From hodges420 <@t> msn.com Tue Aug 1 06:31:42 2006 From: hodges420 <@t> msn.com (MARY T HODGES) Date: Tue Aug 1 06:31:52 2006 Subject: [Histonet] ish Message-ID: Good morning all, can anyone supply me with documents that support the fact that alcohol fixation with ish can cause negative results. my docs want to see it in writting thanks tere hodges st mary's hospital tucson,az From christopher.overend <@t> huskymail.uconn.edu Tue Aug 1 07:26:36 2006 From: christopher.overend <@t> huskymail.uconn.edu (Christopher C Overend) Date: Tue Aug 1 07:26:57 2006 Subject: [Histonet] IFA staining problems Message-ID: <208c20a2088ec8.2088ec8208c20a@huskymail.uconn.edu> My IFA guy fixed most of his smears and sections for 5-10 minutes only in cold acetone (there was one test that needed methanol only and a few oddball tests needed ethanol). You might try reducing your fixation time in acetone. Jan Shivers U of MN Veterinary Diagnostic Lab St. Paul, MN shive003@umn.edu ----- Original Message ----- From: "Christopher C Overend" To: Sent: Monday, July 31, 2006 12:41 PM Subject: [Histonet] IFA staining problems >I am new to the technique of immunofluorescence, and have been doing some >work with it lately on cultured cells. Specifically, I have been staining >intracellular virus with very good results. However, when i try to detect >cellular proteins, I do not get any staining. The proceedure i have been >using consists of "fixation" with 90% acetone for 30 min, at 4degrees C. >the rest of the process had been much like an ELISA, all incubations have >been for 30 min at 4degrees. The buffer i have been using is PBS, 1%BSA, >0.1%NaN3. Someone mentioned the problem could be from the acetone and >suggested trying a glyoxal fixative. Can anyone offer some insights, or if >there is a different protocol i might have to follow using a glyoxal >fixative with tissue culture? > Thank you! > Chris > > Christopher Overend > Ph.D Student > University of Connecticut > Department of Pathobiology and Veterinary Sciences > Storrs, CT > Christopher.overend@uconn.edu From anh2006 <@t> med.cornell.edu Tue Aug 1 08:48:47 2006 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Tue Aug 1 08:49:01 2006 Subject: [Histonet] Acetone for frozen sections and whole mounts In-Reply-To: References: Message-ID: That's interesting and strange as I have never noticed autofluorescence with acetone on frozen sections (I don't personally use acetone for whole mounts so cannot comment on that aspect). In fact, it's normally the opposite, we get horrible autofluorescence from PFA and I am always suggesting to my coworkers to try acetone instead. At 12:28 PM -0500 7/31/06, MVaughan4@ucok.edu wrote: >My experience with acetone on frozen and whole mounts is that there is >often nonspecific fluorescence in the connective tissues. This may not >apply to enzyme IHC but can be bad for fluorescence. Try Ethanol or >Methanol if this happens to you. >Mel >Melville B. Vaughan, Ph. D. >Assistant Professor >Department of Biology >University of Central Oklahoma >100 N. University Drive >Edmond, OK 73034 >http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- From lblazek <@t> digestivespecialists.com Tue Aug 1 09:25:19 2006 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Aug 1 09:20:55 2006 Subject: [Histonet] coverslipper Message-ID: <6CBA6DC98A079D408C87250591D9DFB80233E152@bruexchange.digestivespecialists.com> Does anyone use a xylene substitute with an automatic coverslipper specifically ClearRite 3? The last time I had any dealings with a coverslipper you could only use xylene. Since I'm in the market for one vendors feel free to contact me. Thanks. - Linda Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com From susan.wells <@t> bms.com Tue Aug 1 10:15:19 2006 From: susan.wells <@t> bms.com (Susan Q Wells) Date: Tue Aug 1 10:27:33 2006 Subject: [Histonet] Whole Mount TUNEL Message-ID: <44CF7007.6040406@bms.com> Does anyone have a procedure for whole mount staining using the TUNEL assay? I'm using a rodent model but any input would be appreciated. Thanks, Sue Wells From Melissa.Gonzalez <@t> cellgenesys.com Tue Aug 1 12:18:52 2006 From: Melissa.Gonzalez <@t> cellgenesys.com (Melissa Gonzalez) Date: Tue Aug 1 12:19:00 2006 Subject: [Histonet] RE: Foxp3 on Murine tissue Message-ID: I've also tried several of the products out there with no success on murine tissues as well. I would be interested in hearing if anyone has had success with FoxP3 yet on murine tissues, and what you could use as a positive control. Thanks, Melissa ------------------------------ Message: 7 Date: Mon, 31 Jul 2006 14:02:03 -0500 From: "Drew Allan Roenneburg" Subject: [Histonet] Foxp3 on Murine tissue To: Message-ID: <44CE0D5B0200009C00001F23@gw.surgery.wisc.edu> Content-Type: text/plain; charset=US-ASCII Hi, I was wondering if anyone has stained for foxp3 on frozen or FFPE mouse tissues (spleen). I have tried the Abcam rabbit anti-human foxp3 antibody that works on FFPE human and monkey (tonsil and spleen), however have had little success on mouse. Thanks Drew Roenneburg From PIXLEYSK <@t> UCMAIL.UC.EDU Tue Aug 1 12:45:30 2006 From: PIXLEYSK <@t> UCMAIL.UC.EDU (Pixley, Sarah (pixleysk)) Date: Tue Aug 1 12:45:36 2006 Subject: [Histonet] RE:BrdU and GFP staining Message-ID: Dear Steve: I would stain for GFP with an antibody to GFP and do labeling with DAB. I recommend the Elite ABC kits. The DAB is moderately resistant to the BrdU penetration protocols. Then, fix your tissues well with paraformaldehyde, i.e. 15 mins, 4%, and proceed with the BrdU, using a system that labels with a fluorescent antibody. This will only work, however, if your GFP is in different cells, or it is in the cytoplasm of large cells that have no nuclear staining. The DAB can obscure the secondary fluorescent labeling. Good luck. Stay Cool back at you. Sarah Pixley, Ohio Message: 2 Date: Mon, 31 Jul 2006 10:22:41 -0700 (PDT) From: Steven Coakley Subject: [Histonet] BrdU and GFP expression To: Histonet@lists.utsouthwestern.edu Message-ID: <20060731172241.65699.qmail@web38215.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Good afternoon from WI, We have several FFPE blocks from 2003 that express GFP. Our lead scientist would like to perform Brdu IHC in the same slides hoping to express both. As expected the AR removes the GFP green label. Has anyone any experience expressing Brdu and GFP on the same slide. Stay cool, Steve From PIXLEYSK <@t> UCMAIL.UC.EDU Tue Aug 1 12:49:31 2006 From: PIXLEYSK <@t> UCMAIL.UC.EDU (Pixley, Sarah (pixleysk)) Date: Tue Aug 1 12:49:34 2006 Subject: [Histonet] Staining cultured cells Message-ID: To stain cultured cells we routinely fix with 4% paraformaldehyde for 15-20 mins. But if the antigen is sensitive to aldehydes, then we fix with ice-cold 100% methanol for 5-10 mins. Your acetone technique for 30 mins is extremely harsh for cultured cells. If you want to continue with it, I would fix with the acetone for only a few minutes and try that. As for the other person who asked about staining cultured cells, we do exactly the same ICC as on sections, although we usually are able to dilute the primary antibodies more than with frozen tissue sections (using paraformaldehyde perfused tissues). Sarah Pixley Ohio Message: 4 Date: Mon, 31 Jul 2006 13:41:54 -0400 From: Christopher C Overend Subject: [Histonet] IFA staining problems To: histonet@lists.utsouthwestern.edu Message-ID: <1fbaf901fb5b0e.1fb5b0e1fbaf90@huskymail.uconn.edu> Content-Type: text/plain; charset=us-ascii I am new to the technique of immunofluorescence, and have been doing some work with it lately on cultured cells. Specifically, I have been staining intracellular virus with very good results. However, when i try to detect cellular proteins, I do not get any staining. The proceedure i have been using consists of "fixation" with 90% acetone for 30 min, at 4degrees C. the rest of the process had been much like an ELISA, all incubations have been for 30 min at 4degrees. The buffer i have been using is PBS, 1%BSA, 0.1%NaN3. Someone mentioned the problem could be from the acetone and suggested trying a glyoxal fixative. Can anyone offer some insights, or if there is a different protocol i might have to follow using a glyoxal fixative with tissue culture? Thank you! Chris Christopher Overend Ph.D Student University of Connecticut Department of Pathobiology and Veterinary Sciences Storrs, CT Christopher.overend@uconn.edu From Xilong.Li <@t> UTSouthwestern.edu Tue Aug 1 13:23:06 2006 From: Xilong.Li <@t> UTSouthwestern.edu (Xilong Li) Date: Tue Aug 1 13:23:23 2006 Subject: [Histonet] immunohistochemical analysis for connective tissue Message-ID: <44CF55BA020000F000004E06@swnw124.swmed.edu> Hi, All members, I am looking for a consistent protocol to stain collagen of connective tissues in muscle frozen sections by immunohistochemical analysis including information for primary antibody and second antibody used in the protocols. Thanks in advance. xilong li Dr. Xilong Li Hypertension Division, Internal Medicine University of Texas Southwestern Medical Center 5323 Harry Hiness Blvd-J4.142 Dallas, TX 75390 Tel: 214-648-9966(L) Fax: 214-648-7902 From hej01 <@t> health.state.ny.us Tue Aug 1 13:24:37 2006 From: hej01 <@t> health.state.ny.us (Helen E Johnson) Date: Tue Aug 1 13:24:48 2006 Subject: [Histonet] ImmunoVision Technologies, Co. Message-ID: Hi Histonetters, Does anyone know if ImmunoVision Technologies, Co. is still in business? No one answers the phone ( 650-992-7563). Helen (hej01@health.state.ny.us) From spinquist <@t> yahoo.com Tue Aug 1 13:32:41 2006 From: spinquist <@t> yahoo.com (Ron Lagerquist) Date: Tue Aug 1 13:32:46 2006 Subject: [Histonet] ImmunoVision Technologies, Co. In-Reply-To: Message-ID: <20060801183241.80617.qmail@web56106.mail.re3.yahoo.com> They were purchased by VisionBiosystems (800-753-7264) http://www.vision-bio.com/media_releases.html Ron Helen E Johnson wrote: Hi Histonetters, Does anyone know if ImmunoVision Technologies, Co. is still in business? No one answers the phone ( 650-992-7563). Helen (hej01@health.state.ny.us) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Next-gen email? Have it all with the all-new Yahoo! Mail Beta. From pmarcum <@t> vet.upenn.edu Tue Aug 1 13:33:51 2006 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Tue Aug 1 13:33:57 2006 Subject: [Histonet] Pennsylvania Histology Meeting for October 2006 Message-ID: <6.1.1.1.2.20060801142646.01a26d88@mail.vet.upenn.edu> Good Afternoon All, We have our web site up and partially up. We do have our program online and have the program in the download file area for you to review and if register. The program can be printed off. Just go to www.pahisto.org and look for downloads in the top line. Click on and open or save. The format is a Microsoft Word document. If you have a problem or would need a pre-printing copy please let either me or Gloria Limetti know. Gloria can be reached at glorialimetti@yahoo.com or 412-647-8535. Thanks for your patience and we will be updating the program with vendors and other information as we near the meeting in October. Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From AnthonyH <@t> chw.edu.au Tue Aug 1 19:49:46 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Tue Aug 1 19:51:01 2006 Subject: [Histonet] Ethanol/other fixations for frozen sections Message-ID: Urgent Frozen Sections for H&E - we fix in methanol Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea T. Hooper Sent: Monday, 31 July 2006 10:45 PM To: Histonet Subject: [Histonet] Ethanol/other fixations for frozen sections Just taking a survey .... What are everyone's thoughts on using ethanol for frozen sections? I have a colleague who swears by it but I am an acetone person myself. Thoughts, comments, input on what is the best fix for frozen section IHC? Thanks! -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From tkngflght <@t> yahoo.com Tue Aug 1 21:40:34 2006 From: tkngflght <@t> yahoo.com (Cheryl) Date: Tue Aug 1 21:40:37 2006 Subject: [Histonet] Looking for Florida histotechs--or people who are interested in Florida relocation: we help with the license. In-Reply-To: Message-ID: <20060802024034.14424.qmail@web50904.mail.yahoo.com> Hello everyone-- Florida's registry process has created a major shortage of qualified techs in the state. We have a number of Florida permanent and temp opportunities. The permanent labs will consider transitional job situations to allow you to work while your license is processed. We also have a number of temp situations for a great pay scale for techs registered to work in the state. Travel isn't for everyone, but these are good labs and a great way to start. Either way, we'll help you through the process to get you through it as quickly as possible. If you're even curious, give us a call. Of course we have a number of other opportunities including sales and management all over the country. We won't work with every lab--those we place find themselves in jobs that fit--where they are happy to go to work. We're here to help and no fee to you. Tiffany is in the office and I'm traveling as a temp for a couple of weeks--as always, referral bonuses are available--share us with your friends! Tiffany/Office 281.852.9457 Cheryl/Cell 281.883.7704 or this number follows me around: 800.756.3309 (it will ring a long time to find me--please be patient and leave your phone number!) We return all calls. Thanks! Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one Tech at a time. 281.883.7704 c 281.852.9457 o admin@fullstaff.org Sign up for the FREE monthly newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. From louise.renton <@t> gmail.com Wed Aug 2 02:47:06 2006 From: louise.renton <@t> gmail.com (louise renton) Date: Wed Aug 2 02:47:13 2006 Subject: [Histonet] article from J of histotechnology Message-ID: Hi can anyone share a copy of this article? I would be very grateful "Entering the realm of mineralized bone processing: a review of the literature and techniques." J Histotechnol 1997:20 (3) p259-266 Many thanks Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. From Shirley_PHUA <@t> hsa.gov.sg Wed Aug 2 03:19:02 2006 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Wed Aug 2 03:19:41 2006 Subject: [Histonet] Zinc-Based Fixative Message-ID: Dear All, Reference Article: Lab Invest 2003 Jun;83(6):889-99 Zinc-based fixative improves preservation of genomic DNA and proteins in histoprocessing of human tissues Anyone out there know of the exact composition of any zinc-based fixative that is based on the following? 1. 0.5% Zinc Chloride and 2. 0.5% Zinc Acetate in 0.1M Tris base buffer containing 0.05% calcium acetate Many thanks & regards, Shirley Phua Histopathology Laboratory Centre for Forensic Medicine Health Sciences Authority Singapore From Vickroy.Jim <@t> mhsil.com Wed Aug 2 08:23:30 2006 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Wed Aug 2 08:23:35 2006 Subject: [Histonet] prefilled formalin containers Message-ID: We have experienced problems with prefilled formalin containers that we send to our clients. We have tried two different vendors and they both leak. One of the container types claims to have an o-ring type lid and the other does not have an o-ring. The containers with the o-ring seems to leak as much as the others. In fact the o-ring type sometimes leak when they are received from the vendor. If anyone has dealt with this successfully please let me know how you solved it. Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From Jackie.O'Connor <@t> abbott.com Wed Aug 2 08:41:32 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed Aug 2 08:42:03 2006 Subject: [Histonet] prefilled formalin containers In-Reply-To: Message-ID: Are they leaking on the return trip? Hard to get your clients to make sure they are screwed on properly. Each vial/jar should be contained within a secondary leakproof bag, anyway - to prevent leaks in transit. Give Surgipath a call. Jackie O' "Vickroy, Jim" Sent by: histonet-bounces@lists.utsouthwestern.edu 08/02/2006 08:23 AM To cc Subject [Histonet] prefilled formalin containers We have experienced problems with prefilled formalin containers that we send to our clients. We have tried two different vendors and they both leak. One of the container types claims to have an o-ring type lid and the other does not have an o-ring. The containers with the o-ring seems to leak as much as the others. In fact the o-ring type sometimes leak when they are received from the vendor. If anyone has dealt with this successfully please let me know how you solved it. Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From froyer <@t> bitstream.net Wed Aug 2 08:42:33 2006 From: froyer <@t> bitstream.net (Ford Royer) Date: Wed Aug 2 08:42:41 2006 Subject: [Histonet] '07 TSH Meeting In-Reply-To: Message-ID: <003101c6b639$82553090$7701a80a@Ford> Would the person(s) who are organizing next years Texas Society of Histotechnology meeting please contact me? Thanks! ~ Ford Ford M. Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL: 612-839-1046 Phone: 763-542-8725 Fax: 763-546-4830 eMail: froyer@bitstream.net From dsantana <@t> pmaonline.com Wed Aug 2 09:05:40 2006 From: dsantana <@t> pmaonline.com (Santana, Diane) Date: Wed Aug 2 09:28:04 2006 Subject: [Histonet] The New VIP Message-ID: <4C96AA62BADFD81180CB0002A5AD67FB07113AC5@MAILPMA> I have a question and please nobody get all defensive about this, but has anybody recently bought the VIP 5 tissue processor? If you have, do or did you have any problems with it? Thanks Diane Santana PMA Haverhill, Mass. From funderwood <@t> mcohio.org Wed Aug 2 09:31:26 2006 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Wed Aug 2 09:31:51 2006 Subject: [Histonet] The New VIP Message-ID: I've had mine for a year and (knock, knock on wood) have had no problems. Fred >>> "Santana, Diane" 08/02 10:05 AM >>> I have a question and please nobody get all defensive about this, but has anybody recently bought the VIP 5 tissue processor? If you have, do or did you have any problems with it? Thanks Diane Santana PMA Haverhill, Mass. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Charlene.Henry <@t> STJUDE.ORG Wed Aug 2 09:36:48 2006 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Wed Aug 2 09:36:52 2006 Subject: [Histonet] The New VIP. . Message-ID: <5CB39BCA5724F349BCB748675C6CA1A2099A1D6A@SJMEMXMB02.stjude.sjcrh.local> We purchased a VIP5 a few years ago and had several problems so it was replaced with a new VIP5. Since that time we have had no problems and it has been a reliable instrument. Charlene -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Santana, Diane Sent: Wednesday, August 02, 2006 9:06 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] The New VIP. . I have a question and please nobody get all defensive about this, but has anybody recently bought the VIP 5 tissue processor? If you have, do or did you have any problems with it? Thanks Diane Santana PMA Haverhill, Mass. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From crains <@t> wpmpath.com Wed Aug 2 10:30:16 2006 From: crains <@t> wpmpath.com (crains@wpmpath.com) Date: Wed Aug 2 10:30:21 2006 Subject: [Histonet] The New VIP Message-ID: <20060802153018.PMMJ26069.dukecmmtao03.coxmail.com@dukecmmtao03> We have a VIP 5 that we purchased a little over a year ago. Have not had a single problem with it so far. Chris Rains WPM Pathology Laboratory Salina, KS > > From: "Santana, Diane" > Date: 2006/08/02 Wed AM 09:05:40 CDT > To: "'histonet@lists.utsouthwestern.edu'" > Subject: [Histonet] The New VIP > > I have a question and please nobody get all defensive about this, but has > anybody recently bought the VIP 5 tissue processor? If you have, do or did > you have any problems with it? > Thanks > Diane Santana > PMA > Haverhill, Mass. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Important: This email and any attachments may contain confidential information subject to protection under the Federal Standards for Privacy of Individually Identifiable Health Information (45 C.F.R. Parts 160 and 164). If you or your organization is a "Covered Entity" under the above mentioned regulations, you are obligated to treat such information in a manner consistent with the regulations. If it appears that this email was sent to you in error, (1) you are prohibited from utilizing or disseminating this email or any attachments; (2) please immediately delete it from your computer and any servers or other locations where it might be stored and email (crains@wpmpath.com) or call (Chris Rains) at 785-823-7201 advising that you have done so. We appreciate your cooperation. From mstahl <@t> bvha.org Wed Aug 2 10:42:17 2006 From: mstahl <@t> bvha.org (Stahl, Michael) Date: Wed Aug 2 10:42:21 2006 Subject: [Histonet] prefilled formalin containers Message-ID: <4C878E714B21EB4F8EB159777B8822EE5B6834@bvfyms01.net.bvha.org> We use a Surgipath (cat #008100 which are the pre-filled 60ml jars. We found these to be almost leak proof. However, they do leak when the jars arnt screwed on correctly. But we agree, its not pleasant to have a specimen jar in a bio hazard bag full of fixative. Just try to find that cervial biospy! Michael P. Stahl HT (ASCP) BVRHC 145 W Wallace St Findlay, OH 45840 mstahl@bhva.org From algranth <@t> u.arizona.edu Wed Aug 2 10:55:45 2006 From: algranth <@t> u.arizona.edu (Andrea Grantham) Date: Wed Aug 2 10:55:53 2006 Subject: [Histonet] The New VIP In-Reply-To: <20060802153018.PMMJ26069.dukecmmtao03.coxmail.com@dukecmmt ao03> Message-ID: <4.3.2.7.2.20060802085142.00cec460@algranth.inbox.email.arizona.edu> I just got a new VIP 5 and I love it. Wanted one all my (histotech) life and I'm so happy to finally have it! It has been great. So easy to use and the tissues are coming out wonderful. Knock-on-wood it will do as well as the old Tissue Tek dipper/dunker that it is replacing. Andi At 10:30 AM 8/2/2006 -0500, crains@wpmpath.com wrote: >We have a VIP 5 that we purchased a little over a year ago. Have not had >a single problem with it so far. > >Chris Rains >WPM Pathology Laboratory >Salina, KS > > > > From: "Santana, Diane" > > Date: 2006/08/02 Wed AM 09:05:40 CDT > > To: "'histonet@lists.utsouthwestern.edu'" > > > Subject: [Histonet] The New VIP > > > > I have a question and please nobody get all defensive about this, but has > > anybody recently bought the VIP 5 tissue processor? If you have, do or did > > you have any problems with it? > > Thanks > > Diane Santana > > PMA > > Haverhill, Mass. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >Important: This email and any attachments may contain confidential >information subject to protection under the Federal Standards for Privacy >of Individually Identifiable Health Information (45 C.F.R. Parts 160 and >164). If you or your organization is a "Covered Entity" under the above >mentioned regulations, you are obligated to treat such information in a >manner consistent with the regulations. If it appears that this email was >sent to you in error, (1) you are prohibited from utilizing or >disseminating this email or any attachments; (2) please immediately delete >it from your computer and any servers or other locations where it might be >stored and email (crains@wpmpath.com) or call (Chris Rains) at >785-823-7201 advising that you have done so. We appreciate your cooperation. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html From Vickroy.Jim <@t> mhsil.com Wed Aug 2 11:08:00 2006 From: Vickroy.Jim <@t> mhsil.com (Vickroy, Jim) Date: Wed Aug 2 11:08:36 2006 Subject: [Histonet] charging and CPT questions Message-ID: Can anyone shed any light on the proper way to code and charge the following: 1. Muscle Biopsy Histochemistry - Frozen sections -88314 or 88319? I'm not sure which one. 2. Touch preps - Done when we do a frozen section. The frozen section is 88331 and the touch prep should be 88333 or 88334? 3. Semi-thin sectioning of nerve biopsies - Plastic embedding In the past we charged all nerve bxs with an 88348 (EM) charge since not only did we do the semi-thin sectioning but we also did the transmission EM. Our neuropathologist now wants the semi-thin sections routinely but not the rest of the EM examination. Thanks Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. From jscoggin <@t> biocept.com Wed Aug 2 11:45:21 2006 From: jscoggin <@t> biocept.com (Jayne Scoggin) Date: Wed Aug 2 11:24:07 2006 Subject: [Histonet] please unsubscribe me from the list Message-ID: <200608021624.k72GO3Vh004624@mx.bioceptlabs.com> Please unsubscribe me from the list. Jayne Scoggin, Biocept Inc. 858 320 8224 jscoggin@biocept.com From Kari.Zajic <@t> HCAhealthcare.com Wed Aug 2 11:40:55 2006 From: Kari.Zajic <@t> HCAhealthcare.com (Zajic Kari) Date: Wed Aug 2 11:42:12 2006 Subject: [Histonet] charging and CPT questions In-Reply-To: Message-ID: <095327C7CDBDF64B9E9728A54799091E015CC5A2@ORLEV03.hca.corpad.net> Hi Jim. I do not do muscle or nerve at my facility but we send to the University of Miami and they charge the following: Muscle 88305 x1: Surgical Path,gross,micro 88313 x2: Paraffin: H&E Plastic 88314 x2: cryostat H&E Trichrome 88319 x8: Enzyme HistoChem: NADH,Esterase, ATPasex3,Cox, Cox & SDH, SDH Nerve 88305 x1: Surg Path,gross,micro 88313 x6: Paraffin H&E,Trichrome,Luxol Fast Blue,Toludine Blue, Crystal Violet,Silver Stain 88314 x2: Cryostat: H&E,Trichrome 88319 x1: Frozen Section Histochem: Esterase 88348 x1: Nerve Electron Micro 88356 x1: Nerve Morphometry 88362 x1: Nerve Teased Preps As far as frozen section touch preps, they came out with new CPT's in 2006 as follows: 88333 Path consult during surgery (cyto exam) initial site ie.touch prep,squash prep physician fee global $75-100+ technical $17-25 88334 cyto exam, EACH ADDITIONAL SITE, touch prep,squash prep physicain fee $40-50+ technical $10-15 this info was given to me by Health Systems Concepts,Inc. hope this helps!!! Kari :) Kari Marie Zajic HT,MLT Histology Supervisor Palms West Hospital Pathology Department 13001 State Road Eighty Loxahatchee, Florida 33470 phone: (561)798-6036 telefax: (561)753-4298 voicemail: (561)753-4299 pager: (561)610-4949 email: Kari.Zajic@HCAHealthcare.com This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Vickroy, Jim Sent: Wednesday, August 02, 2006 12:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] charging and CPT questions Can anyone shed any light on the proper way to code and charge the following: 1. Muscle Biopsy Histochemistry - Frozen sections -88314 or 88319? I'm not sure which one. 2. Touch preps - Done when we do a frozen section. The frozen section is 88331 and the touch prep should be 88333 or 88334? 3. Semi-thin sectioning of nerve biopsies - Plastic embedding In the past we charged all nerve bxs with an 88348 (EM) charge since not only did we do the semi-thin sectioning but we also did the transmission EM. Our neuropathologist now wants the semi-thin sections routinely but not the rest of the EM examination. Thanks Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mhannah <@t> jhsph.edu Wed Aug 2 12:25:53 2006 From: mhannah <@t> jhsph.edu (Hannah, Michele F.) Date: Wed Aug 2 12:28:37 2006 Subject: [Histonet] endothelial cell antibody References: <57000j$1fh54p@gateway1.jhsph.edu> Message-ID: <1D71A10BB247204A9EFFB9EED3236058019168CE@XCH-VN02.sph.ad.jhsph.edu> Has anyone worked with an antibody to endothelial cells that has worked well for them? If appropriate, was it successful in lung tissue and/or what were the conditions (FFPE, frozen, ect)? I am looking at several different possibilities and wanted to know what other people were using. Thanks! Michele Michele F. Hannah M.S. Department of Molecular Microbiology & Immunology Johns Hopkins Bloomberg School of Public Health 615 North Wolfe Street Room E3201 Baltimore, Maryland 21205 Phone: (410) 614-7794 Fax: (410) 955-0105 From MadaryJ <@t> MedImmune.com Wed Aug 2 12:42:01 2006 From: MadaryJ <@t> MedImmune.com (Madary, Joseph) Date: Wed Aug 2 12:42:19 2006 Subject: [Histonet] VIP 5 In-Reply-To: Message-ID: <7CAB706201F11843BD26AD516326F0C801228AF1@MD1MS007.medimmune.com> Just got it a couple of months ago and so far I cannot complain too much. I have a couple of items I think are a bit backwards like I cannot enter in how many cassettes I ran, I can only print out run info if I want, I cannot turn off the paraffin if I want to save heating it for a week if I am not running anything. I know that if you do not stick with the maintenance it is funny, especially the warm water flushes. Honestly I like what comes out at the end but I expected a much more advanced product in terms of software. I would buy another though.... I was using a dip and dunk so anything was better than that Thermo Shandon Citadel 1000. I mean it is so unhealthy to have those around, they should not be on the market. What a difference in the lab fumes when we switched. I mean I used a dip and dunk in 1979, and could not believe it when I got here and had to use one for a couple of years. All good now though. Okay, probably in trouble with someone now. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Wednesday, August 02, 2006 1:13 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 33, Issue 2 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Foxp3 on Murine tissue (Melissa Gonzalez) 2. RE:BrdU and GFP staining (Pixley, Sarah (pixleysk)) 3. Staining cultured cells (Pixley, Sarah (pixleysk)) 4. immunohistochemical analysis for connective tissue (Xilong Li) 5. ImmunoVision Technologies, Co. (Helen E Johnson) 6. Re: ImmunoVision Technologies, Co. (Ron Lagerquist) 7. Pennsylvania Histology Meeting for October 2006 (Pamela Marcum) 8. RE: Ethanol/other fixations for frozen sections (Tony Henwood) 9. Looking for Florida histotechs--or people who are interested in Florida relocation: we help with the license. (Cheryl) 10. article from J of histotechnology (louise renton) 11. Zinc-Based Fixative (Shirley PHUA) 12. prefilled formalin containers (Vickroy, Jim) 13. Re: prefilled formalin containers (Jackie M O'Connor) 14. '07 TSH Meeting (Ford Royer) 15. The New VIP (Santana, Diane) 16. Re: The New VIP (Fred Underwood) 17. RE: The New VIP. . (Henry, Charlene) 18. Re: The New VIP (crains@wpmpath.com) 19. prefilled formalin containers (Stahl, Michael) 20. Re: The New VIP (Andrea Grantham) 21. charging and CPT questions (Vickroy, Jim) 22. please unsubscribe me from the list (Jayne Scoggin) 23. RE: charging and CPT questions (Zajic Kari) ---------------------------------------------------------------------- Message: 1 Date: Tue, 1 Aug 2006 10:18:52 -0700 From: "Melissa Gonzalez" Subject: [Histonet] RE: Foxp3 on Murine tissue To: Message-ID: Content-Type: text/plain; charset="us-ascii" I've also tried several of the products out there with no success on murine tissues as well. I would be interested in hearing if anyone has had success with FoxP3 yet on murine tissues, and what you could use as a positive control. Thanks, Melissa ------------------------------ Message: 7 Date: Mon, 31 Jul 2006 14:02:03 -0500 From: "Drew Allan Roenneburg" Subject: [Histonet] Foxp3 on Murine tissue To: Message-ID: <44CE0D5B0200009C00001F23@gw.surgery.wisc.edu> Content-Type: text/plain; charset=US-ASCII Hi, I was wondering if anyone has stained for foxp3 on frozen or FFPE mouse tissues (spleen). I have tried the Abcam rabbit anti-human foxp3 antibody that works on FFPE human and monkey (tonsil and spleen), however have had little success on mouse. Thanks Drew Roenneburg ------------------------------ Message: 2 Date: Tue, 1 Aug 2006 13:45:30 -0400 From: "Pixley, Sarah \(pixleysk\)" Subject: [Histonet] RE:BrdU and GFP staining To: Message-ID: Content-Type: text/plain; charset="us-ascii" Dear Steve: I would stain for GFP with an antibody to GFP and do labeling with DAB. I recommend the Elite ABC kits. The DAB is moderately resistant to the BrdU penetration protocols. Then, fix your tissues well with paraformaldehyde, i.e. 15 mins, 4%, and proceed with the BrdU, using a system that labels with a fluorescent antibody. This will only work, however, if your GFP is in different cells, or it is in the cytoplasm of large cells that have no nuclear staining. The DAB can obscure the secondary fluorescent labeling. Good luck. Stay Cool back at you. Sarah Pixley, Ohio Message: 2 Date: Mon, 31 Jul 2006 10:22:41 -0700 (PDT) From: Steven Coakley Subject: [Histonet] BrdU and GFP expression To: Histonet@lists.utsouthwestern.edu Message-ID: <20060731172241.65699.qmail@web38215.mail.mud.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Good afternoon from WI, We have several FFPE blocks from 2003 that express GFP. Our lead scientist would like to perform Brdu IHC in the same slides hoping to express both. As expected the AR removes the GFP green label. Has anyone any experience expressing Brdu and GFP on the same slide. Stay cool, Steve ------------------------------ Message: 3 Date: Tue, 1 Aug 2006 13:49:31 -0400 From: "Pixley, Sarah \(pixleysk\)" Subject: [Histonet] Staining cultured cells To: Message-ID: Content-Type: text/plain; charset="us-ascii" To stain cultured cells we routinely fix with 4% paraformaldehyde for 15-20 mins. But if the antigen is sensitive to aldehydes, then we fix with ice-cold 100% methanol for 5-10 mins. Your acetone technique for 30 mins is extremely harsh for cultured cells. If you want to continue with it, I would fix with the acetone for only a few minutes and try that. As for the other person who asked about staining cultured cells, we do exactly the same ICC as on sections, although we usually are able to dilute the primary antibodies more than with frozen tissue sections (using paraformaldehyde perfused tissues). Sarah Pixley Ohio Message: 4 Date: Mon, 31 Jul 2006 13:41:54 -0400 From: Christopher C Overend Subject: [Histonet] IFA staining problems To: histonet@lists.utsouthwestern.edu Message-ID: <1fbaf901fb5b0e.1fb5b0e1fbaf90@huskymail.uconn.edu> Content-Type: text/plain; charset=us-ascii I am new to the technique of immunofluorescence, and have been doing some work with it lately on cultured cells. Specifically, I have been staining intracellular virus with very good results. However, when i try to detect cellular proteins, I do not get any staining. The proceedure i have been using consists of "fixation" with 90% acetone for 30 min, at 4degrees C. the rest of the process had been much like an ELISA, all incubations have been for 30 min at 4degrees. The buffer i have been using is PBS, 1%BSA, 0.1%NaN3. Someone mentioned the problem could be from the acetone and suggested trying a glyoxal fixative. Can anyone offer some insights, or if there is a different protocol i might have to follow using a glyoxal fixative with tissue culture? Thank you! Chris Christopher Overend Ph.D Student University of Connecticut Department of Pathobiology and Veterinary Sciences Storrs, CT Christopher.overend@uconn.edu ------------------------------ Message: 4 Date: Tue, 01 Aug 2006 13:23:06 -0500 From: "Xilong Li" Subject: [Histonet] immunohistochemical analysis for connective tissue To: Message-ID: <44CF55BA020000F000004E06@swnw124.swmed.edu> Content-Type: text/plain; charset=US-ASCII Hi, All members, I am looking for a consistent protocol to stain collagen of connective tissues in muscle frozen sections by immunohistochemical analysis including information for primary antibody and second antibody used in the protocols. Thanks in advance. xilong li Dr. Xilong Li Hypertension Division, Internal Medicine University of Texas Southwestern Medical Center 5323 Harry Hiness Blvd-J4.142 Dallas, TX 75390 Tel: 214-648-9966(L) Fax: 214-648-7902 ------------------------------ Message: 5 Date: Tue, 1 Aug 2006 14:24:37 -0400 From: Helen E Johnson Subject: [Histonet] ImmunoVision Technologies, Co. To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=US-ASCII Hi Histonetters, Does anyone know if ImmunoVision Technologies, Co. is still in business? No one answers the phone ( 650-992-7563). Helen (hej01@health.state.ny.us) ------------------------------ Message: 6 Date: Tue, 1 Aug 2006 11:32:41 -0700 (PDT) From: Ron Lagerquist Subject: Re: [Histonet] ImmunoVision Technologies, Co. To: Helen E Johnson , histonet@lists.utsouthwestern.edu Message-ID: <20060801183241.80617.qmail@web56106.mail.re3.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 They were purchased by VisionBiosystems (800-753-7264) http://www.vision-bio.com/media_releases.html Ron Helen E Johnson wrote: Hi Histonetters, Does anyone know if ImmunoVision Technologies, Co. is still in business? No one answers the phone ( 650-992-7563). Helen (hej01@health.state.ny.us) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Next-gen email? Have it all with the all-new Yahoo! Mail Beta. ------------------------------ Message: 7 Date: Tue, 01 Aug 2006 14:33:51 -0400 From: Pamela Marcum Subject: [Histonet] Pennsylvania Histology Meeting for October 2006 To: histonet@lists.utsouthwestern.edu Message-ID: <6.1.1.1.2.20060801142646.01a26d88@mail.vet.upenn.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed Good Afternoon All, We have our web site up and partially up. We do have our program online and have the program in the download file area for you to review and if register. The program can be printed off. Just go to www.pahisto.org and look for downloads in the top line. Click on and open or save. The format is a Microsoft Word document. If you have a problem or would need a pre-printing copy please let either me or Gloria Limetti know. Gloria can be reached at glorialimetti@yahoo.com or 412-647-8535. Thanks for your patience and we will be updating the program with vendors and other information as we near the meeting in October. Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu ------------------------------ Message: 8 Date: Wed, 2 Aug 2006 10:49:46 +1000 From: "Tony Henwood" Subject: RE: [Histonet] Ethanol/other fixations for frozen sections To: "Andrea T. Hooper" , "Histonet" Message-ID: Content-Type: text/plain; charset="us-ascii" Urgent Frozen Sections for H&E - we fix in methanol Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea T. Hooper Sent: Monday, 31 July 2006 10:45 PM To: Histonet Subject: [Histonet] Ethanol/other fixations for frozen sections Just taking a survey .... What are everyone's thoughts on using ethanol for frozen sections? I have a colleague who swears by it but I am an acetone person myself. Thoughts, comments, input on what is the best fix for frozen section IHC? Thanks! -- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** ------------------------------ Message: 9 Date: Tue, 1 Aug 2006 19:40:34 -0700 (PDT) From: Cheryl Subject: [Histonet] Looking for Florida histotechs--or people who are interested in Florida relocation: we help with the license. To: Histonet Message-ID: <20060802024034.14424.qmail@web50904.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Hello everyone-- Florida's registry process has created a major shortage of qualified techs in the state. We have a number of Florida permanent and temp opportunities. The permanent labs will consider transitional job situations to allow you to work while your license is processed. We also have a number of temp situations for a great pay scale for techs registered to work in the state. Travel isn't for everyone, but these are good labs and a great way to start. Either way, we'll help you through the process to get you through it as quickly as possible. If you're even curious, give us a call. Of course we have a number of other opportunities including sales and management all over the country. We won't work with every lab--those we place find themselves in jobs that fit--where they are happy to go to work. We're here to help and no fee to you. Tiffany is in the office and I'm traveling as a temp for a couple of weeks--as always, referral bonuses are available--share us with your friends! Tiffany/Office 281.852.9457 Cheryl/Cell 281.883.7704 or this number follows me around: 800.756.3309 (it will ring a long time to find me--please be patient and leave your phone number!) We return all calls. Thanks! Cheryl Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one Tech at a time. 281.883.7704 c 281.852.9457 o admin@fullstaff.org Sign up for the FREE monthly newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. ------------------------------ Message: 10 Date: Wed, 2 Aug 2006 09:47:06 +0200 From: "louise renton" Subject: [Histonet] article from J of histotechnology To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hi can anyone share a copy of this article? I would be very grateful "Entering the realm of mineralized bone processing: a review of the literature and techniques." J Histotechnol 1997:20 (3) p259-266 Many thanks Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ------------------------------ Message: 11 Date: Wed, 2 Aug 2006 16:19:02 +0800 From: Shirley PHUA Subject: [Histonet] Zinc-Based Fixative To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Dear All, Reference Article: Lab Invest 2003 Jun;83(6):889-99 Zinc-based fixative improves preservation of genomic DNA and proteins in histoprocessing of human tissues Anyone out there know of the exact composition of any zinc-based fixative that is based on the following? 1. 0.5% Zinc Chloride and 2. 0.5% Zinc Acetate in 0.1M Tris base buffer containing 0.05% calcium acetate Many thanks & regards, Shirley Phua Histopathology Laboratory Centre for Forensic Medicine Health Sciences Authority Singapore ------------------------------ Message: 12 Date: Wed, 2 Aug 2006 08:23:30 -0500 From: "Vickroy, Jim" Subject: [Histonet] prefilled formalin containers To: Message-ID: Content-Type: text/plain; charset="us-ascii" We have experienced problems with prefilled formalin containers that we send to our clients. We have tried two different vendors and they both leak. One of the container types claims to have an o-ring type lid and the other does not have an o-ring. The containers with the o-ring seems to leak as much as the others. In fact the o-ring type sometimes leak when they are received from the vendor. If anyone has dealt with this successfully please let me know how you solved it. Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ------------------------------ Message: 13 Date: Wed, 2 Aug 2006 08:41:32 -0500 From: "Jackie M O'Connor" Subject: Re: [Histonet] prefilled formalin containers To: "Vickroy, Jim" Cc: histonet@lists.utsouthwestern.edu, histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Are they leaking on the return trip? Hard to get your clients to make sure they are screwed on properly. Each vial/jar should be contained within a secondary leakproof bag, anyway - to prevent leaks in transit. Give Surgipath a call. Jackie O' "Vickroy, Jim" Sent by: histonet-bounces@lists.utsouthwestern.edu 08/02/2006 08:23 AM To cc Subject [Histonet] prefilled formalin containers We have experienced problems with prefilled formalin containers that we send to our clients. We have tried two different vendors and they both leak. One of the container types claims to have an o-ring type lid and the other does not have an o-ring. The containers with the o-ring seems to leak as much as the others. In fact the o-ring type sometimes leak when they are received from the vendor. If anyone has dealt with this successfully please let me know how you solved it. Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 14 Date: Wed, 2 Aug 2006 08:42:33 -0500 From: "Ford Royer" Subject: [Histonet] '07 TSH Meeting To: Message-ID: <003101c6b639$82553090$7701a80a@Ford> Content-Type: text/plain; charset="us-ascii" Would the person(s) who are organizing next years Texas Society of Histotechnology meeting please contact me? Thanks! ~ Ford Ford M. Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL: 612-839-1046 Phone: 763-542-8725 Fax: 763-546-4830 eMail: froyer@bitstream.net ------------------------------ Message: 15 Date: Wed, 2 Aug 2006 10:05:40 -0400 From: "Santana, Diane" Subject: [Histonet] The New VIP To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <4C96AA62BADFD81180CB0002A5AD67FB07113AC5@MAILPMA> Content-Type: text/plain; charset="iso-8859-1" I have a question and please nobody get all defensive about this, but has anybody recently bought the VIP 5 tissue processor? If you have, do or did you have any problems with it? Thanks Diane Santana PMA Haverhill, Mass. ------------------------------ Message: 16 Date: Wed, 02 Aug 2006 10:31:26 -0400 From: "Fred Underwood" Subject: Re: [Histonet] The New VIP To: , Message-ID: Content-Type: text/plain; charset=US-ASCII I've had mine for a year and (knock, knock on wood) have had no problems. Fred >>> "Santana, Diane" 08/02 10:05 AM >>> I have a question and please nobody get all defensive about this, but has anybody recently bought the VIP 5 tissue processor? If you have, do or did you have any problems with it? Thanks Diane Santana PMA Haverhill, Mass. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 17 Date: Wed, 2 Aug 2006 09:36:48 -0500 From: "Henry, Charlene" Subject: RE: [Histonet] The New VIP. . To: "Santana, Diane" , Message-ID: <5CB39BCA5724F349BCB748675C6CA1A2099A1D6A@SJMEMXMB02.stjude.sjcrh.local> Content-Type: text/plain; charset="US-ASCII" We purchased a VIP5 a few years ago and had several problems so it was replaced with a new VIP5. Since that time we have had no problems and it has been a reliable instrument. Charlene -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Santana, Diane Sent: Wednesday, August 02, 2006 9:06 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] The New VIP. . I have a question and please nobody get all defensive about this, but has anybody recently bought the VIP 5 tissue processor? If you have, do or did you have any problems with it? Thanks Diane Santana PMA Haverhill, Mass. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 18 Date: Wed, 2 Aug 2006 10:30:16 -0500 From: Subject: Re: [Histonet] The New VIP To: "Santana, Diane" , "'histonet@lists.utsouthwestern.edu'" Message-ID: <20060802153018.PMMJ26069.dukecmmtao03.coxmail.com@dukecmmtao03> Content-Type: text/plain; charset=ISO-8859-1 We have a VIP 5 that we purchased a little over a year ago. Have not had a single problem with it so far. Chris Rains WPM Pathology Laboratory Salina, KS > > From: "Santana, Diane" > Date: 2006/08/02 Wed AM 09:05:40 CDT > To: "'histonet@lists.utsouthwestern.edu'" > Subject: [Histonet] The New VIP > > I have a question and please nobody get all defensive about this, but has > anybody recently bought the VIP 5 tissue processor? If you have, do or did > you have any problems with it? > Thanks > Diane Santana > PMA > Haverhill, Mass. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > Important: This email and any attachments may contain confidential information subject to protection under the Federal Standards for Privacy of Individually Identifiable Health Information (45 C.F.R. Parts 160 and 164). If you or your organization is a "Covered Entity" under the above mentioned regulations, you are obligated to treat such information in a manner consistent with the regulations. If it appears that this email was sent to you in error, (1) you are prohibited from utilizing or disseminating this email or any attachments; (2) please immediately delete it from your computer and any servers or other locations where it might be stored and email (crains@wpmpath.com) or call (Chris Rains) at 785-823-7201 advising that you have done so. We appreciate your cooperation. ------------------------------ Message: 19 Date: Wed, 2 Aug 2006 11:42:17 -0400 From: "Stahl, Michael" Subject: [Histonet] prefilled formalin containers To: Message-ID: <4C878E714B21EB4F8EB159777B8822EE5B6834@bvfyms01.net.bvha.org> Content-Type: text/plain; charset="us-ascii" We use a Surgipath (cat #008100 which are the pre-filled 60ml jars. We found these to be almost leak proof. However, they do leak when the jars arnt screwed on correctly. But we agree, its not pleasant to have a specimen jar in a bio hazard bag full of fixative. Just try to find that cervial biospy! Michael P. Stahl HT (ASCP) BVRHC 145 W Wallace St Findlay, OH 45840 mstahl@bhva.org ------------------------------ Message: 20 Date: Wed, 02 Aug 2006 08:55:45 -0700 From: Andrea Grantham Subject: Re: [Histonet] The New VIP To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <4.3.2.7.2.20060802085142.00cec460@algranth.inbox.email.arizona.edu> Content-Type: text/plain; charset="us-ascii"; format=flowed I just got a new VIP 5 and I love it. Wanted one all my (histotech) life and I'm so happy to finally have it! It has been great. So easy to use and the tissues are coming out wonderful. Knock-on-wood it will do as well as the old Tissue Tek dipper/dunker that it is replacing. Andi At 10:30 AM 8/2/2006 -0500, crains@wpmpath.com wrote: >We have a VIP 5 that we purchased a little over a year ago. Have not had >a single problem with it so far. > >Chris Rains >WPM Pathology Laboratory >Salina, KS > > > > From: "Santana, Diane" > > Date: 2006/08/02 Wed AM 09:05:40 CDT > > To: "'histonet@lists.utsouthwestern.edu'" > > > Subject: [Histonet] The New VIP > > > > I have a question and please nobody get all defensive about this, but has > > anybody recently bought the VIP 5 tissue processor? If you have, do or did > > you have any problems with it? > > Thanks > > Diane Santana > > PMA > > Haverhill, Mass. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > >Important: This email and any attachments may contain confidential >information subject to protection under the Federal Standards for Privacy >of Individually Identifiable Health Information (45 C.F.R. Parts 160 and >164). If you or your organization is a "Covered Entity" under the above >mentioned regulations, you are obligated to treat such information in a >manner consistent with the regulations. If it appears that this email was >sent to you in error, (1) you are prohibited from utilizing or >disseminating this email or any attachments; (2) please immediately delete >it from your computer and any servers or other locations where it might be >stored and email (crains@wpmpath.com) or call (Chris Rains) at >785-823-7201 advising that you have done so. We appreciate your cooperation. > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html ------------------------------ Message: 21 Date: Wed, 2 Aug 2006 11:08:00 -0500 From: "Vickroy, Jim" Subject: [Histonet] charging and CPT questions To: Message-ID: Content-Type: text/plain; charset="us-ascii" Can anyone shed any light on the proper way to code and charge the following: 1. Muscle Biopsy Histochemistry - Frozen sections -88314 or 88319? I'm not sure which one. 2. Touch preps - Done when we do a frozen section. The frozen section is 88331 and the touch prep should be 88333 or 88334? 3. Semi-thin sectioning of nerve biopsies - Plastic embedding In the past we charged all nerve bxs with an 88348 (EM) charge since not only did we do the semi-thin sectioning but we also did the transmission EM. Our neuropathologist now wants the semi-thin sections routinely but not the rest of the EM examination. Thanks Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. ------------------------------ Message: 22 Date: Wed, 2 Aug 2006 09:45:21 -0700 From: "Jayne Scoggin" Subject: [Histonet] please unsubscribe me from the list To: Message-ID: <200608021624.k72GO3Vh004624@mx.bioceptlabs.com> Content-Type: text/plain; charset="us-ascii" Please unsubscribe me from the list. Jayne Scoggin, Biocept Inc. 858 320 8224 jscoggin@biocept.com ------------------------------ Message: 23 Date: Wed, 2 Aug 2006 12:40:55 -0400 From: "Zajic Kari" Subject: RE: [Histonet] charging and CPT questions To: "Vickroy, Jim" , Message-ID: <095327C7CDBDF64B9E9728A54799091E015CC5A2@ORLEV03.hca.corpad.net> Content-Type: text/plain; charset="iso-8859-1" Hi Jim. I do not do muscle or nerve at my facility but we send to the University of Miami and they charge the following: Muscle 88305 x1: Surgical Path,gross,micro 88313 x2: Paraffin: H&E Plastic 88314 x2: cryostat H&E Trichrome 88319 x8: Enzyme HistoChem: NADH,Esterase, ATPasex3,Cox, Cox & SDH, SDH Nerve 88305 x1: Surg Path,gross,micro 88313 x6: Paraffin H&E,Trichrome,Luxol Fast Blue,Toludine Blue, Crystal Violet,Silver Stain 88314 x2: Cryostat: H&E,Trichrome 88319 x1: Frozen Section Histochem: Esterase 88348 x1: Nerve Electron Micro 88356 x1: Nerve Morphometry 88362 x1: Nerve Teased Preps As far as frozen section touch preps, they came out with new CPT's in 2006 as follows: 88333 Path consult during surgery (cyto exam) initial site ie.touch prep,squash prep physician fee global $75-100+ technical $17-25 88334 cyto exam, EACH ADDITIONAL SITE, touch prep,squash prep physicain fee $40-50+ technical $10-15 this info was given to me by Health Systems Concepts,Inc. hope this helps!!! Kari :) Kari Marie Zajic HT,MLT Histology Supervisor Palms West Hospital Pathology Department 13001 State Road Eighty Loxahatchee, Florida 33470 phone: (561)798-6036 telefax: (561)753-4298 voicemail: (561)753-4299 pager: (561)610-4949 email: Kari.Zajic@HCAHealthcare.com This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Vickroy, Jim Sent: Wednesday, August 02, 2006 12:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] charging and CPT questions Can anyone shed any light on the proper way to code and charge the following: 1. Muscle Biopsy Histochemistry - Frozen sections -88314 or 88319? I'm not sure which one. 2. Touch preps - Done when we do a frozen section. The frozen section is 88331 and the touch prep should be 88333 or 88334? 3. Semi-thin sectioning of nerve biopsies - Plastic embedding In the past we charged all nerve bxs with an 88348 (EM) charge since not only did we do the semi-thin sectioning but we also did the transmission EM. Our neuropathologist now wants the semi-thin sections routinely but not the rest of the EM examination. Thanks Jim Vickroy Technical Supervisor - Surgical and Autopsy Pathology Memorial Medical Center This message (including any attachments) contains confidential information intended for a specific individual and purpose, and is protected by law. If you are not the intended recipient, you should delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 33, Issue 2 *************************************** From Melissa.Gonzalez <@t> cellgenesys.com Wed Aug 2 11:48:36 2006 From: Melissa.Gonzalez <@t> cellgenesys.com (Melissa Gonzalez) Date: Wed Aug 2 12:47:42 2006 Subject: [Histonet] Opinions on cryostats please.. Message-ID: We are looking into budgeting a used/new cryostat for next year, and I was wondering how much other labs have spent on theirs, and how happy they are with the equipment, reliability, service, etc. It's been awhile since I've looked at units and am curious if there are any popular pieces out there right now. Thanks a lot for the input, and feel free to respond off-line. Vendor responses welcome. Melissa Melissa Gonzalez Cell Genesys, Inc. South San Francisco, CA 94080 From brian.chelack <@t> usask.ca Wed Aug 2 12:58:28 2006 From: brian.chelack <@t> usask.ca (Brian Chelack) Date: Wed Aug 2 12:56:15 2006 Subject: [Histonet] Anyone looking for a Ventana Discover? Message-ID: <006d01c6b65d$42304030$0f13e980@PDS04> We have a surplus Ventana Discovery. The project it was originally purchased for failed to materialize and the unit has been sitting unused for the past year or so (It has been used to stain a total of 4100 slides). If anyone has a need for this piece of equipment, give me a call and we can talk about price etc. Brian Chelack Prairie Diagnostic Services 52 Campus Drive Saskatoon, SK S7N 5B4 306-966-7241 From hborgeri <@t> wfubmc.edu Wed Aug 2 13:03:16 2006 From: hborgeri <@t> wfubmc.edu (Hermina Borgerink) Date: Wed Aug 2 13:03:24 2006 Subject: [Histonet] endothelial cell antibody Message-ID: <9AEEF1FB6254224AA355ED285F8491651A8A1BBE@EXCHVS2.medctr.ad.wfubmc.edu> Michele, I have successfully used Novocastra's monoclonal CD34 antibody for endothelial cells on human FFPE tissue. It requires trypsin digestion rather than HIER. Hermina -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Hannah, Michele F. Sent: Wednesday, August 02, 2006 1:26 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] endothelial cell antibody Has anyone worked with an antibody to endothelial cells that has worked well for them? If appropriate, was it successful in lung tissue and/or what were the conditions (FFPE, frozen, ect)? I am looking at several different possibilities and wanted to know what other people were using. Thanks! Michele Michele F. Hannah M.S. Department of Molecular Microbiology & Immunology Johns Hopkins Bloomberg School of Public Health 615 North Wolfe Street Room E3201 Baltimore, Maryland 21205 Phone: (410) 614-7794 Fax: (410) 955-0105 From TJJ <@t> Stowers-Institute.org Wed Aug 2 13:04:46 2006 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Wed Aug 2 13:05:16 2006 Subject: [Histonet] RE:BrdU and GFP staining Message-ID: Steve wrote: >>We have several FFPE blocks from 2003 that express GFP. Our lead scientist would like to perform Brdu IHC in the same slides hoping to express both. As expected the AR removes the GFP green label. Has anyone any experience expressing Brdu and GFP on the same slide. First question I have is - does the fluorescence of the GFP in your tissues survive the paraffin processing? Some labs can demonstrate it, but most can't. I think the secret to success is using a processing temperature that stays at 58 degrees C or below. Low melt point paraffins (of which we have none) are supposed to be superior for post-paraffin processing/embedding direct visualization of GFP fluorescence. In her response, Sarah gave great advice and information, especially regarding the limitation if you expect co-localization. Since the BrdU antibody requires denaturing of some sort, multiple immunostaining techniques (especially fluorescence) can be very tricky. We use the BrdU antibody from Amersham, and the working solution contains DNAse enzyme which may or may not work in a cocktail with another antibody. Our GFP does use HIER (antigen retrieval in citrate buffer) for pretreatment, as does our BrdU. If I were to try this, this would be my ideal approach on mouse tissues: Do single staining first on the tissues using each antibody separately to make sure you get immunostaining using this detection scheme. In parallel, run slides with tissues known to be negative for GFP and BrdU as specificity controls. If all looks good, proceed with double stain protocol. Here's what would probably work in our lab: HIER as usual, for us 10' @95 degreesC in a microwave Block as usual with casein-type blocking reagent, 10' Apply cocktail GFP and BrdU antibodies: rabbit anti-GFP (Novus Biologicals, NB 600-303) at 1:500 + mouse anti-BrdU (Amersham, prepare stock as recommended, but dilute out to 1:50 for working concentration) - incubate 1 hour at room temperature Secondary antibody cocktail - Alexa fluor 488 donkey anti-rabbit 1:300 (GFP label) + Alexa fluor 568 anti-mouse IgG2a 1:300 (BrdU label) - 30 minutes - 1 hour at room temperature DAPI counterstain Run one negative using non-immune serum cocktails for the primary antibody cocktails, everything else the same. Run one slide using above protocol. Verify signal by comparing to your single IHC techniques, and troubleshoot if necessary. Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From malek.j <@t> ghc.org Wed Aug 2 13:53:05 2006 From: malek.j <@t> ghc.org (Malek, Jack M) Date: Wed Aug 2 13:53:12 2006 Subject: [Histonet] EGFR Exon 19 gene mutation Message-ID: <4B2B5C876118AC47998E76D07206505DA0CE0B@ex05.GHCMASTER.GHC.ORG> Does anyone know anything about EGFR as it relates to therapy for Non-Small Cell Lung Cancer? There's an oral abstract from a recent conference that describes 100% response to therapy with the EGFR exon 19 deletion in NSCLC and 75% response with the L858R mutation. Ultimately, I wish to learn if there's anyone offering testing. Jack Malek in Seattle, WA 206-326-3251 Malek.j@ghc.org From rjbuesa <@t> yahoo.com Wed Aug 2 14:24:35 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Aug 2 14:24:39 2006 Subject: [Histonet] endothelial cell antibody In-Reply-To: <1D71A10BB247204A9EFFB9EED3236058019168CE@XCH-VN02.sph.ad.jhsph.edu> Message-ID: <20060802192435.50859.qmail@web61214.mail.yahoo.com> Michele: I used CD34 from Beckton Dickinson monoclonal with HIER at pH6 (citrate) 1:100 dilution with placenta as + control. It always worked superb! Hope this will help you! Ren? J. "Hannah, Michele F." wrote: Has anyone worked with an antibody to endothelial cells that has worked well for them? If appropriate, was it successful in lung tissue and/or what were the conditions (FFPE, frozen, ect)? I am looking at several different possibilities and wanted to know what other people were using. Thanks! Michele Michele F. Hannah M.S. Department of Molecular Microbiology & Immunology Johns Hopkins Bloomberg School of Public Health 615 North Wolfe Street Room E3201 Baltimore, Maryland 21205 Phone: (410) 614-7794 Fax: (410) 955-0105 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Groups are talking. We´re listening. Check out the handy changes to Yahoo! Groups. From weneng2004 <@t> yahoo.com Wed Aug 2 14:53:17 2006 From: weneng2004 <@t> yahoo.com (wen eng) Date: Wed Aug 2 14:53:25 2006 Subject: [Histonet] TUNEL kits Message-ID: <20060802195317.95649.qmail@web53410.mail.yahoo.com> Hello, I am asked to do TUNEL stain on FFPE rodent brain tissue. I found there are many kits are available. Which kits would you recommend? Any help is highly appreciated! Wen --------------------------------- Groups are talking. We´re listening. Check out the handy changes to Yahoo! Groups. From Rcartun <@t> harthosp.org Wed Aug 2 15:22:09 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Aug 2 15:22:40 2006 Subject: [Histonet] EGFR Exon 19 gene mutation In-Reply-To: <4B2B5C876118AC47998E76D07206505DA0CE0B@ex05.GHCMASTER.GHC.ORG> References: <4B2B5C876118AC47998E76D07206505DA0CE0B@ex05.GHCMASTER.GHC.ORG> Message-ID: <44D0D13102000077000013C5@hcnwgwds01.hh.chs> Genzyme (www.genzymegenetics.com) offers EGFR mutation analysis. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Malek, Jack M" 08/02/06 2:53 PM >>> Does anyone know anything about EGFR as it relates to therapy for Non-Small Cell Lung Cancer? There's an oral abstract from a recent conference that describes 100% response to therapy with the EGFR exon 19 deletion in NSCLC and 75% response with the L858R mutation. Ultimately, I wish to learn if there's anyone offering testing. Jack Malek in Seattle, WA 206-326-3251 Malek.j@ghc.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nsdd114 <@t> alltel.net Wed Aug 2 18:26:44 2006 From: nsdd114 <@t> alltel.net (nsdd114@alltel.net) Date: Wed Aug 2 18:26:48 2006 Subject: [Histonet] Mohs Tech salaries Message-ID: <20060802232644.FGSK28894.ispmxmta05-srv.windstream.net@webmail-relay.alltel.net> I was wondering if those of you who are doing Mohs would respond to my question. I am HT-ASCP certified and have 15 years in the field. I am currently doing Mohs, because the hours and the pay are better in Mohs in my area. I like doing Mohs and we keep very busy. We started the lab a year ago and have one Mohs surgeon and are currently doing about 1500 cases a year. Of course that is cases not blocks, because we frequently do cases with anywhere from 6-25 pieces. Our practice would like to expand our service and we have tried three techs, all on the job trained and not certified. I personally know techs who were OJT trained who are top notch techs. Two of the techs we interviewed weren't sufficiently skilled to work really independently at high volume and trouble shoot routines. One didn't know how to tell if she had a complete skin edge under the scope and she had 3 years of experience. That's scarey. The 3rd tech is pretty capable, but is also a CMA and prefers the nursing position. My question is "Does anyone know of a Mohs salary survey or any guidelines as far as pay goes for both certified and non-certified techs?" I personally don't care if someone is confident and capable whether they are certified or not since it isn't required at this time, but we do want to establish a pay scale that rewards people based on their overall training and skill. I do suspect that the next few years will bring some kind of standardized training requirements and/or certification for Mohs techs. If anyone has any input I'd appreciate it. We are in the south central east coast area. Thanks, Nancy > > From: histonet-request@lists.utsouthwestern.edu > Date: 2006/08/02 Wed PM 12:01:20 CDT > To: histonet@lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 33, Issue 2 > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: Foxp3 on Murine tissue (Melissa Gonzalez) > 2. RE:BrdU and GFP staining (Pixley, Sarah (pixleysk)) > 3. Staining cultured cells (Pixley, Sarah (pixleysk)) > 4. immunohistochemical analysis for connective tissue (Xilong Li) > 5. ImmunoVision Technologies, Co. (Helen E Johnson) > 6. Re: ImmunoVision Technologies, Co. (Ron Lagerquist) > 7. Pennsylvania Histology Meeting for October 2006 (Pamela Marcum) > 8. RE: Ethanol/other fixations for frozen sections (Tony Henwood) > 9. Looking for Florida histotechs--or people who are interested > in Florida relocation: we help with the license. (Cheryl) > 10. article from J of histotechnology (louise renton) > 11. Zinc-Based Fixative (Shirley PHUA) > 12. prefilled formalin containers (Vickroy, Jim) > 13. Re: prefilled formalin containers (Jackie M O'Connor) > 14. '07 TSH Meeting (Ford Royer) > 15. The New VIP (Santana, Diane) > 16. Re: The New VIP (Fred Underwood) > 17. RE: The New VIP. . (Henry, Charlene) > 18. Re: The New VIP (crains@wpmpath.com) > 19. prefilled formalin containers (Stahl, Michael) > 20. Re: The New VIP (Andrea Grantham) > 21. charging and CPT questions (Vickroy, Jim) > 22. please unsubscribe me from the list (Jayne Scoggin) > 23. RE: charging and CPT questions (Zajic Kari) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 1 Aug 2006 10:18:52 -0700 > From: "Melissa Gonzalez" > Subject: [Histonet] RE: Foxp3 on Murine tissue > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > I've also tried several of the products out there with no success on > murine tissues as well. I would be interested in hearing if anyone has > had success with FoxP3 yet on murine tissues, and what you could use as > a positive control. > > Thanks, > Melissa > ------------------------------ > > Message: 7 > Date: Mon, 31 Jul 2006 14:02:03 -0500 > From: "Drew Allan Roenneburg" > Subject: [Histonet] Foxp3 on Murine tissue > To: > Message-ID: <44CE0D5B0200009C00001F23@gw.surgery.wisc.edu> > Content-Type: text/plain; charset=US-ASCII > > Hi, I was wondering if anyone has stained for foxp3 on frozen or FFPE > mouse tissues (spleen). I have tried the Abcam rabbit anti-human foxp3 > antibody that works on FFPE human and monkey (tonsil and spleen), > however have had little success on mouse. Thanks > Drew Roenneburg > > > > ------------------------------ > > Message: 2 > Date: Tue, 1 Aug 2006 13:45:30 -0400 > From: "Pixley, Sarah \(pixleysk\)" > Subject: [Histonet] RE:BrdU and GFP staining > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Dear Steve: > I would stain for GFP with an antibody to GFP and do labeling with DAB. > I recommend the Elite ABC kits. The DAB is moderately resistant to the > BrdU penetration protocols. Then, fix your tissues well with > paraformaldehyde, i.e. 15 mins, 4%, and proceed with the BrdU, using a > system that labels with a fluorescent antibody. This will only work, > however, if your GFP is in different cells, or it is in the cytoplasm of > large cells that have no nuclear staining. The DAB can obscure the > secondary fluorescent labeling. > > Good luck. Stay Cool back at you. > Sarah Pixley, > Ohio > > Message: 2 > Date: Mon, 31 Jul 2006 10:22:41 -0700 (PDT) > From: Steven Coakley > Subject: [Histonet] BrdU and GFP expression > To: Histonet@lists.utsouthwestern.edu > Message-ID: <20060731172241.65699.qmail@web38215.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Good afternoon from WI, > > We have several FFPE blocks from 2003 that express GFP. Our lead > scientist would like to perform Brdu IHC in the same slides hoping to > express both. As expected the AR removes the GFP green label. Has > anyone any experience expressing Brdu and GFP on the same slide. > > Stay cool, > > Steve > > > > > > ------------------------------ > > Message: 3 > Date: Tue, 1 Aug 2006 13:49:31 -0400 > From: "Pixley, Sarah \(pixleysk\)" > Subject: [Histonet] Staining cultured cells > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > > To stain cultured cells we routinely fix with 4% paraformaldehyde for > 15-20 mins. But if the antigen is sensitive to aldehydes, then we fix > with ice-cold 100% methanol for 5-10 mins. Your acetone technique for 30 > mins is extremely harsh for cultured cells. If you want to continue with > it, I would fix with the acetone for only a few minutes and try that. > > As for the other person who asked about staining cultured cells, we do > exactly the same ICC as on sections, although we usually are able to > dilute the primary antibodies more than with frozen tissue sections > (using paraformaldehyde perfused tissues). > > Sarah Pixley > Ohio > > > Message: 4 > Date: Mon, 31 Jul 2006 13:41:54 -0400 > From: Christopher C Overend > Subject: [Histonet] IFA staining problems > To: histonet@lists.utsouthwestern.edu > Message-ID: <1fbaf901fb5b0e.1fb5b0e1fbaf90@huskymail.uconn.edu> > Content-Type: text/plain; charset=us-ascii > > I am new to the technique of immunofluorescence, and have been doing > some work with it lately on cultured cells. Specifically, I have been > staining intracellular virus with very good results. However, when i try > to detect cellular proteins, I do not get any staining. The proceedure i > have been using consists of "fixation" with 90% acetone for 30 min, at > 4degrees C. the rest of the process had been much like an ELISA, all > incubations have been for 30 min at 4degrees. The buffer i have been > using is PBS, 1%BSA, 0.1%NaN3. Someone mentioned the problem could be > from the acetone and suggested trying a glyoxal fixative. Can anyone > offer some insights, or if there is a different protocol i might have to > follow using a glyoxal fixative with tissue culture? > Thank you! > Chris > > Christopher Overend > Ph.D Student > University of Connecticut > Department of Pathobiology and Veterinary Sciences Storrs, CT > Christopher.overend@uconn.edu > > > > > > ------------------------------ > > Message: 4 > Date: Tue, 01 Aug 2006 13:23:06 -0500 > From: "Xilong Li" > Subject: [Histonet] immunohistochemical analysis for connective tissue > To: > Message-ID: <44CF55BA020000F000004E06@swnw124.swmed.edu> > Content-Type: text/plain; charset=US-ASCII > > Hi, All members, > > I am looking for a consistent protocol to stain collagen of connective > tissues in muscle frozen sections by immunohistochemical analysis > including information for primary antibody and second antibody used in > the protocols. > > Thanks in advance. > > xilong li > > Dr. Xilong Li > Hypertension Division, Internal Medicine > University of Texas Southwestern Medical Center > 5323 Harry Hiness Blvd-J4.142 > Dallas, TX 75390 > > Tel: 214-648-9966(L) > Fax: 214-648-7902 > > > > > ------------------------------ > > Message: 5 > Date: Tue, 1 Aug 2006 14:24:37 -0400 > From: Helen E Johnson > Subject: [Histonet] ImmunoVision Technologies, Co. > To: histonet@lists.utsouthwestern.edu > Message-ID: > > > Content-Type: text/plain; charset=US-ASCII > > > Hi Histonetters, > Does anyone know if ImmunoVision Technologies, Co. is still in > business? No one answers the phone ( 650-992-7563). > Helen > (hej01@health.state.ny.us) > > > > > ------------------------------ > > Message: 6 > Date: Tue, 1 Aug 2006 11:32:41 -0700 (PDT) > From: Ron Lagerquist > Subject: Re: [Histonet] ImmunoVision Technologies, Co. > To: Helen E Johnson , > histonet@lists.utsouthwestern.edu > Message-ID: <20060801183241.80617.qmail@web56106.mail.re3.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > They were purchased by VisionBiosystems (800-753-7264) > http://www.vision-bio.com/media_releases.html > > Ron > > Helen E Johnson wrote: > > Hi Histonetters, > Does anyone know if ImmunoVision Technologies, Co. is still in > business? No one answers the phone ( 650-992-7563). > Helen > (hej01@health.state.ny.us) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Do you Yahoo!? > Next-gen email? Have it all with the all-new Yahoo! Mail Beta. > > ------------------------------ > > Message: 7 > Date: Tue, 01 Aug 2006 14:33:51 -0400 > From: Pamela Marcum > Subject: [Histonet] Pennsylvania Histology Meeting for October 2006 > To: histonet@lists.utsouthwestern.edu > Message-ID: <6.1.1.1.2.20060801142646.01a26d88@mail.vet.upenn.edu> > Content-Type: text/plain; charset="us-ascii"; format=flowed > > > > Good Afternoon All, > > We have our web site up and partially up. We do have our program online > and have the program in the download file area for you to review and if > register. The program can be printed off. Just go to www.pahisto.org and > look for downloads in the top line. Click on and open or save. The format > is a Microsoft Word document. If you have a problem or would need a > pre-printing copy please let either me or Gloria Limetti know. Gloria can > be reached at glorialimetti@yahoo.com or 412-647-8535. > > Thanks for your patience and we will be updating the program with vendors > and other information as we near the meeting in October. > > > Best Regards, > > Pamela A Marcum > Manager, Histology Special Procedures > University of Pennsylvania > School of Veterinary Medicine > R.S. Reynolds Jr. CORL > New Bolton Center > 382 West Street Road > Kennett Square, PA 19348 > > Phone - 610-925-6278 > Fax - 610-925-8120 > E-mail - pmarcum@vet.upenn.edu > > > > > > ------------------------------ > > Message: 8 > Date: Wed, 2 Aug 2006 10:49:46 +1000 > From: "Tony Henwood" > Subject: RE: [Histonet] Ethanol/other fixations for frozen sections > To: "Andrea T. Hooper" , "Histonet" > > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Urgent Frozen Sections for H&E - we fix in methanol > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) > Laboratory Manager & Senior Scientist > The Children's Hospital at Westmead, > Locked Bag 4001, Westmead, 2145, AUSTRALIA. > Tel: 612 9845 3306 > Fax: 612 9845 3318 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea > T. Hooper > Sent: Monday, 31 July 2006 10:45 PM > To: Histonet > Subject: [Histonet] Ethanol/other fixations for frozen sections > > > Just taking a survey .... What are everyone's thoughts on using > ethanol for frozen sections? I have a colleague who swears by it but > I am an acetone person myself. > > Thoughts, comments, input on what is the best fix for frozen section > IHC? > > Thanks! > -- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************** > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead > > This note also confirms that this email message has been > virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************** > > > > > ------------------------------ > > Message: 9 > Date: Tue, 1 Aug 2006 19:40:34 -0700 (PDT) > From: Cheryl > Subject: [Histonet] Looking for Florida histotechs--or people who are > interested in Florida relocation: we help with the license. > To: Histonet > Message-ID: <20060802024034.14424.qmail@web50904.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hello everyone-- > > Florida's registry process has created a major shortage of qualified techs in the state. We have a number of Florida permanent and temp opportunities. The permanent labs will consider transitional job situations to allow you to work while your license is processed. We also have a number of temp situations for a great pay scale for techs registered to work in the state. Travel isn't for everyone, but these are good labs and a great way to start. > > Either way, we'll help you through the process to get you through it as quickly as possible. If you're even curious, give us a call. Of course we have a number of other opportunities including sales and management all over the country. We won't work with every lab--those we place find themselves in jobs that fit--where they are happy to go to work. We're here to help and no fee to you. > > Tiffany is in the office and I'm traveling as a temp for a couple of weeks--as always, referral bonuses are available--share us with your friends! > > Tiffany/Office 281.852.9457 > Cheryl/Cell 281.883.7704 > or this number follows me around: 800.756.3309 (it will ring a long time to find me--please be patient and leave your phone number!) > > We return all calls. > > Thanks! > > Cheryl > > > > Cheryl Kerry, HT(ASCP) > Full Staff Inc. > Staffing the AP Lab by helping one Tech at a time. > 281.883.7704 c > 281.852.9457 o > admin@fullstaff.org > > Sign up for the FREE monthly newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. > > ------------------------------ > > Message: 10 > Date: Wed, 2 Aug 2006 09:47:06 +0200 > From: "louise renton" > Subject: [Histonet] article from J of histotechnology > To: Histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Hi can anyone share a copy of this article? I would be very grateful > > "Entering the realm of mineralized bone processing: a review of the > literature and techniques." J Histotechnol 1997:20 (3) p259-266 > > Many thanks > > Louise Renton > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > "There are nights when the wolves are silent and only the moon howls". > George Carlin > No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > > > > ------------------------------ > > Message: 11 > Date: Wed, 2 Aug 2006 16:19:02 +0800 > From: Shirley PHUA > Subject: [Histonet] Zinc-Based Fixative > To: histonet@lists.utsouthwestern.edu > Message-ID: > > > Content-Type: text/plain; charset="US-ASCII" > > Dear All, > > Reference Article: > Lab Invest 2003 Jun;83(6):889-99 > Zinc-based fixative improves preservation of genomic DNA and proteins in > histoprocessing of human tissues > > Anyone out there know of the exact composition of any zinc-based fixative > that is based on the following? > 1. 0.5% Zinc Chloride and > 2. 0.5% Zinc Acetate in 0.1M Tris base buffer containing 0.05% calcium > acetate > > > Many thanks & regards, > Shirley Phua > Histopathology Laboratory > Centre for Forensic Medicine > Health Sciences Authority > Singapore > > > > ------------------------------ > > Message: 12 > Date: Wed, 2 Aug 2006 08:23:30 -0500 > From: "Vickroy, Jim" > Subject: [Histonet] prefilled formalin containers > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > > > We have experienced problems with prefilled formalin containers that we > send to our clients. We have tried two different vendors and they both > leak. One of the container types claims to have an o-ring type lid and > the other does not have an o-ring. The containers with the o-ring seems > to leak as much as the others. In fact the o-ring type sometimes leak > when they are received from the vendor. If anyone has dealt with this > successfully please let me know how you solved it. > > > > Jim Vickroy > > Technical Supervisor - Surgical and Autopsy Pathology > > Memorial Medical Center > > > > > > This message (including any attachments) contains confidential information intended for a > specific individual and purpose, and is protected by law. If you are not the intended recipient, > you should delete this message. Any disclosure, copying, or distribution of this message, or the > taking of any action based on it, is strictly prohibited. > > > ------------------------------ > > Message: 13 > Date: Wed, 2 Aug 2006 08:41:32 -0500 > From: "Jackie M O'Connor" > Subject: Re: [Histonet] prefilled formalin containers > To: "Vickroy, Jim" > Cc: histonet@lists.utsouthwestern.edu, > histonet-bounces@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > Are they leaking on the return trip? Hard to get your clients to make > sure they are screwed on properly. Each vial/jar should be contained > within a secondary leakproof bag, anyway - to prevent leaks in transit. > Give Surgipath a call. > > Jackie O' > > > > "Vickroy, Jim" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 08/02/2006 08:23 AM > > To > > cc > > Subject > [Histonet] prefilled formalin containers > > > > > > > > > We have experienced problems with prefilled formalin containers that we > send to our clients. We have tried two different vendors and they both > leak. One of the container types claims to have an o-ring type lid and > the other does not have an o-ring. The containers with the o-ring seems > to leak as much as the others. In fact the o-ring type sometimes leak > when they are received from the vendor. If anyone has dealt with this > successfully please let me know how you solved it. > > > > Jim Vickroy > > Technical Supervisor - Surgical and Autopsy Pathology > > Memorial Medical Center > > > > > > This message (including any attachments) contains confidential information > intended for a > specific individual and purpose, and is protected by law. If you are not > the intended recipient, > you should delete this message. Any disclosure, copying, or distribution > of this message, or the > taking of any action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 14 > Date: Wed, 2 Aug 2006 08:42:33 -0500 > From: "Ford Royer" > Subject: [Histonet] '07 TSH Meeting > To: > Message-ID: <003101c6b639$82553090$7701a80a@Ford> > Content-Type: text/plain; charset="us-ascii" > > Would the person(s) who are organizing next years Texas Society of > Histotechnology meeting please contact me? > > Thanks! > > ~ Ford > > Ford M. Royer, MT(ASCP) > Histology Product Manager > Minnesota Medical, Inc. > 7177 Madison Ave. W. > Golden Valley, MN 55427-3601 > CELL: 612-839-1046 > Phone: 763-542-8725 > Fax: 763-546-4830 > eMail: froyer@bitstream.net > > > > > ------------------------------ > > Message: 15 > Date: Wed, 2 Aug 2006 10:05:40 -0400 > From: "Santana, Diane" > Subject: [Histonet] The New VIP > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: <4C96AA62BADFD81180CB0002A5AD67FB07113AC5@MAILPMA> > Content-Type: text/plain; charset="iso-8859-1" > > I have a question and please nobody get all defensive about this, but has > anybody recently bought the VIP 5 tissue processor? If you have, do or did > you have any problems with it? > Thanks > Diane Santana > PMA > Haverhill, Mass. > > > > ------------------------------ > > Message: 16 > Date: Wed, 02 Aug 2006 10:31:26 -0400 > From: "Fred Underwood" > Subject: Re: [Histonet] The New VIP > To: , > Message-ID: > Content-Type: text/plain; charset=US-ASCII > > I've had mine for a year and (knock, knock on wood) have had no > problems. > > Fred > > >>> "Santana, Diane" 08/02 10:05 AM >>> > I have a question and please nobody get all defensive about this, but > has > anybody recently bought the VIP 5 tissue processor? If you have, do or > did > you have any problems with it? > Thanks > Diane Santana > PMA > Haverhill, Mass. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 17 > Date: Wed, 2 Aug 2006 09:36:48 -0500 > From: "Henry, Charlene" > Subject: RE: [Histonet] The New VIP. . > To: "Santana, Diane" , > > Message-ID: > <5CB39BCA5724F349BCB748675C6CA1A2099A1D6A@SJMEMXMB02.stjude.sjcrh.local> > > Content-Type: text/plain; charset="US-ASCII" > > We purchased a VIP5 a few years ago and had several problems so it was > replaced with a new VIP5. Since that time we have had no problems and it > has been a reliable instrument. > Charlene > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Santana, > Diane > Sent: Wednesday, August 02, 2006 9:06 AM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] The New VIP. . > > I have a question and please nobody get all defensive about this, but > has anybody recently bought the VIP 5 tissue processor? If you have, do > or did you have any problems with it? > Thanks > Diane Santana > PMA > Haverhill, Mass. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 18 > Date: Wed, 2 Aug 2006 10:30:16 -0500 > From: > Subject: Re: [Histonet] The New VIP > To: "Santana, Diane" , > "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > <20060802153018.PMMJ26069.dukecmmtao03.coxmail.com@dukecmmtao03> > Content-Type: text/plain; charset=ISO-8859-1 > > We have a VIP 5 that we purchased a little over a year ago. Have not had a single problem with it so far. > > Chris Rains > WPM Pathology Laboratory > Salina, KS > > > > From: "Santana, Diane" > > Date: 2006/08/02 Wed AM 09:05:40 CDT > > To: "'histonet@lists.utsouthwestern.edu'" > > Subject: [Histonet] The New VIP > > > > I have a question and please nobody get all defensive about this, but has > > anybody recently bought the VIP 5 tissue processor? If you have, do or did > > you have any problems with it? > > Thanks > > Diane Santana > > PMA > > Haverhill, Mass. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > Important: This email and any attachments may contain confidential information subject to protection under the Federal Standards for Privacy of Individually Identifiable Health Information (45 C.F.R. Parts 160 and 164). If you or your organization is a "Covered Entity" under the above mentioned regulations, you are obligated to treat such information in a manner consistent with the regulations. If it appears that this email was sent to you in error, (1) you are prohibited from utilizing or disseminating this email or any attachments; (2) please immediately delete it from your computer and any servers or other locations where it might be stored and email (crains@wpmpath.com) or call (Chris Rains) at 785-823-7201 advising that you have done so. We appreciate your cooperation. > > > > > ------------------------------ > > Message: 19 > Date: Wed, 2 Aug 2006 11:42:17 -0400 > From: "Stahl, Michael" > Subject: [Histonet] prefilled formalin containers > To: > Message-ID: > <4C878E714B21EB4F8EB159777B8822EE5B6834@bvfyms01.net.bvha.org> > Content-Type: text/plain; charset="us-ascii" > > We use a Surgipath (cat #008100 which are the pre-filled 60ml jars. We > found these to be almost leak proof. However, they do leak when the jars > arnt screwed on correctly. But we agree, its not pleasant to have a > specimen jar in a bio hazard bag full of fixative. Just try to find that > cervial biospy! > > Michael P. Stahl HT (ASCP) > BVRHC > 145 W Wallace St > Findlay, OH 45840 > mstahl@bhva.org > > > ------------------------------ > > Message: 20 > Date: Wed, 02 Aug 2006 08:55:45 -0700 > From: Andrea Grantham > Subject: Re: [Histonet] The New VIP > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > <4.3.2.7.2.20060802085142.00cec460@algranth.inbox.email.arizona.edu> > Content-Type: text/plain; charset="us-ascii"; format=flowed > > I just got a new VIP 5 and I love it. Wanted one all my (histotech) life > and I'm so happy to finally have it! It has been great. So easy to use and > the tissues are coming out wonderful. Knock-on-wood it will do as well as > the old Tissue Tek dipper/dunker that it is replacing. > Andi > > > At 10:30 AM 8/2/2006 -0500, crains@wpmpath.com wrote: > >We have a VIP 5 that we purchased a little over a year ago. Have not had > >a single problem with it so far. > > > >Chris Rains > >WPM Pathology Laboratory > >Salina, KS > > > > > > From: "Santana, Diane" > > > Date: 2006/08/02 Wed AM 09:05:40 CDT > > > To: "'histonet@lists.utsouthwestern.edu'" > > > > > Subject: [Histonet] The New VIP > > > > > > I have a question and please nobody get all defensive about this, but has > > > anybody recently bought the VIP 5 tissue processor? If you have, do or did > > > you have any problems with it? > > > Thanks > > > Diane Santana > > > PMA > > > Haverhill, Mass. > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > >Important: This email and any attachments may contain confidential > >information subject to protection under the Federal Standards for Privacy > >of Individually Identifiable Health Information (45 C.F.R. Parts 160 and > >164). If you or your organization is a "Covered Entity" under the above > >mentioned regulations, you are obligated to treat such information in a > >manner consistent with the regulations. If it appears that this email was > >sent to you in error, (1) you are prohibited from utilizing or > >disseminating this email or any attachments; (2) please immediately delete > >it from your computer and any servers or other locations where it might be > >stored and email (crains@wpmpath.com) or call (Chris Rains) at > >785-823-7201 advising that you have done so. We appreciate your cooperation. > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ..................................................................... > : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : > : Sr. Research Specialist University of Arizona : > : (office: AHSC 4212) P.O. Box 245044 : > : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : > : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : > :...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > > > > ------------------------------ > > Message: 21 > Date: Wed, 2 Aug 2006 11:08:00 -0500 > From: "Vickroy, Jim" > Subject: [Histonet] charging and CPT questions > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > > > Can anyone shed any light on the proper way to code and charge the > following: > > > > 1. Muscle Biopsy Histochemistry - Frozen sections -88314 or 88319? > I'm not sure which one. > > > > 2. Touch preps - Done when we do a frozen section. The frozen > section is 88331 and the touch prep should be 88333 or 88334? > > > > 3. Semi-thin sectioning of nerve biopsies - Plastic embedding > In the past we charged all nerve bxs with an 88348 (EM) charge since > > not only did we do the semi-thin sectioning but we > also did the transmission EM. Our neuropathologist now wants the > semi-thin sections > > routinely but not the rest of the EM examination. > > > > Thanks > > > > Jim Vickroy > > Technical Supervisor - Surgical and Autopsy Pathology > > Memorial Medical Center > > > > > > This message (including any attachments) contains confidential information intended for a > specific individual and purpose, and is protected by law. If you are not the intended recipient, > you should delete this message. Any disclosure, copying, or distribution of this message, or the > taking of any action based on it, is strictly prohibited. > > > ------------------------------ > > Message: 22 > Date: Wed, 2 Aug 2006 09:45:21 -0700 > From: "Jayne Scoggin" > Subject: [Histonet] please unsubscribe me from the list > To: > Message-ID: <200608021624.k72GO3Vh004624@mx.bioceptlabs.com> > Content-Type: text/plain; charset="us-ascii" > > Please unsubscribe me from the list. > > Jayne Scoggin, Biocept Inc. > 858 320 8224 > jscoggin@biocept.com > > > > > > ------------------------------ > > Message: 23 > Date: Wed, 2 Aug 2006 12:40:55 -0400 > From: "Zajic Kari" > Subject: RE: [Histonet] charging and CPT questions > To: "Vickroy, Jim" , > > Message-ID: > <095327C7CDBDF64B9E9728A54799091E015CC5A2@ORLEV03.hca.corpad.net> > Content-Type: text/plain; charset="iso-8859-1" > > Hi Jim. I do not do muscle or nerve at my facility but we send to the University of Miami and they charge the following: > > Muscle > 88305 x1: Surgical Path,gross,micro > 88313 x2: Paraffin: H&E Plastic > 88314 x2: cryostat H&E Trichrome > 88319 x8: Enzyme HistoChem: NADH,Esterase, ATPasex3,Cox, Cox & SDH, SDH > > Nerve > 88305 x1: Surg Path,gross,micro > 88313 x6: Paraffin H&E,Trichrome,Luxol Fast Blue,Toludine Blue, Crystal Violet,Silver Stain > 88314 x2: Cryostat: H&E,Trichrome > 88319 x1: Frozen Section Histochem: Esterase > 88348 x1: Nerve Electron Micro > 88356 x1: Nerve Morphometry > 88362 x1: Nerve Teased Preps > > As far as frozen section touch preps, they came out with new CPT's in 2006 as follows: > 88333 Path consult during surgery (cyto exam) initial site ie.touch prep,squash prep > physician fee global $75-100+ > technical $17-25 > 88334 cyto exam, EACH ADDITIONAL SITE, touch prep,squash prep > physicain fee $40-50+ > technical $10-15 > this info was given to me by Health Systems Concepts,Inc. > > hope this helps!!! > Kari :) > > Kari Marie Zajic HT,MLT > Histology Supervisor > Palms West Hospital > Pathology Department > 13001 State Road Eighty > Loxahatchee, Florida 33470 > phone: (561)798-6036 > telefax: (561)753-4298 > voicemail: (561)753-4299 > pager: (561)610-4949 > email: Kari.Zajic@HCAHealthcare.com > > This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL > information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Vickroy, > Jim > Sent: Wednesday, August 02, 2006 12:08 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] charging and CPT questions > > > > > Can anyone shed any light on the proper way to code and charge the > following: > > > > 1. Muscle Biopsy Histochemistry - Frozen sections -88314 or 88319? > I'm not sure which one. > > > > 2. Touch preps - Done when we do a frozen section. The frozen > section is 88331 and the touch prep should be 88333 or 88334? > > > > 3. Semi-thin sectioning of nerve biopsies - Plastic embedding > In the past we charged all nerve bxs with an 88348 (EM) charge since > > not only did we do the semi-thin sectioning but we > also did the transmission EM. Our neuropathologist now wants the > semi-thin sections > > routinely but not the rest of the EM examination. > > > > Thanks > > > > Jim Vickroy > > Technical Supervisor - Surgical and Autopsy Pathology > > Memorial Medical Center > > > > > > This message (including any attachments) contains confidential information intended for a > specific individual and purpose, and is protected by law. If you are not the intended recipient, > you should delete this message. Any disclosure, copying, or distribution of this message, or the > taking of any action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 33, Issue 2 > *************************************** > From dharclerode <@t> cytoritx.com Wed Aug 2 20:52:42 2006 From: dharclerode <@t> cytoritx.com (Donna Harclerode) Date: Wed Aug 2 20:51:21 2006 Subject: [Histonet] Endothelial Cell Markers and Cell Staining Message-ID: <3DE0F644E093DF4BAE80C254176696A5131E69@mp-mailserver.macropore.com> Hi Hannah I do lots of human endothelial staining and my best all around general endothelial marker is CD 31 from PharMingen, clone WM59 cat # 550389. I use it in frozen sections (1:40 with fluorescent secondary or Labeled step avidin biotin and DAB)fixed for 5 minutes in 75% acetone 25% ethanol (Thanks for this fix recommendation years ago Gayle Callas!) or in cells fixed 15 minutes in 4%PFA. I usually incubate primaries overnight at 4oC, but also sometimes do 1-2 hours on the counter with rocking. My other endothelial markers are also from PharMingen and some work in paraffin (sort of) but work great in frozen and cells. Human CD34, CD105, CD106 and CD144 are all very nice markers for different types of endothelial cells with the same fixation in both cells and frozen sections. PharMingen makes excellent endothelial markers for mouse and rat too. The Rat CD 31 from PharMingen cross reacts in pig if anyone cares. I have been staining lots of tissue culture plates and chamber slides with these markers double labeled with smooth muscle myosin or vWF and DAPI nuclei. I do not like the 12 well plates and much prefer the 24. For some reason the 12 well plates that we have are hydrophobic and I have problems covering the cells. In a 24 well plate I only use about 100ul per well and it works nicely I would not recommend most of PharMingen endothelial markers for paraffin sections. Christopher I noticed a couple things that might help your staining. I would not use sodium azide in an antibody diluent for staining- the amount may not hurt, but it sure does not help. I use azide in stock antibody if they do not come in with azide, but would not add more when making up for a IHC (or ICC, if you prefer staining solution) I also do not use BSA anymore- I have found my best results are when I use 2-5% of whatever normal serum from species your secondary is made in and not use BSA. You also have no surfactant of any type to help get through the cell membrane. This last one would be my first guess as the problem. There are also antibodies that do not work well in cells and they work great in paraffin sections- I have found a couple that I have rejected for cells and frozen that are great in paraffin sections. So much depends on your antibody- they do not all cooperate in all formats I want them to work in. You can make your own diluent with Tween or Triton X 100 but I highly recommend the prepared diluents. I think there may be some casein in them, but do not know the secret formula. I use Dako diluent cat S0809 for all my primary and secondary dilutions. (I add 2% normal donkey serum in the primary only because all my secondaries are made in donkey) My isotype specific secondaries are the only exception - they are all made in goat so I would use normal goat if I am doing multi color all mouse, but different isotype abs. I used Dako diluent when optimizing the PharMingen antibodies years ago (I set up the original IHC testing and QC specs for all PharMingen antibodies) - I would expect other companies' diluent to work well. One thing I have noticed is in antibodies that specifically say to "use no Triton in your antibody diluent" - the Dako diluent still works great. I have complete penetration of cells and do not damage any of the sensitive markers for special antibodies. I have never found a primary antibody that works better in another homemade diluent other than Dako. I have a protocol for plates or chamber slides I can email it if you want it. Good Luck Donna Harclerode, HT, (ASCP), HTL, QIHC Scientist / Immunohistochemistry Cytori Therapeutics 3020 Callan Rd. San Diego, CA 92121 858-458-0900 ext 5416 fax 858 200-0945 dharclerode@cytoritx.com From Dmborel <@t> aol.com Wed Aug 2 21:09:06 2006 From: Dmborel <@t> aol.com (Dmborel@aol.com) Date: Wed Aug 2 21:09:29 2006 Subject: [Histonet] Position open for Histotech/Grossing Tech Topeka, Kansas Message-ID: We have an opening for a full time position Monday thru Friday day shift for a tech experienced in routine histology and gross examination of skin biopsies. The position also involves considerable clerical work in the billing and transcription area of the business as well as some client services and marketing activities. We will train you in gross exam techniques if needed. We are a CLIA certified non hospital based private laboratory serving physicians' offices in Kansas and the greater Kansas City area, with a primary emphasis on Dermatopathology and a secondary emphasis on ThinPrep Cytopathology. Please contact Jan Rice, office manager Pathology Services, PA/Dermatopathology Diagnostics 5650 SW 29th Street Topeka, Kansas 66614-2443 Telephone and Fax (785) 272-4783 email _jrice@pspa.kscoxmail.com_ (mailto:jrice@pspa.kscoxmail.com) _www.dermpathdx.com_ (http://www.dermpathdx.com) From lpwenk <@t> sbcglobal.net Wed Aug 2 21:17:22 2006 From: lpwenk <@t> sbcglobal.net (Lee & Peggy Wenk) Date: Wed Aug 2 21:18:23 2006 Subject: [Histonet] Mohs Tech salaries In-Reply-To: <20060802232644.FGSK28894.ispmxmta05-srv.windstream.net@webmail-relay.alltel.net> Message-ID: <001f01c6b6a2$f461dfd0$6ca3ff44@HPPav2> Not specifically for Mohs, but I thought I would let Histonetters know that the 2005 ASCP Wage and Vacancy survey results are now on the ASCP BOR webpage: http://www.ascp.org/Certification/ForProgramDirectors/research/default.aspx Click on 2005. Survey done Nov. 2005, will be published in the August 2006 "Laboratory Medicine". Found it on their website last week. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of nsdd114@alltel.net Sent: Wednesday, August 02, 2006 7:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mohs Tech salaries I was wondering if those of you who are doing Mohs would respond to my question. I am HT-ASCP certified and have 15 years in the field. I am currently doing Mohs, because the hours and the pay are better in Mohs in my area. I like doing Mohs and we keep very busy. We started the lab a year ago and have one Mohs surgeon and are currently doing about 1500 cases a year. Of course that is cases not blocks, because we frequently do cases with anywhere from 6-25 pieces. Our practice would like to expand our service and we have tried three techs, all on the job trained and not certified. I personally know techs who were OJT trained who are top notch techs. Two of the techs we interviewed weren't sufficiently skilled to work really independently at high volume and trouble shoot routines. One didn't know how to tell if she had a complete skin edge under the scope and she had 3 years of experience. That's scarey. The 3rd tech is pretty capable, but is also a CMA and prefers the nursing position. My question is "Does anyone know of a Mohs salary survey or any guidelines as far as pay goes for both certified and non-certified techs?" I personally don't care if someone is confident and capable whether they are certified or not since it isn't required at this time, but we do want to establish a pay scale that rewards people based on their overall training and skill. I do suspect that the next few years will bring some kind of standardized training requirements and/or certification for Mohs techs. If anyone has any input I'd appreciate it. We are in the south central east coast area. Thanks, Nancy > > From: histonet-request@lists.utsouthwestern.edu > Date: 2006/08/02 Wed PM 12:01:20 CDT > To: histonet@lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 33, Issue 2 > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: Foxp3 on Murine tissue (Melissa Gonzalez) > 2. RE:BrdU and GFP staining (Pixley, Sarah (pixleysk)) > 3. Staining cultured cells (Pixley, Sarah (pixleysk)) > 4. immunohistochemical analysis for connective tissue (Xilong Li) > 5. ImmunoVision Technologies, Co. (Helen E Johnson) > 6. Re: ImmunoVision Technologies, Co. (Ron Lagerquist) > 7. Pennsylvania Histology Meeting for October 2006 (Pamela Marcum) > 8. RE: Ethanol/other fixations for frozen sections (Tony Henwood) > 9. Looking for Florida histotechs--or people who are interested > in Florida relocation: we help with the license. (Cheryl) > 10. article from J of histotechnology (louise renton) > 11. Zinc-Based Fixative (Shirley PHUA) > 12. prefilled formalin containers (Vickroy, Jim) > 13. Re: prefilled formalin containers (Jackie M O'Connor) > 14. '07 TSH Meeting (Ford Royer) > 15. The New VIP (Santana, Diane) > 16. Re: The New VIP (Fred Underwood) > 17. RE: The New VIP. . (Henry, Charlene) > 18. Re: The New VIP (crains@wpmpath.com) > 19. prefilled formalin containers (Stahl, Michael) > 20. Re: The New VIP (Andrea Grantham) > 21. charging and CPT questions (Vickroy, Jim) > 22. please unsubscribe me from the list (Jayne Scoggin) > 23. RE: charging and CPT questions (Zajic Kari) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 1 Aug 2006 10:18:52 -0700 > From: "Melissa Gonzalez" > Subject: [Histonet] RE: Foxp3 on Murine tissue > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > I've also tried several of the products out there with no success on > murine tissues as well. I would be interested in hearing if anyone has > had success with FoxP3 yet on murine tissues, and what you could use > as a positive control. > > Thanks, > Melissa > ------------------------------ > > Message: 7 > Date: Mon, 31 Jul 2006 14:02:03 -0500 > From: "Drew Allan Roenneburg" > Subject: [Histonet] Foxp3 on Murine tissue > To: > Message-ID: <44CE0D5B0200009C00001F23@gw.surgery.wisc.edu> > Content-Type: text/plain; charset=US-ASCII > > Hi, I was wondering if anyone has stained for foxp3 on frozen or FFPE > mouse tissues (spleen). I have tried the Abcam rabbit anti-human > foxp3 antibody that works on FFPE human and monkey (tonsil and > spleen), however have had little success on mouse. Thanks Drew > Roenneburg > > > > ------------------------------ > > Message: 2 > Date: Tue, 1 Aug 2006 13:45:30 -0400 > From: "Pixley, Sarah \(pixleysk\)" > Subject: [Histonet] RE:BrdU and GFP staining > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Dear Steve: > I would stain for GFP with an antibody to GFP and do labeling with DAB. > I recommend the Elite ABC kits. The DAB is moderately resistant to the > BrdU penetration protocols. Then, fix your tissues well with > paraformaldehyde, i.e. 15 mins, 4%, and proceed with the BrdU, using a > system that labels with a fluorescent antibody. This will only work, > however, if your GFP is in different cells, or it is in the cytoplasm > of large cells that have no nuclear staining. The DAB can obscure the > secondary fluorescent labeling. > > Good luck. Stay Cool back at you. > Sarah Pixley, > Ohio > > Message: 2 > Date: Mon, 31 Jul 2006 10:22:41 -0700 (PDT) > From: Steven Coakley > Subject: [Histonet] BrdU and GFP expression > To: Histonet@lists.utsouthwestern.edu > Message-ID: <20060731172241.65699.qmail@web38215.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Good afternoon from WI, > > We have several FFPE blocks from 2003 that express GFP. Our lead > scientist would like to perform Brdu IHC in the same slides hoping to > express both. As expected the AR removes the GFP green label. Has > anyone any experience expressing Brdu and GFP on the same slide. > > Stay cool, > > Steve > > > > > > ------------------------------ > > Message: 3 > Date: Tue, 1 Aug 2006 13:49:31 -0400 > From: "Pixley, Sarah \(pixleysk\)" > Subject: [Histonet] Staining cultured cells > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > > To stain cultured cells we routinely fix with 4% paraformaldehyde for > 15-20 mins. But if the antigen is sensitive to aldehydes, then we fix > with ice-cold 100% methanol for 5-10 mins. Your acetone technique for > 30 mins is extremely harsh for cultured cells. If you want to continue > with it, I would fix with the acetone for only a few minutes and try that. > > As for the other person who asked about staining cultured cells, we do > exactly the same ICC as on sections, although we usually are able to > dilute the primary antibodies more than with frozen tissue sections > (using paraformaldehyde perfused tissues). > > Sarah Pixley > Ohio > > > Message: 4 > Date: Mon, 31 Jul 2006 13:41:54 -0400 > From: Christopher C Overend > Subject: [Histonet] IFA staining problems > To: histonet@lists.utsouthwestern.edu > Message-ID: <1fbaf901fb5b0e.1fb5b0e1fbaf90@huskymail.uconn.edu> > Content-Type: text/plain; charset=us-ascii > > I am new to the technique of immunofluorescence, and have been doing > some work with it lately on cultured cells. Specifically, I have been > staining intracellular virus with very good results. However, when i > try to detect cellular proteins, I do not get any staining. The > proceedure i have been using consists of "fixation" with 90% acetone > for 30 min, at 4degrees C. the rest of the process had been much like > an ELISA, all incubations have been for 30 min at 4degrees. The buffer > i have been using is PBS, 1%BSA, 0.1%NaN3. Someone mentioned the > problem could be from the acetone and suggested trying a glyoxal > fixative. Can anyone offer some insights, or if there is a different > protocol i might have to follow using a glyoxal fixative with tissue culture? > Thank you! > Chris > > Christopher Overend > Ph.D Student > University of Connecticut > Department of Pathobiology and Veterinary Sciences Storrs, CT > Christopher.overend@uconn.edu > > > > > > ------------------------------ > > Message: 4 > Date: Tue, 01 Aug 2006 13:23:06 -0500 > From: "Xilong Li" > Subject: [Histonet] immunohistochemical analysis for connective tissue > To: > Message-ID: <44CF55BA020000F000004E06@swnw124.swmed.edu> > Content-Type: text/plain; charset=US-ASCII > > Hi, All members, > > I am looking for a consistent protocol to stain collagen of connective > tissues in muscle frozen sections by immunohistochemical analysis > including information for primary antibody and second antibody used in > the protocols. > > Thanks in advance. > > xilong li > > Dr. Xilong Li > Hypertension Division, Internal Medicine University of Texas > Southwestern Medical Center > 5323 Harry Hiness Blvd-J4.142 > Dallas, TX 75390 > > Tel: 214-648-9966(L) > Fax: 214-648-7902 > > > > > ------------------------------ > > Message: 5 > Date: Tue, 1 Aug 2006 14:24:37 -0400 > From: Helen E Johnson > Subject: [Histonet] ImmunoVision Technologies, Co. > To: histonet@lists.utsouthwestern.edu > Message-ID: > > h.state.ny.us> > > Content-Type: text/plain; charset=US-ASCII > > > Hi Histonetters, > Does anyone know if ImmunoVision Technologies, Co. is still in > business? No one answers the phone ( 650-992-7563). > Helen > (hej01@health.state.ny.us) > > > > > ------------------------------ > > Message: 6 > Date: Tue, 1 Aug 2006 11:32:41 -0700 (PDT) > From: Ron Lagerquist > Subject: Re: [Histonet] ImmunoVision Technologies, Co. > To: Helen E Johnson , > histonet@lists.utsouthwestern.edu > Message-ID: <20060801183241.80617.qmail@web56106.mail.re3.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > They were purchased by VisionBiosystems (800-753-7264) > http://www.vision-bio.com/media_releases.html > > Ron > > Helen E Johnson wrote: > > Hi Histonetters, > Does anyone know if ImmunoVision Technologies, Co. is still in > business? No one answers the phone ( 650-992-7563). > Helen > (hej01@health.state.ny.us) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Do you Yahoo!? > Next-gen email? Have it all with the all-new Yahoo! Mail Beta. > > ------------------------------ > > Message: 7 > Date: Tue, 01 Aug 2006 14:33:51 -0400 > From: Pamela Marcum > Subject: [Histonet] Pennsylvania Histology Meeting for October 2006 > To: histonet@lists.utsouthwestern.edu > Message-ID: <6.1.1.1.2.20060801142646.01a26d88@mail.vet.upenn.edu> > Content-Type: text/plain; charset="us-ascii"; format=flowed > > > > Good Afternoon All, > > We have our web site up and partially up. We do have our program > online and have the program in the download file area for you to > review and if register. The program can be printed off. Just go to > www.pahisto.org and look for downloads in the top line. Click on and > open or save. The format is a Microsoft Word document. If you have a > problem or would need a pre-printing copy please let either me or > Gloria Limetti know. Gloria can be reached at glorialimetti@yahoo.com or 412-647-8535. > > Thanks for your patience and we will be updating the program with > vendors and other information as we near the meeting in October. > > > Best Regards, > > Pamela A Marcum > Manager, Histology Special Procedures > University of Pennsylvania > School of Veterinary Medicine > R.S. Reynolds Jr. CORL > New Bolton Center > 382 West Street Road > Kennett Square, PA 19348 > > Phone - 610-925-6278 > Fax - 610-925-8120 > E-mail - pmarcum@vet.upenn.edu > > > > > > ------------------------------ > > Message: 8 > Date: Wed, 2 Aug 2006 10:49:46 +1000 > From: "Tony Henwood" > Subject: RE: [Histonet] Ethanol/other fixations for frozen sections > To: "Andrea T. Hooper" , "Histonet" > > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Urgent Frozen Sections for H&E - we fix in methanol > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory > Manager & Senior Scientist The Children's Hospital at Westmead, Locked > Bag 4001, Westmead, 2145, AUSTRALIA. > Tel: 612 9845 3306 > Fax: 612 9845 3318 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea > T. Hooper > Sent: Monday, 31 July 2006 10:45 PM > To: Histonet > Subject: [Histonet] Ethanol/other fixations for frozen sections > > > Just taking a survey .... What are everyone's thoughts on using > ethanol for frozen sections? I have a colleague who swears by it but I > am an acetone person myself. > > Thoughts, comments, input on what is the best fix for frozen section > IHC? > > Thanks! > -- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************** > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the > individual sender, and are not necessarily the views of The Children's > Hospital at Westmead > > This note also confirms that this email message has been virus scanned > and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************** > > > > > ------------------------------ > > Message: 9 > Date: Tue, 1 Aug 2006 19:40:34 -0700 (PDT) > From: Cheryl > Subject: [Histonet] Looking for Florida histotechs--or people who are > interested in Florida relocation: we help with the license. > To: Histonet > Message-ID: <20060802024034.14424.qmail@web50904.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hello everyone-- > > Florida's registry process has created a major shortage of qualified techs in the state. We have a number of Florida permanent and temp opportunities. The permanent labs will consider transitional job situations to allow you to work while your license is processed. We also have a number of temp situations for a great pay scale for techs registered to work in the state. Travel isn't for everyone, but these are good labs and a great way to start. > > Either way, we'll help you through the process to get you through it as quickly as possible. If you're even curious, give us a call. Of course we have a number of other opportunities including sales and management all over the country. We won't work with every lab--those we place find themselves in jobs that fit--where they are happy to go to work. We're here to help and no fee to you. > > Tiffany is in the office and I'm traveling as a temp for a couple of weeks--as always, referral bonuses are available--share us with your friends! > > Tiffany/Office 281.852.9457 > Cheryl/Cell 281.883.7704 > or this number follows me around: 800.756.3309 (it will ring a long > time to find me--please be patient and leave your phone number!) > > We return all calls. > > Thanks! > > Cheryl > > > > Cheryl Kerry, HT(ASCP) > Full Staff Inc. > Staffing the AP Lab by helping one Tech at a time. > 281.883.7704 c > 281.852.9457 o > admin@fullstaff.org > > Sign up for the FREE monthly newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. > > ------------------------------ > > Message: 10 > Date: Wed, 2 Aug 2006 09:47:06 +0200 > From: "louise renton" > Subject: [Histonet] article from J of histotechnology > To: Histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Hi can anyone share a copy of this article? I would be very grateful > > "Entering the realm of mineralized bone processing: a review of the > literature and techniques." J Histotechnol 1997:20 (3) p259-266 > > Many thanks > > Louise Renton > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > "There are nights when the wolves are silent and only the moon howls". > George Carlin > No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > > > > ------------------------------ > > Message: 11 > Date: Wed, 2 Aug 2006 16:19:02 +0800 > From: Shirley PHUA > Subject: [Histonet] Zinc-Based Fixative > To: histonet@lists.utsouthwestern.edu > Message-ID: > > g> > > Content-Type: text/plain; charset="US-ASCII" > > Dear All, > > Reference Article: > Lab Invest 2003 Jun;83(6):889-99 > Zinc-based fixative improves preservation of genomic DNA and proteins > in histoprocessing of human tissues > > Anyone out there know of the exact composition of any zinc-based > fixative that is based on the following? > 1. 0.5% Zinc Chloride and > 2. 0.5% Zinc Acetate in 0.1M Tris base buffer containing 0.05% calcium > acetate > > > Many thanks & regards, > Shirley Phua > Histopathology Laboratory > Centre for Forensic Medicine > Health Sciences Authority > Singapore > > > > ------------------------------ > > Message: 12 > Date: Wed, 2 Aug 2006 08:23:30 -0500 > From: "Vickroy, Jim" > Subject: [Histonet] prefilled formalin containers > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > > > We have experienced problems with prefilled formalin containers that > we send to our clients. We have tried two different vendors and they > both leak. One of the container types claims to have an o-ring type > lid and the other does not have an o-ring. The containers with the > o-ring seems to leak as much as the others. In fact the o-ring type > sometimes leak when they are received from the vendor. If anyone has > dealt with this successfully please let me know how you solved it. > > > > Jim Vickroy > > Technical Supervisor - Surgical and Autopsy Pathology > > Memorial Medical Center > > > > > > This message (including any attachments) contains confidential > information intended for a specific individual and purpose, and is > protected by law. If you are not the intended recipient, you should > delete this message. Any disclosure, copying, or distribution of this message, or the taking of any action based on it, is strictly prohibited. > > > ------------------------------ > > Message: 13 > Date: Wed, 2 Aug 2006 08:41:32 -0500 > From: "Jackie M O'Connor" > Subject: Re: [Histonet] prefilled formalin containers > To: "Vickroy, Jim" > Cc: histonet@lists.utsouthwestern.edu, > histonet-bounces@lists.utsouthwestern.edu > Message-ID: > > > Content-Type: text/plain; charset="US-ASCII" > > Are they leaking on the return trip? Hard to get your clients to make > sure they are screwed on properly. Each vial/jar should be contained > within a secondary leakproof bag, anyway - to prevent leaks in transit. > Give Surgipath a call. > > Jackie O' > > > > "Vickroy, Jim" Sent by: > histonet-bounces@lists.utsouthwestern.edu > 08/02/2006 08:23 AM > > To > > cc > > Subject > [Histonet] prefilled formalin containers > > > > > > > > > We have experienced problems with prefilled formalin containers that > we send to our clients. We have tried two different vendors and they > both leak. One of the container types claims to have an o-ring type > lid and the other does not have an o-ring. The containers with the > o-ring seems to leak as much as the others. In fact the o-ring type > sometimes leak when they are received from the vendor. If anyone has > dealt with this successfully please let me know how you solved it. > > > > Jim Vickroy > > Technical Supervisor - Surgical and Autopsy Pathology > > Memorial Medical Center > > > > > > This message (including any attachments) contains confidential > information intended for a specific individual and purpose, and is > protected by law. If you are not the intended recipient, you should > delete this message. Any disclosure, copying, or distribution of this > message, or the taking of any action based on it, is strictly > prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 14 > Date: Wed, 2 Aug 2006 08:42:33 -0500 > From: "Ford Royer" > Subject: [Histonet] '07 TSH Meeting > To: > Message-ID: <003101c6b639$82553090$7701a80a@Ford> > Content-Type: text/plain; charset="us-ascii" > > Would the person(s) who are organizing next years Texas Society of > Histotechnology meeting please contact me? > > Thanks! > > ~ Ford > > Ford M. Royer, MT(ASCP) > Histology Product Manager > Minnesota Medical, Inc. > 7177 Madison Ave. W. > Golden Valley, MN 55427-3601 > CELL: 612-839-1046 > Phone: 763-542-8725 > Fax: 763-546-4830 > eMail: froyer@bitstream.net > > > > > ------------------------------ > > Message: 15 > Date: Wed, 2 Aug 2006 10:05:40 -0400 > From: "Santana, Diane" > Subject: [Histonet] The New VIP > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: <4C96AA62BADFD81180CB0002A5AD67FB07113AC5@MAILPMA> > Content-Type: text/plain; charset="iso-8859-1" > > I have a question and please nobody get all defensive about this, but has > anybody recently bought the VIP 5 tissue processor? If you have, do or did > you have any problems with it? > Thanks > Diane Santana > PMA > Haverhill, Mass. > > > > ------------------------------ > > Message: 16 > Date: Wed, 02 Aug 2006 10:31:26 -0400 > From: "Fred Underwood" > Subject: Re: [Histonet] The New VIP > To: , > Message-ID: > Content-Type: text/plain; charset=US-ASCII > > I've had mine for a year and (knock, knock on wood) have had no > problems. > > Fred > > >>> "Santana, Diane" 08/02 10:05 AM >>> > I have a question and please nobody get all defensive about this, but > has > anybody recently bought the VIP 5 tissue processor? If you have, do or > did > you have any problems with it? > Thanks > Diane Santana > PMA > Haverhill, Mass. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 17 > Date: Wed, 2 Aug 2006 09:36:48 -0500 > From: "Henry, Charlene" > Subject: RE: [Histonet] The New VIP. . > To: "Santana, Diane" , > > Message-ID: > <5CB39BCA5724F349BCB748675C6CA1A2099A1D6A@SJMEMXMB02.stjude.sjcrh.local> > > Content-Type: text/plain; charset="US-ASCII" > > We purchased a VIP5 a few years ago and had several problems so it was > replaced with a new VIP5. Since that time we have had no problems and it > has been a reliable instrument. > Charlene > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Santana, > Diane > Sent: Wednesday, August 02, 2006 9:06 AM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] The New VIP. . > > I have a question and please nobody get all defensive about this, but > has anybody recently bought the VIP 5 tissue processor? If you have, do > or did you have any problems with it? > Thanks > Diane Santana > PMA > Haverhill, Mass. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 18 > Date: Wed, 2 Aug 2006 10:30:16 -0500 > From: > Subject: Re: [Histonet] The New VIP > To: "Santana, Diane" , > "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > <20060802153018.PMMJ26069.dukecmmtao03.coxmail.com@dukecmmtao03> > Content-Type: text/plain; charset=ISO-8859-1 > > We have a VIP 5 that we purchased a little over a year ago. Have not had a single problem with it so far. > > Chris Rains > WPM Pathology Laboratory > Salina, KS > > > > From: "Santana, Diane" > > Date: 2006/08/02 Wed AM 09:05:40 CDT > > To: "'histonet@lists.utsouthwestern.edu'" > > Subject: [Histonet] The New VIP > > > > I have a question and please nobody get all defensive about this, but has > > anybody recently bought the VIP 5 tissue processor? If you have, do or did > > you have any problems with it? > > Thanks > > Diane Santana > > PMA > > Haverhill, Mass. