it finally falls-off completely. Even if you did not experience this problem before, perhaps because of any problem with processing, it now is happening. Check your processing protocol and its QA: have there been any changes? New reagents manufacturer? A new operator? Change in some procesing times? Same fixation period? Same source of the specimens? Any of these factors could influence. If you have not changed the IHC protocol at all, the cause has to be in the "pre-IHC" steps. Hope this will help you! Ren? J. Jonathan De La Rosa wrote: HELLO, I'M HAVING A SECTION (BREAST) THAT HAS FALLEN OFF SEVERAL TIMES AFTER IHC RUN. THIS HAS BEEN ATTEMPTED 4 TIMES AND IS THE FIRST TIME IVE EXPERIENCED IT TO THIS DEPT. ANY SUGGESTIONS ? Jonathan N. De La Rosa HT New Braunfels, TX 78130 210-412-5837 JDhisto@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From caron_fournier <@t> yahoo.ca Fri Dec 22 10:35:37 2006 From: caron_fournier <@t> yahoo.ca (caron fournier) Date: Fri Dec 22 10:35:45 2006 Subject: [Histonet] digital image analysis systems Message-ID: <20061222163537.70823.qmail@web35406.mail.mud.yahoo.com> Dear Richard and Chen: As David said there are many out there and no one does everything so the best way is the figure out specifically what you want it to be able to do and then get some demo's as you mentioned. If the company is any good they should be able to show you what their software can do with your specific data. If they can not do this then RUN because you will not get the support you want or need with these kind of complicated software packages. One of the one's that you may want to look at is Image Pro by Media Cybernetics (www.mediacy.com). This has biological as well as industrial applications but can be specially designed to your needs by the use of macros. It is (depending on the application) sometimes not user friendly but none of the high end applications are unless you know about image analysis and computers. This company though does have some very knowledgeable people and does run training courses regularly. But, the most important thing is to see if it works with your application and data and to see if the local rep knows what they are doing or you will be frustrated. They also have a users group forum like this one that you can pick other's brains on. Check it out. Caron Caron Fournier, BSc, R.T. Department of Orthopaedics, Division of Orthopaedic Engineering Research, U.B.C. ------------------------------ Message: 14 Date: Sat, 9 Dec 2006 16:52:48 +1100 From: "David Finkelstein" Subject: [Histonet] RE: digital image analysis systems... To: Message-ID: <000701c71b56$41d676a0$0600a8c0@davidfink> Content-Type: text/plain; charset="us-ascii" Dear Richard and Chen It depends on what type of analysis you need to do. I do not think there is one package that does all. The commercial packages can be very expensive and usually don't live up to expectation. One of the best is free from NIH!! http://rsb.info.nih.gov/ij/ If you let me know your specific need I can be a bit more specific. David Finkelstein (PhD), The Mental Health Research Institute of Victoria, dfinkelstein@mhri.edu.au Date: Fri, 8 Dec 2006 11:56:26 -0500 From: "Chen, Shu-Cheng" Subject: RE: [Histonet] digital image analysis systems... To: Cc: "Breckenridge, Richard A" Message-ID: <886D951F4246D94DAF28C82DC102DDD0024F1B3C@KENMSG20.us.schp.com> Content-Type: text/plain; charset="us-ascii" I am interested in this information too. Please include me in your reply. Thanks. Shu-Cheng -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breckenridge, Richard A Sent: Friday, December 08, 2006 11:12 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] digital image analysis systems... Hi all, Just looking for feedback on image analysis systems that are out there. I plan on demo-ing some and any info is welcome. Thanks! Rick Richard A. Breckenridge, HT(ASCP) Technical Director/ Chief Histology Technician University of Texas- Houston Medical School Histology/ IHC Laboratory Office 713-500-6792 IHC Lab 713-500-5096 Histology Lab 713-500-5363 FAX 713-500-0733 Email - Richard.Breckenridge@uth.tmc.edu ------------------------------ Message: 15 Date: Sat, 9 Dec 2006 12:40:16 -0500 From: "joseph pastore" Subject: [Histonet] Lab Vision Detection System To: Message-ID: <00fb01c71bb9$1713ecf0$7864a8c0@JGPONLINE> Content-Type: text/plain; charset="iso-8859-1" Has anyone had experience using the Lab Vision detection system. Am in the process of acquiering a IHC stainer on a reagent rental basis and its come down to either the Biocare Nemesis or the Lab Vision stainer. (Both are exactly the same. ) I am currently using the Biocare Mach 4 detection system and biocare and Dako antibodies, both of which I'm very happy with. I have had no experience with Lab Vision products and would hate to have to change my protocols to fit their products if they're inferior to Biocares. I'm at a VA hospital and am forced to go with GSA contract vendors. If I get a stainer now it would have to be Lab Vision as they have a GSA contract. I could also wait until Biocare gets a GSA which is what I'm thinking right now. Any comments on this???? ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 37, Issue 11 **************************************** __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From Melissa.Gonzalez <@t> cellgenesys.com Fri Dec 22 13:09:37 2006 From: Melissa.Gonzalez <@t> cellgenesys.com (Melissa Gonzalez) Date: Fri Dec 22 13:09:55 2006 Subject: [Histonet] RE: digital image analysis systems Message-ID: Richard & Chen: I agree completely with Caron's message below. We've been very happy with Image Pro applications, and they have great support. Usually, the best of any kind of product comes with great support. Make a wish list of different things you would like to analyze, pick out some images to use as examples, and see which program best supports these examples. Whatever you are most satisfied with is your answer. Happy Holidays, Melissa Message: 8 Date: Fri, 22 Dec 2006 08:35:37 -0800 (PST) From: caron fournier Subject: RE: [Histonet] digital image analysis systems To: histonet@lists.utsouthwestern.edu Message-ID: <20061222163537.70823.qmail@web35406.mail.mud.yahoo.com> Content-Type: text/plain; charset=ascii Dear Richard and Chen: As David said there are many out there and no one does everything so the best way is the figure out specifically what you want it to be able to do and then get some demo's as you mentioned. If the company is any good they should be able to show you what their software can do with your specific data. If they can not do this then RUN because you will not get the support you want or need with these kind of complicated software packages. One of the one's that you may want to look at is Image Pro by Media Cybernetics (www.mediacy.com). This has biological as well as industrial applications but can be specially designed to your needs by the use of macros. It is (depending on the application) sometimes not user friendly but none of the high end applications are unless you know about image analysis and computers. This company though does have some very knowledgeable people and does run training courses regularly. But, the most important thing is to see if it works with your application and data and to see if the local rep knows what they are doing or you will be frustrated. They also have a users group forum like this one that you can pick other's brains on. Check it out. Caron Caron Fournier, BSc, R.T. Department of Orthopaedics, Division of Orthopaedic Engineering Research, U.B.C. ------------------------------ Message: 14 Date: Sat, 9 Dec 2006 16:52:48 +1100 From: "David Finkelstein" Subject: [Histonet] RE: digital image analysis systems... To: Message-ID: <000701c71b56$41d676a0$0600a8c0@davidfink> Content-Type: text/plain; charset="us-ascii" Dear Richard and Chen It depends on what type of analysis you need to do. I do not think there is one package that does all. The commercial packages can be very expensive and usually don't live up to expectation. One of the best is free from NIH!! http://rsb.info.nih.gov/ij/ If you let me know your specific need I can be a bit more specific. David Finkelstein (PhD), The Mental Health Research Institute of Victoria, dfinkelstein@mhri.edu.au Date: Fri, 8 Dec 2006 11:56:26 -0500 From: "Chen, Shu-Cheng" Subject: RE: [Histonet] digital image analysis systems... To: Cc: "Breckenridge, Richard A" Message-ID: <886D951F4246D94DAF28C82DC102DDD0024F1B3C@KENMSG20.us.schp.com> Content-Type: text/plain; charset="us-ascii" I am interested in this information too. Please include me in your reply. Thanks. Shu-Cheng -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breckenridge, Richard A Sent: Friday, December 08, 2006 11:12 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] digital image analysis systems... Hi all, Just looking for feedback on image analysis systems that are out there. I plan on demo-ing some and any info is welcome. Thanks! Rick Richard A. Breckenridge, HT(ASCP) Technical Director/ Chief Histology Technician University of Texas- Houston Medical School Histology/ IHC Laboratory Office 713-500-6792 IHC Lab 713-500-5096 Histology Lab 713-500-5363 FAX 713-500-0733 Email - Richard.Breckenridge@uth.tmc.edu From opiecurt <@t> yahoo.com Fri Dec 22 13:28:34 