[Histonet] Deparaffinization question

[sheila adey]

Hello Histonetters.

I am curious about how long other labs are spending in Xylene when bringing 
tissue to water? I started here 6 years ago and never questioned the 
rehydration times but I think we may not be spending enough time in Xylene. 
Our times are 1 min in each of 2 xylenes. This week we increased it to 5 
minutes in each and I think our IHCs are staining better?
Any input?
Thanks

Sheila Adey HT, MLT
Port Huron Michigan

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