[Histonet] F4/80 on human tumour xenografts in mouse

[Corazon D. Bucana]

We published a paper in 1992 entitled:  Different patterns of macrophage 
infiltration into allogeneic-murine and xenogeneic-human neoplasms growing 
in nude mice.  Bucana et al. Am J Pathol 1992 Nov : 141(5):1225-36.

At 04:22 PM 3/11/2005 +0000, you wrote:
>Hello,
>
>Has anyone tried murine F4/80 IHC staining on human tumour xenografts grown
>in nude mice to look for infiltrating macrophages?  We are using the Caltag
>rat monoclonal, BM8, although we've got it working nicely on normal mouse
>spleen, the staining pattern is unusual in the tumour xenografts, staining
>is seen around the human tumour cells (almost like tumour matrix) rather
>than infiltrating mouse cells.  Has anyone seen this pattern before or can
>suggest what is happening here?
>
>Thank you in advance,
>
>Nicola
>
>Nicola Cragg BSc
>Epistem Ltd
>Manchester, UK
>
>
>
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