2006 From: opiecurt <@t> yahoo.com (curt tague) Date: Fri Dec 22 13:28:42 2006 Subject: [Histonet] looking to barter control blocks Message-ID: <606324.4575.qm@web81613.mail.mud.yahoo.com> i've got a ton of h-pylori and am running a tad bit short of AFB. anyone interested in a hostage exchange? curt __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From mmcgraw <@t> seattlecca.org Fri Dec 22 16:50:08 2006 From: mmcgraw <@t> seattlecca.org (McGraw, Medea J) Date: Fri Dec 22 16:50:21 2006 Subject: [Histonet] GMS Precipitant Message-ID: <095BC97447B56C489B3498DE0B30CDD50F3208@storm.seattlecca.org> Peggy, I had a question concerning the Gordon and Sweets Retic stain. I have been getting great results with the fibers picking up the silver quite nicely; however, there are areas within the tissue that have a granular look to them. It looks like the silver precipitated into the surrounding tissue and sort of can interfere with reading out the slides. I am trying to "clean up" the stain and was wondering if you had any suggestions. I have been blocking the sections prior to staining with 0.1% NFR and also a quick counterstain at the end with NFR and it looks great and mostly clean except for these granules. Do you suggest possibly adding more time/ increasing the % of Sodium Thio or would you suggest possibly trying a new reagent of silver. I suspect that the silver that we are using might not be the best quality. If you have any suggestions it would be much appreciated. Thanks and happy holidays. Medea J. McGraw , BS, HT, HTL (ASCP) SCCA Pathology Department Desk: 206-288-1358 Main Lab: 206-288-1355 Email: mmcgraw@seattlecca.org This electronic message transmission contains information which may be confidential or privileged. The information is intended to be for the use of the individual or entity named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution or use of the contents of this information is prohibited. If you have received this electronic transmission in error, please leave a message via telephone at (206) 288-6266, notify me by electronic reply, and delete this message. Opinions and ideas in this message that do not relate to official business are understood as neither given nor endorsed by the Seattle Cancer Care Alliance. To view our complete Notice of Privacy Practices, visit our web site at www.seattlecca.org. From tahseen <@t> brain.net.pk Fri Dec 22 19:56:39 2006 From: tahseen <@t> brain.net.pk (Tahseen) Date: Fri Dec 22 19:56:40 2006 Subject: [Histonet] fall off sections References: <855283.60155.qm@web36213.mail.mud.yahoo.com> Message-ID: <001c01c70ea2$05c0a2f0$2f1480cb@piii> Dear Jonathan N, Try to use positive charge slides. Hope this will help you! Muhammad Tahseen ----- Original Message ----- From: "Jonathan De La Rosa" To: Sent: Friday, December 22, 2006 5:50 PM Subject: [Histonet] fall off sections > HELLO, > > I'M HAVING A SECTION (BREAST) THAT HAS FALLEN OFF SEVERAL TIMES AFTER IHC > RUN. THIS HAS BEEN ATTEMPTED 4 TIMES AND IS THE FIRST TIME IVE EXPERIENCED > IT TO THIS DEPT. ANY SUGGESTIONS ? > > > Jonathan N. De La Rosa HT > New Braunfels, TX 78130 > 210-412-5837 > JDhisto@yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tissuearray <@t> hotmail.com Sat Dec 23 12:05:57 2006 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Sat Dec 23 12:06:08 2006 Subject: [Histonet] Tissue Micro Array - TMA Message-ID: I have a sponsor for my TMA Instrucitonal website: http://www.arrayworkshop.com. The videos are now FREE for everyone to view. The site is still in construction so some stuff in incomplete. But anyways, I hope all can benifit from the information there. Merry Christmas all... Thom Jensen _________________________________________________________________ Find sales, coupons, and free shipping, all in one place!  MSN Shopping Sales & Deals http://shopping.msn.com/content/shp/?ctid=198,ptnrid=176,ptnrdata=200639 From jcline <@t> wchsys.org Tue Dec 26 10:42:19 2006 From: jcline <@t> wchsys.org (Joyce Cline) Date: Tue Dec 26 10:42:27 2006 Subject: [Histonet] Factor VIII question Message-ID: <000201c7290c$cf3c9970$1d2a14ac@wchsys.org> We are a clinical lab and are cutting rat skins for a research wound study. We would like to do a Factor VIII on these skins. Not being in research do I order the same antibody that I would for human tissue? I would order Factor VIII-R Ag. for a blood vessel study. ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. From shive003 <@t> umn.edu Tue Dec 26 12:09:34 2006 From: shive003 <@t> umn.edu (Jan Shivers) Date: Tue Dec 26 12:09:43 2006 Subject: [Histonet] Factor VIII question References: <000201c7290c$cf3c9970$1d2a14ac@wchsys.org> Message-ID: <001e01c72918$ffd17720$a1065486@auxs.umn.edu> Joyce, I haven't used Factor VIII on rats myself, but I've used it on nearly all other species (dogs, cats, pigs, etc.), and it works just fine. Jan Shivers U of MN Vet Diag Lab ----- Original Message ----- From: "Joyce Cline" To: Sent: Tuesday, December 26, 2006 10:42 AM Subject: [Histonet] Factor VIII question > We are a clinical lab and are cutting rat skins for a research wound > study. We would like to do a Factor VIII on these skins. Not being in > research do I order the same antibody that I would for human tissue? I > would order Factor VIII-R Ag. for a blood vessel study. > > > ***** CONFIDENTIALITY NOTICE ***** > This message contains confidential information and is intended only for > the individual named. If you are not the named addressee you should not > disseminate, distribute or copy this e-mail. Please notify the sender > immediately by e-mail if you have received this e-mail by mistake and > delete this e-mail from your system. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Vickroy.Jim <@t> mhsil.com Tue Dec 26 12:13:48 2006 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Tue Dec 26 12:14:00 2006 Subject: [Histonet] paraffins Message-ID: Anybody sampled paraffins lately to find a better product. Currently we are using Richard Allan Type 1 and Type 9. We are having problems occasionally and are ready to sample something new. Don't want to spend a lot of time sampling different ones wondered if someone has done this lately. Thanks Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From doug <@t> ppspath.com Tue Dec 26 12:23:38 2006 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Dec 26 12:20:58 2006 Subject: [Histonet] paraffins In-Reply-To: Message-ID: Jim, I have just gone through the exact same thing. I have settled on Paraplast Plus for processing and Paraplast Xtra for embedding. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Tuesday, December 26, 2006 1:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] paraffins Anybody sampled paraffins lately to find a better product. Currently we are using Richard Allan Type 1 and Type 9. We are having problems occasionally and are ready to sample something new. Don't want to spend a lot of time sampling different ones wondered if someone has done this lately. Thanks Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From DOOLEEO <@t> shands.ufl.edu Tue Dec 26 12:30:40 2006 From: DOOLEEO <@t> shands.ufl.edu (Elaine Dooley) Date: Tue Dec 26 12:31:13 2006 Subject: [Histonet] MGMT Message-ID: Dear Histonetters, I have been trying to get the antibody MGMT ( O6-Methylguanine-DNA-Methyltransferase) to work on a Ventana Benchmark system. I have one control that appeared to work but I have not found any other tissue that has stained. I feel I need to tweak this antibody some perhaps I am getting false negative reactions. I have been using Lab Vision Neomarkers antibody at 1:50 with mild CC1 epitope retrieval with amplifier. (using i-view dab detection) Any comments may be helpful Elaine Dooley HTL Shands Teaching Hosp. Gainesville FL 352-265-0111 ext 72117 From godsgalnow <@t> aol.com Tue Dec 26 12:53:04 2006 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Tue Dec 26 12:53:23 2006 Subject: [Histonet] paraffins In-Reply-To: <200612261321.73c459168151bf@rly-xl06.mx.aol.com> Message-ID: <8C8F73635A094F3-B80-7F32@FWM-D25.sysops.aol.com> I use paraplast plus for infiltration and embedding--used it for years and love it. Roxanne Soto HT(ASCP)QIHC -----Original Message----- From: doug@ppspath.com To: Vickroy.Jim@mhsil.com; histonet@lists.utsouthwestern.edu Sent: Tue, 26 Dec 2006 1:23 PM Subject: RE: [Histonet] paraffins Jim, I have just gone through the exact same thing. I have settled on Paraplast Plus for processing and Paraplast Xtra for embedding. Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim Sent: Tuesday, December 26, 2006 1:14 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] paraffins Anybody sampled paraffins lately to find a better product. Currently we are using Richard Allan Type 1 and Type 9. We are having problems occasionally and are ready to sample something new. Don't want to spend a lot of time sampling different ones wondered if someone has done this lately. Thanks Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From scoop <@t> mail.nih.gov Tue Dec 26 17:32:27 2006 From: scoop <@t> mail.nih.gov (scoop@mail.nih.gov) Date: Tue Dec 26 17:32:37 2006 Subject: [Histonet] lafora bodies and tau ihc Message-ID: Dear All, Does anyone have any experience/protocols for evaluating for Lafora bodies in mouse brain tissue? Do you have to do electron microscopy and is it definitive for identification of Lafora bodies? Also, are there any tricks to demonstrating tau immunohistochemically in Lafora bodies in mouse or any recommended anti-tau antibodies for that purpose? (I can get the ubiquitin IHC to work ok) Thanks in advance for any help! Sharon From barbwebb <@t> webtv.net Wed Dec 27 07:22:44 2006 From: barbwebb <@t> webtv.net (Barbara Webb) Date: Wed Dec 27 07:22:53 2006 Subject: [Histonet] Lafora Disease/Epilepsy Message-ID: I was unfamiliar with this -so Googled-: maybe someone else would be interested. bw http://moon.ouhsc.edu/kfung/jty1/NeuroHelp/ZNF4IE01.htm From doug <@t> ppspath.com Wed Dec 27 08:09:08 2006 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Wed Dec 27 08:06:32 2006 Subject: [Histonet] Job Opening Message-ID: Professional Pathology Services, PC is one of the regions fastest growing Pathology laboratories. It is located in sunny Columbia South Carolina in heart of Carolina Research Park. Our state of the art histology laboratory has a wall of windows with a scenic view of our own pond. Come join our fun and exciting team of healthcare professionals. We are seeking a full-time histology technician to perform technical duties related to production of histolopathological slides. Researches, troubleshoots, and resolves histology related inquiries and problems within the laboratory and for PPS?s clients. Candidate will perform accessioning, processing, embedding, microtomy and staining of specimens. License/Certification/Education: ASCP certified or eligible w/1-5 years of experience in Histology. This position is for the hours of 10PM-6:30AM. Employer may adjust shift in the future if necessary. Email r?sum? to Doug@ppspath.com www.ppspath.com Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. From Janet.Bonner <@t> FLHOSP.ORG Wed Dec 27 09:23:58 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Wed Dec 27 09:26:05 2006 Subject: [Histonet] fall off sections References: <855283.60155.qm@web36213.mail.mud.yahoo.com> <001c01c70ea2$05c0a2f0$2f1480cb@piii> Message-ID: <5F31F38C96781A4FBE3196EBC22D478004A6EC@fhosxchmb006.ADVENTISTCORP.NET> Use "GOLD" slides - nothing comes off those! (From Fisher or Allegiance) ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Tahseen Sent: Wed 11/22/2006 8:52 PM To: Jonathan De La Rosa; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] fall off sections Dear Jonathan N, Try to use positive charge slides. Hope this will help you! Muhammad Tahseen ----- Original Message ----- From: "Jonathan De La Rosa" To: Sent: Friday, December 22, 2006 5:50 PM Subject: [Histonet] fall off sections > HELLO, > > I'M HAVING A SECTION (BREAST) THAT HAS FALLEN OFF SEVERAL TIMES AFTER IHC > RUN. THIS HAS BEEN ATTEMPTED 4 TIMES AND IS THE FIRST TIME IVE EXPERIENCED > IT TO THIS DEPT. ANY SUGGESTIONS ? > > > Jonathan N. De La Rosa HT > New Braunfels, TX 78130 > 210-412-5837 > JDhisto@yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= From opiecurt <@t> yahoo.com Wed Dec 27 11:59:12 2006 From: opiecurt <@t> yahoo.com (curt tague) Date: Wed Dec 27 11:59:20 2006 Subject: [Histonet] re: those AFB and H. Pylori controls Message-ID: <802035.28703.qm@web81609.mail.mud.yahoo.com> i got many responses so to try and meet everyones needs i'm going to have to stain a bunch of these to make sure they are all positive and then i'll start sending them out. i'll be in touch with those of you who are in need in the next few days, Curt, Pathology Arts, 909.890.2532 __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From tim.morken <@t> thermofisher.com Wed Dec 27 12:07:17 2006 From: tim.morken <@t> thermofisher.com (Morken, Tim) Date: Wed Dec 27 12:07:45 2006 Subject: [Histonet] IHC position open at Lab Vision Corporation Message-ID: Here is an open position at Lab Vision Corp (part of ThermoFisher Scientific). Please send resumes to me at the emial address below. Please note that preference will be given to local candidates (California). ************************************************************************ ************************************** Quality Control Research Associate II Summary Performs quality control tests on antibodies, detection systems, and ancillary products used for immunohistochemistry. Performs IHC and works with Technical Support to test retainers and returned lots of possible nonconforming products. Performs sectioning of paraffin tissue blocks and frozen tissue blocks. May participate in special projects involving development of new products, either reagent or instrumentation. May oversee work of QC Research Associate I. Major Responsibilities Performs routine IHC testing of raw materials and manufactured antibody products according to established quality control procedures/permanent work instructions. Performs product IHC stability tests. Releases product as directed by QC IHC Lab Manager. Performs experiments to assess product or procedural problems under direction of QC IHC Lab Manager and/or Technical Support Representative. Maintains and manufactures positive control slide product inventory. Prepares ancillary reagent/buffer products for IHC lab. Performs sectioning of paraffin tissue blocks and frozen tissue blocks. Assists in maintenance of tissue control stock. May be involved in special projects including new product development, product optimization, and optimization requested by sales rep/customers, either reagent or instrumentation. Prepares reports on assigned assays, processes, or quality control tests. Complies with GMP, QSRs, and ISO regulations. Assists in maintaining laboratory equipment to assure accuracy and confidence in performance, and reports equipment problems to Calibration Representative. Complies with Lab Vision/NeoMarkers' Quality Policies and Quality Procedures. Able to work closely with other departments in reaching company goals. Education and/or certification requirements BA/BS in Chemistry, Biochemistry, Biology, or Bioscience with 3 or more years relevant experience. Able to perform all specific laboratory procedures as requested by the QC IHC Lab Manager. Ability to meet deadlines and ensure successful product release in a timely manner. Must be able to write clear and understandable documentation. Good word processing and spreadsheet skills. Familiar with antibody technology, especially a good understanding of proteins, enzymes, and the structures and functions of antibodies. Able to work in a team environment. Certification as Histotechnologist (HTL) and Qualification for Immunohistochemistry (QIHC) by American Society for Clinical Pathology (ASCP) is desirable. Supervisory Reponsibilities May oversee work of QC Research Associate I. Lab Vision, part of ThermoFisher Scientific, manufactures antibodies and automated immunohistochemistry staining platforms for the clinical and research histology laboratory. Lab Vision is located in Fremont, California in the heart of the San Francisco Bay area biotechnology industry. ************************************************************************ *************************** Tim Morken Product Development Lab Vision - Neomarkers ThermoFisher Scientific 47777 Warm Springs Blvd. Fremont, CA 94539 USA tel: 01-510-991-2840 fax: 01 510-991-2826 tpmorken@labvision.com or Tim.Morken@thermofisher.com web: www.labvision.com www.thermofisher.com From 1dpeterson <@t> meriter.com Wed Dec 27 12:38:28 2006 From: 1dpeterson <@t> meriter.com (Peterson, Dan) Date: Wed Dec 27 12:38:38 2006 Subject: [Histonet] Fading Away Message-ID: <328CBAE62F31C642B422970E879DFADC01A80161@pcwex01> Histonetters, I've read past the discussions about Heme fading on slides but haven't seen anything regarding cover slips. We switched to tape cover slip film about 5-6 years ago. We also switched to mercury-free Heme. Strangely, that seems to be when our Heme began disappearing from the slides. Could it be the tape? The type of Heme (we use Protocol - Harris) Daniel R Peterson HT(ASCP) Histopathology Section Head General Medical Laboratories (608)-267-6557 1dpeterson@meriter.com CONFIDENTIALITY NOTICE: This message (including any attachments) is intended for the sole use of the individual and entity to whom it is addressed. This message may contain information that is confidential and is protected by law. If you are not the intended recipient, you are hereby notified that any disclosure, copying or distribution of this message is strictly prohibited. If you received this message in error, please immediately notify the sender by reply email and then delete the message. Thank you. From rachael_emerson <@t> urmc.rochester.edu Thu Dec 28 09:15:09 2006 From: rachael_emerson <@t> urmc.rochester.edu (Rachael Emerson) Date: Thu Dec 28 09:15:29 2006 Subject: [Histonet] Plastic embedding Message-ID: Hello. I am thinking about doing some plastic embedding. It?s been years since I have worked in that area and I would really appreciate some pointers. What?s the latest kits available that people have found to be successful and efficient? Are there any reference websites that you would recommend? Last time I worked in this area I was using JB4 with mixed results, but that was over 5 years ago. I appreciate your thoughts and suggestions. Thank you Rachael Emerson -- Rachael L. Emerson Center for Pediatric Biomedical