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > Important: This email and any attachments may contain confidential information subject to protection under the Federal Standards for Privacy of Individually Identifiable Health Information (45 C.F.R. Parts 160 and 164). If you or your organization is a "Covered Entity" under the above mentioned regulations, you are obligated to treat such information in a manner consistent with the regulations. If it appears that this email was sent to you in error, (1) you are prohibited from utilizing or disseminating this email or any attachments; (2) please immediately delete it from your computer and any servers or other locations where it might be stored and email (crains@wpmpath.com) or call (Chris Rains) at 785-823-7201 advising that you have done so. We appreciate your cooperation. > > > > > ------------------------------ > > Message: 19 > Date: Wed, 2 Aug 2006 11:42:17 -0400 > From: "Stahl, Michael" > Subject: [Histonet] prefilled formalin containers > To: > Message-ID: > <4C878E714B21EB4F8EB159777B8822EE5B6834@bvfyms01.net.bvha.org> > Content-Type: text/plain; charset="us-ascii" > > We use a Surgipath (cat #008100 which are the pre-filled 60ml jars. We > found these to be almost leak proof. However, they do leak when the jars > arnt screwed on correctly. But we agree, its not pleasant to have a > specimen jar in a bio hazard bag full of fixative. Just try to find that > cervial biospy! > > Michael P. Stahl HT (ASCP) > BVRHC > 145 W Wallace St > Findlay, OH 45840 > mstahl@bhva.org > > > ------------------------------ > > Message: 20 > Date: Wed, 02 Aug 2006 08:55:45 -0700 > From: Andrea Grantham > Subject: Re: [Histonet] The New VIP > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > <4.3.2.7.2.20060802085142.00cec460@algranth.inbox.email.arizona.edu> > Content-Type: text/plain; charset="us-ascii"; format=flowed > > I just got a new VIP 5 and I love it. Wanted one all my (histotech) life > and I'm so happy to finally have it! It has been great. So easy to use and > the tissues are coming out wonderful. Knock-on-wood it will do as well as > the old Tissue Tek dipper/dunker that it is replacing. > Andi > > > At 10:30 AM 8/2/2006 -0500, crains@wpmpath.com wrote: > >We have a VIP 5 that we purchased a little over a year ago. Have not had > >a single problem with it so far. > > > >Chris Rains > >WPM Pathology Laboratory > >Salina, KS > > > > > > From: "Santana, Diane" > > > Date: 2006/08/02 Wed AM 09:05:40 CDT > > > To: "'histonet@lists.utsouthwestern.edu'" > > > > > Subject: [Histonet] The New VIP > > > > > > I have a question and please nobody get all defensive about this, but has > > > anybody recently bought the VIP 5 tissue processor? If you have, do or did > > > you have any problems with it? > > > Thanks > > > Diane Santana > > > PMA > > > Haverhill, Mass. > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > >Important: This email and any attachments may contain confidential > >information subject to protection under the Federal Standards for Privacy > >of Individually Identifiable Health Information (45 C.F.R. Parts 160 and > >164). If you or your organization is a "Covered Entity" under the above > >mentioned regulations, you are obligated to treat such information in a > >manner consistent with the regulations. If it appears that this email was > >sent to you in error, (1) you are prohibited from utilizing or > >disseminating this email or any attachments; (2) please immediately delete > >it from your computer and any servers or other locations where it might be > >stored and email (crains@wpmpath.com) or call (Chris Rains) at > >785-823-7201 advising that you have done so. We appreciate your cooperation. > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ..................................................................... > : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : > : Sr. Research Specialist University of Arizona : > : (office: AHSC 4212) P.O. Box 245044 : > : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : > : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : > :...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > > > > ------------------------------ > > Message: 21 > Date: Wed, 2 Aug 2006 11:08:00 -0500 > From: "Vickroy, Jim" > Subject: [Histonet] charging and CPT questions > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > > > Can anyone shed any light on the proper way to code and charge the > following: > > > > 1. Muscle Biopsy Histochemistry - Frozen sections -88314 or 88319? > I'm not sure which one. > > > > 2. Touch preps - Done when we do a frozen section. The frozen > section is 88331 and the touch prep should be 88333 or 88334? > > > > 3. Semi-thin sectioning of nerve biopsies - Plastic embedding > In the past we charged all nerve bxs with an 88348 (EM) charge since > > not only did we do the semi-thin sectioning but we > also did the transmission EM. Our neuropathologist now wants the > semi-thin sections > > routinely but not the rest of the EM examination. > > > > Thanks > > > > Jim Vickroy > > Technical Supervisor - Surgical and Autopsy Pathology > > Memorial Medical Center > > > > > > This message (including any attachments) contains confidential information intended for a > specific individual and purpose, and is protected by law. If you are not the intended recipient, > you should delete this message. Any disclosure, copying, or distribution of this message, or the > taking of any action based on it, is strictly prohibited. > > > ------------------------------ > > Message: 22 > Date: Wed, 2 Aug 2006 09:45:21 -0700 > From: "Jayne Scoggin" > Subject: [Histonet] please unsubscribe me from the list > To: > Message-ID: <200608021624.k72GO3Vh004624@mx.bioceptlabs.com> > Content-Type: text/plain; charset="us-ascii" > > Please unsubscribe me from the list. > > Jayne Scoggin, Biocept Inc. > 858 320 8224 > jscoggin@biocept.com > > > > > > ------------------------------ > > Message: 23 > Date: Wed, 2 Aug 2006 12:40:55 -0400 > From: "Zajic Kari" > Subject: RE: [Histonet] charging and CPT questions > To: "Vickroy, Jim" , > > Message-ID: > <095327C7CDBDF64B9E9728A54799091E015CC5A2@ORLEV03.hca.corpad.net> > Content-Type: text/plain; charset="iso-8859-1" > > Hi Jim. I do not do muscle or nerve at my facility but we send to the University of Miami and they charge the following: > > Muscle > 88305 x1: Surgical Path,gross,micro > 88313 x2: Paraffin: H&E Plastic > 88314 x2: cryostat H&E Trichrome > 88319 x8: Enzyme HistoChem: NADH,Esterase, ATPasex3,Cox, Cox & SDH, SDH > > Nerve > 88305 x1: Surg Path,gross,micro > 88313 x6: Paraffin H&E,Trichrome,Luxol Fast Blue,Toludine Blue, Crystal Violet,Silver Stain > 88314 x2: Cryostat: H&E,Trichrome > 88319 x1: Frozen Section Histochem: Esterase > 88348 x1: Nerve Electron Micro > 88356 x1: Nerve Morphometry > 88362 x1: Nerve Teased Preps > > As far as frozen section touch preps, they came out with new CPT's in 2006 as follows: > 88333 Path consult during surgery (cyto exam) initial site ie.touch prep,squash prep > physician fee global $75-100+ > technical $17-25 > 88334 cyto exam, EACH ADDITIONAL SITE, touch prep,squash prep > physicain fee $40-50+ > technical $10-15 > this info was given to me by Health Systems Concepts,Inc. > > hope this helps!!! > Kari :) > > Kari Marie Zajic HT,MLT > Histology Supervisor > Palms West Hospital > Pathology Department > 13001 State Road Eighty > Loxahatchee, Florida 33470 > phone: (561)798-6036 > telefax: (561)753-4298 > voicemail: (561)753-4299 > pager: (561)610-4949 > email: Kari.Zajic@HCAHealthcare.com > > This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL > information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Vickroy, > Jim > Sent: Wednesday, August 02, 2006 12:08 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] charging and CPT questions > > > > > Can anyone shed any light on the proper way to code and charge the > following: > > > > 1. Muscle Biopsy Histochemistry - Frozen sections -88314 or 88319? > I'm not sure which one. > > > > 2. Touch preps - Done when we do a frozen section. The frozen > section is 88331 and the touch prep should be 88333 or 88334? > > > > 3. Semi-thin sectioning of nerve biopsies - Plastic embedding > In the past we charged all nerve bxs with an 88348 (EM) charge since > > not only did we do the semi-thin sectioning but we > also did the transmission EM. Our neuropathologist now wants the > semi-thin sections > > routinely but not the rest of the EM examination. > > > > Thanks > > > > Jim Vickroy > > Technical Supervisor - Surgical and Autopsy Pathology > > Memorial Medical Center > > > > > > This message (including any attachments) contains confidential information intended for a > specific individual and purpose, and is protected by law. If you are not the intended recipient, > you should delete this message. Any disclosure, copying, or distribution of this message, or the > taking of any action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 33, Issue 2 > *************************************** > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From anh2006 <@t> med.cornell.edu Wed Aug 2 22:07:26 2006 From: anh2006 <@t> med.cornell.edu (Andrea Hooper) Date: Wed Aug 2 22:07:31 2006 Subject: [Histonet] Endothelial Cell Markers and Cell Staining Message-ID: DAKO's anti-CD31 clone JC/70 is EXCELLENT for paraffin section staining. I am glad to share my protocol with anyone who is interested, just mail me offline. Both DAKO's JC70 and Pharmingen's WM59 are also excellent for frozen sections. ----- Original Message ----- From: Donna Harclerode Date: Wednesday, August 2, 2006 9:52 pm Subject: [Histonet] Endothelial Cell Markers and Cell Staining > Hi Hannah > I do lots of human endothelial staining and my best all around general > endothelial marker is CD 31 from PharMingen, clone WM59 cat # > 550389. I > use it in frozen sections (1:40 with fluorescent secondary or Labeled > step avidin biotin and DAB)fixed for 5 minutes in 75% acetone 25% > ethanol (Thanks for this fix recommendation years ago Gayle > Callas!) or > in cells fixed 15 minutes in 4%PFA. I usually incubate primaries > overnight at 4oC, but also sometimes do 1-2 hours on the counter with > rocking. My other endothelial markers are also from PharMingen and > somework in paraffin (sort of) but work great in frozen and cells. > HumanCD34, CD105, CD106 and CD144 are all very nice markers for > differenttypes of endothelial cells with the same fixation in both > cells and > frozen sections. PharMingen makes excellent endothelial markers for > mouse and rat too. The Rat CD 31 from PharMingen cross reacts in > pig if > anyone cares. I have been staining lots of tissue culture plates and > chamber slides with these markers double labeled with smooth muscle > myosin or vWF and DAPI nuclei. I do not like the 12 well plates and > muchprefer the 24. For some reason the 12 well plates that we have > arehydrophobic and I have problems covering the cells. In a 24 > well plate > I only use about 100ul per well and it works nicely > I would not recommend most of PharMingen endothelial markers for > paraffin sections. > > Christopher > > I noticed a couple things that might help your staining. I would not > use sodium azide in an antibody diluent for staining- the amount > may not > hurt, but it sure does not help. I use azide in stock antibody if they > do not come in with azide, but would not add more when making up > for a > IHC (or ICC, if you prefer staining solution) I also do not use BSA > anymore- I have found my best results are when I use 2-5% of whatever > normal serum from species your secondary is made in and not use > BSA. You > also have no surfactant of any type to help get through the cell > membrane. This last one would be my first guess as the problem. There > are also antibodies that do not work well in cells and they work great > in paraffin sections- I have found a couple that I have rejected for > cells and frozen that are great in paraffin sections. So much > depends on > your antibody- they do not all cooperate in all formats I want them to > work in. > > You can make your own diluent with Tween or Triton X 100 but I highly > recommend the prepared diluents. I think there may be some casein in > them, but do not know the secret formula. I use Dako diluent cat > S0809for all my primary and secondary dilutions. (I add 2% normal > donkeyserum in the primary only because all my secondaries are made > in donkey) > My isotype specific secondaries are the only exception - they are all > made in goat so I would use normal goat if I am doing multi color all > mouse, but different isotype abs. > I used Dako diluent when optimizing the PharMingen antibodies years > ago(I set up the original IHC testing and QC specs for all PharMingen > antibodies) - I would expect other companies' diluent to work well. > One > thing I have noticed is in antibodies that specifically say to "use no > Triton in your antibody diluent" - the Dako diluent still works great. > I have complete penetration of cells and do not damage any of the > sensitive markers for special antibodies. I have never found a > primaryantibody that works better in another homemade diluent other > than Dako. > > I have a protocol for plates or chamber slides I can email it if you > want it. > > Good Luck > > > Donna Harclerode, HT, (ASCP), HTL, QIHC > Scientist / Immunohistochemistry > Cytori Therapeutics > 3020 Callan Rd. > San Diego, CA 92121 > 858-458-0900 ext 5416 > fax 858 200-0945 > dharclerode@cytoritx.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From Karen.Heckford <@t> CHW.edu Thu Aug 3 08:08:28 2006 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Thu Aug 3 08:08:44 2006 Subject: [Histonet] Workload Message-ID: Hello Everyone, I was wondering if someone could give me some feedback on some questions. Do you think 80-100 cassettes a day is too much for one tech in a 8 hour period? We are looking at only a few special stains and probably not a lot of recuts. No IHC's. I am trying to figure out schedules and perhaps hiring a part timer. H&E will be continuous feed autostainer and special stains will be done by hand. If you did have 2 techs. could they reasonably get 80-100 cassettes done from embedding to coverslipped(done by hand) in 4 hours. I am right now a one person show thinking about expanding. Cheers, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathololgy Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu From Mary.Vaughan <@t> RoswellPark.org Thu Aug 3 08:49:31 2006 From: Mary.Vaughan <@t> RoswellPark.org (Vaughan, Mary) Date: Thu Aug 3 08:49:05 2006 Subject: [Histonet] re:TUNEL kits Message-ID: <6FF91AE4F1DC7743A6466E334EB865AE0BE0F3D3@VERITY.roswellpark.org> The Chemicon/Serologicals [cat #S7101] TUNEL kit works very well. I usually don't use kits, but it's such a fussy assay.... this one is reproducible and consistent. Best Regards, Mary Vaughan HT (ASCP) Research Specialist Roswell Park Cancer Institute ASB-212 Elm & Carlton Streets Buffalo, N Y 14263 phone: 716.845.3006 FAX: 716.845.3489 email: mary.vaughan@roswellpark.org This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. From rjbuesa <@t> yahoo.com Thu Aug 3 09:24:09 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Aug 3 09:24:14 2006 Subject: [Histonet] Workload In-Reply-To: Message-ID: <20060803142410.58692.qmail@web61219.mail.yahoo.com> Karen: 80-100 cassettes/day is way below average. Under separate cover I am sending you an article I just published in Advance for MLP (3 July/06) that will give you some idea of averages for all histology tasks (both licensed and unlicensed). Ren? J. "Heckford, Karen - SMMC-SF" wrote: Hello Everyone, I was wondering if someone could give me some feedback on some questions. Do you think 80-100 cassettes a day is too much for one tech in a 8 hour period? We are looking at only a few special stains and probably not a lot of recuts. No IHC's. I am trying to figure out schedules and perhaps hiring a part timer. H&E will be continuous feed autostainer and special stains will be done by hand. If you did have 2 techs. could they reasonably get 80-100 cassettes done from embedding to coverslipped(done by hand) in 4 hours. I am right now a one person show thinking about expanding. Cheers, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathololgy Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Next-gen email? Have it all with the all-new Yahoo! Mail Beta. From Kari.Zajic <@t> HCAhealthcare.com Thu Aug 3 09:50:51 2006 From: Kari.Zajic <@t> HCAhealthcare.com (Zajic Kari) Date: Thu Aug 3 09:50:59 2006 Subject: [Histonet] Workload In-Reply-To: <20060803142410.58692.qmail@web61219.mail.yahoo.com> Message-ID: <095327C7CDBDF64B9E9728A54799091E015CC5A8@ORLEV03.hca.corpad.net> Hi Karen. I had a similar situation in my lab. In my opinion, 2 techs is sufficient assuming you do not do alot of frozen sections, secretarial work, etc. We had 2 full time techs, did approximately 100-150 blocks a day (full processing/embedding), all special stains by hand, manual coverslipping, automated stainer and were done in 4.5 hours (typically). Now, the problem we ran into was all of the "extra" technical work needed in the lab, accessioning, grossing, frozen sections, send-outs, filing, reagent change, processing cytology cases (5-10 daily), etc. This was alot of extra work (our frozens were about 3-5 cases daily with 2 or more blocks on ea case) to put on 2 techs, not to mention all of the "supervisory" details done by one of the techs doing the benchwork. One of us had to come in at 6am to get the morning work out which left the other "late" tech alone after 2:30pm. Our operating room hours will run anywhere from 6a-9p with emergencies added in between...this left alot of late frozen sections in which we alternated the on-call. So, in my opinion, and I worked ALOT of OT in those days, if you have all those other duties, 2 techs is not sufficient. I needed a third part-timer to pick up the slack. If you are just doing what you stated (cutting/staining) you are ok. Again, just my opinion... Kari Marie Zajic HT,MLT Histology Supervisor Palms West Hospital Pathology Department 13001 State Road Eighty Loxahatchee, Florida 33470 phone: (561)798-6036 telefax: (561)753-4298 voicemail: (561)753-4299 pager: (561)610-4949 email: Kari.Zajic@HCAHealthcare.com This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Rene J Buesa Sent: Thursday, August 03, 2006 10:24 AM To: Heckford, Karen - SMMC-SF; Histonet (E-mail) Subject: Re: [Histonet] Workload Karen: 80-100 cassettes/day is way below average. Under separate cover I am sending you an article I just published in Advance for MLP (3 July/06) that will give you some idea of averages for all histology tasks (both licensed and unlicensed). Ren? J. "Heckford, Karen - SMMC-SF" wrote: Hello Everyone, I was wondering if someone could give me some feedback on some questions. Do you think 80-100 cassettes a day is too much for one tech in a 8 hour period? We are looking at only a few special stains and probably not a lot of recuts. No IHC's. I am trying to figure out schedules and perhaps hiring a part timer. H&E will be continuous feed autostainer and special stains will be done by hand. If you did have 2 techs. could they reasonably get 80-100 cassettes done from embedding to coverslipped(done by hand) in 4 hours. I am right now a one person show thinking about expanding. Cheers, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathololgy Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Next-gen email? Have it all with the all-new Yahoo! Mail Beta. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Amanda.Garcia <@t> TriadHospitals.com Thu Aug 3 10:00:52 2006 From: Amanda.Garcia <@t> TriadHospitals.com (Garcia, Amanda) Date: Thu Aug 3 10:02:47 2006 Subject: [Histonet] Taining checklists Message-ID: <8B63039C9DF4554C8FDBF31235F0E14801C54D42@CPRTEVS02.triadhospitals.net> Good morning to all, Will anyone out there be willing to share a checklist used in training grossing techs? Your help would be much appreciated. Thanks in advance Amy > Amanda (Amy) Garcia > Histology/Pathology > College Station Medical Center > (979) 680-5372 office > (979) 696-5446 fax > *Mailto:amanda.garcia@triadhospitals.com > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL information and may be read or used only by the intended > recipient. If you are not the intended recipient of the e-mail or any > of its attachments, please be advised that you have received this > e-mail in error and that any use, dissemination, distribution, > forwarding, printing, or copying of this e-mail or any attached files > is strictly prohibited. If you have received this e-mail in error, > please immediately purge it and all attachments and notify the sender > by reply e-mail or contact the sender at the number listed. > > > From ander093 <@t> tc.umn.edu Thu Aug 3 10:06:44 2006 From: ander093 <@t> tc.umn.edu (LuAnn Anderson) Date: Thu Aug 3 10:06:52 2006 Subject: [Histonet] Mohs Tech salaries In-Reply-To: <001f01c6b6a2$f461dfd0$6ca3ff44@HPPav2> References: <20060802232644.FGSK28894.ispmxmta05-srv.windstream.net@webmail-relay.alltel.net> <001f01c6b6a2$f461dfd0$6ca3ff44@HPPav2> Message-ID: <6.2.3.4.0.20060803100206.01d535c8@ander093.email.umn.edu> Wow--this survey is way lower than the one Advance conducted last year!! It was published in the 12/7/2005 issue and can be found online. It seems much more realistic, given the current circumstances in the field. The ASCP survey always seems to be on the low side for salaries. I have always questioned who they send the surveys out to--but have not gotten an answer on that as of yet!! At 09:17 PM 8/2/2006, you wrote: >Not specifically for Mohs, but I thought I would let Histonetters know that >the 2005 ASCP Wage and Vacancy survey results are now on the ASCP BOR >webpage: > >http://www.ascp.org/Certification/ForProgramDirectors/research/default.aspx > >Click on 2005. > >Survey done Nov. 2005, will be published in the August 2006 "Laboratory >Medicine". Found it on their website last week. > >Peggy A. Wenk, HTL(ASCP)SLS >William Beaumont Hospital >Royal Oak, MI 48073 > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >nsdd114@alltel.net >Sent: Wednesday, August 02, 2006 7:27 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Mohs Tech salaries > >I was wondering if those of you who are doing Mohs would respond to my >question. I am HT-ASCP certified and have 15 years in the field. I am >currently doing Mohs, because the hours and the pay are better in Mohs in my >area. I like doing Mohs and we keep very busy. We started the lab a year >ago and have one Mohs surgeon and are currently doing about 1500 cases a >year. Of course that is cases not blocks, because we frequently do cases >with anywhere from 6-25 pieces. Our practice would like to expand our >service and we have tried three techs, all on the job trained and not >certified. I personally know techs who were OJT trained who are top notch >techs. Two of the techs we interviewed weren't sufficiently skilled to work >really independently at high volume and trouble shoot routines. One didn't >know how to tell if she had a complete skin edge under the scope and she had >3 years of experience. That's scarey. The 3rd tech is pretty capable, but >is also a CMA and prefers the nursing position. My question is "Does anyone >know of a Mohs salary survey or any guidelines as far as pay goes for both >certified and non-certified techs?" I personally don't care if someone is >confident and capable whether they are certified or not since it isn't >required at this time, but we do want to establish a pay scale that rewards >people based on their overall training and skill. I do suspect that the >next few years will bring some kind of standardized training requirements >and/or certification for Mohs techs. If anyone has any input I'd appreciate >it. We are in the south central east coast area. Thanks, Nancy > > > > From: histonet-request@lists.utsouthwestern.edu > > Date: 2006/08/02 Wed PM 12:01:20 CDT > > To: histonet@lists.utsouthwestern.edu > > Subject: Histonet Digest, Vol 33, Issue 2 > > > > Send Histonet mailing list submissions to > > histonet@lists.utsouthwestern.edu > > > > To subscribe or unsubscribe via the World Wide Web, visit > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > or, via email, send a message with subject or body 'help' to > > histonet-request@lists.utsouthwestern.edu > > > > You can reach the person managing the list at > > histonet-owner@lists.utsouthwestern.edu > > > > When replying, please edit your Subject line so it is more specific > > than "Re: Contents of Histonet digest..." > > > > > > Today's Topics: > > > > 1. RE: Foxp3 on Murine tissue (Melissa Gonzalez) > > 2. RE:BrdU and GFP staining (Pixley, Sarah (pixleysk)) > > 3. Staining cultured cells (Pixley, Sarah (pixleysk)) > > 4. immunohistochemical analysis for connective tissue (Xilong Li) > > 5. ImmunoVision Technologies, Co. (Helen E Johnson) > > 6. Re: ImmunoVision Technologies, Co. (Ron Lagerquist) > > 7. Pennsylvania Histology Meeting for October 2006 (Pamela Marcum) > > 8. RE: Ethanol/other fixations for frozen sections (Tony Henwood) > > 9. Looking for Florida histotechs--or people who are interested > > in Florida relocation: we help with the license. (Cheryl) > > 10. article from J of histotechnology (louise renton) > > 11. Zinc-Based Fixative (Shirley PHUA) > > 12. prefilled formalin containers (Vickroy, Jim) > > 13. Re: prefilled formalin containers (Jackie M O'Connor) > > 14. '07 TSH Meeting (Ford Royer) > > 15. The New VIP (Santana, Diane) > > 16. Re: The New VIP (Fred Underwood) > > 17. RE: The New VIP. . (Henry, Charlene) > > 18. Re: The New VIP (crains@wpmpath.com) > > 19. prefilled formalin containers (Stahl, Michael) > > 20. Re: The New VIP (Andrea Grantham) > > 21. charging and CPT questions (Vickroy, Jim) > > 22. please unsubscribe me from the list (Jayne Scoggin) > > 23. RE: charging and CPT questions (Zajic Kari) > > > > > > ---------------------------------------------------------------------- > > > > Message: 1 > > Date: Tue, 1 Aug 2006 10:18:52 -0700 > > From: "Melissa Gonzalez" > > Subject: [Histonet] RE: Foxp3 on Murine tissue > > To: > > Message-ID: > > > > Content-Type: text/plain; charset="us-ascii" > > > > I've also tried several of the products out there with no success on > > murine tissues as well. I would be interested in hearing if anyone has > > had success with FoxP3 yet on murine tissues, and what you could use > > as a positive control. > > > > Thanks, > > Melissa > > ------------------------------ > > > > Message: 7 > > Date: Mon, 31 Jul 2006 14:02:03 -0500 > > From: "Drew Allan Roenneburg" > > Subject: [Histonet] Foxp3 on Murine tissue > > To: > > Message-ID: <44CE0D5B0200009C00001F23@gw.surgery.wisc.edu> > > Content-Type: text/plain; charset=US-ASCII > > > > Hi, I was wondering if anyone has stained for foxp3 on frozen or FFPE > > mouse tissues (spleen). I have tried the Abcam rabbit anti-human > > foxp3 antibody that works on FFPE human and monkey (tonsil and > > spleen), however have had little success on mouse. Thanks Drew > > Roenneburg > > > > > > > > ------------------------------ > > > > Message: 2 > > Date: Tue, 1 Aug 2006 13:45:30 -0400 > > From: "Pixley, Sarah \(pixleysk\)" > > Subject: [Histonet] RE:BrdU and GFP staining > > To: > > Message-ID: > > > > Content-Type: text/plain; charset="us-ascii" > > > > Dear Steve: > > I would stain for GFP with an antibody to GFP and do labeling with DAB. > > I recommend the Elite ABC kits. The DAB is moderately resistant to the > > BrdU penetration protocols. Then, fix your tissues well with > > paraformaldehyde, i.e. 15 mins, 4%, and proceed with the BrdU, using a > > system that labels with a fluorescent antibody. This will only work, > > however, if your GFP is in different cells, or it is in the cytoplasm > > of large cells that have no nuclear staining. The DAB can obscure the > > secondary fluorescent labeling. > > > > Good luck. Stay Cool back at you. > > Sarah Pixley, > > Ohio > > > > Message: 2 > > Date: Mon, 31 Jul 2006 10:22:41 -0700 (PDT) > > From: Steven Coakley > > Subject: [Histonet] BrdU and GFP expression > > To: Histonet@lists.utsouthwestern.edu > > Message-ID: <20060731172241.65699.qmail@web38215.mail.mud.yahoo.com> > > Content-Type: text/plain; charset=iso-8859-1 > > > > Good afternoon from WI, > > > > We have several FFPE blocks from 2003 that express GFP. Our lead > > scientist would like to perform Brdu IHC in the same slides hoping to > > express both. As expected the AR removes the GFP green label. Has > > anyone any experience expressing Brdu and GFP on the same slide. > > > > Stay cool, > > > > Steve > > > > > > > > > > > > ------------------------------ > > > > Message: 3 > > Date: Tue, 1 Aug 2006 13:49:31 -0400 > > From: "Pixley, Sarah \(pixleysk\)" > > Subject: [Histonet] Staining cultured cells > > To: > > Message-ID: > > > > Content-Type: text/plain; charset="us-ascii" > > > > > > To stain cultured cells we routinely fix with 4% paraformaldehyde for > > 15-20 mins. But if the antigen is sensitive to aldehydes, then we fix > > with ice-cold 100% methanol for 5-10 mins. Your acetone technique for > > 30 mins is extremely harsh for cultured cells. If you want to continue > > with it, I would fix with the acetone for only a few minutes and try that. > > > > As for the other person who asked about staining cultured cells, we do > > exactly the same ICC as on sections, although we usually are able to > > dilute the primary antibodies more than with frozen tissue sections > > (using paraformaldehyde perfused tissues). > > > > Sarah Pixley > > Ohio > > > > > > Message: 4 > > Date: Mon, 31 Jul 2006 13:41:54 -0400 > > From: Christopher C Overend > > Subject: [Histonet] IFA staining problems > > To: histonet@lists.utsouthwestern.edu > > Message-ID: <1fbaf901fb5b0e.1fb5b0e1fbaf90@huskymail.uconn.edu> > > Content-Type: text/plain; charset=us-ascii > > > > I am new to the technique of immunofluorescence, and have been doing > > some work with it lately on cultured cells. Specifically, I have been > > staining intracellular virus with very good results. However, when i > > try to detect cellular proteins, I do not get any staining. The > > proceedure i have been using consists of "fixation" with 90% acetone > > for 30 min, at 4degrees C. the rest of the process had been much like > > an ELISA, all incubations have been for 30 min at 4degrees. The buffer > > i have been using is PBS, 1%BSA, 0.1%NaN3. Someone mentioned the > > problem could be from the acetone and suggested trying a glyoxal > > fixative. Can anyone offer some insights, or if there is a different > > protocol i might have to follow using a glyoxal fixative with tissue >culture? > > Thank you! > > Chris > > > > Christopher Overend > > Ph.D Student > > University of Connecticut > > Department of Pathobiology and Veterinary Sciences Storrs, CT > > Christopher.overend@uconn.edu > > > > > > > > > > > > ------------------------------ > > > > Message: 4 > > Date: Tue, 01 Aug 2006 13:23:06 -0500 > > From: "Xilong Li" > > Subject: [Histonet] immunohistochemical analysis for connective tissue > > To: > > Message-ID: <44CF55BA020000F000004E06@swnw124.swmed.edu> > > Content-Type: text/plain; charset=US-ASCII > > > > Hi, All members, > > > > I am looking for a consistent protocol to stain collagen of connective > > tissues in muscle frozen sections by immunohistochemical analysis > > including information for primary antibody and second antibody used in > > the protocols. > > > > Thanks in advance. > > > > xilong li > > > > Dr. Xilong Li > > Hypertension Division, Internal Medicine University of Texas > > Southwestern Medical Center > > 5323 Harry Hiness Blvd-J4.142 > > Dallas, TX 75390 > > > > Tel: 214-648-9966(L) > > Fax: 214-648-7902 > > > > > > > > > > ------------------------------ > > > > Message: 5 > > Date: Tue, 1 Aug 2006 14:24:37 -0400 > > From: Helen E Johnson > > Subject: [Histonet] ImmunoVision Technologies, Co. > > To: histonet@lists.utsouthwestern.edu > > Message-ID: > > > > > h.state.ny.us> > > > > Content-Type: text/plain; charset=US-ASCII > > > > > > Hi Histonetters, > > Does anyone know if ImmunoVision Technologies, Co. is still in > > business? No one answers the phone ( 650-992-7563). > > Helen > > (hej01@health.state.ny.us) > > > > > > > > > > ------------------------------ > > > > Message: 6 > > Date: Tue, 1 Aug 2006 11:32:41 -0700 (PDT) > > From: Ron Lagerquist > > Subject: Re: [Histonet] ImmunoVision Technologies, Co. > > To: Helen E Johnson , > > histonet@lists.utsouthwestern.edu > > Message-ID: <20060801183241.80617.qmail@web56106.mail.re3.yahoo.com> > > Content-Type: text/plain; charset=iso-8859-1 > > > > They were purchased by VisionBiosystems (800-753-7264) > > http://www.vision-bio.com/media_releases.html > > > > Ron > > > > Helen E Johnson wrote: > > > > Hi Histonetters, > > Does anyone know if ImmunoVision Technologies, Co. is still in > > business? No one answers the phone ( 650-992-7563). > > Helen > > (hej01@health.state.ny.us) > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > --------------------------------- > > Do you Yahoo!? > > Next-gen email? Have it all with the all-new Yahoo! Mail Beta. > > > > ------------------------------ > > > > Message: 7 > > Date: Tue, 01 Aug 2006 14:33:51 -0400 > > From: Pamela Marcum > > Subject: [Histonet] Pennsylvania Histology Meeting for October 2006 > > To: histonet@lists.utsouthwestern.edu > > Message-ID: <6.1.1.1.2.20060801142646.01a26d88@mail.vet.upenn.edu> > > Content-Type: text/plain; charset="us-ascii"; format=flowed > > > > > > > > Good Afternoon All, > > > > We have our web site up and partially up. We do have our program > > online and have the program in the download file area for you to > > review and if register. The program can be printed off. Just go to > > www.pahisto.org and look for downloads in the top line. Click on and > > open or save. The format is a Microsoft Word document. If you have a > > problem or would need a pre-printing copy please let either me or > > Gloria Limetti know. Gloria can be reached at glorialimetti@yahoo.com or >412-647-8535. > > > > Thanks for your patience and we will be updating the program with > > vendors and other information as we near the meeting in October. > > > > > > Best Regards, > > > > Pamela A Marcum > > Manager, Histology Special Procedures > > University of Pennsylvania > > School of Veterinary Medicine > > R.S. Reynolds Jr. CORL > > New Bolton Center > > 382 West Street Road > > Kennett Square, PA 19348 > > > > Phone - 610-925-6278 > > Fax - 610-925-8120 > > E-mail - pmarcum@vet.upenn.edu > > > > > > > > > > > > ------------------------------ > > > > Message: 8 > > Date: Wed, 2 Aug 2006 10:49:46 +1000 > > From: "Tony Henwood" > > Subject: RE: [Histonet] Ethanol/other fixations for frozen sections > > To: "Andrea T. Hooper" , "Histonet" > > > > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > > > Urgent Frozen Sections for H&E - we fix in methanol > > > > Regards > > > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory > > Manager & Senior Scientist The Children's Hospital at Westmead, Locked > > Bag 4001, Westmead, 2145, AUSTRALIA. > > Tel: 612 9845 3306 > > Fax: 612 9845 3318 > > > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea > > T. Hooper > > Sent: Monday, 31 July 2006 10:45 PM > > To: Histonet > > Subject: [Histonet] Ethanol/other fixations for frozen sections > > > > > > Just taking a survey .... What are everyone's thoughts on using > > ethanol for frozen sections? I have a colleague who swears by it but I > > am an acetone person myself. > > > > Thoughts, comments, input on what is the best fix for frozen section > > IHC? > > > > Thanks! > > -- > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ********************************************************************** > > This email and any files transmitted with it are confidential and intended >solely for the use of the individual or entity to whom they are addressed. >If you are not the intended recipient, please delete it and notify the >sender. > > > > Views expressed in this message and any attachments are those of the > > individual sender, and are not necessarily the views of The Children's > > Hospital at Westmead > > > > This note also confirms that this email message has been virus scanned > > and although no computer viruses were detected, the Childrens Hospital at >Westmead accepts no liability for any consequential damage resulting from >email containing computer viruses. > > ********************************************************************** > > > > > > > > > > ------------------------------ > > > > Message: 9 > > Date: Tue, 1 Aug 2006 19:40:34 -0700 (PDT) > > From: Cheryl > > Subject: [Histonet] Looking for Florida histotechs--or people who are > > interested in Florida relocation: we help with the license. > > To: Histonet > > Message-ID: <20060802024034.14424.qmail@web50904.mail.yahoo.com> > > Content-Type: text/plain; charset=iso-8859-1 > > > > Hello everyone-- > > > > Florida's registry process has created a major shortage of qualified >techs in the state. We have a number of Florida permanent and temp >opportunities. The permanent labs will consider transitional job situations >to allow you to work while your license is processed. We also have a number >of temp situations for a great pay scale for techs registered to work in the >state. Travel isn't for everyone, but these are good labs and a great way to >start. > > > > Either way, we'll help you through the process to get you through it as >quickly as possible. If you're even curious, give us a call. Of course we >have a number of other opportunities including sales and management all over >the country. We won't work with every lab--those we place find themselves >in jobs that fit--where they are happy to go to work. We're here to help >and no fee to you. > > > > Tiffany is in the office and I'm traveling as a temp for a couple of >weeks--as always, referral bonuses are available--share us with your >friends! > > > > Tiffany/Office 281.852.9457 > > Cheryl/Cell 281.883.7704 > > or this number follows me around: 800.756.3309 (it will ring a long > > time to find me--please be patient and leave your phone number!) > > > > We return all calls. > > > > Thanks! > > > > Cheryl > > > > > > > > Cheryl Kerry, HT(ASCP) > > Full Staff Inc. > > Staffing the AP Lab by helping one Tech at a time. > > 281.883.7704 c > > 281.852.9457 o > > admin@fullstaff.org > > > > Sign up for the FREE monthly newsletter AP News--updates, tricks of the >trade and current issues for Anatomic Pathology Clinical Labs. Send a >'subscribe' request to APNews@fullstaff.org. Please include your name and >specialty in the body of the email. > > > > ------------------------------ > > > > Message: 10 > > Date: Wed, 2 Aug 2006 09:47:06 +0200 > > From: "louise renton" > > Subject: [Histonet] article from J of histotechnology > > To: Histonet@lists.utsouthwestern.edu > > Message-ID: > > > > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > > > Hi can anyone share a copy of this article? I would be very grateful > > > > "Entering the realm of mineralized bone processing: a review of the > > literature and techniques." J Histotechnol 1997:20 (3) p259-266 > > > > Many thanks > > > > Louise Renton > > Bone Research Unit > > University of the Witwatersrand > > Johannesburg > > South Africa > > "There are nights when the wolves are silent and only the moon howls". > > George Carlin > > No trees were killed in the sending of this message. > > However, many electrons were terribly inconvenienced. > > > > > > > > ------------------------------ > > > > Message: 11 > > Date: Wed, 2 Aug 2006 16:19:02 +0800 > > From: Shirley PHUA > > Subject: [Histonet] Zinc-Based Fixative > > To: histonet@lists.utsouthwestern.edu > > Message-ID: > > > > > g> > > > > Content-Type: text/plain; charset="US-ASCII" > > > > Dear All, > > > > Reference Article: > > Lab Invest 2003 Jun;83(6):889-99 > > Zinc-based fixative improves preservation of genomic DNA and proteins > > in histoprocessing of human tissues > > > > Anyone out there know of the exact composition of any zinc-based > > fixative that is based on the following? > > 1. 0.5% Zinc Chloride and > > 2. 0.5% Zinc Acetate in 0.1M Tris base buffer containing 0.05% calcium > > acetate > > > > > > Many thanks & regards, > > Shirley Phua > > Histopathology Laboratory > > Centre for Forensic Medicine > > Health Sciences Authority > > Singapore > > > > > > > > ------------------------------ > > > > Message: 12 > > Date: Wed, 2 Aug 2006 08:23:30 -0500 > > From: "Vickroy, Jim" > > Subject: [Histonet] prefilled formalin containers > > To: > > Message-ID: > > > > Content-Type: text/plain; charset="us-ascii" > > > > > > > > We have experienced problems with prefilled formalin containers that > > we send to our clients. We have tried two different vendors and they > > both leak. One of the container types claims to have an o-ring type > > lid and the other does not have an o-ring. The containers with the > > o-ring seems to leak as much as the others. In fact the o-ring type > > sometimes leak when they are received from the vendor. If anyone has > > dealt with this successfully please let me know how you solved it. > > > > > > > > Jim Vickroy > > > > Technical Supervisor - Surgical and Autopsy Pathology > > > > Memorial Medical Center > > > > > > > > > > > > This message (including any attachments) contains confidential > > information intended for a specific individual and purpose, and is > > protected by law. If you are not the intended recipient, you should > > delete this message. Any disclosure, copying, or distribution of this >message, or the taking of any action based on it, is strictly prohibited. > > > > > > ------------------------------ > > > > Message: 13 > > Date: Wed, 2 Aug 2006 08:41:32 -0500 > > From: "Jackie M O'Connor" > > Subject: Re: [Histonet] prefilled formalin containers > > To: "Vickroy, Jim" > > Cc: histonet@lists.utsouthwestern.edu, > > histonet-bounces@lists.utsouthwestern.edu > > Message-ID: > > > > > > Content-Type: text/plain; charset="US-ASCII" > > > > Are they leaking on the return trip? Hard to get your clients to make > > sure they are screwed on properly. Each vial/jar should be contained > > within a secondary leakproof bag, anyway - to prevent leaks in transit. > > Give Surgipath a call. > > > > Jackie O' > > > > > > > > "Vickroy, Jim" Sent by: > > histonet-bounces@lists.utsouthwestern.edu > > 08/02/2006 08:23 AM > > > > To > > > > cc > > > > Subject > > [Histonet] prefilled formalin containers > > > > > > > > > > > > > > > > > > We have experienced problems with prefilled formalin containers that > > we send to our clients. We have tried two different vendors and they > > both leak. One of the container types claims to have an o-ring type > > lid and the other does not have an o-ring. The containers with the > > o-ring seems to leak as much as the others. In fact the o-ring type > > sometimes leak when they are received from the vendor. If anyone has > > dealt with this successfully please let me know how you solved it. > > > > > > > > Jim Vickroy > > > > Technical Supervisor - Surgical and Autopsy Pathology > > > > Memorial Medical Center > > > > > > > > > > > > This message (including any attachments) contains confidential > > information intended for a specific individual and purpose, and is > > protected by law. If you are not the intended recipient, you should > > delete this message. Any disclosure, copying, or distribution of this > > message, or the taking of any action based on it, is strictly > > prohibited. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > ------------------------------ > > > > Message: 14 > > Date: Wed, 2 Aug 2006 08:42:33 -0500 > > From: "Ford Royer" > > Subject: [Histonet] '07 TSH Meeting > > To: > > Message-ID: <003101c6b639$82553090$7701a80a@Ford> > > Content-Type: text/plain; charset="us-ascii" > > > > Would the person(s) who are organizing next years Texas Society of > > Histotechnology meeting please contact me? > > > > Thanks! > > > > ~ Ford > > > > Ford M. Royer, MT(ASCP) > > Histology Product Manager > > Minnesota Medical, Inc. > > 7177 Madison Ave. W. > > Golden Valley, MN 55427-3601 > > CELL: 612-839-1046 > > Phone: 763-542-8725 > > Fax: 763-546-4830 > > eMail: froyer@bitstream.net > > > > > > > > > > ------------------------------ > > > > Message: 15 > > Date: Wed, 2 Aug 2006 10:05:40 -0400 > > From: "Santana, Diane" > > Subject: [Histonet] The New VIP > > To: "'histonet@lists.utsouthwestern.edu'" > > > > Message-ID: <4C96AA62BADFD81180CB0002A5AD67FB07113AC5@MAILPMA> > > Content-Type: text/plain; charset="iso-8859-1" > > > > I have a question and please nobody get all defensive about this, but has > > anybody recently bought the VIP 5 tissue processor? If you have, do or did > > you have any problems with it? > > Thanks > > Diane Santana > > PMA > > Haverhill, Mass. > > > > > > > > ------------------------------ > > > > Message: 16 > > Date: Wed, 02 Aug 2006 10:31:26 -0400 > > From: "Fred Underwood" > > Subject: Re: [Histonet] The New VIP > > To: , > > Message-ID: > > Content-Type: text/plain; charset=US-ASCII > > > > I've had mine for a year and (knock, knock on wood) have had no > > problems. > > > > Fred > > > > >>> "Santana, Diane" 08/02 10:05 AM >>> > > I have a question and please nobody get all defensive about this, but > > has > > anybody recently bought the VIP 5 tissue processor? If you have, do or > > did > > you have any problems with it? > > Thanks > > Diane Santana > > PMA > > Haverhill, Mass. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > ------------------------------ > > > > Message: 17 > > Date: Wed, 2 Aug 2006 09:36:48 -0500 > > From: "Henry, Charlene" > > Subject: RE: [Histonet] The New VIP. . > > To: "Santana, Diane" , > > > > Message-ID: > > ><5CB39BCA5724F349BCB748675C6CA1A2099A1D6A@SJMEMXMB02.stjude.sjcrh.local> > > > > Content-Type: text/plain; charset="US-ASCII" > > > > We purchased a VIP5 a few years ago and had several problems so it was > > replaced with a new VIP5. Since that time we have had no problems and it > > has been a reliable instrument. > > Charlene > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Santana, > > Diane > > Sent: Wednesday, August 02, 2006 9:06 AM > > To: 'histonet@lists.utsouthwestern.edu' > > Subject: [Histonet] The New VIP. . > > > > I have a question and please nobody get all defensive about this, but > > has anybody recently bought the VIP 5 tissue processor? If you have, do > > or did you have any problems with it? > > Thanks > > Diane Santana > > PMA > > Haverhill, Mass. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > > ------------------------------ > > > > Message: 18 > > Date: Wed, 2 Aug 2006 10:30:16 -0500 > > From: > > Subject: Re: [Histonet] The New VIP > > To: "Santana, Diane" , > > "'histonet@lists.utsouthwestern.edu'" > > > > Message-ID: > > <20060802153018.PMMJ26069.dukecmmtao03.coxmail.com@dukecmmtao03> > > Content-Type: text/plain; charset=ISO-8859-1 > > > > We have a VIP 5 that we purchased a little over a year ago. Have not had >a single problem with it so far. > > > > Chris Rains > > WPM Pathology Laboratory > > Salina, KS > > > > > > From: "Santana, Diane" > > > Date: 2006/08/02 Wed AM 09:05:40 CDT > > > To: "'histonet@lists.utsouthwestern.edu'" > > > > Subject: [Histonet] The New VIP > > > > > > I have a question and please nobody get all defensive about this, but >has > > > anybody recently bought the VIP 5 tissue processor? If you have, do or >did > > > you have any problems with it? > > > Thanks > > > Diane Santana > > > PMA > > > Haverhill, Mass. > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > Important: This email and any attachments may contain confidential >information subject to protection under the Federal Standards for Privacy of >Individually Identifiable Health Information (45 C.F.R. Parts 160 and 164). >If you or your organization is a "Covered Entity" under the above mentioned >regulations, you are obligated to treat such information in a manner >consistent with the regulations. If it appears that this email was sent to >you in error, (1) you are prohibited from utilizing or disseminating this >email or any attachments; (2) please immediately delete it from your >computer and any servers or other locations where it might be stored and >email (crains@wpmpath.com) or call (Chris Rains) at 785-823-7201 advising >that you have done so. We appreciate your cooperation. > > > > > > > > > > ------------------------------ > > > > Message: 19 > > Date: Wed, 2 Aug 2006 11:42:17 -0400 > > From: "Stahl, Michael" > > Subject: [Histonet] prefilled formalin containers > > To: > > Message-ID: > > <4C878E714B21EB4F8EB159777B8822EE5B6834@bvfyms01.net.bvha.org> > > Content-Type: text/plain; charset="us-ascii" > > > > We use a Surgipath (cat #008100 which are the pre-filled 60ml jars. We > > found these to be almost leak proof. However, they do leak when the jars > > arnt screwed on correctly. But we agree, its not pleasant to have a > > specimen jar in a bio hazard bag full of fixative. Just try to find that > > cervial biospy! > > > > Michael P. Stahl HT (ASCP) > > BVRHC > > 145 W Wallace St > > Findlay, OH 45840 > > mstahl@bhva.org > > > > > > ------------------------------ > > > > Message: 20 > > Date: Wed, 02 Aug 2006 08:55:45 -0700 > > From: Andrea Grantham > > Subject: Re: [Histonet] The New VIP > > To: "'histonet@lists.utsouthwestern.edu'" > > > > Message-ID: > > <4.3.2.7.2.20060802085142.00cec460@algranth.inbox.email.arizona.edu> > > Content-Type: text/plain; charset="us-ascii"; format=flowed > > > > I just got a new VIP 5 and I love it. Wanted one all my (histotech) life > > and I'm so happy to finally have it! It has been great. So easy to use and > > > the tissues are coming out wonderful. Knock-on-wood it will do as well as > > the old Tissue Tek dipper/dunker that it is replacing. > > Andi > > > > > > At 10:30 AM 8/2/2006 -0500, crains@wpmpath.com wrote: > > >We have a VIP 5 that we purchased a little over a year ago. Have not had > > > >a single problem with it so far. > > > > > >Chris Rains > > >WPM Pathology Laboratory > > >Salina, KS > > > > > > > > From: "Santana, Diane" > > > > Date: 2006/08/02 Wed AM 09:05:40 CDT > > > > To: "'histonet@lists.utsouthwestern.edu'" > > > > > > > Subject: [Histonet] The New VIP > > > > > > > > I have a question and please nobody get all defensive about this, but >has > > > > anybody recently bought the VIP 5 tissue processor? If you have, do or >did > > > > you have any problems with it? > > > > Thanks > > > > Diane Santana > > > > PMA > > > > Haverhill, Mass. > > > > > > > > _______________________________________________ > > > > Histonet mailing list > > > > Histonet@lists.utsouthwestern.edu > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > >Important: This email and any attachments may contain confidential > > >information subject to protection under the Federal Standards for Privacy > > > >of Individually Identifiable Health Information (45 C.F.R. Parts 160 and > > >164). If you or your organization is a "Covered Entity" under the above > > >mentioned regulations, you are obligated to treat such information in a > > >manner consistent with the regulations. If it appears that this email >was > > >sent to you in error, (1) you are prohibited from utilizing or > > >disseminating this email or any attachments; (2) please immediately >delete > > >it from your computer and any servers or other locations where it might >be > > >stored and email (crains@wpmpath.com) or call (Chris Rains) at > > >785-823-7201 advising that you have done so. We appreciate your >cooperation. > > > > > > > > >_______________________________________________ > > >Histonet mailing list > > >Histonet@lists.utsouthwestern.edu > > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ..................................................................... > > : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : > > : Sr. Research Specialist University of Arizona : > > : (office: AHSC 4212) P.O. Box 245044 : > > : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : > > : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : > > :...................................................................: > > http://www.cba.arizona.edu/histology-lab.html > > > > > > > > > > ------------------------------ > > > > Message: 21 > > Date: Wed, 2 Aug 2006 11:08:00 -0500 > > From: "Vickroy, Jim" > > Subject: [Histonet] charging and CPT questions > > To: > > Message-ID: > > > > Content-Type: text/plain; charset="us-ascii" > > > > > > > > Can anyone shed any light on the proper way to code and charge the > > following: > > > > > > > > 1. Muscle Biopsy Histochemistry - Frozen sections -88314 or 88319? > > I'm not sure which one. > > > > > > > > 2. Touch preps - Done when we do a frozen section. The frozen > > section is 88331 and the touch prep should be 88333 or 88334? > > > > > > > > 3. Semi-thin sectioning of nerve biopsies - Plastic embedding > > In the past we charged all nerve bxs with an 88348 (EM) charge since > > > > not only did we do the semi-thin sectioning but we > > also did the transmission EM. Our neuropathologist now wants the > > semi-thin sections > > > > routinely but not the rest of the EM examination. > > > > > > > > Thanks > > > > > > > > Jim Vickroy > > > > Technical Supervisor - Surgical and Autopsy Pathology > > > > Memorial Medical Center > > > > > > > > > > > > This message (including any attachments) contains confidential information >intended for a > > specific individual and purpose, and is protected by law. If you are not >the intended recipient, > > you should delete this message. Any disclosure, copying, or distribution >of this message, or the > > taking of any action based on it, is strictly prohibited. > > > > > > ------------------------------ > > > > Message: 22 > > Date: Wed, 2 Aug 2006 09:45:21 -0700 > > From: "Jayne Scoggin" > > Subject: [Histonet] please unsubscribe me from the list > > To: > > Message-ID: <200608021624.k72GO3Vh004624@mx.bioceptlabs.com> > > Content-Type: text/plain; charset="us-ascii" > > > > Please unsubscribe me from the list. > > > > Jayne Scoggin, Biocept Inc. > > 858 320 8224 > > jscoggin@biocept.com > > > > > > > > > > > > ------------------------------ > > > > Message: 23 > > Date: Wed, 2 Aug 2006 12:40:55 -0400 > > From: "Zajic Kari" > > Subject: RE: [Histonet] charging and CPT questions > > To: "Vickroy, Jim" , > > > > Message-ID: > > <095327C7CDBDF64B9E9728A54799091E015CC5A2@ORLEV03.hca.corpad.net> > > Content-Type: text/plain; charset="iso-8859-1" > > > > Hi Jim. I do not do muscle or nerve at my facility but we send to the >University of Miami and they charge the following: > > > > Muscle > > 88305 x1: Surgical Path,gross,micro > > 88313 x2: Paraffin: H&E Plastic > > 88314 x2: cryostat H&E Trichrome > > 88319 x8: Enzyme HistoChem: NADH,Esterase, ATPasex3,Cox, Cox & SDH, SDH > > > > Nerve > > 88305 x1: Surg Path,gross,micro > > 88313 x6: Paraffin H&E,Trichrome,Luxol Fast Blue,Toludine Blue, Crystal >Violet,Silver Stain > > 88314 x2: Cryostat: H&E,Trichrome > > 88319 x1: Frozen Section Histochem: Esterase > > 88348 x1: Nerve Electron Micro > > 88356 x1: Nerve Morphometry > > 88362 x1: Nerve Teased Preps > > > > As far as frozen section touch preps, they came out with new CPT's in 2006 >as follows: > > 88333 Path consult during surgery (cyto exam) initial site ie.touch >prep,squash prep > > physician fee global $75-100+ > > technical $17-25 > > 88334 cyto exam, EACH ADDITIONAL SITE, touch prep,squash prep > > physicain fee $40-50+ > > technical $10-15 > > this info was given to me by Health Systems Concepts,Inc. > > > > hope this helps!!! > > Kari :) > > > > Kari Marie Zajic HT,MLT > > Histology Supervisor > > Palms West Hospital > > Pathology Department > > 13001 State Road Eighty > > Loxahatchee, Florida 33470 > > phone: (561)798-6036 > > telefax: (561)753-4298 > > voicemail: (561)753-4299 > > pager: (561)610-4949 > > email: Kari.Zajic@HCAHealthcare.com > > > > This email and any files transmitted with it may contain PRIVILEGED or >CONFIDENTIAL > > information and may be read or used only by the intended recipient. If you >are not the intended recipient of the email or any of its attachment, please >be advised that you have received this email in error and that any use, >dissemination, distribution, forwarding, printing, or copying of this email >or any attached files is strictly prohibited. If you have received this >email in error, please immediately purge it and all attachments and notify >the sender by reply email or contact the sender at the number listed. > > > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Vickroy, > > Jim > > Sent: Wednesday, August 02, 2006 12:08 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] charging and CPT questions > > > > > > > > > > Can anyone shed any light on the proper way to code and charge the > > following: > > > > > > > > 1. Muscle Biopsy Histochemistry - Frozen sections -88314 or 88319? > > I'm not sure which one. > > > > > > > > 2. Touch preps - Done when we do a frozen section. The frozen > > section is 88331 and the touch prep should be 88333 or 88334? > > > > > > > > 3. Semi-thin sectioning of nerve biopsies - Plastic embedding > > In the past we charged all nerve bxs with an 88348 (EM) charge since > > > > not only did we do the semi-thin sectioning but we > > also did the transmission EM. Our neuropathologist now wants the > > semi-thin sections > > > > routinely but not the rest of the EM examination. > > > > > > > > Thanks > > > > > > > > Jim Vickroy > > > > Technical Supervisor - Surgical and Autopsy Pathology > > > > Memorial Medical Center > > > > > > > > > > > > This message (including any attachments) contains confidential information >intended for a > > specific individual and purpose, and is protected by law. If you are not >the intended recipient, > > you should delete this message. Any disclosure, copying, or distribution >of this message, or the > > taking of any action based on it, is strictly prohibited. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > ------------------------------ > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > End of Histonet Digest, Vol 33, Issue 2 > > *************************************** > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jean.Gillson <@t> elht.nhs.uk Thu Aug 3 10:37:25 2006 From: Jean.Gillson <@t> elht.nhs.uk (Gillson Jean (ELHT) Pathology) Date: Thu Aug 3 10:36:22 2006 Subject: [Histonet] Competency testing Message-ID: <95C57EA9EF5C5A41AC7E075734B75259E42EAB@elht-exch2.xelht.nhs.uk> Hi everyone, In the UK our registration body (HPC) now requires all staff to do CPD (continuing professional development) and prove competency. My question is to what extent? For example: Do you have to prove that they can do every special stain and technique? and on a yearly basis? Can you prove once that you can do all procedures and then just a confirmation on an annual basis without performing everything stain? There are no direct specific guidelines for labs to follow! Any ideas of how other UK labs are dealing with this requirement for HPC and also KSF greatly received. Jean From tpmorken <@t> labvision.com Thu Aug 3 10:41:15 2006 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Thu Aug 3 10:41:29 2006 Subject: [Histonet] ASCP salary survey is deficient Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA2048F7215@usca0082k08.labvision.apogent.com> It seems the ASCP survey is grossly deficient .The recently published survey has woefully incomplete data for the majority of the country. It is so incomplete that they don't even show salary data for lthe majority of the US. : HT only: IL, IN, MI, OH, WI, AR, LA, OK, TX No HT: IA, KS, MN, MO, NE, ND, SD, AK, AZ, CA, CO, HI, ID, MT, NV, NM, OR, UT, WA, WY NO DATA AT ALL!!: AL, DE, CD, FL, GA, KY, MD, MS, NC, SC, TN, VA, WV. In fact, only the North East ( CT, ME, MA, NH, NJ, NY, PS, RI, VT ) has complete data for HT, HTL and HT supervisors. And it is like this for every lab tech group. This doesn't seem like much of a survey to me, despite their claims they made every effort to get data. They got 749 out of 3,544 eligible labs, but obvioulsy those responding were highly concentrated in only a few areas. As such, this survey is nearly useless to most ASCP members. The Advance agazine survey mentioned below is a very different sort of survey - individuals self-responding to a magazine survey. I'm not sure it is much more accurate due to that bias, but it does give very different results. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LuAnn Anderson Sent: Thursday, August 03, 2006 8:07 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Mohs Tech salaries Wow--this survey is way lower than the one Advance conducted last year!! It was published in the 12/7/2005 issue and can be found online. It seems much more realistic, given the current circumstances in the field. The ASCP survey always seems to be on the low side for salaries. I have always questioned who they send the surveys out to--but have not gotten an answer on that as of yet!! At 09:17 PM 8/2/2006, you wrote: >Not specifically for Mohs, but I thought I would let Histonetters know >that the 2005 ASCP Wage and Vacancy survey results are now on the ASCP >BOR >webpage: > >http://www.ascp.org/Certification/ForProgramDirectors/research/default. >aspx > >Click on 2005. > >Survey done Nov. 2005, will be published in the August 2006 "Laboratory >Medicine". Found it on their website last week. > >Peggy A. Wenk, HTL(ASCP)SLS >William Beaumont Hospital >Royal Oak, MI 48073 > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >nsdd114@alltel.net >Sent: Wednesday, August 02, 2006 7:27 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Mohs Tech salaries > >I was wondering if those of you who are doing Mohs would respond to my >question. I am HT-ASCP certified and have 15 years in the field. I am >currently doing Mohs, because the hours and the pay are better in Mohs >in my area. I like doing Mohs and we keep very busy. We started the >lab a year ago and have one Mohs surgeon and are currently doing about >1500 cases a year. Of course that is cases not blocks, because we >frequently do cases with anywhere from 6-25 pieces. Our practice would >like to expand our service and we have tried three techs, all on the >job trained and not certified. I personally know techs who were OJT >trained who are top notch techs. Two of the techs we interviewed >weren't sufficiently skilled to work really independently at high >volume and trouble shoot routines. One didn't know how to tell if she >had a complete skin edge under the scope and she had 3 years of >experience. That's scarey. The 3rd tech is pretty capable, but is >also a CMA and prefers the nursing position. My question is "Does >anyone know of a Mohs salary survey or any guidelines as far as pay >goes for both certified and non-certified techs?" I personally don't >care if someone is confident and capable whether they are certified or >not since it isn't required at this time, but we do want to establish a >pay scale that rewards people based on their overall training and >skill. I do suspect that the next few years will bring some kind of >standardized training requirements and/or certification for Mohs techs. >If anyone has any input I'd appreciate it. We are in the south central east coast area. Thanks, Nancy > > > > From: histonet-request@lists.utsouthwestern.edu > > Date: 2006/08/02 Wed PM 12:01:20 CDT > > To: histonet@lists.utsouthwestern.edu > > Subject: Histonet Digest, Vol 33, Issue 2 > > > > Send Histonet mailing list submissions to > > histonet@lists.utsouthwestern.edu > > > > To subscribe or unsubscribe via the World Wide Web, visit > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > or, via email, send a message with subject or body 'help' to > > histonet-request@lists.utsouthwestern.edu > > > > You can reach the person managing the list at > > histonet-owner@lists.utsouthwestern.edu > > > > When replying, please edit your Subject line so it is more specific > > than "Re: Contents of Histonet digest..." > > > > > > Today's Topics: > > > > 1. RE: Foxp3 on Murine tissue (Melissa Gonzalez) > > 2. RE:BrdU and GFP staining (Pixley, Sarah (pixleysk)) > > 3. Staining cultured cells (Pixley, Sarah (pixleysk)) > > 4. immunohistochemical analysis for connective tissue (Xilong Li) > > 5. ImmunoVision Technologies, Co. (Helen E Johnson) > > 6. Re: ImmunoVision Technologies, Co. (Ron Lagerquist) > > 7. Pennsylvania Histology Meeting for October 2006 (Pamela Marcum) > > 8. RE: Ethanol/other fixations for frozen sections (Tony Henwood) > > 9. Looking for Florida histotechs--or people who are interested > > in Florida relocation: we help with the license. (Cheryl) > > 10. article from J of histotechnology (louise renton) > > 11. Zinc-Based Fixative (Shirley PHUA) > > 12. prefilled formalin containers (Vickroy, Jim) > > 13. Re: prefilled formalin containers (Jackie M O'Connor) > > 14. '07 TSH Meeting (Ford Royer) > > 15. The New VIP (Santana, Diane) > > 16. Re: The New VIP (Fred Underwood) > > 17. RE: The New VIP. . (Henry, Charlene) > > 18. Re: The New VIP (crains@wpmpath.com) > > 19. prefilled formalin containers (Stahl, Michael) > > 20. Re: The New VIP (Andrea Grantham) > > 21. charging and CPT questions (Vickroy, Jim) > > 22. please unsubscribe me from the list (Jayne Scoggin) > > 23. RE: charging and CPT questions (Zajic Kari) > > > > > > -------------------------------------------------------------------- > > -- > > > > Message: 1 > > Date: Tue, 1 Aug 2006 10:18:52 -0700 > > From: "Melissa Gonzalez" > > Subject: [Histonet] RE: Foxp3 on Murine tissue > > To: > > Message-ID: > > > > Content-Type: text/plain; charset="us-ascii" > > > > I've also tried several of the products out there with no success on > > murine tissues as well. I would be interested in hearing if anyone > > has had success with FoxP3 yet on murine tissues, and what you could > > use as a positive control. > > > > Thanks, > > Melissa > > ------------------------------ > > > > Message: 7 > > Date: Mon, 31 Jul 2006 14:02:03 -0500 > > From: "Drew Allan Roenneburg" > > Subject: [Histonet] Foxp3 on Murine tissue > > To: > > Message-ID: <44CE0D5B0200009C00001F23@gw.surgery.wisc.edu> > > Content-Type: text/plain; charset=US-ASCII > > > > Hi, I was wondering if anyone has stained for foxp3 on frozen or > > FFPE mouse tissues (spleen). I have tried the Abcam rabbit > > anti-human foxp3 antibody that works on FFPE human and monkey > > (tonsil and spleen), however have had little success on mouse. > > Thanks Drew Roenneburg > > > > > > > > ------------------------------ > > > > Message: 2 > > Date: Tue, 1 Aug 2006 13:45:30 -0400 > > From: "Pixley, Sarah \(pixleysk\)" > > Subject: [Histonet] RE:BrdU and GFP staining > > To: > > Message-ID: > > > > Content-Type: text/plain; charset="us-ascii" > > > > Dear Steve: > > I would stain for GFP with an antibody to GFP and do labeling with > > DAB. I recommend the Elite ABC kits. The DAB is moderately resistant > > to the BrdU penetration protocols. Then, fix your tissues well with > > paraformaldehyde, i.e. 15 mins, 4%, and proceed with the BrdU, using > > a system that labels with a fluorescent antibody. This will only > > work, however, if your GFP is in different cells, or it is in the > > cytoplasm of large cells that have no nuclear staining. The DAB can > > obscure the secondary fluorescent labeling. > > > > Good luck. Stay Cool back at you. > > Sarah Pixley, > > Ohio > > > > Message: 2 > > Date: Mon, 31 Jul 2006 10:22:41 -0700 (PDT) > > From: Steven Coakley > > Subject: [Histonet] BrdU and GFP expression > > To: Histonet@lists.utsouthwestern.edu > > Message-ID: <20060731172241.65699.qmail@web38215.mail.mud.yahoo.com> > > Content-Type: text/plain; charset=iso-8859-1 > > > > Good afternoon from WI, > > > > We have several FFPE blocks from 2003 that express GFP. Our lead > > scientist would like to perform Brdu IHC in the same slides hoping > > to express both. As expected the AR removes the GFP green label. > > Has anyone any experience expressing Brdu and GFP on the same slide. > > > > Stay cool, > > > > Steve > > > > > > > > > > > > ------------------------------ > > > > Message: 3 > > Date: Tue, 1 Aug 2006 13:49:31 -0400 > > From: "Pixley, Sarah \(pixleysk\)" > > Subject: [Histonet] Staining cultured cells > > To: > > Message-ID: > > > > Content-Type: text/plain; charset="us-ascii" > > > > > > To stain cultured cells we routinely fix with 4% paraformaldehyde > > for 15-20 mins. But if the antigen is sensitive to aldehydes, then > > we fix with ice-cold 100% methanol for 5-10 mins. Your acetone > > technique for 30 mins is extremely harsh for cultured cells. If you > > want to continue with it, I would fix with the acetone for only a > > few minutes and try that. > > > > As for the other person who asked about staining cultured cells, we > > do exactly the same ICC as on sections, although we usually are able > > to dilute the primary antibodies more than with frozen tissue > > sections (using paraformaldehyde perfused tissues). > > > > Sarah Pixley > > Ohio > > > > > > Message: 4 > > Date: Mon, 31 Jul 2006 13:41:54 -0400 > > From: Christopher C Overend > > > > Subject: [Histonet] IFA staining problems > > To: histonet@lists.utsouthwestern.edu > > Message-ID: <1fbaf901fb5b0e.1fb5b0e1fbaf90@huskymail.uconn.edu> > > Content-Type: text/plain; charset=us-ascii > > > > I am new to the technique of immunofluorescence, and have been doing > > some work with it lately on cultured cells. Specifically, I have > > been staining intracellular virus with very good results. However, > > when i try to detect cellular proteins, I do not get any staining. > > The proceedure i have been using consists of "fixation" with 90% > > acetone for 30 min, at 4degrees C. the rest of the process had been > > much like an ELISA, all incubations have been for 30 min at > > 4degrees. The buffer i have been using is PBS, 1%BSA, 0.1%NaN3. > > Someone mentioned the problem could be from the acetone and > > suggested trying a glyoxal fixative. Can anyone offer some insights, > > or if there is a different protocol i might have to follow using a > > glyoxal fixative with tissue >culture? > > Thank you! > > Chris > > > > Christopher Overend > > Ph.D Student > > University of Connecticut > > Department of Pathobiology and Veterinary Sciences Storrs, CT > > Christopher.overend@uconn.edu > > > > > > > > > > > > ------------------------------ > > > > Message: 4 > > Date: Tue, 01 Aug 2006 13:23:06 -0500 > > From: "Xilong Li" > > Subject: [Histonet] immunohistochemical analysis for connective > > tissue > > To: > > Message-ID: <44CF55BA020000F000004E06@swnw124.swmed.edu> > > Content-Type: text/plain; charset=US-ASCII > > > > Hi, All members, > > > > I am looking for a consistent protocol to stain collagen of > > connective tissues in muscle frozen sections by immunohistochemical > > analysis including information for primary antibody and second > > antibody used in the protocols. > > > > Thanks in advance. > > > > xilong li > > > > Dr. Xilong Li > > Hypertension Division, Internal Medicine University of Texas > > Southwestern Medical Center 5323 Harry Hiness Blvd-J4.142 > > Dallas, TX 75390 > > > > Tel: 214-648-9966(L) > > Fax: 214-648-7902 > > > > > > > > > > ------------------------------ > > > > Message: 5 > > Date: Tue, 1 Aug 2006 14:24:37 -0400 > > From: Helen E Johnson > > Subject: [Histonet] ImmunoVision Technologies, Co. > > To: histonet@lists.utsouthwestern.edu > > Message-ID: > > > > > lt > > h.state.ny.us> > > > > Content-Type: text/plain; charset=US-ASCII > > > > > > Hi Histonetters, > > Does anyone know if ImmunoVision Technologies, Co. is still in > > business? No one answers the phone ( 650-992-7563). > > Helen > > (hej01@health.state.ny.us) > > > > > > > > > > ------------------------------ > > > > Message: 6 > > Date: Tue, 1 Aug 2006 11:32:41 -0700 (PDT) > > From: Ron Lagerquist > > Subject: Re: [Histonet] ImmunoVision Technologies, Co. > > To: Helen E Johnson , > > histonet@lists.utsouthwestern.edu > > Message-ID: <20060801183241.80617.qmail@web56106.mail.re3.yahoo.com> > > Content-Type: text/plain; charset=iso-8859-1 > > > > They were purchased by VisionBiosystems (800-753-7264) > > http://www.vision-bio.com/media_releases.html > > > > Ron > > > > Helen E Johnson wrote: > > > > Hi Histonetters, > > Does anyone know if ImmunoVision Technologies, Co. is still in > > business? No one answers the phone ( 650-992-7563). Helen > > (hej01@health.state.ny.us) > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > --------------------------------- > > Do you Yahoo!? > > Next-gen email? Have it all with the all-new Yahoo! Mail Beta. > > > > ------------------------------ > > > > Message: 7 > > Date: Tue, 01 Aug 2006 14:33:51 -0400 > > From: Pamela Marcum > > Subject: [Histonet] Pennsylvania Histology Meeting for October 2006 > > To: histonet@lists.utsouthwestern.edu > > Message-ID: <6.1.1.1.2.20060801142646.01a26d88@mail.vet.upenn.edu> > > Content-Type: text/plain; charset="us-ascii"; format=flowed > > > > > > > > Good Afternoon All, > > > > We have our web site up and partially up. We do have our program > > online and have the program in the download file area for you to > > review and if register. The program can be printed off. Just go to > > www.pahisto.org and look for downloads in the top line. Click on > > and open or save. The format is a Microsoft Word document. If you > > have a problem or would need a pre-printing copy please let either > > me or Gloria Limetti know. Gloria can be reached at > > glorialimetti@yahoo.com or >412-647-8535. > > > > Thanks for your patience and we will be updating the program with > > vendors and other information as we near the meeting in October. > > > > > > Best Regards, > > > > Pamela A Marcum > > Manager, Histology Special Procedures > > University of Pennsylvania > > School of Veterinary Medicine > > R.S. Reynolds Jr. CORL > > New Bolton Center > > 382 West Street Road > > Kennett Square, PA 19348 > > > > Phone - 610-925-6278 > > Fax - 610-925-8120 > > E-mail - pmarcum@vet.upenn.edu > > > > > > > > > > > > ------------------------------ > > > > Message: 8 > > Date: Wed, 2 Aug 2006 10:49:46 +1000 > > From: "Tony Henwood" > > Subject: RE: [Histonet] Ethanol/other fixations for frozen sections > > To: "Andrea T. Hooper" , "Histonet" > > > > Message-ID: > > > > Content-Type: text/plain; charset="us-ascii" > > > > Urgent Frozen Sections for H&E - we fix in methanol > > > > Regards > > > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory > > Manager & Senior Scientist The Children's Hospital at Westmead, > > Locked Bag 4001, Westmead, 2145, AUSTRALIA. > > Tel: 612 9845 3306 > > Fax: 612 9845 3318 > > > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > > Andrea T. Hooper > > Sent: Monday, 31 July 2006 10:45 PM > > To: Histonet > > Subject: [Histonet] Ethanol/other fixations for frozen sections > > > > > > Just taking a survey .... What are everyone's thoughts on using > > ethanol for frozen sections? I have a colleague who swears by it but > > I am an acetone person myself. > > > > Thoughts, comments, input on what is the best fix for frozen section > > IHC? > > > > Thanks! > > -- > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ******************************************************************** > > ** > > This email and any files transmitted with it are confidential and intended >solely for the use of the individual or entity to whom they are >addressed. If you are not the intended recipient, please delete it and >notify the sender. > > > > Views expressed in this message and any attachments are those of the > > individual sender, and are not necessarily the views of The > > Children's Hospital at Westmead > > > > This note also confirms that this email message has been virus > > scanned and although no computer viruses were detected, the > > Childrens Hospital at >Westmead accepts no liability for any consequential damage resulting >from email containing computer viruses. > > ******************************************************************** > > ** > > > > > > > > > > ------------------------------ > > > > Message: 9 > > Date: Tue, 1 Aug 2006 19:40:34 -0700 (PDT) > > From: Cheryl > > Subject: [Histonet] Looking for Florida histotechs--or people who are > > interested in Florida relocation: we help with the license. > > To: Histonet > > Message-ID: <20060802024034.14424.qmail@web50904.mail.yahoo.com> > > Content-Type: text/plain; charset=iso-8859-1 > > > > Hello everyone-- > > > > Florida's registry process has created a major shortage of > > qualified >techs in the state. We have a number of Florida permanent and temp >opportunities. The permanent labs will consider transitional job >situations to allow you to work while your license is processed. We >also have a number of temp situations for a great pay scale for techs >registered to work in the state. Travel isn't for everyone, but these >are good labs and a great way to start. > > > > Either way, we'll help you through the process to get you through > > it as >quickly as possible. If you're even curious, give us a call. Of >course we have a number of other opportunities including sales and >management all over the country. We won't work with every lab--those >we place find themselves in jobs that fit--where they are happy to go >to work. We're here to help and no fee to you. > > > > Tiffany is in the office and I'm traveling as a temp for a couple > > of >weeks--as always, referral bonuses are available--share us with your >friends! > > > > Tiffany/Office 281.852.9457 > > Cheryl/Cell 281.883.7704 > > or this number follows me around: 800.756.3309 (it will ring a > > long time to find me--please be patient and leave your phone > > number!) > > > > We return all calls. > > > > Thanks! > > > > Cheryl > > > > > > > > Cheryl Kerry, HT(ASCP) > > Full Staff Inc. > > Staffing the AP Lab by helping one Tech at a time. 281.883.7704 c > > 281.852.9457 o > > admin@fullstaff.org > > > > Sign up for the FREE monthly newsletter AP News--updates, tricks of > > the >trade and current issues for Anatomic Pathology Clinical Labs. Send a >'subscribe' request to APNews@fullstaff.org. Please include your name >and specialty in the body of the email. > > > > ------------------------------ > > > > Message: 10 > > Date: Wed, 2 Aug 2006 09:47:06 +0200 > > From: "louise renton" > > Subject: [Histonet] article from J of histotechnology > > To: Histonet@lists.utsouthwestern.edu > > Message-ID: > > > > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > > > Hi can anyone share a copy of this article? I would be very grateful > > > > "Entering the realm of mineralized bone processing: a review of the > > literature and techniques." J Histotechnol 1997:20 (3) p259-266 > > > > Many thanks > > > > Louise Renton > > Bone Research Unit > > University of the Witwatersrand > > Johannesburg > > South Africa > > "There are nights when the wolves are silent and only the moon > > howls". George Carlin No trees were killed in the sending of this > > message. However, many electrons were terribly inconvenienced. > > > > > > > > ------------------------------ > > > > Message: 11 > > Date: Wed, 2 Aug 2006 16:19:02 +0800 > > From: Shirley PHUA > > Subject: [Histonet] Zinc-Based Fixative > > To: histonet@lists.utsouthwestern.edu > > Message-ID: > > > > > .s > > g> > > > > Content-Type: text/plain; charset="US-ASCII" > > > > Dear All, > > > > Reference Article: > > Lab Invest 2003 Jun;83(6):889-99 > > Zinc-based fixative improves preservation of genomic DNA and > > proteins in histoprocessing of human tissues > > > > Anyone out there know of the exact composition of any zinc-based > > fixative that is based on the following? 1. 0.5% Zinc Chloride and > > 2. 0.5% Zinc Acetate in 0.1M Tris base buffer containing 0.05% calcium > > acetate > > > > > > Many thanks & regards, > > Shirley Phua > > Histopathology Laboratory > > Centre for Forensic Medicine > > Health Sciences Authority > > Singapore > > > > > > > > ------------------------------ > > > > Message: 12 > > Date: Wed, 2 Aug 2006 08:23:30 -0500 > > From: "Vickroy, Jim" > > Subject: [Histonet] prefilled formalin containers > > To: > > Message-ID: > > > > Content-Type: text/plain; charset="us-ascii" > > > > > > > > We have experienced problems with prefilled formalin containers that > > we send to our clients. We have tried two different vendors and > > they both leak. One of the container types claims to have an o-ring > > type lid and the other does not have an o-ring. The containers > > with the o-ring seems to leak as much as the others. In fact the > > o-ring type sometimes leak when they are received from the vendor. > > If anyone has dealt with this successfully please let me know how > > you solved it. > > > > > > > > Jim Vickroy > > > > Technical Supervisor - Surgical and Autopsy Pathology > > > > Memorial Medical Center > > > > > > > > > > > > This message (including any attachments) contains confidential > > information intended for a specific individual and purpose, and is > > protected by law. If you are not the intended recipient, you should > > delete this message. Any disclosure, copying, or distribution of > > this >message, or the taking of any action based on it, is strictly >prohibited. > > > > > > ------------------------------ > > > > Message: 13 > > Date: Wed, 2 Aug 2006 08:41:32 -0500 > > From: "Jackie M O'Connor" > > Subject: Re: [Histonet] prefilled formalin containers > > To: "Vickroy, Jim" > > Cc: histonet@lists.utsouthwestern.edu, > > histonet-bounces@lists.utsouthwestern.edu > > Message-ID: > > > > > m> > > Content-Type: text/plain; charset="US-ASCII" > > > > Are they leaking on the return trip? Hard to get your clients to > > make sure they are screwed on properly. Each vial/jar should be > > contained within a secondary leakproof bag, anyway - to prevent > > leaks in transit. Give Surgipath a call. > > > > Jackie O' > > > > > > > > "Vickroy, Jim" Sent by: > > histonet-bounces@lists.utsouthwestern.edu > > 08/02/2006 08:23 AM > > > > To > > > > cc > > > > Subject > > [Histonet] prefilled formalin containers > > > > > > > > > > > > > > > > > > We have experienced problems with prefilled formalin containers that > > we send to our clients. We have tried two different vendors and > > they both leak. One of the container types claims to have an o-ring > > type lid and the other does not have an o-ring. The containers > > with the o-ring seems to leak as much as the others. In fact the > > o-ring type sometimes leak when they are received from the vendor. > > If anyone has dealt with this successfully please let me know how > > you solved it. > > > > > > > > Jim Vickroy > > > > Technical Supervisor - Surgical and Autopsy Pathology > > > > Memorial Medical Center > > > > > > > > > > > > This message (including any attachments) contains confidential > > information intended for a specific individual and purpose, and is > > protected by law. If you are not the intended recipient, you should > > delete this message. Any disclosure, copying, or distribution of > > this message, or the taking of any action based on it, is strictly > > prohibited. _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > ------------------------------ > > > > Message: 14 > > Date: Wed, 2 Aug 2006 08:42:33 -0500 > > From: "Ford Royer" > > Subject: [Histonet] '07 TSH Meeting > > To: > > Message-ID: <003101c6b639$82553090$7701a80a@Ford> > > Content-Type: text/plain; charset="us-ascii" > > > > Would the person(s) who are organizing next years Texas Society of > > Histotechnology meeting please contact me? > > > > Thanks! > > > > ~ Ford > > > > Ford M. Royer, MT(ASCP) > > Histology Product Manager > > Minnesota Medical, Inc. > > 7177 Madison Ave. W. > > Golden Valley, MN 55427-3601 > > CELL: 612-839-1046 > > Phone: 763-542-8725 > > Fax: 763-546-4830 > > eMail: froyer@bitstream.net > > > > > > > > > > ------------------------------ > > > > Message: 15 > > Date: Wed, 2 Aug 2006 10:05:40 -0400 > > From: "Santana, Diane" > > Subject: [Histonet] The New VIP > > To: "'histonet@lists.utsouthwestern.edu'" > > > > Message-ID: <4C96AA62BADFD81180CB0002A5AD67FB07113AC5@MAILPMA> > > Content-Type: text/plain; charset="iso-8859-1" > > > > I have a question and please nobody get all defensive about this, > > but has anybody recently bought the VIP 5 tissue processor? If you > > have, do or did you have any problems with it? Thanks > > Diane Santana > > PMA > > Haverhill, Mass. > > > > > > > > ------------------------------ > > > > Message: 16 > > Date: Wed, 02 Aug 2006 10:31:26 -0400 > > From: "Fred Underwood" > > Subject: Re: [Histonet] The New VIP > > To: , > > Message-ID: > > Content-Type: text/plain; charset=US-ASCII > > > > I've had mine for a year and (knock, knock on wood) have had no > > problems. > > > > Fred > > > > >>> "Santana, Diane" 08/02 10:05 AM >>> > > I have a question and please nobody get all defensive about this, > > but has anybody recently bought the VIP 5 tissue processor? If you > > have, do or did > > you have any problems with it? > > Thanks > > Diane Santana > > PMA > > Haverhill, Mass. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > ------------------------------ > > > > Message: 17 > > Date: Wed, 2 Aug 2006 09:36:48 -0500 > > From: "Henry, Charlene" > > Subject: RE: [Histonet] The New VIP. . > > To: "Santana, Diane" , > > > > Message-ID: > > ><5CB39BCA5724F349BCB748675C6CA1A2099A1D6A@SJMEMXMB02.stjude.sjcrh.local >> > > > > Content-Type: text/plain; charset="US-ASCII" > > > > We purchased a VIP5 a few years ago and had several problems so it > > was replaced with a new VIP5. Since that time we have had no > > problems and it has been a reliable instrument. Charlene > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > > Santana, Diane > > Sent: Wednesday, August 02, 2006 9:06 AM > > To: 'histonet@lists.utsouthwestern.edu' > > Subject: [Histonet] The New VIP. . > > > > I have a question and please nobody get all defensive about this, > > but has anybody recently bought the VIP 5 tissue processor? If you > > have, do or did you have any problems with it? Thanks > > Diane Santana > > PMA > > Haverhill, Mass. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > > ------------------------------ > > > > Message: 18 > > Date: Wed, 2 Aug 2006 10:30:16 -0500 > > From: > > Subject: Re: [Histonet] The New VIP > > To: "Santana, Diane" , > > "'histonet@lists.utsouthwestern.edu'" > > > > Message-ID: > > > > <20060802153018.PMMJ26069.dukecmmtao03.coxmail.com@dukecmmtao03> > > Content-Type: text/plain; charset=ISO-8859-1 > > > > We have a VIP 5 that we purchased a little over a year ago. Have > > not had >a single problem with it so far. > > > > Chris Rains > > WPM Pathology Laboratory > > Salina, KS > > > > > > From: "Santana, Diane" > > > Date: 2006/08/02 Wed AM 09:05:40 CDT > > > To: "'histonet@lists.utsouthwestern.edu'" > > > > Subject: [Histonet] The New VIP > > > > > > I have a question and please nobody get all defensive about this, > > > but >has > > > anybody recently bought the VIP 5 tissue processor? If you have, > > > do or >did > > > you have any problems with it? > > > Thanks > > > Diane Santana > > > PMA > > > Haverhill, Mass. > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > Important: This email and any attachments may contain confidential >information subject to protection under the Federal Standards for >Privacy of Individually Identifiable Health Information (45 C.F.R. >Parts 160 and 164). If you or your organization is a "Covered Entity" >under the above mentioned regulations, you are obligated to treat such >information in a manner consistent with the regulations. If it appears >that this email was sent to you in error, (1) you are prohibited from >utilizing or disseminating this email or any attachments; (2) please >immediately delete it from your computer and any servers or other >locations where it might be stored and email (crains@wpmpath.com) or >call (Chris Rains) at 785-823-7201 advising that you have done so. We >appreciate your cooperation. > > > > > > > > > > ------------------------------ > > > > Message: 19 > > Date: Wed, 2 Aug 2006 11:42:17 -0400 > > From: "Stahl, Michael" > > Subject: [Histonet] prefilled formalin containers > > To: > > Message-ID: > > <4C878E714B21EB4F8EB159777B8822EE5B6834@bvfyms01.net.bvha.org> > > Content-Type: text/plain; charset="us-ascii" > > > > We use a Surgipath (cat #008100 which are the pre-filled 60ml jars. > > We found these to be almost leak proof. However, they do leak when > > the jars arnt screwed on correctly. But we agree, its not pleasant > > to have a specimen jar in a bio hazard bag full of fixative. Just > > try to find that cervial biospy! > > > > Michael P. Stahl HT (ASCP) > > BVRHC > > 145 W Wallace St > > Findlay, OH 45840 > > mstahl@bhva.org > > > > > > ------------------------------ > > > > Message: 20 > > Date: Wed, 02 Aug 2006 08:55:45 -0700 > > From: Andrea Grantham > > Subject: Re: [Histonet] The New VIP > > To: "'histonet@lists.utsouthwestern.edu'" > > > > Message-ID: > > > > <4.3.2.7.2.20060802085142.00cec460@algranth.inbox.email.arizona.edu> > > Content-Type: text/plain; charset="us-ascii"; format=flowed > > > > I just got a new VIP 5 and I love it. Wanted one all my (histotech) > > life and I'm so happy to finally have it! It has been great. So easy > > to use and > > > the tissues are coming out wonderful. Knock-on-wood it will do as > > well as the old Tissue Tek dipper/dunker that it is replacing. Andi > > > > > > At 10:30 AM 8/2/2006 -0500, crains@wpmpath.com wrote: > > >We have a VIP 5 that we purchased a little over a year ago. Have > > >not had > > > >a single problem with it so far. > > > > > >Chris Rains > > >WPM Pathology Laboratory > > >Salina, KS > > > > > > > > From: "Santana, Diane" > > > > Date: 2006/08/02 Wed AM 09:05:40 CDT > > > > To: "'histonet@lists.utsouthwestern.edu'" > > > > > > > Subject: [Histonet] The New VIP > > > > > > > > I have a question and please nobody get all defensive about > > > > this, but >has > > > > anybody recently bought the VIP 5 tissue processor? If you have, > > > > do or >did > > > > you have any problems with it? > > > > Thanks > > > > Diane Santana > > > > PMA > > > > Haverhill, Mass. > > > > > > > > _______________________________________________ > > > > Histonet mailing list > > > > Histonet@lists.utsouthwestern.edu > > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > >Important: This email and any attachments may contain confidential > > >information subject to protection under the Federal Standards for > > >Privacy > > > >of Individually Identifiable Health Information (45 C.F.R. Parts > > >160 and 164). If you or your organization is a "Covered Entity" > > >under the above mentioned regulations, you are obligated to treat > > >such information in a manner consistent with the regulations. If > > >it appears that this email >was > > >sent to you in error, (1) you are prohibited from utilizing or > > >disseminating this email or any attachments; (2) please immediately >delete > > >it from your computer and any servers or other locations where it > > >might >be > > >stored and email (crains@wpmpath.com) or call (Chris Rains) at > > >785-823-7201 advising that you have done so. We appreciate your >cooperation. > > > > > > > > >_______________________________________________ > > >Histonet mailing list > > >Histonet@lists.utsouthwestern.edu > > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ..................................................................... > > : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : > > : Sr. Research Specialist University of Arizona : > > : (office: AHSC 4212) P.O. Box 245044 : > > : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : > > : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : > > :...................................................................: > > http://www.cba.arizona.edu/histology-lab.html > > > > > > > > > > ------------------------------ > > > > Message: 21 > > Date: Wed, 2 Aug 2006 11:08:00 -0500 > > From: "Vickroy, Jim" > > Subject: [Histonet] charging and CPT questions > > To: > > Message-ID: > > > > Content-Type: text/plain; charset="us-ascii" > > > > > > > > Can anyone shed any light on the proper way to code and charge the > > following: > > > > > > > > 1. Muscle Biopsy Histochemistry - Frozen sections -88314 or 88319? > > I'm not sure which one. > > > > > > > > 2. Touch preps - Done when we do a frozen section. The frozen > > section is 88331 and the touch prep should be 88333 or 88334? > > > > > > > > 3. Semi-thin sectioning of nerve biopsies - Plastic embedding > > In the past we charged all nerve bxs with an 88348 (EM) charge since > > > > not only did we do the semi-thin sectioning but > > we also did the transmission EM. Our neuropathologist now wants > > the semi-thin sections > > > > routinely but not the rest of the EM > > examination. > > > > > > > > Thanks > > > > > > > > Jim Vickroy > > > > Technical Supervisor - Surgical and Autopsy Pathology > > > > Memorial Medical Center > > > > > > > > > > > > This message (including any attachments) contains confidential > > information >intended for a > > specific individual and purpose, and is protected by law. If you are > > not >the intended recipient, > > you should delete this message. Any disclosure, copying, or > > distribution >of this message, or the > > taking of any action based on it, is strictly prohibited. > > > > > > ------------------------------ > > > > Message: 22 > > Date: Wed, 2 Aug 2006 09:45:21 -0700 > > From: "Jayne Scoggin" > > Subject: [Histonet] please unsubscribe me from the list > > To: > > Message-ID: <200608021624.k72GO3Vh004624@mx.bioceptlabs.com> > > Content-Type: text/plain; charset="us-ascii" > > > > Please unsubscribe me from the list. > > > > Jayne Scoggin, Biocept Inc. > > 858 320 8224 > > jscoggin@biocept.com > > > > > > > > > > > > ------------------------------ > > > > Message: 23 > > Date: Wed, 2 Aug 2006 12:40:55 -0400 > > From: "Zajic Kari" > > Subject: RE: [Histonet] charging and CPT questions > > To: "Vickroy, Jim" , > > > > Message-ID: > > <095327C7CDBDF64B9E9728A54799091E015CC5A2@ORLEV03.hca.corpad.net> > > Content-Type: text/plain; charset="iso-8859-1" > > > > Hi Jim. I do not do muscle or nerve at my facility but we send to > > the >University of Miami and they charge the following: > > > > Muscle > > 88305 x1: Surgical Path,gross,micro > > 88313 x2: Paraffin: H&E Plastic > > 88314 x2: cryostat H&E Trichrome > > 88319 x8: Enzyme HistoChem: NADH,Esterase, ATPasex3,Cox, Cox & SDH, > > SDH > > > > Nerve > > 88305 x1: Surg Path,gross,micro > > 88313 x6: Paraffin H&E,Trichrome,Luxol Fast Blue,Toludine Blue, > > Crystal >Violet,Silver Stain > > 88314 x2: Cryostat: H&E,Trichrome > > 88319 x1: Frozen Section Histochem: Esterase > > 88348 x1: Nerve Electron Micro > > 88356 x1: Nerve Morphometry > > 88362 x1: Nerve Teased Preps > > > > As far as frozen section touch preps, they came out with new CPT's > > in 2006 >as follows: > > 88333 Path consult during surgery (cyto exam) initial site ie.touch >prep,squash prep > > physician fee global $75-100+ > > technical $17-25 > > 88334 cyto exam, EACH ADDITIONAL SITE, touch prep,squash prep > > physicain fee $40-50+ > > technical $10-15 > > this info was given to me by Health Systems Concepts,Inc. > > > > hope this helps!!! > > Kari :) > > > > Kari Marie Zajic HT,MLT > > Histology Supervisor > > Palms West Hospital > > Pathology Department > > 13001 State Road Eighty > > Loxahatchee, Florida 33470 > > phone: (561)798-6036 > > telefax: (561)753-4298 > > voicemail: (561)753-4299 > > pager: (561)610-4949 > > email: Kari.Zajic@HCAHealthcare.com > > > > This email and any files transmitted with it may contain PRIVILEGED > > or >CONFIDENTIAL > > information and may be read or used only by the intended recipient. > > If you >are not the intended recipient of the email or any of its attachment, >please be advised that you have received this email in error and that >any use, dissemination, distribution, forwarding, printing, or copying >of this email or any attached files is strictly prohibited. If you have >received this email in error, please immediately purge it and all >attachments and notify the sender by reply email or contact the sender >at the number listed. > > > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of > > Vickroy, Jim > > Sent: Wednesday, August 02, 2006 12:08 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] charging and CPT questions > > > > > > > > > > Can anyone shed any light on the proper way to code and charge the > > following: > > > > > > > > 1. Muscle Biopsy Histochemistry - Frozen sections -88314 or 88319? > > I'm not sure which one. > > > > > > > > 2. Touch preps - Done when we do a frozen section. The frozen > > section is 88331 and the touch prep should be 88333 or 88334? > > > > > > > > 3. Semi-thin sectioning of nerve biopsies - Plastic embedding > > In the past we charged all nerve bxs with an 88348 (EM) charge since > > > > not only did we do the semi-thin sectioning but > > we also did the transmission EM. Our neuropathologist now wants > > the semi-thin sections > > > > routinely but not the rest of the EM > > examination. > > > > > > > > Thanks > > > > > > > > Jim Vickroy > > > > Technical Supervisor - Surgical and Autopsy Pathology > > > > Memorial Medical Center > > > > > > > > > > > > This message (including any attachments) contains confidential > > information >intended for a > > specific individual and purpose, and is protected by law. If you are > > not >the intended recipient, > > you should delete this message. Any disclosure, copying, or > > distribution >of this message, or the > > taking of any action based on it, is strictly prohibited. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > ------------------------------ > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > End of Histonet Digest, Vol 33, Issue 2 > > *************************************** > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From mgr <@t> seattleskincancer.com Thu Aug 3 10:59:42 2006 From: mgr <@t> seattleskincancer.com (Office Manager) Date: Thu Aug 3 10:59:51 2006 Subject: [Histonet] Mohs Tech salaries In-Reply-To: <20060802232644.FGSK28894.ispmxmta05-srv.windstream.net@webmail-relay.alltel.net> Message-ID: <001d01c6b715$d5b31f40$2500000a@corp.birkby.com> You can call the American Society for Mohs Histotechnology at 414-347-1103. They do a salary and benefit survey every year or two. Beth Seattle Skin Cancer Center -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of nsdd114@alltel.net Sent: Wednesday, August 02, 2006 4:27 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mohs Tech salaries I was wondering if those of you who are doing Mohs would respond to my question. I am HT-ASCP certified and have 15 years in the field. I am currently doing Mohs, because the hours and the pay are better in Mohs in my area. I like doing Mohs and we keep very busy. We started the lab a year ago and have one Mohs surgeon and are currently doing about 1500 cases a year. Of course that is cases not blocks, because we frequently do cases with anywhere from 6-25 pieces. Our practice would like to expand our service and we have tried three techs, all on the job trained and not certified. I personally know techs who were OJT trained who are top notch techs. Two of the techs we interviewed weren't sufficiently skilled to work really independently at high volume and trouble shoot routines. One didn't know how to tell if she had a complete skin edge under the scope and she had 3 years of experience. That's scarey. The 3rd tech is pretty capable, but is also a CMA and prefers the nursing position. My question is "Does anyone know of a Mohs salary survey or any guidelines as far as pay goes for both certified and non-certified techs?" I personally don't care if someone is confident and capable whether they are certified or not since it isn't required at this time, but we do want to establish a pay scale that rewards people based on their overall training and skill. I do suspect that the next few years will bring some kind of standardized training requirements and/or certification for Mohs techs. If anyone has any input I'd appreciate it. We are in the south central east coast area. Thanks, Nancy > > From: histonet-request@lists.utsouthwestern.edu > Date: 2006/08/02 Wed PM 12:01:20 CDT > To: histonet@lists.utsouthwestern.edu > Subject: Histonet Digest, Vol 33, Issue 2 > > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. RE: Foxp3 on Murine tissue (Melissa Gonzalez) > 2. RE:BrdU and GFP staining (Pixley, Sarah (pixleysk)) > 3. Staining cultured cells (Pixley, Sarah (pixleysk)) > 4. immunohistochemical analysis for connective tissue (Xilong Li) > 5. ImmunoVision Technologies, Co. (Helen E Johnson) > 6. Re: ImmunoVision Technologies, Co. (Ron Lagerquist) > 7. Pennsylvania Histology Meeting for October 2006 (Pamela Marcum) > 8. RE: Ethanol/other fixations for frozen sections (Tony Henwood) > 9. Looking for Florida histotechs--or people who are interested > in Florida relocation: we help with the license. (Cheryl) > 10. article from J of histotechnology (louise renton) > 11. Zinc-Based Fixative (Shirley PHUA) > 12. prefilled formalin containers (Vickroy, Jim) > 13. Re: prefilled formalin containers (Jackie M O'Connor) > 14. '07 TSH Meeting (Ford Royer) > 15. The New VIP (Santana, Diane) > 16. Re: The New VIP (Fred Underwood) > 17. RE: The New VIP. . (Henry, Charlene) > 18. Re: The New VIP (crains@wpmpath.com) > 19. prefilled formalin containers (Stahl, Michael) > 20. Re: The New VIP (Andrea Grantham) > 21. charging and CPT questions (Vickroy, Jim) > 22. please unsubscribe me from the list (Jayne Scoggin) > 23. RE: charging and CPT questions (Zajic Kari) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 1 Aug 2006 10:18:52 -0700 > From: "Melissa Gonzalez" > Subject: [Histonet] RE: Foxp3 on Murine tissue > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > I've also tried several of the products out there with no success on > murine tissues as well. I would be interested in hearing if anyone has > had success with FoxP3 yet on murine tissues, and what you could use as > a positive control. > > Thanks, > Melissa > ------------------------------ > > Message: 7 > Date: Mon, 31 Jul 2006 14:02:03 -0500 > From: "Drew Allan Roenneburg" > Subject: [Histonet] Foxp3 on Murine tissue > To: > Message-ID: <44CE0D5B0200009C00001F23@gw.surgery.wisc.edu> > Content-Type: text/plain; charset=US-ASCII > > Hi, I was wondering if anyone has stained for foxp3 on frozen or FFPE > mouse tissues (spleen). I have tried the Abcam rabbit anti-human foxp3 > antibody that works on FFPE human and monkey (tonsil and spleen), > however have had little success on mouse. Thanks > Drew Roenneburg > > > > ------------------------------ > > Message: 2 > Date: Tue, 1 Aug 2006 13:45:30 -0400 > From: "Pixley, Sarah \(pixleysk\)" > Subject: [Histonet] RE:BrdU and GFP staining > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > Dear Steve: > I would stain for GFP with an antibody to GFP and do labeling with DAB. > I recommend the Elite ABC kits. The DAB is moderately resistant to the > BrdU penetration protocols. Then, fix your tissues well with > paraformaldehyde, i.e. 15 mins, 4%, and proceed with the BrdU, using a > system that labels with a fluorescent antibody. This will only work, > however, if your GFP is in different cells, or it is in the cytoplasm of > large cells that have no nuclear staining. The DAB can obscure the > secondary fluorescent labeling. > > Good luck. Stay Cool back at you. > Sarah Pixley, > Ohio > > Message: 2 > Date: Mon, 31 Jul 2006 10:22:41 -0700 (PDT) > From: Steven Coakley > Subject: [Histonet] BrdU and GFP expression > To: Histonet@lists.utsouthwestern.edu > Message-ID: <20060731172241.65699.qmail@web38215.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Good afternoon from WI, > > We have several FFPE blocks from 2003 that express GFP. Our lead > scientist would like to perform Brdu IHC in the same slides hoping to > express both. As expected the AR removes the GFP green label. Has > anyone any experience expressing Brdu and GFP on the same slide. > > Stay cool, > > Steve > > > > > > ------------------------------ > > Message: 3 > Date: Tue, 1 Aug 2006 13:49:31 -0400 > From: "Pixley, Sarah \(pixleysk\)" > Subject: [Histonet] Staining cultured cells > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > > To stain cultured cells we routinely fix with 4% paraformaldehyde for > 15-20 mins. But if the antigen is sensitive to aldehydes, then we fix > with ice-cold 100% methanol for 5-10 mins. Your acetone technique for 30 > mins is extremely harsh for cultured cells. If you want to continue with > it, I would fix with the acetone for only a few minutes and try that. > > As for the other person who asked about staining cultured cells, we do > exactly the same ICC as on sections, although we usually are able to > dilute the primary antibodies more than with frozen tissue sections > (using paraformaldehyde perfused tissues). > > Sarah Pixley > Ohio > > > Message: 4 > Date: Mon, 31 Jul 2006 13:41:54 -0400 > From: Christopher C Overend > Subject: [Histonet] IFA staining problems > To: histonet@lists.utsouthwestern.edu > Message-ID: <1fbaf901fb5b0e.1fb5b0e1fbaf90@huskymail.uconn.edu> > Content-Type: text/plain; charset=us-ascii > > I am new to the technique of immunofluorescence, and have been doing > some work with it lately on cultured cells. Specifically, I have been > staining intracellular virus with very good results. However, when i try > to detect cellular proteins, I do not get any staining. The proceedure i > have been using consists of "fixation" with 90% acetone for 30 min, at > 4degrees C. the rest of the process had been much like an ELISA, all > incubations have been for 30 min at 4degrees. The buffer i have been > using is PBS, 1%BSA, 0.1%NaN3. Someone mentioned the problem could be > from the acetone and suggested trying a glyoxal fixative. Can anyone > offer some insights, or if there is a different protocol i might have to > follow using a glyoxal fixative with tissue culture? > Thank you! > Chris > > Christopher Overend > Ph.D Student > University of Connecticut > Department of Pathobiology and Veterinary Sciences Storrs, CT > Christopher.overend@uconn.edu > > > > > > ------------------------------ > > Message: 4 > Date: Tue, 01 Aug 2006 13:23:06 -0500 > From: "Xilong Li" > Subject: [Histonet] immunohistochemical analysis for connective tissue > To: > Message-ID: <44CF55BA020000F000004E06@swnw124.swmed.edu> > Content-Type: text/plain; charset=US-ASCII > > Hi, All members, > > I am looking for a consistent protocol to stain collagen of connective > tissues in muscle frozen sections by immunohistochemical analysis > including information for primary antibody and second antibody used in > the protocols. > > Thanks in advance. > > xilong li > > Dr. Xilong Li > Hypertension Division, Internal Medicine > University of Texas Southwestern Medical Center > 5323 Harry Hiness Blvd-J4.142 > Dallas, TX 75390 > > Tel: 214-648-9966(L) > Fax: 214-648-7902 > > > > > ------------------------------ > > Message: 5 > Date: Tue, 1 Aug 2006 14:24:37 -0400 > From: Helen E Johnson > Subject: [Histonet] ImmunoVision Technologies, Co. > To: histonet@lists.utsouthwestern.edu > Message-ID: > > > Content-Type: text/plain; charset=US-ASCII > > > Hi Histonetters, > Does anyone know if ImmunoVision Technologies, Co. is still in > business? No one answers the phone ( 650-992-7563). > Helen > (hej01@health.state.ny.us) > > > > > ------------------------------ > > Message: 6 > Date: Tue, 1 Aug 2006 11:32:41 -0700 (PDT) > From: Ron Lagerquist > Subject: Re: [Histonet] ImmunoVision Technologies, Co. > To: Helen E Johnson , > histonet@lists.utsouthwestern.edu > Message-ID: <20060801183241.80617.qmail@web56106.mail.re3.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > They were purchased by VisionBiosystems (800-753-7264) > http://www.vision-bio.com/media_releases.html > > Ron > > Helen E Johnson wrote: > > Hi Histonetters, > Does anyone know if ImmunoVision Technologies, Co. is still in > business? No one answers the phone ( 650-992-7563). > Helen > (hej01@health.state.ny.us) > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > --------------------------------- > Do you Yahoo!? > Next-gen email? Have it all with the all-new Yahoo! Mail Beta. > > ------------------------------ > > Message: 7 > Date: Tue, 01 Aug 2006 14:33:51 -0400 > From: Pamela Marcum > Subject: [Histonet] Pennsylvania Histology Meeting for October 2006 > To: histonet@lists.utsouthwestern.edu > Message-ID: <6.1.1.1.2.20060801142646.01a26d88@mail.vet.upenn.edu> > Content-Type: text/plain; charset="us-ascii"; format=flowed > > > > Good Afternoon All, > > We have our web site up and partially up. We do have our program online > and have the program in the download file area for you to review and if > register. The program can be printed off. Just go to www.pahisto.org and > look for downloads in the top line. Click on and open or save. The format > is a Microsoft Word document. If you have a problem or would need a > pre-printing copy please let either me or Gloria Limetti know. Gloria can > be reached at glorialimetti@yahoo.com or 412-647-8535. > > Thanks for your patience and we will be updating the program with vendors > and other information as we near the meeting in October. > > > Best Regards, > > Pamela A Marcum > Manager, Histology Special Procedures > University of Pennsylvania > School of Veterinary Medicine > R.S. Reynolds Jr. CORL > New Bolton Center > 382 West Street Road > Kennett Square, PA 19348 > > Phone - 610-925-6278 > Fax - 610-925-8120 > E-mail - pmarcum@vet.upenn.edu > > > > > > ------------------------------ > > Message: 8 > Date: Wed, 2 Aug 2006 10:49:46 +1000 > From: "Tony Henwood" > Subject: RE: [Histonet] Ethanol/other fixations for frozen sections > To: "Andrea T. Hooper" , "Histonet" > > Message-ID: > Content-Type: text/plain; charset="us-ascii" > > Urgent Frozen Sections for H&E - we fix in methanol > > Regards > > Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) > Laboratory Manager & Senior Scientist > The Children's Hospital at Westmead, > Locked Bag 4001, Westmead, 2145, AUSTRALIA. > Tel: 612 9845 3306 > Fax: 612 9845 3318 > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Andrea > T. Hooper > Sent: Monday, 31 July 2006 10:45 PM > To: Histonet > Subject: [Histonet] Ethanol/other fixations for frozen sections > > > Just taking a survey .... What are everyone's thoughts on using > ethanol for frozen sections? I have a colleague who swears by it but > I am an acetone person myself. > > Thoughts, comments, input on what is the best fix for frozen section > IHC? > > Thanks! > -- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ********************************************************************** > This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. > > Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead > > This note also confirms that this email message has been > virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. > ********************************************************************** > > > > > ------------------------------ > > Message: 9 > Date: Tue, 1 Aug 2006 19:40:34 -0700 (PDT) > From: Cheryl > Subject: [Histonet] Looking for Florida histotechs--or people who are > interested in Florida relocation: we help with the license. > To: Histonet > Message-ID: <20060802024034.14424.qmail@web50904.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Hello everyone-- > > Florida's registry process has created a major shortage of qualified techs in the state. We have a number of Florida permanent and temp opportunities. The permanent labs will consider transitional job situations to allow you to work while your license is processed. We also have a number of temp situations for a great pay scale for techs registered to work in the state. Travel isn't for everyone, but these are good labs and a great way to start. > > Either way, we'll help you through the process to get you through it as quickly as possible. If you're even curious, give us a call. Of course we have a number of other opportunities including sales and management all over the country. We won't work with every lab--those we place find themselves in jobs that fit--where they are happy to go to work. We're here to help and no fee to you. > > Tiffany is in the office and I'm traveling as a temp for a couple of weeks--as always, referral bonuses are available--share us with your friends! > > Tiffany/Office 281.852.9457 > Cheryl/Cell 281.883.7704 > or this number follows me around: 800.756.3309 (it will ring a long time to find me--please be patient and leave your phone number!) > > We return all calls. > > Thanks! > > Cheryl > > > > Cheryl Kerry, HT(ASCP) > Full Staff Inc. > Staffing the AP Lab by helping one Tech at a time. > 281.883.7704 c > 281.852.9457 o > admin@fullstaff.org > > Sign up for the FREE monthly newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email. > > ------------------------------ > > Message: 10 > Date: Wed, 2 Aug 2006 09:47:06 +0200 > From: "louise renton" > Subject: [Histonet] article from J of histotechnology > To: Histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Hi can anyone share a copy of this article? I would be very grateful > > "Entering the realm of mineralized bone processing: a review of the > literature and techniques." J Histotechnol 1997:20 (3) p259-266 > > Many thanks > > Louise Renton > Bone Research Unit > University of the Witwatersrand > Johannesburg > South Africa > "There are nights when the wolves are silent and only the moon howls". > George Carlin > No trees were killed in the sending of this message. > However, many electrons were terribly inconvenienced. > > > > ------------------------------ > > Message: 11 > Date: Wed, 2 Aug 2006 16:19:02 +0800 > From: Shirley PHUA > Subject: [Histonet] Zinc-Based Fixative > To: histonet@lists.utsouthwestern.edu > Message-ID: > > > Content-Type: text/plain; charset="US-ASCII" > > Dear All, > > Reference Article: > Lab Invest 2003 Jun;83(6):889-99 > Zinc-based fixative improves preservation of genomic DNA and proteins in > histoprocessing of human tissues > > Anyone out there know of the exact composition of any zinc-based fixative > that is based on the following? > 1. 0.5% Zinc Chloride and > 2. 0.5% Zinc Acetate in 0.1M Tris base buffer containing 0.05% calcium > acetate > > > Many thanks & regards, > Shirley Phua > Histopathology Laboratory > Centre for Forensic Medicine > Health Sciences Authority > Singapore > > > > ------------------------------ > > Message: 12 > Date: Wed, 2 Aug 2006 08:23:30 -0500 > From: "Vickroy, Jim" > Subject: [Histonet] prefilled formalin containers > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > > > We have experienced problems with prefilled formalin containers that we > send to our clients. We have tried two different vendors and they both > leak. One of the container types claims to have an o-ring type lid and > the other does not have an o-ring. The containers with the o-ring seems > to leak as much as the others. In fact the o-ring type sometimes leak > when they are received from the vendor. If anyone has dealt with this > successfully please let me know how you solved it. > > > > Jim Vickroy > > Technical Supervisor - Surgical and Autopsy Pathology > > Memorial Medical Center > > > > > > This message (including any attachments) contains confidential information intended for a > specific individual and purpose, and is protected by law. If you are not the intended recipient, > you should delete this message. Any disclosure, copying, or distribution of this message, or the > taking of any action based on it, is strictly prohibited. > > > ------------------------------ > > Message: 13 > Date: Wed, 2 Aug 2006 08:41:32 -0500 > From: "Jackie M O'Connor" > Subject: Re: [Histonet] prefilled formalin containers > To: "Vickroy, Jim" > Cc: histonet@lists.utsouthwestern.edu, > histonet-bounces@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > Are they leaking on the return trip? Hard to get your clients to make > sure they are screwed on properly. Each vial/jar should be contained > within a secondary leakproof bag, anyway - to prevent leaks in transit. > Give Surgipath a call. > > Jackie O' > > > > "Vickroy, Jim" > Sent by: histonet-bounces@lists.utsouthwestern.edu > 08/02/2006 08:23 AM > > To > > cc > > Subject > [Histonet] prefilled formalin containers > > > > > > > > > We have experienced problems with prefilled formalin containers that we > send to our clients. We have tried two different vendors and they both > leak. One of the container types claims to have an o-ring type lid and > the other does not have an o-ring. The containers with the o-ring seems > to leak as much as the others. In fact the o-ring type sometimes leak > when they are received from the vendor. If anyone has dealt with this > successfully please let me know how you solved it. > > > > Jim Vickroy > > Technical Supervisor - Surgical and Autopsy Pathology > > Memorial Medical Center > > > > > > This message (including any attachments) contains confidential information > intended for a > specific individual and purpose, and is protected by law. If you are not > the intended recipient, > you should delete this message. Any disclosure, copying, or distribution > of this message, or the > taking of any action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 14 > Date: Wed, 2 Aug 2006 08:42:33 -0500 > From: "Ford Royer" > Subject: [Histonet] '07 TSH Meeting > To: > Message-ID: <003101c6b639$82553090$7701a80a@Ford> > Content-Type: text/plain; charset="us-ascii" > > Would the person(s) who are organizing next years Texas Society of > Histotechnology meeting please contact me? > > Thanks! > > ~ Ford > > Ford M. Royer, MT(ASCP) > Histology Product Manager > Minnesota Medical, Inc. > 7177 Madison Ave. W. > Golden Valley, MN 55427-3601 > CELL: 612-839-1046 > Phone: 763-542-8725 > Fax: 763-546-4830 > eMail: froyer@bitstream.net > > > > > ------------------------------ > > Message: 15 > Date: Wed, 2 Aug 2006 10:05:40 -0400 > From: "Santana, Diane" > Subject: [Histonet] The New VIP > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: <4C96AA62BADFD81180CB0002A5AD67FB07113AC5@MAILPMA> > Content-Type: text/plain; charset="iso-8859-1" > > I have a question and please nobody get all defensive about this, but has > anybody recently bought the VIP 5 tissue processor? If you have, do or did > you have any problems with it? > Thanks > Diane Santana > PMA > Haverhill, Mass. > > > > ------------------------------ > > Message: 16 > Date: Wed, 02 Aug 2006 10:31:26 -0400 > From: "Fred Underwood" > Subject: Re: [Histonet] The New VIP > To: , > Message-ID: > Content-Type: text/plain; charset=US-ASCII > > I've had mine for a year and (knock, knock on wood) have had no > problems. > > Fred > > >>> "Santana, Diane" 08/02 10:05 AM >>> > I have a question and please nobody get all defensive about this, but > has > anybody recently bought the VIP 5 tissue processor? If you have, do or > did > you have any problems with it? > Thanks > Diane Santana > PMA > Haverhill, Mass. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 17 > Date: Wed, 2 Aug 2006 09:36:48 -0500 > From: "Henry, Charlene" > Subject: RE: [Histonet] The New VIP. . > To: "Santana, Diane" , > > Message-ID: > <5CB39BCA5724F349BCB748675C6CA1A2099A1D6A@SJMEMXMB02.stjude.sjcrh.local> > > Content-Type: text/plain; charset="US-ASCII" > > We purchased a VIP5 a few years ago and had several problems so it was > replaced with a new VIP5. Since that time we have had no problems and it > has been a reliable instrument. > Charlene > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Santana, > Diane > Sent: Wednesday, August 02, 2006 9:06 AM > To: 'histonet@lists.utsouthwestern.edu' > Subject: [Histonet] The New VIP. . > > I have a question and please nobody get all defensive about this, but > has anybody recently bought the VIP 5 tissue processor? If you have, do > or did you have any problems with it? > Thanks > Diane Santana > PMA > Haverhill, Mass. