[Histonet] CD44 v4 and v7

[Mcleod]

Dear Histonetters

A colleague is having trouble with these 2 antibodies.  She has tried
hier at ph 6
and 8, enzyme ar and no ar without any success.  Her problem is specific
stainig
amidst lots of non-specific staining and when the dilution is increased
the desired signal is lost and she is left with all of the nss.
If anybody has successfully worked with these antibodies; any advice /
help would
be appreciated.
She is using Novocastra CD44 v4 and 
Serotec CD44 v7 (MCA1731)

Many many thanks.

Heather