[Histonet] cutting angle

Traczyk7 <@t> aol.com Traczyk7 <@t> aol.com
Wed May 4 16:07:26 CDT 2005


Hi Alan,
Help me out here, are you saying that the knife gauge angle and  clearance 
angle are the same?  If not, then does it really matter  that the gauge markings 
are accurate?   Setting a 1  to 3 degree clearance angle is still a problem.  
Can't very well stick  my head in the cryostat chamber to view the 
block/knife interface from the  end.  And of course there is then the problem of the 
bevel angle of the  blade varying between manufacturers.  Also, you mentioned 
"flat" specimen  face but wouldn't having the block parallel to the knife edge be 
important  also?
I have successfully set up many cryostats, in this life and in my past  lives 
as both a rep and a tech.  I have never been able to determine a  universal 
method for adjusting knife angle but welcome any advice that will  make me look 
brilliant to my customers.
Thanks,
Dorothy
 



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