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 18 > Date: Wed, 2 Aug 2006 10:30:16 -0500 > From: > Subject: Re: [Histonet] The New VIP > To: "Santana, Diane" , > "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > <20060802153018.PMMJ26069.dukecmmtao03.coxmail.com@dukecmmtao03> > Content-Type: text/plain; charset=ISO-8859-1 > > We have a VIP 5 that we purchased a little over a year ago. Have not had a single problem with it so far. > > Chris Rains > WPM Pathology Laboratory > Salina, KS > > > > From: "Santana, Diane" > > Date: 2006/08/02 Wed AM 09:05:40 CDT > > To: "'histonet@lists.utsouthwestern.edu'" > > Subject: [Histonet] The New VIP > > > > I have a question and please nobody get all defensive about this, but has > > anybody recently bought the VIP 5 tissue processor? If you have, do or did > > you have any problems with it? > > Thanks > > Diane Santana > > PMA > > Haverhill, Mass. > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > Important: This email and any attachments may contain confidential information subject to protection under the Federal Standards for Privacy of Individually Identifiable Health Information (45 C.F.R. Parts 160 and 164). If you or your organization is a "Covered Entity" under the above mentioned regulations, you are obligated to treat such information in a manner consistent with the regulations. If it appears that this email was sent to you in error, (1) you are prohibited from utilizing or disseminating this email or any attachments; (2) please immediately delete it from your computer and any servers or other locations where it might be stored and email (crains@wpmpath.com) or call (Chris Rains) at 785-823-7201 advising that you have done so. We appreciate your cooperation. > > > > > ------------------------------ > > Message: 19 > Date: Wed, 2 Aug 2006 11:42:17 -0400 > From: "Stahl, Michael" > Subject: [Histonet] prefilled formalin containers > To: > Message-ID: > <4C878E714B21EB4F8EB159777B8822EE5B6834@bvfyms01.net.bvha.org> > Content-Type: text/plain; charset="us-ascii" > > We use a Surgipath (cat #008100 which are the pre-filled 60ml jars. We > found these to be almost leak proof. However, they do leak when the jars > arnt screwed on correctly. But we agree, its not pleasant to have a > specimen jar in a bio hazard bag full of fixative. Just try to find that > cervial biospy! > > Michael P. Stahl HT (ASCP) > BVRHC > 145 W Wallace St > Findlay, OH 45840 > mstahl@bhva.org > > > ------------------------------ > > Message: 20 > Date: Wed, 02 Aug 2006 08:55:45 -0700 > From: Andrea Grantham > Subject: Re: [Histonet] The New VIP > To: "'histonet@lists.utsouthwestern.edu'" > > Message-ID: > <4.3.2.7.2.20060802085142.00cec460@algranth.inbox.email.arizona.edu> > Content-Type: text/plain; charset="us-ascii"; format=flowed > > I just got a new VIP 5 and I love it. Wanted one all my (histotech) life > and I'm so happy to finally have it! It has been great. So easy to use and > the tissues are coming out wonderful. Knock-on-wood it will do as well as > the old Tissue Tek dipper/dunker that it is replacing. > Andi > > > At 10:30 AM 8/2/2006 -0500, crains@wpmpath.com wrote: > >We have a VIP 5 that we purchased a little over a year ago. Have not had > >a single problem with it so far. > > > >Chris Rains > >WPM Pathology Laboratory > >Salina, KS > > > > > > From: "Santana, Diane" > > > Date: 2006/08/02 Wed AM 09:05:40 CDT > > > To: "'histonet@lists.utsouthwestern.edu'" > > > > > Subject: [Histonet] The New VIP > > > > > > I have a question and please nobody get all defensive about this, but has > > > anybody recently bought the VIP 5 tissue processor? If you have, do or did > > > you have any problems with it? > > > Thanks > > > Diane Santana > > > PMA > > > Haverhill, Mass. > > > > > > _______________________________________________ > > > Histonet mailing list > > > Histonet@lists.utsouthwestern.edu > > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > >Important: This email and any attachments may contain confidential > >information subject to protection under the Federal Standards for Privacy > >of Individually Identifiable Health Information (45 C.F.R. Parts 160 and > >164). If you or your organization is a "Covered Entity" under the above > >mentioned regulations, you are obligated to treat such information in a > >manner consistent with the regulations. If it appears that this email was > >sent to you in error, (1) you are prohibited from utilizing or > >disseminating this email or any attachments; (2) please immediately delete > >it from your computer and any servers or other locations where it might be > >stored and email (crains@wpmpath.com) or call (Chris Rains) at > >785-823-7201 advising that you have done so. We appreciate your cooperation. > > > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ..................................................................... > : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : > : Sr. Research Specialist University of Arizona : > : (office: AHSC 4212) P.O. Box 245044 : > : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : > : (FAX: 520-626-2097) (email: algranth@u.arizona.edu) : > :...................................................................: > http://www.cba.arizona.edu/histology-lab.html > > > > > ------------------------------ > > Message: 21 > Date: Wed, 2 Aug 2006 11:08:00 -0500 > From: "Vickroy, Jim" > Subject: [Histonet] charging and CPT questions > To: > Message-ID: > > Content-Type: text/plain; charset="us-ascii" > > > > Can anyone shed any light on the proper way to code and charge the > following: > > > > 1. Muscle Biopsy Histochemistry - Frozen sections -88314 or 88319? > I'm not sure which one. > > > > 2. Touch preps - Done when we do a frozen section. The frozen > section is 88331 and the touch prep should be 88333 or 88334? > > > > 3. Semi-thin sectioning of nerve biopsies - Plastic embedding > In the past we charged all nerve bxs with an 88348 (EM) charge since > > not only did we do the semi-thin sectioning but we > also did the transmission EM. Our neuropathologist now wants the > semi-thin sections > > routinely but not the rest of the EM examination. > > > > Thanks > > > > Jim Vickroy > > Technical Supervisor - Surgical and Autopsy Pathology > > Memorial Medical Center > > > > > > This message (including any attachments) contains confidential information intended for a > specific individual and purpose, and is protected by law. If you are not the intended recipient, > you should delete this message. Any disclosure, copying, or distribution of this message, or the > taking of any action based on it, is strictly prohibited. > > > ------------------------------ > > Message: 22 > Date: Wed, 2 Aug 2006 09:45:21 -0700 > From: "Jayne Scoggin" > Subject: [Histonet] please unsubscribe me from the list > To: > Message-ID: <200608021624.k72GO3Vh004624@mx.bioceptlabs.com> > Content-Type: text/plain; charset="us-ascii" > > Please unsubscribe me from the list. > > Jayne Scoggin, Biocept Inc. > 858 320 8224 > jscoggin@biocept.com > > > > > > ------------------------------ > > Message: 23 > Date: Wed, 2 Aug 2006 12:40:55 -0400 > From: "Zajic Kari" > Subject: RE: [Histonet] charging and CPT questions > To: "Vickroy, Jim" , > > Message-ID: > <095327C7CDBDF64B9E9728A54799091E015CC5A2@ORLEV03.hca.corpad.net> > Content-Type: text/plain; charset="iso-8859-1" > > Hi Jim. I do not do muscle or nerve at my facility but we send to the University of Miami and they charge the following: > > Muscle > 88305 x1: Surgical Path,gross,micro > 88313 x2: Paraffin: H&E Plastic > 88314 x2: cryostat H&E Trichrome > 88319 x8: Enzyme HistoChem: NADH,Esterase, ATPasex3,Cox, Cox & SDH, SDH > > Nerve > 88305 x1: Surg Path,gross,micro > 88313 x6: Paraffin H&E,Trichrome,Luxol Fast Blue,Toludine Blue, Crystal Violet,Silver Stain > 88314 x2: Cryostat: H&E,Trichrome > 88319 x1: Frozen Section Histochem: Esterase > 88348 x1: Nerve Electron Micro > 88356 x1: Nerve Morphometry > 88362 x1: Nerve Teased Preps > > As far as frozen section touch preps, they came out with new CPT's in 2006 as follows: > 88333 Path consult during surgery (cyto exam) initial site ie.touch prep,squash prep > physician fee global $75-100+ > technical $17-25 > 88334 cyto exam, EACH ADDITIONAL SITE, touch prep,squash prep > physicain fee $40-50+ > technical $10-15 > this info was given to me by Health Systems Concepts,Inc. > > hope this helps!!! > Kari :) > > Kari Marie Zajic HT,MLT > Histology Supervisor > Palms West Hospital > Pathology Department > 13001 State Road Eighty > Loxahatchee, Florida 33470 > phone: (561)798-6036 > telefax: (561)753-4298 > voicemail: (561)753-4299 > pager: (561)610-4949 > email: Kari.Zajic@HCAHealthcare.com > > This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL > information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Vickroy, > Jim > Sent: Wednesday, August 02, 2006 12:08 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] charging and CPT questions > > > > > Can anyone shed any light on the proper way to code and charge the > following: > > > > 1. Muscle Biopsy Histochemistry - Frozen sections -88314 or 88319? > I'm not sure which one. > > > > 2. Touch preps - Done when we do a frozen section. The frozen > section is 88331 and the touch prep should be 88333 or 88334? > > > > 3. Semi-thin sectioning of nerve biopsies - Plastic embedding > In the past we charged all nerve bxs with an 88348 (EM) charge since > > not only did we do the semi-thin sectioning but we > also did the transmission EM. Our neuropathologist now wants the > semi-thin sections > > routinely but not the rest of the EM examination. > > > > Thanks > > > > Jim Vickroy > > Technical Supervisor - Surgical and Autopsy Pathology > > Memorial Medical Center > > > > > > This message (including any attachments) contains confidential information intended for a > specific individual and purpose, and is protected by law. If you are not the intended recipient, > you should delete this message. Any disclosure, copying, or distribution of this message, or the > taking of any action based on it, is strictly prohibited. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 33, Issue 2 > *************************************** > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JonesLy <@t> mir.wustl.edu Thu Aug 3 11:08:35 2006 From: JonesLy <@t> mir.wustl.edu (JonesLy@mir.wustl.edu) Date: Thu Aug 3 11:08:44 2006 Subject: [Histonet] Please edit Digest replies In-Reply-To: <20060803153413.7563570935@expurgate2.wustl.edu> Message-ID: Please, please, pretty please delete the complete digest(s) when replying to the list. Just include the one message to which you are responding. I think I've seen three digest replies just this morning. Thanks in advance from me (and our e-mail administrators who keep reminding me that my mail file has again exceeded quota). Lynne Jones From SHARON.OSBORN <@t> SPCORP.COM Thu Aug 3 11:35:01 2006 From: SHARON.OSBORN <@t> SPCORP.COM (Osborn, Sharon) Date: Thu Aug 3 11:35:08 2006 Subject: [Histonet] Cryostats Message-ID: <9A919A5D70313A4D9C56A025710874080C7365@kenmsg40.us.schp.com> Melissa, We have several Leica CM3050-S model cryostats. Since I have been at one almost exclusively for the past month and will be for at least another month, I can say that I love it. They are very reliable, comfortable to work with (sittng on a stool or chair) and easy to manuever around in. The sales rep is very knowledgeable for he was previously a service technician for Leica. The service technician is one of the best--knowledgeable and personable. In fact, all of the Leica people who have been in to do service have been excellent. And, the staff coordinating the service are very good. Your rep is same as ours: Paul Raimondo phone 415.359.6461. Call him for information. Should you want to see these, come on down (call to set a time) or talk with Paul about arranging a visit to see one in operation, try out, etc. Sharon Osborn DNAX, Schering Plough BioPharma Palo Alto, Ca 650.496.6539 Message: 3 Date: Wed, 2 Aug 2006 09:48:36 -0700 From: "Melissa Gonzalez" Subject: [Histonet] Opinions on cryostats please.. To: Message-ID: Content-Type: text/plain; charset="us-ascii" We are looking into budgeting a used/new cryostat for next year, and I was wondering how much other labs have spent on theirs, and how happy they are with the equipment, reliability, service, etc. It's been awhile since I've looked at units and am curious if there are any popular pieces out there right now. Thanks a lot for the input, and feel free to respond off-line. Vendor responses welcome. Melissa Melissa Gonzalez Cell Genesys, Inc. South San Francisco, CA 94080 ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From GDawson <@t> dynacaremilwaukee.com Thu Aug 3 11:38:57 2006 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Thu Aug 3 11:39:02 2006 Subject: [Histonet] ASCP salary survey is deficient Message-ID: All, The ASCP survey has become a tool for management to justify low histotech pay scales. I have learned this from personal experience. Even when the survey covers your region, the numbers do NOT jive with the reality of what it takes to actually hire a histotech. Since this has been the case for as long as I've been checking this survey, I would strongly suggest that each laboratory base offers hinged on what other labs in the area are offering and don't bring the ASCP survey into play at all. My Opinion Only, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI From wellborj <@t> mercyhealth.com Thu Aug 3 11:47:56 2006 From: wellborj <@t> mercyhealth.com (Janci Wellborn) Date: Thu Aug 3 11:48:27 2006 Subject: [Histonet] Just wondering if anyone out there has a procedure on making your own control tissue for organisms? Message-ID: Just wondering if anyone out there has a procedure on making your own control tissue for organisms? Any help in this area would be appreciated. Thank-you, Janci Wellborn, BS, BSeD, HTL Dunes Medical Lab Dakota Dunes, SD From tpmorken <@t> labvision.com Thu Aug 3 12:09:34 2006 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Thu Aug 3 12:09:46 2006 Subject: [Histonet] ASCP salary survey is deficient Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA2048F7218@usca0082k08.labvision.apogent.com> Glen, Yes I think you are right. Managers have almost no incentive to respond to the survey at all, much less with real figures. They most likely give a mid-range figure for a given position since the mid-range is usually what they will offer prospective employees with extensive experience. Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Thursday, August 03, 2006 9:39 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] ASCP salary survey is deficient All, The ASCP survey has become a tool for management to justify low histotech pay scales. I have learned this from personal experience. Even when the survey covers your region, the numbers do NOT jive with the reality of what it takes to actually hire a histotech. Since this has been the case for as long as I've been checking this survey, I would strongly suggest that each laboratory base offers hinged on what other labs in the area are offering and don't bring the ASCP survey into play at all. My Opinion Only, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AGrobe2555 <@t> aol.com Thu Aug 3 12:18:17 2006 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Thu Aug 3 12:18:31 2006 Subject: [Histonet] Endothelial cell antibodies Message-ID: <244.611daf80.320389d9@aol.com> Michele, I have used an anti-VWF on paraffin and cryostat sections as well as eNOS abs. The VWF was a polyclonal, and consequently had a bit more background, but the ECs were stained very nicely. The argument for the VWF, is that not all ECs may express VWF. eNOS should be present in ECs. Albert Albert C. Grobe, PhD International Heart Institute, Tissue Engineering Lab Saint Patrick Hospital 554 W. Broadway Missoula, MT 59802 (406) 329-5634 Lab (406) 329-5880 Fax From DOOLEEO <@t> shands.ufl.edu Thu Aug 3 12:57:13 2006 From: DOOLEEO <@t> shands.ufl.edu (Elaine Dooley) Date: Thu Aug 3 12:57:46 2006 Subject: [Histonet] MUM1 Message-ID: Dear Histonetters, I am getting ready to purchase an antibody to MUM1 Protein. I noticed that they suggest 1mMEDTA at 9.0ph for antigen retrieval. Has anyone tried Ventana CC1 for antigen retrieval? Any suggestions of dilutions and pretreatments would be appreciated. Elaine Dooley HTL Shands Teaching Hosp. 352-265-0111 ext 72117 From Barry.R.Rittman <@t> uth.tmc.edu Thu Aug 3 13:01:48 2006 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Thu Aug 3 13:01:55 2006 Subject: [Histonet] MUM1 Message-ID: Elaine Haven't heard of this protein before. Does it operate against birth mothers, mother in laws or mothers in general? Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elaine Dooley Sent: Thursday, August 03, 2006 12:57 PM To: Histonet@pathology.swmed.edu Subject: [Histonet] MUM1 Dear Histonetters, I am getting ready to purchase an antibody to MUM1 Protein. I noticed that they suggest 1mMEDTA at 9.0ph for antigen retrieval. Has anyone tried Ventana CC1 for antigen retrieval? Any suggestions of dilutions and pretreatments would be appreciated. Elaine Dooley HTL Shands Teaching Hosp. 352-265-0111 ext 72117 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Thu Aug 3 13:19:58 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Aug 3 13:20:33 2006 Subject: [Histonet] MUM1 In-Reply-To: Message-ID: Biocare has a concentrated EDTA solution that you mix 1:4 with distilled water. I've had good consistent results with it. It beats making the stuff up from scratch. Jackie O' "Elaine Dooley" Sent by: histonet-bounces@lists.utsouthwestern.edu 08/03/2006 12:57 PM To cc Subject [Histonet] MUM1 Dear Histonetters, I am getting ready to purchase an antibody to MUM1 Protein. I noticed that they suggest 1mMEDTA at 9.0ph for antigen retrieval. Has anyone tried Ventana CC1 for antigen retrieval? Any suggestions of dilutions and pretreatments would be appreciated. Elaine Dooley HTL Shands Teaching Hosp. 352-265-0111 ext 72117 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Thu Aug 3 13:20:50 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Thu Aug 3 13:21:20 2006 Subject: [Histonet] MUM1 In-Reply-To: Message-ID: No one is allowed to say - - they're mum. "Rittman, Barry R" Sent by: histonet-bounces@lists.utsouthwestern.edu 08/03/2006 01:01 PM To cc Subject RE: [Histonet] MUM1 Elaine Haven't heard of this protein before. Does it operate against birth mothers, mother in laws or mothers in general? Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Elaine Dooley Sent: Thursday, August 03, 2006 12:57 PM To: Histonet@pathology.swmed.edu Subject: [Histonet] MUM1 Dear Histonetters, I am getting ready to purchase an antibody to MUM1 Protein. I noticed that they suggest 1mMEDTA at 9.0ph for antigen retrieval. Has anyone tried Ventana CC1 for antigen retrieval? Any suggestions of dilutions and pretreatments would be appreciated. Elaine Dooley HTL Shands Teaching Hosp. 352-265-0111 ext 72117 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jdr43 <@t> omega.med.yale.edu Thu Aug 3 13:59:55 2006 From: jdr43 <@t> omega.med.yale.edu (jdr43@omega.med.yale.edu) Date: Thu Aug 3 13:59:58 2006 Subject: [Histonet] pfa vs. nbf Message-ID: <1154631595.44d247ab4ff4f@webmail.med.yale.edu> Hi all- I'm a medical student doing immunohistochemistry studies on vascular grafts in mice. We are currently pressure fixing these mouse vessels in 4% paraformaldehyde, fixing them in 10% neutral buffered formalin overnight, and then paraffin embedding these samples (we cannot do frozen b/c the grafts tend to shred when we cut them). I was wondering if it is better to transfer them to 10% NBF for overnight fixation or if we can just keep them in 4% PFA overnight. Does it make a difference? Also, the plan is to use immunofluorescence. Is PFA or NBF better for this, or again is there no difference? As always, any advice or help would be much appreciated. Thanks in advance. Jason New Haven, CT 06510 From plaurie <@t> benaroyaresearch.org Thu Aug 3 16:21:29 2006 From: plaurie <@t> benaroyaresearch.org (Patrick Laurie) Date: Thu Aug 3 16:21:47 2006 Subject: [Histonet] Cytoarchitectural analysis of axons and thick sectioning of neural tissue Message-ID: Dear histonet professionals, My PI and I have been accumulating a large bank of 40 human brains and spinal cords with ALS and controls with non-neurologic conditions. Certain areas of the brain and the entire cord has either been fixed in 10% NBF and then processed through to paraffin or frozen for future molecular studies. My PI has been reading a number of papers, and noticed that usually the brains are sectioned very thick (20+ microns). I believe that I understand why, but I have tried to section our samples that thick, and I have had a lot of problems. Would it be easier with a softer paraffin, (I am using Tissue-Tek's VIP brand), a different microtome (maybe a sliding, or a vibratome) or some other method I haven't considered. Secondly, he wants to embed some of our spinal cord specimens in Epon for cytoarchitectural analysis of the spinal tracts. Typical procedures I have seen require the tissue to be fixed in gluteraldehyde, mine are fixed in formalin. Are the two methods interchangeable, or could I postfix in gluteraldehyde and then embed in epon? Thanks in advance for any tips. Patrick Laurie, HT (ASCP) Neurogenomics Laboratory Benaroya Research Institute 1201 9th Ave Seattle, Wa 98101 (206) 341-0681 From tncperfect2 <@t> comcast.net Thu Aug 3 17:23:14 2006 From: tncperfect2 <@t> comcast.net (tncperfect2@comcast.net) Date: Thu Aug 3 17:23:45 2006 Subject: [Histonet] POC Specialty Lab Message-ID: <080320062223.27373.44D27752000A0C5600006AED2200760180CD9B0C0A009D0A9F0C029B@comcast.net> Hi Histonetters, Is there a lab somewhere that processes strictly POC tissue? Thanks, Carolyn From SHARON.OSBORN <@t> SPCORP.COM Thu Aug 3 18:01:27 2006 From: SHARON.OSBORN <@t> SPCORP.COM (Osborn, Sharon) Date: Thu Aug 3 18:01:32 2006 Subject: [Histonet] Orgasm..oops Organism controls Message-ID: <9A919A5D70313A4D9C56A025710874080C736C@kenmsg40.us.schp.com> Janci, Look in Lee Luna's Histopathologic Methods and Color Atlas of Special Stains and Tissue Artifacts beginning on page 566 then page 572. Lee says on page 566 that beef jerky can be used as a Gram +/- control. On page 566 he give the recipe for making it in the lab. He also provides instructions for making controls for other special stains. I find this is an excellent reference book to have. :-) sharon osborn DNAX, SP BioPharma Palo Alto, CA 650.496.6539 Message: 1 Date: Thu, 03 Aug 2006 12:47:56 -0400 From: "Janci Wellborn" Subject: [Histonet] Just wondering if anyone out there has a procedure on making your own control tissue for organisms? To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=us-ascii Just wondering if anyone out there has a procedure on making your own control tissue for organisms? Any help in this area would be appreciated. Thank-you, Janci Wellborn, BS, BSeD, HTL Dunes Medical Lab Dakota Dunes, SD ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From zhengl02 <@t> student.ucr.edu Fri Aug 4 03:42:46 2006 From: zhengl02 <@t> student.ucr.edu (Lei Zheng) Date: Fri Aug 4 03:42:56 2006 Subject: [Histonet] Visualizing dual endogenous Fluorescent Proteins in mice tissue (fresh or fixed) Message-ID: <200608040142461562372@student.ucr.edu> Hey, I am new. Got a newbie question. I have shed much blood searching for info on how to visualiz these: transgenic mice tissue with endogenous mRFP1 (monomeric RFP by R.Y Tsien) in cytoplasm AND eGPF on cell membranes. Currently, I fix the fresh tissue(thin sections) in 4%PFA followed by cryosections. The mRFP1 look good, but eGFP channel is filled with autofluroescence (by PFA I guess). Using NaBH4 did not help with eGFP. I am really dying for a protocl that could: 1) Maximize the eGFP intensity against background 2) Minimize the green channel autofluorescence 3) Maintain the membrane localization of eGFP so that cell morphology can be kept 4) No immunostaining can be used (rule of my game...), must be FP-derive fluroescence I read much and notice several taboos: 1) no organic solvent in the processing (kills FPs) 2) NaBH4 may help (actually not for me) 3) Zinc buffer (no formalin) may help maintain membrane bound FP? antigen? 4) Fresh tissue snap frozen then sectioned yields good eGFP? I am very lost and bombarded with information from lots of people. If you are a "mice people" working with endogenous eGFP, can you throw me a straw please? The key things here are the mRFP has no problem, but the eGFP is membran-bound and maybe of low level.....and I need to be able to see that eGFP!!! Much thanks Zak/UCR From Olivier.Leroux <@t> UGent.be Fri Aug 4 04:45:52 2006 From: Olivier.Leroux <@t> UGent.be (Olivier Leroux) Date: Fri Aug 4 04:46:03 2006 Subject: [Histonet] Tannines Message-ID: <1154684752.44d31750b34a6@webmail.ugent.be> Hello, Is there anybody who knows how to visualize tannines in 7?m Technovit sections of plant material? regards, Olivier -- Olivier Leroux Ghent University Department of Biology - Pteridological Section K.L. Ledeganckstraat 35 B-9000 Belgium http://www.pteridology.ugent.be Olivier.Leroux@UGent.be From failm <@t> musc.edu Fri Aug 4 07:59:33 2006 From: failm <@t> musc.edu (Mildred Fail) Date: Fri Aug 4 08:02:35 2006 Subject: [Histonet] Whole embyo mouse processing Message-ID: One of our research techs has receieved 18 mouse embryos in NBF. They need to be processed whole . Fixation time, decal, processing time advice would be greatly appreciated. She plans to fix the specimens over the weekend. Pleaes respond directly to her romanom@musc.edu Thanks in advance for your help Rena Fail From RSRICHMOND <@t> aol.com Fri Aug 4 12:06:54 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Fri Aug 4 12:07:15 2006 Subject: [Histonet] POC specialty lab Message-ID: <429.75cb0b7.3204d8ae@aol.com> Carolyn asks: >>s there a lab somewhere that processes strictly POC tissue?<< Yes, there is. If you'll reply to me privately I'll tell you how to contact them. Bob Richmond From Melissa.Gonzalez <@t> cellgenesys.com Fri Aug 4 13:03:24 2006 From: Melissa.Gonzalez <@t> cellgenesys.com (Melissa Gonzalez) Date: Fri Aug 4 13:03:32 2006 Subject: [Histonet] RE: pfa vs. formalin Message-ID: Hi Jason, I was taught by histotechs that 10%Neutral Buffered Formalin is the gold standard vs paraformaldehyde, because it is optimally buffered to exchange with tissue fluids during the fixation process, and that unbuffered fixatives can result in artifacts which you may find microscopically in the tissue slices after stainings. How major/minor this detail turns out overall in the grand scheme of things, I don't really know. I've never seen the direct compare and contrast, for example in H&E sections comparing both fixatives. I have found a supplier of 10% Buffered Paraformaldehyde, from Newcomer Supply, which I use routinely for immunofluorescence of perfused, and cryoprotected samples. So then I would like to know, is there a technical difference between 10% NBF (formalin) vs 10% NBP (paraformaldehyde)? thanks Melissa From bhewlett <@t> cogeco.ca Fri Aug 4 13:26:06 2006 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Fri Aug 4 13:26:11 2006 Subject: [Histonet] RE: pfa vs. formalin References: Message-ID: <003601c6b7f3$73de6b10$6500a8c0@mainbox> Melissa, There is NO such thing as a solution of paraformaldehyde. As has been stated many times, paraformaldehyde is a solid polymerized form of formaldehyde. When paraformaldehyde is dissolved in water, it de-polymerizes to form formaldehyde. The formaldehyde in turn hydrates to become methylene glycol. 10% NBF is, for all practical purposes, the same whether made from commercial formalin (37-40% formaldehdye) or from solid paraformaldehyde. Bryan ----- Original Message ----- From: "Melissa Gonzalez" To: Cc: Sent: Friday, August 04, 2006 2:03 PM Subject: [Histonet] RE: pfa vs. formalin Hi Jason, I was taught by histotechs that 10%Neutral Buffered Formalin is the gold standard vs paraformaldehyde, because it is optimally buffered to exchange with tissue fluids during the fixation process, and that unbuffered fixatives can result in artifacts which you may find microscopically in the tissue slices after stainings. How major/minor this detail turns out overall in the grand scheme of things, I don't really know. I've never seen the direct compare and contrast, for example in H&E sections comparing both fixatives. I have found a supplier of 10% Buffered Paraformaldehyde, from Newcomer Supply, which I use routinely for immunofluorescence of perfused, and cryoprotected samples. So then I would like to know, is there a technical difference between 10% NBF (formalin) vs 10% NBP (paraformaldehyde)? thanks Melissa _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pmarcum <@t> vet.upenn.edu Fri Aug 4 13:29:33 2006 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Fri Aug 4 13:29:42 2006 Subject: [Histonet] RE: pfa vs. formalin In-Reply-To: References: Message-ID: <6.1.1.1.2.20060804141210.019bead0@mail.vet.upenn.edu> At 02:03 PM 8/4/2006, Melissa Gonzalez wrote: >Hi Jason, >I was taught by histotechs that 10%Neutral Buffered Formalin is the gold >standard vs paraformaldehyde, because it is optimally buffered to exchange >with tissue fluids during the fixation process, and that unbuffered >fixatives can result in artifacts which you may find microscopically in >the tissue slices after stainings. How major/minor this detail turns out >overall in the grand scheme of things, I don't really know. I've never >seen the direct compare and contrast, for example in H&E sections >comparing both fixatives. >I have found a supplier of 10% Buffered Paraformaldehyde, from Newcomer >Supply, which I use routinely for immunofluorescence of perfused, and >cryoprotected samples. >So then I would like to know, is there a technical difference between 10% >NBF (formalin) vs 10% NBP (paraformaldehyde)? > >thanks >Melissa >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Melissa, All formaldehyde starts as a powder or flaked paraformaldehyde that is convert by heating it in a liquid. This can be in water or as a concentrated solution to be added to a buffer. Most formulas for PFA are 2% to 4% or 2 to 4 grams on paraformaldehyde powder dissolved at 60C and adjusted with a buffer to the pH as required for the protocol. It has no methanol added to stabilize the formaldehyde solution and is preferred by EM and most flow cytometry people. Some will argue the stabilized solution is fine and this really a choice by the laboratory using the solution. The 10% NBF is made from 37 to 40% formaldehyde solution prepared commercially that has been stabilized with methanol (in most cases 10 to 15%). It is not a true 10% solution as it is 10mL of the 37 to 40% formaldehyde in 90mL water or buffer. The term 10% NBF is based on the volumes used to make the solution and is in reality a 3.7 to 4.0% formaldehyde in buffer or water as it is made. It is an excellent fixative and is preferred by histologists and pathologist as the majority of papers have been written and are based on 10% NBF. This is an overview I use to explain to students the differences in PFA and NBF. Hope it helps. Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From weneng2004 <@t> yahoo.com Fri Aug 4 13:29:56 2006 From: weneng2004 <@t> yahoo.com (wen eng) Date: Fri Aug 4 13:30:00 2006 Subject: [Histonet] Thank you! Message-ID: <20060804182956.88011.qmail@web53408.mail.yahoo.com> Many thanks to all who recommend TUNEL kits to me! They are really helpful and saved me much time. I really appreciate it! Wen --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail Beta. From PMonfils <@t> Lifespan.org Fri Aug 4 13:36:00 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Aug 4 13:36:06 2006 Subject: [Histonet] RE: pfa vs. formalin Message-ID: <09C945920A6B654199F7A58A1D7D1FDE01717777@lsexch.lsmaster.lifespan.org> The only difference is that commercial formaldehyde solution contains a stabilizer, usually 10 to 15% methanol. Therefore, 10% NBF made up from such a solution contains 1 to 1.5% methanol, while 10% buffered NBP contains nothing but water, formaldehyde, and buffer salts. For routine morphological studies this makes no difference whatsoever. But for some special studies the presence of a small amount of methanol could be deleterious, and for any such procedures paraformaldehyde would be preferable. From sjchtascp <@t> yahoo.com Fri Aug 4 14:00:07 2006 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Fri Aug 4 14:00:12 2006 Subject: [Histonet] NFPA Rating Message-ID: <20060804190007.74231.qmail@web38209.mail.mud.yahoo.com> I have noticed some MSDS's do not have NFPA ratings, especially for dyes. I'm putting together for the Company I work for an MSDS Manual. Can anyone suggest a good reference site. Many thanks, --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail Beta. From bhewlett <@t> cogeco.ca Fri Aug 4 14:04:37 2006 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Fri Aug 4 14:04:43 2006 Subject: [Histonet] RE: pfa vs. formalin References: <09C945920A6B654199F7A58A1D7D1FDE01717777@lsexch.lsmaster.lifespan.org> Message-ID: <004801c6b7f8$d533c4f0$6500a8c0@mainbox> Hi Paul, Actually, commercial formaldehyde solutions may contain anywhere from 5-15% methanol. It often depends on geographic location and what time of year the reagent is shipped. Over the years, I have performed many side-by-side studies of 4% phosphate buffered formaldehydes, prepared from both commercial formaldehyde solutions and from paraformaldehyde. The fixatives were used for a wide range of investigations including morphology using routine oversight and many special stains, histochemistry, IHC, ISH and EM. In NO case could I or anyone else detect any difference Are you aware of any definitive studies which prove that the presence of 0.5-1.5% methanol is deleterious for any investigation? Regards, Bryan ----- Original Message ----- From: "Monfils, Paul" To: Sent: Friday, August 04, 2006 2:36 PM Subject: RE: [Histonet] RE: pfa vs. formalin > The only difference is that commercial formaldehyde solution contains a > stabilizer, usually 10 to 15% methanol. Therefore, 10% NBF made up from > such a solution contains 1 to 1.5% methanol, while 10% buffered NBP > contains > nothing but water, formaldehyde, and buffer salts. For routine > morphological studies this makes no difference whatsoever. But for some > special studies the presence of a small amount of methanol could be > deleterious, and for any such procedures paraformaldehyde would be > preferable. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From sdann <@t> UCSD.Edu Fri Aug 4 14:41:00 2006 From: sdann <@t> UCSD.Edu (sdann@UCSD.Edu) Date: Fri Aug 4 14:41:09 2006 Subject: [Histonet] Autotechnicon Mono manual Message-ID: <200608041941.k74Jf0tC011051@smtp.ucsd.edu> Does anyone have an Autotechnicon Mono Tissue Processor (Model 2A) operation manual or know the purpose of the 1HR/24HR switch. We recently acquired a well maintained processor and would like a copy of the manual. Please contact me at sdann@ucsd.edu if you have one to sell or give. Thanks, Sara From mtitford <@t> aol.com Fri Aug 4 17:04:43 2006 From: mtitford <@t> aol.com (mtitford@aol.com) Date: Fri Aug 4 17:04:52 2006 Subject: [Histonet] Autotechnicon program Message-ID: <8C886291FF8C588-11BC-F00@MBLK-R08.sysops.aol.com> Sara at UCSD (University of California at San Diego maybe?) asks about Technicon processors. The autotechnicons were programmed by using a round disc with notches on the edge. During processing, as the disc rotated a peg falls into the notch and activates the machine to advance the basket of cassettes to the next solution. Both 24 hour and 1 hour programmes were available. I seem to remember there was a separate disc for the one hour program which had a broad ridge of metal on the BACK of the disc. We still have a technicon we use only for CJD cases but I don't know what model it is or if we have a manual for it (And its Friday afternoon and I am too lazy to walk up the hall to look...!) Mike Titford USA Pathology Mobile AL USA ________________________________________________________________________ Check out AOL.com today. Breaking news, video search, pictures, email and IM. All on demand. Always Free. From jmaass <@t> frii.com Fri Aug 4 22:39:14 2006 From: jmaass <@t> frii.com (Janet Maass) Date: Fri Aug 4 22:39:19 2006 Subject: [Histonet] Autotechnicon Mono manual References: <200608041941.k74Jf0tC011051@smtp.ucsd.edu> Message-ID: <001a01c6b840$b91a8ae0$0200a8c0@oemcomputer> Sara I believe the same motors and clocks where put in the mono and dual level Autotechnicon. There was a slide holder that fit on the Technicon. It had two large springs in which you put your slides in and then release the spring to tighten and it would hold your slides in place. This slide holder fit into the hanging device that the basket fits into and the slides would dip into the containers. I would use the 24 hour clock at night and the 1 hour clock during the day for staining. I had two xylene jars for the beginning xylene then I would start the slides in absolute alcohol as there were not enough containers on the Technicon.. Janet Maass From MVaughan4 <@t> ucok.edu Sat Aug 5 16:57:31 2006 From: MVaughan4 <@t> ucok.edu (MVaughan4@ucok.edu) Date: Sat Aug 5 16:57:59 2006 Subject: [Histonet] pfa vs. formalin In-Reply-To: Message-ID: Bryan, Molecular Probes sells various phallotoxin probes that lose effectiveness binding to f-actin microfilaments when methanol is used as a fixative or is part of a fixative (10% NBF). For these purposes I dilute 16% formaldehyde, MeOH free, sold by Polysciences in sealed ampules, diluted 1:1 with water, then that 8% mixture further diluted 1:1 with 0.2M Phosphate buffer pH 7.4. It works great as a fixative. Mel Melville B. Vaughan, Ph. D. Assistant Professor Department of Biology University of Central Oklahoma 100 N. University Drive Edmond, OK 73034 http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm From Linresearch <@t> aol.com Sat Aug 5 17:58:37 2006 From: Linresearch <@t> aol.com (Linresearch@aol.com) Date: Sat Aug 5 17:58:49 2006 Subject: [Histonet] Renal Necrosis Message-ID: <417.80a33d7.32067c9d@aol.com> Hello, Would anyone familiar with antibodies used for renal necrosis be willing to share source of Abs: RPA, GST, Clusterin and protocol? I am working with FFPE rat tissues. Lin From Eric <@t> ategra.com Fri Aug 4 13:03:51 2006 From: Eric <@t> ategra.com (Eric Dye (ext 223)) Date: Sun Aug 6 00:33:48 2006 Subject: [Histonet] Histology Jobs in your area Message-ID: Hi - Fellow-Histonetters How are you? Below is the updated list of Histology j o b s, All openings are Dayshift Monday thru Friday unless indicated otherwise. All of these jobs are looking to move quickly so if your interested call me A S A P... If you are interested in any of the Histology jobs listed below or anywhere else - please call me at 800-466-9919 ext 223 or cell - 407-756-5507. Most HistoTech j o b s are permanent full-time unless indicated otherwise Here are some of my Newest Histology Jobs: ------------------------------------------------------ Ohio (Southern) - perm - Bench Histotech Washington, D.C - Histotech -perm and temp( 3-6 months) New York (Long Island) - HistoTech - perm Northern New Jersey - HistoTech and Cyto Tech - perm Boston Mass - HistoTech - one Senior HistoTech, One not so Senior Histotech Massachusetts (North of Boston) - perm - Bench Histotech Minnesota (Twin Cities area - perm - Bench Histotech - Biotech/research - Make your own schedule!!! Southeast Florida - perm - HistoTech Southwest Florida - perm - Bench Histotech/ Supervisor Florida, West Coast - both temp & perm openings- Bench Histotech New Hampshire perm openings - Bench Histotech (opportunity to learn Mohs!) ---- end list of HistoTech Opportunities --- If you are interested in any of the Histology jobs listed above - please call me at 800-466-9919 ext 223 or cell - 407-756-5507. Thank you, Eric Dye-Sr Allied Healthcare Recruiter 800-466-9919 ext 223 or Cell - 407-756-5507 P.S.: Feel free to pass along this email and my phone number to anyone who you think might be interested. P.S.S.: The clients are currently interviewing - and the job will close soon - so if you are interested, please call me today at 1-800-466-9919 ext 229 --------------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing 800 466 9919 ext 223 - voice 407 671 6075 - fax [1]eric@ategra.com To Learn More About Ategra: [2]http://www.ategra.com ---------------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. ---------------------------------------------------------------- ---------------------------------------------------------------- Note: this message is intended for: Fellow-Histonetters at histonet@lists.utsouthwestern.edu ---------------------------------------------------------------- References 1. mailto:eric@ategra.com 2. http://www.ategra.com/ From nickandmanda <@t> paradise.net.nz Sun Aug 6 03:42:17 2006 From: nickandmanda <@t> paradise.net.nz (nick bowden) Date: Sun Aug 6 03:42:27 2006 Subject: [Histonet] HSV Controls In-Reply-To: <20060801170516.81E7D9A7FBC@smtp-1.paradise.net.nz> Message-ID: <20060806084218.A40D563CF05@smtp-2.paradise.net.nz> Hello! Wow.. this is my very first time on histonet and I have a few technical things I would like to ask for help with but first is: Could anyone please tell me where I could purchase HSV I and II control sections for immunohistochemical staining?? Our previous supplier no longer supplies!! Thanx, much appreciated! Amanda From WillCavett <@t> mhd.com Sun Aug 6 09:50:11 2006 From: WillCavett <@t> mhd.com (Cavett, Will) Date: Sun Aug 6 09:49:20 2006 Subject: [Histonet] Unsubscribe... In-Reply-To: <417.80a33d7.32067c9d@aol.com> Message-ID: <293C7C19EFF7D611AE1A0002A53F81141BF1B929@omega.mhd.com> Please unsubscribe me from this list. *********************************************************************** This electronic transmission contains information from Methodist Health System and should be considered confidential and privileged. The information contained in the above messages is intended only for the use of the individual(s) and entity(ies) named above. If you are not the intended recipient, be aware that any disclosure, copying, distribution, or use of this information is prohibited. If you receive this transmission in error, please notify the sender immediately by return e-mail. Methodist Health System, its subsidiaries and affiliates hereby claim all applicable privileges related to the transmission of this communication. From Shirley_PHUA <@t> hsa.gov.sg Sun Aug 6 13:06:11 2006 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Sun Aug 6 13:06:28 2006 Subject: [Histonet] Shirley is away : 7-11 August 2006 (Monday-Friday) Message-ID: I will be out of the office from 08/07/2006 to 08/11/2006. I will return on 14 August 2006 (Monday). Pathologists : I will process your requests when I return. Otherwise, if urgent, please forward your mail to henry_kyaw@hsa.gov.sg Regrets for the inconveniences caused. From zacharyweil <@t> yahoo.com Sun Aug 6 16:00:00 2006 From: zacharyweil <@t> yahoo.com (Zach Weil) Date: Sun Aug 6 16:00:08 2006 Subject: [Histonet] Hamster Microglia Message-ID: <20060806210001.25301.qmail@web50608.mail.yahoo.com> Has anyone been able to succesfully stain hamster brain tissue for activated microglia? I have tried the Serotec anti-OX42 antibody with no success. Thanks, Zach --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail Beta. From LuckG <@t> empirehealth.org Sun Aug 6 16:55:19 2006 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Sun Aug 6 16:55:25 2006 Subject: [Histonet] FW: Job Opening Message-ID: <6BB8BC4519AAB844B174FC739A679BBC508EC4@IRMEXCH01.irm.inhs.org> Hello all, We have a full time, days, M-F histotech position open here at Deaconess Medical Center in Spokane, Washington. If you'd like more specifics about the job duties and the lab environment in general please contact me personally. I have included 3 web links below you can just click on to go to websites where you can learn more about Empire Health Services, Deaconess (where you can fill out an application on-line) and Spokane and the surrounding area. This a great place to work and plant your roots. Thanks!! p.s. To get more specific details about the job description, duties and work environment look at the job posting on the www.nsh.org website for Empire Health Services/Deaconess Medical Center in Spokane, WA www.visitspokane.c om www.deaconessmc.org www.empirehealth.org Greg Luck Anatomic Path Spvr Deaconess Med Cntr 800 W. 5th Ave Spokane, WA 99204 Offc 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmc.org From DCHH728 <@t> aol.com Sun Aug 6 17:46:13 2006 From: DCHH728 <@t> aol.com (DCHH728@aol.com) Date: Sun Aug 6 17:46:24 2006 Subject: [Histonet] unscribe Message-ID: From vaskomark <@t> yahoo.com Sun Aug 6 21:37:53 2006 From: vaskomark <@t> yahoo.com (mark vasko) Date: Sun Aug 6 21:37:58 2006 Subject: [Histonet] Eliminate "natural" fluorescence in heart cells Message-ID: <20060807023753.41980.qmail@web32412.mail.mud.yahoo.com> Good day, I am trying to eliminate "natural" autofluorescence from rat heart cells under an olympus fluorescence microscope. I have a particular set of labeled cells I am trying to view but autofluorescence from the rat heart is quite strong. I have histology slide preparations as well as single cell suspensions which I view under the olmpus with a disposable hemocytometer. Thank you for any suggestions. Sincerely, Mark M. Vasko --------------------------------- How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. From vaskomark <@t> yahoo.com Sun Aug 6 21:42:03 2006 From: vaskomark <@t> yahoo.com (mark vasko) Date: Sun Aug 6 21:42:06 2006 Subject: Fwd: [Histonet] Eliminate "natural" fluorescence in heart cells Message-ID: <20060807024203.27771.qmail@web32407.mail.mud.yahoo.com> Good day, I am trying to eliminate "natural" autofluorescence from rat heart cells under an olympus fluorescence microscope. I have a particular set of labeled cells I am trying to view but autofluorescence from the rat heart is quite strong. I have histology slide preparations as well as single cell suspensions which I view under the olmpus with a disposable hemocytometer. Thank you for any suggestions. Sincerely, Mark M. Vasko --------------------------------- Yahoo! Music Unlimited - Access over 1 million songs.Try it free. From cforster <@t> umn.edu Sun Aug 6 21:47:51 2006 From: cforster <@t> umn.edu (Colleen Forster) Date: Sun Aug 6 21:47:55 2006 Subject: [Histonet] Vysis Her2 FISH kits Message-ID: <44D6A9D7.2080205@umn.edu> Hello Histonetters, I have a question for you, I am doing FISH Her2 with the Vysis kit. Can anyone tell me what the concentration and pH of the "Ptretreatment" (Sodium Thiocyante) solution is? Are any of you making up your own?? Thanks in advance, Colleen Forster From melissa.mazan <@t> tufts.edu Mon Aug 7 07:13:17 2006 From: melissa.mazan <@t> tufts.edu (Melissa Mazan) Date: Mon Aug 7 07:13:23 2006 Subject: [Histonet] PFA v. formalin In-Reply-To: <200608061707.k76H79jZ018583@mail-proofpoint-2a.usg.tufts.edu> References: <200608061707.k76H79jZ018583@mail-proofpoint-2a.usg.tufts.edu> Message-ID: <44D72E5D.2080906@tufts.edu> How long can the tissues be left in the PFA (I realize it is not really PFA once in solution, so I suppose it should be called fresh formalin)? We're doing immunofluorescence using standard buffered formalin, some on archived tissues that have been sitting in formalin for several weeks. However, I keep hearing that freshly made formalin from PFA is preferable, but that it needs to be processed into wax blocks fairly rapidly? Thanks for any advice - Melissa histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. pfa vs. formalin (MVaughan4@ucok.edu) > 2. Renal Necrosis (Linresearch@aol.com) > 3. Histology Jobs in your area (Eric Dye (ext 223)) > 4. HSV Controls (nick bowden) > 5. Unsubscribe... (Cavett, Will) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Sat, 5 Aug 2006 16:57:31 -0500 > From: MVaughan4@ucok.edu > Subject: [Histonet] pfa vs. formalin > To: histonet@lists.utsouthwestern.edu > Message-ID: > > Content-Type: text/plain; charset="US-ASCII" > > Bryan, > Molecular Probes sells various phallotoxin probes that lose effectiveness > binding to f-actin microfilaments when methanol is used as a fixative or > is part of a fixative (10% NBF). For these purposes I dilute 16% > formaldehyde, MeOH free, sold by Polysciences in sealed ampules, diluted > 1:1 with water, then that 8% mixture further diluted 1:1 with 0.2M > Phosphate buffer pH 7.4. It works great as a fixative. > Mel > > Melville B. Vaughan, Ph. D. > Assistant Professor > Department of Biology > University of Central Oklahoma > 100 N. University Drive > Edmond, OK 73034 > http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm > > ------------------------------ > > Message: 2 > Date: Sat, 5 Aug 2006 18:58:37 EDT > From: Linresearch@aol.com > Subject: [Histonet] Renal Necrosis > To: histonet@pathology.swmed.edu > Message-ID: <417.80a33d7.32067c9d@aol.com> > Content-Type: text/plain; charset="US-ASCII" > > Hello, > Would anyone familiar with antibodies used for renal necrosis be willing to > share > source of Abs: RPA, GST, Clusterin and protocol? > I am working with FFPE rat tissues. > Lin > > > ------------------------------ > > Message: 3 > Date: Fri, 4 Aug 2006 14:03:51 -0400 > From: Eric Dye (ext 223) > Subject: [Histonet] Histology Jobs in your area > To: Histonetters > Message-ID: > Content-Type: text/plain > > > Hi - Fellow-Histonetters > > How are you? > Below is the updated list of Histology j o b s, All openings are > Dayshift Monday thru Friday unless indicated otherwise. All of these > jobs are looking to move quickly so if your interested call me A S A > P... > If you are interested in any of the Histology jobs listed below or > anywhere else - please call me at 800-466-9919 ext 223 or cell - > 407-756-5507. > Most HistoTech j o b s are permanent full-time unless indicated > otherwise > Here are some of my Newest Histology Jobs: > ------------------------------------------------------ > Ohio (Southern) - perm - Bench Histotech > Washington, D.C - Histotech -perm and temp( 3-6 months) > New York (Long Island) - HistoTech - perm > Northern New Jersey - HistoTech and Cyto Tech - perm > Boston Mass - HistoTech - one Senior HistoTech, One not so Senior > Histotech > Massachusetts (North of Boston) - perm - Bench Histotech > Minnesota (Twin Cities area - perm - Bench Histotech - > Biotech/research - Make your own schedule!!! > Southeast Florida - perm - HistoTech > Southwest Florida - perm - Bench Histotech/ Supervisor > Florida, West Coast - both temp & perm openings- Bench Histotech > New Hampshire perm openings - Bench Histotech (opportunity to learn > Mohs!) > > ---- end list of HistoTech Opportunities --- > If you are interested in any of the Histology jobs listed above - > please call me at 800-466-9919 ext 223 or cell - 407-756-5507. > Thank you, > Eric Dye-Sr Allied Healthcare Recruiter > 800-466-9919 ext 223 or Cell - 407-756-5507 > P.S.: Feel free to pass along this email and my phone number to anyone > who you think might be interested. > P.S.S.: The clients are currently interviewing - and the job will > close soon - so if you are interested, please call me today at > 1-800-466-9919 ext 229 > --------------------------------------------------------------- > Ategra Systems Inc > Specialists in Permanent & Contract Staffing > 800 466 9919 ext 223 - voice > 407 671 6075 - fax > [1]eric@ategra.com > To Learn More About Ategra: > [2]http://www.ategra.com > ---------------------------------------------------------------- > If you received this by mistake, or if you wish > not to hear from me, please shoot me a mail > to let me know and I'll not mail you again. > ---------------------------------------------------------------- > ---------------------------------------------------------------- > Note: this message is intended for: > Fellow-Histonetters at histonet@lists.utsouthwestern.edu > ---------------------------------------------------------------- > > References > > 1. mailto:eric@ategra.com > 2. http://www.ategra.com/ > > > ------------------------------ > > Message: 4 > Date: Sun, 06 Aug 2006 20:42:17 +1200 > From: nick bowden > Subject: [Histonet] HSV Controls > To: histonet@lists.utsouthwestern.edu > Message-ID: <20060806084218.A40D563CF05@smtp-2.paradise.net.nz> > Content-Type: text/plain; charset=us-ascii > > Hello! > > Wow.. this is my very first time on histonet and I have a few technical > things I would like to ask for help with but first is: Could anyone please > tell me where I could purchase HSV I and II control sections for > immunohistochemical staining?? Our previous supplier no longer supplies!! > Thanx, much appreciated! > Amanda > > > > > > ------------------------------ > > Message: 5 > Date: Sun, 6 Aug 2006 09:50:11 -0500 > From: "Cavett, Will" > Subject: [Histonet] Unsubscribe... > To: , > Message-ID: <293C7C19EFF7D611AE1A0002A53F81141BF1B929@omega.mhd.com> > Content-Type: text/plain; charset="us-ascii" > > > Please unsubscribe me from this list. > > *********************************************************************** > > This electronic transmission contains information from Methodist Health > System and should be considered confidential and privileged. The > information contained in the above messages is intended only for the > use of the individual(s) and entity(ies) named above. If you are not > the intended recipient, be aware that any disclosure, copying, > distribution, or use of this information is prohibited. If you receive > this transmission in error, please notify the sender immediately by > return e-mail. Methodist Health System, its subsidiaries and > affiliates hereby claim all applicable privileges related to the > transmission of this communication. > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 33, Issue 9 > *************************************** -- Melissa R. Mazan, DVM, Diplomate ACVIM Associate Professor of Large Animal Medicine Director of Sports Medicine Cummings School of Veterinary Medicine Tufts University 200 Westborough Road North Grafton, MA 01536 Tel: 508-839-5395 Fax: 508-839-7922 Email: melissa.mazan@tufts.edu From Julie.Sanders <@t> va.gov Mon Aug 7 08:31:30 2006 From: Julie.Sanders <@t> va.gov (Sanders, Julie, VHACIN) Date: Mon Aug 7 08:31:36 2006 Subject: [Histonet] Antibody VEGF In-Reply-To: <526j9a$16g2gp@mtasac1.sac.net.va.gov> Message-ID: <2D4ACE41DEFE93428F23D77988EFBCB50E6058@VHAV10MSGA2.v10.med.va.gov> Is anyone using the antibody Vascular Endothelial Growth Factor (VEGF)? We want to do some research with this on the Ventana Benchmark and I would be interested in any protocols, what clone your using and vendors that have this antibody. Thanks in advance, Julie Julie A. Sanders, BA, HT(ASCP) Supervisor Anatomic Pathology VAMC Cincinnati 3200 Vine St. Cincinnati, Ohio 45220 From pmarcum <@t> vet.upenn.edu Mon Aug 7 10:18:02 2006 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Mon Aug 7 10:18:10 2006 Subject: [Histonet] Pennsylvaina Fall Symposium October, 2006 Message-ID: <6.1.1.1.2.20060807105758.019c65d0@mail.vet.upenn.edu> We in the Pennsylvaina Histotechnology Society are proud to announce the opening of our web site at www.pahisto.org and the program for our October. 2006 meeting in Pittsburgh. We will be adding to the site and updating all areas of the program to include our vendors very soon. The program is currently not in pdf. and we apologize. PHS felt it was more important to get the program out for review than to hold for several more weeks while we are setting up. The current program is in Microsoft Word and can be printed off for those who need now. We hope this helps you decide to join us for one or all of the three days. PHS is using the Thursday for those who need more information on CPT Coding, CAP Inspections and Ergonomics for laboratories and supervisors. The class size is not limited to PHS, constituent state societies or NSH members. These seminars are being run over the course of one day with not competition from other seminars to allow those wishing to come and not miss more technical or specialized seminars. If you have a laboratory manager who is not in the histology department and still needs to understand CPT codes or CAP inspections for Histology this will be a good place to learn and talk to others struggling (not that any of us do) with these areas of laboratory management. The seminars on Friday and Saturday will be more technical and some just plain fun. We will have a lecture on the Bog People of Nothern Europe (mummies) on Friday. Special hands on with Mohs grossing and sectioning and then just frozen sectioning for the routine clinical area later. Want to know about grossing for the histologist we have a seminar for that too. We attempted to talk the Dr. Gore into leaving his title on DNA, PCR as ISHY, FISHY, PCR and he declined. It is on Friday afternoon. The IHC talks are for those starting who still have questions (Friday morning) and those more advanced with double and triple staining (Saturday morning). Saturday will also have a room devoted to CSI type lectures. Check the web site www.pahisto.org and join us for one or all three days. Thanks to LabVision for setting the basis of the web site and letting us fill in the blanks. Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From anh2006 <@t> med.cornell.edu Mon Aug 7 10:36:17 2006 From: anh2006 <@t> med.cornell.edu (Andrea T. Hooper) Date: Mon Aug 7 10:36:29 2006 Subject: [Histonet] RE: pfa vs. formalin In-Reply-To: <09C945920A6B654199F7A58A1D7D1FDE01717777@lsexch.lsmaster.lifespan.org> References: <09C945920A6B654199F7A58A1D7D1FDE01717777@lsexch.lsmaster.lifespan.org> Message-ID: I have found that for many mouse antigens, the presence of this methanol in 10% NBF is deleterious when doing fixed frozen immunohistochemistry (a necessary evil for when we work with muscle and bone). So when doing fixed frozen IHC I always make up 4% PFA in PBS from a 16% PFA ampoule. Works very well. When fixing specimens for paraffin IHC we always use 10% NBF with no problems. >The only difference is that commercial formaldehyde solution contains a >stabilizer, usually 10 to 15% methanol. Therefore, 10% NBF made up from >such a solution contains 1 to 1.5% methanol, while 10% buffered NBP contains >nothing but water, formaldehyde, and buffer salts. For routine >morphological studies this makes no difference whatsoever. But for some >special studies the presence of a small amount of methanol could be >deleterious, and for any such procedures paraformaldehyde would be >preferable. > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- From langston <@t> hawaii.edu Mon Aug 7 13:03:41 2006 From: langston <@t> hawaii.edu (Ross Christian Langston) Date: Mon Aug 7 13:03:47 2006 Subject: [Histonet] Digital Camera Setup for Student Microscopes Message-ID: Hello- I am looking for an economical digital microscope setup for our student compound scopes (anatomy and physiology). In lieu of getting an expensive microscope-only camera, I would like to pair a decent consumer camera with a suitable microscope mount (this way students can photograph histological sections and lab activities with the same camera). I would like to buy 3-4 setups, and stay under or around $500 each. Any ideas? Thanks, Ross Ross Langston, PhD Department of Natural Sciences Windward Community College 45-720 Keaahala Road Kaneohe, Hawaii 96744 808-236-9119 (office) 808-247-5362 (fax) From bjdewe <@t> aol.com Mon Aug 7 13:09:52 2006 From: bjdewe <@t> aol.com (bjdewe@aol.com) Date: Mon Aug 7 13:10:13 2006 Subject: [Histonet] Free IHC Workshop Message-ID: <8C88863D033051D-1134-39D1@mblk-d38.sysops.aol.com> http://www.vetmed.ucdavis.edu/vsr/dil/events.html We only have a few more seats so if you are interested please go to the website www.innvx.com and get signed up!! Cheers, Lorie Live as if you were to die tomorrow. Learn as if you were to live forever. - Gandhi - ******************************************************************* Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s)and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ******************************************************************* ________________________________________________________________________ Check out AOL.com today. Breaking news, video search, pictures, email and IM. All on demand. Always Free. From RohrT <@t> nyackhospital.org Mon Aug 7 13:35:23 2006 From: RohrT <@t> nyackhospital.org (Theresa Rohr) Date: Mon Aug 7 13:38:50 2006 Subject: [Histonet] RTU Antibodies Message-ID: Hi Histoland, I am using the Envision+ detection system from Dako and was wondering if anyone is using their RTU antibodies for Thyroglobulin, GFAP and/or CEAP. I was thinking of diluting them further since the stain is so overdone with no target retrieval and at only 10 minutes in antibody, 10 minutes in polymer and 5 minutes in DAB. Have any of you tried that?????? I would appreciate any feedback. Thanks so much, Theresa Rohr Theresa Rohr, BA, HT(ASCP) Section Head, Histology Nyack Hospital 160 North Midland Avenue Nyack, New York 10960 phone 845-348-2276 fax 845-348-8430 Nyack Hospital is required by Law to protect the privacy of health information under HIPAA Rules and Regulations Part 11, Article 45 CFR, Parts 160-164. This email, including attachments, is intended for the exclusive use of the person or the entity to which it is addressed. It may contain confidential or legally protected information. The authorized recipient is prohibited from disclosing this information to any other party and is required to destroy this information after its stated need has been fulfilled. If the recipient or reader of this email is not the intended recipient or her/his authorized agent, the reader is hereby notified that any dissemination, distribution or copying of this email is prohibited. If you believe that you have received this email in error, please advise the sender immediately by reply email and then delete this email immediately. From POWELL_SA <@t> Mercer.edu Mon Aug 7 14:47:07 2006 From: POWELL_SA <@t> Mercer.edu (Shirley Powell) Date: Mon Aug 7 14:47:36 2006 Subject: [Histonet] Digital Camera Setup for Student Microscopes In-Reply-To: Message-ID: <01M5PS8ZM08G8WW6KM@Macon2.Mercer.edu> I got my set up from Martin Microscope in Greenville SC. They make adapters for most microscopes for several cameras. My scope is an Olympus BX40 and my camera is a Sony Cybershot 5.0. This setup has served us well. Bobby Martin is a genius with digital camera/microscope setups. They can make pretty much whatever adapter you need. Their web site is http://www.martinmicroscope.com. Or you can call them and tell them what you have and what you want to do with it. I am sure Bobby would love to fly to HI to take care of your needs. The $500 may not take care of all 3 scope adapters. Martin Microscope Company 207 South Pendleton Street Easley, SC 29640 USA Phone: 864-242-3424 FAX: 864-859-3332 E-Mail: sales@martinmicroscope.com Shirley Powell -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ross Christian Langston Sent: Monday, August 07, 2006 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Digital Camera Setup for Student Microscopes Hello- I am looking for an economical digital microscope setup for our student compound scopes (anatomy and physiology). In lieu of getting an expensive microscope-only camera, I would like to pair a decent consumer camera with a suitable microscope mount (this way students can photograph histological sections and lab activities with the same camera). I would like to buy 3-4 setups, and stay under or around $500 each. Any ideas? Thanks, Ross Ross Langston, PhD Department of Natural Sciences Windward Community College 45-720 Keaahala Road Kaneohe, Hawaii 96744 808-236-9119 (office) 808-247-5362 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From froyer <@t> bitstream.net Mon Aug 7 16:04:59 2006 From: froyer <@t> bitstream.net (Ford Royer) Date: Mon Aug 7 16:05:11 2006 Subject: [Histonet] Dako Autostainer LV-1 In-Reply-To: Message-ID: <001901c6ba65$25029fb0$7701a80a@Ford> I have just received a question that I can't answer... Is the Dako "Autostainer Model# LV-1" the same model as the current Dako "Autostainer"? I checked out Dako's web site but I could not find any Model Number designations. Any help will be appreciated. Thanks! ~ Ford Ford M. Royer, MT(ASCP) Histology Product Manager Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL: 612-839-1046 Phone: 763-542-8725 Fax: 763-546-4830 eMail: froyer@bitstream.net From AnthonyH <@t> chw.edu.au Mon Aug 7 17:51:13 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Mon Aug 7 17:52:32 2006 Subject: [Histonet] Digital Camera Setup for Student Microscopes Message-ID: Look at the Nikon Coolpix. It comes with an adapter that fits down the eyepiece. We use it for all our digital photos and produces publication quality photos. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ross Christian Langston Sent: Tuesday, 8 August 2006 4:04 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Digital Camera Setup for Student Microscopes Hello- I am looking for an economical digital microscope setup for our student compound scopes (anatomy and physiology). In lieu of getting an expensive microscope-only camera, I would like to pair a decent consumer camera with a suitable microscope mount (this way students can photograph histological sections and lab activities with the same camera). I would like to buy 3-4 setups, and stay under or around $500 each. Any ideas? Thanks, Ross Ross Langston, PhD Department of Natural Sciences Windward Community College 45-720 Keaahala Road Kaneohe, Hawaii 96744 808-236-9119 (office) 808-247-5362 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From sarah <@t> kidneybiopsy.com Tue Aug 8 08:40:42 2006 From: sarah <@t> kidneybiopsy.com (Sarah Holmes) Date: Tue Aug 8 08:56:34 2006 Subject: [Histonet] not receiving all messages Message-ID: <002d01c6baf0$3e8d41c0$780a010a@wp.comcast.net> anyone else? From vazquezr <@t> ohsu.edu Tue Aug 8 09:06:21 2006 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Tue Aug 8 09:06:52 2006 Subject: [Histonet] not receiving all messages Message-ID: Sarah, You are right, I haven't been getting as much messages as usual...I guess silly me assumed that maybe a lot of people were on vacation, as I will be next two weeks. Thanks for bringing that up! Robyn OHSU From rjbuesa <@t> yahoo.com Tue Aug 8 09:21:44 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Aug 8 09:21:52 2006 Subject: [Histonet] not receiving all messages In-Reply-To: Message-ID: <20060808142144.59272.qmail@web61220.mail.yahoo.com> Robyn: The same thing happened to me some days ago. It turned out that it was a problem with my e-mail server and e-mails from Histoent had been directed to "bulk" mail. Check those before deleting the whole bunch. Ren? J. Robyn Vazquez wrote: Sarah, You are right, I haven't been getting as much messages as usual...I guess silly me assumed that maybe a lot of people were on vacation, as I will be next two weeks. Thanks for bringing that up! Robyn OHSU _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. From sarah <@t> kidneybiopsy.com Tue Aug 8 09:52:26 2006 From: sarah <@t> kidneybiopsy.com (Sarah Holmes) Date: Tue Aug 8 10:08:18 2006 Subject: [Histonet] not receiving all messages References: <002d01c6baf0$3e8d41c0$780a010a@wp.comcast.net> Message-ID: <001301c6bafa$43c7dd80$780a010a@wp.comcast.net> [Histonet] not receiving all messagesThanks for the responses. I rec'd only a spotty few from histonet and other listerves yesterday, but I'm also missing some from individuals. May be at least partially a problem on my end. May also be envy of those on vacation. Sarah ----- Original Message ----- From: Bauer, Karen To: Sarah Holmes Sent: Tuesday, August 08, 2006 10:01 AM Subject: RE: [Histonet] not receiving all messages Hi Sarah, Me too. Your email is the first I've had all morning. Very unusual... Karen Bauer Histology Supervisor Luther Hospital Eau Claire, WI ------------------------------------------------------------------------------ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Sarah Holmes Sent: Tue 8/8/2006 8:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] not receiving all messages anyone else? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************Confidentiality Notice******************** This message is intended for the sole use of the individual and entity to whom it is addressed, and may contain information that is privileged, confidential and exempt from disclosure under applicable law. Any unauthorized review, use, disclosure or distribution of this email message, including any attachment, is prohibited. If you are not the intended recipient, please advise the sender by reply email and destroy all copies of the original message. Thank you. From jtrynak <@t> hotmail.com Tue Aug 8 10:13:35 2006 From: jtrynak <@t> hotmail.com (John Rynak) Date: Tue Aug 8 10:13:43 2006 Subject: [Histonet] Wanted :Imaging Applications Specialist - Boston, MA Message-ID: Imaging Applications Specialist - Boston, MA BioView is seeking a highly professional customer focused Applications Specialist to join our Customers Support & Training team. The successful candidate will live in the Boston area and serve as a the specialist for our US and Canada clients. You will provide comprehensive training of the BioView applications to new customers, providing prompt, online, and in-field support to our users, while maintaining a high level of technical expertise in all aspects of the BioView products, including the biological, computerized and optical features of the application. Responsibilities Training of new customers to successfully operate the BioView systems Training on a variety of clinical and research applications Supporting BioView existing users by installing, fine-tuning, maintaining, and troubleshooting BioView systems and applications. Assist clients in assay / protocol transfer and development issues as they pertain to BioViews Systems and Applications Demonstrate and present new biological and technical concepts to wide audience. Design and perform experiments to investigate and solve tough technical applications problems; and preparing problem resolution reports and imparting your findings and knowledge to Product Engineers and Technical Managers. Requirements MS/Ph d in Cytogenetics, Genetics, Molecular Biology, Biology Extensive hands on experience in Cytogenetics, Flourescene In-situ Hybridization (FISH), Cell Morphology, and Immunostaining analysis. Computer experience utilizing Microsoft Office. Ability to understand computer algorithms and to tune parameters. Ability to troubleshoot and resolve technical issues. Responsible, dedicated and a quick learner. Demonstrated use of automated scientific instrumentation Ability to travel up to 75% of the time Interested candidates should forward their resume to HR.US@bioview.co.il BioView (USA) Inc. 44 Manning Road Billerica, MA 01821 BioView is a market leader in the world of FISH (fluorescent in-vitro hybridization) scanning and analysis. BioView develops, manufactures and supplies cell imaging equipment, biological kits and software to medical institutes and universities. Founded in Israel in 2000, BioView is a privately owned company that employs a team of biologists, software engineers and physicists that develop complete solutions for their clients. BioView's provides complete solution to its customers from the preparation kits, via scanning technology for morphological and fluorescent imaging, and up to final reports and presentations for physicians and lab personnel. The products are based on an outstanding cell imaging technology platform and software package that combines morphological, immuno-staining and FISH information on the same cell*. Automated, high resolution and full color staining puts morphology, immuno-staining and FISH staining on equal footing, enabling physicians and researchers to efficiently and effectively improve the quality of patient treatment and care. John Rynak BioView (USA) Inc. 44 Manning Road Billerica, MA 01821 617-645-1345m hr.us@bioview.co.il From tfranzod <@t> dal.ca Tue Aug 8 12:22:40 2006 From: tfranzod <@t> dal.ca (Tamara Franz-Odendaal) Date: Tue Aug 8 12:23:23 2006 Subject: [Histonet] digital camera set up Message-ID: <020f01c6bb0f$43536440$7220ad81@tam> We use a Sony Cybershot F707 with our microscopes. We have an adapter that fits on the phototube and connects with the camera. We can also connect the camera to the eyepiece but this means that the image you see down the microscope does not match the image the exact same field of view you see on the camera screen, which makes things difficult sometimes. Tamara Franz-Odendaal, PhD. Post-doctoral Researcher Biology Dept., Dalhousie University Halifax, NS, B3H 4J1 Canada From langston <@t> hawaii.edu Tue Aug 8 13:45:04 2006 From: langston <@t> hawaii.edu (Ross Christian Langston) Date: Tue Aug 8 13:45:15 2006 Subject: [Histonet] Re: Digital Camera Setup for Student Microscopes In-Reply-To: References: Message-ID: Thanks to everybody for their input on the camera question. Several of the responses suggested going with the Nikon Coolpix and Nikon UR-E4 adapter, so we have decided to give it a shot! Thanks Again, Ross Ross Langston, PhD Department of Natural Sciences Windward Community College 45-720 Keaahala Road Kaneohe, Hawaii 96744 808-236-9119 (office) 808-247-5362 (fax) ----- Original Message ----- From: Ross Christian Langston Date: Monday, August 7, 2006 8:03 am Subject: Digital Camera Setup for Student Microscopes To: histonet@lists.utsouthwestern.edu > Hello- I am looking for an economical digital microscope setup for > our student compound scopes (anatomy and physiology). In lieu of > getting an expensive microscope-only camera, I would like to pair > a decent consumer camera with a suitable microscope mount (this > way students can photograph histological sections and lab > activities with the same camera). I would like to buy 3-4 setups, > and stay under or around $500 each. Any ideas? > > Thanks, > > Ross > > > > Ross Langston, PhD > Department of Natural Sciences > Windward Community College > 45-720 Keaahala Road > Kaneohe, Hawaii 96744 > 808-236-9119 (office) > 808-247-5362 (fax) > From settembr <@t> umdnj.edu Tue Aug 8 14:08:39 2006 From: settembr <@t> umdnj.edu (Dana Settembre) Date: Tue Aug 8 14:09:47 2006 Subject: [Histonet] RTU Antibodies Message-ID: Hello Theresa, I have done that with GFAP and I use their LSAB2 kit, which is not as sensitive as the Envision + kit. Dana Settembre University Hospital - UMDNJ Newark, NJ >>> Theresa Rohr 08/07/06 2:35 PM >>> Hi Histoland, I am using the Envision+ detection system from Dako and was wondering if anyone is using their RTU antibodies for Thyroglobulin, GFAP and/or CEAP. I was thinking of diluting them further since the stain is so overdone with no target retrieval and at only 10 minutes in antibody, 10 minutes in polymer and 5 minutes in DAB. Have any of you tried that?????? I would appreciate any feedback. Thanks so much, Theresa Rohr Theresa Rohr, BA, HT(ASCP) Section Head, Histology Nyack Hospital 160 North Midland Avenue Nyack, New York 10960 phone 845-348-2276 fax 845-348-8430 Nyack Hospital is required by Law to protect the privacy of health information under HIPAA Rules and Regulations Part 11, Article 45 CFR, Parts 160-164. This email, including attachments, is intended for the exclusive use of the person or the entity to which it is addressed. It may contain confidential or legally protected information. The authorized recipient is prohibited from disclosing this information to any other party and is required to destroy this information after its stated need has been fulfilled. If the recipient or reader of this email is not the intended recipient or her/his authorized agent, the reader is hereby notified that any dissemination, distribution or copying of this email is prohibited. If you believe that you have received this email in error, please advise the sender immediately by reply email and then delete this email immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From esther.peters <@t> verizon.net Tue Aug 8 15:05:52 2006 From: esther.peters <@t> verizon.net (Esther Peters) Date: Tue Aug 8 15:06:03 2006 Subject: [Histonet] Re: Digital Camera Setup for Student Microscopes References: Message-ID: <44D8EEA0.3060503@verizon.net> Ross, I got adapters to attach a Nikon CoolPix 5000 to my Zeiss Student 16 trinocular microscope. It has worked fine except when using the 100x oil immersion objective. This results in a couple of bright spots on my images which are being caused by something in the camera. Microscope and adapter professional cleanings and adjustments did not help, then I borrowed a friend's CoolPix 5000 and did not have the spots problem. I had my camera and CCD professionally cleaned twice by a local Nikon repair shop with no improvement, then sent it to Nikon headquarters and uploaded images to their Web site showing the problem. It was cleaned and adjusted again, but no help and the technicians said they couldn't recreate the problem so don't know what this is. I'd asked my local shop if a technician could come to my house where the microscope is in my home office to view the problem, but no one would (maybe you'd get better service since you're in Hawaii). Needless to say, I am not entirely pleased with Nikon, especially when I know they have made at least one camera (my friend's) that does not have the problem. I guess they want me to buy another CoolPix, but cost is a concern and what if the next one I buy does have it? It is frustrating because I do a lot of oil immersion viewing and photomicrography. Hope your experience with Nikon will be better! Esther Peters, Ph.D. George Mason University Ross Christian Langston wrote: > Thanks to everybody for their input on the camera question. Several of the responses suggested going with the Nikon Coolpix and Nikon UR-E4 adapter, so we have decided to give it a shot! > > Thanks Again, > > Ross > > > Ross Langston, PhD > Department of Natural Sciences > Windward Community College > 45-720 Keaahala Road > Kaneohe, Hawaii 96744 > 808-236-9119 (office) > 808-247-5362 (fax) > > ----- Original Message ----- > From: Ross Christian Langston > Date: Monday, August 7, 2006 8:03 am > Subject: Digital Camera Setup for Student Microscopes > To: histonet@lists.utsouthwestern.edu > > >>Hello- I am looking for an economical digital microscope setup for >>our student compound scopes (anatomy and physiology). In lieu of >>getting an expensive microscope-only camera, I would like to pair >>a decent consumer camera with a suitable microscope mount (this >>way students can photograph histological sections and lab >>activities with the same camera). I would like to buy 3-4 setups, >>and stay under or around $500 each. Any ideas? >> >>Thanks, >> >>Ross >> >> >> >>Ross Langston, PhD >>Department of Natural Sciences >>Windward Community College >>45-720 Keaahala Road >>Kaneohe, Hawaii 96744 >>808-236-9119 (office) >>808-247-5362 (fax) >> >> > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From liz <@t> premierlab.com Tue Aug 8 16:31:06 2006 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Aug 8 16:26:04 2006 Subject: [Histonet] HIV control Message-ID: <000501c6bb31$f555a460$0300a8c0@domain.Premier> Does anyone out there know where I could get a HIV control block? Thanks Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 From cforster <@t> umn.edu Tue Aug 8 16:30:16 2006 From: cforster <@t> umn.edu (Colleen Forster) Date: Tue Aug 8 16:30:21 2006 Subject: [Histonet] IHC on serum smear Message-ID: <44D90268.3080701@umn.edu> Hello to all, Has anyone ever tried doing IHC on serum smears?? Colleen Forster U of MN From Sandra.Harrison3 <@t> va.gov Tue Aug 8 16:40:40 2006 From: Sandra.Harrison3 <@t> va.gov (Harrison, Sandra C.) Date: Tue Aug 8 16:40:47 2006 Subject: [Histonet] VA in Minneapolis hiring Histo techs Message-ID: <736E8889E98B8F4FBBD29FDFEC8074BA01025695@VHAV23MSGA2.v23.med.va.gov> Dear Histonetters, The Veteran's Admin. Medical Center in Minneapolis, MN., is seeking to fill a Histology vacancy. We are a teaching hospital and have many educational opportunities. The Histology lab is staffed by 6 congenial tech.'s and we range in experience level from those of us who have been in the field for 20+ years to 1 recent graduate. We don't have weekend call and do get 10 paid Holiday day's off per year. The VA's mission is to honor America's veterans by providing exceptional health care that improves their health and well-being. If you are interested in joining a team of dedicated Histotechs, please write or call 612-467-2449 for application information. Sincerely, Sandy From hmk20 <@t> hotmail.com Tue Aug 8 20:04:07 2006 From: hmk20 <@t> hotmail.com (HERNAN MOLINA-KIRSCH) Date: Tue Aug 8 20:04:14 2006 Subject: [Histonet] Digital Camera Setup for Student Microscopes Message-ID: My advice to you is to go with coolpix 995 or 4500 and you can get an exelent coupler from ebay for less than $100 [1]http://cgi.ebay.com/NIKON-CP-CAMERA-LENS-ADAPTER-FOR-TRINOCULAR-MIC ROSCOPE_W0QQitemZ260015814506QQihZ016QQcategoryZ11813QQrdZ1QQcmdZViewI tem. Both cameras are cientific and relatibly unexpensive but you will be able to get 100% focus from 1x t0 100x objectives with real minor adjustments a very important point which usually you are unable to get from cameras with more megapixels but less reliable optics. Please see what I have been doing for years with mi nikon labophot 2 with plan or plan apo optics at the following address: [2]http://pat.uninet.edu/zope/pat/casos/C240/index.html [3]http://pat.uninet.edu/zope/pat/casos/C234/index.html LABORATORIO DE PATOLOGIA Dr. Hernan Molina Kirsch 3ra. Calle 9-78, Zona 1. 01001 Apartado Postal # 2767 Ciudad de Guatemala, Guatemala, C. A. Phones: (5022) 2536056 2517008 2517009 Fax (502) 2380436 LABORATORIO DE PATOLOGIA Dr. Hernan Molina Kirsch 3ra. Calle 9-78, Zona 1. 01001 Apartado Postal # 2767 Ciudad de Guatemala, Guatemala, C. A. Phones: (5022) 2536056 2517008 2517009 Fax (502) 2380436 _________________________________________________________________ Express yourself instantly with MSN Messenger! [4]MSN Messenger Download today it's FREE! References 1. http://cgi.ebay.com/NIKON-CP-CAMERA-LENS-ADAPTER-FOR-TRINOCULAR-MICROSCOPE_W0QQitemZ260015814506QQihZ016QQcategoryZ11813QQrdZ1QQcmdZViewItem 2. http://pat.uninet.edu/zope/pat/casos/C240/index.html 3. http://pat.uninet.edu/zope/pat/casos/C234/index.html 4. http://g.msn.com/8HMAEN/2731??PS=47575 From Karen.Heckford <@t> CHW.edu Wed Aug 9 09:03:40 2006 From: Karen.Heckford <@t> CHW.edu (Heckford, Karen - SMMC-SF) Date: Wed Aug 9 09:05:42 2006 Subject: [Histonet] Setting Ink Message-ID: Cheers Everyone, Does anyone know if there is something that sets the ink on tissue after you have inked the margins. I vaguely remember something but I cannot seemed to dig it out of my brain. Thanks in advance, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathololgy Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu From MSHERWOOD <@t> PARTNERS.ORG Wed Aug 9 09:13:33 2006 From: MSHERWOOD <@t> PARTNERS.ORG (Sherwood, Margaret ) Date: Wed Aug 9 09:13:39 2006 Subject: [Histonet] Setting Ink Message-ID: <1AF23D0AD12E7444A5DB083CA978B73407A30B4F@PHSXMB1.partners.org> We use TMD (tissue marking dye)from Triangle Biomedical Sciences, Inc. Fisher Scientific carries it--sold in a pack of 5-6 colors. Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Heckford, Karen - SMMC-SF Sent: Wednesday, August 09, 2006 10:04 AM To: Histonet (E-mail) Subject: [Histonet] Setting Ink Cheers Everyone, Does anyone know if there is something that sets the ink on tissue after you have inked the margins. I vaguely remember something but I cannot seemed to dig it out of my brain. Thanks in advance, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathololgy Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu From Lynne.Bell <@t> hitchcock.org Wed Aug 9 09:21:52 2006 From: Lynne.Bell <@t> hitchcock.org (Bell, Lynne) Date: Wed Aug 9 09:21:56 2006 Subject: [Histonet] Setting Ink Message-ID: Karen, We use a 3% acetic acid solution to set the ink. In the past we had used Bouin's solution, but couldn't stand the smell or the mess. The acetic acid solution works great. Lynne Bell, HT (ASCP) Lead Histology Technician Central Vermont Medical Center Barre, VT 05641 802-371-4923 From vazquezr <@t> ohsu.edu Wed Aug 9 09:26:49 2006 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Wed Aug 9 09:27:21 2006 Subject: [Histonet] Setting Ink Message-ID: Karen, If my memory serves me correctly, it would be to dip the tissue in 95% alc and then dab carefully dry to get excess alc off. Robyn OHSU From wilson_jm <@t> cimar.org Wed Aug 9 09:31:02 2006 From: wilson_jm <@t> cimar.org (Jonathan Wilson) Date: Wed Aug 9 09:30:51 2006 Subject: [Histonet] DAPI intensity differences In-Reply-To: <6.1.1.1.2.20060807105758.019c65d0@mail.vet.upenn.edu> Message-ID: Hello, I have done some nuclear staining with DAPI and I have found that the intensity is changing with my treatment. I know that DAPI will stain both DNA/RNA and that different cell types will sometimes have differences in staining intensity but has anyone observed changes within the same cell type. I couldn't find anything in the archives and my pubmed searches weren't too enlightening. Any input would be greatly appreciated. Sincerely, Jon Jonathan Mark Wilson (PhD) CIIMAR-Ecofisiologia Rua dos Bragas 289 4050-123 Porto, Portugal tel 351 22 340 1809 fax 351 22 339 0608 alt email: jmwilson@ciimar.up.pt -----Original Message----- From: Pamela Marcum [mailto:pmarcum@vet.upenn.edu] Sent: segunda-feira, 7 de Agosto de 2006 16:18 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Pennsylvaina Fall Symposium October, 2006 We in the Pennsylvaina Histotechnology Society are proud to announce the opening of our web site at www.pahisto.org and the program for our October. 2006 meeting in Pittsburgh. We will be adding to the site and updating all areas of the program to include our vendors very soon. The program is currently not in pdf. and we apologize. PHS felt it was more important to get the program out for review than to hold for several more weeks while we are setting up. The current program is in Microsoft Word and can be printed off for those who need now. We hope this helps you decide to join us for one or all of the three days. PHS is using the Thursday for those who need more information on CPT Coding, CAP Inspections and Ergonomics for laboratories and supervisors. The class size is not limited to PHS, constituent state societies or NSH members. These seminars are being run over the course of one day with not competition from other seminars to allow those wishing to come and not miss more technical or specialized seminars. If you have a laboratory manager who is not in the histology department and still needs to understand CPT codes or CAP inspections for Histology this will be a good place to learn and talk to others struggling (not that any of us do) with these areas of laboratory management. The seminars on Friday and Saturday will be more technical and some just plain fun. We will have a lecture on the Bog People of Nothern Europe (mummies) on Friday. Special hands on with Mohs grossing and sectioning and then just frozen sectioning for the routine clinical area later. Want to know about grossing for the histologist we have a seminar for that too. We attempted to talk the Dr. Gore into leaving his title on DNA, PCR as ISHY, FISHY, PCR and he declined. It is on Friday afternoon. The IHC talks are for those starting who still have questions (Friday morning) and those more advanced with double and triple staining (Saturday morning). Saturday will also have a room devoted to CSI type lectures. Check the web site www.pahisto.org and join us for one or all three days. Thanks to LabVision for setting the basis of the web site and letting us fill in the blanks. Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMahoney <@t> alegent.org Wed Aug 9 09:46:34 2006 From: JMahoney <@t> alegent.org (Janice Mahoney) Date: Wed Aug 9 09:46:55 2006 Subject: [Histonet] Setting Ink Message-ID: Bouin's Fixative will set ink. Jan Omaha >>> "Sherwood, Margaret " 08/09/2006 9:13 AM >>> We use TMD (tissue marking dye)from Triangle Biomedical Sciences, Inc. Fisher Scientific carries it--sold in a pack of 5-6 colors. Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (W224) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-3192 (fax) msherwood@partners.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Heckford, Karen - SMMC-SF Sent: Wednesday, August 09, 2006 10:04 AM To: Histonet (E-mail) Subject: [Histonet] Setting Ink Cheers Everyone, Does anyone know if there is something that sets the ink on tissue after you have inked the margins. I vaguely remember something but I cannot seemed to dig it out of my brain. Thanks in advance, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathololgy Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From alaskagirl1950 <@t> yahoo.com Wed Aug 9 09:59:17 2006 From: alaskagirl1950 <@t> yahoo.com (Patricia Adams) Date: Wed Aug 9 09:59:23 2006 Subject: [Histonet] my first time sending to HistoNet Message-ID: <20060809145917.35481.qmail@web52503.mail.yahoo.com> I just wanted to see if I have this right! Patricia Adams HT (ASCP) Tuskegee Universtiy Tuskegee, Alabama Patricia Adams ----- Fight back spam! Download the Blue Frog. http://www.bluesecurity.com/register/s?user=YWxhc2thZ2lybDE5NTA%3D __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From Nancy.Temple <@t> ssfhs.org Wed Aug 9 10:01:12 2006 From: Nancy.Temple <@t> ssfhs.org (Temple Nancy) Date: Wed Aug 9 10:01:31 2006 Subject: [Histonet] Re: Setting Ink Message-ID: We use White Vinegar for setting ink. We use India Ink and also Tissue Marking Dyes from Cancer Diagnostics.The vinegar works just fine. Nancy Temple HT(ASCP) Supervisor Histology/Cytology St. Francis Hospital Centers Indianapolis, In 46237 From LuckG <@t> empirehealth.org Wed Aug 9 10:06:11 2006 From: LuckG <@t> empirehealth.org (Luck, Greg D.) Date: Wed Aug 9 10:06:19 2006 Subject: [Histonet] Setting Ink Message-ID: <6BB8BC4519AAB844B174FC739A679BBC508ED1@IRMEXCH01.irm.inhs.org> Karen, As already mentioned Bouin's (is best), acetic acid is acceptable but another alternative I discovered is a diluted Bouin's in a spray bottle configuration (named INK+AID) from a company called "Cancer Diagnostics". It's very neat, tidy, goes a long way and has become very popular here. Here's a weblink for the company: www.cancerdiagnostics.com. Good luck, Greg Greg Luck Anatomic Pathology Supervisor Deaconess Med Cntr 800 W. 5th Ave Spokane, WA 99204 Offc 509.473.7077 Fax 509.473.7133 luckg@empirehealth.org www.deaconessmc.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Wednesday, August 09, 2006 7:04 AM To: Histonet (E-mail) Subject: [Histonet] Setting Ink Cheers Everyone, Does anyone know if there is something that sets the ink on tissue after you have inked the margins. I vaguely remember something but I cannot seemed to dig it out of my brain. Thanks in advance, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathololgy Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu From sheila_adey <@t> hotmail.com Wed Aug 9 12:22:43 2006 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Wed Aug 9 12:22:52 2006 Subject: [Histonet] Cryostat disinfectant Message-ID: Hello histonetters, I recall reading about a cryostat disinfectant that could be used with out defrosting the unit? If you have any info on such a product, I would appreciate it. Sheila Adey HT Port Huron Hospital Michigan _________________________________________________________________ Play Q6 for your chance to WIN great prizes. http://q6trivia.imagine-live.com/enca/landing From RSRICHMOND <@t> aol.com Wed Aug 9 12:29:55 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Wed Aug 9 12:30:10 2006 Subject: [Histonet] Re: Setting Ink Message-ID: <383.8a7ce5d.320b7593@aol.com> Karen Heckford HT (ASCP) CE, Lead Histology Technician, Histology/Pathology Department, St. Mary's Medical Center, San Francisco CA asks: >>Does anyone know if there is something that sets the ink on tissue after you have inked the margins?<< I rarely use anything to set the ink when I gross surgical specimens. the important thing is to blot the specimen dry before you put the ink on. Bouin's solution, and acetone, are widely used but are environmentally unacceptable. 2% to 3% acetic acid is supposed to work quite satisfactorily, but I don't think I've ever tried it. Bob Richmond Knoxville TN and Gastonia NC From Michael.Rice <@t> holy-cross.com Wed Aug 9 12:43:59 2006 From: Michael.Rice <@t> holy-cross.com (Rice, Michael) Date: Wed Aug 9 12:44:07 2006 Subject: [Histonet] setting ink Message-ID: <3BC92F29BE821745AB15E04C98EE028D020FCA1E@HCH2KMAIL.holy-cross.com> We use straight acetone to set ink, it works with all of our colors from Davidson Michael Rice CT.HT(ASCP) Supervisor Of Pathology Holy Cross Hospital Ft Lauderdale, Fl 33308 954.776.3070 ====================== Confidentiality Notice: Prepared in Anticipation of Litigation. Attorney-Client Privileged. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original. ====================== From Kari.Zajic <@t> HCAhealthcare.com Wed Aug 9 12:46:25 2006 From: Kari.Zajic <@t> HCAhealthcare.com (Zajic Kari) Date: Wed Aug 9 12:46:29 2006 Subject: [Histonet] Job Postings-Florida Message-ID: <095327C7CDBDF64B9E9728A54799091E015CC5C0@ORLEV03.hca.corpad.net> Does anyone know of a good resource for Histology job postings in South Florida aside from Advance?? Please, not interested in "head hunters" or "placement" facilities.... Thank you. Kari Marie Zajic HT,MLT Histology Supervisor Palms West Hospital Pathology Department 13001 State Road Eighty Loxahatchee, Florida 33470 phone: (561)798-6036 telefax: (561)753-4298 voicemail: (561)753-4299 pager: (561)610-4949 email: Kari.Zajic@HCAHealthcare.com This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. From rjbuesa <@t> yahoo.com Wed Aug 9 14:15:29 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Aug 9 14:15:35 2006 Subject: [Histonet] Job Postings-Florida In-Reply-To: <095327C7CDBDF64B9E9728A54799091E015CC5C0@ORLEV03.hca.corpad.net> Message-ID: <20060809191529.36772.qmail@web61218.mail.yahoo.com> Try "Monster" Ren? J. Zajic Kari wrote: Does anyone know of a good resource for Histology job postings in South Florida aside from Advance?? Please, not interested in "head hunters" or "placement" facilities.... Thank you. Kari Marie Zajic HT,MLT Histology Supervisor Palms West Hospital Pathology Department 13001 State Road Eighty Loxahatchee, Florida 33470 phone: (561)798-6036 telefax: (561)753-4298 voicemail: (561)753-4299 pager: (561)610-4949 email: Kari.Zajic@HCAHealthcare.com This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Next-gen email? Have it all with the all-new Yahoo! Mail Beta. From escott8 <@t> houston.rr.com Wed Aug 9 14:21:54 2006 From: escott8 <@t> houston.rr.com (Edward Scott) Date: Wed Aug 9 14:21:47 2006 Subject: [Histonet] Breast Processing Message-ID: <002901c6bbe9$15b5d510$6500a8c0@thescotts> Can someone share their breast tissue processing protocol with me. We are currently fixing the breast overnight in rapid fixx solution and putting it into acetone the next day,and then it is processed overnight. The slides usually come out the third day. The pathologist want a faster turnaround time. The sections uusually come out good. It also depends on how thick the residents make the sections. I don't use alcoholic formalin on my processor. Any help in this matter would be greatly appreciated. Allison Scott HT(ASCP) Histology Supervior LBJ Hospital Houston, Texas 77026 From rhessler <@t> pathgroup.com Wed Aug 9 15:33:44 2006 From: rhessler <@t> pathgroup.com (Richard Hessler, M.D.) Date: Wed Aug 9 15:33:47 2006 Subject: [Histonet] Ventana PanCK-Cross reacts with GFAP Message-ID: This cocktail seems to be a mix of AE1/AE3 and one that I am not familiar with-PCK26. After years of Dako I am now using Ventana for the first time and was shocked to see 4+ staining of reactive gliosis with this Cocktail. Has anyone seen this? Why do they not use just AE1/AE3 like everyone else? But, most importantly, how is this stuff "approved for in vitro diagnostic use"??? --------------------------------------------------------------------- Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc. immediately at 615-562-9255. From Rcartun <@t> harthosp.org Wed Aug 9 15:49:11 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Aug 9 15:49:47 2006 Subject: [Histonet] Ventana PanCK-Cross reacts with GFAP In-Reply-To: References: Message-ID: <44DA12070200007700001522@hcnwgwds01.hh.chs> Cross-reactivity of GFAP with low molecular weight cytokeratin antibodies (e.g., CAM5.2 and others) has been well documented over the years. A sensitive, but epithelial-specific (does not cross-react with GFAP) cytokeratin monoclonal is OSCAR (developed by Dr. Allen Gown) available from Signet Laboratories. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Richard Hessler, M.D." 08/09/06 4:33 PM >>> This cocktail seems to be a mix of AE1/AE3 and one that I am not familiar with-PCK26. After years of Dako I am now using Ventana for the first time and was shocked to see 4+ staining of reactive gliosis with this Cocktail. Has anyone seen this? Why do they not use just AE1/AE3 like everyone else? But, most importantly, how is this stuff "approved for in vitro diagnostic use"??? --------------------------------------------------------------------- Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc. immediately at 615-562-9255. From abardo <@t> mcbaininstruments.com Wed Aug 9 16:03:10 2006 From: abardo <@t> mcbaininstruments.com (Ann Bardo) Date: Wed Aug 9 16:03:16 2006 Subject: [Histonet] Job Posting - S. Calif Message-ID: <20060809140346.SM01560@WSAnn> McBain Instruments posted a job opening last week on this site for a Histology Sales Specialist in Southern California. I'd welcome any suggestions you all may have for other resources for candidates for the position. Ann Bardo McBain Instruments 818-998-2702 www.mcbaininstruments.com From danarobinmarshal <@t> aol.com Wed Aug 9 16:18:01 2006 From: danarobinmarshal <@t> aol.com (danarobinmarshal@aol.com) Date: Wed Aug 9 16:18:19 2006 Subject: [Histonet] RNA later Message-ID: <8C88A106E18BB08-E60-177E@FWM-M07.sysops.aol.com> Hi everyone, There was a thread earlier this year involving treatment of RNA-later stored tissue such that it can be embedded and cut without fracturing. I found one answer on the archives referring to a quick cold DEPC water rinse prior to OCT. Does anyone else have any advice as to how they have handled this? Thanks, dana ________________________________________________________________________ Check out AOL.com today. Breaking news, video search, pictures, email and IM. All on demand. Always Free. From antyler <@t> uncg.edu Wed Aug 9 18:16:00 2006 From: antyler <@t> uncg.edu (Amber Tyler ANTYLER) Date: Wed Aug 9 18:15:45 2006 Subject: [Histonet] Long term storage of mouse brains Message-ID: Hi, I'm just beginning to learn histochemical techniques. I will have a number of adult mouse brains I would like to store for about a year. What is the best way to do this without causing tissue damage? As of right now, I was thinking of fixing in 2-methyl-butane/dry ice then storing at -80C. I would like to eventually do Autoradiography and/or Immunohistochemistry staining in the hypothalamus and hippocampus. Thank you for any advice and information you can provide. Amber N. Tyler, Ph.D. Research Scientist University of North Carolina - Greensboro Greensboro, NC 27402 Office: 336.334.4046 antyler@uncg.edu From AnthonyH <@t> chw.edu.au Wed Aug 9 18:16:47 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Wed Aug 9 18:18:06 2006 Subject: [Histonet] RNA later Message-ID: A quick rinse in water is good, followed by an hour or so infiltration with OCT solution (about a 30-60% solution in water) should improve the cryotomy. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of danarobinmarshal@aol.com Sent: Thursday, 10 August 2006 7:18 AM To: histonet@lists.utsouthwestern.edu Cc: dkhabele@mmc.edu Subject: [Histonet] RNA later Hi everyone, There was a thread earlier this year involving treatment of RNA-later stored tissue such that it can be embedded and cut without fracturing. I found one answer on the archives referring to a quick cold DEPC water rinse prior to OCT. Does anyone else have any advice as to how they have handled this? Thanks, dana ________________________________________________________________________ Check out AOL.com today. Breaking news, video search, pictures, email and IM. All on demand. Always Free. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From dr.vinod.kr <@t> gmail.com Wed Aug 9 22:54:20 2006 From: dr.vinod.kr <@t> gmail.com (K.R. Vinod) Date: Wed Aug 9 22:54:30 2006 Subject: [Histonet] PFA v. formalin Message-ID: Hello Melissa Usually, tissues are fixed in 4% PFA (Paraformaldehyde) for 3 to 4 hours at 4 ?C. 10% formalin in PBS can also be used. Tissues fixed for long time in formalin may not give good result. However, it depends on what type of tissue you are using. K.R. Vinod, Ph.D. Glenmark Research Centre, New Bombay. From orit.kollet <@t> weizmann.ac.il Thu Aug 10 00:29:42 2006 From: orit.kollet <@t> weizmann.ac.il (orit kollet) Date: Thu Aug 10 00:29:50 2006 Subject: [Histonet] Bone section double staining for immunohistochemistry Message-ID: Hello Histonet members, Does anyone have an experience with immunohistochemistry double or triple staining for mouse bones, formalin fixed, EDTA decalcified and paraffin embedded? Your tips are appreciated! Best, Orit Orit Kollet Ph.D. Weizmann Institute of Science Department of Immunology Rehovot 76100 Israel Orit.Kollet@weizmann.ac.il Tel: 972-(0)8-9342784 Fax: 972-(0)8-9344141 From Mary.Judd <@t> suht.swest.nhs.uk Thu Aug 10 03:44:50 2006 From: Mary.Judd <@t> suht.swest.nhs.uk (Mary Judd) Date: Thu Aug 10 03:45:05 2006 Subject: [Histonet] Not recieving all of Histonet Message-ID: I have noticed that when there is a lot of correspondence on Histonet I don't recieve all of them.If there are 20 topics listed I can only view about 12 of them. Mary Judd Mary Judd Section Head Immunohistochemistry Cellular Pathology Level E, Mailpoint 2 Southampton University Hospitals Trust Tel. 02380 795144 Fax. 02380 796869 D I S C L A I M E R This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Trust unless explicitly stated otherwise. If you have received this e-mail in error please delete the e-mail and contact the Southampton University Hospitals NHS Trust Helpdesk on:- 023 80796000 The information contained in this e-mail may be subject to public disclosure under the Freedom of Information Act 2000. Unless the Information is legally exempt from disclosure, the confidentiality of this e-mail and your reply cannot be guaranteed. This footnote also confirms that this email message has been swept by MailMarshal for the presence of computer viruses. Please visit our website at http://www.suht.nhs.uk From Barry.R.Rittman <@t> uth.tmc.edu Thu Aug 10 06:08:11 2006 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Thu Aug 10 06:08:15 2006 Subject: [Histonet] PFA v. formalin Message-ID: There has been a lot of discusion re formalin versus paraformaldehyde ad concentrations. In most cases it will not make a lot of difference whether PFA or formlin are used. I am concerned that in the rush to use paraformaldehyde instead of formalin because of purity concerns may result in more accidents when heating the solution to make the paraformaldehyde go into solution. If you are concerned with the purity of formalin then purchase the closed vials of formalin that are available from several manufacturers and that can be stored for a reasonable long time. First however I would run tests to determine whether the formalin that you are using is really unsuitable. Standardization is important. Similarly I believe that the concern for a ensuring that frormalin used be within very narrow concentration limits is also somewhat of an overreaction. The fixation process relies on availability of formalin and time of fixation etc. Concentration within few percentage points is not that important. Barry ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of K.R. Vinod Sent: Wed 8/9/2006 10:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] PFA v. formalin Hello Melissa Usually, tissues are fixed in 4% PFA (Paraformaldehyde) for 3 to 4 hours at 4 ?C. 10% formalin in PBS can also be used. Tissues fixed for long time in formalin may not give good result. However, it depends on what type of tissue you are using. K.R. Vinod, Ph.D. Glenmark Research Centre, New Bombay. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From j.potas <@t> gmail.com Thu Aug 10 08:23:24 2006 From: j.potas <@t> gmail.com (Jason Potas) Date: Thu Aug 10 08:23:30 2006 Subject: [Histonet] storing frozen sections on glass slides in the freezer Message-ID: Hi everyone/anyone, I would like to store frozen rat spinal cord sections in a -20 degree C freezer. Does anyone have experience with the following method, ie does it work: 1-cut sections frozen onto gelatinized glass slide. 2-air dry overnight at room temperature (to remove all moisture) 3-wrap multiple glass slides inside aluminium foil together like a pack of chewing gum 4-store in the freezer at -20 deg C 5-when required, removed one (or required number) slide(s) from the pack, but without defrosting other slides 6-allow to reach room temperature and commence histochemistry as normal does it work without stuffing up the slides or the tissue sticking on the back of other slides. has someone done this? cheers jason -- ______________________________________ Jason Potas (PhD) Laboratorio de Neurobiologia Celular e Molecular Instituto de Biofisica Carlos Chagas Filho - IBCCF Universidade Federal do Rio de Janeiro CCS bloco J sala J1-029 Ilha do Fundao 21941-590 Rio de Janeiro, RJ - Brasil Tel: +5521 2280 4694 +5521 2562 6554 Email: jason@biof.ufrj.br From mcauliff <@t> umdnj.edu Thu Aug 10 09:09:15 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Aug 10 09:08:34 2006 Subject: [Histonet] storing frozen sections on glass slides in the freezer In-Reply-To: References: Message-ID: <44DB3E0B.4020509@umdnj.edu> Hi Jason: First off, I would not store, do the reaction asap after cutting the sections. If you must store, I would store at -80C. You may still get some reduction in the reaction, depending on what you are looking for and how long the storage is. Geoff Jason Potas wrote: > Hi everyone/anyone, > > I would like to store frozen rat spinal cord sections in a -20 degree C > freezer. Does anyone have experience with the following method, ie > does it > work: > > 1-cut sections frozen onto gelatinized glass slide. > 2-air dry overnight at room temperature (to remove all moisture) > 3-wrap multiple glass slides inside aluminium foil together like a > pack of > chewing gum > 4-store in the freezer at -20 deg C > 5-when required, removed one (or required number) slide(s) from the pack, > but without defrosting other slides > 6-allow to reach room temperature and commence histochemistry as normal > > does it work without stuffing up the slides or the tissue sticking on the > back of other slides. has someone done this? > > cheers > jason > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From jennifer.l.hofecker <@t> Vanderbilt.Edu Thu Aug 10 09:53:26 2006 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Thu Aug 10 09:53:31 2006 Subject: [Histonet] storing frozen sections on glass slides in the freezer References: Message-ID: <898D946569A27444B65667A49C074052852E64@mailbe06.mc.vanderbilt.edu> Hi Jason, We do cut and store our frozen muscle slides in the freezer. Our protocol is a little different that yours, but I will share anyway... We cut our frozen sections of muscle at 8 microns and place on positive charged slides. Sections are circled with a hydrophobic pen and placed in a slide box (one hundred slide plastic box). The box is then placed in the -70 freezer. We cut a few extra sections beyond what we need for our histochemistry panel and also store those in a slide box in the freezer. We have gone back and pulled extra slides up to a few months later, and the enzyme histochems worked fine. We also keep our normal muscle controls in a slide box at -70 and we pull them out as needed. Again, no problems encountered with the staining. As you pointed out in your post, be sure to allow slides to come to room temp before staining (we learned that the hard way). I realize that we're talking different tissue and different slides, but I wanted to let you know that we do this very successfully. The main difference in our protocols that I would worry about is the -20 freezer. Does it cycle through defrost everynight? If it does, you could still use it, just pack any extra space around the slides with cold packs. We've done that from time to time when we couldn't get things into the -70 the cold packs keep the temps fairly stable. Not sure about the "chewing gum" method either. Let us know how that goes... Thanks and Good luck. Jennifer Hofecker, HT (ASCP) Vanderbilt University Medical Center Division of Neuropathology (615) 343-0083 (615) 343-7089 fax ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Jason Potas Sent: Thu 8/10/2006 8:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] storing frozen sections on glass slides in the freezer Hi everyone/anyone, I would like to store frozen rat spinal cord sections in a -20 degree C freezer. Does anyone have experience with the following method, ie does it work: 1-cut sections frozen onto gelatinized glass slide. 2-air dry overnight at room temperature (to remove all moisture) 3-wrap multiple glass slides inside aluminium foil together like a pack of chewing gum 4-store in the freezer at -20 deg C 5-when required, removed one (or required number) slide(s) from the pack, but without defrosting other slides 6-allow to reach room temperature and commence histochemistry as normal does it work without stuffing up the slides or the tissue sticking on the back of other slides. has someone done this? cheers jason -- ______________________________________ Jason Potas (PhD) Laboratorio de Neurobiologia Celular e Molecular Instituto de Biofisica Carlos Chagas Filho - IBCCF Universidade Federal do Rio de Janeiro CCS bloco J sala J1-029 Ilha do Fundao 21941-590 Rio de Janeiro, RJ - Brasil Tel: +5521 2280 4694 +5521 2562 6554 Email: jason@biof.ufrj.br _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From froyer <@t> bitstream.net Thu Aug 10 10:05:48 2006 From: froyer <@t> bitstream.net (Ford Royer) Date: Thu Aug 10 10:06:18 2006 Subject: [Histonet] Linear Stainer In-Reply-To: Message-ID: <002a01c6bc8e$80e929e0$7701a80a@Ford> I'm looking for a used Leica ST-4040 Linear Stainer. Please contact me off-list if you have one that you would like to sell. Thanks, ~ Ford Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL: 612-839-1046 Phone: 763-542-8725 Fax: 763-546-4830 eMail: froyer@bitstream.net From Susan.Walzer <@t> HCAHealthcare.com Thu Aug 10 10:28:21 2006 From: Susan.Walzer <@t> HCAHealthcare.com (Walzer Susan) Date: Thu Aug 10 10:28:31 2006 Subject: [Histonet] Setting Ink In-Reply-To: Message-ID: <471953BC63077941B82C26A4338272B42F04A2@ORLEV03.hca.corpad.net> SOME OF OUR DOCS LIKE ACETONE SOME LIKE BOUINS -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Heckford, Karen - SMMC-SF Sent: Wednesday, August 09, 2006 10:04 AM To: Histonet (E-mail) Subject: [Histonet] Setting Ink Cheers Everyone, Does anyone know if there is something that sets the ink on tissue after you have inked the margins. I vaguely remember something but I cannot seemed to dig it out of my brain. Thanks in advance, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathololgy Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu From LRaff <@t> lab.uropartners.com Thu Aug 10 12:04:15 2006 From: LRaff <@t> lab.uropartners.com (Lester Raff) Date: Thu Aug 10 12:04:24 2006 Subject: [Histonet] CAP question Message-ID: <5DA1CA5D0B98A84985B545A24423B8220198E2@UPLAB01.uplab.local> How do all of you comply with the following? For laboratories subject to US federal regulations, do all testing personnel meet CLIA?88 requirements? NOTE: There must be evidence in personnel records that all testing personnel have been evaluated against CLIA?88 requirements, and that all individuals qualify. Thanks, Lester J. Raff, MD Medical Director UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL 60154 ph: 708-486-0076 fax: 708-486-0080 From vanann702 <@t> skmc.gov.ae Thu Aug 10 12:04:56 2006 From: vanann702 <@t> skmc.gov.ae (Anne Van Binsbergen) Date: Thu Aug 10 12:05:01 2006 Subject: [Histonet] Renals - FITC Message-ID: Hello Histonetters some informtion required regarding Renals/FITC: what are the optimum storage requirements for STAINDED slides - time, temp etc? we are trying to repatriate these tests (currently sent out) but we have the method up and running in a parallel study and just need to iron out a few small 'crinkles' there is confusion among the pathologists regarding the 'lifespan' of the fluorescence all advice/assistance welcome thanks Annie From vanann702 <@t> skmc.gov.ae Thu Aug 10 12:16:57 2006 From: vanann702 <@t> skmc.gov.ae (Anne Van Binsbergen) Date: Thu Aug 10 12:16:53 2006 Subject: [Histonet] paraffin wax Message-ID: another quick question i was taught that it is not a good idea to leave tissue in molten wax for extended periods of time - my rule of thumb has always been 4 hours max for an 'average' size tissue and half of that for a very small biopsy. we have a VIP5 and a very important sample (lymph node) has been left in the machine for over 7 hours before embedding, by a 'tardy' tech. wax baths set to 59 celcius. i am seriously concerned that the tissue could have been damaged by prolonged exposure to heat. any comments - or am i being paranoid :-) annie From pmarcum <@t> vet.upenn.edu Thu Aug 10 12:29:04 2006 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Thu Aug 10 12:29:10 2006 Subject: [Histonet] Pennsylvania Newsletter on line Message-ID: <6.1.1.1.2.20060810132633.01998088@mail.vet.upenn.edu> Good Afternoon, We have just added the PHS Newsletter to our web site. It is the fastest way to get information to our members on line. We have a contest to name the state newsletter to please let us know your suggestions. The web site is www.pahisto.org. Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From PMonfils <@t> Lifespan.org Thu Aug 10 13:44:50 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Aug 10 13:44:55 2006 Subject: [Histonet] paraffin wax Message-ID: <09C945920A6B654199F7A58A1D7D1FDE0171777C@lsexch.lsmaster.lifespan.org> Yes, we do want to avoid "slow cooking" our tissue samples by extended exposure to heat. However, 7 hours at 59 degrees is not likely to cause any major problems for an average size specimen. It could cause problems for a small biopsy! You can tell when you section it. If it sections normally, it should look normal microscopically. If it doesn't section as well as it should, soaking the faced block in water for 15 to 30 minutes before sectioning should help. From JSCHUMA1 <@t> Fairview.org Thu Aug 10 14:27:40 2006 From: JSCHUMA1 <@t> Fairview.org (Schumacher, Jennifer J) Date: Thu Aug 10 14:27:47 2006 Subject: [Histonet] RE: NFP Message-ID: I am posting this for a colleague, please include her address in your replies. Thank you. Jennifer ________________________________ From: Maruska, Ann L Sent: Thursday, August 10, 2006 2:24 PM To: Schumacher, Jennifer J Subject: NFP Hi Histonetters, I have had a difficult time find a good neurofilament protein to run on our Ventana XT stainer. The problem is usually nonspecific staining. Could someone please recommend an antibody/protocol they are using for this IHC stain? TIA Ann Ann Maruska Lead IHC tech University of Minnesota Medical Center, Fairview 612-273-9119 amarusk1@fairview.org From lanbergld <@t> vcu.edu Thu Aug 10 17:21:17 2006 From: lanbergld <@t> vcu.edu (Lawrence D Lanberg/O/VCU) Date: Thu Aug 10 17:21:23 2006 Subject: [Histonet] How to Embed in High-humidity env. (???) Message-ID: Dear Histonetters, I embed mouse eyeballs in Spurr's Formula (epoxide), after dehydrating them in a series of increasing EtOh strengths. I section these with a glass knife, 0.99 micron thick. I am hoping someone very familiar with Spurr's can help. Beginning very recently, the blocks are poorly cured in the central cavity of the eye (outside is ok). This problem began with the onset of summer; prolonged baking does not help. I've even tried a 'rapid cure' modification -- more than doubling the DMAE accelerator. Our best guess is that environmental moisture is the culprit. I understand that epoxides do not work well in humidity. Unforutnately, I'm in Richmond Virginia, where one can literally poach an egg by holding it up in the air. The humidity goes right through air-conditioned walls. Does anyone know of some modifications I can make, to where my Spurrr / epoxide embedding will be successful in the high-humidity environment? I would be very grateful. Larry Lanberg From kfineout <@t> hotmail.com Thu Aug 10 20:18:05 2006 From: kfineout <@t> hotmail.com (Kelly Larson) Date: Thu Aug 10 20:18:14 2006 Subject: [Histonet] Prefilled formalin containers Message-ID: I use Fisher, which has prefilled 40/20 formalin containers (and other sizes) individually wrapped in groups of 20 (or 25, I can't remember now). This comes in handy for distribution to offices. I don't have any problem with leaking lids unless the clients don't screw them on tight. I have never had them leak before sending them out. From BMolinari <@t> heart.thi.tmc.edu Fri Aug 11 05:43:42 2006 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Aug 11 05:43:48 2006 Subject: [Histonet] paraffin wax In-Reply-To: Message-ID: We do not leave our blocks in molten paraffin. If the blocks cannot be embedded ASAP I take them out and let them cool allowing the paraffin to harden around the tissue. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Anne Van Binsbergen Sent: Thursday, August 10, 2006 12:17 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] paraffin wax another quick question i was taught that it is not a good idea to leave tissue in molten wax for extended periods of time - my rule of thumb has always been 4 hours max for an 'average' size tissue and half of that for a very small biopsy. we have a VIP5 and a very important sample (lymph node) has been left in the machine for over 7 hours before embedding, by a 'tardy' tech. wax baths set to 59 celcius. i am seriously concerned that the tissue could have been damaged by prolonged exposure to heat. any comments - or am i being paranoid :-) annie _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From christiegowan <@t> msn.com Fri Aug 11 08:24:24 2006 From: christiegowan <@t> msn.com (CHRISTIE GOWAN) Date: Fri Aug 11 08:24:34 2006 Subject: [Histonet] How to Embed in High-humidity env. (???) In-Reply-To: Message-ID: Larry, I would suggest curing your blocks in a dessicator. I have used this method under low vacuum for GMA blocks. Good Luck. Christie From Heather.A.Harper <@t> pcola.med.navy.mil Fri Aug 11 05:41:38 2006 From: Heather.A.Harper <@t> pcola.med.navy.mil (Harper, Heather A., CIV) Date: Fri Aug 11 09:53:17 2006 Subject: [Histonet] Chatter Message-ID: I have worked with 8 pathologists and have never encountered this problem. This particular pathologist, says that when I cut, my slides have chatter, especially the small biopsies but particularly the intestinal biopsies. My co-worker does the majority of the cutting, yet has never received a comment about "chatter" on her cutting of slides from this pathologist. Could somebody tell me what causes this chatter, especially with intestinal biopsies? Is it the microtome, which I have a new knife holder, so I am good to go there, because it cuts beautifully now. I have no problems getting smooth paraffin sections. This pathologist is making me paranoid, because I have never had this problem with any other pathologists. Thanks and Happy Friday:-) Heather a. Harper Supervisor of Histology Naval Hospital Pensacola, FL From Brandiwork <@t> mchsi.com Fri Aug 11 09:53:31 2006 From: Brandiwork <@t> mchsi.com (Brandi Farris) Date: Fri Aug 11 09:53:46 2006 Subject: [Histonet] A different way to cut?? Message-ID: <000601c6bd55$ed127f00$f2e8d70c@ernie> Hello, I have a new co-worker that has a different way of cutting, he professes that it is the new and improved way but we're unsure and wondering if anyone else has experience on this way. He faces into the block, places the block (not section) into a 42 degree waterbath for a few minutes, then into crushing ice for a few minutes, sprays the block with Frostbite and then obtains his section. Are we just too old school? This seems like a lot of work and hassle. Thank you, Brandi Farris From Barry.R.Rittman <@t> uth.tmc.edu Fri Aug 11 09:58:02 2006 From: Barry.R.Rittman <@t> uth.tmc.edu (Rittman, Barry R) Date: Fri Aug 11 09:58:06 2006 Subject: [Histonet] A different way to cut?? Message-ID: Brandi Is there any chance that you could direct him to another career? Barry -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Brandi Farris Sent: Friday, August 11, 2006 9:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] A different way to cut?? Hello, I have a new co-worker that has a different way of cutting, he professes that it is the new and improved way but we're unsure and wondering if anyone else has experience on this way. He faces into the block, places the block (not section) into a 42 degree waterbath for a few minutes, then into crushing ice for a few minutes, sprays the block with Frostbite and then obtains his section. Are we just too old school? This seems like a lot of work and hassle. Thank you, Brandi Farris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kari.Zajic <@t> HCAhealthcare.com Fri Aug 11 10:00:20 2006 From: Kari.Zajic <@t> HCAhealthcare.com (Zajic Kari) Date: Fri Aug 11 10:00:31 2006 Subject: [Histonet] A different way to cut?? In-Reply-To: <000601c6bd55$ed127f00$f2e8d70c@ernie> Message-ID: <095327C7CDBDF64B9E9728A54799091E015CC5CA@ORLEV03.hca.corpad.net> That not only seems like an unnecessary amount of extra work to me, but isn't it just the same result?? Do his sections "look" better?? To each his own I say...I think everyone varies slightly on how they cut...I've never heard that one though! :) Kari Marie Zajic HT,MLT Histology Supervisor Palms West Hospital Pathology Department 13001 State Road Eighty Loxahatchee, Florida 33470 phone: (561)798-6036 telefax: (561)753-4298 voicemail: (561)753-4299 pager: (561)610-4949 email: Kari.Zajic@HCAHealthcare.com This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Brandi Farris Sent: Friday, August 11, 2006 10:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] A different way to cut?? Hello, I have a new co-worker that has a different way of cutting, he professes that it is the new and improved way but we're unsure and wondering if anyone else has experience on this way. He faces into the block, places the block (not section) into a 42 degree waterbath for a few minutes, then into crushing ice for a few minutes, sprays the block with Frostbite and then obtains his section. Are we just too old school? This seems like a lot of work and hassle. Thank you, Brandi Farris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From PMonfils <@t> Lifespan.org Fri Aug 11 10:15:19 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Aug 11 10:15:25 2006 Subject: [Histonet] A different way to cut?? Message-ID: <09C945920A6B654199F7A58A1D7D1FDE0171777D@lsexch.lsmaster.lifespan.org> This is a good method for addressing problems with certain types of difficult tissues, such as hard, overly dry, bloody or necrotic tissues. But as a routine method for all tissues it really sounds like a waste of valuable time. From limla <@t> mail.nih.gov Fri Aug 11 10:16:46 2006 From: limla <@t> mail.nih.gov (Lim, Langston (NIH/NCI) [E]) Date: Fri Aug 11 10:16:51 2006 Subject: [Histonet] A different way to cut?? In-Reply-To: <095327C7CDBDF64B9E9728A54799091E015CC5CA@ORLEV03.hca.corpad.net> Message-ID: I use to do it to dry, brittle, bloody blocks instead of using ammonium water. It's a little faster but I would not recommend soaking it in the warm water more than 15-30 sec. Langston Lim, HT (ASCP) Tissue Array Research Program DHHS/NIH/NCI/CCR/LP 301-402-4927 -----Original Message----- From: Zajic Kari [mailto:Kari.Zajic@HCAhealthcare.com] Sent: Friday, August 11, 2006 11:00 AM To: Brandi Farris Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] A different way to cut?? That not only seems like an unnecessary amount of extra work to me, but isn't it just the same result?? Do his sections "look" better?? To each his own I say...I think everyone varies slightly on how they cut...I've never heard that one though! :) Kari Marie Zajic HT,MLT Histology Supervisor Palms West Hospital Pathology Department 13001 State Road Eighty Loxahatchee, Florida 33470 phone: (561)798-6036 telefax: (561)753-4298 voicemail: (561)753-4299 pager: (561)610-4949 email: Kari.Zajic@HCAHealthcare.com This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Brandi Farris Sent: Friday, August 11, 2006 10:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] A different way to cut?? Hello, I have a new co-worker that has a different way of cutting, he professes that it is the new and improved way but we're unsure and wondering if anyone else has experience on this way. He faces into the block, places the block (not section) into a 42 degree waterbath for a few minutes, then into crushing ice for a few minutes, sprays the block with Frostbite and then obtains his section. Are we just too old school? This seems like a lot of work and hassle. Thank you, Brandi Farris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Aug 11 10:26:29 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 11 10:26:33 2006 Subject: [Histonet] A different way to cut?? In-Reply-To: <000601c6bd55$ed127f00$f2e8d70c@ernie> Message-ID: <20060811152629.62747.qmail@web61220.mail.yahoo.com> Brandi: After 50 years in this trade I have seen that procedure several times, but not as a routine way of doing things; just for really brittle/difficult blocks. Also the time in the water bath just a few seconds, just to heat the paraffin and produce a greater constriction when the ice is applied afterwards. Ren? J. Brandi Farris wrote: Hello, I have a new co-worker that has a different way of cutting, he professes that it is the new and improved way but we're unsure and wondering if anyone else has experience on this way. He faces into the block, places the block (not section) into a 42 degree waterbath for a few minutes, then into crushing ice for a few minutes, sprays the block with Frostbite and then obtains his section. Are we just too old school? This seems like a lot of work and hassle. Thank you, Brandi Farris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your email and more, right on the new Yahoo.com From vazquezr <@t> ohsu.edu Fri Aug 11 10:26:47 2006 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Fri Aug 11 10:27:15 2006 Subject: [Histonet] Chatter Message-ID: Heather, With intestinal bxs I have found soaking in between each level on your ice block. let it get cold and hydrated. Robyn OHSU From Heather.A.Harper <@t> pcola.med.navy.mil Fri Aug 11 10:30:10 2006 From: Heather.A.Harper <@t> pcola.med.navy.mil (Harper, Heather A., CIV) Date: Fri Aug 11 10:32:01 2006 Subject: [Histonet] Chatter Message-ID: I use Accu Edge, disposable low profile blades. I face my blocks and let them sit on ice, and add some water on top of the ice. I cut between 3-5 degrees. I do everything the normal histo tech does. The blade holder is new and adjusted b/c I went through problems cutting, b/c there was something wrong with the old blade holder. I had the rep do an entire PM on my microtome, which is only a year old. Everything has been adjusted, including block holder. I just find it weird that since this new path came on board, all of a sudden my small biopsies, especially intestinal biopsies, have chatter, but yet my co-worker doesn't get told about her slides having chatter. I'm just trying to figure out if it's me, or it's the path trying to nit pick. If it truly is a problem with my microtome, than I need to get the rep to fix the problem. I asked the other path and he claimed he doesn't see chatter on his slides when I have cut. In reference to the "A different Way to cut", I have never experienced a histo tech who soaks the block in a 42 degree bath, than puts it on crushed ice, and than sprays FrostBite. That is way too many steps, but to each his own. This person might just be paranoid b/c others have questioned his/her technique, so has come up w/the line of it's the new and improved way. As long as they get a good section, let that person be. Heather A. Harper Naval Hospital Pensacola, FL From funderwood <@t> mcohio.org Fri Aug 11 10:32:44 2006 From: funderwood <@t> mcohio.org (Fred Underwood) Date: Fri Aug 11 10:33:30 2006 Subject: [Histonet] Chatter Message-ID: Sounds like you need to eliminate chatter from him. A couple of thoughts. Don't crank the wheel too fast when sectioning, use a slower steady pace. It is possible to over soak small biopsies, making the tissue too soft for good sectioning. You're not alone in dealing with a difficult pathologist. Be strong Heather, you can defeat this evil villian:) Fred >>> "Harper, Heather A., CIV" 08/11 6:41 AM >>> I have worked with 8 pathologists and have never encountered this problem. This particular pathologist, says that when I cut, my slides have chatter, especially the small biopsies but particularly the intestinal biopsies. My co-worker does the majority of the cutting, yet has never received a comment about "chatter" on her cutting of slides from this pathologist. Could somebody tell me what causes this chatter, especially with intestinal biopsies? Is it the microtome, which I have a new knife holder, so I am good to go there, because it cuts beautifully now. I have no problems getting smooth paraffin sections. This pathologist is making me paranoid, because I have never had this problem with any other pathologists. Thanks and Happy Friday:-) Heather a. Harper Supervisor of Histology Naval Hospital Pensacola, FL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Heather.A.Harper <@t> pcola.med.navy.mil Fri Aug 11 10:35:57 2006 From: Heather.A.Harper <@t> pcola.med.navy.mil (Harper, Heather A., CIV) Date: Fri Aug 11 10:37:46 2006 Subject: [Histonet] Chatter Message-ID: Somebody suggested soaking the block on ice between levels. We only do 1 level on intestinal biopsies. So that doesn't help me but if ever I have to do levels, than I will remember that tidbit of advice. Heather A. Harper Naval hospital Pensacola, FL. From rjbuesa <@t> yahoo.com Fri Aug 11 10:48:21 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 11 10:48:24 2006 Subject: [Histonet] Chatter In-Reply-To: Message-ID: <20060811154821.32015.qmail@web61215.mail.yahoo.com> Heather: First things first: is it true that your sections are chattered? How different they are from the other co-worker's sections? I think that you should get slides made by you and by your coworker and take them to the pathologist so he can show you where your chatters are. If you want to modify something you first have to know what to modify. Chattaring has many causes and since it seems that yours are the only ones with chatter, we can eliminate the main cause that is overdrying of the small biopsies while being processed. Really I think that this pathologist should indicate to you the specific areas he considers as being chattered. Remember that communication is the main avenue to tackle all problems between people. After that you can begin fiddling with ice, blades, angles and the like. Hope this will hekp you! Ren? J. "Harper, Heather A., CIV" wrote: I have worked with 8 pathologists and have never encountered this problem. This particular pathologist, says that when I cut, my slides have chatter, especially the small biopsies but particularly the intestinal biopsies. My co-worker does the majority of the cutting, yet has never received a comment about "chatter" on her cutting of slides from this pathologist. Could somebody tell me what causes this chatter, especially with intestinal biopsies? Is it the microtome, which I have a new knife holder, so I am good to go there, because it cuts beautifully now. I have no problems getting smooth paraffin sections. This pathologist is making me paranoid, because I have never had this problem with any other pathologists. Thanks and Happy Friday:-) Heather a. Harper Supervisor of Histology Naval Hospital Pensacola, FL _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail Beta. From Heather.A.Harper <@t> pcola.med.navy.mil Fri Aug 11 10:48:00 2006 From: Heather.A.Harper <@t> pcola.med.navy.mil (Harper, Heather A., CIV) Date: Fri Aug 11 10:49:49 2006 Subject: [Histonet] Chatter Message-ID: I have gotten some good responses about the chatter problem. I'm not sure about the cutting fast, wheel rotating fast. We do one level, and I'm a steady cutter. I care about what my sections look like. So I can't say I'm one rotating the wheel fast. I'll figure this all out sooner or later. I'll experiment and do the process of elimination. Thank you everybody for you input. Heather A. Harper Naval Hospital Pensacola, FL From oshel1pe <@t> cmich.edu Fri Aug 11 11:01:47 2006 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Fri Aug 11 11:01:57 2006 Subject: [Histonet] Chatter In-Reply-To: <20060811154821.32015.qmail@web61215.mail.yahoo.com> References: <20060811154821.32015.qmail@web61215.mail.yahoo.com> Message-ID: One quick test would be to do a blind test: *Use a test specimen not a real clinical specimen.