[Histonet] Mounting Gelatine Embedded Cryostat CNS Sections

[germckeon <@t> excite.com]

Hello,

I am currently working on spinal cord and am having trouble with sections floating off. I have embedded with gelatine, am performing cryostat sectioning and will be doing some of the more structural stains eg Kultschitsky's stain for myelin. I have been using Superfrost slides.

Can anyone advise me regarding choices of slides, coatings, methodologies, etc which would maximise adherence?



In gratitude for any advice received,

Gerald McKeon







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