Research University of Rochester Medical Center 575 Elmwood Avenue MRBX 1.11301 Rochester, NY 14642 From Jacqueline.Malam <@t> rli.mbht.nhs.uk Thu Dec 28 09:31:42 2006 From: Jacqueline.Malam <@t> rli.mbht.nhs.uk (Malam Jacqueline) Date: Thu Dec 28 09:36:34 2006 Subject: [Histonet] RE: Slide adhesion Message-ID: If you still have trouble, it might be worth looking at the batch number of the problem slides and trying another lot - contact the suppliers and they should be able to oblige. Another thing to consider is that any water used to float out sections must be clean because, if not, any contaminants could attach to the slide and reduce its effectiveness. Good luck Jacqui DISCLAIMER: This e-mail is confidential and privileged. If you are not the intended recipient please accept our apologies; please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Please inform postmaster@rli.mbht.nhs.uk that this message has gone astray before deleting it. Comments or opinions expressed in this email are those of their respective contributors only. The views expressed do not represent the views of the Trust, its management or employees. University Hospitals of Morecambe Bay NHS Trust is not responsible and disclaims any and all liability for the content of comments written within.Thank you for your co-operation. From tmhpath <@t> amigo.net Thu Dec 28 09:44:51 2006 From: tmhpath <@t> amigo.net (Michelle D. Moore) Date: Thu Dec 28 09:43:06 2006 Subject: [Histonet] Malarial smear Message-ID: <000601c72a97$1de3b8c0$ef00a8c0@tmhpath1> Good morning! I have a question I was requested to do a stain for malaria on a blood smear. I have looked through my literature, I have many books, and I cannot find a stain for a smear only for tissue. Could someone direct me to a source or e-mail me a procedure? I would be greatly appreciate it, I am on my last leg. Thank you in advance for your responses. Have a good day and a better tomorrow :) Michelle D. Moore HT (ASCP) TMH 785 Russell St Craig, CO 81625 From Tbarnhart <@t> primecare.org Thu Dec 28 09:49:57 2006 From: Tbarnhart <@t> primecare.org (Barnhart, Tammy) Date: Thu Dec 28 09:50:12 2006 Subject: [Histonet] Malarial smear Message-ID: <5513340D73936645BC3F7B3D3262CDCD79CECF@exchangent> We do a Wright's stain. Tammy Barnhart, BS, HTL(ASCP) Anatomic Pathology Manager St. Alexius Medical Center Bismarck, ND WRIGHT'S/ TZANK'S STAIN PRINCIPLE: Wright/Tzanks stain is a polychromatic stain. The dyes present in the stain produce multiple colors when applied to cells. Wright stain is a mixture of Methylene Blue, Azure B (obtained from oxidation of the Methylene Blue), and Eosin Y dissolved in methanol. The quantities of the dyes used in preparing the Wright stain powder must be carefully controlled to yield a neutral compound dye and optimum staining results. During the staining process, when the buffer solution is added to the stain, ionization occurs, during which time the process of staining the cells takes place. The Eosin ions are negatively charged and stain the basic component of the cells an orange to pink color. The acid structures of the cell are stained varying shades of blue to purple by the positively charged Azure B. Neutrophil granules are probably stained by the Azure compounds. There is a wide variability in the staining characteristics of these stains from one lot to the next, which is most likely due to contaminants present in the dyes. SPECIMEN: Smears prepared on standard high quality glass slides. Allow the smear to thoroughly air dry. CONTROL: Internal patient control is used. REAGENTS: Wright's Stain 1. 500 ml of 100% Methanol is heated to 50C in a water bath. 2. 1.5 gm of Wright's stain powder is added to the methanol and mixed well. 3. The solution is placed in the oven at 37C overnight. 4. Filtered and placed in dark bottle 5. Keep at room temperature. Distilled Water PROCEDURE: 1. Place air-dried smears with the smear side up on a stain rack. 2. Flood slides with Wright stain. Set timer for 2 minute. 3. Buffer the slides with a small volume of distilled water Mix the two reagents on the slide by gently blowing back and forth over the solution. A metallic sheen should form on top of mixture. Set timer for 7 minutes for peripheral smear and 10 minutes for Bone Marrow, FNA and Lymph Node imprint slides. 4. Rinse the slides thoroughly with a stream of distilled water. 5. Wipe off the back of slide and stand on end in a rack to dry. 6. Dip in xylene and coverslip. DISCUSSION: A well-stained smear will show pink to orange red blood cells, dark purple nuclei in lymphocytes and neutrophils, a lighter purple nucleus in monocytes, bright orange granules in eosinophils, dark blue black granules in basophils, and violet to purple platelet granules. The cytoplasm of monocytes is a gray blue with fine reddish granules. The neutrophil has a light pink cytoplasm with lilac granules, and the lymphocyte shows varying shades of blue cytoplasm. Many factors can alter staining. During staining the buffer controls the pH of the stain. If the pH is too acid, those cells or cell parts taking up an acid dye will stain pinker and the acid components that stain with the basic dye show very pale staining. If the stain-buffer mixture is too alkaline, the red blood cells will appear grayish-blue and the white cell nuclei will stain very deeply purple. Therefore to stain all cells well the pH of the buffer is critical. The staining rack must be level to guard against uneven staining of the smear. Insufficient washing of the smears when removing the stain and buffer mixture may cause precipitate on the dried smear. Leaving water on the smear after rinsing or prolonged rinsing causes the stain to fade. REFERENCE: Hematology: Principles and Procedures, Barbara A. Brown, Sixth Edition, 1993, pp. 100 - 102 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Michelle D. Moore Sent: Thursday, December 28, 2006 9:45 AM To: Histonet Subject: [Histonet] Malarial smear Good morning! I have a question I was requested to do a stain for malaria on a blood smear. I have looked through my literature, I have many books, and I cannot find a stain for a smear only for tissue. Could someone direct me to a source or e-mail me a procedure? I would be greatly appreciate it, I am on my last leg. Thank you in advance for your responses. Have a good day and a better tomorrow :) Michelle D. Moore HT (ASCP) TMH 785 Russell St Craig, CO 81625 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Thu Dec 28 10:03:08 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Thu Dec 28 10:03:28 2006 Subject: [Histonet] Malarial smear Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD32037D2DE8@sjhaexc02.sjha.org> Wright's stain will work fine.... -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Michelle D. Moore Sent: Thursday, December 28, 2006 10:45 AM To: Histonet Subject: [Histonet] Malarial smear Good morning! I have a question I was requested to do a stain for malaria on a blood smear. I have looked through my literature, I have many books, and I cannot find a stain for a smear only for tissue. Could someone direct me to a source or e-mail me a procedure? I would be greatly appreciate it, I am on my last leg. Thank you in advance for your responses. Have a good day and a better tomorrow :) Michelle D. Moore HT (ASCP) TMH 785 Russell St Craig, CO 81625 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From JEllin <@t> yumaregional.org Thu Dec 28 10:25:42 2006 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Thu Dec 28 10:26:02 2006 Subject: [Histonet] Wage and Productivity Message-ID: <29BE166A2CF48D459853F8EC57CD37E87A6F05@EXCHANGECLUSTER.yumaregional.local> I was wondering of anyone that is willing to share wage information for the southwest region of the USA. Currently we are trying to build a case, so that we can go to management for increases. The infomormation that we have from ASCP, helps, but they are wanting more. I have gone online and looked at job postings and it seems the starting salary ranges from $23.00 to 26.00. Also is there any articles out there that pertain to how many blocks a tech should cut?? Jesus A. Ellin HT ASCP Yuma Regional Medical Center Histology Systems Technologist Pathology Information Systems 928-336-7444 or 928-336-1144 Fax: 928-336-7319 This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. From rjbuesa <@t> yahoo.com Thu Dec 28 11:03:13 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Dec 28 11:03:19 2006 Subject: [Histonet] Section adhesion to slides Message-ID: <896920.28711.qm@web61220.mail.yahoo.com> For really difficult sections (decals, toe nails, poorly infiltrated breasts and the likke) I used 2 methods (besides the "conventional" + charged slides): 1- coat the slides with a 5% Elmer's glue solution; let the slides dry and use for the sections the next day (or when needed); or 2- add 1 mL of Elmer's glue to a regular 2 litres water bath (to make a 0.05% solution) and use it as your regular water bath (in case you have many difficult cases). The water will have a "milky" appearance, but that is OK Elmer's glue does not interfere with any regular or special stains, nor with IHC procedures. Ren? J. __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From tmhpath <@t> amigo.net Thu Dec 28 11:06:28 2006 From: tmhpath <@t> amigo.net (Michelle D. Moore) Date: Thu Dec 28 11:04:41 2006 Subject: [Histonet] Thank you for malaria info Message-ID: <001c01c72aa2$8486e510$ef00a8c0@tmhpath1> Thank you all so much for your response to my malaria question. This is such a great place for information. I appreciate all you time very much. Have a grateful day. Michelle D. Moore TMH 785 Russell St Craig, CO 81625 From rjbuesa <@t> yahoo.com Thu Dec 28 11:07:23 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Dec 28 11:07:28 2006 Subject: [Histonet] Malarial smear In-Reply-To: <000601c72a97$1de3b8c0$ef00a8c0@tmhpath1> Message-ID: <20061228170723.32549.qmail@web61224.mail.yahoo.com> Any of the Romanowski's types stains (Giemsa, May-Gr?nwald or Wright) stains will give you good results. Stain the smear as if it were just for blood. Ren? J. "Michelle D. Moore" wrote: Good morning! I have a question I was requested to do a stain for malaria on a blood smear. I have looked through my literature, I have many books, and I cannot find a stain for a smear only for tissue. Could someone direct me to a source or e-mail me a procedure? I would be greatly appreciate it, I am on my last leg. Thank you in advance for your responses. Have a good day and a better tomorrow :) Michelle D. Moore HT (ASCP) TMH 785 Russell St Craig, CO 81625 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From rjbuesa <@t> yahoo.com Thu Dec 28 11:11:14 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Dec 28 11:11:21 2006 Subject: [Histonet] Wage and Productivity In-Reply-To: <29BE166A2CF48D459853F8EC57CD37E87A6F05@EXCHANGECLUSTER.yumaregional.local> Message-ID: <570885.99783.qm@web61222.mail.yahoo.com> Jes?s: Under separate cover I am sending you an article I published on productivity. The is a salary survey in Lab. Medicine from August 2006 (vol37, no.8;pp465-9). Ren? J. Jesus Ellin wrote: I was wondering of anyone that is willing to share wage information for the southwest region of the USA. Currently we are trying to build a case, so that we can go to management for increases. The infomormation that we have from ASCP, helps, but they are wanting more. I have gone online and looked at job postings and it seems the starting salary ranges from $23.00 to 26.00. Also is there any articles out there that pertain to how many blocks a tech should cut?? Jesus A. Ellin HT ASCP Yuma Regional Medical Center Histology Systems Technologist Pathology Information Systems 928-336-7444 or 928-336-1144 Fax: 928-336-7319 This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From Amanda.Garcia <@t> TriadHospitals.com Thu Dec 28 12:02:25 2006 From: Amanda.Garcia <@t> TriadHospitals.com (Garcia, Amanda) Date: Thu Dec 28 12:04:12 2006 Subject: [Histonet] unsubscribe Message-ID: <8B63039C9DF4554C8FDBF31235F0E148027F2F87@CPRTEVS02.triadhospitals.net> > Amanda (Amy) Garcia > > Histology/Pathology > College Station Medical Center > (979) 680-5372 office > (979) 696-5446 fax > *Mailto:amanda.garcia@triadhospitals.com > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the e-mail or any > of its attachments, please be advised that you have received this > e-mail in error and that any use, dissemination, distribution, > forwarding, printing, or copying of this e-mail or any attached files > is strictly prohibited. If you have received this e-mail in error, > please immediately purge it and all attachments and notify the sender > by reply e-mail or contact the sender at the number listed. > > > From godsgalnow <@t> aol.com Thu Dec 28 13:04:46 2006 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Thu Dec 28 13:05:00 2006 Subject: [Histonet] Wage and Productivity In-Reply-To: <29BE166A2CF48D459853F8EC57CD37E87A6F05@EXCHANGECLUSTER.yumaregional.local> References: <29BE166A2CF48D459853F8EC57CD37E87A6F05@EXCHANGECLUSTER.yumaregional.local> Message-ID: <8C8F8CA2D0C6CEE-B70-361D@FWM-D35.sysops.aol.com> I use 1 per minute for my standard. Here is zFlorida, salary varies from region to region and whether or not you work for a hospital or a private lab. Traditionally, hospitals pay less (about 2-3 dollars an hour less than private labs. I know at my lab, I start technicians out at $23 - $24/hour and technologist $25- $26/hour. Roxanne Soto HT(ASCOP)QIHC Lab Manager Physicians RightPath Tampa, Florida -----Original Message----- From: JEllin@yumaregional.org To: histonet@lists.utsouthwestern.edu Sent: Thu, 28 Dec 2006 11:25 AM Subject: [Histonet] Wage and Productivity I was wondering of anyone that is willing to share wage information for the southwest region of the USA. Currently we are trying to build a case, so that we can go to management for increases. The infomormation that we have from ASCP, helps, but they are wanting more. I have gone online and looked at job postings and it seems the starting salary ranges from $23.00 to 26.00. Also is there any articles out there that pertain to how many blocks a tech should cut?? Jesus A. Ellin HT ASCP Yuma Regional Medical Center Histology Systems Technologist Pathology Information Systems 928-336-7444 or 928-336-1144 Fax: 928-336-7319 This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From AWempren <@t> skcc.org Thu Dec 28 14:17:28 2006 From: AWempren <@t> skcc.org (Alexina Wempren) Date: Thu Dec 28 14:17:37 2006 Subject: [Histonet] inflammatory cells? Message-ID: Does anyone know of what antibodies (for mouse, rat, or human) on any and all inflammatory cell to use? Any advice would be greatly appreciated. Thanks for listening:-).............alexina Alexina Wempren Sidney Kimmel Cancer Center La Jolla, CA awempren@skcc.org From robert.kan <@t> us.army.mil Thu Dec 28 14:23:16 2006 From: robert.kan <@t> us.army.mil (Kan, Robert K Dr USAMRICD) Date: Thu Dec 28 14:23:49 2006 Subject: [Histonet] Proccess monkey brains in paraffin Message-ID: I am interested in processing 3mm thick monkey brain slices in paraffin. What is the ideal processing time in each alochol and paraffin? Thank you, Robert From tbraud <@t> holyredeemer.com Thu Dec 28 15:29:55 2006 From: tbraud <@t> holyredeemer.com (Terri Braud) Date: Thu Dec 28 15:31:45 2006 Subject: [Histonet] Wage and Productivity In-Reply-To: <8C8F8CA2D0C6CEE-B70-361D@FWM-D35.sysops.aol.com> Message-ID: There was a very interesting summation of block workloads provided on the Histonet back in '99. I thought it was an excellent reference. Not only did it give a general figure, but a breakdown of differing expectations, based on what was being cut. Here is the link to that archived article: http://www.histosearch.com/histonet/Apr99A/HistoTechPerformanceStand.html For my labs, I generally use 30 per hour, for up to 2 slides per block from mixed tissues, large and small. I hope this helps. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supv. Laboratory, Holy Redeemer Hospital 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3689 -----Original Message----- From: JEllin@yumaregional.org To: histonet@lists.utsouthwestern.edu Sent: Thu, 28 Dec 2006 11:25 AM Subject: [Histonet] Wage and Productivity I was wondering of anyone that is willing to share wage information for the southwest region of the USA. Currently we are trying to build a case, so that we can go to management for increases. The infomormation that we have from ASCP, helps, but they are wanting more. I have gone online and looked at job postings and it seems the starting salary ranges from $23.00 to 26.00. Also is there any articles out there that pertain to how many blocks a tech should cut?? Jesus A. Ellin HT ASCP Yuma Regional Medical Center Histology Systems Technologist Pathology Information Systems 928-336-7444 or 928-336-1144 Fax: 928-336-7319 This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it is addressed. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail at the electronic address shown. Thank you for your cooperation. From AGrobe2555 <@t> aol.com Thu Dec 28 16:24:09 2006 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Thu Dec 28 16:24:23 2006 Subject: [Histonet] Inflammatory Cells Message-ID: I'm afraid you may need to be a bit more specific. Which type of cells are you looking for in which species? From ernestinemiddleton <@t> yahoo.ca Thu Dec 28 20:26:09 2006 From: ernestinemiddleton <@t> yahoo.ca (Ernestine Middleton) Date: Thu Dec 28 20:26:23 2006 Subject: [Histonet] Job Opening Message-ID: <571339.14301.qm@web51507.mail.yahoo.com> Hi; Montefiore Med. Center, Bronx, N. Y. is seeking a histology technician. Hours will be early morning or later evening. This is urgent. You may call, email or fax your resume. Ernestine Middleton, Manager Montefiore Medical Center Bronx, New York 718-920-4157 718-547-1920 Fax emiddlet@montefiore.org __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From Susan.Walzer <@t> HCAHealthcare.com Fri Dec 29 05:32:43 2006 From: Susan.Walzer <@t> HCAHealthcare.com (Walzer Susan) Date: Fri Dec 29 05:32:52 2006 Subject: [Histonet] Dako Transthyretin Antibody In-Reply-To: Message-ID: <471953BC63077941B82C26A4338272B42F04CB@ORLEV03.hca.corpad.net> -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Dana Settembre Sent: Friday, December 15, 2006 1:04 PM To: Histonet; Kelly Turner Subject: Re: [Histonet] Dako Transthyretin Antibody Hello Kelly, I use Dako's Prealbumin on FFPE human tissue with a choriod plexus as a positive control. I use no pretreatment and I use it at 1:8000 with nice results. Good Luck. Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> Kelly Turner 12/14/06 7:34 PM >>> Does anyone happen to be using Dako's polyclonal rabbit anti-human prealbumin (transthyretin) antibody for IHC and willing to share their titer and pretreatment protocol? Our current vendor no longer offers this antibody and Dako's data sheet does not contain much information for its use in IHC applications. Thanks in advance! Kelly Turner, BS, HTL(ASCP), QIHC PhenoPath Laboratories Seattle, WA ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Fri Dec 29 07:30:14 2006 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Dec 29 07:30:19 2006 Subject: [Histonet] Wage and Productivity Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F437@lmhsmail.lmhealth.org> I looked at blocks per hour earlier this year. I think it's more important to look at how things work in your own individual lab than to look at a recommended number that came from ideal or at least unknown cutting conditions. We have 3 cutters of varying experience levels and 3 pathologists with markedly different grossing styles with regard to the samples they submit (trying to be nice there :). It was more important to me to know/document how long the entire process took on average than it was to know how many blocks each person cut. So here's what I did..... Over the period of a few days, every cutter documented the time they started cutting and the time they finished. When they finished, they also documented the number of blocks they cut. Keep in mind that this was the time it took to complete the process. The time included trimming the blocks, phone calls, labeling slides, extras for specials, putting slides in the staining rack and placing them on the stainer, etc., everything that person did while cutting. It was based on our routine case mix, everything from small bx with multiple levels to large specimens... easy blocks to difficult blocks..... Here are the final averages: 2.78 min per block or 21.6 blocks per hour 2.82 min pre block or 21.3 blocks pre hour 3.25 min per block or 18.5 blocks per hour So now I know the average time it should to cut one block is 2.95 minutes and that the process of cutting 100 blocks should take us roughly 2 hours 45 minutes with 2 people cutting. I also did the same for embedding. The time included embedding and trimming the blocks. Here are those averages: 0.96 min per block or 62.5 blocks per hour 1.11 min per block or 54.1 blocks per hour 1.23 min per block or 48.8 blocks per hour The embedding time doesn't add much time to the overall process of getting the slides cut since the cutting starts within 10 to 15 minutes of the start of embedding. So that's what I did. It gives me a much more useful look at that portion of our day and still provides a benchmark based on our workload with our pathologists, and our equipment. I'd be interested to see others.... Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jesus Ellin Sent: Thursday, December 28, 2006 11:26 AM To: Histonet Subject: [Histonet] Wage and Productivity I was wondering of anyone that is willing to share wage information for the southwest region of the USA. Currently we are trying to build a case, so that we can go to management for increases. The infomormation that we have from ASCP, helps, but they are wanting more. I have gone online and looked at job postings and it seems the starting salary ranges from $23.00 to 26.00. Also is there any articles out there that pertain to how many blocks a tech should cut?? Jesus A. Ellin HT ASCP Yuma Regional Medical Center Histology Systems Technologist Pathology Information Systems 928-336-7444 or 928-336-1144 Fax: 928-336-7319 This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TMcNemar <@t> lmhealth.org Fri Dec 29 07:57:57 2006 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Dec 29 07:58:02 2006 Subject: [Histonet] Wage and Productivity Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F438@lmhsmail.lmhealth.org> Cut one block per minute? Can you tell us exactly what is done in that minute? Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of godsgalnow@aol.com Sent: Thursday, December 28, 2006 2:05 PM To: JEllin@yumaregional.org Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Wage and Productivity I use 1 per minute for my standard. Here is zFlorida, salary varies from region to region and whether or not you work for a hospital or a private lab. Traditionally, hospitals pay less (about 2-3 dollars an hour less than private labs. I know at my lab, I start technicians out at $23 - $24/hour and technologist $25- $26/hour. Roxanne Soto HT(ASCOP)QIHC Lab Manager Physicians RightPath Tampa, Florida -----Original Message----- From: JEllin@yumaregional.org To: histonet@lists.utsouthwestern.edu Sent: Thu, 28 Dec 2006 11:25 AM Subject: [Histonet] Wage and Productivity I was wondering of anyone that is willing to share wage information for the southwest region of the USA. Currently we are trying to build a case, so that we can go to management for increases. The infomormation that we have from ASCP, helps, but they are wanting more. I have gone online and looked at job postings and it seems the starting salary ranges from $23.00 to 26.00. Also is there any articles out there that pertain to how many blocks a tech should cut?? Jesus A. Ellin HT ASCP Yuma Regional Medical Center Histology Systems Technologist Pathology Information Systems 928-336-7444 or 928-336-1144 Fax: 928-336-7319 This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From godsgalnow <@t> aol.com Fri Dec 29 08:20:52 2006 From: godsgalnow <@t> aol.com (godsgalnow@aol.com) Date: Fri Dec 29 08:21:05 2006 Subject: [Histonet] Wage and Productivity In-Reply-To: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F438@lmhsmail.lmhealth.org> References: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F438@lmhsmail.lmhealth.org> Message-ID: <8C8F96BAE2723F6-A30-1C3E@FWM-D34.sysops.aol.com> We cut only biopsies here...two levels per slide. We cut one H&E slide and one extra for IHC. During that minute we cut the block and put the sections on the two slides. We have a tech here that can cut 50 blocks and hour producing 2 slides per block. I have another tech that cuts 72 blocks per hour producing 2 slides per block. On the down side, I have a tech that only cuts 24 blocks per hour producing 2 slides per block. Important facts to realize......all of our slides are pre-written. We have good microtomes. We have excellent processing and infiltration. We cut only biopsies. We have excellent techs that have lots of experience. This does not include staining, drying, etc. To come up with my standard I timed all technicians doing all the functions (embedding, cutting, etc). I picked random times (they didn't even know) and timed them doing these functions. I took the average and made this the standard to meet. I used the fastest time as the exceeds standards. I took the slowest time and reduced it by 15% and made this does not meet standards. It really isn't fair to use another facilities standards and make them your own. Everyone has different tissues, processing times, equipment etc. So it is best to do something like this in your facility to create your own standards. Hope this answers your question......... Roxanne -----Original Message----- From: TMcNemar@lmhealth.org To: godsgalnow@aol.com; JEllin@yumaregional.org Cc: histonet@lists.utsouthwestern.edu Sent: Fri, 29 Dec 2006 8:57 AM Subject: RE: [Histonet] Wage and Productivity Cut one block per minute? Can you tell us exactly what is done in that minute? Thanks. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of godsgalnow@aol.com Sent: Thursday, December 28, 2006 2:05 PM To: JEllin@yumaregional.org Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Wage and Productivity I use 1 per minute for my standard. Here is zFlorida, salary varies from region to region and whether or not you work for a hospital or a private lab. Traditionally, hospitals pay less (about 2-3 dollars an hour less than private labs. I know at my lab, I start technicians out at $23 - $24/hour and technologist $25- $26/hour. Roxanne Soto HT(ASCOP)QIHC Lab Manager Physicians RightPath Tampa, Florida -----Original Message----- From: JEllin@yumaregional.org To: histonet@lists.utsouthwestern.edu Sent: Thu, 28 Dec 2006 11:25 AM Subject: [Histonet] Wage and Productivity I was wondering of anyone that is willing to share wage information for the southwest region of the USA. Currently we are trying to build a case, so that we can go to management for increases. The infomormation that we have from ASCP, helps, but they are wanting more. I have gone online and looked at job postings and it seems the starting salary ranges from $23.00 to 26.00. Also is there any articles out there that pertain to how many blocks a tech should cut?? Jesus A. Ellin HT ASCP Yuma Regional Medical Center Histology Systems Technologist Pathology Information Systems 928-336-7444 or 928-336-1144 Fax: 928-336-7319 This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ________________________________________________________________________ Check out the new AOL. Most comprehensive set of free safety and security tools, free access to millions of high-quality videos from across the web, free