* You cut slides and your coworker cut slides from the same block. You label her slide as per usual and turn it in as your sectioning, and your coworker labels your slide as per usual as her sectioning. Turn in both to the pathologist in question and see what he says. If you still have chatter and she doesn't, then you know there is nothing wrong with your slides. Phil >"Harper, Heather A., CIV" wrote: > I have worked with 8 pathologists and have never encountered this problem. >This particular pathologist, says that when I cut, my slides have chatter, >especially the small biopsies but particularly the intestinal biopsies. My >co-worker does the majority of the cutting, yet has never received a comment >about "chatter" on her cutting of slides from this pathologist. Could >somebody tell me what causes this chatter, especially with intestinal >biopsies? Is it the microtome, which I have a new knife holder, so I am good >to go there, because it cuts beautifully now. I have no problems getting >smooth paraffin sections. This pathologist is making me paranoid, because I >have never had this problem with any other pathologists. Thanks and Happy >Friday:-) > > > >Heather a. Harper > >Supervisor of Histology > >Naval Hospital > >Pensacola, FL -- Philip Oshel Microscopy Facility Supervisor Department of Biology Central Michigan University 024C Brooks Hall Mt. Pleasant, MI 48859 voice: (989) 774-3576 dept. fax: (989) 774-3462 From lrichey <@t> u.washington.edu Fri Aug 11 12:24:20 2006 From: lrichey <@t> u.washington.edu (Lori Richey) Date: Fri Aug 11 12:24:32 2006 Subject: [Histonet] A different way to cut?? In-Reply-To: <000601c6bd55$ed127f00$f2e8d70c@ernie> References: <000601c6bd55$ed127f00$f2e8d70c@ernie> Message-ID: <44DCBD44.8000101@u.washington.edu> I've heard of that technique when trying to cut dried out and bloody specimens or dry lymph nodes. Not necessarily a 42 degree temp, but warming the block in the water bath first, and then back on the ice. The "freeze it" is optional and not necessary. Brandi Farris wrote: >Hello, > >I have a new co-worker that has a different way of cutting, he professes that it is the new and improved way but we're unsure and wondering if anyone else has experience on this way. He faces into the block, places the block (not section) into a 42 degree waterbath for a few minutes, then into crushing ice for a few minutes, sprays the block with Frostbite and then obtains his section. Are we just too old school? This seems like a lot of work and hassle. > >Thank you, >Brandi Farris >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From godsgirlnow <@t> msn.com Fri Aug 11 12:31:44 2006 From: godsgirlnow <@t> msn.com (Roxanne Soto) Date: Fri Aug 11 12:31:53 2006 Subject: [Histonet] Chatter In-Reply-To: Message-ID: Have you looked at your sections? ______________________________________________________________ From: "Harper, Heather A., CIV" To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Chatter Date: Fri, 11 Aug 2006 10:48:00 -0500 > I have gotten some good responses about the chatter problem. I'm not sure >about the cutting fast, wheel rotating fast. We do one level, and I'm a >steady cutter. I care about what my sections look like. So I can't say I'm >one rotating the wheel fast. I'll figure this all out sooner or later. I'll >experiment and do the process of elimination. Thank you everybody for you >input. > > > >Heather A. Harper > >Naval Hospital > >Pensacola, FL > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Julie.Sanders <@t> va.gov Fri Aug 11 12:32:05 2006 From: Julie.Sanders <@t> va.gov (Sanders, Julie, VHACIN) Date: Fri Aug 11 12:32:13 2006 Subject: [Histonet] RE: Histonet Digest, Vol 33, Issue 14 In-Reply-To: <4vij0p$1huk78@mtares1.res.net.va.gov> Message-ID: <2D4ACE41DEFE93428F23D77988EFBCB50E606C@VHAV10MSGA2.v10.med.va.gov> I've heard of putting it in the water bath, and then ice, but my opinion is that spraying it with any freezing spray cause artifact. When I first came here that's what the techs did (use cyrospray) and I discouraged it and all of a sudden the pathologists were discussing how much better the slides looked. We normally soak all of our GI biopsies in ice water before cutting. Julie Sanders Hello, I have a new co-worker that has a different way of cutting, he professes that it is the new and improved way but we're unsure and wondering if anyone else has experience on this way. He faces into the block, places the block (not section) into a 42 degree waterbath for a few minutes, then into crushing ice for a few minutes, sprays the block with Frostbite and then obtains his section. Are we just too old school? This seems like a lot of work and hassle. Thank you, Brandi Farris From dmccaig <@t> ckha.on.ca Fri Aug 11 12:34:52 2006 From: dmccaig <@t> ckha.on.ca (Diana McCaig) Date: Fri Aug 11 12:40:00 2006 Subject: [Histonet] recycling alcohol,xylene and formalin Message-ID: Any opinions or advise regarding recyclers. Any input would be appreciated. Diana McCaig 519-352-6401 (6604) From Barbara_Lentz <@t> dahlchase.com Fri Aug 11 13:03:18 2006 From: Barbara_Lentz <@t> dahlchase.com (Barbara Lentz) Date: Fri Aug 11 13:03:34 2006 Subject: [Histonet] A different way to cut?? Message-ID: Hi Brandi, I do something similar to his procedure for bloody blocks: I float the block on my waterbath for 10 - 15 seconds, then place on ice. I do this back and forth a couple of times. I find it totally unnecessary to use anything like "Frostbite." The sections cut from the block are much better--the blood doesn't fragment. Barb >>> "Brandi Farris" 08/11/06 10:53AM >>> Hello, I have a new co-worker that has a different way of cutting, he professes that it is the new and improved way but we're unsure and wondering if anyone else has experience on this way. He faces into the block, places the block (not section) into a 42 degree waterbath for a few minutes, then into crushing ice for a few minutes, sprays the block with Frostbite and then obtains his section. Are we just too old school? This seems like a lot of work and hassle. Thank you, Brandi Farris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From fran_lemons <@t> yahoo.com Fri Aug 11 13:16:19 2006 From: fran_lemons <@t> yahoo.com (Fran Walker) Date: Fri Aug 11 13:16:22 2006 Subject: [Histonet] Somehing in writing Message-ID: <20060811181619.4658.qmail@web61316.mail.yahoo.com> Can anybody refer me to anything in writing that says a tech must be HTL vs. HT in order to perform IHC? Not looking for recommendations or opinions, albeit appreciated, but need to see this in writing by a governing authority. CAP, CLIA, JCAHO, whoever. Local policy doesn't count. Thanks Fran Walker, HT (ASCP) --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail Beta. From Kari.Zajic <@t> HCAhealthcare.com Fri Aug 11 13:31:05 2006 From: Kari.Zajic <@t> HCAhealthcare.com (Zajic Kari) Date: Fri Aug 11 13:31:08 2006 Subject: [Histonet] Somehing in writing In-Reply-To: <20060811181619.4658.qmail@web61316.mail.yahoo.com> Message-ID: <095327C7CDBDF64B9E9728A54799091E015CC5D1@ORLEV03.hca.corpad.net> I think it states it in the CAP checklist (you can download it on the CAP website) but not completely sure... Kari Marie Zajic HT,MLT Histology Supervisor Palms West Hospital Pathology Department 13001 State Road Eighty Loxahatchee, Florida 33470 phone: (561)798-6036 telefax: (561)753-4298 voicemail: (561)753-4299 pager: (561)610-4949 email: Kari.Zajic@HCAHealthcare.com This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Fran Walker Sent: Friday, August 11, 2006 2:16 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Somehing in writing Can anybody refer me to anything in writing that says a tech must be HTL vs. HT in order to perform IHC? Not looking for recommendations or opinions, albeit appreciated, but need to see this in writing by a governing authority. CAP, CLIA, JCAHO, whoever. Local policy doesn't count. Thanks Fran Walker, HT (ASCP) --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail Beta. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sjchtascp <@t> yahoo.com Fri Aug 11 14:43:35 2006 From: sjchtascp <@t> yahoo.com (Steven Coakley) Date: Fri Aug 11 14:43:38 2006 Subject: [Histonet] liver albumin Message-ID: <20060811194335.60546.qmail@web38213.mail.mud.yahoo.com> Good afternoon, We are attempting to stain cyrosections of mouse liver for albumin. We have currently tried both unfixed and fixed/sucrose sectioned postfixed in 4% Formalin/PBS. Vascular stains but otherwise nothing. Is anyone doing anything like this? Thanks, Steve --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. From mcauliff <@t> umdnj.edu Fri Aug 11 15:05:57 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Aug 11 15:05:26 2006 Subject: [Histonet] liver albumin In-Reply-To: <20060811194335.60546.qmail@web38213.mail.mud.yahoo.com> References: <20060811194335.60546.qmail@web38213.mail.mud.yahoo.com> Message-ID: <44DCE325.2080909@umdnj.edu> Hi Steve: Albumin is a very large protein that is largely confined to the vascular system. Yes, it is made in hepatocytes but I suspect that it diffuses quickly in sections. Also, the form that exists inside cells may not react with your marker? I suspect that freeze drying or freeze substitution might (emphasis on might) be the way to go here. Also, a PubMed or Medline search will reach a much larger database than Histonet and you might find the methods you seek. Geoff Steven Coakley wrote: >Good afternoon, > We are attempting to stain cyrosections of mouse liver for albumin. We have currently tried both unfixed and fixed/sucrose sectioned postfixed in 4% Formalin/PBS. Vascular stains but otherwise nothing. Is anyone doing anything like this? > > Thanks, > > Steve > > >--------------------------------- >Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From schloesr <@t> mail.nih.gov Fri Aug 11 15:19:19 2006 From: schloesr <@t> mail.nih.gov (Robert J. Schloesser) Date: Fri Aug 11 15:17:28 2006 Subject: [Histonet] brain inflammation Message-ID: <000001c6bd83$6d100650$76e4bb89@yourd54swmkxh6> Hi, Which antibodies/stains would you recommend to evaluate/quantify inflammation in fixed rat or mouse tissue? Thank you Robert From liz <@t> premierlab.com Fri Aug 11 15:32:37 2006 From: liz <@t> premierlab.com (Liz Chlipala) Date: Fri Aug 11 15:27:37 2006 Subject: [Histonet] brain inflammation In-Reply-To: <000001c6bd83$6d100650$76e4bb89@yourd54swmkxh6> Message-ID: <000001c6bd85$48b63ac0$0300a8c0@domain.Premier> Robert Couldn't you just look at an H&E and score for general inflammation. If you wanted to get more detailed then you could look at different markers for specific inflammatory cells. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Robert J. Schloesser Sent: Friday, August 11, 2006 2:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] brain inflammation Hi, Which antibodies/stains would you recommend to evaluate/quantify inflammation in fixed rat or mouse tissue? Thank you Robert _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1.1703 (20060811) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From mcauliff <@t> umdnj.edu Fri Aug 11 15:45:42 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Fri Aug 11 15:45:12 2006 Subject: [Histonet] How to Embed in High-humidity env. (???) In-Reply-To: References: Message-ID: <44DCEC76.50508@umdnj.edu> Hi Larry: Make sure you are using the correct formulation for Spurr's resin. If you are using the new formulation with ERL 4221 instead of 4206 the recipe provided by your vendor is almost certainly wrong and will lead to exactly the kind of problems you are having. I know, I have been there. Check the most recent issue of Microsocpy Today for the correct formulation. E. Ann Ellis deserves the credit for working this out. Geoff Lawrence D Lanberg/O/VCU wrote: >Dear Histonetters, > >I embed mouse eyeballs in Spurr's Formula (epoxide), after dehydrating them >in a series of increasing EtOh strengths. I section these with a glass >knife, 0.99 micron thick. I am hoping someone very familiar with Spurr's >can help. > >Beginning very recently, the blocks are poorly cured in the central cavity >of the eye (outside is ok). This problem began with the onset of summer; >prolonged baking does not help. I've even tried a 'rapid cure' modification >-- more than doubling the DMAE accelerator. Our best guess is that >environmental moisture is the culprit. > >I understand that epoxides do not work well in humidity. Unforutnately, I'm >in Richmond Virginia, where one can literally poach an egg by holding it up >in the air. The humidity goes right through air-conditioned walls. > >Does anyone know of some modifications I can make, to where my Spurrr / >epoxide embedding will be successful in the high-humidity environment? I >would be very grateful. > >Larry Lanberg > > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From lanbergld <@t> vcu.edu Fri Aug 11 16:56:15 2006 From: lanbergld <@t> vcu.edu (Lawrence D Lanberg/O/VCU) Date: Fri Aug 11 16:56:23 2006 Subject: [Histonet] Responses to my Embedding question (humidity) Message-ID: I am very grateful for the replies I have received, concerning my embedding issue. I was busy in the lab so it was almost impossible to send individual thanks to each member. I have made a list of all the tips I got; will try each one in good order. In addition, I will be inserting 3A molecular sieves into my ethanol bottle(s), hoping to dry this back out. I mean it is really humid here! Its great there is this network where those with expertise willingly share, asking for nothing in return. Other disciplines should be so lucky. Sincerely, Larry Lanberg PO Box 980614 Sanger Hall 2-032 Richmond, VA 23298 804.828.7974 804.648.3246 From jnocito <@t> satx.rr.com Sat Aug 12 05:35:02 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Sat Aug 12 05:35:09 2006 Subject: [Histonet] Chatter References: Message-ID: <008401c6bdfa$f809bfe0$4a624542@yourxhtr8hvc4p> I had a pathologist like that. They can't find him now. I shot him. Seriously, we had the same issues. We put a little 1% ammonium hydroxide on top of the ice tray with some water. Not too much, don't want to be overcome by fumes. This seemed to work. We do levels on our GI bxs and I can tell we people don't soak the block enough, the third level has chatter. So I shoot them. I have a couple of openings now. Anyone want to apply? Gee, I love Fridays!!!! Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Harper, Heather A., CIV" To: Sent: Friday, August 11, 2006 5:41 AM Subject: [Histonet] Chatter > I have worked with 8 pathologists and have never encountered this > problem. > This particular pathologist, says that when I cut, my slides have chatter, > especially the small biopsies but particularly the intestinal biopsies. My > co-worker does the majority of the cutting, yet has never received a > comment > about "chatter" on her cutting of slides from this pathologist. Could > somebody tell me what causes this chatter, especially with intestinal > biopsies? Is it the microtome, which I have a new knife holder, so I am > good > to go there, because it cuts beautifully now. I have no problems getting > smooth paraffin sections. This pathologist is making me paranoid, because > I > have never had this problem with any other pathologists. Thanks and Happy > Friday:-) > > > > Heather a. Harper > > Supervisor of Histology > > Naval Hospital > > Pensacola, FL > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.405 / Virus Database: 268.10.9/416 - Release Date: 8/10/2006 > From GAshton <@t> PICR.man.ac.uk Sat Aug 12 07:38:58 2006 From: GAshton <@t> PICR.man.ac.uk (Garry Ashton) Date: Sat Aug 12 08:48:43 2006 Subject: [Histonet] autostainers & TMA's Message-ID: Dear histonetters I have 2 topics up for discussion. Firstly immunostainers. We are a small research lab and hence I'm interested in a machine that is totally flexible with regards to running several antibodies with 2 /3 different methods. Ideally an "open system" that will also dewax & retrieve if possible. We've looked at dako and biogenix which are both open systems and ventana and vision biosytems. Any comments on the above, or on any other systems available would be great. I know this subject ha been covered several times on the histonet, but an up to date view would be much appreciated. Secondly has anybody had any problems with regards to TMA sections falling off slides. We use 1mm cores and only put about 100 in each block, which are then cut and placed on APES coated slides. Sometimes up to 30% of the core may come off. I think next we'll try charged slides. Does anybody have any opinions / ideas on this Many thanks in advance for any opinions. Garry Ashton PICR UK -------------------------------------------------------- This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the University of Manchester. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. From redaktion <@t> medizin.at Sat Aug 12 14:59:37 2006 From: redaktion <@t> medizin.at (Team medizin.at) Date: Sat Aug 12 14:59:34 2006 Subject: [Histonet] FW: sporadic CJD Message-ID: Hi, i?ve been asked to work up some formalin fixed heart, lung, liver etc. but NOT brain or CNS from a case with sporadic CJD. I?ve been told that this can be done without any precautions, like every other specimen. Is this true? And if not true, how should the specimens be treated? Please help and many thanks in advance! Cristin From pruegg <@t> ihctech.net Sat Aug 12 16:56:30 2006 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Sat Aug 12 16:56:45 2006 Subject: [Histonet] Setting Ink In-Reply-To: Message-ID: <200608122156.k7CLuXf1045204@pro12.abac.com> We use Bouins fixative to set ink, just dip it in for a few seconds then go on your way. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Wednesday, August 09, 2006 8:04 AM To: Histonet (E-mail) Subject: [Histonet] Setting Ink Cheers Everyone, Does anyone know if there is something that sets the ink on tissue after you have inked the margins. I vaguely remember something but I cannot seemed to dig it out of my brain. Thanks in advance, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathololgy Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu From sheila_adey <@t> hotmail.com Sun Aug 13 12:16:20 2006 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Sun Aug 13 12:16:28 2006 Subject: [Histonet] autostainers & TMA's In-Reply-To: Message-ID: Hi Garry, We currently have a demo of the Biogenex i6000. We love it. You can modify each individual slide, instead of editing a whole new procedure. We mostly wanted it for the random access. We already have the Artisan by Dako. A reliable instrument but with limited kit availability. Sheila Adey HT, MLT Port Huron Hospital Michigan >From: "Garry Ashton" >To: >Subject: [Histonet] autostainers & TMA's >Date: Sat, 12 Aug 2006 13:38:58 +0100 > >Dear histonetters >I have 2 topics up for discussion. > >Firstly immunostainers. >We are a small research lab and hence I'm interested in a machine that >is totally flexible with regards to running several antibodies with 2 /3 >different methods. >Ideally an "open system" that will also dewax & retrieve if possible. >We've looked at dako and biogenix which are both open systems and >ventana and vision biosytems. >Any comments on the above, or on any other systems available would be >great. >I know this subject ha been covered several times on the histonet, but >an up to date view would be much appreciated. > >Secondly has anybody had any problems with regards to TMA sections >falling off slides. >We use 1mm cores and only put about 100 in each block, which are then >cut and placed on APES coated slides. >Sometimes up to 30% of the core may come off. >I think next we'll try charged slides. Does anybody have any opinions / >ideas on this > >Many thanks in advance for any opinions. > > > > > >Garry Ashton >PICR >UK > >-------------------------------------------------------- > > >This email is confidential and intended solely for the use of the person(s) >('the intended recipient') to whom it was addressed. Any views or opinions >presented are solely those of the author and do not necessarily represent >those of the Paterson Institute for Cancer Research or the University of >Manchester. It may contain information that is privileged & confidential >within the meaning of applicable law. Accordingly any dissemination, >distribution, copying, or other use of this message, or any of its >contents, by any person other than the intended recipient may constitute a >breach of civil or criminal law and is strictly prohibited. If you are NOT >the intended recipient please contact the sender and dispose of this e-mail >as soon as possible. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Play Q6 for your chance to WIN great prizes. http://q6trivia.imagine-live.com/enca/landing From pruegg <@t> ihctech.net Sun Aug 13 13:06:06 2006 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Sun Aug 13 13:06:17 2006 Subject: [SPAM] RE: [Histonet] autostainers & TMA's In-Reply-To: Message-ID: <200608131806.k7DI69ht007397@pro12.abac.com> The DAKO autostainer is totally open, can run several different antibodies with different detections and individual slide modifications. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sheila adey Sent: Sunday, August 13, 2006 11:16 AM To: GAshton@PICR.man.ac.uk; histonet@lists.utsouthwestern.edu Subject: [SPAM] RE: [Histonet] autostainers & TMA's Hi Garry, We currently have a demo of the Biogenex i6000. We love it. You can modify each individual slide, instead of editing a whole new procedure. We mostly wanted it for the random access. We already have the Artisan by Dako. A reliable instrument but with limited kit availability. Sheila Adey HT, MLT Port Huron Hospital Michigan >From: "Garry Ashton" >To: >Subject: [Histonet] autostainers & TMA's >Date: Sat, 12 Aug 2006 13:38:58 +0100 > >Dear histonetters >I have 2 topics up for discussion. > >Firstly immunostainers. >We are a small research lab and hence I'm interested in a machine that >is totally flexible with regards to running several antibodies with 2 /3 >different methods. >Ideally an "open system" that will also dewax & retrieve if possible. >We've looked at dako and biogenix which are both open systems and >ventana and vision biosytems. >Any comments on the above, or on any other systems available would be >great. >I know this subject ha been covered several times on the histonet, but >an up to date view would be much appreciated. > >Secondly has anybody had any problems with regards to TMA sections >falling off slides. >We use 1mm cores and only put about 100 in each block, which are then >cut and placed on APES coated slides. >Sometimes up to 30% of the core may come off. >I think next we'll try charged slides. Does anybody have any opinions / >ideas on this > >Many thanks in advance for any opinions. > > > > > >Garry Ashton >PICR >UK > >-------------------------------------------------------- > > >This email is confidential and intended solely for the use of the person(s) >('the intended recipient') to whom it was addressed. Any views or opinions >presented are solely those of the author and do not necessarily represent >those of the Paterson Institute for Cancer Research or the University of >Manchester. It may contain information that is privileged & confidential >within the meaning of applicable law. Accordingly any dissemination, >distribution, copying, or other use of this message, or any of its >contents, by any person other than the intended recipient may constitute a >breach of civil or criminal law and is strictly prohibited. If you are NOT >the intended recipient please contact the sender and dispose of this e-mail >as soon as possible. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Play Q6 for your chance to WIN great prizes. http://q6trivia.imagine-live.com/enca/landing _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Sun Aug 13 13:08:20 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Sun Aug 13 13:08:29 2006 Subject: [Histonet] Re: Setting Ink Message-ID: <35f.a625034.3210c494@aol.com> Karen Heckford asks >>Does anyone know if there is something that sets the ink on tissue after you have inked the margins?<< and Patsy Ruegg (along with several others) replies >>We use Bouin's fixative to set ink, just dip it in for a few seconds then go on your way.<< Bouin's fixative contains picric acid, a potential explosion hazard that can give you trouble with regulatory agencies, besides being toxic, and ruinous to clothing (particularly since it is inclined to eat through latex). Acetone, also often recommended, is a fire and explosion hazard. If 2% to 3% acetic acid works - and I haven't tried it personally - why not use that? If you blot the specimen dry adequately, the ink will usually stick to it - India ink, Davidson and similar marking inks, and tattoo inks - I've used them all. Ink won't stick to cauterized surfaces (such as LEEP specimens), but the pathologist can see these margins under the microscope whether they're inked or not. Bob Richmond From RSRICHMOND <@t> aol.com Sun Aug 13 13:17:11 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Sun Aug 13 13:17:23 2006 Subject: [Histonet] Re: sporadic CJD Message-ID: Cristin irgendwo in Oesterreich fragt: >>I've been asked to work up some formalin fixed heart, lung, liver etc. but NOT brain or CNS from a case with sporadic CJD. I've been told that this can be done without any precautions, like every other specimen.<< All tissues from a patient with Creutzfeldt-Jakob disease (CJD) should be regarded as potentially infectious. Consult the CDC Web site at http://www.cdc.gov/ncidod/dvrd/cjd/infection_control_cjd.htm and obtain some expert advice with this problem. Allein unter den Deutschspraechigen der Welt koennen die Oesterreicher lachen - bitte aber nicht mit Lachenskrankheit (Kuru). Bob Richmond Knoxville, Tennessee and Gastonia, North Caroliina From marjoh3 <@t> telus.net Sun Aug 13 13:56:09 2006 From: marjoh3 <@t> telus.net (Marilyn Johnson) Date: Sun Aug 13 13:56:15 2006 Subject: [Histonet] Demonstration of Blue Tongue by Immunohistochemistry Message-ID: <000a01c6bf0a$23edc100$6401a8c0@VALUED20606295> Hi Veterinary Histonetters, Do any Veterinary Diagnostic Labs. test for Blue Tongue by IHC? Please let me know. Any replies would be greatly appreciated. Thank you in advance. Marilyn Johnson Alberta Agriculture, Edmonton, Alberta, Canada. From gu.lang <@t> gmx.at Sun Aug 13 14:38:47 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Sun Aug 13 14:38:50 2006 Subject: [Histonet] AW: Setting Ink In-Reply-To: <35f.a625034.3210c494@aol.com> Message-ID: <000901c6bf10$1898b200$eeeea8c0@SERVER01> We paint the margins with black indian ink, then dry the surface with an hairdryer until it becomes shiny. We usually do this with mamma tissue, prostatae and skin. There was no negativ effect noticed on the tissue, and the black margins are well seen in the section. Gudrun Lang -----Urspr?ngliche Nachricht----- Von: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von RSRICHMOND@aol.com Gesendet: Sonntag, 13. August 2006 20:08 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: Setting Ink Karen Heckford asks >>Does anyone know if there is something that sets the ink on tissue after you have inked the margins?<< and Patsy Ruegg (along with several others) replies >>We use Bouin's fixative to set ink, just dip it in for a few seconds then go on your way.<< Bouin's fixative contains picric acid, a potential explosion hazard that can give you trouble with regulatory agencies, besides being toxic, and ruinous to clothing (particularly since it is inclined to eat through latex). Acetone, also often recommended, is a fire and explosion hazard. If 2% to 3% acetic acid works - and I haven't tried it personally - why not use that? If you blot the specimen dry adequately, the ink will usually stick to it - India ink, Davidson and similar marking inks, and tattoo inks - I've used them all. Ink won't stick to cauterized surfaces (such as LEEP specimens), but the pathologist can see these margins under the microscope whether they're inked or not. Bob Richmond _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From a.schmidt <@t> fuse.net Sun Aug 13 15:45:27 2006 From: a.schmidt <@t> fuse.net (a.schmidt@fuse.net) Date: Sun Aug 13 15:45:32 2006 Subject: [Histonet] Rapid Tissue Processor Message-ID: <8309429.1155501927975.JavaMail.root@webmail6> To All, We have the new Sakura Rapid Tissue Processor and I could really use some feedback! We seem to be having trouble with inconsistencies in fixation--staining artifact and all sorts of things! We were processing about 75% of our surgical specimens on it and have since cut back to just small biopsies because of pathologist complaints. Help---any suggestions as what worked for you!! Sakura is coming in this week to help us too!! Thanks Angie Schmidt Tri-Health Labs--Cincinnati,Ohio From zumbor <@t> email.cs.nsw.gov.au Sun Aug 13 17:23:16 2006 From: zumbor <@t> email.cs.nsw.gov.au (Rosalba) Date: Sun Aug 13 17:25:14 2006 Subject: [Histonet] A different way to cut?? Message-ID: <01C6BF7A.E5260FB0.zumbor@email.cs.nsw.gov.au> We generally for all dry & brittle blocks especially bloody blocks soak them in very cold water for a few minutes, we have found this to be much better than soaking in warm water as you don't get ice forming on the surface. Rosalba Zumbo Supervisor Histology Dept Department of Forensic Medicine 42-50 Parramatta Rd Glebe NSW 2037 Australia PH: 61 2 85847842 FAX: 61 2 95664573 -----Original Message----- From: Brandi Farris [SMTP:Brandiwork@mchsi.com] Sent: Saturday, 12 August 2006 12:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] A different way to cut?? Hello, I have a new co-worker that has a different way of cutting, he professes that it is the new and improved way but we're unsure and wondering if anyone else has experience on this way. He faces into the block, places the block (not section) into a 42 degree waterbath for a few minutes, then into crushing ice for a few minutes, sprays the block with Frostbite and then obtains his section. Are we just too old school? This seems like a lot of work and hassle. Thank you, Brandi Farris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____________________________________________________________________ This email has been scanned for the Sydney South West Area Health Service by the MessageLabs Email Security System. SSWAHS regularly monitors emails and attachments to ensure compliance with the NSW Government's Electronic Messaging Policy. "This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please destroy it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of the Sydney South West Area Health Service." From pruegg <@t> ihctech.net Sun Aug 13 18:17:32 2006 From: pruegg <@t> ihctech.net (pruegg@ihctech.net) Date: Sun Aug 13 18:17:43 2006 Subject: [Histonet] Re: Setting Ink In-Reply-To: <35f.a625034.3210c494@aol.com> Message-ID: <200608132317.k7DNHZKR002483@pro12.abac.com> I just heard from someone suggesting vinegar (ta da acetic acid), so I think you suggestion would work Bob. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of RSRICHMOND@aol.com Sent: Sunday, August 13, 2006 12:08 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Setting Ink Karen Heckford asks >>Does anyone know if there is something that sets the ink on tissue after you have inked the margins?<< and Patsy Ruegg (along with several others) replies >>We use Bouin's fixative to set ink, just dip it in for a few seconds then go on your way.<< Bouin's fixative contains picric acid, a potential explosion hazard that can give you trouble with regulatory agencies, besides being toxic, and ruinous to clothing (particularly since it is inclined to eat through latex). Acetone, also often recommended, is a fire and explosion hazard. If 2% to 3% acetic acid works - and I haven't tried it personally - why not use that? If you blot the specimen dry adequately, the ink will usually stick to it - India ink, Davidson and similar marking inks, and tattoo inks - I've used them all. Ink won't stick to cauterized surfaces (such as LEEP specimens), but the pathologist can see these margins under the microscope whether they're inked or not. Bob Richmond _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kim.Osullivan <@t> med.monash.edu.au Sun Aug 13 18:41:00 2006 From: Kim.Osullivan <@t> med.monash.edu.au (Kim O'Sullivan) Date: Sun Aug 13 19:11:14 2006 Subject: [Histonet] contaminants in tissue processor Message-ID: <144.138.164.171.1155512179@my.monash.edu.au> Hi everyone, We have a very old citadel tissue processor that we use to process mouse kidney,liver and joints. We have found recently white blooms (best description I can think of- looks like cotton wool) in the first pot of histosol after the last alcohol- does anyone know what these could be and how to prevent them? Secondly since we have changed from using xylene to histosol we seem to be getting a toffee like substance on the bottom of the wax pots-is there any way we can prevent this? Any suggestions would be helpful KimO'Sullivan From rhessler <@t> pathgroup.com Mon Aug 14 06:35:49 2006 From: rhessler <@t> pathgroup.com (Richard Hessler, M.D.) Date: Mon Aug 14 06:36:43 2006 Subject: [Histonet] RE: sporadic CJD Message-ID: There is no reason not to pre-treat for the sake of safety. The following is from the NIH reccomendations: Adequate fixation of small tissue samples (e.g. biopsies) from a patient with suspected prion disease is followed by post-fixation in 96% absolute formic acid for 30 minutes, followed by 48 hours in fresh 10% formalin. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu Sent: Sunday, August 13, 2006 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 33, Issue 16 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. FW: sporadic CJD (Team medizin.at) 2. RE: Setting Ink (pruegg@ihctech.net) ---------------------------------------------------------------------- Message: 1 Date: Sat, 12 Aug 2006 21:59:37 +0200 From: "Team medizin.at" Subject: [Histonet] FW: sporadic CJD To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Hi, i?ve been asked to work up some formalin fixed heart, lung, liver etc. but NOT brain or CNS from a case with sporadic CJD. I?ve been told that this can be done without any precautions, like every other specimen. Is this true? And if not true, how should the specimens be treated? Please help and many thanks in advance! Cristin ------------------------------ Message: 2 Date: Sat, 12 Aug 2006 15:56:30 -0600 From: pruegg@ihctech.net Subject: RE: [Histonet] Setting Ink To: "'Heckford, Karen - SMMC-SF'" , "'Histonet \(E-mail\)'" Message-ID: <200608122156.k7CLuXf1045204@pro12.abac.com> Content-Type: text/plain; charset="us-ascii" We use Bouins fixative to set ink, just dip it in for a few seconds then go on your way. Patsy -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Heckford, Karen - SMMC-SF Sent: Wednesday, August 09, 2006 8:04 AM To: Histonet (E-mail) Subject: [Histonet] Setting Ink Cheers Everyone, Does anyone know if there is something that sets the ink on tissue after you have inked the margins. I vaguely remember something but I cannot seemed to dig it out of my brain. Thanks in advance, Karen Heckford HT (ASCP) CE Lead Histology Technician Histology/Pathololgy Department St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 Fax: 415-750-8123 email: kheckfor@chw.edu ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 33, Issue 16 **************************************** --------------------------------------------------------------------- Important Notice: This e-mail is intended for the use of the person to whom it is addressed and may contain information that is privileged and confidential. If you are not the intended recipient, any disclosure, copying, distribution, or use of the contents of this message is strictly prohibited. If you have received this e-mail in error, please destroy this message and contact the Security Officer at PathGroup, Inc. immediately at 615-562-9255. From hfedor <@t> jhmi.edu Mon Aug 14 07:43:48 2006 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Mon Aug 14 07:44:03 2006 Subject: [Histonet] autostainers & TMA's In-Reply-To: References: Message-ID: <44E037C4.61A1.0088.3@jhmi.edu> Dear Garry, In our TMA Core Facility we use Charged slides, routinely we are using the Capillary Gap slides from Fisher. we dry all TMA slides vertically overnight at room temperature. and they store them at -20. We do have experience tissue loss with this method. Helen Helen L. Fedor B.S. Johns Hopkins University Pathology Department 600 N Wolfe St Marburg Room 406 Baltimore MD 21287 email: hfedor@jhmi.edu Phone: 410 614-1660 Pager: 410 283-3419 WARNING: E-mail sent over the Internet is not secure. Information sent by e-mail may not remain confidential. DISCLAIMER: This e-mail is intended only for the individual to whom it is addressed. It may be used only in accordance with applicable laws. If you received this e-mail by mistake, notify the sender and destroy the e-mail. >>> Garry Ashton 8/12/2006 8:38 AM >>> Dear histonetters I have 2 topics up for discussion. Firstly immunostainers. We are a small research lab and hence I'm interested in a machine that is totally flexible with regards to running several antibodies with 2 /3 different methods. Ideally an "open system" that will also dewax & retrieve if possible. We've looked at dako and biogenix which are both open systems and ventana and vision biosytems. Any comments on the above, or on any other systems available would be great. I know this subject ha been covered several times on the histonet, but an up to date view would be much appreciated. Secondly has anybody had any problems with regards to TMA sections falling off slides. We use 1mm cores and only put about 100 in each block, which are then cut and placed on APES coated slides. Sometimes up to 30% of the core may come off. I think next we'll try charged slides. Does anybody have any opinions / ideas on this Many thanks in advance for any opinions. Garry Ashton PICR UK -------------------------------------------------------- This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the University of Manchester. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From hfedor <@t> jhmi.edu Mon Aug 14 08:53:25 2006 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Mon Aug 14 08:53:45 2006 Subject: [Histonet] autostainers & TMA's In-Reply-To: References: Message-ID: <44E04815.61A1.0088.3@jhmi.edu> Sorry, for the last sentence. It should be: We do not experience tissue loss with this method. Helen >>> Garry Ashton 8/12/2006 8:38 AM >>> Dear histonetters I have 2 topics up for discussion. Firstly immunostainers. We are a small research lab and hence I'm interested in a machine that is totally flexible with regards to running several antibodies with 2 /3 different methods. Ideally an "open system" that will also dewax & retrieve if possible. We've looked at dako and biogenix which are both open systems and ventana and vision biosytems. Any comments on the above, or on any other systems available would be great. I know this subject ha been covered several times on the histonet, but an up to date view would be much appreciated. Secondly has anybody had any problems with regards to TMA sections falling off slides. We use 1mm cores and only put about 100 in each block, which are then cut and placed on APES coated slides. Sometimes up to 30% of the core may come off. I think next we'll try charged slides. Does anybody have any opinions / ideas on this Many thanks in advance for any opinions. Garry Ashton PICR UK -------------------------------------------------------- This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the University of Manchester. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Mon Aug 14 10:17:01 2006 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Aug 14 10:17:09 2006 Subject: [Histonet] autostainers & TMA's In-Reply-To: <44E04815.61A1.0088.3@jhmi.edu> Message-ID: <200608141516.k7EFGuqX001165@chip.viawest.net> Because these sections are so precious, we use the tape transfer system for them, the sections are cut using a tape which is then rolled onto to polymer coated slides, the slides are exposed to UV causing the section to polymerize to the slide allowing the tape to be removed. This is the Instrumedics section aide system which was just bought by McCormick. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Helen Fedor Sent: Monday, August 14, 2006 6:53 AM To: histonet@lists.utsouthwestern.edu; GAshton@PICR.man.ac.uk Subject: Re: [Histonet] autostainers & TMA's Sorry, for the last sentence. It should be: We do not experience tissue loss with this method. Helen >>> Garry Ashton 8/12/2006 8:38 AM >>> Dear histonetters I have 2 topics up for discussion. Firstly immunostainers. We are a small research lab and hence I'm interested in a machine that is totally flexible with regards to running several antibodies with 2 /3 different methods. Ideally an "open system" that will also dewax & retrieve if possible. We've looked at dako and biogenix which are both open systems and ventana and vision biosytems. Any comments on the above, or on any other systems available would be great. I know this subject ha been covered several times on the histonet, but an up to date view would be much appreciated. Secondly has anybody had any problems with regards to TMA sections falling off slides. We use 1mm cores and only put about 100 in each block, which are then cut and placed on APES coated slides. Sometimes up to 30% of the core may come off. I think next we'll try charged slides. Does anybody have any opinions / ideas on this Many thanks in advance for any opinions. Garry Ashton PICR UK -------------------------------------------------------- This email is confidential and intended solely for the use of the person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author and do not necessarily represent those of the Paterson Institute for Cancer Research or the University of Manchester. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pruegg <@t> ihctech.net Mon Aug 14 10:19:26 2006 From: pruegg <@t> ihctech.net (Patsy Ruegg) Date: Mon Aug 14 10:19:27 2006 Subject: [Histonet] contaminants in tissue processor In-Reply-To: <144.138.164.171.1155512179@my.monash.edu.au> Message-ID: <200608141519.k7EFJMqX001643@chip.viawest.net> White blooms sounds like fungus. Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pruegg@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kim O'Sullivan Sent: Sunday, August 13, 2006 4:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] contaminants in tissue processor Hi everyone, We have a very old citadel tissue processor that we use to process mouse kidney,liver and joints. We have found recently white blooms (best description I can think of- looks like cotton wool) in the first pot of histosol after the last alcohol- does anyone know what these could be and how to prevent them? Secondly since we have changed from using xylene to histosol we seem to be getting a toffee like substance on the bottom of the wax pots-is there any way we can prevent this? Any suggestions would be helpful KimO'Sullivan _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dfvilleg <@t> mtu.edu Mon Aug 14 10:19:28 2006 From: dfvilleg <@t> mtu.edu (Diego Villegas) Date: Mon Aug 14 10:19:33 2006 Subject: [Histonet] meniscus/tibia attachments histology Message-ID: <44E09480.5030608@mtu.edu> Hi, I am working in the histology of meniscus/tibia attachments. I am looking for the collagen orientation and distribution, and GAGs content of meniscus-midsubstance-bone. I found that bone needs to be decalcified (I use 10% formic acid) before fixed in 10% NBF. My questions are: Could the decalcification affect the structure of midsubstance and meniscus?. Do I need to put the bone after decalcification in ammonia solution or Can I jump this step?. What is the time recommended for washing the samples after decalcification? I would appreciate your advice. Thanks, Diego Villegas Michigan Tech e-mail: dfvilleg@mtu.edu From BMolinari <@t> heart.thi.tmc.edu Mon Aug 14 10:53:41 2006 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Mon Aug 14 10:53:45 2006 Subject: [Histonet] Leica DRS Message-ID: Hi, I am frustrated. I have been struggling to solve this problem and now turn to you. My H&E's come out of the stainer with this blue line across them. I have juggled times, rinses, solutions. The slides are cut at 4-5 microns. This is my current protocol Dryer: 20 min Xylene x3 5 min 100 % x3 1 min 95% 1 min rinse in tap rinse in DH2O Richard Allan hematoxylin 1 1 min ( initially started w/ hematoxylin 2 but staining was too dark, I have juggled these times from 15 secs-1 min) Rinse in tap 2 min Richard Allan Clarifier 2 30 secs (have played w/ this time too) Rinse in tap 2 min Richard Allan Bluing 1 min Rinse in tap 2 min 95 % 1 min Richard Allan Eosin Y 45 secs 100%- Xylenes I min. The staining is acceptable..good nuclear staining but still that damn blue streak! Any suggestions? Thanks. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) From BMolinari <@t> heart.thi.tmc.edu Mon Aug 14 11:13:39 2006 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Mon Aug 14 11:13:42 2006 Subject: [Histonet] Leica DRS In-Reply-To: <1155571562_22939@mail.serverlogistics.com> Message-ID: No, I haven't tried that yet. But I sure will. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: Donna Willis [mailto:donna@milestonemed.com] Sent: Monday, August 14, 2006 11:06 AM To: Molinari, Betsy Subject: RE: [Histonet] Leica DRS Betsy, When I was the Lab Manager at Harris we had the same problem with our Sakura stainer. Have you tried stirring up your paraffin before you embed. It seems that the polymers can separate if they set too long. Try and see if this helps. If you are interested in a demo of the Milestone microwave processors just let me know. Donna Willis,HT(ASCP)HTL Milestone Medical North American Application Manager Gulf Coast Sales 2100 N. Hwy 360 Suite 506 Grand Prairie, Tx 75050 972-606-9986 office 214-725-6184 cell -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Monday, August 14, 2006 10:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica DRS Hi, I am frustrated. I have been struggling to solve this problem and now turn to you. My H&E's come out of the stainer with this blue line across them. I have juggled times, rinses, solutions. The slides are cut at 4-5 microns. This is my current protocol Dryer: 20 min Xylene x3 5 min 100 % x3 1 min 95% 1 min rinse in tap rinse in DH2O Richard Allan hematoxylin 1 1 min ( initially started w/ hematoxylin 2 but staining was too dark, I have juggled these times from 15 secs-1 min) Rinse in tap 2 min Richard Allan Clarifier 2 30 secs (have played w/ this time too) Rinse in tap 2 min Richard Allan Bluing 1 min Rinse in tap 2 min 95 % 1 min Richard Allan Eosin Y 45 secs 100%- Xylenes I min. The staining is acceptable..good nuclear staining but still that damn blue streak! Any suggestions? Thanks. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> heart.thi.tmc.edu Mon Aug 14 11:14:57 2006 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Mon Aug 14 11:15:00 2006 Subject: FW: [Histonet] Leica DRS Message-ID: Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: Molinari, Betsy Sent: Monday, August 14, 2006 11:14 AM To: 'Donna Willis'; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Leica DRS No, I haven't tried that yet. But I sure will. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: Donna Willis [mailto:donna@milestonemed.com] Sent: Monday, August 14, 2006 11:06 AM To: Molinari, Betsy Subject: RE: [Histonet] Leica DRS Betsy, When I was the Lab Manager at Harris we had the same problem with our Sakura stainer. Have you tried stirring up your paraffin before you embed. It seems that the polymers can separate if they set too long. Try and see if this helps. If you are interested in a demo of the Milestone microwave processors just let me know. Donna Willis,HT(ASCP)HTL Milestone Medical North American Application Manager Gulf Coast Sales 2100 N. Hwy 360 Suite 506 Grand Prairie, Tx 75050 972-606-9986 office 214-725-6184 cell -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Monday, August 14, 2006 10:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica DRS Hi, I am frustrated. I have been struggling to solve this problem and now turn to you. My H&E's come out of the stainer with this blue line across them. I have juggled times, rinses, solutions. The slides are cut at 4-5 microns. This is my current protocol Dryer: 20 min Xylene x3 5 min 100 % x3 1 min 95% 1 min rinse in tap rinse in DH2O Richard Allan hematoxylin 1 1 min ( initially started w/ hematoxylin 2 but staining was too dark, I have juggled these times from 15 secs-1 min) Rinse in tap 2 min Richard Allan Clarifier 2 30 secs (have played w/ this time too) Rinse in tap 2 min Richard Allan Bluing 1 min Rinse in tap 2 min 95 % 1 min Richard Allan Eosin Y 45 secs 100%- Xylenes I min. The staining is acceptable..good nuclear staining but still that damn blue streak! Any suggestions? Thanks. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> heart.thi.tmc.edu Mon Aug 14 11:17:50 2006 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Mon Aug 14 11:17:53 2006 Subject: [Histonet] Leica DRS In-Reply-To: Message-ID: Yes, this happens consistently. When I filter or start fresh. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: Douglas D Deltour [mailto:doug@ppspath.com] Sent: Monday, August 14, 2006 11:17 AM To: Molinari, Betsy Subject: RE: [Histonet] Leica DRS Do you filter the Hematoxylin? Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Monday, August 14, 2006 11:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica DRS Hi, I am frustrated. I have been struggling to solve this problem and now turn to you. My H&E's come out of the stainer with this blue line across them. I have juggled times, rinses, solutions. The slides are cut at 4-5 microns. This is my current protocol Dryer: 20 min Xylene x3 5 min 100 % x3 1 min 95% 1 min rinse in tap rinse in DH2O Richard Allan hematoxylin 1 1 min ( initially started w/ hematoxylin 2 but staining was too dark, I have juggled these times from 15 secs-1 min) Rinse in tap 2 min Richard Allan Clarifier 2 30 secs (have played w/ this time too) Rinse in tap 2 min Richard Allan Bluing 1 min Rinse in tap 2 min 95 % 1 min Richard Allan Eosin Y 45 secs 100%- Xylenes I min. The staining is acceptable..good nuclear staining but still that damn blue streak! Any suggestions? Thanks. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> heart.thi.tmc.edu Mon Aug 14 11:19:26 2006 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Mon Aug 14 11:19:32 2006 Subject: [Histonet] Leica DRS In-Reply-To: <2FDC4BEE974F1E44A525F9E3DB9A9EC2C51421@mercury.warrenhospital.org> Message-ID: Get rid of all tap or just the tap rinses after hematoxylin? Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: Terri Gillow [mailto:TerriGillow@warrenhospital.org] Sent: Monday, August 14, 2006 11:03 AM To: Molinari, Betsy Subject: RE: [Histonet] Leica DRS Betsy, I feel your pain. The only way we finally got rid of the blue line (that caused a lot of blues) is we started using Distilled H2O in place of tap water. It has never been back. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Monday, August 14, 2006 11:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica DRS Hi, I am frustrated. I have been struggling to solve this problem and now turn to you. My H&E's come out of the stainer with this blue line across them. I have juggled times, rinses, solutions. The slides are cut at 4-5 microns. This is my current protocol Dryer: 20 min Xylene x3 5 min 100 % x3 1 min 95% 1 min rinse in tap rinse in DH2O Richard Allan hematoxylin 1 1 min ( initially started w/ hematoxylin 2 but staining was too dark, I have juggled these times from 15 secs-1 min) Rinse in tap 2 min Richard Allan Clarifier 2 30 secs (have played w/ this time too) Rinse in tap 2 min Richard Allan Bluing 1 min Rinse in tap 2 min 95 % 1 min Richard Allan Eosin Y 45 secs 100%- Xylenes I min. The staining is acceptable..good nuclear staining but still that damn blue streak! Any suggestions? Thanks. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tissuearray <@t> hotmail.com Mon Aug 14 11:21:04 2006 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Mon Aug 14 11:21:15 2006 Subject: [Histonet] autostainers & TMA's Message-ID: I would not recommend the Tape Transfer System for paraffin blocks. The sections are not near the quality of regular micronomy (ribboning and using a water bath). We have stained hundreds of TMA slides on our Dako and Vantana systems and have not had a problem with cores falling off. If you use plus slides you should not have problems. Just make sure the slides are dried well. Thom Jensen For information on problems with the Tape Sectioning Method go to: http://arrayworkshop.com/Notes_2.html >From: "Helen Fedor" >To: , >Subject: Re: [Histonet] autostainers & TMA's >Date: Mon, 14 Aug 2006 09:53:25 -0400 >MIME-Version: 1.0 >Received: from swlx162.swmed.edu ([199.165.152.162]) by >bay0-mc4-f17.bay0.hotmail.com with Microsoft SMTPSVC(6.0.3790.2444); Mon, >14 Aug 2006 06:57:20 -0700 >Received: from localhost ([127.0.0.1] helo=swlx162.swmed.edu)by >swlx162.swmed.edu with esmtp (Exim 4.34)id 1GCct0-0005Rb-7O; Mon, 14 Aug >2006 08:53:46 -0500 >Received: from [199.165.152.167] (helo=swlx167.swmed.edu)by >swlx162.swmed.edu with esmtp (Exim 4.34) id 1GCcsx-0005RU-1Lfor >histonet@lists.utsouthwestern.edu; Mon, 14 Aug 2006 08:53:44 -0500 >Received: from ipex2.johnshopkins.edu ([162.129.8.151])by swlx167.swmed.edu >with esmtp (Exim 4.44) id 1GCcsv-0001tX-QCfor >histonet@lists.utsouthwestern.edu; Mon, 14 Aug 2006 08:53:42 -0500 >Received: from jhuadig.admin.jhu.edu (HELO >cis27.hosts.jhmi.edu)([10.15.69.48]) by ipex2.johnshopkins.edu with >ESMTP/TLS/AES256-SHA;14 Aug 2006 09:53:37 -0400 >Received: from Jhmipri-MTA by cis27.hosts.jhmi.eduwith Novell_GroupWise; >Mon, 14 Aug 2006 09:53:36 -0400 >X-Message-Info: LsUYwwHHNt0gGT/wSCJIQE3rHsi6SWysOVcgIhg9xfs= >X-BrightmailFiltered: true >X-Brightmail-Tracker: AAAAAA== >X-IronPort-AV: i="4.08,121,1154923200"; d="scan'208"; >a="184077952:sNHT2665066158" >X-Mailer: Novell GroupWise Internet Agent 7.0.1 References: > >X-Scan-Signature: 9b45ccbbf9b6b65cb3d07f69825436b9 >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.1.5 >Precedence: list >List-Id: For the exchange of information pertaining to histotechnology >andrelated fields >List-Unsubscribe: >, > >List-Archive: >List-Post: >List-Help: >List-Subscribe: >, >Errors-To: histonet-bounces@lists.utsouthwestern.edu >X-Scan-Signature: 5ce762e8aa474ea7aaa37532c083de4e >X-SA-Exim-Connect-IP: 127.0.0.1 >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >X-Spam-Checker-Version: SpamAssassin 2.64 (2004-01-11) on swlx162.swmed.edu >X-Spam-Level: X-Spam-Status: No, hits=0.0 required=5.5 tests=none >autolearn=no version=2.64 >X-SA-Exim-Version: 4.0 (built Wed, 07 Apr 2004 12:14:41 -0500) >X-SA-Exim-Scanned: Yes (on swlx162.swmed.edu) >Return-Path: histonet-bounces@lists.utsouthwestern.edu >X-OriginalArrivalTime: 14 Aug 2006 13:57:20.0410 (UTC) >FILETIME=[8F792FA0:01C6BFA9] > >Sorry, for the last sentence. >It should be: We do not experience tissue loss with this method. >Helen > > >>> Garry Ashton 8/12/2006 8:38 AM >>> >Dear histonetters >I have 2 topics up for discussion. > >Firstly immunostainers. >We are a small research lab and hence I'm interested in a machine that >is totally flexible with regards to running several antibodies with 2 >/3 >different methods. >Ideally an "open system" that will also dewax & retrieve if possible. >We've looked at dako and biogenix which are both open systems and >ventana and vision biosytems. >Any comments on the above, or on any other systems available would be >great. >I know this subject ha been covered several times on the histonet, but >an up to date view would be much appreciated. > >Secondly has anybody had any problems with regards to TMA sections >falling off slides. >We use 1mm cores and only put about 100 in each block, which are then >cut and placed on APES coated slides. >Sometimes up to 30% of the core may come off. >I think next we'll try charged slides. Does anybody have any opinions >/ >ideas on this > >Many thanks in advance for any opinions. > > > > > >Garry Ashton >PICR >UK > >-------------------------------------------------------- > > >This email is confidential and intended solely for the use of the >person(s) ('the intended recipient') to whom it was addressed. Any views >or opinions presented are solely those of the author and do not >necessarily represent those of the Paterson Institute for Cancer >Research or the University of Manchester. It may contain information >that is privileged & confidential within the meaning of applicable law. >Accordingly any dissemination, distribution, copying, or other use of >this message, or any of its contents, by any person other than the >intended recipient may constitute a breach of civil or criminal law and >is strictly prohibited. If you are NOT the intended recipient please >contact the sender and dispose of this e-mail as soon as possible. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> heart.thi.tmc.edu Mon Aug 14 11:22:11 2006 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Mon Aug 14 11:22:14 2006 Subject: [Histonet] Leica DRS In-Reply-To: <000701c6bfbd$1a7f91d0$3601a8c0@brownpathology.net> Message-ID: Bonnie, It is horizontal. I thought the same thing..reagent depth..so I sat and watched the entire run to be sure the basket/slides were submerged and they were. I truly appreciated this help, it is frustrating to be looking at another tray of slides w/ streaks. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: Bonnie Whitaker [mailto:bwhitaker@brownpathology.com] Sent: Monday, August 14, 2006 11:17 AM To: Molinari, Betsy Subject: RE: [Histonet] Leica DRS Betsy, Where is the blue streak? Is it vertical or horizontal? Is it always the same place? I'm wondering if it's a depth of reagent issue (tissue isn't totally submersed in all steps). Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Monday, August 14, 2006 10:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica DRS Hi, I am frustrated. I have been struggling to solve this problem and now turn to you. My H&E's come out of the stainer with this blue line across them. I have juggled times, rinses, solutions. The slides are cut at 4-5 microns. This is my current protocol Dryer: 20 min Xylene x3 5 min 100 % x3 1 min 95% 1 min rinse in tap rinse in DH2O Richard Allan hematoxylin 1 1 min ( initially started w/ hematoxylin 2 but staining was too dark, I have juggled these times from 15 secs-1 min) Rinse in tap 2 min Richard Allan Clarifier 2 30 secs (have played w/ this time too) Rinse in tap 2 min Richard Allan Bluing 1 min Rinse in tap 2 min 95 % 1 min Richard Allan Eosin Y 45 secs 100%- Xylenes I min. The staining is acceptable..good nuclear staining but still that damn blue streak! Any suggestions? Thanks. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Mon Aug 14 11:23:04 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Aug 14 11:23:14 2006 Subject: [Histonet] Leica DRS In-Reply-To: Message-ID: <20060814162305.32655.qmail@web61220.mail.yahoo.com> No tap after the hematoxylin. Ren? J. "Molinari, Betsy" wrote: Get rid of all tap or just the tap rinses after hematoxylin? Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: Terri Gillow [mailto:TerriGillow@warrenhospital.org] Sent: Monday, August 14, 2006 11:03 AM To: Molinari, Betsy Subject: RE: [Histonet] Leica DRS Betsy, I feel your pain. The only way we finally got rid of the blue line (that caused a lot of blues) is we started using Distilled H2O in place of tap water. It has never been back. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Monday, August 14, 2006 11:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica DRS Hi, I am frustrated. I have been struggling to solve this problem and now turn to you. My H&E's come out of the stainer with this blue line across them. I have juggled times, rinses, solutions. The slides are cut at 4-5 microns. This is my current protocol Dryer: 20 min Xylene x3 5 min 100 % x3 1 min 95% 1 min rinse in tap rinse in DH2O Richard Allan hematoxylin 1 1 min ( initially started w/ hematoxylin 2 but staining was too dark, I have juggled these times from 15 secs-1 min) Rinse in tap 2 min Richard Allan Clarifier 2 30 secs (have played w/ this time too) Rinse in tap 2 min Richard Allan Bluing 1 min Rinse in tap 2 min 95 % 1 min Richard Allan Eosin Y 45 secs 100%- Xylenes I min. The staining is acceptable..good nuclear staining but still that damn blue streak! Any suggestions? Thanks. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. From Barbara_Lentz <@t> dahlchase.com Mon Aug 14 13:09:22 2006 From: Barbara_Lentz <@t> dahlchase.com (Barbara Lentz) Date: Mon Aug 14 13:09:57 2006 Subject: [Histonet] Leica DRS Message-ID: Hi there, Perhaps it's the depth when originally mounting the tissue on the slide. Do you use some sort of Sta-On in your waterbath? It could be the cause of retaining excess hematoxylin. Barb >>> "Molinari, Betsy" 08/14/06 12:22PM >>> Bonnie, It is horizontal. I thought the same thing..reagent depth..so I sat and watched the entire run to be sure the basket/slides were submerged and they were. I truly appreciated this help, it is frustrating to be looking at another tray of slides w/ streaks. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: Bonnie Whitaker [mailto:bwhitaker@brownpathology.com] Sent: Monday, August 14, 2006 11:17 AM To: Molinari, Betsy Subject: RE: [Histonet] Leica DRS Betsy, Where is the blue streak? Is it vertical or horizontal? Is it always the same place? I'm wondering if it's a depth of reagent issue (tissue isn't totally submersed in all steps). Bonnie Whitaker -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Monday, August 14, 2006 10:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica DRS Hi, I am frustrated. I have been struggling to solve this problem and now turn to you. My H&E's come out of the stainer with this blue line across them. I have juggled times, rinses, solutions. The slides are cut at 4-5 microns. This is my current protocol Dryer: 20 min Xylene x3 5 min 100 % x3 1 min 95% 1 min rinse in tap rinse in DH2O Richard Allan hematoxylin 1 1 min ( initially started w/ hematoxylin 2 but staining was too dark, I have juggled these times from 15 secs-1 min) Rinse in tap 2 min Richard Allan Clarifier 2 30 secs (have played w/ this time too) Rinse in tap 2 min Richard Allan Bluing 1 min Rinse in tap 2 min 95 % 1 min Richard Allan Eosin Y 45 secs 100%- Xylenes I min. The staining is acceptable..good nuclear staining but still that damn blue streak! Any suggestions? Thanks. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Mon Aug 14 13:11:33 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Aug 14 13:11:40 2006 Subject: Blue line woes RE: [Histonet] Leica DRS In-Reply-To: <20060814162305.32655.qmail@web61220.mail.yahoo.com> References: <20060814162305.32655.qmail@web61220.mail.yahoo.com> Message-ID: <6.0.0.22.1.20060814115510.01b2cd10@gemini.msu.montana.edu> Betsy, Question, not sure why? but is this occuring with freshly changed hematoxylin also? My RA rep said to pour into staining dish without filtering, but I have tried this - and I see all kinds of gunk on my slides, so I went back to filtering into dish, and feel more secure the blues are gone. We have used running tap water after hematoxylin for over 26 years, but we also live in a part of the USA (Montana) where our water is relatively pure, little chlorine, and we never have problems with quality of hematoxylin staining no matter what type of hematoxylin we use. I would assume if you use distilled water for rinsing, you would have to change it for each run of slides, since it is a static, non-flowing rinse unless suggestors (people doing this) have a flowing distilled water setup. Our rehydration is similar to yours, although we go to 70% alcohol then distilled before RA Hematoxylin. We use RA hematoxylin 1 - 1.5 min Tap water rinse 1 min and the water flows from the bottom of slides up over the top, and the hematoxylin is always cleared away nicely before the next step. Clarifier 1 - 1 min - this is changed daily with big runs Tap water rinse 1 min bluing 1 min this is changed daily with big runs tap water 1 min 70% alcohol 1 min in place of 95% alcohcol then proceed to eosin No background on slides, NOR blue lines, we stain manually and it doesn't make any difference what hematoxylin we use Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 At 10:23 AM 8/14/2006, you wrote: >No tap after the hematoxylin. > Ren? J. > >"Molinari, Betsy" wrote: > Get rid of all tap or just the tap rinses after hematoxylin? > >Betsy Molinari HT (ASCP) >Texas Heart Institute >Cardiovascular Pathology >6770 Bertner Ave. >Houston,TX 77030 >832-355-6524 >832-355-6812 (fax) > > >-----Original Message----- >From: Terri Gillow [mailto:TerriGillow@warrenhospital.org] >Sent: Monday, August 14, 2006 11:03 AM >To: Molinari, Betsy >Subject: RE: [Histonet] Leica DRS > >Betsy, >I feel your pain. The only way we finally got rid of the blue line >(that caused a lot of blues) is we started using Distilled H2O in place >of tap water. It has never been back. > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of >Molinari, Betsy >Sent: Monday, August 14, 2006 11:54 AM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Leica DRS > >Hi, > >I am frustrated. I have been struggling to solve this problem and now >turn to you. > >My H&E's come out of the stainer with this blue line across them. I >have juggled times, rinses, solutions. > >The slides are cut at 4-5 microns. This is my current protocol > >Dryer: 20 min > >Xylene x3 5 min > >100 % x3 1 min > >95% 1 min > >rinse in tap > >rinse in DH2O > >Richard Allan hematoxylin 1 1 min ( initially started w/ hematoxylin 2 >but staining was too dark, I have juggled these times from 15 secs-1 >min) > >Rinse in tap 2 min > >Richard Allan Clarifier 2 30 secs (have played w/ this time too) > >Rinse in tap 2 min > >Richard Allan Bluing 1 min > >Rinse in tap 2 min > >95 % 1 min > >Richard Allan Eosin Y 45 secs > >100%- Xylenes I min. > >The staining is acceptable..good nuclear staining but still that damn >blue streak! Any suggestions? > >Thanks. > > > >Betsy Molinari HT (ASCP) > >Texas Heart Institute > >Cardiovascular Pathology > >6770 Bertner Ave. > >Houston,TX 77030 > >832-355-6524 > >832-355-6812 (fax) > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great >rates starting at 1?/min. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From BMolinari <@t> heart.thi.tmc.edu Mon Aug 14 13:22:21 2006 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Mon Aug 14 13:22:25 2006 Subject: [Histonet] Leica DRS In-Reply-To: <0556BE8AC5551E4E8AF6BB9E42509BA2048F7254@usca0082k08.labvision.apogent.com> Message-ID: You know what..DUH! I the stripe is vertical. I did not consider how the slide is positioned while in the stainer which is vertical. So the drop running down the slide sounds sensible. How limited was my perception of this problem? OK..fresh eyes..new ideas..thanks! Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: Morken, Tim - Labvision [mailto:tpmorken@labvision.com] Sent: Monday, August 14, 2006 11:31 AM To: Molinari, Betsy Subject: RE: [Histonet] Leica DRS Betsy, you don't say which direction the streak goes, but I am guessing it is vertical (on the long axis of the slide). If this is the case I suggest you look at the level of your eosin compared to the 95% just before it. We had this problem once and discovered the eosin level was lower than the 95% level in the previous bath. Then when the slide went into eosin it did not rinse off the 95% at the top and left a drop above the level of the eosin. Then when the slide came out of the eosin that drop would slide down the middle of the slide and wash out some of the eosin before going into the next alcohol. It doesn't sound like much, but was enough to make a "blue" streak down the slide. It was really some of the eosin washing out. Tim Morken Lab Vision - Neomarkers www.labvision.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Monday, August 14, 2006 8:54 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Leica DRS Hi, I am frustrated. I have been struggling to solve this problem and now turn to you. My H&E's come out of the stainer with this blue line across them. I have juggled times, rinses, solutions. The slides are cut at 4-5 microns. This is my current protocol Dryer: 20 min Xylene x3 5 min 100 % x3 1 min 95% 1 min rinse in tap rinse in DH2O Richard Allan hematoxylin 1 1 min ( initially started w/ hematoxylin 2 but staining was too dark, I have juggled these times from 15 secs-1 min) Rinse in tap 2 min Richard Allan Clarifier 2 30 secs (have played w/ this time too) Rinse in tap 2 min Richard Allan Bluing 1 min Rinse in tap 2 min 95 % 1 min Richard Allan Eosin Y 45 secs 100%- Xylenes I min. The staining is acceptable..good nuclear staining but still that damn blue streak! Any suggestions? Thanks. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From lrichey <@t> u.washington.edu Mon Aug 14 14:05:49 2006 From: lrichey <@t> u.washington.edu (Lori Richey) Date: Mon Aug 14 14:06:02 2006 Subject: [Histonet] Rapid Tissue Processor In-Reply-To: <8309429.1155501927975.JavaMail.root@webmail6> References: <8309429.1155501927975.JavaMail.root@webmail6> Message-ID: <44E0C98D.5000505@u.washington.edu> My suggestion is to run comparative studies. Fix the same tissue in formalin, and the rapid fixative, and run a comparison on a conventional processor. You may not want to use the rapid fixative, and continue to use formalin. There may be nothing you can do about the staining artifact in certain tissues. Some tissues may be acceptable and some may not. The pathologists need to see a comparison for every tissue type in order to make their decision about the use of the processor. We still only run small biopsies, excluding breast, skin and renal. Everything is fixed in formalin. a.schmidt@fuse.net wrote: >To All, > We have the new Sakura Rapid Tissue Processor and I could really use some feedback! We seem to be having trouble with inconsistencies in fixation--staining artifact and all sorts of things! We were processing about 75% of our surgical specimens on it and have since cut back to just small biopsies because of pathologist complaints. Help---any suggestions as what worked for you!! Sakura is coming in this week to help us too!! > Thanks > Angie Schmidt > Tri-Health Labs--Cincinnati,Ohio > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From histosci <@t> shentel.net Mon Aug 14 14:40:59 2006 From: histosci <@t> shentel.net (HSRL) Date: Mon Aug 14 14:41:12 2006 Subject: [Histonet] Leica microtomes for plastics Message-ID: <000a01c6bfd9$94c2f150$0500a8c0@HSRLMAIN> Dear Vendors, We are looking to purchase a couple of microtomes suitable for both MMA and GMA sectioning with a tungsten carbide knife. Do any vendors have any used/demod LEICA microtomes they would like to sell? Please note: We are only interested in Leica at this time. Please e-mail me with inventory and prices. I look forward to hearing from you. -Tom Tom Galati Laboratory Director HSRL, Inc.- A GLP Compliant Contract Laboratory 5930 Main Street Mount Jackson, Virginia 22842 540.477.4440 540.477.4448 tomgalati@hsrl.org www.hsrl.org From parkiepark <@t> gmail.com Mon Aug 14 20:15:41 2006 From: parkiepark <@t> gmail.com (Soyoung Park) Date: Mon Aug 14 20:17:23 2006 Subject: [Histonet] nissl re-staining Message-ID: Hi, I just started histology in June, and I'm currently working with 60~80-micron-thick brain tissues. My first few batches of thionin stain came out extremely light, making it impossible for me to perform stereology on those sections. So I'm trying to find a way to re-stain them but so far I haven't been able to acquire a good protocol. Could anyone help me with this? Another thing, I figured out the right amount of time to keep the tissues in thionin, and I've been able to get some purple (pretty) sections. But often, instead of purple, I get something closer to dark blue, which my PI thinks is not right. To me it seems like xylene or Histoclear is doing something, becaue the sections look okay until I put them in xylene, then in Histoclear. Does anyone know what this is about? Will I be able to fix this by re-staing? Thank you. -- Soyoung Park Biology '09 California Institute of Technology MSC 769 Pasadena, CA From ree3 <@t> leicester.ac.uk Tue Aug 15 06:39:28 2006 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Tue Aug 15 06:39:34 2006 Subject: [Histonet] Choline kinase In-Reply-To: <09C945920A6B654199F7A58A1D7D1FDE0171777D@lsexch.lsmaster.lifespan.org> Message-ID: Anybody aware of an antibody to choline kinase that works on paraffin processed mouse tissues??many thanks. Richard Edwards MRC TOXICOLOGY UNIT LEICESTER..U.K.... From godsgirlnow <@t> msn.com Tue Aug 15 07:49:55 2006 From: godsgirlnow <@t> msn.com (Roxanne Soto) Date: Tue Aug 15 07:50:04 2006 Subject: [Histonet] JOB IN TAMPA AVAILABLE Message-ID: Hello everyone....Just wanted to let you know that I have a few openings for Histotechs and for cytotechs in Tampa area (near Busch Gardens). I am looking for cytotechs with FISH expereince and for Histotechnologists willing to learn as we are on the verge of molecular histology here. Please email me directly. Roxanne Soto HT(ASCP)QIHC Lab Manager Physicians RightPath 813-549-1050 [1]rsoto@physiciansrightpath.com References 1. mailto:rsoto@physiciansrightpath.com From chiggerson <@t> memhosp.com Tue Aug 15 08:37:01 2006 From: chiggerson <@t> memhosp.com (chiggerson@memhosp.com) Date: Tue Aug 15 08:37:14 2006 Subject: [Histonet] Two patient identifiers and handwritten blocks Message-ID: For those of you that are still handwriting accession numbers on your blocks: 1. Do you require both the accession number and the patient's name to be handwritten on the cassettes to meet the two patient identifier patient safety requirement? 2. If so, do you require the patient's full name? Or last name, first initial? 3. If not using the patient's name as the second identifier, what are you using? Medical Record Number? 4. If not using two patient identifiers (accession number only), how are you justifying not using two identifiers to meet the patient safety requirements? 5. For those that have implemented adding a second handwritten identifier to blocks, how did your staff react? The obvious solution is a cassette labeler, however it just is not going to happen soon. Thank you in advance for your response to this very important issue. Thanks, Cindy From sbreeden <@t> nmda.nmsu.edu Tue Aug 15 10:58:08 2006 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Tue Aug 15 10:58:19 2006 Subject: [Histonet] Histology & Baseball Message-ID: For those of you who are baseball-loving histologists and you responded to my post last month about seeing a Diamondbacks game while in PHX at NSH, please contact me again. I will assemble a list of Interested Parties and get in touch with the AZ NSH local committee to see what we can do. Reply to this email. Sally Breeden NM Dept of Agriculture Veterinary Diagnostic Services POBox 700 Albuquerque, NM 87106 505-841-2576 From lblazek <@t> digestivespecialists.com Tue Aug 15 11:11:49 2006 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Tue Aug 15 11:07:05 2006 Subject: [Histonet] Histology & Baseball Message-ID: <6CBA6DC98A079D408C87250591D9DFB802684A1F@bruexchange.digestivespecialists.com> Count me in! Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Tuesday, August 15, 2006 11:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histology & Baseball For those of you who are baseball-loving histologists and you responded to my post last month about seeing a Diamondbacks game while in PHX at NSH, please contact me again. I will assemble a list of Interested Parties and get in touch with the AZ NSH local committee to see what we can do. Reply to this email. Sally Breeden NM Dept of Agriculture Veterinary Diagnostic Services POBox 700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From chiggerson <@t> memhosp.com Tue Aug 15 11:21:40 2006 From: chiggerson <@t> memhosp.com (chiggerson@memhosp.com) Date: Tue Aug 15 11:21:56 2006 Subject: [Histonet] Two patient identifiers and handwritten blocks In-Reply-To: Message-ID: Okay, so I do not receive any more "flaming" and vicious e-mails, let me clarify. JCAHO requires two patient identifiers when collecting specimens, administering meds, etc. JCAHO recommends and encourages laboratories to use two patient identifiers on all aliquots (this includes blocks and slides). Our institution has implemented a two patient identifier policy before taking any action with a patient, patient specimen, or patient record (NOT just specimen collection). This is institution wide. In addition, the laboratory has implemented the two patient identifier policy for all testing phases including preanalytical, analytical and postanalytical for both specimens and records. This is not only recommended by JCAHO but also recommended by CAP. Please note it is currently a recommendation, not a requirement. If you need a reference, refer to CAP's publication "Quality Management in Anatomic Pathology". Recommendations for including two patient identifiers on both frozen section slides and on all blocks and slides is located in the chapter "Strategies for Error Reduction and Prevention in Surgical Pathology". More and more institutions are taking these recommendatioins seriously and implementing policies both institution wide and laboratory wide. Mine is such a place. We have always used two patient identifiers, except on the blocks. Therefore, for those that are taking this seriously, I would like to know which two patient identifiers are used and how you implemented it. I NEED to comply with my institutional and laboratory's policies. Thanks, Cindy "Richard Yeo" 08/15/2006 10:31 AM To cc Subject RE: [Histonet] Two patient identifiers and handwritten blocks SPECIMENS COMING INTO THE LAB REQUIRE TWO PATIENT IDENTIFIERS, NAME/DATE OF BIRTH/SSN#... NOT PARAFFIN BLOCKS/SLIDES. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of chiggerson@memhosp.com Sent: Tuesday, August 15, 2006 9:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Two patient identifiers and handwritten blocks For those of you that are still handwriting accession numbers on your blocks: 1. Do you require both the accession number and the patient's name to be handwritten on the cassettes to meet the two patient identifier patient safety requirement? 2. If so, do you require the patient's full name? Or last name, first initial? 3. If not using the patient's name as the second identifier, what are you using? Medical Record Number? 4. If not using two patient identifiers (accession number only), how are you justifying not using two identifiers to meet the patient safety requirements? 5. For those that have implemented adding a second handwritten identifier to blocks, how did your staff react? The obvious solution is a cassette labeler, however it just is not going to happen soon. Thank you in advance for your response to this very important issue. Thanks, Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JEllin <@t> yumaregional.org Tue Aug 15 11:43:10 2006 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Tue Aug 15 11:43:26 2006 Subject: [Histonet] Two patient identifiers and handwritten blocks Message-ID: <29BE166A2CF48D459853F8EC57CD37E83ABF82@EXCHANGECLUSTER.yumaregional.local> Could one of the Identifiers be a 2D barcode or just a plain barcode. This is generted specificly for this block with this patients information. That could be used as a identifier, or does it have to be something that is a readable identifer to everyone that is handling that case.?? Jesus A. Ellin HT ASCP Yuma Regional Medical Center Histology Systems Technologist Pathology Information Systems 928-336-7444 or 928-336-1144 Fax: 928-336-7319 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of chiggerson@memhosp.com Sent: Tuesday, August 15, 2006 9:22 AM To: Richard Yeo; Blazek, Linda Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Two patient identifiers and handwritten blocks Okay, so I do not receive any more "flaming" and vicious e-mails, let me clarify. JCAHO requires two patient identifiers when collecting specimens, administering meds, etc. JCAHO recommends and encourages laboratories to use two patient identifiers on all aliquots (this includes blocks and slides). Our institution has implemented a two patient identifier policy before taking any action with a patient, patient specimen, or patient record (NOT just specimen collection). This is institution wide. In addition, the laboratory has implemented the two patient identifier policy for all testing phases including preanalytical, analytical and postanalytical for both specimens and records. This is not only recommended by JCAHO but also recommended by CAP. Please note it is currently a recommendation, not a requirement. If you need a reference, refer to CAP's publication "Quality Management in Anatomic Pathology". Recommendations for including two patient identifiers on both frozen section slides and on all blocks and slides is located in the chapter "Strategies for Error Reduction and Prevention in Surgical Pathology". More and more institutions are taking these recommendatioins seriously and implementing policies both institution wide and laboratory wide. Mine is such a place. We have always used two patient identifiers, except on the blocks. Therefore, for those that are taking this seriously, I would like to know which two patient identifiers are used and how you implemented it. I NEED to comply with my institutional and laboratory's policies. Thanks, Cindy "Richard Yeo" 08/15/2006 10:31 AM To cc Subject RE: [Histonet] Two patient identifiers and handwritten blocks SPECIMENS COMING INTO THE LAB REQUIRE TWO PATIENT IDENTIFIERS, NAME/DATE OF BIRTH/SSN#... NOT PARAFFIN BLOCKS/SLIDES. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of chiggerson@memhosp.com Sent: Tuesday, August 15, 2006 9:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Two patient identifiers and handwritten blocks For those of you that are still handwriting accession numbers on your blocks: 1. Do you require both the accession number and the patient's name to be handwritten on the cassettes to meet the two patient identifier patient safety requirement? 2. If so, do you require the patient's full name? Or last name, first initial? 3. If not using the patient's name as the second identifier, what are you using? Medical Record Number? 4. If not using two patient identifiers (accession number only), how are you justifying not using two identifiers to meet the patient safety requirements? 5. For those that have implemented adding a second handwritten identifier to blocks, how did your staff react? The obvious solution is a cassette labeler, however it just is not going to happen soon. Thank you in advance for your response to this very important issue. Thanks, Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. From chiggerson <@t> memhosp.com Tue Aug 15 11:57:41 2006 From: chiggerson <@t> memhosp.com (chiggerson@memhosp.com) Date: Tue Aug 15 11:57:56 2006 Subject: [Histonet] Two patient identifiers and handwritten blocks In-Reply-To: <29BE166A2CF48D459853F8EC57CD37E83ABF82@EXCHANGECLUSTER.yumaregional.local> Message-ID: Good question! I think the preferred solution is a barcode that is linked to the patient's information. I am not sure if user readability is required. I would think not if you are using bar code readers at embedding and microtomy like some larger institutions are doing. I think MGH implemented such a system. They scan their block just before microtomy and the labels for that block print right at the microtome. You can't get better positive patient ID than that! I assume that the accession number is also on the block because you still have to file and retrieve it. It would be interesting to find out if they are using just the bar code and acession number, or if they also have patient name. Is there any one from MGH out there that can answer this? Thanks, Cindy "Jesus Ellin" 08/15/2006 11:43 AM To chiggerson@memhosp.com, "Richard Yeo" , "Blazek, Linda" cc histonet@lists.utsouthwestern.edu Subject RE: [Histonet] Two patient identifiers and handwritten blocks Could one of the Identifiers be a 2D barcode or just a plain barcode. This is generted specificly for this block with this patients information. That could be used as a identifier, or does it have to be something that is a readable identifer to everyone that is handling that case.?? Jesus A. Ellin HT ASCP Yuma Regional Medical Center Histology Systems Technologist Pathology Information Systems 928-336-7444 or 928-336-1144 Fax: 928-336-7319 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of chiggerson@memhosp.com Sent: Tuesday, August 15, 2006 9:22 AM To: Richard Yeo; Blazek, Linda Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Two patient identifiers and handwritten blocks Okay, so I do not receive any more "flaming" and vicious e-mails, let me clarify. JCAHO requires two patient identifiers when collecting specimens, administering meds, etc. JCAHO recommends and encourages laboratories to use two patient identifiers on all aliquots (this includes blocks and slides). Our institution has implemented a two patient identifier policy before taking any action with a patient, patient specimen, or patient record (NOT just specimen collection). This is institution wide. In addition, the laboratory has implemented the two patient identifier policy for all testing phases including preanalytical, analytical and postanalytical for both specimens and records. This is not only recommended by JCAHO but also recommended by CAP. Please note it is currently a recommendation, not a requirement. If you need a reference, refer to CAP's publication "Quality Management in Anatomic Pathology". Recommendations for including two patient identifiers on both frozen section slides and on all blocks and slides is located in the chapter "Strategies for Error Reduction and Prevention in Surgical Pathology". More and more institutions are taking these recommendatioins seriously and implementing policies both institution wide and laboratory wide. Mine is such a place. We have always used two patient identifiers, except on the blocks. Therefore, for those that are taking this seriously, I would like to know which two patient identifiers are used and how you implemented it. I NEED to comply with my institutional and laboratory's policies. Thanks, Cindy "Richard Yeo" 08/15/2006 10:31 AM To cc Subject RE: [Histonet] Two patient identifiers and handwritten blocks SPECIMENS COMING INTO THE LAB REQUIRE TWO PATIENT IDENTIFIERS, NAME/DATE OF BIRTH/SSN#... NOT PARAFFIN BLOCKS/SLIDES. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of chiggerson@memhosp.com Sent: Tuesday, August 15, 2006 9:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Two patient identifiers and handwritten blocks For those of you that are still handwriting accession numbers on your blocks: 1. Do you require both the accession number and the patient's name to be handwritten on the cassettes to meet the two patient identifier patient safety requirement? 2. If so, do you require the patient's full name? Or last name, first initial? 3. If not using the patient's name as the second identifier, what are you using? Medical Record Number? 4. If not using two patient identifiers (accession number only), how are you justifying not using two identifiers to meet the patient safety requirements? 5. For those that have implemented adding a second handwritten identifier to blocks, how did your staff react? The obvious solution is a cassette labeler, however it just is not going to happen soon. Thank you in advance for your response to this very important issue. Thanks, Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. From victor <@t> pathology.washington.edu Tue Aug 15 12:08:35 2006 From: victor <@t> pathology.washington.edu (Victor Tobias) Date: Tue Aug 15 12:08:43 2006 Subject: [Histonet] Two patient identifiers and handwritten blocks In-Reply-To: References: Message-ID: <44E1FF93.7040300@pathology.washington.edu> In the presentation I saw for MGH, they have the accession number, block ID and barcode. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. chiggerson@memhosp.com wrote: > Good question! I think the preferred solution is a barcode that is linked > to the patient's information. I am not sure if user readability is > required. I would think not if you are using bar code readers at embedding > and microtomy like some larger institutions are doing. I think MGH > implemented such a system. They scan their block just before microtomy > and the labels for that block print right at the microtome. You can't get > better positive patient ID than that! I assume that the accession number > is also on the block because you still have to file and retrieve it. It > would be interesting to find out if they are using just the bar code and > acession number, or if they also have patient name. > > Is there any one from MGH out there that can answer this? > > Thanks, > Cindy > > > > > > "Jesus Ellin" > 08/15/2006 11:43 AM > > To > chiggerson@memhosp.com, "Richard Yeo" , "Blazek, Linda" > > cc > histonet@lists.utsouthwestern.edu > Subject > RE: [Histonet] Two patient identifiers and handwritten blocks > > > > > > > Could one of the Identifiers be a 2D barcode or just a plain barcode. > This is generted specificly for this block with this patients > information. That could be used as a identifier, or does it have to be > something that is a readable identifer to everyone that is handling that > case.?? > > > Jesus A. Ellin HT ASCP > Yuma Regional Medical Center > Histology Systems Technologist > Pathology Information Systems > 928-336-7444 or 928-336-1144 > Fax: 928-336-7319 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > chiggerson@memhosp.com > Sent: Tuesday, August 15, 2006 9:22 AM > To: Richard Yeo; Blazek, Linda > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Two patient identifiers and handwritten blocks > > Okay, so I do not receive any more "flaming" and vicious e-mails, let me > clarify. > > JCAHO requires two patient identifiers when collecting specimens, > administering meds, etc. JCAHO recommends and encourages laboratories to > use two patient identifiers on all aliquots (this includes blocks and > slides). Our institution has implemented a two patient identifier policy > before taking any action with a patient, patient specimen, or patient > record (NOT just specimen collection). This is institution wide. In > addition, the laboratory has implemented the two patient identifier > policy for all testing phases including preanalytical, analytical and > postanalytical for both specimens and records. This is not only > recommended by JCAHO but also recommended by CAP. Please note it is > currently a recommendation, not a requirement. If you need a reference, > refer to CAP's publication "Quality Management in Anatomic Pathology". > Recommendations for including two patient identifiers on both frozen > section slides and on all blocks and slides is located in the chapter > "Strategies for Error Reduction and Prevention in Surgical Pathology". > More and more institutions are taking these recommendatioins seriously > and implementing policies both institution wide and laboratory wide. > Mine is such a place. We have always used two patient identifiers, > except on the blocks. Therefore, for those that are taking this > seriously, I would like to know which two patient identifiers are used > and how you implemented it. > I NEED to comply with my institutional and laboratory's policies. > > > Thanks, > Cindy > > > > > > "Richard Yeo" > 08/15/2006 10:31 AM > > To > > cc > > Subject > RE: [Histonet] Two patient identifiers and handwritten blocks > > > > > > > SPECIMENS COMING INTO THE LAB REQUIRE TWO PATIENT IDENTIFIERS, NAME/DATE > OF BIRTH/SSN#... > NOT PARAFFIN BLOCKS/SLIDES. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > chiggerson@memhosp.com > Sent: Tuesday, August 15, 2006 9:37 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Two patient identifiers and handwritten blocks > > For those of you that are still handwriting accession numbers on your > blocks: > > 1. Do you require both the accession number and the patient's name > to > be handwritten on the cassettes to meet the two patient identifier > patient safety requirement? > > 2. If so, do you require the patient's full name? Or last name, > first > initial? > > 3. If not using the patient's name as the second identifier, what > are > you using? Medical Record Number? > > 4. If not using two patient identifiers (accession number only), > how > are you justifying not using two identifiers to meet the patient safety > requirements? > > 5. For those that have implemented adding a second handwritten > identifier to blocks, how did your staff react? > > The obvious solution is a cassette labeler, however it just is not going > to happen soon. Thank you in advance for your response to this very > important issue. > > Thanks, > Cindy > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > This message is confidential, intended only for the named > recipient(s) and may contain information that is privileged > or exempt from disclosure under applicable law. If you are > not the intended recipient(s), you are notified that the > dissemination, distribution, or copying of this message is > strictly prohibited. If you receive this message in error, > or are not the named recipient(s), please notify the sender > at either the e-mail, fax, address, or telephone number > listed above and delete this e-mail from your computer. > Thank you. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From JWEEMS <@t> sjha.org Tue Aug 15 12:15:31 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Aug 15 12:15:54 2006 Subject: [Histonet] Two patient identifiers and handwritten blocks Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEE657@sjhaexc02.sjha.org> We use the case number and name on the slide label. We hope to have a barcode with our next LIS upgrade. j Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 404-851-7376 - Phone 404-851-7831 - Fax -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of chiggerson@memhosp.com Sent: Tuesday, August 15, 2006 12:22 PM To: Richard Yeo; Blazek, Linda Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Two patient identifiers and handwritten blocks Okay, so I do not receive any more "flaming" and vicious e-mails, let me clarify. JCAHO requires two patient identifiers when collecting specimens, administering meds, etc. JCAHO recommends and encourages laboratories to use two patient identifiers on all aliquots (this includes blocks and slides). Our institution has implemented a two patient identifier policy before taking any action with a patient, patient specimen, or patient record (NOT just specimen collection). This is institution wide. In addition, the laboratory has implemented the two patient identifier policy for all testing phases including preanalytical, analytical and postanalytical for both specimens and records. This is not only recommended by JCAHO but also recommended by CAP. Please note it is currently a recommendation, not a requirement. If you need a reference, refer to CAP's publication "Quality Management in Anatomic Pathology". Recommendations for including two patient identifiers on both frozen section slides and on all blocks and slides is located in the chapter "Strategies for Error Reduction and Prevention in Surgical Pathology". More and more institutions are taking these recommendatioins seriously and implementing policies both institution wide and laboratory wide. Mine is such a place. We have always used two patient identifiers, except on the blocks. Therefore, for those that are taking this seriously, I would like to know which two patient identifiers are used and how you implemented it. I NEED to comply with my institutional and laboratory's policies. Thanks, Cindy "Richard Yeo" 08/15/2006 10:31 AM To cc Subject RE: [Histonet] Two patient identifiers and handwritten blocks SPECIMENS COMING INTO THE LAB REQUIRE TWO PATIENT IDENTIFIERS, NAME/DATE OF BIRTH/SSN#... NOT PARAFFIN BLOCKS/SLIDES. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of chiggerson@memhosp.com Sent: Tuesday, August 15, 2006 9:37 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Two patient identifiers and handwritten blocks For those of you that are still handwriting accession numbers on your blocks: 1. Do you require both the accession number and the patient's name to be handwritten on the cassettes to meet the two patient identifier patient safety requirement? 2. If so, do you require the patient's full name? Or last name, first initial? 3. If not using the patient's name as the second identifier, what are you using? Medical Record Number? 4. If not using two patient identifiers (accession number only), how are you justifying not using two identifiers to meet the patient safety requirements? 5. For those that have implemented adding a second handwritten identifier to blocks, how did your staff react? The obvious solution is a cassette labeler, however it just is not going to happen soon. Thank you in advance for your response to this very important issue. Thanks, Cindy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From JEllin <@t> yumaregional.org Tue Aug 15 12:20:11 2006 From: JEllin <@t> yumaregional.org (Jesus Ellin) Date: Tue Aug 15 12:20:34 2006 Subject: [Histonet] Two patient identifiers and handwritten blocks Message-ID: <29BE166A2CF48D459853F8EC57CD37E83ABFCB@EXCHANGECLUSTER.yumaregional.local> I know of this one as well Victor and you are correct. But can a barcode be used as a identifier. Since it is unique to our system only, what if you send it out for a consult and the only identifier is the Accession number and barcode that is unique to what ever LIS or PIS you are using. Will this hold up on a JACHO inspection.?? This is the question at hand that only JACHO can answer I think. But since CLIA and JACHO seem to be revamping we need to look at what they are requiring inorder to see were we need to head. It seems to me that there needs to be specific clarification one what is acceptable and what isnt. We do not need to be shooting in the dark. What happens if you are not automated and do not have the capability to add 2D or 1D barcodes to the blocks. Then you are looking at the man hours that are going to be spent on just writing blocks with not only accession number but also either, MRN, SSN, or Name. We are all already having wings clipped because of budget issues and the most costly is wage. So I see some serious issues with this one. Jesus A. Ellin HT ASCP Yuma Regional Medical Center Histology Systems Technologist Pathology Information Systems 928-336-7444 or 928-336-1144 Fax: 928-336-7319 -----Original Message----- From: Victor Tobias [mailto:victor@pathology.washington.edu] Sent: Tuesday, August 15, 2006 10:09 AM To: chiggerson@memhosp.com Cc: Jesus Ellin; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Two patient identifiers and handwritten blocks In the presentation I saw for MGH, they have the accession number, block ID and barcode. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. chiggerson@memhosp.com wrote: > Good question! I think the preferred solution is a barcode that is > linked to the patient's information. I am not sure if user > readability is required. I would think not if you are using bar code > readers at embedding and microtomy like some larger institutions are > doing. I think MGH implemented such a system. They scan their block > just before microtomy and the labels for that block print right at the > microtome. You can't get better positive patient ID than that! I > assume that the accession number is also on the block because you > still have to file and retrieve it. It would be interesting to find > out if they are using just the bar code and acession number, or if they also have patient name. > > Is there any one from MGH out there that can answer this? > > Thanks, > Cindy > > > > > > "Jesus Ellin" > 08/15/2006 11:43 AM > > To > chiggerson@memhosp.com, "Richard Yeo" , "Blazek, Linda" > > cc > histonet@lists.utsouthwestern.edu > Subject > RE: [Histonet] Two patient identifiers and handwritten blocks > > > > > > > Could one of the Identifiers be a 2D barcode or just a plain barcode. > This is generted specificly for this block with this patients > information. That could be used as a identifier, or does it have to > be something that is a readable identifer to everyone that is handling > that case.?? > > > Jesus A. Ellin HT ASCP > Yuma Regional Medical Center > Histology Systems Technologist > Pathology Information Systems > 928-336-7444 or 928-336-1144 > Fax: 928-336-7319 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > chiggerson@memhosp.com > Sent: Tuesday, August 15, 2006 9:22 AM > To: Richard Yeo; Blazek, Linda > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Two patient identifiers and handwritten blocks > > Okay, so I do not receive any more "flaming" and vicious e-mails, let > me clarify. > > JCAHO requires two patient identifiers when collecting specimens, > administering meds, etc. JCAHO recommends and encourages laboratories > to use two patient identifiers on all aliquots (this includes blocks > and slides). Our institution has implemented a two patient identifier > policy before taking any action with a patient, patient specimen, or > patient record (NOT just specimen collection). This is institution > wide. In addition, the laboratory has implemented the two patient > identifier policy for all testing phases including preanalytical, > analytical and postanalytical for both specimens and records. This is > not only recommended by JCAHO but also recommended by CAP. Please note > it is currently a recommendation, not a requirement. If you need a > reference, refer to CAP's publication "Quality Management in Anatomic Pathology". > Recommendations for including two patient identifiers on both frozen > section slides and on all blocks and slides is located in the chapter > "Strategies for Error Reduction and Prevention in Surgical Pathology". > More and more institutions are taking these recommendatioins seriously > and implementing policies both institution wide and laboratory wide. > Mine is such a place. We have always used two patient identifiers, > except on the blocks. Therefore, for those that are taking this > seriously, I would like to know which two patient identifiers are used > and how you implemented it. > I NEED to comply with my institutional and laboratory's policies. > > > Thanks, > Cindy > > > > > > "Richard Yeo" > 08/15/2006 10:31 AM > > To > > cc > > Subject > RE: [Histonet] Two patient identifiers and handwritten blocks > > > > > > > SPECIMENS COMING INTO THE LAB REQUIRE TWO PATIENT IDENTIFIERS, > NAME/DATE OF BIRTH/SSN#... > NOT PARAFFIN BLOCKS/SLIDES. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > chiggerson@memhosp.com > Sent: Tuesday, August 15, 2006 9:37 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Two patient identifiers and handwritten blocks > > For those of you that are still handwriting accession numbers on your > blocks: > > 1. Do you require both the accession number and the patient's name > to > be handwritten on the cassettes to meet the two patient identifier > patient safety requirement? > > 2. If so, do you require the patient's full name? Or last name, > first > initial? > > 3. If not using the patient's name as the second identifier, what > are > you using? Medical Record Number? > > 4. If not using two patient identifiers (accession number only), > how > are you justifying not using two identifiers to meet the patient > safety requirements? > > 5. For those that have implemented adding a second handwritten > identifier to blocks, how did your staff react? > > The obvious solution is a cassette labeler, however it just is not > going to happen soon. Thank you in advance for your response to this > very important issue. > > Thanks, > Cindy > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > This message is confidential, intended only for the named > recipient(s) and may contain information that is privileged or exempt > from disclosure under applicable law. If you are not the intended > recipient(s), you are notified that the dissemination, distribution, > or copying of this message is strictly prohibited. If you receive > this message in error, or are not the named recipient(s), please > notify the sender at either the e-mail, fax, address, or telephone > number listed above and delete this e-mail from your computer. > Thank you. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. From doug <@t> ppspath.com Tue Aug 15 12:36:23 2006 From: doug <@t> ppspath.com (Douglas D Deltour) Date: Tue Aug 15 12:33:22 2006 Subject: [Histonet] Ventana Medical Systems Inc agreed to acquire Vision Systems Ltd Message-ID: FYI Ventana Medical Systems Inc agreed to acquire Vision Systems Ltd August 14, 2006 - Mt. Waverley, VI, Australia - Ventana Medical Systems Inc, Arizona-based laboratory instrument supplier, has agreed to acquire all ordinary shares in Vision Systems Ltd, a medical instrument supplier in Australia, for approximately AUD450.7 million (US$346.1 million) in cash. Under the Share Scheme, Vision Systems shareholders will receive AUD2.13 (US$1.6) per share; and Under the Notes Scheme, Vision Systems noteholders will receive AUD2.73 (US$2.1) cash for every Unsecured Notes. The acquisition is expected to accelerate both companies' strategic plans to be a diagnostic leader in the global market. The financial advisors in the deal include: Merrill Lynch & Co Inc for Ventana Medical Systems Inc, Caliburn Partnership for Vision Systems Ltd. The legal advisors in the deal include: Baker & McKenzie for Ventana Medical Systems Inc, Minter Ellison Morris Fletcher for Vision Systems Ltd. http://www.mergerstat.com/baystreet3.asp?ID=149619&s=&i= Douglas D. Deltour HT(ASCP) Histology Supervisor Professional Pathology Services, PC One Science Court Suite 200 Columbia, SC 29203 (803)252-1913 Fax (803)254-3262 ***************************************************** PROFESSIONAL PATHOLOGY SERVICES, PC NOTICE OF CONFIDENTIALITY This message is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential and exempt from disclosure under applicable law. If the reader of this message is not the intended recipient, you are hereby notified that any dissemination, distribution, or copying of this communication is strictly prohibited by law. If you have received this communication in error, please notify me immediately. From tpmorken <@t> labvision.com Tue Aug 15 12:33:43 2006 From: tpmorken <@t> labvision.com (Morken, Tim - Labvision) Date: Tue Aug 15 12:33:51 2006 Subject: [Histonet] Two patient identifiers and handwritten blocks Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA2048F7268@usca0082k08.labvision.apogent.com> I believe you need two human-readable identifiers. As some have mentioned,. The technology may not be available to read a given barcode (this mostly concerns linear "bar" codes verses 2D (grid) codes, some readers can read only the bar codes, some both). But it seems that the barcodes I've seen also have a human-readable line showing what is in the barcode. It may be small type, but it is usually there. If it is there that could be used as the second identifier. Tim -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Tuesday, August 15, 2006 10:20 AM To: Victor Tobias; chiggerson@memhosp.com Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Two patient identifiers and handwritten blocks I know of this one as well Victor and you are correct. But can a barcode be used as a identifier. Since it is unique to our system only, what if you send it out for a consult and the only identifier is the Accession number and barcode that is unique to what ever LIS or PIS you are using. Will this hold up on a JACHO inspection.?? This is the question at hand that only JACHO can answer I think. But since CLIA and JACHO seem to be revamping we need to look at what they are requiring inorder to see were we need to head. It seems to me that there needs to be specific clarification one what is acceptable and what isnt. We do not need to be shooting in the dark. What happens if you are not automated and do not have the capability to add 2D or 1D barcodes to the blocks. Then you are looking at the man hours that are going to be spent on just writing blocks with not only accession number but also either, MRN, SSN, or Name. We are all already having wings clipped because of budget issues and the most costly is wage. So I see some serious issues with this one. Jesus A. Ellin HT ASCP Yuma Regional Medical Center Histology Systems Technologist Pathology Information Systems 928-336-7444 or 928-336-1144 Fax: 928-336-7319 -----Original Message----- From: Victor Tobias [mailto:victor@pathology.washington.edu] Sent: Tuesday, August 15, 2006 10:09 AM To: chiggerson@memhosp.com Cc: Jesus Ellin; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Two patient identifiers and handwritten blocks In the presentation I saw for MGH, they have the accession number, block ID and barcode. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. chiggerson@memhosp.com wrote: > Good question! I think the preferred solution is a barcode that is > linked to the patient's information. I am not sure if user > readability is required. I would think not if you are using bar code > readers at embedding and microtomy like some larger institutions are > doing. I think MGH implemented such a system. They scan their block > just before microtomy and the labels for that block print right at the > microtome. You can't get better positive patient ID than that! I > assume that the accession number is also on the block because you > still have to file and retrieve it. It would be interesting to find > out if they are using just the bar code and acession number, or if they also have patient name. > > Is there any one from MGH out there that can answer this? > > Thanks, > Cindy > > > > > > "Jesus Ellin" > 08/15/2006 11:43 AM > > To > chiggerson@memhosp.com, "Richard Yeo" , "Blazek, Linda" > > cc > histonet@lists.utsouthwestern.edu > Subject > RE: [Histonet] Two patient identifiers and handwritten blocks > > > > > > > Could one of the Identifiers be a 2D barcode or just a plain barcode. > This is generted specificly for this block with this patients > information. That could be used as a identifier, or does it have to > be something that is a readable identifer to everyone that is handling > that case.?? > > > Jesus A. Ellin HT ASCP > Yuma Regional Medical Center > Histology Systems Technologist > Pathology Information Systems > 928-336-7444 or 928-336-1144 > Fax: 928-336-7319 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > chiggerson@memhosp.com > Sent: Tuesday, August 15, 2006 9:22 AM > To: Richard Yeo; Blazek, Linda > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Two patient identifiers and handwritten blocks > > Okay, so I do not receive any more "flaming" and vicious e-mails, let > me clarify. > > JCAHO requires two patient identifiers when collecting specimens, > administering meds, etc. JCAHO recommends and encourages laboratories > to use two patient identifiers on all aliquots (this includes blocks > and slides). Our institution has implemented a two patient identifier > policy before taking any action with a patient, patient specimen, or > patient record (NOT just specimen collection). This is institution > wide. In addition, the laboratory has implemented the two patient > identifier policy for all testing phases including preanalytical, > analytical and postanalytical for both specimens and records. This is > not only recommended by JCAHO but also recommended by CAP. Please note > it is currently a recommendation, not a requirement. If you need a > reference, refer to CAP's publication "Quality Management in Anatomic Pathology". > Recommendations for including two patient identifiers on both frozen > section slides and on all blocks and slides is located in the chapter > "Strategies for Error Reduction and Prevention in Surgical Pathology". > More and more institutions are taking these recommendatioins seriously > and implementing policies both institution wide and laboratory wide. > Mine is such a place. We have always used two patient identifiers, > except on the blocks. Therefore, for those that are taking this > seriously, I would like to know which two patient identifiers are used > and how you implemented it. > I NEED to comply with my institutional and laboratory's policies. > > > Thanks, > Cindy > > > > > > "Richard Yeo" > 08/15/2006 10:31 AM > > To > > cc > > Subject > RE: [Histonet] Two patient identifiers and handwritten blocks > > > > > > > SPECIMENS COMING INTO THE LAB REQUIRE TWO PATIENT IDENTIFIERS, > NAME/DATE OF BIRTH/SSN#... > NOT PARAFFIN BLOCKS/SLIDES. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > chiggerson@memhosp.com > Sent: Tuesday, August 15, 2006 9:37 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Two patient identifiers and handwritten blocks > > For those of you that are still handwriting accession numbers on your > blocks: > > 1. Do you require both the accession number and the patient's name > to > be handwritten on the cassettes to meet the two patient identifier > patient safety requirement? > > 2. If so, do you require the patient's full name? Or last name, > first > initial? > > 3. If not using the patient's name as the second identifier, what > are > you using? Medical Record Number? > > 4. If not using two patient identifiers (accession number only), > how > are you justifying not using two identifiers to meet the patient > safety requirements? > > 5. For those that have implemented adding a second handwritten > identifier to blocks, how did your staff react? > > The obvious solution is a cassette labeler, however it just is not > going to happen soon. Thank you in advance for your response to this > very important issue. > > Thanks, > Cindy > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > This message is confidential, intended only for the named > recipient(s) and may contain information that is privileged or exempt > from disclosure under applicable law. If you are not the intended > recipient(s), you are notified that the dissemination, distribution, > or copying of this message is strictly prohibited. If you receive > this message in error, or are not the named recipient(s), please > notify the sender at either the e-mail, fax, address, or telephone > number listed above and delete this e-mail from your computer. > Thank you. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kimtournear <@t> yahoo.com Tue Aug 15 12:47:04 2006 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Tue Aug 15 12:47:11 2006 Subject: [Histonet] SurgiPath Paraffin Message-ID: <20060815174704.72688.qmail@web37714.mail.mud.yahoo.com> Hi all, I use the SurgiPath infiltration paraffin and the embedding paraffin in my lab....I've noticed a lot of "grit" in my paraffin chamber (embedding station) as well as the stations on the tx processor. It's not lot# specific....I'm changing all paraffin stations on the processor every Friday as well as emptying out the embedding chamber (cleaning with xylene and 100% alcohol, as well as the filter) and by Monday, the grit is there again. Anyone out there having the same problem???Any help would be appreciated... Kim Tournear, HT (ASCP), QIHC (ASCP) Specialists in Dermatology Tucson, AZ --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. From tonia.richmond <@t> thermo.com Tue Aug 15 12:54:49 2006 From: tonia.richmond <@t> thermo.com (Richmond, Tonia) Date: Tue Aug 15 12:54:59 2006 Subject: [Histonet] Two patient identifiers and handwritten blocks Message-ID: If you don't mind me putting my two-cents worth in I'd like to say that Thermo Shandon has a system that will print barcodes on cassettes and slides. The cassettes can be scanned at the microtome station and the "printed" slide (not a label) will print as the technician is sectioning, right there at the microtome. How convenient is that?! The barcodes can be 1-D or 2-D. 2-D is more poplar because you can put information like the patient name in the barcode allowing room on the cassette/slide for the accession number, etc. Best Regards, Tonia J. Richmond, HT (ASCP) Cell: (870) 575-3307 Fax: (870) 879-9639 Email: tonia.richmond@thermo.