[Histonet] decalcification

[Matt Prideaux]

We decalcify our mouse bones in EDTA, usually for about 4 weeks before
processing and embedding. It's a fairly slow process but it seems to preserve
the enzymes and antigens in the tissue far better than any of the acid
decalcifying methods.

If you're going to be doing a lot of bone histology you might want to check out
the 'Handbook of Histology Methods for Bone and Cartilage' by Y.H. An and K.L.
Martin. We've found it invaluable so far.

Matt

-- 
Matt Prideaux
Academic Unit of Bone Biology
Division of Clinical Sciences (South)
D Floor (DU20) Medical School
Henry Wellcome Laboratories for Medical Research
University of Sheffield
Beech Hill Road
Sheffield 
S10 2RX





Quoting pex <pex0220 <@t> yahoo.com.cn>:

> Dear all,
>  
> I have a question about decalcification. I have got some bone tissues 
> (such as femur) from newborn pups, I would like to know if they need to 
> be decalcified before paraffin embedding.
>  
> Thanks a lot.
>  
> Guofeng
> 
> 
> 		
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