[Histonet] bone fish samples embedded in Historesin Plus

[Gayle Callis]

It may be better to do the sectioning of these samples using a tungsten 
carbide tipped knife , d profile should work.  A regular steel c profile 
knife will not cut through undecalcified bones very well.  It take the 
extremely hard TC knife for this purpose.  Since Historesin Plus is GMA - 
you may want to enter into some discussion about this plastic and 
sectioning with "Patsy Ruegg" <pruegg <@t> ihctech.net> as she has experience 
using this kind of knife with undecalcified bone embedded in GMA.


  At 10:28 AM 11/9/2005, you wrote:


>We are using samples without decalcification because we are studing the bone
>fish development.
>
>Historesin is glycol metacrylate based (manufactured by Leica).
>

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)