[Histonet] Antigen retreival on mouse GFAP.

Geoff McAuliffe mcauliff <@t> umdnj.edu
Fri Sep 23 09:15:06 CDT 2005


Hi Susan:

    I don't know why you would need antigen retrieval for GFAP on mouse 
brain. Most people I know use DAKO's rabbit anti-cow antibody, catalogue 
# Z0334. It works very well on both fixed, frozen sections and on 
formalin-fixed wax embedded tissue. With a Vector ABC Elite kit my 
dilution for GFAP primary is 1:2000 or even more dilute, depending on 
how I intensify the DAB. I also get great results with fluorescence. 
Perhaps your trouble is somewere else?

Geoff

Susan.Ferrigon <@t> sanofi-aventis.com wrote:

>Hi
>
>Does anyone have any experience with using trypsin as the method of antigen
>retreival when staining GFAP on mouse brains???  Any advice on
>concentration, temperature and incubation time would be appreciated.
>
>Thanks
>
>Susan.
>
>----------------------------------------------------------
>Le présent message ainsi que ses éventuelles pièces jointes est
>exclusivement destiné au(x) destinataire(s), personnes physiques ou
>morales, qu'il désigne.
>Il constitue de ce fait une correspondance à caractère privé et peut
>contenir des informations confidentielles.
>Si ce message vous est parvenu par erreur, nous vous remercions d'en aviser
>immédiatement l'expéditeur par retour de courrier électronique puis de le
>détruire, ainsi que ses éventuelles pièces jointes, sans en conserver de
>copie.
>
>This message, including any attachment, is intended for the use of the
>individual or entity to which it is addressed.
>It is therefore to be considered as a private correspondence which may
>contain confidential information.
>If you are not the intended recipient, please advise the sender immediately
>by reply e.mail and delete this message and any attachment thereto without
>retaining a copy.
>----------------------------------------------------------
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>  
>


-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu
**********************************************





More information about the Histonet mailing list