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Morken, Tim - Labvision Sent: Tuesday, August 15, 2006 12:34 PM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Two patient identifiers and handwritten blocks I believe you need two human-readable identifiers. As some have mentioned,. The technology may not be available to read a given barcode (this mostly concerns linear "bar" codes verses 2D (grid) codes, some readers can read only the bar codes, some both). But it seems that the barcodes I've seen also have a human-readable line showing what is in the barcode. It may be small type, but it is usually there. If it is there that could be used as the second identifier. Tim -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jesus Ellin Sent: Tuesday, August 15, 2006 10:20 AM To: Victor Tobias; chiggerson@memhosp.com Cc: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Two patient identifiers and handwritten blocks I know of this one as well Victor and you are correct. But can a barcode be used as a identifier. Since it is unique to our system only, what if you send it out for a consult and the only identifier is the Accession number and barcode that is unique to what ever LIS or PIS you are using. Will this hold up on a JACHO inspection.?? This is the question at hand that only JACHO can answer I think. But since CLIA and JACHO seem to be revamping we need to look at what they are requiring inorder to see were we need to head. It seems to me that there needs to be specific clarification one what is acceptable and what isnt. We do not need to be shooting in the dark. What happens if you are not automated and do not have the capability to add 2D or 1D barcodes to the blocks. Then you are looking at the man hours that are going to be spent on just writing blocks with not only accession number but also either, MRN, SSN, or Name. We are all already having wings clipped because of budget issues and the most costly is wage. So I see some serious issues with this one. Jesus A. Ellin HT ASCP Yuma Regional Medical Center Histology Systems Technologist Pathology Information Systems 928-336-7444 or 928-336-1144 Fax: 928-336-7319 -----Original Message----- From: Victor Tobias [mailto:victor@pathology.washington.edu] Sent: Tuesday, August 15, 2006 10:09 AM To: chiggerson@memhosp.com Cc: Jesus Ellin; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Two patient identifiers and handwritten blocks In the presentation I saw for MGH, they have the accession number, block ID and barcode. Victor Victor Tobias Clinical Applications Analyst University of Washington Medical Center Dept of Pathology Room BB220 1959 NE Pacific Seattle, WA 98195 victor@pathology.washington.edu 206-598-2792 206-598-7659 Fax ================================================= Privileged, confidential or patient identifiable information may be contained in this message. This information is meant only for the use of the intended recipients. If you are not the intended recipient, or if the message has been addressed to you in error, do not read, disclose, reproduce, distribute, disseminate or otherwise use this transmission. Instead, please notify the sender by reply e-mail, and then destroy all copies of the message and any attachments. chiggerson@memhosp.com wrote: > Good question! I think the preferred solution is a barcode that is > linked to the patient's information. I am not sure if user > readability is required. I would think not if you are using bar code > readers at embedding and microtomy like some larger institutions are > doing. I think MGH implemented such a system. They scan their block > just before microtomy and the labels for that block print right at the > microtome. You can't get better positive patient ID than that! I > assume that the accession number is also on the block because you > still have to file and retrieve it. It would be interesting to find > out if they are using just the bar code and acession number, or if they also have patient name. > > Is there any one from MGH out there that can answer this? > > Thanks, > Cindy > > > > > > "Jesus Ellin" > 08/15/2006 11:43 AM > > To > chiggerson@memhosp.com, "Richard Yeo" , "Blazek, Linda" > > cc > histonet@lists.utsouthwestern.edu > Subject > RE: [Histonet] Two patient identifiers and handwritten blocks > > > > > > > Could one of the Identifiers be a 2D barcode or just a plain barcode. > This is generted specificly for this block with this patients > information. That could be used as a identifier, or does it have to > be something that is a readable identifer to everyone that is handling > that case.?? > > > Jesus A. Ellin HT ASCP > Yuma Regional Medical Center > Histology Systems Technologist > Pathology Information Systems > 928-336-7444 or 928-336-1144 > Fax: 928-336-7319 > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > chiggerson@memhosp.com > Sent: Tuesday, August 15, 2006 9:22 AM > To: Richard Yeo; Blazek, Linda > Cc: histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Two patient identifiers and handwritten blocks > > Okay, so I do not receive any more "flaming" and vicious e-mails, let > me clarify. > > JCAHO requires two patient identifiers when collecting specimens, > administering meds, etc. JCAHO recommends and encourages laboratories > to use two patient identifiers on all aliquots (this includes blocks > and slides). Our institution has implemented a two patient identifier > policy before taking any action with a patient, patient specimen, or > patient record (NOT just specimen collection). This is institution > wide. In addition, the laboratory has implemented the two patient > identifier policy for all testing phases including preanalytical, > analytical and postanalytical for both specimens and records. This is > not only recommended by JCAHO but also recommended by CAP. Please note > it is currently a recommendation, not a requirement. If you need a > reference, refer to CAP's publication "Quality Management in Anatomic Pathology". > Recommendations for including two patient identifiers on both frozen > section slides and on all blocks and slides is located in the chapter > "Strategies for Error Reduction and Prevention in Surgical Pathology". > More and more institutions are taking these recommendatioins seriously > and implementing policies both institution wide and laboratory wide. > Mine is such a place. We have always used two patient identifiers, > except on the blocks. Therefore, for those that are taking this > seriously, I would like to know which two patient identifiers are used > and how you implemented it. > I NEED to comply with my institutional and laboratory's policies. > > > Thanks, > Cindy > > > > > > "Richard Yeo" > 08/15/2006 10:31 AM > > To > > cc > > Subject > RE: [Histonet] Two patient identifiers and handwritten blocks > > > > > > > SPECIMENS COMING INTO THE LAB REQUIRE TWO PATIENT IDENTIFIERS, > NAME/DATE OF BIRTH/SSN#... > NOT PARAFFIN BLOCKS/SLIDES. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of > chiggerson@memhosp.com > Sent: Tuesday, August 15, 2006 9:37 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Two patient identifiers and handwritten blocks > > For those of you that are still handwriting accession numbers on your > blocks: > > 1. Do you require both the accession number and the patient's name > to > be handwritten on the cassettes to meet the two patient identifier > patient safety requirement? > > 2. If so, do you require the patient's full name? Or last name, > first > initial? > > 3. If not using the patient's name as the second identifier, what > are > you using? Medical Record Number? > > 4. If not using two patient identifiers (accession number only), > how > are you justifying not using two identifiers to meet the patient > safety requirements? > > 5. For those that have implemented adding a second handwritten > identifier to blocks, how did your staff react? > > The obvious solution is a cassette labeler, however it just is not > going to happen soon. Thank you in advance for your response to this > very important issue. > > Thanks, > Cindy > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > This message is confidential, intended only for the named > recipient(s) and may contain information that is privileged or exempt > from disclosure under applicable law. If you are not the intended > recipient(s), you are notified that the dissemination, distribution, > or copying of this message is strictly prohibited. If you receive > this message in error, or are not the named recipient(s), please > notify the sender at either the e-mail, fax, address, or telephone > number listed above and delete this e-mail from your computer. > Thank you. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Jackie.O'Connor <@t> abbott.com Tue Aug 15 13:22:59 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Tue Aug 15 13:23:31 2006 Subject: [Histonet] SurgiPath Paraffin In-Reply-To: <20060815174704.72688.qmail@web37714.mail.mud.yahoo.com> Message-ID: In the paraffin production process, some dust and a bit o' dirt can get into the process - it's harmless. I know if you call Ken Urban at Surgipath Industries in Richmond, Illinois - he will ease your mind. He's a genius. Jackie O' Kim Tournear Sent by: histonet-bounces@lists.utsouthwestern.edu 08/15/2006 12:47 PM To Histonet cc Subject [Histonet] SurgiPath Paraffin Hi all, I use the SurgiPath infiltration paraffin and the embedding paraffin in my lab....I've noticed a lot of "grit" in my paraffin chamber (embedding station) as well as the stations on the tx processor. It's not lot# specific....I'm changing all paraffin stations on the processor every Friday as well as emptying out the embedding chamber (cleaning with xylene and 100% alcohol, as well as the filter) and by Monday, the grit is there again. Anyone out there having the same problem???Any help would be appreciated... Kim Tournear, HT (ASCP), QIHC (ASCP) Specialists in Dermatology Tucson, AZ --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From gcallis <@t> montana.edu Tue Aug 15 13:49:57 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Aug 15 13:50:04 2006 Subject: [Histonet] SurgiPath Paraffin In-Reply-To: <20060815174704.72688.qmail@web37714.mail.mud.yahoo.com> References: <20060815174704.72688.qmail@web37714.mail.mud.yahoo.com> Message-ID: <6.0.0.22.1.20060815124534.01b52c50@gemini.msu.montana.edu> Not sure what you mean by grit? Do you stir your paraffin BEFORE embedding? You may have the plastic polymers settling out to bottom of container. if you pour pre melted paraffin into your processor paraffin containers, give it a stir too. Redistribution of polymers ensures the proper mixture for good sectioning. We used this excellent paraffin system (infiltration media then embedding medias) for years without any problems. At 11:47 AM 8/15/2006, you wrote: >Hi all, > I use the SurgiPath infiltration paraffin and the embedding paraffin in > my lab....I've noticed a lot of "grit" in my paraffin chamber (embedding > station) as well as the stations on the tx processor. It's not lot# > specific....I'm changing all paraffin stations on the processor every > Friday as well as emptying out the embedding chamber (cleaning with > xylene and 100% alcohol, as well as the filter) and by Monday, the grit > is there again. Anyone out there having the same problem???Any help would > be appreciated... > > >Kim Tournear, HT (ASCP), QIHC (ASCP) >Specialists in Dermatology >Tucson, AZ > >--------------------------------- >Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great >rates starting at 1?/min. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From JMahoney <@t> alegent.org Tue Aug 15 14:04:12 2006 From: JMahoney <@t> alegent.org (Janice Mahoney) Date: Tue Aug 15 14:07:38 2006 Subject: [Histonet] SurgiPath Paraffin Message-ID: Now I've heard Ken called many things but this is the first time I've heard "genius" (grin). Jan and Louanne Omaha >>> "Jackie M O'Connor" 08/15/2006 1:22 PM >>> In the paraffin production process, some dust and a bit o' dirt can get into the process - it's harmless. I know if you call Ken Urban at Surgipath Industries in Richmond, Illinois - he will ease your mind. He's a genius. Jackie O' Kim Tournear Sent by: histonet-bounces@lists.utsouthwestern.edu 08/15/2006 12:47 PM To Histonet cc Subject [Histonet] SurgiPath Paraffin Hi all, I use the SurgiPath infiltration paraffin and the embedding paraffin in my lab....I've noticed a lot of "grit" in my paraffin chamber (embedding station) as well as the stations on the tx processor. It's not lot# specific....I'm changing all paraffin stations on the processor every Friday as well as emptying out the embedding chamber (cleaning with xylene and 100% alcohol, as well as the filter) and by Monday, the grit is there again. Anyone out there having the same problem???Any help would be appreciated... Kim Tournear, HT (ASCP), QIHC (ASCP) Specialists in Dermatology Tucson, AZ --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JWEEMS <@t> sjha.org Tue Aug 15 14:14:37 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Aug 15 14:14:57 2006 Subject: [Histonet] SurgiPath Paraffin Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEE669@sjhaexc02.sjha.org> And all these years, we just thought he was Ken! There's a first time for everything!!! j:>) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Janice Mahoney Sent: Tuesday, August 15, 2006 3:04 PM To: Jackie.O'Connor@abbott.com; histonet-bounces@lists.utsouthwestern.edu; kimtournear@yahoo.com Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] SurgiPath Paraffin Now I've heard Ken called many things but this is the first time I've heard "genius" (grin). Jan and Louanne Omaha >>> "Jackie M O'Connor" 08/15/2006 1:22 PM >>> In the paraffin production process, some dust and a bit o' dirt can get into the process - it's harmless. I know if you call Ken Urban at Surgipath Industries in Richmond, Illinois - he will ease your mind. He's a genius. Jackie O' Kim Tournear Sent by: histonet-bounces@lists.utsouthwestern.edu 08/15/2006 12:47 PM To Histonet cc Subject [Histonet] SurgiPath Paraffin Hi all, I use the SurgiPath infiltration paraffin and the embedding paraffin in my lab....I've noticed a lot of "grit" in my paraffin chamber (embedding station) as well as the stations on the tx processor. It's not lot# specific....I'm changing all paraffin stations on the processor every Friday as well as emptying out the embedding chamber (cleaning with xylene and 100% alcohol, as well as the filter) and by Monday, the grit is there again. Anyone out there having the same problem???Any help would be appreciated... Kim Tournear, HT (ASCP), QIHC (ASCP) Specialists in Dermatology Tucson, AZ --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From lelpers <@t> bioanalytical.com Tue Aug 15 14:26:58 2006 From: lelpers <@t> bioanalytical.com (LaDonna G. Elpers) Date: Tue Aug 15 14:27:37 2006 Subject: [Histonet] SurgiPath Paraffin Message-ID: <3100BDBD96C0724D9CE9E476BFB57D962DD071@basi10003.BIOANALYTICAL.COM> Have the same issues with paraplast that I purchase from Fisher. It is a settlement (stays on the bottom/floor of the units) therefore I don't have any issues with it getting into my product. LaDonna -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Tuesday, August 15, 2006 1:23 PM To: Kim Tournear Cc: Histonet; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] SurgiPath Paraffin In the paraffin production process, some dust and a bit o' dirt can get into the process - it's harmless. I know if you call Ken Urban at Surgipath Industries in Richmond, Illinois - he will ease your mind. He's a genius. Jackie O' Kim Tournear Sent by: histonet-bounces@lists.utsouthwestern.edu 08/15/2006 12:47 PM To Histonet cc Subject [Histonet] SurgiPath Paraffin Hi all, I use the SurgiPath infiltration paraffin and the embedding paraffin in my lab....I've noticed a lot of "grit" in my paraffin chamber (embedding station) as well as the stations on the tx processor. It's not lot# specific....I'm changing all paraffin stations on the processor every Friday as well as emptying out the embedding chamber (cleaning with xylene and 100% alcohol, as well as the filter) and by Monday, the grit is there again. Anyone out there having the same problem???Any help would be appreciated... Kim Tournear, HT (ASCP), QIHC (ASCP) Specialists in Dermatology Tucson, AZ --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMahoney <@t> alegent.org Tue Aug 15 14:31:50 2006 From: JMahoney <@t> alegent.org (Janice Mahoney) Date: Tue Aug 15 14:36:13 2006 Subject: Fwd: Re: [Histonet] SurgiPath Paraffin Message-ID: >>> Janice Mahoney 08/15/2006 2:13 PM >>> This is just a joke, Ken is great. We have known him for 30+ years. >>> Kim Tournear 08/15/2006 2:06 PM >>> I don't know this Ken...but it doesn't sound to me like he's winning any popularity contests.... Janice Mahoney wrote: Now I've heard Ken called many things but this is the first time I've heard "genius" (grin). Jan and Louanne Omaha >>> "Jackie M O'Connor" 08/15/2006 1:22 PM >>> In the paraffin production process, some dust and a bit o' dirt can get into the process - it's harmless. I know if you call Ken Urban at Surgipath Industries in Richmond, Illinois - he will ease your mind. He's a genius. Jackie O' Kim Tournear Sent by: histonet-bounces@lists.utsouthwestern.edu 08/15/2006 12:47 PM To Histonet cc Subject [Histonet] SurgiPath Paraffin Hi all, I use the SurgiPath infiltration paraffin and the embedding paraffin in my lab....I've noticed a lot of "grit" in my paraffin chamber (embedding station) as well as the stations on the tx processor. It's not lot# specific....I'm changing all paraffin stations on the processor every Friday as well as emptying out the embedding chamber (cleaning with xylene and 100% alcohol, as well as the filter) and by Monday, the grit is there again. Anyone out there having the same problem???Any help would be appreciated... Kim Tournear, HT (ASCP), QIHC (ASCP) Specialists in Dermatology Tucson, AZ --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Kim Tournear, HT (ASCP), QIHC (ASCP) Specialists in Dermatology Tucson, AZ --------------------------------- Stay in the know. Pulse on the new Yahoo.com. Check it out. From jstaruk <@t> masshistology.com Tue Aug 15 14:58:58 2006 From: jstaruk <@t> masshistology.com (Jim Staruk) Date: Tue Aug 15 14:59:23 2006 Subject: [Histonet] SurgiPath Paraffin In-Reply-To: <3100BDBD96C0724D9CE9E476BFB57D962DD071@basi10003.BIOANALYTICAL.COM> Message-ID: <000001c6c0a5$3f83b1d0$6500a8c0@FrontOffice> I was also concerned about this sediment. Using a disposable pipette, I made a blank block just from this "crud", sectioned it and H&E stained it. The slide was absolutely clear of any foreign bodies, amorphous material, pathogens or anything. I never thought of it again (although we do clean out our paraffin baths once a month so this stuff doesn't build up). Jim ______________________ Jim Staruk Mass Histology Service www.masshistology.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LaDonna G. Elpers Sent: Tuesday, August 15, 2006 3:27 PM To: Jackie M O'Connor; Kim Tournear Cc: Histonet; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] SurgiPath Paraffin Have the same issues with paraplast that I purchase from Fisher. It is a settlement (stays on the bottom/floor of the units) therefore I don't have any issues with it getting into my product. LaDonna -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Tuesday, August 15, 2006 1:23 PM To: Kim Tournear Cc: Histonet; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] SurgiPath Paraffin In the paraffin production process, some dust and a bit o' dirt can get into the process - it's harmless. I know if you call Ken Urban at Surgipath Industries in Richmond, Illinois - he will ease your mind. He's a genius. Jackie O' Kim Tournear Sent by: histonet-bounces@lists.utsouthwestern.edu 08/15/2006 12:47 PM To Histonet cc Subject [Histonet] SurgiPath Paraffin Hi all, I use the SurgiPath infiltration paraffin and the embedding paraffin in my lab....I've noticed a lot of "grit" in my paraffin chamber (embedding station) as well as the stations on the tx processor. It's not lot# specific....I'm changing all paraffin stations on the processor every Friday as well as emptying out the embedding chamber (cleaning with xylene and 100% alcohol, as well as the filter) and by Monday, the grit is there again. Anyone out there having the same problem???Any help would be appreciated... Kim Tournear, HT (ASCP), QIHC (ASCP) Specialists in Dermatology Tucson, AZ --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kallred <@t> bellsouth.net Tue Aug 15 15:29:56 2006 From: kallred <@t> bellsouth.net (Kim Allred) Date: Tue Aug 15 15:25:23 2006 Subject: [Histonet] re: Surgipath parrafin Message-ID: <20060815202514.HOJR8982.ibm63aec.bellsouth.net@D6GV9G51> I have used different paraffins for a long time. They all have a sediment in the bottom of the embedding centers. There are plenty more things in the lab to be concerned with besides this trivial thing. From gcallis <@t> montana.edu Tue Aug 15 15:35:07 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Aug 15 15:35:14 2006 Subject: [Histonet] Volunteers needed for VIR committee table at NSH/Phoenix S/C Message-ID: <6.0.0.22.1.20060815142657.01b506e0@gemini.msu.montana.edu> Dear Histonetters who are NSH-VIR committee members attending NSH S/C Phoenix AZ On behalf of the Veterinary, Industry and Research Chair - Diane Sterchi is asking for volunteers to sit at the VIR table during coffee breaks and noon hours on Sat, Sun, Mon and part of Tuesday (tear down day). This is always a fun time, meeting new friends, visiting with old friends while helping the VIR distribute information about the committee and promote NSH. Join us in Phoenix! Please contact Diane at d.sterchi@lilly.com for a time you would like, she has the sign up list. Thanks in advance Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From ROrr <@t> enh.org Tue Aug 15 15:37:23 2006 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Tue Aug 15 15:37:31 2006 Subject: [Histonet] Accu Edge Blades give away Message-ID: Hi Kids, I am in the process of cleaning the lab. I found two boxes of unopened Accu Edge LOW PROFILE disposable blades. They've been sitting around here for about 2 years. Anyone interested? First come first serve. Just send me fed ex or UPS account number and I'll ship them to you with all the histo love in my heart. Becky Orr, CLA, HT(ASCP) QIHC Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2763 From bills <@t> icpmr.wsahs.nsw.gov.au Tue Aug 15 16:16:33 2006 From: bills <@t> icpmr.wsahs.nsw.gov.au (Bill Sinai) Date: Tue Aug 15 16:17:03 2006 Subject: [Histonet] SurgiPath Paraffin In-Reply-To: <3100BDBD96C0724D9CE9E476BFB57D962DD071@wsahs.nsw.gov.au> Message-ID: <002b01c6c0b0$15862dd0$41ce080a@wsahs.nsw.gov.au> Dear All, Many years ago a fellow in Australia was manufacturing wax for histology and I was assisting him during testing. He assured me that it was perfectly normal for any wax stored for long periods to have some "crud" at the bottom of the container and that if you stirred the container it would go away. Apparently it has something to do with some of the wax being slightly denser and separates out from the other. All the best Bill Sinai Chief Hospital Scientist Tissue Pathology ICPMR Westmead Hospital Westmead NSW 2145 Phone 02 9845 7774 Fax 02 9687 2330 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LaDonna G. Elpers Sent: Wednesday, 16 August 2006 5:27 AM To: Jackie M O'Connor; Kim Tournear Cc: Histonet; histonet-bounces@lists.utsouthwestern.edu Subject: RE: [Histonet] SurgiPath Paraffin Have the same issues with paraplast that I purchase from Fisher. It is a settlement (stays on the bottom/floor of the units) therefore I don't have any issues with it getting into my product. LaDonna -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jackie M O'Connor Sent: Tuesday, August 15, 2006 1:23 PM To: Kim Tournear Cc: Histonet; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] SurgiPath Paraffin In the paraffin production process, some dust and a bit o' dirt can get into the process - it's harmless. I know if you call Ken Urban at Surgipath Industries in Richmond, Illinois - he will ease your mind. He's a genius. Jackie O' Kim Tournear Sent by: histonet-bounces@lists.utsouthwestern.edu 08/15/2006 12:47 PM To Histonet cc Subject [Histonet] SurgiPath Paraffin Hi all, I use the SurgiPath infiltration paraffin and the embedding paraffin in my lab....I've noticed a lot of "grit" in my paraffin chamber (embedding station) as well as the stations on the tx processor. It's not lot# specific....I'm changing all paraffin stations on the processor every Friday as well as emptying out the embedding chamber (cleaning with xylene and 100% alcohol, as well as the filter) and by Monday, the grit is there again. Anyone out there having the same problem???Any help would be appreciated... Kim Tournear, HT (ASCP), QIHC (ASCP) Specialists in Dermatology Tucson, AZ --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________________________________________________________________ This electronic message and any attachments may be confidential. If you are not the intended recipient of this message would you please delete the message and any attachments and advise the sender. Sydney West Area Health Service (SWAHS) uses virus scanning software but excludes any liability for viruses contained in any email or attachment. This email may contain privileged and confidential information intended only for the use of the addressees named above. If you are not the intended recipient of this email, you are hereby notified that any use, dissemination, distribution, or reproduction of this email is prohibited. If you have received this email in error, please notify SWAHS immediately. Any views expressed in this email are those of the individual sender except where the sender expressly and with authority states them to be the views of SWAHS. From kimtournear <@t> yahoo.com Tue Aug 15 18:36:17 2006 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Tue Aug 15 18:36:25 2006 Subject: [Histonet] SurgiPath Paraffin Message-ID: <20060815233617.99050.qmail@web37713.mail.mud.yahoo.com> I want to thank everyone for their input regarding my paraffin issue....it looks like I might have a dusty lab....that happens in Arizona I guess...all the sand....thanks again... Kim Tournear, HT (ASCP), QIHC (ASCP) Specialists in Dermatology Tucson, AZ --------------------------------- Get your email and more, right on the new Yahoo.com From lanbergld <@t> vcu.edu Tue Aug 15 20:03:25 2006 From: lanbergld <@t> vcu.edu (Lawrence D Lanberg/O/VCU) Date: Tue Aug 15 20:03:32 2006 Subject: [Histonet] Question about molecular sieves for EtOh Message-ID: Dear Histonet Community, Today I added 3A bead molecular sieves to my EtOh, so I can have (hopefully) a better tissue dehydration tomorrow. But the ethanol took on quite a murky appearance. It seemed that after a few hours it cleared, just a tiny bit. But it still looks kind of scary. Does this go away completely -- with longer sitting of the ethanol??? Or does anybody know a good way to separate, without the chance of humidity getting back into the EtOh? Thanks so much. Larry Lanberg From josephnerk <@t> hotmail.com Tue Aug 15 21:42:19 2006 From: josephnerk <@t> hotmail.com (Joseph Nerk) Date: Tue Aug 15 21:42:27 2006 Subject: [Histonet] Immunostaining for H.pylori Message-ID: Does any in histo land can recommend a good Immunostaining detection kit for Helicobacter pylori, have been seeing a lit recently but the pathologist looks at the Giemsa but still will like the Immunostaining method. Awaiting your response. _________________________________________________________________ Express yourself instantly with MSN Messenger! [1]MSN Messenger Download today it's FREE! References 1. http://g.msn.com/8HMBEN/2740??PS=47575 From RCHIOVETTI <@t> aol.com Wed Aug 16 00:44:47 2006 From: RCHIOVETTI <@t> aol.com (RCHIOVETTI@aol.com) Date: Wed Aug 16 00:45:04 2006 Subject: [Histonet] Question about molecular sieves for EtOh Message-ID: <54d.5e7ec78.32140acf@aol.com> In a message dated 8/15/2006 6:04:23 PM US Mountain Standard Time, lanbergld@vcu.edu writes: > Today I added 3A bead molecular sieves to my EtOh, so I can have > (hopefully) a better tissue dehydration tomorrow. But the ethanol took on > quite a murky appearance. It seemed that after a few hours it cleared, just > a tiny bit. But it still looks kind of scary. > > Does this go away completely -- with longer sitting of the ethanol??? Or > does anybody know a good way to separate, without the chance of humidity > getting back into the EtOh? > > Thanks so much. > Larry, I've used 5A molecular sieve in ETOH for the same purpose, and I got exactly the same result: cloudy ETOH. The cloudiness does go away after a couple of days and the sediment will collect on the bottom of the bottle. I think it must be the result of small pieces of molecular sieve coming off the pellets. I always played it safe...letting the ETOH stand for a couple of days, then carefully removing or decanting from the top to use it. I also tried filtering the ETOH in a syringe with a luer-lok Millipore filter (0.4 micron pores, if I remember correctly) attached to the syringe, but honestly I could see no difference between decanting and filtering. Both kinds of ETOH worked fine, and I never saw any kind of "crud" in my specimens. I used the ETOH for electron microscopy specimen prep, so you'd think the precipitate would certainly be visible if it was present. But I never had any problems. I also used this technique to keep acetone anhydrous. It worked like a charm! Hope this helps. Cheers, Bob Robert (Bob) Chiovetti, Ph.D. Owner The Microscope Works Arizona's Microscopy Resource 132 North Elster Drive Tucson, AZ 85710-3212 USA Tel./Fax 520-546-4986 Manufacturers' Representative Member, Arizona Small Business Association - ASBA From sbreeden <@t> nmda.nmsu.edu Wed Aug 16 07:02:07 2006 From: sbreeden <@t> nmda.nmsu.edu (Breeden, Sara) Date: Wed Aug 16 07:02:12 2006 Subject: [Histonet] Histonet Gathering at NSH? Message-ID: Will there be a gathering of Histonetters at NSH this year? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 700 Albuquerque, NM 87106 505-841-2576 From rjbuesa <@t> yahoo.com Wed Aug 16 07:26:24 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Aug 16 07:26:30 2006 Subject: [Histonet] Question about molecular sieves for EtOh In-Reply-To: Message-ID: <20060816122624.44575.qmail@web61213.mail.yahoo.com> Lawrence: If you want to have really anhydrous EtOH, why don't you just add an excess of anhydrous copper sulfate (white powder) that will absorb any water in the EtOH? I used this method for years and changed the powder when it began to turn blue. Copper sulfate anh. will not only dehydrate the EtOH but will indicate you also when to change it and probably will be cheaper that your present method. Hope this will help you! Ren? J. Lawrence D Lanberg/O/VCU wrote: Dear Histonet Community, Today I added 3A bead molecular sieves to my EtOh, so I can have (hopefully) a better tissue dehydration tomorrow. But the ethanol took on quite a murky appearance. It seemed that after a few hours it cleared, just a tiny bit. But it still looks kind of scary. Does this go away completely -- with longer sitting of the ethanol??? Or does anybody know a good way to separate, without the chance of humidity getting back into the EtOh? Thanks so much. Larry Lanberg _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Stay in the know. Pulse on the new Yahoo.com. Check it out. From oshel1pe <@t> cmich.edu Wed Aug 16 07:31:51 2006 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Wed Aug 16 07:32:08 2006 Subject: [Histonet] Question about molecular sieves for EtOh In-Reply-To: References: Message-ID: Larry, The murkiness is fine dust from the molecular sieve. It settles out after a few to several hours. You have to remember to never shake the bottle or pour from it. Use a transfer pipette to get EtOH out of the bottle. Or: Put the sieve in dialysis tubing. This will contain the dust, but do as effect a job of keeping the EtOH dry. Sieve works very well. I've used it for years. Phil >Dear Histonet Community, > >Today I added 3A bead molecular sieves to my EtOh, so I can have >(hopefully) a better tissue dehydration tomorrow. But the ethanol took on >quite a murky appearance. It seemed that after a few hours it cleared, just >a tiny bit. But it still looks kind of scary. > >Does this go away completely -- with longer sitting of the ethanol??? Or >does anybody know a good way to separate, without the chance of humidity >getting back into the EtOh? > >Thanks so much. > >Larry Lanberg From oshel1pe <@t> cmich.edu Wed Aug 16 07:38:51 2006 From: oshel1pe <@t> cmich.edu (Philip Oshel) Date: Wed Aug 16 07:39:00 2006 Subject: [Histonet] Question about molecular sieves for EtOh In-Reply-To: <20060816122624.44575.qmail@web61213.mail.yahoo.com> References: <20060816122624.44575.qmail@web61213.mail.yahoo.com> Message-ID: Ren?, Anhydrous copper sulfate works, but it has a worse dust problem than does molecular sieve. Also, molecular sieve can hold 3 to 4 times more water (on a weight basis) than can copper sulfate. Copper sulfate is cheaper, but in the long run, I think sieve is cheaper. Buy 5 pound cans from e.g. Fisher. Note: don't buy either of these with indicator! The indicator dye dissolves in the alcohol. Both can be regenerated (dried) in a 200 deg C oven for 3 hours or so, then re-used. Phil >Lawrence: > If you want to have really anhydrous EtOH, why >don't you just add an excess of anhydrous copper >sulfate (white powder) that will absorb any >water in the EtOH? > I used this method for years and changed the >powder when it began to turn blue. > Copper sulfate anh. will not only dehydrate >the EtOH but will indicate you also when to >change it and probably will be cheaper that your >present method. > Hope this will help you! > Ren? J. > >Lawrence D Lanberg/O/VCU wrote: > >Dear Histonet Community, > >Today I added 3A bead molecular sieves to my EtOh, so I can have >(hopefully) a better tissue dehydration tomorrow. But the ethanol took on >quite a murky appearance. It seemed that after a few hours it cleared, just >a tiny bit. But it still looks kind of scary. > >Does this go away completely -- with longer sitting of the ethanol??? Or >does anybody know a good way to separate, without the chance of humidity >getting back into the EtOh? > >Thanks so much. > >Larry Lanberg -- Philip Oshel Microscopy Facility Supervisor Department of Biology Central Michigan University 024C Brooks Hall Mt. Pleasant, MI 48859 voice: (989) 774-3576 dept. fax: (989) 774-3462 From christahercher <@t> yahoo.ca Wed Aug 16 08:17:48 2006 From: christahercher <@t> yahoo.ca (christa hercher) Date: Wed Aug 16 08:17:56 2006 Subject: [Histonet] marking a tissue block Message-ID: <20060816131748.8690.qmail@web56515.mail.re3.yahoo.com> Hello Histonets, I was wondering if anyone has suggestions on how to mark a block of tissue in order to orient yourself when looking at the sections under the microscope. The mark will have to go through the entire block of tissue. The tissue is also being stained with golgi so the mark has to be something that will not react with the stain. Ideas and comments would be greatly appreciated. smiles, Christa Hercher McGill University "Don't blame it on the sunshine, don't blame it on the moonlight, don't blame it on good times, blame it on the boogie." Captain Jack --------------------------------- Be smarter than spam. See how smart SpamGuard is at giving junk email the boot with the All-new Yahoo! Mail From Charlene.Henry <@t> STJUDE.ORG Wed Aug 16 09:47:47 2006 From: Charlene.Henry <@t> STJUDE.ORG (Henry, Charlene) Date: Wed Aug 16 09:47:54 2006 Subject: [Histonet] (no subject) Message-ID: <5CB39BCA5724F349BCB748675C6CA1A2099A1D92@SJMEMXMB02.stjude.sjcrh.local> Good morning to everyone. Does anyone know of a CD133 antibody that works with FFPE tissues? Thanks, Charlene From ree3 <@t> leicester.ac.uk Wed Aug 16 10:43:03 2006 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Wed Aug 16 10:43:11 2006 Subject: [Histonet] Choline kinase In-Reply-To: Message-ID: Anybody aware of an antibody to choline kinase that works on paraffin processed mouse tissues??many thanks. Richard Edwards MRC TOXICOLOGY UNIT LEICESTER..U.K.... _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pex0220 <@t> yahoo.com.cn Wed Aug 16 10:48:16 2006 From: pex0220 <@t> yahoo.com.cn (pex) Date: Wed Aug 16 10:48:26 2006 Subject: [Histonet] Kluever Barrera staining Message-ID: <20060816154816.75860.qmail@web15202.mail.cnb.yahoo.com> Hello, everybody, Can you provide some information about Kluever Barrera staining? Because I find the staining of skeleton in mouse embryo is very strong with Kluever Barrera staining. Greetings Guofeng --------------------------------- Mp3·čżńËŃ-иčČȸč¸ßËŮĎ From BGuidos <@t> lab.uropartners.com Wed Aug 16 11:41:06 2006 From: BGuidos <@t> lab.uropartners.com (Barbara Guidos) Date: Wed Aug 16 11:41:24 2006 Subject: [Histonet] Question about purchasing refurbished equipment Message-ID: <5DA1CA5D0B98A84985B545A24423B822021DDD@UPLAB01.uplab.local> Hello All - I am looking to purchase a cytology/ histology slide stainer and would be interested in knowing if you have any experience (positive or negative) in purchasing refurbished laboratory equipment through biomedical corporations such as Rankin, LabX, etc. Feel free to send your comments to my email address below. Thanks for your help. Barb Guidos __________________________________ Barbara J. Guidos, SCT(ASCP), CT(IAC) UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL. 60154 (T) 708.486.0076 (F) 708.486.0080 bguidos@lab.uropartners.com From Rcartun <@t> harthosp.org Wed Aug 16 11:44:21 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Wed Aug 16 11:45:04 2006 Subject: [Histonet] Chlamydia positive control Message-ID: <44E313250200007700001670@hcnwgwds01.hh.chs> Does anyone have control tissue for Chlamydia? Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From pathrm35 <@t> adelphia.net Wed Aug 16 12:17:41 2006 From: pathrm35 <@t> adelphia.net (pathrm35@adelphia.net) Date: Wed Aug 16 12:17:52 2006 Subject: [Histonet] Question about purchasing refurbished equipment Message-ID: <15221204.1155748661449.JavaMail.root@web20> I have had very good luck with Belair and very bad luck with Midwest Science Biocenter (MSB). I will never use MSB again because I found their products and service (Matt Fisher) to be unacceptable. I would highly recommend Belair. ---- Barbara Guidos wrote: > Hello All - > > I am looking to purchase a cytology/ histology slide stainer and would be interested in knowing if you have any experience (positive or negative) in purchasing refurbished laboratory equipment through biomedical corporations such as Rankin, LabX, etc. > > Feel free to send your comments to my email address below. > > Thanks for your help. > > Barb Guidos > > __________________________________ > Barbara J. Guidos, SCT(ASCP), CT(IAC) > UroPartners, LLC Laboratory > 2225 Enterprise Dr. Suite 2511 > Westchester, IL. 60154 > > (T) 708.486.0076 > (F) 708.486.0080 > bguidos@lab.uropartners.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RSRICHMOND <@t> aol.com Wed Aug 16 12:24:36 2006 From: RSRICHMOND <@t> aol.com (RSRICHMOND@aol.com) Date: Wed Aug 16 12:24:46 2006 Subject: [Histonet] Re: Immunostaining for H. pylori Message-ID: <55b.529e206.3214aed4@aol.com> Joseph Nerk (where?) asks: >>Does any in histo land can recommend a good Immunostaining detection kit for Helicobacter pylori, have been seeing a lit recently but the pathologist looks at the Giemsa but still will like the Immunostaining method.<< For the last year or so we've been using the Cell Marque polyclonal, with a Ventana stainer, and have had very little trouble with it. As a pathologist, my take on immunostaining Helicobacter is that it saves me a lot of time. With one stain a day and 20 cases to sign out, I don't mind searching a Giemsa or Diff-Quik II stain with an oil immersion lens, but if I've got 10 cases a day and 70 cases to sign out, it's a great help. Another point to consider: if you do the immunostain, you greatly increase the total number of immunostains you do. That can justify setting up immunohistochemistry in a pathology service that's otherwise too small to have it. Bob Richmond Gastonia NC From jessgrocki <@t> yahoo.com Wed Aug 16 12:35:56 2006 From: jessgrocki <@t> yahoo.com (Jessica Piche) Date: Wed Aug 16 12:36:06 2006 Subject: [Histonet] Anyone using Pascal? Message-ID: <20060816173556.73115.qmail@web82006.mail.mud.yahoo.com> Hello All, I was wondering if anyone can tell me if they are using Pascal (from Dako) for ER/PR? If so, how do you like it? Does it take longer than a steamer for antigen retrieval? What do you think of it? Any information you can provide would be great! Thanks!! Jessica Piche-Grocki, HT(ASCP) From Jackie.O'Connor <@t> abbott.com Wed Aug 16 12:58:32 2006 From: Jackie.O'Connor <@t> abbott.com (Jackie M O'Connor) Date: Wed Aug 16 12:59:09 2006 Subject: [Histonet] Anyone using Pascal? In-Reply-To: <20060816173556.73115.qmail@web82006.mail.mud.yahoo.com> Message-ID: I'm using the Pascal steamer for everything I used to use the microwave for. I like it because I can walk away from it, and know the approximate time the slides will be cooled off to remove and stain. If I forget, there is an alarm to remind me - or someone. Considering that all the slides are treated equally (I can do up to 48 slides at a time) unlike in a microwave - that may be worth the effort alone. Jackie O' Jessica Piche Sent by: histonet-bounces@lists.utsouthwestern.edu 08/16/2006 12:35 PM To histonet cc Subject [Histonet] Anyone using Pascal? Hello All, I was wondering if anyone can tell me if they are using Pascal (from Dako) for ER/PR? If so, how do you like it? Does it take longer than a steamer for antigen retrieval? What do you think of it? Any information you can provide would be great! Thanks!! Jessica Piche-Grocki, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From kaleid11 <@t> yahoo.com Wed Aug 16 13:01:44 2006 From: kaleid11 <@t> yahoo.com (Adam Perry) Date: Wed Aug 16 13:01:50 2006 Subject: [Histonet] Looking for Estrogen Receptor Beta Antibody In-Reply-To: <55b.529e206.3214aed4@aol.com> Message-ID: <20060816180144.37407.qmail@web30405.mail.mud.yahoo.com> Hello all, I was trying to find a good source for an ER beta antibody. I previously used the rabbit polyclonal from Zymed but apparently it is on backorder now. Need antibody for: estrogen receptor beta (ESR2) immunohistochemistry on formalin-fixed tissue (brain) must react with rat ER beta no cross-reactivity with ER alpha I'd prefer the host be rabbit (but not essential) I'd love to hear what people are using, especially to detect rat ERbeta in brain Thanks, Adam --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail Beta. From liz <@t> premierlab.com Wed Aug 16 13:18:41 2006 From: liz <@t> premierlab.com (Liz Chlipala) Date: Wed Aug 16 13:13:46 2006 Subject: [Histonet] pascal Message-ID: <000601c6c160$6711aa70$0300a8c0@domain.Premier> Jessica We use the pascal for all of our antibodies that require retrieval. Since we are in Colorado and at a higher elevation the highest temp we can get our retrieval solution in the steamer is about 93 to 94+ degrees. We still use our steamer if we have problems with tissues falling off, buts its more of an ordeal to make sure we are at the correct temp before we start. It takes over an hour for us to retrieve in the steamer since we monitor the temperature of the retrieval solution the entire time. We wait until the solution gets to 93 degrees before we place the slides in it and then continue to monitor, you will see a drop in temp once the slides are placed in the solution and then we wait until the solution is back up to 93 degrees and then we start the 20 minute retrieval process. During this entire process you need to also make sure that you don't run out of water, so we definitely like the pascal and use it at the factory settings with good reproducibility. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 From cheastys <@t> svm.vetmed.wisc.edu Wed Aug 16 13:18:08 2006 From: cheastys <@t> svm.vetmed.wisc.edu (Sandra Cheasty) Date: Wed Aug 16 13:19:03 2006 Subject: [Histonet] (no subject) Message-ID: Hello, We are in need of an inexpensive cassette labeler. Please let me know if you can help. Thanks, Sandy Sandra Cheasty Histology Supervisor UW-Madison School of Veterinary Medicine 608 263-1680 From pmarcum <@t> vet.upenn.edu Wed Aug 16 13:41:55 2006 From: pmarcum <@t> vet.upenn.edu (Pamela Marcum) Date: Wed Aug 16 13:42:07 2006 Subject: [Histonet] Pennsylvania Program for Ocotber Meeting Speaker Time Changes Message-ID: <6.1.1.1.2.20060816140610.019d6e20@mail.vet.upenn.edu> Good Afternoon All, We have had some speaker changes on our October program due to scheduling issues. The changes are on the program at our web site www.pahisto.org and will be listed here for all who have received a short program form by fax or e-mail. We also have changes on the registration form as we accidentally placed workshops 13, (The Bog People of Norhtern Europe) 14, Grossing Surgical Specimens, A Histologist View), and 15 (Wet Workshop: Multiple Antigen IHC Staining - This is also a day and time change from Saturday to Friday afternoon). If you wanted to take these workshops and have registered or were planning to take them please review the corrected registration sheet and contact either Gloria Limetti or me for changes. We are sorry about these changes however, schedules were beyond our control and the others are just reaching a point in reviewing the program that you see what you think should be there not what is written. We ran out of volunteers (like they had a choice) to edit and proof read before we had these corrections. Please Accept Our Apologies For Any Inconvience and Let Know If You See Anything Else! Thursday October 26 ? Special Management Seminars This day is designed for Supervisors and Managers to assist in some of the special areas we are all asked to become experts in today with limited resources and opportunities to attend local educational events. If you are a new manager or just need a refresher with updates join us!! WS# 1 8:00AM to 11:30AM CPT Coding - Which Code Do I Use? Whitaker WS# 2 1:00PM to 4:30PM Preparing for a CAP Inspection Nocito & Hernandez WS# 3 4:30PM to 6:30PM The SWEET Workshop Smart Working Environment Ergonomics Training Minshew Friday Oct 27 - Morning Sessions WS# 4 8:00AM to 9:30 AM Richmond Introduction to Fine Needle Aspiration for the Histotechnologist WS# 5 10:00AM to 11:30 AM Histology Laboratory Workload Measurement: Evaluating Complexity and Productivity Schmitt WS# 6 8:00AM to 11:30 AM Don?t Let Immunos Intimidate You! Whitaker Beginning Immunos and Refresher WS# 7 8:00AM to 9:30 AM Safety a Priority in Our Lives Casey WS# 8 10:00AM to 11:30 AM Recycling for Histology Welch WS# 9 8:00AM to 11:30AM Praet Unlocking the Secrets of Mohs? Grossing and Cryosectioning Friday October 27 ? Afternoon Sessions WS#10 1:00PM to 2:30 PM Smart Shopping and Contracts for Histology Macrea WS# 11 1:00PM to 2:30 PM Detection and Amplification of Nucleic Acids in Morphologically Preserved Cells and Tissues Gore WS# 12 1:00PM to 2:30PM Automated Stains Workshop with Multiple Vendors (15 minutes each) WS# 13 3:00PM to 4:30 PM The Bog People of Northern Europe Olsen WS#14 1:00PM to 4:30 PM Nocito & Grossing Surgical Specimens: A Histologist View Hernandez WS# 15 1:00PM to 4:30 PM Meyers Wet Workshop: Multi-antigen Immunohistochemistry (IHC) Staining Saturday October 28 - Morning Sessions WS#16 8:00AM to 11:30 AM Brave New World: An Introduction to Molecular Pathology Haas WS#17 8:00AM to 11:30 AM Peters Frozen Section Techniques New Methods for Cryo-Embedding WS#18 8:00AM to 9:30 AM The Art of Forensic Autopsy Mancuso WS #19 8:00AM to 4:30AM QIHC Readiness and Troubleshooting Macrea WS# 20 8:00AM to 4:30PM HT (ASCP) Examination Readiness Micciche Saturday October 28 Afternoon Sessions WS# 21 1:00PM to 2:30 PM What Plastic Do I Embed This Project In? Marcum WS# 22 1:00PM to 2:30 PM Specimen Labeling and Identification Bar Codes TBA WS#23 1:00PM to 2:30 PM Forensic Insect Entomology Todaro Best Regards, Pamela A Marcum Manager, Histology Special Procedures University of Pennsylvania School of Veterinary Medicine R.S. Reynolds Jr. CORL New Bolton Center 382 West Street Road Kennett Square, PA 19348 Phone - 610-925-6278 Fax - 610-925-8120 E-mail - pmarcum@vet.upenn.edu From tkngflght <@t> yahoo.com Wed Aug 16 13:49:14 2006 From: tkngflght <@t> yahoo.com (Cheryl) Date: Wed Aug 16 13:49:18 2006 Subject: [Histonet] Question about purchasing refurbished equipment In-Reply-To: <15221204.1155748661449.JavaMail.root@web20> Message-ID: <20060816184914.51705.qmail@web50904.mail.yahoo.com> I have purchased Belair used equipment in the past as well. They state clearly the condition, it was well serviced and we saved quite a bit on our cap budget. There is also a fellow who occasionally posts on here--don't know anything about him but he seems to have quite a range of equipment--Ford Royer I think is his name? Cheryl pathrm35@adelphia.net wrote: I have had very good luck with Belair and very bad luck with Midwest Science Biocenter (MSB). I will never use MSB again because I found their products and service (Matt Fisher) to be unacceptable. I would highly recommend Belair. ---- Barbara Guidos wrote: > Hello All - > > I am looking to purchase a cytology/ histology slide stainer and would be interested in knowing if you have any experience (positive or negative) in purchasing refurbished laboratory equipment through biomedical corporations such as Rankin, LabX, etc. > > Feel free to send your comments to my email address below. > > Thanks for your help. > > Barb Guidos > > __________________________________ > Barbara J. Guidos, SCT(ASCP), CT(IAC) > UroPartners, LLC Laboratory > 2225 Enterprise Dr. Suite 2511 > Westchester, IL. 60154 > > (T) 708.486.0076 > (F) 708.486.0080 > bguidos@lab.uropartners.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From ander093 <@t> tc.umn.edu Wed Aug 16 13:51:32 2006 From: ander093 <@t> tc.umn.edu (LuAnn Anderson) Date: Wed Aug 16 13:51:42 2006 Subject: [Histonet] Question about purchasing refurbished equipment In-Reply-To: <20060816184914.51705.qmail@web50904.mail.yahoo.com> References: <15221204.1155748661449.JavaMail.root@web20> <20060816184914.51705.qmail@web50904.mail.yahoo.com> Message-ID: <6.2.3.4.0.20060816135052.03939068@ander093.email.umn.edu> Yup---I sent Barbara Ford's information. He is great!!! At 01:49 PM 8/16/2006, Cheryl wrote: >I have purchased Belair used equipment in the past as well. They >state clearly the condition, it was well serviced and we saved quite >a bit on our cap budget. > > There is also a fellow who occasionally posts on here--don't know > anything about him but he seems to have quite a range of > equipment--Ford Royer I think is his name? > > Cheryl > >pathrm35@adelphia.net wrote: > I have had very good luck with Belair and very bad luck with > Midwest Science Biocenter (MSB). I will never use MSB again because > I found their products and service (Matt Fisher) to be > unacceptable. I would highly recommend Belair. >---- Barbara Guidos wrote: > > Hello All - > > > > I am looking to purchase a cytology/ histology slide stainer and > would be interested in knowing if you have any experience (positive > or negative) in purchasing refurbished laboratory equipment through > biomedical corporations such as Rankin, LabX, etc. > > > > Feel free to send your comments to my email address below. > > > > Thanks for your help. > > > > Barb Guidos > > > > __________________________________ > > Barbara J. Guidos, SCT(ASCP), CT(IAC) > > UroPartners, LLC Laboratory > > 2225 Enterprise Dr. Suite 2511 > > Westchester, IL. 60154 > > > > (T) 708.486.0076 > > (F) 708.486.0080 > > bguidos@lab.uropartners.com > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jennifer.l.hofecker <@t> Vanderbilt.Edu Wed Aug 16 13:54:22 2006 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Wed Aug 16 13:58:48 2006 Subject: [Histonet] Question about purchasing refurbished equipment References: <5DA1CA5D0B98A84985B545A24423B822021DDD@UPLAB01.uplab.local> Message-ID: <898D946569A27444B65667A49C074052852E7B@mailbe06.mc.vanderbilt.edu> Hi Barb, There are several good companies out there. Recently, we have had a great purchasing experience with IMEB. Their number is 800-543-8496. They were very good about calling and letting us know when things became available but were not pushy at all. It's definitely worth a call! Good luck. Jennifer Hofecker, HT (ASCP) Vanderbilt University Medical Center Division of Neuropathology (615) 343-0083 (615) 343-7089 fax ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Barbara Guidos Sent: Wed 8/16/2006 11:41 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Question about purchasing refurbished equipment Hello All - I am looking to purchase a cytology/ histology slide stainer and would be interested in knowing if you have any experience (positive or negative) in purchasing refurbished laboratory equipment through biomedical corporations such as Rankin, LabX, etc. Feel free to send your comments to my email address below. Thanks for your help. Barb Guidos __________________________________ Barbara J. Guidos, SCT(ASCP), CT(IAC) UroPartners, LLC Laboratory 2225 Enterprise Dr. Suite 2511 Westchester, IL. 60154 (T) 708.486.0076 (F) 708.486.0080 bguidos@lab.uropartners.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Xilong.Li <@t> UTSouthwestern.edu Wed Aug 16 14:25:40 2006 From: Xilong.Li <@t> UTSouthwestern.edu (Xilong Li) Date: Wed Aug 16 14:25:57 2006 Subject: [Histonet] looking for a method to quantitative evaluation of histological image Message-ID: <44E32AE4020000F000005BAC@swnw124.swmed.edu> Hi, All, I am looking for a consistent method to quantitative evaluation of histological image. In our labs, we conducted the research on dystrophy muscle, after experimental treatment, damage in skeletal muscle supposed to be improved such as fiber size or fiberosis changing, H&E staining or trichrome staining were used on frozen section to assess the improvement of damage, but it is hard to say how much of damage were improved. I just wonder is there any microcomputer-based system or scale can be used to do semiquantitative image analysis? Please let me know if possible. Thanks in advance. Xilong li Dr. Xilong Li Hypertension Division, Internal Medicine University of Texas Southwestern Medical Center 5323 Harry Hiness Blvd-J4.142 Dallas, TX 75390 Tel: 214-648-9966(L) Fax: 214-648-7902 From PKamalavenkatesh <@t> wockhardtin.com Wed Aug 16 22:09:55 2006 From: PKamalavenkatesh <@t> wockhardtin.com (PKamalavenkatesh@wockhardtin.com) Date: Wed Aug 16 22:07:04 2006 Subject: [Histonet] nile red staining-phospholipidosis-problems-reg Message-ID: Dear Histonetters, This is with regard to detection of phospholipidosis using nile red staining by fluorescence microscopy. I have seen a number of discussions regarding this in our archive. Actually we have induced phospholipidosis in rats using azithromycin since this drug is known for inducing systemic phospholipidosis. We have taken cryosections of liver (10 microns thickness) and stain the section with a drop of nile red (Sigma) initially dissolved in acetone and then diluted with Glycerol water mixture (75:25). The final concentration of nile red is 1 microgram per ml. We are able to get two types of fluorescence one yellowish gold (neutral lipids) and the other orange red (phospholipids). The reference is Brown et al., Nile red staining of lysosomal phospholipid inclusions, Histochemistry, 97: 349-354. Now the problems I am facing are 1.Since the staining is done with glycerol water mixture, we are not able to make the permanent preparations and also not able to examine the slides under oil immersion. 2.If there is any other protocol that does not involve glycerol water mixture. Hence we can stain the sections with neutral red, wash the sections and then mount the sections. 3.Anybody can suggest me an aqueous mounting medium that will aid in making permanent preparations. I also want to know if anybody is encountering the same problems Regards DR.P.KAMALAVENKATESH NEW DRUG DISCOVERY-BIOLOGY PRE CLINICAL SAFETY ASSESSMENT DIVISION WOCKHARDT RESEARCH CENTER AURANGABAD MAHARASHTRA, INDIA E.mail: PKamalavenkatesh@wockhardtin.com Please Visit our New Corporate Web Site www.wockhardt.com --------------------- Disclaimer ------------------------------------------------------------------------------ Information transmitted by this E-MAIL is proprietary to Wockhardt Ltd. and/ or its Group Companies and/or,its Customers and is intended for use only by the individual or entity to which it is addressed, and may contain information that is privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. --------------------------------------------------------------------------------------------------------------- From hakim <@t> bgu.ac.il Thu Aug 17 06:26:41 2006 From: hakim <@t> bgu.ac.il (Vicki Hakim) Date: Thu Aug 17 06:26:54 2006 Subject: [Histonet] embedding protocol for embryos Message-ID: hi all does any one know a good protocol for embedding young mouse embryos( E-8/9)? the embryos are used for mRNA in-situ hybridizations. i have been trying different washing protocols and the tissue is always porous. if anyone could comment on fixation time and the time recommended for such small tissue to be in the melted paraffin that would be great. thanks vicki ? From TMcNemar <@t> lmhealth.org Thu Aug 17 06:33:53 2006 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Thu Aug 17 06:34:55 2006 Subject: [Histonet] Immunostaining for H.pylori Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F3E8@lmhsmail.lmhealth.org> We have used Dako's for a number of years with good results. Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Joseph Nerk Sent: Tuesday, August 15, 2006 10:42 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Immunostaining for H.pylori Does any in histo land can recommend a good Immunostaining detection kit for Helicobacter pylori, have been seeing a lit recently but the pathologist looks at the Giemsa but still will like the Immunostaining method. Awaiting your response. _________________________________________________________________ Express yourself instantly with MSN Messenger! [1]MSN Messenger Download today it's FREE! References 1. http://g.msn.com/8HMBEN/2740??PS=47575 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From sheila_adey <@t> hotmail.com Thu Aug 17 07:35:05 2006 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Thu Aug 17 07:35:11 2006 Subject: [Histonet] Re: Immunostaining for H. pylori In-Reply-To: <55b.529e206.3214aed4@aol.com> Message-ID: Hi, We have been using the AEC polymer detectiong kit for the Biogenex i6000 and find good staining with that. Can anyone tell me if they have any experience with the Biocare Nemesis 7200, they say that they offer true random access? Not just continuous access? Any comments would be greatly appreciated >From: RSRICHMOND@aol.com >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Re: Immunostaining for H. pylori >Date: Wed, 16 Aug 2006 13:24:36 EDT > >Joseph Nerk (where?) asks: > > >>Does any in histo land can recommend a good Immunostaining detection kit >for Helicobacter pylori, have been seeing a lit recently but the >pathologist >looks at the Giemsa but still will like the Immunostaining method.<< > >For the last year or so we've been using the Cell Marque polyclonal, with a >Ventana stainer, and have had very little trouble with it. As a >pathologist, my >take on immunostaining Helicobacter is that it saves me a lot of time. With >one stain a day and 20 cases to sign out, I don't mind searching a Giemsa >or >Diff-Quik II stain with an oil immersion lens, but if I've got 10 cases a >day >and 70 cases to sign out, it's a great help. > >Another point to consider: if you do the immunostain, you greatly increase >the total number of immunostains you do. That can justify setting up >immunohistochemistry in a pathology service that's otherwise too small to >have it. > >Bob Richmond >Gastonia NC >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Play Q6 for your chance to WIN great prizes. http://q6trivia.imagine-live.com/enca/landing From MDiCarlo <@t> KaleidaHealth.Org Thu Aug 17 07:48:00 2006 From: MDiCarlo <@t> KaleidaHealth.Org (DiCarlo, Margaret) Date: Thu Aug 17 07:48:08 2006 Subject: [Histonet] cleaning disinfectant Message-ID: <9B4A77DF11463E4FB723D484214AE9BC01521683@KALEXMB02.KaleidaHealth.org> Hello Histonetters, Could anyone recommend a disinfectant that is non-corrosive for cleaning instruments? I currently use diluted bleach after cutting bone on a band saw and I was hoping there is something better out there to use. Any ideas would be greatly appreciated. Peggy DiCarlo HT(ASCP) Orthopedics Bone Lab Buffalo General Hospital 100 High St. Buffalo, NY 14203 716-859-1293 CONFIDENTIALITY NOTICE: This email transmission and any documents, files, or previous e-mail messages attached to it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, or a person responsible for delivering it to the intended recipient, you are hereby notified that any further review, disclosure, copying, dissemination, distribution, or use of any of the information contained in or attached to this e-mail transmission is strictly prohibited. If you have received this message in error, please notify the sender immediately by e-mail, discard any paper copies, and delete all electronic files of the message. If you are unable to contact the sender or you are not sure as to whether you are the intended recipient, please e-mail ISTSEC@KaleidaHealth.org or call (716) 859-7777. From cpomajzl <@t> cpllabs.com Thu Aug 17 08:03:50 2006 From: cpomajzl <@t> cpllabs.com (Chris Pomajzl) Date: Thu Aug 17 07:58:00 2006 Subject: [Histonet] embedding protocol for embryos References: Message-ID: <001201c6c1fd$995f15b0$26fca8c0@CSP> Vicki, When I was in research, we would process very young embryos for RNA ISH as well. We would fix the embryos while still in the decidua. In fact, at E8-E9, we would simply cut the uterus between each embryo and have them drop into normal saline and then transferred to fixative. These were fixed in 4% PFA overnight in 4'C refrigerator on a rocking platform. Embryos were transferred to normal saline or PBS the next day. Once the embryos are harvested from the decidua, they are hand-process with graded ethanols and xylene. 3-5 minutes is sufficient for each step with slight agitation to facilitate diffusion. Three paraffin changes at 5-10 minutes followed by embedding. Hope this helps. Chris Pomajzl, HTL (ASCP) Histology Supervisor Clinical Pathology Laboratories, Inc. 9200 Wall Street Austin, Texas 78754 512.873.1660 (o) 512.873.5004 (f) cpomajzl@cpllabs.com CONFIDENTIALITY NOTICE: This communication is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law. If you are not the intended recipient, you are notified that any use, dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this message in error, please notify the sender immediately and destroy this communication. ----- Original Message ----- From: "Vicki Hakim" To: Sent: Thursday, August 17, 2006 6:26 AM Subject: [Histonet] embedding protocol for embryos hi all does any one know a good protocol for embedding young mouse embryos( E-8/9)? the embryos are used for mRNA in-situ hybridizations. i have been trying different washing protocols and the tissue is always porous. if anyone could comment on fixation time and the time recommended for such small tissue to be in the melted paraffin that would be great. thanks vicki ? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From michael.owen <@t> fda.hhs.gov Thu Aug 17 08:50:49 2006 From: michael.owen <@t> fda.hhs.gov (Owen, Michael P) Date: Thu Aug 17 08:51:37 2006 Subject: [Histonet] cleaning disinfectant Message-ID: Dear Peggy, The selection process of a disinfectant depends on the target microorganisms and the type of disinfection (pre-cleaning, primary disinfection, terminal disinfection, etc.) needed. I am a novice to the details of disinfection, therefore I recommend you contact the American Biological Safety Association (ABSA), your facility's biological safety officer (BSO) or a BSO at a university near your location. You may be able to use a quaternary ammonium compound in combination with an alcohol for pre-cleaning then perform a sterilization using an autoclave or ethylene oxide (the sterilization performed on the removable parts that have direct contact with specimens). I have been outside of the hospital environment for a while, therefore these suggestions are guesses. Please e-mail me if you need to contact more individuals in the biosafety field who could answer this question better than I can. Sincerely, Lab Rat Michael P. Owen, Regulatory Microbiologist U.S. FDA Pacific Regional Lab Northwest 22201 23rd Drive SE Bothell, WA 98021-4421 Phone: 425-483-4865 E-Mail: michael.owen@fda.hhs.gov From vazquezr <@t> ohsu.edu Thu Aug 17 09:09:54 2006 From: vazquezr <@t> ohsu.edu (Robyn Vazquez) Date: Thu Aug 17 09:10:40 2006 Subject: [Histonet] cleaning disinfectant Message-ID: I use Sanimaster 4, Google it and get pricing. Robyn OHSU From SBaldwin <@t> compucyte.com Thu Aug 17 09:12:09 2006 From: SBaldwin <@t> compucyte.com (Scott Baldwin) Date: Thu Aug 17 09:13:40 2006 Subject: [Histonet] University of Texas MD Anderson Seminar Message-ID: From: Scott Baldwin (CompuCyte Corporation) sbaldwin@compucyte.com Subject: University of Texas MD Anderson Seminar To the researchers in the Texas area: There will be quantitative imaging cytometry seminar at MD Anderson on August 31. Agenda: 1. Welcome - Michael Andreeff, Chief, Section of Molecular Hematology and Therapy Director of Research, Department of Blood and Marrow Transplantation University of Texas M.D. Anderson Cancer Center 2. "Latest Advances in Laser Scanning Cytometry: iCyte, iCys, and iColor" Ed Luther, Principal Scientist, CompuCyte Corporation 3. "Assessment of DNA Damage by Various Cytometry Techniques" Zbigniew Darzynkiewicz, Director, Brander Cancer Research Institute, New York Medical College, Valhalla, NY 4. "One Step Closer to the Understanding of Mechanisms of DNA Replication in Human Tumors" John Lehman, Associate Vice-Chancellor for Research Professor, Pathology and Laboratory Medicine, Brody School of Medicine, East Carolina University, NC 5. Demonstration of the iCys? Research Imaging Cytometer at the Department of Epidemiology Basic Science Research Building. The program will also cover subjects of FISH, CISH and automated tissue analysis applied in the areas of diagnostic and pharmcodynamic biomarker validation and investigative pathology. More information is available at www.compucyte.com. I hope that many of you will find this seminar of interest and value. From PMonfils <@t> Lifespan.org Thu Aug 17 09:28:33 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Thu Aug 17 09:28:42 2006 Subject: [Histonet] marking a tissue block Message-ID: <09C945920A6B654199F7A58A1D7D1FDE01717782@lsexch.lsmaster.lifespan.org> India ink, available at any art supply store, can be applied to the external surface of a tissue sample. It is unaffected by the processing solvents. It diesn't penetrate into the tissue, so it doesn't interfere with any subsequent staining techniques. If it is applied to what will be the "sides" of the sample when it is embedded, then it will appear in the section as a thin black line on the outer surface. So, sor example, if you are cutting a longitudinal section of a rat intestine, and you want to keep track of which end is proximal and which end is distal, apply ink around one end of the intestine, and when sectioned that end can be identified by the black outline, without interfering with the view of the tissue itself. Tissue should be removed from the fixative, blotted with paper towels until it is just damp. Otherwise the ink will run all over the place. I apply the ink with a thin wooden applicator stick, let it sit on the tissue for about 10-15 seconds, then blot the ink with the paper towel and return the specimen to the fixative. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of > christa hercher > Sent: Wednesday, August 16, 2006 6:17 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] marking a tissue block > > Hello Histonets, > I was wondering if anyone has suggestions on how to mark a block of > tissue in order to orient yourself when looking at the sections under the > microscope. The mark will have to go through the entire block of tissue. > The tissue is also being stained with golgi so the mark has to be > something that will not react with the stain. Ideas and comments would be > greatly appreciated. > > smiles, > > Christa Hercher > McGill University > > > "Don't blame it on the sunshine, don't blame it on the moonlight, don't > blame it on good times, blame it on the boogie." > Captain Jack > > --------------------------------- > Be smarter than spam. See how smart SpamGuard is at giving junk email the > boot with the All-new Yahoo! Mail > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From ski63 <@t> mchsi.com Fri Aug 4 21:58:13 2006 From: ski63 <@t> mchsi.com (ski63) Date: Thu Aug 17 10:44:35 2006 Subject: [Histonet] Lymph Node Revealing Solution Message-ID: <001401c6b83b$09732480$3517d60c@john> Laurie, Your pathologist may be referring to Carnoy's fixative. It is composed of chloroform, acetic acid and and alcohol. When specimens are placed in the Carnoy's, the lymph nodes will turn "white". The tissue sections can then be placed in 10% NBF and processed as usual. Hope this info helps. Dawn Olszewski, HTL (ASCP) QIHC South Georgia Medical Center Valdosta, GA. From LSebree <@t> uwhealth.org Thu Aug 17 11:49:44 2006 From: LSebree <@t> uwhealth.org (Sebree Linda A.) Date: Thu Aug 17 11:49:54 2006 Subject: [Histonet] Collagen testing Message-ID: > Hello histonetters, > > A researcher at our institution is looking for a reference lab that > performs collagen testing by IHC. I know of Collagen Types I, II, III > & IV but don't know if there are any others. If there are reference > labs out there that perform more than just Type IV, please let me know > and I'll pass the information along. > > Thank you, > > Linda Sebree, HT(ASCP) > University of Wisconsin Hospital & Clinics > IHC/ISH Laboratory > A4/204-3224 > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > FAX: (608)262-7174 > From liz <@t> premierlab.com Thu Aug 17 12:10:29 2006 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Aug 17 12:05:35 2006 Subject: [Histonet] Collagen testing In-Reply-To: Message-ID: <000001c6c220$0a735370$0300a8c0@domain.Premier> Linda We can run Collagen I, II, III, IV and VII for immunohistochemistry, We are currently working on Collagen X, we also can run aggrecan and then markers for dermis specimens such as talin, vitronectin, etc. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A. Sent: Thursday, August 17, 2006 10:50 AM To: Histonet Subject: [Histonet] Collagen testing > Hello histonetters, > > A researcher at our institution is looking for a reference lab that > performs collagen testing by IHC. I know of Collagen Types I, II, III > & IV but don't know if there are any others. If there are reference > labs out there that perform more than just Type IV, please let me know > and I'll pass the information along. > > Thank you, > > Linda Sebree, HT(ASCP) > University of Wisconsin Hospital & Clinics > IHC/ISH Laboratory > A4/204-3224 > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > FAX: (608)262-7174 > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1.1712 (20060817) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com From Michael.Rice <@t> holy-cross.com Thu Aug 17 12:38:37 2006 From: Michael.Rice <@t> holy-cross.com (Rice, Michael) Date: Thu Aug 17 12:38:45 2006 Subject: [Histonet] purchasing refurbished equipment Message-ID: <3BC92F29BE821745AB15E04C98EE028D6D3964@HCH2KMAIL.holy-cross.com> The main idea, is to find and use a company that will guarantee its equipment, and will furnish a list of references. I have used Imeb on several occasions for refurbished items and have always been satisfied with their level of service. Michael Rice CT.HT(ASCP) Supervisor Of Pathology Holy Cross Hospital Ft Lauderdale, Fl 33308 954.776.3070 ====================== Confidentiality Notice: Prepared in Anticipation of Litigation. Attorney-Client Privileged. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original. ====================== From Shirley_PHUA <@t> hsa.gov.sg Thu Aug 17 13:06:13 2006 From: Shirley_PHUA <@t> hsa.gov.sg (Shirley PHUA) Date: Thu Aug 17 13:08:58 2006 Subject: [Histonet] Shirley is away : 18 August 2006 (Friday) Message-ID: I will be out of the office from 08/18/2006 to 08/18/2006. I will return on 21 August 2006 (Monday). Pathologists : I will process your requests when I return. Otherwise, if urgent, please forward your mail to henry_kyaw@hsa.gov.sg Regrets for the inconveniences caused. From Steven2146 <@t> aol.com Thu Aug 17 13:26:46 2006 From: Steven2146 <@t> aol.com (Steven2146@aol.com) Date: Thu Aug 17 13:26:54 2006 Subject: [Histonet] Re: Histonet Digest, Vol 33, Issue 22 Message-ID: HI... If you're looking for reliable refurbished histology equipment, Belair is the best out there....the machines not only are TOTALLY reconditioned, but when you receive them, they look brand new....Belair, as far as I know, is the only company out there selling refurbished equipment, that offers a one year, unconditional guarantee. Steven Lee HT ASCP From prouty27 <@t> msn.com Thu Aug 17 14:13:14 2006 From: prouty27 <@t> msn.com (Sally Prouty) Date: Thu Aug 17 14:13:24 2006 Subject: [Histonet] mouse eyes In-Reply-To: Message-ID: Does anyone have a protocol for processing mouse eyes that doesn't cause them to end up looking like raisins? Besides hand processing we haven't find a consistant method. Sally J. Prouty Research Assistant, Campbell Laboratory Howard Hughes Medical Institute Department of Physiology & Biophysics University of Iowa College of Medicine 4283 Carver Biomedical Research Building Iowa City, IA 52242-1101 _________________________________________________________________ Express yourself instantly with MSN Messenger! Download today - it's FREE! http://messenger.msn.click-url.com/go/onm00200471ave/direct/01/ From portera <@t> msu.edu Thu Aug 17 14:23:21 2006 From: portera <@t> msu.edu (Amy Porter) Date: Thu Aug 17 14:22:15 2006 Subject: [Histonet] CAP Inspection question for Proficiency Testing in Histology Message-ID: <002301c6c232$99d82330$8e7a0923@HistoJJ> Can anyone out there is histoland tell me how they are meeting the proficiency testing program requirements for CAP inspections specifically with regards to histology..... I have spoken with CAP about the HistoQIP program and they consider that an educational program, not a proficiency program. Just wondering what other options are out there if any. Thanks for your responses in advance! Amy S. Porter, HT(ASCP) QIHC - Supervisor Michigan State University Investigative HistoPathology Laboratory Department of Physiology / Division of Human Pathology 2100 Biomedical Physical Sciences Bldg. Room #2133 East Lansing, MI 48824-3320 Phone: (517) 355-6475 ext 1480 / Fax: (517) 432-1368 Email: portera@msu.edu www.humanpathology.msu.edu From rjbuesa <@t> yahoo.com Thu Aug 17 14:40:46 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Aug 17 14:40:52 2006 Subject: [Histonet] CAP Inspection question for Proficiency Testing in Histology In-Reply-To: <002301c6c232$99d82330$8e7a0923@HistoJJ> Message-ID: <20060817194046.17625.qmail@web61216.mail.yahoo.com> Amy: I used to have "competency check lists" and "standard of performance descriptions" for each mayor task. Along the year I used to observe the activity of each HT in each task and evaluated them against those lists. The deficiencies I found were documented and used for the individual PI of each HT, which went to their personal file. When during the CAP inspections I was asked about this aspect I showed them the PI developed for each HT that required a re-training on any given task. They were pleased with this approach to documenting the proficiency in completing histology tasks. Hope this will help you! Ren? J. Amy Porter wrote: Can anyone out there is histoland tell me how they are meeting the proficiency testing program requirements for CAP inspections specifically with regards to histology..... I have spoken with CAP about the HistoQIP program and they consider that an educational program, not a proficiency program. Just wondering what other options are out there if any. Thanks for your responses in advance! Amy S. Porter, HT(ASCP) QIHC - Supervisor Michigan State University Investigative HistoPathology Laboratory Department of Physiology / Division of Human Pathology 2100 Biomedical Physical Sciences Bldg. Room #2133 East Lansing, MI 48824-3320 Phone: (517) 355-6475 ext 1480 / Fax: (517) 432-1368 Email: portera@msu.edu www.humanpathology.msu.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail Beta. From ROrr <@t> enh.org Thu Aug 17 15:01:09 2006 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Thu Aug 17 15:01:16 2006 Subject: [Histonet] Pascal Message-ID: We use the Biocare Decloaker in our lab. It's the same instrument as the Pascal. We use Dako ER/PR on our lab. We can run 4 tissue tek slide rack/containers at a time for a total of 98 slides in one run. Hope this helps. Becky Orr, CLA, HT(ASCP) QIHC Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2763 Hello All, I was wondering if anyone can tell me if they are using Pascal (from Dako) for ER/PR? If so, how do you like it? Does it take longer than a steamer for antigen retrieval? What do you think of it? Any information you can provide would be great! Thanks!! Jessica Piche-Grocki, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ ***************** From antyler <@t> uncg.edu Thu Aug 17 15:28:59 2006 From: antyler <@t> uncg.edu (Amber Tyler ANTYLER) Date: Thu Aug 17 15:28:45 2006 Subject: [Histonet] Ultra-Low Freezer Recommendation Message-ID: Hello, Has anyone had any experience with ultra-low freezers by Sanyo. I already know about rev-co, but I found a comparable freezer by sanyo that is a bit cheaper. Thank you, Amber Amber N. Tyler, Ph.D. Research Scientist Dept of Psychology (PO Box 26170) 296 Eberhart Building, Walker Avenue University of North Carolina - Greensboro Greensboro, NC 27402 From Reuel.Cornelia <@t> tsrh.org Thu Aug 17 16:44:10 2006 From: Reuel.Cornelia <@t> tsrh.org (Reuel Cornelia) Date: Thu Aug 17 16:46:56 2006 Subject: [Histonet] Salary in Texas Message-ID: Just wanted to hear some feedback on latest Wage data for Histotech supervisors particularly in dallas,texas. I want specifics, either per hour or salaried.Thank you. ******************************************************************************************************************* Texas Scottish Rite Hospital for Children is one of the nation's leading pediatric centers for the treatment of orthopedic conditions, certain related neurological disorders and learning disorders, such as dyslexia This email transmission and/or its attachments may contain confidential health information, intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing it to any other party unless required to do so by law and is required to delete/destroy the information after its stated need has been fulfilled. If you are not the intended recipient, any disclosure, copying, distribution or action taken in reliance on the contents of this email transmission is prohibited. If you have received this information in error, please notify the sender immediately and delete this information. We appreciate your efforts to protect the children's confidential information. ******************************************************************************************************************* From kfineout <@t> hotmail.com Thu Aug 17 17:02:22 2006 From: kfineout <@t> hotmail.com (Kelly Larson) Date: Thu Aug 17 17:02:33 2006 Subject: [Histonet] Refurbished Equipment Message-ID: We purchased a used Tissue-Tek processor from Rankin. It had some problems to start with but they stood by their product and fixed it for us free of charge. They seem to be pretty honest people to work with. From lanbergld <@t> vcu.edu Thu Aug 17 22:18:09 2006 From: lanbergld <@t> vcu.edu (Lawrence D Lanberg/O/VCU) Date: Thu Aug 17 22:18:17 2006 Subject: [Histonet] reply to Mouse Eyes Message-ID: Dear Ms. Prouty, I do mouse eyes -- ev looking like raisins, do you me wrinkle???? Or are you simply referring th Osmium Tetroxide imparts? I am always developing our protocols, but my ultra-thin sections come out more specifi Larry Lanberg PO Sanger Hall 2-032 Richmond, VA&n 804.828.7974 804.648.3246 From sulekhababy <@t> yahoo.co.uk Fri Aug 18 04:19:30 2006 From: sulekhababy <@t> yahoo.co.uk (Sulekha Ravi) Date: Fri Aug 18 04:19:38 2006 Subject: [Histonet] Why tissue sections from resin blocks have black deposits? Message-ID: <20060818091930.79205.qmail@web25009.mail.ukl.yahoo.com> Dear members I have a problem with my resin blocks.We make resin blocks for non decalcified bone and vessel with metallic stent in my research laboratory. We dehydrate the tissue in ascending grades of alcohol, impregnate with methyl methacrylate monomer and then make blocks in polymerised methyl methacrylate.Some areas of tissue in these blocks have white opaque precipitate like dirt in it. When sections from such blocks are seen under microscope, these areas are black precipitate like deposits, masking the entire cellular architecture. We tried sonicating the sections in water with and without detergents. But nothing helped. Please help me to rectify this serious problem. Thanks Sulekha. Sr. Technical Asst SCTIMST Trivandrum Kerala India 695012 --------------------------------- Copy addresses and emails from any email account to Yahoo! Mail - quick, easy and free. Do it now... From pex0220 <@t> yahoo.com.cn Fri Aug 18 05:55:32 2006 From: pex0220 <@t> yahoo.com.cn (pex) Date: Fri Aug 18 05:55:41 2006 Subject: [Histonet] Alizarin red staining Message-ID: <20060818105532.40790.qmail@web15212.mail.cnb.yahoo.com> Hello, I would like to perform Alizarin Red staining for mineralization nodules. Can someone do me a favor to show your protocol about it ? Thank you. Guofeng --------------------------------- ŃĹ»˘Ăâ·ŃÓĘĎä-3.5GČÝÁżŁ¬20M¸˝ĽţFrom michele.french <@t> bms.com Fri Aug 18 08:50:35 2006 From: michele.french <@t> bms.com (Michele French) Date: Fri Aug 18 08:53:00 2006 Subject: [Histonet] Muscle ATPase: Barbital Substitute Message-ID: <44E5C5AB.4070702@bms.com> Good Morning Everyone! I searched the archives and saw a few messages back in 2004 about using Glycine as a substitute for Sodium Barbital in the ATPase Stain for muscle fiber typing. The original e-mail did not include a recipe for the replacement of the barbital acetate preincubation solutions. It only talked about one preincubation solution at pH 9.4. I was wondering if anyone currently uses this substitute successfully and could send me your procedure? Thanks! Michele French Bristol-Myers Squibb Lawreceville, NJ From contact <@t> excaliburpathology.com Fri Aug 18 09:49:57 2006 From: contact <@t> excaliburpathology.com (Paula Pierce) Date: Fri Aug 18 09:50:02 2006 Subject: [Histonet] Mouse eyes Message-ID: <20060818144958.28339.qmail@web50107.mail.yahoo.com> I process to paraffin and cut whole eyes from everything from drosophila to ostrich with no retinal detachment or shattered lenses. How are you processing now? Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-759-3953 contact@excaliburpathology.com www.excaliburpathology.com From steven2146 <@t> aol.com Fri Aug 18 12:12:06 2006 From: steven2146 <@t> aol.com (steven2146@aol.com) Date: Fri Aug 18 12:12:29 2006 Subject: [Histonet] Re: Histonet Digest, Vol 33, Issue 23 In-Reply-To: <200608181300.58644e5f24892@rly-xk03.mx.aol.com> References: <200608181300.58644e5f24892@rly-xk03.mx.aol.com> Message-ID: <8C8910090D30174-148-42F3@MBLK-M32.sysops.aol.com> Hi... I've been a histotech in Florida for years and have purchased quite a number of reconditioned/refurbished instruments from Belair Instruments...there also happen to be the only remanufacturer of Histology laboratory equipment that comes with a full one year unconditional guarantee...when you see their instruments, you'd swear they were brand new!! The number for Belair is: 973-912-8900. Ask for Mike McDougal. He is the national sales manager and a pleasure to do business with.... Steven Lee HTASCP -----Original Message----- From: histonet-request@lists.utsouthwestern.edu To: histonet@lists.utsouthwestern.edu Sent: Fri, 18 Aug 2006 1:01 PM Subject: Histonet Digest, Vol 33, Issue 23 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-request@lists.utsouthwestern.edu You can reach the person managing the list at histonet-owner@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Collagen testing (Liz Chlipala) 2. purchasing refurbished equipment (Rice, Michael) 3. Shirley is away : 18 August 2006 (Friday) (Shirley PHUA) 4. Re: Histonet Digest, Vol 33, Issue 22 (Steven2146@aol.com) 5. mouse eyes (Sally Prouty) 6. CAP Inspection question for Proficiency Testing in Histology (Amy Porter) 7. Re: CAP Inspection question for Proficiency Testing in Histology (Rene J Buesa) 8. Pascal (Orr, Rebecca) 9. Ultra-Low Freezer Recommendation (Amber Tyler ANTYLER) 10. Salary in Texas (Reuel Cornelia) 11. Refurbished Equipment (Kelly Larson) 12. reply to Mouse Eyes (Lawrence D Lanberg/O/VCU) 13. Why tissue sections from resin blocks have black deposits? (Sulekha Ravi) 14. Alizarin red staining (pex) 15. Muscle ATPase: Barbital Substitute (Michele French) 16. Mouse eyes (Paula Pierce) ---------------------------------------------------------------------- Message: 1 Date: Thu, 17 Aug 2006 11:10:29 -0600 From: "Liz Chlipala" Subject: RE: [Histonet] Collagen testing To: "'Sebree Linda A.'" , "'Histonet'" Message-ID: <000001c6c220$0a735370$0300a8c0@domain.Premier> Content-Type: text/plain; charset="US-ASCII" Linda We can run Collagen I, II, III, IV and VII for immunohistochemistry, We are currently working on Collagen X, we also can run aggrecan and then markers for dermis specimens such as talin, vitronectin, etc. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Sebree Linda A. Sent: Thursday, August 17, 2006 10:50 AM To: Histonet Subject: [Histonet] Collagen testing > Hello histonetters, > > A researcher at our institution is looking for a reference lab that > performs collagen testing by IHC. I know of Collagen Types I, II, III > & IV but don't know if there are any others. If there are reference > labs out there that perform more than just Type IV, please let me know > and I'll pass the information along. > > Thank you, > > Linda Sebree, HT(ASCP) > University of Wisconsin Hospital & Clinics > IHC/ISH Laboratory > A4/204-3224 > 600 Highland Ave. > Madison, WI 53792 > (608)265-6596 > FAX: (608)262-7174 > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet __________ NOD32 1.1712 (20060817) Information __________ This message was checked by NOD32 antivirus system. http://www.eset.com ------------------------------ Message: 2 Date: Thu, 17 Aug 2006 13:38:37 -0400 From: "Rice, Michael" Subject: [Histonet] purchasing refurbished equipment To: Message-ID: <3BC92F29BE821745AB15E04C98EE028D6D3964@HCH2KMAIL.holy-cross.com> Content-Type: text/plain; charset="us-ascii" The main idea, is to find and use a company that will guarantee its equipment, and will furnish a list of references. I have used Imeb on several occasions for refurbished items and have always been satisfied with their level of service. Michael Rice CT.HT(ASCP) Supervisor Of Pathology Holy Cross Hospital Ft Lauderdale, Fl 33308 954.776.3070 ====================== Confidentiality Notice: Prepared in Anticipation of Litigation. Attorney-Client Privileged. This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original. ====================== ------------------------------ Message: 3 Date: Fri, 18 Aug 2006 02:06:13 +0800 From: Shirley PHUA Subject: [Histonet] Shirley is away : 18 August 2006 (Friday) To: histonet Message-ID: Content-Type: text/plain; charset=US-ASCII I will be out of the office from 08/18/2006 to 08/18/2006. I will return on 21 August 2006 (Monday). Pathologists : I will process your requests when I return. Otherwise, if urgent, please forward your mail to henry_kyaw@hsa.gov.sg Regrets for the inconveniences caused. ------------------------------ Message: 4 Date: Thu, 17 Aug 2006 14:26:46 EDT From: Steven2146@aol.com Subject: [Histonet] Re: Histonet Digest, Vol 33, Issue 22 To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" HI... If you're looking for reliable refurbished histology equipment, Belair is the best out there....the machines not only are TOTALLY reconditioned, but when you receive them, they look brand new....Belair, as far as I know, is the only company out there selling refurbished equipment, that offers a one year, unconditional guarantee. Steven Lee HT ASCP ------------------------------ Message: 5 Date: Thu, 17 Aug 2006 14:13:14 -0500 From: "Sally Prouty" Subject: [Histonet] mouse eyes To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; format=flowed Does anyone have a protocol for processing mouse eyes that doesn't cause them to end up looking like raisins? Besides hand processing we haven't find a consistant method. Sally J. Prouty Research Assistant, Campbell Laboratory Howard Hughes Medical Institute Department of Physiology & Biophysics University of Iowa College of Medicine 4283 Carver Biomedical Research Building Iowa City, IA 52242-1101 _________________________________________________________________ Express yourself instantly with MSN Messenger! Download today - it's FREE! http://messenger.msn.click-url.com/go/onm00200471ave/direct/01/ ------------------------------ Message: 6 Date: Thu, 17 Aug 2006 15:23:21 -0400 From: "Amy Porter" Subject: [Histonet] CAP Inspection question for Proficiency Testing in Histology To: Message-ID: <002301c6c232$99d82330$8e7a0923@HistoJJ> Content-Type: text/plain; charset="iso-8859-1" Can anyone out there is histoland tell me how they are meeting the proficiency testing program requirements for CAP inspections specifically with regards to histology..... I have spoken with CAP about the HistoQIP program and they consider that an educational program, not a proficiency program. Just wondering what other options are out there if any. Thanks for your responses in advance! Amy S. Porter, HT(ASCP) QIHC - Supervisor Michigan State University Investigative HistoPathology Laboratory Department of Physiology / Division of Human Pathology 2100 Biomedical Physical Sciences Bldg. Room #2133 East Lansing, MI 48824-3320 Phone: (517) 355-6475 ext 1480 / Fax: (517) 432-1368 Email: portera@msu.edu www.humanpathology.msu.edu ------------------------------ Message: 7 Date: Thu, 17 Aug 2006 12:40:46 -0700 (PDT) From: Rene J Buesa Subject: Re: [Histonet] CAP Inspection question for Proficiency Testing in Histology To: Amy Porter , histonet@pathology.swmed.edu Message-ID: <20060817194046.17625.qmail@web61216.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Amy: I used to have "competency check lists" and "standard of performance descriptions" for each mayor task. Along the year I used to observe the activity of each HT in each task and evaluated them against those lists. The deficiencies I found were documented and used for the individual PI of each HT, which went to their personal file. When during the CAP inspections I was asked about this aspect I showed them the PI developed for each HT that required a re-training on any given task. They were pleased with this approach to documenting the proficiency in completing histology tasks. Hope this will help you! Ren? J. Amy Porter wrote: Can anyone out there is histoland tell me how they are meeting the proficiency testing program requirements for CAP inspections specifically with regards to histology..... I have spoken with CAP about the HistoQIP program and they consider that an educational program, not a proficiency program. Just wondering what other options are out there if any. Thanks for your responses in advance! Amy S. Porter, HT(ASCP) QIHC - Supervisor Michigan State University Investigative HistoPathology Laboratory Department of Physiology / Division of Human Pathology 2100 Biomedical Physical Sciences Bldg. Room #2133 East Lansing, MI 48824-3320 Phone: (517) 355-6475 ext 1480 / Fax: (517) 432-1368 Email: portera@msu.edu www.humanpathology.msu.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail Beta. ------------------------------ Message: 8 Date: Thu, 17 Aug 2006 15:01:09 -0500 From: "Orr, Rebecca" Subject: [Histonet] Pascal To: Message-ID: Content-Type: text/plain; charset="us-ascii" We use the Biocare Decloaker in our lab. It's the same instrument as the Pascal. We use Dako ER/PR on our lab. We can run 4 tissue tek slide rack/containers at a time for a total of 98 slides in one run. Hope this helps. Becky Orr, CLA, HT(ASCP) QIHC Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2763 Hello All, I was wondering if anyone can tell me if they are using Pascal (from Dako) for ER/PR? If so, how do you like it? Does it take longer than a steamer for antigen retrieval? What do you think of it? Any information you can provide would be great! Thanks!! Jessica Piche-Grocki, HT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ ***************** ------------------------------ Message: 9 Date: Thu, 17 Aug 2006 16:28:59 -0400 From: Amber Tyler ANTYLER Subject: [Histonet] Ultra-Low Freezer Recommendation To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Hello, Has anyone had any experience with ultra-low freezers by Sanyo. I already know about rev-co, but I found a comparable freezer by sanyo that is a bit cheaper. Thank you, Amber Amber N. Tyler, Ph.D. Research Scientist Dept of Psychology (PO Box 26170) 296 Eberhart Building, Walker Avenue University of North Carolina - Greensboro Greensboro, NC 27402 ------------------------------ Message: 10 Date: Thu, 17 Aug 2006 16:44:10 -0500 From: "Reuel Cornelia" Subject: [Histonet] Salary in Texas To: Message-ID: Content-Type: text/plain; charset=US-ASCII Just wanted to hear some feedback on latest Wage data for Histotech supervisors particularly in dallas,texas. I want specifics, either per hour or salaried.Thank you. ******************************************************************************************************************* Texas Scottish Rite Hospital for Children is one of the nation's leading pediatric centers for the treatment of orthopedic conditions, certain related neurological disorders and learning disorders, such as dyslexia This email transmission and/or its attachments may contain confidential health information, intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing it to any other party unless required to do so by law and is required to delete/destroy the information after its stated need has been fulfilled. If you are not the intended recipient, any disclosure, copying, distribution or action taken in reliance on the contents of this email transmission is prohibited. If you have received this information in error, please notify the sender immediately and delete this information. We appreciate your efforts to protect the children's confidential information. ******************************************************************************************************************* ------------------------------ Message: 11 Date: Thu, 17 Aug 2006 18:02:22 -0400 From: "Kelly Larson" Subject: [Histonet] Refurbished Equipment To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; format="flowed" We purchased a used Tissue-Tek processor from Rankin. It had some problems to start with but they stood by their product and fixed it for us free of charge. They seem to be pretty honest people to work with. ------------------------------ Message: 12 Date: Thu, 17 Aug 2006 23:18:09 -0400 From: Lawrence D Lanberg/O/VCU Subject: [Histonet] reply to Mouse Eyes To: prouty27 <%t@vcu.edu> msn.com Cc: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="ISO-8859-1" Dear Ms. Prouty, I do mouse eyes -- ev looking like raisins, do you me wrinkle???? Or are you simply referring th Osmium Tetroxide imparts? I am always developing our protocols, but my ultra-thin sections come out more specifi Larry Lanberg PO Sanger Hall 2-032 Richmond, VA&n 804.828.7974 804.648.3246 ------------------------------ Message: 13 Date: Fri, 18 Aug 2006 10:19:30 +0100 (BST) From: Sulekha Ravi Subject: [Histonet] Why tissue sections from resin blocks have black deposits? To: histonet@lists.utsouthwestern.edu Message-ID: <20060818091930.79205.qmail@web25009.mail.ukl.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Dear members I have a problem with my resin blocks.We make resin blocks for non decalcified bone and vessel with metallic stent in my research laboratory. We dehydrate the tissue in ascending grades of alcohol, impregnate with methyl methacrylate monomer and then make blocks in polymerised methyl methacrylate.Some areas of tissue in these blocks have white opaque precipitate like dirt in it. When sections from such blocks are seen under microscope, these areas are black precipitate like deposits, masking the entire cellular architecture. We tried sonicating the sections in water with and without detergents. But nothing helped. Please help me to rectify this serious problem. Thanks Sulekha. Sr. Technical Asst SCTIMST Trivandrum Kerala India 695012 --------------------------------- Copy addresses and emails from any email account to Yahoo! Mail - quick, easy and free. Do it now... ------------------------------ Message: 14 Date: Fri, 18 Aug 2006 18:55:32 +0800 (CST) From: pex Subject: [Histonet] Alizarin red staining To: Histonet@lists.utsouthwestern.edu Message-ID: <20060818105532.40790.qmail@web15212.mail.cnb.yahoo.com> Content-Type: text/plain; charset=gb2312 Hello, I would like to perform Alizarin Red staining for mineralization nodules. Can someone do me a favor to show your protocol about it ? Thank you. Guofeng --------------------------------- ????????????-3.5G??????20M???? ------------------------------ Message: 15 Date: Fri, 18 Aug 2006 09:50:35 -0400 From: Michele French Subject: [Histonet] Muscle ATPase: Barbital Substitute To: histonet@lists.utsouthwestern.edu Message-ID: <44E5C5AB.4070702@bms.com> Content-Type: text/plain; format=flowed; charset=ISO-8859-1 Good Morning Everyone! I searched the archives and saw a few messages back in 2004 about using Glycine as a substitute for Sodium Barbital in the ATPase Stain for muscle fiber typing. The original e-mail did not include a recipe for the replacement of the barbital acetate preincubation solutions. It only talked about one preincubation solution at pH 9.4. I was wondering if anyone currently uses this substitute successfully and could send me your procedure? Thanks! Michele French Bristol-Myers Squibb Lawreceville, NJ ------------------------------ Message: 16 Date: Fri, 18 Aug 2006 07:49:57 -0700 (PDT) From: Paula Pierce Subject: [Histonet] Mouse eyes To: histonet@lists.utsouthwestern.edu Message-ID: <20060818144958.28339.qmail@web50107.mail.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 I process to paraffin and cut whole eyes from everything from drosophila to ostrich with no retinal detachment or shattered lenses. How are you processing now? Paula Pierce, HTL(ASCP)HT Excalibur Pathology, Inc. 630 N. Broadway Moore, OK 73160 405-759-3953 contact@excaliburpathology.com www.excaliburpathology.com ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 33, Issue 23 **************************************** ________________________________________________________________________ Check out AOL.com today. Breaking news, video search, pictures, email and IM. All on demand. Always Free. From tfranzod <@t> dal.ca Fri Aug 18 12:33:01 2006 From: tfranzod <@t> dal.ca (Tamara Franz-Odendaal) Date: Fri Aug 18 12:33:34 2006 Subject: [Histonet] Alizarin Red staining Message-ID: <011401c6c2ec$5e1458e0$7220ad81@tam> I routinely do Alizarin red staining for bone in embryos. The protocol is very simple. Some steps are only required if you need to bleach pigment (skin or eye pigment) or digest surrounding tissue from your specimens. Essentially, after fixing, place samples in water overnight, then into saturated tetraborate (Borax) for 12 hours, then into 1% KOH with alizarin red (1mg/ml) overnight, then water to rinse out excess staining (30min). Then into a graded series of 1% KOH with glycerol (80:20), (50:50), (20:80) until pure glycerol. If you need to bleach or digest tissue you need to add a hydrogen peroxide and trypsin step. I presume you are doing you staining on whole tissue pieces and not sections. Tamara Tamara Franz-Odendaal, PhD. Post-doctoral Researcher Biology Dept., Dalhousie University Halifax, NS, B3H 4J1 Canada 902-494 3335 From GoodwinD <@t> pahosp.com Fri Aug 18 15:19:39 2006 From: GoodwinD <@t> pahosp.com (Goodwin, Diana) Date: Fri Aug 18 15:19:51 2006 Subject: [Histonet] anti-human Nanog Message-ID: <80CDD9C3FEEAFD4982B114C4A6DFD00E02CB808D@uphsmbx2.UPHS.PENNHEALTH.PRV> Greetings. I am interested in hearing from anyone who has had success with this Ab -- vendor, dilution, + control tissue, detection kit. Specifically interested in anyone using on the Ventana Benchmark XT, but please submit other system success stories. Thanks, Diana Goodwin Supervisor, Anatomic Pathology Pennsylvania Hospital Preston 655-C ph. 215-829-6532 pager 215-422-5160 fax 215-829-7564 e-mail goodwind@[pahosp.com The information contained in this e-mail message is intended only for the personal and confidential use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this document in error and that any review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by e-mail, and delete the original message. From dgalos <@t> nmsu.edu Fri Aug 18 15:47:15 2006 From: dgalos <@t> nmsu.edu (Dylan Galos) Date: Fri Aug 18 15:47:22 2006 Subject: [Histonet] Question About Cryosectioning Message-ID: Hello - My name is Dylan Galos, and I am using a cryostat to do cross section slides of a fish at 40 microns thick. The fish has not been fixed yet, and was embedded in a water-based media. I am cutting at 40 microns thickness and have difficulty getting the bone tissue surrounding the spinal cord to stick to my slides. Please email me at dgalos@nmsu.edu if you have any sugestions or advice. Thank you. From katherine-walters <@t> uiowa.edu Fri Aug 18 16:48:33 2006 From: katherine-walters <@t> uiowa.edu (Walters, Katherine S) Date: Fri Aug 18 16:48:36 2006 Subject: [Histonet] nerve tissue in bone Message-ID: Dear bone experts: What is the best method to stain nerves in bone tissue? Thanks, Kathy From mbmphoto <@t> gmail.com Sun Aug 20 15:41:22 2006 From: mbmphoto <@t> gmail.com (Maria Mejia) Date: Sun Aug 20 15:40:24 2006 Subject: [Histonet] super mega tissue cassettes 72mmx52mmx17mm Message-ID: <60D526CD-D55C-4EA3-8091-07E455DE4360@gmail.com> Our research lab is seriously thinking about using these super mega tissue cassettes that measure 72mmx52mmx17mm in size!!! However, before we do buy & use them - I'd like to get further information such as obtaining a working tissue processing protocol for large brain blocks!!! 1. Is there anyone out there that is successfully using these cassettes? Please contact me to provide further information. 2. I found that Surgipath is 1 of 2 vendors that carry these big giants & they also carry the metal embedding molds that go w/cassettes. However I need to know if there is a vendor that provides the tissue chuck adapter that firmly holds these cassettes & if it's something we can fit into our Reigert Jung rotary microtome. Vendors please contact me!!! Any information anyone can provide will be greatly appreciated. Yours Maria Bartola Mejia University of California San Francisco Department of Neurosurgery San Francisco, CA 94103 Phone: (415)-514-2954 (lab) From AnthonyH <@t> chw.edu.au Sun Aug 20 18:10:39 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Aug 20 18:12:01 2006 Subject: [Histonet] Alizarin red staining Message-ID: The following works well: Alizarin Red S for Calcium This technique allows the direct demonstration of insoluble calcium = cations where Alizarin Red S forms an insoluble orange red lake. The = technique is made specific by pH control (Garvey et al 1989). Alizarin Red S also forms lakes with other metals such as barium, = aluminium, mercury and magnesium. Fortunately, the bright red = colouration of calcium can be easily differentiated from the dark red of = other metal-lakes. Fixation: 10% buffered formalin. Microtomy: 5=A6=CCm paraffin sections. Solutions: 1. 1% aqueous Alizarin Red S (CI 58005) adjust pH (to 4.2) with 10% = ammonium hydroxide. 2. Absolute Isopropanol 3. 60% Isopropanol 4. Xylene Controls: Tissue containing calcium. Negative Control - place for 20 minutes in citrate buffer pH 3-4 and = wash well before staining. Technique: 1. Deparrafinise sections and hydrate. 2. Rinse slides in distilled water. 3. Place in Alizarin Red S solution 1-5 minutes, controlling with the = light microscope until the deposits are orange-red in colour but not = diffuse. 4. Rinse in 60% Isopropanol, 30 seconds. 5. Rinse in absolute isopropanol, 30 seconds. 6. Rinse in xylene and coverslip. Results: Calcium - orange red. =20 The calcium deposits stained with Alizarin Red S are also birefringent. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu = [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of pex Sent: Friday, 18 August 2006 8:56 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Alizarin red staining=20 Hello, =20 I would like to perform Alizarin Red staining for mineralization = nodules. Can someone do me a favor to show your protocol about it ? =20 Thank you. =20 Guofeng=20 =09 --------------------------------- = =D1=C5=BB=A2=C3=E2=B7=D1=D3=CA=CF=E4-3.5G=C8=DD=C1=BF=A3=AC20M=B8=BD=BC=FE= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu = http://lists.utsouthwestern.edu/mailman/listinfo/histonet From AnthonyH <@t> chw.edu.au Sun Aug 20 18:14:40 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Aug 20 18:15:57 2006 Subject: [Histonet] Muscle ATPase: Barbital Substitute Message-ID: Here is our method: Adenosine Triphosphatase (ATPases) Use Demonstration of muscle fibre types. Underlying Principle The principle relies on the ability of the enzyme to remove the terminal phosphate from the ATP, which then combines with calcium in the incubation solution to form an insoluble calcium phosphate. Cobalt is then exchanged for the calcium, which, after reaction with ammonium sulphide, forms a black, insoluble cobalt sulphide at the site of enzyme activity. Fixation and Sectioning Air dried unfixed 8?m cryostat sections Reagents 1. 1% calcium chloride Calcium chloride 5.0 g Distilled water 500ml 2. 2% Cobalt chloride Warning: Suspected Carcinogen - see MSDS Cobalt chloride 10.0 g Distilled water 500 ml 3. 1% Ammonium sulphide Warning: Flammable liquid, Irritant, Toxic stench - see MSDS 20% ammonium sulphide 0.5 ml Distilled water 9.5 ml 4. Acid Pre-incubation medium 0.2M Sodium Acetate Sodium Acetate Anhydrous 0.82 g Distilled water 50 ml 0.2M Acetic Acid Glacial Acetic Acid 0.6 ml Distilled water 50 ml 0.2M Acetate Buffer: pH 4.3 pH 4.6 0.2M Sodium Acetate 11 ml 18 ml 0.2M Acetic Acid 12 ml 13 ml Adjust to pH 4.3 or 4.6 with 0.2M Sodium Acetate or 0.2M Acetic Acid 5. 7.5% Calcium Chloride Calcium Chloride 7.5g Distilled water 100ml 6. 7.05% Glycine Glycine 7.05g Distilled water 100ml Freeze in 2ml aliquots, defrost one aliquot before use. 7. 5.625% Sodium Chloride Sodium Chloride 5.625g Distilled water 100ml 8. 3.5% Sodim Hydroxide Sodium Hydroxide 3.5g Distilled water 100ml 9. Alkaline Stock Warning: Irritant - see MSDS 7.05% Glycine 2ml 7.5% Calcium Chloride 2ml 5.625% Sodium Chloride 2ml 3.5% Sodium Hydroxide 2ml Distilled water 42ml 10. Incubating Medium Alkaline Stock 25 ml ATP (Sigma A-7699) 40 mg Adjust to pH 9.4 - 9.5 with 0.1M HCl Method for preparing 9.4 Substrate Solution 1. Prepare fresh Alkaline Stock for each run 2. Place in 37oC Oven for 20minutes 3. pH to 11 using 0.1M NaOH (to activate the ATP) 4. Add ATP 5. pH to 9.4 using 0.1M HCl Staining Method 1. Place one slide in 4.3 and one in 4.6 buffer at room temperature for 20 minutes 2. Wash each in distilled water 3 times 3. Place these slides (one from the 4.3 solution , one from 4.6 solution and the 9.4 slide) in the 9.4 substrate at 37?C for pH 9.4 10 mins pH 4.6 30 mins pH 4.3 45 mins 4. Place slides in 2 changes of 1% calcium chloride 3 min each 5. Place slides in 2% cobalt chloride 3 min 6. Wash well in distilled water 7. In fume cupboard drain slides well and place in ammonium sulphide solution for 1 min 8. Wash well in tap water 9. Dehydrate clear and mount. Results pH 9.4 Type 1 fibres pale Type 2A fibres intermediate Type 2B fibres dark pH 4.3 and pH 4.6 pH 4.3 pH 4.6 Type 1 fibres dark dark Type 2A fibres pale pale Type 2B fibres pale intermediate Type 2C fibres intermediate dark Notes This is a complicated stain and there are several areas in which one needs to be careful in order to achieve a good fibre type differentiation. 1. The pH of all solutions is critical 2. Timing is crucial 3. The stock ammonium sulfide must still be yellow. As it ages or oxidizes, it becomes more red and cannot be used. 4. The pH of all solutions must be adjusted at the temperature they will be used. References 1. Loughlin, M. (1993). Muscle biopsy. A laboratory investigation. Butterworth-Heinemann pp.78-79. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Michele French Sent: Friday, 18 August 2006 11:51 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Muscle ATPase: Barbital Substitute Good Morning Everyone! I searched the archives and saw a few messages back in 2004 about using Glycine as a substitute for Sodium Barbital in the ATPase Stain for muscle fiber typing. The original e-mail did not include a recipe for the replacement of the barbital acetate preincubation solutions. It only talked about one preincubation solution at pH 9.4. I was wondering if anyone currently uses this substitute successfully and could send me your procedure? Thanks! Michele French Bristol-Myers Squibb Lawreceville, NJ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From histo007 <@t> hotmail.com Mon Aug 21 06:44:54 2006 From: histo007 <@t> hotmail.com (Jim Ball) Date: Mon Aug 21 06:45:00 2006 Subject: [Histonet] co path Message-ID: The Facility I am presently working with has the Co path + operating system. I am total ignorant of the system, but I am sure there must be a procedure for sorting out special stains. The Techs are presently going through the entire Log and listing the special stains manually. The entire procedure can take up ward to 45 minutes for an experienced Tech and much longer for a first timer. Hopefully some one out there can suggest a procedure for pulling out only the special stains on to their own log, and hopefully a means of logging each special stain on their own log so separate work sheets could become a thing of the past. The path department has already approached the LIS department, but there seems to be some sort of language barrier present Java vs simple English. From coleman_manufacturing <@t> yahoo.com Mon Aug 21 08:03:25 2006 From: coleman_manufacturing <@t> yahoo.com (Coleman Manufacturing) Date: Mon Aug 21 08:03:36 2006 Subject: [Histonet] Coleman / Fisher Scientific Products Message-ID: <20060821130325.65580.qmail@web37604.mail.mud.yahoo.com> Let me first say I hope this message does not make anyone mad, for I know HistoNet was intended for educational and information sharing purposes only. With this said, I am Justin Coleman with Coleman Manufacturing out of South Carolina. Just wanted to post a message to everyone informing all that CMD is now a direct distributor for Fisher Scientific. We distribute any and all products from Fisher to a number of labratories currently. For example, we distribute laboratory equipment (Microwaves, cryostats, etc.), laboratory reagents, special stains, and disposables. Please do not hesitate to contact us if we can help you out; maybe we can do better on pricing for you! Thanks to all, Justin Coleman Coleman Manufacturing & Design South Carolina 803.633.2124 office 803.635-9401 fax From laurie.colbert <@t> huntingtonhospital.com Mon Aug 21 10:13:50 2006 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Mon Aug 21 10:13:57 2006 Subject: [Histonet] Meditech LIS Message-ID: <0BE6ADFAE4E7E04496BF21ABD346628006307D04@EXCHANGE1.huntingtonhospital.com> Can someone that uses Meditech in Pathology give me some feedback on the system. To what capacity do you use it in Pathology/Histology? Do you use it to accession specimens in the grossing room? Do you use it to print up a worklist at the end of the day that the histotechs will use for embedding and cutting? Do you print cassettes, slides, and/or slide labels using the system? Can you pull statistics out? Although we are getting Meditech in Pathology, I have been told that we will not be getting it in the grossing room, because we would have to pull up the histories when we are accessioning. Right now we use a typewriter to log everything in. I have worked with several LIS systems before, and we were able to just enter very basic info in order to accession the specimens. I would appreciate any info that you can give me - thanks! Laurie Colbert Huntington Hospital Pasadena, CA From David.Muskett <@t> RLC.NHS.UK Mon Aug 21 10:22:51 2006 From: David.Muskett <@t> RLC.NHS.UK (Muskett David) Date: Mon Aug 21 10:22:57 2006 Subject: [Histonet] Meditech LIS Message-ID: Hello We use Meditech Meditech has the advantage over other pathology systems that it allows electronic requisitioning if it is being used on the hospital wards. It is not well designed for histology and presents a number of practical difficulties using it. There is no proper word processing package The search functions are flawed We print slide labels We can print wordlists but not as good as other systems such as Telepath We do basic stats Once a specimen is accessed onto the system you can link to other pathologies - it is generally quite cumbersome. My advice is to spend a day with someone using it. Regards David David Muskett Chief Biomedical Scientist, Histology Royal Liverpool Children's NHS Trust Eaton Road Liverpool L12 2AP Telephone (0151) 293 3656 Fax (0151) 293 3617 www.alderhey.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: 21 August 2006 16:14 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Meditech LIS Can someone that uses Meditech in Pathology give me some feedback on the system. To what capacity do you use it in Pathology/Histology? Do you use it to accession specimens in the grossing room? Do you use it to print up a worklist at the end of the day that the histotechs will use for embedding and cutting? Do you print cassettes, slides, and/or slide labels using the system? Can you pull statistics out? Although we are getting Meditech in Pathology, I have been told that we will not be getting it in the grossing room, because we would have to pull up the histories when we are accessioning. Right now we use a typewriter to log everything in. I have worked with several LIS systems before, and we were able to just enter very basic info in order to accession the specimens. I would appreciate any info that you can give me - thanks! Laurie Colbert Huntington Hospital Pasadena, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Kari.Zajic <@t> HCAhealthcare.com Mon Aug 21 10:23:41 2006 From: Kari.Zajic <@t> HCAhealthcare.com (Zajic Kari) Date: Mon Aug 21 10:23:46 2006 Subject: [Histonet] Meditech LIS In-Reply-To: <0BE6ADFAE4E7E04496BF21ABD346628006307D04@EXCHANGE1.huntingtonhospital.com> Message-ID: <095327C7CDBDF64B9E9728A54799091E015CC610@ORLEV03.hca.corpad.net> Oh My Gosh a typewriter???? you poor thing!!! I use Meditech and you can do everything you are asking about! The only thing is I am not sure of slides or cassettes because I do not have that equiptment, but I can print labels. I have been working with Meditech for about 12 years now, you are welcome to respond to me personally and I'll try and help you the best I can! Kari Marie Zajic HTL,MLT Histology Supervisor Palms West Hospital Pathology Department 13001 State Road Eighty Loxahatchee, Florida 33470 phone: (561)798-6036 telefax: (561)753-4298 voicemail: (561)753-4299 pager: (561)610-4949 email: Kari.Zajic@HCAHealthcare.com This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Laurie Colbert Sent: Monday, August 21, 2006 11:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Meditech LIS Can someone that uses Meditech in Pathology give me some feedback on the system. To what capacity do you use it in Pathology/Histology? Do you use it to accession specimens in the grossing room? Do you use it to print up a worklist at the end of the day that the histotechs will use for embedding and cutting? Do you print cassettes, slides, and/or slide labels using the system? Can you pull statistics out? Although we are getting Meditech in Pathology, I have been told that we will not be getting it in the grossing room, because we would have to pull up the histories when we are accessioning. Right now we use a typewriter to log everything in. I have worked with several LIS systems before, and we were able to just enter very basic info in order to accession the specimens. I would appreciate any info that you can give me - thanks! Laurie Colbert Huntington Hospital Pasadena, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Lynne.Bell <@t> hitchcock.org Mon Aug 21 12:22:27 2006 From: Lynne.Bell <@t> hitchcock.org (Bell, Lynne) Date: Mon Aug 21 12:22:37 2006 Subject: [Histonet] Meditech LIS Message-ID: Laurie, We use Meditech here at our hospital. We use it in the grossing room to accession specimens, print specimen label, etc. We do not have to access the history of the patient to do this, however. We've been using it at our entire hospital for approximately 8 years, and in Pathology for about three years. It is relatively easy to use. We do use it for statistical reports, but we do not currently print a worksheet, but we do have the capability. If I can be of any help, please let me know. Lynne Bell, HT (ASCP) Lead Histologist Central Vermont Medical Center P.O. Box 547 Barre, VT 05641 802-371-4923 From Melissa.Gonzalez <@t> cellgenesys.com Mon Aug 21 12:30:21 2006 From: Melissa.Gonzalez <@t> cellgenesys.com (Melissa Gonzalez) Date: Mon Aug 21 12:30:37 2006 Subject: [Histonet] Fluorescent In Situ Hybridization Message-ID: Dear all, We are currently trying to obtain information on outsourcing samples for FISH. Does anyone know of contract labs that will perform this service? (Part. in Bay Area) Most places I have contacted no longer have anyone on staff able to perform hybridization. Any comments on easy of use with Invitrogen's FISH Tag kits? We are kind of limited on time, and are rather inexperienced in this area. Your input is greatly appreciated, Melissa Melissa A. Gonzalez R&D, Cell Genesys, Inc. South San Francisco, CA 94080 (650) 266-3168 From Kari.Zajic <@t> HCAhealthcare.com Mon Aug 21 12:40:33 2006 From: Kari.Zajic <@t> HCAhealthcare.com (Zajic Kari) Date: Mon Aug 21 12:40:38 2006 Subject: [Histonet] Fluorescent In Situ Hybridization In-Reply-To: Message-ID: <095327C7CDBDF64B9E9728A54799091E015CC613@ORLEV03.hca.corpad.net> I have limited experience as well but Genzyme Laboratories has 3 labs in CA and they do our FISH. 1-800-447-5816 Kari Marie Zajic HTL,MLT Histology Supervisor Palms West Hospital Pathology Department 13001 State Road Eighty Loxahatchee, Florida 33470 phone: (561)798-6036 telefax: (561)753-4298 voicemail: (561)753-4299 pager: (561)610-4949 email: Kari.Zajic@HCAHealthcare.com This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Melissa Gonzalez Sent: Monday, August 21, 2006 1:30 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fluorescent In Situ Hybridization Dear all, We are currently trying to obtain information on outsourcing samples for FISH. Does anyone know of contract labs that will perform this service? (Part. in Bay Area) Most places I have contacted no longer have anyone on staff able to perform hybridization. Any comments on easy of use with Invitrogen's FISH Tag kits? We are kind of limited on time, and are rather inexperienced in this area. Your input is greatly appreciated, Melissa Melissa A. Gonzalez R&D, Cell Genesys, Inc. South San Francisco, CA 94080 (650) 266-3168 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Mon Aug 21 12:40:59 2006 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon Aug 21 12:41:11 2006 Subject: [Histonet] Meditech LIS In-Reply-To: <0BE6ADFAE4E7E04496BF21ABD346628006307D04@EXCHANGE1.huntingtonhospital.com> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6D32@EMAIL.archildrens.org> We have Meditech. We do not have to enter a history to accession. We accession the specimens in Meditech. We print a worksheet every morning for slide check out. We do not use the worksheet for grossing because the specimens may not have been entered into Meditech yet at the time of grossing. (we still use a log book as well) We use a separate log for the grossing/embedding worksheet. We print all our slide labels in Meditech. We are on version 5.5. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert Sent: Monday, August 21, 2006 10:14 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Meditech LIS Can someone that uses Meditech in Pathology give me some feedback on the system. To what capacity do you use it in Pathology/Histology? Do you use it to accession specimens in the grossing room? Do you use it to print up a worklist at the end of the day that the histotechs will use for embedding and cutting? Do you print cassettes, slides, and/or slide labels using the system? Can you pull statistics out? Although we are getting Meditech in Pathology, I have been told that we will not be getting it in the grossing room, because we would have to pull up the histories when we are accessioning. Right now we use a typewriter to log everything in. I have worked with several LIS systems before, and we were able to just enter very basic info in order to accession the specimens. I would appreciate any info that you can give me - thanks! Laurie Colbert Huntington Hospital Pasadena, CA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From Lori.Disher <@t> HCAhealthcare.com Mon Aug 21 12:48:14 2006 From: Lori.Disher <@t> HCAhealthcare.com (Disher Lori) Date: Mon Aug 21 12:48:18 2006 Subject: [Histonet] Printing slide labels on Meditech Message-ID: <095327C7CDBDF64B9E9728A54799091E1A9823@ORLEV03.hca.corpad.net> Can someone who uses meditech in their Histo lab tell me if you can print slide labels from Meditech? Is there special software you need? We still hand write our labels and would like to find and easier way. Lori Disher Histology Lead Tech Fawcett Memorial Hospital * 941-627-6128 * 941-764-7071 * lori.disher@hcahealthcare.com From chopan24ac <@t> gmail.com Mon Aug 21 13:04:08 2006 From: chopan24ac <@t> gmail.com (cris aguilar) Date: Mon Aug 21 13:04:12 2006 Subject: [Histonet] Determination of hydroxyproline. Help me please Message-ID: <82d2ad480608211104y30fbe9bbl84b4315b201efbe8@mail.gmail.com> Hello everybody I am a medical student in Peru. At present, I have been developing my thesis in ventricular remodeling; therefore I need to quantify *"collagen content" in rat heart*, as fibrosis index. I have tried to quantify "collagen content by hydroxyproline determination" several times in my laboratory, but I failed. I have been following Reddy's technique: * * *A simplified method for analysis of Hydroxiproline in Biological tissues. * Reddy GK, Enwemeka CS. Clinical Biochemistry 1996;29:225-9. *My procedure: * * * *Preparation of myocardial samples: * The rats were weighed, anesthetized with sodium pentobarbital (50 mg/kg, intraperitoneal), and underwent median thoracotomy for heart removal. The left ventricle was separated and weighed. Later, the myocardial samples were "dehydrated" in pure alcohol for 24 hours (is this correct?). Finally, 50mg of wet tissue sample was manually homogenized (manualmente en un mortero) in 1mL NaCl. ** * * * Quantification of hydroxyproline* Duplicated Hyp standard (0, 2.0, 4.0, 6.0, 8.0, 10.0ml) and triplicated test samples (homogenate 10ml, 50mg wet tissue/ 1ml NaCl) were placed in high temperature polypropylene tubes of 2ml capacity. Later 50ml of 2N sodium hydroxide was added to each tube with Hyp standard and test samples and mixed at room temperature for 20 minutes. Samples were hydrolyzed (alkali hydrolysis in 2N NaOH) by fire (estufa) at 120?C for 20min. 50ml of Chloramine-T was added and mixed with the hydrolyzate and oxidation was allowed to proceed for 25 minutes at room temperature. 500ml of 1M Ehrlich's reagent was added to each sample and the samples were mixed and incubated at 65?C for 20 minutes. Finally the absorbance of samples was read at 550nm using a spectrophotometer. *1. **Is it necessary to dehydrate the sample for a later analysis? * * * *2. **Is alkaline hydrolysis (2N NaOH) the better way to liberate Hyp from samples? * * * *3. **Did the method use autoclave to hydrolyzate? I have been use a fire (estufa)?* *Is this correct?* * * *4. **How can I estimate Hydroxyproline content in rat heart (mg/g)?* * * Does anybody know how I can measure collagen content in rat heart? Has anyone done a Sirius red stain for collagen? If so, could I please have your protocol? *Help me please I am desperate, because I must present my thesis next October. * Thank you in advance. Cristian Aguilar Universidad Nacional de Trujillo From HornHV <@t> archildrens.org Mon Aug 21 13:16:09 2006 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon Aug 21 13:16:29 2006 Subject: [Histonet] Printing slide labels on Meditech In-Reply-To: <095327C7CDBDF64B9E9728A54799091E1A9823@ORLEV03.hca.corpad.net> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6D33@EMAIL.archildrens.org> We print our labels from Meditech. There is no software needed but your programmer will have to format the labels to your printer. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Disher Lori Sent: Monday, August 21, 2006 12:48 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Printing slide labels on Meditech Can someone who uses meditech in their Histo lab tell me if you can print slide labels from Meditech? Is there special software you need? We still hand write our labels and would like to find and easier way. Lori Disher Histology Lead Tech Fawcett Memorial Hospital * 941-627-6128 * 941-764-7071 * lori.disher@hcahealthcare.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From rjbuesa <@t> yahoo.com Mon Aug 21 13:41:29 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Mon Aug 21 13:41:40 2006 Subject: [Histonet] Fluorescent In Situ Hybridization In-Reply-To: Message-ID: <20060821184129.19920.qmail@web61218.mail.yahoo.com> Melissa: Before I started using the VYSIS/Abbot procedure, we used to send our HER-2 DNA to PhenoPath Laboratories in Seattle WA That was in 2002, I do not know if they still provide that service. Their telephone (206)374-9000 They were reliable. Hope this will help you. Ren? J. Melissa Gonzalez wrote: Dear all, We are currently trying to obtain information on outsourcing samples for FISH. Does anyone know of contract labs that will perform this service? (Part. in Bay Area) Most places I have contacted no longer have anyone on staff able to perform hybridization. Any comments on easy of use with Invitrogen's FISH Tag kits? We are kind of limited on time, and are rather inexperienced in this area. Your input is greatly appreciated, Melissa Melissa A. Gonzalez R&D, Cell Genesys, Inc. South San Francisco, CA 94080 (650) 266-3168 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Stay in the know. Pulse on the new Yahoo.com. Check it out. From Alice.Fallak <@t> uhsi.org Mon Aug 21 14:07:09 2006 From: Alice.Fallak <@t> uhsi.org (Fallak, Alice) Date: Mon Aug 21 14:07:17 2006 Subject: [Histonet] MICROWAVE PROCESSOR CASSETTE RACK Message-ID: WE NEED TO REPLACE OUR MICROWAVE PROCESSOR CASSETTE RACK. OUR INITIAL ONE WAS PURCHASED W/OUR PROCESSOR. THEY'RE TELLING ME IT WILL COST 461.00 FOR A REPLACEMENT!!!! HAS ANYONE FOUND ONE CHEAPER?? From gcallis <@t> montana.edu Mon Aug 21 14:11:25 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Mon Aug 21 14:11:36 2006 Subject: Outsourcing Re: [Histonet] Fluorescent In Situ Hybridization In-Reply-To: <20060821184129.19920.qmail@web61218.mail.yahoo.com> References: <20060821184129.19920.qmail@web61218.mail.yahoo.com> Message-ID: <6.0.0.22.1.20060821131029.01b519f8@gemini.msu.montana.edu> They also have a nice website - At 12:41 PM 8/21/2006, you wrote: >Melissa: > Before I started using the VYSIS/Abbot procedure, we used to send our > HER-2 DNA to PhenoPath Laboratories in Seattle WA That was in 2002, I do > not know if they still provide that service. > Their telephone (206)374-9000 They were reliable. > Hope this will help you. > Ren? J. > >Melissa Gonzalez wrote: > Dear all, > >We are currently trying to obtain information on outsourcing samples for >FISH. Does anyone know of contract labs that will perform this service? >(Part. in Bay Area) Most places I have contacted no longer have anyone >on staff able to perform hybridization. >Any comments on easy of use with Invitrogen's FISH Tag kits? >We are kind of limited on time, and are rather inexperienced in this >area. >Your input is greatly appreciated, >Melissa > > >Melissa A. Gonzalez >R&D, Cell Genesys, Inc. >South San Francisco, CA 94080 >(650) 266-3168 > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Stay in the know. Pulse on the new Yahoo.com. Check it out. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis HTL, HT, MT(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University Bozeman MT 59717 From gu.lang <@t> gmx.at Mon Aug 21 14:31:56 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Mon Aug 21 14:32:02 2006 Subject: [Histonet] polyacids in trichrome stains Message-ID: <000901c6c558$77133820$eeeea8c0@SERVER01> Hi! I've just read in a German textbook, that the polyacids (phosphormolbydenacid, phosphortungstenacid) render the connective tissue coarse to give the right staining in trichroms-methods. Is this really a correct explanation? Or only an old theory (the book isn't very old)? ;) Gudrun Lang From ploykasek <@t> phenopath.com Mon Aug 21 15:03:04 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Mon Aug 21 15:03:19 2006 Subject: [Histonet] Fluorescent In Situ Hybridization In-Reply-To: Message-ID: Hi Melissa, I work for PhenoPath Laboratories in Seattle, WA. We have extensive experience in FISH. We have a contract research department that could perform this type of work for you. Most of our experience is with Vysis probes - including Her2Neu, lymphoma translocations, and other solid tumor probes. If you would like more information, please contact me or Marilyn Skelly, the contract research manager. You can contact Marilyn at mskelly@phenopath.com or 206-374-9000. Thank you. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Dear all, > > We are currently trying to obtain information on outsourcing samples for > FISH. Does anyone know of contract labs that will perform this service? > (Part. in Bay Area) Most places I have contacted no longer have anyone > on staff able to perform hybridization. > Any comments on easy of use with Invitrogen's FISH Tag kits? > We are kind of limited on time, and are rather inexperienced in this > area. > Your input is greatly appreciated, > Melissa > > > Melissa A. Gonzalez > R&D, Cell Genesys, Inc. > South San Francisco, CA 94080 > (650) 266-3168 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From nfournier <@t> sasktel.net Mon Aug 21 15:06:59 2006 From: nfournier <@t> sasktel.net (N Fournier) Date: Mon Aug 21 15:07:04 2006 Subject: [Histonet] Encountering the same problem with wrinkled rat brain sections Message-ID: Hi Colleagues, I apologize first for the long email; however, I wanted to include as much information as possible. We are still having a problem on what I have posted previously on regarding wrinkling of rat brain sections after mounting. Our tissue was fixed with 4% paraformaldehyde for 48 hrs prior to being sectioned at 50 micron on a Vibratome 3000. The vibratome bath contains 0.1 M PB at 2 to 4 degrees C. Typically, we have processed the tissue for various immunohistochemistry free-floating protocols, such as BrdU, doublecortin, NeuN, GAD67, etc. We noticed that after the staining was complete we would encountered severe wrinkling when the tissue was brought through alcohols and stained with cresyl violet. We have altered a variety of parameters such as as our cresyl violet protocol, using gelcohol in mounting solution, using Fisher Superfrost/Plus versus gelatin subbed slides, etc. However, we found that the wrinkling was minimized, if after mounting the sections, they were throroughly dried (by using a Kim Wipe to wipe up residual 0.1 M PB. We use 0.1 M PB, pH=7.4 for mountng) and allowed to sit for at least 2-3 hrs (vertical position) before being placed in a small oven set to 38 to 40 degrees C for overnight. (I have also left tissue in the oven for at least 2 to 3 days before running the slides through alcohols, xylene and coverslipping with no observed differences in staining quality). None of the other changes were associated with any real differences in tissue wrinkling. We have also used non- immunohistochemistry processed tissue and have encountered the s ame wrinkling problems. I don't think my method for mounting is incorrect, but just to let you know, I typically fill a petri dish with 0.1 M PB and using a paint brush I gently float the sections on to a coverslip after which I lightly transfer the section to a dry slide (e.g., Fisher Superfrost/Plus) and gently absorb residual PB with a Kim Wipe. Now I thought the issue was the result of the sections not being dry enough prior to coverslipping; however, I have started to second guess this. A colleague of mine, who has far more experience than I do using vibrating microtomes, encountered the same problem. They were using 40-50 micron thick fixed rat brain sections through a free-floating procedure for immunofluorescence. After the staining was complete, the sections were mounted on to Fisher Superfrost/Plus slides and antifade medium was applied. Significant wrinkling was still observed. They have also tried mounting sections, drying them, and leaving them in the fridge overnight prior to coverslipping. Wrinkling was still observed. My colleague does not want to dry placing the slides in the drying oven because he feels this might effect the fluorescence. Finally, they have used a different vibrating microtome and the issues were not observed. We do not experience these problems with cryosection tissue and are now starting to wonder if the problem may be the result of using a vibrating microtome. Has anyone encountered similar problems using these types of sections and determined methods to fix this? Any help is appreciated, Thanks, Neil From dfvilleg <@t> mtu.edu Mon Aug 21 15:45:22 2006 From: dfvilleg <@t> mtu.edu (Diego Villegas) Date: Mon Aug 21 15:45:31 2006 Subject: [Histonet] collagen fiber and GAGs Message-ID: <44EA1B62.1060903@mtu.edu> Hi all, I am using Safranin O (GAGs) and Weigert's Resorcin-fuchsin solution (Collagen) in cow meniscal attachments samples. Could I combine these two procedures to look up collagen fibers and GAGs in the same sample?. Thanks, Diego Villegas Biomechanics Lab Michigan Tech University From mcauliff <@t> umdnj.edu Mon Aug 21 16:32:52 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Mon Aug 21 16:32:19 2006 Subject: [Histonet] polyacids in trichrome stains In-Reply-To: <000901c6c558$77133820$eeeea8c0@SERVER01> References: <000901c6c558$77133820$eeeea8c0@SERVER01> Message-ID: <44EA2684.80309@umdnj.edu> I seem to remember that Richard Horobin wrote a authoritative paper on this subject more than a few years ago. Also, John Kiernan's textbook (and website?) almost certainly offers an explanation. Geoff Gudrun Lang wrote: >Hi! >I've just read in a German textbook, that the polyacids >(phosphormolbydenacid, phosphortungstenacid) render the connective tissue >coarse to give the right staining in trichroms-methods. Is this really a >correct explanation? Or only an old theory (the book isn't very old)? ;) > >Gudrun Lang > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From jqb7 <@t> cdc.gov Mon Aug 21 18:05:45 2006 From: jqb7 <@t> cdc.gov (Bartlett, Jeanine (CDC/CCID/NCID)) Date: Mon Aug 21 18:05:50 2006 Subject: [Histonet] MICROWAVE PROCESSOR CASSETTE RACK References: Message-ID: What brand and model? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu on behalf of Fallak, Alice Sent: Mon 8/21/2006 3:07 PM To: Histonet (E-mail) Cc: Subject: [Histonet] MICROWAVE PROCESSOR CASSETTE RACK WE NEED TO REPLACE OUR MICROWAVE PROCESSOR CASSETTE RACK. OUR INITIAL ONE WAS PURCHASED W/OUR PROCESSOR. THEY'RE TELLING ME IT WILL COST 461.00 FOR A REPLACEMENT!!!! HAS ANYONE FOUND ONE CHEAPER?? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tahseen <@t> brain.net.pk Mon Aug 21 20:23:09 2006 From: tahseen <@t> brain.net.pk (Tahseen) Date: Mon Aug 21 20:32:28 2006 Subject: [Histonet] Evaluation of Nitric Oxide Synthase (e-NOS) Message-ID: <000101c6c58a$cee6d1b0$511e80cb@p> Dear bone experts: What is the best method to"Evaluation of Nitric Oxide Synthase (e-NOS) in chronically inflamed human dental pulp"? Thanks, Tahseen From kappeler <@t> patho.unibe.ch Tue Aug 22 01:03:12 2006 From: kappeler <@t> patho.unibe.ch (Andi Kappeler) Date: Tue Aug 22 01:03:47 2006 Subject: [Histonet] VEGF antibody, clone VG1 Message-ID: <004e01c6c5b0$b89c12f0$27955c82@patho.unibe.ch> Has anybody successfully used Dako's mouse-anti human VEGF, clone VG1? We have tried it at 2-4 ug/ml with 7 different pretreatments (enzymes, HIER with different buffers) on various tissues (including tonsil, colon, pancreas, breast carcinoma, endometrium) and the best we have got are a few isolated positive exocrine cells in the pancreas and the lumina of vessels that stain positive. This is in sharp contrast to what Turley et al. (J Pathol 186:313-18, 1998), who developped the antibody, describe. Visualization is with a conventional 3-step ABC-system. Anybody with better results with that particular clone? Pretreatment? Antibody concentration? Positve controls? Many thanks for your feedback. Andi Kappeler Institute of Pathology, University of Bern, Switzerland From kappeler <@t> patho.unibe.ch Tue Aug 22 01:10:59 2006 From: kappeler <@t> patho.unibe.ch (Andi Kappeler) Date: Tue Aug 22 01:11:34 2006 Subject: [Histonet] MUC4 antibody, clone 1G8 Message-ID: <004f01c6c5b1$cef1e380$27955c82@patho.unibe.ch> We have tried Zymed's mouse-anti-MUC4, clone 1G8, on a series of colon carcinoma at 1 ug/ml, 7 different pretreatments (enzymes, HIER with various buffers), visualization with a conventional 3-step ABC-system. After HIER we get positive staining of endothelial cells (which, according to the manufacurer, is normal), however - in contrast to what the datasheet says - we get no or almost no staining of epithelial cells / carcinoma cells. Has anybody been able to demonstrate MUC4 in colon carcinoma? Any suggestions for better positive controls (okay, the endothelia are positive, but not the tumors), pretreatment, antibody concentration? Many thanks! Andi Kappeler Institute of Pathology, University of Bern, Switzerland From benardsolomona <@t> yahoo.com Tue Aug 22 07:12:40 2006 From: benardsolomona <@t> yahoo.com (Solomon Adebayo) Date: Tue Aug 22 07:12:45 2006 Subject: [Histonet] Information on Postgraduate programmes needed Message-ID: <20060822121240.37138.qmail@web36102.mail.mud.yahoo.com> Hi, I need information of post-graduate programmes in Immunohistochemistry/cytogenetics. I have just secured approval to seek admission for MSc programme in histopathology. I wish to help set up new services in my institution after the study. Any information on institutions training post-graduate students shall be of help. Thank you all. Benard Solomon Pathology Department, Unilorin Teaching Hospital, Ilorin, Kwara State, Nigeria __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From dusko.trajkovic <@t> pfizer.com Tue Aug 22 08:50:20 2006 From: dusko.trajkovic <@t> pfizer.com (Trajkovic, Dusko) Date: Tue Aug 22 08:50:31 2006 Subject: [Histonet] Bright field and fluorescence staining Message-ID: <3AD0BD3142459B4E9B12CBEAFF2B89B2034F0129@lajamrexm01.amer.pfizer.com> Hallo everyone, I'm posting this for a colleague of mine. Can someone help her with the staining question??? Thanks Dusko Do you know any classical bright field staining, such as H&E or Geimsa (ideal to distinguish blood cells) but would not interfere with fluorescence? Ideally, we need to do the immunostaining, then bright field stain, and be able to look at both fluorescence signal and bright field signal. Thanks a lot! Jessie ---------------------------------------------------------------------- LEGAL NOTICE Unless expressly stated otherwise, this message is confidential and may be privileged. It is intended for the addressee(s) only. Access to this E-mail by anyone else is unauthorized. If you are not an addressee, any disclosure or copying of the contents of this E-mail or any action taken (or not taken) in reliance on it is unauthorized and may be unlawful. If you are not an addressee, please inform the sender immediately. From gcallis <@t> montana.edu Tue Aug 22 09:24:48 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Aug 22 09:24:52 2006 Subject: [Histonet] Bright field and fluorescence staining In-Reply-To: <3AD0BD3142459B4E9B12CBEAFF2B89B2034F0129@lajamrexm01.amer. pfizer.com> References: <3AD0BD3142459B4E9B12CBEAFF2B89B2034F0129@lajamrexm01.amer.pfizer.com> Message-ID: <6.0.0.22.1.20060822081114.01b4de30@gemini.msu.montana.edu> I suggest using an alkaline phosphatase immunostaining method and then DAKO's permanent red. Counterstain with JUST hematoxylin. Chris van der Loos taught me to underdevelop the permanent red so have very delicate fluorescence but you need to control the development of this chromogen (a very sensitive one for AP!) with a microscope. If you let it go to a darker red endpoint, you still have fluorescence but more diffuse, even though this works well for bright field. You can adjust for how to view in both modes. Vector red also fluorescences, and it too needs underdevelopment. We find the DAKO a bit more sensitive than the Vector red, but if the antigen/antibody complex is plentiful and strong, you could try either. Be sure to use Tween 20 in the buffer with Vector red, it cleans up things and makes the VR sharper. We use it with polymer kits to have biotin free IHC, but you can use PR with any AP IHC method. Poymer kits are extremely sensitive, so be careful about antibody dilutions, etc - look at kit instructions. We use the ImmunoVision aka Powervision now sold by VisionBiosystems and also trying the new Biocare polymer system for rodents. The contrast with hematoxylin is excellent and without interference with this stain but eosin can't be used since it fluoresces and will probably interfer with the PR fluorescence. At 07:50 AM 8/22/2006, you wrote: >Hallo everyone, > >I'm posting this for a colleague of mine. > >Can someone help her with the staining question??? > >Thanks > >Dusko > > > > > >Do you know any classical bright field staining, such as H&E or Geimsa >(ideal to distinguish blood cells) but would not interfere with >fluorescence? Ideally, we need to do the immunostaining, then bright >field stain, and be able to look at both fluorescence signal and bright >field signal. > > > >Thanks a lot! > > > >Jessie > > > >---------------------------------------------------------------------- >LEGAL NOTICE >Unless expressly stated otherwise, this message is confidential and may be >privileged. It is intended for the addressee(s) only. Access to this >E-mail by anyone else is unauthorized. If you are not an addressee, any >disclosure or copying of the contents of this E-mail or any action taken >(or not taken) in reliance on it is unauthorized and may be unlawful. If >you are not an addressee, please inform the sender immediately. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From studentofdeath <@t> hotmail.com Tue Aug 22 09:44:41 2006 From: studentofdeath <@t> hotmail.com (Amy Senn) Date: Tue Aug 22 09:44:53 2006 Subject: [Histonet] (no subject) Message-ID: Hello all, We changed vendors recently, and are now using Thermo's Formal Fix in plastic containers with red spigots. Does anyone in histoland have suggestions/advice for opening the plastic spout before screwing the spigot on? Everything we've tried just doesn't seem safe to get these damn containers open! Scalpels are working the best so far, but I'm afraid I'll seriously cut my fingers/hand when I'm pushing the blade into the rough plastic. We don't have anything else with a tip sharp enough to pierce the plastic---so we tried hitting a metal handle with a mallot, but the formalin splashes something awful when we do get through the container. Anything you could suggest would be appreciated! Thanks so much! Amy Quote of the Day: 'Procrastination is like masturbation. You only screw yourself.' "Vince" _________________________________________________________________ Windows Live Spaces is here! It’s easy to create your own personal Web site. http://spaces.live.com/signup.aspx From naje1972 <@t> yahoo.com Tue Aug 22 09:55:21 2006 From: naje1972 <@t> yahoo.com (cynthia haynes) Date: Tue Aug 22 09:55:27 2006 Subject: [Histonet] double staining Message-ID: <20060822145521.29882.qmail@web33011.mail.mud.yahoo.com> Good morning All, I have a question for all the histochemical stainers. I have 2 antibodies and they are Anti-Tau (3-repeat isoform RD3)and Anti-Tau (4-repeat isoform RD4).Both recognize Tau. Both are made in Mouse. Can I do double staining with these antibodies? If so which should I do first? Is it alright to use DAB and FAST RED(VECTOR) as the chromagens? Any suggestion will be greatly appreciated. Thank you in advance. Cynthia Haynes H.T. From lesley.bechtold <@t> jax.org Tue Aug 22 10:29:40 2006 From: lesley.bechtold <@t> jax.org (Lesley Bechtold) Date: Tue Aug 22 10:30:40 2006 Subject: [Histonet] Histology Technician Position available in Bar Harbor, Maine Message-ID: <20060822112940372.00000007076@spikey> Histology Technician I/II There is a regular, fulltime position in the Histology Service of Scientific Services at The Jackson Laboratory as a Histology Technician I/II. Responsibilities include laboratory maintenance, preparation of solutions, paraffin embedding, running the automated stainer and coverslipper and other routine histological procedures. Minimum qualifications include knowledge of basic biology and chemistry typically acquired through a high school diploma. Knowledge of mouse anatomy is preferred. Skill with basic laboratory equipment such as balances, pH meters and stir plates is required. Preference will be given to those candidates with some knowledge of histological techniques and the position will be filled at the Histology Technician I or Histology Technician II level, depending on skills and experience. This is a career-track position and the incumbent will have the opportunity to further their skills and knowledge and become a nationally certified Histotechnologist. Required computer skills include: use of email, the internet, word processing and spreadsheets. Good interpersonal skills are necessary and the incumbent must have the ability and willingness to function effectively in a team environment. The Jackson Laboratory is one of the world's foremost centers for mammalian genetics research. Located in Bar Harbor, Maine, the Lab is adjacent to Acadia National Park. Mountains, oceans, forests, lakes and trails are all within walking distance. If you're looking for a more natural environment, this could be the opportunity you've been searching for. Interested individuals should apply online at www.jax.org, checking off requisition #0545. Please submit cover letter and resume as one document. Lesley S. Bechtold Senior Manager, Histopathology & Microscopy Sciences The Jackson Laboratory 600 Main St. Bar Harbor, ME 04609 207-288-6322 From gu.lang <@t> gmx.at Tue Aug 22 10:31:43 2006 From: gu.lang <@t> gmx.at (Gudrun Lang) Date: Tue Aug 22 10:31:46 2006 Subject: AW: [Histonet] polyacids in trichrome stains In-Reply-To: <44EA2684.80309@umdnj.edu> Message-ID: <000001c6c600$1298a0d0$eeeea8c0@SERVER01> I know John Kiernan's book and the chapter about trichromes. But why has someone the idea that polyacids render the fibers coarse or rough? This explanation is yet given to students. I am in doubt about it and would like to disprove it with an experts' knowledge. Gudrun -----Urspr?ngliche Nachricht----- Von: Geoff McAuliffe [mailto:mcauliff@umdnj.edu] Gesendet: Montag, 21. August 2006 23:33 An: gu.lang@gmx.at Cc: Histonetliste (Histonetliste) Betreff: Re: [Histonet] polyacids in trichrome stains I seem to remember that Richard Horobin wrote a authoritative paper on this subject more than a few years ago. Also, John Kiernan's textbook (and website?) almost certainly offers an explanation. Geoff Gudrun Lang wrote: >Hi! >I've just read in a German textbook, that the polyacids >(phosphormolbydenacid, phosphortungstenacid) render the connective >tissue coarse to give the right staining in trichroms-methods. Is this >really a correct explanation? Or only an old theory (the book isn't >very old)? ;) > >Gudrun Lang > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From jason.burrill <@t> crl.com Tue Aug 22 10:43:10 2006 From: jason.burrill <@t> crl.com (jason.burrill@crl.com) Date: Tue Aug 22 10:43:41 2006 Subject: [Histonet] Jason Burrill/Corp/CRLUSA is out of the office. Message-ID: I will be out of the office starting 08/21/2006 and will not return until 08/23/2006. I will respond to your message when I return. If you need to speak to someone regarding Histology samples please contact the Histology Lab at 978-658-6000 X1229. From gcallis <@t> montana.edu Tue Aug 22 11:54:29 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Aug 22 11:54:34 2006 Subject: AW: [Histonet] polyacids in trichrome stains In-Reply-To: <000001c6c600$1298a0d0$eeeea8c0@SERVER01> References: <44EA2684.80309@umdnj.edu> <000001c6c600$1298a0d0$eeeea8c0@SERVER01> Message-ID: <6.0.0.22.1.20060822104829.01ba2d80@gemini.msu.montana.edu> Gudrun, I just refiled this publication - Puchtler H and Isler H. The effect of phosphomolybdic acid on the stainability of connective tissues by various dyes, J Histochem Cytochem July, 1958 - pp 265-270. It is an old publication, but interesting. I will try to send the pdf to you via private email Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From KatieW <@t> alleninstitute.org Tue Aug 22 13:05:44 2006 From: KatieW <@t> alleninstitute.org (Katie Wasell) Date: Tue Aug 22 13:05:50 2006 Subject: [Histonet] Fluorescent In Situ Hybridization Message-ID: If you are still looking for an organization to provide you with FISH samples, I would recommend that you contact the Allen Institute for Brain Science located in Seattle, WA. We have a high throughput in situ hybridization facility capable of processing nearly 1,000 slides per day. www.alleninstitute.org The institute has several ISH protocols including single and double labeled FISH. I would be happy to pass your number on to the appropriate individuals. Katie Glattfelder katiew@alleninstitute.org Research Associate Allen Institute for Brain Science -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Melissa Gonzalez Sent: Monday, August 21, 2006 10:30 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fluorescent In Situ Hybridization Dear all, We are currently trying to obtain information on outsourcing samples for FISH. Does anyone know of contract labs that will perform this service? (Part. in Bay Area) Most places I have contacted no longer have anyone on staff able to perform hybridization. Any comments on easy of use with Invitrogen's FISH Tag kits? We are kind of limited on time, and are rather inexperienced in this area. Your input is greatly appreciated, Melissa Melissa A. Gonzalez R&D, Cell Genesys, Inc. South San Francisco, CA 94080 (650) 266-3168 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Tue Aug 22 13:49:28 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Aug 22 13:50:02 2006 Subject: [Histonet] Patient slide send-out Message-ID: <44EB197802000077000017D3@hcnwgwds01.hh.chs> Our Anatomic Pathology Office is overwhelmed with requests from patients asking for their pathology slides to be sent to another medical institution (for second opinion, additional surgery or therapy, etc.). Is it legal to charge patients for this service? We don't have the personnel to handle these requests in a timely fashion and we can no longer afford to "eat" the shipping costs. Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax From JWEEMS <@t> sjha.org Tue Aug 22 13:54:12 2006 From: JWEEMS <@t> sjha.org (Weems, Joyce) Date: Tue Aug 22 13:54:28 2006 Subject: [Histonet] Patient slide send-out Message-ID: <1CD6831EB9B26D45B0A3EAA79F7EBD3202DEE747@sjhaexc02.sjha.org> We ask the patients for a credit card number for shipping costs. Joyce -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard Cartun Sent: Tuesday, August 22, 2006 2:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Patient slide send-out Our Anatomic Pathology Office is overwhelmed with requests from patients asking for their pathology slides to be sent to another medical institution (for second opinion, additional surgery or therapy, etc.). Is it legal to charge patients for this service? We don't have the personnel to handle these requests in a timely fashion and we can no longer afford to "eat" the shipping costs. Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice ** The information contained in this message may be privileged and is confidential information intended for the use of the addressee listed above. If you are neither the intended recipient nor the employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on the contents of this information is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. Saint Joseph's Health System, Inc. From rjbuesa <@t> yahoo.com Tue Aug 22 14:05:17 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Aug 22 14:05:21 2006 Subject: [Histonet] Patient slide send-out In-Reply-To: <44EB197802000077000017D3@hcnwgwds01.hh.chs> Message-ID: <20060822190517.96261.qmail@web61216.mail.yahoo.com> Richard: We used to send recuts and they were charged at our current rate. We also added shipping/handling costs. The bill was sent to the facility the slides were mailed to. Ren? J. Richard Cartun wrote: Our Anatomic Pathology Office is overwhelmed with requests from patients asking for their pathology slides to be sent to another medical institution (for second opinion, additional surgery or therapy, etc.). Is it legal to charge patients for this service? We don't have the personnel to handle these requests in a timely fashion and we can no longer afford to "eat" the shipping costs. Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Stay in the know. Pulse on the new Yahoo.com. Check it out. From Kari.Zajic <@t> HCAhealthcare.com Tue Aug 22 14:05:18 2006 From: Kari.Zajic <@t> HCAhealthcare.com (Zajic Kari) Date: Tue Aug 22 14:05:26 2006 Subject: [Histonet] Patient slide send-out In-Reply-To: <44EB197802000077000017D3@hcnwgwds01.hh.chs> Message-ID: <095327C7CDBDF64B9E9728A54799091E015CC621@ORLEV03.hca.corpad.net> Richard, you are not alone! We have also seen an increase in patient and physician requests for "send-outs". I am not sure of charging, but I have made a "send-out" policy for our department that all requests be put in writing (scripts for physicians)and faxed,inform the patient/office that it will at least be 48 hours before it can be sent out, they must provide a shipping service account (FEDEX, UPS) or physically pick up the slides. We have also been being charged for these consults by the other facility so we also inform them that if the insurance cannot be charged, they are responsible for the bill. It seems to work but we do run into some problems now and again. If they cannot provide an overnight shipping service, we will USPS mail them certified but that seems to take too long for their liking (here it's around a week). Not having enough staff to handle the sendouts is always a problem, hence the 48 hours..helps slightly. Kari :) Kari Marie Zajic HTL,MLT Histology Supervisor Palms West Hospital Pathology Department 13001 State Road Eighty Loxahatchee, Florida 33470 phone: (561)798-6036 telefax: (561)753-4298 voicemail: (561)753-4299 pager: (561)610-4949 email: Kari.Zajic@HCAHealthcare.com This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard Cartun Sent: Tuesday, August 22, 2006 2:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Patient slide send-out Our Anatomic Pathology Office is overwhelmed with requests from patients asking for their pathology slides to be sent to another medical institution (for second opinion, additional surgery or therapy, etc.). Is it legal to charge patients for this service? We don't have the personnel to handle these requests in a timely fashion and we can no longer afford to "eat" the shipping costs. Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cwscouten <@t> myneurolab.com Tue Aug 22 14:13:45 2006 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Tue Aug 22 14:13:42 2006 Subject: [Histonet] Encountering the same problem with wrinkled rat brainsections Message-ID: <5784D843593D874C93E9BADCB87342AB013074F1@tpiserver03.Coretech-holdings.com> Those of us associated with Vibratome company were concerned with your problem and have consulted an expert. His reply "The first thing I would suspect is the coating on their glass slides. Superfrost isn't good enough for adherence for free-floating sections at ~30 microns. Thaw-mounting from a cryostat onto Superfrost does work however, presumably because it is in the absence of immersion in buffer. But Niel also tried gelatin coating. The recipes and batches of this method are variable and oftentimes unreliable. I would suggest checking the recipe and protocol, using another recipe or simply using the poly-L-lysine technique: 1% poly-L-lysine (in water) smeared over a VERY CLEAN glass slide and allowed to dry for 5 minutes. Baking the tissue is unnecessary and probably a bad idea for ligand-binding as well as tissue adherence. Tissue is best allowed to dry naturally - without too much blotting or using fans, hair dryers, ovens, etc. Overnight at room temperature is fine but usually it really only needs 1-3 hours. Another possibility is that Neil's alcohols are in the wrong sequence or contaminated with water. They should be fresh, and tissue should be immersed for 3-5 minutes in this sequence: water, 50% EtOH, 75% EtOH, 90% EtOH, 100% EtOH, 100% EtOH again, and Xylene twice. The only way the vibratome is the culprit that I can see is if it is cutting too thick - way to thick - like 100 microns too thick. Pretty unlikely without being noticed. My money is on the slide coating - and save the oven for Thanksgiving!" Miles Cunningham, McLean Hospital Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of N Fournier Sent: Monday, August 21, 2006 3:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Encountering the same problem with wrinkled rat brainsections Hi Colleagues, I apologize first for the long email; however, I wanted to include as much information as possible. We are still having a problem on what I have posted previously on regarding wrinkling of rat brain sections after mounting. Our tissue was fixed with 4% paraformaldehyde for 48 hrs prior to being sectioned at 50 micron on a Vibratome 3000. The vibratome bath contains 0.1 M PB at 2 to 4 degrees C. Typically, we have processed the tissue for various immunohistochemistry free-floating protocols, such as BrdU, doublecortin, NeuN, GAD67, etc. We noticed that after the staining was complete we would encountered severe wrinkling when the tissue was brought through alcohols and stained with cresyl violet. We have altered a variety of parameters such as as our cresyl violet protocol, using gelcohol in mounting solution, using Fisher Superfrost/Plus versus gelatin subbed slides, etc. However, we found that the wrinkling was minimized, if after mounting the sections, they were throroughly dried (by using a Kim Wipe to wipe up residual 0.1 M PB. We use 0.1 M PB, pH=7.4 for mountng) and allowed to sit for at least 2-3 hrs (vertical position) before being placed in a small oven set to 38 to 40 degrees C for overnight. (I have also left tissue in the oven for at least 2 to 3 days before running the slides through alcohols, xylene and coverslipping with no observed differences in staining quality). None of the other changes were associated with any real differences in tissue wrinkling. We have also used non- immunohistochemistry processed tissue and have encountered the s ame wrinkling problems. I don't think my method for mounting is incorrect, but just to let you know, I typically fill a petri dish with 0.1 M PB and using a paint brush I gently float the sections on to a coverslip after which I lightly transfer the section to a dry slide (e.g., Fisher Superfrost/Plus) and gently absorb residual PB with a Kim Wipe. Now I thought the issue was the result of the sections not being dry enough prior to coverslipping; however, I have started to second guess this. A colleague of mine, who has far more experience than I do using vibrating microtomes, encountered the same problem. They were using 40-50 micron thick fixed rat brain sections through a free-floating procedure for immunofluorescence. After the staining was complete, the sections were mounted on to Fisher Superfrost/Plus slides and antifade medium was applied. Significant wrinkling was still observed. They have also tried mounting sections, drying them, and leaving them in the fridge overnight prior to coverslipping. Wrinkling was still observed. My colleague does not want to dry placing the slides in the drying oven because he feels this might effect the fluorescence. Finally, they have used a different vibrating microtome and the issues were not observed. We do not experience these problems with cryosection tissue and are now starting to wonder if the problem may be the result of using a vibrating microtome. Has anyone encountered similar problems using these types of sections and determined methods to fix this? Any help is appreciated, Thanks, Neil _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From barnhart717 <@t> comcast.net Tue Aug 22 14:27:43 2006 From: barnhart717 <@t> comcast.net (barnhart717@comcast.net) Date: Tue Aug 22 14:27:53 2006 Subject: [Histonet] Patient slide send-out Message-ID: <082220061927.25768.44EB5AAF000B9FE4000064A82200763692C9CEC99B9D0E08029D0E0D@comcast.net> When we receive a request to send blocks or slides to another facility for any reason we send the patients insurance information to the facility. In the letter we send we include the reason why the slides/blocks are being sent and to charge the patient's insurance not us. If they send us a bill we fight it. In the documentation that we keep I have different category for why the consult was done. Pathologist: is when our pathologist send out for a second opinion and we pay the cost. Physician, Patient or Treatment: These we will not pay for. The other facility eats the cost or charges the patient's insurance. We use priority mail because it usually arrives in about 3 days, we can track the shipment and it is a minimal cost. Becky Barnhart rbarnhart@summithealth.org -------------- Original message -------------- From: "Zajic Kari" > Richard, you are not alone! We have also seen an increase in patient and > physician requests for "send-outs". I am not sure of charging, but I have made a > "send-out" policy for our department that all requests be put in writing > (scripts for physicians)and faxed,inform the patient/office that it will at > least be 48 hours before it can be sent out, they must provide a shipping > service account (FEDEX, UPS) or physically pick up the slides. We have also been > being charged for these consults by the other facility so we also inform them > that if the insurance cannot be charged, they are responsible for the bill. It > seems to work but we do run into some problems now and again. If they cannot > provide an overnight shipping service, we will USPS mail them certified but that > seems to take too long for their liking (here it's around a week). > Not having enough staff to handle the sendouts is always a problem, hence the 48 > hours..helps slightly. > > Kari :) > > Kari Marie Zajic HTL,MLT > Histology Supervisor > Palms West Hospital > Pathology Department > 13001 State Road Eighty > Loxahatchee, Florida 33470 > phone: (561)798-6036 > telefax: (561)753-4298 > voicemail: (561)753-4299 > pager: (561)610-4949 > email: Kari.Zajic@HCAHealthcare.com > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL > information and may be read or used only by the intended recipient. If you are > not the intended recipient of the email or any of its attachment, please be > advised that you have received this email in error and that any use, > dissemination, distribution, forwarding, printing, or copying of this email or > any attached files is strictly prohibited. If you have received this email in > error, please immediately purge it and all attachments and notify the sender by > reply email or contact the sender at the number listed. > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard > Cartun > Sent: Tuesday, August 22, 2006 2:49 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Patient slide send-out > > > Our Anatomic Pathology Office is overwhelmed with requests from patients > asking for their pathology slides to be sent to another medical > institution (for second opinion, additional surgery or therapy, etc.). > Is it legal to charge patients for this service? We don't have the > personnel to handle these requests in a timely fashion and we can no > longer afford to "eat" the shipping costs. Thank you. > > Richard > > Richard W. Cartun, Ph.D. > Director, Immunopathology & Histology > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-0174 Fax > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From jdmd77 <@t> hotmail.com Tue Aug 22 17:58:07 2006 From: jdmd77 <@t> hotmail.com (Julia Dahl) Date: Tue Aug 22 17:58:15 2006 Subject: [Histonet] Patient slide send-out In-Reply-To: <082220061927.25768.44EB5AAF000B9FE4000064A82200763692C9CEC99B9D0E08029D0E0D@comcast.net> Message-ID: I agree that there are different categories of send-outs. As a consultant pathologist who is receiving the "send-out" - an 88321 (pathology second opinion on slides prepared elsewhere) is a professional component only CPT code and does not include any TC reimbursement (including shipping of slides and/or blocks). The cost of shipping, then, should be born by the party requesting the slides. If a pathologist is requesting a consultation, then their department pays for the shipping. If it is a patient, then it's the patient... what about a clinician requesting the send out? Do you want to be telling your clinicians that they will have to pay for shipping? It's an imporant and difficult question to answer. For BOTH sides - the originating pathology institution and the receiving pathology institution - second opinion pathology consultations are UNFORTUNATELY not economically feasible within the current CPT code and reimbursement schedules. Less than 60% of insurance companies actually will pay the consultant pathologist for the consultation. This is ridiculous given that the SAME insurance company will require a physician second opinion for patients with many medical conditions and surgeries PRIOR to paying for a surgical procedure or lengthy medical treatment... AND the insurance company does pay for that second opinion. As is evidenced by the literature and through anecdotal experience - second opinion in anatomic pathology is a valuable means to ensuring pathology accuracy. Nonetheless, this valuable service is neither convenient for the home institution nor the consultant... much less economically feasible for either institution. In the time that it takes for our organization to provide a consultation on an IBD with dysplasia or collagenous colitis with co-existent IBD (a sampling of today's consultations), our pathologists can sign out at least ten 88305 gastridites or adenomas. As an organization, do we dedicate our resources to primary diagnoses (more $$/less time) or to assisting our colleagues with their challenging cases (more time/less $$)? Something that should be separated from the current discussion is the instance of a send-out for a patient who is undergoing surgery at another institution. This is a quality assurance activity. Any institution in which a patient is undergoing DEFINITIVE therapy MUST review the pathologic material upon which the definitive therapy is rendered as a matter of ensuring the proper treatment and complete medical record for that patient within the treating institution. What would be the consequence of a sclerosing papilloma on biopsy called invasive carcinoma in Institution A, with the patient sent to Institution B for lumpectomy or mastectomy with sentinel node dissection? If Institution B requests the slides PRIOR to definitive therapy, and the diagnostic error is detected - the patient does not undergo an unnecessary surgical procedure and NONE of the physicians or medical institutions are sued. If Institution B fails to re-review the slides prior to definitive therapy, the patient has an unnecessary surgery with the resultant M&M - and EVERYONE (Institution A, Institution B, Pathologist A, Pathologist B and Surgeons) gets sued and will settle out of court. With this in mind - for second opinions prior to definitive therapy, the requesting institution must pay for the "consultation" if the patient's insurance will not. The down-side for the requesting institution is that in the absence of the insurance company paying for the "second opinion" - the hospital incurs the expense, and has no mechanism for recovering this expense as the DRG is not changed. Until this service is more equitably reimbursed - all parties are in a difficult situation. Julia Dahl MD >From: barnhart717@comcast.net >To: "Zajic Kari" ,"Richard Cartun" >, >Subject: RE: [Histonet] Patient slide send-out >Date: Tue, 22 Aug 2006 19:27:43 +0000 > >When we receive a request to send blocks or slides to another facility for >any reason we send the patients insurance information to the facility. In >the letter we send we include the reason why the slides/blocks are being >sent and to charge the patient's insurance not us. If they send us a bill >we fight it. In the documentation that we keep I have different category >for why the consult was done. Pathologist: is when our pathologist send >out for a second opinion and we pay the cost. Physician, Patient or >Treatment: These we will not pay for. The other facility eats the cost or >charges the patient's insurance. We use priority mail because it usually >arrives in about 3 days, we can track the shipment and it is a minimal >cost. > >Becky Barnhart >rbarnhart@summithealth.org > >-------------- Original message -------------- >From: "Zajic Kari" > > > Richard, you are not alone! We have also seen an increase in patient and > > physician requests for "send-outs". I am not sure of charging, but I >have made a > > "send-out" policy for our department that all requests be put in writing > > (scripts for physicians)and faxed,inform the patient/office that it will >at > > least be 48 hours before it can be sent out, they must provide a >shipping > > service account (FEDEX, UPS) or physically pick up the slides. We have >also been > > being charged for these consults by the other facility so we also inform >them > > that if the insurance cannot be charged, they are responsible for the >bill. It > > seems to work but we do run into some problems now and again. If they >cannot > > provide an overnight shipping service, we will USPS mail them certified >but that > > seems to take too long for their liking (here it's around a week). > > Not having enough staff to handle the sendouts is always a problem, >hence the 48 > > hours..helps slightly. > > > > Kari :) > > > > Kari Marie Zajic HTL,MLT > > Histology Supervisor > > Palms West Hospital > > Pathology Department > > 13001 State Road Eighty > > Loxahatchee, Florida 33470 > > phone: (561)798-6036 > > telefax: (561)753-4298 > > voicemail: (561)753-4299 > > pager: (561)610-4949 > > email: Kari.Zajic@HCAHealthcare.com > > > > This email and any files transmitted with it may contain PRIVILEGED or > > CONFIDENTIAL > > information and may be read or used only by the intended recipient. If >you are > > not the intended recipient of the email or any of its attachment, please >be > > advised that you have received this email in error and that any use, > > dissemination, distribution, forwarding, printing, or copying of this >email or > > any attached files is strictly prohibited. If you have received this >email in > > error, please immediately purge it and all attachments and notify the >sender by > > reply email or contact the sender at the number listed. > > > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard > > Cartun > > Sent: Tuesday, August 22, 2006 2:49 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Patient slide send-out > > > > > > Our Anatomic Pathology Office is overwhelmed with requests from patients > > asking for their pathology slides to be sent to another medical > > institution (for second opinion, additional surgery or therapy, etc.). > > Is it legal to charge patients for this service? We don't have the > > personnel to handle these requests in a timely fashion and we can no > > longer afford to "eat" the shipping costs. Thank you. > > > > Richard > > > > Richard W. Cartun, Ph.D. > > Director, Immunopathology & Histology > > Assistant Director, Anatomic Pathology > > Hartford Hospital > > 80 Seymour Street > > Hartford, CT 06102 > > (860) 545-1596 > > (860) 545-0174 Fax > > > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Get the new Windows Live Messenger! http://imagine-msn.com/messenger/launch80/default.aspx?locale=en-us&source=wlmailtagline From Rcartun <@t> harthosp.org Tue Aug 22 18:53:37 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Tue Aug 22 18:54:10 2006 Subject: [Histonet] Patient slide send-out In-Reply-To: References: <082220061927.25768.44EB5AAF000B9FE4000064A82200763692C9CEC99B9D0E08029D0E0D@comcast.net> Message-ID: <44EB60C1020000770000180C@hcnwgwds01.hh.chs> Thank you for your excellent summary of the issues relating to slide send-outs. I agree with every point you made. RWC Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Julia Dahl" 08/22/06 6:58 PM >>> I agree that there are different categories of send-outs. As a consultant pathologist who is receiving the "send-out" - an 88321 (pathology second opinion on slides prepared elsewhere) is a professional component only CPT code and does not include any TC reimbursement (including shipping of slides and/or blocks). The cost of shipping, then, should be born by the party requesting the slides. If a pathologist is requesting a consultation, then their department pays for the shipping. If it is a patient, then it's the patient... what about a clinician requesting the send out? Do you want to be telling your clinicians that they will have to pay for shipping? It's an imporant and difficult question to answer. For BOTH sides - the originating pathology institution and the receiving pathology institution - second opinion pathology consultations are UNFORTUNATELY not economically feasible within the current CPT code and reimbursement schedules. Less than 60% of insurance companies actually will pay the consultant pathologist for the consultation. This is ridiculous given that the SAME insurance company will require a physician second opinion for patients with many medical conditions and surgeries PRIOR to paying for a surgical procedure or lengthy medical treatment... AND the insurance company does pay for that second opinion. As is evidenced by the literature and through anecdotal experience - second opinion in anatomic pathology is a valuable means to ensuring pathology accuracy. Nonetheless, this valuable service is neither convenient for the home institution nor the consultant... much less economically feasible for either institution. In the time that it takes for our organization to provide a consultation on an IBD with dysplasia or collagenous colitis with co-existent IBD (a sampling of today's consultations), our pathologists can sign out at least ten 88305 gastridites or adenomas. As an organization, do we dedicate our resources to primary diagnoses (more $$/less time) or to assisting our colleagues with their challenging cases (more time/less $$)? Something that should be separated from the current discussion is the instance of a send-out for a patient who is undergoing surgery at another institution. This is a quality assurance activity. Any institution in which a patient is undergoing DEFINITIVE therapy MUST review the pathologic material upon which the definitive therapy is rendered as a matter of ensuring the proper treatment and complete medical record for that patient within the treating institution. What would be the consequence of a sclerosing papilloma on biopsy called invasive carcinoma in Institution A, with the patient sent to Institution B for lumpectomy or mastectomy with sentinel node dissection? If Institution B requests the slides PRIOR to definitive therapy, and the diagnostic error is detected - the patient does not undergo an unnecessary surgical procedure and NONE of the physicians or medical institutions are sued. If Institution B fails to re-review the slides prior to definitive therapy, the patient has an unnecessary surgery with the resultant M&M - and EVERYONE (Institution A, Institution B, Pathologist A, Pathologist B and Surgeons) gets sued and will settle out of court. With this in mind - for second opinions prior to definitive therapy, the requesting institution must pay for the "consultation" if the patient's insurance will not. The down-side for the requesting institution is that in the absence of the insurance company paying for the "second opinion" - the hospital incurs the expense, and has no mechanism for recovering this expense as the DRG is not changed. Until this service is more equitably reimbursed - all parties are in a difficult situation. Julia Dahl MD >From: barnhart717@comcast.net >To: "Zajic Kari" ,"Richard Cartun" >, >Subject: RE: [Histonet] Patient slide send-out >Date: Tue, 22 Aug 2006 19:27:43 +0000 > >When we receive a request to send blocks or slides to another facility for >any reason we send the patients insurance information to the facility. In >the letter we send we include the reason why the slides/blocks are being >sent and to charge the patient's insurance not us. If they send us a bill >we fight it. In the documentation that we keep I have different category >for why the consult was done. Pathologist: is when our pathologist send >out for a second opinion and we pay the cost. Physician, Patient or >Treatment: These we will not pay for. The other facility eats the cost or >charges the patient's insurance. We use priority mail because it usually >arrives in about 3 days, we can track the shipment and it is a minimal >cost. > >Becky Barnhart >rbarnhart@summithealth.org > >-------------- Original message -------------- >From: "Zajic Kari" > > > Richard, you are not alone! We have also seen an increase in patient and > > physician requests for "send-outs". I am not sure of charging, but I >have made a > > "send-out" policy for our department that all requests be put in writing > > (scripts for physicians)and faxed,inform the patient/office that it will >at > > least be 48 hours before it can be sent out, they must provide a >shipping > > service account (FEDEX, UPS) or physically pick up the slides. We have >also been > > being charged for these consults by the other facility so we also inform >them > > that if the insurance cannot be charged, they are responsible for the >bill. It > > seems to work but we do run into some problems now and again. If they >cannot > > provide an overnight shipping service, we will USPS mail them certified >but that > > seems to take too long for their liking (here it's around a week). > > Not having enough staff to handle the sendouts is always a problem, >hence the 48 > > hours..helps slightly. > > > > Kari :) > > > > Kari Marie Zajic HTL,MLT > > Histology Supervisor > > Palms West Hospital > > Pathology Department > > 13001 State Road Eighty > > Loxahatchee, Florida 33470 > > phone: (561)798-6036 > > telefax: (561)753-4298 > > voicemail: (561)753-4299 > > pager: (561)610-4949 > > email: Kari.Zajic@HCAHealthcare.com > > > > This email and any files transmitted with it may contain PRIVILEGED or > > CONFIDENTIAL > > information and may be read or used only by the intended recipient. If >you are > > not the intended recipient of the email or any of its attachment, please >be > > advised that you have received this email in error and that any use, > > dissemination, distribution, forwarding, printing, or copying of this >email or > > any attached files is strictly prohibited. If you have received this >email in > > error, please immediately purge it and all attachments and notify the >sender by > > reply email or contact the sender at the number listed. > > > > > > > > > > -----Original Message----- > > From: histonet-bounces@lists.utsouthwestern.edu > > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard > > Cartun > > Sent: Tuesday, August 22, 2006 2:49 PM > > To: histonet@lists.utsouthwestern.edu > > Subject: [Histonet] Patient slide send-out > > > > > > Our Anatomic Pathology Office is overwhelmed with requests from patients > > asking for their pathology slides to be sent to another medical > > institution (for second opinion, additional surgery or therapy, etc.). > > Is it legal to charge patients for this service? We don't have the > > personnel to handle these requests in a timely fashion and we can no > > longer afford to "eat" the shipping costs. Thank you. > > > > Richard > > > > Richard W. Cartun, Ph.D. > > Director, Immunopathology & Histology > > Assistant Director, Anatomic Pathology > > Hartford Hospital > > 80 Seymour Street > > Hartford, CT 06102 > > (860) 545-1596 > > (860) 545-0174 Fax > > > > > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Get the new Windows Live Messenger! http://imagine-msn.com/messenger/launch80/default.aspx?locale=en-us&source=wlmailtagline From bamur <@t> alaska.net Tue Aug 22 20:53:49 2006 From: bamur <@t> alaska.net (Barbara Murray) Date: Tue Aug 22 20:53:54 2006 Subject: [Histonet] On-line Histology Schools Message-ID: Greetings from Anchorage, Alaska Would someone please send me a list of on-line Histology Schools. Thanks in advance and have a great day! Barbara A. Murray, HT. (ASCP) The Alaska Native Medical Center Anchorage, Alaska From mike.kirby <@t> nhls.ac.za Wed Aug 23 04:44:56 2006 From: mike.kirby <@t> nhls.ac.za (Mike Kirby) Date: Wed Aug 23 04:44:51 2006 Subject: [Histonet] Who cleans your mortuary? Message-ID: <452D0F6B16AA6E4092F6D3E9A9D97A62427988@nhlsgpm002.NHLS.AC.ZA> Hi Guys. Question! Who cleans your mortuary? Swabbing the floors and tables, cleaning the refrigeration area, sinks, windows, etc. Do you have permanent, in-house trained Staff, or do you contract out to a commercial company? M.Kirby Johannesburg South Africa. ********************************************************************************** The views expressed in this email are, unless otherwise stated, those of the author and not those of the National Health Laboratory Services or its management. The information in this e-mail is confidential and is intended solely for the addressee. Access to this e-mail by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken or omitted in reliance on this, is prohibited and may be unlawful. Whilst all reasonable steps are taken to ensure the accuracy and integrity of information and data transmitted electronically and to preserve the confidentiality thereof, no liability or responsibility whatsoever is accepted if information or data is, for whatever reason, corrupted or does not reach its intended destination. *********************************************************************************** From BMolinari <@t> heart.thi.tmc.edu Wed Aug 23 05:37:35 2006 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Wed Aug 23 05:37:38 2006 Subject: [Histonet] Patient slide send-out In-Reply-To: <095327C7CDBDF64B9E9728A54799091E015CC621@ORLEV03.hca.corpad.net> Message-ID: Just curious..why this increase in patients asking for a second opinion? Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Zajic Kari Sent: Tuesday, August 22, 2006 2:05 PM To: Richard Cartun; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Patient slide send-out Richard, you are not alone! We have also seen an increase in patient and physician requests for "send-outs". I am not sure of charging, but I have made a "send-out" policy for our department that all requests be put in writing (scripts for physicians)and faxed,inform the patient/office that it will at least be 48 hours before it can be sent out, they must provide a shipping service account (FEDEX, UPS) or physically pick up the slides. We have also been being charged for these consults by the other facility so we also inform them that if the insurance cannot be charged, they are responsible for the bill. It seems to work but we do run into some problems now and again. If they cannot provide an overnight shipping service, we will USPS mail them certified but that seems to take too long for their liking (here it's around a week). Not having enough staff to handle the sendouts is always a problem, hence the 48 hours..helps slightly. Kari :) Kari Marie Zajic HTL,MLT Histology Supervisor Palms West Hospital Pathology Department 13001 State Road Eighty Loxahatchee, Florida 33470 phone: (561)798-6036 telefax: (561)753-4298 voicemail: (561)753-4299 pager: (561)610-4949 email: Kari.Zajic@HCAHealthcare.com This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard Cartun Sent: Tuesday, August 22, 2006 2:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Patient slide send-out Our Anatomic Pathology Office is overwhelmed with requests from patients asking for their pathology slides to be sent to another medical institution (for second opinion, additional surgery or therapy, etc.). Is it legal to charge patients for this service? We don't have the personnel to handle these requests in a timely fashion and we can no longer afford to "eat" the shipping costs. Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From pcprabuin <@t> yahoo.co.in Wed Aug 23 06:44:34 2006 From: pcprabuin <@t> yahoo.co.in (pcb prabu) Date: Wed Aug 23 06:44:44 2006 Subject: [Histonet] redirect to new mail id Message-ID: <20060823114437.59710.qmail@web8323.mail.in.yahoo.com> kindly enroll me as a member of histonet. please redirect the mails to pcprabuin@gmail.com thanking you dr.p.c.prabu --------------------------------- Here's a new way to find what you're looking for - Yahoo! Answers Send FREE SMS to your friend's mobile from Yahoo! Messenger Version 8. Get it NOW From rjbuesa <@t> yahoo.com Wed Aug 23 07:19:50 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Aug 23 07:19:54 2006 Subject: [Histonet] Who cleans your mortuary? In-Reply-To: <452D0F6B16AA6E4092F6D3E9A9D97A62427988@nhlsgpm002.NHLS.AC.ZA> Message-ID: <20060823121950.9198.qmail@web61212.mail.yahoo.com> Routine (weekly) cleaning was part of the diener's job description. When something more "in depth" was required the diener along with cleaning personnel from the hospital did it. Ren? J. Mike Kirby wrote: Hi Guys. Question! Who cleans your mortuary? Swabbing the floors and tables, cleaning the refrigeration area, sinks, windows, etc. Do you have permanent, in-house trained Staff, or do you contract out to a commercial company? M.Kirby Johannesburg South Africa. ********************************************************************************** The views expressed in this email are, unless otherwise stated, those of the author and not those of the National Health Laboratory Services or its management. The information in this e-mail is confidential and is intended solely for the addressee. Access to this e-mail by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken or omitted in reliance on this, is prohibited and may be unlawful. Whilst all reasonable steps are taken to ensure the accuracy and integrity of information and data transmitted electronically and to preserve the confidentiality thereof, no liability or responsibility whatsoever is accepted if information or data is, for whatever reason, corrupted or does not reach its intended destination. *********************************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your email and more, right on the new Yahoo.com From jdmd77 <@t> hotmail.com Wed Aug 23 08:01:36 2006 From: jdmd77 <@t> hotmail.com (Julia Dahl) Date: Wed Aug 23 08:01:46 2006 Subject: [Histonet] Patient slide send-out In-Reply-To: Message-ID: Again, to be transparent with my biases - I am a subspeciality pathologist, with a large proportion of my case volume in consultations and I am actively involved in the ABQAURP (a quality assurance organization) and QA initiatives in pathology. Recently within the lay media, there has been a significant influx of articles that "report" on rates of laboratory error. Last January, Katie Couric hosted a guest that spoke for 12 minutes about the use of second opinions in Medicine to support her (the guest's) book sales for a book on this topic. During the 12 minutes, she spent 6 seconds on the topic of pathology consultations. The LA Times recently published a scathing article about anatomic pathology quality assurance, the Wall Street Journal, another article about misdiagnoses and quality issues in pathology AND the GAO released a report that there needs to be more laboratory oversight, as quality is basically poor. There are pay for performance initiatives that will also drive MORE consults, not fewer, consults. QA initiatives in pathology strongly recommend that 1 - 2% of all cases be referred to subspecialty pathologists for consultation UNLESS the home institution department has representation in each of the subspecialties (i.e. GYN, dermatopathology, placenta, neuropathology, GI/Liver, endocrine, cytopath, hemepath, etc.). If your institution has a volume of 15,000 cases/year - this translates to 150 - 300 send outs a year or 1 - 2 each DAY. The same QA literature notes that only 30% of instititions actually meet this QA benchmark. With this in mind - planning for the volume being sent out is imperative - to ensure the highest quality of care for each patient and to ensure the financial feasibility within the department. Pathologists, histologists, hospital QA committees and others also MUST take a more active role in defending the "billability" of consultations to insurance companies. Did the media ever tell the patients what the second opinion would cost? No. They reported that second opinions in pathology are necessary.... and patients ASSUME that their insurance will pay for it. Well, two of the major insurance conglomerates that represent millions of patient's lives don't pay for consultations in pathology. These conglomerates also select the laboratory for the patient and the clinicians with their exclusive laboratory agreements in which (unless performed in a hospital) the patient's biopsy material MUST be referred to their "preferred" laboratory. Interesting. I believe that the push for more send-outs - and this is just an observation, not supported by research - that more clinicians are becoming aware of subspecialty pathologists. There are large conglomerate laboratories and other laboratories that are aggressively marketing subspecialty pathology services within a general pathology environment - or dedicated solely to ONE subspecialty to clinicians. Clinicians will then pass along this marketing to their patient to advance their own quality in the patient's eyes --- "The biopsy is going to be sent to a lab that specializes in this type of disease and only looks at 'x' cases." For many of these organizations - much, much more money within the laboratory is spent on marketing and management than on paying pathologists and techs. And this trend will continue, too. There are a lot of published articles about why 2nd opinion in pathology is a very, very good thing. Rates of error when subspecialty pathologists re-review general pathologist's work can be fairly significant - in GI/Liver 7.8% of all cases contain a medically significant error; 12.5% of Barrett's cases, 45-54% of IBD and up to 62% of medical diseases of the liver. Does that translate to general pathologists being "bad" at GI? Absolutely not. General pathologists must be facile in ALL of the organ systems, while subspecialists can focus on the nuances of just ONE. So much for sending a brief overview.... but I hope this gives some insight, Julia Dahl, M.D. Mosaic Gastrointestinal Research Consortium jdahlmd@mosaicgi.com >Just curious..why this increase in patients asking for a second opinion? > >Betsy Molinari HT (ASCP) >Texas Heart Institute >Cardiovascular Pathology >6770 Bertner Ave. >Houston,TX 77030 >832-355-6524 >832-355-6812 (fax) > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Zajic >Kari >Sent: Tuesday, August 22, 2006 2:05 PM >To: Richard Cartun; histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] Patient slide send-out > >Richard, you are not alone! We have also seen an increase in patient and >physician requests for "send-outs". I am not sure of charging, but I >have made a "send-out" policy for our department that all requests be >put in writing (scripts for physicians)and faxed,inform the >patient/office that it will at least be 48 hours before it can be sent >out, they must provide a shipping service account (FEDEX, UPS) or >physically pick up the slides. We have also been being charged for these >consults by the other facility so we also inform them that if the >insurance cannot be charged, they are responsible for the bill. It seems >to work but we do run into some problems now and again. If they cannot >provide an overnight shipping service, we will USPS mail them certified >but that seems to take too long for their liking (here it's around a >week). >Not having enough staff to handle the sendouts is always a problem, >hence the 48 hours..helps slightly. > >Kari :) > >Kari Marie Zajic HTL,MLT >Histology Supervisor >Palms West Hospital >Pathology Department >13001 State Road Eighty >Loxahatchee, Florida 33470 >phone: (561)798-6036 >telefax: (561)753-4298 >voicemail: (561)753-4299 >pager: (561)610-4949 >email: Kari.Zajic@HCAHealthcare.com > >This email and any files transmitted with it may contain PRIVILEGED or >CONFIDENTIAL >information and may be read or used only by the intended recipient. If >you are not the intended recipient of the email or any of its >attachment, please be advised that you have received this email in error >and that any use, dissemination, distribution, forwarding, printing, or >copying of this email or any attached files is strictly prohibited. If >you have received this email in error, please immediately purge it and >all attachments and notify the sender by reply email or contact the >sender at the number listed. > > > > >-----Original Message----- >From: histonet-bounces@lists.utsouthwestern.edu >[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard >Cartun >Sent: Tuesday, August 22, 2006 2:49 PM >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] Patient slide send-out > > >Our Anatomic Pathology Office is overwhelmed with requests from patients >asking for their pathology slides to be sent to another medical >institution (for second opinion, additional surgery or therapy, etc.). >Is it legal to charge patients for this service? We don't have the >personnel to handle these requests in a timely fashion and we can no >longer afford to "eat" the shipping costs. Thank you. > >Richard > >Richard W. Cartun, Ph.D. >Director, Immunopathology & Histology >Assistant Director, Anatomic Pathology >Hartford Hospital >80 Seymour Street >Hartford, CT 06102 >(860) 545-1596 >(860) 545-0174 Fax > > > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Windows Live Spaces is here! It’s easy to create your own personal Web site. http://spaces.live.com/signup.aspx From TMcNemar <@t> lmhealth.org Wed Aug 23 08:29:23 2006 From: TMcNemar <@t> lmhealth.org (Tom McNemar) Date: Wed Aug 23 08:30:25 2006 Subject: [Histonet] On-line Histology Schools Message-ID: <51D5D78FBEDAEA4FBCCD9A9D44211DC528F3ED@lmhsmail.lmhealth.org> You can check out http://www.cscc.edu/Histology/index.htm It's run by Columbus State (Ohio). Tom McNemar, HT(ASCP) Histology Co-ordinator Licking Memorial Health Systems (740) 348-4163 (740) 348-4166 tmcnemar@lmhealth.org www.LMHealth.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Barbara Murray Sent: Tuesday, August 22, 2006 9:54 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] On-line Histology Schools Greetings from Anchorage, Alaska Would someone please send me a list of on-line Histology Schools. Thanks in advance and have a great day! Barbara A. Murray, HT. (ASCP) The Alaska Native Medical Center Anchorage, Alaska _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From nellisk <@t> mail.nih.gov Wed Aug 23 08:44:38 2006 From: nellisk <@t> mail.nih.gov (Nellis, Kevin Lee (NIH/NCI) [E]) Date: Wed Aug 23 08:44:48 2006 Subject: [Histonet] Histopathology Technician / Archivist position available Message-ID: Dear All: We have a job opening for a Histopathology Technician (NCI), GS-8 ($40,612.00 - 52,794.00). This position has a full promotion potential up to a GS-10 ($49,397-$64,213). The incumbent will process tissues, release human biological materials for research purposes, and archive patient specimens. The incumbent will operate histology equipment, perform quality control and maintenance, prepare reagents, change solutions, and enter data in various computer applications. The incumbent provides service to a wide variety of professions and serves as a central source of information for the Laboratory of Pathology at the National Cancer Institute (Bethesda, MD) regarding medical legal requests and requests for human biological materials. Maintains a human pathology archive and communicates procedures and resources available to research investigators. Go to www.usajobs.gov & search for NCI-06-142102-DE in the Keyword search field. Be sure to submit resume and provide the KSA information located in the Qualification & Evaluation section of the posting. Best regards, Kevin Kevin L. Nellis, M.S., M.T. (A.S.C.P.) Clinical Laboratory Manager Laboratory of Pathology Center for Cancer Research National Cancer Institute National Institutes of Health U.S. Department of Health and Human Services 10 Center Drive, Room 2A33, MSC 1500 Bethesda, MD 20892 OFFICE: (301) 594-9532 FAX: (301) 402-0043 http://home.ncifcrf.gov/ccr/lop/Clinical/default.asp From TJasper <@t> smdc.org Wed Aug 23 08:54:12 2006 From: TJasper <@t> smdc.org (Jasper, Thomas G.) Date: Wed Aug 23 08:54:33 2006 Subject: [Histonet] Who cleans your mortuary? In-Reply-To: <452D0F6B16AA6E4092F6D3E9A9D97A62427988@nhlsgpm002.NHLS.AC.ZA> Message-ID: <1A9F2A6C5762524799A816F1F09744CF1E0B72@SCREECH.ntcampus.smdc.org> Hi Mike, We have our Environmental Services Department (Housekeepers) handle this job. If this is a question for your institution I guess you'd have to calculate the cost internal vs. external? It's never been a question here. Tom J. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Mike Kirby Sent: Wednesday, August 23, 2006 4:45 AM To: Histonet (E-mail) Subject: [Histonet] Who cleans your mortuary? Hi Guys. Question! Who cleans your mortuary? Swabbing the floors and tables, cleaning the refrigeration area, sinks, windows, etc. Do you have permanent, in-house trained Staff, or do you contract out to a commercial company? M.Kirby Johannesburg South Africa. ********************************************************************************** The views expressed in this email are, unless otherwise stated, those of the author and not those of the National Health Laboratory Services or its management. The information in this e-mail is confidential and is intended solely for the addressee. Access to this e-mail by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken or omitted in reliance on this, is prohibited and may be unlawful. Whilst all reasonable steps are taken to ensure the accuracy and integrity of information and data transmitted electronically and to preserve the confidentiality thereof, no liability or responsibility whatsoever is accepted if information or data is, for whatever reason, corrupted or does not reach its intended destination. *********************************************************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From hakim <@t> bgu.ac.il Wed Aug 23 09:26:43 2006 From: hakim <@t> bgu.ac.il (Vicki Hakim) Date: Wed Aug 23 09:26:56 2006 Subject: [Histonet] p-0 fixation and embedding Message-ID: hi all we are interested in a protocol for fixation and embedding for P-0 mouse brains. if u have a recommendation on 1) the preferable fixative (PFA or formalin) and the specific times for fixation with each substance 2) time in the melted paraffin 3) xylene or toluene thanks in advance vicki ? From bill501 <@t> mindspring.com Wed Aug 23 10:12:02 2006 From: bill501 <@t> mindspring.com (Bill) Date: Wed Aug 23 10:10:30 2006 Subject: [Histonet] Patient slide send-out In-Reply-To: References: Message-ID: At 8:01 AM -0500 8/23/06, Julia Dahl wrote: >So much for sending a brief overview.... but I hope this gives some insight, Julia, This is an excellent overview of a complex, changing situation which may be the straw that puts me out of business if it becomes legislated. I am a solo pathologist with 30+ years of experience who serves several small rural hospitals (too small to have an on site pathologist). I do about 3000 cases a year primarily GI, breast, GYN and skin. My background is in Neuropathology and gen Surg Path. I send out between 50-100 cases a year, both consults I want and slides reviewed by large institutions where primary therapy is being done and cases requiring immunohistochemistry since we are not large enough to do it ourselves. All of this I use as peer review (quality assurance if you will). We also use a number of devices to minimize mailing costs. Being rural, it is not usually the clinicians or patients that initiate consults (2nd opinions), but me, as I know my limitations and in many circumstances working solo causes a gut feelings that someone else needs to look at a case. The statistics you mention sound troublesome, but one MUST remember that such numbers only apply to large groups of pathologists and not to individuals. Unfortunately they will be used to control the behavior of individuals which will make the numbers look better while doing little to ensure accuracy in individual cases. Nor do they take into account case mixes. Sending out 2-3% of hernia sacs or SK/BINs would not be of much use. Bill From wjkost <@t> anthc.org Wed Aug 23 10:43:15 2006 From: wjkost <@t> anthc.org (Kost, William J) Date: Wed Aug 23 10:43:20 2006 Subject: [Histonet] Histology Jobs in Alaska! Message-ID: Good Afternoon All, I wanted to let you know about 2 excellent opportunities in beautiful Anchorage, Alaska. The Alaska Native Medical Center is seeking a full time Histology Technician and a full time Lead Histology Technician for our Laboratory, which is accredited with distinction by the College of American Pathologists. The Alaska Native Medical Center (ANMC) is a 150-bed Alaska Native owned and managed hospital and a Native place for healing and learning. Here, advanced technology meets human caring for members of the 229 tribes of Alaska. ANMC combines big-city expertise, facilities and equipment with the attention to each individual typical of a rural Alaska village. We provide a patient centered, relationship-based system with same-day access to care, and greater emphasis on wellness and health promotion. ANMC is a Native gathering place where long-time friends can celebrate life events, learn ways to maintain wellness or simply visit one another. ANMC is Alaska's only Level II Trauma Center, a title that certifies its ability to provide quality care to people with traumatic injuries 24 hours a day, 365 days a year. ANMC is the proud recipient of the Nursing Magnet Status award. This is the highest honor in Professional nursing. ANMC is the 71st hospital and the first tribally owned and operated facility in the nation to be awarded this prestigious honor. Please feel free to visit our website @ www.anthc.org or call me at any time, if you would like more information or have any questions. William J. Kost - (907) 729-1331. Thank you and I look forward to hearing from you! "ANTHC . . . A GREAT PLACE TO WORK AND GROW" William J. Kost, PHR Professional Recruiter ANMC/ANTHC Office of Human Resources (907) 729-1331 (907) 729-3638 Fax wjkost@anthc.org This E-mail message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply E-mail, and destroy all copies of the original message. From JosefaNava <@t> texashealth.org Wed Aug 23 12:50:30 2006 From: JosefaNava <@t> texashealth.org (Nava, Josefa) Date: Wed Aug 23 12:54:21 2006 Subject: [Histonet] Looking for a new vendor for Amyloid P Antibody Message-ID: <2C515C1049EAF5459EFD8C9B929078A401DA13A6@phdex03.txhealth.org> Hello, Can someone tell me where I can order Amyloid P antibody. Dako and Novocastra have discontinued it. I will appreciate any information you can give me. I will be running the Amyloid P on a Ventana machine and VBS Bond Max. Thank you. Josie The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From lpstirpe <@t> yahoo.com Wed Aug 23 12:54:51 2006 From: lpstirpe <@t> yahoo.com (Linda Stirpe) Date: Wed Aug 23 12:55:21 2006 Subject: [Histonet] Disposal of surgical specimens Message-ID: <20060823175451.94510.qmail@web54614.mail.yahoo.com> I know this subject has been previously discussed but now it has become an issue at our facility. We are a small hospital processing about 5,000 surgicals/year. Currently, we dispose of the specimens with fixative by placing them in biohazard containers and then hauled off site. The safety committee now has concerns that the specimen is a biohazard and the formain is a hazardous waste and should be separated for disposal purposes. We are interested in how other hospitals handle this. Any suggestions would be greatly appreciated. Thank you! Linda Stirpe, HT(ASCP) Corning Hospital Corning, NY __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From AGrobe2555 <@t> aol.com Wed Aug 23 13:49:08 2006 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Wed Aug 23 13:49:16 2006 Subject: [Histonet] eNOS evaluation Message-ID: <57c.3e0afac.321dfd24@aol.com> Tahseen, I have worked with eNOS in the past. Can you give me a bit more detail of what you are looking for? Cellular localization, levels, NO production, phosphorylation state, etc....? Albert From rjbuesa <@t> yahoo.com Wed Aug 23 13:54:26 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Aug 23 13:54:39 2006 Subject: [Histonet] Disposal of surgical specimens In-Reply-To: <20060823175451.94510.qmail@web54614.mail.yahoo.com> Message-ID: <20060823185426.21282.qmail@web61218.mail.yahoo.com> Linda: We used to separate formalin from the tissue. Formalin was placed in large drums to be hauled away by a company we contracted. The tissues were incinerated at the hospital (something that probably you will not be able to do). Otherwise the tissue should be handled as any other biohazard sample within the lab. Hope this will help you! Ren? J. Linda Stirpe wrote: I know this subject has been previously discussed but now it has become an issue at our facility. We are a small hospital processing about 5,000 surgicals/year. Currently, we dispose of the specimens with fixative by placing them in biohazard containers and then hauled off site. The safety committee now has concerns that the specimen is a biohazard and the formain is a hazardous waste and should be separated for disposal purposes. We are interested in how other hospitals handle this. Any suggestions would be greatly appreciated. Thank you! Linda Stirpe, HT(ASCP) Corning Hospital Corning, NY __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Want to be your own boss? Learn how on Yahoo! Small Business. From victoria.spoon <@t> bassett.org Wed Aug 23 14:22:06 2006 From: victoria.spoon <@t> bassett.org (Spoon, Victoria) Date: Wed Aug 23 14:53:08 2006 Subject: [Histonet] diener pay Message-ID: <415700FC732DE14491A3E39367834F7715AD6E@ex3.bassett.org> What is the going rate of pay for dieners? Thanks in advance. NOTICE OF CONFIDENTIALITY This electronic message, including attachments, is for the sole use of the named recipient and may contain confidential or privileged information protected by New York State, and Federal regulations. Any unauthorized review, use, disclosure, copying or distribution is strictly prohibited. If you are not the intended recipient or have received this communication in error please contact the sender or email.security@bassett.org and destroy all copies of the original message. Thank you. From NMargaryan <@t> childrensmemorial.org Wed Aug 23 16:38:08 2006 From: NMargaryan <@t> childrensmemorial.org (Margaryan, Naira) Date: Wed Aug 23 16:34:38 2006 Subject: [Histonet] RE: Histonet Digest, Vol 33, Issue 28 Message-ID: <63B8B599DE283148B92E83C78B32C15D02E0DFEE@cmhexbe2.childrensmemorial.org> Me too, please :-) And, please, not only histology, but cancer pathology as well!!! Thanks, Naira Naira V. Margaryan, D.V.M., Ph.D. Research Associate III Children's Memorial Research Center 2300 Children's Plaza, Box 222 Chicago, IL 60614-3394 Tel: 773-755-6570/ext-8 Fax: 773-755-6594 nmargaryan@childrensmemorial.org For Express Mail: CMRC, Room C.473 2430 N. Halsted Street Chicago, IL 60614-4314 ------------------------------ Message: 10 Date: Tue, 22 Aug 2006 17:53:49 -0800 From: "Barbara Murray" Subject: [Histonet] On-line Histology Schools To: Message-ID: Content-Type: text/plain; charset="iso-8859-1" Greetings from Anchorage, Alaska Would someone please send me a list of on-line Histology Schools. Thanks in advance and have a great day! Barbara A. Murray, HT. (ASCP) The Alaska Native Medical Center Anchorage, Alaska From tncperfect2 <@t> comcast.net Wed Aug 23 17:09:24 2006 From: tncperfect2 <@t> comcast.net (tncperfect2@comcast.net) Date: Wed Aug 23 17:09:33 2006 Subject: [Histonet] Cost of consumables Message-ID: <082320062209.25134.44ECD214000AC3560000622E2213575333CD9B0C0A009D0A9F0C029B@comcast.net> Hi Histonetters. I need to know how much managers are spending on consumables that deal with 1000 or more specimens a month...give or take a few dollars? I just need a ball park figure for now. We do just the H&E routine stain. If anyone can help me it would be very much appreciated. Thanks, Carolyn From mobine <@t> MIT.EDU Wed Aug 23 17:16:46 2006 From: mobine <@t> MIT.EDU (Hector Mobine) Date: Wed Aug 23 17:16:54 2006 Subject: [Histonet] Rat Histology Message-ID: <200608232216.k7NMGkix025572@outgoing.mit.edu> I am in the process of designing histological experiments of rat and mice hearts and would be interested in a good description of rat heart excision and anatomy. I have yet been unable to find one in medical textbooks, reviews or protocols. Does anyone know of any good references, pictures, books or protocols? Any help would be appreciated. Thanks, Hector Graduate Student Edelman Lab Biological Engineering Division Massachusetts Institute of Technology From AGrobe2555 <@t> aol.com Wed Aug 23 17:27:22 2006 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Wed Aug 23 17:27:31 2006 Subject: [Histonet] Hydrogen peroxide sterilization Message-ID: <414.8c46d5a.321e304a@aol.com> Does anyone know of a company which will do H2O2 gas-phase sterilization on a contract basis? Please feel free to contact me directly off-list. Thanks, Albert From bamur <@t> alaska.net Wed Aug 23 18:29:15 2006 From: bamur <@t> alaska.net (Barbara Murray) Date: Wed Aug 23 18:29:20 2006 Subject: [Histonet] Histo Position Message-ID: Greetings from Anchorage, Alaska. We currently have two openings in Histology @ The Alaska Native Medical Center. Contact David Morrison, Laboratory Manager @ 907-729-1228 for details and $$$$$$! Have a great day! Barbara A. Murray, HT. (ASCP) The Alaska Native Medical Center Anchorage, Alaska 99508 From Godsgalnow <@t> aol.com Wed Aug 23 19:56:22 2006 From: Godsgalnow <@t> aol.com (Godsgalnow@aol.com) Date: Wed Aug 23 19:56:42 2006 Subject: [Histonet] diener pay Message-ID: <516.60e833f.321e5336@aol.com> I have always paid per case.....$250 - $300 per case. It doesn't matter if it is a chest only or a full body, the pay is the same. Roxanne From Godsgalnow <@t> aol.com Wed Aug 23 20:01:19 2006 From: Godsgalnow <@t> aol.com (Godsgalnow@aol.com) Date: Wed Aug 23 20:01:30 2006 Subject: [Histonet] Cost of consumables Message-ID: <37d.9d06a47.321e545f@aol.com> This is all so subjective. It depends on where you are located, how much you pay for shipping, if at all. If you have a GPO or multiple vendors that you use. Progressive or regressive staining? Gills, Harris, Clarifier or no? Bluing or no? Plus slides, regular slides? Do you cut extras? Do you have to pay for DI water? Do you have a specific brand of products you use? Roxanne Soto Lab Manager Physicians RightPath Tampa, Florida 813-549-1050 From JMacDonald <@t> mtsac.edu Wed Aug 23 22:32:12 2006 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Wed Aug 23 22:32:34 2006 Subject: [Histonet] Looking for a new vendor for Amyloid P Antibody Message-ID: I know that BioCare has it. You can also check Cell Marque. -----hist To: Sen Date: 08/23/2006 10:50AM Subject: [Histonet] Looking for a new vendor for Amyloid P Antibody Hello, Can someone tell me where I can order Amyloid and Novocastra have discontinued &nb information you can give me. I will be runn and VBS Bond Max. &nb Josie The information contained in this mes intended only for the use of the individual or addressed, and may contain information that is PRIVIL CONFIDENTIAL, and exempt from disclosure under applicable law. you are not the intended recipient, you are prohibited from copying, dis immedi your system. _______________________ 5F _______________________ Hi Histonet@lists.utsouthwestern.edu [1]htt References 1. file://localhost/tmp/3D"http From zumbor <@t> email.cs.nsw.gov.au Thu Aug 24 00:39:39 2006 From: zumbor <@t> email.cs.nsw.gov.au (Rosalba) Date: Thu Aug 24 00:42:50 2006 Subject: [Histonet] Who cleans your mortuary? Message-ID: <01C6C793.83411BC0.zumbor@email.cs.nsw.gov.au> Hi All, The mortuary technicians who assist with the post-mortems clean the mortuary after they have finished their cases. Each cleans their own work area and there is a roster for cleaning the other general areas such as the fridge etc. Rosalba Zumbo Supervisor Histology Dept Department of Forensic Medicine 42-50 Parramatta Rd Glebe NSW 2037 Australia PH: 61 2 85847842 FAX: 61 2 95664573 -----Original Message----- From: Mike Kirby [SMTP:mike.kirby@nhls.ac.za] Sent: Wednesday, 23 August 2006 7:45 PM To: Histonet (E-mail) Subject: [Histonet] Who cleans your mortuary? Hi Guys. Question! Who cleans your mortuary? Swabbing the floors and tables, cleaning the refrigeration area, sinks, windows, etc. Do you have permanent, in-house trained Staff, or do you contract out to a commercial company? M.Kirby Johannesburg South Africa. ************************************************************************ ********** The views expressed in this email are, unless otherwise stated, those of the author and not those of the National Health Laboratory Services or its management. The information in this e-mail is confidential and is intended solely for the addressee. Access to this e-mail by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken or omitted in reliance on this, is prohibited and may be unlawful. Whilst all reasonable steps are taken to ensure the accuracy and integrity of information and data transmitted electronically and to preserve the confidentiality thereof, no liability or responsibility whatsoever is accepted if information or data is, for whatever reason, corrupted or does not reach its intended destination. ************************************************************************ *********** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _____________________________________________________________________ This email has been scanned for the Sydney South West Area Health Service by the MessageLabs Email Security System. SSWAHS regularly monitors emails and attachments to ensure compliance with the NSW Government's Electronic Messaging Policy. "This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please destroy it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of the Sydney South West Area Health Service." From Lynda.King <@t> vision-bio.com Thu Aug 24 01:15:45 2006 From: Lynda.King <@t> vision-bio.com (Lynda King) Date: Thu Aug 24 01:16:04 2006 Subject: [Histonet] unsubscribe Message-ID: <5E06BFED29594F4C9C5EBE230DE320C6114B5DBC@ewok.vsl.com.au> Lynda King Customer and Sales Administrator Vision BioSystems Ltd 495 Blackburn Road Mt Waverley VIC 3149 Australia telephone: +61 3 9211 7400 facsimile: +61 3 9211 7401 www.vision-bio.com IMPORTANT - This email and any attachments may be confidential. Any retransmissions, dissemination or other use of these materials by persons or entities other than the intended recipient is prohibited. If received in error, please contact us and delete all copies. Before opening or using attachments, check them for viruses and defects. Our liability is limited to resupplying any affected attachments. [Any representations or opinions expressed in this email are those of the individual sender, and not necessarily those of Vision Systems Limited]. From hbeard27 <@t> hotmail.com Thu Aug 24 03:18:32 2006 From: hbeard27 <@t> hotmail.com (Helen Beard) Date: Thu Aug 24 03:18:40 2006 Subject: [Histonet] F4/80 In-Reply-To: <200608232216.k7NMGkix025572@outgoing.mit.edu> Message-ID: I would be interested in hearing from anyone who has used Sertec F4/80 to do IHC for microglia in pfa fixed mouse brains. I have lovely staining in my kupfer cells (control) but only staining of what appears to be neurones in the brain. A protocol would be greatly appreciated. Thanks in advance Helen From hakim <@t> bgu.ac.il Thu Aug 24 04:52:28 2006 From: hakim <@t> bgu.ac.il (Vicki Hakim) Date: Thu Aug 24 04:52:46 2006 Subject: [Histonet] p-0 fixation and embedding-correction In-Reply-To: <6.0.0.22.1.20060823110248.01b84e38@gemini.msu.montana.edu> References: <6.0.0.22.1.20060823110248.01b84e38@gemini.msu.montana.edu> Message-ID: sorry for the abbreviation, p-0 means p- zero (immediately after birth) thanks again vicki ----- Original Message ----- From: Gayle Callis Date: Wednesday, August 23, 2006 19:03 Subject: Re: [Histonet] p-0 fixation and embedding To: Vicki Hakim > Define what P-O mouse brains means, please. > > At 08:26 AM 8/23/2006, you wrote: > >hi all > >we are interested in a protocol for fixation and embedding for > P-0 mouse > >brains. if u have a recommendation on 1) the preferable > fixative (PFA or > >formalin) and the specific times for fixation with each > substance 2) time > >in the melted paraffin 3) xylene or toluene > >thanks in advance > >vicki > > > > > > ??? > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 > 406 994-4303 (FAX) > > > ? From BMolinari <@t> heart.thi.tmc.edu Thu Aug 24 06:09:26 2006 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Thu Aug 24 06:09:31 2006 Subject: [Histonet] Disposal of surgical specimens In-Reply-To: <20060823185426.21282.qmail@web61218.mail.yahoo.com> Message-ID: Linda, We do the same here. Our tissue is stored in "seal a meal" Heavy Duty Kapak bags until the investigator is ready to let it go. Then we cut a small corner off the bag, dump the formalin in a waste container, reseal the bag and put it into a biohazard bag for the hospital to dispose of. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, August 23, 2006 1:54 PM To: Linda Stirpe; HistoNet Server Subject: Re: [Histonet] Disposal of surgical specimens Linda: We used to separate formalin from the tissue. Formalin was placed in large drums to be hauled away by a company we contracted. The tissues were incinerated at the hospital (something that probably you will not be able to do). Otherwise the tissue should be handled as any other biohazard sample within the lab. Hope this will help you! Ren? J. Linda Stirpe wrote: I know this subject has been previously discussed but now it has become an issue at our facility. We are a small hospital processing about 5,000 surgicals/year. Currently, we dispose of the specimens with fixative by placing them in biohazard containers and then hauled off site. The safety committee now has concerns that the specimen is a biohazard and the formain is a hazardous waste and should be separated for disposal purposes. We are interested in how other hospitals handle this. Any suggestions would be greatly appreciated. Thank you! Linda Stirpe, HT(ASCP) Corning Hospital Corning, NY __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Want to be your own boss? Learn how on Yahoo! Small Business. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Tanni.Ahmed <@t> intervet.com Thu Aug 24 06:15:55 2006 From: Tanni.Ahmed <@t> intervet.com (Ahmed, T (Tanni)) Date: Thu Aug 24 06:21:03 2006 Subject: [Histonet] IHC - sections falling off! Message-ID: <11DBF8AFF1DC0F4A9B51A23F8E5BDA33799067@mksn83.d50.intra> Dear All, I am trying to save sections of canine mammary tissue from falling off in antigen retrieval solution in a microwave technique (have also tried half power microwaving). I am using normal superfrost slides and leaving them to incubate overnight. The sections are fine after dewaxing - therefore I believe the type of slide used whether it be superfrost or positive charged polysaline slides should not matter? My other option is maybe to use non heat-treatment methods of enzyme digestion such as trypsin. Is there anyone who has any ideas to prevent sections falling off during the microwave retrieval step? Any suggestions to improve are welcome. Thanks in advance, Tanni -------------------------------------- This message, including attachments, is confidential and may be privileged. If you are not an intended recipient, please notify the sender then delete and destroy the original message and all copies. You should not copy, forward and/or disclose this message, in whole or in part, without permission of the sender. -------------------------------------- From wellborj <@t> mercyhealth.com Thu Aug 24 07:18:03 2006 From: wellborj <@t> mercyhealth.com (Janci Wellborn) Date: Thu Aug 24 07:18:25 2006 Subject: [Histonet] Disposal of surgical specimens Message-ID: Linda, We separate the formalin from the tissue. The formalin is then neutralized and solidified with product called ISOSORB. The tissue and solid formalin waste is then doubled bagged and disposed of as any other biohazard sample. Our limbs are incinerated by a local mortuary. Janci Wellborn, HTL, BS, BSeD Dunes Medical Lab Dakota Dunes, SD >>> "Rene J Buesa" 8/23/2006 1:54 PM >>> Linda: We used to separate formalin from the tissue. Formalin was placed in large drums to be hauled away by a company we contracted. The tissues were incinerated at the hospital (something that probably you will not be able to do). Otherwise the tissue should be handled as any other biohazard sample within the lab. Hope this will help you! Ren? J. Linda Stirpe wrote: I know this subject has been previously discussed but now it has become an issue at our facility. We are a small hospital processing about 5,000 surgicals/year. Currently, we dispose of the specimens with fixative by placing them in biohazard containers and then hauled off site. The safety committee now has concerns that the specimen is a biohazard and the formain is a hazardous waste and should be separated for disposal purposes. We are interested in how other hospitals handle this. Any suggestions would be greatly appreciated. Thank you! Linda Stirpe, HT(ASCP) Corning Hospital Corning, NY __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Want to be your own boss? Learn how on Yahoo! Small Business. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Thu Aug 24 08:14:10 2006 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Thu Aug 24 08:14:15 2006 Subject: [Histonet] IHC - sections falling off! Message-ID: Tanni, Don't underestimate the power of a slide with extra adhesive properties. Try out Goldfrost Plus Slides or the Silanized slides from Dako. Also, try extending the time that you dry your slides before you start your procedure. Keep in mind that enzyme digestion can make tissue sections fall off as well. Good Luck, Glen Dawson BS, HT & QIHC (ASCP) IHC Manager Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Ahmed, T (Tanni) Sent: Thursday, August 24, 2006 5:16 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC - sections falling off! Dear All, I am trying to save sections of canine mammary tissue from falling off in antigen retrieval solution in a microwave technique (have also tried half power microwaving). I am using normal superfrost slides and leaving them to incubate overnight. The sections are fine after dewaxing - therefore I believe the type of slide used whether it be superfrost or positive charged polysaline slides should not matter? My other option is maybe to use non heat-treatment methods of enzyme digestion such as trypsin. Is there anyone who has any ideas to prevent sections falling off during the microwave retrieval step? Any suggestions to improve are welcome. Thanks in advance, Tanni -------------------------------------- This message, including attachments, is confidential and may be privileged. If you are not an intended recipient, please notify the sender then delete and destroy the original message and all copies. You should not copy, forward and/or disclose this message, in whole or in part, without permission of the sender. -------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From coleman_manufacturing <@t> yahoo.com Thu Aug 24 08:18:31 2006 From: coleman_manufacturing <@t> yahoo.com (Coleman Manufacturing) Date: Thu Aug 24 08:18:37 2006 Subject: [Histonet] Refurbished instruments with CMD Message-ID: <20060824131831.20959.qmail@web37614.mail.mud.yahoo.com> Tto provide every laboratory and hospital with the opportunity to enjoy the many benefits of our products, CMD is delighted to offer factory-refurbished instruments at significantly discounted prices. All of these instruments are backed by a full one-year parts and service warranty. Offer subject to availability. Equipment of the following types: Cover Slippers Cryostats Microtomes Embedding centers Tissue Processors Stainers Laboratory Microwave Systems You can request more information on the available equipment by contacting our office at: Coleman Manufacturing & Design 2852 Old Airport Road Winnsboro, SC 29180 803.633.2124 office 803.635.9401 fax Coleman_Manufacturing@yahoo.com From Rcartun <@t> harthosp.org Thu Aug 24 08:25:00 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Thu Aug 24 08:25:30 2006 Subject: [Histonet] Patient slide send-out In-Reply-To: References: <095327C7CDBDF64B9E9728A54799091E015CC621@ORLEV03.hca.corpad.net> Message-ID: <44ED706C020000770000187A@gwmail.harthosp.org> Today, patients are more educated regarding health care issues and no longer settle for what their physician tells them. Here in the East, many patients want their diagnoses confirmed by major cancer centers in New York and Boston. Also, they may be looking for alternative treatments. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Molinari, Betsy" 08/23/06 6:37 AM >>> Just curious..why this increase in patients asking for a second opinion? Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Zajic Kari Sent: Tuesday, August 22, 2006 2:05 PM To: Richard Cartun; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Patient slide send-out Richard, you are not alone! We have also seen an increase in patient and physician requests for "send-outs". I am not sure of charging, but I have made a "send-out" policy for our department that all requests be put in writing (scripts for physicians)and faxed,inform the patient/office that it will at least be 48 hours before it can be sent out, they must provide a shipping service account (FEDEX, UPS) or physically pick up the slides. We have also been being charged for these consults by the other facility so we also inform them that if the insurance cannot be charged, they are responsible for the bill. It seems to work but we do run into some problems now and again. If they cannot provide an overnight shipping service, we will USPS mail them certified but that seems to take too long for their liking (here it's around a week). Not having enough staff to handle the sendouts is always a problem, hence the 48 hours..helps slightly. Kari :) Kari Marie Zajic HTL,MLT Histology Supervisor Palms West Hospital Pathology Department 13001 State Road Eighty Loxahatchee, Florida 33470 phone: (561)798-6036 telefax: (561)753-4298 voicemail: (561)753-4299 pager: (561)610-4949 email: Kari.Zajic@HCAHealthcare.com This email and any files transmitted with it may contain PRIVILEGED or CONFIDENTIAL information and may be read or used only by the intended recipient. If you are not the intended recipient of the email or any of its attachment, please be advised that you have received this email in error and that any use, dissemination, distribution, forwarding, printing, or copying of this email or any attached files is strictly prohibited. If you have received this email in error, please immediately purge it and all attachments and notify the sender by reply email or contact the sender at the number listed. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard Cartun Sent: Tuesday, August 22, 2006 2:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Patient slide send-out Our Anatomic Pathology Office is overwhelmed with requests from patients asking for their pathology slides to be sent to another medical institution (for second opinion, additional surgery or therapy, etc.). Is it legal to charge patients for this service? We don't have the personnel to handle these requests in a timely fashion and we can no longer afford to "eat" the shipping costs. Thank you. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Thu Aug 24 09:02:11 2006 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Aug 24 09:02:47 2006 Subject: [Histonet] Disposal of surgical specimens In-Reply-To: <20060823185426.21282.qmail@web61218.mail.yahoo.com> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6D53@EMAIL.archildrens.org> We pour the formalin off of the specimens. The specimens are discarded and hauled off by the waste company. The spent formalin is also taken by another waste company for disposal. It's a pain. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Wednesday, August 23, 2006 1:54 PM To: Linda Stirpe; HistoNet Server Subject: Re: [Histonet] Disposal of surgical specimens Linda: We used to separate formalin from the tissue. Formalin was placed in large drums to be hauled away by a company we contracted. The tissues were incinerated at the hospital (something that probably you will not be able to do). Otherwise the tissue should be handled as any other biohazard sample within the lab. Hope this will help you! Ren? J. Linda Stirpe wrote: I know this subject has been previously discussed but now it has become an issue at our facility. We are a small hospital processing about 5,000 surgicals/year. Currently, we dispose of the specimens with fixative by placing them in biohazard containers and then hauled off site. The safety committee now has concerns that the specimen is a biohazard and the formain is a hazardous waste and should be separated for disposal purposes. We are interested in how other hospitals handle this. Any suggestions would be greatly appreciated. Thank you! Linda Stirpe, HT(ASCP) Corning Hospital Corning, NY __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Want to be your own boss? Learn how on Yahoo! Small Business. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From gcallis <@t> montana.edu Thu Aug 24 10:09:13 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Aug 24 10:09:20 2006 Subject: [Histonet] F4/80 In-Reply-To: References: <200608232216.k7NMGkix025572@outgoing.mit.edu> Message-ID: <6.0.0.22.1.20060824090747.01b39ca0@gemini.msu.montana.edu> This has been answered on Histonet several times, just go to Archives and type in F4/80. Barb Wright is one who has given good info on using this antibody from Serotec. There will be other excellent replies also. At 02:18 AM 8/24/2006, you wrote: > I would be interested in hearing from anyone who has used Sertec F4/80 > to do IHC for microglia in pfa fixed mouse brains. I have lovely > staining in my kupfer cells (control) but only staining of what > appears to be neurones in the brain. A protocol would be greatly > appreciated. > > Thanks in advance > > Helen >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From jestrong <@t> comcast.net Thu Aug 24 11:01:40 2006 From: jestrong <@t> comcast.net (Jes Strong) Date: Thu Aug 24 11:01:51 2006 Subject: [Histonet] Refurbished instruments with CMD In-Reply-To: <20060824131831.20959.qmail@web37614.mail.mud.yahoo.com> Message-ID: <003001c6c796$96c2abc0$0200a8c0@Jes> This guy just doesn't quit! I would hope that all Histonetters would boycott supporting his company until he stops his blatant misuse of the Histonet for personal marketing purposes. There are many very good companies out there that respect the intent of the Histonet and can provide you with quality products at competitive prices. Jes Strong -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Coleman Manufacturing Sent: Thursday, August 24, 2006 8:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Refurbished instruments with CMD Tto provide every laboratory and hospital with the opportunity to enjoy the many benefits of our products, CMD is delighted to offer factory-refurbished instruments at significantly discounted prices. All of these instruments are backed by a full one-year parts and service warranty. Offer subject to availability. Equipment of the following types: Cover Slippers Cryostats Microtomes Embedding centers Tissue Processors Stainers Laboratory Microwave Systems You can request more information on the available equipment by contacting our office at: Coleman Manufacturing & Design 2852 Old Airport Road Winnsboro, SC 29180 803.633.2124 office 803.635.9401 fax Coleman_Manufacturing@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Thu Aug 24 11:05:32 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Aug 24 11:05:39 2006 Subject: [Histonet] Cost of consumables In-Reply-To: <082320062209.25134.44ECD214000AC3560000622E2213575333CD9B0C0A009D0A9F0C029B@comcast.net> Message-ID: <20060824160532.39614.qmail@web61212.mail.yahoo.com> Carolyn: I just finished a paper on costs that I am sending you under separate cover. The cost are per unit, just multiply them by your needs. Ren? J. tncperfect2@comcast.net wrote: Hi Histonetters. I need to know how much managers are spending on consumables that deal with 1000 or more specimens a month...give or take a few dollars? I just need a ball park figure for now. We do just the H&E routine stain. If anyone can help me it would be very much appreciated. Thanks, Carolyn _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail Beta. From dcarter <@t> bios-europe.com Thu Aug 24 11:37:41 2006 From: dcarter <@t> bios-europe.com (David Carter) Date: Thu Aug 24 11:31:57 2006 Subject: [Histonet] Refurbished instruments with CMD In-Reply-To: <003001c6c796$96c2abc0$0200a8c0@Jes> Message-ID: <16353168730665@cyserv8.bios-europe.com> Well said The histonet is for help and technical advice Best wishes David Mr D J Carter CSci FIBMS DMS UK Sales and Marketing Manager Bios Europe Ltd Unit 11 Pit Hey Place West Pimbo Skelmersdale Lancashire WN8 9PS Mobile 07795 395538 Tel +44 (0) 1695 555581 fax +44 (0) 0870 1991748 General Enquiries info@bios-europe.com UK Sales Enquires sales@bios-europe.com International Sales Enquiries export@bios-europe.com www.bios-europe.com IMPORTANT PLEASE READ This email may contain confidential or legally privileged information. If you are not the intended recipient you must not use, copy, distribute or disclose the contents. Please immediately notify the sender by reply email and permanently delete this message and its attachments, along with any copies thereof. Any opinions or views expressed in this email are those of the individual sender and do not necessarily represent those of Bios Europe Ltd. This email has been scanned for viruses, vandals and malicious content, but no warranty is given that this email and any attachments are virus free. You should undertake your own virus checking. The right to monitor email communications through our networks is reserved by us. Please note that emails sent to and from Bios Europe are routinely monitored for record keeping, quality control and to ensure compliance with our policies and procedures. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jes Strong Sent: 24 August 2006 17:02 To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Refurbished instruments with CMD This guy just doesn't quit! I would hope that all Histonetters would boycott supporting his company until he stops his blatant misuse of the Histonet for personal marketing purposes. There are many very good companies out there that respect the intent of the Histonet and can provide you with quality products at competitive prices. Jes Strong -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Coleman Manufacturing Sent: Thursday, August 24, 2006 8:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Refurbished instruments with CMD Tto provide every laboratory and hospital with the opportunity to enjoy the many benefits of our products, CMD is delighted to offer factory-refurbished instruments at significantly discounted prices. All of these instruments are backed by a full one-year parts and service warranty. Offer subject to availability. Equipment of the following types: Cover Slippers Cryostats Microtomes Embedding centers Tissue Processors Stainers Laboratory Microwave Systems You can request more information on the available equipment by contacting our office at: Coleman Manufacturing & Design 2852 Old Airport Road Winnsboro, SC 29180 803.633.2124 office 803.635.9401 fax Coleman_Manufacturing@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From dw18 <@t> uchicago.edu Thu Aug 24 12:12:47 2006 From: dw18 <@t> uchicago.edu (David A. Wright) Date: Thu Aug 24 12:12:56 2006 Subject: [Histonet] Re: Super/Frost Plus slides & wrinkled brain sections Message-ID: <20060824121247.ACE40468@m4500-03.uchicago.edu> Hello All I suppose it would be pointless to enjoin those who use Super/Frost Plus slides to actually read the Instructions included with every packet (I too get mine from Fisher Scientific)? Miles Cunningham (via Charles Scouten) remarks that "Superfrost isn't good enough for adherence for free-floating sections at ~30 microns". Not "good enough" is perhaps more suited to the procedure used by Neil Fournier - someone has told him to mount from 0.1M PB. I routinely mount 40um thick frozen-cut & floating-processed rat brain sections to Super/Frost Plus slides without any problems. Guess what? I follow the instructions. I quote: "Float... sections on... distilled water. DO NOT add glue or subbing solution... You may substitute tap water for distilled... but if you begin to use tissue sections, use distilled water." Miles did note that adherence of thaw-mounted sections is better than after immersion in buffer. But why the need for buffer any way? Salts in any buffer will compete preferentially for charged sites on the treated glass (likewise after APES treatment) over proteins in the section, so that the latter cannot bond well. After, say, a floating DAB reaction, I dip each section in a petri dish of 'house' deionized water for a few seconds, rinsing out the brush at the same time, and mount to Super/Frost in this water. (Brain sections should be rinsed individually and not left too long or differential (osmotic?) swelling of various regions can add to the wrinkling problem.) I DO wick away excess water (with Whatman 3MM paper) in order to prevent overlap of e.g. the hippocampus or ventricular margins, since I'm interested in these areas, and find that, if anything, it improves bonding to have as little water and ion traces as possible. I guess the other question is whether the "wrinkling" Neil is really detachment. If it is a shrinkage problem from dehydrating, and he is not doing a counterstain (e.g. Nissl), I suggest that he takes his thoroughly air-dried slides and go directly to a water tolerant clearing agent such as CitriSolv & then resin. For most neurobiological studies, subcellular detail is less important than the distribution & number of different cell types. I find this perfectly acceptable for the markers he describes - NeuN , Brdu etc. All the best -David A. Wright, PhD Section of Neurosurgery University of Chicago From drmichaelryder <@t> yahoo.com Thu Aug 24 13:22:13 2006 From: drmichaelryder <@t> yahoo.com (Michael Ryder) Date: Thu Aug 24 13:22:17 2006 Subject: [Histonet] Re: Histonet Digest, Vol 33, Issue 31 Message-ID: <20060824182213.89125.qmail@web57211.mail.re3.yahoo.com> Good afternoon all ! Just to share with you. We used to purchase our Simport histosettes from major distributors like Fisherbrand and VWR. We just found out a Simport distributor 70% cheaper than them. I dont do publicity (its the only time, promise!)but take 20 sec to check www.econo-lab.com. Same product ! Mike --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. From potianju <@t> umdnj.edu Thu Aug 24 13:23:41 2006 From: potianju <@t> umdnj.edu (Julius A. Potian) Date: Thu Aug 24 13:34:52 2006 Subject: [Histonet] CD4/CD8 staining in murine lungs Message-ID: <006501c6c7aa$6d0db360$a904040a@Goten> Hi, I am new to the world of Frozen section IHC and am trying to standardize a protocol for cd8/cd4 staaing in murine lung tissues embedded in OCT. I have tried without success to stain these sections with primary antibodies from biolegend, secondary antibody biotinylted anti-rat IgG(H+L) from vector labs. I was wondering if anybody has recomendations on a protocol or better antibodies than the one I am using. Also, in developing my slides with DAB (biogenex), sometimes my whole tissue sections turns brown within the matter of seconds. I have done through washes to remove any unbound antibody. I don't know why or where this is occuring. Is it in my snap freezing, sectioning, or staining? Thanks in advance. From RJLevier <@t> LancasterGeneral.org Thu Aug 24 13:44:13 2006 From: RJLevier <@t> LancasterGeneral.org (LeVier, Rebecca J) Date: Thu Aug 24 13:44:20 2006 Subject: [Histonet] (no subject) Message-ID: <0FDBF29200C637468CCC1F9E7E85A3D2D6E027@MAIL-LR.lha.org> Which is the best Glass Coverslipper? Thanks. Rebecca J. LeVier HT(ASCP) Histology Supervisor Phone: (717)-544-5065 Fax: (717)-544-5457 Email: rjlevier@lancastergeneral.org Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From ElizabethWyand <@t> texashealth.org Thu Aug 24 13:53:34 2006 From: ElizabethWyand <@t> texashealth.org (Wyand, Elizabeth) Date: Thu Aug 24 13:53:38 2006 Subject: [Histonet] On-line Histology Schools Message-ID: <26BE9ACC202D29479B0A144066A733E501762A4C@phdex01.txhealth.org> There is another histo school online... it's Harford community college in MD. It's a good program. And, the one at Indiana Univ still offers their via distance, but I don't know if it's online or still teleconference. Elizabeth Wyand Administrator MD Pathology 6124 West Parker Road, Suite G36 Plano, Texas 75093 972.981.3110 direct 972.981.8469 fax 972.981.3107 main office line The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. From Allison_Scott <@t> hchd.tmc.edu Thu Aug 24 14:06:59 2006 From: Allison_Scott <@t> hchd.tmc.edu (Scott, Allison D) Date: Thu Aug 24 14:07:06 2006 Subject: [Histonet] Wearing gloves while cutting sections Message-ID: <1872B4A455B7974391609AD8034C79FC06707D@LBEXCH01.hchd.local> While I was on vaction, the safety committee came around and did rounds in the lab. They sited us for not wearing gloves while cutting sections. My techs tried to explain to them that we did not have to wear gloves, only when cutting frozen sections or while handling fresh tissue. I have never worn gloves while cutting paraffin sections. Now my manager wants me to write a policy in regards to not wearing gloves. Does anyone have anything like this in place that they would be willing to share with me. Your help would be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. From rjbuesa <@t> yahoo.com Thu Aug 24 14:27:13 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Aug 24 14:27:22 2006 Subject: [Histonet] (no subject) In-Reply-To: <0FDBF29200C637468CCC1F9E7E85A3D2D6E027@MAIL-LR.lha.org> Message-ID: <20060824192713.35478.qmail@web61221.mail.yahoo.com> For me it is the Sakura-Finetek. Ren? J. "LeVier, Rebecca J" wrote: Which is the best Glass Coverslipper? Thanks. Rebecca J. LeVier HT(ASCP) Histology Supervisor Phone: (717)-544-5065 Fax: (717)-544-5457 Email: rjlevier@lancastergeneral.org Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your email and more, right on the new Yahoo.com From bhewlett <@t> cogeco.ca Thu Aug 24 14:28:51 2006 From: bhewlett <@t> cogeco.ca (Bryan Hewlett) Date: Thu Aug 24 14:29:03 2006 Subject: [Histonet] Wearing gloves while cutting sections References: <1872B4A455B7974391609AD8034C79FC06707D@LBEXCH01.hchd.local> Message-ID: <007201c6c7b3$8782d690$6500a8c0@mainbox> What was the rationale behind them citing you for not wearing gloves? Bryan ----- Original Message ----- From: "Scott, Allison D" To: Sent: Thursday, August 24, 2006 3:06 PM Subject: [Histonet] Wearing gloves while cutting sections While I was on vaction, the safety committee came around and did rounds in the lab. They sited us for not wearing gloves while cutting sections. My techs tried to explain to them that we did not have to wear gloves, only when cutting frozen sections or while handling fresh tissue. I have never worn gloves while cutting paraffin sections. Now my manager wants me to write a policy in regards to not wearing gloves. Does anyone have anything like this in place that they would be willing to share with me. Your help would be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From froyer <@t> bitstream.net Thu Aug 24 14:30:56 2006 From: froyer <@t> bitstream.net (Ford Royer) Date: Thu Aug 24 14:31:05 2006 Subject: [Histonet] Refurbished instruments with CMD In-Reply-To: <20060824131831.20959.qmail@web37614.mail.mud.yahoo.com> Message-ID: <000c01c6c7b3$d25fe090$7701a80a@Ford> In the early days of the HistoNet there were "Netiquette" rules posted in the welcoming instructions to subscribers of the HistoNet. I have long since deleted my original copy but, if it is still available, perhaps someone could forward these rules to the good people at CMD for their review and adherence... especially the clause stating that the HistoNet is not to be used for blatant advertising by commercial vendors. Hopefully this can be done before all vendors are banned from the HistoNet due to the actions of one. ~ Ford Ford M. Royer, MT(ASCP) Minnesota Medical, Inc. 7177 Madison Ave. W. Golden Valley, MN 55427-3601 CELL: 612-839-1046 Phone: 763-542-8725 Fax: 763-546-4830 eMail: froyer@bitstream.net -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Coleman Manufacturing Sent: Thursday, August 24, 2006 8:19 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Refurbished instruments with CMD Tto provide every laboratory and hospital with the opportunity to enjoy the many benefits of our products, CMD is delighted to offer factory-refurbished instruments at significantly discounted prices. All of these instruments are backed by a full one-year parts and service warranty. Offer subject to availability. Equipment of the following types: Cover Slippers Cryostats Microtomes Embedding centers Tissue Processors Stainers Laboratory Microwave Systems You can request more information on the available equipment by contacting our office at: Coleman Manufacturing & Design 2852 Old Airport Road Winnsboro, SC 29180 803.633.2124 office 803.635.9401 fax Coleman_Manufacturing@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From GDawson <@t> dynacaremilwaukee.com Thu Aug 24 14:36:53 2006 From: GDawson <@t> dynacaremilwaukee.com (Dawson, Glen) Date: Thu Aug 24 14:37:13 2006 Subject: [Histonet] (no subject) Message-ID: The Sakura Tissue-Tek Glas. Glen Dawson Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of LeVier, Rebecca J Sent: Thursday, August 24, 2006 12:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Which is the best Glass Coverslipper? Thanks. Rebecca J. LeVier HT(ASCP) Histology Supervisor Phone: (717)-544-5065 Fax: (717)-544-5457 Email: rjlevier@lancastergeneral.org Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From RJLevier <@t> LancasterGeneral.org Thu Aug 24 14:42:13 2006 From: RJLevier <@t> LancasterGeneral.org (LeVier, Rebecca J) Date: Thu Aug 24 14:42:19 2006 Subject: [Histonet] Wearing gloves while cutting sections Message-ID: <0FDBF29200C637468CCC1F9E7E85A3D2D6E02A@MAIL-LR.lha.org> I am not sure that the committee fully understood what the process was. Could they have been looking at the wrong thing. Did they really mean to site for this or were they just having a misunderstanding? If it were me, I would try to get together with them to talk. For me, it is almost impossible for me to grab a ribbon with gloves on. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Thursday, August 24, 2006 3:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Wearing gloves while cutting sections While I was on vaction, the safety committee came around and did rounds in the lab. They sited us for not wearing gloves while cutting sections. My techs tried to explain to them that we did not have to wear gloves, only when cutting frozen sections or while handling fresh tissue. I have never worn gloves while cutting paraffin sections. Now my manager wants me to write a policy in regards to not wearing gloves. Does anyone have anything like this in place that they would be willing to share with me. Your help would be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. From RohrT <@t> nyackhospital.org Thu Aug 24 14:42:11 2006 From: RohrT <@t> nyackhospital.org (Theresa Rohr) Date: Thu Aug 24 14:46:09 2006 Subject: [Histonet] Silanized Slides Message-ID: Dear Histonetters, I have been using Dako Silanized slides for my IHC successfully. Problem is they are exorbitantly expensive and lately their Customer Service has fallen below par. I need another source for a similar slide. I definitely do not want to make my own. Too few staff, too much work. If you or any vendors out there have a similar product, please let me know. I am swamped and could use the help with the research. Thanks so much, Theresa Rohr, Nyack Hospital NY Theresa Rohr, BA, HT(ASCP) Section Head, Histology Nyack Hospital 160 North Midland Avenue Nyack, New York 10960 phone 845-348-2276 fax 845-348-8430 Nyack Hospital is required by Law to protect the privacy of health information under HIPAA Rules and Regulations Part 11, Article 45 CFR, Parts 160-164. This email, including attachments, is intended for the exclusive use of the person or the entity to which it is addressed. It may contain confidential or legally protected information. The authorized recipient is prohibited from disclosing this information to any other party and is required to destroy this information after its stated need has been fulfilled. If the recipient or reader of this email is not the intended recipient or her/his authorized agent, the reader is hereby notified that any dissemination, distribution or copying of this email is prohibited. If you believe that you have received this email in error, please advise the sender immediately by reply email and then delete this email immediately. From rjbuesa <@t> yahoo.com Thu Aug 24 15:17:36 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Aug 24 15:17:39 2006 Subject: [Histonet] Silanized Slides In-Reply-To: Message-ID: <20060824201736.42280.qmail@web61216.mail.yahoo.com> By the chemical product from Sigma and silanize your own slides. Ren? J. Theresa Rohr wrote: Dear Histonetters, I have been using Dako Silanized slides for my IHC successfully. Problem is they are exorbitantly expensive and lately their Customer Service has fallen below par. I need another source for a similar slide. I definitely do not want to make my own. Too few staff, too much work. If you or any vendors out there have a similar product, please let me know. I am swamped and could use the help with the research. Thanks so much, Theresa Rohr, Nyack Hospital NY Theresa Rohr, BA, HT(ASCP) Section Head, Histology Nyack Hospital 160 North Midland Avenue Nyack, New York 10960 phone 845-348-2276 fax 845-348-8430 Nyack Hospital is required by Law to protect the privacy of health information under HIPAA Rules and Regulations Part 11, Article 45 CFR, Parts 160-164. This email, including attachments, is intended for the exclusive use of the person or the entity to which it is addressed. It may contain confidential or legally protected information. The authorized recipient is prohibited from disclosing this information to any other party and is required to destroy this information after its stated need has been fulfilled. If the recipient or reader of this email is not the intended recipient or her/his authorized agent, the reader is hereby notified that any dissemination, distribution or copying of this email is prohibited. If you believe that you have received this email in error, please advise the sender immediately by reply email and then delete this email immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. From rjbuesa <@t> yahoo.com Thu Aug 24 15:21:58 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Aug 24 15:22:01 2006 Subject: [Histonet] Wearing gloves while cutting sections In-Reply-To: <0FDBF29200C637468CCC1F9E7E85A3D2D6E02A@MAIL-LR.lha.org> Message-ID: <20060824202158.92227.qmail@web61217.mail.yahoo.com> Rebecca: I think that you should expalin your thoughtful and illustrious commettee that once the tissue is fixed and processed (unless it is KJ disease case) you can lick the block. By the way, I once did just that in front of the safety officer of our lab (she left me alone for ever. I still don't know if she left me alone because she was convinced or because she thought that I was completely out of my mind; it worked!). Ren? J. "LeVier, Rebecca J" wrote: I am not sure that the committee fully understood what the process was. Could they have been looking at the wrong thing. Did they really mean to site for this or were they just having a misunderstanding? If it were me, I would try to get together with them to talk. For me, it is almost impossible for me to grab a ribbon with gloves on. -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Thursday, August 24, 2006 3:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Wearing gloves while cutting sections While I was on vaction, the safety committee came around and did rounds in the lab. They sited us for not wearing gloves while cutting sections. My techs tried to explain to them that we did not have to wear gloves, only when cutting frozen sections or while handling fresh tissue. I have never worn gloves while cutting paraffin sections. Now my manager wants me to write a policy in regards to not wearing gloves. Does anyone have anything like this in place that they would be willing to share with me. Your help would be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your own web address for just $1.99/1st yr. We'll help. Yahoo! Small Business. From HornHV <@t> archildrens.org Thu Aug 24 15:28:24 2006 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Aug 24 15:28:42 2006 Subject: [Histonet] Wearing gloves while cutting sections In-Reply-To: <1872B4A455B7974391609AD8034C79FC06707D@LBEXCH01.hchd.local> Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6D5A@EMAIL.archildrens.org> We actually have a policy on when and where gloves are to be worn for the whole laboratory. (clinical and anatomic) It's crazy. In our lab, it is personal preference while cutting. I have one tech who wears gloves (he was a med tech) and the other tech and I do not. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D Sent: Thursday, August 24, 2006 2:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Wearing gloves while cutting sections While I was on vaction, the safety committee came around and did rounds in the lab. They sited us for not wearing gloves while cutting sections. My techs tried to explain to them that we did not have to wear gloves, only when cutting frozen sections or while handling fresh tissue. I have never worn gloves while cutting paraffin sections. Now my manager wants me to write a policy in regards to not wearing gloves. Does anyone have anything like this in place that they would be willing to share with me. Your help would be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From HornHV <@t> archildrens.org Thu Aug 24 15:30:17 2006 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Thu Aug 24 15:30:34 2006 Subject: [Histonet] would someone from sakura contact me? Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6D5B@EMAIL.archildrens.org> Would someone from Sakura please contact me? Thanks. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From liz <@t> premierlab.com Thu Aug 24 16:04:59 2006 From: liz <@t> premierlab.com (Liz Chlipala) Date: Thu Aug 24 16:00:05 2006 Subject: [Histonet] Silanized Slides In-Reply-To: <20060824201736.42280.qmail@web61216.mail.yahoo.com> Message-ID: <000e01c6c7c0$f5c58a50$0300a8c0@domain.Premier> I use two slides for IHC one I get from Mercedes medical they are the platinum slides, I'm convinced that these are the same slides that sigma sells as their Silane Prep Slides, they are very reasonably priced I use these for all soft tissue IHC and never have issues. For bone I use Histobond slides from Statlab, they are a bit more expensive but work well for all of the IHC on bone we do. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Thursday, August 24, 2006 2:18 PM To: Theresa Rohr; Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Silanized Slides By the chemical product from Sigma and silanize your own slides. Ren? J. Theresa Rohr wrote: Dear Histonetters, I have been using Dako Silanized slides for my IHC successfully. Problem is they are exorbitantly expensive and lately their Customer Service has fallen below par. I need another source for a similar slide. I definitely do not want to make my own. Too few staff, too much work. If you or any vendors out there have a similar product, please let me know. I am swamped and could use the help with the research. Thanks so much, Theresa Rohr, Nyack Hospital NY Theresa Rohr, BA, HT(ASCP) Section Head, Histology Nyack Hospital 160 North Midland Avenue Nyack, New York 10960 phone 845-348-2276 fax 845-348-8430 Nyack Hospital is required by Law to protect the privacy of health information under HIPAA Rules and Regulations Part 11, Article 45 CFR, Parts 160-164. This email, including attachments, is intended for the exclusive use of the person or the entity to which it is addressed. It may contain confidential or legally protected information. The authorized recipient is prohibited from disclosing this information to any other party and is required to destroy this information after its stated need has been fulfilled. If the recipient or reader of this email is not the intended recipient or her/his authorized agent, the reader is hereby notified that any dissemination, distribution or copying of this email is prohibited. If you believe that you have received this email in error, please advise the sender immediately by reply email and then delete this email immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1?/min. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Eric <@t> ategra.com Thu Aug 24 11:48:56 2006 From: Eric <@t> ategra.com (Eric Dye (ext 223)) Date: Thu Aug 24 16:03:25 2006 Subject: [Histonet] Histology Perm and Temp J O B S in your area Message-ID: Hi - Fellow-Histonetters Are you still working in Histology at ? Below is the updated list of both temp and perm Histology j o b s, All openings are Dayshift Monday thru Friday unless indicated otherwise. All of these clients are currently looking to move quickly so if you are interested call me A S A P at 800-466-9919 ext 223 or cell - 407-756-5507. Most HistoTech j o b s are permanent full-time unless indicated otherwise Permanent Histology Jobs: ------------------------------------------------------ Ohio (Southern) - perm - Bench Histotech ( 2 openings) Washington, D.C - Histotech -perm and temp Northern New Jersey - HistoTech - perm Boston Mass - HistoTech - one Senior HistoTech, One not so Senior Histotech Boston Mass - HistoTech - HistoTech -perm Massachusetts (North of Boston) - perm - Bench Histotech Central Florida -perm- Histotech Southeast Florida - perm - HistoTech Southeast Florida - perm - HistoTech Southwest Florida - perm - Bench Histotech/ Supervisor Florida, West Coast - both temp & perm openings- Bench Histotech New Hampshire perm openings - Bench Histotech (opportunity to learn Mohs!) -----------------------------------end perm jobs ---------------------------------------------------- Temporary Assignments ------------------------------- Alaska -Histo Tech Temp - minimum of 16 weeks Washington,DC - HistoTech - minimum of 6 weeks- also Temp to Perm Massachusetts (Boston area) - Histo Tech minimum of 13 weeks (2 people) -------------------------- end list of HistoTech Opportunities ------------------------------------- If you are interested in any of the Histology jobs listed above - please call me at 800-466-9919 ext 223 or cell - 407-756-5507. Thank you, Eric Dye-Sr Allied Healthcare Recruiter 800-466-9919 ext 223 or Cell - 407-756-5507 P.S.: Feel free to pass along this email and my phone number to anyone who you think might be interested. P.S.S.: The clients are currently interviewing - and the job will close soon - so if you are interested, please call me today at 1-800-466-9919 ext 229 --------------------------------------------------------------- Ategra Systems Inc Specialists in Permanent & Contract Staffing 800 466 9919 ext 223 - voice 407 671 6075 - fax [1]eric@ategra.com To Learn More About Ategra: [2]http://www.ategra.com ---------------------------------------------------------------- If you received this by mistake, or if you wish not to hear from me, please shoot me a mail to let me know and I'll not mail you again. ---------------------------------------------------------------- ---------------------------------------------------------------- Note: this message is intended for: Fellow-Histonetters at histonet@lists.utsouthwestern.edu ---------------------------------------------------------------- References 1. mailto:eric@ategra.com 2. http://www.ategra.com/ From Janet.Bonner <@t> FLHOSP.ORG Thu Aug 24 16:02:38 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Thu Aug 24 16:04:57 2006 Subject: [Histonet] Wearing gloves while cutting sections References: <1872B4A455B7974391609AD8034C79FC06707D@LBEXCH01.hchd.local> <007201c6c7b3$8782d690$6500a8c0@mainbox> Message-ID: With that mentality, we'd all be dressed up like Astronauts!!! ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of Bryan Hewlett Sent: Thu 8/24/2006 3:28 PM To: Scott, Allison D; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Wearing gloves while cutting sections What was the rationale behind them citing you for not wearing gloves? Bryan ----- Original Message ----- From: "Scott, Allison D" To: Sent: Thursday, August 24, 2006 3:06 PM Subject: [Histonet] Wearing gloves while cutting sections While I was on vaction, the safety committee came around and did rounds in the lab. They sited us for not wearing gloves while cutting sections. My techs tried to explain to them that we did not have to wear gloves, only when cutting frozen sections or while handling fresh tissue. I have never worn gloves while cutting paraffin sections. Now my manager wants me to write a policy in regards to not wearing gloves. Does anyone have anything like this in place that they would be willing to share with me. Your help would be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From TJasper <@t> smdc.org Thu Aug 24 16:14:23 2006 From: TJasper <@t> smdc.org (Jasper, Thomas G.) Date: Thu Aug 24 16:14:44 2006 Subject: [Histonet] Wearing gloves while cutting sections In-Reply-To: <1872B4A455B7974391609AD8034C79FC06707D@LBEXCH01.hchd.local> Message-ID: <1A9F2A6C5762524799A816F1F09744CF1E0B7B@SCREECH.ntcampus.smdc.org> Hey Allison, It sounds like your safety committee members thought they could cite you based on a policy that is in place. If that is indeed the case, I would take that policy and put an addendum onto it. You would need only write a couple of sentences or a paragraph to note the exemption from gloving that exists for histotechs when cutting sections. In your policy manual you would just insert the addendum after the main body of information for that policy. You could then make a note on a revision page referencing the addendum, date and initial it. I would think that your pathologists or lab director would be supportive of such a move as it is direct and uncomplicated. I personally favor keeping bureaucracy to a minimum when possible. I think this solution is preferrable to writing a whole new policy based on a minor issue which is standard and acceptable practice in clinical AP settings to my knowledge. Good luck, hope this helps. Thomas Jasper AP Supervisor SMDC Duluth, MN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Scott, Allison D Sent: Thursday, August 24, 2006 2:07 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Wearing gloves while cutting sections While I was on vaction, the safety committee came around and did rounds in the lab. They sited us for not wearing gloves while cutting sections. My techs tried to explain to them that we did not have to wear gloves, only when cutting frozen sections or while handling fresh tissue. I have never worn gloves while cutting paraffin sections. Now my manager wants me to write a policy in regards to not wearing gloves. Does anyone have anything like this in place that they would be willing to share with me. Your help would be greatly appreciated. Allison Scott HT(ASCP) Histology Supervisor LBJ Hospital Houston, Texas 77026 CONFIDENTIALITY NOTICE: If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail and any attachments from your computer system. To the extent the information in this e-mail and any attachments contain protected health information as defined by the Health Insurance Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is confidential and/or privileged. This e-mail may also be confidential and/or privileged under Texas law. The e-mail is for the use of only the individual or entity named above. If you are not the intended recipient, or any authorized representative of the intended recipient, you are hereby notified that any review, dissemination or copying of this e-mail and its attachments is strictly prohibited. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From gcallis <@t> montana.edu Thu Aug 24 17:16:15 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Aug 24 17:16:21 2006 Subject: [Histonet] CD4/CD8 staining in murine lungs In-Reply-To: <006501c6c7aa$6d0db360$a904040a@Goten> References: <006501c6c7aa$6d0db360$a904040a@Goten> Message-ID: <6.0.0.22.1.20060824160243.01b81dc8@gemini.msu.montana.edu> Julius, Our lung speciality laboratory does not use peroxidase methods with lung, they get too much background even with endog peroxidase blocking, they prefer AP method instead and with Permanent Red from DAKO, superb, sensitive and great contrast with hematoxylin NBT/BCIP, as sensitive as DAB, but use nuclear fast red as the counterstain with this one. TBS is the buffer with 0.05% Tween 20 for AP method. We fill lung with OCT, cut 5 um frozens, fix with acetone/alcohol (I will be glad to provide more detail on this fixative, but it is also already in Histonet archives many times) The CD4 and CD8 clones come from BD Pharmingen, and we only use either goat or Donkey F(ab')2 frag of IgG anti Rat -biotin, adsorbed to mouse,with these antibodies to avoid cross reaction to fc receptors and between closely related species. We also have superb results when both of these antibodies are already biotinylated. then come back with Strepavidin-HRP, no secondary and less time. We also use a 10% goat or Donkey serum (depends on host of secondary used) with 2.5% mouse serum as the normal serum block 30 min, and use this block as the diluent for the secondary antibody OR for a biotinylated Rat antiMouse CD4 or CD8, trust me, it works. We prefer to do our own inhouse dilutions of Strepavidin-HRP or AP from Biosource, although Southern Biotechnology has excellent reagent, 0.5 - 0.6 mg/ml diluted 1:500, for 20 min Be sure you develop your DAB using microscope to control overdevelopment and do a dilution panel for both antibodies starting at 10 ug/ml. Our CD4 is at 1:500 or so, a 0.5mg/ml conc, and the CD8 is 1:200, always the weakest of the two. Different chromogens wil have different conc of primary, so be sure to do the dilution panel. Secondaries are generally 2 - 5 ug/ml. At 12:23 PM 8/24/2006, you wrote: >Hi, > I am new to the world of Frozen section IHC and am trying to > standardize a protocol for cd8/cd4 staaing in murine lung tissues > embedded in OCT. I have tried without success to stain these sections > with primary antibodies from biolegend, secondary antibody biotinylted > anti-rat IgG(H+L) from vector labs. I was wondering if anybody has > recomendations on a protocol or better antibodies than the one I am > using. Also, in developing my slides with DAB (biogenex), sometimes my > whole tissue sections turns brown within the matter of seconds. I have > done through washes to remove any unbound antibody. I don't know why or > where this is occuring. Is it in my snap freezing, sectioning, or > staining? Thanks in advance. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From gcallis <@t> montana.edu Thu Aug 24 17:26:11 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Thu Aug 24 17:26:15 2006 Subject: [Histonet] Wearing gloves while cutting sections In-Reply-To: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6D5A@EMAIL.archildrens .org> References: <1872B4A455B7974391609AD8034C79FC06707D@LBEXCH01.hchd.local> <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6D5A@EMAIL.archildrens.org> Message-ID: <6.0.0.22.1.20060824161917.01b700c0@gemini.msu.montana.edu> Even in the research setting, we wear gloves for all cryotomy and all prion frozen and paraffin work, otherwise no gloves for paraffin. One eventually learns to grasp a ribbon. We use forceps when wearing gloves for paraffin ribbon - a learning curve, but I now use forceps for all paraffin work exclucsively. I suggest using nitrile gloves over latex - but find one that fits nicely without cutting circulation to hands and fingers! Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From jnocito <@t> satx.rr.com Thu Aug 24 18:42:04 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Thu Aug 24 18:42:15 2006 Subject: [Histonet] Wearing gloves while cutting sections References: <20060824202158.92227.qmail@web61217.mail.yahoo.com> Message-ID: <001c01c6c7d6$e7da73e0$4a624542@yourxhtr8hvc4p> Lick the block!? Damn, I thought I was the only one doing that. I used to do that with Pap smears. "This one is negative, negative, negative, positive, negative, oooh, this has HPV". Y'all didn't think I'd let this go by without a comment, did you? I love Fridays!!! Ooops, it's only Thursday. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Rene J Buesa" To: "LeVier, Rebecca J" ; "Scott, Allison D" ; Sent: Thursday, August 24, 2006 3:21 PM Subject: RE: [Histonet] Wearing gloves while cutting sections > Rebecca: > I think that you should expalin your thoughtful and illustrious commettee > that once the tissue is fixed and processed (unless it is KJ disease case) > you can lick the block. > By the way, I once did just that in front of the safety officer of our > lab (she left me alone for ever. I still don't know if she left me alone > because she was convinced or because she thought that I was completely out > of my mind; it worked!). > Ren? J. > > "LeVier, Rebecca J" wrote: > I am not sure that the committee fully understood what the process was. > Could they have been looking at the wrong thing. Did they really mean to > site for this or were they just having a misunderstanding? If it were > me, I would try to get together with them to talk. For me, it is almost > impossible for me to grab a ribbon with gloves on. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, > Allison D > Sent: Thursday, August 24, 2006 3:07 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Wearing gloves while cutting sections > > While I was on vaction, the safety committee came around and did rounds > in the lab. They sited us for not wearing gloves while cutting > sections. My techs tried to explain to them that we did not have to > wear gloves, only when cutting frozen sections or while handling fresh > tissue. I have never worn gloves while cutting paraffin sections. Now > my manager wants me to write a policy in regards to not wearing gloves. > Does anyone have anything like this in place that they would be willing > to share with me. Your help would be greatly appreciated. > > Allison Scott HT(ASCP) > Histology Supervisor > LBJ Hospital > Houston, Texas 77026 > > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify the > sender by return e-mail and delete this e-mail and any attachments from > your computer system. > > To the extent the information in this e-mail and any attachments contain > protected health information as defined by the Health Insurance > Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR > Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is > confidential and/or privileged. This e-mail may also be confidential > and/or privileged under Texas law. The e-mail is for the use of only > the individual or entity named above. If you are not the intended > recipient, or any authorized representative of the intended recipient, > you are hereby notified that any review, dissemination or copying of > this e-mail and its attachments is strictly prohibited. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of intended recipient(s) and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by reply e-mail and > destroy all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Get your own web address for just $1.99/1st yr. We'll help. Yahoo! Small > Business. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.405 / Virus Database: 268.11.5/426 - Release Date: 8/23/2006 > > From tahseen <@t> brain.net.pk Thu Aug 24 20:26:02 2006 From: tahseen <@t> brain.net.pk (Tahseen) Date: Thu Aug 24 20:26:15 2006 Subject: [Histonet] Frozens section cutinng by resident Dr Message-ID: <013f01c6c7e5$6f396b30$131e80cb@p> Hi every body Are there any other hospitals out there where the pathologists or resident cut frozen section stain and mount their own cases? Muhammad Tahseen From hawkmoon15 <@t> cox.net Thu Aug 24 20:28:28 2006 From: hawkmoon15 <@t> cox.net (Sarah Jones) Date: Thu Aug 24 20:29:12 2006 Subject: [Histonet] On-line Histology Schools References: <26BE9ACC202D29479B0A144066A733E501762A4C@phdex01.txhealth.org> Message-ID: <007801c6c7e5$c5a12b70$91d7c248@SILVERHORSE> I responded to the original requester of this information privately yesterday. I believe she is being misled by statements such as "There is another histo school online... it's Harford community college in MD. It's a good program." I am sure this is a good program. However, one cannot complete this program and take their boards unless they comply with what Peggy Wenk said below: "I feel like I have wandered into the middle of a conversation with this. Don't know what preceded this comment, but will try to answer from this point on. The THEORY of Histotechnology could be taught on line. What educators call the "didactics." Floyd Grimm III, in a NAACLS accredited program at Harford Community College in Bel Air, Maryland has most of his histotechnology lectures on line. The students download the homework, and email the answers back in. They follow links to other sites, and read up on procedures from other institutions. They follow links that Floyd has set up, to look at photos of equipment, and the end results of stains. I think they could also take some practice exams via the internet. HOWEVER, his students still need to come in to the college to do the lab portion of the college courses. And they still need to do their clinical rotations in the area hospital and private labs. So they are still getting hands-on in the college labs and the hospital labs. But they are getting the theory on-line." PLEASE folks, before informing anyone about what you THINK you know, refer them to www.ASCP.org and let them check out all the actual requirements for licensure. So much has changed in the past few years, I doubt all the HT & HTL licensees are even aware of all the changes. Best regards, Sarah A. Jones, HTL(ASCP) Dako North America, Inc. Research Associate (800) 235-5743 ext. 5681 From BMolinari <@t> heart.thi.tmc.edu Fri Aug 25 05:30:15 2006 From: BMolinari <@t> heart.thi.tmc.edu (Molinari, Betsy) Date: Fri Aug 25 05:30:21 2006 Subject: [Histonet] (no subject) In-Reply-To: Message-ID: Sakura Tissue Tek Glas. Betsy Molinari HT (ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. Houston,TX 77030 832-355-6524 832-355-6812 (fax) -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dawson, Glen Sent: Thursday, August 24, 2006 2:37 PM To: LeVier, Rebecca J; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] (no subject) The Sakura Tissue-Tek Glas. Glen Dawson Milwaukee, WI -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of LeVier, Rebecca J Sent: Thursday, August 24, 2006 12:44 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Which is the best Glass Coverslipper? Thanks. Rebecca J. LeVier HT(ASCP) Histology Supervisor Phone: (717)-544-5065 Fax: (717)-544-5457 Email: rjlevier@lancastergeneral.org Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Virginia.Achstetter <@t> afip.osd.mil Fri Aug 25 05:50:32 2006 From: Virginia.Achstetter <@t> afip.osd.mil (Achstetter, Virginia A.) Date: Fri Aug 25 05:50:54 2006 Subject: [Histonet] TMA Book Message-ID: <127D612DBCD77546A12C4746F7491C7F111AD1@lewis.afip.osd.mil> Are there any good books out on how to prepare a tma block, trouble shooting, cutting techniques etc. Ginny Achstetter From Virginia.Achstetter <@t> afip.osd.mil Fri Aug 25 05:50:32 2006 From: Virginia.Achstetter <@t> afip.osd.mil (Achstetter, Virginia A.) Date: Fri Aug 25 05:50:57 2006 Subject: [Histonet] TMA Book Message-ID: <127D612DBCD77546A12C4746F7491C7F111AD1@lewis.afip.osd.mil> Are there any good books out on how to prepare a tma block, trouble shooting, cutting techniques etc. Ginny Achstetter From ree3 <@t> leicester.ac.uk Fri Aug 25 06:28:51 2006 From: ree3 <@t> leicester.ac.uk (Edwards, R.E.) Date: Fri Aug 25 06:28:57 2006 Subject: [Histonet] Choline kinase In-Reply-To: Message-ID: Anybody aware of an antibody to choline kinase that works on paraffin processed mouse tissues?? many thanks. Richard Edwards MRC TOXICOLOGY UNIT LEICESTER..U.K.... From petepath <@t> yahoo.com Fri Aug 25 06:39:11 2006 From: petepath <@t> yahoo.com (Stephen Peters M.D.) Date: Fri Aug 25 06:39:15 2006 Subject: [Histonet] Frozens section cutinng by resident Dr Message-ID: <20060825113911.36537.qmail@web30414.mail.mud.yahoo.com> Dear Muhammad, Pathologists and residents here at HUMC pathologists, prepare our own frozens. Our residents from UMDNJ do the same here and at University hospital. Hopefully you will find that residents are still preparing frozens in many of our residency programs. I believe it is a very important part of our training and is a requirement of the American Board of Pathology.Even in hospitals where histologists and PA's prepare the frozens, the pathologists will often find themselves needing these skills in off hours. I have observed a great variability in the training and ability of pathologists at the cryostat. For this reason I offer all I have learned in my experience in a free frozen section tutorial on my web site at : http://pathologyinnovations.com/frozen_section_technique.htm I have also offered this as a workshop at a number of histosociety meetings and will be giving it at the region II meeting on Oct 28. Stephen Stephen Peters M.D. Vice Chairman of Pathology Hackensack University Medical Center 201 996 4836 Pathology Innovations, LLC 410 Old Mill Lane, Wyckoff, NJ 07481 201 847 7600 www.pathologyinnovations.com From RBARNHART <@t> summithealth.org Fri Aug 25 06:45:22 2006 From: RBARNHART <@t> summithealth.org (Rebecca Barnhart) Date: Fri Aug 25 06:46:08 2006 Subject: [Histonet] Wearing gloves while cutting sections Message-ID: Wow! I thought it was weird when I once witnessed a pathologist drink Hank solution to determine if it was similar to a drink. Of course the Hank was fresh. That experience explained a lot about that pathologist. >>> "Joe Nocito" 8/24/2006 7:42:04 PM >>> Lick the block!? Damn, I thought I was the only one doing that. I used to do that with Pap smears. "This one is negative, negative, negative, positive, negative, oooh, this has HPV". Y'all didn't think I'd let this go by without a comment, did you? I love Fridays!!! Ooops, it's only Thursday. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Rene J Buesa" To: "LeVier, Rebecca J" ; "Scott, Allison D" ; Sent: Thursday, August 24, 2006 3:21 PM Subject: RE: [Histonet] Wearing gloves while cutting sections > Rebecca: > I think that you should expalin your thoughtful and illustrious commettee > that once the tissue is fixed and processed (unless it is KJ disease case) > you can lick the block. > By the way, I once did just that in front of the safety officer of our > lab (she left me alone for ever. I still don't know if she left me alone > because she was convinced or because she thought that I was completely out > of my mind; it worked!). > Ren? J. > > "LeVier, Rebecca J" wrote: > I am not sure that the committee fully understood what the process was. > Could they have been looking at the wrong thing. Did they really mean to > site for this or were they just having a misunderstanding? If it were > me, I would try to get together with them to talk. For me, it is almost > impossible for me to grab a ribbon with gloves on. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, > Allison D > Sent: Thursday, August 24, 2006 3:07 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Wearing gloves while cutting sections > > While I was on vaction, the safety committee came around and did rounds > in the lab. They sited us for not wearing gloves while cutting > sections. My techs tried to explain to them that we did not have to > wear gloves, only when cutting frozen sections or while handling fresh > tissue. I have never worn gloves while cutting paraffin sections. Now > my manager wants me to write a policy in regards to not wearing gloves. > Does anyone have anything like this in place that they would be willing > to share with me. Your help would be greatly appreciated. > > Allison Scott HT(ASCP) > Histology Supervisor > LBJ Hospital > Houston, Texas 77026 > > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify the > sender by return e-mail and delete this e-mail and any attachments from > your computer system. > > To the extent the information in this e-mail and any attachments contain > protected health information as defined by the Health Insurance > Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR > Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is > confidential and/or privileged. This e-mail may also be confidential > and/or privileged under Texas law. The e-mail is for the use of only > the individual or entity named above. If you are not the intended > recipient, or any authorized representative of the intended recipient, > you are hereby notified that any review, dissemination or copying of > this e-mail and its attachments is strictly prohibited. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of intended recipient(s) and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by reply e-mail and > destroy all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Get your own web address for just $1.99/1st yr. We'll help. Yahoo! Small > Business. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.405 / Virus Database: 268.11.5/426 - Release Date: 8/23/2006 > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMahoney <@t> alegent.org Fri Aug 25 06:52:02 2006 From: JMahoney <@t> alegent.org (Janice Mahoney) Date: Fri Aug 25 06:52:13 2006 Subject: [Histonet] Wearing gloves while cutting sections Message-ID: I've been in this field for over 30 years and have never heard of Hank solution. Do I want to drink it? And Joe, if anyone out there in Histoland ever questioned whether or not you are a nut, they now know. >>> "Rebecca Barnhart" 08/25/2006 6:45 AM >>> Wow! I thought it was weird when I once witnessed a pathologist drink Hank solution to determine if it was similar to a drink. Of course the Hank was fresh. That experience explained a lot about that pathologist. >>> "Joe Nocito" 8/24/2006 7:42:04 PM >>> Lick the block!? Damn, I thought I was the only one doing that. I used to do that with Pap smears. "This one is negative, negative, negative, positive, negative, oooh, this has HPV". Y'all didn't think I'd let this go by without a comment, did you? I love Fridays!!! Ooops, it's only Thursday. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Rene J Buesa" To: "LeVier, Rebecca J" ; "Scott, Allison D" ; Sent: Thursday, August 24, 2006 3:21 PM Subject: RE: [Histonet] Wearing gloves while cutting sections > Rebecca: > I think that you should expalin your thoughtful and illustrious commettee > that once the tissue is fixed and processed (unless it is KJ disease case) > you can lick the block. > By the way, I once did just that in front of the safety officer of our > lab (she left me alone for ever. I still don't know if she left me alone > because she was convinced or because she thought that I was completely out > of my mind; it worked!). > Ren? J. > > "LeVier, Rebecca J" wrote: > I am not sure that the committee fully understood what the process was. > Could they have been looking at the wrong thing. Did they really mean to > site for this or were they just having a misunderstanding? If it were > me, I would try to get together with them to talk. For me, it is almost > impossible for me to grab a ribbon with gloves on. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, > Allison D > Sent: Thursday, August 24, 2006 3:07 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Wearing gloves while cutting sections > > While I was on vaction, the safety committee came around and did rounds > in the lab. They sited us for not wearing gloves while cutting > sections. My techs tried to explain to them that we did not have to > wear gloves, only when cutting frozen sections or while handling fresh > tissue. I have never worn gloves while cutting paraffin sections. Now > my manager wants me to write a policy in regards to not wearing gloves. > Does anyone have anything like this in place that they would be willing > to share with me. Your help would be greatly appreciated. > > Allison Scott HT(ASCP) > Histology Supervisor > LBJ Hospital > Houston, Texas 77026 > > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify the > sender by return e-mail and delete this e-mail and any attachments from > your computer system. > > To the extent the information in this e-mail and any attachments contain > protected health information as defined by the Health Insurance > Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR > Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is > confidential and/or privileged. This e-mail may also be confidential > and/or privileged under Texas law. The e-mail is for the use of only > the individual or entity named above. If you are not the intended > recipient, or any authorized representative of the intended recipient, > you are hereby notified that any review, dissemination or copying of > this e-mail and its attachments is strictly prohibited. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of intended recipient(s) and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by reply e-mail and > destroy all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Get your own web address for just $1.99/1st yr. We'll help. Yahoo! Small > Business. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.405 / Virus Database: 268.11.5/426 - Release Date: 8/23/2006 > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From JMahoney <@t> alegent.org Fri Aug 25 06:56:05 2006 From: JMahoney <@t> alegent.org (Janice Mahoney) Date: Fri Aug 25 06:57:03 2006 Subject: [Histonet] Wearing gloves while cutting sections Message-ID: I've been in this field for over 30 years and have never heard of Hank solution. Do I want to drink it? And Joe, if anyone out there in Histoland ever questioned whether or not you are a nut, they now know. >>> "Rebecca Barnhart" 08/25/2006 6:45 AM >>> Wow! I thought it was weird when I once witnessed a pathologist drink Hank solution to determine if it was similar to a drink. Of course the Hank was fresh. That experience explained a lot about that pathologist. >>> "Joe Nocito" 8/24/2006 7:42:04 PM >>> Lick the block!? Damn, I thought I was the only one doing that. I used to do that with Pap smears. "This one is negative, negative, negative, positive, negative, oooh, this has HPV". Y'all didn't think I'd let this go by without a comment, did you? I love Fridays!!! Ooops, it's only Thursday. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Rene J Buesa" To: "LeVier, Rebecca J" ; "Scott, Allison D" ; Sent: Thursday, August 24, 2006 3:21 PM Subject: RE: [Histonet] Wearing gloves while cutting sections > Rebecca: > I think that you should expalin your thoughtful and illustrious commettee > that once the tissue is fixed and processed (unless it is KJ disease case) > you can lick the block. > By the way, I once did just that in front of the safety officer of our > lab (she left me alone for ever. I still don't know if she left me alone > because she was convinced or because she thought that I was completely out > of my mind; it worked!). > Ren? J. > > "LeVier, Rebecca J" wrote: > I am not sure that the committee fully understood what the process was. > Could they have been looking at the wrong thing. Did they really mean to > site for this or were they just having a misunderstanding? If it were > me, I would try to get together with them to talk. For me, it is almost > impossible for me to grab a ribbon with gloves on. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, > Allison D > Sent: Thursday, August 24, 2006 3:07 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Wearing gloves while cutting sections > > While I was on vaction, the safety committee came around and did rounds > in the lab. They sited us for not wearing gloves while cutting > sections. My techs tried to explain to them that we did not have to > wear gloves, only when cutting frozen sections or while handling fresh > tissue. I have never worn gloves while cutting paraffin sections. Now > my manager wants me to write a policy in regards to not wearing gloves. > Does anyone have anything like this in place that they would be willing > to share with me. Your help would be greatly appreciated. > > Allison Scott HT(ASCP) > Histology Supervisor > LBJ Hospital > Houston, Texas 77026 > > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify the > sender by return e-mail and delete this e-mail and any attachments from > your computer system. > > To the extent the information in this e-mail and any attachments contain > protected health information as defined by the Health Insurance > Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR > Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is > confidential and/or privileged. This e-mail may also be confidential > and/or privileged under Texas law. The e-mail is for the use of only > the individual or entity named above. If you are not the intended > recipient, or any authorized representative of the intended recipient, > you are hereby notified that any review, dissemination or copying of > this e-mail and its attachments is strictly prohibited. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of intended recipient(s) and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by reply e-mail and > destroy all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Get your own web address for just $1.99/1st yr. We'll help. Yahoo! Small > Business. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.405 / Virus Database: 268.11.5/426 - Release Date: 8/23/2006 > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From rjbuesa <@t> yahoo.com Fri Aug 25 07:30:41 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 25 07:30:50 2006 Subject: [Histonet] Frozens section cutinng by resident Dr In-Reply-To: <013f01c6c7e5$6f396b30$131e80cb@p> Message-ID: <20060825123041.99437.qmail@web61216.mail.yahoo.com> Tahseen: That is standard practice in many a hospital. If you go to www.adasp.org/surveys/hot-line.htm there is a survey where many of the usual residents tasks and workloads are summarized. This is an interesting information. Ren? J. Tahseen wrote: Hi every body Are there any other hospitals out there where the pathologists or resident cut frozen section stain and mount their own cases? Muhammad Tahseen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. From tracy.bergeron <@t> crl.com Fri Aug 25 09:04:40 2006 From: tracy.bergeron <@t> crl.com (tracy.bergeron@crl.com) Date: Fri Aug 25 09:05:55 2006 Subject: [Histonet] Wearing gloves while cutting sections In-Reply-To: Message-ID: Hank solution would be HBSS Hanks Balanced Salts solution. It is used in cell culture, along with a lot of other similarly named solutions. I don't imagine it would taste all that great though, probably similar to drinking Epsom salts diluted in water. As of this year in our lab we are required to wear gloves while cutting. We have had a lot of changes in the area of safety here along with always wearing safety glasses, gloves while cutting, wearing goggles and impervious aprons while trimming in fixed tissue. All three were not required before the beginning of this year. Our company is trying to make sure that our labs all go above and beyond in the safety dept. Once I got used to the changes none of them were difficult to deal with. Cutting with gloves only took me a week or two to adjust to. Mainly because we use nitrile gloves. I am mildly allergic to latex so I avoid latex gloves like the plague, so that along with other reasons we use nitrile gloves in this lab for all procedures from cutting to special stains and trimming in of tissue. Nitrile gloves fit snugly so there is nothing loose to get in the way of the blade if you are picking up a ribbon with your fingers. Also someone mentioned forceps earlier, I use forceps and fingers to move a ribbon from the microtome to the water bath, mainly because it can be a tad windy in here and moving ribbons with two hands keeps things from flapping in the breeze so to speak. Tracy E. Bergeron, BS, HT, HTL (ASCP) Histotechnologist Charles River Laboratories Wilmington, MA 978-658-6000 x 1229 "Janice Mahoney" Sent by: histonet-bounces@lists.utsouthwestern.edu 08/25/2006 07:56 AM To , cc Subject Re: [Histonet] Wearing gloves while cutting sections I've been in this field for over 30 years and have never heard of Hank solution. Do I want to drink it? And Joe, if anyone out there in Histoland ever questioned whether or not you are a nut, they now know. >>> "Rebecca Barnhart" 08/25/2006 6:45 AM >>> Wow! I thought it was weird when I once witnessed a pathologist drink Hank solution to determine if it was similar to a drink. Of course the Hank was fresh. That experience explained a lot about that pathologist. >>> "Joe Nocito" 8/24/2006 7:42:04 PM >>> Lick the block!? Damn, I thought I was the only one doing that. I used to do that with Pap smears. "This one is negative, negative, negative, positive, negative, oooh, this has HPV". Y'all didn't think I'd let this go by without a comment, did you? I love Fridays!!! Ooops, it's only Thursday. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Rene J Buesa" To: "LeVier, Rebecca J" ; "Scott, Allison D" ; Sent: Thursday, August 24, 2006 3:21 PM Subject: RE: [Histonet] Wearing gloves while cutting sections > Rebecca: > I think that you should expalin your thoughtful and illustrious commettee > that once the tissue is fixed and processed (unless it is KJ disease case) > you can lick the block. > By the way, I once did just that in front of the safety officer of our > lab (she left me alone for ever. I still don't know if she left me alone > because she was convinced or because she thought that I was completely out > of my mind; it worked!). > Ren? J. > > "LeVier, Rebecca J" wrote: > I am not sure that the committee fully understood what the process was. > Could they have been looking at the wrong thing. Did they really mean to > site for this or were they just having a misunderstanding? If it were > me, I would try to get together with them to talk. For me, it is almost > impossible for me to grab a ribbon with gloves on. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, > Allison D > Sent: Thursday, August 24, 2006 3:07 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Wearing gloves while cutting sections > > While I was on vaction, the safety committee came around and did rounds > in the lab. They sited us for not wearing gloves while cutting > sections. My techs tried to explain to them that we did not have to > wear gloves, only when cutting frozen sections or while handling fresh > tissue. I have never worn gloves while cutting paraffin sections. Now > my manager wants me to write a policy in regards to not wearing gloves. > Does anyone have anything like this in place that they would be willing > to share with me. Your help would be greatly appreciated. > > Allison Scott HT(ASCP) > Histology Supervisor > LBJ Hospital > Houston, Texas 77026 > > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify the > sender by return e-mail and delete this e-mail and any attachments from > your computer system. > > To the extent the information in this e-mail and any attachments contain > protected health information as defined by the Health Insurance > Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR > Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is > confidential and/or privileged. This e-mail may also be confidential > and/or privileged under Texas law. The e-mail is for the use of only > the individual or entity named above. If you are not the intended > recipient, or any authorized representative of the intended recipient, > you are hereby notified that any review, dissemination or copying of > this e-mail and its attachments is strictly prohibited. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of intended recipient(s) and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by reply e-mail and > destroy all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Get your own web address for just $1.99/1st yr. We'll help. Yahoo! Small > Business. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.405 / Virus Database: 268.11.5/426 - Release Date: 8/23/2006 > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dorothy.L.Webb <@t> HealthPartners.Com Fri Aug 25 09:34:24 2006 From: Dorothy.L.Webb <@t> HealthPartners.Com (Webb, Dorothy L) Date: Fri Aug 25 09:34:36 2006 Subject: [Histonet] gloves Message-ID: <0E394B648E5284478A6CCB78E5AFDA270171FF6C@hpes1.HealthPartners.int> Rene...You make me laugh with your responses!! Wish I could work with you, as you seem to know something about everything and have the greatest comebacks in the world!! You could help all of us with those in the management world that do not understand histology, but always try to impose these "rules" that do not apply to our areas!! Thanks for making my day!! ________________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this e-mail in error, please immediately notify the HealthPartners Support Center by telephone at (952) 967-6600. You will be reimbursed for reasonable costs incurred in notifying us. From histo20 <@t> hotmail.com Fri Aug 25 09:52:29 2006 From: histo20 <@t> hotmail.com (Paula Wilder) Date: Fri Aug 25 09:52:34 2006 Subject: [Histonet] Immuno - Her2Neu Message-ID: Has anyone found a commercial control for Her2/Neu that includes 1+, 2+, and 3+ tissue all on one slide? Dako will not sell theirs separately, and CellMarque has only 3+ on their control slides. Thanks so much! Paula Wilder St. Joseph Medical Center From tissuearray <@t> hotmail.com Fri Aug 25 10:06:10 2006 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Fri Aug 25 10:06:16 2006 Subject: [Histonet] TMA Book In-Reply-To: <127D612DBCD77546A12C4746F7491C7F111AD1@lewis.afip.osd.mil> Message-ID: If you go to my website on creating tissuearrays I have several resources on the subject. Articles and videos. www.arrayworkshop.com And I do answer question through email or phone. I have given workshops at two NSH meetings on tissue microarrays. Thom Jensen >From: "Achstetter, Virginia A." >To: >CC: histonet@lists.utsouthwestern.edu >Subject: [Histonet] TMA Book >Date: Fri, 25 Aug 2006 06:50:32 -0400 >MIME-Version: 1.0 >Received: from swlx162.swmed.edu ([199.165.152.162]) by >bay0-mc7-f18.bay0.hotmail.com with Microsoft SMTPSVC(6.0.3790.2444); Fri, >25 Aug 2006 03:51:05 -0700 >Received: from localhost ([127.0.0.1] helo=swlx162.swmed.edu)by >swlx162.swmed.edu with esmtp (Exim 4.34)id 1GGZH8-0007ND-JR; Fri, 25 Aug >2006 05:50:58 -0500 >Received: from [199.165.152.166] (helo=swlx166.swmed.edu)by >swlx162.swmed.edu with esmtp (Exim 4.34) id 1GGZH4-0007Mh-M4for >histonet@lists.utsouthwestern.edu; Fri, 25 Aug 2006 05:50:54 -0500 >Received: from pathology.swmed.edu ([129.112.48.201])by swlx166.swmed.edu >with esmtp (Exim 4.44) id 1GGZH3-0001ZQ-Eufor >histonet@lists.utsouthwestern.edu; Fri, 25 Aug 2006 05:50:54 -0500 >Received: from swlx166.swmed.edu (199.165.152.166) by >pathology.swmed.eduwith ESMTP (Eudora Internet Mail Server 3.1.5) for >; Fri, 25 Aug 2006 05:30:03 -0500 >Received: from lewisjr.afip.osd.mil ([198.97.78.51])by swlx166.swmed.edu >with esmtp (Exim 4.44) id 1GGZH2-0001Z4-F1for histonet@pathology.swmed.edu; >Fri, 25 Aug 2006 05:50:53 -0500 >Received: from lewis.afip.osd.mil ([10.90.0.50]) by lewisjr.afip.osd.mil >withMicrosoft SMTPSVC(6.0.3790.1830); Fri, 25 Aug 2006 06:50:32 -0400 >X-Message-Info: LsUYwwHHNt1vKQpZNB84SXxJqwy79yS2ZFneudwyjvA= >X-MimeOLE: Produced By Microsoft Exchange V6.5 >Content-class: urn:content-classes:message >X-MS-Has-Attach: X-MS-TNEF-Correlator: Thread-Topic: TMA Book >Thread-Index: AcbINEnXeEpWcwXvSt6AVgxmtG7HjQ== >X-OriginalArrivalTime: 25 Aug 2006 10:50:32.0609 >(UTC)FILETIME=[49A5B910:01C6C834] >X-Scan-Signature: 18b8f45f5f122038853a7119e027f2bd >X-Content-Filtered-By: Mailman/MimeDel 2.1.5 >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.1.5 >Precedence: list >List-Id: For the exchange of information pertaining to histotechnology >andrelated fields >List-Unsubscribe: >, > >List-Archive: >List-Post: >List-Help: >List-Subscribe: >, >Errors-To: histonet-bounces@lists.utsouthwestern.edu >X-Scan-Signature: 2ab8d6fe94b74561233a4cdd2e311ef7 >X-SA-Exim-Connect-IP: 127.0.0.1 >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >X-Spam-Checker-Version: SpamAssassin 2.64 (2004-01-11) on swlx162.swmed.edu >X-Spam-Level: X-Spam-Status: No, hits=0.0 required=5.5 tests=none >autolearn=no version=2.64 >X-SA-Exim-Version: 4.0 (built Wed, 07 Apr 2004 12:14:41 -0500) >X-SA-Exim-Scanned: Yes (on swlx162.swmed.edu) >Return-Path: histonet-bounces@lists.utsouthwestern.edu > >Are there any good books out on how to prepare a tma block, trouble >shooting, cutting techniques etc. > >Ginny Achstetter >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Annette_hall <@t> pa-ucl.com Fri Aug 25 10:08:42 2006 From: Annette_hall <@t> pa-ucl.com (Annette Hall) Date: Fri Aug 25 10:08:53 2006 Subject: [Histonet] Immuno - Her2Neu Message-ID: <9FC023A4AB52BB4D87DC6456081A822C070BDE@mercury.pa-ucl.com> We resorted to making our own Her2 control with multiple degrees of staining. It can be a pain to develop a batch, but once we have identified the tissues, we make up 4-5 blocks that last us awhile. Annette J Hall, MT Micro/Cyto/Histo Supervisor United Clinical Labs Dubuque, IA 52001 -----Original Message----- From: Paula Wilder [mailto:histo20@hotmail.com] Sent: Friday, August 25, 2006 9:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Immuno - Her2Neu Has anyone found a commercial control for Her2/Neu that includes 1+, 2+, and 3+ tissue all on one slide? Dako will not sell theirs separately, and CellMarque has only 3+ on their control slides. Thanks so much! Paula Wilder St. Joseph Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From steve.penney <@t> hotmail.com Fri Aug 25 10:19:57 2006 From: steve.penney <@t> hotmail.com (Steve Penney) Date: Fri Aug 25 10:20:07 2006 Subject: [Histonet] Do you know a good supplier for biopsy cassettes ? Message-ID: Good morning all histonetters ! I’d like to know where you buy your cassettes. I’m a student in Canada (French Canadian, so I’m sorry if my E-mail is not clear) and I’m looking for a biopsy cassette with 6 compartments. I found the product on Fisher Canada website and it’s almost 700 $ dollars (US of course!). I have a limited budget! Is that the normal price or you know other supplier who distribute in Canada with better price. The item number I found is 15-182-706A on Fisher website. Of course an equivalent product is fine with me. Thank you and have a great week-end Steve _________________________________________________________________ Play Q6 for your chance to WIN great prizes. http://q6trivia.imagine-live.com/enca/landing From Rcartun <@t> harthosp.org Fri Aug 25 10:19:54 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Aug 25 10:20:56 2006 Subject: [Histonet] Frozens section cutinng by resident Dr In-Reply-To: <013f01c6c7e5$6f396b30$131e80cb@p> References: <013f01c6c7e5$6f396b30$131e80cb@p> Message-ID: <44EEDCDA0200007700001964@hcnwgwds01.hh.chs> Yes, we do it all the time. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Tahseen" 08/24/06 9:26 PM >>> Hi every body Are there any other hospitals out there where the pathologists or resident cut frozen section stain and mount their own cases? Muhammad Tahseen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Rcartun <@t> harthosp.org Fri Aug 25 10:23:08 2006 From: Rcartun <@t> harthosp.org (Richard Cartun) Date: Fri Aug 25 10:23:56 2006 Subject: [Histonet] Immuno - Her2Neu In-Reply-To: References: Message-ID: <44EEDD9C0200007700001968@hcnwgwds01.hh.chs> Why don't you make your own? Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Paula Wilder" 08/25/06 10:52 AM >>> Has anyone found a commercial control for Her2/Neu that includes 1+, 2+, and 3+ tissue all on one slide? Dako will not sell theirs separately, and CellMarque has only 3+ on their control slides. Thanks so much! Paula Wilder St. Joseph Medical Center _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cpomajzl <@t> cpllabs.com Fri Aug 25 11:04:47 2006 From: cpomajzl <@t> cpllabs.com (Chris Pomajzl) Date: Fri Aug 25 10:58:04 2006 Subject: [Histonet] Do you know a good supplier for biopsy cassettes ? References: Message-ID: <006301c6c860$3027d410$26fca8c0@CSP> MERDE! C'est tres expensive. ----- Original Message ----- From: "Steve Penney" To: Sent: Friday, August 25, 2006 10:19 AM Subject: [Histonet] Do you know a good supplier for biopsy cassettes ? > Good morning all histonetters ! > > I'd like to know where you buy your cassettes. I'm a student in Canada > (French Canadian, so I'm sorry if my E-mail is not clear) and I'm looking > for a biopsy cassette with 6 compartments. I found the product on Fisher > Canada website and it's almost 700 $ dollars (US of course!). I have a > limited budget! Is that the normal price or you know other supplier who > distribute in Canada with better price. The item number I found is > 15-182-706A on Fisher website. Of course an equivalent product is fine with > me. > > Thank you and have a great week-end > > Steve > > _________________________________________________________________ > Play Q6 for your chance to WIN great prizes. > http://q6trivia.imagine-live.com/enca/landing > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tissuearray <@t> hotmail.com Fri Aug 25 10:58:47 2006 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Fri Aug 25 10:59:01 2006 Subject: [Histonet] TMA Book Message-ID: Virginia The Beecher I believe is the best instrument on the market. If you go to my website I did a review on the Chemicon TMArrrayer. It has problems that make the array construction process complicated and longer. If you can use the beecher I would recommend it. I tested the Chemicon last year at my lab so I have used it and tried to work out the problems with the company. But Chemicon was not corporative. I have articles on how to construct TMAs that are very helpful to now techs. The first page on my website, at the bottom, tell were the articles are. They are in the Journal of Histology. If you have questions please feel free to contact me any time. The tape method is good for frozens but the tape destroys paraffin samples. They are compressed and most times unreadable. Tech that use the tape usually have a hard time cutting in the first place. A good tech should be able to cut without much problem. I have a section on tape method problems on my website: http://arrayworkshop.com/Notes_2.html If you need help cutting I can explain some techniques I use to achieve nice sections with TMA blocks. I have tried to work with the Tape company, Instrumedics, but they were not very helpful and they couldn't solve the poor sectioning problems. Good luck, Thom >From: "Achstetter, Virginia A." >To: "Thom Jensen" >Subject: RE: [Histonet] TMA Book >Date: Fri, 25 Aug 2006 11:22:20 -0400 >MIME-Version: 1.0 >Received: from redstoned.afip.osd.mil ([198.97.78.48]) by >bay0-mc11-f18.bay0.hotmail.com with Microsoft SMTPSVC(6.0.3790.2444); Fri, >25 Aug 2006 08:22:37 -0700 >Received: from lewis.afip.osd.mil ([10.90.0.50]) by redstoned.afip.osd.mil >with Microsoft SMTPSVC(6.0.3790.1830); Fri, 25 Aug 2006 11:22:20 -0400 >X-Message-Info: LsUYwwHHNt1JCgzkhwVUxrinDw2ASROAvjJm6+WVwzM= >X-MimeOLE: Produced By Microsoft Exchange V6.5 >Content-class: urn:content-classes:message >Disposition-Notification-To: "Achstetter, Virginia A." > >X-MS-Has-Attach: X-MS-TNEF-Correlator: Thread-Topic: [Histonet] TMA Book >Thread-Index: AcbIV/j3ZE5Yt73RRVGUn/5aGG8/HAAAgA9Q >Return-Path: Virginia.Achstetter@afip.osd.mil >X-OriginalArrivalTime: 25 Aug 2006 15:22:20.0688 (UTC) >FILETIME=[42051900:01C6C85A] > >You are the best resource so far. I believe the microarryer that I >will be using is a chemicon. My present department has the beecher >manual arrayer but we never used it. You have DVD's available. Do you >have a book with techniques? Also, you prefer to cut the block just >like a paraffin block. So many people use the tape method. Why do >they prefer tape? > >-----Original Message----- >From: Thom Jensen [mailto:tissuearray@hotmail.com] >Sent: Friday, August 25, 2006 11:06 AM >To: Achstetter, Virginia A.; histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] TMA Book > >If you go to my website on creating tissuearrays I have several >resources on >the subject. Articles and videos. www.arrayworkshop.com > >And I do answer question through email or phone. I have given workshops >at two NSH meetings on tissue microarrays. > >Thom Jensen > > > >From: "Achstetter, Virginia A." > >To: > >CC: histonet@lists.utsouthwestern.edu > >Subject: [Histonet] TMA Book > >Date: Fri, 25 Aug 2006 06:50:32 -0400 > >MIME-Version: 1.0 > >Received: from swlx162.swmed.edu ([199.165.152.162]) by > >bay0-mc7-f18.bay0.hotmail.com with Microsoft SMTPSVC(6.0.3790.2444); > >Fri, > >25 Aug 2006 03:51:05 -0700 > >Received: from localhost ([127.0.0.1] helo=swlx162.swmed.edu)by > >swlx162.swmed.edu with esmtp (Exim 4.34)id 1GGZH8-0007ND-JR; Fri, 25 > >Aug > >2006 05:50:58 -0500 > >Received: from [199.165.152.166] (helo=swlx166.swmed.edu)by > >swlx162.swmed.edu with esmtp (Exim 4.34) id 1GGZH4-0007Mh-M4for > >histonet@lists.utsouthwestern.edu; Fri, 25 Aug 2006 05:50:54 -0500 > >Received: from pathology.swmed.edu ([129.112.48.201])by > >swlx166.swmed.edu with esmtp (Exim 4.44) id 1GGZH3-0001ZQ-Eufor > >histonet@lists.utsouthwestern.edu; Fri, 25 Aug 2006 05:50:54 -0500 > >Received: from swlx166.swmed.edu (199.165.152.166) by > >pathology.swmed.eduwith ESMTP (Eudora Internet Mail Server 3.1.5) for > >; Fri, 25 Aug 2006 05:30:03 -0500 > >Received: from lewisjr.afip.osd.mil ([198.97.78.51])by > >swlx166.swmed.edu with esmtp (Exim 4.44) id 1GGZH2-0001Z4-F1for > >histonet@pathology.swmed.edu; Fri, 25 Aug 2006 05:50:53 -0500 > >Received: from lewis.afip.osd.mil ([10.90.0.50]) by > >lewisjr.afip.osd.mil withMicrosoft SMTPSVC(6.0.3790.1830); Fri, 25 Aug > >2006 06:50:32 -0400 > >X-Message-Info: LsUYwwHHNt1vKQpZNB84SXxJqwy79yS2ZFneudwyjvA= > >X-MimeOLE: Produced By Microsoft Exchange V6.5 > >Content-class: urn:content-classes:message > >X-MS-Has-Attach: X-MS-TNEF-Correlator: Thread-Topic: TMA Book > >Thread-Index: AcbINEnXeEpWcwXvSt6AVgxmtG7HjQ== > >X-OriginalArrivalTime: 25 Aug 2006 10:50:32.0609 > >(UTC)FILETIME=[49A5B910:01C6C834] > >X-Scan-Signature: 18b8f45f5f122038853a7119e027f2bd > >X-Content-Filtered-By: Mailman/MimeDel 2.1.5 > >X-BeenThere: histonet@lists.utsouthwestern.edu > >X-Mailman-Version: 2.1.5 > >Precedence: list > >List-Id: For the exchange of information pertaining to histotechnology > >andrelated fields > >List-Unsubscribe: > >, > > > >List-Archive: > >List-Post: > >List-Help: > > > >List-Subscribe: > >, >tonet-request@lists.utsouthwestern.edu?subject=subscribe> > >Errors-To: histonet-bounces@lists.utsouthwestern.edu > >X-Scan-Signature: 2ab8d6fe94b74561233a4cdd2e311ef7 > >X-SA-Exim-Connect-IP: 127.0.0.1 > >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu > >X-Spam-Checker-Version: SpamAssassin 2.64 (2004-01-11) on > >swlx162.swmed.edu > >X-Spam-Level: X-Spam-Status: No, hits=0.0 required=5.5 tests=none > >autolearn=no version=2.64 > >X-SA-Exim-Version: 4.0 (built Wed, 07 Apr 2004 12:14:41 -0500) > >X-SA-Exim-Scanned: Yes (on swlx162.swmed.edu) > >Return-Path: histonet-bounces@lists.utsouthwestern.edu > > > >Are there any good books out on how to prepare a tma block, trouble > >shooting, cutting techniques etc. > > > >Ginny Achstetter > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > From tp2 <@t> medicine.wisc.edu Fri Aug 25 11:09:56 2006 From: tp2 <@t> medicine.wisc.edu (Thomas Pier) Date: Fri Aug 25 11:10:24 2006 Subject: [Histonet] CD123 and TMAs Message-ID: <44EEDA84020000DF00001071@gwmail.medicine.wisc.edu> Hi everybody, I'm a little confused by my current situation. I had worked up CD123 (from BD) in a tonsil control and it looked great. When I run the same condition on a lymphoma TMA the possitive control looks good and the TMA does not. Has anybody out there had a similar problem or have any idea of what may be cuasing this? Any help would be greatly appreciated. Tom Pier From PMonfils <@t> Lifespan.org Fri Aug 25 11:33:59 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Fri Aug 25 11:34:10 2006 Subject: [Histonet] Do you know a good supplier for biopsy cassettes ? Message-ID: <09C945920A6B654199F7A58A1D7D1FDE0171778E@lsexch.lsmaster.lifespan.org> Try this: http://www.emsdiasum.com/microscopy/products/histology/cassettes.aspx From ploykasek <@t> phenopath.com Fri Aug 25 12:00:30 2006 From: ploykasek <@t> phenopath.com (Patti Loykasek) Date: Fri Aug 25 12:01:39 2006 Subject: [Histonet] CD123 and TMAs In-Reply-To: <44EEDA84020000DF00001071@gwmail.medicine.wisc.edu> Message-ID: Hi Tom, Exactly how does the TMA look? Is it staining at all? Is it overstained? Over-retreived? With a little more information, we can give better answers. Patti Loykasek BS, HTL, QIHC PhenoPath Laboratories Seattle, WA > Hi everybody, > I'm a little confused by my current situation. I had worked up CD123 > (from BD) in a tonsil control and it looked great. When I run the same > condition on a lymphoma TMA the possitive control looks good and the TMA > does not. Has anybody out there had a similar problem or have any idea > of what may be cuasing this? Any help would be greatly appreciated. > > Tom Pier > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------- This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. From tissuearray <@t> hotmail.com Fri Aug 25 12:03:41 2006 From: tissuearray <@t> hotmail.com (Thom Jensen) Date: Fri Aug 25 12:03:52 2006 Subject: [Histonet] CD123 and TMAs In-Reply-To: <44EEDA84020000DF00001071@gwmail.medicine.wisc.edu> Message-ID: Are you doing stains on a tma that was cut using the tape method? Thom >From: "Thomas Pier" >To: >Subject: [Histonet] CD123 and TMAs >Date: Fri, 25 Aug 2006 11:09:56 -0500 >MIME-Version: 1.0 >Received: from swlx162.swmed.edu ([199.165.152.162]) by >bay0-mc6-f7.bay0.hotmail.com with Microsoft SMTPSVC(6.0.3790.2444); Fri, 25 >Aug 2006 09:10:42 -0700 >Received: from localhost ([127.0.0.1] helo=swlx162.swmed.edu)by >swlx162.swmed.edu with esmtp (Exim 4.34)id 1GGeGH-0002o8-RD; Fri, 25 Aug >2006 11:10:26 -0500 >Received: from [199.165.152.167] (helo=swlx167.swmed.edu)by >swlx162.swmed.edu with esmtp (Exim 4.34) id 1GGeGE-0002ny-5Nfor >histonet@lists.utsouthwestern.edu; Fri, 25 Aug 2006 11:10:23 -0500 >Received: from lists.medicine.wisc.edu >([128.104.208.5]helo=mailout.medicine.wisc.edu)by swlx167.swmed.edu with >esmtp (Exim 4.44) id 1GGeGA-0006tF-LFfor histonet@lists.utsouthwestern.edu; >Fri, 25 Aug 2006 11:10:22 -0500 >Received: from gwmail.medicine.wisc.edu >(gwmail.medicine.wisc.edu[128.104.208.34])by mailout.medicine.wisc.edu >(8.13.1/8.13.1) with ESMTP idk7PGA82s006364for >; Fri, 25 Aug 2006 11:10:13 -0500 >Received: from Medicine1-MTA by gwmail.medicine.wisc.eduwith >Novell_GroupWise; Fri, 25 Aug 2006 11:10:06 -0500 >X-Message-Info: LsUYwwHHNt26AsaAA0NCCboKDwfuXN5jlUkr4fQxcAc= >X-Mailer: Novell GroupWise Internet Agent 7.0.1 >X-UWMedicine-MailScanner-Information: Please contact the ISP for >moreinformation >X-UWMedicine-MailScanner: Found to be clean >X-UWMedicine-MailScanner-From: tp2@medicine.wisc.edu >X-Scan-Signature: 2ab8d6fe94b74561233a4cdd2e311ef7 >X-BeenThere: histonet@lists.utsouthwestern.edu >X-Mailman-Version: 2.1.5 >Precedence: list >List-Id: For the exchange of information pertaining to histotechnology >andrelated fields >List-Unsubscribe: >, > >List-Archive: >List-Post: >List-Help: >List-Subscribe: >, >Errors-To: histonet-bounces@lists.utsouthwestern.edu >X-Scan-Signature: 801ae9c9b293b3f34c4a36cf4e57e505 >X-SA-Exim-Connect-IP: 127.0.0.1 >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu >X-Spam-Checker-Version: SpamAssassin 2.64 (2004-01-11) on swlx162.swmed.edu >X-Spam-Level: X-Spam-Status: No, hits=0.0 required=5.5 tests=none >autolearn=no version=2.64 >X-SA-Exim-Version: 4.0 (built Wed, 07 Apr 2004 12:14:41 -0500) >X-SA-Exim-Scanned: Yes (on swlx162.swmed.edu) >Return-Path: histonet-bounces@lists.utsouthwestern.edu >X-OriginalArrivalTime: 25 Aug 2006 16:10:42.0247 (UTC) >FILETIME=[037BDD70:01C6C861] > >Hi everybody, >I'm a little confused by my current situation. I had worked up CD123 >(from BD) in a tonsil control and it looked great. When I run the same >condition on a lymphoma TMA the possitive control looks good and the TMA >does not. Has anybody out there had a similar problem or have any idea >of what may be cuasing this? Any help would be greatly appreciated. > >Tom Pier > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Dolores_Fischer <@t> baxter.com Fri Aug 25 12:25:37 2006 From: Dolores_Fischer <@t> baxter.com (Dolores_Fischer@baxter.com) Date: Fri Aug 25 12:25:45 2006 Subject: [Histonet] lectins Message-ID: Hello everyone in histoland! I finally need to utilze the expertise of all of you fellow histonetters. I have limited experience in immunohistochemistry (give me a protocol and show me the reagents and I can follow). I am now in a position where I will have to work out protocols on my own (oh no! I will have to think!) Here is the first assignment. I need to work up a protocol using isolectin B4 as a marker for endothelial cells in rat tissue. Would one follow the application of the isolectin with an ABC reagent or, as someone suggested to me, use a streptavidin derivitive? Does one need to begin with an antigen retreivel? I was told that working with lectins can be tricky and are considered by some to be "old school" methods. Are there better, newer markers or methods for endothelial cells in rat tissue? I also have a reference paper that states that 100% methanol was used as a fixative. Why use 100% methanol? Is 24 hr fixation in NBF acceptable (or preferred)? Any tips, comments, opinions, protocol sharing to lead me down the successful lectin path would be appreciated. Thanks and Happy Friday to all, not much venting today............................well .............. those people who walk in from other labs and use your "stuff" and reagents 'cause they ain't got any can be kinda irritating...........................time for a training lesson...........................stay out of my kitchen and return my "stuff"! Dolores Fischer 847-270-5509 The information transmitted is intended only for the person(s)or entity to which it is addressed and may contain confidential and/or legally privileged material. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive privilege or confidentiality. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by entities other than the intended recipient is prohibited. If you receive this in error, please contact the sender and delete the material from any computer. For Translation: http://www.baxter.com/email_disclaimer From antyler <@t> uncg.edu Fri Aug 25 12:24:16 2006 From: antyler <@t> uncg.edu (Amber Tyler ANTYLER) Date: Fri Aug 25 12:36:45 2006 Subject: [Histonet] products suggestions for snap freezing Message-ID: I am trying to find the safest way to do snap freezing with mouse brains. I am looking for the right materials to keep isopentane (stainless steel beaker?) over dry ice (polyvinal chloride lab pan). I would prefer to keep the beaker stable somehow. I don't want to risk it tipping over. Are there standard products for doing snap freezing? Thank you in advance. Amber Amber N. Tyler, Ph.D. Research Scientist Dept of Psychology (PO Box 26170) 296 Eberhart Building, Walker Avenue University of North Carolina - Greensboro Greensboro, NC 27402 antyler@uncg.edu From Xilong.Li <@t> UTSouthwestern.edu Fri Aug 25 12:39:32 2006 From: Xilong.Li <@t> UTSouthwestern.edu (Xilong Li) Date: Fri Aug 25 12:39:53 2006 Subject: [Histonet] question about sirius red stain Message-ID: <44EEEF84020000F0000063FB@swnw124.swmed.edu> Hi, All, I stain collagen of frozen muscle section using regular sirius red staining protocol(0.5g sirius red F3B in 500ml saturated picric acid, section were stained 1h in solution, then wash in 0.5% acidified water shortly). The background of images was all red under ordinary microscopy, but not yellow as expected, collagen can be seen barely with dark red. I extended washing time in acidified water, but no improvement was gotten. I just wonder any one had similar experience and how to improve it? Thanks in advance. Dr. Xilong Li Hypertension Division, Internal Medicine University of Texas Southwestern Medical Center 5323 Harry Hiness Blvd-J4.142 Dallas, TX 75390 Tel: 214-648-9966(L) Fax: 214-648-7902 From ROrr <@t> enh.org Fri Aug 25 12:42:14 2006 From: ROrr <@t> enh.org (Orr, Rebecca) Date: Fri Aug 25 12:42:22 2006 Subject: [Histonet] Acetylcholinesterase/Hazel Horn Message-ID: Hi Everyone, I saw an entry in the archives that Hazel has a Acetylcholinasesterase procedure. Does anyone have this procedure or connect me with Hazel? Have a great weekend, Becky Becky Orr, CLA, HT(ASCP) QIHC Assistant Manager, Anatomic Pathology Evanston Northwestern Healthcare 847-570-2763 -----Original Message----- From gcallis <@t> montana.edu Fri Aug 25 13:03:28 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Fri Aug 25 13:03:38 2006 Subject: [Histonet] lectins In-Reply-To: References: Message-ID: <6.0.0.22.1.20060825113558.01b5b978@gemini.msu.montana.edu> What you want to do is NOT immunostaining but binding the Isolectin-BF4 to the sugar residues in the rat endothelial cells. For a negative control you need to use the inhibiting sugar Inhibiting Sugar: 500 mM galactose or 100 mM raffinose and incubate the working concentration of isolectin-B4 in this at RT for 30 min to one hour, even overnight, and apply to the tissue section. PBS is NOT a negative control for lectins. Isolectin-B4 if NOT an antibody so this is NOT immunostaining. Be sure to use TBS and not PBS as the buffer and no serums in the reagents since lectins can bind to sugar residues in serums. We simply use TBS with 0.05% Tween 20, and for fluorescence, reduce the Tween to 0.025% just so it acts as a sheeting agent for good flow over the tissue. BSA is ok to use. Some lectins require lectin buffer that is basically TBS with Mg and Ca added. This should be posted in Histonet Archives. With fresh snap frozen, in our case, murine tissue, we use 75%acetone/25% absolute ethanol for 5 min at RT, and go directly to buffer, no more drying after fixation. I am not sure what methanol will do as a fixative for lectins, but one can also do formalin fixed paraffin tissue, followed by trypsin digestion, use isolectin B4- biotinylated and come back with either Strepavidin-enzyme, then chromogen OR Strepavidin-Alexa fluor for flourescence work. For frozen sections, lectin incubation is adequate at 30 min, 1 hr for FFPE tissue sections. Many lectins are conjugated to fluorescein or TRITC (Vector has your lectin fluoresceinated, and if you do fresh frozen sections, fixed with acetone, or acetone alcohol, the isolectin B4-fluoresceine conjugate should work well at around a 1:250 dilution of the 0.5mg concentration. Do NOT use ABC with this method, no secondaries are needed unless you use the isolectin B4 unconjugated, then you can detect with an antibody that is anti-isolectin B4 (if you can find this??) Try either the direct FITC conjugate or biotinylated conjugate first. The FITC may conflict with any autofluorescence induced by formalin fixation, but one could use the biotin and come back with either chromogen method or red fluorophore conjugated to Strepavdin (Molecular Probes, SA 546, SA-488 is the FITC equivalent). For biotinylated lectin, we buy our Strepavidin HRP or AP from Biosource, Southern Biotechnology has good SA conjugates as does Jackson Immunoresearch. Be aware the lectins can bind to other sites in tissues, if the sugar residue it recognizes is elsewhere, and there is nothing that blocks this binding. We have had to do two lectins so see colocalization in order to sort out what is separate staining elsewhere for a single lectin - There is a whole book on lectins that is fantastic. Lectin Histochemistry, a concise practical handbook, Brooks SA and Leathem AJC. ISBN # 1859961002. Go to Journal of Histochemistry Cytochemistry and pick up the publications on lectins, one is like a review and tells what lectins bind where, superb! For help on how to work with lectins, ask Vectors tech rep Craig Pow (cspow@vectorlabs.com) - he helped me understand a great deal about lectins and explains why you use inhibiting sugar as the negative control plus buffer issues, what to avoid. Nice fellow to visit with. If you want to do immunostaining for rat endothelial cells, you can always search BD Pharmingen or SEROTEC websites for a monoclonal antibody and do either true IHC or IFA work. At 11:25 AM 8/25/2006, you wrote: >Hello everyone in histoland! > >I finally need to utilze the expertise of all of you fellow histonetters. >I have limited experience in immunohistochemistry (give me a protocol and >show me the reagents and I can follow). I am now in a position where I >will have to work out protocols on my own (oh no! I will have to think!) >Here is the first assignment. I need to work up a protocol using >isolectin B4 as a marker for endothelial cells in rat tissue. Would one >follow the application of the isolectin with an ABC reagent or, as someone >suggested to me, use a streptavidin derivitive? Does one need to begin >with an antigen retreivel? I was told that working with lectins can be >tricky and are considered by some to be "old school" methods. Are there >better, newer markers or methods for endothelial cells in rat tissue? I >also have a reference paper that states that 100% methanol was used as a >fixative. Why use 100% methanol? Is 24 hr fixation in NBF acceptable >(or preferred)? Any tips, comments, opinions, protocol sharing to lead >me down the successful lectin path would be appreciated. Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From langxingpan <@t> pantomics.com Fri Aug 25 13:14:28 2006 From: langxingpan <@t> pantomics.com (Langxing Pan) Date: Fri Aug 25 13:14:39 2006 Subject: [Histonet] RE: IHC control for Her2/Neu Message-ID: <000801c6c872$4ebddb10$220110ac@Familyroom> Dear Paula, We provide several types of IHC control tissue arrays including HER2/Neu 4.5mm four cores (+++, ++, +, -) tissue array which has been validated by UK NEQAS IHC center. We will soon provide these products (either in section or block forms) to routine IHC labs with special prices. I am happy to send you more information about these products. Langxing Pan, M.D., Ph.D. Pantomics, Inc. 457 Mariposa Street San Francisco, CA 94107, USA Tel: 1-415-863-2380 Fax: 1-510-653-1227 langxingpan@pantomics.com www.pantomics.com Message: 15 Date: Fri, 25 Aug 2006 14:52:29 +0000 From: "Paula Wilder" Subject: [Histonet] Immuno - Her2Neu To: histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; format=flowed Has anyone found a commercial control for Her2/Neu that includes 1+, 2+, and 3+ tissue all on one slide? Dako will not sell theirs separately, and CellMarque has only 3+ on their control slides. Thanks so much! Paula Wilder St. Joseph Medical Center From abright <@t> brightinstruments.com Fri Aug 25 14:53:37 2006 From: abright <@t> brightinstruments.com (Alan Bright) Date: Fri Aug 25 14:41:21 2006 Subject: [Histonet] products suggestions for snap freezing Message-ID: Dear Amber, Our Clini-RF would suit your requirements with no need for dry ice.. Regards Alan Bright Bright Instrument Company Huntingdon England www.brightinstruments.com -----Original Message----- From: Amber Tyler ANTYLER [mailto:antyler@uncg.edu] Sent: Fri 25/08/2006 18:24 To: histonet@lists.utsouthwestern.edu Cc: Subject: [Histonet] products suggestions for snap freezing I am trying to find the safest way to do snap freezing with mouse brains. I am looking for the right materials to keep isopentane (stainless steel beaker?) over dry ice (polyvinal chloride lab pan). I would prefer to keep the beaker stable somehow. I don't want to risk it tipping over. Are there standard products for doing snap freezing? Thank you in advance. Amber Amber N. Tyler, Ph.D. Research Scientist Dept of Psychology (PO Box 26170) 296 Eberhart Building, Walker Avenue University of North Carolina - Greensboro Greensboro, NC 27402 antyler@uncg.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From cwscouten <@t> myneurolab.com Fri Aug 25 14:59:09 2006 From: cwscouten <@t> myneurolab.com (Charles Scouten) Date: Fri Aug 25 14:58:55 2006 Subject: [Histonet] TMA Book Message-ID: <5784D843593D874C93E9BADCB87342AB01307532@tpiserver03.Coretech-holdings.com> Instrumedics is now a part of the Vibratome company. We would be pleased to help any TMA lab with tape transfer. There are separate kits for frozen or paraffin sections, and many labs around the world are finding tape transfer valuable for paraffin or frozen TMA. Cordially, Charles W. Scouten, Ph.D. myNeuroLab.com 5918 Evergreen Blvd. St. Louis, MO 63134 Ph: 314 522 0300 x 342 FAX 314 522 0377 cwscouten@myneurolab.com http://www.myneurolab.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thom Jensen Sent: Friday, August 25, 2006 10:59 AM To: Virginia.Achstetter@afip.osd.mil; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] TMA Book Virginia The Beecher I believe is the best instrument on the market. If you go to my website I did a review on the Chemicon TMArrrayer. It has problems that make the array construction process complicated and longer. If you can use the beecher I would recommend it. I tested the Chemicon last year at my lab so I have used it and tried to work out the problems with the company. But Chemicon was not corporative. I have articles on how to construct TMAs that are very helpful to now techs. The first page on my website, at the bottom, tell were the articles are. They are in the Journal of Histology. If you have questions please feel free to contact me any time. The tape method is good for frozens but the tape destroys paraffin samples. They are compressed and most times unreadable. Tech that use the tape usually have a hard time cutting in the first place. A good tech should be able to cut without much problem. I have a section on tape method problems on my website: http://arrayworkshop.com/Notes_2.html If you need help cutting I can explain some techniques I use to achieve nice sections with TMA blocks. I have tried to work with the Tape company, Instrumedics, but they were not very helpful and they couldn't solve the poor sectioning problems. Good luck, Thom >From: "Achstetter, Virginia A." >To: "Thom Jensen" >Subject: RE: [Histonet] TMA Book >Date: Fri, 25 Aug 2006 11:22:20 -0400 >MIME-Version: 1.0 >Received: from redstoned.afip.osd.mil ([198.97.78.48]) by >bay0-mc11-f18.bay0.hotmail.com with Microsoft SMTPSVC(6.0.3790.2444); >Fri, >25 Aug 2006 08:22:37 -0700 >Received: from lewis.afip.osd.mil ([10.90.0.50]) by >redstoned.afip.osd.mil with Microsoft SMTPSVC(6.0.3790.1830); Fri, 25 >Aug 2006 11:22:20 -0400 >X-Message-Info: LsUYwwHHNt1JCgzkhwVUxrinDw2ASROAvjJm6+WVwzM= >X-MimeOLE: Produced By Microsoft Exchange V6.5 >Content-class: urn:content-classes:message >Disposition-Notification-To: "Achstetter, Virginia A." > >X-MS-Has-Attach: X-MS-TNEF-Correlator: Thread-Topic: [Histonet] TMA >Book >Thread-Index: AcbIV/j3ZE5Yt73RRVGUn/5aGG8/HAAAgA9Q >Return-Path: Virginia.Achstetter@afip.osd.mil >X-OriginalArrivalTime: 25 Aug 2006 15:22:20.0688 (UTC) >FILETIME=[42051900:01C6C85A] > >You are the best resource so far. I believe the microarryer that I >will be using is a chemicon. My present department has the beecher >manual arrayer but we never used it. You have DVD's available. Do you >have a book with techniques? Also, you prefer to cut the block just >like a paraffin block. So many people use the tape method. Why do >they prefer tape? > >-----Original Message----- >From: Thom Jensen [mailto:tissuearray@hotmail.com] >Sent: Friday, August 25, 2006 11:06 AM >To: Achstetter, Virginia A.; histonet@lists.utsouthwestern.edu >Subject: RE: [Histonet] TMA Book > >If you go to my website on creating tissuearrays I have several >resources on >the subject. Articles and videos. www.arrayworkshop.com > >And I do answer question through email or phone. I have given >workshops at two NSH meetings on tissue microarrays. > >Thom Jensen > > > >From: "Achstetter, Virginia A." > >To: > >CC: histonet@lists.utsouthwestern.edu > >Subject: [Histonet] TMA Book > >Date: Fri, 25 Aug 2006 06:50:32 -0400 > >MIME-Version: 1.0 > >Received: from swlx162.swmed.edu ([199.165.152.162]) by > >bay0-mc7-f18.bay0.hotmail.com with Microsoft SMTPSVC(6.0.3790.2444); > >Fri, > >25 Aug 2006 03:51:05 -0700 > >Received: from localhost ([127.0.0.1] helo=swlx162.swmed.edu)by > >swlx162.swmed.edu with esmtp (Exim 4.34)id 1GGZH8-0007ND-JR; Fri, 25 > >Aug > >2006 05:50:58 -0500 > >Received: from [199.165.152.166] (helo=swlx166.swmed.edu)by > >swlx162.swmed.edu with esmtp (Exim 4.34) id 1GGZH4-0007Mh-M4for > >histonet@lists.utsouthwestern.edu; Fri, 25 Aug 2006 05:50:54 -0500 > >Received: from pathology.swmed.edu ([129.112.48.201])by > >swlx166.swmed.edu with esmtp (Exim 4.44) id 1GGZH3-0001ZQ-Eufor > >histonet@lists.utsouthwestern.edu; Fri, 25 Aug 2006 05:50:54 -0500 > >Received: from swlx166.swmed.edu (199.165.152.166) by > >pathology.swmed.eduwith ESMTP (Eudora Internet Mail Server 3.1.5) for > >; Fri, 25 Aug 2006 05:30:03 -0500 > >Received: from lewisjr.afip.osd.mil ([198.97.78.51])by > >swlx166.swmed.edu with esmtp (Exim 4.44) id 1GGZH2-0001Z4-F1for > >histonet@pathology.swmed.edu; Fri, 25 Aug 2006 05:50:53 -0500 > >Received: from lewis.afip.osd.mil ([10.90.0.50]) by > >lewisjr.afip.osd.mil withMicrosoft SMTPSVC(6.0.3790.1830); Fri, 25 > >Aug > >2006 06:50:32 -0400 > >X-Message-Info: LsUYwwHHNt1vKQpZNB84SXxJqwy79yS2ZFneudwyjvA= > >X-MimeOLE: Produced By Microsoft Exchange V6.5 > >Content-class: urn:content-classes:message > >X-MS-Has-Attach: X-MS-TNEF-Correlator: Thread-Topic: TMA Book > >Thread-Index: AcbINEnXeEpWcwXvSt6AVgxmtG7HjQ== > >X-OriginalArrivalTime: 25 Aug 2006 10:50:32.0609 > >(UTC)FILETIME=[49A5B910:01C6C834] > >X-Scan-Signature: 18b8f45f5f122038853a7119e027f2bd > >X-Content-Filtered-By: Mailman/MimeDel 2.1.5 > >X-BeenThere: histonet@lists.utsouthwestern.edu > >X-Mailman-Version: 2.1.5 > >Precedence: list > >List-Id: For the exchange of information pertaining to > >histotechnology andrelated fields > >List-Unsubscribe: > >, > > >> > >List-Archive: > >List-Post: > >List-Help: > > > >List-Subscribe: > >, >is tonet-request@lists.utsouthwestern.edu?subject=subscribe> > >Errors-To: histonet-bounces@lists.utsouthwestern.edu > >X-Scan-Signature: 2ab8d6fe94b74561233a4cdd2e311ef7 > >X-SA-Exim-Connect-IP: 127.0.0.1 > >X-SA-Exim-Mail-From: histonet-bounces@lists.utsouthwestern.edu > >X-Spam-Checker-Version: SpamAssassin 2.64 (2004-01-11) on > >swlx162.swmed.edu > >X-Spam-Level: X-Spam-Status: No, hits=0.0 required=5.5 tests=none > >autolearn=no version=2.64 > >X-SA-Exim-Version: 4.0 (built Wed, 07 Apr 2004 12:14:41 -0500) > >X-SA-Exim-Scanned: Yes (on swlx162.swmed.edu) > >Return-Path: histonet-bounces@lists.utsouthwestern.edu > > > >Are there any good books out on how to prepare a tma block, trouble > >shooting, cutting techniques etc. > > > >Ginny Achstetter > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From laurie.colbert <@t> huntingtonhospital.com Fri Aug 25 15:38:00 2006 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Fri Aug 25 15:38:08 2006 Subject: [Histonet] Shipping Specimens Message-ID: <0BE6ADFAE4E7E04496BF21ABD346628005653CED@EXCHANGE1.huntingtonhospital.com> Is there anyone out there who can provide me with information, protocols, references, etc. on the shipping of specimens? I am interested not only in shipping requirements for the specimens itself, but also for the requirements for formalin and dry ice. I am in California, so I'm especially interested in the state requirements, which I hear are more strict than federal?? Laurie Colbert Huntington Memorial Hospital From rjbuesa <@t> yahoo.com Fri Aug 25 17:24:01 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Fri Aug 25 17:24:06 2006 Subject: [Histonet] Shipping Specimens In-Reply-To: <0BE6ADFAE4E7E04496BF21ABD346628005653CED@EXCHANGE1.huntingtonhospital.com> Message-ID: <20060825222401.54462.qmail@web61222.mail.yahoo.com> FedEx, as well as UPS, have their own protocols you have to comply with and can be used as your own requirements. Ren? J. Laurie Colbert wrote: Is there anyone out there who can provide me with information, protocols, references, etc. on the shipping of specimens? I am interested not only in shipping requirements for the specimens itself, but also for the requirements for formalin and dry ice. I am in California, so I'm especially interested in the state requirements, which I hear are more strict than federal?? Laurie Colbert Huntington Memorial Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. From steve.penney <@t> hotmail.com Fri Aug 25 17:53:53 2006 From: steve.penney <@t> hotmail.com (Steve Penney) Date: Fri Aug 25 17:54:01 2006 Subject: [Histonet] Do you know a good supplier for biopsy cassettes ? Message-ID: Hi I'd like to thank you for all your suggestions. Because of you, I contact many suppliers and I found my product at a better price. The product code is M503-2 and it's manufactured by Simport. One of there distributor sell a case of 1000 for $206. I think I can't tell you who he's selling it because we cannot do any kind of publicity here and it's good to respect that if we don't want to get ''invade'' by publicuty and promotion. Have a great week-end and thank you for your help P.S. Send me an e-mail if you are curious, I saw other cassettes models at low prices. _________________________________________________________________ Play Q6 for your chance to WIN great prizes. http://q6trivia.imagine-live.com/enca/landing From jnocito <@t> satx.rr.com Fri Aug 25 20:25:35 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Fri Aug 25 20:25:40 2006 Subject: [Histonet] Wearing gloves while cutting sections References: Message-ID: <007c01c6c8ae$88593450$4a624542@yourxhtr8hvc4p> thank you. Enjoy the weekend ----- Original Message ----- From: "Janice Mahoney" To: ; Sent: Friday, August 25, 2006 6:52 AM Subject: Re: [Histonet] Wearing gloves while cutting sections I've been in this field for over 30 years and have never heard of Hank solution. Do I want to drink it? And Joe, if anyone out there in Histoland ever questioned whether or not you are a nut, they now know. >>> "Rebecca Barnhart" 08/25/2006 6:45 AM >>> Wow! I thought it was weird when I once witnessed a pathologist drink Hank solution to determine if it was similar to a drink. Of course the Hank was fresh. That experience explained a lot about that pathologist. >>> "Joe Nocito" 8/24/2006 7:42:04 PM >>> Lick the block!? Damn, I thought I was the only one doing that. I used to do that with Pap smears. "This one is negative, negative, negative, positive, negative, oooh, this has HPV". Y'all didn't think I'd let this go by without a comment, did you? I love Fridays!!! Ooops, it's only Thursday. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Rene J Buesa" To: "LeVier, Rebecca J" ; "Scott, Allison D" ; Sent: Thursday, August 24, 2006 3:21 PM Subject: RE: [Histonet] Wearing gloves while cutting sections > Rebecca: > I think that you should expalin your thoughtful and illustrious commettee > that once the tissue is fixed and processed (unless it is KJ disease case) > you can lick the block. > By the way, I once did just that in front of the safety officer of our > lab (she left me alone for ever. I still don't know if she left me alone > because she was convinced or because she thought that I was completely out > of my mind; it worked!). > Ren? J. > > "LeVier, Rebecca J" wrote: > I am not sure that the committee fully understood what the process was. > Could they have been looking at the wrong thing. Did they really mean to > site for this or were they just having a misunderstanding? If it were > me, I would try to get together with them to talk. For me, it is almost > impossible for me to grab a ribbon with gloves on. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, > Allison D > Sent: Thursday, August 24, 2006 3:07 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Wearing gloves while cutting sections > > While I was on vaction, the safety committee came around and did rounds > in the lab. They sited us for not wearing gloves while cutting > sections. My techs tried to explain to them that we did not have to > wear gloves, only when cutting frozen sections or while handling fresh > tissue. I have never worn gloves while cutting paraffin sections. Now > my manager wants me to write a policy in regards to not wearing gloves. > Does anyone have anything like this in place that they would be willing > to share with me. Your help would be greatly appreciated. > > Allison Scott HT(ASCP) > Histology Supervisor > LBJ Hospital > Houston, Texas 77026 > > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify the > sender by return e-mail and delete this e-mail and any attachments from > your computer system. > > To the extent the information in this e-mail and any attachments contain > protected health information as defined by the Health Insurance > Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR > Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is > confidential and/or privileged. This e-mail may also be confidential > and/or privileged under Texas law. The e-mail is for the use of only > the individual or entity named above. If you are not the intended > recipient, or any authorized representative of the intended recipient, > you are hereby notified that any review, dissemination or copying of > this e-mail and its attachments is strictly prohibited. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of intended recipient(s) and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by reply e-mail and > destroy all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Get your own web address for just $1.99/1st yr. We'll help. Yahoo! Small > Business. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.405 / Virus Database: 268.11.5/426 - Release Date: 8/23/2006 > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.405 / Virus Database: 268.11.6/427 - Release Date: 8/24/2006 From CrochiereSteve <@t> aol.com Sat Aug 26 12:55:14 2006 From: CrochiereSteve <@t> aol.com (CrochiereSteve@aol.com) Date: Sat Aug 26 12:55:25 2006 Subject: [Histonet] Job Opening Message-ID: <55a.4bfb9d00.3221e502@aol.com> Greetings, We have and opening for an experienced histo tech with IHC and routine histology background. We have a privately owned laboratory located in the medical center. We handle hospital and outpatient cases. Annual volume >20k cases. Responsibilities would included processing, embedding, microtomy, special stains (mostly automated) and H&E staining (automated) as well as IHC (automated). Contact for more information: DR Shirin Nash (Shirin.Nash@sphs.com) Steve Crochiere (Steve.Crochiere@sphs.com) Thank you Steven M. Crochiere, HT(ASCP) Histology Supervisor LifePath Partners @ Mercy Medical Center Springfield, MA 01104 (413) 748-9541 From AnthonyH <@t> chw.edu.au Sun Aug 27 18:07:16 2006 From: AnthonyH <@t> chw.edu.au (Tony Henwood) Date: Sun Aug 27 18:08:42 2006 Subject: [Histonet] question about sirius red stain Message-ID: Several thoughts: Why stain for so long? Try shortening the time to even a few minutes. The yellow is probably leaching out in the acid water rinse. Try rinsing in ethanol before clearing and mounting. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC) Laboratory Manager & Senior Scientist The Children's Hospital at Westmead, Locked Bag 4001, Westmead, 2145, AUSTRALIA. Tel: 612 9845 3306 Fax: 612 9845 3318 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Xilong Li Sent: Saturday, 26 August 2006 3:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] question about sirius red stain Hi, All, I stain collagen of frozen muscle section using regular sirius red staining protocol(0.5g sirius red F3B in 500ml saturated picric acid, section were stained 1h in solution, then wash in 0.5% acidified water shortly). The background of images was all red under ordinary microscopy, but not yellow as expected, collagen can be seen barely with dark red. I extended washing time in acidified water, but no improvement was gotten. I just wonder any one had similar experience and how to improve it? Thanks in advance. Dr. Xilong Li Hypertension Division, Internal Medicine University of Texas Southwestern Medical Center 5323 Harry Hiness Blvd-J4.142 Dallas, TX 75390 Tel: 214-648-9966(L) Fax: 214-648-7902 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, the Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************** From melissa.mazan <@t> tufts.edu Sun Aug 27 20:39:12 2006 From: melissa.mazan <@t> tufts.edu (Melissa Mazan) Date: Sun Aug 27 20:39:20 2006 Subject: [Histonet] tissues falling off In-Reply-To: <200608241610.k7OGAjPA016370@mail-proofpoint-2a.usg.tufts.edu> References: <200608241610.k7OGAjPA016370@mail-proofpoint-2a.usg.tufts.edu> Message-ID: <44F24940.6090008@tufts.edu> Tanni - I had the same problem - my citrate buffer was too alkaline - pH of 6 made everything work fine. Melissa histonet-request@lists.utsouthwestern.edu wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-request@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-owner@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Looking for a new vendor for Amyloid P Antibody (Nava, Josefa) > 2. Disposal of surgical specimens (Linda Stirpe) > 3. eNOS evaluation (AGrobe2555@aol.com) > 4. Re: Disposal of surgical specimens (Rene J Buesa) > 5. diener pay (Spoon, Victoria) > 6. RE: Histonet Digest, Vol 33, Issue 28 (Margaryan, Naira) > 7. Cost of consumables (tncperfect2@comcast.net) > 8. Rat Histology (Hector Mobine) > 9. Hydrogen peroxide sterilization (AGrobe2555@aol.com) > 10. Histo Position (Barbara Murray) > 11. Re: diener pay (Godsgalnow@aol.com) > 12. Re: Cost of consumables (Godsgalnow@aol.com) > 13. Re: Looking for a new vendor for Amyloid P Antibody > (Jennifer MacDonald) > 14. RE: Who cleans your mortuary? (Rosalba) > 15. unsubscribe (Lynda King) > 16. F4/80 (Helen Beard) > 17. p-0 fixation and embedding-correction (Vicki Hakim) > 18. RE: Disposal of surgical specimens (Molinari, Betsy) > 19. IHC - sections falling off! (Ahmed, T (Tanni)) > 20. Re: Disposal of surgical specimens (Janci Wellborn) > 21. RE: IHC - sections falling off! (Dawson, Glen) > 22. Refurbished instruments with CMD (Coleman Manufacturing) > 23. RE: Patient slide send-out (Richard Cartun) > 24. RE: Disposal of surgical specimens (Horn, Hazel V) > 25. Re: F4/80 (Gayle Callis) > 26. RE: Refurbished instruments with CMD (Jes Strong) > 27. Re: Cost of consumables (Rene J Buesa) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Wed, 23 Aug 2006 12:50:30 -0500 > From: "Nava, Josefa" > Subject: [Histonet] Looking for a new vendor for Amyloid P Antibody > To: > Message-ID: > <2C515C1049EAF5459EFD8C9B929078A401DA13A6@phdex03.txhealth.org> > Content-Type: text/plain; charset="us-ascii" > > Hello, > > Can someone tell me where I can order Amyloid P antibody. Dako and > Novocastra have discontinued it. I will appreciate any information you > can give me. I will be running the Amyloid P on a Ventana machine and > VBS Bond Max. Thank you. > > Josie > > > The information contained in this message and any attachments is intended only for the use of the individual or entity to which it is addressed, and may contain information that is PRIVILEGED, CONFIDENTIAL, and exempt from disclosure under applicable law. If you are not the intended recipient, you are prohibited from copying, distributing, or using the information. Please contact the sender immediately by return e-mail and delete the original message from your system. > > ------------------------------ > > Message: 2 > Date: Wed, 23 Aug 2006 10:54:51 -0700 (PDT) > From: Linda Stirpe > Subject: [Histonet] Disposal of surgical specimens > To: HistoNet Server > Message-ID: <20060823175451.94510.qmail@web54614.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > I know this subject has been previously discussed but > now it has become an issue at our facility. We are a > small hospital processing about 5,000 surgicals/year. > Currently, we dispose of the specimens with fixative > by placing them in biohazard containers and then > hauled off site. The safety committee now has concerns > that the specimen is a biohazard and the formain is a > hazardous waste and should be separated for disposal > purposes. We are interested in how other hospitals > handle this. Any suggestions would be greatly > appreciated. Thank you! > > Linda Stirpe, HT(ASCP) > Corning Hospital > Corning, NY > > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > > > ------------------------------ > > Message: 3 > Date: Wed, 23 Aug 2006 14:49:08 EDT > From: AGrobe2555@aol.com > Subject: [Histonet] eNOS evaluation > To: histonet@lists.utsouthwestern.edu > Message-ID: <57c.3e0afac.321dfd24@aol.com> > Content-Type: text/plain; charset="US-ASCII" > > Tahseen, > I have worked with eNOS in the past. Can you give me a bit more detail of > what you are looking for? Cellular localization, levels, NO production, > phosphorylation state, etc....? > Albert > > > ------------------------------ > > Message: 4 > Date: Wed, 23 Aug 2006 11:54:26 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Disposal of surgical specimens > To: Linda Stirpe , HistoNet Server > > Message-ID: <20060823185426.21282.qmail@web61218.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Linda: > We used to separate formalin from the tissue. Formalin was placed in large drums to be hauled away by a company we contracted. The tissues were incinerated at the hospital (something that probably you will not be able to do). Otherwise the tissue should be handled as any other biohazard sample within the lab. > Hope this will help you! > Ren? J. > > Linda Stirpe wrote: > I know this subject has been previously discussed but > now it has become an issue at our facility. We are a > small hospital processing about 5,000 surgicals/year. > Currently, we dispose of the specimens with fixative > by placing them in biohazard containers and then > hauled off site. The safety committee now has concerns > that the specimen is a biohazard and the formain is a > hazardous waste and should be separated for disposal > purposes. We are interested in how other hospitals > handle this. Any suggestions would be greatly > appreciated. Thank you! > > Linda Stirpe, HT(ASCP) > Corning Hospital > Corning, NY > > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Want to be your own boss? Learn how on Yahoo! Small Business. > > ------------------------------ > > Message: 5 > Date: Wed, 23 Aug 2006 15:22:06 -0400 > From: "Spoon, Victoria" > Subject: [Histonet] diener pay > To: > Message-ID: <415700FC732DE14491A3E39367834F7715AD6E@ex3.bassett.org> > Content-Type: text/plain; charset="iso-8859-1" > > What is the going rate of pay for dieners? > > Thanks in advance. > > > NOTICE OF CONFIDENTIALITY > This electronic message, including attachments, is for the sole use of the > named recipient and may contain confidential or privileged information > protected by New York State, and Federal regulations. Any unauthorized > review, use, disclosure, copying or distribution is strictly prohibited. > If you are not the intended recipient or have received this communication > in error please contact the sender or email.security@bassett.org and > destroy all copies of the original message. Thank you. > > > > ------------------------------ > > Message: 6 > Date: Wed, 23 Aug 2006 16:38:08 -0500 > From: "Margaryan, Naira" > Subject: [Histonet] RE: Histonet Digest, Vol 33, Issue 28 > To: > Message-ID: > <63B8B599DE283148B92E83C78B32C15D02E0DFEE@cmhexbe2.childrensmemorial.org> > > Content-Type: text/plain; charset="us-ascii" > > Me too, please :-) And, please, not only histology, but cancer pathology > as well!!! > > > > Thanks, > > Naira > > > > Naira V. Margaryan, D.V.M., Ph.D. > > Research Associate III > > Children's Memorial Research Center > > 2300 Children's Plaza, Box 222 > > Chicago, IL 60614-3394 > > Tel: 773-755-6570/ext-8 > > Fax: 773-755-6594 > > nmargaryan@childrensmemorial.org > > > > For Express Mail: > > CMRC, Room C.473 > > 2430 N. Halsted Street > > Chicago, IL 60614-4314 > > > > ------------------------------ > > > > Message: 10 > > Date: Tue, 22 Aug 2006 17:53:49 -0800 > > From: "Barbara Murray" > > Subject: [Histonet] On-line Histology Schools > > To: > > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > > > > > > > Greetings from Anchorage, Alaska > > Would someone please send me a list of on-line Histology Schools. > > Thanks in advance and have a great day! > > > > Barbara A. Murray, HT. (ASCP) > > The Alaska Native Medical Center > > Anchorage, Alaska > > > > ------------------------------ > > Message: 7 > Date: Wed, 23 Aug 2006 22:09:24 +0000 > From: tncperfect2@comcast.net > Subject: [Histonet] Cost of consumables > To: histonet@lists.utsouthwestern.edu > Message-ID: > <082320062209.25134.44ECD214000AC3560000622E2213575333CD9B0C0A009D0A9F0C029B@comcast.net> > > Content-Type: text/plain > > Hi Histonetters. > I need to know how much managers are spending on consumables that deal with 1000 or more specimens a month...give or take a few dollars? I just need a ball park figure for now. We do just the H&E routine stain. > If anyone can help me it would be very much appreciated. > Thanks, > Carolyn > > ------------------------------ > > Message: 8 > Date: Wed, 23 Aug 2006 18:16:46 -0400 > From: "Hector Mobine" > Subject: [Histonet] Rat Histology > To: > Message-ID: <200608232216.k7NMGkix025572@outgoing.mit.edu> > Content-Type: text/plain; charset="us-ascii" > > I am in the process of designing histological experiments of rat and mice > hearts and would be interested in a good description of rat heart excision > and anatomy. I have yet been unable to find one in medical textbooks, > reviews or protocols. Does anyone know of any good references, pictures, > books or protocols? Any help would be appreciated. > > > > Thanks, > > Hector > > > > > > Graduate Student > > Edelman Lab > > Biological Engineering Division > > Massachusetts Institute of Technology > > > > > > ------------------------------ > > Message: 9 > Date: Wed, 23 Aug 2006 18:27:22 EDT > From: AGrobe2555@aol.com > Subject: [Histonet] Hydrogen peroxide sterilization > To: histonet@lists.utsouthwestern.edu > Message-ID: <414.8c46d5a.321e304a@aol.com> > Content-Type: text/plain; charset="US-ASCII" > > Does anyone know of a company which will do H2O2 gas-phase sterilization on > a contract basis? Please feel free to contact me directly off-list. > Thanks, > Albert > > > ------------------------------ > > Message: 10 > Date: Wed, 23 Aug 2006 15:29:15 -0800 > From: "Barbara Murray" > Subject: [Histonet] Histo Position > To: > Message-ID: > Content-Type: text/plain; charset="iso-8859-1" > > > > Greetings from Anchorage, Alaska. > We currently have two openings in Histology @ The Alaska Native Medical > Center. Contact David Morrison, Laboratory Manager @ 907-729-1228 for > details and $$$$$$! > Have a great day! > > Barbara A. Murray, HT. (ASCP) > The Alaska Native Medical Center > Anchorage, Alaska 99508 > > > > > ------------------------------ > > Message: 11 > Date: Wed, 23 Aug 2006 20:56:22 EDT > From: Godsgalnow@aol.com > Subject: Re: [Histonet] diener pay > To: victoria.spoon@bassett.org, histonet@pathology.swmed.edu > Message-ID: <516.60e833f.321e5336@aol.com> > Content-Type: text/plain; charset="US-ASCII" > > I have always paid per case.....$250 - $300 per case. It doesn't matter if > it is a chest only or a full body, the pay is the same. > Roxanne > > > ------------------------------ > > Message: 12 > Date: Wed, 23 Aug 2006 21:01:19 EDT > From: Godsgalnow@aol.com > Subject: Re: [Histonet] Cost of consumables > To: tncperfect2@comcast.net, histonet@lists.utsouthwestern.edu > Message-ID: <37d.9d06a47.321e545f@aol.com> > Content-Type: text/plain; charset="US-ASCII" > > This is all so subjective. It depends on where you are located, how much > you pay for shipping, if at all. If you have a GPO or multiple vendors that > you use. Progressive or regressive staining? Gills, Harris, Clarifier or no? > Bluing or no? Plus slides, regular slides? Do you cut extras? Do you have > to pay for DI water? Do you have a specific brand of products you use? > > > Roxanne Soto > Lab Manager > Physicians RightPath > Tampa, Florida > 813-549-1050 > > > ------------------------------ > > Message: 13 > Date: Wed, 23 Aug 2006 20:32:12 -0700 > From: Jennifer MacDonald > Subject: Re: [Histonet] Looking for a new vendor for Amyloid P > Antibody > To: "Nava, Josefa" > Cc: histonet@pathology.swmed.edu > Message-ID: > > Content-Type: text/plain; charset="UTF-8" > > > I know that BioCare has it. You can also check Cell Marque. > > > > > -----hist To: > Sen Date: 08/23/2006 10:50AM Subject: [Histonet] Looking for a new vendor for Amyloid P Antibody > Hello, > Can someone tell me where I can order Amyloid and > Novocastra have discontinued &nb information you > can give me. I will be runn and > VBS Bond Max. &nb Josie > The information contained in this mes intended only for the use of the individual or addressed, and may contain information that is PRIVIL CONFIDENTIAL, and exempt from disclosure under applicable law. you are not the intended recipient, you are prohibited from copying, > dis immedi your system. > _______________________ 5F _______________________ > Hi Histonet@lists.utsouthwestern.edu > [1]htt > References > > 1. file://localhost/tmp/3D"http > > ------------------------------ > > Message: 14 > Date: Thu, 24 Aug 2006 15:39:39 +1000 > From: Rosalba > Subject: RE: [Histonet] Who cleans your mortuary? > To: 'Mike Kirby' , "Histonet (E-mail)" > > Message-ID: <01C6C793.83411BC0.zumbor@email.cs.nsw.gov.au> > Content-Type: text/plain; charset="us-ascii" > > Hi All, > The mortuary technicians who assist with the post-mortems clean the > mortuary after they have finished their cases. Each cleans their own work > area and there is a roster for cleaning the other general areas such as the > fridge etc. > Rosalba Zumbo > Supervisor Histology Dept > Department of Forensic Medicine > 42-50 Parramatta Rd > Glebe NSW 2037 > Australia > PH: 61 2 85847842 > FAX: 61 2 95664573 > > > > -----Original Message----- > From: Mike Kirby [SMTP:mike.kirby@nhls.ac.za] > Sent: Wednesday, 23 August 2006 7:45 PM > To: Histonet (E-mail) > Subject: [Histonet] Who cleans your mortuary? > > > Hi Guys. > > Question! Who cleans your mortuary? Swabbing the floors and tables, > cleaning the refrigeration area, sinks, windows, etc. Do you have > permanent, in-house trained Staff, or do you contract out to a commercial > company? > > M.Kirby > Johannesburg > South Africa. > > ************************************************************************ > ********** > The views expressed in this email are, unless otherwise stated, those of > the author and not those of the National Health Laboratory Services or its > management. The information in this e-mail is confidential and is intended > solely for the addressee. > Access to this e-mail by anyone else is unauthorized. If you are not the > intended recipient, any disclosure, copying, distribution or any action > taken or omitted in reliance on this, is prohibited and may be unlawful. > Whilst all reasonable steps are taken to ensure the accuracy and integrity > of information and data transmitted electronically and to preserve the > confidentiality thereof, no liability or responsibility whatsoever is > accepted if information or data is, for whatever reason, corrupted or does > not reach its intended destination. > > ************************************************************************ > *********** > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _____________________________________________________________________ > This email has been scanned for the Sydney South West Area Health Service > by the MessageLabs Email Security System. SSWAHS regularly monitors emails > and attachments to ensure compliance with the NSW Government's Electronic > Messaging Policy. > > "This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please destroy it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of the Sydney South West Area Health Service." > > > > ------------------------------ > > Message: 15 > Date: Thu, 24 Aug 2006 16:15:45 +1000 > From: "Lynda King" > Subject: [Histonet] unsubscribe > To: > Message-ID: <5E06BFED29594F4C9C5EBE230DE320C6114B5DBC@ewok.vsl.com.au> > Content-Type: text/plain; charset="iso-8859-1" > > > > Lynda King > Customer and Sales Administrator > > Vision BioSystems Ltd > 495 Blackburn Road > Mt Waverley VIC 3149 > Australia > > telephone: +61 3 9211 7400 > facsimile: +61 3 9211 7401 > > www.vision-bio.com > > IMPORTANT - This email and any attachments may be confidential. Any retransmissions, dissemination or other use of these materials by persons or entities other than the intended recipient is prohibited. If received in error, please contact us and delete all copies. Before opening or using attachments, check them for viruses and defects. Our liability is limited to resupplying any affected attachments. [Any representations or opinions expressed in this email are those of the individual sender, and not necessarily those of Vision Systems Limited]. > > > > > ------------------------------ > > Message: 16 > Date: Thu, 24 Aug 2006 03:18:32 -0500 > From: "Helen Beard" > Subject: [Histonet] F4/80 > To: mobine@MIT.EDU, histonet@lists.utsouthwestern.edu > Message-ID: > Content-Type: text/plain; format="flowed" > > > I would be interested in hearing from anyone who has used Sertec F4/80 > to do IHC for microglia in pfa fixed mouse brains. I have lovely > staining in my kupfer cells (control) but only staining of what > appears to be neurones in the brain. A protocol would be greatly > appreciated. > > Thanks in advance > > Helen > > > ------------------------------ > > Message: 17 > Date: Thu, 24 Aug 2006 09:52:28 GMT > From: Vicki Hakim > Subject: [Histonet] p-0 fixation and embedding-correction > To: Gayle Callis , histonet > > Message-ID: > Content-Type: text/plain; charset=utf-8 > > sorry for the abbreviation, p-0 means p- zero (immediately after birth) > thanks again > vicki > ----- Original Message ----- > From: Gayle Callis > Date: Wednesday, August 23, 2006 19:03 > Subject: Re: [Histonet] p-0 fixation and embedding > To: Vicki Hakim > >>Define what P-O mouse brains means, please. >> >>At 08:26 AM 8/23/2006, you wrote: >> >>>hi all >>>we are interested in a protocol for fixation and embedding for >> >>P-0 mouse >> >>>brains. if u have a recommendation on 1) the preferable >> >>fixative (PFA or >> >>>formalin) and the specific times for fixation with each >> >>substance 2) time >> >>>in the melted paraffin 3) xylene or toluene >>>thanks in advance >>>vicki >>> >>> >>> ?????? >>>_______________________________________________ >>>Histonet mailing list >>>Histonet@lists.utsouthwestern.edu >>>http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >>Gayle Callis >>MT,HT,HTL(ASCP) >>Research Histopathology Supervisor >>Veterinary Molecular Biology >>Montana State University - Bozeman >>PO Box 173610 >>Bozeman MT 59717-3610 >>406 994-6367 >>406 994-4303 (FAX) >> >> >>??? > > > > ------------------------------ > > Message: 18 > Date: Thu, 24 Aug 2006 06:09:26 -0500 > From: "Molinari, Betsy" > Subject: RE: [Histonet] Disposal of surgical specimens > To: > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > Linda, > We do the same here. Our tissue is stored in "seal a meal" Heavy Duty Kapak bags until the investigator is ready to let it go. Then we cut a small corner off the bag, dump the formalin in a waste container, reseal the bag and put it into a biohazard bag for the hospital to dispose of. > > Betsy Molinari HT (ASCP) > Texas Heart Institute > Cardiovascular Pathology > 6770 Bertner Ave. > Houston,TX 77030 > 832-355-6524 > 832-355-6812 (fax) > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Wednesday, August 23, 2006 1:54 PM > To: Linda Stirpe; HistoNet Server > Subject: Re: [Histonet] Disposal of surgical specimens > > Linda: > We used to separate formalin from the tissue. Formalin was placed in large drums to be hauled away by a company we contracted. The tissues were incinerated at the hospital (something that probably you will not be able to do). Otherwise the tissue should be handled as any other biohazard sample within the lab. > Hope this will help you! > Ren? J. > > Linda Stirpe wrote: > I know this subject has been previously discussed but > now it has become an issue at our facility. We are a > small hospital processing about 5,000 surgicals/year. > Currently, we dispose of the specimens with fixative > by placing them in biohazard containers and then > hauled off site. The safety committee now has concerns > that the specimen is a biohazard and the formain is a > hazardous waste and should be separated for disposal > purposes. We are interested in how other hospitals > handle this. Any suggestions would be greatly > appreciated. Thank you! > > Linda Stirpe, HT(ASCP) > Corning Hospital > Corning, NY > > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Want to be your own boss? Learn how on Yahoo! Small Business. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 19 > Date: Thu, 24 Aug 2006 12:15:55 +0100 > From: "Ahmed, T \(Tanni\)" > Subject: [Histonet] IHC - sections falling off! > To: > Message-ID: <11DBF8AFF1DC0F4A9B51A23F8E5BDA33799067@mksn83.d50.intra> > Content-Type: text/plain; charset="us-ascii" > > Dear All, > > I am trying to save sections of canine mammary tissue from falling off > in antigen retrieval solution in a microwave technique (have also tried > half power microwaving). I am using normal superfrost slides and leaving > them to incubate overnight. The sections are fine after dewaxing - > therefore I believe the type of slide used whether it be superfrost or > positive charged polysaline slides should not matter? My other option is > maybe to use non heat-treatment methods of enzyme digestion such as > trypsin. Is there anyone who has any ideas to prevent sections falling > off during the microwave retrieval step? > > Any suggestions to improve are welcome. > > Thanks in advance, > > Tanni > > -------------------------------------- > This message, including attachments, is confidential and may be privileged. > If you are not an intended recipient, please notify the sender then delete > and destroy the original message and all copies. You should not copy, forward > and/or disclose this message, in whole or in part, without permission of > the sender. > -------------------------------------- > > ------------------------------ > > Message: 20 > Date: Thu, 24 Aug 2006 08:18:03 -0400 > From: "Janci Wellborn" > Subject: Re: [Histonet] Disposal of surgical specimens > To: histonet@pathology.swmed.edu, lpstirpe@yahoo.com, > rjbuesa@yahoo.com > Message-ID: > Content-Type: text/plain; charset=iso-8859-1 > > Linda, > We separate the formalin from the tissue. The formalin is then neutralized and solidified with product called ISOSORB. The tissue and solid formalin waste is then doubled bagged and disposed of as any other biohazard sample. Our limbs are incinerated by a local mortuary. > > Janci Wellborn, HTL, BS, BSeD > Dunes Medical Lab > Dakota Dunes, SD > > >>>>"Rene J Buesa" 8/23/2006 1:54 PM >>> > > Linda: > We used to separate formalin from the tissue. Formalin was placed in large drums to be hauled away by a company we contracted. The tissues were incinerated at the hospital (something that probably you will not be able to do). Otherwise the tissue should be handled as any other biohazard sample within the lab. > Hope this will help you! > Ren? J. > > Linda Stirpe wrote: > I know this subject has been previously discussed but > now it has become an issue at our facility. We are a > small hospital processing about 5,000 surgicals/year. > Currently, we dispose of the specimens with fixative > by placing them in biohazard containers and then > hauled off site. The safety committee now has concerns > that the specimen is a biohazard and the formain is a > hazardous waste and should be separated for disposal > purposes. We are interested in how other hospitals > handle this. Any suggestions would be greatly > appreciated. Thank you! > > Linda Stirpe, HT(ASCP) > Corning Hospital > Corning, NY > > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Want to be your own boss? Learn how on Yahoo! Small Business. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > ------------------------------ > > Message: 21 > Date: Thu, 24 Aug 2006 08:14:10 -0500 > From: "Dawson, Glen" > Subject: RE: [Histonet] IHC - sections falling off! > To: "Ahmed, T \(Tanni\)" , > > Message-ID: > > Content-Type: text/plain; charset="iso-8859-1" > > Tanni, > > Don't underestimate the power of a slide with extra adhesive properties. Try out Goldfrost Plus Slides or the Silanized slides from Dako. > > Also, try extending the time that you dry your slides before you start your procedure. > > Keep in mind that enzyme digestion can make tissue sections fall off as well. > > Good Luck, > > Glen Dawson BS, HT & QIHC (ASCP) > IHC Manager > Milwaukee, WI > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Ahmed, T > (Tanni) > Sent: Thursday, August 24, 2006 5:16 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] IHC - sections falling off! > > > Dear All, > > I am trying to save sections of canine mammary tissue from falling off > in antigen retrieval solution in a microwave technique (have also tried > half power microwaving). I am using normal superfrost slides and leaving > them to incubate overnight. The sections are fine after dewaxing - > therefore I believe the type of slide used whether it be superfrost or > positive charged polysaline slides should not matter? My other option is > maybe to use non heat-treatment methods of enzyme digestion such as > trypsin. Is there anyone who has any ideas to prevent sections falling > off during the microwave retrieval step? > > Any suggestions to improve are welcome. > > Thanks in advance, > > Tanni > > -------------------------------------- > This message, including attachments, is confidential and may be privileged. > If you are not an intended recipient, please notify the sender then delete > and destroy the original message and all copies. You should not copy, forward > and/or disclose this message, in whole or in part, without permission of > the sender. > -------------------------------------- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 22 > Date: Thu, 24 Aug 2006 06:18:31 -0700 (PDT) > From: Coleman Manufacturing > Subject: [Histonet] Refurbished instruments with CMD > To: histonet@lists.utsouthwestern.edu > Message-ID: <20060824131831.20959.qmail@web37614.mail.mud.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Tto provide every laboratory and hospital with the opportunity to enjoy the many benefits of our products, CMD is delighted to offer factory-refurbished instruments at significantly discounted prices. All of these instruments are backed by a full one-year parts and service warranty. Offer subject to availability. > > Equipment of the following types: > Cover Slippers > Cryostats > Microtomes > Embedding centers > Tissue Processors > Stainers > Laboratory Microwave Systems > You can request more information on the available equipment by contacting our office at: > > Coleman Manufacturing & Design > 2852 Old Airport Road > Winnsboro, SC 29180 > > 803.633.2124 office > 803.635.9401 fax > Coleman_Manufacturing@yahoo.com > > > > > ------------------------------ > > Message: 23 > Date: Thu, 24 Aug 2006 09:25:00 -0400 > From: "Richard Cartun" > Subject: RE: [Histonet] Patient slide send-out > To: "Betsy Molinari" , > > Message-ID: <44ED706C020000770000187A@gwmail.harthosp.org> > Content-Type: text/plain; charset=US-ASCII > > Today, patients are more educated regarding health care issues and no > longer settle for what their physician tells them. Here in the East, > many patients want their diagnoses confirmed by major cancer centers in > New York and Boston. Also, they may be looking for alternative > treatments. > > Richard > > Richard W. Cartun, Ph.D. > Director, Immunopathology & Histology > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-0174 Fax > > >>>>"Molinari, Betsy" 08/23/06 6:37 AM >>>> > > Just curious..why this increase in patients asking for a second > opinion? > > Betsy Molinari HT (ASCP) > Texas Heart Institute > Cardiovascular Pathology > 6770 Bertner Ave. > Houston,TX 77030 > 832-355-6524 > 832-355-6812 (fax) > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Zajic > Kari > Sent: Tuesday, August 22, 2006 2:05 PM > To: Richard Cartun; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Patient slide send-out > > Richard, you are not alone! We have also seen an increase in patient > and > physician requests for "send-outs". I am not sure of charging, but I > have made a "send-out" policy for our department that all requests be > put in writing (scripts for physicians)and faxed,inform the > patient/office that it will at least be 48 hours before it can be sent > out, they must provide a shipping service account (FEDEX, UPS) or > physically pick up the slides. We have also been being charged for > these > consults by the other facility so we also inform them that if the > insurance cannot be charged, they are responsible for the bill. It > seems > to work but we do run into some problems now and again. If they cannot > provide an overnight shipping service, we will USPS mail them > certified > but that seems to take too long for their liking (here it's around a > week). > Not having enough staff to handle the sendouts is always a problem, > hence the 48 hours..helps slightly. > > Kari :) > > Kari Marie Zajic HTL,MLT > Histology Supervisor > Palms West Hospital > Pathology Department > 13001 State Road Eighty > Loxahatchee, Florida 33470 > phone: (561)798-6036 > telefax: (561)753-4298 > voicemail: (561)753-4299 > pager: (561)610-4949 > email: Kari.Zajic@HCAHealthcare.com > > This email and any files transmitted with it may contain PRIVILEGED or > CONFIDENTIAL > information and may be read or used only by the intended recipient. If > you are not the intended recipient of the email or any of its > attachment, please be advised that you have received this email in > error > and that any use, dissemination, distribution, forwarding, printing, > or > copying of this email or any attached files is strictly prohibited. If > you have received this email in error, please immediately purge it and > all attachments and notify the sender by reply email or contact the > sender at the number listed. > > > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Richard > Cartun > Sent: Tuesday, August 22, 2006 2:49 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Patient slide send-out > > > Our Anatomic Pathology Office is overwhelmed with requests from > patients > asking for their pathology slides to be sent to another medical > institution (for second opinion, additional surgery or therapy, etc.). > > Is it legal to charge patients for this service? We don't have the > personnel to handle these requests in a timely fashion and we can no > longer afford to "eat" the shipping costs. Thank you. > > Richard > > Richard W. Cartun, Ph.D. > Director, Immunopathology & Histology > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 > (860) 545-0174 Fax > > > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > ------------------------------ > > Message: 24 > Date: Thu, 24 Aug 2006 09:02:11 -0500 > From: "Horn, Hazel V" > Subject: RE: [Histonet] Disposal of surgical specimens > To: "Rene J Buesa" , "Linda Stirpe" > , "HistoNet Server" > Message-ID: > <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6D53@EMAIL.archildrens.org> > Content-Type: text/plain; charset=iso-8859-1 > > We pour the formalin off of the specimens. The specimens are discarded and hauled off by the waste company. The spent formalin is also taken by another waste company for disposal. It's a pain. > > Hazel Horn > Hazel Horn, HT/HTL (ASCP) > Supervisor of Histology > Arkansas Children's Hospital > 800 Marshall Slot 820 > Little Rock, AR 72202 > > phone 501.364.4240 > fax 501.364.3912 > > visit us on the web at: www.archildrens.org > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa > Sent: Wednesday, August 23, 2006 1:54 PM > To: Linda Stirpe; HistoNet Server > Subject: Re: [Histonet] Disposal of surgical specimens > > Linda: > We used to separate formalin from the tissue. Formalin was placed in large drums to be hauled away by a company we contracted. The tissues were incinerated at the hospital (something that probably you will not be able to do). Otherwise the tissue should be handled as any other biohazard sample within the lab. > Hope this will help you! > Ren? J. > > Linda Stirpe wrote: > I know this subject has been previously discussed but > now it has become an issue at our facility. We are a > small hospital processing about 5,000 surgicals/year. > Currently, we dispose of the specimens with fixative > by placing them in biohazard containers and then > hauled off site. The safety committee now has concerns > that the specimen is a biohazard and the formain is a > hazardous waste and should be separated for disposal > purposes. We are interested in how other hospitals > handle this. Any suggestions would be greatly > appreciated. Thank you! > > Linda Stirpe, HT(ASCP) > Corning Hospital > Corning, NY > > > __________________________________________________ > Do You Yahoo!? > Tired of spam? Yahoo! Mail has the best spam protection around > http://mail.yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Want to be your own boss? Learn how on Yahoo! Small Business. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------------------------------------------------------ > The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. > ============================================================================== > > > > > ------------------------------ > > Message: 25 > Date: Thu, 24 Aug 2006 09:09:13 -0600 > From: Gayle Callis > Subject: Re: [Histonet] F4/80 > To: "Helen Beard" , > Histonet@lists.utsouthwestern.edu > Message-ID: > <6.0.0.22.1.20060824090747.01b39ca0@gemini.msu.montana.edu> > Content-Type: text/plain; charset="us-ascii"; format=flowed > > This has been answered on Histonet several times, just go to Archives and > type in F4/80. Barb Wright is one who has given good info on using this > antibody from Serotec. There will be other excellent replies also. > > At 02:18 AM 8/24/2006, you wrote: > > >> I would be interested in hearing from anyone who has used Sertec F4/80 >> to do IHC for microglia in pfa fixed mouse brains. I have lovely >> staining in my kupfer cells (control) but only staining of what >> appears to be neurones in the brain. A protocol would be greatly >> appreciated. >> >> Thanks in advance >> >> Helen >>_______________________________________________ >>Histonet mailing list >>Histonet@lists.utsouthwestern.edu >>http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Gayle Callis > MT,HT,HTL(ASCP) > Research Histopathology Supervisor > Veterinary Molecular Biology > Montana State University - Bozeman > PO Box 173610 > Bozeman MT 59717-3610 > 406 994-6367 > 406 994-4303 (FAX) > > > > > > ------------------------------ > > Message: 26 > Date: Thu, 24 Aug 2006 11:01:40 -0500 > From: "Jes Strong" > Subject: RE: [Histonet] Refurbished instruments with CMD > To: > Message-ID: <003001c6c796$96c2abc0$0200a8c0@Jes> > Content-Type: text/plain; charset="us-ascii" > > This guy just doesn't quit! I would hope that all Histonetters would boycott > supporting his company until he stops his blatant misuse of the Histonet for > personal marketing purposes. There are many very good companies out there > that respect the intent of the Histonet and can provide you with quality > products at competitive prices. > > Jes Strong > > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Coleman > Manufacturing > Sent: Thursday, August 24, 2006 8:19 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Refurbished instruments with CMD > > Tto provide every laboratory and hospital with the opportunity to enjoy the > many benefits of our products, CMD is delighted to offer factory-refurbished > instruments at significantly discounted prices. All of these instruments are > backed by a full one-year parts and service warranty. Offer subject to > availability. > > Equipment of the following types: > Cover Slippers > Cryostats > Microtomes > Embedding centers > Tissue Processors > Stainers > Laboratory Microwave Systems > You can request more information on the available equipment by contacting > our office at: > > Coleman Manufacturing & Design > 2852 Old Airport Road > Winnsboro, SC 29180 > > 803.633.2124 office > 803.635.9401 fax > Coleman_Manufacturing@yahoo.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > ------------------------------ > > Message: 27 > Date: Thu, 24 Aug 2006 09:05:32 -0700 (PDT) > From: Rene J Buesa > Subject: Re: [Histonet] Cost of consumables > To: tncperfect2@comcast.net, histonet@lists.utsouthwestern.edu > Message-ID: <20060824160532.39614.qmail@web61212.mail.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > Carolyn: > I just finished a paper on costs that I am sending you under separate cover. > The cost are per unit, just multiply them by your needs. > Ren? J. > > tncperfect2@comcast.net wrote: > Hi Histonetters. > I need to know how much managers are spending on consumables that deal with 1000 or more specimens a month...give or take a few dollars? I just need a ball park figure for now. We do just the H&E routine stain. > If anyone can help me it would be very much appreciated. > Thanks, > Carolyn > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Do you Yahoo!? > Get on board. You're invited to try the new Yahoo! Mail Beta. > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 33, Issue 30 > **************************************** -- Melissa R. Mazan, DVM, Diplomate ACVIM Associate Professor of Large Animal Medicine Director of Sports Medicine Cummings School of Veterinary Medicine Tufts University 200 Westborough Road North Grafton, MA 01536 Tel: 508-839-5395 Fax: 508-839-7922 Email: melissa.mazan@tufts.edu From kappeler <@t> patho.unibe.ch Mon Aug 28 00:41:49 2006 From: kappeler <@t> patho.unibe.ch (Andi Kappeler) Date: Mon Aug 28 00:42:30 2006 Subject: [Histonet] Immuno - Her2Neu References: Message-ID: <00a101c6ca64$ba7fdd00$27955c82@patho.unibe.ch> Try http://www.ecacc.org.uk/, they have just started to market slides that contain 4 different cell lines, each with a different level of expression of Her2. We were given the opportunity to test them and they worked well in our hands, however they are not exactly cheap. Currently there is an ad for Her2 control slides on the left part of the ECACC homepage --> click. Andi Kappeler Institute of Pathology, University of Bern, Switzerland ----- Original Message ----- From: "Paula Wilder" To: Sent: Friday, August 25, 2006 4:52 PM Subject: [Histonet] Immuno - Her2Neu > Has anyone found a commercial control for Her2/Neu that includes 1+, 2+, > and 3+ tissue all on one slide? Dako will not sell theirs separately, and > CellMarque has only 3+ on their control slides. > > Thanks so much! > Paula Wilder > St. Joseph Medical Center > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > From histo007 <@t> hotmail.com Mon Aug 28 08:38:29 2006 From: histo007 <@t> hotmail.com (Jim Ball) Date: Mon Aug 28 08:38:34 2006 Subject: [Histonet] Breast Lecture Message-ID: I have been ask to present a lecture on breast Biopsies to the West Virginia Radiology Society. I would like to give them some insight into the proper handling of the biopsies before they are brought to the histology department. This will cover the most mundane items such as proper Demographics, proper labeling of the specimen, types of transport media, etc........, but while I was discussing this with a pathologist he mentioned it would be nice if the orders stated if the patient were pregnant or not. He was well aware he could look it up in the history, but felt it could save time if it were stated on the request. This got me to thinking and my thoughts turned to histonet. So if any of you would like to share some time saving protocols and procedures that I could review for the lecture please forward them to me and the NET. The title of the lecture will be "It's All UP Front". I only include the title in hopes it will jog a few brain cells and you'll think of other areas that might contribute to the histology department being able to improve patient care if everything is done right in the beginning. I would be very interested in knowing how you anticipate or should I say plan for the worst case scenario, since we usually have very small samples when it comes to needle biopsies. I am looking for ways that maxamizes the sample for not only testing today, but also testing in the future. From TJasper <@t> smdc.org Mon Aug 28 09:15:47 2006 From: TJasper <@t> smdc.org (Jasper, Thomas G.) Date: Mon Aug 28 09:16:09 2006 Subject: [Histonet] Breast Lecture In-Reply-To: Message-ID: <1A9F2A6C5762524799A816F1F09744CF1E0B7F@SCREECH.ntcampus.smdc.org> Hey Jim, I think this is a very good idea. Your comments about pregnancy made me think of "Clinical History/Information". It is very important to include this information for the Pathology department and too many times (in my opinion) it is excluded. That applies to any tissue type as well. Another thing is legible handwriting, unless your system is totally electronic and you are not receiving handwritten requisition forms. It has boggled my mind for years at how little importance physicians (in general) place on clearly written information. To me this is a big patient care issue. Bad handwriting at the very least can slow down the processing of patient cases and at worst compromise their treatment. If you can't write legibly try printing. Again, this applies to all pathology specimens. One other issue we've encountered is cramming too much specimen into too small a container. This is especially bad after "normal" working hours or over a weekend. There is no way anyone can expect proper fixation to occur in this situation. Proper fixation is ultimately important for all specimens. In breast cases it is particularly so as there are more and more immunohistochemical requests for these patients. I'm sure I'm not telling you anything you don't already know or that most folks monitoring the Histonet don't know...but you asked. Hopes this helps you some. Regards, Thomas Jasper HT (ASCP) BAS Anatomic Pathology Supervisor SMDC Duluth, MN -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Jim Ball Sent: Monday, August 28, 2006 8:38 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Breast Lecture I have been ask to present a lecture on breast Biopsies to the West Virginia Radiology Society. I would like to give them some insight into the proper handling of the biopsies before they are brought to the histology department. This will cover the most mundane items such as proper Demographics, proper labeling of the specimen, types of transport media, etc........, but while I was discussing this with a pathologist he mentioned it would be nice if the orders stated if the patient were pregnant or not. He was well aware he could look it up in the history, but felt it could save time if it were stated on the request. This got me to thinking and my thoughts turned to histonet. So if any of you would like to share some time saving protocols and procedures that I could review for the lecture please forward them to me and the NET. The title of the lecture will be "It's All UP Front". I only include the title in hopes it will jog a few brain cells and you'll think of other areas that might contribute to the histology department being able to improve patient care if everything is done right in the beginning. I would be very interested in knowing how you anticipate or should I say plan for the worst case scenario, since we usually have very small samples when it comes to needle biopsies. I am looking for ways that maxamizes the sample for not only testing today, but also testing in the future. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients named above. If you are not the intended recipient, you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. As required by federal and state laws, you need to hold this information as privileged and confidential. If you have received this communication in error, please notify the sender and destroy all copies of this communication and any attachments. From GauchV <@t> mail.amc.edu Mon Aug 28 11:43:04 2006 From: GauchV <@t> mail.amc.edu (Vicki Gauch) Date: Mon Aug 28 11:45:28 2006 Subject: [Histonet] Frozens section cutinng by resident Dr Message-ID: The techs routinely cut,stain and mount frozen sections at our institution but if frozens occur after hours the resident will do them if they are still on site otherwise the pathologist will do them . Vicki Vicki Gauch AMCH Albany, NY >>> "Richard Cartun" 8/25/2006 11:19:54 AM >>> Yes, we do it all the time. Richard Richard W. Cartun, Ph.D. Director, Immunopathology & Histology Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 (860) 545-0174 Fax >>> "Tahseen" 08/24/06 9:26 PM >>> Hi every body Are there any other hospitals out there where the pathologists or resident cut frozen section stain and mount their own cases? Muhammad Tahseen _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ----------------------------------------- CONFIDENTIALITY NOTICE: This email and any attachments may contain confidential information that is protected by law and is for the sole use of the individuals or entities to which it is addressed. If you are not the intended recipient, please notify the sender by replying to this email and destroying all copies of the communication and attachments. Further use, disclosure, copying, distribution of, or reliance upon the contents of this email and attachments is strictly prohibited. To contact Albany Medical Center, or for a copy of our privacy practices, please visit us on the Internet at www.amc.edu. From hfedor <@t> jhmi.edu Mon Aug 28 12:24:57 2006 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Mon Aug 28 12:25:22 2006 Subject: [Histonet] TMA Book In-Reply-To: <127D612DBCD77546A12C4746F7491C7F111AD1@lewis.afip.osd.mil> References: <127D612DBCD77546A12C4746F7491C7F111AD1@lewis.afip.osd.mil> Message-ID: <44F2EEA9.61A1.0088.3@jhmi.edu> Dear Ginny, if you read through the Beecher manual it has quite a bit of information about making TMA's using the Beecher instrument, In addition, http://tmalab.jhmi.edu/abouttma.htm You can go to our website and there is quite a bit of information avaialble that you can print out and read. TMA Handout - Detailed description of TMA uses, validation, design, construction, data handling, and image handling. From the USCAP Meeting, Washington D.C., 2003 National Society For Histotechnology Talk http://www.tmaj.com/files/pub/DeMarzo_Fedor_NSH_for_Oct_2003.ppt Tissue MicroArrays Short Course http://www.tmaj.com/files/pub/Short_Course_Tmas_2004.ppt good luck. Feel free to call if you have specific questions. Helen >>> "Achstetter, Virginia A." 8/25/2006 6:50 AM >>> Are there any good books out on how to prepare a tma block, trouble shooting, cutting techniques etc. Ginny Achstetter _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Helen L. Fedor B.S. Johns Hopkins University Pathology Department 600 N Wolfe St Marburg Room 406 Baltimore MD 21287 email: hfedor@jhmi.edu Phone: 410 614-1660 Pager: 410 283-3419 WARNING: E-mail sent over the Internet is not secure. Information sent by e-mail may not remain confidential. DISCLAIMER: This e-mail is intended only for the individual to whom it is addressed. It may be used only in accordance with applicable laws. If you received this e-mail by mistake, notify the sender and destroy the e-mail. From hfedor <@t> jhmi.edu Mon Aug 28 12:24:57 2006 From: hfedor <@t> jhmi.edu (Helen Fedor) Date: Mon Aug 28 12:25:24 2006 Subject: [Histonet] TMA Book In-Reply-To: <127D612DBCD77546A12C4746F7491C7F111AD1@lewis.afip.osd.mil> References: <127D612DBCD77546A12C4746F7491C7F111AD1@lewis.afip.osd.mil> Message-ID: <44F2EEA9.61A1.0088.3@jhmi.edu> Dear Ginny, if you read through the Beecher manual it has quite a bit of information about making TMA's using the Beecher instrument, In addition, http://tmalab.jhmi.edu/abouttma.htm You can go to our website and there is quite a bit of information avaialble that you can print out and read. TMA Handout - Detailed description of TMA uses, validation, design, construction, data handling, and image handling. From the USCAP Meeting, Washington D.C., 2003 National Society For Histotechnology Talk http://www.tmaj.com/files/pub/DeMarzo_Fedor_NSH_for_Oct_2003.ppt Tissue MicroArrays Short Course http://www.tmaj.com/files/pub/Short_Course_Tmas_2004.ppt good luck. Feel free to call if you have specific questions. Helen >>> "Achstetter, Virginia A." 8/25/2006 6:50 AM >>> Are there any good books out on how to prepare a tma block, trouble shooting, cutting techniques etc. Ginny Achstetter _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Helen L. Fedor B.S. Johns Hopkins University Pathology Department 600 N Wolfe St Marburg Room 406 Baltimore MD 21287 email: hfedor@jhmi.edu Phone: 410 614-1660 Pager: 410 283-3419 WARNING: E-mail sent over the Internet is not secure. Information sent by e-mail may not remain confidential. DISCLAIMER: This e-mail is intended only for the individual to whom it is addressed. It may be used only in accordance with applicable laws. If you received this e-mail by mistake, notify the sender and destroy the e-mail. From Marirose.Satterfield <@t> MercyMemorial.org Mon Aug 28 12:43:21 2006 From: Marirose.Satterfield <@t> MercyMemorial.org (Satterfield, Marirose) Date: Mon Aug 28 12:43:29 2006 Subject: [Histonet] Clinical Studies Message-ID: Our hospital has started to enroll patients in clinical studies. Most studies are strongly suggesting sending a tissue block rather than sending them unstained slides. I was wondering how other hospitals are handling this issue. M Satterfield From LINDA.MARGRAF <@t> childrens.com Mon Aug 28 12:46:22 2006 From: LINDA.MARGRAF <@t> childrens.com (LINDA MARGRAF) Date: Mon Aug 28 12:46:38 2006 Subject: [Histonet] Fwd: Determination of collagen content in rat myocardial Message-ID: Histonetters: Here is an inquiry (see below) I received which must be meant for the Histonet list . Please send your responses directly to Cristian at chopan24ac@gmail.com Thanks Linda M Histonet administrator >>> "cris aguilar" 08/22/06 12:29 PM >>> I am a medical student in Peru. At present, I have been developing my thesis in ventricular remodeling; therefore I need to quantify *"collagen content" in rat heart*, as fibrosis index. I have tried to quantify "collagen content by hydroxyproline determination" several times in my laboratory, but I failed. I have been following Reddy's technique: * * *A simplified method for analysis of Hydroxiproline in Biological tissues. * Reddy GK, Enwemeka CS. Clinical Biochemistry 1996;29:225-9. *My procedure: * * * *Preparation of myocardial samples: * The rats were weighed, anesthetized with sodium pentobarbital (50 mg/kg, intraperitoneal), and underwent median thoracotomy for heart removal. The left ventricle was separated and weighed. Later, the myocardial samples were "dehydrated" in pure alcohol for 24 hours (is this correct?). Finally, 50mg of wet tissue sample was manually homogenized (manualmente en un mortero) in 1mL NaCl. ** * * * Quantification of hydroxyproline* Duplicated Hyp standard (0, 2.0, 4.0, 6.0, 8.0, 10.0ml) and triplicated test samples (homogenate 10ml, 50mg wet tissue/ 1ml NaCl) were placed in high temperature polypropylene tubes of 2ml capacity. Later 50ml of 2N sodium hydroxide was added to each tube with Hyp standard and test samples and mixed at room temperature for 20 minutes. Samples were hydrolyzed (alkali hydrolysis in 2N NaOH) by fire (estufa) at 120?C for 20min. 50ml of Chloramine-T was added and mixed with the hydrolyzate and oxidation was allowed to proceed for 25 minutes at room temperature. 500ml of 1M Ehrlich's reagent was added to each sample and the samples were mixed and incubated at 65?C for 20 minutes. Finally the absorbance of samples was read at 550nm using a spectrophotometer. *1. **Is it necessary to dehydrate the sample for a later analysis? * * * *2. **Is alkaline hydrolysis (2N NaOH) the better way to liberate Hyp from samples? * * * *3. **Did the method use autoclave to hydrolyzate? I have been use a fire (estufa)?* *Is this correct?* * * *4. **How can I estimate Hydroxyproline content in rat heart (mg/g)?* * * Does anybody know how I can measure collagen content in rat heart? Has anyone done a Sirius red stain for collagen? If so, could I please have your protocol? *Help me please I am desperate, because I must present my thesis next October. * Thank you in advance. Cristian Aguilar Universidad Nacional de Trujillo From NMHisto <@t> aol.com Sat Aug 26 12:23:09 2006 From: NMHisto <@t> aol.com (NMHisto@aol.com) Date: Mon Aug 28 13:00:15 2006 Subject: [Histonet] BASEBALL, HOTDOGS & MISSING BRAIN CELLS Message-ID: <3b8.8383af2.3221dd7d@aol.com> To all those of you who have contacted me about going to a baseball game during NSH in Phoenix, I am sorry to say that my efforts to find a group of seats is proving to be more difficult than expected. It will be hard to coordinate available seats, scheduling with seminars and events at NSH for everyone (which are not known to me at this time), and a game that fits into those schedules. The sincerity of the Plan was there but it does not appear that this is something that can be accomplished. My motto is "I love it when a Plan comes together"..so I apologize that I was not able to pull this off! Sally Breeden From lblazek <@t> digestivespecialists.com Mon Aug 28 13:31:46 2006 From: lblazek <@t> digestivespecialists.com (Blazek, Linda) Date: Mon Aug 28 13:26:33 2006 Subject: [Histonet] BASEBALL, HOTDOGS & MISSING BRAIN CELLS Message-ID: <6CBA6DC98A079D408C87250591D9DFB802360DCB@bruexchange.digestivespecialists.com> Sally, It was a great thought though! What I want to know though, is if you snatched the seat behind home plate. Linda Blazek HT (ASCP) Manager/Supervisor GI Pathology of Dayton 7415 Brandt Pike Huber Heights, OH 45424 Phone: (937) 293-4424 ext 7118 Email: lblazek@digestivespecialists.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of NMHisto@aol.com Sent: Saturday, August 26, 2006 1:23 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BASEBALL, HOTDOGS & MISSING BRAIN CELLS To all those of you who have contacted me about going to a baseball game during NSH in Phoenix, I am sorry to say that my efforts to find a group of seats is proving to be more difficult than expected. It will be hard to coordinate available seats, scheduling with seminars and events at NSH for everyone (which are not known to me at this time), and a game that fits into those schedules. The sincerity of the Plan was there but it does not appear that this is something that can be accomplished. My motto is "I love it when a Plan comes together"..so I apologize that I was not able to pull this off! Sally Breeden _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From HornHV <@t> archildrens.org Mon Aug 28 14:23:04 2006 From: HornHV <@t> archildrens.org (Horn, Hazel V) Date: Mon Aug 28 14:23:22 2006 Subject: [Histonet] Clinical Studies In-Reply-To: Message-ID: <9AE8AA9E1F644B4AA6C155FB6FD51C630ACB6D73@EMAIL.archildrens.org> We usually cut unstained slides instead of sending blocks. We have sent blocks before and they will send them back if you ask for them. Hazel Horn Hazel Horn, HT/HTL (ASCP) Supervisor of Histology Arkansas Children's Hospital 800 Marshall Slot 820 Little Rock, AR 72202 phone 501.364.4240 fax 501.364.3912 visit us on the web at: www.archildrens.org -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Satterfield, Marirose Sent: Monday, August 28, 2006 12:43 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Clinical Studies Our hospital has started to enroll patients in clinical studies. Most studies are strongly suggesting sending a tissue block rather than sending them unstained slides. I was wondering how other hospitals are handling this issue. M Satterfield _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------------------------ The information contained in this message may be privileged and confidential and protected from disclosure. If the reader of this message is not the intended recipient, or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify us immediately by replying to the message and deleting it from your computer. Thank you. ============================================================================== From hdjajadi2 <@t> yahoo.com Mon Aug 28 15:56:19 2006 From: hdjajadi2 <@t> yahoo.com (Hidayat Djajadi) Date: Mon Aug 28 15:56:24 2006 Subject: [Histonet] General Staining for Protein, Lipid and Carbohydrate for Mice Eye Paraffin Section. Message-ID: <20060828205619.32799.qmail@web36306.mail.mud.yahoo.com> Dear All, My PI was looking for a general staining that easy to make for detecting a protein, lipid and carbohydrate on WT and Knock Out mice lens that fixed in Carnoys. This lens has vacuole that we would to stain. Thank you so much in advance for all your help guys. Regards, Dayat --------------------------------- Yahoo! Messenger with Voice. Make PC-to-Phone Calls to the US (and 30+ countries) for 2?/min or less. From lrichey <@t> u.washington.edu Mon Aug 28 17:19:44 2006 From: lrichey <@t> u.washington.edu (Lori Richey) Date: Mon Aug 28 17:19:54 2006 Subject: [Histonet] products suggestions for snap freezing In-Reply-To: References: Message-ID: <44F36C00.20702@u.washington.edu> I've only seen snap freezing done using liquid nitrogen. The isopentane can be stored in a nalgene bottle, and a nalgene cup can be used. Pour the liquid nitrogen into a wide mouth plastic cup, and lower slowly into the liquid Nitrogen. When white plaques start forming on the bottom of the cup, it is cold enough to snap freeze the tissue in a cryo mold. From Inga.Hansson <@t> neuro.uu.se Tue Aug 29 01:55:50 2006 From: Inga.Hansson <@t> neuro.uu.se (Inga Hansson) Date: Tue Aug 29 01:53:55 2006 Subject: [Histonet] Genway Biotech Message-ID: Hi histonetters in the US! Is anyone familiar with this company? I tried ordering an antibody from them some months ago through their webpage but I didn?t get any reply! They?re supposed to be located in San Diego. Thanks! Inga From vanann702 <@t> skmc.gov.ae Tue Aug 29 05:58:36 2006 From: vanann702 <@t> skmc.gov.ae (Anne Van Binsbergen) Date: Tue Aug 29 05:57:46 2006 Subject: [Histonet] cerner milennium Message-ID: Hello histonetters Cerner Millenium is the HIS/LIS of choice here in Abu Dhabi. There are 5 hospitals in the project and we have all been working really hard for almost a year - things move slowly here in the desert heat - plans are to 'go live' sometime early next year. I have questions which I believe could only really be answered by someone in my position (AP PATHNET application specialist-in-training) who has 'gone live' and survived a year beyond that, using the same system. My plea is for that 'someone' to contact me directly so that I can try to get some answers to the many questions which are always 'beyond the scope' of Cerner. Yours hopefully Annie From sheila_adey <@t> hotmail.com Tue Aug 29 06:18:38 2006 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Tue Aug 29 06:18:45 2006 Subject: [Histonet] co path In-Reply-To: Message-ID: We also use Copath but I'm not aware how to pull a separate log for specials. I don't work on the LIS end of the system. Which leads me to my question, does anyone who uses copath know how to just get the slide label that you need? When we order levels the entire case reprints. It is such a waste of labels. Thanks Sheila Adey HT, MLT Port Huron Hospital >From: "Jim Ball" >To: histonet@lists.utsouthwestern.edu >Subject: [Histonet] co path >Date: Mon, 21 Aug 2006 07:44:54 -0400 > >The Facility I am presently working with has the Co path + operating >system. I am total ignorant of the system, but I am sure there must be a >procedure for sorting out special stains. The Techs are presently going >through the entire Log and listing the special stains manually. The entire >procedure can take up ward to 45 minutes for an experienced Tech and much >longer for a first timer. Hopefully some one out there can suggest a >procedure for pulling out only the special stains on to their own log, and >hopefully a means of logging each special stain on their own log so >separate work sheets could become a thing of the past. > > The path department has already approached the LIS department, but >there seems to be some sort of language barrier present Java vs simple >English. > > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Back to school shopping is as easy as 1-2-3 http://shopping.sympatico.msn.ca/content/shp/?ctId=493,ptnrid=176,ptnrdata=081803 From Kari.Zajic <@t> HCAhealthcare.com Tue Aug 29 07:55:07 2006 From: Kari.Zajic <@t> HCAhealthcare.com (Zajic Kari) Date: Tue Aug 29 07:55:12 2006 Subject: [Histonet] Frozen section case "charges" Message-ID: <095327C7CDBDF64B9E9728A54799091E015CC626@ORLEV03.hca.corpad.net> Hello all Histonetters! I have been asked by a pathologist to do occasional skin frozen section cases and was wondering what a fair "charge" for my services would be. I do not have to travel nor process the cases, just assist and cut the frozen section. Should I be charging per block/frozen or per case? They only take approximately 20-30 minutes to do according to the size of the specimen. I would appreciate the feedback, especially from my fellow techs in Florida... Thank you! Kari From denise.woodward <@t> uconn.edu Tue Aug 29 09:40:21 2006 From: denise.woodward <@t> uconn.edu (Woodward, Denise) Date: Tue Aug 29 09:40:32 2006 Subject: [Histonet] Wearing gloves while cutting sections- One more response Message-ID: A little late but......... another option is to have your "task analysis" for paraffin microtomy in your OSHA-required lab safety plan state which PPE is necessary for the task. Is Maureen Doran out there to confirm?? Thanks, Denise Long Woodward UConn Dept. of Pathobiology & Vertinary Sciences CMVDL Storrs, CT -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito Sent: Friday, August 25, 2006 9:26 PM To: Janice Mahoney; histonet@lists.utsouthwestern.edu; RBARNHART@summithealth.org Subject: Re: [Histonet] Wearing gloves while cutting sections thank you. Enjoy the weekend ----- Original Message ----- From: "Janice Mahoney" To: ; Sent: Friday, August 25, 2006 6:52 AM Subject: Re: [Histonet] Wearing gloves while cutting sections I've been in this field for over 30 years and have never heard of Hank solution. Do I want to drink it? And Joe, if anyone out there in Histoland ever questioned whether or not you are a nut, they now know. >>> "Rebecca Barnhart" 08/25/2006 6:45 AM >>> Wow! I thought it was weird when I once witnessed a pathologist drink Hank solution to determine if it was similar to a drink. Of course the Hank was fresh. That experience explained a lot about that pathologist. >>> "Joe Nocito" 8/24/2006 7:42:04 PM >>> Lick the block!? Damn, I thought I was the only one doing that. I used to do that with Pap smears. "This one is negative, negative, negative, positive, negative, oooh, this has HPV". Y'all didn't think I'd let this go by without a comment, did you? I love Fridays!!! Ooops, it's only Thursday. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Rene J Buesa" To: "LeVier, Rebecca J" ; "Scott, Allison D" ; Sent: Thursday, August 24, 2006 3:21 PM Subject: RE: [Histonet] Wearing gloves while cutting sections > Rebecca: > I think that you should expalin your thoughtful and illustrious commettee > that once the tissue is fixed and processed (unless it is KJ disease case) > you can lick the block. > By the way, I once did just that in front of the safety officer of our > lab (she left me alone for ever. I still don't know if she left me alone > because she was convinced or because she thought that I was completely out > of my mind; it worked!). > Ren? J. > > "LeVier, Rebecca J" wrote: > I am not sure that the committee fully understood what the process was. > Could they have been looking at the wrong thing. Did they really mean to > site for this or were they just having a misunderstanding? If it were > me, I would try to get together with them to talk. For me, it is almost > impossible for me to grab a ribbon with gloves on. > > -----Original Message----- > From: histonet-bounces@lists.utsouthwestern.edu > [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott, > Allison D > Sent: Thursday, August 24, 2006 3:07 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Wearing gloves while cutting sections > > While I was on vaction, the safety committee came around and did rounds > in the lab. They sited us for not wearing gloves while cutting > sections. My techs tried to explain to them that we did not have to > wear gloves, only when cutting frozen sections or while handling fresh > tissue. I have never worn gloves while cutting paraffin sections. Now > my manager wants me to write a policy in regards to not wearing gloves. > Does anyone have anything like this in place that they would be willing > to share with me. Your help would be greatly appreciated. > > Allison Scott HT(ASCP) > Histology Supervisor > LBJ Hospital > Houston, Texas 77026 > > CONFIDENTIALITY NOTICE: > If you have received this e-mail in error, please immediately notify the > sender by return e-mail and delete this e-mail and any attachments from > your computer system. > > To the extent the information in this e-mail and any attachments contain > protected health information as defined by the Health Insurance > Portability and Accountability Act of 1996 ("HIPAA"), PL 104-191; 45 CFR > Parts 160 and 164; or Chapter 181, Texas Health and Safety Code, it is > confidential and/or privileged. This e-mail may also be confidential > and/or privileged under Texas law. The e-mail is for the use of only > the individual or entity named above. If you are not the intended > recipient, or any authorized representative of the intended recipient, > you are hereby notified that any review, dissemination or copying of > this e-mail and its attachments is strictly prohibited. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > Confidentiality Notice: This e-mail message, including any > attachments, is for the sole use of intended recipient(s) and may > contain confidential and privileged information. Any unauthorized > review, use, disclosure or distribution is prohibited. If you are not > the intended recipient, please contact the sender by reply e-mail and > destroy all copies of the original message. > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > --------------------------------- > Get your own web address for just $1.99/1st yr. We'll help. Yahoo! Small > Business. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > No virus found in this incoming message. > Checked by AVG Free Edition. > Version: 7.1.405 / Virus Database: 268.11.5/426 - Release Date: 8/23/2006 > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.405 / Virus Database: 268.11.6/427 - Release Date: 8/24/2006 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Stacy_McLaughlin <@t> cooley-dickinson.org Tue Aug 29 10:51:16 2006 From: Stacy_McLaughlin <@t> cooley-dickinson.org (Stacy McLaughlin) Date: Tue Aug 29 10:51:28 2006 Subject: [Histonet] Cell blocks Message-ID: Hello In Histoland, Our pathologists suddenly decided that they were unhappy with the way cell blocks were made here. (I'm asking for our Cyto Dept.) Would anyone be willing to share their procedure? Thanks in advance, Stacy McLaughlin Cooley Dickinson Hospital From tpmorken <@t> labvision.com Tue Aug 29 11:04:53 2006 From: tpmorken <@t> labvision.com (Morken, Tim) Date: Tue Aug 29 11:05:08 2006 Subject: [Histonet] Cell blocks Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA2D94D62@usca0082k08.labvision.apogent.com> Stacy, How are the cell blocks made now? Tim Morken -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Stacy McLaughlin Sent: Tuesday, August 29, 2006 8:51 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Cell blocks Hello In Histoland, Our pathologists suddenly decided that they were unhappy with the way cell blocks were made here. (I'm asking for our Cyto Dept.) Would anyone be willing to share their procedure? Thanks in advance, Stacy McLaughlin Cooley Dickinson Hospital From dassog <@t> evergreen.edu Tue Aug 29 11:19:56 2006 From: dassog <@t> evergreen.edu (Dasso, Greg (staff)) Date: Tue Aug 29 11:20:03 2006 Subject: [Histonet] Mounting large cryosections Message-ID: <3872E8431D06E545AC955BED177CB6F601604926@oak.evergreen.edu> Histonetters, I've been preparing CryoJane slides for my cryo sections on 25mm slides for the last year and they work great for 1 square-inch blocks. Our research has advanced and I am moving to a 4 square-inch machine that requires a 48mm slide. CryoJane makes their 2-inch tape for section collection, however they do not make a 48mm slide with the adhesive coating used on their 24mm slides. I had heard that albumin will work, but was wondering if anyone else is mounting larger, whole-animal cryo sections and what solutions you could offer. thanks in advance, Greg Dasso Barlow Scientific, Inc dassog@evergreen.edu From pkarlisch <@t> psu.edu Tue Aug 29 11:32:53 2006 From: pkarlisch <@t> psu.edu (Patricia Karlisch) Date: Tue Aug 29 11:33:08 2006 Subject: [Histonet] Small endoscopy specimens Message-ID: <44F433F50200008C00020AE1@GWIA02.HERSHEYMED.NET> All, We are having occassional problems with small colon, stomach and antrum specimens that are 'drying' out, at least this is what we think. The specimens show no nuclear detail and the H&E looks smudgy. Other specimens stained with the same slides look great. We process our small specimens on a short 5 hour processing schedule which seemed to have improved some of our 85% of our specimens. Has anyone else had this problem? We think that the specimen may be sitting in the OR before they get placed in formalin. Is there any way to fix this when it happens? Any suggestions would be helpful. Pat Karlisch Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. From rjbuesa <@t> yahoo.com Tue Aug 29 11:41:04 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Aug 29 11:41:10 2006 Subject: [Histonet] Cell blocks In-Reply-To: Message-ID: <20060829164104.89917.qmail@web61217.mail.yahoo.com> Stacy: Excuse me but before going to all the trouble of changing a procedure, why don't you ask your PT what is the problem. Are THEY not able to diagnose? Are the blocks all of a sudden not providing the information they neEd? Usually when that type of a request surfaces is because somebody tells them how they do their cell blocks and they decide (subjectively) that the new way is better. In that case they have a "NEW" procedure. Ask them: How do you want me to process the cell blocks, and let them come with a logical answer. There are not that many ways of doing a good cell block, by the way! Just my opinion! (I never used to bend backwards at the first attempt!) Ren? J. Stacy McLaughlin wrote: Hello In Histoland, Our pathologists suddenly decided that they were unhappy with the way cell blocks were made here. (I'm asking for our Cyto Dept.) Would anyone be willing to share their procedure? Thanks in advance, Stacy McLaughlin Cooley Dickinson Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- All-new Yahoo! Mail - Fire up a more powerful email and get things done faster. From rjbuesa <@t> yahoo.com Tue Aug 29 11:44:01 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Aug 29 11:44:09 2006 Subject: [Histonet] Mounting large cryosections In-Reply-To: <3872E8431D06E545AC955BED177CB6F601604926@oak.evergreen.edu> Message-ID: <20060829164401.10633.qmail@web61224.mail.yahoo.com> Greg: Depending on what you are going to use the sections afterwards, Mayer's albumen is good (if dried on the selides before adding the cryosection). If you are going to use them for IHC, Mayer's will give you too much background. Ren? J. "Dasso, Greg (staff)" wrote: Histonetters, I've been preparing CryoJane slides for my cryo sections on 25mm slides for the last year and they work great for 1 square-inch blocks. Our research has advanced and I am moving to a 4 square-inch machine that requires a 48mm slide. CryoJane makes their 2-inch tape for section collection, however they do not make a 48mm slide with the adhesive coating used on their 24mm slides. I had heard that albumin will work, but was wondering if anyone else is mounting larger, whole-animal cryo sections and what solutions you could offer. thanks in advance, Greg Dasso Barlow Scientific, Inc dassog@evergreen.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. From rjbuesa <@t> yahoo.com Tue Aug 29 11:53:12 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Tue Aug 29 11:53:15 2006 Subject: [Histonet] Small endoscopy specimens In-Reply-To: <44F433F50200008C00020AE1@GWIA02.HERSHEYMED.NET> Message-ID: <20060829165312.83183.qmail@web61211.mail.yahoo.com> Patricia: You yourself have pointed out to the 2 main sources of the problem: 1- a longer than needed processing schedule is one. Reducing it, specially the dehydrating stations, can help, and 2- doing a better coordination between the OR and the lab (with regards to how expeditiously the Bx are fixed/delivered). In our case we did the same two things but we really eliminated the problem when we eliminated xylene in our tissue processing and changed to mineral oil. If you want I can send you that protocol. Regards Ren? J. Patricia Karlisch wrote: All, We are having occassional problems with small colon, stomach and antrum specimens that are 'drying' out, at least this is what we think. The specimens show no nuclear detail and the H&E looks smudgy. Other specimens stained with the same slides look great. We process our small specimens on a short 5 hour processing schedule which seemed to have improved some of our 85% of our specimens. Has anyone else had this problem? We think that the specimen may be sitting in the OR before they get placed in formalin. Is there any way to fix this when it happens? Any suggestions would be helpful. Pat Karlisch Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Stay in the know. Pulse on the new Yahoo.com. Check it out. From liz <@t> premierlab.com Tue Aug 29 12:28:07 2006 From: liz <@t> premierlab.com (Liz Chlipala) Date: Tue Aug 29 12:23:23 2006 Subject: [Histonet] hyaluronidase digestion for aggrecan staining Message-ID: <000001c6cb90$7f8639b0$0300a8c0@domain.Premier> Hello All We have been working on aggrecan immunostain on articular cartiage and wanted to perform a hyaluronidase digestion prior. I have searched extensively and have come up with few methods, none of which is complete. Does anyone out there have a method that includes the molarity of the buffer, the pH of the buffer and the amount of hyaluronidase used and the length of time and temp, etc.. Any advice would be helpful. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 From hborgeri <@t> wfubmc.edu Tue Aug 29 12:56:22 2006 From: hborgeri <@t> wfubmc.edu (Hermina Borgerink) Date: Tue Aug 29 12:56:37 2006 Subject: [Histonet] hyaluronidase digestion for aggrecan staining Message-ID: <9AEEF1FB6254224AA355ED285F8491651B332A52@EXCHVS2.medctr.ad.wfubmc.edu> Liz, Many years ago when I was still routinely doing immuno staining on bone and articular cartilage, this is the formula for hyaluronidase digestion I used: Hyaluronidase 0.1mg/ml bovine testicular hyaluronidase 0.05M Tris-HCl pH 7.6 0.15M NaCl 0.1% CaCl2 deionized water Incubate sections at room temperature for 30 minutes prior to primary antibody incubation. Hermina -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Tuesday, August 29, 2006 1:28 PM To: 'Histonet' Subject: [Histonet] hyaluronidase digestion for aggrecan staining Hello All We have been working on aggrecan immunostain on articular cartiage and wanted to perform a hyaluronidase digestion prior. I have searched extensively and have come up with few methods, none of which is complete. Does anyone out there have a method that includes the molarity of the buffer, the pH of the buffer and the amount of hyaluronidase used and the length of time and temp, etc.. Any advice would be helpful. Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 735-5001 fax (303) 735-3540 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC University of Colorado at Boulder MCDB, Room A3B40 Boulder, CO 80309 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From portera <@t> msu.edu Tue Aug 29 13:32:11 2006 From: portera <@t> msu.edu (Amy Porter) Date: Tue Aug 29 13:31:12 2006 Subject: [Histonet] Cytopathology Text Message-ID: <002201c6cb99$70e92440$8e7a0923@HistoJJ> I am looking for a good reference text for cytopathology with clincal images. Our pathologist just received information to purchase Silverberg's Principles and Practice of Surgical Pathology and Cytopathology. Since our laboratory does only research I was curious as to whether there would be a better text for strictyl Cytopathology out there, and if anyone has Siverberg's in their facility as a histotech how do you feel about the cytology content. Thanks in advance - Amy S. Porter, HT(ASCP) QIHC - Supervisor Michigan State University Investigative HistoPathology Laboratory Department of Physiology / Division of Human Pathology 2100 Biomedical Physical Sciences Bldg. Room #2133 East Lansing, MI 48824-3320 Phone: (517) 355-6475 ext 1480 / Fax: (517) 432-1368 Email: portera@msu.edu www.humanpathology.msu.edu From emineo <@t> mccormickscientific.com Tue Aug 29 13:48:16 2006 From: emineo <@t> mccormickscientific.com (Emmanuel Mineo) Date: Tue Aug 29 13:48:00 2006 Subject: [Histonet] Mounting large cryosections Message-ID: <5784D843593D874C93E9BADCB87342AB01E58167@tpiserver03.Coretech-holdings.com> Greg, Instrumedics produces a Macro Tape-Transfer system that will allow you to obtain sections as large as 5x7 inches. If you would like more information on the system please feel free to contact me. Cordially, Emmanuel I. Mineo Product Manager Instrumedics, Inc. 5918 Evergreen Boulevard St. Louis, MO 63134 Email: emineo@instrumedics.com Phone: (314) 522-8671 Fax: (314) 522-6360 Toll Free: (800)729-4421 Cell: 973-255-8326 www.instrumedics.com www.mccormickscientific.com www.vibratome.com www.myneurolab.com www.theglassworx.com -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Dasso, Greg (staff) Sent: Tuesday, August 29, 2006 10:20 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mounting large cryosections Histonetters, I've been preparing CryoJane slides for my cryo sections on 25mm slides for the last year and they work great for 1 square-inch blocks. Our research has advanced and I am moving to a 4 square-inch machine that requires a 48mm slide. CryoJane makes their 2-inch tape for section collection, however they do not make a 48mm slide with the adhesive coating used on their 24mm slides. I had heard that albumin will work, but was wondering if anyone else is mounting larger, whole-animal cryo sections and what solutions you could offer. thanks in advance, Greg Dasso Barlow Scientific, Inc dassog@evergreen.edu _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet *** Confidentiality Statement *** This e-mail is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged and confidential. If the reader of this message is not the intended recipient, please notify the sender immediately by replying to this message and then delete it from your system. Any review, dissemination, distribution, or reproduction of this message by unintended recipients is strictly prohibited and may be subject to legal restriction. Thank you for your cooperation. From bayoubelle311 <@t> gmail.com Tue Aug 29 13:57:22 2006 From: bayoubelle311 <@t> gmail.com (Missy) Date: Tue Aug 29 13:57:27 2006 Subject: [Histonet] rat femur histopath Message-ID: <398f02c20608291157k5798c130oeea498d0db55cd62@mail.gmail.com> Hi all, I am looking for a procedure for delcalcifying rat femur. I want to visualize and quantify BM fibrosis using Gomori stain. If anyone has a good protocol please let me know. Thank you Melissa Aycock bayoubelle311@gmail.com From themagoos <@t> rushmore.com Tue Aug 29 13:57:47 2006 From: themagoos <@t> rushmore.com (Jason M. McGough) Date: Tue Aug 29 13:58:14 2006 Subject: [Histonet] Iron Controls References: <002201c6cb99$70e92440$8e7a0923@HistoJJ> Message-ID: <003e01c6cb9d$057b2ba0$102c9e43@magoo> I was wondering if anybody has a good iron control. I would be willing to trade an AFB control for an iron control. Thanks, Jason McGough, HT(ASCP) Account Representative-Anatomic Pathology Clinical Laboratory of the Black Hills 2805 5th St. Suite 210 Rapid City, SD 57701 605-343-2267 jmcgough@clinlab.com From gcallis <@t> montana.edu Tue Aug 29 14:29:12 2006 From: gcallis <@t> montana.edu (Gayle Callis) Date: Tue Aug 29 14:29:17 2006 Subject: [Histonet] rat femur histopath In-Reply-To: <398f02c20608291157k5798c130oeea498d0db55cd62@mail.gmail.co m> References: <398f02c20608291157k5798c130oeea498d0db55cd62@mail.gmail.com> Message-ID: <6.0.0.22.1.20060829132306.01b57e88@gemini.msu.montana.edu> Missy, Any acid decalcifier should work fine, we use 10% formic acid but you need to do endpoint determinations to make sure the bone and soft tissues are not ruined by the effects of overexposure to acid aka overdecalcification. Rinse the bones for a few hours, process longer into paraffin unless you are bisecting the femurs. We did our rat femurs for 2 hours per station on a VIP, and use Tissue Prep 2, a harder paraffin that is better for sectioning for rat femurs and whole rat knees. I have a quick weight loss/weight gain endpoint determination that works well if you do not have a FAXITRON to xray for endpoint, or do not want to do a chemical endpoint test - you can do that too. At 12:57 PM 8/29/2006, you wrote: >Hi all, > >I am looking for a procedure for delcalcifying rat femur. I want to >visualize and quantify BM fibrosis using Gomori stain. If anyone has a good >protocol please let me know. > >Thank you >Melissa Aycock >bayoubelle311@gmail.com >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet Gayle Callis MT,HT,HTL(ASCP) Research Histopathology Supervisor Veterinary Molecular Biology Montana State University - Bozeman PO Box 173610 Bozeman MT 59717-3610 406 994-6367 406 994-4303 (FAX) From jpmelendez <@t> peoplepc.com Tue Aug 29 14:40:16 2006 From: jpmelendez <@t> peoplepc.com (Darling JeanPaulMelendez) Date: Tue Aug 29 14:40:21 2006 Subject: [Histonet] Input for academic survey Message-ID: <9440589.1156880417058.JavaMail.root@mswamui-billy.atl.sa.earthlink.net> Greetings fellow laboratory scientist! According to R.D. Laing, "we live in a moment of history where change is so speeded up that we begin to see the present only when it is already disappearing". In relation to this project, through questioning, we gain insight to further the search for enlightenment into the impact of technological integration in the Histopathology field. Data gathered from this survey is used to gather research information for academic purposes. All responses will be kept confidential and anonymous. Please take a moment to click on this link: http://www.surveymonkey.com/s.asp?u=972542486468 to complete this survey. Upon completion of this survey, should anyone desire to express additional input on the impact of technological integration in their lab, please email me. Thank you, Darling Jean-Paul-Melendez ________________________________________ PeoplePC Online A better way to Internet http://www.peoplepc.com From tpmorken <@t> labvision.com Tue Aug 29 15:03:08 2006 From: tpmorken <@t> labvision.com (Morken, Tim) Date: Tue Aug 29 15:03:18 2006 Subject: [Histonet] Carrie K-B, I know you are out there! Message-ID: <0556BE8AC5551E4E8AF6BB9E42509BA2D94D71@usca0082k08.labvision.apogent.com> Give me a call sometime! Tim Morken Product Development Lab Vision - Neomarkers 47777 Warm Springs Blvd. Fremont, CA 94539 USA tel: 01-510-991-2840 fax: 01 510-991-2826 email: tpmorken@labvision.com web: www.labvision.com From tncperfect2 <@t> comcast.net Tue Aug 29 18:02:02 2006 From: tncperfect2 <@t> comcast.net (tncperfect2@comcast.net) Date: Tue Aug 29 18:02:07 2006 Subject: [Histonet] Amount of time to cut slides Message-ID: <082920062302.29793.44F4C76A0005BFBE000074612207300793CD9B0C0A009D0A9F0C029B@comcast.net> Hello Histonetters, I have asked questions and you have always come through for me. I have one more for you. Is there a CAP survey or a study that is recognized by CAP that shows how much time it takes from grossing to having the slide ready for the pathologist? I could not find any information through the CAP website so I am coming to the people who know the most about this. Any help will be appreciated. Carolyn From JMacDonald <@t> mtsac.edu Tue Aug 29 23:29:57 2006 From: JMacDonald <@t> mtsac.edu (Jennifer MacDonald) Date: Tue Aug 29 23:30:20 2006 Subject: [Histonet] Iron Controls Message-ID: Jason, Spleen works well as an iron control. -----histonet-bounces@lists.utsouth To: "Histonet" < "Jason M. McGough" Sent by: hist Date: 08/29/2006 11:57AM Subjec I was wondering if anybody has a good iro willing to trade an AFB control for an iron contr Thanks, Jason McGough, HT(ASCP) Account Representative Clinical Laboratory of the Black Hills 2805 5th S Rapid City, SD 57701 605-343-2267 jmcgough@clinlab.co ___________________ ______________________ 5F__ Histonet mailing list Histonet@lists.utsouthwestern.edu References 1. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet" From xiao-qun.zhang <@t> imbim.uu.se Wed Aug 30 03:03:29 2006 From: xiao-qun.zhang <@t> imbim.uu.se (Xiao-Qun Zhang) Date: Wed Aug 30 03:02:15 2006 Subject: [Histonet] Ethanol fixation Message-ID: Hello All, I am new for the histonet. I am sorry If my question has already been discussed many times. My question is if E14.5 mouse embryos fixed over night in 4% PFA in the cold room and then kept in 70 % ethanol for 4 days in the cold room will still be ok for sectioning and in situ hybridization? Any comments are highly appreciated! Xiaoqun Zhang -- ---------------------------------------------------------------------------------------------- Xiao-Qun Zhang, PhD Dept. of Medical Biochemistry and Microbiology Box 582, Biomedical Center S-751 23 Uppsala, Sweden Phone: +46 (0)18 471 4260 Fax: +46 (0)18 471 42 44 Email: Xiao-Qun.Zhang@imbim.uu.se From dsnider <@t> shrinenet.org Wed Aug 30 06:47:45 2006 From: dsnider <@t> shrinenet.org (Snider, Deanna) Date: Wed Aug 30 06:47:45 2006 Subject: [Histonet] Technovitt 7100 GMA kit Message-ID: <84BE46B37B314D409C5A17B7BAB022D6B80E3B@IDC-EX-VS01.shriners.cc> Hello again, I hope some of you in histoland know more about this stuff than I do. I have been doing GMA's with this kit for the last 2 years and have never had a problem. I ordered a new kit and the first run I did, never cured. It was still very soft and unable to be cut. I did a second run, and since I live in an area of higher humidity I increased the hardener. (I actually doubled it;4mls/30mls.) It never cured either. I called the company and they replaced the kit. With the same lot #. I did to test runs with the same result! I noticed the cold pak they put in the top of the kit for shipping was no longer cold.(it is in the low 90's these days.) My question is this: How important is it that the chemicals in the kit stay cold? How are they affected by heat? The technical person at the company was pretty much useless, since he seemed to know less about the product than I do! Thanks in advance, Deanna Snider HT ASCP Lead Technician Shriners Hospital for Children Cincinnati, Oh 513-872-6388 CONFIDENTIALITY NOTICE: This e-mail communication and any attachments may contain confidential and privileged information for the use of the designated recipients. If you are not the intended recipient, (or authorized to receive for the recipient) you are hereby notified that you have received this communication in error and that any review, disclosure, dissemination, distribution or copying of it or its contents is prohibited. If you have received this communication in error, please destroy all copies of this communication and any attachments and contact the sender by reply e-mail or telephone (813) 281-0300. From joanna.c.barton <@t> GSK.COM Wed Aug 30 08:53:32 2006 From: joanna.c.barton <@t> GSK.COM (joanna.c.barton@GSK.COM) Date: Wed Aug 30 08:53:47 2006 Subject: [Histonet] Paraplast Plus Message-ID: Hi All, I wondered if anyone has had any problems recently with Paraplast Plus? We have had some problems with infiltration during processing, as well as some type of droplets in the paraffin after it has been melted. Thank you, Joanna From rjbuesa <@t> yahoo.com Wed Aug 30 09:00:48 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Aug 30 09:00:54 2006 Subject: [Histonet] Amount of time to cut slides In-Reply-To: <082920062302.29793.44F4C76A0005BFBE000074612207300793CD9B0C0A009D0A9F0C029B@comcast.net> Message-ID: <20060830140048.43260.qmail@web61219.mail.yahoo.com> Carolyn: In 2002 CAP published, as a result of a joint work with NSH, a short note titled:How many cassettes should be processed by a histotechnologist during an eight-hour shift? Appeared in CAP Today and you can get it at http://www.cap.org/apps/docs/cap_today/q_and_a/qa_1102.html The amount of work they recommended is unrealistically low as has been demonstrated by several works published later. Under separate cover I am sending a paper I published on Advance on this subject. Hope this will help you Ren? J. tncperfect2@comcast.net wrote: Hello Histonetters, I have asked questions and you have always come through for me. I have one more for you. Is there a CAP survey or a study that is recognized by CAP that shows how much time it takes from grossing to having the slide ready for the pathologist? I could not find any information through the CAP website so I am coming to the people who know the most about this. Any help will be appreciated. Carolyn _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Get on board. You're invited to try the new Yahoo! Mail Beta. From David.Muskett <@t> RLC.NHS.UK Wed Aug 30 09:44:00 2006 From: David.Muskett <@t> RLC.NHS.UK (Muskett David) Date: Wed Aug 30 09:44:07 2006 Subject: [Histonet] Meditech - billing function Message-ID: Hello I would like to hear from anyone who is using Meditech for the workload and billing functions in Histology. In the UK we haven't been very focused on billing in the past but I believe this will change over time. If anyone is willing to share any procedures or documents that would be even better. Regards David David Muskett Chief Biomedical Scientist, Histology Royal Liverpool Children's NHS Trust Eaton Road Liverpool L12 2AP Internal telephone x3615 Telephone (0151) 293 3656 Fax (0151) 293 3617 http://nhsald02/histopathology From histology <@t> gradymem.org Wed Aug 30 10:19:11 2006 From: histology <@t> gradymem.org (histology@gradymem.org) Date: Wed Aug 30 10:19:29 2006 Subject: [Histonet] SNOMED dx coding Message-ID: <929282928a66.928a66929282@onenet.net> Does anyone have experience with SNOMED coding? I need a code for "glandular stromal breakdown". We can't find this diagnosis in our SNOMED book. Could it be called somethng else? Thanks, Angie Barnett, HTL(ASCP) Grady Memorial Hospital Pathology Department 405/224-2258 histology@gradymem.org From sheila_adey <@t> hotmail.com Wed Aug 30 10:21:11 2006 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Wed Aug 30 10:21:27 2006 Subject: [Histonet] Small endoscopy specimens In-Reply-To: <20060829165312.83183.qmail@web61211.mail.yahoo.com> Message-ID: Hi, Do you use mineral oil on your processor for large specimens to? I would like to see your protocol aswell. We have on occasion had this same problem. Thanks Sheila HT, MLT Port Huron Hospital >From: Rene J Buesa >To: Patricia Karlisch , >histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] Small endoscopy specimens >Date: Tue, 29 Aug 2006 09:53:12 -0700 (PDT) > >Patricia: > You yourself have pointed out to the 2 main sources of the problem: > 1- a longer than needed processing schedule is one. Reducing it, >specially the dehydrating stations, can help, and > 2- doing a better coordination between the OR and the lab (with regards >to how expeditiously the Bx are fixed/delivered). > In our case we did the same two things but we really eliminated the >problem when we eliminated xylene in our tissue processing and changed to >mineral oil. > If you want I can send you that protocol. > Regards > René J. > >Patricia Karlisch wrote: > All, >We are having occassional problems with small colon, stomach and >antrum specimens that are 'drying' out, at least this is what we think. >The specimens show no nuclear detail and the H&E looks smudgy. Other >specimens stained with the same slides look great. We process our small >specimens on a short 5 hour processing schedule which seemed to have >improved some of our 85% of our specimens. Has anyone else had this >problem? We think that the specimen may be sitting in the OR before >they get placed in formalin. Is there any way to fix this when it >happens? Any suggestions would be helpful. Pat Karlisch > >Pat Karlisch >Supervisor, Histology, Pathology and Laboratory Medicine >Penn State Milton S. Hershey Medical Center >Mail Code H179 >Hershey, PA 17033 >Phone (717) 531-6072 >Fax: (717) 531- 7741 >email: pkarlisch@psu.edu > >*****E-Mail Confidentiality Notice***** >This message (including any attachments) contains information intended >for a specific individual(s) and purpose that may be privileged, >confidential or otherwise protected from disclosure pursuant to >applicable law. Any inappropriate use, distribution or copying of the >message is strictly prohibited and may subject you to criminal or civil >penalty. If you have received this transmission in error, please reply >to the sender indicating this error and delete the transmission from >your system immediately. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Stay in the know. Pulse on the new Yahoo.com. Check it out. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Back to school shopping is as easy as 1-2-3 http://shopping.sympatico.msn.ca/content/shp/?ctId=493,ptnrid=176,ptnrdata=081803 From sheila_adey <@t> hotmail.com Wed Aug 30 10:23:47 2006 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Wed Aug 30 10:23:54 2006 Subject: [Histonet] Cell blocks In-Reply-To: <20060829164104.89917.qmail@web61217.mail.yahoo.com> Message-ID: We use the shandon cell block fluid to form a clot with the cell button and pull it out on to the tissue paper. Works GREAT. No more loss of cells in the funnels. >From: Rene J Buesa >To: Stacy McLaughlin >,histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] Cell blocks >Date: Tue, 29 Aug 2006 09:41:04 -0700 (PDT) > >Stacy: > Excuse me but before going to all the trouble of changing a procedure, >why don't you ask your PT what is the problem. > Are THEY not able to diagnose? Are the blocks all of a sudden not >providing the information they neEd? > Usually when that type of a request surfaces is because somebody tells >them how they do their cell blocks and they decide (subjectively) that the >new way is better. > In that case they have a "NEW" procedure. > Ask them: How do you want me to process the cell blocks, and let them >come with a logical answer. > There are not that many ways of doing a good cell block, by the way! > Just my opinion! (I never used to bend backwards at the first attempt!) > René J. > >Stacy McLaughlin wrote: > Hello In Histoland, >Our pathologists suddenly decided that they were unhappy with the way cell >blocks were made here. (I'm asking for our Cyto Dept.) Would anyone be >willing to share their procedure? >Thanks in advance, >Stacy McLaughlin >Cooley Dickinson Hospital >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- > All-new Yahoo! Mail - Fire up a more powerful email and get things done >faster. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Back to school shopping is as easy as 1-2-3 http://shopping.sympatico.msn.ca/content/shp/?ctId=493,ptnrid=176,ptnrdata=081803 From rjbuesa <@t> yahoo.com Wed Aug 30 10:30:28 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Aug 30 10:30:32 2006 Subject: [Histonet] Small endoscopy specimens In-Reply-To: Message-ID: <20060830153028.67212.qmail@web61224.mail.yahoo.com> Sheila: Mineral oil is our standard procedure for ALL specimens. I am sending you the procedure under separate cover. Ren? J. sheila adey wrote: Hi, Do you use mineral oil on your processor for large specimens to? I would like to see your protocol aswell. We have on occasion had this same problem. Thanks Sheila HT, MLT Port Huron Hospital >From: Rene J Buesa >To: Patricia Karlisch , >histonet@lists.utsouthwestern.edu >Subject: Re: [Histonet] Small endoscopy specimens >Date: Tue, 29 Aug 2006 09:53:12 -0700 (PDT) > >Patricia: > You yourself have pointed out to the 2 main sources of the problem: > 1- a longer than needed processing schedule is one. Reducing it, >specially the dehydrating stations, can help, and > 2- doing a better coordination between the OR and the lab (with regards >to how expeditiously the Bx are fixed/delivered). > In our case we did the same two things but we really eliminated the >problem when we eliminated xylene in our tissue processing and changed to >mineral oil. > If you want I can send you that protocol. > Regards > Ren? J. > >Patricia Karlisch wrote: > All, >We are having occassional problems with small colon, stomach and >antrum specimens that are 'drying' out, at least this is what we think. >The specimens show no nuclear detail and the H&E looks smudgy. Other >specimens stained with the same slides look great. We process our small >specimens on a short 5 hour processing schedule which seemed to have >improved some of our 85% of our specimens. Has anyone else had this >problem? We think that the specimen may be sitting in the OR before >they get placed in formalin. Is there any way to fix this when it >happens? Any suggestions would be helpful. Pat Karlisch > >Pat Karlisch >Supervisor, Histology, Pathology and Laboratory Medicine >Penn State Milton S. Hershey Medical Center >Mail Code H179 >Hershey, PA 17033 >Phone (717) 531-6072 >Fax: (717) 531- 7741 >email: pkarlisch@psu.edu > >*****E-Mail Confidentiality Notice***** >This message (including any attachments) contains information intended >for a specific individual(s) and purpose that may be privileged, >confidential or otherwise protected from disclosure pursuant to >applicable law. Any inappropriate use, distribution or copying of the >message is strictly prohibited and may subject you to criminal or civil >penalty. If you have received this transmission in error, please reply >to the sender indicating this error and delete the transmission from >your system immediately. > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > >--------------------------------- >Stay in the know. Pulse on the new Yahoo.com. Check it out. >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Back to school shopping is as easy as 1-2-3 http://shopping.sympatico.msn.ca/content/shp/?ctId=493,ptnrid=176,ptnrdata=081803 --------------------------------- Talk is cheap. Use Yahoo! Messenger to make PC-to-Phone calls. Great rates starting at 1¢/min. From bayoubelle311 <@t> gmail.com Wed Aug 30 10:38:06 2006 From: bayoubelle311 <@t> gmail.com (Missy) Date: Wed Aug 30 10:38:12 2006 Subject: [Histonet] reticulin stain confusion Message-ID: <398f02c20608300838o44940078m623bbf5e7cf7491e@mail.gmail.com> Hi all, I am slightly confused with a stain I am anticipating on using. It was suggested that I use Gomori's silver stain to visualize and quantify reticulin deposits in bone marrow. I have done a few searches and am confused as to wether Gomori silver is a stain or a method. If it is a method, has anyone here used it and does it work well? Missy From hakim <@t> bgu.ac.il Wed Aug 30 10:40:27 2006 From: hakim <@t> bgu.ac.il (Vicki Hakim) Date: Wed Aug 30 10:40:56 2006 Subject: [Histonet] Small endoscopy specimens In-Reply-To: References: <20060829165312.83183.qmail@web61211.mail.yahoo.com> Message-ID: hi i would like to also join my request for that protocol we are currently trying to deal with that kind of a problem and this is one of the few things that i haven't tried thanks vicki ----- Original Message ----- From: sheila adey Date: Wednesday, August 30, 2006 17:33 Subject: Re: [Histonet] Small endoscopy specimens To: rjbuesa@yahoo.com, pkarlisch@psu.edu, histonet@lists.utsouthwestern.edu > Hi, > Do you use mineral oil on your processor for large specimens to? > I would > like to see your protocol aswell. We have on occasion had this > same problem. > Thanks > Sheila HT, MLT > Port Huron Hospital > > > > >From: Rene J Buesa > >To: Patricia Karlisch , > >histonet@lists.utsouthwestern.edu > >Subject: Re: [Histonet] Small endoscopy specimens > >Date: Tue, 29 Aug 2006 09:53:12 -0700 (PDT) > > > >Patricia: > > You yourself have pointed out to the 2 main > sources of the problem: > > 1- a longer than needed processing schedule is > one. Reducing it, > >specially the dehydrating stations, can help, and > > 2- doing a better coordination between the OR and > the lab (with regards > >to how expeditiously the Bx are fixed/delivered). > > In our case we did the same two things but we > really eliminated the > >problem when we eliminated xylene in our tissue processing and > changed to > >mineral oil. > > If you want I can send you that protocol. > > Regards > > Ren? J. > > > >Patricia Karlisch wrote: > > All, > >We are having occassional problems with small colon, stomach and > >antrum specimens that are 'drying' out, at least this is what > we think. > >The specimens show no nuclear detail and the H&E looks smudgy. Other > >specimens stained with the same slides look great. We process > our small > >specimens on a short 5 hour processing schedule which seemed to have > >improved some of our 85% of our specimens. Has anyone else had this > >problem? We think that the specimen may be sitting in the OR before > >they get placed in formalin. Is there any way to fix this when it > >happens? Any suggestions would be helpful. Pat Karlisch > > > >Pat Karlisch > >Supervisor, Histology, Pathology and Laboratory Medicine > >Penn State Milton S. Hershey Medical Center > >Mail Code H179 > >Hershey, PA 17033 > >Phone (717) 531-6072 > >Fax: (717) 531- 7741 > >email: pkarlisch@psu.edu > > > >*****E-Mail Confidentiality Notice***** > >This message (including any attachments) contains information > intended>for a specific individual(s) and purpose that may be > privileged,>confidential or otherwise protected from disclosure > pursuant to > >applicable law. Any inappropriate use, distribution or copying > of the > >message is strictly prohibited and may subject you to criminal > or civil > >penalty. If you have received this transmission in error, > please reply > >to the sender indicating this error and delete the transmission from > >your system immediately. > > > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > > > > >--------------------------------- > >Stay in the know. Pulse on the new Yahoo.com. Check it out. > >_______________________________________________ > >Histonet mailing list > >Histonet@lists.utsouthwestern.edu > >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _________________________________________________________________ > Back to school shopping is as easy as 1-2-3 > http://shopping.sympatico.msn.ca/content/shp/?ctId=493,ptnrid=176,ptnrdata=081803 > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ? From PMonfils <@t> Lifespan.org Wed Aug 30 10:56:57 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Aug 30 10:57:07 2006 Subject: [Histonet] reticulin stain confusion Message-ID: <09C945920A6B654199F7A58A1D7D1FDE01717793@lsexch.lsmaster.lifespan.org> Gomori's reticulin stain is a method that employs an ammoniacal silver nitrate solution, not unlike several other reticulin methods. Paul M. From PMonfils <@t> Lifespan.org Wed Aug 30 10:58:14 2006 From: PMonfils <@t> Lifespan.org (Monfils, Paul) Date: Wed Aug 30 10:58:18 2006 Subject: [Histonet] reticulin stain confusion - P.S. Message-ID: <09C945920A6B654199F7A58A1D7D1FDE01717794@lsexch.lsmaster.lifespan.org> P.S. - I can send you the protocol if you need it. Paul M. > ---------- > From: histonet-bounces@lists.utsouthwestern.edu on behalf of Missy > Sent: Wednesday, August 30, 2006 8:38 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] reticulin stain confusion > > Hi all, > > I am slightly confused with a stain I am anticipating on using. It was > suggested that I use Gomori's silver stain to visualize and quantify > reticulin deposits in bone marrow. I have done a few searches and am > confused as to wether Gomori silver is a stain or a method. If it is a > method, has anyone here used it and does it work well? > > Missy > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > From rjbuesa <@t> yahoo.com Wed Aug 30 11:24:27 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Aug 30 11:24:30 2006 Subject: [Histonet] reticulin stain confusion In-Reply-To: <398f02c20608300838o44940078m623bbf5e7cf7491e@mail.gmail.com> Message-ID: <20060830162427.22864.qmail@web61215.mail.yahoo.com> Missy: Gomori is one of many ammoniacal silver methods. The one you are referring to was developed by Gomori in 1937 and it has been my preferred method for reticulin for many years. You also mentione that you want to visualize and QUANTIFY reticulin deposits in BM. For starters you will have to use at least 7?m thick sections to get an idea of the reticulin mesh interelations, otherwise you will only see fragments without interrelations. This is with regards to visualizing another thing is quantifiying. How do you intend to do that? Quantification is extremely difficult for a reticulin mesh. The only thing I could do is to give an approximate evaluation of number of trabecules/objective field and even this is really quite problematic. You could try to measure thickness of the trabecules or the distance between them. I am really at a loss as to how you could quantify a reticulin mesh. Recently there have attempts of quatififying glycogen contents and it has been used the same type of Olympus photometer used for photomicrography to get an idea of optical density, and even this approach has been hampered by inconsistencies in section thickness and quality of the PAS reagent and staining protocol. I have a paper on "Quantifying Quality" that I could send you if you want me to. Hope this will help you Ren? J. Missy wrote: Hi all, I am slightly confused with a stain I am anticipating on using. It was suggested that I use Gomori's silver stain to visualize and quantify reticulin deposits in bone marrow. I have done a few searches and am confused as to wether Gomori silver is a stain or a method. If it is a method, has anyone here used it and does it work well? Missy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- How low will we go? Check out Yahoo! Messenger?s low PC-to-Phone call rates. From nancy.troiano <@t> yale.edu Wed Aug 30 12:42:21 2006 From: nancy.troiano <@t> yale.edu (Nancy W. Troiano) Date: Wed Aug 30 12:42:24 2006 Subject: [Histonet] GMA technovit 7100 Message-ID: <5.2.1.1.2.20060830133705.00c329d8@email.med.yale.edu> Deanna - one of the first things that you can check is your benzoyl peroxide - is it fresh? Have you completely dissolved the benzoyl peroxide before adding the hardener II? In the humid summer months GMA will tend to absorb water and the blocks can be soft - I usually cover the embedding molds (we embed in Peel-A- Way molds) tightly with foil during polymerization and polymerize in a closed container in the fridge in which I also put a cassette containing Dri Rite (or any other dessicant). If your blocks are soft you can also put them in a 37 deg C oven to harden. Also, I store my polymerized GMA blocks in a closed container with dessicant. Hope this helps! From laurie.colbert <@t> huntingtonhospital.com Wed Aug 30 13:37:10 2006 From: laurie.colbert <@t> huntingtonhospital.com (Laurie Colbert) Date: Wed Aug 30 13:37:18 2006 Subject: [Histonet] VIP Problem Message-ID: <0BE6ADFAE4E7E04496BF21ABD346628006307D09@EXCHANGE1.huntingtonhospital.com> We had a problem with our VIP 5 the other night, and our service rep can't find anything wrong with it. There is no error code showing to indicate what might have happened. I'm wondering if anyone has had a similar problem. When the pathologist put the blocks on the processor on Sunday, she said the retort was very hot - hotter than usual. When our tech went in Monday morning, he said the formalin smell was overbearing when he opened the retort. The blocks had been in paraffin, but they looked dry and they were not processed well at all (we had to reprocess everything). We add eosin to the 95% alcohol so that the tissue will be colored, but the tissue was not colored at all on this day, so we're pretty sure they didn't go into the 95% alcohol - and probably not into any of the alcohols. We also discovered a little bit of formalin in the last paraffin. I've heard of condensation forming on the lid of the retort and dripping down into the solutions, and this may have happened, but something else also happened. Does anyone have any ideas?? Laurie Colbert Huntington Hospital From kimtournear <@t> yahoo.com Wed Aug 30 13:55:42 2006 From: kimtournear <@t> yahoo.com (Kim Tournear) Date: Wed Aug 30 13:55:46 2006 Subject: [Histonet] Theresa Schuldt....OKC...are you out there???? Message-ID: <20060830185542.98425.qmail@web37701.mail.mud.yahoo.com> Hey Theresa, Are you out there in Histo land.....please contact me at this address regarding the histo program in Tucson AZ.....or at work 520-382-3366..... Kim Tournear, HT (ASCP), QIHC (ASCP) Specialists in Dermatology Tucson, AZ --------------------------------- Get your own web address for just $1.99/1st yr. We'll help. Yahoo! Small Business. From int09018 <@t> alphahunt.com Wed Aug 30 14:08:26 2006 From: int09018 <@t> alphahunt.com (HCS) Date: Wed Aug 30 14:08:33 2006 Subject: [Histonet] re:solutions for CEM stain Message-ID: <000601c6cc67$ad1e4de0$6601a8c0@hp> Would anyone have the protocol for making up CEM stain solutions or perhaps a modified version of this stain that works well? The main solutions are Astra Blue and Vital New Red, as well as, the Modified Mayer's Hematoxylin used in this stain. I have found very few remarks about this stain posted on the histonet or by doing a web search. Thanks for your help. LeRoy Brown HT(ASCP) HTL HCS www.histocs.com From rjbuesa <@t> yahoo.com Wed Aug 30 14:27:25 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Wed Aug 30 14:27:31 2006 Subject: [Histonet] VIP Problem In-Reply-To: <0BE6ADFAE4E7E04496BF21ABD346628006307D09@EXCHANGE1.huntingtonhospital.com> Message-ID: <20060830192725.63676.qmail@web61222.mail.yahoo.com> Laurie: Never mind the "error code" and tell the Sakura representative to run a "mock run" (without times) to check the rotary valve. Maybe this is the problem and it is stuck in the "pre-paraffin" step (when the retort has to be a 63?C). Ren? J. Laurie Colbert wrote: We had a problem with our VIP 5 the other night, and our service rep can't find anything wrong with it. There is no error code showing to indicate what might have happened. I'm wondering if anyone has had a similar problem. When the pathologist put the blocks on the processor on Sunday, she said the retort was very hot - hotter than usual. When our tech went in Monday morning, he said the formalin smell was overbearing when he opened the retort. The blocks had been in paraffin, but they looked dry and they were not processed well at all (we had to reprocess everything). We add eosin to the 95% alcohol so that the tissue will be colored, but the tissue was not colored at all on this day, so we're pretty sure they didn't go into the 95% alcohol - and probably not into any of the alcohols. We also discovered a little bit of formalin in the last paraffin. I've heard of condensation forming on the lid of the retort and dripping down into the solutions, and this may have happened, but something else also happened. Does anyone have any ideas?? Laurie Colbert Huntington Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Get your own web address for just $1.99/1st yr. We'll help. Yahoo! Small Business. From pkarlisch <@t> psu.edu Wed Aug 30 18:01:59 2006 From: pkarlisch <@t> psu.edu (Patricia Karlisch) Date: Wed Aug 30 18:02:37 2006 Subject: [Histonet] VIP Problem In-Reply-To: <0BE6ADFAE4E7E04496BF21ABD346628006307D09@EXCHANGE1.huntingtonhospital.com> References: <0BE6ADFAE4E7E04496BF21ABD346628006307D09@EXCHANGE1.huntingtonhospital.com> Message-ID: <44F5E0A70200008C00020FA9@GWIA02.HERSHEYMED.NET> Laurie, Did you try to do a 1minute run through each reagent to see if the machine is pumping correctly? We have 3 VIP's and have not had this problem yet. Pat Karlisch Pat Karlisch Supervisor, Histology, Pathology and Laboratory Medicine Penn State Milton S. Hershey Medical Center Mail Code H179 Hershey, PA 17033 Phone (717) 531-6072 Fax: (717) 531- 7741 email: pkarlisch@psu.edu *****E-Mail Confidentiality Notice***** This message (including any attachments) contains information intended for a specific individual(s) and purpose that may be privileged, confidential or otherwise protected from disclosure pursuant to applicable law. Any inappropriate use, distribution or copying of the message is strictly prohibited and may subject you to criminal or civil penalty. If you have received this transmission in error, please reply to the sender indicating this error and delete the transmission from your system immediately. >>> "Laurie Colbert" 8/30/2006 2:37 PM >>> We had a problem with our VIP 5 the other night, and our service rep can't find anything wrong with it. There is no error code showing to indicate what might have happened. I'm wondering if anyone has had a similar problem. When the pathologist put the blocks on the processor on Sunday, she said the retort was very hot - hotter than usual. When our tech went in Monday morning, he said the formalin smell was overbearing when he opened the retort. The blocks had been in paraffin, but they looked dry and they were not processed well at all (we had to reprocess everything). We add eosin to the 95% alcohol so that the tissue will be colored, but the tissue was not colored at all on this day, so we're pretty sure they didn't go into the 95% alcohol - and probably not into any of the alcohols. We also discovered a little bit of formalin in the last paraffin. I've heard of condensation forming on the lid of the retort and dripping down into the solutions, and this may have happened, but something else also happened. Does anyone have any ideas?? Laurie Colbert Huntington Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From int09018 <@t> alphahunt.com Wed Aug 30 19:07:52 2006 From: int09018 <@t> alphahunt.com (HCS) Date: Wed Aug 30 19:08:01 2006 Subject: [Histonet] CEM stain Message-ID: <000a01c6cc91$809c77e0$6601a8c0@hp> The question was asked "what is CEM?" This is a "new"? stain, at least new to me that stains eosinophils and mast cells. CEM stands for Combined eosinophil mast cell stain. See questions below. LeRoy Brown Histology Consultation Services PO Box 770 207 N. Harkness St. Everson, WA 98247 www.histocs.com ----- Original Message ----- From: HCS To: Histonet Sent: Wednesday, August 30, 2006 12:08 PM Subject: re:solutions for CEM stain Would anyone have the protocol for making up CEM stain solutions or perhaps a modified version of this stain that works well? The main solutions are Astra Blue and Vital New Red, as well as, the Modified Mayer's Hematoxylin used in this stain. I have found very few remarks about this stain posted on the histonet or by doing a web search. Thanks for your help. LeRoy Brown HT(ASCP) HTL HCS www.histocs.com From jnocito <@t> satx.rr.com Wed Aug 30 21:18:16 2006 From: jnocito <@t> satx.rr.com (Joe Nocito) Date: Wed Aug 30 21:16:23 2006 Subject: [Histonet] VIP Problem References: <0BE6ADFAE4E7E04496BF21ABD346628006307D09@EXCHANGE1.huntingtonhospital.com> Message-ID: <003901c6cca3$bb868a50$63614542@yourxhtr8hvc4p> I bet it's the rotary valve. For the longest time we were getting formalin in our xylenes. Turned out to be the rotary valve. Good luck. Joe Nocito BS, HT(ASCP)QIHC Histology Manager Pathology Reference Lab San Antonio, TX ----- Original Message ----- From: "Laurie Colbert" To: Sent: Wednesday, August 30, 2006 1:37 PM Subject: [Histonet] VIP Problem We had a problem with our VIP 5 the other night, and our service rep can't find anything wrong with it. There is no error code showing to indicate what might have happened. I'm wondering if anyone has had a similar problem. When the pathologist put the blocks on the processor on Sunday, she said the retort was very hot - hotter than usual. When our tech went in Monday morning, he said the formalin smell was overbearing when he opened the retort. The blocks had been in paraffin, but they looked dry and they were not processed well at all (we had to reprocess everything). We add eosin to the 95% alcohol so that the tissue will be colored, but the tissue was not colored at all on this day, so we're pretty sure they didn't go into the 95% alcohol - and probably not into any of the alcohols. We also discovered a little bit of formalin in the last paraffin. I've heard of condensation forming on the lid of the retort and dripping down into the solutions, and this may have happened, but something else also happened. Does anyone have any ideas?? Laurie Colbert Huntington Hospital _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- No virus found in this incoming message. Checked by AVG Free Edition. Version: 7.1.405 / Virus Database: 268.11.7/433 - Release Date: 8/30/2006 From drmoses111 <@t> comcast.net Thu Aug 31 07:25:31 2006 From: drmoses111 <@t> comcast.net (drmoses111@comcast.net) Date: Thu Aug 31 07:25:35 2006 Subject: [Histonet] Histology Position in South Jersey Message-ID: <083120061225.28431.44F6D53B0005E7BB00006F0F2200760180CECECE9C0A9C01039D0B@comcast.net> Our lab has an opening for a registered histology technician. We are a large cummunity hospital system, with excelent salary and benefits. Please e-mail me if interested at : drmoses111@comcast.net Renee Boston-Moses, HT QIHC From dfvilleg <@t> mtu.edu Thu Aug 31 09:01:36 2006 From: dfvilleg <@t> mtu.edu (Diego Villegas) Date: Thu Aug 31 09:01:45 2006 Subject: [Histonet] Collagen fiber in ligaments Message-ID: <44F6EBC0.4050904@mtu.edu> I am working with cow knee ligaments. Basically, I am trying to look the collagen fiber structure and quantify the GAGs. Someone has a protocol for its histology?. The area of the samples is about 15 mm x 20 mm. Diego Villegas MTU-Biomechanics Lab. From sheila_adey <@t> hotmail.com Thu Aug 31 09:08:57 2006 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Thu Aug 31 09:09:06 2006 Subject: [Histonet] Lab Vysion staining system Message-ID: Could anyone offer pros or cons on this IHC staining system? Thanks _________________________________________________________________ Back to school shopping is as easy as 1-2-3 http://shopping.sympatico.msn.ca/content/shp/?ctId=493,ptnrid=176,ptnrdata=081803 From m.doube <@t> qmul.ac.uk Thu Aug 31 09:19:46 2006 From: m.doube <@t> qmul.ac.uk (Michael Doube) Date: Thu Aug 31 09:19:54 2006 Subject: [Histonet] Parts for Laborlux 12 Message-ID: <44F6F002.2020003@qmul.ac.uk> Hi All I acquired a Leitz-Wetzlar Laborlux 12 microscope with fluorescence attachments during a recent pathology lab clear-out. It's a bit old but in good condition, so I am renovating it. Does anyone have a trinocular head, with C mount or SLR camera (esp. Canon) that would be suitable for this unit, that they are willing to part with or swap for the binocular head? Know anyone who might? Thanks, Mike -- Michael Doube BPhil BVSc MRCVS PhD Student Dental Institute Queen Mary, University of London New Rd London E1 1BB United Kingdom Phone +44 (0)20 7377 7000 ext 2681 From graham.brown <@t> ttuhsc.edu Thu Aug 31 09:38:02 2006 From: graham.brown <@t> ttuhsc.edu (Brown, Graham W) Date: Thu Aug 31 09:36:41 2006 Subject: [Histonet] Immunohistochem help! Message-ID: <7F7DE44DEE269C4CB34BB8EADE0525EC61E440@BOWIE.ttuhsc.edu> Dear All- I'm a student with a little bit of experience in immunohistochemistry. I am familiar with using paraffin sections and fresh frozen sections, however i've never stained any tissue that was fixed prior to freezing. I was wondering if anyone has a general protocol for staining fixed (with formalin) frozen tissue. Is it better to use fresh tissue for frozens rather than using fixed frozen tissues? Thanks in advance! Graham Brown Texas Tech University Health Sciences Center From sheila_adey <@t> hotmail.com Thu Aug 31 09:39:25 2006 From: sheila_adey <@t> hotmail.com (sheila adey) Date: Thu Aug 31 09:39:35 2006 Subject: [Histonet] Deparaffinization question Message-ID: Hello Histonetters. I am curious about how long other labs are spending in Xylene when bringing tissue to water? I started here 6 years ago and never questioned the rehydration times but I think we may not be spending enough time in Xylene. Our times are 1 min in each of 2 xylenes. This week we increased it to 5 minutes in each and I think our IHCs are staining better? Any input? Thanks Sheila Adey HT, MLT Port Huron Michigan _________________________________________________________________ Back to school shopping is as easy as 1-2-3 http://shopping.sympatico.msn.ca/content/shp/?ctId=493,ptnrid=176,ptnrdata=081803 From msviapiano <@t> yahoo.com Thu Aug 31 09:59:44 2006 From: msviapiano <@t> yahoo.com (Mariano S. Viapiano) Date: Thu Aug 31 09:59:55 2006 Subject: [Histonet] Microscopes. Message-ID: <20060831145944.2458.qmail@web54511.mail.yahoo.com> Hello everybody, This is my first message to the forum; I am a complete newbie, not only in the forum but in the field of histopathology as well (I come from a background in molecular biology). I intend to buy a couple of very simple microscopes for routine examination of cells and specimens. Those microscopes will be used in addition to major imaging equipment (Zeiss / Nikon), so they don?t need to belong to major brands or be the top of the line. I've found a number of brands in catalogs, such as VanGuard, Jenco or Acculab, for which I have no previous experience. Moreover, they all seem to come from the same producer and have only different brand labels. Does anyone have experience with microscopes from those brands, and would care to comment? Thank you very much, Mariano. Mariano S. Viapiano, PhD Center for Molecular Neurobiology Ohio State University 226B Rightmire Hall 1060 Carmack Rd., Columbus OH 43210 Tel (614) 292-4362 Fax (614) 292-5379 __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com From Janet.Bonner <@t> FLHOSP.ORG Thu Aug 31 10:08:42 2006 From: Janet.Bonner <@t> FLHOSP.ORG (Bonner, Janet) Date: Thu Aug 31 10:09:57 2006 Subject: [Histonet] Deparaffinization question References: Message-ID: Yes, five minutes in each xylene is our protocol and we use 3 xylenes. Janet ________________________________ From: histonet-bounces@lists.utsouthwestern.edu on behalf of sheila adey Sent: Thu 8/31/2006 10:39 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Deparaffinization question Hello Histonetters. I am curious about how long other labs are spending in Xylene when bringing tissue to water? I started here 6 years ago and never questioned the rehydration times but I think we may not be spending enough time in Xylene. Our times are 1 min in each of 2 xylenes. This week we increased it to 5 minutes in each and I think our IHCs are staining better? Any input? Thanks Sheila Adey HT, MLT Port Huron Michigan _________________________________________________________________ Back to school shopping is as easy as 1-2-3 http://shopping.sympatico.msn.ca/content/shp/?ctId=493,ptnrid=176,ptnrdata=081803 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From Terry.Marshall <@t> rothgen.nhs.uk Thu Aug 31 10:25:26 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Thu Aug 31 10:29:53 2006 Subject: [Histonet] Lyme disease agent staining Message-ID: I have a case which may be Lyme disease, manifesting as erythema chronicum migrans, the first skin manifestation. Has anyone had any experience? If so, what silver stain did you use to demonstrate the organism? Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk From mcauliff <@t> umdnj.edu Thu Aug 31 10:37:39 2006 From: mcauliff <@t> umdnj.edu (Geoff McAuliffe) Date: Thu Aug 31 10:37:07 2006 Subject: [Histonet] Microscopes. In-Reply-To: <20060831145944.2458.qmail@web54511.mail.yahoo.com> References: <20060831145944.2458.qmail@web54511.mail.yahoo.com> Message-ID: <44F70243.8000508@umdnj.edu> Dear Mariano: I suggest you check with the Anatomy/Neurobiology/Cell Biology dept. at the medical school and see if they have a surplus microscope they will lend or sell to you. Geoff Mariano S. Viapiano wrote: >Hello everybody, >This is my first message to the forum; I am a complete >newbie, not only in the forum but in the field of >histopathology as well (I come from a background in >molecular biology). >I intend to buy a couple of very simple microscopes >for routine examination of cells and specimens. Those >microscopes will be used in addition to major imaging >equipment (Zeiss / Nikon), so they don?t need to >belong to major brands or be the top of the line. I've >found a number of brands in catalogs, such as >VanGuard, Jenco or Acculab, for which I have no >previous experience. Moreover, they all seem to come >from the same producer and have only different brand >labels. Does anyone have experience with microscopes >from those brands, and would care to comment? >Thank you very much, >Mariano. > > > >Mariano S. Viapiano, PhD >Center for Molecular Neurobiology >Ohio State University >226B Rightmire Hall >1060 Carmack Rd., Columbus OH 43210 >Tel (614) 292-4362 >Fax (614) 292-5379 > >__________________________________________________ >Do You Yahoo!? >Tired of spam? Yahoo! Mail has the best spam protection around >http://mail.yahoo.com > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583; fax: -4029 mcauliff@umdnj.edu ********************************************** From Terry.Marshall <@t> rothgen.nhs.uk Thu Aug 31 11:15:30 2006 From: Terry.Marshall <@t> rothgen.nhs.uk (Marshall Terry Dr, Consultant Histopathologist) Date: Thu Aug 31 11:20:49 2006 Subject: [Histonet] bowel biopsies (or whatever it was) Message-ID: My previous post contained a URL which may not have been allowed in all e-mail clients. Second attempt:-) Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path Consultant Pathologist Rotherham General Hospital South Yorkshire England terry.marshall@rothgen.nhs.uk http://makeashorterlink.com/?G1E132BAD From rjbuesa <@t> yahoo.com Thu Aug 31 11:23:38 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Aug 31 11:23:42 2006 Subject: [Histonet] Deparaffinization question In-Reply-To: Message-ID: <20060831162338.52212.qmail@web61218.mail.yahoo.com> Sheila: Deparafinization is a fundamental step for IHC. If paraffin is not completely removed it will compromise IHC reactions. In a recent study on deparafinizing agents it was found that octane was the best but is not practical because of health hazards and costs. Short of that, xylene is more than adequate but you have to make sure it completely works. I used to have 3 xylene stations and the slides stayed 5 minutes in each. Xylenes in the last 2 stations were moved forward after 20 slides were treated and fresh xylene was added to the last station (much like moving reagents in a tissue processor). Hope this will help you. Ren? J. sheila adey wrote: Hello Histonetters. I am curious about how long other labs are spending in Xylene when bringing tissue to water? I started here 6 years ago and never questioned the rehydration times but I think we may not be spending enough time in Xylene. Our times are 1 min in each of 2 xylenes. This week we increased it to 5 minutes in each and I think our IHCs are staining better? Any input? Thanks Sheila Adey HT, MLT Port Huron Michigan _________________________________________________________________ Back to school shopping is as easy as 1-2-3 http://shopping.sympatico.msn.ca/content/shp/?ctId=493,ptnrid=176,ptnrdata=081803 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Why keep checking for Mail? The all-new Yahoo! Mail shows you when there are new messages. From m-degutes <@t> northwestern.edu Thu Aug 31 11:29:34 2006 From: m-degutes <@t> northwestern.edu (Mathew DeGutes) Date: Thu Aug 31 11:29:38 2006 Subject: [Histonet] OCT embedded Tissue Storage Message-ID: <20060831162934.4F30C35D3A@casbah.it.northwestern.edu> I have a couple questions. In the lab I work in we have started to accumulate a number of experiments worth of tissue blocks that we would like to keep for reference at later dates. Generally tissue survives better while still embedded in a block than when in slide form, so I was curious as to what sorts of methods others here employ to keep order and good preservation of blocks in -80s? Up until now we have been just keeping individual sets of blocks in Ziplock bags, but it has gotten to be unwieldy. I am looking for any suggestions of a good way to keep the blocks well preserved and easily recovered in -80s. From rjbuesa <@t> yahoo.com Thu Aug 31 11:29:47 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Aug 31 11:29:51 2006 Subject: [Histonet] Microscopes. In-Reply-To: <20060831145944.2458.qmail@web54511.mail.yahoo.com> Message-ID: <20060831162947.28650.qmail@web61224.mail.yahoo.com> Mariano: If you go to www.sciplus.com you will find fantastic new microscopes at very competitive prices. They are manufactured in Russia by LOMO which is a reputable optical equipment factory in Leningrad. You will be buying a Carl Zeiss Jena microscope, since this factory was established with the machinery the Soviet Red Army confiscated from the Jena factory as war "compensation" in 1945. Check them out, I am sure you will like them. Hope this will help you. Ren? J. Hello everybody, This is my first message to the forum; I am a complete newbie, not only in the forum but in the field of histopathology as well (I come from a background in molecular biology). I intend to buy a couple of very simple microscopes for routine examination of cells and specimens. Those microscopes will be used in addition to major imaging equipment (Zeiss / Nikon), so they don?t need to belong to major brands or be the top of the line. I've found a number of brands in catalogs, such as VanGuard, Jenco or Acculab, for which I have no previous experience. Moreover, they all seem to come from the same producer and have only different brand labels. Does anyone have experience with microscopes from those brands, and would care to comment? Thank you very much, Mariano. Mariano S. Viapiano, PhD Center for Molecular Neurobiology Ohio State University 226B Rightmire Hall 1060 Carmack Rd., Columbus OH 43210 Tel (614) 292-4362 Fax (614) 292-5379 __________________________________________________ Do You Yahoo!? Tired of spam? Yahoo! Mail has the best spam protection around http://mail.yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Want to be your own boss? Learn how on Yahoo! Small Business. From AGrobe2555 <@t> aol.com Thu Aug 31 11:36:07 2006 From: AGrobe2555 <@t> aol.com (AGrobe2555@aol.com) Date: Thu Aug 31 11:36:15 2006 Subject: [Histonet] Deparaffinization Times Message-ID: <42c.5ceb4ca.322869f7@aol.com> You could also check after immersion in the first alcohol. The tissues that are clear of paraffin become more opaque while you will see spots/patches that look different if all the wax isn't out. This can be another indicator of sufficient time in Xylene. When I worked with paraffin sections years ago, we would do at least 3-5 minutes in 3 separate xylene baths. 1 minute in 2 xylenes sounds a bit short....but may ultimately depend on the type and thickness of tissue. Albert C. Grobe, PhD International Heart Institute, Tissue Engineering Lab Saint Patrick Hospital From rjbuesa <@t> yahoo.com Thu Aug 31 11:50:28 2006 From: rjbuesa <@t> yahoo.com (Rene J Buesa) Date: Thu Aug 31 11:50:32 2006 Subject: [Histonet] OCT embedded Tissue Storage In-Reply-To: <20060831162934.4F30C35D3A@casbah.it.northwestern.edu> Message-ID: <20060831165028.65482.qmail@web61214.mail.yahoo.com> Mathew: In my lab we used to have a "tissue back" for teaching/reference purposes. All tissues were kep in OCT at -80?C in a freezer, and we used to have tissues more than 15 years old. When needed, they were FS without any problem. Ren? J. Mathew DeGutes wrote: I have a couple questions. In the lab I work in we have started to accumulate a number of experiments worth of tissue blocks that we would like to keep for reference at later dates. Generally tissue survives better while still embedded in a block than when in slide form, so I was curious as to what sorts of methods others here employ to keep order and good preservation of blocks in -80s? Up until now we have been just keeping individual sets of blocks in Ziplock bags, but it has gotten to be unwieldy. I am looking for any suggestions of a good way to keep the blocks well preserved and easily recovered in -80s. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. From TJJ <@t> Stowers-Institute.org Thu Aug 31 12:57:52 2006 From: TJJ <@t> Stowers-Institute.org (Johnson, Teri) Date: Thu Aug 31 12:58:08 2006 Subject: [Histonet] Re: Immunohistochem help! Message-ID: Graham, the short answer is: it depends (on whether the antigen you're trying to demonstrate is recognized by the antibody after formalin fixation). For most of our IHC in frozen section, we use formalin fixed and sucrose cryoprotected frozen sections. We do use antigen retrieval for some of the antibodies, notably BrdU and GFP, but at 60 degrees C for 10 minutes. Some epitopes (proteins) become more stable with fixation prior to immunostaining, while others undergo enough conformational changes that the antibody does not recognize it. In many cases polyclonal antibodies might be more "forgiving" and therefore easier to detect the protein regardless of the pre-IHC processing. The general protocol we use is basically the same as with paraffin section with three notable differences: 1 - no deparaffinization step 2 - Antigen retrieval is done at a lower temp (always test with and without - sometimes you will destroy the signal using AR) 3 - H2O2 quenching is done using 0.3% hydrogen peroxide in buffer or water for 30 minutes @ RT. All other procedural steps are the same. Hope this helps! Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 Dear All- I'm a student with a little bit of experience in immunohistochemistry. I am familiar with using paraffin sections and fresh frozen sections, however i've never stained any tissue that was fixed prior to freezing. I was wondering if anyone has a general protocol for staining fixed (with formalin) frozen tissue. Is it better to use fresh tissue for frozens rather than using fixed frozen tissues? Thanks in advance! Graham Brown Texas Tech University Health Sciences Center Teri Johnson, HT(ASCP)QIHC Managing Director Histology Facility Stowers Institute for Medical Research 1000 E. 50th St. Kansas City, MO 64110 From jessgrocki <@t> yahoo.com Thu Aug 31 13:02:42 2006 From: jessgrocki <@t> yahoo.com (Jessica Piche) Date: Thu Aug 31 13:02:46 2006 Subject: [Histonet] Job Message-ID: <20060831180242.90191.qmail@web82001.mail.mud.yahoo.com> Hi Everyone, Just wanted to let you know that there is a job in Histology posted at www.waterburyhospital.org . Thanks, Jessica Piche-Grocki, HT(ASCP) From SHARON.OSBORN <@t> SPCORP.COM Thu Aug 31 13:08:13 2006 From: SHARON.OSBORN <@t> SPCORP.COM (Osborn, Sharon) Date: Thu Aug 31 13:08:20 2006 Subject: [Histonet] Frozen tissue storage Message-ID: <9A919A5D70313A4D9C56A025710874080C739D@kenmsg40.us.schp.com> Mathew, There are systems you can purchase to store tissues or OCT blocks in an orderly fashion in -80 freezers. We place individual blocks in Fisher Twirl 'Em sterile sampling bags 3x5 inches, label and place in a correctly sized cardboard box that then fits into stainless shelves. These shelves hold 12 boxes each stacked in 4 rows of 3 boxes each. Four shelves will fit onto one shelf of a -80. The Freezer is numbered, the boxes are numbered and the racks are numbered ie.. 5/10/48 would interpret as freezer #5, rack # 10 and Box #48. This is an example of what you could set up for storing your tissues. As Rene mentioned,tissues will keep as long as they are properly preserved, sealed and the -80 freezers do not go down; or when the freezer goes down, someone rescues its contents BEFORE everything melts--those emergency contacts again! I also have cut frozen sections from tissues that have been frozen for years. I have also cut paraffin sections from blocks over 20 years old that cut well when properly stored. I have also had some that the bugs had dinner from so there was no tssue left! Okay, Joe, your turn! :-) Sharon Osborn DNAX, SPBioPharma Palo Alto, CA Mathew DeGutes wrote: I have a couple questions. In the lab I work in we have started to accumulate a number of experiments worth of tissue blocks that we would like to keep for reference at later dates. Generally tissue survives better while still embedded in a block than when in slide form, so I was curious as to what sorts of methods others here employ to keep order and good preservation of blocks in -80s? Up until now we have been just keeping individual sets of blocks in Ziplock bags, but it has gotten to be unwieldy. I am looking for any suggestions of a good way to keep the blocks well preserved and easily recovered in -80s. ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. From jennifer.l.hofecker <@t> Vanderbilt.Edu Thu Aug 31 13:49:49 2006 From: jennifer.l.hofecker <@t> Vanderbilt.Edu (Hofecker, Jennifer L) Date: Thu Aug 31 13:49:54 2006 Subject: [Histonet] Frozen tissue storage References: <9A919A5D70313A4D9C56A025710874080C739D@kenmsg40.us.schp.com> Message-ID: <898D946569A27444B65667A49C074052852EC9@mailbe06.mc.vanderbilt.edu> Hi Matthew, I am an alumni of the ziplock bags full of "goodies" in the -80 freezer, too! We implemented a new system last year. We got 30 ml specimen cups from Surgipath (may get from another vendor too). They are actually the same vials that come prefilled or prelabeled. We were able to get them with no labels and no formalin. They come in a box of 50 that is the exact same size as the depth of our (upright) freezer shelf. We wrap the OCT in foil and then label one of the specimen cups with the number ( on the lid too) Then we label the outside of the box. We can open the door, grab a specimen and be out of the freezer before you know it. If you have a surgipath rep, they can probably get you a sample box (that's how we fell in love...) BEFORE THE FLAMING: I know there are oodles of vendors of specimen containers. This box size is perfect and there is a cardboard insert inside that makes it super easy to take one out and put it back in the same spot. Good Luck and Have a great Rest of the week! Jennifer Jennifer Hofecker, HT (ASCP) Vanderbilt University Medical Center Division of Neuropathology (615) 343-0083 (615) 343-7089 fax Mathew DeGutes wrote: I have a couple questions. In the lab I work in we have started to accumulate a number of experiments worth of tissue blocks that we would like to keep for reference at later dates. Generally tissue survives better while still embedded in a block than when in slide form, so I was curious as to what sorts of methods others here employ to keep order and good preservation of blocks in -80s? Up until now we have been just keeping individual sets of blocks in Ziplock bags, but it has gotten to be unwieldy. I am looking for any suggestions of a good way to keep the blocks well preserved and easily recovered in -80s. ********************************************************************* This message and any attachments are solely for the intended recipient. If you are not the intended recipient, disclosure, copying, use or distribution of the information included in this message is prohibited -- Please immediately and permanently delete. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet From tkngflght <@t> yahoo.com Thu Aug 31 13:52:34 2006 From: tkngflght <@t> yahoo.com (Cheryl) Date: Thu Aug 31 13:52:39 2006 Subject: [Histonet] OCT embedded Tissue Storage In-Reply-To: <20060831165028.65482.qmail@web61214.mail.yahoo.com> Message-ID: <20060831185235.32327.qmail@web50914.mail.yahoo.com> Hi! I worked at a research lab that had limited space but horded tissue like gold (because it was like gold!!). We cut a square of foil (the precut pop-up kind from the cafeteria works fine) and another from parafilm. We stuck a paper label to the block with OCT, knocked the block off the chuck, sealed it in the parafilm (labeled in Sharpie) then wrapped it in the foil sealing the edges by rolling and pressing them and afixing a label freezer sticker (test your regular labels, they may work). It was awesome because the size of the tissue/OCT block when wrapped fit into the cardboard block file drawers with the outer boxes now in popular use. The outer boxes stayed in the freezer so we could access all blocks without digging through piles or ziplock bags full of stuff and they fit tight and stayed cold even when pulling them out to handle them. When sorting through boxes, we pulled them out onto our ice trays with foil on them to keep the box dry and allowing a longer open time. Like Rene mentions--sealing is the most important bit. And not loosing the ID. Hope this helps! Cheryl Rene J Buesa wrote: Mathew: In my lab we used to have a "tissue back" for teaching/reference purposes. All tissues were kep in OCT at -80?C in a freezer, and we used to have tissues more than 15 years old. When needed, they were FS without any problem. Ren? J. Mathew DeGutes wrote: I have a couple questions. In the lab I work in we have started to accumulate a number of experiments worth of tissue blocks that we would like to keep for reference at later dates. Generally tissue survives better while still embedded in a block than when in slide form, so I was curious as to what sorts of methods others here employ to keep order and good preservation of blocks in -80s? Up until now we have been just keeping individual sets of blocks in Ziplock bags, but it has gotten to be unwieldy. I am looking for any suggestions of a good way to keep the blocks well preserved and easily recovered in -80s. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet --------------------------------- Do you Yahoo!? Everyone is raving about the all-new Yahoo! Mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Cheryl Kerry, HT(ASCP) Full Staff Inc. Staffing the AP Lab by helping one Tech at a time. 281.883.7704 c 281.852.9457 o admin@fullstaff.org Sign up for the FREE monthly newsletter AP News--updates, tricks of the trade and current issues for Anatomic Pathology Clinical Labs. Send a 'subscribe' request to APNews@fullstaff.org. Please include your name and specialty in the body of the email.