AOL Mail and more. From tkngflght <@t> yahoo.com Fri Dec 29 09:18:13 2006 From: tkngflght <@t> yahoo.com (Cheryl) Date: Fri Dec 29 09:18:18 2006 Subject: [Histonet] Wage and Productivity In-Reply-To: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F438@lmhsmail.lmhealth.org> Message-ID: <20061229151813.17712.qmail@web50914.mail.yahoo.com> Hi Tom- We staff traveling histologist (up to 50 on the road at a time) and I travel as a temp. What the others say about taking samples and averaging the times of your techs is germaine. I travel to different labs (over 40 labs in 25 years and counting) and I can cut anywhere between 25 and 75 blocks per hour depending on processing, tissue, protocols, instrumentation and automation. The goal on collecting these averages be an acceptable range of time to accommodate all tissue types/cutting protocols and tech skills. The idea behind averaging is to get a benchmark (metric) on how to manage workloads, not to push techs to cut more and faster. That's when you start to get mistakes and repetitive motion injuries....not to mention unhappy campers who will start to look for new employment. If you have a chance to go to other labs, often you can learn little tricks to pick up efficiency without compromising the quality or pushing past your tech's abilities. Each lab is different--not everything works in every lab. It can be fun to try each different idea to see what does work for your lab. Hope this helps! Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one Tech at a time. 281.883.7704 c 281.852.9457 o admin@fullstaff.org Sign up for the FREE monthly newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. From cmiller <@t> physlab.com Fri Dec 29 09:28:55 2006 From: cmiller <@t> physlab.com (Cheri Miller) Date: Fri Dec 29 09:29:02 2006 Subject: [Histonet] Wage and Productivity In-Reply-To: <20061229151813.17712.qmail@web50914.mail.yahoo.com> Message-ID: <000001c72b5e$0d6743a0$db01a8c0@plab.local> Very well said Cheryl, I agree wholeheartedly Cheri MIller HT ASCP Histology Supervisor, Phys Laboratory. Omaha Ne -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: Friday, December 29, 2006 9:18 AM To: TMcNemar@lmhealth.org Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Wage and Productivity Hi Tom- We staff traveling histologist (up to 50 on the road at a time) and I travel as a temp. What the others say about taking samples and averaging the times of your techs is germaine. I travel to different labs (over 40 labs in 25 years and counting) and I can cut anywhere between 25 and 75 blocks per hour depending on processing, tissue, protocols, instrumentation and automation. The goal on collecting these averages be an acceptable range of time to accommodate all tissue types/cutting protocols and tech skills. The idea behind averaging is to get a benchmark (metric) on how to manage workloads, not to push techs to cut more and faster. That's when you start to get mistakes and repetitive motion injuries....not to mention unhappy campers who will start to look for new employment. If you have a chance to go to other labs, often you can learn little tricks to pick up efficiency without compromising the quality or pushing past your tech's abilities. Each lab is different--not everything works in every lab. It can be fun to try each different idea to see what does work for your lab. Hope this helps! Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one Tech at a time. 281.883.7704 c 281.852.9457 o admin@fullstaff.org Sign up for the FREE monthly newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kathy.Rettman <@t> us.crl.com Fri Dec 29 09:47:36 2006 From: Kathy.Rettman <@t> us.crl.com (Rettman, Kathy A.) Date: Fri Dec 29 09:47:41 2006 Subject: [Histonet] hisotech needed Message-ID: <79DC2FFD71913342BB04ED8BBC591F24EE6005@wor-crl-exch1.na01.crl.com> Hi all, We have two histotech positions open in our labs. My lab is located a little north of Cincinnati and our smaller lab is in Spencerville, OH, an hour west of Columbus. Please send, fax, or call me if you are interested or know someone who may be interested. Thanks and Happy New Year! Kathy A. Rettman, HT (ASCP) Laboratory Manager Charles River Laboratories, Preclinical Services 6217 Centre Park Drive West Chester, Ohio 45069 513-779-9600 x228 Fax: 513-779-9603 e-mail: kathy.rettman@us.crl.com From TMcNemar <@t> lmhealth.org Fri Dec 29 09:48:05 2006 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Fri Dec 29 09:48:12 2006 Subject: [BULK] RE: [Histonet] Wage and Productivity Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F439@lmhsmail.lmhealth.org> Cheryl, I don't really understand your response but I totally agree more with what it says. My point in my email was much the same as yours.... It is much more important to develope your own benchmark than to use some national number. It has to be relevant to your situation. I don't use it to push anybody and I don't use it to make comparisons. I did it for workload management. Our workload continues to rise every year and with a big new surgery suite set to open next year, I expect it to go higher. For me, the number is a managemnet, justification, and bargaining tool. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: Cheryl [mailto:tkngflght@yahoo.com] Sent: Friday, December 29, 2006 10:18 AM To: Tom McNemar Cc: histonet@lists.utsouthwestern.edu Subject: [BULK] RE: [Histonet] Wage and Productivity Importance: Low Hi Tom- We staff traveling histologist (up to 50 on the road at a time) and I travel as a temp. What the others say about taking samples and averaging the times of your techs is germaine. I travel to different labs (over 40 labs in 25 years and counting) and I can cut anywhere between 25 and 75 blocks per hour depending on processing, tissue, protocols, instrumentation and automation. The goal on collecting these averages be an acceptable range of time to accommodate all tissue types/cutting protocols and tech skills. The idea behind averaging is to get a benchmark (metric) on how to manage workloads, not to push techs to cut more and faster. That's when you start to get mistakes and repetitive motion injuries....not to mention unhappy campers who will start to look for new employment. If you have a chance to go to other labs, often you can learn little tricks to pick up efficiency without compromising the quality or pushing past your tech's abilities. Each lab is different--not everything works in every lab. It can be fun to try each different idea to see what does work for your lab. Hope this helps! Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one Tech at a time. 281.883.7704 c 281.852.9457 o admin@fullstaff.org Sign up for the FREE monthly newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. From tomgalati <@t> hsrl.org Fri Dec 29 12:52:59 2006 From: tomgalati <@t> hsrl.org (Tom Galati) Date: Fri Dec 29 09:53:32 2006 Subject: [Histonet] Substance P-like staining Message-ID: <018301c72b7a$8f823c70$0a00a8c0@GALATILAPTOP> Dear Netters, Does anyone out there routinely stain rat nervous tissue for Substance P? We are a GLP histopath lab looking to find a high quality reference lab to perform some GLP IHC. Please e-mail me if you have such experience with this particular stain and would be interested in discussing the study further. Thank you, Tom Galati Laboratory Director HSRL, Inc. 5930 Main Street Mount Jackson, VA 22842 540.477.4440 Fax: 540.477.4448 tomgalati@hsrl.org www.hsrl.org From tomgalati <@t> hsrl.org Fri Dec 29 12:57:53 2006 From: tomgalati <@t> hsrl.org (Tom Galati) Date: Fri Dec 29 09:58:25 2006 Subject: [Histonet] Spurr's processing Message-ID: <019501c72b7b$3ecfb540$0a00a8c0@GALATILAPTOP> Good Morning Netters, I would like to hear if other folks out there are using Spurrs for plastic embedding of medical devices and/or undecalcified bones. We use GMA, MMA and Technovit currently, but are doing some method development using Spurrs. Would someone be willing to share their processing protocol for Spurrs with me? Your time is greatly appreciated. Sincerely, Tom Galati Laboratory Director HSRL, Inc. 5930 Main Street Mount Jackson, VA 22842 540.477.4440 Fax: 540.477.4448 tomgalati@hsrl.org www.hsrl.org From jmahoney <@t> alegent.org Fri Dec 29 10:18:07 2006 From: jmahoney <@t> alegent.org (Janice Mahoney) Date: Fri Dec 29 10:18:28 2006 Subject: [Histonet] Wage and Productivity In-Reply-To: <20061229151813.17712.qmail@web50914.mail.yahoo.com> References: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F438@lmhsmail.lmhealth.org> <20061229151813.17712.qmail@web50914.mail.yahoo.com> Message-ID: <4594EB5F0200003C0000136B@gwia.alegent.org> I have to add my two LEAN cents worth. It is quite simple to learn your "throughput time" when you have a LEAN lab. When you have all the unnecessary waste removed from you processes you can calculate the number of slides your lab can produce in any given time frame with any number of techs. So, if you are down two techs for a day, you will know what your slide output will be. If you know what your volume of blocks is for any given day, you will be able to calculate when the cutting should be complete. LEAN provides the perfect staffing, productivity and TAT tools. I admit, I am biased. To learn more about how you can LEAN you lab there are many web sites and Histology workshops. Goggle LEAN and Laboratory and Histology. Have fun and Happy New Year. Jan, Omaha NE >>> Cheryl 12/29/2006 9:18 AM >>> Hi Tom- We staff traveling histologist (up to 50 on the road at a time) and I travel as a temp. What the others say about taking samples and averaging the times of your techs is germaine. I travel to different labs (over 40 labs in 25 years and counting) and I can cut anywhere between 25 and 75 blocks per hour depending on processing, tissue, protocols, instrumentation and automation. The goal on collecting these averages be an acceptable range of time to accommodate all tissue types/cutting protocols and tech skills. The idea behind averaging is to get a benchmark (metric) on how to manage workloads, not to push techs to cut more and faster. That's when you start to get mistakes and repetitive motion injuries....not to mention unhappy campers who will start to look for new employment. If you have a chance to go to other labs, often you can learn little tricks to pick up efficiency without compromising the quality or pushing past your tech's abilities. Each lab is different--not everything works in every lab. It can be fun to try each different idea to see what does work for your lab. Hope this helps! Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one Tech at a time. 281.883.7704 c 281.852.9457 o admin@fullstaff.org Sign up for the FREE monthly newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From carl.hobbs <@t> kcl.ac.uk Fri Dec 29 13:11:40 2006 From: carl.hobbs <@t> kcl.ac.uk (Carl Hobbs) Date: Fri Dec 29 13:12:19 2006 Subject: [Histonet] Re: FactorVIII Q Message-ID: <000501c72b7d$2c1ad1d0$0201a8c0@carlba65530bda> I assume you mean Von Villibrand factor? If so, I get good results on pwax sections of rat, using Chemicon's AB7356, after HIER. Please check here http://www.immunoportal.com/index.php and hit the image gallery link on the left...then type in "von" into the search box and hit "enter" on your keyboard. Hope this helps. Best wishes carl ----- Original Message ----- From: To: Sent: Tuesday, December 26, 2006 6:04 PM Subject: Histonet Digest, Vol 37, Issue 28 > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Factor VIII question (Joyce Cline) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 26 Dec 2006 11:42:19 -0500 > From: "Joyce Cline" > Subject: [Histonet] Factor VIII question > To: > Message-ID: <000201c7290c$cf3c9970$1d2a14ac@wchsys.org> > Content-Type: text/plain;charset="us-ascii" > > We are a clinical lab and are cutting rat skins for a research wound > study. We would like to do a Factor VIII on these skins. Not being in > research do I order the same antibody that I would for human tissue? I > would order Factor VIII-R Ag. for a blood vessel study. > > > ***** CONFIDENTIALITY NOTICE ***** > This message contains confidential information and is intended only for > the individual named. If you are not the named addressee you should not > disseminate, distribute or copy this e-mail. Please notify the sender > immediately by e-mail if you have received this e-mail by mistake and > delete this e-mail from your system. > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 37, Issue 28 > **************************************** From kemlo <@t> f2s.com Sat Dec 30 02:46:22 2006 From: kemlo <@t> f2s.com (kemlo) Date: Sat Dec 30 02:46:34 2006 Subject: [Histonet] Wage and Productivity In-Reply-To: <20061229151813.17712.qmail@web50914.mail.yahoo.com> Message-ID: <001601c72bee$fc658170$0302a8c0@KEMLOS> Very interesting debate that really identifies differences between America and the UK; you Americans appear to be interested in productivity and LEAN management whilst we Brits are more interested in moving to the 'left' and taking on roles from our clinical colleagues. I suppose it depends on your perspective and who pays your wage. A4C has rewarded Brits rather well in the UK and I guess Associate Practitioners and MLAs will now become more, um...... Cost effective???? Mr Kemlo Rogerson MSc DMS MIBiol CBiol FIBMS Pathology Services Manager Weston General Hospital North Somerset -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Cheryl Sent: 29 December 2006 15:18 To: TMcNemar@lmhealth.org Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Wage and Productivity Hi Tom- We staff traveling histologist (up to 50 on the road at a time) and I travel as a temp. What the others say about taking samples and averaging the times of your techs is germaine. I travel to different labs (over 40 labs in 25 years and counting) and I can cut anywhere between 25 and 75 blocks per hour depending on processing, tissue, protocols, instrumentation and automation. The goal on collecting these averages be an acceptable range of time to accommodate all tissue types/cutting protocols and tech skills. The idea behind averaging is to get a benchmark (metric) on how to manage workloads, not to push techs to cut more and faster. That's when you start to get mistakes and repetitive motion injuries....not to mention unhappy campers who will start to look for new employment. If you have a chance to go to other labs, often you can learn little tricks to pick up efficiency without compromising the quality or pushing past your tech's abilities. Each lab is different--not everything works in every lab. It can be fun to try each different idea to see what does work for your lab. Hope this helps! Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one Tech at a time. 281.883.7704 c 281.852.9457 o admin@fullstaff.org Sign up for the FREE monthly newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From s.tripathy <@t> student.qut.edu.au Mon Dec 11 16:59:37 2006 From: s.tripathy <@t> student.qut.edu.au (Srikant Tripathy) Date: Tue Jan 2 08:16:32 2007 Subject: [Histonet] Cryosectioning of retina/choroid/sclera? In-Reply-To: <6.0.0.22.1.20061211093958.01b6fff0@gemini.msu.montana.edu> Message-ID: <001001c71d77$f60fc1d0$baacb583@ihbi.qut.edu.au> Hi, Thanks for your reply. No, I do not want to fix the layers in fixative like formalin or Paraformaldehyde as these are diluted solutions and may dissolve the soluble ion I am trying to estimate. Secondly to fix these tissues I need to take a thin section and this may disturb the ion concentration results. Is it possible for cryosectioning the fragile retina after snap freezing of fresh tissue, with out cryoprotectant? Many Thanks again, Srikant. -----Original Message----- From: Gayle Callis [mailto:gcallis@montana.edu] Sent: Tuesday, 12 December 2006 2:42 AM To: Srikant Tripathy Subject: Re: [Histonet] Cryosectioning of retina/choroid/sclera? Are you fixing the layers first with either formalin or paraformaldehyde? That is when you need cryoprotection, after total fixtaion in 30% sucrose overnight or longer at 4C. Then you can snap freeze. If you are freezing the tissues in fresh state, you do NOT need cryoprotection. These fixatives are often buffered with sodium and potassium phosphate salts, so beware, you may NOT want to add more elements to the system. At 06:36 PM 12/5/2006, you wrote: >Hi, > >I am Srikant a PhD student in Queensland University of >Technology,Brisbane,Australia.In a part of my study I am trying to estimate >the element concentration (Na,K,Cl) at various layers of eye >(retina,choroid,sclera) by EDX analysis and confocal microscope. > >I am a Pharmacist and don't have very good knowledge of histological >techniques. > > > > I am using cryosectioning of eye layers and for a fragile layer like retina >I need to cryoprotect before sectioning. I am using snap freezing >technology. > > > >My doubts are: > >1. As I can't use water soluble cryoprotectant as this will leach all >soluble ion. I wonder how people can keep retina soaked in Ames's solution >and gets fluorescence for chloride ions using dyes like MEQ. >2. Can any one please guide me how I will proceed for cryoprotecting >and sectioning the retina/choroid/sclera with out adverse effects on >elemental concentration? >3. Can I use PEG as a coating material prior to cryosectioning for >elemental analysis before EDX and confocal microscopy? > > > >Thanks. > >Srikant > > > > > >Srikant Tripathy | PhD Student | Institute of Health and Biomedical >Innovation > >Queensland University of Technology | Corner of Musk Avenue and Blamey >Street, Kelvin Grove QLD 4059 Australia > >t: +61 (0) 7 3138 6157 | f: +61 (0) 7 3138 6030 | m: 0423586276 | e: >s.tripathy@qut.edu.au | w: > www.ihbi.qut.edu.au > > > >CRICOS No. 00213J > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From bjbenn01 <@t> louisville.edu Tue Dec 19 14:07:52 2006 From: bjbenn01 <@t> louisville.edu (Barbara J Bishop) Date: Tue Jan 2 16:58:41 2007 Subject: [Histonet] Mitochondria Staining Message-ID: <4588004B.C9D1.004E.0@gwise.louisville.edu> I am interested in finding out if any of you have a good technique for staining mitochondria (preferably the membrane) in formalin-fixed paraffin embedded tissue. We would like a stain that shows great contrast so we can take pictures and then threshold the color of the mitochondria to get a measurement of the amount of mitochondria in the tissue sample. Thank you for all your help. Barbara Bishop, BS, HT(ASCP) Research Technologist II University of Louisville Division of Cardiology Baxter II Rm 434 Lousiville, KY bjbenn01@gwise.louisville.edu